Bacteriophage phi X 174: The type species of the genus MICROVIRUS. A prototype of the small virulent DNA coliphages, it is composed of a single strand of supercoiled circular DNA, which on infection, is converted to a double-stranded replicative form by a host enzyme.DNA, Viral: Deoxyribonucleic acid that makes up the genetic material of viruses.DNA Replication: The process by which a DNA molecule is duplicated.Coliphages: Viruses whose host is Escherichia coli.DNA, Single-Stranded: A single chain of deoxyribonucleotides that occurs in some bacteria and viruses. It usually exists as a covalently closed circle.Bacteriophage phi 6: Virulent bacteriophage and sole member of the genus Cystovirus that infects Pseudomonas species. The virion has a segmented genome consisting of three pieces of doubled-stranded DNA and also a unique lipid-containing envelope.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Viral Proteins: Proteins found in any species of virus.Peptide PHI: A 27-amino acid peptide with histidine at the N-terminal and isoleucine amide at the C-terminal. The exact amino acid composition of the peptide is species dependent. The peptide is secreted in the intestine, but is found in the nervous system, many organs, and in the majority of peripheral tissues. It has a wide range of biological actions, affecting the cardiovascular, gastrointestinal, respiratory, and central nervous systems.DNA, Circular: Any of the covalently closed DNA molecules found in bacteria, many viruses, mitochondria, plastids, and plasmids. Small, polydisperse circular DNA's have also been observed in a number of eukaryotic organisms and are suggested to have homology with chromosomal DNA and the capacity to be inserted into, and excised from, chromosomal DNA. It is a fragment of DNA formed by a process of looping out and deletion, containing a constant region of the mu heavy chain and the 3'-part of the mu switch region. Circular DNA is a normal product of rearrangement among gene segments encoding the variable regions of immunoglobulin light and heavy chains, as well as the T-cell receptor. (Riger et al., Glossary of Genetics, 5th ed & Segen, Dictionary of Modern Medicine, 1992)Genes, Viral: The functional hereditary units of VIRUSES.Templates, Genetic: Macromolecular molds for the synthesis of complementary macromolecules, as in DNA REPLICATION; GENETIC TRANSCRIPTION of DNA to RNA, and GENETIC TRANSLATION of RNA into POLYPEPTIDES.DNA Restriction Enzymes: Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Virus Replication: The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.Bacteriophages: Viruses whose hosts are bacterial cells.DNA Viruses: Viruses whose nucleic acid is DNA.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.DNA, Superhelical: Circular duplex DNA isolated from viruses, bacteria and mitochondria in supercoiled or supertwisted form. This superhelical DNA is endowed with free energy. During transcription, the magnitude of RNA initiation is proportional to the DNA superhelicity.Bacillus Phages: Viruses whose host is Bacillus. Frequently encountered Bacillus phages include bacteriophage phi 29 and bacteriophage phi 105.Lysogeny: The phenomenon by which a temperate phage incorporates itself into the DNA of a bacterial host, establishing a kind of symbiotic relation between PROPHAGE and bacterium which results in the perpetuation of the prophage in all the descendants of the bacterium. Upon induction (VIRUS ACTIVATION) by various agents, such as ultraviolet radiation, the phage is released, which then becomes virulent and lyses the bacterium.Kinetics: The rate dynamics in chemical or physical systems.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.DNA, Recombinant: Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.Deoxyribonucleotides: A purine or pyrimidine base bonded to a DEOXYRIBOSE containing a bond to a phosphate group.X Chromosome: The female sex chromosome, being the differential sex chromosome carried by half the male gametes and all female gametes in human and other male-heterogametic species.Phosphorus Radioisotopes: Unstable isotopes of phosphorus that decay or disintegrate emitting radiation. P atoms with atomic weights 28-34 except 31 are radioactive phosphorus isotopes.Endonucleases: Enzymes that catalyze the hydrolysis of the internal bonds and thereby the formation of polynucleotides or oligonucleotides from ribo- or deoxyribonucleotide chains. EC 3.1.-.RNA Nucleotidyltransferases: Enzymes that catalyze the template-directed incorporation of ribonucleotides into an RNA chain. EC 2.7.7.