A histochemical technique for staining carbohydrates. It is based on PERIODIC ACID oxidation of a substance containing adjacent hydroxyl groups. The resulting aldehydes react with Schiff reagent to form a colored product.
A copper-containing dye used as a gelling agent for lubricants, for staining of bacteria and for the dyeing of histiocytes and fibroblasts in vivo.
Condensation products of aromatic amines and aldehydes forming azomethines substituted on the N atom, containing the general formula R-N:CHR. (From Grant & Hackh's Chemical Dictionary, 5th ed)
A subcategory of mucins that contain SIALIC ACID.
A dye obtained from the heartwood of logwood (Haematoxylon campechianum Linn., Leguminosae) used as a stain in microscopy and in the manufacture of ink.
Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.
A strong oxidizing agent.
High molecular weight mucoproteins that protect the surface of EPITHELIAL CELLS by providing a barrier to particulate matter and microorganisms. Membrane-anchored mucins may have additional roles concerned with protein interactions at the cell surface.
The viscous secretion of mucous membranes. It contains mucin, white blood cells, water, inorganic salts, and exfoliated cells.
The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.

Amylopectinosis in fetal and neonatal Quarter Horses. (1/161)

Three Quarter Horses, a stillborn filly (horse No. 1), a female fetus aborted at approximately 6 months of gestation (horse No. 2), and a 1-month-old colt that had been weak at birth (horse No. 3), had myopathy characterized histologically by large spherical or ovoid inclusions in skeletal and cardiac myofibers. Smaller inclusions were also found in brain and spinal cord and in some cells of all other tissues examined. These inclusions were basophilic, red-purple after staining with periodic acid-Schiff (both before and after digestion with diastase), and moderately dark blue after staining with toluidine blue. The inclusions did not react when stained with Congo red. Staining with iodine ranged from pale blue to black. Their ultrastructural appearance varied from amorphous to somewhat filamentous. On the basis of staining characteristics and diastase resistance, we concluded that these inclusions contained amylopectin. A distinctly different kind of inclusion material was also present in skeletal muscle and tongue of horse Nos. 1 and 3. These inclusions were crystalline with a sharply defined ultrastructural periodicity. The crystals were eosinophilic and very dark blue when stained with toluidine blue but did not stain with iodine. Crystals sometimes occurred freely within the myofibers but more often were encased by deposits of amylopectin. This combination of histologic and ultrastructural features characterizes a previously unreported storage disease in fetal and neonatal Quarter Horses, with findings similar to those of glycogen storage disease type IV. We speculate that a severe inherited loss of glycogen brancher enzyme activity may be responsible for these findings. The relation of amylopectinosis to the death of the foals is unknown.  (+info)

Comparative morphology of the cloacal scent gland in snakes (Serpentes: Reptilia). (2/161)

The histology of the cloacal scent gland, or anal gland, was examined from a diverse group of 50 snakes. Extensive interspecific morphological variation was observed in the general structure of the gland and the glandular epithelium. Morphological variants were quantitatively scored from eight features: lobate nature of the gland; septa in the glandular epithelium; glandular epithelium having a rough or irregular outer surface; glandular epithelium having a rough or irregular inner surface; thickness of the glandular epithelium; PAS reactivity in the glandular epithelium; Periodic Acid-Schiff reactivity in the secretory product; and relative size of the cloacal scent gland. The distribution of the encoded morphological variation was compared to an established phylogeny for snakes and used as the basis for a cluster analysis. In both cases there was no apparent relationship between phylogeny and the morphological variation of the cloacal scent gland.  (+info)

Morphogenesis of the acrosome during the final steps of rat spermiogenesis with special reference to tubulobulbar complexes. (3/161)

We report the ultrastructural changes in acrosome morphology during the final steps of rat spermiogenesis, focusing on the relationship between the acrosome morphogenesis and the tubulobulbar complexes (TBC) development. During steps 18-19, the electron-lucent area in the dorsal cortex of the anterior acrosome gradually diminished, and finally, the acrosome became condensed and reduced its volume. Simultaneously with this tightening up of the acrosome, TBC developed from the head portion of late spermatids, protruding into the surrounding Sertoli cells. To investigate the incorporation of acrosomal contents into TBC, step 19 spermatids were stained by periodic acid-Schiff (PAS) reaction and by using the anti-acrosomal monoclonal antibody mMN7. Both PAS-reactivity and the mMN7-immunoreactivity were found in the TBC, as well as in the acrosome. In addition, the acrosome projected into the TBC-like structure, and materials of a density similar to that of the acrosome were observed in the core of the TBC. These results suggest that the TBC eliminate excess acrosomal contents prior to spermiation.  (+info)

