An enzyme isolated from horseradish which is able to act as an antigen. It is frequently used as a histochemical tracer for light and electron microscopy. Its antigenicity has permitted its use as a combined antigen and marker in experimental immunology.
An enzyme catalyzing the oxidation of 2 moles of glutathione in the presence of hydrogen peroxide to yield oxidized glutathione and water. EC 1.11.1.9.
A hemeprotein from leukocytes. Deficiency of this enzyme leads to a hereditary disorder coupled with disseminated moniliasis. It catalyzes the conversion of a donor and peroxide to an oxidized donor and water. EC 1.11.1.7.
A hemeprotein which catalyzes the oxidation of ferrocytochrome c to ferricytochrome c in the presence of hydrogen peroxide. EC 1.11.1.5.
Peroxidases that utilize ASCORBIC ACID as an electron donor to reduce HYDROGEN PEROXIDE to WATER. The reaction results in the production of monodehydroascorbic acid and DEHYDROASCORBIC ACID.
A 66-kDa peroxidase found in EOSINOPHIL granules. Eosinophil peroxidase is a cationic protein with a pI of 10.8 and is comprised of a heavy chain subunit and a light chain subunit. It possesses cytotoxic activity towards BACTERIA and other organisms, which is attributed to its peroxidase activity.
A hemeprotein that catalyzes the oxidation of the iodide radical to iodine with the subsequent iodination of many organic compounds, particularly proteins. EC 1.11.1.8.
A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials.
An agent thought to have disinfectant properties and used as an expectorant. (From Martindale, The Extra Pharmacopoeia, 30th ed, p747)
An oxidoreductase that catalyzes the conversion of HYDROGEN PEROXIDE to water and oxygen. It is present in many animal cells. A deficiency of this enzyme results in ACATALASIA.
An element with the atomic symbol Se, atomic number 34, and atomic weight 78.96. It is an essential micronutrient for mammals and other animals but is toxic in large amounts. Selenium protects intracellular structures against oxidative damage. It is an essential component of GLUTATHIONE PEROXIDASE.
An enzyme derived from cow's milk. It catalyzes the radioiodination of tyrosine and its derivatives and of peptides containing tyrosine.
A family of ubiquitously-expressed peroxidases that play a role in the reduction of a broad spectrum of PEROXIDES like HYDROGEN PEROXIDE; LIPID PEROXIDES and peroxinitrite. They are found in a wide range of organisms, such as BACTERIA; PLANTS; and MAMMALS. The enzyme requires the presence of a thiol-containing intermediate such as THIOREDOXIN as a reducing cofactor.
The most abundant natural aromatic organic polymer found in all vascular plants. Lignin together with cellulose and hemicellulose are the major cell wall components of the fibers of all wood and grass species. Lignin is composed of coniferyl, p-coumaryl, and sinapyl alcohols in varying ratios in different plant species. (From Merck Index, 11th ed)
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
An oxidoreductase that catalyzes the reaction between superoxide anions and hydrogen to yield molecular oxygen and hydrogen peroxide. The enzyme protects the cell against dangerous levels of superoxide. EC 1.15.1.1.
An enzyme that catalyzes the chlorination of a range of organic molecules, forming stable carbon-chloride bonds. EC 1.11.1.10.
A phylum of fungi that produce their sexual spores (basidiospores) on the outside of the basidium. It includes forms commonly known as mushrooms, boletes, puffballs, earthstars, stinkhorns, bird's-nest fungi, jelly fungi, bracket or shelf fungi, and rust and smut fungi.
Alcohols derived from the aryl radical (C6H5CH2-) and defined by C6H5CHOH. The concept includes derivatives with any substituents on the benzene ring.
Naturally occurring or synthetic substances that inhibit or retard the oxidation of a substance to which it is added. They counteract the harmful and damaging effects of oxidation in animal tissues.
Catalyzes the oxidation of GLUTATHIONE to GLUTATHIONE DISULFIDE in the presence of NADP+. Deficiency in the enzyme is associated with HEMOLYTIC ANEMIA. Formerly listed as EC 1.6.4.2.
A disturbance in the prooxidant-antioxidant balance in favor of the former, leading to potential damage. Indicators of oxidative stress include damaged DNA bases, protein oxidation products, and lipid peroxidation products (Sies, Oxidative Stress, 1991, pxv-xvi).
The color-furnishing portion of hemoglobin. It is found free in tissues and as the prosthetic group in many hemeproteins.
A group of compounds that contain a bivalent O-O group, i.e., the oxygen atoms are univalent. They can either be inorganic or organic in nature. Such compounds release atomic (nascent) oxygen readily. Thus they are strong oxidizing agents and fire hazards when in contact with combustible materials, especially under high-temperature conditions. The chief industrial uses of peroxides are as oxidizing agents, bleaching agents, and initiators of polymerization. (From Hawley's Condensed Chemical Dictionary, 11th ed)
A tripeptide with many roles in cells. It conjugates to drugs to make them more soluble for excretion, is a cofactor for some enzymes, is involved in protein disulfide bond rearrangement and reduces peroxides.
Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.
Peroxidase catalyzed oxidation of lipids using hydrogen peroxide as an electron acceptor.
The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
The rate dynamics in chemical or physical systems.
A genus of fungi in the family Corticiaceae, order Stereales, that degrades lignin. The white-rot fungus Phanerochaete chrysosporium is a frequently used species in research.
Very toxic industrial chemicals. They are absorbed through the skin, causing lethal blood, bladder, liver, and kidney damage and are potent, broad-spectrum carcinogens in most species.
A genus of black-spored basidiomycetous fungi of the family Coprinaceae, order Agaricales; some species are edible.
Inorganic binary compounds of iodine or the I- ion.
Selenoproteins are proteins that specifically incorporate SELENOCYSTEINE into their amino acid chain. Most selenoproteins are enzymes with the selenocysteine residues being responsible for their catalytic functions.
Peroxides produced in the presence of a free radical by the oxidation of unsaturated fatty acids in the cell in the presence of molecular oxygen. The formation of lipid peroxides results in the destruction of the original lipid leading to the loss of integrity of the membranes. They therefore cause a variety of toxic effects in vivo and their formation is considered a pathological process in biological systems. Their formation can be inhibited by antioxidants, such as vitamin E, structural separation or low oxygen tension.
The lectin wheatgerm agglutinin conjugated to the enzyme HORSERADISH PEROXIDASE. It is widely used for tracing neural pathways.
A genus of basidiomycetous fungi, family POLYPORACEAE, order POLYPORALES, that grows on logs or tree stumps in shelflike layers. The species P. ostreatus, the oyster mushroom, is a choice edible species and is the most frequently encountered member of the genus in eastern North America. (Alexopoulos et al., Introductory Mycology, 4th ed, p531)
A selenium compound with the molecular formula H2SO3. It used as a source of SELENIUM, especially for patients that develop selenium deficiency following prolonged PARENTERAL NUTRITION.
Highly reactive molecules with an unsatisfied electron valence pair. Free radicals are produced in both normal and pathological processes. They are proven or suspected agents of tissue damage in a wide variety of circumstances including radiation, damage from environment chemicals, and aging. Natural and pharmacological prevention of free radical damage is being actively investigated.
The dialdehyde of malonic acid.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A peroxiredoxin that is a cytosolic bifunctional enzyme. It functions as a peroxiredoxin via a single redox-active cysteine and also contains a Ca2+-independent acidic phospholipase A2 activity.
The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.
A highly vascularized endocrine gland consisting of two lobes joined by a thin band of tissue with one lobe on each side of the TRACHEA. It secretes THYROID HORMONES from the follicular cells and CALCITONIN from the parafollicular cells thereby regulating METABOLISM and CALCIUM level in blood, respectively.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.
A THIOREDOXIN-dependent hydroperoxidase that is localized in the mitochondrial matrix. The enzyme plays a crucial role in protecting mitochondrial components from elevated levels of HYDROGEN PEROXIDE.
An extracellular selenoprotein that contains most of the SELENIUM in PLASMA. Selenoprotein P functions as an antioxidant and appears to transport selenium from the LIVER to peripheral tissues.
Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.
Proteins that contain an iron-porphyrin, or heme, prosthetic group resembling that of hemoglobin. (From Lehninger, Principles of Biochemistry, 1982, p480)
A naturally occurring amino acid in both eukaryotic and prokaryotic organisms. It is found in tRNAs and in the catalytic site of some enzymes. The genes for glutathione peroxidase and formate dehydrogenase contain the TGA codon, which codes for this amino acid.
A family of bracket fungi, order POLYPORALES, living in decaying plant matter and timber.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
A six carbon compound related to glucose. It is found naturally in citrus fruits and many vegetables. Ascorbic acid is an essential nutrient in human diets, and necessary to maintain connective tissue and bone. Its biologically active form, vitamin C, functions as a reducing agent and coenzyme in several metabolic pathways. Vitamin C is considered an antioxidant.
An order of fungi in the phylum BASIDIOMYCOTA having macroscopic basidiocarps. The members are characterized by their saprophytic activities as decomposers, particularly in the degradation of CELLULOSE and LIGNIN. A large number of species in the order have been used medicinally. (From Alexopoulos, Introductory Mycology, 4th ed, pp504-68)
Molecules or ions formed by the incomplete one-electron reduction of oxygen. These reactive oxygen intermediates include SINGLET OXYGEN; SUPEROXIDES; PEROXIDES; HYDROXYL RADICAL; and HYPOCHLOROUS ACID. They contribute to the microbicidal activity of PHAGOCYTES, regulation of signal transduction and gene expression, and the oxidative damage to NUCLEIC ACIDS; PROTEINS; and LIPIDS.
Low-molecular-weight end products, probably malondialdehyde, that are formed during the decomposition of lipid peroxidation products. These compounds react with thiobarbituric acid to form a fluorescent red adduct.
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
An enzyme of the oxidoreductase class that catalyzes the conversion of beta-D-glucose and oxygen to D-glucono-1,5-lactone and peroxide. It is a flavoprotein, highly specific for beta-D-glucose. The enzyme is produced by Penicillium notatum and other fungi and has antibacterial activity in the presence of glucose and oxygen. It is used to estimate glucose concentration in blood or urine samples through the formation of colored dyes by the hydrogen peroxide produced in the reaction. (From Enzyme Nomenclature, 1992) EC 1.1.3.4.
Inorganic salts of HYDROGEN CYANIDE containing the -CN radical. The concept also includes isocyanides. It is distinguished from NITRILES, which denotes organic compounds containing the -CN radical.
Granular leukocytes with a nucleus that usually has two lobes connected by a slender thread of chromatin, and cytoplasm containing coarse, round granules that are uniform in size and stainable by eosin.
Enzymes which are immobilized on or in a variety of water-soluble or water-insoluble matrices with little or no loss of their catalytic activity. Since they can be reused continuously, immobilized enzymes have found wide application in the industrial, medical and research fields.
A trace element with atomic symbol Mn, atomic number 25, and atomic weight 54.94. It is concentrated in cell mitochondria, mostly in the pituitary gland, liver, pancreas, kidney, and bone, influences the synthesis of mucopolysaccharides, stimulates hepatic synthesis of cholesterol and fatty acids, and is a cofactor in many enzymes, including arginase and alkaline phosphatase in the liver. (From AMA Drug Evaluations Annual 1992, p2035)
A transferase that catalyzes the addition of aliphatic, aromatic, or heterocyclic FREE RADICALS as well as EPOXIDES and arene oxides to GLUTATHIONE. Addition takes place at the SULFUR. It also catalyzes the reduction of polyol nitrate by glutathione to polyol and nitrite.
An extensive order of basidiomycetous fungi whose fruiting bodies are commonly called mushrooms.
A direct-acting oxidative stress-inducing agent used to examine the effects of oxidant stress on Ca(2+)-dependent signal transduction in vascular endothelial cells. It is also used as a catalyst in polymerization reactions and to introduce peroxy groups into organic molecules.
A plant genus of the family BRASSICACEAE known for the root used in hot SPICES. It is also the source of HORSERADISH PEROXIDASE which is widely used in laboratories.
Organic derivatives of thiocyanic acid which contain the general formula R-SCN.
Diagnostic aid in pancreas function determination.
An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.
Inorganic salts of the hypothetical acid ferrocyanic acid (H4Fe(CN)6).
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Electron-accepting molecules in chemical reactions in which electrons are transferred from one molecule to another (OXIDATION-REDUCTION).
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Benzene derivatives that include one or more hydroxyl groups attached to the ring structure.
A copper-containing oxidoreductase enzyme that catalyzes the oxidation of 4-benzenediol to 4-benzosemiquinone. It also has activity towards a variety of O-quinols and P-quinols. It primarily found in FUNGI and is involved in LIGNIN degradation, pigment biosynthesis and detoxification of lignin-derived products.
Analysis of the intensity of Raman scattering of monochromatic light as a function of frequency of the scattered light.
The engulfing of liquids by cells by a process of invagination and closure of the cell membrane to form fluid-filled vacuoles.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
A GLUTATHIONE dimer formed by a disulfide bond between the cysteine sulfhydryl side chains during the course of being oxidized.
A group of compounds that are derivatives of methoxybenzene and contain the general formula R-C7H7O.
Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The disodium salt of selenious acid. It is used therapeutically to supply the trace element selenium and is prepared by the reaction of SELENIUM DIOXIDE with SODIUM HYDROXIDE.
A non-selective post-emergence, translocated herbicide. According to the Seventh Annual Report on Carcinogens (PB95-109781, 1994) this substance may reasonably be anticipated to be a carcinogen. (From Merck Index, 12th ed) It is an irreversible inhibitor of CATALASE, and thus impairs activity of peroxisomes.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
Inflammatory disease of the THYROID GLAND due to autoimmune responses leading to lymphocytic infiltration of the gland. It is characterized by the presence of circulating thyroid antigen-specific T-CELLS and thyroid AUTOANTIBODIES. The clinical signs can range from HYPOTHYROIDISM to THYROTOXICOSIS depending on the type of autoimmune thyroiditis.
Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.
Salts of hydrobromic acid, HBr, with the bromine atom in the 1- oxidation state. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A generic descriptor for all TOCOPHEROLS and TOCOTRIENOLS that exhibit ALPHA-TOCOPHEROL activity. By virtue of the phenolic hydrogen on the 2H-1-benzopyran-6-ol nucleus, these compounds exhibit varying degree of antioxidant activity, depending on the site and number of methyl groups and the type of ISOPRENOIDS.
Hydrogen-donating proteins that participates in a variety of biochemical reactions including ribonucleotide reduction and reduction of PEROXIREDOXINS. Thioredoxin is oxidized from a dithiol to a disulfide when acting as a reducing cofactor. The disulfide form is then reduced by NADPH in a reaction catalyzed by THIOREDOXIN REDUCTASE.
A genus of fungi in the family Coriolaceae.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
Organic compounds which contain selenium as an integral part of the molecule.
Proteins prepared by recombinant DNA technology.
A FLAVOPROTEIN enzyme that catalyzes the oxidation of THIOREDOXINS to thioredoxin disulfide in the presence of NADP+. It was formerly listed as EC 1.6.4.5
A group of physiologically active prostaglandin endoperoxides. They are precursors in the biosynthesis of prostaglandins and thromboxanes. Most frequently encountered member of this group is the prostaglandin G2.
The measurement of the amplitude of the components of a complex waveform throughout the frequency range of the waveform. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Compounds containing the -SH radical.
Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.
Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)
Diazo derivatives of aniline, used as a reagent for sugars, ketones, and aldehydes. (Dorland, 28th ed)
A plant genus in the family LILIACEAE (sometimes placed in Asparagaceae) that contains ECDYSTEROIDS and is an ingredient of Siotone. The shoots are used as a vegetable and the roots are used in FOLK MEDICINE.
Elements of limited time intervals, contributing to particular results or situations.
Hydroxycinnamic acid and its derivatives. Act as activators of the indoleacetic acid oxidizing system, thereby producing a decrease in the endogenous level of bound indoleacetic acid in plants.
The facilitation of biochemical reactions with the aid of naturally occurring catalysts such as ENZYMES.
An antiseptic and disinfectant aromatic alcohol.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.
A cytochrome oxidase inhibitor which is a nitridizing agent and an inhibitor of terminal oxidation. (From Merck Index, 12th ed)
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
A product from the iodination of MONOIODOTYROSINE. In the biosynthesis of thyroid hormones, diiodotyrosine residues are coupled with other monoiodotyrosine or diiodotyrosine residues to form T4 or T3 thyroid hormones (THYROXINE and TRIIODOTHYRONINE).
The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.

