Periodic Acid-Schiff Reaction
Alcian Blue
Schiff Bases
Hematoxylin
Histocytochemistry
Mucins
Mucus
Staining and Labeling
Electrophoresis, Polyacrylamide Gel
Amylopectinosis in fetal and neonatal Quarter Horses. (1/161)
Three Quarter Horses, a stillborn filly (horse No. 1), a female fetus aborted at approximately 6 months of gestation (horse No. 2), and a 1-month-old colt that had been weak at birth (horse No. 3), had myopathy characterized histologically by large spherical or ovoid inclusions in skeletal and cardiac myofibers. Smaller inclusions were also found in brain and spinal cord and in some cells of all other tissues examined. These inclusions were basophilic, red-purple after staining with periodic acid-Schiff (both before and after digestion with diastase), and moderately dark blue after staining with toluidine blue. The inclusions did not react when stained with Congo red. Staining with iodine ranged from pale blue to black. Their ultrastructural appearance varied from amorphous to somewhat filamentous. On the basis of staining characteristics and diastase resistance, we concluded that these inclusions contained amylopectin. A distinctly different kind of inclusion material was also present in skeletal muscle and tongue of horse Nos. 1 and 3. These inclusions were crystalline with a sharply defined ultrastructural periodicity. The crystals were eosinophilic and very dark blue when stained with toluidine blue but did not stain with iodine. Crystals sometimes occurred freely within the myofibers but more often were encased by deposits of amylopectin. This combination of histologic and ultrastructural features characterizes a previously unreported storage disease in fetal and neonatal Quarter Horses, with findings similar to those of glycogen storage disease type IV. We speculate that a severe inherited loss of glycogen brancher enzyme activity may be responsible for these findings. The relation of amylopectinosis to the death of the foals is unknown. (+info)Comparative morphology of the cloacal scent gland in snakes (Serpentes: Reptilia). (2/161)
The histology of the cloacal scent gland, or anal gland, was examined from a diverse group of 50 snakes. Extensive interspecific morphological variation was observed in the general structure of the gland and the glandular epithelium. Morphological variants were quantitatively scored from eight features: lobate nature of the gland; septa in the glandular epithelium; glandular epithelium having a rough or irregular outer surface; glandular epithelium having a rough or irregular inner surface; thickness of the glandular epithelium; PAS reactivity in the glandular epithelium; Periodic Acid-Schiff reactivity in the secretory product; and relative size of the cloacal scent gland. The distribution of the encoded morphological variation was compared to an established phylogeny for snakes and used as the basis for a cluster analysis. In both cases there was no apparent relationship between phylogeny and the morphological variation of the cloacal scent gland. (+info)Morphogenesis of the acrosome during the final steps of rat spermiogenesis with special reference to tubulobulbar complexes. (3/161)
We report the ultrastructural changes in acrosome morphology during the final steps of rat spermiogenesis, focusing on the relationship between the acrosome morphogenesis and the tubulobulbar complexes (TBC) development. During steps 18-19, the electron-lucent area in the dorsal cortex of the anterior acrosome gradually diminished, and finally, the acrosome became condensed and reduced its volume. Simultaneously with this tightening up of the acrosome, TBC developed from the head portion of late spermatids, protruding into the surrounding Sertoli cells. To investigate the incorporation of acrosomal contents into TBC, step 19 spermatids were stained by periodic acid-Schiff (PAS) reaction and by using the anti-acrosomal monoclonal antibody mMN7. Both PAS-reactivity and the mMN7-immunoreactivity were found in the TBC, as well as in the acrosome. In addition, the acrosome projected into the TBC-like structure, and materials of a density similar to that of the acrosome were observed in the core of the TBC. These results suggest that the TBC eliminate excess acrosomal contents prior to spermiation. (+info)Intralobar nephroblastematosis: precursor lesions of nephroblastoma in the Sprague-Dawley rat. (4/161)
Precursor lesions of spontaneous nephroblastoma (NB) in rats are here characterized for the first time, with a description of the progression of the tumor in prenatal, postnatal, and adult Sprague-Dawley rats (Upj:TUC[SD]spf.nb), which are genetically predisposed to the tumor. NB in the rat starts as a focal or multifocal interstitial accumulation of intensely basophilic immature (blastema) cells, invariably located in the deep renal cortex. Precursor lesions of NB (designated intralobar nephroblastematosis) and the early tumor do not overtly disrupt the overall structural organization and integrity of the kidney. However, with increasing size and neoplastic transformation, these lesions trap, compress, and displace/replace the existing renal tubules. Nephroblastematous foci occurred in one or both kidneys in tumor-bearing or non-tumor-bearing kidneys and in young and old rats. Like the precursor lesions, the early tumors in rats as young as 6 weeks of age were located in the inner cortex. Well-developed NB was comprised of blastema cells arranged in dense sheets or in ductular structures surrounded by mantles of blastema cells supported by varying amounts of fibromatous stroma. The stroma in one rat was hemangiosarcomatous (triphasic Wilms' tumor). Tumor cells were slightly pleomorphic and had varying amounts of granular cytoplasm with sparse organelles and showed junctional complexes and basal laminae whose frequency apparently depended upon whether the blastema cell tended to differentiate to epithelial or mesenchymal cells. NB in the rat was morphologically similar to immature pre- and postnatal kidneys, regardless of whether it occurred in young or old rats. The deep cortical location and interstitial infiltrative characteristics of precursor lesions of NB in the rat were analogous to intralobar nephrogenic rests, a variant of the precursor to Wilms' tumor in children. (+info)Cryptococcus neoformans and Mycobacterium bovis causing granulomatous pneumonia in a goat. (5/161)
