Dopamine and cAMP-Regulated Phosphoprotein 32
A phosphoprotein that was initially identified as a major target of DOPAMINE activated ADENYLYL CYCLASE in the CORPUS STRIATUM. It regulates the activities of PROTEIN PHOSPHATASE-1 and PROTEIN KINASE A, and it is a key mediator of the biochemical, electrophysiological, transcriptional, and behavioral effects of DOPAMINE.
Phosphorylation
Phosphoprotein Phosphatases
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Amino Acid Sequence
Protein Kinases
Microfilament Proteins
Synapsins
A family of synaptic vesicle-associated proteins involved in the short-term regulation of NEUROTRANSMITTER release. Synapsin I, the predominant member of this family, links SYNAPTIC VESICLES to ACTIN FILAMENTS in the presynaptic nerve terminal. These interactions are modulated by the reversible PHOSPHORYLATION of synapsin I through various signal transduction pathways. The protein is also a substrate for cAMP- and CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASES. It is believed that these functional properties are also shared by synapsin II.
Stathmin
A ubiquitous phosphoprotein that serves as an intracellular substrate for a variety of SIGNAL TRANSDUCTION PATHWAYS. PHOSPHORYLATION of stathmin occurs during CELL CYCLE progression, and stathmin functions as a microtubule-destabilizing protein that promotes MICROTUBULE depolymerization during INTERPHASE and late MITOSIS. Stathmin is expressed at very high levels in a variety of human CANCERS.
Phosphorus Radioisotopes
Electrophoresis, Polyacrylamide Gel
Phosphothreonine
Phosvitin
Electrophoresis, Gel, Two-Dimensional
Peptide Mapping
Analysis of PEPTIDES that are generated from the digestion or fragmentation of a protein or mixture of PROTEINS, by ELECTROPHORESIS; CHROMATOGRAPHY; or MASS SPECTROMETRY. The resulting peptide fingerprints are analyzed for a variety of purposes including the identification of the proteins in a sample, GENETIC POLYMORPHISMS, patterns of gene expression, and patterns diagnostic for diseases.
Base Sequence
Cell Adhesion Molecules
Osteopontin
Sialoglycoproteins
Serine
Protein Binding
Signal Transduction
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Rabies virus
Viral Structural Proteins
Viral proteins that are components of the mature assembled VIRUS PARTICLES. They may include nucleocapsid core proteins (gag proteins), enzymes packaged within the virus particle (pol proteins), and membrane components (env proteins). These do not include the proteins encoded in the VIRAL GENOME that are produced in infected cells but which are not packaged in the mature virus particle,i.e. the so called non-structural proteins (VIRAL NONSTRUCTURAL PROTEINS).
Cloning, Molecular
Precipitin Tests
Casein Kinase II
Lyssavirus
Tyrosine
Borna disease virus
A species in the genus Bornavirus, family BORNAVIRIDAE, causing a rare and usually fatal encephalitic disease in horses and other domestic animals and possibly deer. Its name derives from the city in Saxony where the condition was first described in 1894, but the disease occurs in Europe, N. Africa, and the Near East.
Membrane Proteins
Nuclear Proteins
Nucleocapsid Proteins
Binding Sites
HeLa Cells
Sequence Homology, Amino Acid
Cyclic AMP
Cattle
Okadaic Acid
Cell Nucleus
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Recombinant Fusion Proteins
Phosphotyrosine
Zyxin
Substrate Specificity
Measles virus
Dentin
Protein Processing, Post-Translational
Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.
Proteins
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Nucleocapsid
Protein Phosphatase 1
A eukayrotic protein serine-threonine phosphatase subtype that dephosphorylates a wide variety of cellular proteins. The enzyme is comprised of a catalytic subunit and regulatory subunit. Several isoforms of the protein phosphatase catalytic subunit exist due to the presence of multiple genes and the alternative splicing of their mRNAs. A large number of proteins have been shown to act as regulatory subunits for this enzyme. Many of the regulatory subunits have additional cellular functions.
Cells, Cultured
Blotting, Western
Casein Kinases
A group of protein-serine-threonine kinases that was originally identified as being responsible for the PHOSPHORYLATION of CASEINS. They are ubiquitous enzymes that have a preference for acidic proteins. Casein kinases play a role in SIGNAL TRANSDUCTION by phosphorylating a variety of regulatory cytoplasmic and regulatory nuclear proteins.
Protein-Tyrosine Kinases
Phosphorylase Phosphatase
Threonine
Cyclic GMP-Dependent Protein Kinases
Cyclic AMP-Dependent Protein Kinases
Adaptor Proteins, Signal Transducing
A broad category of carrier proteins that play a role in SIGNAL TRANSDUCTION. They generally contain several modular domains, each of which having its own binding activity, and act by forming complexes with other intracellular-signaling molecules. Signal-transducing adaptor proteins lack enzyme activity, however their activity can be modulated by other signal-transducing enzymes
GAP-43 Protein
A nervous tissue specific protein which is highly expressed in NEURONS during development and NERVE REGENERATION. It has been implicated in neurite outgrowth, long-term potentiation, SIGNAL TRANSDUCTION, and NEUROTRANSMITTER release. (From Neurotoxicology 1994;15(1):41-7) It is also a substrate of PROTEIN KINASE C.
Protein-Serine-Threonine Kinases
Rabbits
Vesiculovirus
Carrier Proteins
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Transfection
Enzyme Activation
Phosphoric Monoester Hydrolases
Protein Phosphatase 2
A phosphoprotein phosphatase subtype that is comprised of a catalytic subunit and two different regulatory subunits. At least two genes encode isoforms of the protein phosphatase catalytic subunit, while several isoforms of regulatory subunits exist due to the presence of multiple genes and the alternative splicing of their mRNAs. Protein phosphatase 2 acts on a broad variety of cellular proteins and may play a role as a regulator of intracellular signaling processes.
Protein Kinase C
An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.
Amino Acids
Vesicular stomatitis Indiana virus
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Cytoskeletal Proteins
Subcellular Fractions
Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)
Viral Matrix Proteins
Mutation
DNA, Complementary
Calcium
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
GTP-Binding Protein Regulators
Proteins that regulate the signaling activity of GTP-BINDING PROTEINS. They are divided into three categories depending upon whether they stimulate GTPase activity (GTPASE-ACTIVATING PROTEINS), inhibit release of GDP; (GUANINE NUCLEOTIDE DISSOCIATION INHIBITORS); or exchange GTP for GDP; (GUANINE NUCLEOTIDE EXCHANGE FACTORS).
Cell Nucleolus
Within most types of eukaryotic CELL NUCLEUS, a distinct region, not delimited by a membrane, in which some species of rRNA (RNA, RIBOSOMAL) are synthesized and assembled into ribonucleoprotein subunits of ribosomes. In the nucleolus rRNA is transcribed from a nucleolar organizer, i.e., a group of tandemly repeated chromosomal genes which encode rRNA and which are transcribed by RNA polymerase I. (Singleton & Sainsbury, Dictionary of Microbiology & Molecular Biology, 2d ed)
Henipavirus
Chromatography, Affinity
Paramyxoviridae
Intracellular Signaling Peptides and Proteins
Proteins and peptides that are involved in SIGNAL TRANSDUCTION within the cell. Included here are peptides and proteins that regulate the activity of TRANSCRIPTION FACTORS and cellular processes in response to signals from CELL SURFACE RECEPTORS. Intracellular signaling peptide and proteins may be part of an enzymatic signaling cascade or act through binding to and modifying the action of other signaling factors.
Cytomegalovirus
A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, infecting the salivary glands, liver, spleen, lungs, eyes, and other organs, in which they produce characteristically enlarged cells with intranuclear inclusions. Infection with Cytomegalovirus is also seen as an opportunistic infection in AIDS.
