A phosphoprotein that was initially identified as a major target of DOPAMINE activated ADENYLYL CYCLASE in the CORPUS STRIATUM. It regulates the activities of PROTEIN PHOSPHATASE-1 and PROTEIN KINASE A, and it is a key mediator of the biochemical, electrophysiological, transcriptional, and behavioral effects of DOPAMINE.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
A group of enzymes removing the SERINE- or THREONINE-bound phosphate groups from a wide range of phosphoproteins, including a number of enzymes which have been phosphorylated under the action of a kinase. (Enzyme Nomenclature, 1992)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The sum of the weight of all the atoms in a molecule.
A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein.
Monomeric subunits of primarily globular ACTIN and found in the cytoplasmic matrix of almost all cells. They are often associated with microtubules and may play a role in cytoskeletal function and/or mediate movement of the cell or the organelles within the cell.
A family of synaptic vesicle-associated proteins involved in the short-term regulation of NEUROTRANSMITTER release. Synapsin I, the predominant member of this family, links SYNAPTIC VESICLES to ACTIN FILAMENTS in the presynaptic nerve terminal. These interactions are modulated by the reversible PHOSPHORYLATION of synapsin I through various signal transduction pathways. The protein is also a substrate for cAMP- and CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASES. It is believed that these functional properties are also shared by synapsin II.
A ubiquitous phosphoprotein that serves as an intracellular substrate for a variety of SIGNAL TRANSDUCTION PATHWAYS. PHOSPHORYLATION of stathmin occurs during CELL CYCLE progression, and stathmin functions as a microtubule-destabilizing protein that promotes MICROTUBULE depolymerization during INTERPHASE and late MITOSIS. Stathmin is expressed at very high levels in a variety of human CANCERS.
Proteins found in any species of virus.
Unstable isotopes of phosphorus that decay or disintegrate emitting radiation. P atoms with atomic weights 28-34 except 31 are radioactive phosphorus isotopes.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
The phosphoric acid ester of serine.
The phosphoric acid ester of threonine. Used as an identifier in the analysis of peptides, proteins, and enzymes.
Established cell cultures that have the potential to propagate indefinitely.
An egg yolk phosphoglycoprotein which contains about 90% of the yolk protein phosphorus. It is synthesized in the liver of the hen and transferred to the developing oocyte, where it is bound to lipoproteins within the yolk granules.
Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels.
Analysis of PEPTIDES that are generated from the digestion or fragmentation of a protein or mixture of PROTEINS, by ELECTROPHORESIS; CHROMATOGRAPHY; or MASS SPECTROMETRY. The resulting peptide fingerprints are analyzed for a variety of purposes including the identification of the proteins in a sample, GENETIC POLYMORPHISMS, patterns of gene expression, and patterns diagnostic for diseases.
Proteins found in the microtubules.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis.
A negatively-charged extracellular matrix protein that plays a role in the regulation of BONE metabolism and a variety of other biological functions. Cell signaling by osteopontin may occur through a cell adhesion sequence that recognizes INTEGRIN ALPHA-V BETA-3.
Glycoproteins which contain sialic acid as one of their carbohydrates. They are often found on or in the cell or tissue membranes and participate in a variety of biological activities.
A non-essential amino acid occurring in natural form as the L-isomer. It is synthesized from GLYCINE or THREONINE. It is involved in the biosynthesis of PURINES; PYRIMIDINES; and other amino acids.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Proteins conjugated with nucleic acids.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
The rate dynamics in chemical or physical systems.
The type species of LYSSAVIRUS causing rabies in humans and other animals. Transmission is mostly by animal bites through saliva. The virus is neurotropic multiplying in neurons and myotubes of vertebrates.
Viral proteins that are components of the mature assembled VIRUS PARTICLES. They may include nucleocapsid core proteins (gag proteins), enzymes packaged within the virus particle (pol proteins), and membrane components (env proteins). These do not include the proteins encoded in the VIRAL GENOME that are produced in infected cells but which are not packaged in the mature virus particle,i.e. the so called non-structural proteins (VIRAL NONSTRUCTURAL PROTEINS).
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.
A ubiquitous casein kinase that is comprised of two distinct catalytic subunits and dimeric regulatory subunit. Casein kinase II has been shown to phosphorylate a large number of substrates, many of which are proteins involved in the regulation of gene expression.
A genus of the family RHABDOVIRIDAE that includes RABIES VIRUS and other rabies-like viruses.
A non-essential amino acid. In animals it is synthesized from PHENYLALANINE. It is also the precursor of EPINEPHRINE; THYROID HORMONES; and melanin.
A species in the genus Bornavirus, family BORNAVIRIDAE, causing a rare and usually fatal encephalitic disease in horses and other domestic animals and possibly deer. Its name derives from the city in Saxony where the condition was first described in 1894, but the disease occurs in Europe, N. Africa, and the Near East.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
Viral proteins found in either the NUCLEOCAPSID or the viral core (VIRAL CORE PROTEINS).
The parts of a macromolecule that directly participate in its specific combination with another molecule.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
A specific inhibitor of phosphoserine/threonine protein phosphatase 1 and 2a. It is also a potent tumor promoter. (Thromb Res 1992;67(4):345-54 & Cancer Res 1993;53(2):239-41)
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
An amino acid that occurs in endogenous proteins. Tyrosine phosphorylation and dephosphorylation plays a role in cellular signal transduction and possibly in cell growth control and carcinogenesis.
A zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
The type species of MORBILLIVIRUS and the cause of the highly infectious human disease MEASLES, which affects mostly children.
Inorganic salts of phosphoric acid.
The hard portion of the tooth surrounding the pulp, covered by enamel on the crown and cementum on the root, which is harder and denser than bone but softer than enamel, and is thus readily abraded when left unprotected. (From Jablonski, Dictionary of Dentistry, 1992)
Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
A protein-nucleic acid complex which forms part or all of a virion. It consists of a CAPSID plus enclosed nucleic acid. Depending on the virus, the nucleocapsid may correspond to a naked core or be surrounded by a membranous envelope.
A eukayrotic protein serine-threonine phosphatase subtype that dephosphorylates a wide variety of cellular proteins. The enzyme is comprised of a catalytic subunit and regulatory subunit. Several isoforms of the protein phosphatase catalytic subunit exist due to the presence of multiple genes and the alternative splicing of their mRNAs. A large number of proteins have been shown to act as regulatory subunits for this enzyme. Many of the regulatory subunits have additional cellular functions.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
A group of protein-serine-threonine kinases that was originally identified as being responsible for the PHOSPHORYLATION of CASEINS. They are ubiquitous enzymes that have a preference for acidic proteins. Casein kinases play a role in SIGNAL TRANSDUCTION by phosphorylating a variety of regulatory cytoplasmic and regulatory nuclear proteins.
Protein kinases that catalyze the PHOSPHORYLATION of TYROSINE residues in proteins with ATP or other nucleotides as phosphate donors.
An enzyme that deactivates glycogen phosphorylase a by releasing inorganic phosphate and phosphorylase b, the inactive form. EC
Proteins prepared by recombinant DNA technology.
An essential amino acid occurring naturally in the L-form, which is the active form. It is found in eggs, milk, gelatin, and other proteins.
A group of cyclic GMP-dependent enzymes that catalyze the phosphorylation of SERINE or THREONINE residues of proteins.
A group of enzymes that are dependent on CYCLIC AMP and catalyze the phosphorylation of SERINE or THREONINE residues on proteins. Included under this category are two cyclic-AMP-dependent protein kinase subtypes, each of which is defined by its subunit composition.
A broad category of carrier proteins that play a role in SIGNAL TRANSDUCTION. They generally contain several modular domains, each of which having its own binding activity, and act by forming complexes with other intracellular-signaling molecules. Signal-transducing adaptor proteins lack enzyme activity, however their activity can be modulated by other signal-transducing enzymes
The systematic study of the complete complement of proteins (PROTEOME) of organisms.
A nervous tissue specific protein which is highly expressed in NEURONS during development and NERVE REGENERATION. It has been implicated in neurite outgrowth, long-term potentiation, SIGNAL TRANSDUCTION, and NEUROTRANSMITTER release. (From Neurotoxicology 1994;15(1):41-7) It is also a substrate of PROTEIN KINASE C.
A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
A genus of the family RHABDOVIRIDAE that infects a wide range of vertebrates and invertebrates. The type species is VESICULAR STOMATITIS INDIANA VIRUS.
Transport proteins that carry specific substances in the blood or across cell membranes.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
The protein complement of an organism coded for by its genome.
Amino acids that contain phosphorus as an integral part of the molecule.
A group of hydrolases which catalyze the hydrolysis of monophosphoric esters with the production of one mole of orthophosphate. EC 3.1.3.
A phosphoprotein phosphatase subtype that is comprised of a catalytic subunit and two different regulatory subunits. At least two genes encode isoforms of the protein phosphatase catalytic subunit, while several isoforms of regulatory subunits exist due to the presence of multiple genes and the alternative splicing of their mRNAs. Protein phosphatase 2 acts on a broad variety of cellular proteins and may play a role as a regulator of intracellular signaling processes.
An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
The type species of VESICULOVIRUS causing a disease symptomatically similar to FOOT-AND-MOUTH DISEASE in cattle, horses, and pigs. It may be transmitted to other species including humans, where it causes influenza-like symptoms.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Major constituent of the cytoskeleton found in the cytoplasm of eukaryotic cells. They form a flexible framework for the cell, provide attachment points for organelles and formed bodies, and make communication between parts of the cell possible.
Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)
Proteins associated with the inner surface of the lipid bilayer of the viral envelope. These proteins have been implicated in control of viral transcription and may possibly serve as the "glue" that binds the nucleocapsid to the appropriate membrane site during viral budding from the host cell.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Proteins that regulate the signaling activity of GTP-BINDING PROTEINS. They are divided into three categories depending upon whether they stimulate GTPase activity (GTPASE-ACTIVATING PROTEINS), inhibit release of GDP; (GUANINE NUCLEOTIDE DISSOCIATION INHIBITORS); or exchange GTP for GDP; (GUANINE NUCLEOTIDE EXCHANGE FACTORS).
Within most types of eukaryotic CELL NUCLEUS, a distinct region, not delimited by a membrane, in which some species of rRNA (RNA, RIBOSOMAL) are synthesized and assembled into ribonucleoprotein subunits of ribosomes. In the nucleolus rRNA is transcribed from a nucleolar organizer, i.e., a group of tandemly repeated chromosomal genes which encode rRNA and which are transcribed by RNA polymerase I. (Singleton & Sainsbury, Dictionary of Microbiology & Molecular Biology, 2d ed)
