An enzyme isolated from horseradish which is able to act as an antigen. It is frequently used as a histochemical tracer for light and electron microscopy. Its antigenicity has permitted its use as a combined antigen and marker in experimental immunology.
An enzyme catalyzing the oxidation of 2 moles of glutathione in the presence of hydrogen peroxide to yield oxidized glutathione and water. EC
A hemeprotein from leukocytes. Deficiency of this enzyme leads to a hereditary disorder coupled with disseminated moniliasis. It catalyzes the conversion of a donor and peroxide to an oxidized donor and water. EC
A hemeprotein which catalyzes the oxidation of ferrocytochrome c to ferricytochrome c in the presence of hydrogen peroxide. EC
Peroxidases that utilize ASCORBIC ACID as an electron donor to reduce HYDROGEN PEROXIDE to WATER. The reaction results in the production of monodehydroascorbic acid and DEHYDROASCORBIC ACID.
A 66-kDa peroxidase found in EOSINOPHIL granules. Eosinophil peroxidase is a cationic protein with a pI of 10.8 and is comprised of a heavy chain subunit and a light chain subunit. It possesses cytotoxic activity towards BACTERIA and other organisms, which is attributed to its peroxidase activity.
A hemeprotein that catalyzes the oxidation of the iodide radical to iodine with the subsequent iodination of many organic compounds, particularly proteins. EC
A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials.
An agent thought to have disinfectant properties and used as an expectorant. (From Martindale, The Extra Pharmacopoeia, 30th ed, p747)
An oxidoreductase that catalyzes the conversion of HYDROGEN PEROXIDE to water and oxygen. It is present in many animal cells. A deficiency of this enzyme results in ACATALASIA.
An element with the atomic symbol Se, atomic number 34, and atomic weight 78.96. It is an essential micronutrient for mammals and other animals but is toxic in large amounts. Selenium protects intracellular structures against oxidative damage. It is an essential component of GLUTATHIONE PEROXIDASE.
An enzyme derived from cow's milk. It catalyzes the radioiodination of tyrosine and its derivatives and of peptides containing tyrosine.
A family of ubiquitously-expressed peroxidases that play a role in the reduction of a broad spectrum of PEROXIDES like HYDROGEN PEROXIDE; LIPID PEROXIDES and peroxinitrite. They are found in a wide range of organisms, such as BACTERIA; PLANTS; and MAMMALS. The enzyme requires the presence of a thiol-containing intermediate such as THIOREDOXIN as a reducing cofactor.
The most abundant natural aromatic organic polymer found in all vascular plants. Lignin together with cellulose and hemicellulose are the major cell wall components of the fibers of all wood and grass species. Lignin is composed of coniferyl, p-coumaryl, and sinapyl alcohols in varying ratios in different plant species. (From Merck Index, 11th ed)
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
An oxidoreductase that catalyzes the reaction between superoxide anions and hydrogen to yield molecular oxygen and hydrogen peroxide. The enzyme protects the cell against dangerous levels of superoxide. EC
An enzyme that catalyzes the chlorination of a range of organic molecules, forming stable carbon-chloride bonds. EC
A phylum of fungi that produce their sexual spores (basidiospores) on the outside of the basidium. It includes forms commonly known as mushrooms, boletes, puffballs, earthstars, stinkhorns, bird's-nest fungi, jelly fungi, bracket or shelf fungi, and rust and smut fungi.
Alcohols derived from the aryl radical (C6H5CH2-) and defined by C6H5CHOH. The concept includes derivatives with any substituents on the benzene ring.
Naturally occurring or synthetic substances that inhibit or retard the oxidation of a substance to which it is added. They counteract the harmful and damaging effects of oxidation in animal tissues.
Catalyzes the oxidation of GLUTATHIONE to GLUTATHIONE DISULFIDE in the presence of NADP+. Deficiency in the enzyme is associated with HEMOLYTIC ANEMIA. Formerly listed as EC
A disturbance in the prooxidant-antioxidant balance in favor of the former, leading to potential damage. Indicators of oxidative stress include damaged DNA bases, protein oxidation products, and lipid peroxidation products (Sies, Oxidative Stress, 1991, pxv-xvi).
The color-furnishing portion of hemoglobin. It is found free in tissues and as the prosthetic group in many hemeproteins.
A group of compounds that contain a bivalent O-O group, i.e., the oxygen atoms are univalent. They can either be inorganic or organic in nature. Such compounds release atomic (nascent) oxygen readily. Thus they are strong oxidizing agents and fire hazards when in contact with combustible materials, especially under high-temperature conditions. The chief industrial uses of peroxides are as oxidizing agents, bleaching agents, and initiators of polymerization. (From Hawley's Condensed Chemical Dictionary, 11th ed)
A tripeptide with many roles in cells. It conjugates to drugs to make them more soluble for excretion, is a cofactor for some enzymes, is involved in protein disulfide bond rearrangement and reduces peroxides.
Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.
Peroxidase catalyzed oxidation of lipids using hydrogen peroxide as an electron acceptor.
The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
The rate dynamics in chemical or physical systems.
A genus of fungi in the family Corticiaceae, order Stereales, that degrades lignin. The white-rot fungus Phanerochaete chrysosporium is a frequently used species in research.
Very toxic industrial chemicals. They are absorbed through the skin, causing lethal blood, bladder, liver, and kidney damage and are potent, broad-spectrum carcinogens in most species.
A genus of black-spored basidiomycetous fungi of the family Coprinaceae, order Agaricales; some species are edible.
Inorganic binary compounds of iodine or the I- ion.
Selenoproteins are proteins that specifically incorporate SELENOCYSTEINE into their amino acid chain. Most selenoproteins are enzymes with the selenocysteine residues being responsible for their catalytic functions.
Peroxides produced in the presence of a free radical by the oxidation of unsaturated fatty acids in the cell in the presence of molecular oxygen. The formation of lipid peroxides results in the destruction of the original lipid leading to the loss of integrity of the membranes. They therefore cause a variety of toxic effects in vivo and their formation is considered a pathological process in biological systems. Their formation can be inhibited by antioxidants, such as vitamin E, structural separation or low oxygen tension.
The lectin wheatgerm agglutinin conjugated to the enzyme HORSERADISH PEROXIDASE. It is widely used for tracing neural pathways.
A genus of basidiomycetous fungi, family POLYPORACEAE, order POLYPORALES, that grows on logs or tree stumps in shelflike layers. The species P. ostreatus, the oyster mushroom, is a choice edible species and is the most frequently encountered member of the genus in eastern North America. (Alexopoulos et al., Introductory Mycology, 4th ed, p531)
A selenium compound with the molecular formula H2SO3. It used as a source of SELENIUM, especially for patients that develop selenium deficiency following prolonged PARENTERAL NUTRITION.
Highly reactive molecules with an unsatisfied electron valence pair. Free radicals are produced in both normal and pathological processes. They are proven or suspected agents of tissue damage in a wide variety of circumstances including radiation, damage from environment chemicals, and aging. Natural and pharmacological prevention of free radical damage is being actively investigated.
The dialdehyde of malonic acid.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A peroxiredoxin that is a cytosolic bifunctional enzyme. It functions as a peroxiredoxin via a single redox-active cysteine and also contains a Ca2+-independent acidic phospholipase A2 activity.
The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.
A highly vascularized endocrine gland consisting of two lobes joined by a thin band of tissue with one lobe on each side of the TRACHEA. It secretes THYROID HORMONES from the follicular cells and CALCITONIN from the parafollicular cells thereby regulating METABOLISM and CALCIUM level in blood, respectively.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.
A THIOREDOXIN-dependent hydroperoxidase that is localized in the mitochondrial matrix. The enzyme plays a crucial role in protecting mitochondrial components from elevated levels of HYDROGEN PEROXIDE.
An extracellular selenoprotein that contains most of the SELENIUM in PLASMA. Selenoprotein P functions as an antioxidant and appears to transport selenium from the LIVER to peripheral tissues.
Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.
Proteins that contain an iron-porphyrin, or heme, prosthetic group resembling that of hemoglobin. (From Lehninger, Principles of Biochemistry, 1982, p480)
A naturally occurring amino acid in both eukaryotic and prokaryotic organisms. It is found in tRNAs and in the catalytic site of some enzymes. The genes for glutathione peroxidase and formate dehydrogenase contain the TGA codon, which codes for this amino acid.
A family of bracket fungi, order POLYPORALES, living in decaying plant matter and timber.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
A six carbon compound related to glucose. It is found naturally in citrus fruits and many vegetables. Ascorbic acid is an essential nutrient in human diets, and necessary to maintain connective tissue and bone. Its biologically active form, vitamin C, functions as a reducing agent and coenzyme in several metabolic pathways. Vitamin C is considered an antioxidant.
An order of fungi in the phylum BASIDIOMYCOTA having macroscopic basidiocarps. The members are characterized by their saprophytic activities as decomposers, particularly in the degradation of CELLULOSE and LIGNIN. A large number of species in the order have been used medicinally. (From Alexopoulos, Introductory Mycology, 4th ed, pp504-68)
Molecules or ions formed by the incomplete one-electron reduction of oxygen. These reactive oxygen intermediates include SINGLET OXYGEN; SUPEROXIDES; PEROXIDES; HYDROXYL RADICAL; and HYPOCHLOROUS ACID. They contribute to the microbicidal activity of PHAGOCYTES, regulation of signal transduction and gene expression, and the oxidative damage to NUCLEIC ACIDS; PROTEINS; and LIPIDS.
Low-molecular-weight end products, probably malondialdehyde, that are formed during the decomposition of lipid peroxidation products. These compounds react with thiobarbituric acid to form a fluorescent red adduct.
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
An enzyme of the oxidoreductase class that catalyzes the conversion of beta-D-glucose and oxygen to D-glucono-1,5-lactone and peroxide. It is a flavoprotein, highly specific for beta-D-glucose. The enzyme is produced by Penicillium notatum and other fungi and has antibacterial activity in the presence of glucose and oxygen. It is used to estimate glucose concentration in blood or urine samples through the formation of colored dyes by the hydrogen peroxide produced in the reaction. (From Enzyme Nomenclature, 1992) EC
Inorganic salts of HYDROGEN CYANIDE containing the -CN radical. The concept also includes isocyanides. It is distinguished from NITRILES, which denotes organic compounds containing the -CN radical.
Granular leukocytes with a nucleus that usually has two lobes connected by a slender thread of chromatin, and cytoplasm containing coarse, round granules that are uniform in size and stainable by eosin.
Enzymes which are immobilized on or in a variety of water-soluble or water-insoluble matrices with little or no loss of their catalytic activity. Since they can be reused continuously, immobilized enzymes have found wide application in the industrial, medical and research fields.
A trace element with atomic symbol Mn, atomic number 25, and atomic weight 54.94. It is concentrated in cell mitochondria, mostly in the pituitary gland, liver, pancreas, kidney, and bone, influences the synthesis of mucopolysaccharides, stimulates hepatic synthesis of cholesterol and fatty acids, and is a cofactor in many enzymes, including arginase and alkaline phosphatase in the liver. (From AMA Drug Evaluations Annual 1992, p2035)
A transferase that catalyzes the addition of aliphatic, aromatic, or heterocyclic FREE RADICALS as well as EPOXIDES and arene oxides to GLUTATHIONE. Addition takes place at the SULFUR. It also catalyzes the reduction of polyol nitrate by glutathione to polyol and nitrite.
An extensive order of basidiomycetous fungi whose fruiting bodies are commonly called mushrooms.
A direct-acting oxidative stress-inducing agent used to examine the effects of oxidant stress on Ca(2+)-dependent signal transduction in vascular endothelial cells. It is also used as a catalyst in polymerization reactions and to introduce peroxy groups into organic molecules.
A plant genus of the family BRASSICACEAE known for the root used in hot SPICES. It is also the source of HORSERADISH PEROXIDASE which is widely used in laboratories.
Organic derivatives of thiocyanic acid which contain the general formula R-SCN.
Diagnostic aid in pancreas function determination.
An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.
Inorganic salts of the hypothetical acid ferrocyanic acid (H4Fe(CN)6).
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Electron-accepting molecules in chemical reactions in which electrons are transferred from one molecule to another (OXIDATION-REDUCTION).
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Benzene derivatives that include one or more hydroxyl groups attached to the ring structure.
A copper-containing oxidoreductase enzyme that catalyzes the oxidation of 4-benzenediol to 4-benzosemiquinone. It also has activity towards a variety of O-quinols and P-quinols. It primarily found in FUNGI and is involved in LIGNIN degradation, pigment biosynthesis and detoxification of lignin-derived products.
Analysis of the intensity of Raman scattering of monochromatic light as a function of frequency of the scattered light.
The engulfing of liquids by cells by a process of invagination and closure of the cell membrane to form fluid-filled vacuoles.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
A GLUTATHIONE dimer formed by a disulfide bond between the cysteine sulfhydryl side chains during the course of being oxidized.
A group of compounds that are derivatives of methoxybenzene and contain the general formula R-C7H7O.
Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The disodium salt of selenious acid. It is used therapeutically to supply the trace element selenium and is prepared by the reaction of SELENIUM DIOXIDE with SODIUM HYDROXIDE.
A non-selective post-emergence, translocated herbicide. According to the Seventh Annual Report on Carcinogens (PB95-109781, 1994) this substance may reasonably be anticipated to be a carcinogen. (From Merck Index, 12th ed) It is an irreversible inhibitor of CATALASE, and thus impairs activity of peroxisomes.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
Inflammatory disease of the THYROID GLAND due to autoimmune responses leading to lymphocytic infiltration of the gland. It is characterized by the presence of circulating thyroid antigen-specific T-CELLS and thyroid AUTOANTIBODIES. The clinical signs can range from HYPOTHYROIDISM to THYROTOXICOSIS depending on the type of autoimmune thyroiditis.
Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.
Salts of hydrobromic acid, HBr, with the bromine atom in the 1- oxidation state. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A generic descriptor for all TOCOPHEROLS and TOCOTRIENOLS that exhibit ALPHA-TOCOPHEROL activity. By virtue of the phenolic hydrogen on the 2H-1-benzopyran-6-ol nucleus, these compounds exhibit varying degree of antioxidant activity, depending on the site and number of methyl groups and the type of ISOPRENOIDS.
Hydrogen-donating proteins that participates in a variety of biochemical reactions including ribonucleotide reduction and reduction of PEROXIREDOXINS. Thioredoxin is oxidized from a dithiol to a disulfide when acting as a reducing cofactor. The disulfide form is then reduced by NADPH in a reaction catalyzed by THIOREDOXIN REDUCTASE.
A genus of fungi in the family Coriolaceae.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
Organic compounds which contain selenium as an integral part of the molecule.
Proteins prepared by recombinant DNA technology.
A FLAVOPROTEIN enzyme that catalyzes the oxidation of THIOREDOXINS to thioredoxin disulfide in the presence of NADP+. It was formerly listed as EC
A group of physiologically active prostaglandin endoperoxides. They are precursors in the biosynthesis of prostaglandins and thromboxanes. Most frequently encountered member of this group is the prostaglandin G2.
The measurement of the amplitude of the components of a complex waveform throughout the frequency range of the waveform. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Compounds containing the -SH radical.
Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.
Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)
Diazo derivatives of aniline, used as a reagent for sugars, ketones, and aldehydes. (Dorland, 28th ed)
A plant genus in the family LILIACEAE (sometimes placed in Asparagaceae) that contains ECDYSTEROIDS and is an ingredient of Siotone. The shoots are used as a vegetable and the roots are used in FOLK MEDICINE.
Elements of limited time intervals, contributing to particular results or situations.
Hydroxycinnamic acid and its derivatives. Act as activators of the indoleacetic acid oxidizing system, thereby producing a decrease in the endogenous level of bound indoleacetic acid in plants.
The facilitation of biochemical reactions with the aid of naturally occurring catalysts such as ENZYMES.
An antiseptic and disinfectant aromatic alcohol.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.
A cytochrome oxidase inhibitor which is a nitridizing agent and an inhibitor of terminal oxidation. (From Merck Index, 12th ed)
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
A product from the iodination of MONOIODOTYROSINE. In the biosynthesis of thyroid hormones, diiodotyrosine residues are coupled with other monoiodotyrosine or diiodotyrosine residues to form T4 or T3 thyroid hormones (THYROXINE and TRIIODOTHYRONINE).
The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.