Cercopithecus aethiops
Mass Spectrometry
Transcription, Genetic
Tumor Cells, Cultured
Rhabdoviridae
Macromolecular Substances
Peptide Fragments
Ribosomal Proteins
Actins
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
Manganese
A trace element with atomic symbol Mn, atomic number 25, and atomic weight 54.94. It is concentrated in cell mitochondria, mostly in the pituitary gland, liver, pancreas, kidney, and bone, influences the synthesis of mucopolysaccharides, stimulates hepatic synthesis of cholesterol and fatty acids, and is a cofactor in many enzymes, including arginase and alkaline phosphatase in the liver. (From AMA Drug Evaluations Annual 1992, p2035)
Isoelectric Point
Rats, Inbred Strains
Viral Nonstructural Proteins
Cyclic GMP
Guanosine cyclic 3',5'-(hydrogen phosphate). A guanine nucleotide containing one phosphate group which is esterified to the sugar moiety in both the 3'- and 5'-positions. It is a cellular regulatory agent and has been described as a second messenger. Its levels increase in response to a variety of hormones, including acetylcholine, insulin, and oxytocin and it has been found to activate specific protein kinases. (From Merck Index, 11th ed)
Cytosol
Magnesium
Natal Teeth
Predeciduous teeth present at birth. They may be well formed and normal or may represent hornified epithelial structures without roots. They are found on the gingivae over the crest of the ridge and arise from accessory buds of the dental lamina ahead of the deciduous buds or from buds of the accessory dental lamina. (From Jablonski, Dictionary of Dentistry, 1992)
Brain
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
Mandibulofacial Dysostosis
A hereditary disorder occurring in two forms: the complete form (Franceschetti's syndrome) is characterized by antimongoloid slant of the palpebral fissures, coloboma of the lower lid, micrognathia and hypoplasia of the zygomatic arches, and microtia. It is transmitted as an autosomal trait. The incomplete form (Treacher Collins syndrome) is characterized by the same anomalies in less pronounced degree. It occurs sporadically, but an autosomal dominant mode of transmission is suspected. (Dorland, 27th ed)
Immunoblotting
COS Cells
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
Autoradiography
Adenosine Triphosphate
Peptides
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Cell Membrane
Cytoplasm
Cricetinae
Viral Core Proteins
Paramecium
DNA-Binding Proteins
Rinderpest virus
Calmodulin-Binding Proteins
Vero Cells
Protein Biosynthesis
Mitosis
Two-Hybrid System Techniques
Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
Sequence Alignment
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Oxazoles
Immunologic Techniques
Chromatography, Ion Exchange
Caudate Nucleus
Caseins
DNA Primers
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.
Electrophoresis, Paper
Mutagenesis, Site-Directed
DNA
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Amino Acid Motifs
Trypsin
Profilins
Chromatography, DEAE-Cellulose
Tooth Calcification
Proto-Oncogene Proteins c-fyn
Neoplasm Proteins
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
Sodium-Hydrogen Antiporter
Cell Transformation, Viral
Oncogene Protein pp60(v-src)
A tyrosine-specific protein kinase encoded by the v-src oncogene of ROUS SARCOMA VIRUS. The transforming activity of pp60(v-src) depends on both the lack of a critical carboxy-terminal tyrosine phosphorylation site at position 527, and the attachment of pp60(v-src) to the plasma membrane which is accomplished by myristylation of its N-terminal glycine.
Transcription Factors
Morbillivirus
Enzyme Inhibitors
src Homology Domains
Regions of AMINO ACID SEQUENCE similarity in the SRC-FAMILY TYROSINE KINASES that fold into specific functional tertiary structures. The SH1 domain is a CATALYTIC DOMAIN. SH2 and SH3 domains are protein interaction domains. SH2 usually binds PHOSPHOTYROSINE-containing proteins and SH3 interacts with CYTOSKELETAL PROTEINS.
Cytoskeleton
Incisor
Immunosorbent Techniques
Tetradecanoylphorbol Acetate
Virus Replication
Gene Expression
Brain Chemistry
Virion
Proline-Rich Protein Domains
Protein domains that are enriched in PROLINE. The cyclical nature of proline causes the peptide bonds it forms to have a limited degree of conformational mobility. Therefore the presence of multiple prolines in close proximity to each other can convey a distinct conformational arrangement to a peptide chain.
Gene Expression Regulation
Phosphoglucomutase
Blood Platelets
Liver
Fibroblasts
RNA-Binding Proteins
Chromatography, Gel
Integrin-Binding Sialoprotein
Chickens
Calcium-Binding Proteins
Extracellular Matrix Proteins
Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).
Protein Array Analysis
src-Family Kinases
A PROTEIN-TYROSINE KINASE family that was originally identified by homology to the Rous sarcoma virus ONCOGENE PROTEIN PP60(V-SRC). They interact with a variety of cell-surface receptors and participate in intracellular signal transduction pathways. Oncogenic forms of src-family kinases can occur through altered regulation or expression of the endogenous protein and by virally encoded src (v-src) genes.
Plasmids
Sequence Homology, Nucleic Acid
Calmodulin
A heat-stable, low-molecular-weight activator protein found mainly in the brain and heart. The binding of calcium ions to this protein allows this protein to bind to cyclic nucleotide phosphodiesterases and to adenyl cyclase with subsequent activation. Thereby this protein modulates cyclic AMP and cyclic GMP levels.
Tandem Mass Spectrometry
A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.
Tissue Distribution
Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.
