OrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentInformation Science
PichiaMethanolRecombinant ProteinsKiller Factors, YeastMolecular Sequence DataCloning, MolecularSaccharomycetales
Pichia
Yeast-like ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES isolated from exuded tree sap.
Methanol
A colorless, flammable liquid used in the manufacture of FORMALDEHYDE and ACETIC ACID, in chemical synthesis, antifreeze, and as a solvent. Ingestion of methanol is toxic and may cause blindness.
Recombinant Proteins
Proteins prepared by recombinant DNA technology.
Killer Factors, Yeast
Protein factors released from one species of YEAST that are selectively toxic to another species of yeast.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Cloning, Molecular
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Saccharomycetales
An order of fungi in the phylum Ascomycota that multiply by budding. They include the telomorphic ascomycetous yeasts which are found in a very wide range of habitats.

Kodamaea nitidulidarum, Candida restingae and Kodamaea anthophila, three new related yeast species from ephemeral flowers. (1/1453)

Three new yeast species were discovered during studies of yeasts associated with ephemeral flowers in Brazil, Australia and Hawaii. Their physiological and morphological similarity to Kodamaea (Pichia) ohmeri suggested a possible relationship to that species, which was confirmed by rDNA sequencing. Kodamaea nitidulidarum and Candida restingae were found in cactus flowers and associated nitidulid beetles in sand dune ecosystems (restinga) of South-eastern Brazil. Over 350 strains of Kodamaea anthophila were isolated from Hibiscus and morning glory flowers (Ipomoea spp.) in Australia, and from associated nitidulid beetles and Drosophila hibisci. A single isolate came from a beach morning glory in Hawaii. Expansion of the genus Kodamaea to three species modified the existing definition of the genus only slightly. The type and isotype strains are as follows: K. nitidulidarum strains UFMG96-272T (h+; CBS 8491T) and UFMG96-394I (h-; CBS 8492I); Candida restingae UFMG96-276T (CBS 8493T); K. anthophila strains UWO(PS)95-602.1T (h+; CBS 8494T), UWO(PS)91-893.2I (h-; CBS 8495I) and UWO(PS)95-725.1I (h-; CBS 8496I).  (+info)

Glycosylation of asparagine-28 of recombinant staphylokinase with high-mannose-type oligosaccharides results in a protein with highly attenuated plasminogen activator activity. (2/1453)

The properties of recombinant staphylokinase (SakSTAR) expressed in Pichia pastoris cells have been determined. The single consensus N-linked oligosaccharide linkage site in SakSTAR (at Asn28 of the mature protein) was occupied in approximately 50% of the expressed protein with high-mannose-type oligosaccharides. The majority of these glycans ranged in polymerization state from Man8GlcNAc2 to Man14GlcNAc2, with the predominant species being Man10GlcNAc2 and Man11GlcNAc2. Glycosylated SakSTAR (SakSTARg) did not differ from its aglycosyl form in its aggregation state in solution, its thermal denaturation properties, its ability to form a complex with human plasmin (hPm), the amidolytic properties of the respective SakSTAR-hPm complexes, or its ability to liberate the amino-terminal decapeptide required for formation of a functional SakSTAR-hPm plasminogen activator complex. However, this latter complex with SakSTARg showed a greatly reduced ability to activate human plasminogen (hPg) as compared with the same complex with the aglycosyl form of SakSTAR. We conclude that glycosylation at Asn28 does not affect the structural properties of SakSTAR or its ability to participate in the formation of an active enzymatic complex with hPm, but it is detrimental to the ability of the SakSTAR-hPm complex to serve as a hPg activator. This is likely due to restricted access of hPg to the active site of the SakSTARg-hPm complex.  (+info)

Protection of mice against a lethal influenza virus challenge after immunization with yeast-derived secreted influenza virus hemagglutinin. (3/1453)

The A/Victoria/3/75 (H3N2-subtype) hemagglutinin (HA) gene was engineered for expression in Pichia pastoris as a soluble secreted molecule. The HA cDNA lacking the C-terminal transmembrane anchor-coding sequence was fused to the Saccharomyces cerevisiae alpha-mating factor secretion signal and placed under control of the methanol-inducible P. pastoris alcohol oxidase 1 (AOX1) promoter. Growth of transformants on methanol-containing medium resulted in the secretion of recombinant non-cleaved soluble hemagglutinin (HA0s). Remarkably, the pH of the induction medium had an important effect on the expression level, the highest level being obtained at pH 8.0. The gel filtration profile and the reactivity against a panel of different HA-conformation specific monoclonal antibodies indicated that HA0s was monomeric. Analysis of the N-linked glycans revealed a typical P. pastoris type of glycosylation, consisting of glycans with 10-12 glycosyl residues. Mice immunized with purified soluble hemagglutinin (HA0s) showed complete protection against a challenge with 10 LD50 of mouse-adapted homologous virus (X47), whereas all control mice succumbed. Heterologous challenge with X31 virus [A/Aichi/2/68 (H3N2-subtype)], resulted in significantly higher survival rates in the immunized group compared with the control group. These results, together with the safety, reliability and economic potential of P. pastoris, as well as the flexibility and fast adaptation of the expression system may allow development of an effective recombinant influenza vaccine.  (+info)

Cloning, mutagenesis, and structural analysis of human pancreatic alpha-amylase expressed in Pichia pastoris. (4/1453)

Human pancreatic alpha-amylase (HPA) was expressed in the methylotrophic yeast Pichia pastoris and two mutants (D197A and D197N) of a completely conserved active site carboxylic acid were generated. All recombinant proteins were shown by electrospray ionization mass spectrometry (ESI-MS) to be glycosylated and the site of attachment was shown to be Asn461 by peptide mapping in conjunction with ESI-MS. Treatment of these proteins with endoglycosidase F demonstrated that they contained a single N-linked oligosaccharide and yielded a protein product with a single N-acetyl glucosamine (GlcNAc), which could be crystallized. Solution of the crystal structure to a resolution of 2.0 A confirmed the location of the glycosyl group as Asn461 and showed that the recombinant protein had essentially the same conformation as the native enzyme. The kinetic parameters of the glycosylated and deglycosylated wild-type proteins were the same while the k(cat)/Km values for D197A and D197N were 10(6)-10(7) times lower than the wild-type enzyme. The decreased k(cat)/Km values for the mutants confirm that D197 plays a crucial role in the hydrolytic activity of HPA, presumably as the catalytic nucleophile.  (+info)

The N-terminal CUB-epidermal growth factor module pair of human complement protease C1r binds Ca2+ with high affinity and mediates Ca2+-dependent interaction with C1s. (5/1453)

The Ca2+-dependent interaction between complement serine proteases C1r and C1s is mediated by their alpha regions, encompassing the major part of their N-terminal CUB-EGF-CUB (where EGF is epidermal growth factor) module array. In order to define the boundaries of the C1r domain(s) responsible for Ca2+ binding and Ca2+-dependent interaction with C1s and to assess the contribution of individual modules to these functions, the CUB, EGF, and CUB-EGF fragments were expressed in eucaryotic systems or synthesized chemically. Gel filtration studies, as well as measurements of intrinsic Tyr fluorescence, provided evidence that the CUB-EGF pair adopts a more compact conformation in the presence of Ca2+. Ca2+-dependent interaction of intact C1r with C1s was studied using surface plasmon resonance spectroscopy, yielding KD values of 10.9-29.7 nM. The C1r CUB-EGF pair bound immobilized C1s with a higher KD (1.5-1.8 microM), which decreased to 31.4 nM when CUB-EGF was used as the immobilized ligand and C1s was free. Half-maximal binding was obtained at comparable Ca2+ concentrations ranging from 5 microM with intact C1r to 10-16 microM for C1ralpha and CUB-EGF. The isolated CUB and EGF fragments or a CUB + EGF mixture did not bind C1s. These data demonstrate that the C1r CUB-EGF module pair (residues 1-175) is the minimal segment required for high affinity Ca2+ binding and Ca2+-dependent interaction with C1s and indicate that Ca2+ binding induces a more compact folding of the CUB-EGF pair.  (+info)

Cloning, expression, and properties of a nonneuronal secreted acetylcholinesterase from the parasitic nematode Nippostrongylus brasiliensis. (6/1453)

We have isolated a full-length cDNA encoding an acetylcholinesterase secreted by the nematode parasite Nippostrongylus brasiliensis. The predicted protein is truncated in comparison with acetylcholinesterases from other organisms such that the carboxyl terminus aligns closely to the end of the catalytic domain of the vertebrate enzymes. The residues in the catalytic triad are conserved, as are the six cysteines which form the three intramolecular disulfide bonds. Three of the fourteen aromatic residues which line the active site gorge in the Torpedo enzyme are substituted by nonaromatic residues, corresponding to Tyr-70 (Thr), Trp-279 (Asn), and Phe-288 (Met). High level expression was obtained via secretion from Pichia pastoris. The purified enzyme behaved as a monomeric hydrophilic species. Although of invertebrate origin and possessing the above substitutions in the active site gorge residues, the enzyme efficiently hydrolyzed acetylthiocholine and showed minimal activity against butyrylthiocholine. It displayed excess substrate inhibition with acetylthiocholine at concentrations over 2. 5 mM and was highly sensitive to both active site and "peripheral" site inhibitors. Northern blot analysis indicated a progressive increase in mRNA for AChE B in parasites isolated from 6 days postinfection.  (+info)

Positive selection of novel peroxisome biogenesis-defective mutants of the yeast Pichia pastoris. (7/1453)

We have developed two novel schemes for the direct selection of peroxisome-biogenesis-defective (pex) mutants of the methylotrophic yeast Pichia pastoris. Both schemes take advantage of our observation that methanol-induced pex mutants contain little or no alcohol oxidase (AOX) activity. AOX is a peroxisomal matrix enzyme that catalyzes the first step in the methanol-utilization pathway. One scheme utilizes allyl alcohol, a compound that is not toxic to cells but is oxidized by AOX to acrolein, a compound that is toxic. Exposure of mutagenized populations of AOX-induced cells to allyl alcohol selectively kills AOX-containing cells. However, pex mutants without AOX are able to grow. The second scheme utilizes a P. pastoris strain that is defective in formaldehyde dehydrogenase (FLD), a methanol pathway enzyme required to metabolize formaldehyde, the product of AOX. AOX-induced cells of fld1 strains are sensitive to methanol because of the accumulation of formaldehyde. However, fld1 pex mutants, with little active AOX, do not efficiently oxidize methanol to formaldehyde and therefore are not sensitive to methanol. Using these selections, new pex mutant alleles in previously identified PEX genes have been isolated along with mutants in three previously unidentified PEX groups.  (+info)

Physiological effects and adjuvanticity of recombinant brushtail possum TNF-alpha. (8/1453)

The present paper describes the physiological properties of recombinant possum TNF-alpha and an adjuvant effect on antibody responses to the model protein antigen, keyhole limpet haemocyanin (KLH). For these studies recombinant possum TNF-alpha was produced in the yeast Pichia pastoris. The recombinant cytokine was secreted into the culture medium and purified by gel filtration. Possum TNF-alpha produced in this expression system was N-glycosylated and bioactive in two different assays. In a murine fibroblast L929 cytotoxicity assay, the possum TNF-alpha had lower specific activity compared to human TNF-alpha, while in a possum-specific assay, possum TNF-alpha enhanced the proliferation of PHA-stimulated possum thymocytes and was more active than human TNF-alpha. The physiological effect of the recombinant possum TNF-alpha was investigated in groups of possums administered doses of 6, 30 or 150 micrograms of cytokine. For each dose, TNF-alpha caused profound effects on the numbers of circulating leucocytes characterized by a three-to-four-fold increase in neutrophil numbers at 6-24 h after injection and an initial sharp decrease in lymphocyte numbers. The efficacy of TNF-alpha as an immunological adjuvant was determined in possums administered KLH (125 micrograms) in an aqueous or Al(OH)3-based formulation with or without added recombinant TNF-alpha (150 micrograms). Serum antibody responses to KLH were monitored by ELISA. The TNF-alpha stimulated two-fold and four-fold increases in antibody levels in aqueous and Al(OH)3-based vaccine formulations, respectively. The strongest antibody responses were observed in the group of possums that received KLH formulated in Al(OH)3 with addition of TNF-alpha.  (+info)