Stimulation of renin release from rabbit renal cortex by arachidonic acid and prostaglandin endoperoxides. (1/2940)

The mechanism by which renal prostaglandins stimulate renin secretion in vivo is unknown. In this in vitro study we measured the effects of activation of the prostaglandin (PG) system on renin release from slices of rabbit renal cortex. The PG precursor arachidonic acid (C20:4), a natural PG endoperoxide (PGG2), two stable synthetic PG endoperoxide analogues (EPA I and II), PGE2, PGF2alpha, and two different PG synthesis inhibitors [indomethacin and 5,8,11,14-eicosatetraynoic acid (ETA)] were used to evaluate the possibility of a direct action of the cortical PG system on renin secretion. Renin release increased significantly with time after addition of C20:4, PGG2, EPA I, and EPA II to the incubation medium. Stimulation of renin release was se-related for C20:4 in concentrations of 0.6 to 4.5 X 10(-6) M, for EPA I in concentrations of 0.7 to 2.8 X 10(-6) M, and for EPA II in concentrations of 1.4 to 14.0 X 10(-6) M. Indomethacin (10(-4) M) and ETA (10(-4) M) significantly decreased basal renin release as well as the renin release stimulated by C20:4 and EPA I. PGE2(10(-12) to 10(-6) M) had no effect on renin release, whereas PGF2alpha (10(-12) to 10(-6) M) decreased renin release in a dose-dependent manner. These data raise the possibility of a direct action of the renal cortical PG system on renin secretion. The results further indicate that stimulation of renin release by C20:4 may depend more specifically on the action of PG endoperoxides than on the primary prostaglandins.  (+info)

Isolation and purification of rat mammary tumor peroxidase. (2/2940)

7,12-Dimethylbenz(a)anthracene-induced rat mammary tumors often contain high levels of the enzyme perioxidase, a putative marker of estrogen dependence. This enzyme can be effectively extracted with 0.5 M CaCl2, giving rise to a soluble peroxidase with a molecular weight of about 50,000 as determined by gel filtration. This is the same size as the estrogen-induced peroxidase of rat uterus but smaller than other mammalian peroxidases. Further purification of the rat mammary tumor peroxidase by concanavalin A-Sepharose chromatography and hydrophobic interaction chromatography on phenyl Sepharose provides a 640-fold purification of the enzyme.  (+info)

Cloning of the peroxiredoxin gene family in rats and characterization of the fourth member. (3/2940)

Peroxiredoxin (PRx) exhibits thioredoxin-dependent peroxidase activity and constitutes a family of proteins. Four members of genes from rat tissues were isolated by PCR using degenerated primers based on the sequences which encode a pair of highly conserved Cys-containing domains, and were then cloned to full-length cDNAs. These included two genes which have previously been isolated in rats, PRx I and PRx II, and two rat homologues of PRx III and PRx IV. We showed, for the first time, the simultaneous expression of all four genes in various rat tissues by Northern blotting. Since a discrepancy exists regarding cellular distribution, we further characterized PRx IV by expressing it in COS-1 cells. This clearly demonstrates that PRx IV is a secretory form and functions within the extracellular space.  (+info)

Purification and characterization of a novel peroxidase from Geotrichum candidum dec 1 involved in decolorization of dyes. (4/2940)

A peroxidase (DyP) involved in the decolorization of dyes and produced by the fungus strain Geotrichum candidum Dec 1 was purified. DyP, a glycoprotein, is glycosylated with N-acetylglucosamine and mannose (17%) and has a molecular mass of 60 kDa and an isoelectric point (pI) of 3.8. The absorption spectrum of DyP exhibited a Soret band at 406 nm corresponding to a hemoprotein, and its Na2S2O4-reduced form revealed a peak at 556 nm that indicates the presence of a protoheme as its prosthetic group. Nine of the 21 types of dyes that were decolorized by Dec 1 cells were decolorized by DyP; in particular, anthraquinone dyes were highly decolorized. DyP also oxidized 2,6-dimethoxyphenol and guaiacol but not veratryl alcohol. The optimal temperature for DyP activity was 30 degrees C, and DyP activity was stable even after incubation at 50 degrees C for 11 h.  (+info)

Phospholipid hydroperoxide cysteine peroxidase activity of human serum albumin. (5/2940)

Human serum albumin (HSA) reduced the phospholipid hydroperoxide, 1-palmitoyl-2-(13-hydroperoxy-cis-9, trans-11-octadecadienoyl)-l-3-phosphatidylcholine (PLPC-OOH) to the corresponding hydroxy-derivative with a high apparent affinity (Km=9. 23+/-0.95 microM). Removal of bound lipid during purification increased this activity. At physiological concentration, HSA reduced the phospholipid hydroperoxide in the absence of a cofactor. However, in the presence of a cofactor (reductant), the rate of the reaction was increased. All of the major aminothiols in plasma could act as reductants, the best being the most abundant, cysteine (Km=600+/-80 microM). For every nanomole of PLPC-OOH reduced by HSA, 1.26 nmol of cystine was formed, indicating a reaction stoichiometry of 1 mol PLPC-OOH to 2 mol cysteine. We used chemical modification to determine which amino acid residues on HSA were responsible for the activity. Oxidation of thiol group(s) by N-ethylmaleimide led to a reduction in the rate of activity, whereas reduction of thiols by either dithiothreitol or the angiotensin-converting enzyme inhibitor, captopril, increased the activity. Both N-ethylmaleimide-modified HSA and dithiothreitol-treated HSA exhibited increased apparent affinities for PLPC-OOH. For a range of preparations of albumin with different modifications, the activity on PLPC-OOH was dependent on the amount of free thiol groups on the albumin (correlation coefficient=0.91). Patients with lowered albumin concentrations after septic shock showed lowered total plasma thiol concentrations and decreased phospholipid hydroperoxide cysteine peroxidase (PHCPx) activities. These results therefore show for the first time that HSA exhibits PHCPx activity, and that the majority of the activity depends on the presence of reduced thiol group(s) on the albumin.  (+info)

Use of site-directed mutagenesis to probe the structure, function and isoniazid activation of the catalase/peroxidase, KatG, from Mycobacterium tuberculosis. (6/2940)

A series of mutants bearing single amino acid substitutions often encountered in the catalase/peroxidase, KatG, from isoniazid-resistant isolates of Mycobacterium tuberculosis has been produced by site-directed mutagenesis. The resultant enzymes were overexpressed, purified and characterized. Replacing Cys-20 by Ser abolished disulphide-bridge formation, but did not affect either dimerization of the enzyme or catalysis. The substitution of Thr-275, which is probably involved in electron transfer from the haem, by proline resulted in a highly unstable enzyme with insignificant enzyme activities. The most commonly occurring substitution in drug-resistant clinical isolates is the replacement of Ser-315 by Thr; this lowered catalase and peroxidase activities by 50% and caused a significant decrease in the KatG-mediated inhibition of the activity of the NADH-dependent enoyl-[acyl-carrier protein] reductase, InhA, in vitro. The ability of this enzyme to produce free radicals from isoniazid was severely impaired, as judged by its loss of NitroBlue Tetrazolium reduction activity. Replacement of Leu-587 by Pro resulted in marked instability of KatG, indicating that the C-terminal domain is also important for structural and functional integrity.  (+info)

Direct interaction of lignin and lignin peroxidase from Phanerochaete chrysosporium. (7/2940)

Binding properties of lignin peroxidase (LiP) from the basidiomycete Phanerochaete chrysosporium against a synthetic lignin (dehydrogenated polymerizate, DHP) were studied with a resonant mirror biosensor. Among several ligninolytic enzymes, only LiP specifically binds to DHP. Kinetic analysis revealed that the binding was reversible, and that the dissociation equilibrium constant was 330 microM. The LiP-DHP interaction was controlled by the ionization group with a pKa of 5.3, strongly suggesting that a specific amino acid residue plays a role in lignin binding. A one-electron transfer from DHP to oxidized intermediates LiP compounds I and II (LiPI and LiPII) was characterized by using a stopped-flow technique, showing that binding interactions of DHP with LiPI and LiPII led to saturation kinetics. The dissociation equilibrium constants for LiPI-DHP and LiPII-DHP interactions were calculated to be 350 and 250 microM, and the first-order rate constants for electron transfer from DHP to LiPI and to LiPII were calculated to be 46 and 16 s-1, respectively. These kinetic and spectral studies strongly suggest that LiP is capable of oxidizing lignin directly at the protein surface by a long-range electron transfer process. A close look at the crystal structure suggested that LiP possesses His-239 as a possible lignin-binding site on the surface, which is linked to Asp-238. This Asp residue is hydrogen-bonded to the proximal His-176. This His-Asp...proximal-His motif would be a possible electron transfer route to oxidize polymeric lignin.  (+info)

Eosinophil peroxidase increases membrane permeability in mammalian urinary bladder epithelium. (8/2940)

Eosinophil peroxidase (EPO), a cationic protein found in eosinophils, has been reported to be cytotoxic independent of its peroxidase activity. This study investigated with electrophysiological methods whether EPO is toxic to mammalian urinary bladder epithelium. Results indicate that EPO, when added to the mucosal solution, increases apical membrane conductance of urinary bladder epithelium only when the apical membrane potential is cell interior negative. The EPO-induced conductance was concentration dependent, with a maximum conductance of 411 microseconds/cm2 and a Michaelis-Menten constant of 113 nM. The EPO-induced conductance was nonselective for K+ and Cl-. The conductance was partially reversed using voltage but not by removal of EPO from the bulk solution. Mucosal Ca2+ reversed the EPO-induced conductance by a mechanism involving reversible block of the conductance. Prolonged exposure (up to 1 h) to EPO was toxic to the urinary bladder epithelium, as indicated by an irreversible increase in transepithelial conductance. These results suggest that EPO is indeed toxic to urinary bladder epithelium via a mechanism that involves an increase in membrane permeability.  (+info)

