Yeast-like ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES isolated from exuded tree sap.
A colorless, flammable liquid used in the manufacture of FORMALDEHYDE and ACETIC ACID, in chemical synthesis, antifreeze, and as a solvent. Ingestion of methanol is toxic and may cause blindness.
Proteins prepared by recombinant DNA technology.
Protein factors released from one species of YEAST that are selectively toxic to another species of yeast.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
An order of fungi in the phylum Ascomycota that multiply by budding. They include the telomorphic ascomycetous yeasts which are found in a very wide range of habitats.

Kodamaea nitidulidarum, Candida restingae and Kodamaea anthophila, three new related yeast species from ephemeral flowers. (1/1453)

Three new yeast species were discovered during studies of yeasts associated with ephemeral flowers in Brazil, Australia and Hawaii. Their physiological and morphological similarity to Kodamaea (Pichia) ohmeri suggested a possible relationship to that species, which was confirmed by rDNA sequencing. Kodamaea nitidulidarum and Candida restingae were found in cactus flowers and associated nitidulid beetles in sand dune ecosystems (restinga) of South-eastern Brazil. Over 350 strains of Kodamaea anthophila were isolated from Hibiscus and morning glory flowers (Ipomoea spp.) in Australia, and from associated nitidulid beetles and Drosophila hibisci. A single isolate came from a beach morning glory in Hawaii. Expansion of the genus Kodamaea to three species modified the existing definition of the genus only slightly. The type and isotype strains are as follows: K. nitidulidarum strains UFMG96-272T (h+; CBS 8491T) and UFMG96-394I (h-; CBS 8492I); Candida restingae UFMG96-276T (CBS 8493T); K. anthophila strains UWO(PS)95-602.1T (h+; CBS 8494T), UWO(PS)91-893.2I (h-; CBS 8495I) and UWO(PS)95-725.1I (h-; CBS 8496I).  (+info)

Glycosylation of asparagine-28 of recombinant staphylokinase with high-mannose-type oligosaccharides results in a protein with highly attenuated plasminogen activator activity. (2/1453)

The properties of recombinant staphylokinase (SakSTAR) expressed in Pichia pastoris cells have been determined. The single consensus N-linked oligosaccharide linkage site in SakSTAR (at Asn28 of the mature protein) was occupied in approximately 50% of the expressed protein with high-mannose-type oligosaccharides. The majority of these glycans ranged in polymerization state from Man8GlcNAc2 to Man14GlcNAc2, with the predominant species being Man10GlcNAc2 and Man11GlcNAc2. Glycosylated SakSTAR (SakSTARg) did not differ from its aglycosyl form in its aggregation state in solution, its thermal denaturation properties, its ability to form a complex with human plasmin (hPm), the amidolytic properties of the respective SakSTAR-hPm complexes, or its ability to liberate the amino-terminal decapeptide required for formation of a functional SakSTAR-hPm plasminogen activator complex. However, this latter complex with SakSTARg showed a greatly reduced ability to activate human plasminogen (hPg) as compared with the same complex with the aglycosyl form of SakSTAR. We conclude that glycosylation at Asn28 does not affect the structural properties of SakSTAR or its ability to participate in the formation of an active enzymatic complex with hPm, but it is detrimental to the ability of the SakSTAR-hPm complex to serve as a hPg activator. This is likely due to restricted access of hPg to the active site of the SakSTARg-hPm complex.  (+info)

Protection of mice against a lethal influenza virus challenge after immunization with yeast-derived secreted influenza virus hemagglutinin. (3/1453)

The A/Victoria/3/75 (H3N2-subtype) hemagglutinin (HA) gene was engineered for expression in Pichia pastoris as a soluble secreted molecule. The HA cDNA lacking the C-terminal transmembrane anchor-coding sequence was fused to the Saccharomyces cerevisiae alpha-mating factor secretion signal and placed under control of the methanol-inducible P. pastoris alcohol oxidase 1 (AOX1) promoter. Growth of transformants on methanol-containing medium resulted in the secretion of recombinant non-cleaved soluble hemagglutinin (HA0s). Remarkably, the pH of the induction medium had an important effect on the expression level, the highest level being obtained at pH 8.0. The gel filtration profile and the reactivity against a panel of different HA-conformation specific monoclonal antibodies indicated that HA0s was monomeric. Analysis of the N-linked glycans revealed a typical P. pastoris type of glycosylation, consisting of glycans with 10-12 glycosyl residues. Mice immunized with purified soluble hemagglutinin (HA0s) showed complete protection against a challenge with 10 LD50 of mouse-adapted homologous virus (X47), whereas all control mice succumbed. Heterologous challenge with X31 virus [A/Aichi/2/68 (H3N2-subtype)], resulted in significantly higher survival rates in the immunized group compared with the control group. These results, together with the safety, reliability and economic potential of P. pastoris, as well as the flexibility and fast adaptation of the expression system may allow development of an effective recombinant influenza vaccine.  (+info)

Cloning, mutagenesis, and structural analysis of human pancreatic alpha-amylase expressed in Pichia pastoris. (4/1453)

