PeroxidasesHorseradish Peroxidase: An enzyme isolated from horseradish which is able to act as an antigen. It is frequently used as a histochemical tracer for light and electron microscopy. Its antigenicity has permitted its use as a combined antigen and marker in experimental immunology.Glutathione Peroxidase: An enzyme catalyzing the oxidation of 2 moles of glutathione in the presence of hydrogen peroxide to yield oxidized glutathione and water. EC 1.11.1.9.Peroxidase: A hemeprotein from leukocytes. Deficiency of this enzyme leads to a hereditary disorder coupled with disseminated moniliasis. It catalyzes the conversion of a donor and peroxide to an oxidized donor and water. EC 1.11.1.7.Cytochrome-c Peroxidase: A hemeprotein which catalyzes the oxidation of ferrocytochrome c to ferricytochrome c in the presence of hydrogen peroxide. EC 1.11.1.5.Ascorbate Peroxidases: Peroxidases that utilize ASCORBIC ACID as an electron donor to reduce HYDROGEN PEROXIDE to WATER. The reaction results in the production of monodehydroascorbic acid and DEHYDROASCORBIC ACID.Eosinophil Peroxidase: A 66-kDa peroxidase found in EOSINOPHIL granules. Eosinophil peroxidase is a cationic protein with a pI of 10.8 and is comprised of a heavy chain subunit and a light chain subunit. It possesses cytotoxic activity towards BACTERIA and other organisms, which is attributed to its peroxidase activity.Iodide Peroxidase: A hemeprotein that catalyzes the oxidation of the iodide radical to iodine with the subsequent iodination of many organic compounds, particularly proteins. EC 1.11.1.8.Hydrogen Peroxide: A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials.Guaiacol: An agent thought to have disinfectant properties and used as an expectorant. (From Martindale, The Extra Pharmacopoeia, 30th ed, p747)Catalase: An oxidoreductase that catalyzes the conversion of HYDROGEN PEROXIDE to water and oxygen. It is present in many animal cells. A deficiency of this enzyme results in ACATALASIA.Selenium: An element with the atomic symbol Se, atomic number 34, and atomic weight 78.96. It is an essential micronutrient for mammals and other animals but is toxic in large amounts. Selenium protects intracellular structures against oxidative damage. It is an essential component of GLUTATHIONE PEROXIDASE.Lactoperoxidase: An enzyme derived from cow's milk. It catalyzes the radioiodination of tyrosine and its derivatives and of peptides containing tyrosine.Peroxiredoxins: A family of ubiquitously-expressed peroxidases that play a role in the reduction of a broad spectrum of PEROXIDES like HYDROGEN PEROXIDE; LIPID PEROXIDES and peroxinitrite. They are found in a wide range of organisms, such as BACTERIA; PLANTS; and MAMMALS. The enzyme requires the presence of a thiol-containing intermediate such as THIOREDOXIN as a reducing cofactor.Lignin: The most abundant natural aromatic organic polymer found in all vascular plants. Lignin together with cellulose and hemicellulose are the major cell wall components of the fibers of all wood and grass species. Lignin is composed of coniferyl, p-coumaryl, and sinapyl alcohols in varying ratios in different plant species. (From Merck Index, 11th ed)Oxidation-Reduction: A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).Superoxide Dismutase: An oxidoreductase that catalyzes the reaction between superoxide anions and hydrogen to yield molecular oxygen and hydrogen peroxide. The enzyme protects the cell against dangerous levels of superoxide. EC 1.15.1.1.Chloride Peroxidase: An enzyme that catalyzes the chlorination of a range of organic molecules, forming stable carbon-chloride bonds. EC 1.11.1.10.Basidiomycota: A phylum of fungi that produce their sexual spores (basidiospores) on the outside of the basidium. It includes forms commonly known as mushrooms, boletes, puffballs, earthstars, stinkhorns, bird's-nest fungi, jelly fungi, bracket or shelf fungi, and rust and smut fungi.Benzyl Alcohols: Alcohols derived from the aryl radical (C6H5CH2-) and defined by C6H5CHOH. The concept includes derivatives with any substituents on the benzene ring.Antioxidants: Naturally occurring or synthetic substances that inhibit or retard the oxidation of a substance to which it is added. They counteract the harmful and damaging effects of oxidation in animal tissues.Glutathione Reductase: Catalyzes the oxidation of GLUTATHIONE to GLUTATHIONE DISULFIDE in the presence of NADP+. Deficiency in the enzyme is associated with HEMOLYTIC ANEMIA. Formerly listed as EC 1.6.4.2.Oxidative Stress: A disturbance in the prooxidant-antioxidant balance in favor of the former, leading to potential damage. Indicators of oxidative stress include damaged DNA bases, protein oxidation products, and lipid peroxidation products (Sies, Oxidative Stress, 1991, pxv-xvi).Heme: The color-furnishing portion of hemoglobin. It is found free in tissues and as the prosthetic group in many hemeproteins.Peroxides: A group of compounds that contain a bivalent O-O group, i.e., the oxygen atoms are univalent. They can either be inorganic or organic in nature. Such compounds release atomic (nascent) oxygen readily. Thus they are strong oxidizing agents and fire hazards when in contact with combustible materials, especially under high-temperature conditions. The chief industrial uses of peroxides are as oxidizing agents, bleaching agents, and initiators of polymerization. (From Hawley's Condensed Chemical Dictionary, 11th ed)Glutathione: A tripeptide with many roles in cells. It conjugates to drugs to make them more soluble for excretion, is a cofactor for some enzymes, is involved in protein disulfide bond rearrangement and reduces peroxides.Histocytochemistry: Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.Lipid Peroxidation: Peroxidase catalyzed oxidation of lipids using hydrogen peroxide as an electron acceptor.Spectrophotometry: The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.Kinetics: The rate dynamics in chemical or physical systems.Phanerochaete: A genus of fungi in the family Corticiaceae, order Stereales, that degrades lignin. The white-rot fungus Phanerochaete chrysosporium is a frequently used species in research.Benzidines: Very toxic industrial chemicals. They are absorbed through the skin, causing lethal blood, bladder, liver, and kidney damage and are potent, broad-spectrum carcinogens in most species.Coprinus: A genus of black-spored basidiomycetous fungi of the family Coprinaceae, order Agaricales; some species are edible.Iodides: Inorganic binary compounds of iodine or the I- ion.Selenoproteins: Selenoproteins are proteins that specifically incorporate SELENOCYSTEINE into their amino acid chain. Most selenoproteins are enzymes with the selenocysteine residues being responsible for their catalytic functions.Lipid Peroxides: Peroxides produced in the presence of a free radical by the oxidation of unsaturated fatty acids in the cell in the presence of molecular oxygen. The formation of lipid peroxides results in the destruction of the original lipid leading to the loss of integrity of the membranes. They therefore cause a variety of toxic effects in vivo and their formation is considered a pathological process in biological systems. Their formation can be inhibited by antioxidants, such as vitamin E, structural separation or low oxygen tension.Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate: The lectin wheatgerm agglutinin conjugated to the enzyme HORSERADISH PEROXIDASE. It is widely used for tracing neural pathways.Pleurotus: A genus of basidiomycetous fungi, family POLYPORACEAE, order POLYPORALES, that grows on logs or tree stumps in shelflike layers. The species P. ostreatus, the oyster mushroom, is a choice edible species and is the most frequently encountered member of the genus in eastern North America. (Alexopoulos et al., Introductory Mycology, 4th ed, p531)Selenious Acid: A selenium compound with the molecular formula H2SO3. It used as a source of SELENIUM, especially for patients that develop selenium deficiency following prolonged PARENTERAL NUTRITION.Free Radicals: Highly reactive molecules with an unsatisfied electron valence pair. Free radicals are produced in both normal and pathological processes. They are proven or suspected agents of tissue damage in a wide variety of circumstances including radiation, damage from environment chemicals, and aging. Natural and pharmacological prevention of free radical damage is being actively investigated.Malondialdehyde: The dialdehyde of malonic acid.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Peroxiredoxin VI: A peroxiredoxin that is a cytosolic bifunctional enzyme. It functions as a peroxiredoxin via a single redox-active cysteine and also contains a Ca2+-independent acidic phospholipase A2 activity.Catalysis: The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.Thyroid Gland: A highly vascularized endocrine gland consisting of two lobes joined by a thin band of tissue with one lobe on each side of the TRACHEA. It secretes THYROID HORMONES from the follicular cells and CALCITONIN from the parafollicular cells thereby regulating METABOLISM and CALCIUM level in blood, respectively.3,3'-DiaminobenzidineAmino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Electron Spin Resonance Spectroscopy: A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.Peroxiredoxin III: A THIOREDOXIN-dependent hydroperoxidase that is localized in the mitochondrial matrix. The enzyme plays a crucial role in protecting mitochondrial components from elevated levels of HYDROGEN PEROXIDE.Selenoprotein P: An extracellular selenoprotein that contains most of the SELENIUM in PLASMA. Selenoprotein P functions as an antioxidant and appears to transport selenium from the LIVER to peripheral tissues.Isoenzymes: Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.Hemeproteins: Proteins that contain an iron-porphyrin, or heme, prosthetic group resembling that of hemoglobin. (From Lehninger, Principles of Biochemistry, 1982, p480)Selenocysteine: A naturally occurring amino acid in both eukaryotic and prokaryotic organisms. It is found in tRNAs and in the catalytic site of some enzymes. The genes for glutathione peroxidase and formate dehydrogenase contain the TGA codon, which codes for this amino acid.Polyporaceae: A family of bracket fungi, order POLYPORALES, living in decaying plant matter and timber.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Ascorbic Acid: A six carbon compound related to glucose. It is found naturally in citrus fruits and many vegetables. Ascorbic acid is an essential nutrient in human diets, and necessary to maintain connective tissue and bone. Its biologically active form, vitamin C, functions as a reducing agent and coenzyme in several metabolic pathways. Vitamin C is considered an antioxidant.Polyporales: An order of fungi in the phylum BASIDIOMYCOTA having macroscopic basidiocarps. The members are characterized by their saprophytic activities as decomposers, particularly in the degradation of CELLULOSE and LIGNIN. A large number of species in the order have been used medicinally. (From Alexopoulos, Introductory Mycology, 4th ed, pp504-68)Reactive Oxygen Species: Molecules or ions formed by the incomplete one-electron reduction of oxygen. These reactive oxygen intermediates include SINGLET OXYGEN; SUPEROXIDES; PEROXIDES; HYDROXYL RADICAL; and HYPOCHLOROUS ACID. They contribute to the microbicidal activity of PHAGOCYTES, regulation of signal transduction and gene expression, and the oxidative damage to NUCLEIC ACIDS; PROTEINS; and LIPIDS.Thiobarbituric Acid Reactive Substances: Low-molecular-weight end products, probably malondialdehyde, that are formed during the decomposition of lipid peroxidation products. These compounds react with thiobarbituric acid to form a fluorescent red adduct.Oxidoreductases: The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)Glucose Oxidase: An enzyme of the oxidoreductase class that catalyzes the conversion of beta-D-glucose and oxygen to D-glucono-1,5-lactone and peroxide. It is a flavoprotein, highly specific for beta-D-glucose. The enzyme is produced by Penicillium notatum and other fungi and has antibacterial activity in the presence of glucose and oxygen. It is used to estimate glucose concentration in blood or urine samples through the formation of colored dyes by the hydrogen peroxide produced in the reaction. (From Enzyme Nomenclature, 1992) EC 1.1.3.4.Cyanides: Inorganic salts of HYDROGEN CYANIDE containing the -CN radical. The concept also includes isocyanides. It is distinguished from NITRILES, which denotes organic compounds containing the -CN radical.Eosinophils: Granular leukocytes with a nucleus that usually has two lobes connected by a slender thread of chromatin, and cytoplasm containing coarse, round granules that are uniform in size and stainable by eosin.Enzymes, Immobilized: Enzymes which are immobilized on or in a variety of water-soluble or water-insoluble matrices with little or no loss of their catalytic activity. Since they can be reused continuously, immobilized enzymes have found wide application in the industrial, medical and research fields.Manganese: A trace element with atomic symbol Mn, atomic number 25, and atomic weight 54.94. It is concentrated in cell mitochondria, mostly in the pituitary gland, liver, pancreas, kidney, and bone, influences the synthesis of mucopolysaccharides, stimulates hepatic synthesis of cholesterol and fatty acids, and is a cofactor in many enzymes, including arginase and alkaline phosphatase in the liver. (From AMA Drug Evaluations Annual 1992, p2035)Glutathione Transferase: A transferase that catalyzes the addition of aliphatic, aromatic, or heterocyclic FREE RADICALS as well as EPOXIDES and arene oxides to GLUTATHIONE. Addition takes place at the SULFUR. It also catalyzes the reduction of polyol nitrate by glutathione to polyol and nitrite.Agaricales: An extensive order of basidiomycetous fungi whose fruiting bodies are commonly called mushrooms.tert-Butylhydroperoxide: A direct-acting oxidative stress-inducing agent used to examine the effects of oxidant stress on Ca(2+)-dependent signal transduction in vascular endothelial cells. It is also used as a catalyst in polymerization reactions and to introduce peroxy groups into organic molecules.Armoracia: A plant genus of the family BRASSICACEAE known for the root used in hot SPICES. It is also the source of HORSERADISH PEROXIDASE which is widely used in laboratories.ThyroglobulinThiocyanates: Organic derivatives of thiocyanic acid which contain the general formula R-SCN.Selenomethionine: Diagnostic aid in pancreas function determination.Oxygen: An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.Ferrocyanides: Inorganic salts of the hypothetical acid ferrocyanic acid (H4Fe(CN)6).Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Oxidants: Electron-accepting molecules in chemical reactions in which electrons are transferred from one molecule to another (OXIDATION-REDUCTION).Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Phenols: Benzene derivatives that include one or more hydroxyl groups attached to the ring structure.Laccase: A copper-containing oxidoreductase enzyme that catalyzes the oxidation of 4-benzenediol to 4-benzosemiquinone. It also has activity towards a variety of O-quinols and P-quinols. It primarily found in FUNGI and is involved in LIGNIN degradation, pigment biosynthesis and detoxification of lignin-derived products.Spectrum Analysis, Raman: Analysis of the intensity of Raman scattering of monochromatic light as a function of frequency of the scattered light.Pinocytosis: The engulfing of liquids by cells by a process of invagination and closure of the cell membrane to form fluid-filled vacuoles.Immunoenzyme Techniques: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.Glutathione Disulfide: A GLUTATHIONE dimer formed by a disulfide bond between the cysteine sulfhydryl side chains during the course of being oxidized.Anisoles: A group of compounds that are derivatives of methoxybenzene and contain the general formula R-C7H7O.Spectrophotometry, Ultraviolet: Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Sodium Selenite: The disodium salt of selenious acid. It is used therapeutically to supply the trace element selenium and is prepared by the reaction of SELENIUM DIOXIDE with SODIUM HYDROXIDE.Amitrole: A non-selective post-emergence, translocated herbicide. According to the Seventh Annual Report on Carcinogens (PB95-109781, 1994) this substance may reasonably be anticipated to be a carcinogen. (From Merck Index, 12th ed) It is an irreversible inhibitor of CATALASE, and thus impairs activity of peroxisomes.Erythrocytes: Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.Benzene DerivativesThyroiditis, Autoimmune: Inflammatory disease of the THYROID GLAND due to autoimmune responses leading to lymphocytic infiltration of the gland. It is characterized by the presence of circulating thyroid antigen-specific T-CELLS and thyroid AUTOANTIBODIES. The clinical signs can range from HYPOTHYROIDISM to THYROTOXICOSIS depending on the type of autoimmune thyroiditis.Plants: Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.Bromides: Salts of hydrobromic acid, HBr, with the bromine atom in the 1- oxidation state. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Vitamin E: A generic descriptor for all TOCOPHEROLS and TOCOTRIENOLS that exhibit ALPHA-TOCOPHEROL activity. By virtue of the phenolic hydrogen on the 2H-1-benzopyran-6-ol nucleus, these compounds exhibit varying degree of antioxidant activity, depending on the site and number of methyl groups and the type of ISOPRENOIDS.Thioredoxins: Hydrogen-donating proteins that participates in a variety of biochemical reactions including ribonucleotide reduction and reduction of PEROXIREDOXINS. Thioredoxin is oxidized from a dithiol to a disulfide when acting as a reducing cofactor. The disulfide form is then reduced by NADPH in a reaction catalyzed by THIOREDOXIN REDUCTASE.Trametes: A genus of fungi in the family Coriolaceae.Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.Organoselenium Compounds: Organic compounds which contain selenium as an integral part of the molecule.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Thioredoxin-Disulfide Reductase: A FLAVOPROTEIN enzyme that catalyzes the oxidation of THIOREDOXINS to thioredoxin disulfide in the presence of NADP+. It was formerly listed as EC 1.6.4.5Prostaglandins G: A group of physiologically active prostaglandin endoperoxides. They are precursors in the biosynthesis of prostaglandins and thromboxanes. Most frequently encountered member of this group is the prostaglandin G2.Spectrum Analysis: The measurement of the amplitude of the components of a complex waveform throughout the frequency range of the waveform. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Models, Chemical: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Sulfhydryl Compounds: Compounds containing the -SH radical.Plant Proteins: Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.Plant Leaves: Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)Phenylhydrazines: Diazo derivatives of aniline, used as a reagent for sugars, ketones, and aldehydes. (Dorland, 28th ed)Asparagus Plant: A plant genus in the family LILIACEAE (sometimes placed in Asparagaceae) that contains ECDYSTEROIDS and is an ingredient of Siotone. The shoots are used as a vegetable and the roots are used in FOLK MEDICINE.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Coumaric Acids: Hydroxycinnamic acid and its derivatives. Act as activators of the indoleacetic acid oxidizing system, thereby producing a decrease in the endogenous level of bound indoleacetic acid in plants.Biocatalysis: The facilitation of biochemical reactions with the aid of naturally occurring catalysts such as ENZYMES.Phenol: An antiseptic and disinfectant aromatic alcohol.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Gene Expression Regulation, Enzymologic: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.Sodium Azide: A cytochrome oxidase inhibitor which is a nitridizing agent and an inhibitor of terminal oxidation. (From Merck Index, 12th ed)Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Diiodotyrosine: A product from the iodination of MONOIODOTYROSINE. In the biosynthesis of thyroid hormones, diiodotyrosine residues are coupled with other monoiodotyrosine or diiodotyrosine residues to form T4 or T3 thyroid hormones (THYROXINE and TRIIODOTHYRONINE).Staining and Labeling: The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.