In order to understand the mechanism of molecular interactions at the active site of Tryparedoxin Peroxidase (Try P), homology modeling and docking studies were performed. We generated a Three-Dimensional (3D) model of target protein based on the Crystal structure of Leishmania Major Try PI (PDB ID: 3TUE) using modeler software. Docking analysis was carried out to study the effects of methotrexate on Tryparedoxin Peroxidase (Try P). Inhibition of the Tryparedoxin peroxidase interaction has become a new therapeutic strategy in treating leishmaniasis. Docking analysis was carried out to study the effects of methotrexate on Tryparedoxin Peroxidase (TryP). Tryparedoxin peroxidase of Trypanosomatidae family functions as antioxidant through their peroxidase and peroxynitrite reductase activities. The theoretical docking study, conducted on a sample previously reported for anti-cancer properties of Methotrexate at the binding site of 3D models of Tryparedoxin Peroxidase of Leishmania braziliensis (L. ...
TY - JOUR. T1 - Expression of a moderately anionic peroxidase is induced by aluminum treatment in tobacco cells. T2 - Possible involvement of peroxidase isozymes in aluminum ion stress. AU - Ezaki, Bunichi. AU - Tsugita, Shinobu. AU - Matsumoto, Hideaki. PY - 1996/1. Y1 - 1996/1. N2 - To clarify the mechanism of aluminum (Al) toxicity and Al tolerance, we isolated a new clone (pAL201) from a tobacco cDNA library. Northern blot hybridization analysis indicated that the expression of pAL201 is induced by Al treatment and phosphate (Pi) starvation. The complete cDNA sequence suggested that this clone encodes a moderately anionic peroxidase (EC 1.11.1.7). Analysis by isoelectric focussing indicated that a moderately anionic peroxidase (approximately pI 6.7) and two cationic peroxidases (pI 9.2 and 9.7) in the soluble fraction are activated by Al treatment and Pi starvation, while two moderately anionic isozymes are repressed by these stresses. We suppose that Al ion stress can control the activity ...
Fungal secretory peroxidases mediate fundamental ecological functions in the conversion and degradation of plant biomass. Many of these enzymes have strong oxidizing activities towards aromatic compounds and are involved in the degradation of plant cell wall (lignin) and humus. They comprise three major groups: class II peroxidases (including lignin peroxidase, manganese peroxidase, versatile peroxidase and generic peroxidase), dye-decolorizing peroxidases, and heme-thiolate peroxidases (e.g. unspecific/aromatic peroxygenase, chloroperoxidase). Here, we have repeatedly observed a widespread expression of all major peroxidase groups in leaf and needle litter across a range of forest ecosystems (e.g. Fagus, Picea, Acer, Quercus, and Populus spp.), which are widespread in Europe and North America. Manganese peroxidases and unspecific peroxygenases were found expressed in all nine investigated forest sites, and dye-decolorizing peroxidases were observed in five of the nine sites, thereby indicating
Hydrogen peroxide is thought to regulate cellular processes by direct oxidation of numerous cellular proteins, whereas antioxidants, most notably thiol peroxidases, are thought to reduce peroxides and inhibit H2O2 response. However, thiol peroxidases have also been implicated in activation of transcription factors and signaling. It remains unclear if these enzymes stimulate or inhibit redox regulation and whether this regulation is widespread or limited to a few cellular components. Herein, we found that Saccharomyces cerevisiae cells lacking all eight thiol peroxidases were viable and withstood redox stresses. They transcriptionally responded to various redox treatments, but were unable to activate and repress gene expression in response to H2O2. Further studies involving redox transcription factors suggested that thiol peroxidases are major regulators of global gene expression in response to H2O2. The data suggest that thiol peroxidases sense and transfer oxidative signals to the signaling ...
Heme peroxidases are widely distributed in biological systems and are involved in a wide range of processes essential for life. This book provides a comprehensive single source of information on the various aspects of heme peroxidase structure, function and mechanism of action. Chapters written and edited by worldwide experts span a range of heme peroxidases from plants, yeast, bacteria and mammals. Discussed functions of peroxidases range from cell wall synthesis, synthesis of prostaglandins, role in drug suppression of tuberculosis, and antibacterial activity. Included is a discussion of peroxidases that also act as catalases and oxygenases. Heme Peroxidases serves as an essential text for those working in industry and academia in biochemistry and metallobiology.
Peroxidase Substrates for Western Blotting, Immunohistochemistry, and ELISAPeroxidase Enzymes and Reagents Technical Information
Minute Peroxidase Suppressor is designed to inhibit endogenous peroxidase activity commonly encountered in immunohistochemistry (IHC) procedures. Inhibiting endogenous peroxidase activity is essential for avoiding false positive and reduce the background of IHC. Many chemical agents such as H2O2 and sodium aside have been used for this purpose but the inhibition is usually incomplete. Minute TM HRP Suppressor is a mixture of several potent HRP inhibitors and the result is a complete inhibitionof endogenous peroxidase activity. The suppressor can be used in regular IHC as well as HRP-mediated super sensitive procedures such as tyramide signal amplification (TSA). Major Features: Very stable at RT, ready to use and irreversible inhibition of endogenous peroxidase activity. Significantly reduce the background of sensitive assays using HRP. 1. After cell or tissue samples are prepared on slide, perform antigen retrieval step if necessary. 2. Block non-specific sites in the samples with normal serum ...
Infinite Enzymes Manganese Peroxidase Enzyme (MnP) is a recombinant peroxidase from Phanerochaete chrysosporium produced in corn seed. Request Quote now!
Alterations in Soluble Class III Peroxidases of Maize Shoots by Flooding Stress. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Thiol peroxidases are critical enzymes in the redox control of cellular processes that function by reducing low levels of hydroperoxides and regulating redox signaling. These proteins were also shown to regulate genome stability, but how their dysfunction affects the actual mutations in the genome is not known. Saccharomyces cerevisiae has eight thiol peroxidases of glutathione peroxidase and peroxiredoxin families, and the mutant lacking all these genes (Δ8) is viable. In this study, we employed two independent Δ8 isolates to analyze the genome-wide mutation spectrum that results from deficiency in these enzymes. Deletion of these genes was accompanied by a dramatic increase in point mutations, many of which clustered in close proximity and scattered throughout the genome, suggesting strong mutational bias. We further subjected multiple lines of wild-type and Δ8 cells to long-term mutation accumulation, followed by genome sequencing and phenotypic characterization. Δ8 lines showed a ...
White-rot fungi (WRF) are capable of degrading complex organic compounds such as lignin, and the enzymes that enable these processes can be used for the detoxification of recalcitrant organopollutants. The aim of this study is to evaluate a system based on the use of an in vitro ligninolytic enzyme for the detoxification of recalcitrant dye pollutants. The dyes selected for investigation were the anionic and cationic commercial azo dyes, basic blue 41 (BB41), acid black 1 (AB1), and reactive black 5 (RB5). A supernatant, cell-free culture of WRF with manganese peroxidase activity was used to investigate its degradative capacity under various conditions, and concentrations of cofactors, H2O2 and Mn2+. The assays were carried out using a 22 experimental designs whose variables were concentration of Mn2+ (33 and 1,000 μM) and semicontinuous dosage of the H2O2 (0.02 and 0.10 μmol) added at a frequency of 0.2 min−1. The response variables analyzed were the efficiency and the initial rate of the ...
The main objective of this study was to investigate the effect of adding a pretreatment stage in conventional hydrogen peroxide bleaching of high yield pulps. To achieve this, three main variables considered crucial in the effectiveness of manganese peroxidase on the oxidation of lignin substructures, were chosen to evaluate the effect of this enzyme on high yield pulps. The responses to evaluate this effect were brightness, brightness reversion, pulp viscosity, and yield. Other properties included opacity, scattering and absorption coefficients, and the Hunter Lab values. The pulps were bleached using two sequences Q(MnP), and Q(MnP)QP, to assess the effect of the enzyme alone and when it was a pretreatment. Malonate buffer concentration, MnP charge, and pH were the three factors used and they were varied between two levels, high and low. An analysis of the data indicate that both sequences raised the brightnesses of the two pulps. Following Q(MnP)QP bleaching, brightness reversion was reduced, pulp
산화적 스트레스(Oxidative Stress)는 Reactive Oxygen Species (ROS)와 antioxidant (항산화제)간의 불균형에 의해 발생되며, ROS의 과도한 축적은 DNA, 단백질 및 지질 세포막의 손상과 같은 세포적 손상을 초래합니다.. 산화적 스트레스의 조건에서 발생되는 Hydrogen Peroxide (H2O2)와 같은 과산화물(peroxide)은 ROS의 대표적인 산물 중 하나로 진핵세포에서는 유독하며, 높은 농도에서는 DNA, 지질 단백질의 산화를 일으켜 돌연변이 유발이나 세포사멸을 일으키기도 합니다.. peroxide에 의한 세포적 손상은 노화, 천식, 관절염, 당뇨병, 심혈관 질환, 신경성 퇴행성 질환 등 여러 질환의 발병과도 관계가 있습니다. EZ-Hydrogen Peroxide/Peroxidase assay kit는 Oxi-Probe를 사용하여 Hydrogen Peroxide (H2O2) 또는 Peroxidase 활성을 측정할 수 있는 제품으로 간단한 실험 방법과 민감도를 가지는 제품입니다. 본 Kit는 ...
Title: Recents Patents in the Use of Peroxidases. VOLUME: 3 ISSUE: 2. Author(s):Berenize Alvarado and Eduardo Torres. Affiliation:Benemerita Universidad Autonoma de Puebla, Centro de Quimica-ICUAP. Ciudad Universitaria, Puebla, 72570. Mexico.. Keywords:Biosensor, enzymatic oxidation, peroxidases, review. Abstract: Peroxidases are hemoenzymes with a wide range of applications, from fine chemical synthesis to environmental biocatalysis. These outstanding biocatalysts are able to catalyze reactions such as heteroatom oxidation (Nand S-oxidation), epoxidation, hydroxylation, and the oxidation of alcohols and indole, often giving high yields and enantiomeric excess values. This makes these biocatalysts very useful for application to several biotechnological processes. In this paper, recent advances and patents surrounding the use of peroxidases are reviewed, covering different aspects related to the applications of peroxidases and the modifications carried out to improve their functionality as ...
casSAR Dugability of Q73WB6 | MAP_2744c | Catalase-related peroxidase - Also known as CRPE_MYCPA, MAP_2744c. Has an organic peroxide-dependent peroxidase activity. Exhibits strong peroxidase activity using organic hydroperoxides as cosubstrates, weak peroxidase activity using hydrogen peroxide and negligible catalase activity. May have a role in elimination of reactive oxygen species, in particular by deactivating hydroperoxides. Monomer.
There is another endogenous peroxidase blocking method the blocks peroxidases and pseudoperoxidases. It can be used for minimally fixed frozen sections and in your case, paraffin sections. The is very thorough and gentle. It is called Glucose Oxidase blocking method. We love it for frozens as it does not chew sections off the slide. We have not tried it on paraffin but I have it by private communication it will work with paraffin sections/NBF fixed. I have seen it reported as 30 min in the working solution but original publication used it for 60 min. I will be happy to attach the method to you, not via Histonet. At 09:21 PM 6/23/2004 -0400, you wrote: >Todd, > Youre right. Sorry I havent responded to everyones suggestions >on my posting on blocking endogenous peroxidase in paraffin >sections. I think those who suggested that biotin might be the >problem have a point, however when I ran control sections with >just buffer, blocking (10% goat serum), and DAB, I still got >brown, even though ...
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Hamster mast cells have been found to give strong peroxidatic reactions at pH 5, 7.5 and 10 when sections of skeletal muscle are incubated for 2.5 h in the dark at room temperature on semipermeable...
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Enzyme Explorer - Peroxidase Enzymes products. Sigma-Aldrichs peroxidase product is recognized around the world as the industry standard for diagnostic manufacturing and laboratory scale research applications.
DAB (3, 3 -diaminobenzidine) HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications. DAB chromogen is effective as a single label or as a second color for multiple antigen labeling.
DAB (3, 3 -diaminobenzidine) HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications.
1. Thaw plates before opening bags to prevent condensation in the wells. 2. Add 200ul Blocking Buffer to each well and incubate 1 hour @ room temperature (RT). 3. Wash 3x w/ PBST. Aspirate and blot dry. 4. Prepare standards and samples in PBS. 5. Add 100ul of dilutions to wells. 6. Incubate plate 2-3h @ RT. 7. Incubate o/n @ 4C. 8. Wash 3x w/ PBST. Aspirate and blot dry. 9. Dilute biotinylated mAB211 in PBST + 0.5% Bovine Serum Albumin (BSA). 10. Add 100ul diluted Ab to each well. Incubate 2h @ RT. 11. Wash 3x w/ PBST. Aspirate and blot dry. 12. Prepare HRP-Streptavidin...... 13. Add 100ul diluted HRP-Streptavidin to each well. Incubate 2h @ RT. 14. Wash 3x w/ PBST. Aspirate and blot dry. 15. Prepare Peroxidase Substrate immediately before use. 16. Add 100ul Peroxidase Substrate to each well and incubate 10min in the dark @ RT. 17. Add 100ul Stopper Solution to wells and read plate at 490nm. ...
peroxidase, putative; FUNCTIONS IN: electron carrier activity, peroxidase activity, heme binding; INVOLVED IN: response to oxidative stress; LOCATED IN: endomembrane system; EXPRESSED IN: 10 plant structures; EXPRESSED DURING: 6 growth stages; CONTAINS InterPro DOMAIN/s: Haem peroxidase (InterPro:IPR010255), Plant peroxidase (InterPro:IPR000823), Haem peroxidase, plant/fungal/bacterial (InterPro:IPR002016); BEST Arabidopsis thaliana protein match is: peroxidase, putative (TAIR:AT5G58400.1); Has 2891 Blast hits to 2875 proteins in 212 species: Archae - 0; Bacteria - 4; Metazoa - 2; Fungi - 66; Plants - 2777; Viruses - 0; Other Eukaryotes - 42 (source: NCBI BLink ...
Streptococcus pneumoniae is a facultative anaerobic pathogen. Although it maintains fermentative metabolism, during aerobic growth pneumococci produce high levels of H2O2, which can have adverse effects on cell viability and DNA, and influence pneumococcal interaction with its host. The pneumococcus is unusual in its dealing with toxic reactive oxygen species in that it neither has catalase nor the global regulators of peroxide stress resistance. Previously, we identified pneumococcal thiol peroxidase (TpxD) as the key enzyme for enzymatic removal of H2O2, and showed that TpxD synthesis is up-regulated upon exposure to H2O2. This study aimed to reveal the mechanism controlling TpxD expression under H2O2 stress. We hypothesize that H2O2 activates a transcription factor which in turn up-regulates tpxD expression. Microarray analysis revealed a pneumococcal global transcriptional response to H2O2. Mutation of tpxD abolished H2O2-mediated response to high H2O2 levels, signifying the need for an active TpxD
Peroxidase Conjugated Affinity Purified Anti-SHEEP IgG F(ab)2 (RABBIT), Peroxidase Conjugated Affinity Purified anti-Sheep IgG F(ab )2 [Rabbit]; N/A Peroxidase Conjugated Affinity Purified Anti-SHEEP IgG F(ab)2 (RABBIT)IGHG1
cis-regulatory elements of the peroxidase gene in Arabidopsis thaliana involved in root-specific expression and responsiveness to high-salt stress.: The pattern
The Determination of Peroxidase in Amniotic Fluid.: Analyzing total peroxidase activity in amniotic fluid is extremely simple, requiring only 1 1/2 minutes of i
Within biological systems iron is a transition metal that allows access to the benefits of molecular oxygen as an oxidant. However, with these benefits come grave consequences if the reactions are not strictly controlled. The most prominent strategy of control and specialization is the protein environment that surrounds iron. Within iron containing proteins, specifically heme proteins, there are four basic levels of structure that impact the irons function: cofactor structure, protein-supplied ligands, non-ligand active site environment, and protein features that are distant from the active site. This last level remains poorly understood due to a lack of good models to pursue such studies. Catalase-peroxidases are unique heme proteins because they catalyze peroxide decomposition by two separate mechanisms, catalase and peroxidase, using the same active site. However, were it not for three structural features distant from the active site, catalase-peroxidases would be practically superimposable ...
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SWISS-MODEL Template Library (SMTL) entry for 1u2l. Crystal structure of the C-terminal domain from the catalase-peroxidase KatG of Escherichia coli (P1)
1NHQ: Crystallographic analyses of NADH peroxidase Cys42Ala and Cys42Ser mutants: active site structures, mechanistic implications, and an unusual environment of Arg 303.
Purified Native R. communis Lectin Toxin RCA60, Peroxidase labeled from Creative Biomart. Native R. communis Lectin Toxin RCA60, Peroxidase labeled can be used for research.
1NHP: Crystallographic analyses of NADH peroxidase Cys42Ala and Cys42Ser mutants: active site structures, mechanistic implications, and an unusual environment of Arg 303.
в сборнике Proceeding. Plant Peroxidase. Biochem. and Physiology. IY Int. Symp, место издания Ed. C.Obinger, U.Burner, R.Ebermann, C.Penel, H.Greppin, University of Agriculture, Vienna, and University of Geneva, с. 198-203 ...
is this on human tissues? abizar www.innogenex.com 1-877-igx-info -----Original Message----- From: [email protected] [mailto:[email protected]] Sent: Wednesday, May 03, 2000 3:22 PM To: [email protected] Subject: controls for rabbit polyclonals Plea for help, I am unable to get clean control slides for any rabbit polyclonal antibodies on either paraffin or frozen sections. There is always background (often very dark) on the collagen, smooth muscle (especially prostate) etc. This happens with commercial antibodies (B2microglobulin or vonWillebrand), in house Abs, as well as with rabbit IgG. I use casein or superblock. Avidin-Biotin block and exhaust endogenous peroxidase. I use the ABC kit with DAB. There is no problem if I omit the primary antibody. Can anybody offer any insights? Thank you, Bonnie ...
Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Sodium atom in PDB 2b2r: Crystal Structure Of An Oxoferryl Species of Catalase- Peroxidase Katg At PH5.6
peroxidase: Any of a group of enzymes that occur especially in plant cells and catalyze the oxidation of a substance by a peroxide.
anti-Glutathione Peroxidase 7, Polyclonal, Novus Biologicals 0.1mL; Unlabeled Life Sciences:Antibodies:Primary Antibodies:Immunoprecipitation (IP)
Histofine Simple Stain MAX Peroxidase (Human Tissue, Mouse primary): 414131F by As One International, Inc. at Labscoop.com - Read reviews, citations, datasheets, protocols & more.
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Click on a taxon to see the following taxons, if there are any.. By placing the mouse over a node a tooltip will appear. This tootip contains the taxonomic path from Cellular organisms to the node and, in the case of a species, the number of peroxidases in the orthogroup, the total number of peroxidases for that species in the orthogroups class and where possible the names of those peroxidases. The common ancestor for the orthogroup is written in bold in the tooltip. Return to orthogroup ...
The orthogroup DiHCcP001 is composed of 84 sequences in 68 species. Click here to see a graphical representation of the species in this orthogroup.. ...
A cDNA for thylakoid-bound ascorbate peroxidase of pumpkin was cloned and characterized. Thylakoid-bound ascorbate peroxidase had a high similarity to cytosolic ascorbate peroxidases, and the precursor contained a transit peptide to chloroplasts at its ammo-terminus and a putative membrane-spanning region at its carboxy-terminus.. ...
The ionic strength dependence of the reduction of cytochrome c peroxidase compound I by horse cytochrome c has been studied, using stopped-flow technigue, in pH 7.5, potassium phosphate/nitrate buffer. The temperature was set at 25 ± 1° C. The wavelength was monitored at 4 32 nm, an isobestic point for ferri-/ferrocytochrome c, so only the absorbance change of the reduction of cytochrome c peroxidase compound I to compound II can be observed. The observed rate constant, kobs, as a function of the concentration of ferrocytochrome c shows a non-linear increase with increasing ionic strength. A two-parameter eguation is needed to fit the data at low ionic strength, 20 mM to 40 mM, while a three-parameter equation is needed at high ionic strength, 65 mM and above. The maximum rates of these reductions also show two different types of ionic strength dependence. At 20 mM to 40 mM ionic strength, the maximum rate of reduction decreases slightly, within experimental error, with increasing ionic ...
We previously demonstrated that manganese peroxidase from the white-rot fungus Phanerochaete chrysosporium was very susceptible to thermal inactivation due to the loss of calcium from the enzyme [Sutherland & Aust (1996) Arch. Biochem. Biophys. 332, 128-134]. The structural changes that occur during thermal inactivation and the release of calcium from manganese peroxidase have now been characterized. Thermal inactivation caused distinct alterations in the heme environment and slight changes in the overall protein structure, both of which were reversed upon reactivation of the enzyme with calcium. The absorption spectrum of inactivated manganese peroxidase was similar to that of low-spin ferric heme proteins, indicating that a sixth ligand had bound to the distal side of the heme iron. Consistent with disruption of the distal heme environment, thermally inactivated manganese peroxidase did not react with hydrogen peroxide to form compound I. The inactive enzyme exhibited a pH-dependent absorption
TY - JOUR. T1 - Crystal structure of lignin peroxidase. AU - Edwards, Steven L.. AU - Raag, Reetta. AU - Wariishi, Hiroyuki. AU - Gold, Michael H.. AU - Poulos, Thomas L.. PY - 1993/1/15. Y1 - 1993/1/15. N2 - The crystal structure of lignin peroxidase (LiP) from the basidiomycete Phanerochaete chrysosporium has been determined to 2.6 Å resolution by using multiple isomorphous replacement methods and simulated annealing refinement. Of the 343 residues, residues 3-335 have been accounted for in the electron density map, including four disulfide bonds. The overall three-dimensional structure is very similar to the only other peroxidase in this group for which a high-resolution crystal structure is available, cytochrome c peroxidase, despite the fact that the sequence identity is only ≈20%, LiP has four disulfide bonds, while cytochrome c peroxidase has none, and LiP is larger (343 vs. 294 residues). The basic helical fold and connectivity defined by 11 helical segments with the heme sandwiched ...
Species of the genus Pleurotus are among the most efficient natural species in lignin degradation belonging to the subclass of ligninolytic organisms that produce laccase (Lac), Mn-dependent peroxidase (MnP), versatile peroxidase (VP), and the H2O2-generating enzyme aryl-alcohol oxidase, but not lignin peroxidases. Production of Lac and oxidation of 2,6-dimethoxyphenol (DMP) in the presence and absence of Mn2+ were detected both in submerged fermentation (SF) of dry ground mandarine peels and in solid-state fermentation (SSF) of grapevine sawdust in all investigated Pleurotus species and strains. Evidence of cultivation methods having a distinct influence on the level of enzyme activities has been demonstrated. Most of the species and strains had higher Lac activity under SSF conditions than under SF conditions. DMP oxidation in the presence and absence of Mn2+ was detected in all investigated species and strains, but was lower under SF conditions than under SSF conditions for most of ...them. ...
Additional file 19: of Genome-wide and evolutionary analysis of the class III peroxidase gene family in wheat and Aegilops tauschii reveals that some members are involved in stress responses
Author(s): Smith, Martyn T.; Yager, J; Steinmetz, K; Eastmond, D | Abstract: The metabolism of two of benzenes phenolic metabolites, phenol and hydroquinone, by peroxidase enzymes has been studied in detail. Studies employing horseradish peroxidase and human myeloperoxidase have shown that in the presence of hydrogen peroxide phenol is converted to 4,4-diphenoquinone and other covalent binding metabolites, whereas hydroquinone is converted solely to 1,4-benzoquinone. Surprisingly, phenol stimulates the latter conversion rather than inhibiting it, an effect that may play a role in the in vivo myelotoxicity of benzene. Indeed, repeated coadministration of phenol and hydroquinone to B6C3F1 mice results in a dramatic and significant decrease in bone marrow cellularity similar to that observed following benzene exposure. A mechanism of benzene-induced myelotoxicity is therefore proposed in which the accumulation and interaction of phenol and hydroquinone in the bone marrow and the peroxidase-dependent
Mitochondrial swelling and membrane protein thiol oxidation associated with mitochondrial permeability transition induced by Ca2+ and inorganic phosphate are inhibited in a dose-dependent manner either by catalase, the thiol-specific antioxidant enzyme (TSA), a protein recently demonstrated to present thiol peroxidase activity, or ebselen, a selenium-containing heterocycle which also possesses thiol peroxidase activity. This inhibition of mitochondrial permeability transition is due to the removal of mitochondrial-generated H2O2, which can easily diffuse to the extramitochondrial space. Whereas ebselen required the presence of reduced glutathione as a reductant to grant its protective effect, TSA was fully reduced by mitochondrial components. Decrease in the oxygen concentration of the reaction medium also inhibits mitochondrial permeabilization and membrane protein thioloxidation, in a concentration-dependent manner. The results presented in this report confirm that mitochondrial permeability ...
Poole, L.B., and Ellis, H.R. (1996) Flavin-dependent alkyl hydroperoxide reductase from Salmonella typhimurium. 1. Purification and enzymatic activities of overexpressed AhpF and AhpC proteins. Biochemistry 35, 56-64. PDF of article Poole, L.B. (1996) Flavin-dependent alkyl hydroperoxide reductase from Salmonella typhimurium. 2. Cystine disulfides involved in catalysis of peroxide reduction. Biochemistry 35, 65-75. PDF of article Poole, L.B., Chae, H.Z., Flores, B.M., Reed, S.L., Rhee, S.G., and Torian, B.E. (1997) Peroxidase activity of a TSA-like antioxidant protein from a pathogenic amoeba. Free Radical Biol. Med. 23, 955-959. PDF of article Li Calzi, M., and Poole, L.B. (1997) Requirement for the two AhpF cystine disulfide centers in catalysis of peroxide reduction by alkyl hydroperoxide reductase. Biochemistry 36, 13357-13364. PDF of article Ellis, H. R., and Poole, L.B. (1997) Roles for the two cysteine residues of AhpC in catalysis of peroxide reduction by alkyl hydroperoxide reductase ...
thyroid peroxidase - MedHelps thyroid peroxidase Center for Information, Symptoms, Resources, Treatments and Tools for thyroid peroxidase. Find thyroid peroxidase information, treatments for thyroid peroxidase and thyroid peroxidase symptoms.
Kangasjärvi, Saijaliisa; Lepistö, Anna; Hannikainen, Kati; Piippo, Mirva; Luomala, Eeva-Maria; Aro, Eva-Mari; Rintamäki, Eevi (2008 ...
Lactoperoxidase is a peroxidase enzyme secreted from mammary, salivary, and other mucosal glands that functions as a natural antibacterial agent. Lactoperoxidase is a member of the heme peroxidase family of enzymes. In humans, lactoperoxidase is encoded by the LPO gene. Lactoperoxidase catalyzes the oxidation of a number of inorganic and organic substrates by hydrogen peroxide. These substrates include bromide and iodide and therefore lactoperoxidase can be categorised as a haloperoxidase. Another important substrate is thiocyanate. The oxidized products produced through the action of this enzyme have potent bactericidal activities. Lactoperoxidase together with its inorganic ion substrates, hydrogen peroxide, and oxidized products is known as the lactoperoxidase system. The lactoperoxidase system plays an important role in the innate immune system by killing bacteria in milk and mucosal (linings of mostly endodermal origin, covered in epithelium, which are involved in absorption and secretion) ...
en] The cloning and sequencing of the rbpa gene coding for a versatile peroxidase from a novel Bjerkandera strain is hereby reported. The 1777 bp isolated fragment contained a 1698 bp peroxidase-encoding gene, interrupted by 11 introns. The 367 amino acid-deduced sequence includes a 27 amino acid-signal peptide. The molecular model, built via homology modelling with crystal structures of four fungal peroxidases, highlighted the amino acid residues putatively involved in manganese binding and aromatic substrate oxidation. The potential heme pocket residues (R44, F47, H48, E79, N85, H177, F194 and D239) include both distal and proximal histidines (H48 and H177). RBP possesses potential calcium-binding residues (D49, G67, D69, S71, S178, D195, T197, I200 and D202) and eight cysteine residues (C3, C15, C16, C35, C121, C250, C286, C316). In addition, RIBP includes residues involved in substrate oxidation: three acidic residues (E37, E41 and D183)-putatively involved in manganese binding and H83 and ...
A picture of substrate binding for small, aromatic substrates at the δ-heme edge emerged largely from chemical modification work (reviewed in [5,6,14,15]), although NMR also played an important role [16-23]. A few structures appeared at the end of the 1990s for horseradish peroxidase and Arthromyces ramosus peroxidase in complex with various small organic molecules [1-3], showing binding at the δ-heme edge, and there is now a collection of other crystal structures in which binding of various organic compounds has been observed at the same site (not all of which are genuine substrates) [1-3,12,13,24-27]. For CcP, there was a general consensus of opinion that small substrates were bound close to the δ-site [7,28-30]. The actual binding site for guaiacol has never been identified, although there is a report of phenol binding to an artificially created cavity in CcP [31].. In our structure, we observed guaiacol binding at two sites, close to Phe68 and Ile40. Guaiacol was not seen bound at the ...
TY - JOUR. T1 - Unfolding and pH studies on manganese peroxidase. T2 - Role of heme and calcium on secondary structure stability. AU - Banci, Lucia. AU - Bartalesi, Ilaria. AU - Ciofi-Baffoni, Simone. AU - Tien, Ming. PY - 2003/1/25. Y1 - 2003/1/25. N2 - The present study characterizes the unfolding and folding processes of recombinant manganese peroxidase. This enzyme contains five disulfide bonds, two calcium ions, and one heme prosthetic group. Circular dichroism in the far UV was used to monitor global changes of the protein secondary structure, whereas UV-visible spectroscopy of the Soret band provided information about local changes in the heme cavity. The effects of reducing agents, oxidizing agents, and denaturants on this process were investigated. In addition to affecting the secondary structure content, these factors also affect the binding of the heme and the calcium ions, both of which have a significant effect on the folding process. Our results also show that denaturants induce ...
Endogenous peroxidase activity has been demonstrated in sections of rat liver fixed briefly by glutaraldehyde perfusion and incubated in Graham and Karnovskys medium for cytochemical demonstration of peroxidase activity (29). In 25-40% of sinusoidal cells, an electron-opaque reaction product is localized in segments of the endoplasmic reticulum, including the perinuclear cisternae, a few Golgi vesicles and saccules and in some large membrane-bounded granules. This staining is abolished after prolonged fixation or boiling of tissue sections in glutaraldehyde, and in the absence of H2O2 or DAB from the incubation medium. Furthermore, the reaction is inhibited completely by sodium azide and high concentrations of H2O2, and partially by KCN and aminotriazole. Among the different cells in hepatic sinusoids, the nonphagocytic fat-storing cells (39) are always peroxidase negative, whereas the lining cells in process of erythrophagocytosis are consistently peroxidase positive. The possible biological ...
The plasma membrane-bound peroxidases (PRX) zmprx01, zmprx66 and zmprx70 and the respiratory burst oxidase homologs (RBOH) rbohA, rbohB, rbohC and rbohD were analysed in this study. The distribution of the genes inside the roots was investigated by real-time qPCR. Therefor four different segments (root tip, elongation zone, differentiation zone and lateral roots) were in focus of the analyses. It could be observed that the genes are differently distributed in the root. The peroxidases were predominantly expressed in the elongation zone and almost not in the root tip. The rboh genes were more inhomogeneous distributed. For each RBOH a specific expression pattern could be detected. rbohA was mostly expressed in the differentiation zone. rbohB was more even expressed in the root. rbohC was even distributed as well but predominantly in the elongation zone. rbohD was mostly expressed in the differentiation zone. For a further investigation of the peroxidases plants were exposed to cadmium (short term and
Reactive oxygen species (ROS), such as O2 and H2O2, play a key role in plant metabolism, cellular signaling, and defense. In leaf cells, the chloroplast is considered to be a focal point of ROS metabolism. It is a major producer of O2 and H2O2 during photosynthesis, and it contains a large array of ROS-scavenging mechanisms that have been extensively studied. By contrast, the function of the cytosolic ROS-scavenging mechanisms of leaf cells is largely unknown. In this study, we demonstrate that in the absence of the cytosolic H2O2-scavenging enzyme ascorbate peroxidase 1 (APX1), the entire chloroplastic H2O2-scavenging system of Arabidopsis thaliana collapses, H2O2 levels increase, and protein oxidation occurs. We further identify specific proteins oxidized in APX1-deficient plants and characterize the signaling events that ensue in knockout-Apx1 plants in response to a moderate level of light stress. Using a dominant-negative approach, we demonstrate that heat shock transcription factors play a ...
A dye-decolorizing peroxidase (DyP) from Pleurotus ostreatus (PosDyP4) catalyzes the oxidation of Mn2+ to Mn3+, in the presence of H2O2, with an efficiency similar to the well-known manganese peroxidases and versatile peroxidases from this and other white-rot fungi. PosDyP4 has been overexpressed in Escherichia coli as an active enzyme, and its crystal structure has been solved at 1.56 Å resolution. A combination of substrate diffusion simulations on the solved structure using the PELE software, electron paramagnetic resonance, and site-directed mutagenesis led to identification of the residues involved in Mn2+ oxidation. The oxidation site in PosDyP4 is different than the conserved site in the other Mn-oxidizing peroxidases mentioned above, and it includes four acidic residues (three aspartates and one glutamate) located at the surface of the protein. Moreover, since the Mn2+ ion is not in direct contact with the heme propionates, a tyrosine residue participates in the electron transfer to the ...
The basidiomycete isolate b19, originally identified by morphological characteristics of the fruiting body as Nematoloma frowardii, efficiently produces manganese peroxidase (MNP) and is used for degradation of natural, persistent aromatic polymers (lignin, humic acids and brown coal components). The N. frowardii MNP has shown good activity in conversion of xenobiotic compounds such as polycyclic hydrocarbons and trinitrotoluene. However, this biotechnologically promising fungus has not previously been studied at the molecular biology level. We show here that according to the molecular characterization of its main MNP isozyme, Nf b19 MNP2, and partial sequencing of its MNP3-, three lignin peroxidase- and two laccase-encoding genes, and the gene encoding the ribosomal SSU 18S RNA, that the fungus has a close phylogenetic relationship to the white-rot basidiomycete Phlebia radiata (Fr.). Ribosomal internal transcribed spacer (ITS) sequence (ITS1+5.8S+ITS2) phylogeny reclassifies Nf b19 as a possible