An adult Blanca-Celtiberica doe originating from a goat herd with a high prevalence of tuberculosis was presented with respiratory signs. At necropsy, this goat had a diffuse and severe mycotic pneumonia associated with the presence of Cryptococcus neoformans concomitant with pulmonary focal caseous nodules from which Mycobacterium bovis was isolated. Microscopically, the mycotic lesion was a granulomatous pneumonia with many large foamy macrophages containing intracellular yeast bodies. The extensive mycotic changes, their granulomatous nature, and the lack of positive response to different immunologic tests for mycobacterial infection suggested an impaired immune status in this animal. (+info)Multiple eyelid cysts resembling apocrine hidrocystomas in three Persian cats and one Himalayan cat. (6/161)
Multiple eyelid cysts were evaluated and treated in four cats. Surgical removal of the cysts was performed in two cats. Histopathologic examination revealed multilocular cystic structures of various size. The cysts were lined by cuboidal to columnar cells with papillary or cystic projections into the cyst lumen. Periodic acid-Schiff-positive, diastase-resistant granules were seen within the apical cytoplasm of many of the cells. The eyelid masses in these cats resembled apocrine hidrocystomas in human beings, both clinically and on histopathologic examination. (+info)Re-expression of the developmental gene Pax-2 during experimental acute tubular necrosis in mice 1. (7/161)
BACKGROUND: The transcription factor Pax-2 is known to play a key regulatory role during embryonic development of the nervous and excretory systems in mammals and flies. During mouse kidney development, Pax-2 is expressed in the undifferentiated mesenchyme in response to ureter induction and continues to be expressed in the developing comma- and s-shaped bodies. These structures harbor the immediate precursors of the proximal tubular epithelial cells. Pax-2 expression is down-regulated as the differentiation of the functional units of the nephron proceeds. In the adult mammalian kidney, the Pax-2 protein is detectable exclusively in the epithelium of the collecting ducts. We sought to test the hypothesis that tissue regeneration is characterized by re-expression of developmentally important regulatory genes such as Pax-2. METHODS: The expression pattern of Pax-2 in kidneys after experimentally-induced acute tubular necrosis caused by intraperitoneally injected folic acid in mice was tested by indirect immunofluorescence, Western blotting, reverse transcriptase-polymerase chain reaction, and in situ hybridization analysis. RESULTS: A transient, temporally and locally restricted re-expression of Pax-2 in regenerating proximal tubular epithelial cells was observed following kidney damage. CONCLUSIONS: These data indicate that during the regeneration processes, developmental paradigms may be recapitulated in order to restore mature kidney function. (+info)Cell death of uterine natural killer cells in murine placenta during placentation and preterm periods. (8/161)
In the murine uterus granulated metrial gland (GMG) cells appear only during normal pregnancy. GMG cells belong to a member of natural killer (NK) cells and play an important role in fetus survival and placental growth. Our previous study revealed that mouse GMG/uterine NK (uNK) cells in the late pregnancy rapidly disappear from the uterus, due to the degenerative change classified as necrosis. But there are few reports regarding appearance and morphology of uNK cells during late pregnancy. We examined histologically and histochemically how and when uNK cells undergo cell death. The uNK cells in the metrial gland increased in number and reached maximum until day 12 of pregnancy. Sudden disappearance, however, occurred after day 15 and the granules reduced in both number and size. In situ DNA fragmentation detection revealed that DNA fragmented uNK cells increased in number during days 13 to 15 and reached 70.2% at day 15 of pregnancy. From days 13 to 17, uNK cells were positive against anti-perforin antibody. Ultrastructurally, uNK cells at day 15 showed poor organelles and unusual granules in structure. In uNK cells at day 17, condensation of nucleus chromatin, reduction in size and phagocytosis into other uNK cells were observed. These results suggested that uNK cells undergo at least two types of cell death, classified as necrosis and apoptosis, at the different stages of pregnancy, and that perforin is not a mediator for cell death. (+info)The Periodic Acid-Schiff (PAS) reaction is a histological staining method used to detect the presence of certain carbohydrates, such as glycogen and glycoproteins, in tissues or cells. This technique involves treating the tissue with periodic acid, which oxidizes the vicinal hydroxyl groups in the carbohydrates, creating aldehydes. The aldehydes then react with Schiff's reagent, forming a magenta-colored complex that is visible under a microscope.
