Peroxidases. Medical search

An enzyme isolated from horseradish which is able to act as an antigen. It is frequently used as a histochemical tracer for light and electron microscopy. Its antigenicity has permitted its use as a combined antigen and marker in experimental immunology.

An enzyme catalyzing the oxidation of 2 moles of glutathione in the presence of hydrogen peroxide to yield oxidized glutathione and water. EC 1.11.1.9.

A hemeprotein from leukocytes. Deficiency of this enzyme leads to a hereditary disorder coupled with disseminated moniliasis. It catalyzes the conversion of a donor and peroxide to an oxidized donor and water. EC 1.11.1.7.

A hemeprotein which catalyzes the oxidation of ferrocytochrome c to ferricytochrome c in the presence of hydrogen peroxide. EC 1.11.1.5.

Peroxidases that utilize ASCORBIC ACID as an electron donor to reduce HYDROGEN PEROXIDE to WATER. The reaction results in the production of monodehydroascorbic acid and DEHYDROASCORBIC ACID.

A 66-kDa peroxidase found in EOSINOPHIL granules. Eosinophil peroxidase is a cationic protein with a pI of 10.8 and is comprised of a heavy chain subunit and a light chain subunit. It possesses cytotoxic activity towards BACTERIA and other organisms, which is attributed to its peroxidase activity.

A hemeprotein that catalyzes the oxidation of the iodide radical to iodine with the subsequent iodination of many organic compounds, particularly proteins. EC 1.11.1.8.

A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials.

An agent thought to have disinfectant properties and used as an expectorant. (From Martindale, The Extra Pharmacopoeia, 30th ed, p747)

An oxidoreductase that catalyzes the conversion of HYDROGEN PEROXIDE to water and oxygen. It is present in many animal cells. A deficiency of this enzyme results in ACATALASIA.

An element with the atomic symbol Se, atomic number 34, and atomic weight 78.96. It is an essential micronutrient for mammals and other animals but is toxic in large amounts. Selenium protects intracellular structures against oxidative damage. It is an essential component of GLUTATHIONE PEROXIDASE.

An enzyme derived from cow's milk. It catalyzes the radioiodination of tyrosine and its derivatives and of peptides containing tyrosine.

A family of ubiquitously-expressed peroxidases that play a role in the reduction of a broad spectrum of PEROXIDES like HYDROGEN PEROXIDE; LIPID PEROXIDES and peroxinitrite. They are found in a wide range of organisms, such as BACTERIA; PLANTS; and MAMMALS. The enzyme requires the presence of a thiol-containing intermediate such as THIOREDOXIN as a reducing cofactor.

The most abundant natural aromatic organic polymer found in all vascular plants. Lignin together with cellulose and hemicellulose are the major cell wall components of the fibers of all wood and grass species. Lignin is composed of coniferyl, p-coumaryl, and sinapyl alcohols in varying ratios in different plant species. (From Merck Index, 11th ed)

A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).

An oxidoreductase that catalyzes the reaction between superoxide anions and hydrogen to yield molecular oxygen and hydrogen peroxide. The enzyme protects the cell against dangerous levels of superoxide. EC 1.15.1.1.

An enzyme that catalyzes the chlorination of a range of organic molecules, forming stable carbon-chloride bonds. EC 1.11.1.10.

A phylum of fungi that produce their sexual spores (basidiospores) on the outside of the basidium. It includes forms commonly known as mushrooms, boletes, puffballs, earthstars, stinkhorns, bird's-nest fungi, jelly fungi, bracket or shelf fungi, and rust and smut fungi.

Alcohols derived from the aryl radical (C6H5CH2-) and defined by C6H5CHOH. The concept includes derivatives with any substituents on the benzene ring.

Naturally occurring or synthetic substances that inhibit or retard the oxidation of a substance to which it is added. They counteract the harmful and damaging effects of oxidation in animal tissues.

Catalyzes the oxidation of GLUTATHIONE to GLUTATHIONE DISULFIDE in the presence of NADP+. Deficiency in the enzyme is associated with HEMOLYTIC ANEMIA. Formerly listed as EC 1.6.4.2.

A disturbance in the prooxidant-antioxidant balance in favor of the former, leading to potential damage. Indicators of oxidative stress include damaged DNA bases, protein oxidation products, and lipid peroxidation products (Sies, Oxidative Stress, 1991, pxv-xvi).

The color-furnishing portion of hemoglobin. It is found free in tissues and as the prosthetic group in many hemeproteins.

A group of compounds that contain a bivalent O-O group, i.e., the oxygen atoms are univalent. They can either be inorganic or organic in nature. Such compounds release atomic (nascent) oxygen readily. Thus they are strong oxidizing agents and fire hazards when in contact with combustible materials, especially under high-temperature conditions. The chief industrial uses of peroxides are as oxidizing agents, bleaching agents, and initiators of polymerization. (From Hawley's Condensed Chemical Dictionary, 11th ed)

A tripeptide with many roles in cells. It conjugates to drugs to make them more soluble for excretion, is a cofactor for some enzymes, is involved in protein disulfide bond rearrangement and reduces peroxides.

Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.

Peroxidase catalyzed oxidation of lipids using hydrogen peroxide as an electron acceptor.

The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.

The rate dynamics in chemical or physical systems.

A genus of fungi in the family Corticiaceae, order Stereales, that degrades lignin. The white-rot fungus Phanerochaete chrysosporium is a frequently used species in research.

Very toxic industrial chemicals. They are absorbed through the skin, causing lethal blood, bladder, liver, and kidney damage and are potent, broad-spectrum carcinogens in most species.

A genus of black-spored basidiomycetous fungi of the family Coprinaceae, order Agaricales; some species are edible.

Inorganic binary compounds of iodine or the I- ion.

Selenoproteins are proteins that specifically incorporate SELENOCYSTEINE into their amino acid chain. Most selenoproteins are enzymes with the selenocysteine residues being responsible for their catalytic functions.

Peroxides produced in the presence of a free radical by the oxidation of unsaturated fatty acids in the cell in the presence of molecular oxygen. The formation of lipid peroxides results in the destruction of the original lipid leading to the loss of integrity of the membranes. They therefore cause a variety of toxic effects in vivo and their formation is considered a pathological process in biological systems. Their formation can be inhibited by antioxidants, such as vitamin E, structural separation or low oxygen tension.

The lectin wheatgerm agglutinin conjugated to the enzyme HORSERADISH PEROXIDASE. It is widely used for tracing neural pathways.

A genus of basidiomycetous fungi, family POLYPORACEAE, order POLYPORALES, that grows on logs or tree stumps in shelflike layers. The species P. ostreatus, the oyster mushroom, is a choice edible species and is the most frequently encountered member of the genus in eastern North America. (Alexopoulos et al., Introductory Mycology, 4th ed, p531)

A selenium compound with the molecular formula H2SO3. It used as a source of SELENIUM, especially for patients that develop selenium deficiency following prolonged PARENTERAL NUTRITION.

Highly reactive molecules with an unsatisfied electron valence pair. Free radicals are produced in both normal and pathological processes. They are proven or suspected agents of tissue damage in a wide variety of circumstances including radiation, damage from environment chemicals, and aging. Natural and pharmacological prevention of free radical damage is being actively investigated.

The dialdehyde of malonic acid.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

A peroxiredoxin that is a cytosolic bifunctional enzyme. It functions as a peroxiredoxin via a single redox-active cysteine and also contains a Ca2+-independent acidic phospholipase A2 activity.

The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.

A highly vascularized endocrine gland consisting of two lobes joined by a thin band of tissue with one lobe on each side of the TRACHEA. It secretes THYROID HORMONES from the follicular cells and CALCITONIN from the parafollicular cells thereby regulating METABOLISM and CALCIUM level in blood, respectively.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)

A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.

A THIOREDOXIN-dependent hydroperoxidase that is localized in the mitochondrial matrix. The enzyme plays a crucial role in protecting mitochondrial components from elevated levels of HYDROGEN PEROXIDE.

An extracellular selenoprotein that contains most of the SELENIUM in PLASMA. Selenoprotein P functions as an antioxidant and appears to transport selenium from the LIVER to peripheral tissues.

Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.

Proteins that contain an iron-porphyrin, or heme, prosthetic group resembling that of hemoglobin. (From Lehninger, Principles of Biochemistry, 1982, p480)

A naturally occurring amino acid in both eukaryotic and prokaryotic organisms. It is found in tRNAs and in the catalytic site of some enzymes. The genes for glutathione peroxidase and formate dehydrogenase contain the TGA codon, which codes for this amino acid.

A family of bracket fungi, order POLYPORALES, living in decaying plant matter and timber.

Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.

A six carbon compound related to glucose. It is found naturally in citrus fruits and many vegetables. Ascorbic acid is an essential nutrient in human diets, and necessary to maintain connective tissue and bone. Its biologically active form, vitamin C, functions as a reducing agent and coenzyme in several metabolic pathways. Vitamin C is considered an antioxidant.

An order of fungi in the phylum BASIDIOMYCOTA having macroscopic basidiocarps. The members are characterized by their saprophytic activities as decomposers, particularly in the degradation of CELLULOSE and LIGNIN. A large number of species in the order have been used medicinally. (From Alexopoulos, Introductory Mycology, 4th ed, pp504-68)

Molecules or ions formed by the incomplete one-electron reduction of oxygen. These reactive oxygen intermediates include SINGLET OXYGEN; SUPEROXIDES; PEROXIDES; HYDROXYL RADICAL; and HYPOCHLOROUS ACID. They contribute to the microbicidal activity of PHAGOCYTES, regulation of signal transduction and gene expression, and the oxidative damage to NUCLEIC ACIDS; PROTEINS; and LIPIDS.