Intralobar nephroblastematosis: precursor lesions of nephroblastoma in the Sprague-Dawley rat. (4/161)

Precursor lesions of spontaneous nephroblastoma (NB) in rats are here characterized for the first time, with a description of the progression of the tumor in prenatal, postnatal, and adult Sprague-Dawley rats (Upj:TUC[SD]spf.nb), which are genetically predisposed to the tumor. NB in the rat starts as a focal or multifocal interstitial accumulation of intensely basophilic immature (blastema) cells, invariably located in the deep renal cortex. Precursor lesions of NB (designated intralobar nephroblastematosis) and the early tumor do not overtly disrupt the overall structural organization and integrity of the kidney. However, with increasing size and neoplastic transformation, these lesions trap, compress, and displace/replace the existing renal tubules. Nephroblastematous foci occurred in one or both kidneys in tumor-bearing or non-tumor-bearing kidneys and in young and old rats. Like the precursor lesions, the early tumors in rats as young as 6 weeks of age were located in the inner cortex. Well-developed NB was comprised of blastema cells arranged in dense sheets or in ductular structures surrounded by mantles of blastema cells supported by varying amounts of fibromatous stroma. The stroma in one rat was hemangiosarcomatous (triphasic Wilms' tumor). Tumor cells were slightly pleomorphic and had varying amounts of granular cytoplasm with sparse organelles and showed junctional complexes and basal laminae whose frequency apparently depended upon whether the blastema cell tended to differentiate to epithelial or mesenchymal cells. NB in the rat was morphologically similar to immature pre- and postnatal kidneys, regardless of whether it occurred in young or old rats. The deep cortical location and interstitial infiltrative characteristics of precursor lesions of NB in the rat were analogous to intralobar nephrogenic rests, a variant of the precursor to Wilms' tumor in children.  (+info)

Cryptococcus neoformans and Mycobacterium bovis causing granulomatous pneumonia in a goat. (5/161)

An adult Blanca-Celtiberica doe originating from a goat herd with a high prevalence of tuberculosis was presented with respiratory signs. At necropsy, this goat had a diffuse and severe mycotic pneumonia associated with the presence of Cryptococcus neoformans concomitant with pulmonary focal caseous nodules from which Mycobacterium bovis was isolated. Microscopically, the mycotic lesion was a granulomatous pneumonia with many large foamy macrophages containing intracellular yeast bodies. The extensive mycotic changes, their granulomatous nature, and the lack of positive response to different immunologic tests for mycobacterial infection suggested an impaired immune status in this animal.  (+info)

Multiple eyelid cysts resembling apocrine hidrocystomas in three Persian cats and one Himalayan cat. (6/161)

Multiple eyelid cysts were evaluated and treated in four cats. Surgical removal of the cysts was performed in two cats. Histopathologic examination revealed multilocular cystic structures of various size. The cysts were lined by cuboidal to columnar cells with papillary or cystic projections into the cyst lumen. Periodic acid-Schiff-positive, diastase-resistant granules were seen within the apical cytoplasm of many of the cells. The eyelid masses in these cats resembled apocrine hidrocystomas in human beings, both clinically and on histopathologic examination.  (+info)

Re-expression of the developmental gene Pax-2 during experimental acute tubular necrosis in mice 1. (7/161)

BACKGROUND: The transcription factor Pax-2 is known to play a key regulatory role during embryonic development of the nervous and excretory systems in mammals and flies. During mouse kidney development, Pax-2 is expressed in the undifferentiated mesenchyme in response to ureter induction and continues to be expressed in the developing comma- and s-shaped bodies. These structures harbor the immediate precursors of the proximal tubular epithelial cells. Pax-2 expression is down-regulated as the differentiation of the functional units of the nephron proceeds. In the adult mammalian kidney, the Pax-2 protein is detectable exclusively in the epithelium of the collecting ducts. We sought to test the hypothesis that tissue regeneration is characterized by re-expression of developmentally important regulatory genes such as Pax-2. METHODS: The expression pattern of Pax-2 in kidneys after experimentally-induced acute tubular necrosis caused by intraperitoneally injected folic acid in mice was tested by indirect immunofluorescence, Western blotting, reverse transcriptase-polymerase chain reaction, and in situ hybridization analysis. RESULTS: A transient, temporally and locally restricted re-expression of Pax-2 in regenerating proximal tubular epithelial cells was observed following kidney damage. CONCLUSIONS: These data indicate that during the regeneration processes, developmental paradigms may be recapitulated in order to restore mature kidney function.  (+info)