The PAS reaction is commonly used to identify and analyze various tissue components, such as basement membranes, fungal cell walls, and mucins in the respiratory and gastrointestinal tracts. It can also be used to diagnose certain medical conditions, like kidney diseases, where abnormal accumulations of carbohydrates occur in the renal tubules or glomeruli.
In summary, the Periodic Acid-Schiff reaction is a staining method that detects specific carbohydrates in tissues or cells, which can aid in diagnostic and research applications.
Alcian Blue is a type of dye that is commonly used in histology, which is the study of the microscopic structure of tissues. It is particularly useful for staining acidic mucopolysaccharides and proteoglycans, which are important components of the extracellular matrix in many tissues.
Alcian Blue binds to these negatively charged molecules through ionic interactions, forming a complex that can be visualized under a microscope. The dye is often used in combination with other stains to provide contrast and highlight specific structures within tissues.
The intensity of the Alcian Blue stain can also provide information about the degree of sulfation or carboxylation of the mucopolysaccharides, which can be useful in diagnosing certain diseases or abnormalities. For example, changes in the staining pattern of proteoglycans have been associated with various types of arthritis and other joint disorders.
Overall, Alcian Blue is an important tool in the field of histology and has contributed significantly to our understanding of tissue structure and function.
A Schiff base is not a medical term per se, but rather a chemical concept that can be relevant in various scientific and medical fields. A Schiff base is a chemical compound that contains a carbon-nitrogen double bond with the nitrogen atom connected to an aryl or alkyl group, excluding hydrogen. This structure is also known as an azomethine.
The general formula for a Schiff base is R1R2C=NR3, where R1 and R2 are organic groups (aryl or alkyl), and R3 is a hydrogen atom or an organic group. These compounds can be synthesized by the condensation of a primary amine with a carbonyl compound, such as an aldehyde or ketone.
Schiff bases have been studied in various medical and biological contexts due to their potential bioactivities. Some Schiff bases exhibit antimicrobial, antifungal, anti-inflammatory, and anticancer properties. They can also serve as ligands for metal ions, forming complexes with potential applications in medicinal chemistry, such as in the development of new drugs or diagnostic agents.
Sialomucins are a type of glycoprotein mucins that contain high amounts of sialic acid, which is a family of negatively charged sugars found on the surface of many cell types. These mucins are produced by the major salivary glands and are a major component of saliva. They play an important role in lubricating and protecting the oral cavity, as well as contributing to the mouth's ability to resist infection and damage.
Sialomucins have also been shown to have various biological functions, such as regulating cell adhesion, modulating immune responses, and serving as receptors for certain viruses and bacteria. Abnormalities in sialomucin expression or structure have been implicated in several diseases, including cancer, autoimmune disorders, and infectious diseases.
Hematoxylin is not a medical term per se, but it is widely used in the field of histology and pathology, which are subspecialties within medicine. Hematoxylin is a natural dye that is commonly used in histological staining procedures to highlight cell nuclei in tissue samples. It is often combined with eosin, another dye, to create the well-known hematoxylin and eosin (H&E) stain, which is routinely used to examine tissue architecture and diagnose various medical conditions.