TY - JOUR. T1 - Metal free MoS2 2D sheets as a peroxidase enzyme and visible-light-induced photocatalyst towards detection and reduction of Cr(vi) ions. AU - Borthakur, Priyakshree. AU - Boruah, Purna K.. AU - Das, Manash R.. AU - Artemkina, Sofya B.. AU - Poltarak, Pavel A.. AU - Fedorov, Vladimir E.. PY - 2018/10/21. Y1 - 2018/10/21. N2 - Two-dimensional molybdenum disulphide (MoS2) sheets were prepared by using simple thermal decomposition method. The synthesized sheets were characterized by different analytical tools including FTIR, XRD, XPS, Raman spectroscopy, HRTEM and FESEM analyses. The synthesized sheets exhibited outstanding peroxidase mimicking activity towards the peroxidase oxidation of chromogenic probe molecule 3,3′,5,5′-tetramethyl benzidine (TMB), producing a blue coloured solution of 3,3′,5,5′-tetramethylbenzidine diimine (TMBDI) in an acidic medium (pH 4). Based on this peroxidase mimicking activity it was utilized for colorimetric determination of toxic Cr(vi) ions ...
In this study, we sought to determine whether the increases in peroxidase activity and electrolyte leakage induced in maize (Zea mays L.) leaves by sodium bisulfite were causally related to the sodium bisulfite-induced increases in sporulation of the pathogen Bipolaris maydis race T on infected maize leaves. Pretreatment of detached leaves of maize inbred W64 A with sodium bisulfite (500 ,ig/ml) for 24 h in the dark at 28°C increased peroxidase activity in the Tms cytoplasm (susceptible) isoline compared with the N cytoplasm (resistant) isoline. No such differences in peroxidase activity between the two isolines were observed when detached leaves were pretreated with double distilled water. The sodium bisulfite-induced increase in peroxidase activity persisted even when leaves pretreated with sodium bisulfite were inoculated with R maydis race T and subsequently incubated for 48 h in the dark at 28° C. Similarly, pretreatment with sodium bisulfite caused a greater increase in electrolyte ...
The ability of plant cells cultivated ,i,in vitro,/i, to metabolize polychlorinated biphenyls (PCBs) was correlated with the morphology of the cultures tested as models for phytoremediation studies. More differentiated cultures showed generally higher transformation capacity. The ability of plant cells to transform PCBs is connected to their viability in the presence of PCBs and their behaviour can be positively correlated with the production of intracellular and extracellular peroxidases. The cultures with high PCB-transforming activity proved to exhibit high peroxidase activity in the presence of PCBs while those with low ability to metabolize PCB showed a decrease of the enzyme activity in the presence of PCBs. Experiments with propylgallate were used to distinguish the ratio of involvement of peroxidases in PCB metabolism. ,p, ...
The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB.
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.
The surface oxidation site (Trp171) in lignin peroxidase (LiP) which is specific for the reaction with the high redox potential substrate, veratryl alcohol (1.4 V), had been previously engineered in a heme peroxidase that has similar protein fold but lacks this activity. The new Trp site was engineered by introducing a Trp residue in the coprinus cinereus peroxidase (CiP) at the equivalent position to that of the Trp171 in LiP. In order to induce the catalytic activity towards veratryl alcohol in CiP, it was also necessary to reproduce the naturally-occurring negatively-charged microenvironment of the Trp site. Multifrequency EPR spectroscopy characterization of the D179W+R258E+R272D variant of CiP unequivocally showed that, upon reaction of the enzyme with hydrogen peroxide a new Trp radical (g-values of gx = 2.0035(5), gy = 2.0027(5) and gz = 2.0022(1)) was formed after the [Fe(IV)=O Por+] intermediate, as a result of intramolecular electron transfer between Trp179 and the porphyrin. The EPR ...
Article Evaluation of manganese peroxidase (MnP) for its ability to resist the ozonization and thereafter decolorize methyl orange. The goal of this study was to determine whether ozone can be used to suppress bacterial growth in operating a white ro...
Suspension culture of compact callus aggregates (CCA) of Daucus carota (carrot), consisting of yellow, spherical cellular clumps displaying tissue differentiation was established. The production of peroxidase which was detected in trace amounts in dispersed carrot cell cultures was found in high amounts (5.0-14.5 U g(-1) FW) in CCA cultures. Kinetics analysis showed that CCA grew quickly for 4-12 days with a specific growth rate of 0.20 day(-1), while the peroxidase activity increased sharply after day 12. Fungal elicitors were found to effectively induce peroxidase synthesis. Peroxidase activity of 54.0 U g(-1) FW was obtained by adding Aspergillus niger elicitor at day 16 to make its concentration in medium 50 mg l(-1). (C) 1998 Elsevier Science B.V. All rights reserved.; Suspension culture of compact callus aggregates (CCA) of Daucus carota (carrot), consisting of yellow, spherical cellular clumps displaying tissue differentiation was established. The production of peroxidase which was ...
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The effect of NaCl on total peroxidase activity, induction of isoperoxidases and lipid peroxidation in 5-day-old seedlings of two contrasting genotypes of Setaria italica L. (Prasad, a salt tolerant cultivar and Lepakshi, a salt susceptible cultivar), was studied. Total peroxidase activity increased under NaCl salinity and the degree of elevation in the activity was salt concentration dependent. Nevertheless, a greater activity was recorded in the tolerant cultivar (cv Prasad) compared to the susceptible (cv Lepakshi) one in all days of sampling. Further, the pattern of isoperoxidases was modified during stress conditions as evident from the electrophoregrams. Although, five acidic isoforms were detected in both cultivars, differences were found between the cultivars. Furthermore, it was observed that acidic isoperoxidases were strongly expressed and an acidic isoperoxidase, A(3p) (27 kDa) is specifically found in the tolerant cultivar (cv Prasad) under NaCl stress. This isoform was partially ...
TY - JOUR. T1 - Structure-function studies of cytochrome c peroxidase from ps. nautica. AU - Alves, T.. AU - Besson, S.. AU - Pereira, A. S.. AU - Pettigrew, G. W.. AU - Moura, José J. G.. AU - Moura, I.. PY - 2001/8. Y1 - 2001/8. M3 - Meeting Abstract. VL - 86. SP - 122. EP - 122. JO - Journal of Inorganic Biochemistry. JF - Journal of Inorganic Biochemistry. SN - 0162-0134. IS - 1. ER - ...
297283835 - EP 0898620 B1 2002-11-06 - ASSAY OF PEROXIDASE ACTIVITY - [origin: WO9739142A1] Chemiluminescent detection of molecules of synthetic or natural origin such as proteins and nucleic acids (DNA and RNA), as well as other biologic molecules, is increasingly replacing radioactive detection as the method of choice where sensitivity is critical. In such assays, luminescence is customarily achieved by the oxidation of a luminol or isoluminol substrate in the presence of an oxidizing agent such as hydrogen peroxide or hydrogen peroxide source, such as perborate, and a peroxidase catalyst such as horseradish peroxidase. To obtain useful levels of luminescence (e.g., detectable levels) by customary techniques, a luminescent enhancer is also present during oxidation. It has been found in the practice of the present invention that azine enhancers have contained an impurity which reduces the properties of the chemiluminescent assay working solutions. The present invention describes a working solution, a
Cold acclimation in woody perennials is a metabolically intensive process, but coincides with environmental conditions that are not conducive to the generation of energy through photosynthesis. While the negative effects of low temperatures on the photosynthetic apparatus during winter have been well studied, less is known about how this is reflected at the level of gene and metabolite expression, nor how the plant generates primary metabolites needed for adaptive processes during autumn. The MapMan tool revealed enrichment of the expression of genes related to mitochondrial function, antioxidant and associated regulatory activity, while changes in metabolite levels over the time course were consistent with the gene expression patterns observed. Genes related to thylakoid function were down-regulated as expected, with the exception of plastid targeted specific antioxidant gene products such as thylakoid-bound ascorbate peroxidase, components of the reactive oxygen species scavenging cycle, and the
Cold acclimation in woody perennials is a metabolically intensive process, but coincides with environmental conditions that are not conducive to the generation of energy through photosynthesis. While the negative effects of low temperatures on the photosynthetic apparatus during winter have been well studied, less is known about how this is reflected at the level of gene and metabolite expression, nor how the plant generates primary metabolites needed for adaptive processes during autumn. The MapMan tool revealed enrichment of the expression of genes related to mitochondrial function, antioxidant and associated regulatory activity, while changes in metabolite levels over the time course were consistent with the gene expression patterns observed. Genes related to thylakoid function were down-regulated as expected, with the exception of plastid targeted specific antioxidant gene products such as thylakoid-bound ascorbate peroxidase, components of the reactive oxygen species scavenging cycle, and the
Catalase-peroxidases are bifunctional enzymes that catalyze the removal of hydrogen peroxide by two distinct pathways (catalase and peroxidase). They are central to antibiotic resistance in Mycobacterium tuberculosis and may be virulence factors in several dangerous human pathogens. These enzymes also hold much promise for engineering new enzymes to combat long-standing problems (e.g., environmental contamination by toxic pollutants). Currently, understanding of catalase-peroxidase structure and function is lacking to facilitate new drug development and enzyme engineering. The purpose of the research described in this dissertation is to understand the molecular basis for the unique catalytic abilities of catalase-peroxidases to fully capitalize on their potential. The versatile catalytic abilities of these enzymes arise from an active site that is normally restricted to one activity (i.e., peroxidase). This is facilitated by three structures which are quite distant from the active site: a ...
peroxidase and IAA oxidase activities. Protein concentration was determined by the method of Bradford (1976) using bovine serum albumin (BSA) as protein standard.. IAA oxidase assay IAA oxidase activity was measured by the spectrophotometric method described by Beffa et al. (1990). Reaction mixtures contained 0.76 ml of 50 mM phosphate buffer (KH2PO4/Na2HPO4, pH 6.0), 0.01 ml of 5 mM MnCl2, 0.01 ml of 5 mM 2,4-dichlorophenol (DCP), 0.02 ml of 14.3 mM IAA and 0.2 ml of enzyme extract. The final volume of the reaction mixtures was 1 ml. Assays were conducted at 25.0 0.5 C for 1 h. Salkowski reagent was used to determine IAA destruction at the wavelength of 535 nm after 1 h. One unit of IAA oxidase activity is equivalent to a DA535 of 1.0 for 1 mg of protein in 1 h. Each value represented the mean of three replicates.. Peroxidase assay Peroxidase (EC 1.11.1.7) activity was determined spectrophotometrically by measuring the increase in absorbance at 470 nm after 30 min incubation at 30.0 0.5 C ...
The report gives the research-based overview of on Global Horseradish Peroxidase (HRP) Market 2019 size, industry status and forecast, competition landscape and growth opportunity. This research report categorizes the global horseradish peroxidase (hrp) market by companies, region, type and end-use industry. It als...
Activity of ascorbate peroxidase (APX) (A), glutathione reductase (GR) (B), dehydroascorbate reductase (DR) (C), and monodehydroascorbate reductase (MR) (D) in
Anti-Human IgG (gamma chain) (Peroxidase Conjugated) Secondary Antibody, Rabbit Polyclonal, Peroxidase (Horseradish) validated in WB, E, IC (ASR3050), Abgent
Anti-Mouse IgG3 (Gamma 3 chain) (Peroxidase Conjugated) Secondary Antibody, Rabbit Polyclonal, Peroxidase (Horseradish) validated in WB, E, IC (ASR3057), Abgent
GPX1 encodes a member of the glutathione peroxidase family, which contains important antioxidant enzymes and helps detoxify hydrogen peroxide with glutathione, and thereby protect cells against oxidative damage (R). It protects the hemoglobin in red blood cells from oxidative breakdown.. H2O2 is also essential for growth-factor mediated signal transduction, mitochondrial function, and maintenance of antioxidant balance; therefore, by limiting H2O2 accumulation, glutathione peroxidases are also involved in modulating these processes.. It is also a selenoprotein, requiring selenium to function.. Associated with risk for breast cancer (R). ...
... catalyzed by the presence of a peroxidase (such as horseradish peroxidase). The hydrogen peroxide is itself produced by an ... p. 9. ISBN 9788176487740.CS1 maint: multiple names: authors list (link) Carlos G. Dosoretz; Gary Ward (2006). "Peroxidases". In ...
Banerjee, R. K.; Bose, A. K.; Chakraborty, T. K.; De, S. K.; Datta, A. G. (1985). "Peroxidase-catalysed iodotyrosine formation ... Banerjee, R. K.; Datta, A. G. (1986). "Salivary peroxidases". Mol Cell Biochem. 70 (1): 21-29. doi:10.1007/bf00233801. PMID ... lysozyme and peroxidase. It has not been shown that human licking of wounds disinfects them, but licking is likely to help ...
Dunford, BH (1999). Heme peroxidases. John Wiley. ISBN 0-471-24244-6. Dawson, J. (22 April 1988). "Probing structure-function ... relations in heme-containing oxygenases and peroxidases". Science. 240 (4851): 433-439. doi:10.1126/science.3358128. PMID ...
Enzyme kinetics Glutathione peroxidase Peroxidase Superoxide dismutase Chelikani P, Fita I, Loewen PC (January 2004). " ... "PeroxiBase - The peroxidase database". Swiss Institute of Bioinformatics. Archived from the original on 2008-10-13. Retrieved ... Maehly AC, Chance B (1954). "The assay of catalases and peroxidases". Methods of Biochemical Analysis. Methods of Biochemical ... contains catalases and peroxidases. To activate the noxious spray, the beetle mixes the contents of the two compartments, ...
... produces chitinase and peroxidase. Wang, W; Mai-Gisondi, G; Stogios, PJ; Kaur, A; Xu, X; Cui, H; ... Iqbal, M.; Mercer, D. K.; Miller, P. G. G.; McCarthy, A. J. (1 June 1994). "Thermostable extracellular peroxidases from ...
Rhee SG, Kang SW, Chang TS, Jeong W, Kim K (July 2001). "Peroxiredoxin, a novel family of peroxidases". IUBMB Life. 52 (1-2): ... Catalase Oxidative stress Peroxidase Peroxiredoxin classification index Reactive oxygen species Superoxide dismutase Rhee S, ... Other names include thiol specific antioxidant (TSA) and thioredoxin peroxidase (TPx). Mammals express six peroxiredoxins: 1- ... Poole LB (January 2005). "Bacterial defenses against oxidants: mechanistic features of cysteine-based peroxidases and their ...
... thyroid peroxidase activity; and hormone release. Graves' disease GRCh38: Ensembl release 89: ENSG00000165409 - Ensembl, May ...
ISBN 978-1-55407-651-2. Singh, R.; Eltis, L.D. (2015). "The multihued palette of dye-decolorizing peroxidases". Archives of ... "Lignin-degrading peroxidases in Polyporales: an evolutionary survey based on 10 sequenced genomes". Mycologia. 105 (6): 1428- ...
There are at least four different glutathione peroxidase isozymes in animals. Glutathione peroxidase 1 is the most abundant and ... Glutathione peroxidase is an enzyme containing four selenium-cofactors that catalyzes the breakdown of hydrogen peroxide and ... Surprisingly, glutathione peroxidase 1 is dispensable, as mice lacking this enzyme have normal lifespans, but they are ... In addition to its direct antioxidant effects, ascorbic acid is also a substrate for the redox enzyme ascorbate peroxidase, a ...
It was first limited to class III peroxidases (plant peroxidases) and was then expanded to include all possible haem and non- ... The majority of haem and non-haem peroxidase sequences can now be found in the PeroxiBase. The database is hosted by the Swiss ... Peroxidase sequences come from other general public databases (NCBI, TIGR, UniProt KnowledgeBase: all the databases used are ... haem peroxidase protein sequences. Many researchers and bioinformaticians from the University of Geneva joined their efforts to ...
PRSS7 Eosinophil peroxidase deficiency; 261500; EPX Epidermodysplasia verruciformis; 226400; TMC6 Epidermodysplasia ...
... eosinophil peroxidase (EPO), thyroid peroxidase (TPO), and prostaglandin H synthase (PGHS). A heme cofactor is bound near the ... Lactoperoxidase is a peroxidase enzyme secreted from mammary, salivary, and other mucosal glands that functions as a natural ... Lactoperoxidase is a member of the heme peroxidase family of enzymes. In humans, lactoperoxidase is encoded by the LPO gene. ... Peroxidase-generated hypothiocyanite inhibits herpes simplex virus and human immunodeficiency virus. Both the hypothiocyanite ...
These systems include peroxidases, catalases, and superoxide dismutases. A complementary metalloprotein to those that react ...
ISBN 978-1-947988-83-5. India portal Biology portal Medicine portal Lactoferrin Haem peroxidase Lactoperoxidase Please see ... "Structural basis of activation of mammalian heme peroxidases". Progress in Biophysics and Molecular Biology. 133: 49-55. doi: ...
Anti-thyroid peroxidase (anti-TPO) antibodies are specific for the autoantigen TPO, a 105kDa glycoprotein that catalyses iodine ... McLachlan SM, Rapoport B (2000). "Autoimmune response to the thyroid in humans: thyroid peroxidase--the common autoantigenic ... The most clinically relevant anti-thyroid autoantibodies are anti-thyroid peroxidase antibodies (anti-TPO antibodies, TPOAb), ... Taurog A (May 1999). "Molecular evolution of thyroid peroxidase". Biochimie. 81 (5): 557-62. doi:10.1016/S0300-9084(99)80110-2 ...
Glutathione peroxidase activity of inorganic selenium and seleno-DL-cysteine. Experientia 31: 769-770, 1975 Beutler E, et al. ... Electrophoretic polymorphism of glutathione peroxidase. Ann Hum Genet 38: 163-169, 1974 Beutler E, et al. ...
The enzyme is thyroid peroxidase. The small amount of T3 could be important because different tissues have different ...
... l is one important characteristic of animal peroxidases; plant peroxidases incorporate heme B. Lactoperoxidase and ... In peroxidase reactions, the porphyrin molecule also serves as an electron source, being able to delocalize radical electrons ... Heme m contains the two ester bonds at the heme 1- and 5-methyl groups also present in heme l of other mammalian peroxidases, ... In general, diatomic gases only bind to the reduced heme, as ferrous Fe(II) while most peroxidases cycle between Fe(III) and Fe ...
Some bacteria have NADH-dependent peroxidases specific for H2O2. The main nonenzymatic antioxidants in E. coli are GSH and ...