Stimulation of renin release from rabbit renal cortex by arachidonic acid and prostaglandin endoperoxides. (1/2940)

The mechanism by which renal prostaglandins stimulate renin secretion in vivo is unknown. In this in vitro study we measured the effects of activation of the prostaglandin (PG) system on renin release from slices of rabbit renal cortex. The PG precursor arachidonic acid (C20:4), a natural PG endoperoxide (PGG2), two stable synthetic PG endoperoxide analogues (EPA I and II), PGE2, PGF2alpha, and two different PG synthesis inhibitors [indomethacin and 5,8,11,14-eicosatetraynoic acid (ETA)] were used to evaluate the possibility of a direct action of the cortical PG system on renin secretion. Renin release increased significantly with time after addition of C20:4, PGG2, EPA I, and EPA II to the incubation medium. Stimulation of renin release was se-related for C20:4 in concentrations of 0.6 to 4.5 X 10(-6) M, for EPA I in concentrations of 0.7 to 2.8 X 10(-6) M, and for EPA II in concentrations of 1.4 to 14.0 X 10(-6) M. Indomethacin (10(-4) M) and ETA (10(-4) M) significantly decreased basal renin release as well as the renin release stimulated by C20:4 and EPA I. PGE2(10(-12) to 10(-6) M) had no effect on renin release, whereas PGF2alpha (10(-12) to 10(-6) M) decreased renin release in a dose-dependent manner. These data raise the possibility of a direct action of the renal cortical PG system on renin secretion. The results further indicate that stimulation of renin release by C20:4 may depend more specifically on the action of PG endoperoxides than on the primary prostaglandins.  (+info)

Isolation and purification of rat mammary tumor peroxidase. (2/2940)

7,12-Dimethylbenz(a)anthracene-induced rat mammary tumors often contain high levels of the enzyme perioxidase, a putative marker of estrogen dependence. This enzyme can be effectively extracted with 0.5 M CaCl2, giving rise to a soluble peroxidase with a molecular weight of about 50,000 as determined by gel filtration. This is the same size as the estrogen-induced peroxidase of rat uterus but smaller than other mammalian peroxidases. Further purification of the rat mammary tumor peroxidase by concanavalin A-Sepharose chromatography and hydrophobic interaction chromatography on phenyl Sepharose provides a 640-fold purification of the enzyme.  (+info)

Cloning of the peroxiredoxin gene family in rats and characterization of the fourth member. (3/2940)

Peroxiredoxin (PRx) exhibits thioredoxin-dependent peroxidase activity and constitutes a family of proteins. Four members of genes from rat tissues were isolated by PCR using degenerated primers based on the sequences which encode a pair of highly conserved Cys-containing domains, and were then cloned to full-length cDNAs. These included two genes which have previously been isolated in rats, PRx I and PRx II, and two rat homologues of PRx III and PRx IV. We showed, for the first time, the simultaneous expression of all four genes in various rat tissues by Northern blotting. Since a discrepancy exists regarding cellular distribution, we further characterized PRx IV by expressing it in COS-1 cells. This clearly demonstrates that PRx IV is a secretory form and functions within the extracellular space.  (+info)

Purification and characterization of a novel peroxidase from Geotrichum candidum dec 1 involved in decolorization of dyes. (4/2940)

A peroxidase (DyP) involved in the decolorization of dyes and produced by the fungus strain Geotrichum candidum Dec 1 was purified. DyP, a glycoprotein, is glycosylated with N-acetylglucosamine and mannose (17%) and has a molecular mass of 60 kDa and an isoelectric point (pI) of 3.8. The absorption spectrum of DyP exhibited a Soret band at 406 nm corresponding to a hemoprotein, and its Na2S2O4-reduced form revealed a peak at 556 nm that indicates the presence of a protoheme as its prosthetic group. Nine of the 21 types of dyes that were decolorized by Dec 1 cells were decolorized by DyP; in particular, anthraquinone dyes were highly decolorized. DyP also oxidized 2,6-dimethoxyphenol and guaiacol but not veratryl alcohol. The optimal temperature for DyP activity was 30 degrees C, and DyP activity was stable even after incubation at 50 degrees C for 11 h.  (+info)

Phospholipid hydroperoxide cysteine peroxidase activity of human serum albumin. (5/2940)

Human serum albumin (HSA) reduced the phospholipid hydroperoxide, 1-palmitoyl-2-(13-hydroperoxy-cis-9, trans-11-octadecadienoyl)-l-3-phosphatidylcholine (PLPC-OOH) to the corresponding hydroxy-derivative with a high apparent affinity (Km=9. 23+/-0.95 microM). Removal of bound lipid during purification increased this activity. At physiological concentration, HSA reduced the phospholipid hydroperoxide in the absence of a cofactor. However, in the presence of a cofactor (reductant), the rate of the reaction was increased. All of the major aminothiols in plasma could act as reductants, the best being the most abundant, cysteine (Km=600+/-80 microM). For every nanomole of PLPC-OOH reduced by HSA, 1.26 nmol of cystine was formed, indicating a reaction stoichiometry of 1 mol PLPC-OOH to 2 mol cysteine. We used chemical modification to determine which amino acid residues on HSA were responsible for the activity. Oxidation of thiol group(s) by N-ethylmaleimide led to a reduction in the rate of activity, whereas reduction of thiols by either dithiothreitol or the angiotensin-converting enzyme inhibitor, captopril, increased the activity. Both N-ethylmaleimide-modified HSA and dithiothreitol-treated HSA exhibited increased apparent affinities for PLPC-OOH. For a range of preparations of albumin with different modifications, the activity on PLPC-OOH was dependent on the amount of free thiol groups on the albumin (correlation coefficient=0.91). Patients with lowered albumin concentrations after septic shock showed lowered total plasma thiol concentrations and decreased phospholipid hydroperoxide cysteine peroxidase (PHCPx) activities. These results therefore show for the first time that HSA exhibits PHCPx activity, and that the majority of the activity depends on the presence of reduced thiol group(s) on the albumin.  (+info)

Use of site-directed mutagenesis to probe the structure, function and isoniazid activation of the catalase/peroxidase, KatG, from Mycobacterium tuberculosis. (6/2940)

A series of mutants bearing single amino acid substitutions often encountered in the catalase/peroxidase, KatG, from isoniazid-resistant isolates of Mycobacterium tuberculosis has been produced by site-directed mutagenesis. The resultant enzymes were overexpressed, purified and characterized. Replacing Cys-20 by Ser abolished disulphide-bridge formation, but did not affect either dimerization of the enzyme or catalysis. The substitution of Thr-275, which is probably involved in electron transfer from the haem, by proline resulted in a highly unstable enzyme with insignificant enzyme activities. The most commonly occurring substitution in drug-resistant clinical isolates is the replacement of Ser-315 by Thr; this lowered catalase and peroxidase activities by 50% and caused a significant decrease in the KatG-mediated inhibition of the activity of the NADH-dependent enoyl-[acyl-carrier protein] reductase, InhA, in vitro. The ability of this enzyme to produce free radicals from isoniazid was severely impaired, as judged by its loss of NitroBlue Tetrazolium reduction activity. Replacement of Leu-587 by Pro resulted in marked instability of KatG, indicating that the C-terminal domain is also important for structural and functional integrity.  (+info)

Direct interaction of lignin and lignin peroxidase from Phanerochaete chrysosporium. (7/2940)

Binding properties of lignin peroxidase (LiP) from the basidiomycete Phanerochaete chrysosporium against a synthetic lignin (dehydrogenated polymerizate, DHP) were studied with a resonant mirror biosensor. Among several ligninolytic enzymes, only LiP specifically binds to DHP. Kinetic analysis revealed that the binding was reversible, and that the dissociation equilibrium constant was 330 microM. The LiP-DHP interaction was controlled by the ionization group with a pKa of 5.3, strongly suggesting that a specific amino acid residue plays a role in lignin binding. A one-electron transfer from DHP to oxidized intermediates LiP compounds I and II (LiPI and LiPII) was characterized by using a stopped-flow technique, showing that binding interactions of DHP with LiPI and LiPII led to saturation kinetics. The dissociation equilibrium constants for LiPI-DHP and LiPII-DHP interactions were calculated to be 350 and 250 microM, and the first-order rate constants for electron transfer from DHP to LiPI and to LiPII were calculated to be 46 and 16 s-1, respectively. These kinetic and spectral studies strongly suggest that LiP is capable of oxidizing lignin directly at the protein surface by a long-range electron transfer process. A close look at the crystal structure suggested that LiP possesses His-239 as a possible lignin-binding site on the surface, which is linked to Asp-238. This Asp residue is hydrogen-bonded to the proximal His-176. This His-Asp...proximal-His motif would be a possible electron transfer route to oxidize polymeric lignin.  (+info)

Eosinophil peroxidase increases membrane permeability in mammalian urinary bladder epithelium. (8/2940)

Eosinophil peroxidase (EPO), a cationic protein found in eosinophils, has been reported to be cytotoxic independent of its peroxidase activity. This study investigated with electrophysiological methods whether EPO is toxic to mammalian urinary bladder epithelium. Results indicate that EPO, when added to the mucosal solution, increases apical membrane conductance of urinary bladder epithelium only when the apical membrane potential is cell interior negative. The EPO-induced conductance was concentration dependent, with a maximum conductance of 411 microseconds/cm2 and a Michaelis-Menten constant of 113 nM. The EPO-induced conductance was nonselective for K+ and Cl-. The conductance was partially reversed using voltage but not by removal of EPO from the bulk solution. Mucosal Ca2+ reversed the EPO-induced conductance by a mechanism involving reversible block of the conductance. Prolonged exposure (up to 1 h) to EPO was toxic to the urinary bladder epithelium, as indicated by an irreversible increase in transepithelial conductance. These results suggest that EPO is indeed toxic to urinary bladder epithelium via a mechanism that involves an increase in membrane permeability.  (+info)