Stimulation of renin release from rabbit renal cortex by arachidonic acid and prostaglandin endoperoxides. (1/2940)

The mechanism by which renal prostaglandins stimulate renin secretion in vivo is unknown. In this in vitro study we measured the effects of activation of the prostaglandin (PG) system on renin release from slices of rabbit renal cortex. The PG precursor arachidonic acid (C20:4), a natural PG endoperoxide (PGG2), two stable synthetic PG endoperoxide analogues (EPA I and II), PGE2, PGF2alpha, and two different PG synthesis inhibitors [indomethacin and 5,8,11,14-eicosatetraynoic acid (ETA)] were used to evaluate the possibility of a direct action of the cortical PG system on renin secretion. Renin release increased significantly with time after addition of C20:4, PGG2, EPA I, and EPA II to the incubation medium. Stimulation of renin release was se-related for C20:4 in concentrations of 0.6 to 4.5 X 10(-6) M, for EPA I in concentrations of 0.7 to 2.8 X 10(-6) M, and for EPA II in concentrations of 1.4 to 14.0 X 10(-6) M. Indomethacin (10(-4) M) and ETA (10(-4) M) significantly decreased basal renin release as well as the renin release stimulated by C20:4 and EPA I. PGE2(10(-12) to 10(-6) M) had no effect on renin release, whereas PGF2alpha (10(-12) to 10(-6) M) decreased renin release in a dose-dependent manner. These data raise the possibility of a direct action of the renal cortical PG system on renin secretion. The results further indicate that stimulation of renin release by C20:4 may depend more specifically on the action of PG endoperoxides than on the primary prostaglandins.  (+info)