High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation ( By Julia Cino PhD ...High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation ( By Julia Cino PhD ...
... By Julia Cino PhD ... Introduction Over th...The production of a functional protein is intimately related to the ce... Pichia pastoris Expression System /...,High,Yield,Protein,Production,from,Pichia,pastoris,Yeast:,A,Protocol,for,Benchtop,Fermentation,biological,advanced biology technology,biology laboratory technology,biology device technology,latest biology technology
http://bio-medicine.org/biology-technology/High-Yield-Protein-Production-from-Pichia-pastoris-Yeast-3A-A-Protocol-for-Benchtop-Fermentation-1536-1/
Improvement in the secretory expression of recombinant Candida rugosa lipase in Pichia pastoris<...Improvement in the secretory expression of recombinant Candida rugosa lipase in Pichia pastoris<...
TY - JOUR. T1 - Improvement in the secretory expression of recombinant Candida rugosa lipase in Pichia pastoris. AU - Kuo, Ting Chun. AU - Shaw, Jei Fu. AU - Lee, Guan Chiun. PY - 2015/12/1. Y1 - 2015/12/1. N2 - The yeast Candida rugosa can secrete a mixture of lipase isoenzymes (Lips), which have been widely applied in industry. Eight Lip genes (LIP1 to LIP8) have been identified and are expressed in Pichia pastoris. However, the expression level was not sufficient for economical industrial application. In this study, two combined processes of antibiotic selection and low-temperature culture efficiently elicited a high-level secretion of recombinant Lip2 in P. pastoris. The LIP2 gene copy number of the Pichia transformants was increased by sequential selections at gradually increasing Zeocin concentrations. After the first selection at 500 μg/mL of Zeocin, three clones (500-clones) with 2.4-fold to 5.8-fold improvement in Lip2 secretion were identified from 105 survival clones through lipase ...
https://scholar.lib.ntnu.edu.tw/en/publications/improvement-in-the-secretory-expression-of-recombinant-candida-ru
Expression of recombinant actin 5C from Drosophila in the methylotrophyc yeast Pichia pastoris<...Expression of recombinant actin 5C from Drosophila in the methylotrophyc yeast Pichia pastoris<...
TY - JOUR. T1 - Expression of recombinant actin 5C from Drosophila in the methylotrophyc yeast Pichia pastoris. AU - Nevzglyadova, O. V.. AU - Artemov, A. V.. AU - Zenin, V. V.. AU - Verkhusha, V. V.. AU - Shavlovsky, M. M.. AU - Povarova, O. I.. AU - Stepanenko, O. V.. AU - Kuznetsova, I. M.. AU - Turoverov, K. K.. PY - 2007/6/1. Y1 - 2007/6/1. N2 - A system of expression for the foreign actin gene in yeast cells Pichia pastoris has been developed. As a target protein, the Drosophila cytoplasmic actin 5C, which has 90% homology to the β-actin of higher eukaryotes, was used. In the present work, in order to develop conditions for biosynthesis of the target protein in yeast cells and a purification procedure for the recombinant protein, a GFP-actin fusion protein containing green fluorescent protein (GFP) as a fusion tag was expressed and purified. The size and survival of P. pastoris cells producing recombinant protein were characterized and shown to depend on the accumulation of recombinant ...
https://einstein.pure.elsevier.com/en/publications/expression-of-recombinant-actin-5c-from-drosophila-in-the-methylo-4
Pichia expression systemPichia expression system
Several months back, a couple of postings about the Invitrogen Pichia pastoris expression system appeared. A look at the archives failed to find any answers to the posts. Does anyone out there have any experience (good or bad) with this expression system? I will summarize and post if necessary. Thanks _____________________________________ Dean R. Appling Dept. of Chemistry & Biochemistry Univ. of Texas at Austin Austin, TX 78712-1167 (512) 471-5842 (voice) (512) 471-5849 (fax) appling at utbc01.cm.utexas.edu ...
http://www.bio.net/bionet/mm/yeast/1993-November/000507.html
Yeast lysates carrying the nucleoprotein from measles virus vaccine as a novel subunit vaccine platform to deliver Plasmodium...
Yeast cells represent an established bioreactor to produce recombinant proteins for subunit vaccine development. In addition, delivery of vaccine antigens directly within heat-inactivated yeast cells is attractive due to the adjuvancy provided by the yeast cell. In this study, Pichia pastoris yeast lysates carrying the nucleoprotein (N) from the measles vaccine virus were evaluated as a novel subunit vaccine platform to deliver the circumsporozoite surface antigen (CS) of Plasmodium. When expressed in Pichia pastoris yeast, the N protein auto-assembles into highly multimeric ribonucleoparticles (RNPs). The CS antigen from Plasmodium berghei (PbCS) was expressed in Pichia pastoris yeast in fusion with N, generating recombinant PbCS-carrying RNPs in the cytoplasm of yeast cells. When evaluated in mice after 3-5 weekly subcutaneous injections, yeast lysates containing N-PbCS RNPs elicited strong anti-PbCS humoral responses, which were PbCS-dose dependent and reached a plateau by the pre-challenge time
https://0-malariajournal-biomedcentral-com.brum.beds.ac.uk/articles/10.1186/s12936-017-1908-7
Yeast lysates carrying the nucleoprotein from measles virus vaccine as a novel subunit vaccine platform to deliver Plasmodium...
Yeast cells represent an established bioreactor to produce recombinant proteins for subunit vaccine development. In addition, delivery of vaccine antigens directly within heat-inactivated yeast cells is attractive due to the adjuvancy provided by the yeast cell. In this study, Pichia pastoris yeast lysates carrying the nucleoprotein (N) from the measles vaccine virus were evaluated as a novel subunit vaccine platform to deliver the circumsporozoite surface antigen (CS) of Plasmodium. When expressed in Pichia pastoris yeast, the N protein auto-assembles into highly multimeric ribonucleoparticles (RNPs). The CS antigen from Plasmodium berghei (PbCS) was expressed in Pichia pastoris yeast in fusion with N, generating recombinant PbCS-carrying RNPs in the cytoplasm of yeast cells. When evaluated in mice after 3-5 weekly subcutaneous injections, yeast lysates containing N-PbCS RNPs elicited strong anti-PbCS humoral responses, which were PbCS-dose dependent and reached a plateau by the pre-challenge time
https://0-malariajournal-biomedcentral-com.brum.beds.ac.uk/articles/10.1186/s12936-017-1908-7/figures/5
Obtain Full Support from Creative Biolabs for Glycoengineering Projects - Uttarakhand News TodayObtain Full Support from Creative Biolabs for Glycoengineering Projects - Uttarakhand News Today
To help the researchers and pharmaceutical partners with discovering the most effective therapeutic glycoprotein, Creative Biolabs provides solutions on glycosylation, the most widely applied posttranslational modification (PTM) approach to change protein function and measure the recombinant biopharmaceutical immunogenicity. Based upon the efficient glycoengineered expression platforms (glyco-engineered mammalian cell expression system, glyco-engineered pichia pastoris expression system, and glyco-engineered plant-based expression system), Creative Biolabs is fully competent to handle the requests of therapeutic glycoprotein development and glycoengineering of antibody/cell line.. Creative Biolabs has updated the generally applied mammalian cell expression with glyco-engineered Chinese hamster ovary (CHO) cells and glyco-engineered human embryonic kidney 293 (HEK293) cells for glycoprotein production, which can guarantee the natural folding and sugar chain composition of glycoprotein, and ...
http://www.uttarakhandnewstoday.net/story/82966/obtain-full-support-from-creative-biolabs-for-glycoengineering-projects.html
KRIBB: Search Results
Glycoengineering of the methylotrophic yeast Hansenula polymorpha for the production of glycoproteins with trimannosyl core N-glycan by blocking core oligosaccharide assembly / Doo-Byoung Oh; J S Park; Moo Woong Kim; Seon Ah Cheon; Eun Jung Kim; Hye Yun Moon; Oh Suk Kwon; Sang Ki Rhee; Hyun Ah Kang , 2008 ...
https://repository.kribb.re.kr/simple-search?from_item=201005/4448&query=%28%28keyword%3A%22biotechnology%22%29%29
Functional expression of the lactate permease Jen1p of Saccharomyces cerevisiae in Pichia pastoris | Biochemical JournalFunctional expression of the lactate permease Jen1p of Saccharomyces cerevisiae in Pichia pastoris | Biochemical Journal
In Saccharomyces cerevisiae the activity for the lactate-proton symporter is dependent on JEN1 gene expression. Pichia pastoris was transformed with an integrative plasmid containing the JEN1 gene. After 24 h of methanol induction, Northern and Western blotting analyses indicated the expression of JEN1 in the transformants. Lactate permease activity was obtained in P. pastoris cells with a Vmax of 2.1 nmol·s−1·mg of dry weight−1. Reconstitution of the lactate permease activity was achieved by fusing plasma membranes of P. pastoris methanol-induced cells with Escherichia coli liposomes containing cytochrome c oxidase, as proton-motive force. These assays in reconstituted heterologous P. pastoris membrane vesicles demonstrate that S. cerevisiae Jen1p is a functional lactate transporter. Moreover, a S. cerevisiae strain deleted in the JEN1 gene was transformed with a centromeric plasmid containing JEN1 under the control of the glyceraldehyde-3-phosphate dehydrogenase constitutive promotor. ...
http://www.biochemj.org/content/376/3/781
Improved production of human type II procollagen in the yeast Pichia pastoris in shake flasks by a wireless-controlled fed...Improved production of human type II procollagen in the yeast Pichia pastoris in shake flasks by a wireless-controlled fed...
Here we describe a new technical solution for optimization of Pichia pastoris shake flask cultures with the example of production of stable human type II collagen. Production of recombinant proteins in P. pastoris is usually performed by controlling gene expression with the strong AOX1 promoter, which is induced by addition of methanol. Optimization of processes using the AOX1 promoter in P. pastoris is generally done in bioreactors by fed-batch fermentation with a controlled continuous addition of methanol for avoiding methanol toxification and carbon/energy starvation. The development of feeding protocols and the study of AOX1-controlled recombinant protein production have been largely made in shake flasks, although shake flasks have very limited possibilities for measurement and control. By applying on-line pO2 monitoring we demonstrate that the widely used pulse feeding of methanol results in long phases of methanol exhaustion and consequently low expression of AOX1 controlled genes. Furthermore, we
https://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-8-33
Construction of a Pichia pastoris strain efficiently producing recombinant human granulocyte-colony stimulating factor (rhG-CSF...Construction of a Pichia pastoris strain efficiently producing recombinant human granulocyte-colony stimulating factor (rhG-CSF...
Non-glycosylated, recombinant human granulocyte colony-stimulating factor (rhG-CSF), produced by Escherichia coli(filgrastim, leukostim) is widely used to treat a number of serious human diseases...
https://link.springer.com/article/10.1007%2Fs11033-019-05169-9
Adaptation of Hansenula polymorpha to methanol: a transcriptome analysis | BMC Genomics | Full TextAdaptation of Hansenula polymorpha to methanol: a transcriptome analysis | BMC Genomics | Full Text
Methylotrophic yeast species (e.g. Hansenula polymorpha, Pichia pastoris) can grow on methanol as sole source of carbon and energy. These organisms are important cell factories for the production of recombinant proteins, but are also used in fundamental research as model organisms to study peroxisome biology. During exponential growth on glucose, cells of H. polymorpha typically contain a single, small peroxisome that is redundant for growth while on methanol multiple, enlarged peroxisomes are present. These organelles are crucial to support growth on methanol, as they contain key enzymes of methanol metabolism. In this study, changes in the transcriptional profiles during adaptation of H. polymorpha cells from glucose- to methanol-containing media were investigated using DNA-microarray analyses. Two hours after the shift of cells from glucose to methanol nearly 20% (1184 genes) of the approximately 6000 annotated H. polymorpha genes were significantly upregulated with at least a two-fold differential
https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-11-1
Recombinant protein production in yeast : methods and protocols - Rensselaer Libraries
Optimising yeast as a host for recombinant protein production (review) / Nicklas Bonander and Roslyn M. Bill -- Which yeast species shall I choose? : Saccharomyces cerevisiae versus Pichia pastoris (review) / Richard A.J. Darby [and others] -- Preparation of Pichia pastoris expression plasmids / Christel Logez [and others] -- Preparation of Saccharomyces cerevisiae expression plasmids / David Drew and Hyun Kim -- Codon optimisation for heterologous gene expression in yeast / Kristina Hedfalk -- Yeast transformation to generate high-yielding clones / Mohammed Jamshad and Richard A.J. Darby -- Screening for high-yielding Pichia pastoris clones : the production of G protein-coupled receptors as a case study / Shweta Singh [and others] -- Screening for high-yielding Saccharomyces cerevisiae clones: using a green fluorescent protein fusion strategy in the production of membrane proteins / David Drew and Hyun Kim -- The effect of antifoam addition on protein production yields / Sarah J. Routledge and ...
http://link.lib.rpi.edu/portal/Recombinant-protein-production-in-yeast--methods/2P7ivY6mY7s/
中国科学院大连化学物理研究所机构知识库(DICP OpenIR): SECRETORY EXPRESSION OF THE EXO-INULINASE FROM K. MARXIANUS CBS 6556 IN PICHIA PASTORIS
Inulin is linear oligosaccharides [1]. Although inulin can be hydrolyzed to monosaccharides non-enzymatically by acid treatment, inhibitory by-products produced that complicate downstream biological process. Therefore, it is pivotal to establish a cost-effective inulinase (EC 3.2.1.7) production. In this study, we isolated the DNA sequence encoding the mature peptide sequence of the exo-inulinase gene from Kluyveromyces marxianus CBS 6556 and expressed in Pichia pastoris X-33. The purified recombinant enzyme (reINU) gave a specific activity of 13100 U•mg−1, which was about 100-fold higher that reported for the wild type protein isolated from K. marxianus CBS6556 [2, 3]. Ferguson plot analysis showed that secretion expressed reINU was a 169 kDa dimer. Detailed analytical assays showed that the optimal temperature and pH for reINU were 60 oC and pH 4.0, respectively. It was found that hydrolysis activity was about 20% higher in the presence of Mn2+. reINU was stable for over 3 days at room ...
http://cas-ir.dicp.ac.cn/handle/321008/113172
IJMS | Free Full-Text | Cloning and Expression of Aspergillus tamarii FS132 Lipase Gene in Pichia pastorisIJMS | Free Full-Text | Cloning and Expression of Aspergillus tamarii FS132 Lipase Gene in Pichia pastoris
A lipase gene (atl) was cloned from Aspergillustamarii FS132 for the first time. The gene was found to have an open reading frame of 1024 base pairs (bp), and the coding region of the gene contained two introns (51 bp and 52 bp). Multi-alignment analysis of the deduced amino acid sequence indicated high homology between the enzyme and mono- and diacylglycerol lipases from fungi Aspergillus. The recombinant lipase was expressed in Pichia pastoris GS115 cells. The recombinant lipase was found to have a molecular mass of 36.7 kDa, and it exhibited lipase activity of 20 U/mL in culture supernatant when tributyrin was used as the substrate.
http://www.mdpi.com/1422-0067/11/6/2373
Biotech Solutions
VTU Technology to offer unique combination of technologies for the high-level production of proteins with human-like glycosylation using Pichia GlycoSwitch®. Tucson, Arizona and Grambach, Austria, December 03, 2014 - /EPR BIOTECH NEWS/ - VTU Technology and Research Corporation Technologies (RCT) announced today that they have entered into a development and commercialization agreement for VTU to combine both companies´ Pichia pastoris protein production technologies. Pichia GlycoSwitch® - a new expression system for the production of glycoproteins with human-like glycosylation patterns - and VTU´s yield enhancing Pichia pastoris expression platform are now available in one package from VTU. This unique combination of technologies acting synergistically is of great benefit for customers as it leads to a high performance production platform for recombinant glycoproteins with superior product yields and uniform Man5 - or other human-like glycoforms.. Under the terms of the agreement, RCT grants ...
https://www.eprbiotechnews.com/category/biotech-solutions/
2013 - EQUIPMENT BOKU VIENNA INSTITUTE OF BIOTECHNOLOGY GMBH2013 - EQUIPMENT BOKU VIENNA INSTITUTE OF BIOTECHNOLOGY GMBH
Jadhav, V., Hackl, M., Druz, A., Shridhar, S., Chung, C-Y., Heffner, K.M., Kreil, D.P., Betenbaugh, M., Joseph Shiloach, J., Niall Barron, N., Grillari, J., Borth, N. (2013) CHO microRNA engineering is growing up: Recent successes and future challenges. Biotechn. Advances, 31:8:1501-1513. Jadhav, V., Hackl, M., Klanert, G., Hernandez Bort, J.A., Kunert, R., Borth, N., Grillari, J. (2014) Stable overexpression of miR-17 enhances recombinant protein production of CHO cells. J Biotechn 175, 38-44. Klug, L., Tarazona, P., Gruber, C., Grillitsch, K., Gasser, B., Trötzmüller, M., Köfeler, H., Leitner, E., Feussner, I., Mattanovich, D., Altmann, F., Daum, G., (2013) The lipidome and proteome of microsomes from the methylotrophic yeast Pichia pastoris, Biochimica et Biophysica Acta, 215-226. Klavins, K., Neubauer, S., Al Chalabi, A., Sonntag, D., Haberhauer-Troyer, C., Russmayer, H., Sauer, M., Mattanovich, D., Hann, S., Koellensperger, G. (2013) Interlaboratory comparison for quantitative primary ...