White WE, Pruitt KM, Mansson-Rahemtulla B (February 1983). "Peroxidase-Thiocyanate-Peroxide Antibacterial System Does Not ... Hypothiocyanite is formed by peroxidase catalysis of hydrogen peroxide and thiocyanate: H2O2 + SCN− → OSCN− + H2O ... "Active site structure and catalytic mechanisms of human peroxidases". Arch. Biochem. Biophys. 445 (2): 199-213. doi:10.1016/j. ... respiratory syncytial virus and echovirus type 11 by peroxidase-generated hypothiocyanite". Antiviral Res. 26 (2): 161-71. doi: ...
Here, trypanothione-dependent enzymes such as tryparedoxin peroxidase (TryP) reduce peroxides using electrons donated either ... Trypanothione-dependent enzymes include reductases, peroxidases, glyoxalases and transferases. Trypanothione-disulfide ...
"New and classic families of secreted fungal heme peroxidases". Appl Microbiol Biotechnol. 87 (3): 871-897. doi:10.1007/s00253- ...
Norisoprenoids, such as C13-norisoprenoids found in grape (Vitis vinifera) or wine, can be produced by fungal peroxidases or ... "Generation of Norisoprenoid Flavors from Carotenoids by Fungal Peroxidases". Journal of Agricultural and Food Chemistry. 57 (21 ...
... can be produced by fungal peroxidases or glycosidases. While terpenes and terpenoids occur widely, their extraction from ... "Generation of Norisoprenoid Flavors from Carotenoids by Fungal Peroxidases". Journal of Agricultural and Food Chemistry. 57 (21 ...
Glutathione peroxidase 3 (GPx-3), also known as plasma glutathione peroxidase (GPx-P) or extracellular glutathione peroxidase ... Lin JC, Kuo WR, Chiang FY, Hsiao PJ, Lee KW, Wu CW, Juo SH (May 2009). "Glutathione peroxidase 3 gene polymorphisms and risk of ... "Entrez Gene: glutathione peroxidase 3 (plasma)". Takahashi K, Avissar N, Whitin J, Cohen H (August 1987). "Purification and ... Saga Y, Ohwada M, Suzuki M, Konno R, Kigawa J, Ueno S, Mano H (December 2008). "Glutathione peroxidase 3 is a candidate ...
Glutathione peroxidase 7 is an enzyme that in humans is encoded by the GPX7 gene. GRCh38: Ensembl release 89: ENSG00000116157 ... PDBe-KB provides an overview of all the structure information available in the PDB for Human Glutathione peroxidase 7 Biology ... "Entrez Gene: GPX7 glutathione peroxidase 7". Opalenik SR, Ding Q, Mallery SR, Thompson JA (1998). "Glutathione depletion ... 2001). "Human immunodeficiency virus type 1 Tat protein impairs selenoglutathione peroxidase expression and activity by a ...
Glutathione peroxidase 8 (GPx-8) is an enzyme that in humans is encoded by the GPX8 gene. GPx-8 is a member of the glutathione ... "Entrez Gene: glutathione peroxidase 8 (putative)". Toppo S, Vanin S, Bosello V, Tosatto SC (September 2008). "Evolutionary and ... PDBe-KB provides an overview of all the structure information available in the PDB for Human Glutathione Peroxidase 8 (GPX8) ... structural insights into the multifaceted glutathione peroxidase (Gpx) superfamily". Antioxid. Redox Signal. 10 (9): 1501-14. ...
An ultrastructural and peroxidase-cytochemical study". Lab Invest. 43 (2): 172-81. PMID 7401631. De Vos R, De Wolf-Peeters C, ...
It is formed by eosinophil peroxidase. Kambayashi Y, Ogino K, Takemoto K, et al. (January 2009). "Preparation and ...
Glutathione peroxidase 6 (GPx-6) is an enzyme that in humans is encoded by the GPX6 gene. This gene product belongs to the ... "Entrez Gene: glutathione peroxidase 6 (olfactory)". Kryukov GV, Castellano S, Novoselov SV, Lobanov AV, Zehtab O, Guigó R, ... 2001). "Human immunodeficiency virus type 1 Tat protein impairs selenoglutathione peroxidase expression and activity by a ... glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) ...
Peroxidases,state=autocollapse}} *shows the template collapsed to the title bar if there is a {{navbar}}, a {{sidebar}}, or ... Peroxidases,state=collapsed}} to show the template collapsed, i.e., hidden apart from its title bar ... Peroxidases,state=expanded}} to show the template expanded, i.e., fully visible ... Retrieved from "https://en.wikipedia.org/w/index.php?title=Template:Peroxidases&oldid=704356592" ...
Glutathione peroxidase (GPx) (EC 1.11.1.9) is the general name of an enzyme family with peroxidase activity whose main ... glutathione peroxidase 4 (phospholipid hydroperoxidase) GPX5. Chr. 6 p21.32. glutathione peroxidase 5 (epididymal androgen- ... Glutathione peroxidase 2 is an intestinal and extracellular enzyme, while glutathione peroxidase 3 is extracellular, especially ... Glutathione peroxidase was discovered in 1957 by Gordon C. Mills.[8] Clinical significance[edit]. It has been shown that low ...
Inhibition of Peroxidase Activity of Cytochrome c: De Novo Compound Discovery and Validation. Ahmet Bakan, Alexandr A. Kapralov ... Inhibition of Cytochrome c Peroxidase Activity. Ahmet Bakan, Alexandr A. Kapralov, Hulya Bayir, Feizhou Hu, Valerian E. Kagan ... Inhibition of Cytochrome c Peroxidase Activity. Ahmet Bakan, Alexandr A. Kapralov, Hulya Bayir, Feizhou Hu, Valerian E. Kagan ... Pore opening is enhanced by peroxidase activity of cyt c gained upon its complexation with cardiolipin in the presence of ...
Animal heme-dependent peroxidases is a family of peroxidases. Peroxidases are found in bacteria, fungi, plants and animals. On ... eosinophil peroxidase (EPO); lactoperoxidase (LPO); thyroid peroxidase (TPO); prostaglandin H synthase (PGHS); and peroxidasin ... The peroxidase active site, which catalyzes the reduction of PGG2 to PGH2, is located on the other side of the molecule, at the ... Kimura S, Hong YS, Kotani T, Ohtaki S, Kikkawa F (1989). "Structure of the human thyroid peroxidase gene: comparison and ...
Ascorbate peroxidase Chloride peroxidase Cytochrome c peroxidase Haloperoxidase Hemoprotein Immunoperoxidase Lactoperoxidase ... Haem-using haem peroxidase and the related animal heme-dependent peroxidases DyP-type peroxidase family Catalase some ... Peroxidases are sometimes used as histological markers. Cytochrome c peroxidase is used as a soluble, easily purified model for ... Peroxidases typically catalyze a reaction of the form: ROOR ′ + 2 e − electron donor + 2 H + → Peroxidase ROH + R ′ OH {\ ...
Eosinophil peroxidase deficiency is a condition that affects certain white blood cells called eosinophils but causes no health ... In eosinophil peroxidase deficiency, eosinophils have little or no eosinophil peroxidase. A lack of this protein does not seem ... Eosinophil peroxidase deficiency is estimated to occur in 8.6 in 1,000 Yemenite Jews, in 3 in 1,000 North-African Jews, and in ... Mutations in the EPX gene cause eosinophil peroxidase deficiency. The EPX gene provides instructions for making the eosinophil ...
The thyroid peroxidase antibodies test is primarily used to help diagnose and monitor autoimmune conditions involving the ... A thyroid peroxidase antibodies test checks the levels of antibodies made against the compound thyroid peroxidase (TPO) in the ... Thyroid peroxidase is an enzyme produced by the thyroid gland. The thyroid is a small, butterfly-shaped gland in the neck that ... The thyroid peroxidase antibodies test is considered a safe procedure. However, as with many medical tests, some problems can ...
Included is a discussion of peroxidases that also act as catalases and oxygenases. Heme Peroxidases serves as an essential text ... Discussed functions of peroxidases range from cell wall synthesis, synthesis of prostaglandins, role in drug suppression of ... Chapters written and edited by worldwide experts span a range of heme peroxidases from plants, yeast, bacteria and mammals. ... This book provides a comprehensive single source of information on the various aspects of heme peroxidase structure, function ...
Learn about the thyroid peroxidase test, used to diagnose thyroid disorders, as well as other autoimmune conditions and ... Thyroid peroxidase test is a test that measures the level of an antibody that is directed against thyroid peroxidase (TPO). ... Thyroid peroxidase (TPO) is an enzyme made in the thyroid gland that is important in the production of thyroid hormone. TPO is ... Autoantibodies to thyroid peroxidase (TPOAb) are produced within the body. The presence of TPOAb in the blood reflects a prior ...
Supplementary Figure 3: Comparison of peroxidase staining conditions.. From: Multiplexed peroxidase-based electron microscopy ... Supplementary Figure 3: Comparison of peroxidase staining conditions. , Nature Neuroscience. ...
IPR010255 Haem_peroxidase_sf. IPR000823 Peroxidase_pln. IPR019794 Peroxidases_AS. IPR019793 Peroxidases_heam-ligand_BS. ... IPR010255 Haem_peroxidase_sf. IPR000823 Peroxidase_pln. IPR019794 Peroxidases_AS. IPR019793 Peroxidases_heam-ligand_BS. ... PS00435 PEROXIDASE_1, 1 hit. PS00436 PEROXIDASE_2, 1 hit. PS50873 PEROXIDASE_4, 1 hit. ... PS00435 PEROXIDASE_1, 1 hit. PS00436 PEROXIDASE_2, 1 hit. PS50873 PEROXIDASE_4, 1 hit. ...
... ,ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative ... TPO (Ab) EIA Thyroid Function 012-MDKAM-1 Thyroid Peroxidase. 7. TG (Ab) RIA Thyroid Function 014-HD 27.1 Thyroglobulin. 8. ... TPO (Ab) RIA Thyroid Function 014-HD-90.1 Thyroid Peroxidase. 4. TG (Ab) EIA Thyroid Function 012-MDKAT-1 Thyroglobulin anti-tg ...
Oxidoreductase, PeroxidaseUniRule annotation. ,p>Information which has been generated by the UniProtKB automatic annotation ... Thioredoxin-dependent peroxidase TPX1/2 (Fragment). Toxoplasma gondii GAB2-2007-GAL-DOM2 ... Belongs to the glutathione peroxidase family.UniRule annotation. Automatic assertion according to rulesi ... tr,Q6GVI1,Q6GVI1_TOXGO Glutathione peroxidase OS=Toxoplasma gondii OX=5811 PE=2 SV=1 ...
... manganese peroxidase, versatile peroxidase and generic peroxidase), dye-decolorizing peroxidases, and heme-thiolate peroxidases ... They comprise three major groups: class II peroxidases (including lignin peroxidase, ... Manganese peroxidases and unspecific peroxygenases were found expressed in all nine investigated forest sites, and dye- ... Using this information, we were able to match, in environmental litter samples, two manganese peroxidase sequences to Mycena ...
glutathione peroxidase 1. Names. cellular glutathione peroxidase. selenium-dependent glutathione peroxidase 1. NP_032186.2. *EC ... GSH_Peroxidase; Glutathione (GSH) peroxidase family; tetrameric selenoenzymes that catalyze the reduction of a variety of ... Gpx1 glutathione peroxidase 1 [Mus musculus] Gpx1 glutathione peroxidase 1 [Mus musculus]. Gene ID:14775 ... glutathione peroxidase 1provided by MGI. Primary source. MGI:MGI:104887 See related. Ensembl:ENSMUSG00000063856 Vega: ...
Pentacoordination of the heme iron of Arthromyces ramosus peroxidase shown by a 1.8 A resolution crystallographic study at pH ... PEROXIDASE protein, length: 344 (BLAST) Sequence Similarity Cutoff. Rank. Chains in Cluster. Cluster ID / Name. Structural ...
Binding of iodide to Arthromyces ramosus peroxidase investigated with X-ray crystallographic analysis, 1H and 127I NMR ... Description: PEROXIDASE protein , Length: 344 No structure alignment results are available for 1GZA.A explicitly.. It is ...
Horseradish peroxidase: a tool for study of the neuroendocrine cell and other peptide-secreting cells. Methods Enzymol. 1983; ... Neuronal transport of acid hydrolases and peroxidase within the lysosomal system or organelles: involvement of agranular ...
Sigma-Aldrichs peroxidase product is recognized around the world as the industry standard for diagnostic manufacturing and ... Peroxidase Enzyme Products , Peroxidase Substrates , Peroxidase Inhibitors. For decades Sigma-Aldrich has manufactured ... Sigma Peroxidase. From Horseradish and Soybean. EC 1.11.1.7. Synonyms: Hydrogen peroxide oxidoreductase, HRP. Peroxidase ... For conjugating proteins such as antibodies to peroxidase, choose a peroxidase with an RZ value of at least 3.0.. ...
... non-selenium glutathione peroxidase activity, reduced glutathione peroxidase activity, selenium-glutathione peroxidase activity ... Gene Ontology Term: glutathione peroxidase activity. GO ID. GO:0004602 Aspect. Molecular Function. Description. Catalysis of ... glutathione:hydrogen-peroxide oxidoreductase activity, GSH peroxidase activity, ...
Glutathione peroxidase 4 prevents necroptosis in mouse erythroid precursors.. Canli Ö1, Alankuş YB2, Grootjans S3, Vegi N4, ... The antioxidant enzyme glutathione peroxidase 4 (Gpx4) is a key regulator of oxidative stress-induced cell death. We show that ...
... and hemin are attracting a lot of interest due to their peroxidase activity mimicking the natural enzyme horseradish peroxidase ... Peroxidase mimicking DNAZymes degrade graphene oxide R. Kurapati and A. Bianco, Nanoscale, 2018, Accepted Manuscript , DOI: ... The current study suggests that peroxidase activity of DNAZymes is similar to HRP and it is able to degrade carbon-based ... DNAZymes made of supramolecular guanine-rich G-quadruplexes and hemin are attracting a lot of interest due to their peroxidase ...
Find thyroid peroxidase information, treatments for thyroid peroxidase and thyroid peroxidase symptoms. ... MedHelps thyroid peroxidase Center for Information, Symptoms, Resources, Treatments and Tools for thyroid peroxidase. ... Posts on thyroid peroxidase (30). I got my thyroid peroxidase and is 169, is that consider Hashimostos, - Thyroid Disorders ... Thyroid Peroxidase Ab HIGH but T3 & T4 Normal High RBC COUNT! HELP Me PLEAE! - Thyroid Disorders Community ...
Porphobilinogen oxygenase and horseradish peroxidase show dual oxygenase and peroxidase activities. By treating porphobilinogen ... When horseradish peroxidase was treated in the same manner only the peroxidase activity was lost while its oxygenase activity ... The dual oxygenase and peroxidase activities of porphobilinogen oxygenase and horseradish peroxidase: a study using the ... Porphobilinogen oxygenase and horseradish peroxidase show dual oxygenase and peroxidase activities. By treating porphobilinogen ...
Glutathione peroxidase activity protects against symptoms of Huntingtons disease. *Download PDF Copy ... Their paper, Glutathione peroxidase activity is neuroprotective in models of Huntingtons disease, was published in Nature ... The team now aim to further validate the observations regarding glutathione peroxidase activity, in order to understand whether ... The team of University of Leicester researchers identified that glutathione peroxidase activity - a key antioxidant in cells - ...
Thyroid peroxidase antibodies are molecules that occur when the body attacks certain parts of its own thyroid gland. They ... Having thyroid peroxidase antibodies is also associated with a number of other conditions. Patients with Graves disease, an ... In general, the presence of thyroid peroxidase antibodies in the blood is an abnormal finding. Antibodies are proteins made by ... Some normal, asymptomatic people can have detectable levels of thyroid peroxidase antibodies. As much as 5 to 10 percent of the ...
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... Article Translations: (Spanish). What It Is. A thyroid peroxidase antibodies test ... Thyroid peroxidase is an enzyme produced by the thyroid gland. The thyroid is a small, butterfly-shaped gland in the neck that ... The thyroid peroxidase antibodies test is considered a safe procedure. However, as with many medical tests, some problems can ... The thyroid peroxidase antibodies test is primarily used to help diagnose and monitor autoimmune conditions involving the ...
The 29-Kilodalton Thiol-Dependent Peroxidase of Entamoeba histolytica Is a Factor Involved in Pathogenesis and Survival of the ... Novel Promoter Sequence Required for Manganese Regulation of Manganese Peroxidase Isozyme 1 Gene Expression in Phanerochaete ... Overexpression of Mitochondrial Leishmania major Ascorbate Peroxidase Enhances Tolerance to Oxidative Stress-Induced Programmed ...
  • For example, horseradish peroxidase can use a variety of organic compounds as electron donors and acceptors. (wikipedia.org)
  • Horseradish peroxidase has an accessible active site, and many compounds can reach the site of the reaction. (wikipedia.org)
  • For example, phenols, which are important pollutants, can be removed by enzyme-catalyzed polymerization using horseradish peroxidase. (wikipedia.org)
  • Horseradish peroxidase (HRP) is isolated from horseradish roots ( Amoracia rusticana ) and belongs to the ferroprotoporphyrin group of peroxidases. (sigmaaldrich.com)
  • Horseradish peroxidase is widely used as a label for immunoglobulins in many different immunochemistry applications including ELISA, immunoblotting and immunohistochemistry. (sigmaaldrich.com)
  • DNAZymes made of supramolecular guanine-rich G-quadruplexes and hemin are attracting a lot of interest due to their peroxidase activity mimicking the natural enzyme horseradish peroxidase (HRP). (rsc.org)
  • Horseradish peroxidase (HRP) immobilized on colloidal gold and then deposited on an electrode surface can be reduced at a convenient rate at low voltage (Ag/AgCl) without an electron transfer mediator. (google.com)
  • 2. The biooelectrode of claim 1 wherein the peroxidase is horseradish peroxidase. (google.com)
  • 9. A bioelectrode for mediatorless electrochemical detection of glucose comprising a conducting surface having a first deposited layer of monolayer-coated colloidal gold-adsorbed horseradish peroxidase and a second deposited layer of colloidal gold-adsorbed glucose oxidase atop said first layer. (google.com)
  • 11. The bioelectrode of claim 9 wherein the first layer of colloidal gold-adsorbed horseradish peroxidase is evaporatively deposited onto the electrode surface prior to evaporative deposition of the second layer of colloidal gold-adsorbed glucose oxidase. (google.com)
  • In particular, mediatorless detection of glucose is possible with colloidal gold adsorbed horseradish peroxidase in the presence of glucose oxidase. (google.com)
  • Product is a conjugate of Protein A and highly purified (Rz >3.0) horseradish peroxidase. (mpbio.com)
  • The effect of organic solvents on horseradish peroxidase structure and function has been studied. (nih.gov)
  • Antibodies specific for horseradish peroxidase (HRPeroxase) bind to neuronal membranes in Drosophila and serve as a specific neuronal marker. (pnas.org)
  • Electronic spectra observed prior to compound I formation during the reaction of H2O2 with both wild type and the R38L mutant of horseradish peroxidase C (HRP-C) have been attributed to this intermediate. (rti.org)
  • Alternatively, anti-horseradish peroxidase conjugated with other enzymes or fluorophores may be used to enhance signal and reduce background, since the final signal does not depend on the enzyme activity of peroxidase, but on the antigenicity of horseradish peroxidase. (jacksonimmuno.com)