Cell death of uterine natural killer cells in murine placenta during placentation and preterm periods. (8/161)

In the murine uterus granulated metrial gland (GMG) cells appear only during normal pregnancy. GMG cells belong to a member of natural killer (NK) cells and play an important role in fetus survival and placental growth. Our previous study revealed that mouse GMG/uterine NK (uNK) cells in the late pregnancy rapidly disappear from the uterus, due to the degenerative change classified as necrosis. But there are few reports regarding appearance and morphology of uNK cells during late pregnancy. We examined histologically and histochemically how and when uNK cells undergo cell death. The uNK cells in the metrial gland increased in number and reached maximum until day 12 of pregnancy. Sudden disappearance, however, occurred after day 15 and the granules reduced in both number and size. In situ DNA fragmentation detection revealed that DNA fragmented uNK cells increased in number during days 13 to 15 and reached 70.2% at day 15 of pregnancy. From days 13 to 17, uNK cells were positive against anti-perforin antibody. Ultrastructurally, uNK cells at day 15 showed poor organelles and unusual granules in structure. In uNK cells at day 17, condensation of nucleus chromatin, reduction in size and phagocytosis into other uNK cells were observed. These results suggested that uNK cells undergo at least two types of cell death, classified as necrosis and apoptosis, at the different stages of pregnancy, and that perforin is not a mediator for cell death.  (+info)

The Periodic Acid-Schiff (PAS) reaction is a histological staining method used to detect the presence of certain carbohydrates, such as glycogen and glycoproteins, in tissues or cells. This technique involves treating the tissue with periodic acid, which oxidizes the vicinal hydroxyl groups in the carbohydrates, creating aldehydes. The aldehydes then react with Schiff's reagent, forming a magenta-colored complex that is visible under a microscope.

The PAS reaction is commonly used to identify and analyze various tissue components, such as basement membranes, fungal cell walls, and mucins in the respiratory and gastrointestinal tracts. It can also be used to diagnose certain medical conditions, like kidney diseases, where abnormal accumulations of carbohydrates occur in the renal tubules or glomeruli.

In summary, the Periodic Acid-Schiff reaction is a staining method that detects specific carbohydrates in tissues or cells, which can aid in diagnostic and research applications.

Alcian Blue is a type of dye that is commonly used in histology, which is the study of the microscopic structure of tissues. It is particularly useful for staining acidic mucopolysaccharides and proteoglycans, which are important components of the extracellular matrix in many tissues.

Alcian Blue binds to these negatively charged molecules through ionic interactions, forming a complex that can be visualized under a microscope. The dye is often used in combination with other stains to provide contrast and highlight specific structures within tissues.

The intensity of the Alcian Blue stain can also provide information about the degree of sulfation or carboxylation of the mucopolysaccharides, which can be useful in diagnosing certain diseases or abnormalities. For example, changes in the staining pattern of proteoglycans have been associated with various types of arthritis and other joint disorders.

Overall, Alcian Blue is an important tool in the field of histology and has contributed significantly to our understanding of tissue structure and function.

A Schiff base is not a medical term per se, but rather a chemical concept that can be relevant in various scientific and medical fields. A Schiff base is a chemical compound that contains a carbon-nitrogen double bond with the nitrogen atom connected to an aryl or alkyl group, excluding hydrogen. This structure is also known as an azomethine.

The general formula for a Schiff base is R1R2C=NR3, where R1 and R2 are organic groups (aryl or alkyl), and R3 is a hydrogen atom or an organic group. These compounds can be synthesized by the condensation of a primary amine with a carbonyl compound, such as an aldehyde or ketone.

Schiff bases have been studied in various medical and biological contexts due to their potential bioactivities. Some Schiff bases exhibit antimicrobial, antifungal, anti-inflammatory, and anticancer properties. They can also serve as ligands for metal ions, forming complexes with potential applications in medicinal chemistry, such as in the development of new drugs or diagnostic agents.