Low-molecular-weight end products, probably malondialdehyde, that are formed during the decomposition of lipid peroxidation products. These compounds react with thiobarbituric acid to form a fluorescent red adduct.

The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)

An enzyme of the oxidoreductase class that catalyzes the conversion of beta-D-glucose and oxygen to D-glucono-1,5-lactone and peroxide. It is a flavoprotein, highly specific for beta-D-glucose. The enzyme is produced by Penicillium notatum and other fungi and has antibacterial activity in the presence of glucose and oxygen. It is used to estimate glucose concentration in blood or urine samples through the formation of colored dyes by the hydrogen peroxide produced in the reaction. (From Enzyme Nomenclature, 1992) EC 1.1.3.4.

Inorganic salts of HYDROGEN CYANIDE containing the -CN radical. The concept also includes isocyanides. It is distinguished from NITRILES, which denotes organic compounds containing the -CN radical.

Granular leukocytes with a nucleus that usually has two lobes connected by a slender thread of chromatin, and cytoplasm containing coarse, round granules that are uniform in size and stainable by eosin.

Enzymes which are immobilized on or in a variety of water-soluble or water-insoluble matrices with little or no loss of their catalytic activity. Since they can be reused continuously, immobilized enzymes have found wide application in the industrial, medical and research fields.

A trace element with atomic symbol Mn, atomic number 25, and atomic weight 54.94. It is concentrated in cell mitochondria, mostly in the pituitary gland, liver, pancreas, kidney, and bone, influences the synthesis of mucopolysaccharides, stimulates hepatic synthesis of cholesterol and fatty acids, and is a cofactor in many enzymes, including arginase and alkaline phosphatase in the liver. (From AMA Drug Evaluations Annual 1992, p2035)

A transferase that catalyzes the addition of aliphatic, aromatic, or heterocyclic FREE RADICALS as well as EPOXIDES and arene oxides to GLUTATHIONE. Addition takes place at the SULFUR. It also catalyzes the reduction of polyol nitrate by glutathione to polyol and nitrite.

An extensive order of basidiomycetous fungi whose fruiting bodies are commonly called mushrooms.

A direct-acting oxidative stress-inducing agent used to examine the effects of oxidant stress on Ca(2+)-dependent signal transduction in vascular endothelial cells. It is also used as a catalyst in polymerization reactions and to introduce peroxy groups into organic molecules.

A plant genus of the family BRASSICACEAE known for the root used in hot SPICES. It is also the source of HORSERADISH PEROXIDASE which is widely used in laboratories.

Organic derivatives of thiocyanic acid which contain the general formula R-SCN.

Diagnostic aid in pancreas function determination.

An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.

Inorganic salts of the hypothetical acid ferrocyanic acid (H4Fe(CN)6).

A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.

Electron-accepting molecules in chemical reactions in which electrons are transferred from one molecule to another (OXIDATION-REDUCTION).

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Benzene derivatives that include one or more hydroxyl groups attached to the ring structure.

A copper-containing oxidoreductase enzyme that catalyzes the oxidation of 4-benzenediol to 4-benzosemiquinone. It also has activity towards a variety of O-quinols and P-quinols. It primarily found in FUNGI and is involved in LIGNIN degradation, pigment biosynthesis and detoxification of lignin-derived products.

Analysis of the intensity of Raman scattering of monochromatic light as a function of frequency of the scattered light.

The engulfing of liquids by cells by a process of invagination and closure of the cell membrane to form fluid-filled vacuoles.

Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.

A GLUTATHIONE dimer formed by a disulfide bond between the cysteine sulfhydryl side chains during the course of being oxidized.

A group of compounds that are derivatives of methoxybenzene and contain the general formula R-C7H7O.

Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

The disodium salt of selenious acid. It is used therapeutically to supply the trace element selenium and is prepared by the reaction of SELENIUM DIOXIDE with SODIUM HYDROXIDE.

A non-selective post-emergence, translocated herbicide. According to the Seventh Annual Report on Carcinogens (PB95-109781, 1994) this substance may reasonably be anticipated to be a carcinogen. (From Merck Index, 12th ed) It is an irreversible inhibitor of CATALASE, and thus impairs activity of peroxisomes.

Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.

Inflammatory disease of the THYROID GLAND due to autoimmune responses leading to lymphocytic infiltration of the gland. It is characterized by the presence of circulating thyroid antigen-specific T-CELLS and thyroid AUTOANTIBODIES. The clinical signs can range from HYPOTHYROIDISM to THYROTOXICOSIS depending on the type of autoimmune thyroiditis.

Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.

Salts of hydrobromic acid, HBr, with the bromine atom in the 1- oxidation state. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

A generic descriptor for all TOCOPHEROLS and TOCOTRIENOLS that exhibit ALPHA-TOCOPHEROL activity. By virtue of the phenolic hydrogen on the 2H-1-benzopyran-6-ol nucleus, these compounds exhibit varying degree of antioxidant activity, depending on the site and number of methyl groups and the type of ISOPRENOIDS.

Hydrogen-donating proteins that participates in a variety of biochemical reactions including ribonucleotide reduction and reduction of PEROXIREDOXINS. Thioredoxin is oxidized from a dithiol to a disulfide when acting as a reducing cofactor. The disulfide form is then reduced by NADPH in a reaction catalyzed by THIOREDOXIN REDUCTASE.

A genus of fungi in the family Coriolaceae.

Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.

Organic compounds which contain selenium as an integral part of the molecule.

Proteins prepared by recombinant DNA technology.

A FLAVOPROTEIN enzyme that catalyzes the oxidation of THIOREDOXINS to thioredoxin disulfide in the presence of NADP+. It was formerly listed as EC 1.6.4.5

A group of physiologically active prostaglandin endoperoxides. They are precursors in the biosynthesis of prostaglandins and thromboxanes. Most frequently encountered member of this group is the prostaglandin G2.

The measurement of the amplitude of the components of a complex waveform throughout the frequency range of the waveform. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)

The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.

Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.

Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.

Compounds containing the -SH radical.

Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.

Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)

Diazo derivatives of aniline, used as a reagent for sugars, ketones, and aldehydes. (Dorland, 28th ed)

A plant genus in the family LILIACEAE (sometimes placed in Asparagaceae) that contains ECDYSTEROIDS and is an ingredient of Siotone. The shoots are used as a vegetable and the roots are used in FOLK MEDICINE.

Elements of limited time intervals, contributing to particular results or situations.

Hydroxycinnamic acid and its derivatives. Act as activators of the indoleacetic acid oxidizing system, thereby producing a decrease in the endogenous level of bound indoleacetic acid in plants.

The facilitation of biochemical reactions with the aid of naturally occurring catalysts such as ENZYMES.

An antiseptic and disinfectant aromatic alcohol.

The parts of a macromolecule that directly participate in its specific combination with another molecule.

Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.

A cytochrome oxidase inhibitor which is a nitridizing agent and an inhibitor of terminal oxidation. (From Merck Index, 12th ed)

Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.

The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.

The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.

A product from the iodination of MONOIODOTYROSINE. In the biosynthesis of thyroid hormones, diiodotyrosine residues are coupled with other monoiodotyrosine or diiodotyrosine residues to form T4 or T3 thyroid hormones (THYROXINE and TRIIODOTHYRONINE).

The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.

Stimulation of renin release from rabbit renal cortex by arachidonic acid and prostaglandin endoperoxides. (1/2940)

The mechanism by which renal prostaglandins stimulate renin secretion in vivo is unknown. In this in vitro study we measured the effects of activation of the prostaglandin (PG) system on renin release from slices of rabbit renal cortex. The PG precursor arachidonic acid (C20:4), a natural PG endoperoxide (PGG2), two stable synthetic PG endoperoxide analogues (EPA I and II), PGE2, PGF2alpha, and two different PG synthesis inhibitors [indomethacin and 5,8,11,14-eicosatetraynoic acid (ETA)] were used to evaluate the possibility of a direct action of the cortical PG system on renin secretion. Renin release increased significantly with time after addition of C20:4, PGG2, EPA I, and EPA II to the incubation medium. Stimulation of renin release was se-related for C20:4 in concentrations of 0.6 to 4.5 X 10(-6) M, for EPA I in concentrations of 0.7 to 2.8 X 10(-6) M, and for EPA II in concentrations of 1.4 to 14.0 X 10(-6) M. Indomethacin (10(-4) M) and ETA (10(-4) M) significantly decreased basal renin release as well as the renin release stimulated by C20:4 and EPA I. PGE2(10(-12) to 10(-6) M) had no effect on renin release, whereas PGF2alpha (10(-12) to 10(-6) M) decreased renin release in a dose-dependent manner. These data raise the possibility of a direct action of the renal cortical PG system on renin secretion. The results further indicate that stimulation of renin release by C20:4 may depend more specifically on the action of PG endoperoxides than on the primary prostaglandins. (+info)

Isolation and purification of rat mammary tumor peroxidase. (2/2940)

7,12-Dimethylbenz(a)anthracene-induced rat mammary tumors often contain high levels of the enzyme perioxidase, a putative marker of estrogen dependence. This enzyme can be effectively extracted with 0.5 M CaCl2, giving rise to a soluble peroxidase with a molecular weight of about 50,000 as determined by gel filtration. This is the same size as the estrogen-induced peroxidase of rat uterus but smaller than other mammalian peroxidases. Further purification of the rat mammary tumor peroxidase by concanavalin A-Sepharose chromatography and hydrophobic interaction chromatography on phenyl Sepharose provides a 640-fold purification of the enzyme. (+info)