Human pancreatic alpha-amylase (HPA) was expressed in the methylotrophic yeast Pichia pastoris and two mutants (D197A and D197N) of a completely conserved active site carboxylic acid were generated. All recombinant proteins were shown by electrospray ionization mass spectrometry (ESI-MS) to be glycosylated and the site of attachment was shown to be Asn461 by peptide mapping in conjunction with ESI-MS. Treatment of these proteins with endoglycosidase F demonstrated that they contained a single N-linked oligosaccharide and yielded a protein product with a single N-acetyl glucosamine (GlcNAc), which could be crystallized. Solution of the crystal structure to a resolution of 2.0 A confirmed the location of the glycosyl group as Asn461 and showed that the recombinant protein had essentially the same conformation as the native enzyme. The kinetic parameters of the glycosylated and deglycosylated wild-type proteins were the same while the k(cat)/Km values for D197A and D197N were 10(6)-10(7) times lower than the wild-type enzyme. The decreased k(cat)/Km values for the mutants confirm that D197 plays a crucial role in the hydrolytic activity of HPA, presumably as the catalytic nucleophile.  (+info)

The N-terminal CUB-epidermal growth factor module pair of human complement protease C1r binds Ca2+ with high affinity and mediates Ca2+-dependent interaction with C1s. (5/1453)

The Ca2+-dependent interaction between complement serine proteases C1r and C1s is mediated by their alpha regions, encompassing the major part of their N-terminal CUB-EGF-CUB (where EGF is epidermal growth factor) module array. In order to define the boundaries of the C1r domain(s) responsible for Ca2+ binding and Ca2+-dependent interaction with C1s and to assess the contribution of individual modules to these functions, the CUB, EGF, and CUB-EGF fragments were expressed in eucaryotic systems or synthesized chemically. Gel filtration studies, as well as measurements of intrinsic Tyr fluorescence, provided evidence that the CUB-EGF pair adopts a more compact conformation in the presence of Ca2+. Ca2+-dependent interaction of intact C1r with C1s was studied using surface plasmon resonance spectroscopy, yielding KD values of 10.9-29.7 nM. The C1r CUB-EGF pair bound immobilized C1s with a higher KD (1.5-1.8 microM), which decreased to 31.4 nM when CUB-EGF was used as the immobilized ligand and C1s was free. Half-maximal binding was obtained at comparable Ca2+ concentrations ranging from 5 microM with intact C1r to 10-16 microM for C1ralpha and CUB-EGF. The isolated CUB and EGF fragments or a CUB + EGF mixture did not bind C1s. These data demonstrate that the C1r CUB-EGF module pair (residues 1-175) is the minimal segment required for high affinity Ca2+ binding and Ca2+-dependent interaction with C1s and indicate that Ca2+ binding induces a more compact folding of the CUB-EGF pair.  (+info)

Cloning, expression, and properties of a nonneuronal secreted acetylcholinesterase from the parasitic nematode Nippostrongylus brasiliensis. (6/1453)

We have isolated a full-length cDNA encoding an acetylcholinesterase secreted by the nematode parasite Nippostrongylus brasiliensis. The predicted protein is truncated in comparison with acetylcholinesterases from other organisms such that the carboxyl terminus aligns closely to the end of the catalytic domain of the vertebrate enzymes. The residues in the catalytic triad are conserved, as are the six cysteines which form the three intramolecular disulfide bonds. Three of the fourteen aromatic residues which line the active site gorge in the Torpedo enzyme are substituted by nonaromatic residues, corresponding to Tyr-70 (Thr), Trp-279 (Asn), and Phe-288 (Met). High level expression was obtained via secretion from Pichia pastoris. The purified enzyme behaved as a monomeric hydrophilic species. Although of invertebrate origin and possessing the above substitutions in the active site gorge residues, the enzyme efficiently hydrolyzed acetylthiocholine and showed minimal activity against butyrylthiocholine. It displayed excess substrate inhibition with acetylthiocholine at concentrations over 2. 5 mM and was highly sensitive to both active site and "peripheral" site inhibitors. Northern blot analysis indicated a progressive increase in mRNA for AChE B in parasites isolated from 6 days postinfection.  (+info)

Positive selection of novel peroxisome biogenesis-defective mutants of the yeast Pichia pastoris. (7/1453)

We have developed two novel schemes for the direct selection of peroxisome-biogenesis-defective (pex) mutants of the methylotrophic yeast Pichia pastoris. Both schemes take advantage of our observation that methanol-induced pex mutants contain little or no alcohol oxidase (AOX) activity. AOX is a peroxisomal matrix enzyme that catalyzes the first step in the methanol-utilization pathway. One scheme utilizes allyl alcohol, a compound that is not toxic to cells but is oxidized by AOX to acrolein, a compound that is toxic. Exposure of mutagenized populations of AOX-induced cells to allyl alcohol selectively kills AOX-containing cells. However, pex mutants without AOX are able to grow. The second scheme utilizes a P. pastoris strain that is defective in formaldehyde dehydrogenase (FLD), a methanol pathway enzyme required to metabolize formaldehyde, the product of AOX. AOX-induced cells of fld1 strains are sensitive to methanol because of the accumulation of formaldehyde. However, fld1 pex mutants, with little active AOX, do not efficiently oxidize methanol to formaldehyde and therefore are not sensitive to methanol. Using these selections, new pex mutant alleles in previously identified PEX genes have been isolated along with mutants in three previously unidentified PEX groups.  (+info)

Physiological effects and adjuvanticity of recombinant brushtail possum TNF-alpha. (8/1453)