Sialomucins are a type of glycoprotein mucins that contain high amounts of sialic acid, which is a family of negatively charged sugars found on the surface of many cell types. These mucins are produced by the major salivary glands and are a major component of saliva. They play an important role in lubricating and protecting the oral cavity, as well as contributing to the mouth's ability to resist infection and damage.

Sialomucins have also been shown to have various biological functions, such as regulating cell adhesion, modulating immune responses, and serving as receptors for certain viruses and bacteria. Abnormalities in sialomucin expression or structure have been implicated in several diseases, including cancer, autoimmune disorders, and infectious diseases.

Hematoxylin is not a medical term per se, but it is widely used in the field of histology and pathology, which are subspecialties within medicine. Hematoxylin is a natural dye that is commonly used in histological staining procedures to highlight cell nuclei in tissue samples. It is often combined with eosin, another dye, to create the well-known hematoxylin and eosin (H&E) stain, which is routinely used to examine tissue architecture and diagnose various medical conditions.

In essence, hematoxylin is a histological stain that selectively binds to the acidic components of nuclear chromatin, imparting a blue-purple color to the cell nuclei when visualized under a microscope. This staining technique helps pathologists and researchers identify and analyze various cellular structures and abnormalities within tissue samples.

Histochemistry is the branch of pathology that deals with the microscopic localization of cellular or tissue components using specific chemical reactions. It involves the application of chemical techniques to identify and locate specific biomolecules within tissues, cells, and subcellular structures. This is achieved through the use of various staining methods that react with specific antigens or enzymes in the sample, allowing for their visualization under a microscope. Histochemistry is widely used in diagnostic pathology to identify different types of tissues, cells, and structures, as well as in research to study cellular and molecular processes in health and disease.

Periodic acid is not a medical term per se, but it is a chemical reagent that is used in some laboratory tests and staining procedures in the field of pathology, which is a medical specialty.

Periodic acid is an oxidizing agent with the chemical formula HIO4 or H5IO6. It is often used in histology (the study of the microscopic structure of tissues) to perform a special staining technique called the periodic acid-Schiff (PAS) reaction. This reaction is used to identify certain types of carbohydrates, such as glycogen and some types of mucins, in tissues.

The periodic acid first oxidizes the carbohydrate molecules, creating aldehydes. These aldehydes then react with a Schiff reagent, which results in a pink or magenta color. This reaction can help pathologists identify and diagnose various medical conditions, such as cancer, infection, and inflammation.

Mucins are high molecular weight, heavily glycosylated proteins that are the major components of mucus. They are produced and secreted by specialized epithelial cells in various organs, including the respiratory, gastrointestinal, and urogenital tracts, as well as the eyes and ears.

Mucins have a characteristic structure consisting of a protein backbone with numerous attached oligosaccharide side chains, which give them their gel-forming properties and provide a protective barrier against pathogens, environmental insults, and digestive enzymes. They also play important roles in lubrication, hydration, and cell signaling.

Mucins can be classified into two main groups based on their structure and function: secreted mucins and membrane-bound mucins. Secreted mucins are released from cells and form a physical barrier on the surface of mucosal tissues, while membrane-bound mucins are integrated into the cell membrane and participate in cell adhesion and signaling processes.

Abnormalities in mucin production or function have been implicated in various diseases, including chronic inflammation, cancer, and cystic fibrosis.

Mucus is a viscous, slippery secretion produced by the mucous membranes that line various body cavities such as the respiratory and gastrointestinal tracts. It serves to lubricate and protect these surfaces from damage, infection, and foreign particles. Mucus contains water, proteins, salts, and other substances, including antibodies, enzymes, and glycoproteins called mucins that give it its characteristic gel-like consistency.

In the respiratory system, mucus traps inhaled particles such as dust, allergens, and pathogens, preventing them from reaching the lungs. The cilia, tiny hair-like structures lining the airways, move the mucus upward toward the throat, where it can be swallowed or expelled through coughing or sneezing. In the gastrointestinal tract, mucus helps protect the lining of the stomach and intestines from digestive enzymes and other harmful substances.