In essence, hematoxylin is a histological stain that selectively binds to the acidic components of nuclear chromatin, imparting a blue-purple color to the cell nuclei when visualized under a microscope. This staining technique helps pathologists and researchers identify and analyze various cellular structures and abnormalities within tissue samples.
Histochemistry is the branch of pathology that deals with the microscopic localization of cellular or tissue components using specific chemical reactions. It involves the application of chemical techniques to identify and locate specific biomolecules within tissues, cells, and subcellular structures. This is achieved through the use of various staining methods that react with specific antigens or enzymes in the sample, allowing for their visualization under a microscope. Histochemistry is widely used in diagnostic pathology to identify different types of tissues, cells, and structures, as well as in research to study cellular and molecular processes in health and disease.
Periodic acid is not a medical term per se, but it is a chemical reagent that is used in some laboratory tests and staining procedures in the field of pathology, which is a medical specialty.
Periodic acid is an oxidizing agent with the chemical formula HIO4 or H5IO6. It is often used in histology (the study of the microscopic structure of tissues) to perform a special staining technique called the periodic acid-Schiff (PAS) reaction. This reaction is used to identify certain types of carbohydrates, such as glycogen and some types of mucins, in tissues.
The periodic acid first oxidizes the carbohydrate molecules, creating aldehydes. These aldehydes then react with a Schiff reagent, which results in a pink or magenta color. This reaction can help pathologists identify and diagnose various medical conditions, such as cancer, infection, and inflammation.
Mucins are high molecular weight, heavily glycosylated proteins that are the major components of mucus. They are produced and secreted by specialized epithelial cells in various organs, including the respiratory, gastrointestinal, and urogenital tracts, as well as the eyes and ears.
Mucins have a characteristic structure consisting of a protein backbone with numerous attached oligosaccharide side chains, which give them their gel-forming properties and provide a protective barrier against pathogens, environmental insults, and digestive enzymes. They also play important roles in lubrication, hydration, and cell signaling.
Mucins can be classified into two main groups based on their structure and function: secreted mucins and membrane-bound mucins. Secreted mucins are released from cells and form a physical barrier on the surface of mucosal tissues, while membrane-bound mucins are integrated into the cell membrane and participate in cell adhesion and signaling processes.
Abnormalities in mucin production or function have been implicated in various diseases, including chronic inflammation, cancer, and cystic fibrosis.
Mucus is a viscous, slippery secretion produced by the mucous membranes that line various body cavities such as the respiratory and gastrointestinal tracts. It serves to lubricate and protect these surfaces from damage, infection, and foreign particles. Mucus contains water, proteins, salts, and other substances, including antibodies, enzymes, and glycoproteins called mucins that give it its characteristic gel-like consistency.
In the respiratory system, mucus traps inhaled particles such as dust, allergens, and pathogens, preventing them from reaching the lungs. The cilia, tiny hair-like structures lining the airways, move the mucus upward toward the throat, where it can be swallowed or expelled through coughing or sneezing. In the gastrointestinal tract, mucus helps protect the lining of the stomach and intestines from digestive enzymes and other harmful substances.
Excessive production of mucus can occur in various medical conditions such as allergies, respiratory infections, chronic lung diseases, and gastrointestinal disorders, leading to symptoms such as coughing, wheezing, nasal congestion, and diarrhea.
'Staining and labeling' are techniques commonly used in pathology, histology, cytology, and molecular biology to highlight or identify specific components or structures within tissues, cells, or molecules. These methods enable researchers and medical professionals to visualize and analyze the distribution, localization, and interaction of biological entities, contributing to a better understanding of diseases, cellular processes, and potential therapeutic targets.
Medical definitions for 'staining' and 'labeling' are as follows:
1. Staining: A process that involves applying dyes or stains to tissues, cells, or molecules to enhance their contrast and reveal specific structures or components. Stains can be categorized into basic stains (which highlight acidic structures) and acidic stains (which highlight basic structures). Common staining techniques include Hematoxylin and Eosin (H&E), which differentiates cell nuclei from the surrounding cytoplasm and extracellular matrix; special stains, such as PAS (Periodic Acid-Schiff) for carbohydrates or Masson's trichrome for collagen fibers; and immunostains, which use antibodies to target specific proteins.