The present paper describes the physiological properties of recombinant possum TNF-alpha and an adjuvant effect on antibody responses to the model protein antigen, keyhole limpet haemocyanin (KLH). For these studies recombinant possum TNF-alpha was produced in the yeast Pichia pastoris. The recombinant cytokine was secreted into the culture medium and purified by gel filtration. Possum TNF-alpha produced in this expression system was N-glycosylated and bioactive in two different assays. In a murine fibroblast L929 cytotoxicity assay, the possum TNF-alpha had lower specific activity compared to human TNF-alpha, while in a possum-specific assay, possum TNF-alpha enhanced the proliferation of PHA-stimulated possum thymocytes and was more active than human TNF-alpha. The physiological effect of the recombinant possum TNF-alpha was investigated in groups of possums administered doses of 6, 30 or 150 micrograms of cytokine. For each dose, TNF-alpha caused profound effects on the numbers of circulating leucocytes characterized by a three-to-four-fold increase in neutrophil numbers at 6-24 h after injection and an initial sharp decrease in lymphocyte numbers. The efficacy of TNF-alpha as an immunological adjuvant was determined in possums administered KLH (125 micrograms) in an aqueous or Al(OH)3-based formulation with or without added recombinant TNF-alpha (150 micrograms). Serum antibody responses to KLH were monitored by ELISA. The TNF-alpha stimulated two-fold and four-fold increases in antibody levels in aqueous and Al(OH)3-based vaccine formulations, respectively. The strongest antibody responses were observed in the group of possums that received KLH formulated in Al(OH)3 with addition of TNF-alpha.  (+info)

"Pichia" is a genus of single-celled yeast organisms that are commonly found in various environments, including on plant and animal surfaces, in soil, and in food. Some species of Pichia are capable of causing human infection, particularly in individuals with weakened immune systems. These infections can include fungemia (bloodstream infections), pneumonia, and urinary tract infections.

Pichia species are important in a variety of industrial processes, including the production of alcoholic beverages, biofuels, and enzymes. They are also used as model organisms for research in genetics and cell biology.

It's worth noting that Pichia was previously classified under the genus "Candida," but it has since been reclassified due to genetic differences between the two groups.

Methanol, also known as methyl alcohol or wood alcohol, is a volatile, colorless, flammable liquid with a distinctive odor similar to that of ethanol (drinking alcohol). It is used in various industrial applications such as the production of formaldehyde, acetic acid, and other chemicals. In the medical field, methanol is considered a toxic alcohol that can cause severe intoxication and metabolic disturbances when ingested or improperly consumed. Methanol poisoning can lead to neurological symptoms, blindness, and even death if not treated promptly and effectively.

Recombinant proteins are artificially created proteins produced through the use of recombinant DNA technology. This process involves combining DNA molecules from different sources to create a new set of genes that encode for a specific protein. The resulting recombinant protein can then be expressed, purified, and used for various applications in research, medicine, and industry.

Recombinant proteins are widely used in biomedical research to study protein function, structure, and interactions. They are also used in the development of diagnostic tests, vaccines, and therapeutic drugs. For example, recombinant insulin is a common treatment for diabetes, while recombinant human growth hormone is used to treat growth disorders.

The production of recombinant proteins typically involves the use of host cells, such as bacteria, yeast, or mammalian cells, which are engineered to express the desired protein. The host cells are transformed with a plasmid vector containing the gene of interest, along with regulatory elements that control its expression. Once the host cells are cultured and the protein is expressed, it can be purified using various chromatography techniques.

Overall, recombinant proteins have revolutionized many areas of biology and medicine, enabling researchers to study and manipulate proteins in ways that were previously impossible.

I cannot find a specific medical term called "killer factors" in the context of yeast. However, there is a phenomenon known as "killer yeasts," where certain strains of yeast produce proteins that can kill other strains of yeast. These proteins are referred to as "killer toxins."

Killer yeasts have been found in various species, including Saccharomyces cerevisiae and Saccharomyces bayanus. The killer toxin produced by these yeasts targets specific receptors on the cell membrane of sensitive yeast cells, leading to ion imbalance, disruption of cellular processes, and eventually cell death.

Therefore, "killer factors" in the context of yeast may refer to the genetic elements or proteins that enable certain strains of yeast to produce killer toxins and kill other sensitive yeast cells.

Molecular sequence data refers to the specific arrangement of molecules, most commonly nucleotides in DNA or RNA, or amino acids in proteins, that make up a biological macromolecule. This data is generated through laboratory techniques such as sequencing, and provides information about the exact order of the constituent molecules. This data is crucial in various fields of biology, including genetics, evolution, and molecular biology, allowing for comparisons between different organisms, identification of genetic variations, and studies of gene function and regulation.

Molecular cloning is a laboratory technique used to create multiple copies of a specific DNA sequence. This process involves several steps:

1. Isolation: The first step in molecular cloning is to isolate the DNA sequence of interest from the rest of the genomic DNA. This can be done using various methods such as PCR (polymerase chain reaction), restriction enzymes, or hybridization.
2. Vector construction: Once the DNA sequence of interest has been isolated, it must be inserted into a vector, which is a small circular DNA molecule that can replicate independently in a host cell. Common vectors used in molecular cloning include plasmids and phages.
3. Transformation: The constructed vector is then introduced into a host cell, usually a bacterial or yeast cell, through a process called transformation. This can be done using various methods such as electroporation or chemical transformation.
4. Selection: After transformation, the host cells are grown in selective media that allow only those cells containing the vector to grow. This ensures that the DNA sequence of interest has been successfully cloned into the vector.
5. Amplification: Once the host cells have been selected, they can be grown in large quantities to amplify the number of copies of the cloned DNA sequence.

Molecular cloning is a powerful tool in molecular biology and has numerous applications, including the production of recombinant proteins, gene therapy, functional analysis of genes, and genetic engineering.

Saccharomycetales is an order of fungi that are commonly known as "true yeasts." They are characterized by their single-celled growth and ability to reproduce through budding or fission. These organisms are widely distributed in nature and can be found in a variety of environments, including soil, water, and on the surfaces of plants and animals.