A genus in the subfamily PARAMYXOVIRINAE, family PARAMYXOVIRIDAE. It contains species that infect and cause potentially fatal disease in a number of host species, including humans.
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A family of spherical viruses, of the order MONONEGAVIRALES, somewhat larger than the orthomyxoviruses, and containing single-stranded RNA. Subfamilies include PARAMYXOVIRINAE and PNEUMOVIRINAE.
Proteins and peptides that are involved in SIGNAL TRANSDUCTION within the cell. Included here are peptides and proteins that regulate the activity of TRANSCRIPTION FACTORS and cellular processes in response to signals from CELL SURFACE RECEPTORS. Intracellular signaling peptide and proteins may be part of an enzymatic signaling cascade or act through binding to and modifying the action of other signaling factors.
A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, infecting the salivary glands, liver, spleen, lungs, eyes, and other organs, in which they produce characteristically enlarged cells with intranuclear inclusions. Infection with Cytomegalovirus is also seen as an opportunistic infection in AIDS.
A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Compounds of the general formula R-O-R arranged in a ring or crown formation.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
A family of bullet-shaped viruses of the order MONONEGAVIRALES, infecting vertebrates, arthropods, protozoa, and plants. Genera include VESICULOVIRUS; LYSSAVIRUS; EPHEMEROVIRUS; NOVIRHABDOVIRUS; Cytorhabdovirus; and Nucleorhabdovirus.
Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
Proteins found in ribosomes. They are believed to have a catalytic function in reconstituting biologically active ribosomal subunits.
The functional hereditary units of VIRUSES.
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
A trace element with atomic symbol Mn, atomic number 25, and atomic weight 54.94. It is concentrated in cell mitochondria, mostly in the pituitary gland, liver, pancreas, kidney, and bone, influences the synthesis of mucopolysaccharides, stimulates hepatic synthesis of cholesterol and fatty acids, and is a cofactor in many enzymes, including arginase and alkaline phosphatase in the liver. (From AMA Drug Evaluations Annual 1992, p2035)
The pH in solutions of proteins and related compounds at which the dipolar ions are at a maximum.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
Proteins encoded by a VIRAL GENOME that are produced in the organisms they infect, but not packaged into the VIRUS PARTICLES. Some of these proteins may play roles within the infected cell during VIRUS REPLICATION or act in regulation of virus replication or VIRUS ASSEMBLY.
Guanosine cyclic 3',5'-(hydrogen phosphate). A guanine nucleotide containing one phosphate group which is esterified to the sugar moiety in both the 3'- and 5'-positions. It is a cellular regulatory agent and has been described as a second messenger. Its levels increase in response to a variety of hormones, including acetylcholine, insulin, and oxytocin and it has been found to activate specific protein kinases. (From Merck Index, 11th ed)
Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.
A metallic element that has the atomic symbol Mg, atomic number 12, and atomic weight 24.31. It is important for the activity of many enzymes, especially those involved in OXIDATIVE PHOSPHORYLATION.
Antibodies produced by a single clone of cells.
Ribonucleic acid that makes up the genetic material of viruses.
Predeciduous teeth present at birth. They may be well formed and normal or may represent hornified epithelial structures without roots. They are found on the gingivae over the crest of the ridge and arise from accessory buds of the dental lamina ahead of the deciduous buds or from buds of the accessory dental lamina. (From Jablonski, Dictionary of Dentistry, 1992)
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
A hereditary disorder occurring in two forms: the complete form (Franceschetti's syndrome) is characterized by antimongoloid slant of the palpebral fissures, coloboma of the lower lid, micrognathia and hypoplasia of the zygomatic arches, and microtia. It is transmitted as an autosomal trait. The incomplete form (Treacher Collins syndrome) is characterized by the same anomalies in less pronounced degree. It occurs sporadically, but an autosomal dominant mode of transmission is suspected. (Dorland, 27th ed)
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by radioactive material within the object. (Dorland, 27th ed)
An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Proteins found mainly in icosahedral DNA and RNA viruses. They consist of proteins directly associated with the nucleic acid inside the NUCLEOCAPSID.
A genus of ciliate protozoa that is often large enough to be seen by the naked eye. Paramecia are commonly used in genetic, cytological, and other research.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
A species of MORBILLIVIRUS causing cattle plague, a disease with high mortality. Sheep, goats, pigs, and other animals of the order Artiodactyla can also be infected.
Proteins which bind calmodulin. They are found in many tissues and have a variety of functions including F-actin cross-linking properties, inhibition of cyclic nucleotide phosphodiesterase and calcium and magnesium ATPases.
A CELL LINE derived from the kidney of the African green (vervet) monkey, (CERCOPITHECUS AETHIOPS) used primarily in virus replication studies and plaque assays.
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.
Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Five-membered heterocyclic ring structures containing an oxygen in the 1-position and a nitrogen in the 3-position, in distinction from ISOXAZOLES where they are at the 1,2 positions.
Techniques used to demonstrate or measure an immune response, and to identify or measure antigens using antibodies.
Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.
Elongated gray mass of the neostriatum located adjacent to the lateral ventricle of the brain.
A mixture of related phosphoproteins occurring in milk and cheese. The group is characterized as one of the most nutritive milk proteins, containing all of the common amino acids and rich in the essential ones.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.
Electrophoresis in which paper is used as the diffusion medium. This technique is confined almost entirely to separations of small molecules such as amino acids, peptides, and nucleotides, and relatively high voltages are nearly always used.
Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. A commonly observed structure may be composed of a CONSERVED SEQUENCE which can be represented by a CONSENSUS SEQUENCE.
A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC
A family of low molecular weight proteins that bind ACTIN and control actin polymerization. They are found in eukaryotes and are ubiquitously expressed.
Chromatographic techniques in which the mobile phase is a liquid.
The outer protein protective shell of a virus, which protects the viral nucleic acid.
A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The process whereby calcium salts are deposited in the dental enamel. The process is normal in the development of bones and teeth. (Boucher's Clinical Dental Terminology, 4th ed, p43)
Src-family kinases that associate with T-CELL ANTIGEN RECEPTOR and phosphorylate a wide variety of intracellular signaling molecules.
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
A plasma membrane exchange glycoprotein transporter that functions in intracellular pH regulation, cell volume regulation, and cellular response to many different hormones and mitogens.
An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus.
Complexes of RNA-binding proteins with ribonucleic acids (RNA).
A tyrosine-specific protein kinase encoded by the v-src oncogene of ROUS SARCOMA VIRUS. The transforming activity of pp60(v-src) depends on both the lack of a critical carboxy-terminal tyrosine phosphorylation site at position 527, and the attachment of pp60(v-src) to the plasma membrane which is accomplished by myristylation of its N-terminal glycine.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
A genus of the family PARAMYXOVIRIDAE (subfamily PARAMYXOVIRINAE) where the virions of most members have hemagglutinin but not neuraminidase activity. All members produce both cytoplasmic and intranuclear inclusion bodies. MEASLES VIRUS is the type species.
Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.
Regions of AMINO ACID SEQUENCE similarity in the SRC-FAMILY TYROSINE KINASES that fold into specific functional tertiary structures. The SH1 domain is a CATALYTIC DOMAIN. SH2 and SH3 domains are protein interaction domains. SH2 usually binds PHOSPHOTYROSINE-containing proteins and SH3 interacts with CYTOSKELETAL PROTEINS.
The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.
Any of the eight frontal teeth (four maxillary and four mandibular) having a sharp incisal edge for cutting food and a single root, which occurs in man both as a deciduous and a permanent tooth. (Jablonski, Dictionary of Dentistry, 1992, p820)
Techniques for removal by adsorption and subsequent elution of a specific antibody or antigen using an immunosorbent containing the homologous antigen or antibody.
A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Changes in the amounts of various chemicals (neurotransmitters, receptors, enzymes, and other metabolites) specific to the area of the central nervous system contained within the head. These are monitored over time, during sensory stimulation, or under different disease states.
The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.
Protein domains that are enriched in PROLINE. The cyclical nature of proline causes the peptide bonds it forms to have a limited degree of conformational mobility. Therefore the presence of multiple prolines in close proximity to each other can convey a distinct conformational arrangement to a peptide chain.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Elements of limited time intervals, contributing to particular results or situations.
An enzyme that catalyzes the conversion of alpha D-glucose 1-phosphate to alpha D-glucose 6-phosphate. EC
Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
Proteins that bind to RNA molecules. Included here are RIBONUCLEOPROTEINS and other proteins whose function is to bind specifically to RNA.
Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.
A highly glycosylated and sulfated phosphoprotein that is found almost exclusively in mineralized connective tissues. It is an extracellular matrix protein that binds to hydroxyapatite through polyglutamic acid sequences and mediates cell attachment through an RGD sequence.
Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.
Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.
Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).
Ligand-binding assays that measure protein-protein, protein-small molecule, or protein-nucleic acid interactions using a very large set of capturing molecules, i.e., those attached separately on a solid support, to measure the presence or interaction of target molecules in the sample.
A PROTEIN-TYROSINE KINASE family that was originally identified by homology to the Rous sarcoma virus ONCOGENE PROTEIN PP60(V-SRC). They interact with a variety of cell-surface receptors and participate in intracellular signal transduction pathways. Oncogenic forms of src-family kinases can occur through altered regulation or expression of the endogenous protein and by virally encoded src (v-src) genes.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.
A heat-stable, low-molecular-weight activator protein found mainly in the brain and heart. The binding of calcium ions to this protein allows this protein to bind to cyclic nucleotide phosphodiesterases and to adenyl cyclase with subsequent activation. Thereby this protein modulates cyclic AMP and cyclic GMP levels.
A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.
Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.
Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.
A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).
Compounds that bind to and block the stimulation of PURINERGIC P2Y RECEPTORS. Included under this heading are antagonists for specific P2Y receptor subtypes.