http://eq-vibt.boku.ac.at/publication/2013-2/
Construction of a novel MK-4 biosynthetic pathway in Pichia pastoris through heterologous expression of Hs UBIAD1 | Microbial...Construction of a novel MK-4 biosynthetic pathway in Pichia pastoris through heterologous expression of Hs UBIAD1 | Microbial...
With a variety of physiological and pharmacological functions, menaquinone is an essential prenylated product that can be endogenously converted from phylloquinone (VK1) or menadione (VK3) via the expression of Homo sapiens UBIAD1 (HsUBIAD1). The methylotrophic yeast, Pichia pastoris, is an attractive expression system that has been successfully applied to the efficient expression of heterologous proteins. However, the menaquinone biosynthetic pathway has not been discovered in P. pastoris. Firstly, we constructed a novel synthetic pathway in P. pastoris for the production of menaquinone-4 (MK-4) via heterologous expression of HsUBIAD1. Then, the glyceraldehyde-3-phosphate dehydrogenase constitutive promoter (PGAP) appeared to be mostsuitable for the expression of HsUBIAD1 for various reasons. By optimizing the expression conditions of HsUBIAD1, its yield increased by 4.37 times after incubation at pH 7.0 and 24 °C for 36 h, when compared with that under the initial conditions. We found HsUBIAD1
https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-019-1215-9
Pichia pastoris recombinant protein expression platform | Open-iPichia pastoris recombinant protein expression platform | Open-i
Pichia pastoris recombinant protein expression platform. Automated purification by affinity chromatography on Ni-NTA and enzymatic activities assays of His6-tag
https://openi.nlm.nih.gov/detailedresult.php?img=PMC4585289_fmicb-06-01002-g005&req=4
GlycoSwitch® : Expression Platform for Protein Production: Glyco Switch | Pichia Pastoris Protein Expression Platform :...GlycoSwitch® : Expression Platform for Protein Production: Glyco Switch | Pichia Pastoris Protein Expression Platform :...
Buy Pichia GlycoSwitch® : a Pichia Expression Platform enabling the production of proteins with controlled, human-like glycosylation.
https://pichia.com/try-pichia/try-pichia-glycoswitch/order-form/
PPIC Statewide Survey: Californians and Their Government - Public Policy Institute of CaliforniaPPIC Statewide Survey: Californians and Their Government - Public Policy Institute of California
The PPIC Statewide Survey delivers objective, advocacy-free information on the perceptions, opinions, and public policy preferences of California residents. PPIC invites input, comments, and suggestions from policy and public opinion experts and from its own advisory committee, but survey methods, questions, and content are determined solely by the PPIC survey team. The PPIC Statewide Survey relies on a rigorous survey methodology and is a charter member of the American Association for Public Opinion Research Transparency Initiative. The survey is conducted regularly throughout the year in the key areas of government, the environment, K-12 education, and higher education ...
http://www.ppic.org/publication/ppic-statewide-survey-californians-and-their-government-december-2017/
Orman | UH Department of Chemical and Biomolecular Engineering
Çalık P, Bozkurt B, Zerze GH, İnankur B, Bayraktar E, Boy E, Orman MA, Açık E and Özdamar, TH., Effect of co-substrate sorbitol different feeding strategies on human growth hormone production by recombinant Pichia pastoris. J. Chem. Technol. Biotechnol., 88: 1631-1640. , ...
http://www.chee.uh.edu/faculty/orman
Biomarkers allow detection of nutrient limitations and respective supplementation for elimination in Pichia pastoris fed-batch...Biomarkers allow detection of nutrient limitations and respective supplementation for elimination in Pichia pastoris fed-batch...
Production of heterologous proteins in yeast becomes more and more important in the biotechnological sector for research use, therapeutics or sustainable energy sources [1, 2]. This development has led to the constant demand for improvement of hosts and processes for recombinant protein production (RPP). Common approaches for improvement of RPP are mostly based on strain engineering [3], process optimization [4] including adaptation of culture conditions [5-8], feed strategies [9], or media optimization [10, 11]. As production processes become more and more specific due to special needs of production hosts [11] and the recombinant product, media need to be developed for a specific process and are often tailor-made [12]. In general, cultivation media should be chemically defined, however, high level protein synthesis, influencing cell physiology, might make supplementation with e.g. amino acids necessary [13]. For bioreactor cultivations of the yeast production host Pichia pastoris (Komagataella ...
https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-017-0730-9
Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris.
ABSTRACT: BACKGROUND: Inducible high-level expression is favoured for recombinant protein production in Pichia pastoris. Therefore, novel regulated promoters are desired, ideally repressing heterologous gene expression during initial growth and enabl
http://www.biomedsearch.com/nih/Induction-without-methanol-novel-regulated/23347568.html
Altmetric - Improved microscale cultivation of Pichia pastoris for clonal screeningAltmetric - Improved microscale cultivation of Pichia pastoris for clonal screening
Expanding the application of technical enzymes, e.g., in industry and agriculture, commands the acceleration and cost-reduction of bioprocess development. Microplates and shake flasks are massively employed during screenings and early phases of bioprocess development, although major drawbacks such as low oxygen transfer rates are well documented. In recent years, miniaturization and parallelization of stirred and shaken bioreactor concepts have led to the development of novel microbioreactor concepts. They combine high cultivation throughput with reproducibility and scalability, and represent promising tools for bioprocess development. Parallelized microplate cultivation of the eukaryotic protein production host Pichia pastoris was applied effectively to support miniaturized phenotyping of clonal libraries in batch as well as fed-batch mode. By tailoring a chemically defined growth medium, we show that growth conditions are scalable from microliter to 0.8 L lab-scale bioreactor batch cultivation ...
https://www.altmetric.com/details/40663113
Recombinant proteinase 3 (Wegeners antigen) expressed in Pichia pastoris is functionally active and is recognized by patient...Recombinant proteinase 3 (Wegeners antigen) expressed in Pichia pastoris is functionally active and is recognized by patient...
HARMSEN, M. C., HEERINGA, P., VAN DER GELD, Y. M., HUITEMA, M. G., KLIMP, A., TIRAN, A. and KALLENBERG, C. G. M. (1997), Recombinant proteinase 3 (Wegeners antigen) expressed in Pichia pastoris is functionally active and is recognized by patient sera. Clinical & Experimental Immunology, 110: 257-264. doi: 10.1111/j.1365-2249.1997.tb08325.x ...
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2249.1997.tb08325.x/references
covid-19 vaccine and ajovy Archives - Headache and Facial Pain Symptom Checkercovid-19 vaccine and ajovy Archives - Headache and Facial Pain Symptom Checker
Vyepti was the 4th and most recent of the CGRP mAbs to become available. It is made by Lundbeck and was FDA approved for migraine prevention 2/21/20. The antibody is produced in Pichia pastoris yeast cells by recombinant DNA technology. It targets the CGRP ligand, rather than the CGRP receptor. It binds very strongly to the CGRP ligand, interfering with its ability to bind to the CGRP receptor and activate the migraine. It comes in 100 mg and 300 mg doses and is dosed once quarterly (every 3 months) by a quick 30-minute infusion. The 100 mg dose is the recommended starting dose which can be titrated as needed to the higher dose later.. This is the only intravenous (IV) option available. Since it is administered IV, it is 100% bioavailable compared to the bioavailability of the other subcutaneous injections of 50-82%. It also reaches Cmax (maximum concentration) in about 30 minutes compared to 5-7 days of the other subcutaneous injections. Therefore, not surprisingly Vyepti showed treatment ...
https://virtualheadachespecialist.com/tag/covid-19-vaccine-and-ajovy/
First-ever vegan registered collagenFirst-ever vegan registered collagen
Derived from Pichia Pastoris yeast, the patented fermentation technology produces a non-allergenic, high-performance Vegan registered collagen
https://www.cosmeticsbusiness.com/news/article_page/First-ever_vegan_registered_collagen/169717
Genetic Evidence for the Role of the Vacuole in Supplying Secretory Organelles with Ca2 in Hansenula polymorpha - pdf descargar
Genetic Evidence for the Role of the Vacuole in Supplying Secretory Organelles with Ca2 in Hansenula polymorpha. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
http://libros.duhnnae.com/2017/jun9/149852364184-Genetic-Evidence-for-the-Role-of-the-Vacuole-in-Supplying-Secretory-Organelles-with-Ca2-in-Hansenula-polymorpha.php
Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris | BMC Biotechnology | Full TextIncreasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris | BMC Biotechnology | Full Text
The methylotrophic yeast P. pastoris has, during the last decades, increased in popularity as a eukaryotic host for recombinant protein expression [7]. Numerous reports have described successful overexpression of soluble proteins as well as of membrane proteins in this system, but sufficient protein yields have not always been obtained. Several methods have been described that could be used to improve the outcome of expression trials, among which an optimisation of recombinant gene dosage has proven to be one of the most potent ones [21]. When it comes to the aquaporin family of membrane proteins, optimisation of the nucleotide sequence both regarding codon composition and AT content [38] and controlled growth in bioreactors [36] have been reported to improve the yields of heterologous protein. However, in no reports has a systematic examination of the effect of gene dosage on recombinant aquaporin expression in P. pastoris been done and results obtained for other membrane proteins [12, 15, 33] ...
https://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-11-47
Plus itPlus it
Advanced tumor growth is angiogenesis dependent (27) and endothelial cell organization into vascular networks is a hallmark of the angiogenic process (28). Such organization involves the mobilization of endothelial cells from a quiescent state to form a quasi-mature vasculature that can supply the growing mass with nutrients and oxygen (28). Inhibition of endothelial cell tube formation by endostatin may be due (at least in part) to blockade of the endothelial cells mobilization stimuli, although it is unclear whether this inhibition requires direct interaction with the endothelial cell or other cells that provide the required stimulatory cytokines. Surprisingly, endostatin obtained from EntreMed or Calbiochem had different efficacy profiles in our endothelial cell tube formation assays. This observation was unexpected given that EntreMed produces human endostatin in a P. pastoris expression system and supplies it to Calbiochem for packaging and sale. Follow-up experiments were conducted to ...
http://mct.aacrjournals.org/content/2/9/845
KGI | Faculty & Research | ResearchKGI | Faculty & Research | Research
Recombinant protein production lies at the core of the biotechnology revolution. Recombinant proteins such as humanized chimeric antibodies, human growth hormone, human insulin and a variety of industrial enzymes can been expressed in the yeast Pichia pastoris, a premier organism for eucaryotic protein expression. Pichia pastoris grows rapidly on inexpensive substrates to very high cell densities, and can produce biologically active foreign proteins of higher eukaryotic origin since it performs important posttranslational protein modifications, including proteolytic processing, disulfide bridge formation and glycosylation.. Regenerative medicine is aimed at using biological solutions to restore function to damaged or diseased biological systems. Work in this field could potentially yield viable tissues or organs for replacement, as well as therapies for a number of diseases. Stem cell therapies and gene therapy are among the most promising research avenues in regenerative medicine. Stem cells ...
http://www.kgi.edu/faculty-and-research/research
Ppic.org SEO Report to Get More Traffic - KontactrPpic.org SEO Report to Get More Traffic - Kontactr
SEO analysis of ppic.org with performance opportunities, semantic audit, page speed as well as traffic, layout and social data about ppic.org.
https://kontactr.com/website/site/ppic.org
UM Students RepositoryUM Students Repository
Fibroblast Growth Factor 21 (FGF21) is a novel target with potential anti diabetic properties that are useful for treatment of hyperglycemia, insulin resistance, hyperlipidemia and metabolic disease. Producing recombinant FGF21 by E. coli without using fusion proteins is time consuming and will produce low quantity products. In this study, to establish and test the efficiency of other expression methods, the complete fgf21 gene was constructed by overlapping PCR. The recombinant fgf21 genes were expressed successfully in E. coli (TB1) and in yeast (Pichia pastoris) under the control of maltose binding promoter and alcohol oxidase I promoter. The degree of success in terms of yield and functionality of the produced recombinant proteins in vivo were compared by using animal models. The result demonstrated that both expression systems can promote more soluble FGF21 levels, with less purification steps while preserving the bioactivity of the protein in vivo. The FGF21 produced in P. pastoris ...
http://studentsrepo.um.edu.my/8844/
ORBi: Browsing ORBiORBi: Browsing ORBi
in Journal of industrial microbiology & biotechnology (2016), 43(4), 517-23. High Pichia pastoris biomass density could be obtained using high co-feeding rate of methanol and sorbitol in a fed-batch or continuous culture, while further higher feeding rate finally leads to oxygen ... [more ▼]. High Pichia pastoris biomass density could be obtained using high co-feeding rate of methanol and sorbitol in a fed-batch or continuous culture, while further higher feeding rate finally leads to oxygen limitation in bioreactor. In the literature, there is lack of report about AOX1 promoter regulation with regard to dissolved oxygen level (DO). Therefore, in this work, chemostat cultures were performed to investigate the cell growth, metabolism and regulation of the AOX1 promoter (pAOX1) regarding co-feeding rate of optimized methanol/sorbitol mixture (methanol fraction 0.60 C-mol/C-mol) using a P. pastoris Mut+/pAOX1-lacZ strain. The oxygen transfer rates (OTR) in bioreactor were kept in the range of ...
http://orbi.ulg.ac.be/browse?type=journal&value=Journal+of+Industrial+Microbiology+%26+Biotechnology
The impact of oxygen on the transcriptome of recombinant S. cerevisiae and P. pastoris - a comparative analysis | BMC Genomics ...The impact of oxygen on the transcriptome of recombinant S. cerevisiae and P. pastoris - a comparative analysis | BMC Genomics ...
Saccharomyces cerevisiae and Pichia pastoris are two of the most relevant microbial eukaryotic platforms for the production of recombinant proteins. Their known genome sequences enabled several transcriptomic profiling studies under many different environmental conditions, thus mimicking not only perturbations and adaptations which occur in their natural surroundings, but also in industrial processes. Notably, the majority of such transcriptome analyses were performed using non-engineered strains. In this comparative study, the gene expression profiles of S. cerevisiae and P. pastoris, a Crabtree positive and Crabtree negative yeast, respectively, were analyzed for three different oxygenation conditions (normoxic, oxygen-limited and hypoxic) under recombinant protein producing conditions in chemostat cultivations. The major differences in the transcriptomes of S. cerevisiae and P. pastoris were observed between hypoxic and normoxic conditions, where the availability of oxygen strongly affected
https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-12-218/figures/4
O-linked glycosylationO-linked glycosylation
I have a recombinant protein produced in the yeast Pichia pastoris that is partially glycosylated with O-linked sugar (it must be O-linked - there are no N-linked sites). I wish to remove the glycosylated protein from the non-glycosylated. I have tried using ConA-Sepharose (Pharmacia) and Glycine-Max (Sigma). Neither of these has appeared to bind. The ConA comes with instructions that have been followed. No instructions could be found for Gycine-MAx and Sigma were not forthcoming. Has anyone has experience with trying to purify O-linked sugars, with either of the above lectins, or using any other reagents, I would very much like to hear from you. Please reply to me by e-mail as I do not regularly read this newsgroup. Tim -- Tim Dudgeon British Biotech Phone: 0865 748747 FAX: 0865 717598 email: dudgeon at britbio.co.uk ...