  • TMR's report on the global horseradish peroxidase market studies past as well as current growth trends and opportunities to gain valuable insights of these indicators of the market during the forecast period from 2020 to 2030. (bccresearch.com)
  • The report provides revenue of the global horseradish peroxidase market for the period 2018-2030, considering 2019 as the base year and 2030 as the forecast year. (bccresearch.com)
  • The report also provides the compound annual growth rate (CAGR) for the global horseradish peroxidase market during the forecast period. (bccresearch.com)
  • Extensive secondary research involved referring to key players' product literature, annual reports, press releases, and relevant documents to understand the global horseradish peroxidase market. (bccresearch.com)
  • Analysts have employed a combination of top-down and bottom-up approaches to study various phenomenon in the global horseradish peroxidase market. (bccresearch.com)
  • Furthermore, the report sheds light on the changing competitive dynamics in the global horseradish peroxidase market. (bccresearch.com)
  • These indices serve as valuable tools for existing market players as well as for entities interested in entering the global horseradish peroxidase market. (bccresearch.com)
  • The report delves into the competition landscape of the global horseradish peroxidase market. (bccresearch.com)
  • Key players operating in the global horseradish peroxidase market have been identified, and each one of these has been profiled for distinguishing business attributes. (bccresearch.com)
  • Company overview, financial standings, recent developments, and SWOT are some of the attributes of players in the global horseradish peroxidase market that have been profiled in this report. (bccresearch.com)
  • What is the scope of growth of product companies in the global horseradish peroxidase market? (bccresearch.com)
  • What will be the Y-o-Y growth of the global horseradish peroxidase market between 2020 and 2030? (bccresearch.com)
  • What is the influence of changing trends in life science research on the global horseradish peroxidase market? (bccresearch.com)
  • Will North America continue to be the most profitable market for horseradish peroxidase providers? (bccresearch.com)
  • Which factors are anticipated to hamper the growth of the global horseradish peroxidase market during the forecast period? (bccresearch.com)
  • Which are the leading companies in the global horseradish peroxidase market? (bccresearch.com)
  • A unique research methodology has been utilized by TMR to conduct comprehensive research on the growth of the global horseradish peroxidase market and arrive at conclusions on its growth prospects. (bccresearch.com)
  • Secondary sources referred to by analysts during the production of the global horseradish peroxidase market report include statistics from company annual reports, SEC filings, company websites, investor presentations, regulatory databases, government publications, and industry white papers. (bccresearch.com)
  • Analysts have also interviewed senior managers, product portfolio managers, and market intelligence managers who contributed to the production of TMR's study on the horseradish peroxidase market as primary research sources. (bccresearch.com)
  • These primary and secondary research sources have provided exclusive information during interviews, which serves as a validation from the horseradish peroxidase market leaders. (bccresearch.com)
  • Access to an extensive internal repository and external proprietary databases enabled this report to address specific details and questions about the global horseradish peroxidase market with accuracy. (bccresearch.com)
  • This has helped in reaching TMR's estimates on future prospects of the global horseradish peroxidase market more reliably and accurately. (bccresearch.com)
  • The activity assay demonstrated that [email protected] exhibited an activity similar to that of horseradish peroxidase, but with a much higher activity. (mdpi.com)
  • With lactoperoxidase and horseradish peroxidase, all the tested thiols were inactive or poorly active as peroxidase-oxidase substrates. (biochemj.org)
  • Peroxidases or peroxide reductases (EC number 1.11.1.x) are a large group of enzymes which play a role in various biological processes. (wikipedia.org)
  • ce {Peroxidase}}]{ROH}+R'OH}}} For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides. (wikipedia.org)
  • 12. The bioelectrode of claim 1 further comprising depositing at least two oxidase enzymes atop the deposited monolayer-coated colloidal gold peroxidase layer. (google.com)
  • 1990) peroxidase activity increases with ripening.The activity of bound and soluble enzymes isolated from the pulp of ripening banana fruit were increased at the onset of the respiration climateric (Haard, 1973). (scielo.br)
  • In contrast, the effects of these additives on thermal behaviour of the heat resistant enzymes related to off-flavors generation, as peroxidase and polyphenoloxidase, need to have better understanding. (scielo.br)
  • The Glutathione Peroxidase (GPx, EC 1.11.1.9) family of enzymes plays and important role in the protection of organisms from oxidative damage. (abcam.com)
  • High amounts of malic, citric, tartaric, oxalic and other organic acids combined with the enzymes catalase and peroxidase give honey its renowned antibacterial properties. (wordnik.com)
  • Peroxidase enzymes are widely distributed in plants and animals, including bacteria, to protect cells against the effects of oxidative stress and cell damage due to hydrogen peroxide. (flinnsci.com)
  • In recent years, the synergistic relationship between NADPH oxidase (NOX)/dual oxidase (DUOX) enzymes and peroxidases has received increased attention. (genetics.org)
  • These enzymes generate the H 2 O 2 required as substrate for the peroxidases. (genetics.org)
  • Among the thousands of different enzymes in a single cell, peroxidases are among the most active and the most widely distributed. (flinnsci.com)
  • Glutathione peroxidase serves in the detoxification of hydrogen peroxide, and is one of the most vital antioxidant enzymes in humans. (prospecbio.com)
  • A thyroid peroxidase antibodies test checks the levels of antibodies made against the compound thyroid peroxidase (TPO) in the bloodstream. (kidshealth.org)
  • Ordinarily, a healthy immune system doesn't make significant levels of antibodies against thyroid peroxidase, because it's not "foreign," but rather a necessary component of thyroid tissue. (kidshealth.org)
  • The thyroid peroxidase antibodies test is primarily used to help diagnose and monitor autoimmune conditions involving the thyroid gland, such as Hashimoto's thyroiditis and Graves disease. (kidshealth.org)
  • The thyroid peroxidase antibodies test is considered a safe procedure. (kidshealth.org)
  • If you have questions about the thyroid peroxidase antibodies test, speak with your doctor. (kidshealth.org)
  • My thyroid peroxidase antibodies level is 360 and my thyroglobulin antibodies level is 81 o. (medhelp.org)
  • What Are Thyroid Peroxidase Antibodies? (wisegeek.com)
  • When the body attacks certain parts of its own thyroid gland, evidence of this can be found in the presence of abnormal molecules called thyroid peroxidase (TPO) antibodies . (wisegeek.com)
  • In general, the presence of thyroid peroxidase antibodies in the blood is an abnormal finding. (wisegeek.com)
  • With production of the TPO antibodies, the body is making a protein that attacks a molecule important to the function of the thyroid, the thyroid peroxidase enzyme. (wisegeek.com)
  • A positive result for the presence of thyroid peroxidase antibodies is most often found in autoimmune thyroiditis, a condition also known as Hashimoto's thyroiditis. (wisegeek.com)
  • Having thyroid peroxidase antibodies is also associated with a number of other conditions. (wisegeek.com)
  • Patients with Graves' disease, an autoimmune thyroid disorder that causes the thyroid to overproduce the thyroid hormone, can have thyroid peroxidase antibodies present in their blood. (wisegeek.com)
  • Some normal, asymptomatic people can have detectable levels of thyroid peroxidase antibodies. (wisegeek.com)
  • She is fatigued, and a blood test revealed a thyroid peroxidase antibodies level of 587 IU/mL. (mdedge.com)
  • The short answer is: No. Thyroid peroxidase (TPO) antibodies are a marker for the presence of autoimmune thyroid disease. (mdedge.com)
  • a peroxidases isolated from horseradish that is used in immunohistochemistry to label the antigen-antibody complex. (drugs.com)
  • Thyroid peroxidase test is a test that measures the level of an antibody that is directed against thyroid peroxidase (TPO). (rxlist.com)
  • Mohamed A. Adlan and Lakdasa D. Premawardhana, "Thyroid Peroxidase Antibody and Screening for Postpartum Thyroid Dysfunction," Journal of Thyroid Research , vol. 2011, Article ID 745135, 5 pages, 2011. (hindawi.com)
  • Cytiva Lifescience™ anti-Mouse IgG, peroxidase-linked species-specific whole antibody (from sheep) Secondary Antibody is designed for applications such as protein blotting, ELISA and immunocytochemistry. (fishersci.com)
  • Peroxidase can be conjugated to a secondary antibody in order to yield a colored fluorescent end product. (prosci-inc.com)
  • TMB ELISA Peroxidase Substrate (3, 3', 5, 5' - Tetramethylbenzidine) is a chromogenic substrate used to visualize antibody reactivity in ELISA experiments. (rockland-inc.com)
  • By using tissue-specific skpo-1 RNAi and immunohistochemical localization with an anti- SKPO-1 antibody, it was determined that the peroxidase is functionally and physically present in the hypodermis. (genetics.org)
  • Glutathione peroxidase 1 (GPx1) is the most abundant version, found in the cytoplasm of nearly all mammalian tissues, whose preferred substrate is hydrogen peroxide. (wikipedia.org)
  • The Enzyme Explorer's Substrate Index provides links to several chromogenic and chemiluminescent hydrogen donors used to assay peroxidase activity. (sigmaaldrich.com)
  • The ImmPACT DAB Peroxidase (HRP) Substrate reacts with HRP to yield an insoluble brown-colored product. (vectorlabs.com)
  • The activity of this novel peroxidase was determined using guaiacol as its substrate. (magiran.com)
  • TMB ELISA Peroxidase Substrate is ideal for investigators in Microbiology, Immunology, and Cancer research. (rockland-inc.com)
  • TMB ELISA Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. (rockland-inc.com)
  • TMB ELISA Peroxidase Substrate incubation time will vary depending on the assay conditions. (rockland-inc.com)
  • No dilutions are required, TMB ELISA Peroxidase Substrate comes ready to use. (rockland-inc.com)
  • The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. (nih.gov)
  • therefore, by limiting H2O2 accumulation, glutathione peroxidases are also involved in modulating these processes. (nih.gov)
  • Haemoglobin-induced oxidative stress is associated with both endogenous peroxidase activity and H2O2 generation from polyunsaturated fatty acids," Free Radical Research , vol. 45, no. 3, pp. 303-316, 2011. (hindawi.com)
  • Flavonoid- peroxidase reaction as a detoxification mechanism of plants against H2O2. (wordnik.com)
  • Ascorbate peroxidases are haem proteins that efficiently scavenge H2O2 in the cytosol and chloroplasts of plants. (semanticscholar.org)
  • Glutathione peroxidases use glutathione as an electron donor and are active with both hydrogen peroxide and organic hydroperoxide substrates. (wikipedia.org)
  • A total of 22 isoenzymes including allelic variants were successfully expressed in Pichia pastoris and showed peroxidase activity with at least one of the substrates tested, thus enabling their development into commercial pure isoenzymes. (nih.gov)
  • Cysteamine and cysteine esters were found to be peroxidase-oxidase substrates for eosinophil peroxidase and myeloperoxidase, whereas other thiols tested were inactive or poorly active with these peroxidases. (biochemj.org)
  • These studies suggest that a main reason for thiols being poor peroxidase-oxidase substrates is because these thiols are poor peroxidatic substrates. (biochemj.org)
  • COX-2 is naturally inhibited by calcitriol (the active form of Vitamin D). Both the peroxidase and PTGS activities are inactivated during catalysis by mechanism-based, first-order processes, which means that PGHS-2 peroxidase or PTGS activities fall to zero within 1-2 minutes, even in the presence of sufficient substrates. (wikipedia.org)
  • PAP soluble complexes, as well as other immunoperoxidase reagents, are not recommended for tissues or cells in which endogenous peroxidase activity is difficult to suppress. (jacksonimmuno.com)
  • Yamamoto N, Suzuki S, Ota Z. Ultrastructural localization of endogenous peroxidase activity in Hashimoto's thyroiditis. (unboundmedicine.com)
  • TY - JOUR T1 - Ultrastructural localization of endogenous peroxidase activity in Hashimoto's thyroiditis. (unboundmedicine.com)
  • Glutathione peroxidase ( GPx ) ( EC 1.11.1.9 ) is the general name of an enzyme family with peroxidase activity whose main biological role is to protect the organism from oxidative damage. (wikipedia.org)
  • Pore opening is enhanced by peroxidase activity of cyt c gained upon its complexation with cardiolipin in the presence of reactive oxygen species. (aspetjournals.org)
  • Blocking access to the heme group has been proposed as an effective intervention method for reducing, if not eliminating, the peroxidase activity of cyt c . (aspetjournals.org)
  • In the present study, using a combination of druggability simulations, pharmacophore modeling, virtual screening, and in vitro fluorescence measurements to probe peroxidase activity, we identified three repurposable drugs and seven compounds that are validated to effectively inhibit the peroxidase activity of cyt c . (aspetjournals.org)
  • Amyloid beta, when bound to heme, has been shown to have peroxidase activity. (wikipedia.org)
  • Discussed functions of peroxidases range from cell wall synthesis, synthesis of prostaglandins, role in drug suppression of tuberculosis, and antibacterial activity. (rsc.org)
  • Uterine peroxidase enzyme activity has been studied as a marker for estrogen action in the uterus to help clarify the mechanism of estrogen action and its modulation by antiestrogens and progestins. (springer.com)
  • The current study suggests that peroxidase activity of DNAZymes is similar to HRP and it is able to degrade carbon-based nanomaterials. (rsc.org)
  • The team of University of Leicester researchers identified that glutathione peroxidase activity - a key antioxidant in cells - protects against symptoms of the disease in model organisms. (news-medical.net)
  • Their paper, Glutathione peroxidase activity is neuroprotective in models of Huntington's disease, was published in Nature Genetics on 25 August. (news-medical.net)
  • They found that glutathione peroxidase activity is robustly protective in these models of Huntington's disease. (news-medical.net)
  • The team now aim to further validate the observations regarding glutathione peroxidase activity, in order to understand whether this could have therapeutic relevance for Huntington's. (news-medical.net)
  • It appears that glutathione peroxidase activity is a robustly protective antioxidant approach which may have relevance for Huntington's disease. (news-medical.net)
  • Glutathione Peroxidase Assay Kit (Colorimetric) ab102530 can be used to quantitate the activity of all of the glutathione dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates. (abcam.com)
  • Glutathione Peroxidase (GPx, EC 1.11.1.9) is an enzyme family with peroxidase activity, and plays important role in protecting of organisms from oxidative damage. (abcam.com)
  • Glutathione Peroxidase Activity measured in biological fluids showing quantity (nmol) per 1 mL after 10 min of incubation. (abcam.com)
  • peroxidase " activity in an enzyme called cyclooxygenase. (wordnik.com)
  • The rational strategies for designing effective nanozymes with peroxidase-like activity will be a major step forward in this important and emerging field, because it requires the identification of predictive descriptors - structural characteristics of the nanomaterials that can be used as proxies for their peroxidase-like activities. (phys.org)
  • To meet this challenge, Wei and co-workers reported that the efficacy of a descriptor based on the occupancy of antibonding eg orbitals (i.e., eg occupancy) to predict and optimize the peroxidase-like activity of perovskite transition metal oxide (TMO) nanomaterials. (phys.org)
  • namely, perovskite TMOs with an occupancy of around one and zero (or two) exhibited the highest and lowest peroxidase-like activity, respectively. (phys.org)
  • The occupancy descriptor successfully predicted the peroxidase-like activity of binary TMOs with the same octahedral coordination geometries. (phys.org)
  • This study provides not only a straightforward and predictive activity descriptor for guiding the search for highly active peroxidase mimics but also molecular insights for understanding the mechanisms of the nanozyme catalyzed reactions. (phys.org)
  • eg occupancy as an effective descriptor for the catalytic activity of perovskite oxide-based peroxidase mimics, Nature Communications (2019). (phys.org)
  • Peroxidases are easily extracted from turnips and other root vegetables and provide a model enzyme for studying enzyme activity-how the rate of an enzyme-catalyzed reaction depends on biotic and abiotic factors. (flinnsci.com)
  • In Hashimoto's thyroiditis, TPO activity on the microvilli of follicular cells was more intense than that of normal thyroid tissue, but the intensity of the intracytoplasmic peroxidase reaction was generally weaker than that of Graves' or normal thyroid tissue. (unboundmedicine.com)
  • Heat stress- and heat shock transcription factor-dependent expression and activity of ascorbate peroxidase in Arabidopsis. (semanticscholar.org)
  • Preserve diluted (1-1,000ng/mL) HRP conjugates for more than six months at room temperature without significant loss of activity using Guardian Peroxidase Conjugate Stabilizer/Diluent. (fishersci.ca)
  • DESIGN: Using in-vitro cell models, experiments were performed that included glutathione peroxidase (GPX) activity, Amplex UltraRed, CM-H(2)DCFDA, Annexin V, 8-oxoguanine, phospho-H2A.X, quantitative real-time PCR and western blot assays. (biomedsearch.com)
  • The concentration of selenium and thiobarbituric acid reactive substances (TBARS) and the activity of glutathione peroxidase were determined in the blood of 34 tannery workers who worked in an area containing chromium compounds. (ilo.org)