Endocytosis: EH domains lend a hand. (1/18461)

A number of proteins that have been implicated in endocytosis feature a conserved protein-interaction module known as an EH domain. The three-dimensional structure of an EH domain has recently been solved, and is likely to presage significant advances in understanding molecular mechanisms of endocytosis.  (+info)

The hematopoietic-specific adaptor protein gads functions in T-cell signaling via interactions with the SLP-76 and LAT adaptors. (2/18461)

BACKGROUND: The adaptor protein Gads is a Grb2-related protein originally identified on the basis of its interaction with the tyrosine-phosphorylated form of the docking protein Shc. Gads protein expression is restricted to hematopoietic tissues and cell lines. Gads contains a Src homology 2 (SH2) domain, which has previously been shown to have a similar binding specificity to that of Grb2. Gads also possesses two SH3 domains, but these have a distinct binding specificity to those of Grb2, as Gads does not bind to known Grb2 SH3 domain targets. Here, we investigated whether Gads is involved in T-cell signaling. RESULTS: We found that Gads is highly expressed in T cells and that the SLP-76 adaptor protein is a major Gads-associated protein in vivo. The constitutive interaction between Gads and SLP-76 was mediated by the carboxy-terminal SH3 domain of Gads and a 20 amino-acid proline-rich region in SLP-76. Gads also coimmunoprecipitated the tyrosine-phosphorylated form of the linker for activated T cells (LAT) adaptor protein following cross-linking of the T-cell receptor; this interaction was mediated by the Gads SH2 domain. Overexpression of Gads and SLP-76 resulted in a synergistic augmentation of T-cell signaling, as measured by activation of nuclear factor of activated T cells (NFAT), and this cooperation required a functional Gads SH2 domain. CONCLUSIONS: These results demonstrate that Gads plays an important role in T-cell signaling via its association with SLP-76 and LAT. Gads may promote cross-talk between the LAT and SLP-76 signaling complexes, thereby coupling membrane-proximal events to downstream signaling pathways.  (+info)

The splicing factor-associated protein, p32, regulates RNA splicing by inhibiting ASF/SF2 RNA binding and phosphorylation. (3/18461)

The cellular protein p32 was isolated originally as a protein tightly associated with the essential splicing factor ASF/SF2 during its purification from HeLa cells. ASF/SF2 is a member of the SR family of splicing factors, which stimulate constitutive splicing and regulate alternative RNA splicing in a positive or negative fashion, depending on where on the pre-mRNA they bind. Here we present evidence that p32 interacts with ASF/SF2 and SRp30c, another member of the SR protein family. We further show that p32 inhibits ASF/SF2 function as both a splicing enhancer and splicing repressor protein by preventing stable ASF/SF2 interaction with RNA, but p32 does not block SRp30c function. ASF/SF2 is highly phosphorylated in vivo, a modification required for stable RNA binding and protein-protein interaction during spliceosome formation, and this phosphorylation, either through HeLa nuclear extracts or through specific SR protein kinases, is inhibited by p32. Our results suggest that p32 functions as an ASF/SF2 inhibitory factor, regulating ASF/SF2 RNA binding and phosphorylation. These findings place p32 into a new group of proteins that control RNA splicing by sequestering an essential RNA splicing factor into an inhibitory complex.  (+info)

The Gab1 PH domain is required for localization of Gab1 at sites of cell-cell contact and epithelial morphogenesis downstream from the met receptor tyrosine kinase. (4/18461)

Stimulation of the hepatocyte growth factor (HGF) receptor tyrosine kinase, Met, induces mitogenesis, motility, invasion, and branching tubulogenesis of epithelial and endothelial cell lines in culture. We have previously shown that Gab1 is the major phosphorylated protein following stimulation of the Met receptor in epithelial cells that undergo a morphogenic program in response to HGF. Gab1 is a member of the family of IRS-1-like multisubstrate docking proteins and, like IRS-1, contains an amino-terminal pleckstrin homology domain, in addition to multiple tyrosine residues that are potential binding sites for proteins that contain SH2 or PTB domains. Following stimulation of epithelial cells with HGF, Gab1 associates with phosphatidylinositol 3-kinase and the tyrosine phosphatase SHP2. Met receptor mutants that are impaired in their association with Gab1 fail to induce branching tubulogenesis. Overexpression of Gab1 rescues the Met-dependent tubulogenic response in these cell lines. The ability of Gab1 to promote tubulogenesis is dependent on its pleckstrin homology domain. Whereas the wild-type Gab1 protein is localized to areas of cell-cell contact, a Gab1 protein lacking the pleckstrin homology domain is localized predominantly in the cytoplasm. Localization of Gab1 to areas of cell-cell contact is inhibited by LY294002, demonstrating that phosphatidylinositol 3-kinase activity is required. These data show that Gab1 is an important mediator of branching tubulogenesis downstream from the Met receptor and identify phosphatidylinositol 3-kinase and the Gab1 pleckstrin homology domain as crucial for subcellular localization of Gab1 and biological responses.  (+info)

The histone acetylase PCAF is a phorbol-ester-inducible coactivator of the IRF family that confers enhanced interferon responsiveness. (5/18461)

Transcription factors of the interferon regulatory factor (IRF) family bind to the type I interferon (IFN)-responsive element (ISRE) and activate transcription from IFN-inducible genes. To identify cofactors that associate with IRF proteins, DNA affinity binding assays were performed with nuclear extracts prepared from tissue culture cells. The results demonstrated that the endogenous IRFs bound to the ISRE are complexed with the histone acetylases, PCAF, GCN5, and p300/CREB binding protein and that histone acetylase activities are accumulated on the IRF-ISRE complexes. By testing recombinant proteins, we show that PCAF directly binds to some but not all members of the IRF family through distinct domains of the two proteins. This interaction was functionally significant, since transfection of PCAF strongly enhanced IRF-1- and IRF-2-dependent promoter activities. Further studies showed that expression of PCAF and other histone acetylases was markedly induced in U937 cells upon phorbol ester treatment, which led to increased recruitment of PCAF to the IRF-ISRE complexes. Coinciding with the induction of histone acetylases, phorbol ester markedly enhanced IFN-alpha-stimulated gene expression in U937 cells. Supporting the role for PCAF in conferring IFN responsiveness, transfection of PCAF into U937 cells led to a large increase in IFN-alpha-inducible promoter activity. These results demonstrate that PCAF is a phorbol ester-inducible coactivator of the IRF proteins which contributes to the establishment of type I IFN responsiveness.  (+info)

Mutations of oncoprotein 18/stathmin identify tubulin-directed regulatory activities distinct from tubulin association. (6/18461)

Oncoprotein 18/stathmin (Op18) is a recently identified phosphorylation-responsive regulator of the microtubule (MT) system. It was originally proposed that Op18 specifically regulates dynamic properties of MTs by associating with tubulin, but it has subsequently been proposed that Op18 acts simply by sequestering of tubulin heterodimers. We have dissected the mechanistic action of Op18 by generation of two distinct classes of mutants. One class has interruptions of the heptad repeats of a potential coiled-coil region of Op18, and the other involves substitution at all four phosphorylation sites with negatively charged Glu residues. Both types of mutation result in Op18 proteins with a limited decrease in tubulin complex formation. However, the MT-destabilizing activities of the coiled-coil mutants are more severely reduced in transfected leukemia cells than those of the Glu-substituted Op18 derivative, providing evidence for tubulin-directed regulatory activities distinct from tubulin complex formation. Analysis of Op18-mediated regulation of tubulin GTPase activity and taxol-promoted tubulin polymerization showed that while wild-type and Glu-substituted Op18 derivatives are active, the coiled-coil mutants are essentially inactive. This suggests that Op18-tubulin contact involves structural motifs that deliver a signal of regulatory importance to the MT system.  (+info)

BLNK required for coupling Syk to PLC gamma 2 and Rac1-JNK in B cells. (7/18461)

Signaling through the B cell receptor (BCR) is essential for B cell function and development. Despite the key role of Syk in BCR signaling, little is known about the mechanism by which Syk transmits downstream effectors. BLNK (B cell LiNKer protein), a substrate for Syk, is now shown to be essential in activating phospholipase C (PLC)gamma 2 and JNK. The BCR-induced PLC gamma 2 activation, but not the JNK activation, was restored by introduction of PLC gamma 2 membrane-associated form into BLNK-deficient B cells. As JNK activation requires both Rac1 and PLC gamma 2, our results suggest that BLNK regulates the Rac1-JNK pathway, in addition to modulating PLC gamma 2 localization.  (+info)

Characterization and partial purification of a novel neutrophil membrane-associated kinase capable of phosphorylating the respiratory burst component p47phox. (8/18461)

The phosphorylation of p47phox is widely viewed as an important step in the activation of the neutrophil respiratory burst oxidase system. The exact nature of the kinase(s) responsible remains to be elucidated. We show here that such a kinase was detected on neutrophil membranes activated by either PMA or formyl-methionyl-leucyl-phenylalanine. This enzyme is not intrinsic to the neutrophil membrane and could be eluted with 0.5 M NaCl. The kinase activity was partially purified and was found not to be due to the presence of previously suggested kinases, including protein kinase C isotypes, mitogen-activated protein kinase and protein kinase B. Gel filtration and renaturation in substrate gels suggest a molecular mass of between 45 and 51 kDa. The kinase activity was independent of calcium and lipids but was potently inhibited by staurosporine. Treatment with protein phosphatase 2Ac suggested that the kinase was activated by serine/threonine phosphorylation. Phosphopeptide maps indicated that the kinase phosphorylated p47phox on similar sites to those found in vivo. These results indicate that activation of neutrophils by PMA results in the activation of a membrane-associated kinase that may play a part in the regulation of neutrophil NADPH oxidase through its ability to phosphorylate p47phox.  (+info)