Proto-Oncogene Proteins
Open Reading Frames
Endocytosis: EH domains lend a hand. (1/18461)
A number of proteins that have been implicated in endocytosis feature a conserved protein-interaction module known as an EH domain. The three-dimensional structure of an EH domain has recently been solved, and is likely to presage significant advances in understanding molecular mechanisms of endocytosis. (+info)The hematopoietic-specific adaptor protein gads functions in T-cell signaling via interactions with the SLP-76 and LAT adaptors. (2/18461)
BACKGROUND: The adaptor protein Gads is a Grb2-related protein originally identified on the basis of its interaction with the tyrosine-phosphorylated form of the docking protein Shc. Gads protein expression is restricted to hematopoietic tissues and cell lines. Gads contains a Src homology 2 (SH2) domain, which has previously been shown to have a similar binding specificity to that of Grb2. Gads also possesses two SH3 domains, but these have a distinct binding specificity to those of Grb2, as Gads does not bind to known Grb2 SH3 domain targets. Here, we investigated whether Gads is involved in T-cell signaling. RESULTS: We found that Gads is highly expressed in T cells and that the SLP-76 adaptor protein is a major Gads-associated protein in vivo. The constitutive interaction between Gads and SLP-76 was mediated by the carboxy-terminal SH3 domain of Gads and a 20 amino-acid proline-rich region in SLP-76. Gads also coimmunoprecipitated the tyrosine-phosphorylated form of the linker for activated T cells (LAT) adaptor protein following cross-linking of the T-cell receptor; this interaction was mediated by the Gads SH2 domain. Overexpression of Gads and SLP-76 resulted in a synergistic augmentation of T-cell signaling, as measured by activation of nuclear factor of activated T cells (NFAT), and this cooperation required a functional Gads SH2 domain. CONCLUSIONS: These results demonstrate that Gads plays an important role in T-cell signaling via its association with SLP-76 and LAT. Gads may promote cross-talk between the LAT and SLP-76 signaling complexes, thereby coupling membrane-proximal events to downstream signaling pathways. (+info)The splicing factor-associated protein, p32, regulates RNA splicing by inhibiting ASF/SF2 RNA binding and phosphorylation. (3/18461)
The cellular protein p32 was isolated originally as a protein tightly associated with the essential splicing factor ASF/SF2 during its purification from HeLa cells. ASF/SF2 is a member of the SR family of splicing factors, which stimulate constitutive splicing and regulate alternative RNA splicing in a positive or negative fashion, depending on where on the pre-mRNA they bind. Here we present evidence that p32 interacts with ASF/SF2 and SRp30c, another member of the SR protein family. We further show that p32 inhibits ASF/SF2 function as both a splicing enhancer and splicing repressor protein by preventing stable ASF/SF2 interaction with RNA, but p32 does not block SRp30c function. ASF/SF2 is highly phosphorylated in vivo, a modification required for stable RNA binding and protein-protein interaction during spliceosome formation, and this phosphorylation, either through HeLa nuclear extracts or through specific SR protein kinases, is inhibited by p32. Our results suggest that p32 functions as an ASF/SF2 inhibitory factor, regulating ASF/SF2 RNA binding and phosphorylation. These findings place p32 into a new group of proteins that control RNA splicing by sequestering an essential RNA splicing factor into an inhibitory complex. (+info)The Gab1 PH domain is required for localization of Gab1 at sites of cell-cell contact and epithelial morphogenesis downstream from the met receptor tyrosine kinase. (4/18461)
Stimulation of the hepatocyte growth factor (HGF) receptor tyrosine kinase, Met, induces mitogenesis, motility, invasion, and branching tubulogenesis of epithelial and endothelial cell lines in culture. We have previously shown that Gab1 is the major phosphorylated protein following stimulation of the Met receptor in epithelial cells that undergo a morphogenic program in response to HGF. Gab1 is a member of the family of IRS-1-like multisubstrate docking proteins and, like IRS-1, contains an amino-terminal pleckstrin homology domain, in addition to multiple tyrosine residues that are potential binding sites for proteins that contain SH2 or PTB domains. Following stimulation of epithelial cells with HGF, Gab1 associates with phosphatidylinositol 3-kinase and the tyrosine phosphatase SHP2. Met receptor mutants that are impaired in their association with Gab1 fail to induce branching tubulogenesis. Overexpression of Gab1 rescues the Met-dependent tubulogenic response in these cell lines. The ability of Gab1 to promote tubulogenesis is dependent on its pleckstrin homology domain. Whereas the wild-type Gab1 protein is localized to areas of cell-cell contact, a Gab1 protein lacking the pleckstrin homology domain is localized predominantly in the cytoplasm. Localization of Gab1 to areas of cell-cell contact is inhibited by LY294002, demonstrating that phosphatidylinositol 3-kinase activity is required. These data show that Gab1 is an important mediator of branching tubulogenesis downstream from the Met receptor and identify phosphatidylinositol 3-kinase and the Gab1 pleckstrin homology domain as crucial for subcellular localization of Gab1 and biological responses. (+info)The histone acetylase PCAF is a phorbol-ester-inducible coactivator of the IRF family that confers enhanced interferon responsiveness. (5/18461)
Transcription factors of the interferon regulatory factor (IRF) family bind to the type I interferon (IFN)-responsive element (ISRE) and activate transcription from IFN-inducible genes. To identify cofactors that associate with IRF proteins, DNA affinity binding assays were performed with nuclear extracts prepared from tissue culture cells. The results demonstrated that the endogenous IRFs bound to the ISRE are complexed with the histone acetylases, PCAF, GCN5, and p300/CREB binding protein and that histone acetylase activities are accumulated on the IRF-ISRE complexes. By testing recombinant proteins, we show that PCAF directly binds to some but not all members of the IRF family through distinct domains of the two proteins. This interaction was functionally significant, since transfection of PCAF strongly enhanced IRF-1- and IRF-2-dependent promoter activities. Further studies showed that expression of PCAF and other histone acetylases was markedly induced in U937 cells upon phorbol ester treatment, which led to increased recruitment of PCAF to the IRF-ISRE complexes. Coinciding with the induction of histone acetylases, phorbol ester markedly enhanced IFN-alpha-stimulated gene expression in U937 cells. Supporting the role for PCAF in conferring IFN responsiveness, transfection of PCAF into U937 cells led to a large increase in IFN-alpha-inducible promoter activity. These results demonstrate that PCAF is a phorbol ester-inducible coactivator of the IRF proteins which contributes to the establishment of type I IFN responsiveness. (+info)Mutations of oncoprotein 18/stathmin identify tubulin-directed regulatory activities distinct from tubulin association. (6/18461)
Oncoprotein 18/stathmin (Op18) is a recently identified phosphorylation-responsive regulator of the microtubule (MT) system. It was originally proposed that Op18 specifically regulates dynamic properties of MTs by associating with tubulin, but it has subsequently been proposed that Op18 acts simply by sequestering of tubulin heterodimers. We have dissected the mechanistic action of Op18 by generation of two distinct classes of mutants. One class has interruptions of the heptad repeats of a potential coiled-coil region of Op18, and the other involves substitution at all four phosphorylation sites with negatively charged Glu residues. Both types of mutation result in Op18 proteins with a limited decrease in tubulin complex formation. However, the MT-destabilizing activities of the coiled-coil mutants are more severely reduced in transfected leukemia cells than those of the Glu-substituted Op18 derivative, providing evidence for tubulin-directed regulatory activities distinct from tubulin complex formation. Analysis of Op18-mediated regulation of tubulin GTPase activity and taxol-promoted tubulin polymerization showed that while wild-type and Glu-substituted Op18 derivatives are active, the coiled-coil mutants are essentially inactive. This suggests that Op18-tubulin contact involves structural motifs that deliver a signal of regulatory importance to the MT system. (+info)BLNK required for coupling Syk to PLC gamma 2 and Rac1-JNK in B cells. (7/18461)
Signaling through the B cell receptor (BCR) is essential for B cell function and development. Despite the key role of Syk in BCR signaling, little is known about the mechanism by which Syk transmits downstream effectors. BLNK (B cell LiNKer protein), a substrate for Syk, is now shown to be essential in activating phospholipase C (PLC)gamma 2 and JNK. The BCR-induced PLC gamma 2 activation, but not the JNK activation, was restored by introduction of PLC gamma 2 membrane-associated form into BLNK-deficient B cells. As JNK activation requires both Rac1 and PLC gamma 2, our results suggest that BLNK regulates the Rac1-JNK pathway, in addition to modulating PLC gamma 2 localization. (+info)Characterization and partial purification of a novel neutrophil membrane-associated kinase capable of phosphorylating the respiratory burst component p47phox. (8/18461)
The phosphorylation of p47phox is widely viewed as an important step in the activation of the neutrophil respiratory burst oxidase system. The exact nature of the kinase(s) responsible remains to be elucidated. We show here that such a kinase was detected on neutrophil membranes activated by either PMA or formyl-methionyl-leucyl-phenylalanine. This enzyme is not intrinsic to the neutrophil membrane and could be eluted with 0.5 M NaCl. The kinase activity was partially purified and was found not to be due to the presence of previously suggested kinases, including protein kinase C isotypes, mitogen-activated protein kinase and protein kinase B. Gel filtration and renaturation in substrate gels suggest a molecular mass of between 45 and 51 kDa. The kinase activity was independent of calcium and lipids but was potently inhibited by staurosporine. Treatment with protein phosphatase 2Ac suggested that the kinase was activated by serine/threonine phosphorylation. Phosphopeptide maps indicated that the kinase phosphorylated p47phox on similar sites to those found in vivo. These results indicate that activation of neutrophils by PMA results in the activation of a membrane-associated kinase that may play a part in the regulation of neutrophil NADPH oxidase through its ability to phosphorylate p47phox. (+info)
Effects of miR-34a on cellular phosphoprotein activatio | Open-i
Localization of the 47 kDa phosphoprotein involved in the respiratory-burst NADPH oxidase of phagocytic cells | Biochemical...