Isolation and purification of rat mammary tumor peroxidase. (2/2940)

7,12-Dimethylbenz(a)anthracene-induced rat mammary tumors often contain high levels of the enzyme perioxidase, a putative marker of estrogen dependence. This enzyme can be effectively extracted with 0.5 M CaCl2, giving rise to a soluble peroxidase with a molecular weight of about 50,000 as determined by gel filtration. This is the same size as the estrogen-induced peroxidase of rat uterus but smaller than other mammalian peroxidases. Further purification of the rat mammary tumor peroxidase by concanavalin A-Sepharose chromatography and hydrophobic interaction chromatography on phenyl Sepharose provides a 640-fold purification of the enzyme.  (+info)

Cloning of the peroxiredoxin gene family in rats and characterization of the fourth member. (3/2940)

Peroxiredoxin (PRx) exhibits thioredoxin-dependent peroxidase activity and constitutes a family of proteins. Four members of genes from rat tissues were isolated by PCR using degenerated primers based on the sequences which encode a pair of highly conserved Cys-containing domains, and were then cloned to full-length cDNAs. These included two genes which have previously been isolated in rats, PRx I and PRx II, and two rat homologues of PRx III and PRx IV. We showed, for the first time, the simultaneous expression of all four genes in various rat tissues by Northern blotting. Since a discrepancy exists regarding cellular distribution, we further characterized PRx IV by expressing it in COS-1 cells. This clearly demonstrates that PRx IV is a secretory form and functions within the extracellular space.  (+info)

Purification and characterization of a novel peroxidase from Geotrichum candidum dec 1 involved in decolorization of dyes. (4/2940)

A peroxidase (DyP) involved in the decolorization of dyes and produced by the fungus strain Geotrichum candidum Dec 1 was purified. DyP, a glycoprotein, is glycosylated with N-acetylglucosamine and mannose (17%) and has a molecular mass of 60 kDa and an isoelectric point (pI) of 3.8. The absorption spectrum of DyP exhibited a Soret band at 406 nm corresponding to a hemoprotein, and its Na2S2O4-reduced form revealed a peak at 556 nm that indicates the presence of a protoheme as its prosthetic group. Nine of the 21 types of dyes that were decolorized by Dec 1 cells were decolorized by DyP; in particular, anthraquinone dyes were highly decolorized. DyP also oxidized 2,6-dimethoxyphenol and guaiacol but not veratryl alcohol. The optimal temperature for DyP activity was 30 degrees C, and DyP activity was stable even after incubation at 50 degrees C for 11 h.  (+info)

Phospholipid hydroperoxide cysteine peroxidase activity of human serum albumin. (5/2940)

Human serum albumin (HSA) reduced the phospholipid hydroperoxide, 1-palmitoyl-2-(13-hydroperoxy-cis-9, trans-11-octadecadienoyl)-l-3-phosphatidylcholine (PLPC-OOH) to the corresponding hydroxy-derivative with a high apparent affinity (Km=9. 23+/-0.95 microM). Removal of bound lipid during purification increased this activity. At physiological concentration, HSA reduced the phospholipid hydroperoxide in the absence of a cofactor. However, in the presence of a cofactor (reductant), the rate of the reaction was increased. All of the major aminothiols in plasma could act as reductants, the best being the most abundant, cysteine (Km=600+/-80 microM). For every nanomole of PLPC-OOH reduced by HSA, 1.26 nmol of cystine was formed, indicating a reaction stoichiometry of 1 mol PLPC-OOH to 2 mol cysteine. We used chemical modification to determine which amino acid residues on HSA were responsible for the activity. Oxidation of thiol group(s) by N-ethylmaleimide led to a reduction in the rate of activity, whereas reduction of thiols by either dithiothreitol or the angiotensin-converting enzyme inhibitor, captopril, increased the activity. Both N-ethylmaleimide-modified HSA and dithiothreitol-treated HSA exhibited increased apparent affinities for PLPC-OOH. For a range of preparations of albumin with different modifications, the activity on PLPC-OOH was dependent on the amount of free thiol groups on the albumin (correlation coefficient=0.91). Patients with lowered albumin concentrations after septic shock showed lowered total plasma thiol concentrations and decreased phospholipid hydroperoxide cysteine peroxidase (PHCPx) activities. These results therefore show for the first time that HSA exhibits PHCPx activity, and that the majority of the activity depends on the presence of reduced thiol group(s) on the albumin.  (+info)

Use of site-directed mutagenesis to probe the structure, function and isoniazid activation of the catalase/peroxidase, KatG, from Mycobacterium tuberculosis. (6/2940)

A series of mutants bearing single amino acid substitutions often encountered in the catalase/peroxidase, KatG, from isoniazid-resistant isolates of Mycobacterium tuberculosis has been produced by site-directed mutagenesis. The resultant enzymes were overexpressed, purified and characterized. Replacing Cys-20 by Ser abolished disulphide-bridge formation, but did not affect either dimerization of the enzyme or catalysis. The substitution of Thr-275, which is probably involved in electron transfer from the haem, by proline resulted in a highly unstable enzyme with insignificant enzyme activities. The most commonly occurring substitution in drug-resistant clinical isolates is the replacement of Ser-315 by Thr; this lowered catalase and peroxidase activities by 50% and caused a significant decrease in the KatG-mediated inhibition of the activity of the NADH-dependent enoyl-[acyl-carrier protein] reductase, InhA, in vitro. The ability of this enzyme to produce free radicals from isoniazid was severely impaired, as judged by its loss of NitroBlue Tetrazolium reduction activity. Replacement of Leu-587 by Pro resulted in marked instability of KatG, indicating that the C-terminal domain is also important for structural and functional integrity.  (+info)

Direct interaction of lignin and lignin peroxidase from Phanerochaete chrysosporium. (7/2940)

Binding properties of lignin peroxidase (LiP) from the basidiomycete Phanerochaete chrysosporium against a synthetic lignin (dehydrogenated polymerizate, DHP) were studied with a resonant mirror biosensor. Among several ligninolytic enzymes, only LiP specifically binds to DHP. Kinetic analysis revealed that the binding was reversible, and that the dissociation equilibrium constant was 330 microM. The LiP-DHP interaction was controlled by the ionization group with a pKa of 5.3, strongly suggesting that a specific amino acid residue plays a role in lignin binding. A one-electron transfer from DHP to oxidized intermediates LiP compounds I and II (LiPI and LiPII) was characterized by using a stopped-flow technique, showing that binding interactions of DHP with LiPI and LiPII led to saturation kinetics. The dissociation equilibrium constants for LiPI-DHP and LiPII-DHP interactions were calculated to be 350 and 250 microM, and the first-order rate constants for electron transfer from DHP to LiPI and to LiPII were calculated to be 46 and 16 s-1, respectively. These kinetic and spectral studies strongly suggest that LiP is capable of oxidizing lignin directly at the protein surface by a long-range electron transfer process. A close look at the crystal structure suggested that LiP possesses His-239 as a possible lignin-binding site on the surface, which is linked to Asp-238. This Asp residue is hydrogen-bonded to the proximal His-176. This His-Asp...proximal-His motif would be a possible electron transfer route to oxidize polymeric lignin.  (+info)

Eosinophil peroxidase increases membrane permeability in mammalian urinary bladder epithelium. (8/2940)

Eosinophil peroxidase (EPO), a cationic protein found in eosinophils, has been reported to be cytotoxic independent of its peroxidase activity. This study investigated with electrophysiological methods whether EPO is toxic to mammalian urinary bladder epithelium. Results indicate that EPO, when added to the mucosal solution, increases apical membrane conductance of urinary bladder epithelium only when the apical membrane potential is cell interior negative. The EPO-induced conductance was concentration dependent, with a maximum conductance of 411 microseconds/cm2 and a Michaelis-Menten constant of 113 nM. The EPO-induced conductance was nonselective for K+ and Cl-. The conductance was partially reversed using voltage but not by removal of EPO from the bulk solution. Mucosal Ca2+ reversed the EPO-induced conductance by a mechanism involving reversible block of the conductance. Prolonged exposure (up to 1 h) to EPO was toxic to the urinary bladder epithelium, as indicated by an irreversible increase in transepithelial conductance. These results suggest that EPO is indeed toxic to urinary bladder epithelium via a mechanism that involves an increase in membrane permeability.  (+info)