Effects of miR-34a on cellular phosphoprotein activation in NB1691luc cells. NB1691luc (1 × 106) cells were reverse transfected with premiR-34a (30 μM) or a p
A 47 kDa phosphoprotein is involved in the respiratory-burst oxidase of phagocytic cells. After stimulation of neutrophils with phorbol myristate acetate, this phosphoprotein was identified in both the cytosol and membranes. Peptide mapping of the two forms resulted in identical patterns of phosphopeptides. Dose-response curves for accumulation of phosphoprotein in the two sites were very similar, whereas the detection of the phosphoprotein in the cytosol preceded that in the membranes. The membrane-associated 47 kDa phosphoprotein was absent from the neutrophils of patients with X-chromosome-linked chronic granulomatous disease, which lack cytochrome b-245, and intermediate levels were detected in the membranes of their heterozygote carrier mothers. Activation of the neutrophil oxidase system appears to be dependent upon phosphorylation of the cytosolic 47 kDa protein and its association with cytochrome b-245 in the membranes. It is probably the cytosolic factor required for reconstitution of ...
TY - JOUR. T1 - Cloning of a novel phosphoprotein regulated by colony-stimulating factor 1 shares a domain with the Drosophila disabled gene product. AU - Xu, Xiangxi. AU - Yang, W.. AU - Jackowski, S.. AU - Rock, C. O.. PY - 1995/1/1. Y1 - 1995/1/1. N2 - A unique protein with an apparent molecular mass of 96 kilodaltons (p96) was detected in the routine macrophage cell line, BAC1.2F5. The murine cDNA encoding p96 was cloned and sequenced, along with cDNAs representing two alternatively spliced forms of the protein. All three proteins possessed identical amino-terminal domains with significant similarity to the amino- terminal domain of the Drosophila disabled gene product and carboxyl-terminal domains containing proline-rich sequences characteristic of src homology region (domain 3) binding regions. BAC1.2F5 cells predominately expressed the p96 protein, although mRNA and protein corresponding to the p67 splice variant were also detected. Electrophoretic gel retardation of p96 in response to ...
The Phosphoprotein Enrichment Kit provides a rapid and specific IMAC-based procedure for isolating phosphorylated proteins from mammalian cells and tissues. The Phosphoprotein Enrichment Kit Procedure is fast, with an average cell-to-sample purification time of less than 2 hours. It is also straightforward, consisting of four main steps (Figure 1): adding Extraction/Loading Buffer to the cell or tissue pellet to extract total cellular protein, loading the extract on an affinity column, washing, and, finally, eluting the bound phosphoprotein with the detergent-free Elution Buffer. A single buffer-Extraction/Loading Buffer-is used for both the protein extraction and affinity column steps, making buffer exchange unnecessary. This saves time and prevents sample loss. The procedure is non-denaturing, so phosphoproteins remain folded throughout the process, even during the extraction and elution steps.. The Phosphoprotein Enrichment Kit may be used with any mammalian cell type. Cell lines tested ...
The two proteins with different electrophoretic mobilities that were immunodetected by using the anti-FUS5 antibody in both the wild type and cop1-6 are products of the same gene. If these two proteins were the products of two different genes, we would expect that only one of them would be missing in the fus5 mutant strains, but this is not the case. Both proteins are absent in all fus5 mutant strains, although both are present in strains known to have an intact COP9 complex, such as wild type and cop1-6. In our gel filtration analysis, the FUS5 doublet was also detected in fractions corresponding to the COP9 complex. However, we do not know whether these two proteins represent different functional forms of FUS5, or whether they are simply an artifact of SDS-PAGE. It should be noted that both FUS6 and COP9 sometimes appear as a doublet after SDS-PAGE (Chamovitz et al., 1996; Chamovitz and Deng, 1998).. The mobility shift of FUS5 between that of the wild type and the cop9 and fus6 mutants is most ...
Intracellular protein levels, subcellular localization, or activation state are reflective of a cells functions. Some relevant cell populations are so rare as...
The KOMP Repository is located at the University of California Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...
Homo sapiens acidic (leucine-rich) nuclear phosphoprotein 32 family, member A (ANP32A), mRNA. (H00008125-R01) - Products - Abnova
Activation of cytoplasmic tyrosine kinase activity is required for T cell receptor (TCR)-dependent lymphocyte activation (1). Adapter proteins serve as substrates for these kinases and as such may function to couple the TCR with downstream signaling events (2-6). SLP-76 is a hematopoietic cell-specific adapter protein that is phosphorylated rapidly on NH2-terminal tyrosine residues after TCR ligation (3), providing a binding site for the Src homology 2 (SH2) domain of Vav (7). SLP-76 also contains a central proline-rich region that associates constitutively with the SH3 domains of Grb2 (8). In addition, SLP-76 has a COOH-terminal SH2 domain that inducibly associates with SLAP-130 (SLP-76-associated phosphoprotein of 130 kD) and an unidentified 62-kD tyrosine phosphoprotein (5, 8, 9). The ability of SLP-76 to augment TCR-dependent nuclear factor of activated T cells (NFAT) activation when transiently overexpressed in a T cell line is dependent on the presence of each of these domains, suggesting ...
Shop Steroidogenic acute regulatory protein ELISA Kit, Recombinant Protein and Steroidogenic acute regulatory protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Cytokine signal transduction is essential for normal immune function and controls the quality of responses to a wide variety of microbial infections. Innate and adaptive host responses to virus infections are regulated by autocrine and paracrine cytokine signaling systems. For most cytokines, receptor binding triggers an intracellular signaling cascade involving one or more signal transducer and activator of transcription (STAT) proteins. Diverse cytokine and growth factor signaling pathways produce active STAT transcription factors that specify mRNA induction profiles (26). For example, the alpha and beta interferon (IFN-α/β) family is of primary importance for both innate and adaptive antiviral immunity (reviewed in references 1, 49, and 53). In the innate antiviral system, IFN-α/β initiates a receptor-mediated signaling system that produces an activated STAT1-STAT2-IRF9 heterotrimeric transcription complex known as ISGF3 (27). The ISGF3 complex translocates to the nucleus, where it can ...
Tang L.-Y., Deng N., Wang L.-S., Dai J., Wang Z.-L., Jiang X.-S., Li S.-J., Li L., Sheng Q.-H., Wu D.-Q., Li L., Zeng R.. The complexity of canonical Wnt signaling comes not only from the numerous components but also from multiple post-translational modifications. Protein phosphorylation is one of the most common modifications that propagates signals from extracellular stimuli to downstream effectors. To investigate the global phosphorylation regulation and uncover novel phosphoproteins at the early stages of canonical Wnt signaling, HEK293 cells were metabolically labeled with two stable isotopic forms of lysine and were stimulated for 0, 1, or 30 min with purified Wnt3a. After phosphoprotein enrichment and LC-MS/MS analysis, 1057 proteins were identified in all three time points. In total 287 proteins showed a 1.5-fold or greater change in at least one time point. In addition to many known Wnt signaling transducers, other phosphoproteins were identified and quantitated, implicating their ...
ARPP21 overexpression lysate, 0.1 mg. Transient overexpression lysate of cyclic AMP-regulated phosphoprotein, 21 kD (ARPP-21), transcript variant 4
Procaspase-8, the zymogen type of the apoptosis-initiator caspase-8, undergoes phosphorylation following integrin-mediated cell connection to an extracellular matrix base. CrkII and Crk, each bearing an Src-homology 2 domains (SH2) and one or two Src homology 3 (SH3) websites, respectively. CrkL (and knockouts display cardiac and sensory crest flaws, ending in embryonic lethality.17,18 Here, we offer proof that caspase-8 interacts with the You will need2 domains of CrkL in a Src- and adhesion-dependent way, and that this connections stimulates cellular migration. Outcomes Caspase-8 interacts with CrkL SH2 domains We observed the de novo phosphorylation of many protein, in caspase-8 showing cells selectively, pursuing cell adhesion to fibronectin substrates. These included a phosphoprotein at ~37 kDa (Fig.?1A). To determine whether the phosphoprotein may end up being component of a complicated linked with the caspase, caspase-8 immunoprecipitations had been performed by us, solved the necessary ...
SLP76 (SH2 domain-containing leukocyte protein of 76 kDa) is a cytosolic adaptor protein which translocates to the plasma mambrane and is involved in multiple signaling pathways in T cells, mast cells, neutrophils and platelets; B cells express its analog SLP65/BLNK (B cell linker protein). SLP76 is phosphorylated by Syk-family and Tec-family tyrosine kinases and couples them to the phosphorylation and activation of PLC-gamma. Via Gads or Grb2, SLP76 also associates with LAT adaptor by involvement of SLP76 proline-rich region. The SH2 domain of SLP76 has been identified as the region involved in binding the serine/threonine kinase HPK1. HPK1 may act as both a positive and a negative regulator by promoting the Jnk-mitogen activated protein kinase (MAPK) pathway and inhibiting the pathway leading to AP-1 activation ...
RecName: Full=Ezrin-radixin-moesin-binding phosphoprotein 50; Short=EBP50; AltName: Full=Na(+)/H(+) exchange regulatory cofactor NHE-RF; AltName: Full=NHERF-1; AltName: Full=Regulatory cofactor of Na(+)/H(+) exchanger; AltName: Full=Sodium-hydrogen exchanger regulatory factor 1; AltName: Full=Solute carrier family 9 isoform A3 regulatory factor 1 ...
The adaptor protein Src homology 2 domain-containing leukocyte phosphoprotein of 76 kDa (SLP-76) plays a crucial role in T cell activation by linking antigen receptor (T cell receptor, TCR) signals to downstream pathways ...
Protein phosphorylation affects most, if not all, cellular activities in eukaryotes and is essential for cell proliferation and development. An estimated 30% of cellular proteins are phosphorylated, representing the phosphoproteome, and phosphorylation can alter a proteins function, activity, local …
TP53 / p53 (C-Terminus) antibody | P04637 | Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53
Stimulation of beta2-adrenergic receptors on the cell surface by adrenaline or noradrenaline leads to alterations in the metabolism, excitability, differentiation and growth of many cell types. These effects have traditionally been thought to be mediated exclusively by receptor activation of intrace …
VASP - VASP (untagged)-Human vasodilator-stimulated phosphoprotein (VASP) available for purchase from OriGene - Your Gene Company.
This gene encodes a member of the paralemmin protein family. The product of this gene is a prenylated and palmitoylated phosphoprotein that associates with the cytoplasmic face of plasma membranes and is implicated in plasma membrane dynamics in neurons and other cell types. Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008 ...