Cloning of the peroxiredoxin gene family in rats and characterization of the fourth member. (3/2940)

Peroxiredoxin (PRx) exhibits thioredoxin-dependent peroxidase activity and constitutes a family of proteins. Four members of genes from rat tissues were isolated by PCR using degenerated primers based on the sequences which encode a pair of highly conserved Cys-containing domains, and were then cloned to full-length cDNAs. These included two genes which have previously been isolated in rats, PRx I and PRx II, and two rat homologues of PRx III and PRx IV. We showed, for the first time, the simultaneous expression of all four genes in various rat tissues by Northern blotting. Since a discrepancy exists regarding cellular distribution, we further characterized PRx IV by expressing it in COS-1 cells. This clearly demonstrates that PRx IV is a secretory form and functions within the extracellular space. (+info)

Purification and characterization of a novel peroxidase from Geotrichum candidum dec 1 involved in decolorization of dyes. (4/2940)

A peroxidase (DyP) involved in the decolorization of dyes and produced by the fungus strain Geotrichum candidum Dec 1 was purified. DyP, a glycoprotein, is glycosylated with N-acetylglucosamine and mannose (17%) and has a molecular mass of 60 kDa and an isoelectric point (pI) of 3.8. The absorption spectrum of DyP exhibited a Soret band at 406 nm corresponding to a hemoprotein, and its Na2S2O4-reduced form revealed a peak at 556 nm that indicates the presence of a protoheme as its prosthetic group. Nine of the 21 types of dyes that were decolorized by Dec 1 cells were decolorized by DyP; in particular, anthraquinone dyes were highly decolorized. DyP also oxidized 2,6-dimethoxyphenol and guaiacol but not veratryl alcohol. The optimal temperature for DyP activity was 30 degrees C, and DyP activity was stable even after incubation at 50 degrees C for 11 h. (+info)

Phospholipid hydroperoxide cysteine peroxidase activity of human serum albumin. (5/2940)

Human serum albumin (HSA) reduced the phospholipid hydroperoxide, 1-palmitoyl-2-(13-hydroperoxy-cis-9, trans-11-octadecadienoyl)-l-3-phosphatidylcholine (PLPC-OOH) to the corresponding hydroxy-derivative with a high apparent affinity (Km=9. 23+/-0.95 microM). Removal of bound lipid during purification increased this activity. At physiological concentration, HSA reduced the phospholipid hydroperoxide in the absence of a cofactor. However, in the presence of a cofactor (reductant), the rate of the reaction was increased. All of the major aminothiols in plasma could act as reductants, the best being the most abundant, cysteine (Km=600+/-80 microM). For every nanomole of PLPC-OOH reduced by HSA, 1.26 nmol of cystine was formed, indicating a reaction stoichiometry of 1 mol PLPC-OOH to 2 mol cysteine. We used chemical modification to determine which amino acid residues on HSA were responsible for the activity. Oxidation of thiol group(s) by N-ethylmaleimide led to a reduction in the rate of activity, whereas reduction of thiols by either dithiothreitol or the angiotensin-converting enzyme inhibitor, captopril, increased the activity. Both N-ethylmaleimide-modified HSA and dithiothreitol-treated HSA exhibited increased apparent affinities for PLPC-OOH. For a range of preparations of albumin with different modifications, the activity on PLPC-OOH was dependent on the amount of free thiol groups on the albumin (correlation coefficient=0.91). Patients with lowered albumin concentrations after septic shock showed lowered total plasma thiol concentrations and decreased phospholipid hydroperoxide cysteine peroxidase (PHCPx) activities. These results therefore show for the first time that HSA exhibits PHCPx activity, and that the majority of the activity depends on the presence of reduced thiol group(s) on the albumin. (+info)

Use of site-directed mutagenesis to probe the structure, function and isoniazid activation of the catalase/peroxidase, KatG, from Mycobacterium tuberculosis. (6/2940)

A series of mutants bearing single amino acid substitutions often encountered in the catalase/peroxidase, KatG, from isoniazid-resistant isolates of Mycobacterium tuberculosis has been produced by site-directed mutagenesis. The resultant enzymes were overexpressed, purified and characterized. Replacing Cys-20 by Ser abolished disulphide-bridge formation, but did not affect either dimerization of the enzyme or catalysis. The substitution of Thr-275, which is probably involved in electron transfer from the haem, by proline resulted in a highly unstable enzyme with insignificant enzyme activities. The most commonly occurring substitution in drug-resistant clinical isolates is the replacement of Ser-315 by Thr; this lowered catalase and peroxidase activities by 50% and caused a significant decrease in the KatG-mediated inhibition of the activity of the NADH-dependent enoyl-[acyl-carrier protein] reductase, InhA, in vitro. The ability of this enzyme to produce free radicals from isoniazid was severely impaired, as judged by its loss of NitroBlue Tetrazolium reduction activity. Replacement of Leu-587 by Pro resulted in marked instability of KatG, indicating that the C-terminal domain is also important for structural and functional integrity. (+info)

Direct interaction of lignin and lignin peroxidase from Phanerochaete chrysosporium. (7/2940)

Binding properties of lignin peroxidase (LiP) from the basidiomycete Phanerochaete chrysosporium against a synthetic lignin (dehydrogenated polymerizate, DHP) were studied with a resonant mirror biosensor. Among several ligninolytic enzymes, only LiP specifically binds to DHP. Kinetic analysis revealed that the binding was reversible, and that the dissociation equilibrium constant was 330 microM. The LiP-DHP interaction was controlled by the ionization group with a pKa of 5.3, strongly suggesting that a specific amino acid residue plays a role in lignin binding. A one-electron transfer from DHP to oxidized intermediates LiP compounds I and II (LiPI and LiPII) was characterized by using a stopped-flow technique, showing that binding interactions of DHP with LiPI and LiPII led to saturation kinetics. The dissociation equilibrium constants for LiPI-DHP and LiPII-DHP interactions were calculated to be 350 and 250 microM, and the first-order rate constants for electron transfer from DHP to LiPI and to LiPII were calculated to be 46 and 16 s-1, respectively. These kinetic and spectral studies strongly suggest that LiP is capable of oxidizing lignin directly at the protein surface by a long-range electron transfer process. A close look at the crystal structure suggested that LiP possesses His-239 as a possible lignin-binding site on the surface, which is linked to Asp-238. This Asp residue is hydrogen-bonded to the proximal His-176. This His-Asp...proximal-His motif would be a possible electron transfer route to oxidize polymeric lignin. (+info)

Eosinophil peroxidase increases membrane permeability in mammalian urinary bladder epithelium. (8/2940)

Eosinophil peroxidase (EPO), a cationic protein found in eosinophils, has been reported to be cytotoxic independent of its peroxidase activity. This study investigated with electrophysiological methods whether EPO is toxic to mammalian urinary bladder epithelium. Results indicate that EPO, when added to the mucosal solution, increases apical membrane conductance of urinary bladder epithelium only when the apical membrane potential is cell interior negative. The EPO-induced conductance was concentration dependent, with a maximum conductance of 411 microseconds/cm2 and a Michaelis-Menten constant of 113 nM. The EPO-induced conductance was nonselective for K+ and Cl-. The conductance was partially reversed using voltage but not by removal of EPO from the bulk solution. Mucosal Ca2+ reversed the EPO-induced conductance by a mechanism involving reversible block of the conductance. Prolonged exposure (up to 1 h) to EPO was toxic to the urinary bladder epithelium, as indicated by an irreversible increase in transepithelial conductance. These results suggest that EPO is indeed toxic to urinary bladder epithelium via a mechanism that involves an increase in membrane permeability. (+info)

... catalyzed by the presence of a peroxidase (such as horseradish peroxidase). The hydrogen peroxide is itself produced by an ... "Peroxidases". In Ashok Pandey; Colin Webb; Carlos Ricardo Soccol; Christian Larroche (eds.). Enzyme technology. Springer. p. ...

https://en.wikipedia.org/wiki/Trinder_glucose_activity_test

Banerjee, R. K.; Bose, A. K.; Chakraborty, T. K.; De, S. K.; Datta, A. G. (1985). "Peroxidase-catalysed iodotyrosine formation ... Banerjee, R. K.; Datta, A. G. (1986). "Salivary peroxidases". Mol Cell Biochem. 70 (1): 21-29. doi:10.1007/bf00233801. PMID ... lysozyme and peroxidase. It has not been shown that human licking of wounds disinfects them, but licking is likely to help ...

https://en.wikipedia.org/wiki/Saliva

Dunford BH (1999). Heme peroxidases. John Wiley. ISBN 0-471-24244-6. Dawson JH (April 1988). "Probing structure-function ... as exemplified by peroxidases, catalases, reductases, etc.) are ubiquitous in cellular systems. While several moiety and ... relations in heme-containing oxygenases and peroxidases". Science. 240 (4851): 433-439. Bibcode:1988Sci...240..433D. doi: ...

https://en.wikipedia.org/wiki/Murburn_concept

Enzyme kinetics Glutathione peroxidase Peroxidase Superoxide dismutase GRCh38: Ensembl release 89: ENSG00000121691 - Ensembl, ... "PeroxiBase - The peroxidase database". Swiss Institute of Bioinformatics. Archived from the original on 2008-10-13. Retrieved ... Maehly AC, Chance B (1954). "The assay of catalases and peroxidases". Methods of Biochemical Analysis. Methods of Biochemical ... contains catalases and peroxidases. To activate the noxious spray, the beetle mixes the contents of the two compartments, ...