In order to understand the mechanism of molecular interactions at the active site of Tryparedoxin Peroxidase (Try P), homology modeling and docking studies were performed. We generated a Three-Dimensional (3D) model of target protein based on the Crystal structure of Leishmania Major Try PI (PDB ID: 3TUE) using modeler software. Docking analysis was carried out to study the effects of methotrexate on Tryparedoxin Peroxidase (Try P). Inhibition of the Tryparedoxin peroxidase interaction has become a new therapeutic strategy in treating leishmaniasis. Docking analysis was carried out to study the effects of methotrexate on Tryparedoxin Peroxidase (TryP). Tryparedoxin peroxidase of Trypanosomatidae family functions as antioxidant through their peroxidase and peroxynitrite reductase activities. The theoretical docking study, conducted on a sample previously reported for anti-cancer properties of Methotrexate at the binding site of 3D models of Tryparedoxin Peroxidase of Leishmania braziliensis (L. ...
TY - JOUR. T1 - Expression of a moderately anionic peroxidase is induced by aluminum treatment in tobacco cells. T2 - Possible involvement of peroxidase isozymes in aluminum ion stress. AU - Ezaki, Bunichi. AU - Tsugita, Shinobu. AU - Matsumoto, Hideaki. PY - 1996/1. Y1 - 1996/1. N2 - To clarify the mechanism of aluminum (Al) toxicity and Al tolerance, we isolated a new clone (pAL201) from a tobacco cDNA library. Northern blot hybridization analysis indicated that the expression of pAL201 is induced by Al treatment and phosphate (Pi) starvation. The complete cDNA sequence suggested that this clone encodes a moderately anionic peroxidase (EC 1.11.1.7). Analysis by isoelectric focussing indicated that a moderately anionic peroxidase (approximately pI 6.7) and two cationic peroxidases (pI 9.2 and 9.7) in the soluble fraction are activated by Al treatment and Pi starvation, while two moderately anionic isozymes are repressed by these stresses. We suppose that Al ion stress can control the activity ...
Heme peroxidases are widely distributed in biological systems and are involved in a wide range of processes essential for life. This book provides a comprehensive single source of information on the various aspects of heme peroxidase structure, function and mechanism of action. Chapters written and edited by worldwide experts span a range of heme peroxidases from plants, yeast, bacteria and mammals. Discussed functions of peroxidases range from cell wall synthesis, synthesis of prostaglandins, role in drug suppression of tuberculosis, and antibacterial activity. Included is a discussion of peroxidases that also act as catalases and oxygenases. Heme Peroxidases serves as an essential text for those working in industry and academia in biochemistry and metallobiology.
Minute Peroxidase Suppressor is designed to inhibit endogenous peroxidase activity commonly encountered in immunohistochemistry (IHC) procedures. Inhibiting endogenous peroxidase activity is essential for avoiding false positive and reduce the background of IHC. Many chemical agents such as H2O2 and sodium aside have been used for this purpose but the inhibition is usually incomplete. Minute TM HRP Suppressor is a mixture of several potent HRP inhibitors and the result is a complete inhibitionof endogenous peroxidase activity. The suppressor can be used in regular IHC as well as HRP-mediated super sensitive procedures such as tyramide signal amplification (TSA). Major Features: Very stable at RT, ready to use and irreversible inhibition of endogenous peroxidase activity. Significantly reduce the background of sensitive assays using HRP. 1. After cell or tissue samples are prepared on slide, perform antigen retrieval step if necessary. 2. Block non-specific sites in the samples with normal serum ...
Infinite Enzymes Manganese Peroxidase Enzyme (MnP) is a recombinant peroxidase from Phanerochaete chrysosporium produced in corn seed. Request Quote now!
Alterations in Soluble Class III Peroxidases of Maize Shoots by Flooding Stress. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Thiol peroxidases are critical enzymes in the redox control of cellular processes that function by reducing low levels of hydroperoxides and regulating redox signaling. These proteins were also shown to regulate genome stability, but how their dysfunction affects the actual mutations in the genome is not known. Saccharomyces cerevisiae has eight thiol peroxidases of glutathione peroxidase and peroxiredoxin families, and the mutant lacking all these genes (Δ8) is viable. In this study, we employed two independent Δ8 isolates to analyze the genome-wide mutation spectrum that results from deficiency in these enzymes. Deletion of these genes was accompanied by a dramatic increase in point mutations, many of which clustered in close proximity and scattered throughout the genome, suggesting strong mutational bias. We further subjected multiple lines of wild-type and Δ8 cells to long-term mutation accumulation, followed by genome sequencing and phenotypic characterization. Δ8 lines showed a ...
White-rot fungi (WRF) are capable of degrading complex organic compounds such as lignin, and the enzymes that enable these processes can be used for the detoxification of recalcitrant organopollutants. The aim of this study is to evaluate a system based on the use of an in vitro ligninolytic enzyme for the detoxification of recalcitrant dye pollutants. The dyes selected for investigation were the anionic and cationic commercial azo dyes, basic blue 41 (BB41), acid black 1 (AB1), and reactive black 5 (RB5). A supernatant, cell-free culture of WRF with manganese peroxidase activity was used to investigate its degradative capacity under various conditions, and concentrations of cofactors, H2O2 and Mn2+. The assays were carried out using a 22 experimental designs whose variables were concentration of Mn2+ (33 and 1,000 μM) and semicontinuous dosage of the H2O2 (0.02 and 0.10 μmol) added at a frequency of 0.2 min−1. The response variables analyzed were the efficiency and the initial rate of the ...
산화적 스트레스(Oxidative Stress)는 Reactive Oxygen Species (ROS)와 antioxidant (항산화제)간의 불균형에 의해 발생되며, ROS의 과도한 축적은 DNA, 단백질 및 지질 세포막의 손상과 같은 세포적 손상을 초래합니다.. 산화적 스트레스의 조건에서 발생되는 Hydrogen Peroxide (H2O2)와 같은 과산화물(peroxide)은 ROS의 대표적인 산물 중 하나로 진핵세포에서는 유독하며, 높은 농도에서는 DNA, 지질 단백질의 산화를 일으켜 돌연변이 유발이나 세포사멸을 일으키기도 합니다.. peroxide에 의한 세포적 손상은 노화, 천식, 관절염, 당뇨병, 심혈관 질환, 신경성 퇴행성 질환 등 여러 질환의 발병과도 관계가 있습니다. EZ-Hydrogen Peroxide/Peroxidase assay kit는 Oxi-Probe를 사용하여 Hydrogen Peroxide (H2O2) 또는 Peroxidase 활성을 측정할 수 있는 제품으로 간단한 실험 방법과 민감도를 가지는 제품입니다. 본 Kit는 ...
Title: Recents Patents in the Use of Peroxidases. VOLUME: 3 ISSUE: 2. Author(s):Berenize Alvarado and Eduardo Torres. Affiliation:Benemerita Universidad Autonoma de Puebla, Centro de Quimica-ICUAP. Ciudad Universitaria, Puebla, 72570. Mexico.. Keywords:Biosensor, enzymatic oxidation, peroxidases, review. Abstract: Peroxidases are hemoenzymes with a wide range of applications, from fine chemical synthesis to environmental biocatalysis. These outstanding biocatalysts are able to catalyze reactions such as heteroatom oxidation (Nand S-oxidation), epoxidation, hydroxylation, and the oxidation of alcohols and indole, often giving high yields and enantiomeric excess values. This makes these biocatalysts very useful for application to several biotechnological processes. In this paper, recent advances and patents surrounding the use of peroxidases are reviewed, covering different aspects related to the applications of peroxidases and the modifications carried out to improve their functionality as ...
casSAR Dugability of Q73WB6 | MAP_2744c | Catalase-related peroxidase - Also known as CRPE_MYCPA, MAP_2744c. Has an organic peroxide-dependent peroxidase activity. Exhibits strong peroxidase activity using organic hydroperoxides as cosubstrates, weak peroxidase activity using hydrogen peroxide and negligible catalase activity. May have a role in elimination of reactive oxygen species, in particular by deactivating hydroperoxides. Monomer.
There is another endogenous peroxidase blocking method the blocks peroxidases and pseudoperoxidases. It can be used for minimally fixed frozen sections and in your case, paraffin sections. The is very thorough and gentle. It is called Glucose Oxidase blocking method. We love it for frozens as it does not chew sections off the slide. We have not tried it on paraffin but I have it by private communication it will work with paraffin sections/NBF fixed. I have seen it reported as 30 min in the working solution but original publication used it for 60 min. I will be happy to attach the method to you, not via Histonet. At 09:21 PM 6/23/2004 -0400, you wrote: >Todd, > Youre right. Sorry I havent responded to everyones suggestions >on my posting on blocking endogenous peroxidase in paraffin >sections. I think those who suggested that biotin might be the >problem have a point, however when I ran control sections with >just buffer, blocking (10% goat serum), and DAB, I still got >brown, even though ...
Fullscreen (supported by IE11, latest versions of Firefox, Chrome, Safari (not including iOS Safari), Edge, Chrome for Android, Samsung Internet) ...
Hamster mast cells have been found to give strong peroxidatic reactions at pH 5, 7.5 and 10 when sections of skeletal muscle are incubated for 2.5 h in the dark at room temperature on semipermeable...
Science and Education Publishing, publisher of open access journals in the scientific, technical and medical fields. Read full text articles or submit your research for publishing.
Enzyme Explorer - Peroxidase Enzymes products. Sigma-Aldrichs peroxidase product is recognized around the world as the industry standard for diagnostic manufacturing and laboratory scale research applications.
DAB (3, 3 -diaminobenzidine) HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications. DAB chromogen is effective as a single label or as a second color for multiple antigen labeling.
Vector|sup|®|/sup| NovaRED™ and ImmPACT™ NovaRED™ HRP substrates produce red reaction products. Unlike AEC, sections stained with either substrate should be dehydrated, cleared, and permanently mounted.