Cloning of a novel phosphoprotein regulated by colony-stimulating factor 1 shares a domain with the Drosophila disabled gene...
Phosphoprotein and phosphopeptide purification oveview: gravity columns, spin columns, magnetic beads
Arabidopsis FUSCA5 Encodes a Novel Phosphoprotein That Is a Component of the COP9 Complex | Plant Cell
Single-cell phospho-protein signaling analysis of the response to influenza vacci - Garry Nolan
ANP32E (acidic (leucine-rich) nuclear phosphoprotein 32 family, member E) - KOMP (Knockout Mouse Project)
ANP32A Pre-design Chimera RNAi - (H00008125-R01) - Products - Abnova
Requirement for the Leukocyte-Specific Adapter Protein SLP-76 for Normal T Cell Development | Science
Steroidogenic acute regulatory protein elisa and antibody
STAT Protein Interference and Suppression of Cytokine Signal Transduction by Measles Virus V Protein | Journal of Virology
Quantitative phosphoproteome profiling of Wnt3a-mediated signaling network: indicating the involvement of ribonucleoside...
ARPP21 overexpression lysate - LY422573 | acris-antibodies.com
Procaspase-8, the zymogen type of the apoptosis-initiator caspase-8, undergoes phosphorylation following | High-Throughput...
Exbio antibodies - Rabbit Polyclonal to SLP76
Human-gpDB
Browsing by Author Jabeen, A
Charging it up: global analysis of protein phosphorylation
TP53 antibody | pab74392 | Covalab - Covalab Biotechnology
The beta2-adrenergic receptor interacts with the Na+/H+-exchanger regulatory factor to control Na+/H+ exchange
OriGene - VASP (NM 003370) cDNA Clone
PALM paralemmin [Homo sapiens (human)] - Gene - NCBI
anti-VASP (phospho Ser239) antibody | GeneTex
Protein phosphorylation | Abcam
Vasodilator Stimulated Phosphoprotein | DR Tools
IMP: Integrative Multi-species Prediction
Overview of Protein Phosphorylation - Current Protocols
Phosphoflow
Fertilizer - Birchs Lawn Care & Landscaping Kennewick, Pasco & Richland
Endosomal localization and receptor dynamics determine tyrosine phosphorylation of hepatocyte growth factor-regulated tyrosine...
Endocytic pathways regulate Toll-like receptor 4 signaling and link innate and adaptive immunity. - Semantic Scholar
RCSB PDB - Protein Feature View
- Hepatocyte growth factor-regulated tyrosine kinase substrate - Q960X8 (HRS DROME)
cDNA Cloning and Overexpression of Acidic Ribosomal Phosphoprotein P1 Gene (RPLP1) from the Giant Panda [Abstract]
Expression and function of the insulin receptor substrate proteins in by Katerina Mardilovich, Shannon L. Pankratz et al.
VackvSuG: Human erythroid p55, a palmitoylated peripheral membrane phosphoprotein
Cloning, Expression and Hormonal Regulation of Steroidogenic Acute Regulatory Protein Gene in Buffalo Ovary | Korea Science
Dentin matrix acidic phosphoprotein 1 (pig) | Protein Target - PubChem
中国科学院大连化学物理研究所机构知识库(DICP OpenIR): Systematic Analysis of Protein Phosphorylation Networks From Phosphoproteomic Data
Characterization of the Borna disease virus phosphoprotein, p2...
A 48 kDa integral membrane phosphoprotein orchestrates the cytoskeletal dynamics that generate amoeboid cell motility in...
Synaptic vesicle phosphoproteins and regulation of synaptic function | Science
Study of Radiation (RT) Concurrent With Cetuximab in Patients With Advanced Head and Neck Squamous Cell Carcinoma (SCC) - Full...
Two-dimensional gel electrophoresis of nuclear phosphoproteins...
Combined integrin phosphoproteomic analyses and small interfering RNA-based functional screening identify key regulators for...
Fyn-Binding Protein (Fyb)/Slp-76-Associated Protein (Slap), Ena/Vasodilator-Stimulated Phosphoprotein (Vasp) Proteins and the...
Mutagenetix > Incidental...
YAP1 (yes-associated protein 1) - KOMP (Knockout Mouse Project)
Ser1296
Thr702
Disrupting the Oncogenic Synergis... preview & related info | Mendeley
M-phase phosphoprotein 6 (IPR019324) | InterPro | EMBL-EBI
Phosphoprotein (Q8B6J8) | InterPro | EMBL-EBI
c-Myc - TA500002 | acris-antibodies.com
BRCA-1 Antibody - Biocare Medical
JoVE Author Search: Brewis IA
小鼠Paxillin (phospho S83)肽(ab42724)|Abcam中国
Phosphoprotein
Phosphoproteins have been proposed as biomarkers for breast cancer. Protein phosphorylation Keyword - Phosphoprotein ... Liquid Biopsy Could Get Boost from Phosphoproteins. 2017 v t e (Phosphoproteins, All stub articles, Protein stubs). ... A phosphoprotein is a protein that is posttranslationally modified by the attachment of either a single phosphate group, or a ... Phosphoproteins in extracellular vesicles as candidate markers for breast cancer Cozzone AJ (1988). "Protein phosphorylation in ...
Dentin phosphoprotein
... , or phosphophoryn, is one of three proteins formed from dentin sialophosphoprotein and is important in ... Being highly negative, dentin phosphoprotein is able to attract large amounts of calcium. In vitro studies also indicate ...
Vasodilator-stimulated phosphoprotein
... (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an N- ... Vasodilator-stimulated phosphoprotein has been shown to interact with Zyxin, Profilin 1, and PFN2. GRCh38: Ensembl release 89: ... Vasodilator-stimulated phosphoprotein is a protein that in humans is encoded by the VASP gene. ... 1992). "The 46/50 kDa phosphoprotein VASP purified from human platelets is a novel protein associated with actin filaments and ...
Nucleolar phosphoprotein p130
... is a protein that in humans is encoded by the NOLC1 gene. Nucleolar phosphoprotein p130 has been ... "Entrez Gene: NOLC1 nucleolar and coiled-body phosphoprotein 1". Isaac, C; Yang Y; Meier U T (Jul 1998). "Nopp140 functions as a ... Chen HK, Yeh NH (1997). "The nucleolar phosphoprotein P130 is a GTPase/ATPase with intrinsic property to form large complexes ... Miau LH, Chang CJ, Tsai WH, Lee SC (1997). "Identification and characterization of a nucleolar phosphoprotein, Nopp140, as a ...
Translocated actin-recruiting phosphoprotein
The translocated actin-recruiting phosphoprotein (Tarp) is a protein that may mediate the invasion of epithelial cells by ... "Translocated actin-recruiting phosphoprotein [Chlamydia trachomatis] - Protein - NCBI". v t e (Protein pages needing a picture ...
Acidic leucine-rich nuclear phosphoprotein 32 family member A
... is a protein that in humans is encoded by the ANP32A gene. It is ... Acidic leucine-rich nuclear phosphoprotein 32 family member A has been shown to interact with MAP1B, TAF1A and Protein SET. ... "Entrez Gene: ANP32A Acidic (leucine-rich) nuclear phosphoprotein 32 family, member A". Opal, Puneet; Garcia Jesus J; Propst ...
3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring) kinase
Phosphoprotein. Res. Advances in Second Messenger and Phosphoprotein Research. 31: 105-11. doi:10.1016/S1040-7952(97)80012-2. ...
Kevin Lustig
"Systematic Identification of Mitotic Phosphoproteins". Current Biology. 7 (5): 338-48. doi:10.1016/s0960-9822(06)00157-6. PMID ...