  • The SKPO-1 protein contains the critical catalytic residues necessary for peroxidase activity, and in a whole animal assay, more H 2 O 2 was detected from the mutant compared to the wild type, consistent with the loss of an H 2 O 2 sink. (genetics.org)
  • Ionizing radiation induces cell death by initiating the selective peroxidation of cardiolipin (CL) in mitochondria by the peroxidase activity of its complex with cytochrome c leading to release of hemoprotein into the cytosol and commitment to the apoptotic program. (cdc.gov)
  • We found that TPP-IOA and TPP-ISA exerted strong specific liganding of heme-iron in cyt c/CL complex, effectively suppressed its peroxidase activity and CL peroxidation. (cdc.gov)
  • Antioxidant defense status was investigated in HIV-infected patients by measuring serum selenium, erythrocyte glutathione peroxidase (GSH-Px) activity, plasma thiol (-SH) and glutathione (GSH) concentrations along with the assessment of the clinical stage and surrogate markers of HIV-disease. (nature.com)
  • Unusual peroxidase activity of polynitroxylated pegylated hemoglobin: elimination of H(2)O(2) coupled with intramolecular oxidation of nitroxides. (cdc.gov)
  • While Hb(AcTPO)(12) has been shown to exert beneficial effects in a number of models of oxidative injury, its peroxidase activity has not been characterized thus far. (cdc.gov)
  • Here we report that Hb(AcTPOH)(12) exhibits peroxidase activity where H(2)O(2) is utilized for intramolecular oxidation of its TPOH residues to TPO. (cdc.gov)
  • With the Peroxidase Enzyme Activity Inquiry Lab Solution for AP ® Biology, students investigate the activity of turnip peroxidase by measuring its rate of reaction with hydrogen peroxide and a natural reducing agent. (flinnsci.com)
  • In estrogen receptor (ER)-positive breast cancer cell lines, very low expression of glutathione peroxidase-1 (GPX-1) activity and hgpx1 mRNA has been observed. (unboundmedicine.com)
  • VL - 55 IS - 4 N2 - In estrogen receptor (ER)-positive breast cancer cell lines, very low expression of glutathione peroxidase-1 (GPX-1) activity and hgpx1 mRNA has been observed. (unboundmedicine.com)
  • Protein families that serve as peroxidases include: Haem-using haem peroxidase and the related animal heme-dependent peroxidases DyP-type peroxidase family Catalase some haloperoxidase Di-haem cytochrome c peroxidase Non-heme Thiol: glutathione peroxidase, peroxiredoxin vanadium bromoperoxidase Alkyl hydroperoxide reductase Manganese peroxidase NADH peroxidase The glutathione peroxidase family consists of 8 known human isoforms. (wikipedia.org)
  • A majority of peroxidase protein sequences can be found in the PeroxiBase database. (wikipedia.org)
  • The EPX gene provides instructions for making the eosinophil peroxidase protein. (medlineplus.gov)
  • EPX gene mutations reduce or prevent eosinophil peroxidase production or result in a protein that is unstable and nonfunctional. (medlineplus.gov)
  • The invention relates to peroxidase colloidal gold biosensors that provide a detectable electrochemical response based on direct oxidation of a redox protein at an electrode surface. (google.com)
  • Peroxidase-Conjugated Protein A is a versatile reagent which is useful in many immunological assay systems. (mpbio.com)
  • A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-168 of E.coli Thiol Peroxidase The Recombinant E. coli Thiol Peroxidase Protein is derived from E. coli. (fishersci.com)
  • The Recombinant E. coli Thiol Peroxidase Protein has been validated for the following applications: SDS-Page. (fishersci.com)
  • Both the cyclooxygenase and the peroxidase active sites are located in the catalytic domain, which accounts for approximately 80% of the protein. (wikipedia.org)
  • Eosinophil peroxidase deficiency is a condition that affects certain white blood cells called eosinophils but causes no health problems in affected individuals. (medlineplus.gov)
  • One of these proteins is called eosinophil peroxidase. (medlineplus.gov)
  • In eosinophil peroxidase deficiency, eosinophils have little or no eosinophil peroxidase. (medlineplus.gov)
  • Because eosinophil peroxidase deficiency does not cause any health problems, this condition is often diagnosed when blood tests are done for other reasons or when a family member has been diagnosed with the condition. (medlineplus.gov)
  • Approximately 100 individuals with eosinophil peroxidase deficiency have been described in the scientific literature. (medlineplus.gov)
  • Eosinophil peroxidase deficiency is estimated to occur in 8.6 in 1,000 Yemenite Jews, in 3 in 1,000 North-African Jews, and in 1 in 1,000 Iraqi Jews. (medlineplus.gov)
  • and in Luxembourg, eosinophil peroxidase deficiency is thought to occur in 1 in 100,000 people. (medlineplus.gov)
  • Mutations in the EPX gene cause eosinophil peroxidase deficiency. (medlineplus.gov)
  • During an immune response, activated eosinophils release eosinophil peroxidase at the site of injury. (medlineplus.gov)
  • As a result, eosinophils have severely reduced amounts of eosinophil peroxidase or none at all. (medlineplus.gov)
  • Other proteins within affected eosinophils are normal, and while the cells lacking eosinophil peroxidase are smaller and may have structural changes, the loss of eosinophil peroxidase does not appear to impair the function of eosinophils. (medlineplus.gov)
  • Kutter D, Mueller-Hagedorn S, Forges T, Glaesener R. A case of eosinophil peroxidase deficiency. (medlineplus.gov)
  • Romano M, Patriarca P, Melo C, Baralle FE, Dri P. Hereditary eosinophil peroxidase deficiency: immunochemical and spectroscopic studies and evidence for a compound heterozygosity of the defect. (medlineplus.gov)
  • [2] Some evidence, though, indicates reduced levels of glutathione peroxidase 4 can increase life expectancy in mice. (wikipedia.org)
  • It has been shown that low levels of glutathione peroxidase as measured in the serum may be a contributing factor to vitiligo . (wikipedia.org)
  • Scatter dot plots representing the levels of glutathione peroxidase (GPx) in brain tissue in different mice groups. (abcam.com)
  • In addition, selenium is incorporated at the active site of many proteins, including glutathione peroxidase , which is particularly important for cancer protection. (wordnik.com)
  • Many of these peroxidases are functionally associated with members of the NADPH oxidase (NOX)/dual oxidase (DUOX) family of proteins. (genetics.org)
  • GPX1 is one of only a small number of proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. (prospecbio.com)
  • The catalytic domain is homologous to mammalian peroxidases such as myeloperoxidase. (wikipedia.org)
  • Animal heme-dependent peroxidases is a family of peroxidases. (wikipedia.org)
  • The peroxidase active site, which catalyzes the reduction of PGG2 to PGH2, is located on the other side of the molecule, at the heme binding site. (wikipedia.org)
  • Peroxidases can contain a heme cofactor in their active sites, or alternately redox-active cysteine or selenocysteine residues. (wikipedia.org)
  • Heme peroxidases are widely distributed in biological systems and are involved in a wide range of processes essential for life. (rsc.org)
  • This book provides a comprehensive single source of information on the various aspects of heme peroxidase structure, function and mechanism of action. (rsc.org)
  • Chapters written and edited by worldwide experts span a range of heme peroxidases from plants, yeast, bacteria and mammals. (rsc.org)
  • Heme Peroxidases serves as an essential text for those working in industry and academia in biochemistry and metallobiology. (rsc.org)
  • HEME-containing peroxidases play critical, wide-ranging roles in biological systems. (genetics.org)
  • A typical group of peroxidases are the haloperoxidases. (wikipedia.org)
  • The biochemical function of glutathione peroxidase is to reduce lipid hydroperoxides to their corresponding alcohols and to reduce free hydrogen peroxide to water. (wikipedia.org)
  • The presence of estrogen, however, stimulates the osteoclasts to increase production of the antioxidant glutathione peroxidase , which degrades the free-radical hydrogen peroxide into water and oxygen, rendering it harmless. (wordnik.com)
  • The latter is an endogenous enzyme that, like glutathione peroxidase , breaks down hydrogen peroxide into water and oxygen. (wordnik.com)
  • A peroxidase /phenolics/ascorbate system can scavenge hydrogen peroxide in plants. (wordnik.com)
  • Peroxidases that utilize ASCORBIC ACID as an electron donor to reduce HYDROGEN PEROXIDE to WATER. (semanticscholar.org)
  • Peroxidases protect plants and animals against cell damage by catalyzing the breakdown of hydrogen peroxide, a natural but toxic byproduct of aerobic respiration. (flinnsci.com)
  • Human cellular glutathione peroxidase 1 (hGPX1) is a selenium-dependent enzyme that participates in the detoxification of hydrogen peroxide and a wide range of organic peroxides. (aacrjournals.org)
  • Glutathione peroxidase 3 (GPX3) is a member of the glutathione peroxidase family, which acts in the detoxification of hydrogen peroxide. (prospecbio.com)
  • and a large C-terminal catalytic domain containing the cyclooxygenase and the peroxidase active sites. (wikipedia.org)
  • In this mini review, we discuss the peroxidase-like catalytic activities of C-dots and their applications in biosensing. (mdpi.com)
  • On the other hand, for an enzyme such as cytochrome c peroxidase, the compounds that donate electrons are very specific, due to a very narrow active site. (wikipedia.org)
  • Cytochrome c peroxidase is used as a soluble, easily purified model for cytochrome c oxidase. (wikipedia.org)
  • A mitochondria-targeted imidazole-substituted fatty acid inhibits cytochrome c peroxidase and mitigates radiation-induced death. (cdc.gov)
  • [2] So far, eight different isoforms of glutathione peroxidase (GPx1-8) have been identified in humans. (wikipedia.org)
  • Mice genetically engineered to lack glutathione peroxidase 1 (Gpx1 −/− mice) are grossly phenotypically normal and have normal lifespans, indicating this enzyme is not critical for life. (wikipedia.org)
  • We offer Glutathione Peroxidase 1/GPX1 Kits for use in common research applications: Functional. (novusbio.com)
  • Each Glutathione Peroxidase 1/GPX1 Kit is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
  • Choose from our Glutathione Peroxidase 1/GPX1 Kits. (novusbio.com)
  • Glutathione peroxidase 1 (GPX1) is a member of the glutathione peroxidase family, consisting of 8 identified glutathione peroxidases (Gpx1-8) in humans. (prospecbio.com)
  • Many members of the Solanaceae, notably Solanum melongena (eggplant/aubergine) and Capsicum chinense (the habanero/Scotch bonnet varieties of chili peppers) use Guaiacol and the enzyme guaiacol peroxidase as a defense against bacterial parasites such as Ralstonia solanacearum: the gene expression for this enzyme commences within minutes of bacterial attack. (wikipedia.org)
  • Does lack of glutathione peroxidase 1 gene expression exacerbate lung injury induced by neonatal hyperoxia in mice? (nih.gov)
  • Cytosolic ascorbate peroxidase 1 is a central component of the reactive oxygen gene network of Arabidopsis. (semanticscholar.org)
  • Soluble, ionically bound peroxidase (POD) and polyphenoloxidase (PPO) were extracted from the pulp of peach fruit during ripening at 20°C. Ionically bound form was purified 6.1-fold by DEAE-cellulose and Sephadex G-100 chromatography. (scielo.br)
  • In contrast, the soluble peroxidase form from tomato (Kokkinakis & Brooks, 1979) and papaya fruit (Silva et al , 1990) reach a maximum peak followed by a marked decrease at the initial levels. (scielo.br)
  • Ascorbate peroxidase 1 plays a key role in the response of Arabidopsis thaliana to stress combination. (semanticscholar.org)
  • Regulation and function of ascorbate peroxidase isoenzymes. (semanticscholar.org)
  • Photosynthetic electron transport regulates the expression of cytosolic ascorbate peroxidase genes in Arabidopsis during excess light stress. (semanticscholar.org)
  • From sequence analysis of three novel ascorbate peroxidases from Arabidopsis thaliana to structure, function and evolution of seven types of ascorbate peroxidase. (semanticscholar.org)
  • Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves. (semanticscholar.org)
  • Dr Robert Mason, Research Associate in the Department of Genetics, and first author of the study, said: 'In addition to glutathione peroxidase, this study has identified many genes that improve Huntington's 'symptoms' in yeast. (news-medical.net)
  • Sequence databases, ORF finding and ORF characterization were utilized to identify peroxidase genes from the 14871 isotigs generated by de novo assembly. (nih.gov)
  • Animals exposed to RNA interference (RNAi) of the putative peroxidase genes were screened for susceptibility to the human pathogen Enterococcus faecalis . (genetics.org)
  • Manganese peroxidase (MnP) was produced from white rot edible mushroom Pleurotus ostreatus on the culture filtrate. (scielo.br)
  • For detailed information about manganese peroxidase, go to the full flat file . (brenda-enzymes.org)
  • The sequences were manually reviewed and verified with Sanger sequencing of PCR amplified genomic fragments, resulting in the discovery of 28 secretory peroxidases, 23 of them previously unknown. (nih.gov)
  • Prakasha, A, Umesha, S. Biochemical and Molecular Variations of Guaiacol Peroxidase and Total Phenols in Bacterial Wilt Pathogenesis of Solanum melongena. (wikipedia.org)
  • Molecular model of a tryparedoxin peroxidase molecule from the parasitic protozoa Trypanosoma cruzi, the cause of Chagas disease. (sciencephoto.com)
  • It plays a role in the production of antioxidants containing selenium, such as glutathione peroxidase which is important in detoxification. (rainbow.coop)
  • In the glutathione peroxidase assay protocol, glutathione peroxidase (GPx) oxidizes GSH to produce GSSG as part of the reaction in which it reduces cumene hydroperoxide. (abcam.com)
  • Functional Studies - Glutathione Peroxidase Assay Kit (Colorimetric) (ab102530) George, Akash K et al. (abcam.com)
  • Functional Studies - Glutathione Peroxidase Assay Kit (Colorimetric) (ab102530) Liu, Baohai et al. (abcam.com)
  • A dedicated Glutathione Peroxidase control for use in the routine monitoring of the Randox Ransel assay on clinical chemistry analysers. (randox.com)
  • 2019) AAk gegen Schilddrüsen-spezifische Peroxidase. (springer.com)
  • The antioxidant enzyme glutathione peroxidase 4 (Gpx4) is a key regulator of oxidative stress-induced cell death. (nih.gov)
  • an oxidase layer atop the deposited colloidal gold adsorbed peroxidase wherein direct electron transfer to the conducting surface occurs in the presence of an analyte when the bioelectrode is suitably coupled with a reference/counter electrode. (google.com)
  • In the presence of peroxidases, TMB can act as an electron donor for the conversion of peroxides to water, changing the color of solution to a blue color equivalent to the degree of reactivity. (rockland-inc.com)
  • The abilities of various peroxidases to catalyse the peroxidase-oxidase oxidation of seven aminothiols were studied. (biochemj.org)
  • A. Mika, F. Buck, and S. Lüthje, "Membrane-bound class III peroxidases: identification, biochemical properties and sequence analysis of isoenzymes purified from maize ( Zea mays L.) roots," Journal of Proteomics , vol. 71, no. 4, pp. 412-424, 2008. (hindawi.com)
  • Identification and manual verification of the sequences of 28 HRP isoenzymes do not only contribute a set of peroxidases for industrial, biological and biomedical applications, but also provide valuable information on the reliability of the approach in identifying and characterizing a large group of isoenzymes. (nih.gov)
  • Since progesterone was found to inhibit peroxidase induction due to both estrone and diethylstilbestrol, as well as estradiol, it is considered unlikely that this antagonism relates to progestin-induced increases in uterine 17 β -hydroxysteroid dehydrogenase. (springer.com)
  • Isoflavones also inhibit thyroid peroxidase , which produces the thyroid hormones T3 and T4. (wordnik.com)
  • 1989. Polymerization of substituted anilines, phenols, and heterocyclic compounds by peroxidase in organic solvents. (wikipedia.org)
  • How do organic solvents affect peroxidase structure and function? (nih.gov)
  • Your search returned 15 Glutathione Peroxidase ELISA ELISA Kit across 1 supplier. (biocompare.com)
  • The INDO/ROHF/CI quantum chemical method has been used to calculate the electronic structure and spectra of two candidate peroxide intermediates of model peroxidases. (rti.org)
  • In order to identify the origin of these spectra and their differences, we have calculated the electronic structure and spectra of two possible forms of the peroxide intermediate of model peroxidases, one with a neutral peroxide and the other with an anionic form (OOH? (rti.org)
  • A process for the preparation of a phenolic resin which comprises reacting a phenol with a peroxidase or an oxidase enzyme and a peroxide in an organic solvent containing medium to generate phenolic radicals which react to form a phenolic resin. (freepatentsonline.com)
  • Thyroid peroxidase is an enzyme produced by the thyroid gland. (kidshealth.org)
  • Thyroid peroxidase (TPO) is an enzyme made in the thyroid gland that is important in the production of thyroid hormone . (rxlist.com)
  • Structural Characterization of Cytochrome c- under Peroxidase by Resonance Raman Scattering. (bell-labs.com)
  • Research at Iowa State University shows that the addition of soybean peroxidase to pig manure can reduce its environmental impacts in terms both of odours and harmful gases. (thepigsite.com)
  • Soybean peroxidase is an enzyme derived from soybean hulls. (umn.edu)
  • Commodity soybean cultivars contain various amounts of active peroxidase enzyme. (umn.edu)
  • This study evaluates alternative supply chain arrangements for moving soybean hulls containing peroxidase from producer to processor. (umn.edu)
  • Results suggest at current peroxidase levels in soybeans, supply chain arrangements involving soybean segregation offer cost advantages over the standard commodity supply chain. (umn.edu)
  • 1994). Appreciable levels of peroxidases involved in lignin biodegradation by Pleurotus species such as lignin peroxidase (LP) and MnP (Kirk and Farrell, 1987). (scielo.br)
  • While breakthroughs in peroxidase-like nanozymes have been made recently, most studies are based on trial-and-error strategies to identify and synthesize suitable peroxidase mimics. (phys.org)
  • TY - JOUR T1 - Expression of selenium-dependent glutathione peroxidase in human breast tumor cell lines. (unboundmedicine.com)