In order to understand the mechanism of molecular interactions at the active site of Tryparedoxin Peroxidase (Try P), homology modeling and docking studies were performed. We generated a Three-Dimensional (3D) model of target protein based on the Crystal structure of Leishmania Major Try PI (PDB ID: 3TUE) using modeler software. Docking analysis was carried out to study the effects of methotrexate on Tryparedoxin Peroxidase (Try P). Inhibition of the Tryparedoxin peroxidase interaction has become a new therapeutic strategy in treating leishmaniasis. Docking analysis was carried out to study the effects of methotrexate on Tryparedoxin Peroxidase (TryP). Tryparedoxin peroxidase of Trypanosomatidae family functions as antioxidant through their peroxidase and peroxynitrite reductase activities. The theoretical docking study, conducted on a sample previously reported for anti-cancer properties of Methotrexate at the binding site of 3D models of Tryparedoxin Peroxidase of Leishmania braziliensis (L. ...
TY - JOUR. T1 - Expression of a moderately anionic peroxidase is induced by aluminum treatment in tobacco cells. T2 - Possible involvement of peroxidase isozymes in aluminum ion stress. AU - Ezaki, Bunichi. AU - Tsugita, Shinobu. AU - Matsumoto, Hideaki. PY - 1996/1. Y1 - 1996/1. N2 - To clarify the mechanism of aluminum (Al) toxicity and Al tolerance, we isolated a new clone (pAL201) from a tobacco cDNA library. Northern blot hybridization analysis indicated that the expression of pAL201 is induced by Al treatment and phosphate (Pi) starvation. The complete cDNA sequence suggested that this clone encodes a moderately anionic peroxidase (EC Analysis by isoelectric focussing indicated that a moderately anionic peroxidase (approximately pI 6.7) and two cationic peroxidases (pI 9.2 and 9.7) in the soluble fraction are activated by Al treatment and Pi starvation, while two moderately anionic isozymes are repressed by these stresses. We suppose that Al ion stress can control the activity ...
Fungal secretory peroxidases mediate fundamental ecological functions in the conversion and degradation of plant biomass. Many of these enzymes have strong oxidizing activities towards aromatic compounds and are involved in the degradation of plant cell wall (lignin) and humus. They comprise three major groups: class II peroxidases (including lignin peroxidase, manganese peroxidase, versatile peroxidase and generic peroxidase), dye-decolorizing peroxidases, and heme-thiolate peroxidases (e.g. unspecific/aromatic peroxygenase, chloroperoxidase). Here, we have repeatedly observed a widespread expression of all major peroxidase groups in leaf and needle litter across a range of forest ecosystems (e.g. Fagus, Picea, Acer, Quercus, and Populus spp.), which are widespread in Europe and North America. Manganese peroxidases and unspecific peroxygenases were found expressed in all nine investigated forest sites, and dye-decolorizing peroxidases were observed in five of the nine sites, thereby indicating
Hydrogen peroxide is thought to regulate cellular processes by direct oxidation of numerous cellular proteins, whereas antioxidants, most notably thiol peroxidases, are thought to reduce peroxides and inhibit H2O2 response. However, thiol peroxidases have also been implicated in activation of transcription factors and signaling. It remains unclear if these enzymes stimulate or inhibit redox regulation and whether this regulation is widespread or limited to a few cellular components. Herein, we found that Saccharomyces cerevisiae cells lacking all eight thiol peroxidases were viable and withstood redox stresses. They transcriptionally responded to various redox treatments, but were unable to activate and repress gene expression in response to H2O2. Further studies involving redox transcription factors suggested that thiol peroxidases are major regulators of global gene expression in response to H2O2. The data suggest that thiol peroxidases sense and transfer oxidative signals to the signaling ...
Heme peroxidases are widely distributed in biological systems and are involved in a wide range of processes essential for life. This book provides a comprehensive single source of information on the various aspects of heme peroxidase structure, function and mechanism of action. Chapters written and edited by worldwide experts span a range of heme peroxidases from plants, yeast, bacteria and mammals. Discussed functions of peroxidases range from cell wall synthesis, synthesis of prostaglandins, role in drug suppression of tuberculosis, and antibacterial activity. Included is a discussion of peroxidases that also act as catalases and oxygenases. Heme Peroxidases serves as an essential text for those working in industry and academia in biochemistry and metallobiology.
Peroxidase Substrates for Western Blotting, Immunohistochemistry, and ELISAPeroxidase Enzymes and Reagents Technical Information
Minute Peroxidase Suppressor is designed to inhibit endogenous peroxidase activity commonly encountered in immunohistochemistry (IHC) procedures. Inhibiting endogenous peroxidase activity is essential for avoiding false positive and reduce the background of IHC. Many chemical agents such as H2O2 and sodium aside have been used for this purpose but the inhibition is usually incomplete. Minute TM HRP Suppressor is a mixture of several potent HRP inhibitors and the result is a complete inhibitionof endogenous peroxidase activity. The suppressor can be used in regular IHC as well as HRP-mediated super sensitive procedures such as tyramide signal amplification (TSA). Major Features: Very stable at RT, ready to use and irreversible inhibition of endogenous peroxidase activity. Significantly reduce the background of sensitive assays using HRP. 1. After cell or tissue samples are prepared on slide, perform antigen retrieval step if necessary. 2. Block non-specific sites in the samples with normal serum ...
Infinite Enzymes Manganese Peroxidase Enzyme (MnP) is a recombinant peroxidase from Phanerochaete chrysosporium produced in corn seed. Request Quote now!
Alterations in Soluble Class III Peroxidases of Maize Shoots by Flooding Stress. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Thiol peroxidases are critical enzymes in the redox control of cellular processes that function by reducing low levels of hydroperoxides and regulating redox signaling. These proteins were also shown to regulate genome stability, but how their dysfunction affects the actual mutations in the genome is not known. Saccharomyces cerevisiae has eight thiol peroxidases of glutathione peroxidase and peroxiredoxin families, and the mutant lacking all these genes (Δ8) is viable. In this study, we employed two independent Δ8 isolates to analyze the genome-wide mutation spectrum that results from deficiency in these enzymes. Deletion of these genes was accompanied by a dramatic increase in point mutations, many of which clustered in close proximity and scattered throughout the genome, suggesting strong mutational bias. We further subjected multiple lines of wild-type and Δ8 cells to long-term mutation accumulation, followed by genome sequencing and phenotypic characterization. Δ8 lines showed a ...
White-rot fungi (WRF) are capable of degrading complex organic compounds such as lignin, and the enzymes that enable these processes can be used for the detoxification of recalcitrant organopollutants. The aim of this study is to evaluate a system based on the use of an in vitro ligninolytic enzyme for the detoxification of recalcitrant dye pollutants. The dyes selected for investigation were the anionic and cationic commercial azo dyes, basic blue 41 (BB41), acid black 1 (AB1), and reactive black 5 (RB5). A supernatant, cell-free culture of WRF with manganese peroxidase activity was used to investigate its degradative capacity under various conditions, and concentrations of cofactors, H2O2 and Mn2+. The assays were carried out using a 22 experimental designs whose variables were concentration of Mn2+ (33 and 1,000 μM) and semicontinuous dosage of the H2O2 (0.02 and 0.10 μmol) added at a frequency of 0.2 min−1. The response variables analyzed were the efficiency and the initial rate of the ...
The main objective of this study was to investigate the effect of adding a pretreatment stage in conventional hydrogen peroxide bleaching of high yield pulps. To achieve this, three main variables considered crucial in the effectiveness of manganese peroxidase on the oxidation of lignin substructures, were chosen to evaluate the effect of this enzyme on high yield pulps. The responses to evaluate this effect were brightness, brightness reversion, pulp viscosity, and yield. Other properties included opacity, scattering and absorption coefficients, and the Hunter Lab values. The pulps were bleached using two sequences Q(MnP), and Q(MnP)QP, to assess the effect of the enzyme alone and when it was a pretreatment. Malonate buffer concentration, MnP charge, and pH were the three factors used and they were varied between two levels, high and low. An analysis of the data indicate that both sequences raised the brightnesses of the two pulps. Following Q(MnP)QP bleaching, brightness reversion was reduced, pulp
산화적 스트레스(Oxidative Stress)는 Reactive Oxygen Species (ROS)와 antioxidant (항산화제)간의 불균형에 의해 발생되며, ROS의 과도한 축적은 DNA, 단백질 및 지질 세포막의 손상과 같은 세포적 손상을 초래합니다.. 산화적 스트레스의 조건에서 발생되는 Hydrogen Peroxide (H2O2)와 같은 과산화물(peroxide)은 ROS의 대표적인 산물 중 하나로 진핵세포에서는 유독하며, 높은 농도에서는 DNA, 지질 단백질의 산화를 일으켜 돌연변이 유발이나 세포사멸을 일으키기도 합니다.. peroxide에 의한 세포적 손상은 노화, 천식, 관절염, 당뇨병, 심혈관 질환, 신경성 퇴행성 질환 등 여러 질환의 발병과도 관계가 있습니다. EZ-Hydrogen Peroxide/Peroxidase assay kit는 Oxi-Probe를 사용하여 Hydrogen Peroxide (H2O2) 또는 Peroxidase 활성을 측정할 수 있는 제품으로 간단한 실험 방법과 민감도를 가지는 제품입니다. 본 Kit는 ...
Title: Recents Patents in the Use of Peroxidases. VOLUME: 3 ISSUE: 2. Author(s):Berenize Alvarado and Eduardo Torres. Affiliation:Benemerita Universidad Autonoma de Puebla, Centro de Quimica-ICUAP. Ciudad Universitaria, Puebla, 72570. Mexico.. Keywords:Biosensor, enzymatic oxidation, peroxidases, review. Abstract: Peroxidases are hemoenzymes with a wide range of applications, from fine chemical synthesis to environmental biocatalysis. These outstanding biocatalysts are able to catalyze reactions such as heteroatom oxidation (Nand S-oxidation), epoxidation, hydroxylation, and the oxidation of alcohols and indole, often giving high yields and enantiomeric excess values. This makes these biocatalysts very useful for application to several biotechnological processes. In this paper, recent advances and patents surrounding the use of peroxidases are reviewed, covering different aspects related to the applications of peroxidases and the modifications carried out to improve their functionality as ...
casSAR Dugability of Q73WB6 | MAP_2744c | Catalase-related peroxidase - Also known as CRPE_MYCPA, MAP_2744c. Has an organic peroxide-dependent peroxidase activity. Exhibits strong peroxidase activity using organic hydroperoxides as cosubstrates, weak peroxidase activity using hydrogen peroxide and negligible catalase activity. May have a role in elimination of reactive oxygen species, in particular by deactivating hydroperoxides. Monomer.
There is another endogenous peroxidase blocking method the blocks peroxidases and pseudoperoxidases. It can be used for minimally fixed frozen sections and in your case, paraffin sections. The is very thorough and gentle. It is called Glucose Oxidase blocking method. We love it for frozens as it does not chew sections off the slide. We have not tried it on paraffin but I have it by private communication it will work with paraffin sections/NBF fixed. I have seen it reported as 30 min in the working solution but original publication used it for 60 min. I will be happy to attach the method to you, not via Histonet. At 09:21 PM 6/23/2004 -0400, you wrote: >Todd, > Youre right. Sorry I havent responded to everyones suggestions >on my posting on blocking endogenous peroxidase in paraffin >sections. I think those who suggested that biotin might be the >problem have a point, however when I ran control sections with >just buffer, blocking (10% goat serum), and DAB, I still got >brown, even though ...
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Hamster mast cells have been found to give strong peroxidatic reactions at pH 5, 7.5 and 10 when sections of skeletal muscle are incubated for 2.5 h in the dark at room temperature on semipermeable...
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Enzyme Explorer - Peroxidase Enzymes products. Sigma-Aldrichs peroxidase product is recognized around the world as the industry standard for diagnostic manufacturing and laboratory scale research applications.
DAB (3, 3 -diaminobenzidine) HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications. DAB chromogen is effective as a single label or as a second color for multiple antigen labeling.
DAB (3, 3 -diaminobenzidine) HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications.
1. Thaw plates before opening bags to prevent condensation in the wells. 2. Add 200ul Blocking Buffer to each well and incubate 1 hour @ room temperature (RT). 3. Wash 3x w/ PBST. Aspirate and blot dry. 4. Prepare standards and samples in PBS. 5. Add 100ul of dilutions to wells. 6. Incubate plate 2-3h @ RT. 7. Incubate o/n @ 4C. 8. Wash 3x w/ PBST. Aspirate and blot dry. 9. Dilute biotinylated mAB211 in PBST + 0.5% Bovine Serum Albumin (BSA). 10. Add 100ul diluted Ab to each well. Incubate 2h @ RT. 11. Wash 3x w/ PBST. Aspirate and blot dry. 12. Prepare HRP-Streptavidin...... 13. Add 100ul diluted HRP-Streptavidin to each well. Incubate 2h @ RT. 14. Wash 3x w/ PBST. Aspirate and blot dry. 15. Prepare Peroxidase Substrate immediately before use. 16. Add 100ul Peroxidase Substrate to each well and incubate 10min in the dark @ RT. 17. Add 100ul Stopper Solution to wells and read plate at 490nm. ...
peroxidase, putative; FUNCTIONS IN: electron carrier activity, peroxidase activity, heme binding; INVOLVED IN: response to oxidative stress; LOCATED IN: endomembrane system; EXPRESSED IN: 10 plant structures; EXPRESSED DURING: 6 growth stages; CONTAINS InterPro DOMAIN/s: Haem peroxidase (InterPro:IPR010255), Plant peroxidase (InterPro:IPR000823), Haem peroxidase, plant/fungal/bacterial (InterPro:IPR002016); BEST Arabidopsis thaliana protein match is: peroxidase, putative (TAIR:AT5G58400.1); Has 2891 Blast hits to 2875 proteins in 212 species: Archae - 0; Bacteria - 4; Metazoa - 2; Fungi - 66; Plants - 2777; Viruses - 0; Other Eukaryotes - 42 (source: NCBI BLink ...
Streptococcus pneumoniae is a facultative anaerobic pathogen. Although it maintains fermentative metabolism, during aerobic growth pneumococci produce high levels of H2O2, which can have adverse effects on cell viability and DNA, and influence pneumococcal interaction with its host. The pneumococcus is unusual in its dealing with toxic reactive oxygen species in that it neither has catalase nor the global regulators of peroxide stress resistance. Previously, we identified pneumococcal thiol peroxidase (TpxD) as the key enzyme for enzymatic removal of H2O2, and showed that TpxD synthesis is up-regulated upon exposure to H2O2. This study aimed to reveal the mechanism controlling TpxD expression under H2O2 stress. We hypothesize that H2O2 activates a transcription factor which in turn up-regulates tpxD expression. Microarray analysis revealed a pneumococcal global transcriptional response to H2O2. Mutation of tpxD abolished H2O2-mediated response to high H2O2 levels, signifying the need for an active TpxD