-.DNA-Directed DNA Polymerase: DNA-dependent DNA polymerases found in bacteria, animal and plant cells. During the replication process, these enzymes catalyze the addition of deoxyribonucleotide residues to the end of a DNA strand in the presence of DNA as template-primer. They also possess exonuclease activity and therefore function in DNA repair.DNA Primase: A single-stranded DNA-dependent RNA polymerase that functions to initiate, or prime, DNA synthesis by synthesizing oligoribonucleotide primers. EC 2.7.7.-.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Deoxyribonucleoproteins: Proteins conjugated with deoxyribonucleic acids (DNA) or specific DNA.DNA Polymerase I: A DNA-dependent DNA polymerase characterized in prokaryotes and may be present in higher organisms. It has both 3'-5' and 5'-3' exonuclease activity, but cannot use native double-stranded DNA as template-primer. It is not inhibited by sulfhydryl reagents and is active in both DNA synthesis and repair. EC 2.7.7.7.Nucleic Acid Conformation: The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.Deoxyribonucleases: Enzymes which catalyze the hydrolases of ester bonds within DNA. EC 3.1.-.Molecular Weight: The sum of the weight of all the atoms in a molecule.Genes: A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.Adsorption: The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily.Electrophoresis, Agar Gel: Electrophoresis in which agar or agarose gel is used as the diffusion medium.Apurinic Acid: Hydrolysate of DNA in which purine bases have been removed.Pregnancy: The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.Streptococcus agalactiae: A bacterium which causes mastitis in cattle and occasionally in man.Biological Therapy: Treatment of diseases with biological materials or biological response modifiers, such as the use of GENES; CELLS; TISSUES; organs; SERUM; VACCINES; and humoral agents.Biological Control Agents: Organisms, biological agents, or biologically-derived agents used strategically for their positive or adverse effect on the physiology and/or reproductive health of other organisms.Pseudomonas Phages: Viruses whose host is Pseudomonas. A frequently encountered Pseudomonas phage is BACTERIOPHAGE PHI 6.Metagenome: A collective genome representative of the many organisms, primarily microorganisms, existing in a community.Factor VIII: Blood-coagulation factor VIII. Antihemophilic factor that is part of the factor VIII/von Willebrand factor complex. Factor VIII is produced in the liver and acts in the intrinsic pathway of blood coagulation. It serves as a cofactor in factor X activation and this action is markedly enhanced by small amounts of thrombin.Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.Hemophilia A: The classic hemophilia resulting from a deficiency of factor VIII. It is an inherited disorder of blood coagulation characterized by a permanent tendency to hemorrhage.Drug Contamination: The presence of organisms, or any foreign material that makes a drug preparation impure.Inventions: A novel composition, device, or process, independently conceived de novo or derived from a pre-existing model.von Willebrand Diseases: Group of hemorrhagic disorders in which the VON WILLEBRAND FACTOR is either quantitatively or qualitatively abnormal. They are usually inherited as an autosomal dominant trait though rare kindreds are autosomal recessive. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII, and impaired platelet adhesion.Acquired Immunodeficiency Syndrome: An acquired defect of cellular immunity associated with infection by the human immunodeficiency virus (HIV), a CD4-positive T-lymphocyte count under 200 cells/microliter or less than 14% of total lymphocytes, and increased susceptibility to opportunistic infections and malignant neoplasms. Clinical manifestations also include emaciation (wasting) and dementia. These elements reflect criteria for AIDS as defined by the CDC in 1993.
PHI Learning Pvt. Ltd. pp. 393-394. ISBN 978-81-203-3055-9. Retrieved 25 March 2011. Quinlan, Philip; Dyson, Ben (2008). ... 173-174. ISBN 978-1-86105-586-6. Retrieved 24 March 2011. Mangal, S. K. (1 August 2007). Essentials of educational psychology. ...
He is a member of the Latino fraternity Phi Iota Alpha. The oldest inter-collegiate Greek-letter organization established for ... 174 ed.). United States Department of State, Bureau of Public Affairs. January 1988. pp. 42-43. ...
Phage lambda Phi X 174 - its genome was the first ever to be sequenced. The genome is a circle of 11 genes, 5386 base pairs in ...
University), Phi Beta Kappa Massachusetts Alpha (Harvard (1912). Catalogue of the Harvard Chapter of Phi Beta Kappa, Alpha of ... from which he graduated Phi Beta Kappa in 1782. On his 21st birthday, Van Rensselaer took possession of Rensselaerswyck, his ... p. 174. ISBN 978-0-521-86196-0. Bielinski, Stefan. "Stephen Van Rensselaer III", nysm.nysed.gov; accessed April 15, 2016. Fitch ...