Many species of Saccharomycetales are used in industrial processes, such as the production of bread, beer, and wine. They are also used in biotechnology to produce various enzymes, vaccines, and other products. Some species of Saccharomycetales can cause diseases in humans and animals, particularly in individuals with weakened immune systems. These infections, known as candidiasis or thrush, can affect various parts of the body, including the skin, mouth, and genital area.

T. Seki 2005 Pichia nakasei J.A. Barnett, R.W. Payne & Yarrow 1983 Pichia nanzhaoensis F.L. Hui & K.F. Liu 2018 Pichia ... Pichia Species Archived 2014-07-02 at the Wayback Machine, Doctor Fungus, url accessed 2014-02-27 Pichia at Dr Fungus Pichia in ... M. Miranda 1976 Pichia sporocuriosa G. Péter, Tornai-Leh., Dlauchy & Vitányi 2000 Pichia terricola Van der Walt 1957 Pichia ... 2018 Pichia heedii Phaff, Starmer, M. Miranda & M.W. Mill. 1978 Pichia kluyveri Bedford ex Kudryavtsev 1960 Pichia kudriavzevii ...
... is a species of yeast. Lee MD, Creagh JW, Fredericks LR, Crabtree AM, Patel JS, Rowley PA (March 2022 ... "The Characterization of a Novel Virus Discovered in the Yeast Pichia membranifaciens". Viruses. 14 (3): 594. doi:10.3390/ ...
... is a species of yeast in the family Saccharomycetaceae. Described in 1978, it was found growing on a dead senita ... Pichia heedii in MycoBank. v t e (Articles with short description, Short description matches Wikidata, Articles with 'species' ... Phaff HJ, Starmer WT, Miranda M, Miller MW (1978). "Pichia heedii, a new species of yeast indigenous to necrotic cacti in the ...
... is a budding yeast (a species of fungus) involved in chocolate production. Candida krusei is an emerging ... Candida krusei is the anamorph name; the teleomorph name for the same organism is Pichia kudriavzevii. The International ... have proposed revising the standard name to Pichia kudriavzevii in 2021. Candida krusei can be successfully treated with ...
Higgins, D. R., & Cregg, J. M. (1998). "Introduction to Pichia pastoris". Pichia Protocols. Methods in Molecular Biology. Vol. ... It was found in the 1960s as Pichia pastoris, with its feature of using methanol as a source of carbon and energy. In 1995, P. ... 2000). "Modeling Pichia pastoris growth on methanol and optimizing the production of a recombinant protein, the heavy‐chain ... Cregg JM, Tolstorukov I, Kusari A, Sunga J, Madden K, Chappell T (2009). "Chapter 13 Expression in the Yeast Pichia pastoris". ...
"Pichia anomala , Viticulture & Enology". wineserver.ucdavis.edu. Retrieved 2016-04-16. "Oxygen- and Glucose-Dependent ... Madrigal, T.; Maicas, S.; Mateo Tolosa, J. J. (2013-03-01). "Glucose and Ethanol Tolerant Enzymes Produced by Pichia ( ... Distinguished from some other species of Pichia by high osmotolerance, P. anomala ferments sucrose, and assimilates raffinose. ... Regulation of Central Carbon Metabolism in Pichia anomala". aem.asm.org. Retrieved 2016-04-16. ...
Su, Lin-Hui; Ou, Jonathan T.; Leu, Hsieh-Shong; Chiang, Ping-Cherng; Chiu, Yueh-Pi; Chia, Ju-Hsin; Kuo, An-Jing; Chiu, Cheng- ...
Some specialized yeasts, e.g., Candida tropicale, Pichia sp., Rhodotorula sp., can use alkanes as a source of carbon or energy ...
A yeast commonly used for protein production is Pichia pastoris. Examples of yeast expression vector in Pichia are the pPIC ... "Pichia pastoris Expression System" (PDF). Invitrogen. "K. lactis Protein Expression Kit" (PDF). New England BioLabs Inc. Fields ... Cregg JM, Cereghino JL, Shi J, Higgins DR (2000). "Recombinant protein expression in Pichia pastoris". Molecular Biotechnology ...
Expression systems using either S. cerevisiae or Pichia pastoris allow stable and lasting production of proteins that are ... Cregg JM, Cereghino JL, Shi J, Higgins DR (September 2000). "Recombinant protein expression in Pichia pastoris". Molecular ...
Menotti Del Pichia Juliana Carneiro da Cunha...foreign dancer (allusion to Isadora Duncan) Dina Sfat...Branca Clara (allusion ...
In a study from Cordeiro et al (2022) they expressed recombinant Ts8 (rTs8) in Pichia pastoris yeast to be able to evaluate the ... This resulted in growth inhibition of Pichia pastoris. The native form of the toxin had a larger inhibitory effect than the ...
In the yeast species Pichia pastoris, lysyl oxidase constitutes a homodimeric structure. Each monomer consists of an active ... "The crystal structure of Pichia pastoris lysyl oxidase". Biochemistry. 42 (51): 15148-57. doi:10.1021/bi035338v. PMID 14690425 ...
He leads a team of scientists engaged in the studies of Pichia pastoris, a methylotrophic yeast species, with regard to its ... "Transcriptional interference in the methylotrophic yeast, Pichia pastoris". IISER, Thiruvananthapuram. 2012. Archived from the ... Pichia pastoris at Indian Institute of Science Education and Research, Thiruvananthapuram in October 2012. He was the co- ... and pyruvate carboxylase-deficient conditions in Pichia pastoris". Microbiology. 157 (12): 3361-3369. doi:10.1099/mic.0.053488- ...