Peroxidase Conjugated Affinity Purified Anti-SHEEP IgG F(ab)2 (RABBIT), Peroxidase Conjugated Affinity Purified anti-Sheep IgG F(ab )2 [Rabbit]; N/A Peroxidase Conjugated Affinity Purified Anti-SHEEP IgG F(ab)2 (RABBIT)IGHG1
cis-regulatory elements of the peroxidase gene in Arabidopsis thaliana involved in root-specific expression and responsiveness to high-salt stress.: The pattern
The Determination of Peroxidase in Amniotic Fluid.: Analyzing total peroxidase activity in amniotic fluid is extremely simple, requiring only 1 1/2 minutes of i
Within biological systems iron is a transition metal that allows access to the benefits of molecular oxygen as an oxidant. However, with these benefits come grave consequences if the reactions are not strictly controlled. The most prominent strategy of control and specialization is the protein environment that surrounds iron. Within iron containing proteins, specifically heme proteins, there are four basic levels of structure that impact the irons function: cofactor structure, protein-supplied ligands, non-ligand active site environment, and protein features that are distant from the active site. This last level remains poorly understood due to a lack of good models to pursue such studies. Catalase-peroxidases are unique heme proteins because they catalyze peroxide decomposition by two separate mechanisms, catalase and peroxidase, using the same active site. However, were it not for three structural features distant from the active site, catalase-peroxidases would be practically superimposable ...
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SWISS-MODEL Template Library (SMTL) entry for 1u2l. Crystal structure of the C-terminal domain from the catalase-peroxidase KatG of Escherichia coli (P1)
1NHQ: Crystallographic analyses of NADH peroxidase Cys42Ala and Cys42Ser mutants: active site structures, mechanistic implications, and an unusual environment of Arg 303.
Purified Native R. communis Lectin Toxin RCA60, Peroxidase labeled from Creative Biomart. Native R. communis Lectin Toxin RCA60, Peroxidase labeled can be used for research.
1NHP: Crystallographic analyses of NADH peroxidase Cys42Ala and Cys42Ser mutants: active site structures, mechanistic implications, and an unusual environment of Arg 303.
в сборнике Proceeding. Plant Peroxidase. Biochem. and Physiology. IY Int. Symp, место издания Ed. C.Obinger, U.Burner, R.Ebermann, C.Penel, H.Greppin, University of Agriculture, Vienna, and University of Geneva, с. 198-203 ...
is this on human tissues? abizar 1-877-igx-info -----Original Message----- From: [email protected] [mailto:[email protected]] Sent: Wednesday, May 03, 2000 3:22 PM To: [email protected] Subject: controls for rabbit polyclonals Plea for help, I am unable to get clean control slides for any rabbit polyclonal antibodies on either paraffin or frozen sections. There is always background (often very dark) on the collagen, smooth muscle (especially prostate) etc. This happens with commercial antibodies (B2microglobulin or vonWillebrand), in house Abs, as well as with rabbit IgG. I use casein or superblock. Avidin-Biotin block and exhaust endogenous peroxidase. I use the ABC kit with DAB. There is no problem if I omit the primary antibody. Can anybody offer any insights? Thank you, Bonnie ...
Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Sodium atom in PDB 2b2r: Crystal Structure Of An Oxoferryl Species of Catalase- Peroxidase Katg At PH5.6
peroxidase: Any of a group of enzymes that occur especially in plant cells and catalyze the oxidation of a substance by a peroxide.
anti-Glutathione Peroxidase 7, Polyclonal, Novus Biologicals 0.1mL; Unlabeled Life Sciences:Antibodies:Primary Antibodies:Immunoprecipitation (IP)
Histofine Simple Stain MAX Peroxidase (Human Tissue, Mouse primary): 414131F by As One International, Inc. at - Read reviews, citations, datasheets, protocols & more.
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Click on a taxon to see the following taxons, if there are any.. By placing the mouse over a node a tooltip will appear. This tootip contains the taxonomic path from Cellular organisms to the node and, in the case of a species, the number of peroxidases in the orthogroup, the total number of peroxidases for that species in the orthogroups class and where possible the names of those peroxidases. The common ancestor for the orthogroup is written in bold in the tooltip. Return to orthogroup ...
The orthogroup DiHCcP001 is composed of 84 sequences in 68 species. Click here to see a graphical representation of the species in this orthogroup.. ...
A cDNA for thylakoid-bound ascorbate peroxidase of pumpkin was cloned and characterized. Thylakoid-bound ascorbate peroxidase had a high similarity to cytosolic ascorbate peroxidases, and the precursor contained a transit peptide to chloroplasts at its ammo-terminus and a putative membrane-spanning region at its carboxy-terminus.. ...
The ionic strength dependence of the reduction of cytochrome c peroxidase compound I by horse cytochrome c has been studied, using stopped-flow technigue, in pH 7.5, potassium phosphate/nitrate buffer. The temperature was set at 25 ± 1° C. The wavelength was monitored at 4 32 nm, an isobestic point for ferri-/ferrocytochrome c, so only the absorbance change of the reduction of cytochrome c peroxidase compound I to compound II can be observed. The observed rate constant, kobs, as a function of the concentration of ferrocytochrome c shows a non-linear increase with increasing ionic strength. A two-parameter eguation is needed to fit the data at low ionic strength, 20 mM to 40 mM, while a three-parameter equation is needed at high ionic strength, 65 mM and above. The maximum rates of these reductions also show two different types of ionic strength dependence. At 20 mM to 40 mM ionic strength, the maximum rate of reduction decreases slightly, within experimental error, with increasing ionic ...
We previously demonstrated that manganese peroxidase from the white-rot fungus Phanerochaete chrysosporium was very susceptible to thermal inactivation due to the loss of calcium from the enzyme [Sutherland & Aust (1996) Arch. Biochem. Biophys. 332, 128-134]. The structural changes that occur during thermal inactivation and the release of calcium from manganese peroxidase have now been characterized. Thermal inactivation caused distinct alterations in the heme environment and slight changes in the overall protein structure, both of which were reversed upon reactivation of the enzyme with calcium. The absorption spectrum of inactivated manganese peroxidase was similar to that of low-spin ferric heme proteins, indicating that a sixth ligand had bound to the distal side of the heme iron. Consistent with disruption of the distal heme environment, thermally inactivated manganese peroxidase did not react with hydrogen peroxide to form compound I. The inactive enzyme exhibited a pH-dependent absorption
TY - JOUR. T1 - Crystal structure of lignin peroxidase. AU - Edwards, Steven L.. AU - Raag, Reetta. AU - Wariishi, Hiroyuki. AU - Gold, Michael H.. AU - Poulos, Thomas L.. PY - 1993/1/15. Y1 - 1993/1/15. N2 - The crystal structure of lignin peroxidase (LiP) from the basidiomycete Phanerochaete chrysosporium has been determined to 2.6 Å resolution by using multiple isomorphous replacement methods and simulated annealing refinement. Of the 343 residues, residues 3-335 have been accounted for in the electron density map, including four disulfide bonds. The overall three-dimensional structure is very similar to the only other peroxidase in this group for which a high-resolution crystal structure is available, cytochrome c peroxidase, despite the fact that the sequence identity is only ≈20%, LiP has four disulfide bonds, while cytochrome c peroxidase has none, and LiP is larger (343 vs. 294 residues). The basic helical fold and connectivity defined by 11 helical segments with the heme sandwiched ...
Species of the genus Pleurotus are among the most efficient natural species in lignin degradation belonging to the subclass of ligninolytic organisms that produce laccase (Lac), Mn-dependent peroxidase (MnP), versatile peroxidase (VP), and the H2O2-generating enzyme aryl-alcohol oxidase, but not lignin peroxidases. Production of Lac and oxidation of 2,6-dimethoxyphenol (DMP) in the presence and absence of Mn2+ were detected both in submerged fermentation (SF) of dry ground mandarine peels and in solid-state fermentation (SSF) of grapevine sawdust in all investigated Pleurotus species and strains. Evidence of cultivation methods having a distinct influence on the level of enzyme activities has been demonstrated. Most of the species and strains had higher Lac activity under SSF conditions than under SF conditions. DMP oxidation in the presence and absence of Mn2+ was detected in all investigated species and strains, but was lower under SF conditions than under SSF conditions for most of ...them. ...
Additional file 19: of Genome-wide and evolutionary analysis of the class III peroxidase gene family in wheat and Aegilops tauschii reveals that some members are involved in stress responses
Author(s): Smith, Martyn T.; Yager, J; Steinmetz, K; Eastmond, D | Abstract: The metabolism of two of benzenes phenolic metabolites, phenol and hydroquinone, by peroxidase enzymes has been studied in detail. Studies employing horseradish peroxidase and human myeloperoxidase have shown that in the presence of hydrogen peroxide phenol is converted to 4,4-diphenoquinone and other covalent binding metabolites, whereas hydroquinone is converted solely to 1,4-benzoquinone. Surprisingly, phenol stimulates the latter conversion rather than inhibiting it, an effect that may play a role in the in vivo myelotoxicity of benzene. Indeed, repeated coadministration of phenol and hydroquinone to B6C3F1 mice results in a dramatic and significant decrease in bone marrow cellularity similar to that observed following benzene exposure. A mechanism of benzene-induced myelotoxicity is therefore proposed in which the accumulation and interaction of phenol and hydroquinone in the bone marrow and the peroxidase-dependent
Mitochondrial swelling and membrane protein thiol oxidation associated with mitochondrial permeability transition induced by Ca2+ and inorganic phosphate are inhibited in a dose-dependent manner either by catalase, the thiol-specific antioxidant enzyme (TSA), a protein recently demonstrated to present thiol peroxidase activity, or ebselen, a selenium-containing heterocycle which also possesses thiol peroxidase activity. This inhibition of mitochondrial permeability transition is due to the removal of mitochondrial-generated H2O2, which can easily diffuse to the extramitochondrial space. Whereas ebselen required the presence of reduced glutathione as a reductant to grant its protective effect, TSA was fully reduced by mitochondrial components. Decrease in the oxygen concentration of the reaction medium also inhibits mitochondrial permeabilization and membrane protein thioloxidation, in a concentration-dependent manner. The results presented in this report confirm that mitochondrial permeability ...
Poole, L.B., and Ellis, H.R. (1996) Flavin-dependent alkyl hydroperoxide reductase from Salmonella typhimurium. 1. Purification and enzymatic activities of overexpressed AhpF and AhpC proteins. Biochemistry 35, 56-64. PDF of article Poole, L.B. (1996) Flavin-dependent alkyl hydroperoxide reductase from Salmonella typhimurium. 2. Cystine disulfides involved in catalysis of peroxide reduction. Biochemistry 35, 65-75. PDF of article Poole, L.B., Chae, H.Z., Flores, B.M., Reed, S.L., Rhee, S.G., and Torian, B.E. (1997) Peroxidase activity of a TSA-like antioxidant protein from a pathogenic amoeba. Free Radical Biol. Med. 23, 955-959. PDF of article Li Calzi, M., and Poole, L.B. (1997) Requirement for the two AhpF cystine disulfide centers in catalysis of peroxide reduction by alkyl hydroperoxide reductase. Biochemistry 36, 13357-13364. PDF of article Ellis, H. R., and Poole, L.B. (1997) Roles for the two cysteine residues of AhpC in catalysis of peroxide reduction by alkyl hydroperoxide reductase ...
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Kangasjärvi, Saijaliisa; Lepistö, Anna; Hannikainen, Kati; Piippo, Mirva; Luomala, Eeva-Maria; Aro, Eva-Mari; Rintamäki, Eevi (2008 ...
Lactoperoxidase is a peroxidase enzyme secreted from mammary, salivary, and other mucosal glands that functions as a natural antibacterial agent. Lactoperoxidase is a member of the heme peroxidase family of enzymes. In humans, lactoperoxidase is encoded by the LPO gene. Lactoperoxidase catalyzes the oxidation of a number of inorganic and organic substrates by hydrogen peroxide. These substrates include bromide and iodide and therefore lactoperoxidase can be categorised as a haloperoxidase. Another important substrate is thiocyanate. The oxidized products produced through the action of this enzyme have potent bactericidal activities. Lactoperoxidase together with its inorganic ion substrates, hydrogen peroxide, and oxidized products is known as the lactoperoxidase system. The lactoperoxidase system plays an important role in the innate immune system by killing bacteria in milk and mucosal (linings of mostly endodermal origin, covered in epithelium, which are involved in absorption and secretion) ...
en] The cloning and sequencing of the rbpa gene coding for a versatile peroxidase from a novel Bjerkandera strain is hereby reported. The 1777 bp isolated fragment contained a 1698 bp peroxidase-encoding gene, interrupted by 11 introns. The 367 amino acid-deduced sequence includes a 27 amino acid-signal peptide. The molecular model, built via homology modelling with crystal structures of four fungal peroxidases, highlighted the amino acid residues putatively involved in manganese binding and aromatic substrate oxidation. The potential heme pocket residues (R44, F47, H48, E79, N85, H177, F194 and D239) include both distal and proximal histidines (H48 and H177). RBP possesses potential calcium-binding residues (D49, G67, D69, S71, S178, D195, T197, I200 and D202) and eight cysteine residues (C3, C15, C16, C35, C121, C250, C286, C316). In addition, RIBP includes residues involved in substrate oxidation: three acidic residues (E37, E41 and D183)-putatively involved in manganese binding and H83 and ...