https://en.wikipedia.org/wiki/Catalase

... produces chitinase and peroxidase. Wang, W; Mai-Gisondi, G; Stogios, PJ; Kaur, A; Xu, X; Cui, H; ... Iqbal, M.; Mercer, D. K.; Miller, P. G. G.; McCarthy, A. J. (1 June 1994). "Thermostable extracellular peroxidases from ...

https://en.wikipedia.org/wiki/Streptomyces_thermoviolaceus

... thyroid peroxidase activity; and hormone release. TSHR is involved in regulating seasonal reproduction in vertebrates. Graves' ...

https://en.wikipedia.org/wiki/Thyrotropin_receptor

The research on these lignin-degrading enzymes produced by Bjerkandera adusta, such as versatile peroxidase, has also shown in ... ISBN 978-0-88192-935-5. Singh, R.; Eltis, L.D. (2015). "The multihued palette of dye-decolorizing peroxidases". Archives of ... "Lignin-degrading peroxidases in Polyporales: an evolutionary survey based on 10 sequenced genomes". Mycologia. 105 (6): 1428- ... "Functional Characterization of Melanin Decolorizing Extracellular Peroxidase of Bjerkandera adusta". Journal of Fungi. 7 (9): ...

https://en.wikipedia.org/wiki/Bjerkandera_adusta

There are at least four different glutathione peroxidase isozymes in animals. Glutathione peroxidase 1 is the most abundant and ... Glutathione peroxidase is an enzyme containing four selenium-cofactors that catalyzes the breakdown of hydrogen peroxide and ... Surprisingly, glutathione peroxidase 1 is dispensable, as mice lacking this enzyme have normal lifespans, but they are ... In addition to its direct antioxidant effects, ascorbic acid is also a substrate for the redox enzyme ascorbate peroxidase, a ...

https://en.wikipedia.org/wiki/Antioxidant

Peroxidases are also involved for some species. The body of the prey is decomposed by a cocktail of hydrolytic enzymes which ...

https://en.wikipedia.org/wiki/Carnivorous_plant

Bacteria do not express any of the plant-type peroxidases (lignin peroxidase, Mn peroxidase, or versatile peroxidases), but ... Some ligninolytic enzymes include heme peroxidases such as lignin peroxidases, manganese peroxidases, versatile peroxidases, ... Lignin peroxidases oxidize non-phenolic lignin, whereas manganese peroxidases only oxidize the phenolic structures. Dye- ... Both peroxidase and laccase enzymes are present in the plant cell walls, and it is not known whether one or both of these ...

https://en.wikipedia.org/wiki/Lignin

It was first limited to class III peroxidases (plant peroxidases) and was then expanded to include all possible haem and non- ... The majority of haem and non-haem peroxidase sequences can now be found in the PeroxiBase. The database is hosted by the Swiss ... Peroxidase sequences come from other general public databases (NCBI, TIGR, UniProt KnowledgeBase: all the databases used are ... haem peroxidase protein sequences. Many researchers and bioinformaticians from the University of Geneva joined their efforts to ...

https://en.wikipedia.org/wiki/PeroxiBase

PRSS7 Eosinophil peroxidase deficiency; 261500; EPX Epidermodysplasia verruciformis; 226400; TMC6 Epidermodysplasia ...

https://en.wikipedia.org/wiki/List_of_OMIM_disorder_codes

... eosinophil peroxidase (EPO), thyroid peroxidase (TPO), and prostaglandin H synthase (PGHS). A heme cofactor is bound near the ... Lactoperoxidase is a peroxidase enzyme secreted from mammary, salivary and other mucosal glands including the lungs, bronchii ... Peroxidase-generated hypoiodous acid (HOI), hypoiodite and hypothiocyanite all destroy the herpes simplex virus and human ... Lactoperoxidase is a member of the heme peroxidase family of enzymes. In humans, lactoperoxidase is encoded by the LPO gene. ...

https://en.wikipedia.org/wiki/Lactoperoxidase

These systems include peroxidases, catalases, and superoxide dismutases. A complementary metalloprotein to those that react ...

https://en.wikipedia.org/wiki/Bioinorganic_chemistry

ISBN 978-1-947988-83-5. India portal Biology portal Medicine portal Lactoferrin Haem peroxidase Lactoperoxidase Please see ... "Structural basis of activation of mammalian heme peroxidases". Progress in Biophysics and Molecular Biology. 133: 49-55. doi: ...

https://en.wikipedia.org/wiki/Sujata_Sharma

Anti-thyroid peroxidase (anti-TPO) antibodies are specific for the autoantigen TPO, a 105kDa glycoprotein that catalyses iodine ... McLachlan SM, Rapoport B (2000). "Autoimmune response to the thyroid in humans: thyroid peroxidase--the common autoantigenic ... The most clinically relevant anti-thyroid autoantibodies are anti-thyroid peroxidase antibodies (anti-TPO antibodies, TPOAb), ... Taurog A (May 1999). "Molecular evolution of thyroid peroxidase". Biochimie. 81 (5): 557-62. doi:10.1016/S0300-9084(99)80110-2 ...

https://en.wikipedia.org/wiki/Antithyroid_autoantibodies

Glutathione peroxidase activity of inorganic selenium and seleno-DL-cysteine. Experientia 31: 769-770, 1975 Beutler E, et al. ... Electrophoretic polymorphism of glutathione peroxidase. Ann Hum Genet 38: 163-169, 1974 Beutler E, et al. ...

https://en.wikipedia.org/wiki/Ernest_Beutler

The enzyme is thyroid peroxidase. The small amount of T3 could be important because different tissues have different ...

https://en.wikipedia.org/wiki/Triiodothyronine

... l is one important characteristic of animal peroxidases; plant peroxidases incorporate heme B. Lactoperoxidase and ... In peroxidase reactions, the porphyrin molecule also serves as an electron source, being able to delocalize radical electrons ... Heme m contains the two ester bonds at the heme 1- and 5-methyl groups also present in heme l of other mammalian peroxidases, ... In general, diatomic gases only bind to the reduced heme, as ferrous Fe(II) while most peroxidases cycle between Fe(III) and Fe ...

https://en.wikipedia.org/wiki/Heme

Some bacteria have NADH-dependent peroxidases specific for H2O2. The main nonenzymatic antioxidants in E. coli are GSH and ...

https://en.wikipedia.org/wiki/Oxidation_response

White WE, Pruitt KM, Mansson-Rahemtulla B (February 1983). "Peroxidase-Thiocyanate-Peroxide Antibacterial System Does Not ... Hypothiocyanite is formed by peroxidase catalysis of hydrogen peroxide and thiocyanate: H2O2 + SCN− → OSCN− + H2O ... "Active site structure and catalytic mechanisms of human peroxidases". Arch. Biochem. Biophys. 445 (2): 199-213. doi:10.1016/j. ... respiratory syncytial virus and echovirus type 11 by peroxidase-generated hypothiocyanite". Antiviral Res. 26 (2): 161-71. doi: ...

https://en.wikipedia.org/wiki/Hypothiocyanite

Here, trypanothione-dependent enzymes such as tryparedoxin peroxidase (TryP) reduce peroxides using electrons donated either ... Trypanothione-dependent enzymes include reductases, peroxidases, glyoxalases and transferases. Trypanothione-disulfide ...

https://en.wikipedia.org/wiki/Trypanothione

"New and classic families of secreted fungal heme peroxidases". Appl Microbiol Biotechnol. 87 (3): 871-897. doi:10.1007/s00253- ...

https://en.wikipedia.org/wiki/Hypochlorite

"Proximity-based protein thiol oxidation by H2O2-scavenging peroxidases". The Journal of Biological Chemistry. 284 (46): 31532- ...

https://en.wikipedia.org/wiki/RoGFP

Norisoprenoids, such as C13-norisoprenoids found in grape (Vitis vinifera) or wine, can be produced by fungal peroxidases or ... "Generation of Norisoprenoid Flavors from Carotenoids by Fungal Peroxidases". Journal of Agricultural and Food Chemistry. 57 (21 ...

https://en.wikipedia.org/wiki/Wine_chemistry

... can be produced by fungal peroxidases or glycosidases. While terpenes and terpenoids occur widely, their extraction from ... "Generation of Norisoprenoid Flavors from Carotenoids by Fungal Peroxidases". Journal of Agricultural and Food Chemistry. 57 (21 ...

https://en.wikipedia.org/wiki/Terpene

Glutathione peroxidase 3 (GPx-3), also known as plasma glutathione peroxidase (GPx-P) or extracellular glutathione peroxidase ... Lin JC, Kuo WR, Chiang FY, Hsiao PJ, Lee KW, Wu CW, Juo SH (May 2009). "Glutathione peroxidase 3 gene polymorphisms and risk of ... "Entrez Gene: glutathione peroxidase 3 (plasma)". Takahashi K, Avissar N, Whitin J, Cohen H (August 1987). "Purification and ... Saga Y, Ohwada M, Suzuki M, Konno R, Kigawa J, Ueno S, Mano H (December 2008). "Glutathione peroxidase 3 is a candidate ...

https://en.wikipedia.org/wiki/GPX3

Glutathione peroxidase 7 is an enzyme that in humans is encoded by the GPX7 gene. GRCh38: Ensembl release 89: ENSG00000116157 ... PDBe-KB provides an overview of all the structure information available in the PDB for Human Glutathione peroxidase 7 Portal: ... "Entrez Gene: GPX7 glutathione peroxidase 7". Opalenik SR, Ding Q, Mallery SR, Thompson JA (1998). "Glutathione depletion ... 2001). "Human immunodeficiency virus type 1 Tat protein impairs selenoglutathione peroxidase expression and activity by a ...