http://www.bio.net/bionet/mm/proteins/1995-March/002330.html
Gentaur Molecular :Ray Biotech \ Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor, Pichia \ 228-10556-2Gentaur Molecular :Ray Biotech \ Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor, Pichia \ 228-10556-2
Gentaur molecular products has all kinds of products like :search , Ray Biotech \ Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor, Pichia \ 228-10556-2 for more molecular products just contact us
http://www.antibody-antibodies.com/product_det.php?id=1784215&supplier=search&name=Recombinant%20Human%20Granulocyte%20Macrophage_Colony%20Stimulating%20Factor,%20Pichia
AUT0-INDUCIBLE SODIUM PHOSPHATE SYMPORTER PROMOTER FROM PICHIA PASTORIS AND METHOD FOR PRODUCING RECOMBINANT PROTEIN USING IT -...AUT0-INDUCIBLE SODIUM PHOSPHATE SYMPORTER PROMOTER FROM PICHIA PASTORIS AND METHOD FOR PRODUCING RECOMBINANT PROTEIN USING IT -...
151732DNAPichia pastorisgene(1)..(732) 1atgagtttgg ttgcacttca tcaattcgac tacatctttg cgatcgccat gatgtttgca 60 ttcttggatg cattcaacat cggcgcaaat gatgtagcaa actccttttc ttcatcagtc 120 tcctcgagat gtctgaaata ctggcaagcg atgatcttag ctgccatcat ggaatttttg 180 ggtgccgtct tagctggtgc ccgtgtcact gacactatta gaaagagaat tatcaacgtt 240 cacgctttcg acgacgaccc agccatgctg atgttgacca tggccactgc cttggtcggt 300 tcttctgtct ggttgagtat tgccacttat gtcagagctc ctgtctccac cactcattcc 360 attgttggtg gtgtcattgg tgctggtatc gctgctaaag gtgctggtga gatccactgg 420 ggatggagtg gatttgccaa gattgttgct tcttggttca tcgctccatg tgttgctggt 480 ggattcgctt ccgtcctttt cctcttctgt aagttctcaa ttttggagag aaagcatgat 540 gccagaaatg cccttctgtt tgctccatgt atcgtcttct tgacttttgc tgtcctgaca 600 atgttgatcg tctggaaggg agctccaaac ttgaacttgg acgacctttc tactggtgct 660 accgtcggnt ctatcttcgg tgttgctggt gttgctacaa tcatttacat cacattcttt 720 ttccattctt ga 732 225DNAArtificial Sequenceprimer 2gaatacacac aagcatctat ttgag 25 326DNAArtificial Sequenceprimer 3ccactccatc ...
http://www.patentsencyclopedia.com/app/20100279345
RCSB PDB - 1B3E: HUMAN SERUM TRANSFERRIN, N-TERMINAL LOBE, EXPRESSED IN PICHIA PASTORIS Literature Report PageRCSB PDB - 1B3E: HUMAN SERUM TRANSFERRIN, N-TERMINAL LOBE, EXPRESSED IN PICHIA PASTORIS Literature Report Page
1B3E: X-ray crystallography and mass spectroscopy reveal that the N-lobe of human transferrin expressed in Pichia pastoris is folded correctly but is glycosylated on serine-32.
http://www.rcsb.org/pdb/explore/litView.do?structureId=1B3E
The effect of α-mating factor secretion signal mutations on recombinan by Geoff P. Lin-Cereghino, Carolyn Stark et al.The effect of α-mating factor secretion signal mutations on recombinan by Geoff P. Lin-Cereghino, Carolyn Stark et al.
The methylotrophic yeast, Pichia pastoris, has been genetically engineered to produce many heterologous proteins for industrial and research purposes. In order to secrete proteins for easier purification from the extracellular medium, the coding sequence of recombinant proteins is initially fused to the Saccharomyces cerevisiae α-mating factor secretion signal leader. Extensive site-directed mutagenesis of the prepro-region of the α-mating factor secretion signal sequence was performed in order to determine the effects of various deletions and substitutions on expression. Though some mutations clearly dampened protein expression, deletion of amino acids 57-70, corresponding to the predicted 3rd alpha helix of α-mating factor secretion signal, increased secretion of reporter proteins horseradish peroxidase and lipase at least 50% in small-scale cultures. These findings raise the possibility that the secretory efficiency of the leader can be further enhanced in the future.
https://scholarlycommons.pacific.edu/cop-facarticles/720/
DIGITAL.CSIC: The pCri system: A vector collection for recombinant protein expression and purificationDIGITAL.CSIC: The pCri system: A vector collection for recombinant protein expression and purification
A major bottleneck in structural, biochemical and biophysical studies of proteins is the need for large amounts of pure homogenous material, which is generally obtained by recombinant overexpression. Here we introduce a vector collection, the pCri System, for cytoplasmic and periplasmic/extracellular expression of heterologous proteins that allows the simultaneous assessment of prokaryotic and eukaryotic host cells (Escherichia coli, Bacillus subtilis, and Pichia pastoris). By using a single polymerase chain reaction product, genes of interest can be directionally cloned in all vectors within four different rare restriction sites at the 5′end and multiple cloning sites at the 3′end. In this way, a number of different fusion tags but also signal peptides can be incorporated at the N-and C-terminus of proteins, facilitating their expression, solubility and subsequent detection and purification. Fusion tags can be efficiently removed by treatment with site-specific peptidases, such as tobacco ...
https://digital.csic.es/handle/10261/124435
SmartFlow Technologies White Papers
P. pastoris and E. coli are commonly used hosts for recombinant product expression because of their ability to produce large quantities of recombinant protein quickly and economically. P. pastoris can express proteins with correct primary, secondary and tertiary structure and post-translational modifications such as glycolysation, proteolytic processing and disulfide bond formation. E. coli are readily transformed and can express large quantities of proteins quickly with correct primary structure in the soluble or insoluble forms. These attributes ensure that each host will remain central in recombinant protein expression of the future ...
http://smartflow-tech.com/whitepapers.html
IL 8 Human Pichia | ProSpecIL 8 Human Pichia | ProSpec
Interleukin-8 Human Recombinant produced in Yeast is a single, glycosylated polypeptide chain containing 77 amino acids and having a molecular mass of 8904 Dalton.
https://www.prospecbio.com/IL-8-77_Human_Pichia
Lonza Launches XS™ Pichia 2.0 Expression and Manufacturing Platform for Development of Next Generation TherapeuticsLonza Launches XS™ Pichia 2.0 Expression and Manufacturing Platform for Development of Next Generation Therapeutics
Technology Networks is an internationally recognised publisher that provides access to the latest scientific news, products, research, videos and posters.
https://www.technologynetworks.com/cancer-research/product-news/lonza-launches-xs-pichia-20-expression-and-manufacturing-platform-for-development-of-next-294301
Equine IL-8 - IBI ScientificEquine IL-8 - IBI Scientific
IBI recombinant proteins are expressed in Pichia pastoris (yeast) system which provides many advantages over E. coli systems. Yeast systems promote proper prote
https://www.ibisci.com/collections/equine-il-8
Recombinant human N-acetylgalactosamine-6-sulfate sulfatase (GALNS) produced in the methylotrophic yeast Pichia pastoris
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
http://pubmedcentralcanada.ca/pmcc/articles/PMC4932491/
On-line monitoring and control of methanol concentration in shake-flask cultures of Pichia pastoris | UBC ChemistryOn-line monitoring and control of methanol concentration in shake-flask cultures of Pichia pastoris | UBC Chemistry
The methylotrophic yeast Pichia pastoris can be used to express recombinant genes at high levels under the control of the methanol-inducible alcohol oxidase 1 (AOX1) promoter. Accurate regulation of the methanol concentration in P. pastoris cultures is necessary to maintain induction, while preventing accumulation of methanol to cytotoxic levels. We developed an inexpensive methanol sensor that uses a gas-permeable silicone rubber tube immersed in the culture medium and an organic solvent vapor detector. The sensor was used to monitor methanol concentration continuously throughout a fed-batch shake-flask culture of a P. pastoris clone producing the N-lobe of human transferrin. The sensor calibration was stable for the duration of the culture and the output signal accurately reflected the methanol concentration determined off-line by HPLC. A closed-loop control system utilizing this sensor was developed and used to maintain a 0.3% (v/v) methanol concentration in the culture. Use of this system ...
https://www.chem.ubc.ca/line-monitoring-and-control-methanol-concentration-shake-flask-cultures-pichia-pastoris
Adaptation fermentation of Pichia stipitis and combination detoxification on steam exploded lignocellulosic prehydrolyzateAdaptation fermentation of Pichia stipitis and combination detoxification on steam exploded lignocellulosic prehydrolyzate
Yeast Pichia stipitis CBS 5776 was developed through adaptation fermentation step by step in steam exploded corn stover prehydrolyzate because high concentration of weak acids and other inhibitors present in the prehydrolyzate could degrade the fermentability. However, the adaptability of Pichia stipitis CBS 5776 in the prehydrolyzate was so limited that steam strip-ping and overliming were applied to remove these inhibitors from it. Corn stover was steam exploded; the filtrate of steam exploded corn stover was hydrolyzed with dilute sulfuric acid, and then the acid hydrolyzate was detoxified and fermented by Pichia stipitis CBS 5776. Steam stripping could remove volatile com-pounds from the acid hydrolyzate and the fil-trate. At a steam stripping time of 120min, 81% acetic acid and 59% formic acid were removed from the acid hydrolyzate, 77% acetic acid and 45% formic acid were removed from the filtrate, while furfural was stripped off completely from the acid hydrolyzate and the filtrate. Overliming
https://scirp.org/journal/papercitationdetails.aspx?paperid=504&JournalID=69
Production of Δ<sup>1</sup>-tetrahydrocannabinolic acid by the biosynthetic enzyme secreted from transgenic Pichia...
TY - JOUR. T1 - Production of Δ1-tetrahydrocannabinolic acid by the biosynthetic enzyme secreted from transgenic Pichia pastoris. AU - Taura, Futoshi. AU - Dono, Emi. AU - Sirikantaramas, Supaart. AU - Yoshimura, Kohji. AU - Shoyama, Yukihiro. AU - Morimoto, Satoshi. PY - 2007/9/28. Y1 - 2007/9/28. N2 - Δ1-Tetrahydrocannabinolic acid (THCA) synthase is the enzyme that catalyzes the oxidative cyclization of cannabigerolic acid into THCA, the acidic precursor of Δ1-tetrahydrocannabinol. We developed a novel expression system for THCA synthase using a methylotrophic yeast Pichia pastoris as a host. Under optimized conditions, the transgenic P. pastoris secreted ∼1.32 nkat/l of THCA synthase activity, and the culture medium, from which the cells were removed, effectively synthesized THCA from cannabigerolic acid with a ∼98% conversion rate. The secreted THCA synthase was readily purified to homogeneity. Interestingly, endoglycosidase treatment afforded a deglycosylated THCA synthase with more ...
https://kyushu-u.pure.elsevier.com/ja/publications/production-of-%CE%B4sup1sup-tetrahydrocannabinolic-acid-by-the-biosynt
Codon optimization of Candida rugosa lip1 gene for improving expression in Pichia pastoris and biochemical characterization of...Codon optimization of Candida rugosa lip1 gene for improving expression in Pichia pastoris and biochemical characterization of...
TY - JOUR. T1 - Codon optimization of Candida rugosa lip1 gene for improving expression in Pichia pastoris and biochemical characterization of the purified recombinant LIP1 lipase. AU - Chang, Shu Wei. AU - Lee, Guan Chiun. AU - Shaw, Jei Fu. N1 - Copyright: Copyright 2011 Elsevier B.V., All rights reserved.. PY - 2006/2/8. Y1 - 2006/2/8. N2 - An important industrial enzyme, Candida rugosa lipase (CRL) possesses several different isoforms encoded by the lip gene family (lip1-lip7), in which the recombinant LIP1 is the major form of the CRL multigene family. Previously, 19 of the nonuniversal serine codons (CTG) of the lip1 gene hav been successfully converted into universal serine codons (TCT) by overlap extension PCR-based multiple-site-directed mutagenesis to express an active recombinant LIP1 in the yeast Pichia pastoris. To improve the expression efficiency of recombinant LIP1 in P. pastoris, a regional synthetic gene fragment of lip1 near the 5′ end of a transcript has been constructed to ...
https://scholar.lib.ntnu.edu.tw/en/publications/codon-optimization-of-candida-rugosa-lip1-gene-for-improving-expr
High-level extracellular production and characterization of Candida antarctica lipase B in Pichia pastoris. - Semantic ScholarHigh-level extracellular production and characterization of Candida antarctica lipase B in Pichia pastoris. - Semantic Scholar
The gene encoding lipase B from Candida antarctica (CalB) was expressed in Pichia pastoris after it was synthesized by the recursive PCR and cloned into the Pichia expression plasmid, pPICZαA. The CalB was successfully secreted in the recombinant P. pastoris strain X-33 with an apparent molecular weight of 34 kDa. For 140 h flask culture, the dry cell weight and the extracellular lipase activity reached at 5.4 g/l and 57.9 U/l toward p-nitrophenyl palmitate, respectively. When we performed the fed-batch fermentation using a methanol feeding strategy for 110 h, the dry cell weight and the extracellular lipase activity were increased to 135.7 g/l and 11,900 U/l; the CalB protein concentration was 1.18 g/l of culture supernatant. The characteristics of CalB recovered from the P. pastoris culture were compared with the commercial form of CalB produced in Aspergillus oryzae. The kinetic constants and specific activity, the effects of activity and stability on temperature and pH, the glycosylation extent,
https://www.semanticscholar.org/paper/High-level-extracellular-production-and-characteri-Eom-Lee/123b722ae50ecd28f572fb64f4155c768268d5d6
Production of medium-chain volatile flavour esters in Pichia pastoris whole-cell biocatalysts with extracellular expression of...Production of medium-chain volatile flavour esters in Pichia pastoris whole-cell biocatalysts with extracellular expression of...
Medium-chain volatile flavour esters are important molecules since they have extensive applications in food, fragrance, cosmetic, paint and coating industries, which determine different characteristics of aroma or taste in commercial products. Biosynthesis of these compounds by alcoholysis is catalyzed by acyl-CoA:ethanol O-acyltransferases Eht1 or Eeb1 in Saccharomyces cerevisiae. In this study, these two yeast enzymes were selected to explore their preparations as the form of whole cell biocatalysts for the production of volatile flavour esters. Here, the novel whole cell biocatalysts Pichia pastoris yeasts with functional extracellular expression of Eht1 or Eeb1 were constructed. Flavour production was established through an integrated process with coupled enzyme formation and ester biosynthesis in the recombinant yeasts in one pot, leading to the formation of volatile C6-C14 methyl and ethyl esters from wort medium. Interestingly, there is no significant difference between P. pastoris-EHT1 ...
http://eprints.nottingham.ac.uk/41901/
IJMS | Free Full-Text | Expression of an Endo-β-1,4-glucanase Gene from Orpinomyces PC-2 in Pichia pastoris | NotesIJMS | Free Full-Text | Expression of an Endo-β-1,4-glucanase Gene from Orpinomyces PC-2 in Pichia pastoris | Notes
The endo-β-1,4-glucanase gene celE from the anaerobic fungus Orpinomyces PC-2 was placed under the control of an alcohol oxidase promoter (AOX1) in the plasmid pPIC9K, and integrated into the genome of a methylotrophic yeast P. pastoris GS115 by electroporation. The strain with highest endo-β-1,4-glucanase activity was selected and designed as P. pastoris egE, and cultivated in shaking flasks. The culture supernatant was assayed by SDS-polyacrylamide gel electrophoresis and showed a single band at about 52 kDa. Furthermore, the recombinant P. pastoris egE was proved to possess the ability to utilize sodium carboxymethyl cellulose as a carbon source. The recombinant endoglucanase produced by P. pastoris showed maximum activity at pH 6.0 and temperature 45 °C, indicating it was a mesophilic neutral endo-β-1,4-glucanase, suitable for denim biofinishing/washing. Further research was carried out in suitable fermentation medium in shaking flasks. The most favorable methanol addition concentration was
http://www.mdpi.com/1422-0067/12/5/3366/notes
Metabolic Flux Analysis for Recombinant Protein Production by Pichia pastoris Using Dual Carbon Sources: Effects of Methanol...Metabolic Flux Analysis for Recombinant Protein Production by Pichia pastoris Using Dual Carbon Sources: Effects of Methanol...
The intracellular metabolic fluxes through the central carbon pathways in the bioprocess for recombinant human erythropoietin (rHuEPO) production by Pichia pastoris (Mut(+)) were calculated. to investigate the metabolic effects of dual carbon sources (methanol/sorbitol) and the methanol feed rate, and to obtain a deeper understanding the regulatory circuitry of P. pastoris, using the established stoichiometry-based model containing 102 metabolites and 141 reaction fluxes. Four fed-batch operations with (MS-) and without (M-) sorbitol were performed three different constant specific growth rates (h(-1)), and denoted as M-0.03, MS-0.02, MS-0.03, and MS-0.04. Considering the methanol consumption pathway, the M-0.03 and MS-0.02 conditions produced similar effects and had >85% of formaldehyde flux towards the assimilatory pathway. In contrast, the use of the dual carbon Source condition generated la shift in metabolism towards the dissimilatory pathway that corre- sponded to the shift in dilution ...