In 2003 the same group synthetically assembled the genome of a virus, Phi X 174 bacteriophage. Currently, Smith is scientific ...
Randall was a member of the Phi Beta Kappa Society. In 1896, he served as a coach at the University of Illinois under fellow ... Phi Beta Kappa Handbook and General Address Catalogue of the United Chapters, p. 89, c. 1904. The Dartmouth, p. 322. ... The Dartmouth, Volume 30, p. 174, 1908. 2008 Football Media Guide (PDF), p. 139, Dartmouth College, 2008. ...
PHI Learning. Retrieved 10 July 2011. International Electrotechnical Commission (2007). "Prefixes for binary multiples". ... CMU-CS-99-174). CARNEGIE-MELLON UNIV PITTSBURGH PA SCHOOL OF COMPUTER SCIENCE. "Monitoring file transfers that are in progress ...
PCA was used to generate the first synthetic genome in history, that of the Phi X 174 virus. The Gibson assembly method, ...
Sanger and his team of scientists created a library of the bacteriophage, phi X 174, for use in DNA sequencing. The importance ... February 1977). "Nucleotide sequence of bacteriophage phi X174 DNA". Nature. 265 (5596): 687-95. doi:10.1038/265687a0. PMID ...
In 2003 a faster method was shown to assemble the 5386-base genome of the bacteriophage Phi X 174 in 2 weeks. The giant ... In 1977, Frederick Sanger achieved the first complete sequencing of the genome of any organism, the bacteriophage Phi X 174. In ...
He is a member of Phi Beta Sigma fraternity. Jackson was also a member of the Miami Dolphins, New England Patriots, and ... Jackson started 21 of 47 games at both the cornerback and strong safety slots for the University of Arkansas and totaled 174 ...
"An Alternative Form of Collective Bargaining." Phi Delta Kappan 67 (1986): 605-607. Alberta Human Rights Act, RSA 2000, c A-25 ... "Collective Gaining: A Bargaining Alternative." Phi Delta Kappan 63 (1981): 244-245. DeGennaro, William, and Kay Michelfeld. " ... "Fix on These Five Guiding Lights, and Emerge from the Bargaining Fog." The American School Board Journal 174 (1987): 31. ... "Prescription for Labor Pains: Combine Bargaining with Problem Solving." The American School Board Journal 174 (1987): 24. ...
... bacteriophage phi 6 MeSH B04.123.691.230 --- cystoviridae MeSH B04.123.691.230.070 --- bacteriophage phi 6 MeSH B04.123.691.600 ... bacteriophage phi x 174 MeSH B04.280.505.900 --- white spot syndrome virus 1 MeSH B04.280.535.600 --- papillomavirus MeSH ... bacteriophage phi x 174 MeSH B04.123.205.350 --- bacteriophage prd1 MeSH B04.123.205.600 --- leviviridae MeSH B04.123.205.600. ... bacteriophage phi 6 MeSH B04.820.250.350 --- flavivirus MeSH B04.820.250.350.270 --- dengue virus MeSH B04.820.250.350.300 --- ...
... phi}X174 bacteriophage from synthetic oligonucleotides". Proceedings of the National Academy of Sciences. 100 (26): 15440-15445 ... producing the 5386-base genome of the bacteriophage Phi X 174 in about two weeks. However, the genome of Mycoplasma ...
... on parchment Phi X 174 bacteriophage was the first DNA-based genome to be sequenced VA-174 List of highways numbered 174 United ... 174 is an even number, a composite number, a sphenic number, and an abundant number with the abundance of 12. It is a ... 174 Phaedra is a large, rocky main belt asteroid Gliese 174 or V834 Tau, a variable star 174th Air Refueling Squadron unit of ... 174 174 in the New York City Subway System The former 174th Street elevated station on the demolished IRT Third Avenue Line. ...
"Hybridization of synthetic oligodeoxyribonucleotides to Phi-X 174 DNA: the effect of single base pair mismatch." Nucleic Acids ...
G4 phage P1 phage Enterobacteria phage P2 P4 phage Phi X 174 phage N4 phage Pseudomonas phage Φ6 Φ29 phage 186 phage Viruses ...