Mokola lyssavirus
The genome encodes five proteins: matrix protein M; transmembrane glycoprotein G; nucleoprotein N; phosphoprotein P; and the ...
MYLK4
This protein acts as an enzyme that catalyzes the following reaction: ATP + a protein -> ADP + a phosphoprotein. MYLK4 is also ...
Tetrasodium tris(bathophenanthroline disulfonate)ruthenium(II)
Gerber, Isak B.; Laukens, Kris; Witters, Erwin; Dubery, Ian A. (2006). "Lipopolysaccharide-responsive phosphoproteins in ... "Quantitative detection of phosphoproteins by combination of two-dimensional difference gel electrophoresis and phosphospecific ...
Tandem mass spectrometry
Gafken PR, Lampe PD (2006). "Methodologies for characterizing phosphoproteins by mass spectrometry". Cell Communication & ...
Isobaric tag for relative and absolute quantitation
Gafken PR, Lampe PD (2006). "Methodologies for characterizing phosphoproteins by mass spectrometry". Cell Commun. Adhes. 13 (5- ...
Vitellin
It is a phosphoprotein. Vitellin is a generic name for major of many yolk proteins. Vitellin has been known since the 1930s. ... This phosphoprotein acts as a membrane, 1-3.5μm, that encloses the egg that comprises at least five glycoproteins that resemble ... https://doi.org/10.1016/0304-4165(86)90267-9 v t e (Use dmy dates from August 2018, Avian proteins, Phosphoproteins, All stub ...
DDX46
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. Bibcode: ...
SFRS6
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ...
NOL1
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. Bibcode: ...
SF3B1
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ...
RBMX
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ...
NFIA
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ...
RBM7
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ...
BAZ1B
August 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of ...
OSBPL11
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ...
Cyclin T2
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. Bibcode: ...
PALB2
August 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of ...
STRN3
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. Bibcode: ...
MINK1
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ...
SENP6
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ...
LGTN
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ...
LARP1
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ...
ODAPH odontogenesis associated phosphoprotein [Homo sapiens (human)] - Gene - NCBI
odontogenesis associated phosphoproteinprovided by HGNC. Primary source. HGNC:HGNC:26300 See related. Ensembl:ENSG00000174792 ... This gene is thought to encode an extracellular matrix acidic phosphoprotein that has a function in enamel mineralization ... NM_001206981.2 → NP_001193910.1 odontogenesis associated phosphoprotein isoform 1 precursor. See identical proteins and their ... NM_001257072.2 → NP_001244001.1 odontogenesis associated phosphoprotein isoform 3 precursor. See identical proteins and their ...
RCSB PDB - 3T4R: Lettuce Necrotic Yellow Virus Phosphoprotein C-Terminal Domain
Lettuce Necrotic Yellow Virus Phosphoprotein C-Terminal Domain ... Lettuce Necrotic Yellow Virus Phosphoprotein C-Terminal Domain ... Phosphoprotein. A. 81. Lettuce necrotic yellows virus isolate 318. Mutation(s): 0 Gene Names: P. ... Structure of the C-terminal domain of lettuce necrotic yellows virus phosphoprotein.. Martinez, N., Ribeiro, E.A., Leyrat, C., ... we localized a folded C-terminal domain in the amino acid sequence of its phosphoprotein. This domain consists of an autonomous ...
Drugs of abuse modulate the phosphorylation of ARPP-21, a cyclic AMP-regulated phosphoprotein enriched in the basal ganglia
ARPP-21 is a cyclic AMP-regulated phosphoprotein of M(r) 21 kDa that is enriched in the cell bodies and terminals of medium- ... ARPP-21 is a cyclic AMP-regulated phosphoprotein of M(r) 21 kDa that is enriched in the cell bodies and terminals of medium- ... Drugs of abuse modulate the phosphorylation of ARPP-21, a cyclic AMP-regulated phosphoprotein enriched in the basal ganglia ...
Anp32e MGI Mouse Gene Detail - MGI:1913721 - acidic (leucine-rich) nuclear phosphoprotein 32 family, member E
Human Dentin Matrix Acidic phosphoprotein 1 (DMP1) Protein (His tag), Recombinant | ABIN1691401
Recombinant Dentin Matrix Acidic phosphoprotein 1 (DMP1) Protein (His tag). Spezies: Human. Quelle: Human Cells. Jetzt Produkt ... DMP1 (Dentin Matrix Acidic phosphoprotein 1 (DMP1)) Andere Bezeichnung Dentin Matrix Acidic Phosphoprotein 1 (DMP1 Produkte) ... Dentin Matrix Acidic phosphoprotein 1 (DMP1) (AA 17-489) protein (His tag) DMP1 Spezies: Ratte Wirt: Hefe Recombinant > 90 % ... Dentin Matrix Acidic phosphoprotein 1 (DMP1) (AA 17-510) protein (His tag) DMP1 Spezies: Rind (Kuh) Wirt: Hefe Recombinant > 90 ...
LS Protein Expression Solutions Competent Cells for Routine Protein Expression Phosphoprotein Generation
Enhance phospho-protein identification and recovery for Western blot | Tebubio's blog
Want to preserve phospho-proteins for Western blot and Antibody array protocols?. Phospho-Sure™ RTD™ Neuronal Cell & Soft ... Phospho-proteins can be easily monitored by using phospho-specific antibodies in various types of immuno-assays (Western blot, ... 3 tips to preserve phospho-proteins with home-made lysis buffers. As soon as the lysis buffer is added on samples, these are ... 1 alternative: ready-to-dilute non-toxic lysis buffer optimized for phospho-protein isolation. Comparison of the signal ...
Differential phosphoprotein labeling (DIPPL), a method for comparing live cell phosphoproteomes using simultaneous analysis of...
Wyttenbach, A. and Tolkovsky, A.M. (2006) Differential phosphoprotein labeling (DIPPL), a method for comparing live cell ... Differential phosphoprotein labeling (DIPPL), a method for comparing live cell phosphoproteomes using simultaneous analysis of ... Differential phosphoprotein labeling (DIPPL), a method for comparing live cell phosphoproteomes using simultaneous analysis of ... Differential phosphoprotein labeling (DIPPL), a method for comparing live cell phosphoproteomes using simultaneous analysis of ...
Expression of the nucleoprotein and phosphoprotein genes of pneumonia virus of mice and specific interactions of the gene...
gene and the phosphoprotein. (P). gene of. PVM. and attempted to. characterise the polypeptide products expressed from the N. ... Barr, John Nicholas (1993) Expression of the nucleoprotein and phosphoprotein genes of pneumonia virus of mice and specific ... Expression of the nucleoprotein and phosphoprotein genes of pneumonia virus of mice and specific interactions of the gene ... Nucleoproteins , Phosphoproteins, Viral genetics, Viral pneumonia , Pneumonia, Virus diseases -- Research, Genetics -- Research ...
Differential expression of the psbB and psbH genes encoding the 47 kDa chlorophyll a-protein and the 10 kDa phosphoprotein of...
The psbB gene is located 562 bp upstream of the psbH gene for the 10 kDa phosphoprotein of photosystem II. A small open reading ... The psbB gene is located 562 bp upstream of the psbH gene for the 10 kDa phosphoprotein of photosystem II. A small open reading ... The psbB gene is located 562 bp upstream of the psbH gene for the 10 kDa phosphoprotein of photosystem II. A small open reading ... The psbB gene is located 562 bp upstream of the psbH gene for the 10 kDa phosphoprotein of photosystem II. A small open reading ...
Identification of a calcium-regulated insulinoma cell phosphoprotein as an islet cell keratin. | Journal of Cell Biology |...