nov., a new sibling species of Komagataella (Pichia) pastoris". Antonie van Leeuwenhoek. 111 (7): 1197-1207. doi:10.1007/s10482 ...
The protein is produced by the yeast Pichia pastoris; the process is similar to that of existing Hepatitis B vaccines. In April ...
Yeasts belonging to the genera Candida, Pichia, and Wickerhamomyces have been found in Aedes and Anopheles genera of mosquitoes ... 2012 Some genera have been re-assigned from Pichia family; such as W. canadensis, W. ciferri, W. lynferdii, W. salvicola and W ...
"GRAS Notification for Soybean Leghemoglobin Protein Derived from Pichia pastoris" (PDF). Food and Drug Administration. Archived ...
Murasugi A, Kido I, Kumai H, Asami Y (2003). "Efficient production of recombinant human pleio-trophin in yeast, Pichia pastoris ... Pichia pastoris". Protein Expr. Purif. 27 (2): 244-52. doi:10.1016/S1046-5928(02)00587-9. PMID 12597883. ...
Murasugi A, Kido I, Kumai H, Asami Y (2003). "Efficient production of recombinant human pleio-trophin in yeast, Pichia pastoris ...
Pichia pastoris does have stacked Golgi, while Saccharomyces cerevisiae does not. In plants, the individual stacks of the Golgi ...
Murphy KP, Gagne P, Pazmany C, Moody MD (March 1998). "Expression of human interleukin-17 in Pichia pastoris: purification and ...
"Tomographic Evidence for Continuous Turnover of Golgi Cisternae in Pichia pastoris". Molecular Biology of the Cell. 14 (6): ... "Tomographic evidence for continuous turnover of Golgi cisternae in Pichia pastoris". Molecular Biology of the Cell. 14 (6): ...
Murasugi A, Asami Y, Mera-Kikuchi Y (2001). "Production of recombinant human bile-salt-stimulated lipase in Pichia pastoris". ...
Feng Y, Cui LB, Liu CX, Ma QJ (2001). "[Inhibition effect in vitro of purified endostatin expressed in Pichia pastoris]". Sheng ...
Medium priority Scedosporium spp., Lomentospora prolificans, Coccidioides spp., Pichia kudriavzeveii (Candida krusei), ...
Pichia cactophila (1978) from decaying cacti and Drosophila flies feeding on them. Pichia heedii (1978) from the soft rot of ... Pichia opuntiae (1979) from the cladodes of Opuntia inermis in Australia and from the decaying parts of Cereoid cacti in North ... Starmer, W. T.; Phaff, H. J.; Miranda, M.; Miller, M. W. (1978). "Pichia cactophila, a New Species of Yeast Found in Decaying ... Phaff, H. J.; Starmer, W. T.; Miranda, M.; Miller, M. W. (1978). "Pichia heedii, a New Species of Yeast Indigenous to Necrotic ...
2002). "Functional expression of human liver cytosolic beta-glucosidase in Pichia pastoris. Insights into its role in the ...
... (formerly Pichia stipitis) is a species of yeast, belonging to the "CUG Clade" of ascomycetous yeasts ... March 2007). "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis". Nature ...
... synthetase from Pichia ciferrii". Bioprocess Biosyst Eng. 35 (1-2): 173-81. doi:10.1007/s00449-011-0640-x. PMID 21989639. S2CID ...
T. Seki 2005 Pichia nakasei J.A. Barnett, R.W. Payne & Yarrow 1983 Pichia nanzhaoensis F.L. Hui & K.F. Liu 2018 Pichia ... Pichia Species Archived 2014-07-02 at the Wayback Machine, Doctor Fungus, url accessed 2014-02-27 Pichia at Dr Fungus Pichia in ... M. Miranda 1976 Pichia sporocuriosa G. Péter, Tornai-Leh., Dlauchy & Vitányi 2000 Pichia terricola Van der Walt 1957 Pichia ... 2018 Pichia heedii Phaff, Starmer, M. Miranda & M.W. Mill. 1978 Pichia kluyveri Bedford ex Kudryavtsev 1960 Pichia kudriavzevii ...
Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies. Article DOI: 10.3791/58589-v • 05:30 ... Methanol Independent Expression Pichia Pastoris De-repression Technologies Recombinant Proteins Shake Flask Cultivation Up ... Journal / Bioengineering / Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies… ... The main advantage of this technique is that high level methanol free protein expression can be achieved using Pichia pastoris ...
Pichia membranifaciens Details. Pichia membranifaciens A licence fee may be applied to products purchased for commercial use. ... Pichia membranifaciens. Taxon Synonyms. Pichia membranifaciens Equivalent Strain Designations. NCTC 819 Depositor. A. Harden, ...
Chung, I.S.; Chun, J.K. 1994: Ethanol fermentation of Nafion-catalyzed hydrolysates of rice straw by Pichia stipitis CBS 5776 ... Nigam, J.N. 2001: Ethanol production from wheat straw hemicellulose hydrolysate by Pichia stipitis Journal of Biotechnology 87( ... Chen, S.-H.; Hwang, D.-R.; Chen, G.-H.; Hsu, N.-S.; Wu, Y.-T.; Li, T.-L.; Wong, C.-H. 2012: Engineering transaldolase in Pichia ... Awafo, V.; Chahal, D.; Simpson, B. 1998: Optimization of ethanol production by Saccharomyces cerevisiae (ATCC 60868) and Pichia ...
nbsp; Key words: Phytate, phytase, Pichia kudriavzevii TY13, yeast extract, wheat flour, cassava flour, sorghum flour, bread ... nbsp; Key words: Phytate, phytase, Pichia kudriavzevii TY13, yeast extract, wheat flour, cassava flour, sorghum flour, bread ... improve the phytate degradation in composite wheat-cassava-whole sorghum flour bread by adding a phytase releasing yeast Pichia ... improve the phytate degradation in composite wheat-cassava-whole sorghum flour bread by adding a phytase releasing yeast Pichia ...