... in patients with antibody deficiencies and the in vivo analysis of antibody production using bacteriophage Phi X 174. He and ...
Xeon Phi processors, while it debuted in November 2015 at 6th. Sierra (supercomputer) - ATS-2, the second Advanced Technology ... System "Cray Awarded $174 Million Supercomputer Contract From the National Nuclear Security Administration". Retrieved 2014-08- ...
... and her PhD in 1975 from the University of Cambridge in molecular biology on the bacteriophage Phi X 174 while a student of ...
... it was only in 1959 that Sinshemer working with phage Phi X 174 showed that they could possess single-stranded DNA genomes. ...
Soder, Roger (June 2005). "Books for Summer Reading". Phi Delta Kappan. 86 (10): 777. doi:10.1177/003172170508601012.. ... 174 (9): 1307-1308. doi:10.1503/cmaj.060083. PMC 1435967.. *^ Ellis, David S. J. (February 2004). "The Curious Incident of the ...
Phi Delta Kappan, 96(8), 38-42. doi: 10.1177/0031721715583961 Conyers, M. A., & Wilson, D. L. (2009). Introduction to ... Information Age Education Newsletter, 174. Conyers, M. A. (November, 2014). Education for students' futures. Part 16: ...
C. M. Bender; S. S. Pinsky; B. van de Sande (1993). "Spontaneous symmetry breaking of ϕ 4 {\displaystyle \phi ^{4}} in (1+1)- ... V. T. Kim; G. B. Pivovarov; J. P. Vary (2004). "Phase transition in light front ϕ 1 + 1 4 {\displaystyle \phi _{1+1}^{4}} ". ... S. S. Pinsky; B. van de Sande (1994). "Spontaneous symmetry breaking of (1+1)-dimensional ϕ 4 {\displaystyle \phi ^{4}} theory ... phi ^{4}} theory, have their counterparts in zero modes or, possibly, in additional terms in the LFQCD Hamiltonian that are ...
He was elected to honorary societies of Phi Beta Kappa, Phi Delta Kappa, Delta Sigma Rho, and Pi Gamma Mu. He was a member of ... pp.174". "CORNELL ALUMNI NEWS Volume 36 No.32 June 21, 1934 on Cornellian Council Elects Becker, Palmer" (PDF). "Law and ...
"Whatever happened to cold fusion?", American Scholar, Phi Beta Kappa Society, 63 (4): 527-541, ISSN 0003-0937, retrieved 25 ... 174] On 2 December 1993 the University of Utah licensed all its cold fusion patents to ENECO, a new company created to profit ...
The phi X 174 (or ΦX174) bacteriophage is a single-stranded DNA (ssDNA) virus that infects Escherichia coli, and the first DNA- ... Phi X is regularly used as a positive control in DNA sequencing due to its relatively small genome size in comparison to other ... Retrieved from "https://en.wikipedia.org/w/index.php?title=Phi_X_174&oldid=855147223" ...
Geiger, L.E.; Morris, D.R., 1980: Specificity of the spermidine requirement for the replication of phi X174 DNA be cell-free ... Geiger L.E.; Morris D.R., 1980: Specificity of the spermidine requirement for the replication of phage phi x 174 dna by cell ... of spermidine interactions in isolated systems is applied to the stimulation by spermidine of the conversion of phi X174 virion ...
174 mutants in tsDNA mutants Growth studies of three phi chi by Stephen Haworth et al. ... Escherichia coli . 174 mutants in tsDNA mutants Growth studies of three phi chi. @inproceedings{Haworth2003EscherichiaC, title ... Escherichia coli . 174 mutants in tsDNA mutants Growth studies of three phi chi}, author={Stephen Haworth and C F Gilgun and ...
See List of Phi Delta Theta members Famous Phis Members of Phi Delta Theta have held numerous political positions in the United ... "Phi Delta International Site - History". Phi Delta Theta. Retrieved 2008-03-01. "Phi Delta Theta Housing Corporation Value" ( ... published by Phi Delta Theta (2002) "The Pursuant Group, Inner Shield". Phi Delta Theta. Retrieved 2007-12-13. "Phi Delta Theta ... Fifty Phis fought on the side of the Confederacy while 231 Phis fought for the Union Army. It was not until the 1868 ...