A picture of substrate binding for small, aromatic substrates at the δ-heme edge emerged largely from chemical modification work (reviewed in [5,6,14,15]), although NMR also played an important role [16-23]. A few structures appeared at the end of the 1990s for horseradish peroxidase and Arthromyces ramosus peroxidase in complex with various small organic molecules [1-3], showing binding at the δ-heme edge, and there is now a collection of other crystal structures in which binding of various organic compounds has been observed at the same site (not all of which are genuine substrates) [1-3,12,13,24-27]. For CcP, there was a general consensus of opinion that small substrates were bound close to the δ-site [7,28-30]. The actual binding site for guaiacol has never been identified, although there is a report of phenol binding to an artificially created cavity in CcP [31].. In our structure, we observed guaiacol binding at two sites, close to Phe68 and Ile40. Guaiacol was not seen bound at the ...
TY - JOUR. T1 - Unfolding and pH studies on manganese peroxidase. T2 - Role of heme and calcium on secondary structure stability. AU - Banci, Lucia. AU - Bartalesi, Ilaria. AU - Ciofi-Baffoni, Simone. AU - Tien, Ming. PY - 2003/1/25. Y1 - 2003/1/25. N2 - The present study characterizes the unfolding and folding processes of recombinant manganese peroxidase. This enzyme contains five disulfide bonds, two calcium ions, and one heme prosthetic group. Circular dichroism in the far UV was used to monitor global changes of the protein secondary structure, whereas UV-visible spectroscopy of the Soret band provided information about local changes in the heme cavity. The effects of reducing agents, oxidizing agents, and denaturants on this process were investigated. In addition to affecting the secondary structure content, these factors also affect the binding of the heme and the calcium ions, both of which have a significant effect on the folding process. Our results also show that denaturants induce ...
Endogenous peroxidase activity has been demonstrated in sections of rat liver fixed briefly by glutaraldehyde perfusion and incubated in Graham and Karnovskys medium for cytochemical demonstration of peroxidase activity (29). In 25-40% of sinusoidal cells, an electron-opaque reaction product is localized in segments of the endoplasmic reticulum, including the perinuclear cisternae, a few Golgi vesicles and saccules and in some large membrane-bounded granules. This staining is abolished after prolonged fixation or boiling of tissue sections in glutaraldehyde, and in the absence of H2O2 or DAB from the incubation medium. Furthermore, the reaction is inhibited completely by sodium azide and high concentrations of H2O2, and partially by KCN and aminotriazole. Among the different cells in hepatic sinusoids, the nonphagocytic fat-storing cells (39) are always peroxidase negative, whereas the lining cells in process of erythrophagocytosis are consistently peroxidase positive. The possible biological ...
The plasma membrane-bound peroxidases (PRX) zmprx01, zmprx66 and zmprx70 and the respiratory burst oxidase homologs (RBOH) rbohA, rbohB, rbohC and rbohD were analysed in this study. The distribution of the genes inside the roots was investigated by real-time qPCR. Therefor four different segments (root tip, elongation zone, differentiation zone and lateral roots) were in focus of the analyses. It could be observed that the genes are differently distributed in the root. The peroxidases were predominantly expressed in the elongation zone and almost not in the root tip. The rboh genes were more inhomogeneous distributed. For each RBOH a specific expression pattern could be detected. rbohA was mostly expressed in the differentiation zone. rbohB was more even expressed in the root. rbohC was even distributed as well but predominantly in the elongation zone. rbohD was mostly expressed in the differentiation zone. For a further investigation of the peroxidases plants were exposed to cadmium (short term and
Reactive oxygen species (ROS), such as O2 and H2O2, play a key role in plant metabolism, cellular signaling, and defense. In leaf cells, the chloroplast is considered to be a focal point of ROS metabolism. It is a major producer of O2 and H2O2 during photosynthesis, and it contains a large array of ROS-scavenging mechanisms that have been extensively studied. By contrast, the function of the cytosolic ROS-scavenging mechanisms of leaf cells is largely unknown. In this study, we demonstrate that in the absence of the cytosolic H2O2-scavenging enzyme ascorbate peroxidase 1 (APX1), the entire chloroplastic H2O2-scavenging system of Arabidopsis thaliana collapses, H2O2 levels increase, and protein oxidation occurs. We further identify specific proteins oxidized in APX1-deficient plants and characterize the signaling events that ensue in knockout-Apx1 plants in response to a moderate level of light stress. Using a dominant-negative approach, we demonstrate that heat shock transcription factors play a ...
A dye-decolorizing peroxidase (DyP) from Pleurotus ostreatus (PosDyP4) catalyzes the oxidation of Mn2+ to Mn3+, in the presence of H2O2, with an efficiency similar to the well-known manganese peroxidases and versatile peroxidases from this and other white-rot fungi. PosDyP4 has been overexpressed in Escherichia coli as an active enzyme, and its crystal structure has been solved at 1.56 Å resolution. A combination of substrate diffusion simulations on the solved structure using the PELE software, electron paramagnetic resonance, and site-directed mutagenesis led to identification of the residues involved in Mn2+ oxidation. The oxidation site in PosDyP4 is different than the conserved site in the other Mn-oxidizing peroxidases mentioned above, and it includes four acidic residues (three aspartates and one glutamate) located at the surface of the protein. Moreover, since the Mn2+ ion is not in direct contact with the heme propionates, a tyrosine residue participates in the electron transfer to the ...
The basidiomycete isolate b19, originally identified by morphological characteristics of the fruiting body as Nematoloma frowardii, efficiently produces manganese peroxidase (MNP) and is used for degradation of natural, persistent aromatic polymers (lignin, humic acids and brown coal components). The N. frowardii MNP has shown good activity in conversion of xenobiotic compounds such as polycyclic hydrocarbons and trinitrotoluene. However, this biotechnologically promising fungus has not previously been studied at the molecular biology level. We show here that according to the molecular characterization of its main MNP isozyme, Nf b19 MNP2, and partial sequencing of its MNP3-, three lignin peroxidase- and two laccase-encoding genes, and the gene encoding the ribosomal SSU 18S RNA, that the fungus has a close phylogenetic relationship to the white-rot basidiomycete Phlebia radiata (Fr.). Ribosomal internal transcribed spacer (ITS) sequence (ITS1+5.8S+ITS2) phylogeny reclassifies Nf b19 as a possible
TY - JOUR. T1 - Metal free MoS2 2D sheets as a peroxidase enzyme and visible-light-induced photocatalyst towards detection and reduction of Cr(vi) ions. AU - Borthakur, Priyakshree. AU - Boruah, Purna K.. AU - Das, Manash R.. AU - Artemkina, Sofya B.. AU - Poltarak, Pavel A.. AU - Fedorov, Vladimir E.. PY - 2018/10/21. Y1 - 2018/10/21. N2 - Two-dimensional molybdenum disulphide (MoS2) sheets were prepared by using simple thermal decomposition method. The synthesized sheets were characterized by different analytical tools including FTIR, XRD, XPS, Raman spectroscopy, HRTEM and FESEM analyses. The synthesized sheets exhibited outstanding peroxidase mimicking activity towards the peroxidase oxidation of chromogenic probe molecule 3,3′,5,5′-tetramethyl benzidine (TMB), producing a blue coloured solution of 3,3′,5,5′-tetramethylbenzidine diimine (TMBDI) in an acidic medium (pH 4). Based on this peroxidase mimicking activity it was utilized for colorimetric determination of toxic Cr(vi) ions ...
In this study, we sought to determine whether the increases in peroxidase activity and electrolyte leakage induced in maize (Zea mays L.) leaves by sodium bisulfite were causally related to the sodium bisulfite-induced increases in sporulation of the pathogen Bipolaris maydis race T on infected maize leaves. Pretreatment of detached leaves of maize inbred W64 A with sodium bisulfite (500 ,ig/ml) for 24 h in the dark at 28°C increased peroxidase activity in the Tms cytoplasm (susceptible) isoline compared with the N cytoplasm (resistant) isoline. No such differences in peroxidase activity between the two isolines were observed when detached leaves were pretreated with double distilled water. The sodium bisulfite-induced increase in peroxidase activity persisted even when leaves pretreated with sodium bisulfite were inoculated with R maydis race T and subsequently incubated for 48 h in the dark at 28° C. Similarly, pretreatment with sodium bisulfite caused a greater increase in electrolyte ...
The ability of plant cells cultivated ,i,in vitro,/i, to metabolize polychlorinated biphenyls (PCBs) was correlated with the morphology of the cultures tested as models for phytoremediation studies. More differentiated cultures showed generally higher transformation capacity. The ability of plant cells to transform PCBs is connected to their viability in the presence of PCBs and their behaviour can be positively correlated with the production of intracellular and extracellular peroxidases. The cultures with high PCB-transforming activity proved to exhibit high peroxidase activity in the presence of PCBs while those with low ability to metabolize PCB showed a decrease of the enzyme activity in the presence of PCBs. Experiments with propylgallate were used to distinguish the ratio of involvement of peroxidases in PCB metabolism. ,p, ...
The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB.
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.
The surface oxidation site (Trp171) in lignin peroxidase (LiP) which is specific for the reaction with the high redox potential substrate, veratryl alcohol (1.4 V), had been previously engineered in a heme peroxidase that has similar protein fold but lacks this activity. The new Trp site was engineered by introducing a Trp residue in the coprinus cinereus peroxidase (CiP) at the equivalent position to that of the Trp171 in LiP. In order to induce the catalytic activity towards veratryl alcohol in CiP, it was also necessary to reproduce the naturally-occurring negatively-charged microenvironment of the Trp site. Multifrequency EPR spectroscopy characterization of the D179W+R258E+R272D variant of CiP unequivocally showed that, upon reaction of the enzyme with hydrogen peroxide a new Trp radical (g-values of gx = 2.0035(5), gy = 2.0027(5) and gz = 2.0022(1)) was formed after the [Fe(IV)=O Por+] intermediate, as a result of intramolecular electron transfer between Trp179 and the porphyrin. The EPR ...
Article Evaluation of manganese peroxidase (MnP) for its ability to resist the ozonization and thereafter decolorize methyl orange. The goal of this study was to determine whether ozone can be used to suppress bacterial growth in operating a white ro...
Suspension culture of compact callus aggregates (CCA) of Daucus carota (carrot), consisting of yellow, spherical cellular clumps displaying tissue differentiation was established. The production of peroxidase which was detected in trace amounts in dispersed carrot cell cultures was found in high amounts (5.0-14.5 U g(-1) FW) in CCA cultures. Kinetics analysis showed that CCA grew quickly for 4-12 days with a specific growth rate of 0.20 day(-1), while the peroxidase activity increased sharply after day 12. Fungal elicitors were found to effectively induce peroxidase synthesis. Peroxidase activity of 54.0 U g(-1) FW was obtained by adding Aspergillus niger elicitor at day 16 to make its concentration in medium 50 mg l(-1). (C) 1998 Elsevier Science B.V. All rights reserved.; Suspension culture of compact callus aggregates (CCA) of Daucus carota (carrot), consisting of yellow, spherical cellular clumps displaying tissue differentiation was established. The production of peroxidase which was ...
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The effect of NaCl on total peroxidase activity, induction of isoperoxidases and lipid peroxidation in 5-day-old seedlings of two contrasting genotypes of Setaria italica L. (Prasad, a salt tolerant cultivar and Lepakshi, a salt susceptible cultivar), was studied. Total peroxidase activity increased under NaCl salinity and the degree of elevation in the activity was salt concentration dependent. Nevertheless, a greater activity was recorded in the tolerant cultivar (cv Prasad) compared to the susceptible (cv Lepakshi) one in all days of sampling. Further, the pattern of isoperoxidases was modified during stress conditions as evident from the electrophoregrams. Although, five acidic isoforms were detected in both cultivars, differences were found between the cultivars. Furthermore, it was observed that acidic isoperoxidases were strongly expressed and an acidic isoperoxidase, A(3p) (27 kDa) is specifically found in the tolerant cultivar (cv Prasad) under NaCl stress. This isoform was partially ...
TY - JOUR. T1 - Structure-function studies of cytochrome c peroxidase from ps. nautica. AU - Alves, T.. AU - Besson, S.. AU - Pereira, A. S.. AU - Pettigrew, G. W.. AU - Moura, José J. G.. AU - Moura, I.. PY - 2001/8. Y1 - 2001/8. M3 - Meeting Abstract. VL - 86. SP - 122. EP - 122. JO - Journal of Inorganic Biochemistry. JF - Journal of Inorganic Biochemistry. SN - 0162-0134. IS - 1. ER - ...
297283835 - EP 0898620 B1 2002-11-06 - ASSAY OF PEROXIDASE ACTIVITY - [origin: WO9739142A1] Chemiluminescent detection of molecules of synthetic or natural origin such as proteins and nucleic acids (DNA and RNA), as well as other biologic molecules, is increasingly replacing radioactive detection as the method of choice where sensitivity is critical. In such assays, luminescence is customarily achieved by the oxidation of a luminol or isoluminol substrate in the presence of an oxidizing agent such as hydrogen peroxide or hydrogen peroxide source, such as perborate, and a peroxidase catalyst such as horseradish peroxidase. To obtain useful levels of luminescence (e.g., detectable levels) by customary techniques, a luminescent enhancer is also present during oxidation. It has been found in the practice of the present invention that azine enhancers have contained an impurity which reduces the properties of the chemiluminescent assay working solutions. The present invention describes a working solution, a
Cold acclimation in woody perennials is a metabolically intensive process, but coincides with environmental conditions that are not conducive to the generation of energy through photosynthesis. While the negative effects of low temperatures on the photosynthetic apparatus during winter have been well studied, less is known about how this is reflected at the level of gene and metabolite expression, nor how the plant generates primary metabolites needed for adaptive processes during autumn. The MapMan tool revealed enrichment of the expression of genes related to mitochondrial function, antioxidant and associated regulatory activity, while changes in metabolite levels over the time course were consistent with the gene expression patterns observed. Genes related to thylakoid function were down-regulated as expected, with the exception of plastid targeted specific antioxidant gene products such as thylakoid-bound ascorbate peroxidase, components of the reactive oxygen species scavenging cycle, and the