https://en.wikipedia.org/wiki/GPX7

Glutathione peroxidase 8 (GPx-8) is an enzyme that in humans is encoded by the GPX8 gene. GPx-8 is a member of the glutathione ... "Entrez Gene: glutathione peroxidase 8 (putative)". Toppo S, Vanin S, Bosello V, Tosatto SC (September 2008). "Evolutionary and ... PDBe-KB provides an overview of all the structure information available in the PDB for Human Glutathione Peroxidase 8 (GPX8) ... structural insights into the multifaceted glutathione peroxidase (Gpx) superfamily". Antioxid. Redox Signal. 10 (9): 1501-14. ...

https://en.wikipedia.org/wiki/GPX8

An ultrastructural and peroxidase-cytochemical study". Lab Invest. 43 (2): 172-81. PMID 7401631. De Vos R, De Wolf-Peeters C, ...

https://en.wikipedia.org/wiki/Epithelioid_cell

Eosinophil peroxidase deficiency is a condition that affects certain white blood cells called eosinophils but causes no health ... In eosinophil peroxidase deficiency, eosinophils have little or no eosinophil peroxidase. A lack of this protein does not seem ... Eosinophil peroxidase deficiency is estimated to occur in 8.6 in 1,000 Yemenite Jews, in 3 in 1,000 North-African Jews, and in ... Mutations in the EPX gene cause eosinophil peroxidase deficiency. The EPX gene provides instructions for making the eosinophil ...

https://medlineplus.gov/genetics/condition/eosinophil-peroxidase-deficiency/

They identify glutathione peroxidase activity as a robust suppressor of mutant Htt toxicity and validate these protective ... Two of these suppressors encode glutathione peroxidases (GPxs), which are conserved antioxidant enzymes that catalyze the ... Lei, X.G., Cheng, W.H. & McClung, J.P. Metabolic regulation and function of glutathione peroxidase-1. Annu. Rev. Nutr. 27, 41- ... Cellular glutathione peroxidase is the mediator of body selenium to protect against paraquat lethality in transgenic mice. J. ...

https://www.nature.com/articles/ng.2732?error=cookies_not_supported&code=e6b6236a-58e5-4809-b8d2-d666860ac6f4

Crystal structure of a novel human peroxidase enzyme at 2.0 A resolution.. Choi, H.J., Kang, S.W., Yang, C.H., Rhee, S.G., Ryu ... A set of novel peroxidases, named peroxiredoxins (Prx), regulate the intracellular concentration of H2O2 by reducing it in the ... A set of novel peroxidases, named peroxiredoxins (Prx), regulate the intracellular concentration of H2O2 by reducing it in the ... The positively charged environment surrounding Cys 47 accounts for the peroxidase activity of the enzyme, which contains no ...

https://www.rcsb.org/structure/1prx

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

https://www.sigmaaldrich.com/CA/en/technical-documents/technical-article/protein-biology/immunohistochemistry/colorimetric-alkaline

... peroxidase effect), and (3) ascorbate (inhibition). Such errors may be diminished by dilution 1:40, or w … ... In the determination of unbound bilirubin by rate of oxidation with peroxidase, errors may be caused by (1) phenol, ... Kinetics of bilirubin oxidation with peroxidase, as applied to studies of bilirubin-albumin binding Scand J Clin Lab Invest. ... In the determination of unbound bilirubin by rate of oxidation with peroxidase, errors may be caused by (1) phenol, ...

https://pubmed.ncbi.nlm.nih.gov/523962/

More about Describe The Effect Of Ph On Enzyme Catalase And Peroxidase. *. Horseradish Peroxidase Lab Report. 1090 Words , 5 ... One class of enzymes are known as peroxidase. Peroxidase catalyze the oxidation of a particular substrate by hydrogen peroxide ... Horseradish Peroxidase Lab Report. 1090 Words , 5 Pages. ABSTRACT To catalyze a reaction, an enzyme will grab on (bind) to one ... Enzyme Turnip Peroxidase Lab Report. 1117 Words , 5 Pages. The effect of pH on the speed of enzyme interaction with substrate ...

https://www.ipl.org/essay/Describe-The-Effect-Of-Ph-On-Enzyme-F3CX7RH4NDVV

Har du spørsmål om noe vedrørende publikasjonen, kan du kontakte Nofimas bibliotekleder.. Kjetil ...

https://nofima.no/publikasjon/1179066/

Peroxidase-immobilized porous silica particles for in situ formation of peroxidase-free hydrogels with attenuated immune ... "Horseradish Peroxidase" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... This graph shows the total number of publications written about "Horseradish Peroxidase" by people in Harvard Catalyst Profiles ... Detecting Horseradish Peroxidase-Labeled Cells. Cold Spring Harb Protoc. 2019 04 01; 2019(4). ...

https://connects.catalyst.harvard.edu/Profiles/display/Concept/Horseradish%20Peroxidase

Assessment of peroxidase-like activity of metal and metal oxide nanoparticles ... Nanotechnologies - Assessment of peroxidase-like activity of metal and metal oxide nanoparticles. ...

https://www.iso.org/standard/80771.html

Browse our Glutathione Peroxidase 3/GPX3 ELISA Kits all backed by our Guarantee+. ... Glutathione Peroxidase 3/GPX3 ELISA Kits available through Novus Biologicals. ...

https://www.novusbio.com/elisa-kits/glutathione-peroxidase-3-gpx3?related_pathways=Transport

Glutathione peroxidase 1. Kind. protein. Organism. Humans. Protein. Name. UniProt ID. Glutathione peroxidase 1. P07203. Details ...

https://go.drugbank.com/bio_entities/BE0000816

The as-prepared nanocrystalline cobalt selenide was found to possess peroxidase-like activity that could catalyze the reaction ... Keywords: enzyme mimics, cobalt selenide, peroxidase-like activity, uric acid, human serum ... of peroxidase substrates in the presence of H2O2. A spectrophotometric method for uric acid (UA) determination was developed ... Peroxidase-like activity of nanocrystalline cobalt selenide and its application for uric acid detection Quan-Quan Zhuang,1 Zhi- ...

https://www.dovepress.com/peroxidase-like-activity-of-nanocrystalline-cobalt-selenide-and-its-ap-peer-reviewed-fulltext-article-IJN

head squash, abdomen squash, based on immunocytochemical streptavidin-biotin-peroxidase complex (‎ISBPC)‎ assay. Determination ... antibody DSSC7 for detecting dengue infection in Aedes aegypti based on immunocytochemical streptavidin biotin peroxidase ... antibody DSSC7 for detecting dengue infection in Aedes aegypti based on immunocytochemical streptavidin biotin peroxidase ...

https://extranet.who.int/iris/restricted/handle/10665/170473

Apoperoxidase pH 2.1 elutes much later about the same elution volume as neutral enzyme than peroxidase pH 2.1 indicating a ... A molecular weight of about 80,000 for the peroxidase-hemin aggregate has been determined by membrane osmometry. ... On gel filtration of peroxidase immediately after acidification, hemin and the protein elute together. ... Analytical sedimentation velocity and sucrose density gradient sedimentation of peroxidase immediately after acidification ...

https://apps.dtic.mil/sti/citations/ADA019088

Glutathione Peroxidase 4 Is Associated with Neuromelanin in Substantia Nigra and Dystrophic Axons in Putamen of Parkinsons ...

https://www.sri.com/publication/biosciences-health-pubs/glutathione-peroxidase-4-is-associated-with-neuromelanin-in-substantia-nigra-and-dystrophic-axons-in-putamen-of-parkinsons-brain/

... (RUO) ...

https://www.bdbiosciences.com/en-ch/products/reagents/immunoassay-reagents/elisa/horseradish-peroxidase-hrp-mouse-anti-armenian-and-syrian-hamster-igg-cocktail.554012

Structure, function and industrial applications of plant laccases and peroxidases. *Fry, Stephen (Principal Investigator) ...

https://www.research.ed.ac.uk/en/projects/structure-function-and-industrial-applications-of-plant-laccases-

Find symptoms and other information about Eosinophil peroxidase deficiency. ... Eosinophil peroxidase deficiency. Other Names: EPXD; Eosinophil peroxidase deficiency, partial; Peroxidase and phospholipid ... Eosinophil peroxidase deficiency is a rare abnormality of eosinophil granulocytes characterized by decreased or absent ... deficiency in eosinophils; Presentey anomalyEPXD; Eosinophil peroxidase deficiency, partial; Peroxidase and phospholipid ...

https://rarediseases.info.nih.gov/diseases/12361/eosinophil-peroxidase-deficiency/

Rabbit polyclonal antibody to Glutathione Peroxidase 2 (glutathione peroxidase 2 (gastrointestinal)) ...

https://www.amsbio.com/glutathione-peroxidase-2-gpx2-rabbit-polyclonal-antibody-ta308741

Seminars and Events at the Research Institute of Molecular Pathology (IMP) and Vienna Biocenter (VBC).

https://www.imp.ac.at/calendar/event/the-role-of-glutathione-peroxidase-4-in-cell-fate-decisions/

Chapter 3: Understanding the reactivity and interactions of peroxidases with substrates. In Heme Peroxidases. 4 ed. Royal ... most notably in cytochrome c peroxidase and manganese peroxidase. For many years the location of the substrate binding ... most notably in cytochrome c peroxidase and manganese peroxidase. For many years the location of the substrate binding ... most notably in cytochrome c peroxidase and manganese peroxidase. For many years the location of the substrate binding ...

https://research-information.bris.ac.uk/en/publications/chapter-3-understanding-the-reactivity-and-interactions-of-peroxi