2. Labeling: A process that involves attaching a detectable marker or tag to a molecule of interest, allowing its identification, quantification, or tracking within a biological system. Labels can be direct, where the marker is directly conjugated to the targeting molecule, or indirect, where an intermediate linker molecule is used to attach the label to the target. Common labeling techniques include fluorescent labels (such as FITC, TRITC, or Alexa Fluor), enzymatic labels (such as horseradish peroxidase or alkaline phosphatase), and radioactive labels (such as ³²P or ¹⁴C). Labeling is often used in conjunction with staining techniques to enhance the specificity and sensitivity of detection.
Together, staining and labeling provide valuable tools for medical research, diagnostics, and therapeutic development, offering insights into cellular and molecular processes that underlie health and disease.
Electrophoresis, polyacrylamide gel (EPG) is a laboratory technique used to separate and analyze complex mixtures of proteins or nucleic acids (DNA or RNA) based on their size and electrical charge. This technique utilizes a matrix made of cross-linked polyacrylamide, a type of gel, which provides a stable and uniform environment for the separation of molecules.
In this process:
1. The polyacrylamide gel is prepared by mixing acrylamide monomers with a cross-linking agent (bis-acrylamide) and a catalyst (ammonium persulfate) in the presence of a buffer solution.
2. The gel is then poured into a mold and allowed to polymerize, forming a solid matrix with uniform pore sizes that depend on the concentration of acrylamide used. Higher concentrations result in smaller pores, providing better resolution for separating smaller molecules.
3. Once the gel has set, it is placed in an electrophoresis apparatus containing a buffer solution. Samples containing the mixture of proteins or nucleic acids are loaded into wells on the top of the gel.
4. An electric field is applied across the gel, causing the negatively charged molecules to migrate towards the positive electrode (anode) while positively charged molecules move toward the negative electrode (cathode). The rate of migration depends on the size, charge, and shape of the molecules.
5. Smaller molecules move faster through the gel matrix and will migrate farther from the origin compared to larger molecules, resulting in separation based on size. Proteins and nucleic acids can be selectively stained after electrophoresis to visualize the separated bands.
EPG is widely used in various research fields, including molecular biology, genetics, proteomics, and forensic science, for applications such as protein characterization, DNA fragment analysis, cloning, mutation detection, and quality control of nucleic acid or protein samples.
Carbohydrates are a major nutrient class consisting of organic compounds that primarily contain carbon, hydrogen, and oxygen atoms. They are classified as saccharides, which include monosaccharides (simple sugars), disaccharides (double sugars), oligosaccharides (short-chain sugars), and polysaccharides (complex carbohydrates).
Monosaccharides, such as glucose, fructose, and galactose, are the simplest form of carbohydrates. They consist of a single sugar molecule that cannot be broken down further by hydrolysis. Disaccharides, like sucrose (table sugar), lactose (milk sugar), and maltose (malt sugar), are formed from two monosaccharide units joined together.
Oligosaccharides contain a small number of monosaccharide units, typically less than 20, while polysaccharides consist of long chains of hundreds to thousands of monosaccharide units. Polysaccharides can be further classified into starch (found in plants), glycogen (found in animals), and non-starchy polysaccharides like cellulose, chitin, and pectin.
Carbohydrates play a crucial role in providing energy to the body, with glucose being the primary source of energy for most cells. They also serve as structural components in plants (cellulose) and animals (chitin), participate in various metabolic processes, and contribute to the taste, texture, and preservation of foods.