The Top 5 Things to Know for Successful Pichia Fermentation. Several key factors are essential for successful fermentation of ... pichia pastoris. Here comes a brief and simple overview on what is needed for process optimization in terms of the capability ...
The present invention also provides Pichia strains, especially Pichia pastoris KCTC 0500BP, that are resistant to lactoferrin ... The present invention also provides Pichia strains, especially Pichia pastoris KCTC 0500BP, that are resistant to lactoferrin ... Justia Patents Recombinant Dna Technique Included In Method Of Making A Protein Or PolypeptideUS Patent for Pichia pastoris ... transforming a Pichia pastoris KCTC 0500BP cell with said vector, culturing the transformed cell, and recovering the ...
Transformation of pPICZaA-E2 to Pichia pastoris X-33 and Mut+ phenotype analysis F. Shabihah; F. Shabihah ... F. Shabihah, S. Pambudi, F. A. Sitepu, C. Ikhsan, B. Yohan, R. Lestari; Transformation of pPICZaA-E2 to Pichia pastoris X-33 ... Heterologous expression of Trichoderma reesei exoglucanase (Cel6A) in Pichia pastoris under the control of GAP promoter AIP ... Optimization of methanol induction for expression of synthetic gene Thermomyces lanuginosus lipase in Pichia pastoris AIP ...
6MWS32. P. pastoris. Pichia Pastoris. By: OfficinaeBio. Last edit: 9 months, 2 weeks ago. ...
Pscheidt, B., Schroer, K., Luef, K. P., Hartner, F. S., & Glieder, A. (2008). Engineered Pichia pastoris as platform for whole ... Engineered Pichia pastoris as platform for whole cell biotransformations. Beate Pscheidt, Kirsten Schroer, Klaus Peter Luef, ... Engineered Pichia pastoris as platform for whole cell biotransformations. Poster session presented at Advanced Course Applied ... Engineered Pichia pastoris as platform for whole cell biotransformations. / Pscheidt, Beate; Schroer, Kirsten; Luef, Klaus ...
Uzgoj kvasca Pichia stipitis na enzimskom hidrolizatu predobrađenog drveta bukve. Naslov (engleski). Cultivation of yeast ... "Uzgoj kvasca Pichia stipitis na enzimskom hidrolizatu predobrađenog drveta bukve." Diplomski rad, Sveučilište u Zagrebu, ... "Uzgoj kvasca Pichia stipitis na enzimskom hidrolizatu predobrađenog drveta bukve." Diplomski rad, Sveučilište u Zagrebu, ... Beštak, L. (2021). Uzgoj kvasca Pichia stipitis na enzimskom hidrolizatu predobrađenog drveta bukve (Diplomski rad). Zagreb: ...
One of which is the growing interest in Pichia pastoris for protein expression. Interestingly, you can find Pichia in raw milk ... Here is a guide for you to understand what Pichia pastoris is and all the science behind it:. What is Pichia pastoris?. Pichia ... Can Pichia pastoris be a model organism?. The Pichia pastoris is a good model organism. Like any other yeast, it has a short ... Why Pichia pastoris is important?. The Pichia pastoris is essential for biotech applications and biological research. The ...
Organizmem, który może już niedługo zacząć chronić nas przed ekspozycją na toksyny, jest przedstawiciel drożdży, Pichia anomala ...
Pichia pastoris just in time alternative respiration. Alexander Kern, Franz Stefan Hartner, Maria Elisabeth Freigaßner, M. ... Pichia pastoris just in time alternative respiration. / Kern, Alexander; Hartner, Franz Stefan; Freigaßner, Maria Elisabeth et ... title = "Pichia pastoris just in time alternative respiration",. author = "Alexander Kern and Hartner, {Franz Stefan} and ... Pichia pastoris just in time alternative respiration. In: Microbiology. 2007 ; No. 153(4). pp. 1250-1260. ...
Expression and characterization of Trichoderma reesei endoglucanase II in Pichia pastoris under the regulation of the GAP ... Expression of an endo-β-1,4-glucanase gene from orpinomyces PC-2 in Pichia pastoris. Int J Mol Sci. 12(5):3366-3380. doi: ... Pichia pastoris expression system : a potential candidate to ex- press protein in industrial and biopharmaceutical do- mains. ... In this study, the recombinant EGII protein was expressed in Pichia pastoris and it has a molecular mass of approximately 52 ...
Pichia pastoris GS115;Pichia pastoris X-33;Human chorionic gonadotropin;Human follicle stimulating hormone;Glycosylation; ... The methylotrophic yeast Pichia pastoris is widely used for the production of recombinant glycoproteins. With the aim to ... High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains-selection of strain GS115, and ... High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains-selection of strain GS115, and ...
Second generation Pichia pastoris strain and bioprocess designs Yeast was the first microorganism used by mankind for ... In the last decade, Pichia pastoris has become the leading eukaryotic host ... ...
... català español English ... Monitorització dels paràmetres respiratoris de cultius en bioreactors del llevat Pichia pastoris. ...
Check out videos that might be relevant for performing Protein isolation Yeast - Scheffersomyces (Pichia) stipitis using Y-PER ... Pichia) stipitis using Y-PER™ Yeast Protein Extraction Reagent from Thermo Fisher Scientific. ...