Increased circulating cell signalling phosphoproteins in sera are useful forthe detection of pancreatic cancer. | CiNii Research
JCI Insight -
Mutations of MAP1B encoding a microtubule-associated phosphoprotein cause sensorineural hearing loss
Advances in second messenger and phosphoprotein research
Human Secreted Phosphoprotein 2 (SPP2) ELISA Kit, Cat#EKU07225 - Biomatik
Human Secreted Phosphoprotein 2 (SPP2) ELISA Kit, Cat#EKU07225. Write a Review Write a Review. × ... Human Secreted Phosphoprotein 2 (SPP2) ELISA Kit, Cat#EKU07225. Rating Required Select Rating. 1 star (worst). 2 stars. 3 stars ... This assay has high sensitivity and excellent specificity for detection of Secreted Phosphoprotein 2 (SPP2). No significant ... cross-reactivity or interference between Secreted Phosphoprotein 2 (SPP2) and analogues was observed. ...
Immunohistochemical evidence of abnormal expression of the antioncogene-encoded p53 phosphoprotein in Hodgkin's disease and...
N2 - The gene encoding p53 phosphoprotein, originally believed to be an oncogene, recently has been proposed as a candidate ... AB - The gene encoding p53 phosphoprotein, originally believed to be an oncogene, recently has been proposed as a candidate ... The gene encoding p53 phosphoprotein, originally believed to be an oncogene, recently has been proposed as a candidate ... abstract = "The gene encoding p53 phosphoprotein, originally believed to be an oncogene, recently has been proposed as a ...
SP110 gene: MedlinePlus Genetics
Projects | Center for Cancer Nanotechnology Excellence for Translational Diagnostics | Stanford Medicine
Top 100 in Cell and Molecular Biology
Src Kinase Associated Phosphoprotein 1 (SKAP1) Protein | Abbexa Ltd
SCAP1, SKAP55, pp55, Src Family Associated Phosphoprotein 1, Src kinase-associated phosphoprotein of 55 kDa, SCAP1 protein, ... Src kinase-associated phosphoprotein of 55 kDa protein, Protein Src kinase-associated phosphoprotein of 55 kDa, Rat Src kinase- ... associated phosphoprotein of 55 kDa Protein, Rat Src kinase-associated phosphoprotein of 55 kDa, Rat protein Src kinase- ... Src Family Associated Phosphoprotein 1 protein, Protein Src Family Associated Phosphoprotein 1, Rat Src Family Associated ...
Vasodilator-stimulated phosphoprotein regulates arterial smooth muscle cell migration
In this light, actin-binding vasodilator-stimulated phosphoprotein (VASP) is a critical regulator of adherent cell migration, a ... Holt, Andrew W. Vasodilator-stimulated phosphoprotein regulates arterial smooth muscle cell migration. Doctoral Dissertation. ... Holt, Andrew W. (September 2016). Vasodilator-stimulated phosphoprotein regulates arterial smooth muscle cell migration ( ... Holt, Andrew W. Vasodilator-stimulated phosphoprotein regulates arterial smooth muscle cell migration [Doctoral Dissertation]. ...
Table - Proposal for Human Respiratory Syncytial Virus Nomenclature below the Species Level - Volume 27, Number 6-June 2021 -...
IMSEAR at SEARO: Measles virus phosphoprotein inhibits apoptosis and enhances clonogenic and migratory properties in HeLa cells
Measles virus phosphoprotein inhibits apoptosis and enhances clonogenic and migratory properties in HeLa cells. Journal of ... Measles virus phosphoprotein inhibits apoptosis and enhances clonogenic and migratory properties in HeLa cells. ... Two majorproteins, coded by the viral genome, are nucleocapsid protein (N) and phosphoprotein (P). The N protein protects the ...
Phlpp2 PH domain and leucine rich repeat protein phosphatase 2 [Mus musculus (house mouse)] - Gene - NCBI
Mouse Src kinase-associated phosphoprotein 2 (Skap2) GENTAUR-58bd6a0aa4e2d | Gentaursearch
Mouse Src kinase-associated phosphoprotein 2 (Skap2) datasheet and description hight quality product and Backed by our ... src family associated phosphoprotein 2, src family-associated phosphoprotein 2, src kinase-associated phosphoprotein 2, src ... Src kinase-associated phosphoprotein 2, Src kinase-associated phosphoprotein 55-related protein, Src-associated adapter protein ... Recombinant Mouse Src kinase-associated phosphoprotein 2 (Skap2). Alternative names: Pyk2/RAFTK-associated protein, SKAP-HOM, ...
Enrichment and analysis of intact phosphoproteins in arabidopsis seedlings - NRC Publications Archive - Canada.ca
Enrichment and analysis of intact phosphoproteins in arabidopsis seedlings ... Enrichment of intact phosphoproteins prior to 2-DE and LC-MS/MS appears to enhance detection of phosphorylated threonine and ... phosphoprotein; ribulosebisphosphate carboxylase; threonine; tyrosine; Arabidopsis thaliana; carbohydrate metabolism; ... Enrichment and analysis of intact phosphoproteins in arabidopsis seedlings. From National Research Council Canada ...
Search Results
Monoclonal antibodies against a 65-kDa tumor-associated phosphoprotein: Development and use in cancer detection<...
Wang S, Mirowski M, Sherman U, Walaszek Z, Hanausek M. Monoclonal antibodies against a 65-kDa tumor-associated phosphoprotein: ... Monoclonal antibodies against a 65-kDa tumor-associated phosphoprotein : Development and use in cancer detection. In: Hybridoma ... Monoclonal antibodies against a 65-kDa tumor-associated phosphoprotein : Development and use in cancer detection. / Wang, S.; ... Dive into the research topics of Monoclonal antibodies against a 65-kDa tumor-associated phosphoprotein: Development and use ...