1. Thaw plates before opening bags to prevent condensation in the wells. 2. Add 200ul Blocking Buffer to each well and incubate 1 hour @ room temperature (RT). 3. Wash 3x w/ PBST. Aspirate and blot dry. 4. Prepare standards and samples in PBS. 5. Add 100ul of dilutions to wells. 6. Incubate plate 2-3h @ RT. 7. Incubate o/n @ 4C. 8. Wash 3x w/ PBST. Aspirate and blot dry. 9. Dilute biotinylated mAB211 in PBST + 0.5% Bovine Serum Albumin (BSA). 10. Add 100ul diluted Ab to each well. Incubate 2h @ RT. 11. Wash 3x w/ PBST. Aspirate and blot dry. 12. Prepare HRP-Streptavidin...... 13. Add 100ul diluted HRP-Streptavidin to each well. Incubate 2h @ RT. 14. Wash 3x w/ PBST. Aspirate and blot dry. 15. Prepare Peroxidase Substrate immediately before use. 16. Add 100ul Peroxidase Substrate to each well and incubate 10min in the dark @ RT. 17. Add 100ul Stopper Solution to wells and read plate at 490nm. ...
peroxidase, putative; FUNCTIONS IN: electron carrier activity, peroxidase activity, heme binding; INVOLVED IN: response to oxidative stress; LOCATED IN: endomembrane system; EXPRESSED IN: 10 plant structures; EXPRESSED DURING: 6 growth stages; CONTAINS InterPro DOMAIN/s: Haem peroxidase (InterPro:IPR010255), Plant peroxidase (InterPro:IPR000823), Haem peroxidase, plant/fungal/bacterial (InterPro:IPR002016); BEST Arabidopsis thaliana protein match is: peroxidase, putative (TAIR:AT5G58400.1); Has 2891 Blast hits to 2875 proteins in 212 species: Archae - 0; Bacteria - 4; Metazoa - 2; Fungi - 66; Plants - 2777; Viruses - 0; Other Eukaryotes - 42 (source: NCBI BLink ...
Streptococcus pneumoniae is a facultative anaerobic pathogen. Although it maintains fermentative metabolism, during aerobic growth pneumococci produce high levels of H2O2, which can have adverse effects on cell viability and DNA, and influence pneumococcal interaction with its host. The pneumococcus is unusual in its dealing with toxic reactive oxygen species in that it neither has catalase nor the global regulators of peroxide stress resistance. Previously, we identified pneumococcal thiol peroxidase (TpxD) as the key enzyme for enzymatic removal of H2O2, and showed that TpxD synthesis is up-regulated upon exposure to H2O2. This study aimed to reveal the mechanism controlling TpxD expression under H2O2 stress. We hypothesize that H2O2 activates a transcription factor which in turn up-regulates tpxD expression. Microarray analysis revealed a pneumococcal global transcriptional response to H2O2. Mutation of tpxD abolished H2O2-mediated response to high H2O2 levels, signifying the need for an active TpxD
Peroxidase Conjugated Affinity Purified Anti-SHEEP IgG F(ab)2 (RABBIT), Peroxidase Conjugated Affinity Purified anti-Sheep IgG F(ab )2 [Rabbit]; N/A Peroxidase Conjugated Affinity Purified Anti-SHEEP IgG F(ab)2 (RABBIT)IGHG1
cis-regulatory elements of the peroxidase gene in Arabidopsis thaliana involved in root-specific expression and responsiveness to high-salt stress.: The pattern
The Determination of Peroxidase in Amniotic Fluid.: Analyzing total peroxidase activity in amniotic fluid is extremely simple, requiring only 1 1/2 minutes of i
Within biological systems iron is a transition metal that allows access to the benefits of molecular oxygen as an oxidant. However, with these benefits come grave consequences if the reactions are not strictly controlled. The most prominent strategy of control and specialization is the protein environment that surrounds iron. Within iron containing proteins, specifically heme proteins, there are four basic levels of structure that impact the irons function: cofactor structure, protein-supplied ligands, non-ligand active site environment, and protein features that are distant from the active site. This last level remains poorly understood due to a lack of good models to pursue such studies. Catalase-peroxidases are unique heme proteins because they catalyze peroxide decomposition by two separate mechanisms, catalase and peroxidase, using the same active site. However, were it not for three structural features distant from the active site, catalase-peroxidases would be practically superimposable ...
1NHQ: Crystallographic analyses of NADH peroxidase Cys42Ala and Cys42Ser mutants: active site structures, mechanistic implications, and an unusual environment of Arg 303.
Purified Native R. communis Lectin Toxin RCA60, Peroxidase labeled from Creative Biomart. Native R. communis Lectin Toxin RCA60, Peroxidase labeled can be used for research.
1NHP: Crystallographic analyses of NADH peroxidase Cys42Ala and Cys42Ser mutants: active site structures, mechanistic implications, and an unusual environment of Arg 303.
is this on human tissues? abizar www.innogenex.com 1-877-igx-info -----Original Message----- From: [email protected] [mailto:[email protected]] Sent: Wednesday, May 03, 2000 3:22 PM To: [email protected] Subject: controls for rabbit polyclonals Plea for help, I am unable to get clean control slides for any rabbit polyclonal antibodies on either paraffin or frozen sections. There is always background (often very dark) on the collagen, smooth muscle (especially prostate) etc. This happens with commercial antibodies (B2microglobulin or vonWillebrand), in house Abs, as well as with rabbit IgG. I use casein or superblock. Avidin-Biotin block and exhaust endogenous peroxidase. I use the ABC kit with DAB. There is no problem if I omit the primary antibody. Can anybody offer any insights? Thank you, Bonnie ...
Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Sodium atom in PDB 2b2r: Crystal Structure Of An Oxoferryl Species of Catalase- Peroxidase Katg At PH5.6
peroxidase: Any of a group of enzymes that occur especially in plant cells and catalyze the oxidation of a substance by a peroxide.
anti-Glutathione Peroxidase 7, Polyclonal, Novus Biologicals 0.1mL; Unlabeled Life Sciences:Antibodies:Primary Antibodies:Immunoprecipitation (IP)
Histofine Simple Stain MAX Peroxidase (Human Tissue, Mouse primary): 414131F by As One International, Inc. at Labscoop.com - Read reviews, citations, datasheets, protocols & more.
To promote science and increase scientific awareness on recent developments around the world. Provide tools for all science loving people to enhance, educate and share their Science knowledge and skills and benefit from interaction with each other
Click on a taxon to see the following taxons, if there are any.. By placing the mouse over a node a tooltip will appear. This tootip contains the taxonomic path from "Cellular organisms" to the node and, in the case of a species, the number of peroxidases in the orthogroup, the total number of peroxidases for that species in the orthogroups class and where possible the names of those peroxidases. The common ancestor for the orthogroup is written in bold in the tooltip. Return to orthogroup ...
The orthogroup DiHCcP001 is composed of 84 sequences in 68 species. Click here to see a graphical representation of the species in this orthogroup.. ...
C.E.T. Chews For Dogs Lg 30 Ct. 30 count bag of beefhide strips that average 2 x 6 inches. Treated with the C.E.T. Dual-Enzyme System which enhances and augments a naturally-occurring oral defense mechanism, the Salivary Peroxidase System.
Dear Experts, I would like to label an antibody with peroxidase by the periodate method. Which HRP grade / isoforms / purity should I use? Is there a practical advantage (or even disadvantage) of stabilized peroxidase, where the amino groups are chemically protected and therefore oligomerization of the HRP would be prevented? The AB will be used in westerns and Elisa. Many thanks! Wo ...
Your basket is currently empty. i ,p>When browsing through different UniProt proteins, you can use the basket to save them, so that you can back to find or analyse them later.,p>,a href=/help/basket target=_top>More...,/a>,/p> ...
A cDNA for thylakoid-bound ascorbate peroxidase of pumpkin was cloned and characterized. Thylakoid-bound ascorbate peroxidase had a high similarity to cytosolic ascorbate peroxidases, and the precursor contained a transit peptide to chloroplasts at its ammo-terminus and a putative membrane-spanning region at its carboxy-terminus.. ...
The ionic strength dependence of the reduction of cytochrome c peroxidase compound I by horse cytochrome c has been studied, using stopped-flow technigue, in pH 7.5, potassium phosphate/nitrate buffer. The temperature was set at 25 ± 1° C. The wavelength was monitored at 4 32 nm, an isobestic point for ferri-/ferrocytochrome c, so only the absorbance change of the reduction of cytochrome c peroxidase compound I to compound II can be observed. The observed rate constant, kobs, as a function of the concentration of ferrocytochrome c shows a non-linear increase with increasing ionic strength. A two-parameter eguation is needed to fit the data at low ionic strength, 20 mM to 40 mM, while a three-parameter equation is needed at high ionic strength, 65 mM and above. The maximum rates of these reductions also show two different types of ionic strength dependence. At 20 mM to 40 mM ionic strength, the maximum rate of reduction decreases slightly, within experimental error, with increasing ionic ...
We previously demonstrated that manganese peroxidase from the white-rot fungus Phanerochaete chrysosporium was very susceptible to thermal inactivation due to the loss of calcium from the enzyme [Sutherland & Aust (1996) Arch. Biochem. Biophys. 332, 128-134]. The structural changes that occur during thermal inactivation and the release of calcium from manganese peroxidase have now been characterized. Thermal inactivation caused distinct alterations in the heme environment and slight changes in the overall protein structure, both of which were reversed upon reactivation of the enzyme with calcium. The absorption spectrum of inactivated manganese peroxidase was similar to that of low-spin ferric heme proteins, indicating that a sixth ligand had bound to the distal side of the heme iron. Consistent with disruption of the distal heme environment, thermally inactivated manganese peroxidase did not react with hydrogen peroxide to form compound I. The inactive enzyme exhibited a pH-dependent absorption