Excessive production of mucus can occur in various medical conditions such as allergies, respiratory infections, chronic lung diseases, and gastrointestinal disorders, leading to symptoms such as coughing, wheezing, nasal congestion, and diarrhea.

'Staining and labeling' are techniques commonly used in pathology, histology, cytology, and molecular biology to highlight or identify specific components or structures within tissues, cells, or molecules. These methods enable researchers and medical professionals to visualize and analyze the distribution, localization, and interaction of biological entities, contributing to a better understanding of diseases, cellular processes, and potential therapeutic targets.

Medical definitions for 'staining' and 'labeling' are as follows:

1. Staining: A process that involves applying dyes or stains to tissues, cells, or molecules to enhance their contrast and reveal specific structures or components. Stains can be categorized into basic stains (which highlight acidic structures) and acidic stains (which highlight basic structures). Common staining techniques include Hematoxylin and Eosin (H&E), which differentiates cell nuclei from the surrounding cytoplasm and extracellular matrix; special stains, such as PAS (Periodic Acid-Schiff) for carbohydrates or Masson's trichrome for collagen fibers; and immunostains, which use antibodies to target specific proteins.
2. Labeling: A process that involves attaching a detectable marker or tag to a molecule of interest, allowing its identification, quantification, or tracking within a biological system. Labels can be direct, where the marker is directly conjugated to the targeting molecule, or indirect, where an intermediate linker molecule is used to attach the label to the target. Common labeling techniques include fluorescent labels (such as FITC, TRITC, or Alexa Fluor), enzymatic labels (such as horseradish peroxidase or alkaline phosphatase), and radioactive labels (such as ³²P or ¹⁴C). Labeling is often used in conjunction with staining techniques to enhance the specificity and sensitivity of detection.

Together, staining and labeling provide valuable tools for medical research, diagnostics, and therapeutic development, offering insights into cellular and molecular processes that underlie health and disease.

Electrophoresis, polyacrylamide gel (EPG) is a laboratory technique used to separate and analyze complex mixtures of proteins or nucleic acids (DNA or RNA) based on their size and electrical charge. This technique utilizes a matrix made of cross-linked polyacrylamide, a type of gel, which provides a stable and uniform environment for the separation of molecules.

In this process:

1. The polyacrylamide gel is prepared by mixing acrylamide monomers with a cross-linking agent (bis-acrylamide) and a catalyst (ammonium persulfate) in the presence of a buffer solution.
2. The gel is then poured into a mold and allowed to polymerize, forming a solid matrix with uniform pore sizes that depend on the concentration of acrylamide used. Higher concentrations result in smaller pores, providing better resolution for separating smaller molecules.
3. Once the gel has set, it is placed in an electrophoresis apparatus containing a buffer solution. Samples containing the mixture of proteins or nucleic acids are loaded into wells on the top of the gel.
4. An electric field is applied across the gel, causing the negatively charged molecules to migrate towards the positive electrode (anode) while positively charged molecules move toward the negative electrode (cathode). The rate of migration depends on the size, charge, and shape of the molecules.
5. Smaller molecules move faster through the gel matrix and will migrate farther from the origin compared to larger molecules, resulting in separation based on size. Proteins and nucleic acids can be selectively stained after electrophoresis to visualize the separated bands.

EPG is widely used in various research fields, including molecular biology, genetics, proteomics, and forensic science, for applications such as protein characterization, DNA fragment analysis, cloning, mutation detection, and quality control of nucleic acid or protein samples.

Carbohydrates are a major nutrient class consisting of organic compounds that primarily contain carbon, hydrogen, and oxygen atoms. They are classified as saccharides, which include monosaccharides (simple sugars), disaccharides (double sugars), oligosaccharides (short-chain sugars), and polysaccharides (complex carbohydrates).

Monosaccharides, such as glucose, fructose, and galactose, are the simplest form of carbohydrates. They consist of a single sugar molecule that cannot be broken down further by hydrolysis. Disaccharides, like sucrose (table sugar), lactose (milk sugar), and maltose (malt sugar), are formed from two monosaccharide units joined together.

Oligosaccharides contain a small number of monosaccharide units, typically less than 20, while polysaccharides consist of long chains of hundreds to thousands of monosaccharide units. Polysaccharides can be further classified into starch (found in plants), glycogen (found in animals), and non-starchy polysaccharides like cellulose, chitin, and pectin.