Sol Genomics Network
PhosphorylationVasodilator-stimulated phoGenePhosphatasesProteinsAcidicNucleocapsidInhibitsDopamineAntibodiesNuclearExpressionHomoIdentificationSequenceStructuralDetectionHumanCellMilkCancerStudyResponseFunctionPhosphataseCAMP-regulated phosphoprotein 19Extracellular vesiclesEnrichmentPhosphatasesVASPCytoplasmic phosphoproteinPhosphorylationBindsViralAntibodyVasodilatorOverexpressionTumorBiomarkersAntibodiesDopamineProteomicsWorkflowTetramerIMACMechanismsFamilyAnalysisPresentDomainTypeBreast cancer
Phosphorylation4
- Enrichment of intact phosphoproteins prior to 2-DE and LC-MS/MS appears to enhance detection of phosphorylated threonine and tyrosine residues compared with methods that utilize peptide-level enrichment, suggesting that the two approaches are somewhat complementary in terms of phosphorylation site coverage. (canada.ca)
- Overall phosphorylation rates across significantly changed phosphoproteins were about three times higher in DFD than in normal meat. (biomedcentral.com)
- However, fast skeletal myosin light chain 2 followed by troponin T, F-actin-capping and small heat shock proteins showed the greatest phosphorylation change, and therefore they were the most important phosphoproteins underlying LT muscle conversion to DFD meat in the Rubia Gallega breed. (biomedcentral.com)
- The primary outcome measure was mean platelet reactivity index (PRI), as measured by vasodilator stimulated phosphoprotein (VASP) phosphorylation. (medscape.com)
Vasodilator-stimulated pho2
- In this light, actin-binding vasodilator-stimulated phosphoprotein (VASP) is a critical regulator of adherent cell migration, a readout of protein kinase G (PKG) activity, and a potential attractive candidate for therapeutic intervention. (ecu.edu)
- Vasodilator-stimulated phosphoprotein regulates arterial smooth muscle cell migration (Doctoral Dissertation, East Carolina University). (ecu.edu)
Gene6
- This gene is thought to encode an extracellular matrix acidic phosphoprotein that has a function in enamel mineralization during amelogenesis. (nih.gov)
- The psbB gene is located 562 bp upstream of the psbH gene for the 10 kDa phosphoprotein of photosystem II. (elsevier.com)
- The gene encoding p53 phosphoprotein, originally believed to be an oncogene, recently has been proposed as a candidate antioncogene (tumor-suppressor gene). (elsevier.com)
- 1393). Phylogenetic study based on the phosphoprotein gene of Iranian Newcastle disease viruses (NDV) isolates, 2010 -2012. (ac.ir)
- RESULTS: Phylogenetic analysis of sequences revealed that chicken and ostrich NDV isolates were closely related and placed in the genotype VII and Pigeon Paramyxovirus-1 was located in the genotype V. CONCLUSIONS:This is the first report of Phosphoprotein gene sequences of NDV strains isolated in Iran. (ac.ir)
- Identification of secreted phosphoprotein 1 gene as a new rheumatoid arthritis susceptibility gene. (cdc.gov)
Phosphatases2
- A Quantitative Chemical Proteomic Strategy for Profiling Phosphoprotein Phosphatases from Yeast to Humans. (kettenbachlab.org)
- These studies were enabled by an array of innovative technologies, including a chemical proteomicsstrategy that utilizes kinase inhibitors immobilized on beads and mass spectrometry (MS).The majority of protein dephosphorylation is carried out by phosphoprotein phosphatases (PPPs). (cancer.gov)
Proteins6
- Wildtype and mutant recombinant dentin phosphoprotein (rDPP) proteins were generated using a mammalian expression system. (bvsalud.org)
- Significantly changed phosphoproteins involved a variable number of isoforms of 13 myofibrillar and sarcoplasmic nonredundant proteins. (biomedcentral.com)
- Affinity purification of GPI anchored urokinase type plasminogen activator receptor from chrysotile exposed cells indicated that asbestos altered the profile of proteins and phosphoprotein complexes with this receptor. (cdc.gov)
- Chrysotile also changed the profile of proteins and phosphoproteins associated with FAK in intact cells. (cdc.gov)
- Immunoprecipitation and subsequent gel electrophoresis of proteins from neutrophils labeled with [γ- 32 P]ATP revealed a 170 to 190-kDa phosphoprotein specifically reactive with CD15 antibodies. (umn.edu)
- Casein (from Latin caseus, "cheese") is the name for a family of related Phosphoprotein proteins. (wordpress.com)
Acidic2
- Recombinant Human Dentin Matrix Acidic Phosphoprotein 1/DMP-1 is produced by our mammalian expression system in human cells. (antikoerper-online.de)
- Dentin Matrix Acidic Phosphoprotein 1 (DMP-1) is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. (antikoerper-online.de)
Nucleocapsid1
- Two majorproteins, coded by the viral genome, are nucleocapsid protein (N) and phosphoprotein (P). The N protein protects the viralgenomic RNA and forms ribonucleoprotein complex (RNP) together with P protein. (who.int)
Inhibits1
- Dentin phosphoprotein inhibits lipopolysaccharide-induced macrophage activation independent of its serine/aspartic acid-rich repeats. (bvsalud.org)
Dopamine2
- The drug combines potent serotonin 5-HT2A receptor antagonism, dopamine receptor phosphoprotein modulation, glutamatergic modulation, and serotonin reuptake inhibition, according to Intra- Cellular Therapies. (medscape.com)
- DARPP-32, a dopamine- and adenosine 3':5'-monophosphate-regulated phosphoprotein enriched in dopamine-innervated brain regions. (cornell.edu)
Antibodies2
- Five hybridoma cell lines secreting monoclonal antibodies (MAbs) to a 65- kDa tumor-associated phosphoprotein (p65) were established. (uthscsa.edu)
- These antibodies, as well as several other widely used mAb reactive with human neutrophils, were employed to detect phosphoproteins present on these cells. (umn.edu)
Nuclear1
- H.J. Vogel, Phosphorus-31 Nuclear Magnetic Resonance of Phosphoproteins. (elsevier.com)
Expression1
- Competent cell lines for inducible phosphoprotein expression TKB1 Competent Cells are derived from the BL21. (thomassci.com)
Homo1
- A DNA sequence encoding the Homo sapiens (Human) Stress-induced-phosphoprotein 1, was expressed in the hosts and tags indicated. (fishersci.fi)
Identification2
- Identification of a calcium-regulated insulinoma cell phosphoprotein as an islet cell keratin. (rupress.org)
- For this purpose, two-dimensional gel electrophoresis (2-DE), in-gel multiplex identification of phosphoproteins with PRO-Q Diamond phosphoprotein-specific stain, tandem (MALDI-TOF/TOF) mass spectrometry (MS), novel quantitative phosphoproteomic statistics and bioinformatic tools were used. (biomedcentral.com)
Sequence1
- Through a meta-prediction of disorder, we localized a folded C-terminal domain in the amino acid sequence of its phosphoprotein. (rcsb.org)
Structural1
- Human Parainfluenza Virus 3 Phosphoprotein Is a Tetramer and Shares Structural and Interaction Features with Ebola Phosphoprotein VP35. (oregonstate.edu)
Detection2
- Increased circulating cell signalling phosphoproteins in sera are useful forthe detection of pancreatic cancer. (nii.ac.jp)
- This assay has high sensitivity and excellent specificity for detection of Secreted Phosphoprotein 2 (SPP2). (biomatik.com)
Human1
- Recently, it was shown that this phosphoprotein is one of the major substrates of ecto-protein kinase activity on human neutrophils. (umn.edu)
Cell1
- ARPP-21 is a cyclic AMP-regulated phosphoprotein of M(r) 21 kDa that is enriched in the cell bodies and terminals of medium-sized spiny neurons in the basal ganglia. (nih.gov)
Milk1
- Phosphoproteins are available in egg yolk, milk, and bone marrow. (toppr.com)
Cancer1
- en] Malignant melanoma is a highly aggressive form of skin cancer responsible for the majority of skin cancer-related deaths. (uni.lu)
Study1
- The objective of this study was to investigate the effects of dentin phosphoprotein (DPP) on lipopolysaccharide -induced inflammatory responses of macrophages in vitro . (bvsalud.org)
Response1
- More than half of the 82 identified phosphoproteins were involved in carbohydrate metabolism, photosynthesis/respiration or oxidative stress response mechanisms. (canada.ca)
Function1
- The roles for the 170- to 190-kDa phosphoprotein and the ecto-protein kinase in neutrophil function remain to be determined. (umn.edu)
Phosphatase4
- Any process that stops or reduces the activity of a phosphoprotein phosphatase. (gsea-msigdb.org)
- The Phosphoprotein Phosphatase (PPP) family members are serine/threonine phosphatases. (flybase.org)
- Cysteamine is a Potent Inhibitor of Duodenal Phosphoprotein Phosphatase in Rats: Probable Role of Gastric Acid Hypersecretion. (routledge.com)
- Lymphocyte phosphatase-associated phosphoprotein proteoforms analyzed using monoclonal antibodies. (hcdm.org)