List of MeSH codes (E05)
Periodic acid-Schiff stain
Onychomycosis
Schiff test
Zygomycosis
Acral myxoinflammatory fibroblastic sarcoma
Diastase
Periodate
Pneumocystis pneumonia
Histopathologic diagnosis of dermatitis
Kerion
Bismarck brown Y
Hexamethylenetetramine
Emmonsia parva
Staining
List of organic reactions
Chromoblastomycosis
Pathology of multiple sclerosis
Glycogen storage disease type 0
Extended periodic table
Glycophorin C
Oral candidiasis
Cellular automaton
Perineuronal net
Bioremediation
Coniine
Radium
Manganese
List of people from Italy
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MESH TREE NUMBER CHANGES - 2012 MeSH. August 19, 2011
Stain9
- Cytoplasmic inclusions did not stain with the periodic acid-Schiff (PAS) reaction. (bvsalud.org)
- Note metachromatic staining (reddish to purple color reaction with a blue stain). (oncohemakey.com)
- Periodic acid Schiff stain. (oncohemakey.com)
- Leukemic erythroblasts characteristically react with this stain with a reddish colored reaction product. (oncohemakey.com)
- Histopathologic examination of nail clippings with a periodic acid-Schiff (PAS) stain is a method for confirming the diagnosis for patients with suspected onychomycosis , a fungal nail infection most often caused by dermatophytes. (cdc.gov)
- To determine the properties of the secretions of the ceruminous glands in the meatus acusticus externus of cats and dogs, the periodic acid Schiff (PAS) stain was used for neutral mucosubstances and glycogen, while the Alcian blue stain (pH 2.5) was applied for the presence of acidic mucosubstances. (tubitak.gov.tr)
- The Alcian blue stain (pH 2.5) for acidic mucosubstances gave positive reactions for cats and negative reactions for dogs. (tubitak.gov.tr)
- The organisms are gram negative, and highlighted with Giemsa and Macchiavello stains, but do not stain with periodic acid-Schiff. (vin.com)
- Periodic Acid Schiff (PAS) stain highlights(fuchsia) the vascular walls and (asterix line) and the branching fungus invading(arrow heads) the vascular lumen(L). Diffuse necrosis and poor inflammatory reaction in the background with destruction of normal anatomy. (eyewiki.org)
Reagent4
- The resulting aldehydes react with Schiff reagent to form a colored product. (umassmed.edu)
- Periodic acid-Schiff reaction (PAS) The Schiff reagent is a bleached basic fuschin that reacts with aldehyde groups. (feministlawprofessors.com)
- The reaction of periodic acid selectively oxidizes the glucose residues, creates aldehydes that react with the Schiff reagent and creates a purple-magenta color. (wikidoc.org)
- Periodic Acid/Schiff Reagent (PAS) Staining. (edrc2013.org)
Polymerase7
- Reliable diagnosis based on a combination of periodic acid-Schiff (PAS) staining, T. whipplei-specific immunohistochemistry (IHC), and polymerase chain reaction (PCR) from duode-nal biopsies may be challenging in cases without classical gastrointestinal infection, so the need for additional diag-nostic materials is urgent. (123dok.net)
- RpoB gene polymerase chain reaction (PCR) can be an additional tool to assist in the diagnosis of Whipple's disease. (123dok.net)
- Polymerase chain reaction (PCR) is a quick and increasingly used method for diagnosing ringworm. (cdc.gov)
- Investigations include smears, biopsy, staining with periodic acid-Schiff (PAS) or Gomori methenamine silver, culture of the affected tissues, polymerase chain reaction (PCR), serodiagnosis (sometimes), physical examination, and chest radiography. (medscape.com)
- Polymerase chain reactions (PCR) and Sanger sequencing were performed on samples from one pup. (vin.com)
- At the end of 8 weeks, all rats were sacrificed to obtain the kidney tissues for histological examination and reverse transcription polymerase chain reaction (RT-PCR) was used to analyze the transcriptional levels of nephrin and podocin genes. (biomedcentral.com)
- Deoxyribonucleic acid from FFPE tissue samples and control blood samples was amplified by high-resolution melt real-time polymerase chain reaction before sequencing. (bvsalud.org)
Staining5
- Whipple's disease (WD) is a rare chronic systemic infection with a wide range of clinical symptoms, routinely diagnosed in biopsies from the small intestine and other tissues by periodic acid-Schiff (PAS) diastase staining and immunohistological analysis with specific antibodies. (frontiersin.org)
- Glycogen concentrations were measured through periodic acid Schiff's staining (PAS) method and by using an enzymatic detection kit, in various time points. (concordia.ca)
- immunohistochemistry (IHC) and periodic acid- Schiff (PAS) staining. (123dok.net)
- In dogs, positive staining was observed for glycogen and neutral mucosubstances, whereas, in cats, negative reactions were observed. (tubitak.gov.tr)