https://avesis.metu.edu.tr/yayin/3ff4cebe-0b5f-4083-840a-a7edaceec27d/metabolic-flux-analysis-for-recombinant-protein-production-by-pichia-pastoris-using-dual-carbon-sources-effects-of-methanol-feeding-rate
Optimization of five environmental factors to increase beta-propeller phytase production in Pichia pastoris and impact on the...Optimization of five environmental factors to increase beta-propeller phytase production in Pichia pastoris and impact on the...
Recently, we engineered Pichia pastoris Muts strains to produce several beta-propeller phytases, one from Bacillus subtilis and the others designed by a structure-guided consensus approach. Furthermore, we demonstrated the ability of P. pastoris to produce and secrete these phytases in an active form in shake-flask cultures. In the present work, we used a design of experiments strategy (Simplex optimization method) to optimize five environmental factors that define the culture conditions in the induction step to increase beta-propeller phytase production in P. pastoris bioreactor cultures. With the optimization process, up to 347,682 U (82,814 U/L or 6.4 g/L culture medium) of phytase at 68 h of induction was achieved. In addition, the impact of the optimization process on the physiological response of the host was evaluated. The results indicate that the increase in extracellular phytase production through the optimization process was correlated with an increase in metabolic activity of P. ...
http://onlinelibrary.wiley.com/doi/10.1002/btpr.1822/full?globalMessage=0
Cloning and constitutive expression of Deschampsia antarctica Cu/Zn superoxide dismutase in Pichia pastoris | BMC Research...Cloning and constitutive expression of Deschampsia antarctica Cu/Zn superoxide dismutase in Pichia pastoris | BMC Research...
Deschampsia antarctica shows tolerance to extreme environmental factors such as low temperature, high light intensity and an increasing UV radiation as result of the Antarctic ozone layer thinning. It is very likely that the survival of this species is due to the expression of genes that enable it to tolerate high levels of oxidative stress. On that account, we planned to clone the D. antarctica Cu/ZnSOD gene into Pichia pastoris and to characterize the heterologous protein. The Copper/Zinc superoxide dismutase (Cu/ZnSOD) gene, SOD gene, was isolated from a D. antarctica by cDNA library screening. This SOD gene was cloned in the expression vector pGAPZαA and successfully integrated into the genome of the yeast P. pastoris SMD1168H. A constitutive expression system for the expression of the recombinant SOD protein was used. The recombinant protein was secreted into the YPD culture medium as a glycosylated protein with a 32 mg/l expression yield. The purified recombinant protein possesses a specific
https://bmcresnotes.biomedcentral.com/articles/10.1186/1756-0500-2-207
strong|Evaluation of fermentation kinetics of xylose to ethanol fermentation in the presence of acetic acid by Pichia stipitis:...
Evaluation of fermentation kinetics of xylose to ethanol fermentation in the presence of acetic acid by Pichia stipitis: Modeling and experimental data comparison
http://op.niscair.res.in/index.php/IJCT/article/view/14640
Production of a Rhizopus oryzae lipase from Pichia pastoris using alternative operational strategiesProduction of a Rhizopus oryzae lipase from Pichia pastoris using alternative operational strategies
Different cultivation strategies have been compared for the production of Rhizopus oryzae lipase (ROL) from Pichia pastoris. Several drawbacks have been found using a methanol non-limited fed-batch. On the one hand, oxygen limitation appeared at early cell dry weights and, on the other hand, high cell death was observed. A temperature limited fed-batch has been proposed to solve both problems. However, in our case study a methanol non-limited fed-batch results in better productivities. Finally, a lower salt medium were used to overcome cell death problems and a temperature limited fed-batch was applied thereafter to solve oxygen transfer limitations. This combined strategy has resulted in lower productivities when compared to a methanol non-limited fed-batch. However the culture could be longer prolonged and a 1.3-fold purer final product was obtained mainly due to cell death reduction.. ...
http://kth.diva-portal.org/smash/record.jsf?pid=diva2:334846
Heterologous expression of isotypically labelled Trichoderma reesei tyrosinase 2 in Pichia pastoris<...Heterologous expression of isotypically labelled Trichoderma reesei tyrosinase 2 in Pichia pastoris<...
TY - CHAP. T1 - Heterologous expression of isotypically labelled Trichoderma reesei tyrosinase 2 in Pichia pastoris. AU - Westerholm-Parvinen, Ann. AU - Mattinen, Maija. AU - Selinheimo, Emilia. AU - Markku, Saloheimo. PY - 2007. Y1 - 2007. N2 - Tyrosinase (EC 1.14.18.1) is a copper-containing oxidase that is widely distributed in mammals, invertebrates, plants and microorganisms. In mammals the enzyme is essential for the formation of melanin pigments, whereas tyrosinases in fruit and vegetables are related to the browning reaction that occurs upon bruising and long term storage. Tyrosinase is of great interest for many applications in the field of medicine, biotechnology and food engineering. It is a promising target enzyme for prodrug activations in melanomas and in biotechnological applications including crosslinking of protein matrices. It is of great importance to find ligands and inhibitors for tyrosinase. Structural studies and screening for ligands and inhibitors can be carried out ...
https://cris.vtt.fi/en/publications/heterologous-expression-of-isotypically-labelled-trichoderma-rees
DISSERTATIONS.SE: Bench-Scale Production of Heterologous Proteins from Extremophiles- Escherichia coli and Pichia pastoris...
Search and download thousands of Swedish university dissertations (essays). Full text. Free. Dissertation: Bench-Scale Production of Heterologous Proteins from Extremophiles- Escherichia coli and Pichia pastoris based expression systems.
https://www.dissertations.se/dissertation/058bdf8fa5/
Biochemical Conversion of Acid-Pretreated Water Hyacinth (Eichhornia Crassipes) to Alcohol Using Pichia Stipitis NCIM3497 on...Biochemical Conversion of Acid-Pretreated Water Hyacinth (Eichhornia Crassipes) to Alcohol Using Pichia Stipitis NCIM3497 on...
Article Biochemical Conversion of Acid-Pretreated Water Hyacinth (Eichhornia Crassipes) to Alcohol Using Pichia Stipitis NCIM3497. Cleaning and removal of water hyacinth from lakes and various historical places government spends lacks of rupees per y...
https://www.environmental-expert.com/articles/biochemical-conversion-of-acid-pretreated-water-hyacinth-eichhornia-crassipes-to-alcohol-using-pichi-441817
SGDB | Show Synthetic Gene ID: 209SGDB | Show Synthetic Gene ID: 209
The dimorphic yeast Candida rugosa has an unusual codon usage that hampers the functional expression of genes derived from this yeast in a conventional heterologous host. Commercial samples of C. rugosa lipase (CRL) are widely used in industry, but contain several different isoforms encoded by the lip gene family, among which the isoform encoded by the gene lip1 is the most prominent. In a first laborious attempt, the lip1 gene was systematically modified by site-directed mutagenesis to gain functional expression in Saccharomyces cerevisiae. As alternative approach, the gene (1647 bp) was completely synthesized with an optimized nucleotide sequence in terms of heterologous expression in yeast and simplified genetic manipulation. The synthetic gene was functionally expressed in both hosts S. cerevisiae and Pichia pastoris, and the effect of heterologous leader sequences on expression and secretion was investigated. In particular, using P. pastoris cells, the synthetic gene was functionally ...
http://www.umbc.edu/codon/sgdb/show_records.php?rgid=209
Scheffersomyces stipitis (Pignal) Kurtzman et Suzuki ATCC ® 58785&Scheffersomyces stipitis (Pignal) Kurtzman et Suzuki ATCC ® 58785&
Scheffersomyces stipitis ATCC ® 58785™ Designation: CBS 6054 [CCRC 21777, IFO 10063, NRRL Y-11545] Application: Bioethanol production
https://www.atcc.org/products/all/58785.aspx
Scheffersomyces stipitis (Pignal) Kurtzman et Suzuki ATCC ® 58785DScheffersomyces stipitis (Pignal) Kurtzman et Suzuki ATCC ® 58785D
Scheffersomyces stipitis ATCC ® 58785D-2™ Designation: genomic DNA from CBS 6054 (ATCC 58785) Application: Bioethanol production
https://www.atcc.org/en/Products/Cells_and_Microorganisms/Fungi_and_Yeast/Fungal_and_Yeast_Genomic_DNA/58785D-2.aspx
YEAST PROMOTERS FROM PICHIA PASTORIS - Patent applicationYEAST PROMOTERS FROM PICHIA PASTORIS - Patent application
1311003DNAPichia pastoris 1tgccaggatc tgaccactac aagaagcgta attaagtatt tattatgtag cttgaggaat 60tgatttctaa aggccaaaga tgatgtagag caatagtaga gtgattagca agtttctgaa 120catgttttgt ggtcgggtat taggcgccgt acctctgtat tttttttttt tggcagatgg 180gatgaccact gtgcttacat aattttcgat gggccctttt ggaaacgacc actccttccg 240atcacgtaat actaggcgag tatcactacg cgtcggtatc cgcattttag ccgctttaac 300gacaaggtca gggtaaatac tgtgttgcat gggcgatgcc gtaggatctg gccaacttgg 360aacagagcag cccaccgcgt aggcagcgct tgggaccagt ttgttagcca atcagttccc 420ccgtctgagt ccctacacgc tcaggcttgc atggatatgc atgaaaaatg tcaactcagt 480cgcacacagg gatgctgttg tcgtggtgag aaaaagctaa aatagcaaac ccatagcaat 540cgagagactg tgattggaag caaaagaacc aggcattgac taggaaagac tgacaagaaa 600attagacggt gccagtggta tttcattgaa ttgcactgaa cacgcctccc atttccttcc 660ctcaatccat tcaaagggct aattaaacct tatctcggcc tacattagtt gtcatggaaa 720cgtctcactt tctctctgtg tcccacagta gcgagtaggc ggagagacca aaaatggtct 780ctcggcccta atcaaaacat cctttgtcgc gttgttttca gatcgattcg caatctgaga 840gaaaaacaat ...
http://www.patentsencyclopedia.com/app/20160097053
SDBN September 24th 2012 Event: Biotech and Beer | San Diego Biotechnology NetworkSDBN September 24th 2012 Event: Biotech and Beer | San Diego Biotechnology Network
Did you know that Louis Pasteur discovered microorganisms and developed his technique for killing them, pasteurization, while studying beer? He was also a master brewer and wrote a book on the subject. Beer and life science have a long history, indeed even enzymes were discovered by studying yeast fermentation.. In San Diego, we are lucky to be a hub for both biotech and beer, and weve found a very interesting intersection of the two in White Labs, a local company creating high quality yeast strains for breweries. The company was started by Chris White, who got a Ph.D. in Biochemistry at UCSD studying the yeast Pichia pastoris. Chris was also an avid home beer brewer and began to use his scientific expertise on brewers yeast. We will hear from him about how he used this experience to found White Labs, a creative entrepreneurial move that has led to biotech helping to develop San Diegos thriving beer industry.. Of course, weve been holding SDBN events at Green Flash Brewery for almost a year, ...
http://sdbn.org/2012/08/29/sdbn-september-24th-2012-event-biotech-and-beer/
دانلود مقاله Enhancement of oxygen transfer and recombinant hG-CSF production in Pichia pastoris fed-batch fermentation
سال انتشار: ۱۳۸۶ محل انتشار: پنجمین کنگره بین المللی مهندسی شیمی تعداد صفحات: ۸ نویسنده(ها): Bahrami - Biotechnology Group, Chemical Engineering Department,
http://transline.ir/enhancement-of-oxygen-transfer-and-recombinant-hg-csf-production-in-pichia-pastoris-fed-batch-fermentation/
Expression, Purification and Characterization of Human Recombinant Galectin 3 in Pichia pastorisExpression, Purification and Characterization of Human Recombinant Galectin 3 in Pichia pastoris
Background: Over the past century, the areas of genomics, proteomics and lipids have captured the attention of investigators worldwide. Carbohydrates, have recently received increased attention through the expanding field of glycobiology; probably because they are very complex and not encoded in the genome. Objectives: The purpose of this study was to express and purify recombinant human galectin 3via the Pichiapastoris expression system. Materials and Methods:cDNA of human galectin 3 gene was amplified with specific primers and cloned into a pcDNA3.1 vector with His-tag for easier purification using Ni2andchromatography. Furthermore, galectin 3was purified to homogeneity and confirmed using SDS-PAGE and western blotting. Results:The protein band corresponding to 29 kDa was excised from the gels, digested with trypsin and processed for mass spectrometric analysis by Matrix Assisted Laser Desorption/Ionization- Time of Flight Mass Spectroscopy (MALDI-TOF MS), using a Reflex III instrument.
http://www.ijbiotech.com/article_7257.html
ASMscience | Heterologous Protein Exp
Many filamentous fungi and yeast species have been used for heterologous protein. This chapter covers the most commonly used fungal expression systems (Saccharomyces cerevisiae, Pichia pastoris, and Aspergillus species), with a focus on vector systems, promoter and leader sequences, posttranslational modifications, and fermentation scalability. Similar to other heterologous protein expression systems, proteolytic hydrolysis often causes instability of foreign proteins in Pichia. In comparison with S. cerevisiae and P. pastoris, filamentous fungi are less studied for heterologous protein production with respect to their transcription and translation control, mRNA stability, glycosylation, and regulation of protein secretion. There are several web-accessible organizations such as the American Type Culture Collection (ATCC), the (Fungal Genetics Stock Center (FGSC), the Agricultural Research Service Culture Collection (NRRL), and the Belgium Culture Collection (BCCP) from which the necessary tools for
https://www.asmscience.org/content/book/10.1128/9781555816827.ch11
HOGENOM: PISTI1 1 PE967
ID PISTI1_1_PE967 STANDARD; PRT; 362 AA. AC PISTI1_1_PE967; A3GHJ2; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE Flags: Fragments; DE SubName: Full=Predicted protein;Flags: Fragment; (PISTI1_1.PE967). GN ORFNames=PICST_53517; OS SCHEFFERSOMYCES STIPITIS CBS 6054. OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Debaryomycetaceae; Scheffersomyces. OX NCBI_TaxID=322104; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS PISTI1_1.PE967. CC Pichia stipitis (strain CBS 6054 / NRRL Y-11545 / IFO 10063 / ATCC 5878 CC chromosome 1, complete sequence. CC -!- ANNOTATIONS ORIGIN:A3GHJ2_PICST CC -!- SIMILARITY: Contains 1 protein kinase domain. CC -!- GENE_FAMILY: HOG000233016 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; A3GHJ2; -. DR EMBL; AAVQ01000002; EAZ63064.2; -; Genomic_DNA. DR RefSeq; XP_001387087.2; XM_001387050.1. DR ...
http://pbil.univ-lyon1.fr/cgi-bin/acnuc-search-id?query=PISTI1_1_PE967&db=HOGENOM&ident=ACNUC26462
CiNii 論文 - Purification and Characterization of an α-Amylase of Pichia burtonii Isolated from...
Among more than 20 yeast strains isolated from the traditional starter murcha in Nepal, we characterized a yeast that might be involved in saccharification. This strain, identified as ,I,Pichia burtonii,/I,, produced an extracellular amylolytic enzyme when cultured in the presence of starch in the medium. Since no amylase secreted by ,I,P. burtonii,/I, has yet been reported, we purified the enzyme and determined its N-terminal amino acid sequence. Together with the results of a hydrolyzing activity assay toward various substrates, it was found to be an α-amylase. The purified enzyme, named ,I,Pichia burtonii,/I, α-amylase (PBA), was a glycoprotein with an apparent molecular mass of 51 kDa. Enzyme activity was optimal at pH 5.0 at 40 °C. The enzyme retained 80% of its original activity after incubation under the optimal pH condition at 50 °C for 30 min. The activity was inhibited by metal ions such as Cd,SUP,2+,/SUP,, Cu,SUP,2+,/SUP,, Hg,SUP,2+,/SUP,, Al,SUP,3+,/SUP,, and ...
https://ci.nii.ac.jp/naid/10018524123
HBsAg recombinant protein product blogHBsAg recombinant protein product blog
Blog on HBsAg recombinant protein product: The HBsAg n/a (Catalog #MBS434117) is a Recombinant Protein produced from Pichia Pastoris and is intended...
http://blog.mybiosource.com/hbsag-recombinant-protein_434117
Establishing a versatile fermentation and purification procedure for human proteins expressed in the yeasts Saccharomyces...Establishing a versatile fermentation and purification procedure for human proteins expressed in the yeasts Saccharomyces...
We describe the introduction of the yeasts Saccharomyces cerevisiae and Pichia pastoris as eukaryotic hosts for the routine production of recombinant proteins for a structural genomics initiative. We
https://link.springer.com/article/10.1023%2FB%3AJSFG.0000029207.13959.90
Pichia 2021Pichia 2021
Instructions will be provided on presentation format options for your talk or poster once selected for the program. We will try to be as flexible as possible. More than one submission per person is welcome. There is no limit to the number of posters; most any scientific presentation will be accepted. ...
https://www.eventinterface.com/pichia-2021/
Endostatin Protein Human Recombinant | KNO1 Antigen | ProSpecEndostatin Protein Human Recombinant | KNO1 Antigen | ProSpec
Endostatin Human Recombinant produced in Pichia Pastoris is a single, glycosylated, polypeptide having a total molecular mass of 20,000 Dalton C-terminal.
https://www.prospecbio.com/endostatin_human
Structural Insight into the Activation Mechanism of Human Pancreatic Prophospholipase A2Structural Insight into the Activation Mechanism of Human Pancreatic Prophospholipase A2