CAMP-regulated phosphoprotein 191
- Product Name: Human cAMP-Regulated Phosphoprotein 19 (ARP19) Antibody (APC Conjugate)Description: Purified antibody validated for. (emlinhibitor.com)
Extracellular vesicles2
- This study presents a general strategy to isolate and identify phosphoproteins in extracellular vesicles (EVs) from human plasma as potential markers to differentiate disease from healthy states. (pnas.org)
- To get around this limitation, Iliuk and his colleagues looked for phosphoproteins in circulating extracellular vesicles (EVs) like microvesicles and exosomes, which are known to contain genetic material and proteins from their cells of origin. (genomeweb.com)
Enrichment5
- Enrichment of intact phosphoproteins prior to 2-DE and LC-MS/MS appears to enhance detection of phosphorylated threonine and tyrosine residues compared with methods that utilize peptide-level enrichment, suggesting that the two approaches are somewhat complementary in terms of phosphorylation site coverage. (canada.ca)
- The approach, which combines a microfluidics-based capture of microvesicles and exosomes with Tymora's phosphoprotein enrichment reagents, could allow researchers to profile tumor signaling pathways via blood or urine samples, similar to how liquid biopsies make use of ctDNA analysis, said Tymora President and CTO Anton Iliuk. (genomeweb.com)
- Iliuk cited as one key to the work a phosphoprotein enrichment method Tymora has been developing under a Phase II SBIR grant from the National Institutes of Health the company received in 2015. (genomeweb.com)
- 13. Identification of p65-associated phosphoproteins by mass spectrometry after on-plate phosphopeptide enrichment using polymer-oxotitanium films. (nih.gov)
- Comparative analysis of phytohormone-responsive phosphoproteins in Arabidopsis thaliana using TiO2-phosphopeptide enrichment and mass accuracy precursor alignment. (mpg.de)
Phosphatases3
- Protein phosphorylation is a major regulatory mechanism for many cellular functions, but no phosphoprotein in biofluids has been developed for disease diagnosis because of the presence of active phosphatases. (pnas.org)
- Protein phosphorylation, which plays a key role in most cellular activities, is a reversible process mediated by protein kinases and phosphoprotein phosphatases. (embl.de)
- Phosphoprotein phosphatases catalyse the reverse process. (embl.de)
VASP2
- The vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena/VASP-family, regulating actin and cytoskeleton dynamics. (biomedcentral.com)
- N. P. Brindle, M. R. Holt, J. E. Davies, C. J. Price, D. R. CritchleyThe focal-adhesion vasodilator-stimulated phosphoprotein (VASP) binds to the proline-rich domain in vinculinIn Biochem J, volume 318 ( Pt 3), 1996. (fau.de)
Cytoplasmic phosphoprotein2
- also DOC-2/Differentially-expressed in Ovarian Carcinoma and p96) is a cytoplasmic phosphoprotein. (rndsystems.com)
- A cytoplasmic phosphoprotein involved in cytoskeletal regulation. (abcam.com)
Phosphorylation6
- The state of protein phosphorylation can be a key determinant of cellular physiology such as early-stage cancer, but the development of phosphoproteins in biofluids for disease diagnosis remains elusive. (pnas.org)
- Background: Phosphorylation condition of dopamine- and cAMP-regulated phosphoprotein, molecular pounds 32kDa (DARPP32) is vital to comprehend drug-mediated synaptic plasticity. (hsp90-inhibitor.com)
- Oxidative stress impairs cGMP-dependent protein kinase activation and vasodilator-stimulated phosphoprotein serine-phosphorylation. (uh.edu)
- 2009). Using this approach we have identified many kinase client relationships, including three different protein kinases responsible for over ten phosphorylation events on a single phosphoprotein. (upsc.se)
- CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. (thermofisher.com)
- ARPP-21, a cAMP-regulated phosphoprotein enriched in dopamine-innervated brain regions: tissue distribution and regulation of phosphorylation in rat brain. (cornell.edu)
Binds1
- Mononegavirales encode a viral phosphoprotein (P) that binds both the viral polymerase (L) and the viral nucleoprotein (N). The P protein acts as a molecular chaperone and is required for the formation of a functional replication/transcription complex. (nih.gov)
Viral2
- Phosphoprotein (P) of negative sense RNA viruses functions as a transcriptional transactivator of the viral polymerase (L). We report here the characterization of oligomeric P protein of rinderpest virus (RPV) and provide a structural basis for its multimerization. (iisc.ac.in)
- CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. (microarraystation.com)
Antibody2
- Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Acidic Salivary Proline Rich Phosphoprotein 2 (PRH2) in tissue homogenates, cell lysates and other biological fluids. (myelisakit.com)
- Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Acidic Salivary Proline Rich Phosphoprotein 2 (PRH2) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (myelisakit.com)
Vasodilator2
Overexpression1
- Overexpression of Secreted Phosphoprotein 1 (SPP1) predicts poor survival in HPV positive cervical cancer. (cdc.gov)
Tumor1
- Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. (fishersci.com)
Biomarkers1
- This study demonstrates that the development of phosphoproteins in plasma EVs as disease biomarkers is highly feasible and may transform cancer screening and monitoring. (pnas.org)
Antibodies1
- 1 X 96-well Filter Plate, x-Plex assay format that includes premixed coupled beads, detection antibodies, and lysates for detecting total Phosphoprotein non-magnetic MEK1 (Ser217/Ser221), NF-kappaB p65 (Ser536), p38 MAPK (Thr180/Tyr182), requires phosphoprotein reagent and cell lysis kit offered separately. (bio-rad.com)
Dopamine2
- Although Dopamine and cAMP-regulated phosphoprotein, Mr 32000 (DARPP-32) is overexpressed in two-thirds of gastric cancers, its impact on molecular functions has not been fully characterized. (aacrjournals.org)
- ARPP-21, a cyclic AMP-regulated phosphoprotein enriched in dopamine-innervated brain regions. (cornell.edu)
Proteomics1
- Our Orbitrap Fusion is available to support TMT based quantitative proteomics experiments, including global analysis of phosphoproteins, and we've included some example prices for TMT analysisin the cost section. (ohsu.edu)
Workflow1
- The other aspect, which Iliuk and his colleagues have developed since the work presented in the PNAS study, is an improved workflow for capturing target EVs and extracting the phosphoproteins inside. (genomeweb.com)
Tetramer1
- The data reveal the phosphoprotein to assemble into a stable tetramer, with the regions outside the oligomerization domain remaining highly flexible. (nih.gov)
IMAC1
- Tsai TL、 Wang V、 Hsiao HA、 Lu JF、 Chen HM,「Evaluation of the chromatographic parameters affecting the purification efficacy in IMAC based phosphoprotein purification: Is there a universal IMAC condition? (fju.edu.tw)
Mechanisms1
- More than half of the 82 identified phosphoproteins were involved in carbohydrate metabolism, photosynthesis/respiration or oxidative stress response mechanisms. (canada.ca)
Family1
- src family associated phosphoprotein 1.SKAP55.src family associated phosphoprotein 1. (stanford.edu)
Analysis2
- Analysis by partial proteolysis indicated that it is related to a 23-kDa phosphoprotein present in H2ts-1 virions assembled in infected cells maintained at 39°C. Affinity labeling with [ 3 H]diisopropyl fluorophosphate showed that the 19-kDa protein contains the active site for a serine protease. (princeton.edu)
- 8. Phosphoproteins analysis in plants: a proteomic approach. (nih.gov)
Present1
- The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample. (perkinelmer.com)
Domain1
- In this study, the structure of the oligomerization domain of the phosphoprotein determined by X-ray crystallography is reported. (nih.gov)
Type1
- Chatterjee, PK & Flint, SJ 1987, ' Adenovirus type 2 endopeptidase: An unusual phosphoprotein enzyme matured by autocatalysis ', Proceedings of the National Academy of Sciences of the United States of America , vol. 84, no. 3, pp. 714-718. (princeton.edu)
Breast cancer2
- We identified close to 10,000 unique phosphopeptides in EVs from small volumes of plasma samples and more than 100 phosphoproteins in plasma EVs that are significantly higher in patients diagnosed with breast cancer as compared with healthy controls. (pnas.org)
- Expression and clinicopathological significance of oestrogen-responsive ezrin-radixin-moesin-binding phosphoprotein 50 in breast cancer. (nih.gov)