Bacteriophage Phi X 174‎ (6 B). *. ► Bacteriophage trimeric proteins domain‎ (1 C) ...
The Use of Bacteriophage Phi X174 to Assess the Immune Competence of HIV-Infected Patients In Vivo. The safety and scientific ... Bacteriophage phi X174 immunization is a method that has been in use for more than 25 years to assess the immunity of patients ... Bacteriophage phi X174 immunization is a method that has been in use for more than 25 years to assess the immunity of patients ... The objective of this study is to evaluate the safety and utility of bacteriophage phi X174 immunization as a tool to assess ...
Virus : Bacteriophage (Phi X 174 NBRC 103405). • Test Report number : 11047937001-02. Mould : Cladosporium cladosporioides ( ... Virus : Escherichia coli phage (φΧ-174 ATCC 13706-B1). All results are based on specific testing conditions.. All tests are not ... Virus : Escherichia coli phage (øX-174 ATCC 13706-B1). : Influenza (H1N1) 2009 Virus. • KRCES-Env. Test Report no. : 23_0140. ...
PHI. NL. 127. 577. 545. 107. 197. 40. 23. 9. 127. 20. 15. 25. .361. .392. .569. .960. 155. 310. 12. 5. *3O/5. ...
PHI. M. 2. 2. 174. 0. 0. 5. 1. 0. 0/0. 0. 0. 0.00. 0.0. ...
PHI. M. 2. 0. 42. 0. 0. 0. 0. 0. 0/0. 0. 0. 0.00. 0.0. ...
Bacteriophage phi X 174 / genetics* * DNA, Single-Stranded / genetics* * Epistasis, Genetic * Evolution, Molecular ...
Pyrophosphate enhances misincorporation by Escherichia coli DNA polymerase I in copying phi X174 DNA. The increased ... Pyrophosphate enhances misincorporation by Escherichia coli DNA polymerase I in copying phi X174 DNA. The increased ...
hemocyanin (KLH) and Bacteriophage Phi-X 174 (PhiX174) to evaluate antigen-specific B and T. cell responses. Clinical response ...
1954 Philadelphia Eagles Statistics & Players: 7-4-1 (2nd in East), Coach: Jim Trimble, ProBowl: Bednarik, Burk, Kilroy, Pihos, Robinson, Snyder, Willey
Uhr, J. W., Dancis, J., and Finkelstein, M. S. (1963). Passage of bacteriophage phi X 174 across the placenta in guinea pigs. ... Ochs, H. D., Davis, S. D., and Wedgwood, R. J. (1971). Immunologic responses to bacteriophage phi-X 174 in immunodeficiency ...
T7 and Phi X 174. Further, mixtures of the viruses may also be used. ...
Or about 300 times the size of the Phi X genome. Five years later, we upped the ante another 1,600 times with the first draft ... We decided to start where DNA history started, with Phi X 174. We chose it as a test synthesis because you can make very few ... This is a picture of Sangers team that sequenced Phi X 174. The second guy from the left on the bottom is Clyde Hutchison, who ... The human genome is about a half a million times larger the Phi X genome, so it shows how fast things were developing. Reading ...
X-linked lymphoproliferative syndrome: abnormal antibody responses to bacteriophage phi X 174. Birth Defects Orig Artic Ser ( ...
Supported by grant funds from the Institute of Museum and Library Services under the provisions of the federal Library Services and Technology Act (LSTA) as administered by the State Library of North Carolina, a division of the Department of Cultural Resources.. ...
Bacteriophage Phi X 174 Electron micrograph. Credit: Wikipedia/CC BY-SA 4.0 ...
Sanger and his team of scientists created a library of the bacteriophage, phi X 174, for use in DNA sequencing. The importance ... February 1977). "Nucleotide sequence of bacteriophage phi X174 DNA". Nature. 265 (5596): 687-95. doi:10.1038/265687a0. PMID ...
615 : // If a phi node uses a register paired with a subregister, the actual 616 : // value type of the phi may differ from ... phi(1, 3) in 2, it has to be ultimately replaced 937 : // with a phi in 3 (which now dominates 2). 938 : // What we do here is ... 890 972 : while (I != TailBB-,end() && I-,isPHI()) { 891 : // Replace the uses of the def of the PHI with the register coming ... 953 0 : while (I != TailBB-,end() && I-,isPHI()) { 954 : // Replace the uses of the def of the PHI with the register coming 955 ...