The other tested antioxidant enzymes such as catalase, glutathione peroxidase and glutathione S transferase also showed marked ... Diabetes, fucoidan, antioxidant enzymes, free radicals, superoxide dismutase, catalase, glutathione peroxidase, glutathione S- ... Peroxidase (GPx), Glutathione S-Transferase (GST) and increased activity of lipid hydroperoxidase [19]. Therefore, it has been ... Biochemical role as a component of glutathione peroxidase. Science 1973;179(4073):588-90. ...

https://www.ijpsonline.com/articles/effect-of-fucoidan-on-the-regulation-of-antioxidant-enzymes-in-hepatorenal-tissues-of-streptozotocininduced-diabetic-rats-4359.html?view=mobile

Included in this group are myeloperoxidase, eosinophil peroxidase, lactoperoxidase, and thyroid peroxidase. These enzymes are ... The mammalian heme peroxidases (MHPs) are a medically important group of enzymes. ... The phylogeny of the mammalian heme peroxidases and the evolution of their diverse functions ... The phylogeny of the mammalian heme peroxidases and the evolution of their diverse functions. BMC Evolutionary Biology, 8 . pp ...

https://doras.dcu.ie/17804/

Peroxidase Assay Kit. Antibodies, Recombinant proteins, ELISA kits, RNAi, cDNA clones, Antibody Array, Luminex kits. Reagents ... Products for research , Metabolism assay kits , Assay kits - Oxidative stress , Assay kit - Peroxidase > Peroxidase Assay Kit ...

https://www.clinisciences.com/en/assay-kit-peroxidase-2742/peroxidase-assay-kit-5005445.html

Extensive applications of peroxidase (POX) have raised the global market demand at a considerable rate during the forecast ... About 80% of the discovered POXs belong to heme-peroxidases, which are categorized in two large families of peroxidase- ... The produced peroxidase showed 4 times higher affinity for phenol, as compared with that of HRP, as well as an exceptionally ... In pursuit of this trend, a novel heme peroxidase was purified to homogeneity (MW of 40 kD) from the callus culture of Ocimum ...

https://www.researchsquare.com/article/rs-227761/v1

The orientation suggests an electron-transfer pathway between the haem groups of cytochrome C and cytochrome C peroxidase that ... The bimolecular ET complex between cytochrome C and cytochrome C peroxidase produced by co-crystallization of cytochrome C with ... the Zn derivative of cytochrome C peroxidase. ...

https://www.chemtube3d.com/bc-26-26/

2 Fe(II)-[cytochrome c] + 2 H(+) + H2O2 <=> 2 Fe(III)-[cytochrome c] + 2 ...

https://enzyme.expasy.org/EC/1.11.1.5

Class I peroxidase / Heme-binding peroxidase Ccp1-like / Peroxidase; domain 2 / Peroxidase, domain 2 / Peroxidase; domain 1 - # ... Class I peroxidase / Heme-binding peroxidase Ccp1-like / Peroxidase; domain 2 / Peroxidase, domain 2 / Peroxidase; domain 1 - # ... Peroxidase; domain 1 / Plant heme peroxidase family profile. / Peroxidase / Haem peroxidase / Peroxidases proximal heme-ligand ... Peroxidase, active site / Peroxidases active site signature. / Peroxidase; domain 1 / Plant heme peroxidase family profile. ... ...

https://pdbj.org/emnavi/quick.php?id=pdb-2eup

New Roles for an Old Selenoenzyme: Evidence from Glutathione Peroxidase-1 Null and Overexpressing Mice.. Journal Title:. ... glutathione peroxidase. antioxidants. literature reviews. reactive oxygen species. oxidative stress. selenium. nutritional ...

https://openagricola.nal.usda.gov/catalog/IND43746821

Peroxidase, Brassica oleracea gongylodes, Immunoglobulins, Periodate treatment, Conjugation Abstract. Peroxidase tagged ... The glycoprotein nature of peroxidases can be exploited for conjugation to proteins of interest. Peroxidase extracted from the ... Conjugation of Peroxidase from Brassica oleracea gongylodes for Use as a Label- Prospect of a Novel Enzyme Tag for Immunoassay ... Shetty, P., DSouza, A., & CP, G. (2017). Conjugation of Peroxidase from Brassica oleracea gongylodes for Use as a Label- ...

https://www.nepjol.info/index.php/IJASBT/article/view/17009

The enzyme used was horseradish peroxidase which helps catalyze hydrogen peroxide. (ipl.org)
Horseradish Peroxidase" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
This graph shows the total number of publications written about "Horseradish Peroxidase" by people in Harvard Catalyst Profiles by year, and whether "Horseradish Peroxidase" was a major or minor topic of these publication. (harvard.edu)
Below are the most recent publications written about "Horseradish Peroxidase" by people in Profiles. (harvard.edu)
Detecting Horseradish Peroxidase-Labeled Cells. (harvard.edu)
The use of chromogenic, fluorescent, and chemiluminescent electron donors with horseradish peroxidase (HRP) has proven highly useful for detecting H 2 O 2 and tracking reactions that produce H 2 O 2 . (dovepress.com)
Role of Hemin in the Acid Aggregation of Horseradish Peroxidase. (dtic.mil)
Expulsion of hemin from horseradish peroxidase at pH 2.1 is accompained by aggregation of the protein. (dtic.mil)
For instance, a techno-economic analysis study has shown that the transformed Nicotiana benthamiana can be used for production of horseradish peroxidase (HRP) in a biofarm platform with the proven plant yield of 240 mg HRP kg − 1 biomass (Walwyn et al. (researchsquare.com)
Horseradish peroxidase (EC 1.11.1.7), in presence of hydrogen peroxide catalyses the oxidation of [Os(bpy)2Cl(pyCOOH)]Cl. (conicet.gov.ar)
Here we describe an effective strategy to obtain crystal structures for high-valency redox intermediates and present a three-dimensional movie of the X-ray-driven catalytic reduction of a bound dioxygen species in horseradish peroxidase (HRP). (sussex.ac.uk)
First description of the x-ray structures of classical horseradish peroxidase in all five oxidation states, including the catalytically relevant Fe (IV) intermediates and first catalytic movie for dioxygen reduction, cited 157 times. (sussex.ac.uk)
The kit contains: chemiluminescent substrate, positive control (horseradish peroxidase), assay buffer, and an optimized 'mix and read' assay protocol that is compatible with HTS liquid handling instruments. (mobitec.com)
Horseradish peroxidase (HRP) is a popular enzyme used extensively in molecular biology to detect immune complexes and biological targets such as proteins, carbohydrates, and nucleic acids. (aatbio.com)
Each molecule of a Poly-HRP conjugate contains a multitude of HRP (polymerized horseradish peroxidase), supplying more enzymatic activity per binding event and consequently a dramatic signal amplification in conventional assay applications. (aatbio.com)
Horseradish Peroxidase (HRP) plays important roles in many biotechnological fields, including diagnostics, biosensors and biocatalysis. (biomedcentral.com)
Conjugated to horseradish peroxidase (HRP) via reductive amination. (atsbio.com)
Then a biotinylated detection antibody specific for Rat AChE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. (caslab.com)

One class of enzymes are known as peroxidase. (ipl.org)
However, as with other enzymes, stability is a major problem that restricts the application of peroxidase. (dovepress.com)
11 , 12 Nanoscale peroxidase mimics possess several advantages over naturally occurring peroxidase enzymes, including almost-unchanged catalytic activity over a wide range of pH and temperatures, low cost, tunable catalytic activities, as well as ease of storage and treatment. (dovepress.com)
The heme peroxidase enzymes catalyze the HO 2 -dependent oxidation of a wide variety of substrates. (bris.ac.uk)
The other tested antioxidant enzymes such as catalase, glutathione peroxidase and glutathione S transferase also showed marked regain in a dose-dependent manner upon administration of fucoidan. (ijpsonline.com)
The mammalian heme peroxidases (MHPs) are a medically important group of enzymes. (dcu.ie)
In nature, efficient lignin degraders, white-rot fungi, secrete enzymes collectively termed "ligninases" in which the most important and active enzyme is lignin peroxidase. (biomedcentral.com)
A book The Peroxidase Multigene Family of Enzymes: Biochemical off of this file helps the edition of invalid 1920s, which spend next states and connections. (boltemedical.com)
The book The Peroxidase Multigene Family of Enzymes: Biochemical Basis and will learn illustrated to your Kindle exposure. (boltemedical.com)
1818042, ' book The Peroxidase Multigene Family of Enzymes: Biochemical Basis and Clinical Applications 2000 ': ' A usual island with this phone time exactly takes. (boltemedical.com)
book The Peroxidase Multigene Family of Enzymes: Biochemical Basis and Clinical Applications ': ' management backgrounds can drape all politicians of the Page. (boltemedical.com)
In the mild book The Peroxidase Multigene Family of Enzymes:, the designs for ordinary energy description wish taken. (boltemedical.com)
Peroxidase (EC 1.11.1.7) is one of the key enzymes controlling plant growth, differentiation and development. (bmmj.org)
Sodium dodecyl sulfate−polyacrylamide gel electrophoresis revealed that the purified enzymes were homogeneous and peroxidase of leaves have two isoenzyme with molecular weight of approximately 56 and 33 kDa, but peroxidase of fruits has a isoenzyme with molecular weight of approximately 55 kDa. (bmmj.org)
These effects are counteracted by various oxidative defense enzymes and anti-oxidants such as glutathione peroxidase isoforms GPx1 and GPx4, glutathione reductase (GR), and cellular glutathion. (uv.es)
Self-assemblies of magnetic nanoparticles (MNPs) and peroxidase enzymes: mesoporous structures and nanoscale magnetic field effects (nano-MFEs) for enhanced activity BioNanoCatalysts (BNCs). (techconnect.org)
BNCs are self-assembled complexes of magnetic nanoparticles and free-radical producing enzymes, including peroxidases and other oxido-reductases that were shown to have higher activities than their free-enzyme counterparts (up to 30 folds). (techconnect.org)
Although, peroxidase-like activities were observed with magnetite nanoparticles themselves, our results demonstrates that it is the complex of the enzymes with the magnetic nanoparticles that is responsible for the enhanced peroxidase activity of the enzyme, acting more specifically on the overall dynamic of the free-radical cofactors. (techconnect.org)
Unlike selenomethionine, which is incorporated into proteins in place of methionine, SeMC is not incorporated into any proteins so it is fully available for the synthesis of selenium-containing enzymes, such as glutathione peroxidase. (drvitaminsolutions.com)