VASP (phospho Ser239) antibody (vasodilator-stimulated phosphoprotein) for IHC-P, WB. Anti-VASP (phospho Ser239) pAb (GTX38661) is tested in Human, Mouse samples. 100% Ab-Assurance.
Protein phosphorylation significantly impacts protein function. Find products, protocols, articles and pathways for protein phosphorylation research.
The Vasp Tetramerization Domain is a Right-Handed Coiled Coil Based on a 15-Residue Repeat.. PubMed ID: 15-569-942. From NIHs 3D Print Exchange. ...
GO:0072332. A series of molecular signals in which an intracellular signal is conveyed to trigger the apoptotic death of a cell. The pathway is induced by the cell cycle regulator phosphoprotein p53, or an equivalent protein, and ends when the execution phase of apoptosis is triggered. ...
This overview provides a history of protein phosphorylation research and provides the reader with an understanding of how and why labeling studies are performed
Phosphoflow analysis of phosphorylated proteins allows researchers a quick and effective way to measure signalling cascades in individual cells.
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Hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is a prominent substrate for activated tyrosine kinase receptors that has been proposed to play a role in endosomal membrane trafficking. The protein contains a FYVE domain, which specifically binds to the lipid phosphatidylinositol (PI) 3-phosphate (PI 3-P). We show that this interaction is required both for correct localization of the protein to endosomes that only partially coincides with early endosomal autoantigen 1 and for efficient tyrosine phosphorylation of the protein in response to epidermal growth factor stimulation. Treatment with wortmannin reveals that Hrs phosphorylation also requires PI 3-kinase activity, which is necessary to generate the PI 3-P required for localization. We have used both hypertonic media and expression of a dominant-negative form of dynamin (K44A) to inhibit endocytosis; under which conditions, receptor stimulation fails to elicit phosphorylation of Hrs. Our results provide a clear example of the
Immune responses are initiated when molecules of microbial origin are sensed by the Toll-like receptors (TLRs). We now report the identification of essential molecular components for the trafficking of the lipopolysaccharide (LPS) receptor complex. LPS was endocytosed by a receptor-mediated mechanism dependent on dynamin and clathrin and colocalized with TLR4 on early/sorting endosomes. TLR4 was ubiquitinated and associated with the ubiquitin-binding endosomal sorting protein hepatocyte growth factor-regulated tyrosine kinase substrate, Hrs. Inhibition of endocytosis and endosomal sorting increased LPS signaling. Finally, the LPS receptor complex was sorted to late endosomes/lysosomes for degradation and loading of associated antigens onto HLA class II molecules for presentation to CD4+ T cells. Our results show that endosomal trafficking of the LPS receptor complex is essential for signal termination and LPS-associated antigen presentation, thus controlling both innate and adaptive immunity through
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Abstract. RPLP1 is one of acidic ribosomal phosphoproteins encoded by RPLP1 gene, which plays an important role in the elongation step of protein synthesis. The cDNA of RPLP1 was cloned successfully for the first time from the Giant Panda (Ailuropoda melanoleuca) using RT-PCR technology, which was also sequenced, analyzed preliminarily and expressed in E.coli. The cDNA fragment cloned is 449bp in size, containing an open reading frame of 344bp encoding 114 amino acids. Alignment analysis indicated that the nucleotide sequence and the deduced amino acid sequence are highly conserved to other five species studied, including Homo sapiens, Mus musculus, Rattus norvegicus, Bos Taurus and Sus scrofa. The homologies for nucleotide sequences of Giant Panda PPLP1 to that of these species are 92.4%, 89.8%, 89.0%, 91.3% and 87.5%, while the homologies for amino acid sequences are 96.5%, 94.7%, 95.6%, 96.5% and 88.6%. Topology prediction showed there are three Casein kinase II phosphorylation sites and two ...
ABSTRACT: The Insulin Receptor Substrate (IRS) proteins are cytoplasmic adaptor proteins that function as essential signaling intermediates downstream of activated cell surface receptors, many of which have been implicated in cancer. The IRS proteins do not contain any intrinsic kinase activity, but rather serve as scaffolds to organize signaling complexes and initiate intracellular signaling pathways. As common intermediates of multiple receptors that can influence tumor progression, the IRS proteins are positioned to play a pivotal role in regulating the response of tumor cells to many different microenvironmental stimuli. Limited studies on IRS expression in human tumors and studies on IRS function in human tumor cell lines and in mouse models have provided clues to the potential function of these adaptor proteins in human cancer. A general theme arises from these studies; IRS-1 and IRS-4 are most often associated with tumor growth and proliferation and IRS-2 is most often associated with tumor
This protein, p55 , of a 55-kD erythrocyte membrane protein locus Xq28: [§§]; exon sizes range from 69 (exon 5) to 203 (exon 10) bp, is the prototype of a family of membrane-associated proteins that contains three distinct domains in its primary structure: estimates relative utilization of the three sites the binding sites for band 3. The interactions involving protein 4.1 with p55 and p55 with GPC/D that migrate in the region of band 4.9 in a directional fashion are of high affinity*(nM) termed MAGUKs (membrane-associated guanylate kinase homologs) with the FERM domain of protein 4.1R is the most extensively palmitoylated protein of the erythrocyte membrane a classical PDZ domain-to-PDZ binding motif (PBM) mechanism also designated as MPP1 FERM domain of NF2 protein. Human erythroid p55, a palmitoylated peripheral membrane phosphoprotein were used to map the protein 4.1 binding site on human erythroid glycophorin C, a transmembrane protein of red blood cells phosphorylation to the cell ...
Cloning, Expression and Hormonal Regulation of Steroidogenic Acute Regulatory Protein Gene in Buffalo Ovary - StAR Gene;StAR mRNA;Semi-quantitative RT-PCR;Granulosa Cells;Ovary;Buffalo;
Protein target information for Dentin matrix acidic phosphoprotein 1 (pig). Find diseases associated with this biological target and compounds tested against it in bioassay experiments.
In eukaryotes, hundreds of protein kinases (PKs) specifically and precisely modify thousands of substrates at specific amino acid residues to faithfully orchestrate numerous biological processes, and reversibly determine the cellular dynamics and plasticity. Although over 100,000 phosphorylation sites (p-sites) have been experimentally identified from phosphoproteomic studies, the regulatory PKs for most of these sites still remain to be characterized. Here, we present a novel software package of iGPS for the prediction of in vivo site-specific kinase-substrate relations mainly from the phosphoproteomic data. By critical evaluations and comparisons, the performance of iGPS is satisfying and better than other existed tools. Based on the prediction results, we modeled protein phosphorylation networks and observed that the eukaryotic phospho-regulation is poorly conserved at the site and substrate levels. With an integrative procedure, we conducted a large-scale phosphorylation analysis of human ...
Characterization of the Borna disease virus phosphoprotein, p23.: Borna disease virus infection is diagnosed by the presence of serum antibodies reactive with t
The polymerization-inducing activity of the p48 integral membrane protein does not require an intact bilayer, and so we were able to harvest vesicles from S100, solubilize the membrane proteins with detergents, separate them by size exclusion chromatography and recombine fractions at each step with cytosol to assay for filament formation. The requirement for tyrosine phosphorylation provided a convenient, complementary biochemical assay. At each step in fractionation, we found that fractions that induced MSP polymerization in cytosol also contained phosphorylated p48 on western blots. In most preparations of vesicles, p48 was the only protein that labeled with antiphosphotyrosine. Some preparations also contained a minor reactive band at Mr ∼68 kDa. The sequence of a 20 amino acid peptide fragment of this protein matched that of flavoprotein subunit II of fumarate reductase, a 67.9 kDa mitochondrial enzyme from Ascaris that contains phosphotyrosine residues (Kuramochi et al., 1994). Thus, the ...
Complex brain functions, such as learning and memory, are believed to involve changes in the efficiency of communication between nerve cells. Therefore, the elucidation of the molecular mechanisms that regulate synaptic transmission, the process of intercellular communication, is an essential step toward understanding nervous system function. Several proteins associated with synaptic vesicles, the organelles that store neurotransmitters, are targets for protein phosphorylation and dephosphorylation. One of these phosphoproteins, synapsin I, by means of changes in its state of phosphorylation, appears to control the fraction of synaptic vesicles available for release and thereby to regulate the efficiency of neurotransmitter release. This article describes current understanding of the mechanism by which synapsin I modulates communication between nerve cells and reviews the properties and putative functions of other phosphoproteins associated with synaptic vesicles. ...
Primary Objective 1: Determine changes in tumor EGFR, pEGFR, downstream signaling and novel phosphoproteins following a loading dose of cetuximab in patients who are poor candidates for chemoradiation (age =70 years or with significant co-morbidities) and are therefore treated with cetuximab with radiation.. Primary Objective 2: Characterize clinical outcomes, including local recurrence, progression-free survival and overall survival in these patients, and correlate these clinical outcomes with the changes in tumor EGFR, pEGFR, downstream signaling, and novel phosphoproteins.. Primary Objective 3: Describe the toxicity, in particular mucositis/dysphagia, of this regimen.. Secondary Objective 1: Conduct normal mucosa EGFR assessment for comparison with tumor sample.. Secondary Objective 2: Correlate HPV presence and titer with p53 status and clinical outcome. ...
Two-dimensional gel electrophoresis of nuclear phosphoproteins of Novikoff hepatoma and regenerating liver.: Two-dimensional polyacrylamide gel electrophoresis
Integrins interact with extracellular matrix (ECM) and deliver intracellular signaling for cell proliferation, survival, and motility. During tumor metastasis, integrin-mediated cell adhesion to and migration on the ECM proteins are required for cancer cell survival and adaptation to the new microenvironment. Using stable isotope labeling by amino acids in cell culture-mass spectrometry, we profiled the phosphoproteomic changes induced by the interactions of cell integrins with type I collagen, the most common ECM substratum. Integrin-ECM interactions modulate phosphorylation of 517 serine, threonine, or tyrosine residues in 513 peptides, corresponding to 357 proteins. Among these proteins, 33 key signaling mediators with kinase or phosphatase activity were subjected to small interfering RNA-based functional screening. Three integrin-regulated kinases, DBF4, PAK2, and GRK6, were identified for their critical role in cell adhesion and migration possibly through their regulation of actin ...