... phi = true; 250 } 251 } 252 assert(!has_phi ,, rgn-,req() , 3, "no phis when region is created"); 253 254 return if_cont-,as_ ... phi = true; 175 } 176 } 177 assert(!has_phi ,, rgn-,req() , 3, "no phis when region is created"); 178 179 if (new_entry == NULL ... 166 assert(rgn-,in(rgn-,req() -1) == if_uct, "new edge should be last"); 167 bool has_phi = false; 168 for (DUIterator_Fast ... 242 assert(rgn-,in(rgn-,req() -1) == if_uct, "new edge should be last"); 243 bool has_phi = false; 244 for (DUIterator_Fast ...
Phi-X174 phage. The phi X 174 bacteriophage was the first DNA-based genome to be sequenced. This work was completed by Fred ... Phi-X174 phage. The phi X 174 bacteriophage was the first DNA-based genome to be sequenced. This work was completed by Fred ... In 1962, Walter Fiers had already demonstrated the physical, covalently closed circularity of phi X 174 DNA.In 2003, it was ... In 1962, Walter Fiers had already demonstrated the physical, covalently closed circularity of phi X 174 DNA.In 2003, it was ...
Hind II digested lambda and Hae III digested phi φ×174 DNA were used for molecular weight markers. ... Hind III digested lambda DNA and Hae III digested φ×174 were used for molecular weight markers. ... 174 DNA: 1.35 Kb, 1.08 Kb, 0.87 Kb, 0.60 Kb, 0.28 Kb and 0.23 Kb, respectively. Lanes 2 and 3 are undigested K562 DNA, lanes 4 ...
  • Isolation and investigation of the replicating function in the coding chain of the replicative form of the DNA of phage phi-X-174. (molecularstation.com)
  • When we started, we looked at phages that infect bacteria, and there was one phage we looked at-it was called Phi X 174-and it has one methyl group and we tried to understand its role. (jeffreybland.com)
  • In protest against the president of the university, members of Beta Theta Pi and another fraternity, Alpha Delta Phi, blocked the entrances of the main educational and administrative building in what became known as the Great Snowball Rebellion of 1848. (wikipedia.org)
  • alpha delta phi entry in the 1949 inter-fraternity play contest. (dartmouth.edu)
  • Phi X is regularly used as a positive control in DNA sequencing due to its relatively small genome size in comparison to other organisms, its relatively balanced nucleotide content - about 23% G, 22% C, 24% A, and 31% T, i.e., 45% G+C and 55% A+T, see the accession NC_001422.1 for its 5,386 nucleotide long sequence -, and the extensive work that has been done on it. (wikipedia.org)
  • The phi X bacteriophage is of interest because it was the first DNA-based genome to be sequenced. (bryanmbrandenburg.com)
  • The protein expressed from the S. typhimurium rol gene was found to regulate the distribution of the O75 O polysaccharide on the lipopolysaccharide of the host strain, E. coli S phi 874. (asm.org)
  • Here, we present six libraries of discrete RNA conformations based on a simplified pseudo-torsional notation of the RNA backbone, comparable to phi and psi in the protein backbone. (nih.gov)
  • Since then, the GOJoven alumni fellows and their allies in Belize, Guatemala, Honduras, and Mexico have trained an additional 174 young leaders by adapting and using the GOJoven Model. (phi.org)
  • In this post, Mark reflects on his recent trips to PHI's central offices in Oakland California, where he met with PHI staff and other health experts. (phi.org)
  • Another biomarker assay called PHI (Prostate Health index) uses a formula that incorporates pro-PSA, fPSA, and PSA for predicting PCa. (jcancer.org)
  • But exploration of the Economic Science is not the only end towards which Doctor Ever- ett has labored, for under his guidance has corne a search for knowledge, wherever it is to be found, by instilling inquisitive en- thusiasm in our minds. (e-yearbook.com)
  • Therefore, to efficiently study a number of gene products associated with a disease, pathway, or other biological process, it is necessary to be able to design primer pairs or oligonucleotides en masse rather than using a time consuming and laborious gene-by-gene method. (biomedcentral.com)
  • Aliquots from each time point are electrophoresed on an agarose gel versus the phi-X 174 size marker (12) to determine the approximate DNA fragment size range for each sonication time point. (protocol-online.org)