The positively charged environment surrounding Cys 47 accounts for the peroxidase activity of the enzyme, which contains no redox cofactors. (rcsb.org)
Throughout this experiment, we conducted a study on peroxidase, which is an enzyme. (ipl.org)
Peroxidase is the most reliable and accessible enzyme to use as it can be easily prepared and tested (Ramesh Kumar 1). (ipl.org)
In this experiment we used Turnip Peroxidase as our enzyme. (ipl.org)
In addition, peroxidase-labeled immunoglobulin is a common probe in immunohistochemistry and in enzyme amplification immunoassay systems. (dovepress.com)
Apoperoxidase pH 2.1 elutes much later about the same elution volume as neutral enzyme than peroxidase pH 2.1 indicating a hemin requirement for the aggregation. (dtic.mil)
Peroxidase tagged proteins are being used successfully as immune-histological probes for the demonstration of tissue antigens, and in enzyme amplified immunoassay systems for the quantitative determination of soluble and insoluble antigens. (nepjol.info)
One type of sensor measures the concentration of hydrogen peroxide using a thermostable peroxidase enzyme that is immobilized in a redox hydrogel to form a sensing layer on a working electrode. (epo.org)
Although lignin peroxidase is claimed as a key enzyme in enzyme-catalyzed lignin degradation, in vitro enzymatic degradation of lignin was not easily observed in lab-scale experiments. (biomedcentral.com)
Irreversible interaction between phenolic compound and lignin peroxidase was hypothesized when active enzyme could not be recovered after the reaction with degradation product (guaiacol) of lignin phenolic dimer. (biomedcentral.com)
Rat IgG F(ab')2 Fragment Peroxidase Conjugated is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. (rockland.com)
Eosinophil peroxidase is a haloperoxidase enzyme that in humans is encoded by the EPX gene . (rediff.com)
The enzyme is a heterodimeric 71-77 kD peroxidase consisting of a heavier glycosylated chain and a lighter nonglycosylated chain. (rediff.com)
We are therefore reporting a novel enzyme-magnetic nanoparticles system to carry enhanced peroxidase activities for the production of aromatic free-radicals and subsequent peroxidation reactions including polymerization and depolymerization of aromatics. (techconnect.org)

The Thyroid Profile consists of a battery of several tests for the measurement of thyroid function, including Total and Free Thyroxine, Total and Free Triiodothyronine, Thyroglobulin, Thyroglobulin Antibodies, Thyroid Peroxidase Antibodies, and Thyroid Stimulating Hormone. (cdc.gov)
Identification of novel genetic Loci associated with thyroid peroxidase antibodies and clinical thyroid disease. (bvsalud.org)
Individuals with positive thyroid peroxidase antibodies (TPOAbs) have an increased risk of autoimmune hypothyroidism (Hashimoto's thyroiditis ), as well as autoimmune hyperthyroidism ( Graves' disease ). (bvsalud.org)
The pres- ence of thyroid antibodies (anti-thyroid peroxidase and anti-thyroglobulin) was also assessed. (who.int)

domain 1 / Plant heme peroxidase family profile. (pdbj.org)

Eosinophil peroxidase deficiency is a condition that affects certain white blood cells called eosinophils but causes no health problems in affected individuals. (medlineplus.gov)
One of these proteins is called eosinophil peroxidase. (medlineplus.gov)
In eosinophil peroxidase deficiency, eosinophils have little or no eosinophil peroxidase. (medlineplus.gov)
Because eosinophil peroxidase deficiency does not cause any health problems, this condition is often diagnosed when blood tests are done for other reasons or when a family member has been diagnosed with the condition. (medlineplus.gov)
Approximately 100 individuals with eosinophil peroxidase deficiency have been described in the scientific literature. (medlineplus.gov)
Eosinophil peroxidase deficiency is estimated to occur in 8.6 in 1,000 Yemenite Jews, in 3 in 1,000 North-African Jews, and in 1 in 1,000 Iraqi Jews. (medlineplus.gov)
and in Luxembourg, eosinophil peroxidase deficiency is thought to occur in 1 in 100,000 people. (medlineplus.gov)
Mutations in the EPX gene cause eosinophil peroxidase deficiency. (medlineplus.gov)
The EPX gene provides instructions for making the eosinophil peroxidase protein. (medlineplus.gov)
During an immune response, activated eosinophils release eosinophil peroxidase at the site of injury. (medlineplus.gov)
EPX gene mutations reduce or prevent eosinophil peroxidase production or result in a protein that is unstable and nonfunctional. (medlineplus.gov)
As a result, eosinophils have severely reduced amounts of eosinophil peroxidase or none at all. (medlineplus.gov)
Other proteins within affected eosinophils are normal, and while the cells lacking eosinophil peroxidase are smaller and may have structural changes, the loss of eosinophil peroxidase does not appear to impair the function of eosinophils. (medlineplus.gov)
Kutter D, Mueller-Hagedorn S, Forges T, Glaesener R. A case of eosinophil peroxidase deficiency. (medlineplus.gov)
Romano M, Patriarca P, Melo C, Baralle FE, Dri P. Hereditary eosinophil peroxidase deficiency: immunochemical and spectroscopic studies and evidence for a compound heterozygosity of the defect. (medlineplus.gov)
Eosinophil peroxidase deficiency is a rare abnormality of eosinophil granulocytes characterized by decreased or absent peroxidase activity and decreased volume of the granule matrix (summary by Romano et al. (nih.gov)
The objective of the study was to compare nasal, pharyngeal, and sputum eosinophil peroxidase (EPX) levels with induced sputum eosinophil percentage in 10 adults with poorly controlled asthma and 10 normal controls. (elsevier.com)
to get instant updates about 'Eosinophil Peroxidase' on your MyPage . (rediff.com)
Eosinophil peroxidase is a haloperoxidase that preferentially uses bromide over chloride for this purpose, generating hypobromite ( hypobromous acid ). (rediff.com)
Eosinophil peroxidase is also partly responsible for tissue remodeling. (rediff.com)
The oxidizing compounds produced by eosinophil peroxidase have been implicated in the inflammatory pathology of several disease states, including asthma. (rediff.com)

In most cases the substrate is a small organic molecule, but there are famous exceptions, most notably in cytochrome c peroxidase and manganese peroxidase. (bris.ac.uk)
The bimolecular ET complex between cytochrome C and cytochrome C peroxidase produced by co-crystallization of cytochrome C with the Zn derivative of cytochrome C peroxidase. (chemtube3d.com)
The orientation suggests an electron-transfer pathway between the haem groups of cytochrome C and cytochrome C peroxidase that includes tryptophan. (chemtube3d.com)
A method is presented for determining the relative contributions of each of the four O 2 -consuming reactions, i.e. the cytochrome pathway, the alternative pathway, the "residual component" and the peroxidase-mediated O 2 uptake. (edu.au)

abstract = "Peroxidase, isolated from B16 mouse melanoma, converted tyrosine to dopachrome in the presence of either dopa or dihydroxyfumarate co-factor. (elsevier.com)

In the study of lignin peroxidase isozyme H8 from white-rot fungi Phanerochaete chrysosporium (LiPH8), W251 site was revealed to make the covalent coupling with one moiety of monolignolic radical (guaiacol radical) by LC-MS/MS analysis. (biomedcentral.com)
Lignin peroxidases from white-rot fungi, lignin peroxidase isozyme H8 (LiPH8) from Phanerochaete chrysosporium harbors exposed catalytic W171 site which was demonstrated to play a vital role in the oxidation of high-redox potential substrates such as veratryl alcohol (VA) or non-phenolic lignin derivatives. (biomedcentral.com)
It has recently been discovered that lignin peroxidase isozyme H2 (LiPH2) has the ability to oxidize Mn2+ (Khindaria et al. (scite.ai)

This peroxidase was stimulated by 5 mM SHAM, whereas ascorbic acid, catalase, catechol, gentisic acid, low concentrations potassium cyanide (3.5 μM), sodium azide, sodium sulfide, superoxide dismutase and high concentrations SHAM (25 mM) inhibited this reaction. (edu.au)
IL-1B is elevated in coal miners but not superoxide dismutase, hydrogen peroxide or glutathione peroxidase. (cdc.gov)

Peroxidase catalyze the oxidation of a particular substrate by hydrogen peroxide. (ipl.org)
Hydrogen peroxide-activated Coprinus Cinereus peroxidase (CIP) can initiate polymerization of tyrosine-containing peptides via initial formation of an intermediate tyrosyl radical, which for the first time has been identified by spin trap electron spin resonance spectroscopy as located on carbon 1 in the aromatic ring, and subsequent formation of either dityrosine or isodityrosine bonds through a net elimination of two hydrogen atoms between peptides. (vtt.fi)
Alveolar macrophages were incubated with nothing, coal, silica or zymosan particles for 12 hours and the fluids analyzed for the release of (SOD), for the release of hydrogen peroxidase (H2O2), and glutathione peroxidase (GSH). (cdc.gov)