Species of the genus Pleurotus are among the most efficient natural species in lignin degradation belonging to the subclass of ligninolytic organisms that produce laccase (Lac), Mn-dependent peroxidase (MnP), versatile peroxidase (VP), and the H2O2-generating enzyme aryl-alcohol oxidase, but not lignin peroxidases. Production of Lac and oxidation of 2,6-dimethoxyphenol (DMP) in the presence and absence of Mn2+ were detected both in submerged fermentation (SF) of dry ground mandarine peels and in solid-state fermentation (SSF) of grapevine sawdust in all investigated Pleurotus species and strains. Evidence of cultivation methods having a distinct influence on the level of enzyme activities has been demonstrated. Most of the species and strains had higher Lac activity under SSF conditions than under SF conditions. DMP oxidation in the presence and absence of Mn2+ was detected in all investigated species and strains, but was lower under SF conditions than under SSF conditions for most of ...them. ...
Additional file 19: of Genome-wide and evolutionary analysis of the class III peroxidase gene family in wheat and Aegilops tauschii reveals that some members are involved in stress responses
Author(s): Smith, Martyn T.; Yager, J; Steinmetz, K; Eastmond, D | Abstract: The metabolism of two of benzenes phenolic metabolites, phenol and hydroquinone, by peroxidase enzymes has been studied in detail. Studies employing horseradish peroxidase and human myeloperoxidase have shown that in the presence of hydrogen peroxide phenol is converted to 4,4-diphenoquinone and other covalent binding metabolites, whereas hydroquinone is converted solely to 1,4-benzoquinone. Surprisingly, phenol stimulates the latter conversion rather than inhibiting it, an effect that may play a role in the in vivo myelotoxicity of benzene. Indeed, repeated coadministration of phenol and hydroquinone to B6C3F1 mice results in a dramatic and significant decrease in bone marrow cellularity similar to that observed following benzene exposure. A mechanism of benzene-induced myelotoxicity is therefore proposed in which the accumulation and interaction of phenol and hydroquinone in the bone marrow and the peroxidase-dependent
Poole, L.B., and Ellis, H.R. (1996) Flavin-dependent alkyl hydroperoxide reductase from Salmonella typhimurium. 1. Purification and enzymatic activities of overexpressed AhpF and AhpC proteins. Biochemistry 35, 56-64. PDF of article Poole, L.B. (1996) Flavin-dependent alkyl hydroperoxide reductase from Salmonella typhimurium. 2. Cystine disulfides involved in catalysis of peroxide reduction. Biochemistry 35, 65-75. PDF of article Poole, L.B., Chae, H.Z., Flores, B.M., Reed, S.L., Rhee, S.G., and Torian, B.E. (1997) Peroxidase activity of a TSA-like antioxidant protein from a pathogenic amoeba. Free Radical Biol. Med. 23, 955-959. PDF of article Li Calzi, M., and Poole, L.B. (1997) Requirement for the two AhpF cystine disulfide centers in catalysis of peroxide reduction by alkyl hydroperoxide reductase. Biochemistry 36, 13357-13364. PDF of article Ellis, H. R., and Poole, L.B. (1997) Roles for the two cysteine residues of AhpC in catalysis of peroxide reduction by alkyl hydroperoxide reductase ...
thyroid peroxidase - MedHelps thyroid peroxidase Center for Information, Symptoms, Resources, Treatments and Tools for thyroid peroxidase. Find thyroid peroxidase information, treatments for thyroid peroxidase and thyroid peroxidase symptoms.
Lactoperoxidase is a peroxidase enzyme secreted from mammary, salivary, and other mucosal glands that functions as a natural antibacterial agent. Lactoperoxidase is a member of the heme peroxidase family of enzymes. In humans, lactoperoxidase is encoded by the LPO gene. Lactoperoxidase catalyzes the oxidation of a number of inorganic and organic substrates by hydrogen peroxide. These substrates include bromide and iodide and therefore lactoperoxidase can be categorised as a haloperoxidase. Another important substrate is thiocyanate. The oxidized products produced through the action of this enzyme have potent bactericidal activities. Lactoperoxidase together with its inorganic ion substrates, hydrogen peroxide, and oxidized products is known as the lactoperoxidase system. The lactoperoxidase system plays an important role in the innate immune system by killing bacteria in milk and mucosal (linings of mostly endodermal origin, covered in epithelium, which are involved in absorption and secretion) ...
en] The cloning and sequencing of the rbpa gene coding for a versatile peroxidase from a novel Bjerkandera strain is hereby reported. The 1777 bp isolated fragment contained a 1698 bp peroxidase-encoding gene, interrupted by 11 introns. The 367 amino acid-deduced sequence includes a 27 amino acid-signal peptide. The molecular model, built via homology modelling with crystal structures of four fungal peroxidases, highlighted the amino acid residues putatively involved in manganese binding and aromatic substrate oxidation. The potential heme pocket residues (R44, F47, H48, E79, N85, H177, F194 and D239) include both distal and proximal histidines (H48 and H177). RBP possesses potential calcium-binding residues (D49, G67, D69, S71, S178, D195, T197, I200 and D202) and eight cysteine residues (C3, C15, C16, C35, C121, C250, C286, C316). In addition, RIBP includes residues involved in substrate oxidation: three acidic residues (E37, E41 and D183)-putatively involved in manganese binding and H83 and ...
Peroxidase. References[edit]. *^ a b PDB: 1VAR​; Borgstahl GE, Parge HE, Hickey MJ, Johnson MJ, Boissinot M, Hallewell RA, ...
Banerjee, R. K.; Datta, A. G. (1986). "Salivary peroxidases". Mol Cell Biochem. 70 (1): 21-29. doi:10.1007/bf00233801. PMID ... Banerjee, R. K.; Bose, A. K.; Chakraborty, T. K.; De, S. K.; Datta, A. G. (1985). "Peroxidase-catalysed iodotyrosine formation ... lysozyme and peroxidase.[15] It has not been shown that human licking of wounds disinfects them, but licking is likely to help ...
Peroxidases enzyme. *Ureas an organic compounds. *Chitinase enzyme. References[edit]. *^ Brown, Thomas A. "Rapid Review ...
Glutathione peroxidase activity of inorganic selenium and seleno-DL-cysteine. Experientia 31: 769-770, 1975 ... Electrophoretic polymorphism of glutathione peroxidase. Ann Hum Genet 38: 163-169, 1974 ...
2. Prostaglandin G/H synthase 1 ... General function: Involved in peroxidase activity. ...
There are at least four different glutathione peroxidase isozymes in animals.[154] Glutathione peroxidase 1 is the most ... Peroxidases. catalase. H. 2. O. Water. {\displaystyle {\ce {{\underset {Oxygen}{O2}}-,{\underset {Superoxide}{*O2^{-}}}-,[{\ce ... Surprisingly, glutathione peroxidase 1 is dispensable, as mice lacking this enzyme have normal lifespans,[155] but they are ... Peroxiredoxins are peroxidases that catalyze the reduction of hydrogen peroxide, organic hydroperoxides, as well as ...
Existem pelo menos quatro isozimas glutationa peroxidases diferentes nos animais.[131] A glutationa peroxidase 1 é a mais ... Por sua vez, a glutationa peroxidase 3 é mais activa contra os hiperóxidos lípidos. Surpreendentemente, a glutationa peroxidase ... O sistema da glutationa contém glutationa, glutationa redutase, glutationa peroxidases e glutationa S-transferase.[74] Este ... Brigelius-Flohé R (1999). «Tissue-specific functions of individual glutathione peroxidases». Free Radic Biol Med. 27 (9-10): ...
... catalyzed by the presence of a peroxidase (such as horseradish peroxidase). The hydrogen peroxide is itself produced by an ... CS1 maint: Multiple names: authors list (link) Carlos G. Dosoretz; Gary Ward (2006). "Peroxidases". In Ashok Pandey; Colin Webb ...
White WE, Pruitt KM, Mansson-Rahemtulla B (February 1983). "Peroxidase-Thiocyanate-Peroxide Antibacterial System Does Not ... Hypothiocyanite is formed by peroxidase catalysis of hydrogen peroxide and thiocyanate: H2O2 + SCN− → OSCN− + H2O ... "Active site structure and catalytic mechanisms of human peroxidases". Arch. Biochem. Biophys. 445 (2): 199-213. doi:10.1016/j. ... respiratory syncytial virus and echovirus type 11 by peroxidase-generated hypothiocyanite. Antiviral Res. 1995 Mar;26(2):161-71 ...
In the colloid, iodide (I−) is oxidized to iodine (I0) by an enzyme called thyroid peroxidase. - Iodine (I0) is very reactive ... Antibodies against thyroid peroxidase can be found on testing. The inflammation usually resolves without treatment, although ... and the iodine is attached to the active tyrosine units in thyroglobulin by the enzyme thyroid peroxidase. This forms the ...
... l is one important characteristic of animal peroxidases; plant peroxidases incorporate heme B. Lactoperoxidase and ... In peroxidase reactions, the porphyrin molecule also serves as an electron source. In the transportation or detection of ... Heme l is the derivative of heme B which is covalently attached to the protein of lactoperoxidase, eosinophil peroxidase, and ... Thyroid peroxidase is the enzyme catalyzing the biosynthesis of the important thyroid hormones. Because lactoperoxidase ...
Glutathione peroxidase 3 (GPx-3), also known as plasma glutathione peroxidase (GPx-P) or extracellular glutathione peroxidase ... 2008). "Glutathione peroxidase 3 is a candidate mechanism of anticancer drug resistance of ovarian clear cell adenocarcinoma". ... 2009). "Glutathione peroxidase 3 gene polymorphisms and risk of differentiated thyroid cancer". Surgery. 145 (5): 508-13. doi: ... 2009). "Glutathione peroxidase 3 mediates the antioxidant effect of peroxisome proliferator-activated receptor gamma in human ...
"Entrez Gene: glutathione peroxidase 6 (olfactory)".. *^ Kryukov GV, Castellano S, Novoselov SV, Lobanov AV, Zehtab O, Guigó R, ... Glutathione peroxidase 6 (GPx-6) is an enzyme that in humans is encoded by the GPX6 gene.[5][6] ... This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It ... 2001). "Human immunodeficiency virus type 1 Tat protein impairs selenoglutathione peroxidase expression and activity by a ...