T cells play a central role in immune responses mounted against pathogens and cancer. Dramatic cytoskeletal reorganization during T cell activation is a prerequisite for a specific immune response (for references see Penninger and Crabtree 1999). Upon binding to an antigen-presenting cell (APC), the cytoskeleton of a T cell rapidly polarizes. The accumulation of actin in a tight collar at the T cell-APC interface is thought to stabilize a continuous contact between T cells and APCs (Ryser et al. 1982; Valitutti et al. 1995). The formation of this tight contact is accompanied by the reorientation of the microtubule-organizing center towards the contact site to ensure a polarized release of cytokines or cytotoxic factors (Geiger et al. 1982; Kupfer et al. 1987, Kupfer et al. 1991). Although actin remodeling is thought to be essential for T cell activation (see Penninger and Crabtree 1999), it is not known how T cell receptor (TCR) signaling is linked to the rearrangement of the actin ...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein with a C-terminal Sprouty-like cysteine-rich domain (SRY) and an N-terminal Ena/Vasodilator-stimulated phosphoprotein (VASP) homology-1 (EVH-1) domain. The encoded protein is a member of a family of proteins that negatively regulates mitogen-activated protein (MAP) kinase signaling, particularly during organogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012 ...
The KOMP Repository is located at the University of California Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...
Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5 ...
Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5 ...
(2013) Schokoroy et al. PLoS ONE. Background:The ErbB receptors, Ras proteins and nucleolin are major contributors to malignant transformation. The pleiotropic protein nucleolin can bind to both Ras protein and ErbB receptors. Previously, we have demonstrated a crosstalk between Ras, nucleolin an...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
c-Myc, 0.1 ml. c-Myc is a multifunctiol, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation.
The BRCA-1 antibody gene codes for a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor.
Tyrosine, 3-isobutyl-1-methylxanthine, Antibodies, Cells, Dbcamp, Ibmx, Immunoblotting, Kinase, Phosphoproteins, Phosphorylation, Proteins, Serine, Sperm, Threonine, Time
购买我们的小鼠Paxillin (phospho S83)肽。ab42724可作为ab31833的封闭肽并经过Blocking, ELISA实验验证。Abcam提供免费的实验方案,操作技巧及专业的支持。
Phosphoproteins have been proposed as biomarkers for breast cancer. Protein phosphorylation Keyword - Phosphoprotein ... Liquid Biopsy Could Get Boost from Phosphoproteins. 2017 v t e (Phosphoproteins, All stub articles, Protein stubs). ... A phosphoprotein is a protein that is posttranslationally modified by the attachment of either a single phosphate group, or a ... Phosphoproteins in extracellular vesicles as candidate markers for breast cancer Cozzone AJ (1988). "Protein phosphorylation in ...
... , or phosphophoryn, is one of three proteins formed from dentin sialophosphoprotein and is important in ... Being highly negative, dentin phosphoprotein is able to attract large amounts of calcium. In vitro studies also indicate ...
... (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an N- ... Vasodilator-stimulated phosphoprotein has been shown to interact with Zyxin, Profilin 1, and PFN2. GRCh38: Ensembl release 89: ... Vasodilator-stimulated phosphoprotein is a protein that in humans is encoded by the VASP gene. ... 1992). "The 46/50 kDa phosphoprotein VASP purified from human platelets is a novel protein associated with actin filaments and ...
... is a protein that in humans is encoded by the NOLC1 gene. Nucleolar phosphoprotein p130 has been ... "Entrez Gene: NOLC1 nucleolar and coiled-body phosphoprotein 1". Isaac, C; Yang Y; Meier U T (Jul 1998). "Nopp140 functions as a ... Chen HK, Yeh NH (1997). "The nucleolar phosphoprotein P130 is a GTPase/ATPase with intrinsic property to form large complexes ... Miau LH, Chang CJ, Tsai WH, Lee SC (1997). "Identification and characterization of a nucleolar phosphoprotein, Nopp140, as a ...
The translocated actin-recruiting phosphoprotein (Tarp) is a protein that may mediate the invasion of epithelial cells by ... "Translocated actin-recruiting phosphoprotein [Chlamydia trachomatis] - Protein - NCBI". v t e (Protein pages needing a picture ...
... is a protein that in humans is encoded by the ANP32A gene. It is ... Acidic leucine-rich nuclear phosphoprotein 32 family member A has been shown to interact with MAP1B, TAF1A and Protein SET. ... "Entrez Gene: ANP32A Acidic (leucine-rich) nuclear phosphoprotein 32 family, member A". Opal, Puneet; Garcia Jesus J; Propst ...
Phosphoprotein. Res. Advances in Second Messenger and Phosphoprotein Research. 31: 105-11. doi:10.1016/S1040-7952(97)80012-2. ...
"Systematic Identification of Mitotic Phosphoproteins". Current Biology. 7 (5): 338-48. doi:10.1016/s0960-9822(06)00157-6. PMID ...
The genome encodes five proteins: matrix protein M; transmembrane glycoprotein G; nucleoprotein N; phosphoprotein P; and the ...
This protein acts as an enzyme that catalyzes the following reaction: ATP + a protein -> ADP + a phosphoprotein. MYLK4 is also ...
Gerber, Isak B.; Laukens, Kris; Witters, Erwin; Dubery, Ian A. (2006). "Lipopolysaccharide-responsive phosphoproteins in ... "Quantitative detection of phosphoproteins by combination of two-dimensional difference gel electrophoresis and phosphospecific ...
Gafken PR, Lampe PD (2006). "Methodologies for characterizing phosphoproteins by mass spectrometry". Cell Communication & ...
Gafken PR, Lampe PD (2006). "Methodologies for characterizing phosphoproteins by mass spectrometry". Cell Commun. Adhes. 13 (5- ...
It is a phosphoprotein. Vitellin is a generic name for major of many yolk proteins. Vitellin has been known since the 1930s. ... This phosphoprotein acts as a membrane, 1-3.5μm, that encloses the egg that comprises at least five glycoproteins that resemble ... https://doi.org/10.1016/0304-4165(86)90267-9 v t e (Use dmy dates from August 2018, Avian proteins, Phosphoproteins, All stub ...
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. Bibcode: ...
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ...
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. Bibcode: ...
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ...
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ...
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ...
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ...
August 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of ...
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ...
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. Bibcode: ...
August 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of ...
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. Bibcode: ...
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ...
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ...
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ...
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ...
odontogenesis associated phosphoproteinprovided by HGNC. Primary source. HGNC:HGNC:26300 See related. Ensembl:ENSG00000174792 ... This gene is thought to encode an extracellular matrix acidic phosphoprotein that has a function in enamel mineralization ... NM_001206981.2 → NP_001193910.1 odontogenesis associated phosphoprotein isoform 1 precursor. See identical proteins and their ... NM_001257072.2 → NP_001244001.1 odontogenesis associated phosphoprotein isoform 3 precursor. See identical proteins and their ...
Lettuce Necrotic Yellow Virus Phosphoprotein C-Terminal Domain ... Lettuce Necrotic Yellow Virus Phosphoprotein C-Terminal Domain ... Phosphoprotein. A. 81. Lettuce necrotic yellows virus isolate 318. Mutation(s): 0 Gene Names: P. ... Structure of the C-terminal domain of lettuce necrotic yellows virus phosphoprotein.. Martinez, N., Ribeiro, E.A., Leyrat, C., ... we localized a folded C-terminal domain in the amino acid sequence of its phosphoprotein. This domain consists of an autonomous ...
ARPP-21 is a cyclic AMP-regulated phosphoprotein of M(r) 21 kDa that is enriched in the cell bodies and terminals of medium- ... ARPP-21 is a cyclic AMP-regulated phosphoprotein of M(r) 21 kDa that is enriched in the cell bodies and terminals of medium- ... Drugs of abuse modulate the phosphorylation of ARPP-21, a cyclic AMP-regulated phosphoprotein enriched in the basal ganglia ...
View mouse Anp32e Chr3:95836557-95854699 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
Recombinant Dentin Matrix Acidic phosphoprotein 1 (DMP1) Protein (His tag). Spezies: Human. Quelle: Human Cells. Jetzt Produkt ... DMP1 (Dentin Matrix Acidic phosphoprotein 1 (DMP1)) Andere Bezeichnung Dentin Matrix Acidic Phosphoprotein 1 (DMP1 Produkte) ... Dentin Matrix Acidic phosphoprotein 1 (DMP1) (AA 17-489) protein (His tag) DMP1 Spezies: Ratte Wirt: Hefe Recombinant > 90 % ... Dentin Matrix Acidic phosphoprotein 1 (DMP1) (AA 17-510) protein (His tag) DMP1 Spezies: Rind (Kuh) Wirt: Hefe Recombinant > 90 ...
LS Protein Expression Solutions Competent Cells for Routine Protein Expression Phosphoprotein Generation Products (1). ... Competent cell lines for inducible phosphoprotein expression TKB1 Competent Cells are derived from the BL21... ...
Want to preserve phospho-proteins for Western blot and Antibody array protocols?. Phospho-Sure™ RTD™ Neuronal Cell & Soft ... Phospho-proteins can be easily monitored by using phospho-specific antibodies in various types of immuno-assays (Western blot, ... 3 tips to preserve phospho-proteins with home-made lysis buffers. As soon as the lysis buffer is added on samples, these are ... 1 alternative: ready-to-dilute non-toxic lysis buffer optimized for phospho-protein isolation. Comparison of the signal ...
Wyttenbach, A. and Tolkovsky, A.M. (2006) Differential phosphoprotein labeling (DIPPL), a method for comparing live cell ... Differential phosphoprotein labeling (DIPPL), a method for comparing live cell phosphoproteomes using simultaneous analysis of ... Differential phosphoprotein labeling (DIPPL), a method for comparing live cell phosphoproteomes using simultaneous analysis of ... Differential phosphoprotein labeling (DIPPL), a method for comparing live cell phosphoproteomes using simultaneous analysis of ...
gene and the phosphoprotein. (P). gene of. PVM. and attempted to. characterise the polypeptide products expressed from the N. ... Barr, John Nicholas (1993) Expression of the nucleoprotein and phosphoprotein genes of pneumonia virus of mice and specific ... Expression of the nucleoprotein and phosphoprotein genes of pneumonia virus of mice and specific interactions of the gene ... Nucleoproteins , Phosphoproteins, Viral genetics, Viral pneumonia , Pneumonia, Virus diseases -- Research, Genetics -- Research ...