Cold acclimation in woody perennials is a metabolically intensive process, but coincides with environmental conditions that are not conducive to the generation of energy through photosynthesis. While the negative effects of low temperatures on the photosynthetic apparatus during winter have been well studied, less is known about how this is reflected at the level of gene and metabolite expression, nor how the plant generates primary metabolites needed for adaptive processes during autumn. The MapMan tool revealed enrichment of the expression of genes related to mitochondrial function, antioxidant and associated regulatory activity, while changes in metabolite levels over the time course were consistent with the gene expression patterns observed. Genes related to thylakoid function were down-regulated as expected, with the exception of plastid targeted specific antioxidant gene products such as thylakoid-bound ascorbate peroxidase, components of the reactive oxygen species scavenging cycle, and the
Catalase-peroxidases are bifunctional enzymes that catalyze the removal of hydrogen peroxide by two distinct pathways (catalase and peroxidase). They are central to antibiotic resistance in Mycobacterium tuberculosis and may be virulence factors in several dangerous human pathogens. These enzymes also hold much promise for engineering new enzymes to combat long-standing problems (e.g., environmental contamination by toxic pollutants). Currently, understanding of catalase-peroxidase structure and function is lacking to facilitate new drug development and enzyme engineering. The purpose of the research described in this dissertation is to understand the molecular basis for the unique catalytic abilities of catalase-peroxidases to fully capitalize on their potential. The versatile catalytic abilities of these enzymes arise from an active site that is normally restricted to one activity (i.e., peroxidase). This is facilitated by three structures which are quite distant from the active site: a ...
peroxidase and IAA oxidase activities. Protein concentration was determined by the method of Bradford (1976) using bovine serum albumin (BSA) as protein standard.. IAA oxidase assay IAA oxidase activity was measured by the spectrophotometric method described by Beffa et al. (1990). Reaction mixtures contained 0.76 ml of 50 mM phosphate buffer (KH2PO4/Na2HPO4, pH 6.0), 0.01 ml of 5 mM MnCl2, 0.01 ml of 5 mM 2,4-dichlorophenol (DCP), 0.02 ml of 14.3 mM IAA and 0.2 ml of enzyme extract. The final volume of the reaction mixtures was 1 ml. Assays were conducted at 25.0 0.5 C for 1 h. Salkowski reagent was used to determine IAA destruction at the wavelength of 535 nm after 1 h. One unit of IAA oxidase activity is equivalent to a DA535 of 1.0 for 1 mg of protein in 1 h. Each value represented the mean of three replicates.. Peroxidase assay Peroxidase (EC activity was determined spectrophotometrically by measuring the increase in absorbance at 470 nm after 30 min incubation at 30.0 0.5 C ...
The report gives the research-based overview of on Global Horseradish Peroxidase (HRP) Market 2019 size, industry status and forecast, competition landscape and growth opportunity. This research report categorizes the global horseradish peroxidase (hrp) market by companies, region, type and end-use industry. It als...
Activity of ascorbate peroxidase (APX) (A), glutathione reductase (GR) (B), dehydroascorbate reductase (DR) (C), and monodehydroascorbate reductase (MR) (D) in
Anti-Mouse IgG3 (Gamma 3 chain) (Peroxidase Conjugated) Secondary Antibody, Rabbit Polyclonal, Peroxidase (Horseradish) validated in WB, E, IC (ASR3057), Abgent
Anti-Human IgG (gamma chain) (Peroxidase Conjugated) Secondary Antibody, Rabbit Polyclonal, Peroxidase (Horseradish) validated in WB, E, IC (ASR3050), Abgent
GPX1 encodes a member of the glutathione peroxidase family, which contains important antioxidant enzymes and helps detoxify hydrogen peroxide with glutathione, and thereby protect cells against oxidative damage (R). It protects the hemoglobin in red blood cells from oxidative breakdown.. H2O2 is also essential for growth-factor mediated signal transduction, mitochondrial function, and maintenance of antioxidant balance; therefore, by limiting H2O2 accumulation, glutathione peroxidases are also involved in modulating these processes.. It is also a selenoprotein, requiring selenium to function.. Associated with risk for breast cancer (R). ...
... catalyzed by the presence of a peroxidase (such as horseradish peroxidase). The hydrogen peroxide is itself produced by an ... "Peroxidases". In Ashok Pandey; Colin Webb; Carlos Ricardo Soccol; Christian Larroche (eds.). Enzyme technology. Springer. p. ...
Banerjee, R. K.; Bose, A. K.; Chakraborty, T. K.; De, S. K.; Datta, A. G. (1985). "Peroxidase-catalysed iodotyrosine formation ... Banerjee, R. K.; Datta, A. G. (1986). "Salivary peroxidases". Mol Cell Biochem. 70 (1): 21-29. doi:10.1007/bf00233801. PMID ... lysozyme and peroxidase. It has not been shown that human licking of wounds disinfects them, but licking is likely to help ...
Dunford BH (1999). Heme peroxidases. John Wiley. ISBN 0-471-24244-6. Dawson JH (April 1988). "Probing structure-function ... as exemplified by peroxidases, catalases, reductases, etc.) are ubiquitous in cellular systems. While several moiety and ... relations in heme-containing oxygenases and peroxidases". Science. 240 (4851): 433-439. Bibcode:1988Sci...240..433D. doi: ...
Enzyme kinetics Glutathione peroxidase Peroxidase Superoxide dismutase GRCh38: Ensembl release 89: ENSG00000121691 - Ensembl, ... "PeroxiBase - The peroxidase database". Swiss Institute of Bioinformatics. Archived from the original on 2008-10-13. Retrieved ... Maehly AC, Chance B (1954). "The assay of catalases and peroxidases". Methods of Biochemical Analysis. Methods of Biochemical ... contains catalases and peroxidases. To activate the noxious spray, the beetle mixes the contents of the two compartments, ...
... produces chitinase and peroxidase. Wang, W; Mai-Gisondi, G; Stogios, PJ; Kaur, A; Xu, X; Cui, H; ... Iqbal, M.; Mercer, D. K.; Miller, P. G. G.; McCarthy, A. J. (1 June 1994). "Thermostable extracellular peroxidases from ...
... thyroid peroxidase activity; and hormone release. TSHR is involved in regulating seasonal reproduction in vertebrates. Graves' ...
The research on these lignin-degrading enzymes produced by Bjerkandera adusta, such as versatile peroxidase, has also shown in ... ISBN 978-0-88192-935-5. Singh, R.; Eltis, L.D. (2015). "The multihued palette of dye-decolorizing peroxidases". Archives of ... "Lignin-degrading peroxidases in Polyporales: an evolutionary survey based on 10 sequenced genomes". Mycologia. 105 (6): 1428- ... "Functional Characterization of Melanin Decolorizing Extracellular Peroxidase of Bjerkandera adusta". Journal of Fungi. 7 (9): ...
There are at least four different glutathione peroxidase isozymes in animals. Glutathione peroxidase 1 is the most abundant and ... Glutathione peroxidase is an enzyme containing four selenium-cofactors that catalyzes the breakdown of hydrogen peroxide and ... Surprisingly, glutathione peroxidase 1 is dispensable, as mice lacking this enzyme have normal lifespans, but they are ... In addition to its direct antioxidant effects, ascorbic acid is also a substrate for the redox enzyme ascorbate peroxidase, a ...
Peroxidases are also involved for some species. The body of the prey is decomposed by a cocktail of hydrolytic enzymes which ...
Bacteria do not express any of the plant-type peroxidases (lignin peroxidase, Mn peroxidase, or versatile peroxidases), but ... Some ligninolytic enzymes include heme peroxidases such as lignin peroxidases, manganese peroxidases, versatile peroxidases, ... Lignin peroxidases oxidize non-phenolic lignin, whereas manganese peroxidases only oxidize the phenolic structures. Dye- ... Both peroxidase and laccase enzymes are present in the plant cell walls, and it is not known whether one or both of these ...
It was first limited to class III peroxidases (plant peroxidases) and was then expanded to include all possible haem and non- ... The majority of haem and non-haem peroxidase sequences can now be found in the PeroxiBase. The database is hosted by the Swiss ... Peroxidase sequences come from other general public databases (NCBI, TIGR, UniProt KnowledgeBase: all the databases used are ... haem peroxidase protein sequences. Many researchers and bioinformaticians from the University of Geneva joined their efforts to ...
PRSS7 Eosinophil peroxidase deficiency; 261500; EPX Epidermodysplasia verruciformis; 226400; TMC6 Epidermodysplasia ...
... eosinophil peroxidase (EPO), thyroid peroxidase (TPO), and prostaglandin H synthase (PGHS). A heme cofactor is bound near the ... Lactoperoxidase is a peroxidase enzyme secreted from mammary, salivary and other mucosal glands including the lungs, bronchii ... Peroxidase-generated hypoiodous acid (HOI), hypoiodite and hypothiocyanite all destroy the herpes simplex virus and human ... Lactoperoxidase is a member of the heme peroxidase family of enzymes. In humans, lactoperoxidase is encoded by the LPO gene. ...
These systems include peroxidases, catalases, and superoxide dismutases. A complementary metalloprotein to those that react ...
ISBN 978-1-947988-83-5. India portal Biology portal Medicine portal Lactoferrin Haem peroxidase Lactoperoxidase Please see ... "Structural basis of activation of mammalian heme peroxidases". Progress in Biophysics and Molecular Biology. 133: 49-55. doi: ...
Anti-thyroid peroxidase (anti-TPO) antibodies are specific for the autoantigen TPO, a 105kDa glycoprotein that catalyses iodine ... McLachlan SM, Rapoport B (2000). "Autoimmune response to the thyroid in humans: thyroid peroxidase--the common autoantigenic ... The most clinically relevant anti-thyroid autoantibodies are anti-thyroid peroxidase antibodies (anti-TPO antibodies, TPOAb), ... Taurog A (May 1999). "Molecular evolution of thyroid peroxidase". Biochimie. 81 (5): 557-62. doi:10.1016/S0300-9084(99)80110-2 ...
Glutathione peroxidase activity of inorganic selenium and seleno-DL-cysteine. Experientia 31: 769-770, 1975 Beutler E, et al. ... Electrophoretic polymorphism of glutathione peroxidase. Ann Hum Genet 38: 163-169, 1974 Beutler E, et al. ...
The enzyme is thyroid peroxidase. The small amount of T3 could be important because different tissues have different ...
... l is one important characteristic of animal peroxidases; plant peroxidases incorporate heme B. Lactoperoxidase and ... In peroxidase reactions, the porphyrin molecule also serves as an electron source, being able to delocalize radical electrons ... Heme m contains the two ester bonds at the heme 1- and 5-methyl groups also present in heme l of other mammalian peroxidases, ... In general, diatomic gases only bind to the reduced heme, as ferrous Fe(II) while most peroxidases cycle between Fe(III) and Fe ...
Some bacteria have NADH-dependent peroxidases specific for H2O2. The main nonenzymatic antioxidants in E. coli are GSH and ...
White WE, Pruitt KM, Mansson-Rahemtulla B (February 1983). "Peroxidase-Thiocyanate-Peroxide Antibacterial System Does Not ... Hypothiocyanite is formed by peroxidase catalysis of hydrogen peroxide and thiocyanate: H2O2 + SCN− → OSCN− + H2O ... "Active site structure and catalytic mechanisms of human peroxidases". Arch. Biochem. Biophys. 445 (2): 199-213. doi:10.1016/j. ... respiratory syncytial virus and echovirus type 11 by peroxidase-generated hypothiocyanite". Antiviral Res. 26 (2): 161-71. doi: ...
Here, trypanothione-dependent enzymes such as tryparedoxin peroxidase (TryP) reduce peroxides using electrons donated either ... Trypanothione-dependent enzymes include reductases, peroxidases, glyoxalases and transferases. Trypanothione-disulfide ...
"New and classic families of secreted fungal heme peroxidases". Appl Microbiol Biotechnol. 87 (3): 871-897. doi:10.1007/s00253- ...
"Proximity-based protein thiol oxidation by H2O2-scavenging peroxidases". The Journal of Biological Chemistry. 284 (46): 31532- ...
Norisoprenoids, such as C13-norisoprenoids found in grape (Vitis vinifera) or wine, can be produced by fungal peroxidases or ... "Generation of Norisoprenoid Flavors from Carotenoids by Fungal Peroxidases". Journal of Agricultural and Food Chemistry. 57 (21 ...
... can be produced by fungal peroxidases or glycosidases. While terpenes and terpenoids occur widely, their extraction from ... "Generation of Norisoprenoid Flavors from Carotenoids by Fungal Peroxidases". Journal of Agricultural and Food Chemistry. 57 (21 ...
Glutathione peroxidase 3 (GPx-3), also known as plasma glutathione peroxidase (GPx-P) or extracellular glutathione peroxidase ... Lin JC, Kuo WR, Chiang FY, Hsiao PJ, Lee KW, Wu CW, Juo SH (May 2009). "Glutathione peroxidase 3 gene polymorphisms and risk of ... "Entrez Gene: glutathione peroxidase 3 (plasma)". Takahashi K, Avissar N, Whitin J, Cohen H (August 1987). "Purification and ... Saga Y, Ohwada M, Suzuki M, Konno R, Kigawa J, Ueno S, Mano H (December 2008). "Glutathione peroxidase 3 is a candidate ...
Glutathione peroxidase 7 is an enzyme that in humans is encoded by the GPX7 gene. GRCh38: Ensembl release 89: ENSG00000116157 ... PDBe-KB provides an overview of all the structure information available in the PDB for Human Glutathione peroxidase 7 Portal: ... "Entrez Gene: GPX7 glutathione peroxidase 7". Opalenik SR, Ding Q, Mallery SR, Thompson JA (1998). "Glutathione depletion ... 2001). "Human immunodeficiency virus type 1 Tat protein impairs selenoglutathione peroxidase expression and activity by a ...
Glutathione peroxidase 8 (GPx-8) is an enzyme that in humans is encoded by the GPX8 gene. GPx-8 is a member of the glutathione ... "Entrez Gene: glutathione peroxidase 8 (putative)". Toppo S, Vanin S, Bosello V, Tosatto SC (September 2008). "Evolutionary and ... PDBe-KB provides an overview of all the structure information available in the PDB for Human Glutathione Peroxidase 8 (GPX8) ... structural insights into the multifaceted glutathione peroxidase (Gpx) superfamily". Antioxid. Redox Signal. 10 (9): 1501-14. ...
An ultrastructural and peroxidase-cytochemical study". Lab Invest. 43 (2): 172-81. PMID 7401631. De Vos R, De Wolf-Peeters C, ...
Eosinophil peroxidase deficiency is a condition that affects certain white blood cells called eosinophils but causes no health ... In eosinophil peroxidase deficiency, eosinophils have little or no eosinophil peroxidase. A lack of this protein does not seem ... Eosinophil peroxidase deficiency is estimated to occur in 8.6 in 1,000 Yemenite Jews, in 3 in 1,000 North-African Jews, and in ... Mutations in the EPX gene cause eosinophil peroxidase deficiency. The EPX gene provides instructions for making the eosinophil ...