- Periodic acid-Schiff ( PAS ) is a staining method used in histology and pathology . (wikidoc.org)
Stains5
- Periodic acid-Schiff and mucicarmine stains showed negative reaction. (jnsbm.org)
- Periodic Acid-Schiff, Acid-Fast Bacillus and Gram stains were negative for microorganisms. (juniperpublishers.com)
- Histologic sections were stained with hematoxylin-eosin, periodic acid-Schiff, and Masson trichrome stains. (cumhuriyet.edu.tr)
- While some of these are special fungal stains, others are of more general use but still help to observe the tissue reactions and/or the infecting fungus. (drfungus.org)
- The reactions involved and comments of particular interest for some of these stains are summarized in the TABLE. (drfungus.org)
Tissues1
- Chemotherapy with CP disrupts reductive reactions in tissues and creates oxidative stress (1,3) as an alkylating agent, finally reducing fertilization in patients under treatment (4,5). (ijfs.ir)
Glycogen2
- The diastase reaction was applied to confirm the presence of glycogen. (tubitak.gov.tr)
- The positive reactions observed in dogs were found not to originate from glycogen by the negative diastase reactions observed. (tubitak.gov.tr)
Methenamine silver1
- Gomori methenamine silver (GMS), Gridley's fungus (GF), and periodic acid-Schiff (PAS) are special for and very efficient to visualize the fungi. (drfungus.org)
Biopsy2
- a positive periodic acid-Schiff (PAS) reaction in macro- phages from a small bowel biopsy sample ( 2 ). (cdc.gov)
- Excision biopsy of deep cut sections of the lesion revealed sulfur granules with a positive periodic acid Schiff reaction and Mycetoma is caused by fungi or fungus- eumycotic mycetoma was suggested. (who.int)
Fungal1
- Calcium oxalate crystals, probably originating from fungal oxalic acid, are present near hyphae in necrotic tissue, if the infecting species is Aspergillus niger . (drfungus.org)
Nucleic1
- A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes. (uams.edu)
Lipid1
- After 4 hr, the reaction was stopped with MeOH/1 N HCl (1:1), and the lipid was extracted with chloroform. (edrc2013.org)
Inflammatory1
- Histologic sections did not reveal inflammatory reaction, cell degeneration, and cell necrosis. (cumhuriyet.edu.tr)
Diagnosis1
- The mechanisms by which isocyanates cause asthma remain unclear, hampering disease prevention, diagnosis, and treatment.7 It is assumed the reactive nature of N=C=O groups underlies isocyanate asthma, with a hapten-based mechanism as the most obvious pathway to pathogenesis.8 However, the critical "self" reaction targets for isocyanate in vivo remain uncertain. (cdc.gov)
Positive1
- Positive reaction (black reaction product) in myeloblasts and immature eosinophils. (oncohemakey.com)
Tissue2
- The major advantages of histopathology are speed, low-cost, and the ability to provide a presumptive identification of the infecting fungus, as well as demonstrating the tissue reaction. (drfungus.org)
- Thus, a combination of GMS and H&E is usually employed to visualize both the tissue reaction and the infecting fungus. (drfungus.org)
Antibodies1
- The neoplastic cells showed complete negative reaction with antibodies for vimentin, smooth muscle actin and homatropine bromide-45 antigens. (jnsbm.org)
Clinical Trials1
- 3, 4 Several tumour vaccines directed against MUC1 core protein, oligosaccharides, or MUC1 cDNAs have been shown to stimulate immune reactions in basic clinical trials to confer antitumour activity. (bmj.com)
Biopsies1
- The biopsies of both sites showed findings consistent with psoriasiform dermatitis and were both periodic acid-Schiff (PAS) negative ( Figure ). (consultant360.com)
Cells1
- The cultured cells on a glass slide were reacted with PAS Rifamdin after periodic treatment. (edrc2013.org)
Major1
- This graph shows the total number of publications written about "Periodic Acid-Schiff Reaction" by people in this website by year, and whether "Periodic Acid-Schiff Reaction" was a major or minor topic of these publications. (umassmed.edu)
Shows1
- The cereal market shows a more elastic reaction to price responses, even though demand for staple foods is generally inelastic. (bvsalud.org)
Important1
- The paper presents the reaction of the Romanian cereal market to the disruption of trade flows caused by certain shocks, such as the COVID-19 pandemic, which lead to changes with high impact on the functioning of this market, representing an important test for the resilience of the sector. (bvsalud.org)
Blue1
- Paraffin sections were stained with either an aqueous mixture of alcian blue 8GX, safaranin 0 and Bismark brown Y, or with the periodic acid - Schiff (PAS) reaction. (usda.gov)
Prognosis2