Abstract: Pancreatic phospholipase A2 (phospholipase A2 group 1B, G1B) belongs to the superfamily of secreted phospholipase A2 (PLA2) enzymes. G1B has been proposed to be a potential target for diseases such as hypertension, obesity, and diabetes. Human pancreatic prophospholipase A2 (pro-hG1B) is activated by cleavage of the first seven-residue propeptide (phospholipase A2 propeptide, PROP). However, questions still remain on the mode of action for pro-hG1B. In this work, we expressed pro-hG1B in Pichia pastoris and determined the crystal structure at 1.55-Å resolution. The x-ray structure demonstrates that pro-hG1B forms a trimer. In addition, PROP occupies the catalytic cavity and can be self-cleaved at 37 °C. A new membrane-bound surface and activation mechanism are proposed based on the trimeric model of pro-hG1B. We also propose a new autoproteolytic mechanism for pro-hG1B by the reaction triad Asp49-Arg0-Ser(-2) that is similar to the serine protease catalytic triad.. ...
https://scialert.net/asci/ascidetail.php?doi=jbche.2009.16659.16666&kw=
Purification of the Human α2 Isoform of Na,K-ATPase Expressed in Pichia pastoris. Stabilization by Lipids and FXYD1 ...Purification of the Human α2 Isoform of Na,K-ATPase Expressed in Pichia pastoris. Stabilization by Lipids and FXYD1 ...
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https://www.infona.pl/resource/bwmeta1.element.acs-doi-10_1021_bi701812c
for server - kelurahankemuning.info - 4
Click Generate. We now have two ranges, both with 3 models (no longer 4) available from 3. A Desktop search for windows server 2008 r2 with Plesk Panel control winvows brings flexibility and control to a new level of cost effective powerful website automation. CISSA is a non-profit, non-governmental organization of scientists and citizens combining rigorous scientific analysis, innovative policy development and effective citizen advocacy to build a cleaner, safer and healthier world. I may install two more software to handle email accounts if I feel dekstop in future. Do you wear glasses. We offer various types of Operating System, from Windows to Linux. In addition, the yeast PLD, Spo14p, is required to assemble sporulation-related prospore membranes under starvation conditions 62 and is involved in AP formation-dependent unconventional secretion of Pichia pastoris Acb1 63 Collectively, these results support the hypothesis that Asp connection string to sql server 2005 express is involved in ...
http://kelurahankemuning.info/for-server/index4.html
Alcohol Oxidase - AP20103AF-N | acris-antibodies.com
Alcohol Oxidase, 10 mg. Alcohol Oxidase (AOX) is a homooctameric flavoprotein consisting of eight identical subunits of ~74 kD, each containing a flavin adenine dinucleotide molecule (FAD) as a prosthetic group (van der Klei et al.
https://www.acris-antibodies.com/antibodies/primary-antibodies/alcohol-oxidase-ap20103af-n.htm
PPIC Statewide Survey: Californians and Education - Public Policy Institute of CaliforniaPPIC Statewide Survey: Californians and Education - Public Policy Institute of California
Half of Californians say the states budget situation is a big problem for K-12 education and six in 10 say state funding for local schools is not enough ...
https://www.ppic.org/publication/ppic-statewide-survey-californians-and-education-april-2015/
Pada Angusta Asana - Health - Lifestyle
Since this Asana is good for leading a life of chastity and continence, persons who are desirous of leading such a life may have suggest to themselve
http://www.exposeknowledge.com/kb/7613-pada-angusta-asana.aspx
CCCC 1987, Volume 52, Issue 12, Abstracts pp. 3024-3033, Cited by | Collection of Czechoslovak Chemical Communications
Steudel A., Nitzsche C.: The nad-dependent alcohol dehydrogenase of methylotrophic yeasts - an electrophoretic study (i). Acta Biotechnol. 1991, 11, 57. ,https://doi.org/10.1002/abio.370110117 ...
http://cccc.uochb.cas.cz/52/12/3024/cited_by/
Xylaria polymorpha, Dead Mans Fingers fungusXylaria polymorpha, Dead Mans Fingers fungus
Xylaria polymorpha, Dead Mans Fingers fungus: identification pictures (images), habitat, edible or poisonous; taxonomy, etymology, synonyms, similar species
https://www.first-nature.com/fungi/xylaria-polymorpha.php
Lonza and AdAlta Announce a Strain Development Contract for Novel Biologic Therapeutics - AdAlta - Developing i-body drugs to...Lonza and AdAlta Announce a Strain Development Contract for Novel Biologic Therapeutics - AdAlta - Developing i-body drugs to...
Basel, Switzerland and Melbourne, Australia, 18 Dec 2014 - Lonza and AdAlta announced today that they have entered into a strain development agreement for AdAltas alternative scaffold proteins, called i-bodies. Lonza will conduct strain development studies with the XS™ Pichia systems for several i-body molecules that AdAlta successfully expressed at research levels in their Melbourne-based laboratory. Strain development and manufacturing will be conducted by Lonza at its microbial facility in Visp, Switzerland to establish a commercially viable manufacturing process.. The XS™ Research Evaluation Agreement (REA) provided AdAlta scientists with access to Lonzas proprietary microbial expression systems, protocols and technical expertise. The licensable XS™ Technology Platform consists of multiple hosts and promoter systems that have been optimized for soluble expression driving simpler recovery and downstream processing, which saves development time and costs. After evaluating several ...
http://adalta.com.au/lonza-and-adalta-announce-a-strain-development-contract-for-novel-biologic-therapeutics/
PichiaPichia
T. Seki 2005 Pichia nakasei J.A. Barnett, R.W. Payne & Yarrow 1983 Pichia nanzhaoensis F.L. Hui & K.F. Liu 2018 Pichia ... Pichia Species Archived 2014-07-02 at the Wayback Machine, Doctor Fungus, url accessed 2014-02-27 Pichia at Dr Fungus Pichia in ... 2018 Pichia heedii Phaff, Starmer, M. Miranda & M.W. Mill. 1978 Pichia kluyveri Bedford ex Kudryavtsev 1960 Pichia megalospora ... M. Miranda 1976 Pichia sporocuriosa G. Péter, Tornai-Leh., Dlauchy & Vitányi 2000 Pichia terricola Van der Walt 1957 Pichia ...
https://en.wikipedia.org/wiki/Pichia
Pichia heediiPichia heedii
... is a species of yeast in the family Saccharomycetaceae. Described in 1978, it was found growing on a dead senita ... Pichia heedii in MycoBank. v t e (Articles with short description, Short description matches Wikidata, Articles with 'species' ... Phaff HJ, Starmer WT, Miranda M, Miller MW (1978). "Pichia heedii, a new species of yeast indigenous to necrotic cacti in the ...
https://en.wikipedia.org/wiki/Pichia_heedii
Pichia pastorisPichia pastoris
However Pichia has two main advantages over S. cerevisiae in laboratory and industrial settings: Pichia, as mentioned above, is ... The biggest advantage of Pichia over E. coli is that Pichia is capable of forming disulfide bonds and glycosylations in ... Higgins, D. R., & Cregg, J. M. (1998). "Introduction to Pichia pastoris". Pichia Protocols. 103: 1-15. doi:10.1385/0-89603-421- ... whereas Pichia cells have to be produced immediately before use. Expression yields in Pichia vary between different clones, so ...
https://en.wikipedia.org/wiki/Pichia_pastoris
Wickerhamomyces anomalusWickerhamomyces anomalus
Pichia anomala is a species of ascomycete and teleomorphic fungi of the genus Pichia. It is used as a preventive (biocontrol ... "Pichia anomala , Viticulture & Enology". wineserver.ucdavis.edu. Retrieved 2016-04-16. "Oxygen- and Glucose-Dependent ... Madrigal, T.; Maicas, S.; Mateo Tolosa, J. J. (2013-03-01). "Glucose and Ethanol Tolerant Enzymes Produced by Pichia ( ... Distinguished from some other species of Pichia by high osmotolerance, P. anomala ferments sucrose, and assimilates raffinose. ...
https://en.wikipedia.org/wiki/Wickerhamomyces_anomalus
Hospital-acquired infectionHospital-acquired infection
Su, Lin-Hui; Ou, Jonathan T.; Leu, Hsieh-Shong; Chiang, Ping-Cherng; Chiu, Yueh-Pi; Chia, Ju-Hsin; Kuo, An-Jing; Chiu, Cheng- ...
https://en.wikipedia.org/wiki/Hospital-acquired_infection
AlkaneAlkane
Some specialized yeasts, e.g., Candida tropicale, Pichia sp., Rhodotorula sp., can use alkanes as a source of carbon or energy ...
https://en.wikipedia.org/wiki/Alkane
Expression vectorExpression vector
A yeast commonly used for protein production is Pichia pastoris. Examples of yeast expression vector in Pichia are the pPIC ... "Pichia pastoris Expression System" (PDF). Invitrogen. "K. lactis Protein Expression Kit" (PDF). New England BioLabs Inc. Fields ... Cregg JM, Cereghino JL, Shi J, Higgins DR (2000). "Recombinant protein expression in Pichia pastoris". Molecular Biotechnology ...
https://en.wikipedia.org/wiki/Expression_vector
Protein productionProtein production
Expression systems using either S. cerevisiae or Pichia pastoris allow stable and lasting production of proteins that are ... Cregg JM, Cereghino JL, Shi J, Higgins DR (September 2000). "Recombinant protein expression in Pichia pastoris". Molecular ...
https://en.wikipedia.org/wiki/Protein_production
O Homem do Pau-BrasilO Homem do Pau-Brasil
Menotti Del Pichia Juliana Carneiro da Cunha...foreign dancer (allusion to Isadora Duncan) Dina Sfat...Branca Clara (allusion ...
https://en.wikipedia.org/wiki/O_Homem_do_Pau-Brasil
Ts8Ts8
In a study from Cordeiro et al (2022) they expressed recombinant Ts8 (rTs8) in Pichia pastoris yeast to be able to evaluate the ... This resulted in growth inhibition of Pichia pastoris. The native form of the toxin had a larger inhibitory effect than the ...
https://en.wikipedia.org/wiki/Ts8
Lysyl oxidaseLysyl oxidase
In the yeast strain Pichia pastoris, lysyl oxidase constitutes a homodimeric structure. Each monomer consists of an active site ... "The crystal structure of Pichia pastoris lysyl oxidase". Biochemistry. 42 (51): 15148-57. doi:10.1021/bi035338v. PMID 14690425 ...
https://en.wikipedia.org/wiki/Lysyl_oxidase
List of organisms named after famous people (born 1900-1949)List of organisms named after famous people (born 1900-1949)
nov., a new sibling species of Komagataella (Pichia) pastoris". Antonie van Leeuwenhoek. 111 (7): 1197-1207. doi:10.1007/s10482 ...
https://en.wikipedia.org/wiki/List_of_organisms_named_after_famous_people_(born_1900–1949)
CorbevaxCorbevax
The protein is produced by the yeast Pichia pastoris; the process is similar to that of existing Hepatitis B vaccines. In April ...
https://en.wikipedia.org/wiki/Corbevax
P. N. RangarajanP. N. Rangarajan
He leads a team of scientists engaged in the studies of Pichia pastoris, a methylotrophic yeast species, with regard to its ... "Transcriptional interference in the methylotrophic yeast, Pichia pastoris". IISER, Thiruvananthapuram. 2012. "BHD-2011" (PDF). ... Pichia pastoris at Indian Institute of Science Education and Research, Thiruvananthapuram in October 2012. He was the co- ... and pyruvate carboxylase-deficient conditions in Pichia pastoris". Microbiology. 157 (12): 3361-3369. doi:10.1099/mic.0.053488- ...
https://en.wikipedia.org/wiki/P._N._Rangarajan
WickerhamomycesWickerhamomyces
Yeasts belonging to the genera Candida, Pichia, and Wickerhamomyces have been found in Aedes and Anopheles genera of mosquitoes ... 2012 Some genera have been re-assigned from Pichia family; such as W. canadensis, W. ciferri, W. lynferdii, W. salvicola and W ...
https://en.wikipedia.org/wiki/Wickerhamomyces
Impossible FoodsImpossible Foods
"GRAS Notification for Soybean Leghemoglobin Protein Derived from Pichia pastoris" (PDF). Food and Drug Administration. Archived ...
https://en.wikipedia.org/wiki/Impossible_Foods
MidkineMidkine
Murasugi A, Kido I, Kumai H, Asami Y (2003). "Efficient production of recombinant human pleio-trophin in yeast, Pichia pastoris ... Pichia pastoris". Protein Expr. Purif. 27 (2): 244-52. doi:10.1016/S1046-5928(02)00587-9. PMID 12597883. ...
https://en.wikipedia.org/wiki/Midkine
PleiotrophinPleiotrophin
Murasugi A, Kido I, Kumai H, Asami Y (2003). "Efficient production of recombinant human pleio-trophin in yeast, Pichia pastoris ...
https://en.wikipedia.org/wiki/Pleiotrophin
Golgi apparatusGolgi apparatus
Pichia pastoris does have stacked Golgi, while Saccharomyces cerevisiae does not. In plants, the individual stacks of the Golgi ...
https://en.wikipedia.org/wiki/Golgi_apparatus
IL17AIL17A
Murphy KP, Gagne P, Pazmany C, Moody MD (March 1998). "Expression of human interleukin-17 in Pichia pastoris: purification and ...
https://en.wikipedia.org/wiki/IL17A
Golgi matrixGolgi matrix
"Tomographic Evidence for Continuous Turnover of Golgi Cisternae in Pichia pastoris". Molecular Biology of the Cell. 14 (6): ... "Tomographic evidence for continuous turnover of Golgi cisternae in Pichia pastoris". Molecular Biology of the Cell. 14 (6): ...
https://en.wikipedia.org/wiki/Golgi_matrix
Bile salt-dependent lipaseBile salt-dependent lipase
Murasugi A, Asami Y, Mera-Kikuchi Y (2001). "Production of recombinant human bile-salt-stimulated lipase in Pichia pastoris". ...
https://en.wikipedia.org/wiki/Bile_salt-dependent_lipase
Collagen, type XVIII, alpha 1Collagen, type XVIII, alpha 1
Feng Y, Cui LB, Liu CX, Ma QJ (2001). "[Inhibition effect in vitro of purified endostatin expressed in Pichia pastoris]". Sheng ...
https://en.wikipedia.org/wiki/Collagen,_type_XVIII,_alpha_1
Herman PhaffHerman Phaff
Pichia cactophila (1978) from decaying cacti and Drosophila flies feeding on them. Pichia heedii (1978) from the soft rot of ... Pichia opuntiae (1979) from the cladodes of Opuntia inermis in Australia and from the decaying parts of Cereoid cacti in North ... Starmer, W. T.; Phaff, H. J.; Miranda, M.; Miller, M. W. (1978). "Pichia cactophila, a New Species of Yeast Found in Decaying ... Phaff, H. J.; Starmer, W. T.; Miranda, M.; Miller, M. W. (1978). "Pichia heedii, a New Species of Yeast Indigenous to Necrotic ...
https://en.wikipedia.org/wiki/Herman_Phaff
GBA3GBA3
2002). "Functional expression of human liver cytosolic beta-glucosidase in Pichia pastoris. Insights into its role in the ...
https://en.wikipedia.org/wiki/GBA3
Scheffersomyces stipitisScheffersomyces stipitis
... (formerly Pichia stipitis) is a species of yeast, belonging to the "CUG Clade" of ascomycetous yeasts ... March 2007). "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis". Nature ...
https://en.wikipedia.org/wiki/Scheffersomyces_stipitis
S-Adenosylmethionine synthetase enzymeS-Adenosylmethionine synthetase enzyme
... synthetase from Pichia ciferrii". Bioprocess Biosyst Eng. 35 (1-2): 173-81. doi:10.1007/s00449-011-0640-x. PMID 21989639. S2CID ...
https://en.wikipedia.org/wiki/S-Adenosylmethionine_synthetase_enzyme
Subunit vaccineSubunit vaccine
"Recent advances in the production of recombinant subunit vaccines in Pichia pastoris". Bioengineered. 7 (3): 155-165. doi: ...
https://en.wikipedia.org/wiki/Subunit_vaccine
BabjeviellaBabjeviella
It was first described as Pichia inositovora in 1981 by Golubev, Blagod., Suetin & RS Trots. It was revised as Babjeviella ...
https://en.wikipedia.org/wiki/Babjeviella
Recombinant subunit vaccineRecombinant subunit vaccine
Wang M, Jiang S, Wang Y (April 2016). "Recent advances in the production of recombinant subunit vaccines in Pichia pastoris". ...
https://en.wikipedia.org/wiki/Recombinant_subunit_vaccine
Tools for Developing Successful Biologics - XS® Pichia Expression SystemTools for Developing Successful Biologics - XS® Pichia Expression System
The XS® Pichia Expression System allows you to develop scalable, robust and reliable processes to express your biologics drugs ... Tools for Developing Successful Biologics - XS® Pichia Expression System. 31 Dec 2020 ...
https://www.lonza.com/knowledge-center/biologics/brief/xs-ecoli-expression-system
Development and application of enzymatic substrate feeding strategies for small-scale microbial cultivations. Applied for...Development and application of enzymatic substrate feeding strategies for small-scale microbial cultivations. Applied for...
Applied for Escherichia coli, Pichia pastoris, and Lactobacillus salivarius cultivations Thesis event information Date and time ...
https://www.oulu.fi/en/theses/development-and-application-enzymatic-substrate-feeding-strategies-small-scale-microbial
AID 1083499 - Antimicrobial activity against Pichia kudriavzevii by macro dilution test - PubChemAID 1083499 - Antimicrobial activity against Pichia kudriavzevii by macro dilution test - PubChem
Antimicrobial activity against Pichia kudriavzevii by macro dilution test. ...
https://pubchem.ncbi.nlm.nih.gov/bioassay/1083499
Central carbon metabolism of the biocontrol yeast Pichia anomala - Open access publications in the SLU publication databaseCentral carbon metabolism of the biocontrol yeast Pichia anomala - Open access publications in the SLU publication database
The biocontrol yeast Pichia anomala prevents mould damage of moist cereal grain during malfunctioning airtight storage but it ... Influence of ethyl acetate production and ploidy on the anti-mould activity of Pichia anomala. FEMS Microbiol Lett 238:133-137 ... Metabolite profiles of the biocontrol yeast Pichia anomala J121 grown under oxygen limitation. Appl Microb Biotech 64:403-409. ... pichia, yeasts, metabolism, acetates, alcohol dehydrogenase, pyruvate decarboxylase, aldehydes, penicillium roquefortii. ...
https://pub.epsilon.slu.se/674/
Adaptive control of glycerol & methanol feeding in recombinant Pichia pastoris cultures: Impact on antibody titreAdaptive control of glycerol & methanol feeding in recombinant Pichia pastoris cultures: Impact on antibody titre