55 µg/day recommendation is based on full expression of plasma glutathione peroxidase. Selenoprotein P is a better indicator of ... Because of selenium's role in certain peroxidases (converting hydroperoxides to alcohols) and because of the antioxidant role ... Kraus, RJ; Prohaska, JR; Ganther, HE (1980). "Oxidized forms of ovine erythrocyte glutathione peroxidase. Cyanide inhibition of ... localization of peroxidases and vitamin E differs, partly because of the fat-solubility of vitamin E.) Some studies have ...
... the presence of catalase or other peroxidases in these organisms can increase tolerance in the presence of lower concentrations ...
peroxidase activity. • prostaglandin-endoperoxide synthase activity. Cellular component. • cytoplasm. • endoplasmic reticulum ... A hydroperoxide oxidizes the heme to a ferryl-oxo derivative that either is reduced in the first step of the peroxidase cycle ... Each monomer of the enzyme has a peroxidase and a PTGS (COX) active site. The PTGS (COX) enzymes catalyze the conversion of ... Second, PGG2 is reduced to PGH2 in the peroxidase active site. The synthesized PGH2 is converted to prostaglandins (PGD2, PGE2 ...
and peroxidases - are enzymes. They often activate O2 for oxidation or hydroxylation. Hemeproteins also enable electron ...
Peroxidases and photosynthetic pigments in susceptible potato infected with potato virus YNTN. Plant Physiol Bioch 39: 891-898 ... lower chlorophyll levels and differential activity of soluble and ionically-bound peroxidases were detected. At later stages of ...
It is commonly used as a substrate with hydrogen peroxide for a peroxidase enzyme (such as horseradish peroxidase) or alone ... Its use allows the reaction kinetics of peroxidases themselves to be followed. In this way it also can be used to indirectly ...
... can also catalyze several redox reactions such as hydroxylation and aromatic oxidation, and shows peroxidase ... During the early phase of apoptosis, mitochondrial ROS production is stimulated, and cardiolipin is oxidized by a peroxidase ... "Sedimentation equilibrium studies on the interaction between cytochrome c and cytochrome c peroxidase". Archives of ...
Kauppila A, Korpela H, Mäkilä UM, Yrjänheikki E (January 1987). "Low serum selenium concentration and glutathione peroxidase ...
Hiner A, Raven E, Thorneley R, García-Cánovas F, Rodríguez-López J «Mechanisms of compound I formation in heme peroxidases». J ... Les peroxiredoxiness són peroxidases que catalitzen la reducció de peròxid d'hidrogen, hidroperòxid orgànic i peroxinitrit.[73] ... Brigelius-Flohé R «Tissue-specific functions of individual glutathione peroxidases». Free Radic Biol Med, 27, 9-10, 1999, pàg. ... Ho Y, Magnenat J, Bronson R, Cao J, Gargano M, Sugawara M, Funk C «Mice deficient in cellular glutathione peroxidase develop ...
"PeroxiBase - The peroxidase database". Swiss Institute of Bioinformatics. Archived from the original on 2008-10-13. Retrieved ... Enzyme kinetics Peroxidases Superoxide dismutase GRCh38: Ensembl release 89: ENSG00000121691 - Ensembl, May 2017 GRCm38: ... Maehly AC, Chance B (1954). "The assay of catalases and peroxidases". Methods of Biochemical Analysis. Methods of Biochemical ... contains catalases and peroxidases. To activate the noxious spray, the beetle mixes the contents of the two compartments, ...
... produces chitinase and peroxidase. Wang, W; Mai-Gisondi, G; Stogios, PJ; Kaur, A; Xu, X; Cui, H; ... Iqbal, M.; Mercer, D. K.; Miller, P. G. G.; McCarthy, A. J. (1 June 1994). "Thermostable extracellular peroxidases from ...
Rhee SG, Kang SW, Chang TS, Jeong W, Kim K (July 2001). "Peroxiredoxin, a novel family of peroxidases". IUBMB Life. 52 (1-2): ... Catalase Oxidative stress Peroxidase Peroxiredoxin classification index Reactive oxygen species Superoxide dismutase Rhee S, ... Other names include thiol specific antioxidant (TSA) and thioredoxin peroxidase (TPx). Mammals express six peroxiredoxins: 1- ... Poole LB (January 2005). "Bacterial defenses against oxidants: mechanistic features of cysteine-based peroxidases and their ...
Thylakoid-bound ascorbate peroxidase had a high similarity to cytosolic ascorbate peroxidases, and the precursor contained a ... Thylakoid-bound ascorbate peroxidase had a high similarity to cytosolic ascorbate peroxidases, and the precursor contained a ... Keywords: Ascorbate peroxidase (EC 1.11.1.11); Chloroplasts; Oxidative stress; Putative membrane-spanning domain; Thylakoid- ... A cDNA for thylakoid-bound ascorbate peroxidase of pumpkin was cloned and characterized. ...
... to a pure oxygen atmosphere under conditions of nutrient starvation has been widely adopted to induce lignin peroxidase (LiP) ... Keyword(s): cellulose , lignin peroxidase , LiP, lignin peroxidase , NBT, nitro blue tetrazolium , oxidant stress , ... 1991; Depolymerization of a synthetic lignin in vitro by lignin peroxidase. Enzyme Microb Technol13:15-18[CrossRef] ... 1994; Physiology and molecular biology of the lignin peroxidases of Phanerochaete chrysosporium. FEMS Microbiol Rev13:137-152[ ...
Cross-linking of tyrosine-containing peptides by hydrogen peroxide-activated Coprinus Cinereus peroxidase. In: European Food ... Cross-linking of tyrosine-containing peptides by hydrogen peroxide-activated Coprinus Cinereus peroxidase. / Steffensen, C.L. ( ... keywords = "Coprinus Cinereus peroxidase, Cross-linking, ESR, MALDI-tof MS, FTIR, Tyrosyl radical", ... In the forwarded hypothesis for the reaction mechanism upon peroxidase-initiated cross-linking of tyrosine-containing peptides ...
Crystal Structure Analysis of Fungal Versatile Peroxidase From Pleurotus Eryngii ... The binding sites of Arsenic atom in the structure of Crystal Structure Analysis of Fungal Versatile Peroxidase From Pleurotus ... Arsenic in the structure of Crystal Structure Analysis of Fungal Versatile Peroxidase From Pleurotus Eryngii (pdb 3fmu). ...
Pleurotus / laccase / manganese peroxidase / versatile peroxidase / submerged fermentation / solid-state fermentation. Source: ... Screening of laccase, manganese peroxidase, and versatile peroxidase activities of the genus Pleurotus in media with some raw ... Mn-dependent peroxidase (MnP), versatile peroxidase (VP), and the H2O2-generating enzyme aryl-alcohol oxidase, but not lignin ... peroxidases. Production of Lac and oxidation of 2,6-dimethoxyphenol (DMP) in the presence and absence of Mn2+ were detected ...
Peroxidase activity was no longer seen in the endoplasmic reticulum cisternae, whereas it was observed in portions of ... Peroxidase activity measured in the thyroid progressively decreased with prolonged administration of amitrole (Strum & ... In addition, fibrous thickening of both stroma and capsule was prominent and cisternal peroxidase activity was absent. Over 50- ... Most commonly this is the result of interference with the thyroid iodide transport system or interference with peroxidases ...
... the researchers identified the minimum amount of heating needed to inactivate both peroxidase and ascorbic-acid oxidase ...
Peroxidases,state=autocollapse}} *shows the template collapsed to the title bar if there is a {{navbar}}, a {{sidebar}}, or ... Peroxidases,state=collapsed}} to show the template collapsed, i.e., hidden apart from its title bar ... Peroxidases,state=expanded}} to show the template expanded, i.e., fully visible ... Retrieved from "https://en.wikipedia.org/w/index.php?title=Template:Peroxidases&oldid=704356592" ...
Glutathione peroxidase (GPx) (EC 1.11.1.9) is the general name of an enzyme family with peroxidase activity whose main ... glutathione peroxidase 4 (phospholipid hydroperoxidase) GPX5. Chr. 6 p21.32. glutathione peroxidase 5 (epididymal androgen- ... Glutathione peroxidase 2 is an intestinal and extracellular enzyme, while glutathione peroxidase 3 is extracellular, especially ... Glutathione peroxidase was discovered in 1957 by Gordon C. Mills.[8] Clinical significance[edit]. It has been shown that low ...
Inhibition of Peroxidase Activity of Cytochrome c: De Novo Compound Discovery and Validation. Ahmet Bakan, Alexandr A. Kapralov ... Inhibition of Cytochrome c Peroxidase Activity. Ahmet Bakan, Alexandr A. Kapralov, Hulya Bayir, Feizhou Hu, Valerian E. Kagan ... Inhibition of Cytochrome c Peroxidase Activity. Ahmet Bakan, Alexandr A. Kapralov, Hulya Bayir, Feizhou Hu, Valerian E. Kagan ... Pore opening is enhanced by peroxidase activity of cyt c gained upon its complexation with cardiolipin in the presence of ...
In eosinophil peroxidase deficiency, eosinophils have little or no eosinophil peroxidase. A lack of this protein does not seem ... Eosinophil peroxidase deficiency is estimated to occur in 8.6 in 1,000 Yemenite Jews, in 3 in 1,000 North-African Jews, and in ... Mutations in the EPX gene cause eosinophil peroxidase deficiency. The EPX gene provides instructions for making the eosinophil ... Eosinophil peroxidase deficiency is a condition that affects certain white blood cells called eosinophils but causes no health ...
The thyroid peroxidase antibodies test is primarily used to help diagnose and monitor autoimmune conditions involving the ... A thyroid peroxidase antibodies test checks the levels of antibodies made against the compound thyroid peroxidase (TPO) in the ... Thyroid peroxidase is an enzyme produced by the thyroid gland. The thyroid is a small, butterfly-shaped gland in the neck that ... The thyroid peroxidase antibodies test is considered a safe procedure. However, as with many medical tests, some problems can ...
Included is a discussion of peroxidases that also act as catalases and oxygenases. Heme Peroxidases serves as an essential text ... Discussed functions of peroxidases range from cell wall synthesis, synthesis of prostaglandins, role in drug suppression of ... Chapters written and edited by worldwide experts span a range of heme peroxidases from plants, yeast, bacteria and mammals. ... This book provides a comprehensive single source of information on the various aspects of heme peroxidase structure, function ...