The psbB gene is located 562 bp upstream of the psbH gene for the 10 kDa phosphoprotein of photosystem II. A small open reading ... The psbB gene is located 562 bp upstream of the psbH gene for the 10 kDa phosphoprotein of photosystem II. A small open reading ... The psbB gene is located 562 bp upstream of the psbH gene for the 10 kDa phosphoprotein of photosystem II. A small open reading ... The psbB gene is located 562 bp upstream of the psbH gene for the 10 kDa phosphoprotein of photosystem II. A small open reading ...
Identification of a calcium-regulated insulinoma cell phosphoprotein as an islet cell keratin. U K Schubart, U K Schubart ... U K Schubart, K L Fields; Identification of a calcium-regulated insulinoma cell phosphoprotein as an islet cell keratin.. J ...
Increased circulating cell signalling phosphoproteins in sera are useful forthe detection of pancreatic cancer.. Author Takano ... Increased circulating cell signalling phosphoproteins in sera are useful forthe detection of pancreatic cancer. ...
Mutations of MAP1B encoding a microtubule-associated phosphoprotein cause sensorineural hearing loss. ... Mutations of MAP1B encoding a microtubule-associated phosphoprotein cause sensorineural hearing loss. ...
Human Secreted Phosphoprotein 2 (SPP2) ELISA Kit, Cat#EKU07225. Write a Review Write a Review. × ... Human Secreted Phosphoprotein 2 (SPP2) ELISA Kit, Cat#EKU07225. Rating Required Select Rating. 1 star (worst). 2 stars. 3 stars ... This assay has high sensitivity and excellent specificity for detection of Secreted Phosphoprotein 2 (SPP2). No significant ... cross-reactivity or interference between Secreted Phosphoprotein 2 (SPP2) and analogues was observed. ...
N2 - The gene encoding p53 phosphoprotein, originally believed to be an oncogene, recently has been proposed as a candidate ... AB - The gene encoding p53 phosphoprotein, originally believed to be an oncogene, recently has been proposed as a candidate ... The gene encoding p53 phosphoprotein, originally believed to be an oncogene, recently has been proposed as a candidate ... abstract = "The gene encoding p53 phosphoprotein, originally believed to be an oncogene, recently has been proposed as a ...
phosphoprotein 41. *phosphoprotein 75. *SP110_HUMAN. *speckeled, 110-KD. *transcriptional coactivator Sp110 ...
Research Project 3 - Multiparameter Nanoparticle Detection of Phosphoproteins. (G. Nolan Project Leader) ... This project will utilize raman spectroscopy and nanoparticles for multiplexed detection of phosphoproteins. Subcellular ...
Combined quantification of intracellular (phospho-)proteins and transcriptomics from fixed single cells *Jan. P. Gerlach ...
SCAP1, SKAP55, pp55, Src Family Associated Phosphoprotein 1, Src kinase-associated phosphoprotein of 55 kDa, SCAP1 protein, ... Src kinase-associated phosphoprotein of 55 kDa protein, Protein Src kinase-associated phosphoprotein of 55 kDa, Rat Src kinase- ... associated phosphoprotein of 55 kDa Protein, Rat Src kinase-associated phosphoprotein of 55 kDa, Rat protein Src kinase- ... Src Family Associated Phosphoprotein 1 protein, Protein Src Family Associated Phosphoprotein 1, Rat Src Family Associated ...
In this light, actin-binding vasodilator-stimulated phosphoprotein (VASP) is a critical regulator of adherent cell migration, a ... Holt, Andrew W. Vasodilator-stimulated phosphoprotein regulates arterial smooth muscle cell migration. Doctoral Dissertation. ... Holt, Andrew W. (September 2016). Vasodilator-stimulated phosphoprotein regulates arterial smooth muscle cell migration ( ... Holt, Andrew W. Vasodilator-stimulated phosphoprotein regulates arterial smooth muscle cell migration [Doctoral Dissertation]. ...
Phosphoprotein. P. 2347-3072. 2348-3073. M. 3253-4210. 3254-4208. Matrix protein. M. 3262-4032. 3263-4033. ...
Measles virus phosphoprotein inhibits apoptosis and enhances clonogenic and migratory properties in HeLa cells. Journal of ... Measles virus phosphoprotein inhibits apoptosis and enhances clonogenic and migratory properties in HeLa cells. ... Two majorproteins, coded by the viral genome, are nucleocapsid protein (N) and phosphoprotein (P). The N protein protects the ...
enables phosphoprotein phosphatase activity IEA Inferred from Electronic Annotation. more info. enables protein serine/ ...
Mouse Src kinase-associated phosphoprotein 2 (Skap2) datasheet and description hight quality product and Backed by our ... src family associated phosphoprotein 2, src family-associated phosphoprotein 2, src kinase-associated phosphoprotein 2, src ... Src kinase-associated phosphoprotein 2, Src kinase-associated phosphoprotein 55-related protein, Src-associated adapter protein ... Recombinant Mouse Src kinase-associated phosphoprotein 2 (Skap2). Alternative names: Pyk2/RAFTK-associated protein, SKAP-HOM, ...
Enrichment and analysis of intact phosphoproteins in arabidopsis seedlings ... Enrichment of intact phosphoproteins prior to 2-DE and LC-MS/MS appears to enhance detection of phosphorylated threonine and ... phosphoprotein; ribulosebisphosphate carboxylase; threonine; tyrosine; Arabidopsis thaliana; carbohydrate metabolism; ... Enrichment and analysis of intact phosphoproteins in arabidopsis seedlings. From National Research Council Canada ...
Phospho-Proteins (464) Postsynaptic proteins (213) Presynaptic proteins (161) Prion (CD230) (24) Proteases (174) Protein ...
Wang S, Mirowski M, Sherman U, Walaszek Z, Hanausek M. Monoclonal antibodies against a 65-kDa tumor-associated phosphoprotein: ... Monoclonal antibodies against a 65-kDa tumor-associated phosphoprotein : Development and use in cancer detection. In: Hybridoma ... Monoclonal antibodies against a 65-kDa tumor-associated phosphoprotein : Development and use in cancer detection. / Wang, S.; ... Dive into the research topics of Monoclonal antibodies against a 65-kDa tumor-associated phosphoprotein: Development and use ...
Symbols: PAPP5, PP5 , PAPP5/PP5 (PROTEIN PHOSPHATASE 5); phosphoprotein phosphatase/ protein b…. ...
Both isoforms can bind to the viral phosphoprotein. The immunofluorescent staining pattern observed with ABV-infected DEFs, ...
  • Enrichment of intact phosphoproteins prior to 2-DE and LC-MS/MS appears to enhance detection of phosphorylated threonine and tyrosine residues compared with methods that utilize peptide-level enrichment, suggesting that the two approaches are somewhat complementary in terms of phosphorylation site coverage. (canada.ca)
  • Overall phosphorylation rates across significantly changed phosphoproteins were about three times higher in DFD than in normal meat. (biomedcentral.com)
  • However, fast skeletal myosin light chain 2 followed by troponin T, F-actin-capping and small heat shock proteins showed the greatest phosphorylation change, and therefore they were the most important phosphoproteins underlying LT muscle conversion to DFD meat in the Rubia Gallega breed. (biomedcentral.com)
  • The primary outcome measure was mean platelet reactivity index (PRI), as measured by vasodilator stimulated phosphoprotein (VASP) phosphorylation. (medscape.com)
  • In this light, actin-binding vasodilator-stimulated phosphoprotein (VASP) is a critical regulator of adherent cell migration, a readout of protein kinase G (PKG) activity, and a potential attractive candidate for therapeutic intervention. (ecu.edu)
  • Vasodilator-stimulated phosphoprotein regulates arterial smooth muscle cell migration (Doctoral Dissertation, East Carolina University). (ecu.edu)
  • This gene is thought to encode an extracellular matrix acidic phosphoprotein that has a function in enamel mineralization during amelogenesis. (nih.gov)
  • The psbB gene is located 562 bp upstream of the psbH gene for the 10 kDa phosphoprotein of photosystem II. (elsevier.com)
  • The gene encoding p53 phosphoprotein, originally believed to be an oncogene, recently has been proposed as a candidate antioncogene (tumor-suppressor gene). (elsevier.com)
  • 1393). Phylogenetic study based on the phosphoprotein gene of Iranian Newcastle disease viruses (NDV) isolates, 2010 -2012. (ac.ir)
  • RESULTS: Phylogenetic analysis of sequences revealed that chicken and ostrich NDV isolates were closely related and placed in the genotype VII and Pigeon Paramyxovirus-1 was located in the genotype V. CONCLUSIONS:This is the first report of Phosphoprotein gene sequences of NDV strains isolated in Iran. (ac.ir)
  • Identification of secreted phosphoprotein 1 gene as a new rheumatoid arthritis susceptibility gene. (cdc.gov)
  • A Quantitative Chemical Proteomic Strategy for Profiling Phosphoprotein Phosphatases from Yeast to Humans. (kettenbachlab.org)
  • These studies were enabled by an array of innovative technologies, including a chemical proteomicsstrategy that utilizes kinase inhibitors immobilized on beads and mass spectrometry (MS).The majority of protein dephosphorylation is carried out by phosphoprotein phosphatases (PPPs). (cancer.gov)
  • Wildtype and mutant recombinant dentin phosphoprotein (rDPP) proteins were generated using a mammalian expression system. (bvsalud.org)
  • Significantly changed phosphoproteins involved a variable number of isoforms of 13 myofibrillar and sarcoplasmic nonredundant proteins. (biomedcentral.com)
  • Affinity purification of GPI anchored urokinase type plasminogen activator receptor from chrysotile exposed cells indicated that asbestos altered the profile of proteins and phosphoprotein complexes with this receptor. (cdc.gov)
  • Chrysotile also changed the profile of proteins and phosphoproteins associated with FAK in intact cells. (cdc.gov)
  • Immunoprecipitation and subsequent gel electrophoresis of proteins from neutrophils labeled with [γ- 32 P]ATP revealed a 170 to 190-kDa phosphoprotein specifically reactive with CD15 antibodies. (umn.edu)
  • Casein (from Latin caseus, "cheese") is the name for a family of related Phosphoprotein proteins. (wordpress.com)
  • Recombinant Human Dentin Matrix Acidic Phosphoprotein 1/DMP-1 is produced by our mammalian expression system in human cells. (antikoerper-online.de)
  • Dentin Matrix Acidic Phosphoprotein 1 (DMP-1) is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. (antikoerper-online.de)
  • Two majorproteins, coded by the viral genome, are nucleocapsid protein (N) and phosphoprotein (P). The N protein protects the viralgenomic RNA and forms ribonucleoprotein complex (RNP) together with P protein. (who.int)
  • Dentin phosphoprotein inhibits lipopolysaccharide-induced macrophage activation independent of its serine/aspartic acid-rich repeats. (bvsalud.org)
  • The drug combines potent serotonin 5-HT2A receptor antagonism, dopamine receptor phosphoprotein modulation, glutamatergic modulation, and serotonin reuptake inhibition, according to Intra- Cellular Therapies. (medscape.com)
  • DARPP-32, a dopamine- and adenosine 3':5'-monophosphate-regulated phosphoprotein enriched in dopamine-innervated brain regions. (cornell.edu)
  • Five hybridoma cell lines secreting monoclonal antibodies (MAbs) to a 65- kDa tumor-associated phosphoprotein (p65) were established. (uthscsa.edu)
  • These antibodies, as well as several other widely used mAb reactive with human neutrophils, were employed to detect phosphoproteins present on these cells. (umn.edu)
  • H.J. Vogel, Phosphorus-31 Nuclear Magnetic Resonance of Phosphoproteins. (elsevier.com)