An efficient screen for peroxisome-deficient mutants of Pichia pastoris. / Liu, H.; Tan, X.; Veenhuis, M. et al. In: Journal of ... An efficient screen for peroxisome-deficient mutants of Pichia pastoris. H. Liu, X. Tan, M. Veenhuis, D. McCollum, J. M. Cregg ... An efficient screen for peroxisome-deficient mutants of Pichia pastoris. In: Journal of Bacteriology. 1992 ; Vol. 174, No. 15. ... Liu, H, Tan, X, Veenhuis, M, McCollum, D & Cregg, JM 1992, An efficient screen for peroxisome-deficient mutants of Pichia ...
Pichia kudriavzevii. Candida krusei. Pichia caribbica/Meyerozyma caribbica. Candida fermentati. Kodamaea ohmeri. Candida ...
In contrast, Pichia pastoris (formally reclassified as Komagataella phaffii, but simply called Pichia in this review) has ... Pichia. Yarrowia. Ustilago. CHO. t-Glc. 4.4 ± 0.4. 5.5 ± 0.3. 12.9 ± 2.7. 11.5 ± 0.2. 1.6 ± 0.2. 42.2 ± 2.5. 8.0 ± 1.0. 6.6 ± ... For instance, Pichia X-33 shows changes in its native cell wall polymers when grown in the rich YPD medium (Table 2) compared ... Doubling times (20 min to 24 h) were estimated based on work by Pichia [30], Yarrowia [31], Ustilago [32], Pseudomonas [33], ...
Pichia Pastoris (Type D - Recombinant) for molecular biology & PCR, 30U/mg. $74.66. - $5,505.98. ... Pichia Pastoris (Type D - Recombinant) for molecular biology & PCR, 30U/mg $74.66 - $5,505.98 ... Pichia Pastoris (Type D - Recombinant) for molecular biology & PCR, 30U/mg quantity. ...
The yeast Pichia pastoris expression system was investigated for the production of human cathepsin C (CatC) recombinant protein ... Dağlıoğlu, C. (2017). Cloning, expression, and activity analysis of human cathepsin C in the yeast pichia pastoris. Turkish ... Cloning, expression, and activity analysis of human cathepsin C in the yeast pichia pastoris. ...
Kulachkovsky A. R., Titorenko V. I., Sibirny A. A. Electron-Microscopic Examination of Pichia pinus Yeast Mutants with a Block ... Electron-Microscopic Examination of Pichia pinus Yeast Mutants with a Block of Different Stages of Ethanol Metabolism. ...
  • The methylotrophic yeast Pichia pastoris is widely used for the production of recombinant glycoproteins. (iisc.ac.in)
  • We describe a rapid and efficient screen for peroxisome-deficient (per) mutants in the yeast Pichia pastoris. (elsevierpure.com)
  • The yeast Pichia pastoris expression system was investigated for the production of human cathepsin C (CatC) recombinant protein. (iyte.edu.tr)
  • Over the last few decades the methylotrophic yeast Pichia pastoris has become a popular host for a wide range of products such as vaccines and therapeutic proteins. (biomedcentral.com)
  • In recent years the methylotrophic yeast Pichia pastoris has received wide acceptance for the production of biopharmaceuticals [ 3 ]. (biomedcentral.com)
  • World leaders in the field are betting on a new yield-enhancing expression technology using the yeast Pichia pastoris. (labiotech.eu)
  • The influence of aeration on ethanol production by Pichia stipitis was studied in wheat straw hydrolysates subjected to ozone pretreatment for the first time. (eurekamag.com)
  • Uzgoj kvasca Pichia stipitis na enzimskom hidrolizatu predobrađenog drveta bukve', Diplomski rad, Sveučilište u Zagrebu, Prehrambeno-biotehnološki fakultet, citirano: 26.09.2023. (unizg.hr)
  • Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Yeast - Scheffersomyces (Pichia) stipitis using Y-PER™ Yeast Protein Extraction Reagent from Thermo Fisher Scientific. (labettor.com)
  • Yeasts able to convert xylose into ethanol have been described like as Scheffersomyces (Pichia) stipitis, S. (Candida) shehatae and Pachysolen tannophilus . (scielo.br)
  • 7 7 Guo C, Jiang N. Physiological and enzymatic comparison between Pichia stipitis and recombinant Saccharomyces cerevisiae on xylose fermentation. (scielo.br)
  • Evaluation of Pichia stipitis cultivation strategies on rice straw hemicellulosic. (usp.br)
  • 1978 Pichia kluyveri Bedford ex Kudryavtsev 1960 Pichia kudriavzevii Biodin, Pignal & Besson 1965 Pichia megalospora Kuraishi 1958 Pichia membranifaciens E.C. Hansen 1904 Pichia methanothermo Minami & Yamamura 1978 Pichia myanmarensis Nagats. (wikipedia.org)
  • This study aimed to improve the phytate degradation in composite wheat-cassava-whole sorghum flour bread by adding a phytase releasing yeast Pichia kudriavzevii TY13 in baking, preincubated or not, with addition of yeast extract. (academicjournals.org)
  • The anamorphs of some Pichia species are Candida species. (wikipedia.org)
  • Included C. holmii , C. valida , Candida species, and Pichia species. (cdc.gov)
  • Zymosan, a cell wall preparation from Saccharomyces cerevisiae, is a potent stimulator of alveolar macrophages (AM). In the present study, preparations from the cell walls of Pichia fabianii, Candida sake, Trichosporon capitatum, Rhodotorula glutinis, and Cryptococcus laurentii were compared with zymosan and ss- 1-->3-glucan for their ability to stimulate AM and activate complement. (cdc.gov)
  • This method can help in the pre selection of chromosomes clones by Pichia pastoris strains expressing recombinant proteins under de-repressed conditions by a simple shake flask cultivations prior to up scaling. (jove.com)