Pichia pastoris processes for heterolougous protein expression are typically run in three phases: a batch phase, an exponential ... Pichia pastoris processes for heterolougous protein expression are typically run in three phases: a batch phase, an exponential ... Adaptive control of glycerol & methanol feeding in recombinant Pichia pastoris cultures: Impact on antibody titre. Poster ...
https://www.technologynetworks.com/drug-discovery/posters/adaptive-control-of-glycerol-methanol-feeding-in-recombinant-pichia-pastoris-cultures-impact-on-antibody-titre-230333
for ELISA - Antigene from Pichia pastoris - Antibodies | BioVendor Research and Diagnostics Productsfor ELISA - Antigene from Pichia pastoris - Antibodies | BioVendor Research and Diagnostics Products
Pichia pastoris source of antigen 293 cell line (Human embryonic kidney) E. coli Freshly ejaculated human sperms HEK293 Human ... for ELISA - Antigene from Pichia pastoris - Antibodies. Product filter molecule Acylation-Stimulating Protein Adipocyte Fatty ...
https://www.biovendor.com/antibodies?application=ELISA&sourceOfAntigen=Pichia+pastoris
Purification and characterization of the first recombinant bird pancreatic lipase expressed in Pichia pastoris: The turkey |...Purification and characterization of the first recombinant bird pancreatic lipase expressed in Pichia pastoris: The turkey |...
The aim of the present study was to express TPL in the methylotrophic yeast Pichia pastoris in order to get a large amount of ... Using Q-PCR, the number of expression cassette integrated on Pichia genomic DNA was estimated to 5. The purified rTPL, with ... Pichia pastoris liquid cell cultures were grown in YPD medium containing 10 g yeast extract, 20 Bacto-peptone and 20 g D- ... The aim of the present study was to express TPL in the methylotrophic yeast Pichia pastoris in order to get a large amount of ...
https://lipidworld.biomedcentral.com/articles/10.1186/1476-511X-10-24
Molecular evidence on the occurrence of co-infection with Pichia guilliermondii and Wuchereria bancrofti in two filarial...Molecular evidence on the occurrence of co-infection with Pichia guilliermondii and Wuchereria bancrofti in two filarial...
The isolate was identified as Pichia guilliermondii and this fungus was found to exist in co-infection with Wuchereria ... Martini AV, Kurtzman CP, Meyer SA, ONeill EB: Two new species in the Pichia guilliermondii clade: Pichia caribbica sp. nov., ... Mukherjee, S., Mukherjee, N., Saini, P. et al. Molecular evidence on the occurrence of co-infection with Pichia guilliermondii ... The isolate was identified as Pichia guilliermondii and this fungus was found to exist in co-infection with Wuchereria ...
https://idpjournal.biomedcentral.com/articles/10.1186/2049-9957-3-13
Pichia - Log In
TBRC | Pichia sp. (TBRC 14422)
According to a precautionary measure taken by our organization over COVID-19 outbreak, we may not operate some services on a regular schedule. We apologize for the inconvenience that may have caused. Your understanding of the situation is greatly appreciated ...
https://tbrcnetwork.org/microb_detail.php?code=TBRC_14422
N-glycosylation site occupancy in human prostaglandin H synthases expressed in Pichia pastoris | SpringerPlus | Full TextN-glycosylation site occupancy in human prostaglandin H synthases expressed in Pichia pastoris | SpringerPlus | Full Text
In this study, human PGHS-1 and -2 (hPGHS-1 and -2) were expressed in the yeast Pichia pastoris. Recombinant hPGHS-2 was ... From: N-glycosylation site occupancy in human prostaglandin H synthases expressed in Pichia pastoris ...
https://springerplus.springeropen.com/articles/10.1186/2193-1801-3-436/tables/2
IJMS | Free Full-Text | The Heavy Chain 4F2hc Modulates the Substrate Affinity and Specificity of the Light Chains LAT1 and LAT2IJMS | Free Full-Text | The Heavy Chain 4F2hc Modulates the Substrate Affinity and Specificity of the Light Chains LAT1 and LAT2
For this purpose, the HATs and the light chains were expressed in the methylotrophic yeast Pichia pastoris and a radiolabel ... Byrne, B. Pichia pastoris as an expression host for membrane protein structural biology. Curr. Opin. Struct. Biol. 2015, 32C, 9 ... For Western blots, ~15 mg Pichia cells expressing HATs or LATs were suspended in 1 mL of 50 mM potassium phosphate pH 7.4, 1 mM ... The human HATs: 4F2hc-LAT1 and 4F2hc-LAT2, and LATs: LAT1 and LAT2 were expressed in the methylotrophic yeast Pichia pastoris. ...
https://www.mdpi.com/1422-0067/21/20/7573
Pichia Pastoris Source Kex2 Recombinant Protease With Animal Origin Free
Producing high quality Pichia Pastoris Source Kex2 Recombinant Protease With Animal Origin Free products. ... High quality Pichia Pastoris Source Kex2 Recombinant Protease With Animal Origin Free from China, Chinas leading Kex2 Protease ... Pichia Pastoris Source Kex2 Recombinant Protease With Animal Origin Free. Pichia Pastoris Source Kex2 Recombinant Protease With ... Pichia Pastoris. Appearance:. White Or Off-white Powder. Specific Activity:. ≥10.0 Units / Mg Pro.. Purity (SDS-PAGE):. Single ...
https://www.recombinanttrypsin.com/sale-8722990-pichia-pastoris-source-kex2-recombinant-protease-with-animal-origin-free.html
TRANSCRIPTPROM - Integrative and comparative analyses of response of Pichia pastorisTRANSCRIPTPROM - Integrative and comparative analyses of response of Pichia pastoris
Projects: Uncurated Protocols Library, TRANSCRIPTPROM - Integrative and comparative analyses of response of Pichia pastoris, ... Projects: Uncurated Protocols Library, TRANSCRIPTPROM - Integrative and comparative analyses of response of Pichia pastoris, ... Projects: TRANSCRIPTPROM - Integrative and comparative analyses of response of Pichia pastoris, FLOX: FLavin-containing ... FLOX: FLavin-containing OXidoreductases, PlanOvac, Muconic Acid Production, HIGHFLUX, Promoters 4 Pichia, An inventory of the ...
https://hub.ibisba.eu/projects/4
Scientific Overview of Pichia Pastoris Expression System - Curious Mind MagazineScientific Overview of Pichia Pastoris Expression System - Curious Mind Magazine
Scientific Overview of Pichia Pastoris Expression System. Proteins are ideal for various industries, including ... Understanding The Pichia Pastoris Expression System. The Pichia pastoris expression system has become one of the most widely ... Initial Application of Pichia Pastoris Expression System. In the early 1970s, the methylotrophic yeast pichia pastoris was used ... Reasons For Attractiveness of Pichia Pastoris. Today, the P. pastoris expression system is very appealing for recombinant ...
https://curiousmindmagazine.com/scientific-overview-of-pichia-pastoris-expression-system/
Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase...Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase...
Production of ApV1 in Pichia pastoris (Komagataella phaffi) was much lower than the wild-type enzyme due to the presence of the ... bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia ...
https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-021-01936-8/figures/2
Genomic diversity and meiotic recombination among isolates of the biotech yeast Komagataella phaffii (Pichia pastoris) |...Genomic diversity and meiotic recombination among isolates of the biotech yeast Komagataella phaffii (Pichia pastoris) |...
... and is one of the two species that were previously called Pichia pastoris. However, almost all laboratory work on K. phaffii ... From: Genomic diversity and meiotic recombination among isolates of the biotech yeast Komagataella phaffii (Pichia pastoris) ...
https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-019-1260-4/figures/1
Fast assimilation of mixed microbial (pichia nakasei, hansenula anomala) bod sensor by using various biological samples |...
Fast assimilation of mixed microbial (pichia nakasei, hansenula anomala) bod sensor by using various biological samples. Author ... The present study reveals that the yeast species Pichia nakasei, Hansenula anomala acted as a new alternate for BOD estimation ...
http://journalcra.com/article/fast-assimilation-mixed-microbial-pichia-nakasei-hansenula-anomala-bod-sensor-using-various
Characterization of the N-linked oligosaccharides from human chorionic gonadotropin expressed in the methylotrophic yeast...Characterization of the N-linked oligosaccharides from human chorionic gonadotropin expressed in the methylotrophic yeast...
Biologically active hCG has been successfully expressed in the yeast Pichia pastoris (phCG). In the context of structural ... Glycosylation; Pichia pastoris; Human Chorionic Gonadotropin; High-mannose-type N-glycans; Phosphorylation. ... of the N-linked oligosaccharides from human chorionic gonadotropin expressed in the methylotrophic yeast Pichia pastoris ... of the N-linked oligosaccharides from human chorionic gonadotropin expressed in the methylotrophic yeast Pichia pastoris ...
http://repository.ias.ac.in/60509/
Anamorph and Teleomorph Names for Candida Species | Fungal Diseases | CDCAnamorph and Teleomorph Names for Candida Species | Fungal Diseases | CDC
Pichia kudriavzevii. Candida krusei. Pichia caribbica/Meyerozyma caribbica. Candida fermentati. Kodamaea ohmeri. Candida ...
https://www.cdc.gov/fungal/lab-professionals/anamorph-and-teleomorph-names.html
5.1.3.5: UDP-arabinose 4-epimerase - BRENDA Enzyme Database5.1.3.5: UDP-arabinose 4-epimerase - BRENDA Enzyme Database
BRENDA - The Comprehensive Enzyme Information System
https://brenda-enzymes.org/all_enzymes.php?ecno=5.1.3.5&table=Pathway
Influence of CaCl2 on Penicillium digitatum, grapefruit peel tissue, and biocontrol activity of Pichia guilliermondii
Pilot Testing of Pichia guilliermondii: A Biocontrol Agent of Postharvest Diseases of Citrus Fruit ... Interactions between CaCl2, grapefruit peel tissue, Penicillium digitatum, and the yeast antagonist Pichia guilliermondii ... Interactions between CaCl2, grapefruit peel tissue, Penicillium digitatum, and the yeast antagonist Pichia guilliermondii ... of Pichia guilliermondii containing either 68 or 136 mM CaCl2 reduced the incidence of green mold from 27 to 3%. In large scale ...
https://volcaniarchive.agri.gov.il/skn/en/c6/e30636/Scientific_Publication/Influence_of_CaCl2_on_Penicillium_digitatum_grapef
Cloning of Lipase Gene From Thermomyces langinosus into Pichia pastoris with its Original Signal Peptide | Microbiology...Cloning of Lipase Gene From Thermomyces langinosus into Pichia pastoris with its Original Signal Peptide | Microbiology...
Cloning of Lipase Gene From Thermomyces langinosus into Pichia pastoris with its Original Signal Peptide Authors. * MILANI ... The aim of this research was to construct the Thermomyces lanuginosus lipase (TLL) gene into Pichia pastoris vector expression ... The correct plasmid was linearized and then was transformed in Pichia pastoris X-33 by electroporation method. Thermomyces ... Cloning of Lipase Gene From Thermomyces langinosus into Pichia pastoris with its Original Signal Peptide. Microbiology ...
https://jurnal.permi.or.id/index.php/mionline/article/view/573?articlesBySameAuthorPage=3
Expression, purification, and characterization of recombinant humanpar by YAĞMUR ÜNVER, ESABİ BAŞARAN KURBANOĞLU et al."Expression, purification, and characterization of recombinant humanpar" by YAĞMUR ÜNVER, ESABİ BAŞARAN KURBANOĞLU et al.
The main purpose of the present study was to perform the expression in Pichia pastoris X-33 of the human paraoxonase 1 (hPON1) ... The main purpose of the present study was to perform the expression in Pichia pastoris X-33 of the human paraoxonase 1 (hPON1) ... Expression, purification, and characterization of recombinant humanparaoxonase 1 (rhPON1) in Pichia pastoris ... in Pichia pastoris," Turkish Journal of Biology: Vol. 39: No. 4, Article 15. https://doi.org/10.3906/biy-1501-43 Available at: ...
https://journals.tubitak.gov.tr/biology/vol39/iss4/15/
MAPK/HOG signaling pathway induced stress-responsive damage repair a mechanism for Pichia pastoris to survive from hyperosmotic...
MAPK/HOG signaling pathway induced stress-responsive damage repair a mechanism for Pichia pastoris to survive from hyperosmotic ... MAPK/HOG signaling pathway induced stress-responsive damage repair a mechanism for Pichia pastoris to survive from hyperosmotic ... However, the weak ability of the biotechnical workhorse, Pichia pastoris, in surviving hyperosmotic stress suggests a unique ...
https://dspace.rpi.edu/handle/20.500.13015/5468
High level xylitol production by Pichia fermentans using non-detoxified xylose-rich sugarcane bagasse and olive pits...High level xylitol production by Pichia fermentans using non-detoxified xylose-rich sugarcane bagasse and olive pits...
The current study evaluated the potential of Pichia fermentans for manufacturing of xylitol from SCB and OP hydrolysates ... High level xylitol production by Pichia fermentans using non-detoxified xylose-rich sugarcane bagasse and olive pits ... Olea Saccharum Celulose Fermentação Hidrólise Pichia Xilitol Xilose Texto completo *https://www.ncbi.nlm.nih.gov/pmc/articles/ ...
https://pesquisa.bvsalud.org/odontologia/resource/pt/mdl-34592613
High-level expression of human arginase I in Pichia pastoris and its immobilization on chitosan to produce L-ornithine | BMC...High-level expression of human arginase I in Pichia pastoris and its immobilization on chitosan to produce L-ornithine | BMC...
The Pichia pastoris expression system offers the possibility of generating a large amount of recombinant protein. The ... The recombinant human arginase I (ARG I) was obtained using an optimized method with the Pichia pastoris GS115 as the host ... From: High-level expression of human arginase I in Pichia pastoris and its immobilization on chitosan to produce L-ornithine ...
https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12896-015-0184-2/tables/2
Integrating metabolic modeling and population heterogeneity analysis into optimizing recombinant protein production by...
Pichia) pastoris. Together they form a unique fingerprint. ... Pichia) pastoris. Chrispian W. Theron, Julio Berrios, Frank ...
https://pure.pucv.cl/en/publications/integrating-metabolic-modeling-and-population-heterogeneity-analy/fingerprints/
Genome sequences of Cyberlindnera fabianii 65, Pichia kudriavzevii 129, and Saccharomyces cerevisiae 131 isolated from...Genome sequences of Cyberlindnera fabianii 65, Pichia kudriavzevii 129, and Saccharomyces cerevisiae 131 isolated from...
Pichia kudriavzevii 129, and Saccharomyces cerevisiae 131 have been isolated from the microbiota of fermented masau fruits. C. ... Cyberlindnera fabianii 65, Pichia kudriavzevii 129, and Saccharomyces cerevisiae 131 have been isolated from the microbiota of ... Genome sequences of Cyberlindnera fabianii 65, Pichia kudriavzevii 129, and Saccharomyces cerevisiae 131 isolated from ...
https://www.pacb.com/publications/genome-sequences-of-cyberlindnera-fabianii-65-pichia-kudriavzevii-129-and-saccharomyces-cerevisiae-131-isolated-from-fermented-masau-fruits-in-zimbabwe/
Aquafeed.com | ADM leverages novel yeast feed additive to support better outcomes in aquacultureAquafeed.com | ADM leverages novel yeast feed additive to support better outcomes in aquaculture
AquaTrax is based on the novel yeast Pichia guilliermondii. This yeast has a unique morphology and structure that influences ...
https://aquafeed.com/products/new-products/adm-leverages-novel-yeast-feed-additive-to-support-better-outcomes-in-aquaculture/
Methylotrophic yeast pichia pastorisAnomalaHansenulaKomagataellaGuilliermondiiPastoris X-33KudriavzeviiRecombinant proteinSpeciesFermentansPeptideCandidaElectroporationProteinExpressionProductionAcidUniqueActiveExpressed in Pichia pastorisStipitisMethylotrophic yeastSaccharomycesStrainsHost Pichia pastorisExpressionCharacterizationProteinsEnzymeFermentationStrainBacillusCodon-optimizedBivalentGenomeSuccessfullySuitablePex19
Methylotrophic yeast pichia pastoris2
  • The aim of the present study was to express TPL in the methylotrophic yeast Pichia pastoris in order to get a large amount of this enzyme exhibiting interesting biochemical properties, to purify and characterize the recombinant enzyme. (biomedcentral.com)
  • In the early 1970s, the methylotrophic yeast pichia pastoris was used for the first time to produce single-cell protein. (curiousmindmagazine.com)
Anomala6
  • The biocontrol yeast Pichia anomala prevents mould damage of moist cereal grain during malfunctioning airtight storage but it can also spoil food and feed. (slu.se)
  • Physiological characteristics of the biocontrol yeast Pichia anomala J121. (slu.se)
  • Metabolite profiles of the biocontrol yeast Pichia anomala J121 grown under oxygen limitation. (slu.se)
  • Influence of ethyl acetate production and ploidy on the anti-mould activity of Pichia anomala. (slu.se)
  • Oxygen and glucose dependent regulation of central carbon metabolism in Pichia anomala. (slu.se)
  • The present study reveals that the yeast species Pichia nakasei, Hansenula anomala acted as a new alternate for BOD estimation. (journalcra.com)
Hansenula1
  • Pichia (Hansenula) was the major yeast species identified. (cdc.gov)
Komagataella1
  • Komagataella phaffii (Pichia pastoris) is likely one of the most extensively utilized yeast species in pharmaceutical and biotechnological industries, and, subsequently, additionally referred to as the biotech yeast. (merseybio.com)
Guilliermondii3
  • The isolate was identified as Pichia guilliermondii and this fungus was found to exist in co-infection with Wuchereria bancrofti in filarial patients. (biomedcentral.com)
  • Interactions between CaCl2, grapefruit peel tissue, Penicillium digitatum, and the yeast antagonist Pichia guilliermondii strain US-7 were investigated. (agri.gov.il)
  • AquaTrax is based on the novel yeast Pichia guilliermondii . (aquafeed.com)