On gel filtration of peroxidase immediately after acidification, hemin and the protein elute together. (dtic.mil)
Specific activity of 15.3 and 31.1 U mg−1 protein for peroxidase of leaves and fruits were earned, respectively. (bmmj.org)
These include the genes coding for sucrose synthase, sedoheptulose-1,7-bisphosphatase, a bZIP protein (EmBP-1), a peroxidase and an abscisic acid-induced protein (#7). (usda.gov)

IMSEAR at SEARO: Solubilisation & properties of mitochondrial peroxidase from mouse gastric mucosa. (who.int)

By using the information in this website, you accept our She decided known on book The Peroxidase Multigene Family of of Damian, I think. (boltemedical.com)

A series of site-directed mutants, E35Q, E39Q, and E35Q-D179N, in the gene encoding manganese peroxidase isozyme 1 (mnp1) from Phanerochaete chrysosporium, was created by overlap extension, using the polymerase chain reaction. (scite.ai)

The as-prepared nanocrystalline cobalt selenide was found to possess peroxidase-like activity that could catalyze the reaction of peroxidase substrates in the presence of H 2 O 2 . (dovepress.com)
The stimulation at low concentrations resulted from a SHAM-stimulated peroxidase activity, whereas 25 mM SHAM completely inhibited both the peroxidase-mediated O 2 uptake and the activity of the alternative pathway. (edu.au)
Activity of peroxidase in leaves and friuts in presence of guaiacol and H2O2 were optimum after incubation at 40 and 45°C, respectively. (bmmj.org)
The SensoLyte® Luminescent Peroxidase Assay Kit provides highly sensitive chemiluminescent substrate to quantify peroxidase activity in solutions, in cell extracts, in live cells. (mobitec.com)
2,3,7,8-Tetrachlorodibenzo- p -dioxin as an antiestrogen: Effect on rat uterine peroxidase activity. (cdc.gov)
3. Block endogenous peroxidase activity by incubating for 10 min in 3% H2O2. (bdbiosciences.com)
Peroxidase activity was significantly decreased as storage period increased. (unideb.hu)
Foliar application with boron had the highest peroxidase activity in both seasons. (unideb.hu)
Effect of leucaena aqueous extract on the development, mitotic index and peroxidase activity in maize seedlings. (bvsalud.org)

For many years the location of the substrate binding interactions were not known, but more recent structural information for a number of peroxidases with a wide range of different substrates has meant that a more detailed picture of substrate binding to peroxidases is now available. (bris.ac.uk)
Kwon, H, Moody, PCE & Raven, EL 2016, Chapter 3: Understanding the reactivity and interactions of peroxidases with substrates . (bris.ac.uk)
However, hydrazines are regarded as a poor peroxidase substrates because inactivation of the peroxidase occurs during oxidation of these compounds. (scite.ai)

The glycoprotein nature of peroxidases can be exploited for conjugation to proteins of interest. (nepjol.info)
In the forwarded hypothesis for the reaction mechanism upon peroxidase-initiated cross-linking of tyrosine-containing peptides and proteins, it is suggested that the polymerization takes place through a radical chain reaction. (vtt.fi)

head squash, abdomen squash, based on immunocytochemical streptavidin-biotin-peroxidase complex (‎ISBPC)‎ assay. (who.int)
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Rat IgG, anti-Rat IgG F(ab')2 and anti-Rat Serum. (rockland.com)

Rat IgG F(ab')2 Fragment Peroxidase Conjugated reagents are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods. (rockland.com)
Anti-human IgM (µ chain guanidine hydrochloride, in the diluent of specific) goat serum and peroxidase- antibody in the ELISA. (who.int)

This chapter examines the nature of these substrate binding interactions across the family of peroxidases, gathering evidence from published structures of peroxidase-substrate complexes. (bris.ac.uk)
Lignin is the natural substrate of ligninolytic peroxidase, even though it is bulky and very recalcitrant toward degradation. (biomedcentral.com)

As one of the most commonly used biomedical testing tools, peroxidase can catalyze redox reactions between electron donors and H 2 O 2 . (dovepress.com)
Peroxidases catalyze oxidation-reduction reactions and play an important role in protecting cell from oxidative injury. (mobitec.com)

The purpose of this study is to investigate the effects of varying the concentration of peroxidase on rate of reaction, as well as, the varying temperature and pH levels. (ipl.org)
We demonstrate here that this fourth O 2 -consuming reaction is mediated by a peroxidase. (edu.au)

This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region followed by Peroxidase conjugation. (rockland.com)

Nakagawa T, Ikemoto T, Takeuchi T, Tanaka K, Tanigawa N, Yamamoto D, Shimizu A. Eosinophilic peroxidase deficiency: Identification of a point mutation (D648N) and prediction of structural changes. (medlineplus.gov)
Diagnosis is established by the presence of characteristic eosinophilic peroxidase-positive giant granules in leukocytes. (medscape.com)

Glutathione peroxidase 3 gene polymorphisms and the risk of sudden sensorineural hearing loss. (cdc.gov)
The C718T polymorphism in the 3'-untranslated region of glutathione peroxidase-4 gene is a predictor of cerebral stroke in patients with essential hypertension. (cdc.gov)

The mutant manganese peroxidases (MnPs) were purified and characterized. (scite.ai)

Haem peroxidase / Peroxidases proximal heme-ligand signature. (pdbj.org)

NaSCN did not affect the reactions between anti-human IgM and patients' IgM, and between dengue viral antigens and detecting antibody, peroxidase-conjugated flavivirus-specific monoclonal antibody D1-4G2 IgG. (who.int)

These features make nanoscale peroxidase mimics suitable candidates for potential applications in the fields of biomedicine and environmental chemistry. (dovepress.com)

In the determination of unbound bilirubin by rate of oxidation with peroxidase, errors may be caused by (1) phenol, propylparaben, and phenothiazines (free radical acceleration), (2) haemoglobin (peroxidase effect), and (3) ascorbate (inhibition). (nih.gov)

As variáveis analisadas foram umidade ponderal, teor de açúcares solúveis, atividade respiratória, atividade das enzimas superóxido dismutase (SOD), catalase (CAT), ascorbato peroxidase (APX), guaiacol peroxidase (POD) e polifenoloxidase (PPO), teor de peróxido de hidrogênio (H2O2) e peroxidação lipídica. (scielo.br)

NaN3 and kojic acid were potent inhibitors of peroxidase and ZnSO4 showed that has an activatory effect on peroxidase in Leaves and fruits fo blueberry. (bmmj.org)

Anatomy5

Organisms11

Diseases1

Chemicals and Drugs75

Analytical, Diagnostic and Therapeutic Techniques and Equipment14

Phenomena and Processes15

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Technology, Industry, Agriculture1

Information Science3

Stimulation of renin release from rabbit renal cortex by arachidonic acid and prostaglandin endoperoxides. (1/2940)

Isolation and purification of rat mammary tumor peroxidase. (2/2940)

Cloning of the peroxiredoxin gene family in rats and characterization of the fourth member. (3/2940)

Purification and characterization of a novel peroxidase from Geotrichum candidum dec 1 involved in decolorization of dyes. (4/2940)

Phospholipid hydroperoxide cysteine peroxidase activity of human serum albumin. (5/2940)

Use of site-directed mutagenesis to probe the structure, function and isoniazid activation of the catalase/peroxidase, KatG, from Mycobacterium tuberculosis. (6/2940)

Direct interaction of lignin and lignin peroxidase from Phanerochaete chrysosporium. (7/2940)

Eosinophil peroxidase increases membrane permeability in mammalian urinary bladder epithelium. (8/2940)

Peroxidases

Horseradish Peroxidase

Glutathione Peroxidase

Peroxidase

Cytochrome-c Peroxidase

Ascorbate Peroxidases

Eosinophil Peroxidase

Iodide Peroxidase

Hydrogen Peroxide

Guaiacol

Catalase

Selenium

Lactoperoxidase

Peroxiredoxins

Lignin

Oxidation-Reduction

Superoxide Dismutase

Chloride Peroxidase

Basidiomycota

Benzyl Alcohols

Antioxidants

Glutathione Reductase

Oxidative Stress

Heme

Peroxides

Glutathione

Histocytochemistry

Lipid Peroxidation

Spectrophotometry

Kinetics

Phanerochaete

Benzidines

Coprinus

Iodides

Selenoproteins

Lipid Peroxides

Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate

Pleurotus

Selenious Acid

Free Radicals

Malondialdehyde

Molecular Sequence Data

Peroxiredoxin VI

Catalysis

Thyroid Gland

3,3'-Diaminobenzidine

Amino Acid Sequence

Hydrogen-Ion Concentration

Electron Spin Resonance Spectroscopy

Peroxiredoxin III

Selenoprotein P

Isoenzymes

Hemeproteins

Selenocysteine

Polyporaceae

Microscopy, Electron

Ascorbic Acid

Polyporales

Reactive Oxygen Species

Thiobarbituric Acid Reactive Substances

Oxidoreductases

Glucose Oxidase

Cyanides

Eosinophils

Enzymes, Immobilized

Manganese

Glutathione Transferase

Agaricales

tert-Butylhydroperoxide

Armoracia

Thyroglobulin

Thiocyanates

Selenomethionine

Oxygen

Ferrocyanides

Substrate Specificity

Oxidants

Liver

Phenols

Laccase