  • The present invention also provides Pichia strains, especially Pichia pastoris KCTC 0500BP, that are resistant to lactoferrin polypeptides. (justia.com)
  • Powers 2008 Pichia fermentans Lodder (1932 Pichia galeiformis A. Endo & Goto 1987 Pichia garciniae Bhadra & Shivaji 2008 Pichia gijzeniarum M. Groenew. (wikipedia.org)
  • High level xylitol production by Pichia fermentans using non-detoxified xylose-rich sugarcane bagasse and olive pits hydrolysates. (bvsalud.org)
  • The current study evaluated the potential of Pichia fermentans for manufacturing of xylitol from SCB and OP hydrolysates through co- fermentation strategy. (bvsalud.org)
  • This method for the first time describes reliable experimental procedures for efficient inducible methanol-free recombinant protein production in Pichia pastoris (Komagataella phaffii) , employing the carbon source regulated promoters P DC and P DF in small scale experiments while cultivation parameters can be monitored online, and a constant glycerol feed is applied. (jove.com)
  • Research and studies show that Pichia pastoris developed as a heterologous protein expression system through a recombinant protein expression technology. (bitforbes.com)
  • In the biotech industry, some studies find the best recombinant protein in Pichia pastoris. (bitforbes.com)
  • Pichia (Hansenula and Hyphopichia are obsolete synonyms) is a genus of yeasts in the family Pichiaceae with spherical, elliptical, or oblong acuminate cells. (wikipedia.org)
  • Some Pichia representatives can be found in raw milk and cheese, such as P. anomala (formerly named Hansenula anomala). (wikipedia.org)
  • The main advantage of this technique is that high level methanol free protein expression can be achieved using Pichia pastoris, which is supported by the exact online monitoring of important cultivation parameters. (jove.com)
  • 2005 Pichia norvegensis Leask & Yarrow 1976 Pichia occidentalis (Kurtzman, M.J. Smiley & C.J. Johnson) Kurtzman, Robnett & Bas. (wikipedia.org)
  • Pichia pastoris expressed over 200 heterologous proteins. (bitforbes.com)
  • The global objective of the project is the optimisation of the production process of heterologous proteins in \i pichia pastoris\i0 system. (uab.cat)
  • Both heterologous proteins are going to be compared with the nativeproteins or produced in other expression systems to validate the capacity of \i Pichia pastoris\i0 expression system from the point of view of biological activity of produced heterologous proteins. (uab.cat)
  • Comparison of the heterologous expression of Trichoderma reesei en-doglucanase II and cellobiohydrolase II in the yeasts Pichia pastoris and Yarrowia lipolytica . (ugm.ac.id)
  • The resultnig beech hydrolysate was used for cultivation of yeast Pichia stipitits at various pH (3,7, 4,4, 5,1) and temperature (25, 29, 33°C) conditions, as well as various aeration rates and media composition. (unizg.hr)
  • Biosynthesis of flavins and its regulation in the yeast Pichia guilliermondii]. (medscape.com)
  • In winemaking, some species of Pichia can create potential faults in wines. (wikipedia.org)
  • Some Pichia species (e.g. (wikipedia.org)
  • A Pichia pastoris is a host organism for the production of recombinant proteins. (bitforbes.com)
  • Can Pichia pastoris be a model organism? (bitforbes.com)
  • The Pichia pastoris is a good model organism. (bitforbes.com)
  • The Pichia pastoris works as a genetic model organism for gene analysis and crossing. (bitforbes.com)
  • Interestingly, you can find Pichia in raw milk and cheese that people consume every day. (bitforbes.com)
  • Several key factors are essential for successful fermentation of pichia pastoris. (infors-ht.com)
  • Characterization and high level expression of acidic en-doglucanase in Pichia pastoris . (ugm.ac.id)
  • What are the advantages of using Pichia pastoris for the protein expression system? (bitforbes.com)
  • Is Pichia Pastoris the Best Protein Expression System? (labiotech.eu)
  • The previous studies successfully expressed recombinant human insulin precursors (HIP) in Pichia pastoris truncated and full-length α-factor recombinant clones. (springeropen.com)
  • In this study, the recombinant EGII protein was expressed in Pichia pastoris and it has a molecular mass of approximately 52 kDa. (ugm.ac.id)
  • To achieve this global objective the project deals with genetic, physiology and metabolism aspects of \i Pichia pastoris\i0 system, culture broth selection, optimisation of fermetation process, downstream and purification, monitoring, mathematical modelling, control and scale-up. (uab.cat)
  • Pichia pastoris GS115;Pichia pastoris X-33;Human chorionic gonadotropin;Human follicle stimulating hormone;Glycosylation;Hypermannosylation;15N-Labeling. (iisc.ac.in)
  • One of which is the growing interest in Pichia pastoris for protein expression. (bitforbes.com)
  • Gene optimization and efficient expression of Trichoderma reesei Cel5A in Pichia pas-toris . (ugm.ac.id)