Pastoris X-332
  • The correct plasmid was linearized and then was transformed in Pichia pastoris X-33 by electroporation method. (permi.or.id)
  • The main purpose of the present study was to perform the expression in Pichia pastoris X-33 of the human paraoxonase 1 (hPON1) enzyme, which is a mammalian serum protein. (tubitak.gov.tr)
Kudriavzevii1
  • Genome sequences of Cyberlindnera fabianii 65, Pichia kudriavzevii 129, and Saccharomyces cerevisiae 131 isolated from fermented masau fruits in Zimbabwe. (pacb.com)
Recombinant protein1
  • Cusabio has three technical service platform of protein purification, such as expression of recombinant protein in E. coli and Pichia pastoris or natural protein purification. (businesslistings.net.au)
Species1
  • Included C. holmii , C. valida , Candida species, and Pichia species. (cdc.gov)
Fermentans2
  • High level xylitol production by Pichia fermentans using non-detoxified xylose-rich sugarcane bagasse and olive pits hydrolysates. (bvsalud.org)
  • The current study evaluated the potential of Pichia fermentans for manufacturing of xylitol from SCB and OP hydrolysates through co- fermentation strategy. (bvsalud.org)
Peptide2
  • The aim of this research was to construct the Thermomyces lanuginosus lipase (TLL) gene into Pichia pastoris vector expression with TLL original signal peptide. (permi.or.id)
  • Nourishing Donkey Milk eye cream which provides complete protection against wrinkles, swelling and dark circles, while combating oxidative stress from environmental pollutants, thanks to the innovative Yeast Pichia heedii peptide complex it contains. (grecoroots.com)
Candida2
  • October 23, 2011 (Boston, Massachusetts) - By characterizing, for the first time, the core oral mycobiome and bacteriome of HIV-infected patients and control individuals, researchers at The Cleveland Clinic in Ohio have shown that Pichia , a genus in the yeast family, inhibits Candida from forming biofilms, suggesting that changes in oral microbiota significantly affect human health and disease. (medscape.com)
  • Importantly, the presence of Pichia correlated with an absence of Candida , and vice versa, Dr. Ghannoum said. (medscape.com)
Electroporation1
  • High efficiency transformation by electroporation of Pichia pastoris pretreated with lithium acetate and dithiothreitol. (cocites.com)
Protein1
  • Pichia pastoris processes for heterolougous protein expression are typically run in three phases: a batch phase, an exponential feeding fed-batch phase and a long oxygen transfer limitation (OTL) phase. (technologynetworks.com)
Expression5
  • The XS® Pichia Expression System allows you to develop scalable, robust and reliable processes to express your biologics drugs with improved productivity, ultimately accelerating research to bring new therapies to patients in need of treatment. (lonza.com)
  • Using Q-PCR, the number of expression cassette integrated on Pichia genomic DNA was estimated to 5. (biomedcentral.com)
  • Thanks to IBISBA prompt response after the submission of the application, and the network of IBISBA facilities, Prof. Calik was able to rapidly and successfully conduct a series of experiments for integrative and comparative analyses of response of Pichia pastoris reaction network to expression systems designed with engineered promoters. (ibisba.eu)
  • The Pichia pastoris expression system has become one of the most widely utilized. (curiousmindmagazine.com)
  • Expression of porcine epidermal growth factor in Pichia pastoris and its biology activity in early-weaned piglets. (sinica.edu.tw)
Production1
  • The toxicity-reduced hemicellulosic hydrolysates that were obtained through a two-step treatment at optimized conditions were fermented using Pichia stipitis for ethanol production, without any further detoxification. (preprints.org)
Acid2
  • Ultra-concentrated Hyaluronic Acid ampoules with Pichia Mushroom Prebiotic Ferment and effectively firming Therapeutic Plants, including Ziziphus, Maral Root, Centella Asiatica, Goji, and Kigelia. (foxyskin.me)
  • Ultra-concentrated Hyaluronic Acid ampoules with Pichia Mushroom Prebiotic Ferment and Therapeutic Plants Dragon's Blood and Centella Asiatica combined with medicinal extracts from Bacopa, Moringa, and Lapsana, for antioxidant, oxygenating, protective. (foxyskin.me)
Unique1
  • However, the weak ability of the biotechnical workhorse, Pichia pastoris, in surviving hyperosmotic stress suggests a unique regulatory mechanism needing further investigation. (rpi.edu)
Active1
  • Biologically active hCG has been successfully expressed in the yeast Pichia pastoris (phCG). (ias.ac.in)
Expressed in Pichia pastoris4
  • the gene was heterologously expressed in Pichia pastoris KM71H. (ruc.dk)
  • Recombinant Kex2 protease is a genetically engineered protein expressed in Pichia pastoris and with equivalent properties compared to native Saccharomyces cerevisiae Kex2. (recombinanttrypsin.com)
  • In this study, the biologically active and soluble fragment (residues 33-182) of human (CSF-1) was cloned from K562 cell line and expressed in Pichia pastoris. (edu.au)
  • The novel cellobiohydrolase gene ctcel7 was identified from Chaetomium thermophilum , and its recombinant protein CtCel7, a member of glycoside hydrolase family 7, was heterologously expressed in Pichia pastoris and biochemically characterized. (frontiersin.org)
Stipitis7
  • Through the process of fermentation using yeast Pichia stipitis, glucose is converted into ethanol and carbon dioxide. (unri.ac.id)
  • Preparation of starter do with the yeast Pichia stipitis inoculum in the fermentation medium so that the yeast is able to adapt and ready for fermentation. (unri.ac.id)
  • Cell immobilization technique was applied in this study in order to examine effect of immobilized Pichia stipitis TISTR5806 on bioethanol production. (kmutnb.ac.th)
  • In this study, continuous SHcF (separate hydrolysis and co-fermentation) and SScF (simultaneous saccharification and co-fermentation) were evaluated for ethanol production from alkaline pretreated sugarcane bagasse using Zymomonas mobilis (PVA immobilized cells) and Pichia stipitis (suspended cells). (edu.hk)
  • 1] "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis. (tcdb.org)
  • The yeast Pichia stipitis was, however, found less robust in these hydrolyzates. (dissertations.se)
  • At the time, Jeffries was working with Scheffersomyces (Pichia) stipitis and Spathaspora passalidarum , specialized yeast strains found in the stomachs of beetles. (wpi.edu)
Methylotrophic yeast8
  • The methylotrophic yeast Pichia pastoris is frequently used for the production of recombinant proteins. (biomedcentral.com)
  • The methylotrophic yeast Pichia pastoris (syn. (biomedcentral.com)
  • The aim of the present study was to express TPL in the methylotrophic yeast Pichia pastoris in order to get a large amount of this enzyme exhibiting interesting biochemical properties, to purify and characterize the recombinant enzyme. (biomedcentral.com)
  • The methylotrophic yeast Pichia pastoris is one of the most important eukaryotic systems for industrial protein production. (springeropen.com)
  • Product description： Pichia pastoris is a species of methylotrophic yeast. (food-bacteria.net)
  • Valeraldehyde can be produced for marketing as a natural flavoring compound in an enzymatic bioindustrial process from pentanol using the methylotrophic yeast, Pichia pastoris (Williams et al . (nih.gov)
  • This database hosts up-to-date annotation information of the genome of the methylotrophic yeast Pichia pastoris ( Komagataella spp. (boku.ac.at)
  • nov. and the transfer of Pichia pseudopastoris to the methylotrophic yeast genus Komagataella. (boku.ac.at)
Saccharomyces4
  • Pichia , Kloeckera , Metschnikowia and a very small number of Saccharomyces cerevisiae (Fleet et al. (ithaka-journal.net)
  • Current microbial expression options include Escherichia coli, yeasts ( Saccharomyces cerevisiae, Pichia pastoris ) and Pseudomonas fluorescens , on which the Pf &ebar;nex system is based ( 5 , 6 , 7 , 8 ). (bioprocessintl.com)
  • Today, such muteins can be routinely expressed in Escherichia coli , Saccharomyces cerevisiae , and Pichia pastoris , but the functional requirement for modifications such as disulfide bridges or delicate N - and O -glycosylation present at, e.g., the linker domains of the cellulase proteins render most of these expression hosts inappropriate. (springer.com)
  • Yeasts species such as Saccharomyces cerevisiae and Pichia pastoris have been successfully used as host organisms to produce subunit vaccine antigens. (fems-microbiology.org)
Strains7
  • Optimizing lab assays of Bacillus and/or Pichia strains for protein productivity and predictive power across scales. (biospace.com)
  • Hands-on experience with optimizing growth conditions and target protein expression in Bacillus and/or Pichia strains. (biospace.com)
  • Further strains were isolated from trees in California by H. Phaff, who named the species Pichia pastoris in 1956 [2]. (boku.ac.at)
  • The established Pichia pastoris protein production platforms are based on strains of either K. phaffii or K. pastoris [7]. (boku.ac.at)
  • To avoid confusion and to include all strains employed in biotechnology we use the established name Pichia pastoris here as a synonym for all Komagataella species, giving reference to the different species for different strains, as listed in the table below. (boku.ac.at)
  • 2009. Biotechnological strains of Komagataella (Pichia) pastoris are Komagataella phaffii as determined from multigene sequence analysis. (boku.ac.at)
  • The maximum production of biofilm was detected in the Pichia strains. (bvsalud.org)
Host Pichia pastoris3
  • A gene encoding cellobiose dehydrogenase (CDH) from Neurospora crassa strain FGSC 2489 has been cloned and expressed in the heterologous host Pichia pastoris, under the control of the AOX1 methanol inducible promoter. (usda.gov)
  • 2009. Genome sequence of the recombinant protein production host Pichia pastoris. (boku.ac.at)
  • 2009. Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris. (boku.ac.at)
Expression12
  • The Pichia Classic Expression System has been licensed to more than 200 companies in the biotechnology, pharmaceutical, animal health and food industries. (rctech.com)
  • For more information on the Pichia Classic Expression System and RCT's licensing program for the technology, please visit www.pichia.com . (rctech.com)
  • In the present study, Bm-TFF2 gene was amplified by polymerase chain reaction (PCR) from its cDNA and cloned into Pichia pastoris expression vector pPIC9K containing AOX1 promoter and α-factor leader sequence. (zoores.ac.cn)
  • Sign up to our mailing list to keep up with latest developments and information around Pichia based protein expression. (validogen.com)
  • We report here expression of biologically active hCG and its subunits using a yeast expression system, Pichia pastoris. (iisc.ac.in)
  • Thus the Pichia expression system is capable of hyperexpressing properly folded, biologically active hGG and is suitable for structure-function studies of the hormone. (iisc.ac.in)
  • Expression of Hybrid Peptide EF-1 in Pichia pastoris, Its Purification, and Antimicrobial Characterization. (illumina.com)
  • The effect of dissolved oxygen (DO) tension on heterologous protein expression by the Pichia system was investigated by applying DO control bands of different set points and bandwidths in the DO-stat fed-batch fermentation. (hanyang.ac.kr)
  • Using Q-PCR, the number of expression cassette integrated on Pichia genomic DNA was estimated to 5. (biomedcentral.com)
  • Pichia is widely used for protein expression using recombinant DNA techniques. (food-bacteria.net)
  • High-level expression of human CH2 domain from the Fc region in Pichia pastoris and preparation of anti-CH2 antibodies. (sojo-u.ac.jp)
  • Here, we compare the soluble recombinant NTS-DBL1alpha(1)-varO domain (NTS: N-terminal segment) obtained from E. coli, Pichia pastoris and baculovirus/insect cell expression systems. (pasteur.fr)
Characterization2
  • Characterization of Human Recombinant N-Acetylgalactosamine-6-Sulfate Sulfatase Produced in Pichia pastoris as Potential Enzyme for Mucopolysaccharidosis IVA Treatment. (nih.gov)
  • Characterization of deamidation at Asn138 in L-chain of recombinant humanized Fab expressed from Pichia pastoris. (sojo-u.ac.jp)
Proteins1
  • This bioreactor line is for the cultivation of Pichia pastoris to manufacture enzymes and proteins. (bioreactors.net)
Enzyme3
  • A mixed feeding strategy (co-feeding of complex carbon sources with methanol) has become a common practice for process development in Pichia pastoris to increase cell biomass and enzyme production levels. (springeropen.com)
  • Designing and performing high-throughput enzyme assays and analytical methods to evaluate enzyme function, within the context of protein libraries expressed in Pichia or Bacillus . (biospace.com)
  • We then expressed the gene in a yeast Pichia pastoris, purified the enzyme, and characterized several enzymatic properties. (usda.gov)
Fermentation1
  • Microbiosci announced the release of its Pichia Pastoris Fermentation to support the development of microbiological research. (assamreporter.in)
Strain5
  • VALIDOGEN has recently broadened its strain development capabilities with a microbioreactor system serving as a time- and resource-saving tool for Pichia pastoris strain development. (validogen.com)
  • In addition to the benefits for VALIDOGEN`s Pichia strain development procedures, microbioreactors represent an appealing alternative to larger lab-scale bioreactors for cultivation process development including Design of Experiment (DoE) programs. (validogen.com)
  • In order to demonstrate the usefulness of microbioreactors in bioreactor cultivation process development, we performed a DoE based study with a Pichia pastoris strain producing a bivalent VHH. (validogen.com)
  • In addition, Impossible Foods' SLH is derived from a strain of GM Pichia pastoris yeast. (worldhealth.net)
  • Royle KE & Polizzi K A streamlined cloning workflow minimising the time-to-strain pipeline for Pichia pastoris. (abcam.com)
Bacillus1
  • Developing and implementing protocols for Bacillus and/or Pichia in shake flasks and microtiter plates. (biospace.com)
Codon-optimized2
  • Codon optimized keratinase gene successfully cloned in Pichia pastoris. (niist.res.in)
  • The chimeric recombinant protein (AMA166-MSP119) was recently expressed in yeast Pichia pastoris using synthetic codon optimized genes. (fapesp.br)
Bivalent1
  • A bivalent subunit vaccine efficiently produced in Pichia pastoris against SARS-CoV-2 and emerging variants. (cdc.gov)
Genome2
  • 2011. High-quality genome sequence of Pichia pastoris CBS7435. (boku.ac.at)
  • Vector for integration into the Pichia genome. (biogrammatics.com)
Successfully1
  • SDS-PAGE gel and western blotting analysis revealed that the recombinant lectin was expressed successfully in Pichia yeast. (nih.gov)
Suitable1
  • The hands-on-pichia course is suitable for practitioners and researchers who want to expand their level of expertise as well as pr. (tugraz.at)
Pex191
  • To this end, using deletion mutants, we mapped the Pex19 regions required for peroxisome biogenesis in the yeast Pichia pastoris. (divbiolchem.org)
IJMS | Free Full-Text | The Heavy Chain 4F2hc Modulates the Substrate Affinity and Specificity of the Light Chains LAT1 and LAT2
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Recently Published GRAS Notices and FDA Letters | FDA
Recently Published GRAS Notices and FDA Letters | FDA (fda.gov)
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Genomic insights into the phylogeny and biomass-degrading enzymes of rumen ciliates | The ISME Journal
Genomic insights into the phylogeny and biomass-degrading enzymes of rumen ciliates | The ISME Journal (nature.com)
Structural mechanism of plant aquaporin gating | Nature
Structural mechanism of plant aquaporin gating | Nature (nature.com)
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Deacetylation Followed by Fractionation of Yellow Poplar Sawdust for the Production of Toxicity-Reduced Hemicellulosic Sugar...
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Frontiers | Positive Charges on the Surface of Thaumatin Are Crucial for the Multi-Point Interaction with the Sweet Receptor
Frontiers | Positive Charges on the Surface of Thaumatin Are Crucial for the Multi-Point Interaction with the Sweet Receptor (frontiersin.org)
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One Fungus May Keep Another in Check for a Healthy Mouth (medscape.com)
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Current Events - TU Graz (tugraz.at)
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SGN Unigene - Show All Stored BLAST Hits - Sol Genomics Network (sgn.cornell.edu)
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Overview: Human & Biotechnology - TU Graz (tugraz.at)
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Les 65 vols de la saison 2020-2021 du dimanche 08/08/2021 | Parapente (parapente.ffvl.fr)
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Evolved strains of Scheffersomyces stipitis achieving high ethanol productivity on acid- and base-pretreated biomass... (biotechnologyforbiofuels.biomedcentral.com)
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High-temperature ethanol fermentation from pineapple waste hydrolysate and gene expression analysis of thermotolerant yeast... (nature.com)
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