They identify glutathione peroxidase activity as a robust suppressor of mutant Htt toxicity and validate these protective ... Two of these suppressors encode glutathione peroxidases (GPxs), which are conserved antioxidant enzymes that catalyze the ... Lei, X.G., Cheng, W.H. & McClung, J.P. Metabolic regulation and function of glutathione peroxidase-1. Annu. Rev. Nutr. 27, 41- ... Cellular glutathione peroxidase is the mediator of body selenium to protect against paraquat lethality in transgenic mice. J. ...
Crystal structure of a novel human peroxidase enzyme at 2.0 A resolution.. Choi, H.J., Kang, S.W., Yang, C.H., Rhee, S.G., Ryu ... A set of novel peroxidases, named peroxiredoxins (Prx), regulate the intracellular concentration of H2O2 by reducing it in the ... A set of novel peroxidases, named peroxiredoxins (Prx), regulate the intracellular concentration of H2O2 by reducing it in the ... The positively charged environment surrounding Cys 47 accounts for the peroxidase activity of the enzyme, which contains no ...
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.
... peroxidase effect), and (3) ascorbate (inhibition). Such errors may be diminished by dilution 1:40, or w … ... In the determination of unbound bilirubin by rate of oxidation with peroxidase, errors may be caused by (1) phenol, ... Kinetics of bilirubin oxidation with peroxidase, as applied to studies of bilirubin-albumin binding Scand J Clin Lab Invest. ... In the determination of unbound bilirubin by rate of oxidation with peroxidase, errors may be caused by (1) phenol, ...
More about Describe The Effect Of Ph On Enzyme Catalase And Peroxidase. *. Horseradish Peroxidase Lab Report. 1090 Words , 5 ... One class of enzymes are known as peroxidase. Peroxidase catalyze the oxidation of a particular substrate by hydrogen peroxide ... Horseradish Peroxidase Lab Report. 1090 Words , 5 Pages. ABSTRACT To catalyze a reaction, an enzyme will grab on (bind) to one ... Enzyme Turnip Peroxidase Lab Report. 1117 Words , 5 Pages. The effect of pH on the speed of enzyme interaction with substrate ...
Har du spørsmål om noe vedrørende publikasjonen, kan du kontakte Nofimas bibliotekleder.. Kjetil ...
Peroxidase-immobilized porous silica particles for in situ formation of peroxidase-free hydrogels with attenuated immune ... "Horseradish Peroxidase" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... This graph shows the total number of publications written about "Horseradish Peroxidase" by people in Harvard Catalyst Profiles ... Detecting Horseradish Peroxidase-Labeled Cells. Cold Spring Harb Protoc. 2019 04 01; 2019(4). ...
Assessment of peroxidase-like activity of metal and metal oxide nanoparticles ... Nanotechnologies - Assessment of peroxidase-like activity of metal and metal oxide nanoparticles. ...
Browse our Glutathione Peroxidase 3/GPX3 ELISA Kits all backed by our Guarantee+. ... Glutathione Peroxidase 3/GPX3 ELISA Kits available through Novus Biologicals. ...
Glutathione peroxidase 1. Kind. protein. Organism. Humans. Protein. Name. UniProt ID. Glutathione peroxidase 1. P07203. Details ...
The as-prepared nanocrystalline cobalt selenide was found to possess peroxidase-like activity that could catalyze the reaction ... Keywords: enzyme mimics, cobalt selenide, peroxidase-like activity, uric acid, human serum ... of peroxidase substrates in the presence of H2O2. A spectrophotometric method for uric acid (UA) determination was developed ... Peroxidase-like activity of nanocrystalline cobalt selenide and its application for uric acid detection Quan-Quan Zhuang,1 Zhi- ...
head squash, abdomen squash, based on immunocytochemical streptavidin-biotin-peroxidase complex (‎ISBPC)‎ assay. Determination ... antibody DSSC7 for detecting dengue infection in Aedes aegypti based on immunocytochemical streptavidin biotin peroxidase ... antibody DSSC7 for detecting dengue infection in Aedes aegypti based on immunocytochemical streptavidin biotin peroxidase ...
Apoperoxidase pH 2.1 elutes much later about the same elution volume as neutral enzyme than peroxidase pH 2.1 indicating a ... A molecular weight of about 80,000 for the peroxidase-hemin aggregate has been determined by membrane osmometry. ... On gel filtration of peroxidase immediately after acidification, hemin and the protein elute together. ... Analytical sedimentation velocity and sucrose density gradient sedimentation of peroxidase immediately after acidification ...
Glutathione Peroxidase 4 Is Associated with Neuromelanin in Substantia Nigra and Dystrophic Axons in Putamen of Parkinsons ...
Structure, function and industrial applications of plant laccases and peroxidases. *Fry, Stephen (Principal Investigator) ...
Find symptoms and other information about Eosinophil peroxidase deficiency. ... Eosinophil peroxidase deficiency. Other Names: EPXD; Eosinophil peroxidase deficiency, partial; Peroxidase and phospholipid ... Eosinophil peroxidase deficiency is a rare abnormality of eosinophil granulocytes characterized by decreased or absent ... deficiency in eosinophils; Presentey anomalyEPXD; Eosinophil peroxidase deficiency, partial; Peroxidase and phospholipid ...
Rabbit polyclonal antibody to Glutathione Peroxidase 2 (glutathione peroxidase 2 (gastrointestinal)) ...
Seminars and Events at the Research Institute of Molecular Pathology (IMP) and Vienna Biocenter (VBC).
Chapter 3: Understanding the reactivity and interactions of peroxidases with substrates. In Heme Peroxidases. 4 ed. Royal ... most notably in cytochrome c peroxidase and manganese peroxidase. For many years the location of the substrate binding ... most notably in cytochrome c peroxidase and manganese peroxidase. For many years the location of the substrate binding ... most notably in cytochrome c peroxidase and manganese peroxidase. For many years the location of the substrate binding ...
The other tested antioxidant enzymes such as catalase, glutathione peroxidase and glutathione S transferase also showed marked ... Diabetes, fucoidan, antioxidant enzymes, free radicals, superoxide dismutase, catalase, glutathione peroxidase, glutathione S- ... Peroxidase (GPx), Glutathione S-Transferase (GST) and increased activity of lipid hydroperoxidase [19]. Therefore, it has been ... Biochemical role as a component of glutathione peroxidase. Science 1973;179(4073):588-90. ...
Included in this group are myeloperoxidase, eosinophil peroxidase, lactoperoxidase, and thyroid peroxidase. These enzymes are ... The mammalian heme peroxidases (MHPs) are a medically important group of enzymes. ... The phylogeny of the mammalian heme peroxidases and the evolution of their diverse functions ... The phylogeny of the mammalian heme peroxidases and the evolution of their diverse functions. BMC Evolutionary Biology, 8 . pp ...
Peroxidase Assay Kit. Antibodies, Recombinant proteins, ELISA kits, RNAi, cDNA clones, Antibody Array, Luminex kits. Reagents ... Products for research , Metabolism assay kits , Assay kits - Oxidative stress , Assay kit - Peroxidase > Peroxidase Assay Kit ...
Extensive applications of peroxidase (POX) have raised the global market demand at a considerable rate during the forecast ... About 80% of the discovered POXs belong to heme-peroxidases, which are categorized in two large families of peroxidase- ... The produced peroxidase showed 4 times higher affinity for phenol, as compared with that of HRP, as well as an exceptionally ... In pursuit of this trend, a novel heme peroxidase was purified to homogeneity (MW of 40 kD) from the callus culture of Ocimum ...
The orientation suggests an electron-transfer pathway between the haem groups of cytochrome C and cytochrome C peroxidase that ... The bimolecular ET complex between cytochrome C and cytochrome C peroxidase produced by co-crystallization of cytochrome C with ... the Zn derivative of cytochrome C peroxidase. ...
2 Fe(II)-[cytochrome c] + 2 H(+) + H2O2 <=> 2 Fe(III)-[cytochrome c] + 2 ...
Class I peroxidase / Heme-binding peroxidase Ccp1-like / Peroxidase; domain 2 / Peroxidase, domain 2 / Peroxidase; domain 1 - # ... Class I peroxidase / Heme-binding peroxidase Ccp1-like / Peroxidase; domain 2 / Peroxidase, domain 2 / Peroxidase; domain 1 - # ... Peroxidase; domain 1 / Plant heme peroxidase family profile. / Peroxidase / Haem peroxidase / Peroxidases proximal heme-ligand ... Peroxidase, active site / Peroxidases active site signature. / Peroxidase; domain 1 / Plant heme peroxidase family profile. ... ...
New Roles for an Old Selenoenzyme: Evidence from Glutathione Peroxidase-1 Null and Overexpressing Mice.. Journal Title:. ... glutathione peroxidase. antioxidants. literature reviews. reactive oxygen species. oxidative stress. selenium. nutritional ...
Peroxidase, Brassica oleracea gongylodes, Immunoglobulins, Periodate treatment, Conjugation Abstract. Peroxidase tagged ... The glycoprotein nature of peroxidases can be exploited for conjugation to proteins of interest. Peroxidase extracted from the ... Conjugation of Peroxidase from Brassica oleracea gongylodes for Use as a Label- Prospect of a Novel Enzyme Tag for Immunoassay ... Shetty, P., DSouza, A., & CP, G. (2017). Conjugation of Peroxidase from Brassica oleracea gongylodes for Use as a Label- ...
  • The enzyme used was horseradish peroxidase which helps catalyze hydrogen peroxide. (
  • Horseradish Peroxidase" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (
  • This graph shows the total number of publications written about "Horseradish Peroxidase" by people in Harvard Catalyst Profiles by year, and whether "Horseradish Peroxidase" was a major or minor topic of these publication. (
  • Below are the most recent publications written about "Horseradish Peroxidase" by people in Profiles. (
  • Detecting Horseradish Peroxidase-Labeled Cells. (
  • The use of chromogenic, fluorescent, and chemiluminescent electron donors with horseradish peroxidase (HRP) has proven highly useful for detecting H 2 O 2 and tracking reactions that produce H 2 O 2 . (
  • Role of Hemin in the Acid Aggregation of Horseradish Peroxidase. (
  • Expulsion of hemin from horseradish peroxidase at pH 2.1 is accompained by aggregation of the protein. (
  • For instance, a techno-economic analysis study has shown that the transformed Nicotiana benthamiana can be used for production of horseradish peroxidase (HRP) in a biofarm platform with the proven plant yield of 240 mg HRP kg − 1 biomass (Walwyn et al. (
  • Horseradish peroxidase (EC, in presence of hydrogen peroxide catalyses the oxidation of [Os(bpy)2Cl(pyCOOH)]Cl. (
  • Here we describe an effective strategy to obtain crystal structures for high-valency redox intermediates and present a three-dimensional movie of the X-ray-driven catalytic reduction of a bound dioxygen species in horseradish peroxidase (HRP). (
  • First description of the x-ray structures of classical horseradish peroxidase in all five oxidation states, including the catalytically relevant Fe (IV) intermediates and first catalytic movie for dioxygen reduction, cited 157 times. (
  • The kit contains: chemiluminescent substrate, positive control (horseradish peroxidase), assay buffer, and an optimized 'mix and read' assay protocol that is compatible with HTS liquid handling instruments. (
  • Horseradish peroxidase (HRP) is a popular enzyme used extensively in molecular biology to detect immune complexes and biological targets such as proteins, carbohydrates, and nucleic acids. (
  • Each molecule of a Poly-HRP conjugate contains a multitude of HRP (polymerized horseradish peroxidase), supplying more enzymatic activity per binding event and consequently a dramatic signal amplification in conventional assay applications. (
  • Horseradish Peroxidase (HRP) plays important roles in many biotechnological fields, including diagnostics, biosensors and biocatalysis. (
  • Conjugated to horseradish peroxidase (HRP) via reductive amination. (
  • Then a biotinylated detection antibody specific for Rat AChE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. (
  • One class of enzymes are known as peroxidase. (
  • However, as with other enzymes, stability is a major problem that restricts the application of peroxidase. (
  • 11 , 12 Nanoscale peroxidase mimics possess several advantages over naturally occurring peroxidase enzymes, including almost-unchanged catalytic activity over a wide range of pH and temperatures, low cost, tunable catalytic activities, as well as ease of storage and treatment. (
  • The heme peroxidase enzymes catalyze the HO 2 -dependent oxidation of a wide variety of substrates. (
  • The other tested antioxidant enzymes such as catalase, glutathione peroxidase and glutathione S transferase also showed marked regain in a dose-dependent manner upon administration of fucoidan. (
  • The mammalian heme peroxidases (MHPs) are a medically important group of enzymes. (
  • In nature, efficient lignin degraders, white-rot fungi, secrete enzymes collectively termed "ligninases" in which the most important and active enzyme is lignin peroxidase. (
  • A book The Peroxidase Multigene Family of Enzymes: Biochemical off of this file helps the edition of invalid 1920s, which spend next states and connections. (
  • The book The Peroxidase Multigene Family of Enzymes: Biochemical Basis and will learn illustrated to your Kindle exposure. (
  • 1818042, ' book The Peroxidase Multigene Family of Enzymes: Biochemical Basis and Clinical Applications 2000 ': ' A usual island with this phone time exactly takes. (
  • book The Peroxidase Multigene Family of Enzymes: Biochemical Basis and Clinical Applications ': ' management backgrounds can drape all politicians of the Page. (
  • In the mild book The Peroxidase Multigene Family of Enzymes:, the designs for ordinary energy description wish taken. (
  • Peroxidase (EC is one of the key enzymes controlling plant growth, differentiation and development. (
  • Sodium dodecyl sulfate−polyacrylamide gel electrophoresis revealed that the purified enzymes were homogeneous and peroxidase of leaves have two isoenzyme with molecular weight of approximately 56 and 33 kDa, but peroxidase of fruits has a isoenzyme with molecular weight of approximately 55 kDa. (
  • These effects are counteracted by various oxidative defense enzymes and anti-oxidants such as glutathione peroxidase isoforms GPx1 and GPx4, glutathione reductase (GR), and cellular glutathion. (
  • Self-assemblies of magnetic nanoparticles (MNPs) and peroxidase enzymes: mesoporous structures and nanoscale magnetic field effects (nano-MFEs) for enhanced activity BioNanoCatalysts (BNCs). (
  • BNCs are self-assembled complexes of magnetic nanoparticles and free-radical producing enzymes, including peroxidases and other oxido-reductases that were shown to have higher activities than their free-enzyme counterparts (up to 30 folds). (
  • Although, peroxidase-like activities were observed with magnetite nanoparticles themselves, our results demonstrates that it is the complex of the enzymes with the magnetic nanoparticles that is responsible for the enhanced peroxidase activity of the enzyme, acting more specifically on the overall dynamic of the free-radical cofactors. (