  • Competent cell lines for inducible phosphoprotein expression TKB1 Competent Cells are derived from the BL21. (thomassci.com)
  • A DNA sequence encoding the Homo sapiens (Human) Stress-induced-phosphoprotein 1, was expressed in the hosts and tags indicated. (fishersci.fi)
  • Identification of a calcium-regulated insulinoma cell phosphoprotein as an islet cell keratin. (rupress.org)
  • For this purpose, two-dimensional gel electrophoresis (2-DE), in-gel multiplex identification of phosphoproteins with PRO-Q Diamond phosphoprotein-specific stain, tandem (MALDI-TOF/TOF) mass spectrometry (MS), novel quantitative phosphoproteomic statistics and bioinformatic tools were used. (biomedcentral.com)
  • Through a meta-prediction of disorder, we localized a folded C-terminal domain in the amino acid sequence of its phosphoprotein. (rcsb.org)
  • Human Parainfluenza Virus 3 Phosphoprotein Is a Tetramer and Shares Structural and Interaction Features with Ebola Phosphoprotein VP35. (oregonstate.edu)
  • Increased circulating cell signalling phosphoproteins in sera are useful forthe detection of pancreatic cancer. (nii.ac.jp)
  • This assay has high sensitivity and excellent specificity for detection of Secreted Phosphoprotein 2 (SPP2). (biomatik.com)
  • Recently, it was shown that this phosphoprotein is one of the major substrates of ecto-protein kinase activity on human neutrophils. (umn.edu)
  • ARPP-21 is a cyclic AMP-regulated phosphoprotein of M(r) 21 kDa that is enriched in the cell bodies and terminals of medium-sized spiny neurons in the basal ganglia. (nih.gov)
  • Phosphoproteins are available in egg yolk, milk, and bone marrow. (toppr.com)
  • en] Malignant melanoma is a highly aggressive form of skin cancer responsible for the majority of skin cancer-related deaths. (uni.lu)
  • The objective of this study was to investigate the effects of dentin phosphoprotein (DPP) on lipopolysaccharide -induced inflammatory responses of macrophages in vitro . (bvsalud.org)
  • More than half of the 82 identified phosphoproteins were involved in carbohydrate metabolism, photosynthesis/respiration or oxidative stress response mechanisms. (canada.ca)
  • The roles for the 170- to 190-kDa phosphoprotein and the ecto-protein kinase in neutrophil function remain to be determined. (umn.edu)
  • Any process that stops or reduces the activity of a phosphoprotein phosphatase. (gsea-msigdb.org)
  • The Phosphoprotein Phosphatase (PPP) family members are serine/threonine phosphatases. (flybase.org)
  • Cysteamine is a Potent Inhibitor of Duodenal Phosphoprotein Phosphatase in Rats: Probable Role of Gastric Acid Hypersecretion. (routledge.com)
  • Lymphocyte phosphatase-associated phosphoprotein proteoforms analyzed using monoclonal antibodies. (hcdm.org)
  • Product Name: Human cAMP-Regulated Phosphoprotein 19 (ARP19) Antibody (APC Conjugate)Description: Purified antibody validated for. (emlinhibitor.com)
  • This study presents a general strategy to isolate and identify phosphoproteins in extracellular vesicles (EVs) from human plasma as potential markers to differentiate disease from healthy states. (pnas.org)
  • To get around this limitation, Iliuk and his colleagues looked for phosphoproteins in circulating extracellular vesicles (EVs) like microvesicles and exosomes, which are known to contain genetic material and proteins from their cells of origin. (genomeweb.com)
  • Enrichment of intact phosphoproteins prior to 2-DE and LC-MS/MS appears to enhance detection of phosphorylated threonine and tyrosine residues compared with methods that utilize peptide-level enrichment, suggesting that the two approaches are somewhat complementary in terms of phosphorylation site coverage. (canada.ca)
  • The approach, which combines a microfluidics-based capture of microvesicles and exosomes with Tymora's phosphoprotein enrichment reagents, could allow researchers to profile tumor signaling pathways via blood or urine samples, similar to how liquid biopsies make use of ctDNA analysis, said Tymora President and CTO Anton Iliuk. (genomeweb.com)
  • Iliuk cited as one key to the work a phosphoprotein enrichment method Tymora has been developing under a Phase II SBIR grant from the National Institutes of Health the company received in 2015. (genomeweb.com)
  • 13. Identification of p65-associated phosphoproteins by mass spectrometry after on-plate phosphopeptide enrichment using polymer-oxotitanium films. (nih.gov)
  • Comparative analysis of phytohormone-responsive phosphoproteins in Arabidopsis thaliana using TiO2-phosphopeptide enrichment and mass accuracy precursor alignment. (mpg.de)
  • Protein phosphorylation is a major regulatory mechanism for many cellular functions, but no phosphoprotein in biofluids has been developed for disease diagnosis because of the presence of active phosphatases. (pnas.org)
  • Protein phosphorylation, which plays a key role in most cellular activities, is a reversible process mediated by protein kinases and phosphoprotein phosphatases. (embl.de)
  • Phosphoprotein phosphatases catalyse the reverse process. (embl.de)
  • The vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena/VASP-family, regulating actin and cytoskeleton dynamics. (biomedcentral.com)
  • N. P. Brindle, M. R. Holt, J. E. Davies, C. J. Price, D. R. CritchleyThe focal-adhesion vasodilator-stimulated phosphoprotein (VASP) binds to the proline-rich domain in vinculinIn Biochem J, volume 318 ( Pt 3), 1996. (fau.de)
  • also DOC-2/Differentially-expressed in Ovarian Carcinoma and p96) is a cytoplasmic phosphoprotein. (rndsystems.com)
  • A cytoplasmic phosphoprotein involved in cytoskeletal regulation. (abcam.com)
  • The state of protein phosphorylation can be a key determinant of cellular physiology such as early-stage cancer, but the development of phosphoproteins in biofluids for disease diagnosis remains elusive. (pnas.org)
  • Background: Phosphorylation condition of dopamine- and cAMP-regulated phosphoprotein, molecular pounds 32kDa (DARPP32) is vital to comprehend drug-mediated synaptic plasticity. (hsp90-inhibitor.com)
  • Oxidative stress impairs cGMP-dependent protein kinase activation and vasodilator-stimulated phosphoprotein serine-phosphorylation. (uh.edu)
  • 2009). Using this approach we have identified many kinase client relationships, including three different protein kinases responsible for over ten phosphorylation events on a single phosphoprotein. (upsc.se)
  • CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. (thermofisher.com)
  • ARPP-21, a cAMP-regulated phosphoprotein enriched in dopamine-innervated brain regions: tissue distribution and regulation of phosphorylation in rat brain. (cornell.edu)
  • Mononegavirales encode a viral phosphoprotein (P) that binds both the viral polymerase (L) and the viral nucleoprotein (N). The P protein acts as a molecular chaperone and is required for the formation of a functional replication/transcription complex. (nih.gov)
  • Phosphoprotein (P) of negative sense RNA viruses functions as a transcriptional transactivator of the viral polymerase (L). We report here the characterization of oligomeric P protein of rinderpest virus (RPV) and provide a structural basis for its multimerization. (iisc.ac.in)
  • CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. (microarraystation.com)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Acidic Salivary Proline Rich Phosphoprotein 2 (PRH2) in tissue homogenates, cell lysates and other biological fluids. (myelisakit.com)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Acidic Salivary Proline Rich Phosphoprotein 2 (PRH2) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (myelisakit.com)
  • Phosphorylated vasodilator-stimulated phosphoprotein (P-VASPSer239) in platelets is increased by nitrite and partially deoxygenated erythrocytes. (nih.gov)
  • Platelet inhibition and increased phosphorylated vasodilator-stimulated phosphoprotein following sodium nitrite inhalation. (nih.gov)
  • Overexpression of Secreted Phosphoprotein 1 (SPP1) predicts poor survival in HPV positive cervical cancer. (cdc.gov)
  • Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. (fishersci.com)
  • This study demonstrates that the development of phosphoproteins in plasma EVs as disease biomarkers is highly feasible and may transform cancer screening and monitoring. (pnas.org)
  • 1 X 96-well Filter Plate, x-Plex assay format that includes premixed coupled beads, detection antibodies, and lysates for detecting total Phosphoprotein non-magnetic MEK1 (Ser217/Ser221), NF-kappaB p65 (Ser536), p38 MAPK (Thr180/Tyr182), requires phosphoprotein reagent and cell lysis kit offered separately. (bio-rad.com)
  • Although Dopamine and cAMP-regulated phosphoprotein, Mr 32000 (DARPP-32) is overexpressed in two-thirds of gastric cancers, its impact on molecular functions has not been fully characterized. (aacrjournals.org)
  • ARPP-21, a cyclic AMP-regulated phosphoprotein enriched in dopamine-innervated brain regions. (cornell.edu)
  • Our Orbitrap Fusion is available to support TMT based quantitative proteomics experiments, including global analysis of phosphoproteins, and we've included some example prices for TMT analysisin the cost section. (ohsu.edu)
  • The other aspect, which Iliuk and his colleagues have developed since the work presented in the PNAS study, is an improved workflow for capturing target EVs and extracting the phosphoproteins inside. (genomeweb.com)
  • The data reveal the phosphoprotein to assemble into a stable tetramer, with the regions outside the oligomerization domain remaining highly flexible. (nih.gov)
  • Tsai TL、 Wang V、 Hsiao HA、 Lu JF、 Chen HM,「Evaluation of the chromatographic parameters affecting the purification efficacy in IMAC based phosphoprotein purification: Is there a universal IMAC condition? (fju.edu.tw)
  • More than half of the 82 identified phosphoproteins were involved in carbohydrate metabolism, photosynthesis/respiration or oxidative stress response mechanisms. (canada.ca)
  • src family associated phosphoprotein 1.SKAP55.src family associated phosphoprotein 1. (stanford.edu)
  • Analysis by partial proteolysis indicated that it is related to a 23-kDa phosphoprotein present in H2ts-1 virions assembled in infected cells maintained at 39°C. Affinity labeling with [ 3 H]diisopropyl fluorophosphate showed that the 19-kDa protein contains the active site for a serine protease. (princeton.edu)
  • 8. Phosphoproteins analysis in plants: a proteomic approach. (nih.gov)
  • The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample. (perkinelmer.com)
  • In this study, the structure of the oligomerization domain of the phosphoprotein determined by X-ray crystallography is reported. (nih.gov)
  • Chatterjee, PK & Flint, SJ 1987, ' Adenovirus type 2 endopeptidase: An unusual phosphoprotein enzyme matured by autocatalysis ', Proceedings of the National Academy of Sciences of the United States of America , vol. 84, no. 3, pp. 714-718. (princeton.edu)
  • We identified close to 10,000 unique phosphopeptides in EVs from small volumes of plasma samples and more than 100 phosphoproteins in plasma EVs that are significantly higher in patients diagnosed with breast cancer as compared with healthy controls. (pnas.org)
  • Expression and clinicopathological significance of oestrogen-responsive ezrin-radixin-moesin-binding phosphoprotein 50 in breast cancer. (nih.gov)