Your basket is currently empty. i ,p>When browsing through different UniProt proteins, you can use the basket to save them, so that you can back to find or analyse them later.,p>,a href=/help/basket target=_top>More...,/a>,/p> ...
Thyroid peroxidase (TPO, antimicrosomal antibody, antithyroid microsomal antibody) antibody - blood. In: Chernecky CC, Berger ...
Definition of horseradish peroxidase. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and ... horseradish peroxidase. Definition: a peroxidases isolated from horseradish that is used in immunohistochemistry to label the ...
Supplementary Figure 3: Comparison of peroxidase staining conditions.. From: Multiplexed peroxidase-based electron microscopy ... Supplementary Figure 3: Comparison of peroxidase staining conditions. , Nature Neuroscience. ...
IPR010255 Haem_peroxidase_sf. IPR000823 Peroxidase_pln. IPR019794 Peroxidases_AS. IPR019793 Peroxidases_heam-ligand_BS. ... IPR010255 Haem_peroxidase_sf. IPR000823 Peroxidase_pln. IPR019794 Peroxidases_AS. IPR019793 Peroxidases_heam-ligand_BS. ... PS00435 PEROXIDASE_1, 1 hit. PS00436 PEROXIDASE_2, 1 hit. PS50873 PEROXIDASE_4, 1 hit. ... PS00435 PEROXIDASE_1, 1 hit. PS00436 PEROXIDASE_2, 1 hit. PS50873 PEROXIDASE_4, 1 hit. ...
... ,ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative ... TPO (Ab) EIA Thyroid Function 012-MDKAM-1 Thyroid Peroxidase. 7. TG (Ab) RIA Thyroid Function 014-HD 27.1 Thyroglobulin. 8. ... TPO (Ab) RIA Thyroid Function 014-HD-90.1 Thyroid Peroxidase. 4. TG (Ab) EIA Thyroid Function 012-MDKAT-1 Thyroglobulin anti-tg ...
Oxidoreductase, PeroxidaseUniRule annotation. ,p>Information which has been generated by the UniProtKB automatic annotation ... Thioredoxin-dependent peroxidase TPX1/2 (Fragment). Toxoplasma gondii GAB2-2007-GAL-DOM2 ... Belongs to the glutathione peroxidase family.UniRule annotation. Automatic assertion according to rulesi ... tr,Q6GVI1,Q6GVI1_TOXGO Glutathione peroxidase OS=Toxoplasma gondii OX=5811 PE=2 SV=1 ...
Animal heme-dependent peroxidases is a family of peroxidases. Peroxidases are found in bacteria, fungi, plants and animals. On ... eosinophil peroxidase (EPO); lactoperoxidase (LPO); thyroid peroxidase (TPO); prostaglandin H synthase (PGHS); and peroxidasin ... The peroxidase active site, which catalyzes the reduction of PGG2 to PGH2, is located on the other side of the molecule, at the ... Kimura S, Hong YS, Kotani T, Ohtaki S, Kikkawa F (1989). "Structure of the human thyroid peroxidase gene: comparison and ...
Animal heme-dependent peroxidases Ascorbate peroxidase Catalase Chloride peroxidase Cytochrome c peroxidase Glutathione ... A majority of peroxidase protein sequences can be found in the PeroxiBase database. Peroxidase can be used for treatment of ... Peroxidases are sometimes used as histological marker. Cytochrome c peroxidase is used as a soluble, easily purified model for ... The glutathione peroxidase family consists of 8 known human isoforms. Glutathione peroxidases use glutathione as an electron ...
... manganese peroxidase, versatile peroxidase and generic peroxidase), dye-decolorizing peroxidases, and heme-thiolate peroxidases ... They comprise three major groups: class II peroxidases (including lignin peroxidase, ... Manganese peroxidases and unspecific peroxygenases were found expressed in all nine investigated forest sites, and dye- ... Using this information, we were able to match, in environmental litter samples, two manganese peroxidase sequences to Mycena ...
glutathione peroxidase 1. Names. cellular glutathione peroxidase. selenium-dependent glutathione peroxidase 1. NP_032186.2. *EC ... GSH_Peroxidase; Glutathione (GSH) peroxidase family; tetrameric selenoenzymes that catalyze the reduction of a variety of ... Gpx1 glutathione peroxidase 1 [Mus musculus] Gpx1 glutathione peroxidase 1 [Mus musculus]. Gene ID:14775 ... glutathione peroxidase 1provided by MGI. Primary source. MGI:MGI:104887 See related. Ensembl:ENSMUSG00000063856 Vega: ...
Pentacoordination of the heme iron of Arthromyces ramosus peroxidase shown by a 1.8 A resolution crystallographic study at pH ... PEROXIDASE protein, length: 344 (BLAST) Sequence Similarity Cutoff. Rank. Chains in Cluster. Cluster ID / Name. Structural ...
  • In the forwarded hypothesis for the reaction mechanism upon peroxidase-initiated cross-linking of tyrosine-containing peptides and proteins, it is suggested that the polymerization takes place through a radical chain reaction. (vtt.fi)
  • The abilities of various peroxidases to catalyse the peroxidase-oxidase oxidation of seven aminothiols were studied. (biochemj.org)
  • Calculated ionisation potentials to determine the oxidation of vanillin precursors by lignin peroxidase. (wur.nl)
  • In view of the biocatalytic production of vanillin, this research focused on the lignin peroxidase (LiP) catalysed oxidation of naturally occurring phenolic derivatives: O-methyl ethers, O-acetyl esters, and O-glucosyl ethers. (wur.nl)
  • A 230-residues long homologous region exists in all eukaryotic members of Class I, which is present twice in both prokaryotic and archaeal catalase-peroxidases. (nih.gov)
  • The overall structure of eukaryotic Class I peroxidases may be retained in both halves of catalase-peroxidases, with major insertions in several loops, some of which may participate in inter-domain or inter-subunit interactions. (nih.gov)
  • The C-terminal halves of extant catalase-peroxidases clearly did not accumulate random changes, so prolonged periods of independent evolution of the duplicates can be ruled out. (nih.gov)
  • We suggest that the sparse representatives of eukaryotic catalase-peroxidases go back to lateral gene transfer, and that, except for several fungi, only single copy hydroperoxidases occur in the eukaryotic lineage. (nih.gov)
  • The bifunctional nature of catalase-peroxidases can be ascribed to several unique sequence peculiarities conserved among all N-terminal halves, which most likely will affect the properties of both haem ligands. (nih.gov)
  • In the current study, we functionally analysed these three catalase-peroxidase genes and found that MgCatD-1, encoding a secreted catalase-peroxidase, plays an important role in the pathogenicity of Z. tritici. (wur.nl)
  • These results show that secreted catalase-peroxidase is an important pathogenicity factor for successful pathogenesis of Z. tritici. (wur.nl)
  • These include superoxide dismutase ( 29 ), catalase ( 7 , 39 ), cytochrome c peroxidases ( 1 ), and alkyl hydroperoxide-reductase (AhpC) ( 2 ). (asm.org)
  • Molecular model of a tryparedoxin peroxidase molecule from the parasitic protozoa Trypanosoma cruzi, the cause of Chagas disease. (sciencephoto.com)
  • Thyroid Peroxidase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain containing 834 amino acids and having a molecular mass of 92,872 Dalton (excluding glycosylation), 101 kDa total mass. (prospecbio.com)
  • All five peroxidases were strongly glycosylated (3.6% to 4.9% N -glucosamine), with apparent molecular masses between 46 and 54 kD and pI values between pH 3.1 and 3.8. (plantphysiol.org)
  • This phenomenon complicates the determination of physiological roles for peroxidases when using a molecular biology approach. (plantphysiol.org)
  • This book provides a comprehensive single source of information on the various aspects of heme peroxidase structure, function and mechanism of action. (rsc.org)
  • It is suggested that adult female Anopheles albimanus mosquitoes contain a salivary heme peroxidase that functions during blood finding and blood feeding by destroying hemostatically active biogenic amines released by the vertebrate host during tissue destruction. (biologists.org)
  • Dr Robert Mason, Research Associate in the Department of Genetics, and first author of the study, said: 'In addition to glutathione peroxidase, this study has identified many genes that improve Huntington's 'symptoms' in yeast. (news-medical.net)
  • Photosynthetic electron transport regulates the expression of cytosolic ascorbate peroxidase genes in Arabidopsis during excess light stress. (semanticscholar.org)
  • Single knockout mutations in either one of two related thiol peroxidase genes, tpx and bcp , resulted in normal microaerobic growth (10% [vol/vol] oxygen) but poorer growth than that of the wild type under high-aeration conditions (21% [vol/vol] oxygen). (asm.org)
  • an oxidase layer atop the deposited colloidal gold adsorbed peroxidase wherein direct electron transfer to the conducting surface occurs in the presence of an analyte when the bioelectrode is suitably coupled with a reference/counter electrode. (google.com)
  • Peroxidases that utilize ASCORBIC ACID as an electron donor to reduce HYDROGEN PEROXIDE to WATER. (semanticscholar.org)
  • In the presence of peroxidases, TMB can act as an electron donor for the conversion of peroxides to water, changing the color of solution to a blue color equivalent to the degree of reactivity. (rockland-inc.com)
  • The variations in direct peroxidase bioelectrocatalysis when coming from carbon/graphite to metal electrodes and oxides, as well as modified electrodes, are analyzed regarding the variations in adsorption/orientation of peroxidase at the electrodes, interfacial electron transfer rates and mechanism of catalysis. (lu.se)
  • A typical group of peroxidases are the haloperoxidases. (wikipedia.org)
  • Extraction and partial characterization of peroxidase from Copaifera langsdorffii Desf. (scielo.br)
  • As peroxidases (E.C. 1.11.1.7, doador H 2 O 2 oxidorredutase) catalisam a redução do peróxido de hidrogênio ou outro peróxido orgânico, enquanto um doador de elétrons é oxidado. (scielo.br)
  • Peroxidases are widely believed to catalyze the last enzymatic step in the biosynthesis of lignin, the dehydrogenation of the p -coumaryl alcohols. (plantphysiol.org)
  • Since progesterone was found to inhibit peroxidase induction due to both estrone and diethylstilbestrol, as well as estradiol, it is considered unlikely that this antagonism relates to progestin-induced increases in uterine 17 β -hydroxysteroid dehydrogenase. (springer.com)
  • Isoflavones also inhibit thyroid peroxidase , which produces the thyroid hormones T3 and T4. (wordnik.com)