  • Unlike selenomethionine, which is incorporated into proteins in place of methionine, SeMC is not incorporated into any proteins so it is fully available for the synthesis of selenium-containing enzymes, such as glutathione peroxidase. (
  • The positively charged environment surrounding Cys 47 accounts for the peroxidase activity of the enzyme, which contains no redox cofactors. (
  • Throughout this experiment, we conducted a study on peroxidase, which is an enzyme. (
  • Peroxidase is the most reliable and accessible enzyme to use as it can be easily prepared and tested (Ramesh Kumar 1). (
  • In this experiment we used Turnip Peroxidase as our enzyme. (
  • In addition, peroxidase-labeled immunoglobulin is a common probe in immunohistochemistry and in enzyme amplification immunoassay systems. (
  • Apoperoxidase pH 2.1 elutes much later about the same elution volume as neutral enzyme than peroxidase pH 2.1 indicating a hemin requirement for the aggregation. (
  • Peroxidase tagged proteins are being used successfully as immune-histological probes for the demonstration of tissue antigens, and in enzyme amplified immunoassay systems for the quantitative determination of soluble and insoluble antigens. (
  • One type of sensor measures the concentration of hydrogen peroxide using a thermostable peroxidase enzyme that is immobilized in a redox hydrogel to form a sensing layer on a working electrode. (
  • Although lignin peroxidase is claimed as a key enzyme in enzyme-catalyzed lignin degradation, in vitro enzymatic degradation of lignin was not easily observed in lab-scale experiments. (
  • Irreversible interaction between phenolic compound and lignin peroxidase was hypothesized when active enzyme could not be recovered after the reaction with degradation product (guaiacol) of lignin phenolic dimer. (
  • Rat IgG F(ab')2 Fragment Peroxidase Conjugated is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. (
  • Eosinophil peroxidase is a haloperoxidase enzyme that in humans is encoded by the EPX gene . (
  • The enzyme is a heterodimeric 71-77 kD peroxidase consisting of a heavier glycosylated chain and a lighter nonglycosylated chain. (
  • We are therefore reporting a novel enzyme-magnetic nanoparticles system to carry enhanced peroxidase activities for the production of aromatic free-radicals and subsequent peroxidation reactions including polymerization and depolymerization of aromatics. (
  • The Thyroid Profile consists of a battery of several tests for the measurement of thyroid function, including Total and Free Thyroxine, Total and Free Triiodothyronine, Thyroglobulin, Thyroglobulin Antibodies, Thyroid Peroxidase Antibodies, and Thyroid Stimulating Hormone. (
  • Identification of novel genetic Loci associated with thyroid peroxidase antibodies and clinical thyroid disease. (
  • Individuals with positive thyroid peroxidase antibodies (TPOAbs) have an increased risk of autoimmune hypothyroidism (Hashimoto's thyroiditis ), as well as autoimmune hyperthyroidism ( Graves' disease ). (
  • The pres- ence of thyroid antibodies (anti-thyroid peroxidase and anti-thyroglobulin) was also assessed. (
  • domain 1 / Plant heme peroxidase family profile. (
  • Eosinophil peroxidase deficiency is a condition that affects certain white blood cells called eosinophils but causes no health problems in affected individuals. (
  • One of these proteins is called eosinophil peroxidase. (
  • In eosinophil peroxidase deficiency, eosinophils have little or no eosinophil peroxidase. (
  • Because eosinophil peroxidase deficiency does not cause any health problems, this condition is often diagnosed when blood tests are done for other reasons or when a family member has been diagnosed with the condition. (
  • Approximately 100 individuals with eosinophil peroxidase deficiency have been described in the scientific literature. (
  • Eosinophil peroxidase deficiency is estimated to occur in 8.6 in 1,000 Yemenite Jews, in 3 in 1,000 North-African Jews, and in 1 in 1,000 Iraqi Jews. (
  • and in Luxembourg, eosinophil peroxidase deficiency is thought to occur in 1 in 100,000 people. (
  • Mutations in the EPX gene cause eosinophil peroxidase deficiency. (
  • The EPX gene provides instructions for making the eosinophil peroxidase protein. (
  • During an immune response, activated eosinophils release eosinophil peroxidase at the site of injury. (
  • EPX gene mutations reduce or prevent eosinophil peroxidase production or result in a protein that is unstable and nonfunctional. (
  • As a result, eosinophils have severely reduced amounts of eosinophil peroxidase or none at all. (
  • Other proteins within affected eosinophils are normal, and while the cells lacking eosinophil peroxidase are smaller and may have structural changes, the loss of eosinophil peroxidase does not appear to impair the function of eosinophils. (
  • Kutter D, Mueller-Hagedorn S, Forges T, Glaesener R. A case of eosinophil peroxidase deficiency. (
  • Romano M, Patriarca P, Melo C, Baralle FE, Dri P. Hereditary eosinophil peroxidase deficiency: immunochemical and spectroscopic studies and evidence for a compound heterozygosity of the defect. (
  • Eosinophil peroxidase deficiency is a rare abnormality of eosinophil granulocytes characterized by decreased or absent peroxidase activity and decreased volume of the granule matrix (summary by Romano et al. (
  • The objective of the study was to compare nasal, pharyngeal, and sputum eosinophil peroxidase (EPX) levels with induced sputum eosinophil percentage in 10 adults with poorly controlled asthma and 10 normal controls. (
  • to get instant updates about 'Eosinophil Peroxidase' on your MyPage . (
  • Eosinophil peroxidase is a haloperoxidase that preferentially uses bromide over chloride for this purpose, generating hypobromite ( hypobromous acid ). (
  • Eosinophil peroxidase is also partly responsible for tissue remodeling. (
  • The oxidizing compounds produced by eosinophil peroxidase have been implicated in the inflammatory pathology of several disease states, including asthma. (
  • In most cases the substrate is a small organic molecule, but there are famous exceptions, most notably in cytochrome c peroxidase and manganese peroxidase. (
  • The bimolecular ET complex between cytochrome C and cytochrome C peroxidase produced by co-crystallization of cytochrome C with the Zn derivative of cytochrome C peroxidase. (
  • The orientation suggests an electron-transfer pathway between the haem groups of cytochrome C and cytochrome C peroxidase that includes tryptophan. (
  • A method is presented for determining the relative contributions of each of the four O 2 -consuming reactions, i.e. the cytochrome pathway, the alternative pathway, the "residual component" and the peroxidase-mediated O 2 uptake. (
  • abstract = "Peroxidase, isolated from B16 mouse melanoma, converted tyrosine to dopachrome in the presence of either dopa or dihydroxyfumarate co-factor. (
  • In the study of lignin peroxidase isozyme H8 from white-rot fungi Phanerochaete chrysosporium (LiPH8), W251 site was revealed to make the covalent coupling with one moiety of monolignolic radical (guaiacol radical) by LC-MS/MS analysis. (
  • Lignin peroxidases from white-rot fungi, lignin peroxidase isozyme H8 (LiPH8) from Phanerochaete chrysosporium harbors exposed catalytic W171 site which was demonstrated to play a vital role in the oxidation of high-redox potential substrates such as veratryl alcohol (VA) or non-phenolic lignin derivatives. (
  • It has recently been discovered that lignin peroxidase isozyme H2 (LiPH2) has the ability to oxidize Mn2+ (Khindaria et al. (
  • This peroxidase was stimulated by 5 mM SHAM, whereas ascorbic acid, catalase, catechol, gentisic acid, low concentrations potassium cyanide (3.5 μM), sodium azide, sodium sulfide, superoxide dismutase and high concentrations SHAM (25 mM) inhibited this reaction. (
  • IL-1B is elevated in coal miners but not superoxide dismutase, hydrogen peroxide or glutathione peroxidase. (
  • Peroxidase catalyze the oxidation of a particular substrate by hydrogen peroxide. (
  • Hydrogen peroxide-activated Coprinus Cinereus peroxidase (CIP) can initiate polymerization of tyrosine-containing peptides via initial formation of an intermediate tyrosyl radical, which for the first time has been identified by spin trap electron spin resonance spectroscopy as located on carbon 1 in the aromatic ring, and subsequent formation of either dityrosine or isodityrosine bonds through a net elimination of two hydrogen atoms between peptides. (
  • Alveolar macrophages were incubated with nothing, coal, silica or zymosan particles for 12 hours and the fluids analyzed for the release of (SOD), for the release of hydrogen peroxidase (H2O2), and glutathione peroxidase (GSH). (
  • On gel filtration of peroxidase immediately after acidification, hemin and the protein elute together. (
  • Specific activity of 15.3 and 31.1 U mg−1 protein for peroxidase of leaves and fruits were earned, respectively. (
  • These include the genes coding for sucrose synthase, sedoheptulose-1,7-bisphosphatase, a bZIP protein (EmBP-1), a peroxidase and an abscisic acid-induced protein (#7). (
  • IMSEAR at SEARO: Solubilisation & properties of mitochondrial peroxidase from mouse gastric mucosa. (
  • By using the information in this website, you accept our She decided known on book The Peroxidase Multigene Family of of Damian, I think. (
  • A series of site-directed mutants, E35Q, E39Q, and E35Q-D179N, in the gene encoding manganese peroxidase isozyme 1 (mnp1) from Phanerochaete chrysosporium, was created by overlap extension, using the polymerase chain reaction. (
  • The as-prepared nanocrystalline cobalt selenide was found to possess peroxidase-like activity that could catalyze the reaction of peroxidase substrates in the presence of H 2 O 2 . (
  • The stimulation at low concentrations resulted from a SHAM-stimulated peroxidase activity, whereas 25 mM SHAM completely inhibited both the peroxidase-mediated O 2 uptake and the activity of the alternative pathway. (
  • Activity of peroxidase in leaves and friuts in presence of guaiacol and H2O2 were optimum after incubation at 40 and 45°C, respectively. (
  • The SensoLyte® Luminescent Peroxidase Assay Kit provides highly sensitive chemiluminescent substrate to quantify peroxidase activity in solutions, in cell extracts, in live cells. (
  • 2,3,7,8-Tetrachlorodibenzo- p -dioxin as an antiestrogen: Effect on rat uterine peroxidase activity. (
  • 3. Block endogenous peroxidase activity by incubating for 10 min in 3% H2O2. (
  • Peroxidase activity was significantly decreased as storage period increased. (
  • Foliar application with boron had the highest peroxidase activity in both seasons. (
  • Effect of leucaena aqueous extract on the development, mitotic index and peroxidase activity in maize seedlings. (
  • For many years the location of the substrate binding interactions were not known, but more recent structural information for a number of peroxidases with a wide range of different substrates has meant that a more detailed picture of substrate binding to peroxidases is now available. (
  • Kwon, H, Moody, PCE & Raven, EL 2016, Chapter 3: Understanding the reactivity and interactions of peroxidases with substrates . (
  • However, hydrazines are regarded as a poor peroxidase substrates because inactivation of the peroxidase occurs during oxidation of these compounds. (
  • The glycoprotein nature of peroxidases can be exploited for conjugation to proteins of interest. (
  • In the forwarded hypothesis for the reaction mechanism upon peroxidase-initiated cross-linking of tyrosine-containing peptides and proteins, it is suggested that the polymerization takes place through a radical chain reaction. (
  • head squash, abdomen squash, based on immunocytochemical streptavidin-biotin-peroxidase complex (‎ISBPC)‎ assay. (
  • Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Rat IgG, anti-Rat IgG F(ab')2 and anti-Rat Serum. (
  • Rat IgG F(ab')2 Fragment Peroxidase Conjugated reagents are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods. (
  • Anti-human IgM (µ chain guanidine hydrochloride, in the diluent of specific) goat serum and peroxidase- antibody in the ELISA. (
  • This chapter examines the nature of these substrate binding interactions across the family of peroxidases, gathering evidence from published structures of peroxidase-substrate complexes. (
  • Lignin is the natural substrate of ligninolytic peroxidase, even though it is bulky and very recalcitrant toward degradation. (
  • As one of the most commonly used biomedical testing tools, peroxidase can catalyze redox reactions between electron donors and H 2 O 2 . (
  • Peroxidases catalyze oxidation-reduction reactions and play an important role in protecting cell from oxidative injury. (
  • The purpose of this study is to investigate the effects of varying the concentration of peroxidase on rate of reaction, as well as, the varying temperature and pH levels. (
  • We demonstrate here that this fourth O 2 -consuming reaction is mediated by a peroxidase. (
  • This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region followed by Peroxidase conjugation. (
  • Nakagawa T, Ikemoto T, Takeuchi T, Tanaka K, Tanigawa N, Yamamoto D, Shimizu A. Eosinophilic peroxidase deficiency: Identification of a point mutation (D648N) and prediction of structural changes. (
  • Diagnosis is established by the presence of characteristic eosinophilic peroxidase-positive giant granules in leukocytes. (
  • Glutathione peroxidase 3 gene polymorphisms and the risk of sudden sensorineural hearing loss. (
  • The C718T polymorphism in the 3'-untranslated region of glutathione peroxidase-4 gene is a predictor of cerebral stroke in patients with essential hypertension. (
  • The mutant manganese peroxidases (MnPs) were purified and characterized. (
  • Haem peroxidase / Peroxidases proximal heme-ligand signature. (
  • NaSCN did not affect the reactions between anti-human IgM and patients' IgM, and between dengue viral antigens and detecting antibody, peroxidase-conjugated flavivirus-specific monoclonal antibody D1-4G2 IgG. (
  • These features make nanoscale peroxidase mimics suitable candidates for potential applications in the fields of biomedicine and environmental chemistry. (
  • In the determination of unbound bilirubin by rate of oxidation with peroxidase, errors may be caused by (1) phenol, propylparaben, and phenothiazines (free radical acceleration), (2) haemoglobin (peroxidase effect), and (3) ascorbate (inhibition). (
  • As variáveis analisadas foram umidade ponderal, teor de açúcares solúveis, atividade respiratória, atividade das enzimas superóxido dismutase (SOD), catalase (CAT), ascorbato peroxidase (APX), guaiacol peroxidase (POD) e polifenoloxidase (PPO), teor de peróxido de hidrogênio (H2O2) e peroxidação lipídica. (
  • NaN3 and kojic acid were potent inhibitors of peroxidase and ZnSO4 showed that has an activatory effect on peroxidase in Leaves and fruits fo blueberry. (