Polymerase chain re2
- Whipple's disease may be diagnosed by periodic acid-Schiff (PAS) staining, electron microscopy, or polymerase chain reaction of intestinal biopsy specimens. (nih.gov)
- Onychomycosis infections: Do polymerase chain reaction and culture reports agree? (msdmanuals.com)
Electron1
- Hematoxylin and Eosin, periodic acid-Schiff, inducible nitric oxide synthase, proliferating cell nuclear antigen expression and electron microscopy investigation were performed. (ijpsonline.com)
Negatively1
- These rods contain the Z-line protein, α -actinin, that can be effectively stained in skeletal muscles using Gomori or Masson trichrome and negatively stained with periodic acid-Schiff. (scielo.org.za)
Amino acids3
- This sweetener is a dipeptide derived from the combination of two non-aromatic amino acids namely, aspartic acids and phenylalanine. (ijfs.ir)
- After oral intake, aspartame is hydrolyzed in the gastrointestinal tract by esterases and peptidases into amino acids (aspartic acid and phenylalanine) and methanol. (ijfs.ir)
- Investigators reported that L-carnitine is a compound biosynthesized from the amino acids methionine, and lysine in kidney and liver. (ijpsonline.com)
Lichenoid2
- OBJECTIVES: Lichenoid granulomatous reaction can be subcategorized into lichenoid granulomatous dermatitis or lichenoid granulomatous stomatitis. (elsevierpure.com)
- METHODS AND MATERIALS: We retrieved all cases diagnosed with lichenoid reaction with granulomatous inflammation between January 1, 2000, and August 1, 2016, from the University of Florida Oral Pathology Biopsy Service Archives. (elsevierpure.com)
Differentiation1
- 7. Extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue with plasma cell differentiation: Periodic acid-schiff reaction-positive Dutcher body is a diagnostic clue to distinguish it from plasmacytoma. (nih.gov)
Fungus1
- Excision biopsy of deep cut sections of the lesion revealed sulfur granules with a positive periodic acid Schiff reaction and Mycetoma is caused by fungi or fungus- eumycotic mycetoma was suggested. (who.int)
Schiff's1
- The neutrophilic granulocytes (NG) and monocytes functional activity, the content of myeloperoxidase and substances with a positive reaction to Schiff's periodic acid (PAS- stained substances), the cytokines content were determined: interleukin-1β (IL-1β), IL-2, IL-4, IL- 6, tumor necrosis factor-α (TNF-α). (kiev.ua)
Glycogen3
- It is also significant in life science applications as the Periodic Acid-Schiff reaction for the analysis of intracellular polysaccharides like glycogen, starch and cellulose. (williamblythe.com)
- The periodic acid-Schiff reaction showed the presence of higher glycogen content material (pink) in cells from FCS and LIF conditions but considerably lower levels in the CNTF condition. (dhfrinhibitor.com)
- For LIF-differentiated astrocytes, glycogen levels remained elevated for two h and decreasedCytologic Reaction for Glycogen and ImmunocytochemistryCytologic localization of glycogen was performed working with the periodic acid-Schiff method (Rosenberg and Dichter, 1985). (dhfrinhibitor.com)
Chemical2
- Naphthalene toxicity is the result of the biotransformation process, which consists in cellular chemical reactions with the aim of making the compounds more soluble in water, facilitating their excretion. (scielo.br)
- Aristolochic acids (AAs) are chemical compounds that are also known as nitrophenanthrene carboxylic acid compounds. (biomedcentral.com)
Product1
- The resulting aldehydes react with Schiff reagent to form a colored product. (nih.gov)
Long1
- L-carnitine assists as a cofactor necessary for the transport of long chain fatty acids into the mitochondria for energy production in the peripheral tissues[ 7 ]. (ijpsonline.com)
Base1
- It functions in maintaining acid base balance and constant intraocular temperature [4,5]. (heraldopenaccess.us)
Significant1
- There is a significant reduction in nephrotoxicity and infusion-related reactions with continuous 24-h infusion vs conventional 2-h to 6-h infusion. (medscape.com)
Study1
- In this study, we established an aristolochic acid nephropathy (AAN) model by intraperitoneally administering aristolochic acid I sodium salt (AA-I) at a dose of 5 mg/kg body weight on alternate days for 4 weeks. (biomedcentral.com)
Levels2
- Our unique production technology uses a proprietary multi-stage process that reduces trace impurities to ppb levels and allows us to produce large volumes of very high purity periodic acid. (williamblythe.com)
- William Blythe understands that impurity levels are critical to many of the applications of periodic acid and is continually investing to reduce trace impurity levels to meet customer needs. (williamblythe.com)