Carbohydrates play a crucial role in providing energy to the body, with glucose being the primary source of energy for most cells. They also serve as structural components in plants (cellulose) and animals (chitin), participate in various metabolic processes, and contribute to the taste, texture, and preservation of foods.

... periodic acid-schiff reaction MeSH E05.200.500.620.670.660 - prussian blue reaction MeSH E05.200.500.620.670.770 - shadowing ( ... periodic acid-schiff reaction MeSH E05.200.750.600.670.660 - prussian blue reaction MeSH E05.200.750.600.670.770 - shadowing ( ... periodic acid-schiff reaction MeSH E05.200.500.607.810 - prussian blue reaction MeSH E05.200.500.620 - histocytological ... periodic acid-schiff reaction MeSH E05.200.750.551.810 - prussian blue reaction MeSH E05.200.750.600 - histocytological ...
The reaction of periodic acid oxidizes the vicinal diols in these sugars, usually breaking up the bond between two adjacent ... Alcian blue/periodic acid-Schiff (AB/PAS or AB-PAS) uses alcian blue before the PAS step. PAS staining is mainly used for ... Periodic acid-Schiff (PAS) is a staining method used to detect polysaccharides such as glycogen, and mucosubstances such as ... These aldehydes then react with the Schiff reagent to give a purple-magenta color. A suitable basic stain is often used as a ...
According to a 2015 study, the cost in the United States of testing with the periodic acid-Schiff stain (PAS) was about $148. ... In some cases, WSO is a misdiagnosis of "keratins granulations" which are not a fungus, but a reaction to nail polish that can ... Nail plate biopsy with periodic acid-Schiff stain appear more useful than culture or direct KOH examination. To reliably ... Dermatophytids can be thought of as an allergic reaction to the fungus. The causative pathogens of onychomycosis are all in the ...
Schiff-type reagents are used for various biological tissue staining methods, e.g. Feulgen stain and periodic acid-Schiff stain ... The Schiff test is an early organic chemistry named reaction developed by Hugo Schiff, and is a relatively general chemical ... The further reaction of the Schiff reagent with aldehydes is complex with several research groups reporting multiple reaction ... "The structure of Schiff reagent aldehyde adducts and the mechanism of the Schiff reaction as determined by nuclear magnetic ...
Periodic Acid Schiff (PAS) staining will reveal similar broad hyphae in the dermis and cutis. Fungal culture can also confirm ... Quantitative polymerase chain reaction to detect serum DNA of the pathogen shows promise. The condition may affect the ...
Earlier studies had reported that these cells express vimentin, periodic acid-Schiff, CD34, CD68, and S100 proteins in many ... inflammatory infiltrates which obscure other cell types and thereby suggest that the lesion is a purely inflammatory reaction. ... readily stained with acid dyes) nucleoli. These cells have been termed Reed-Sternberg cell-like, virocyte-like (i.e. cells ...
... is also used in conjunction with periodic acid-Schiff stain in histology. For example, glycogen is darkly stained by ... Silverman, Richard B. (2002). The Organic Chemistry of Enzyme-catalyzed Reactions (2nd ed.). London: Academic Press. p. 1. ISBN ... the principal products of its reactions, and their applications to the industrial arts), Annales de chimie et de physique, 2nd ...
Several staining agents use in microscopy are based around periodate (e.g. periodic acid-Schiff stain and Jones' stain). ... Cleavage reactions proceed via a cyclic intermediate called a periodate ester. The formation of this may be affected by pH and ... 820 Under strongly acid conditions periodic acid can be protonated to give the orthoperiodonium cation. H 6 IO 6 + ↽ − − ⇀ H 5 ... α-keto acids, α-hydroxy acids, amino acids, 1,2-amino alcohols, and 1,2-diamines, to give aldehydes, ketones, and carboxylic ...
... periodic acid-Schiff stain, or an immunofluorescence assay shows the characteristic cysts. The cysts resemble crushed ping-pong ... and more recently by molecular analysis of polymerase chain reaction products comparing DNA samples. Notably, simple molecular ...
... see immunofluorescense of skin tissues Generally 3 sections for H&E staining and one section with periodic acid Schiff (PAS) If ... If negative, an unspecific lymphohistocytic dermatosis may be caused by drug reactions and viral infections. Foreign bodies ... "Skin inflammatory (nontumor) > Lichenoid and interface reaction patterns > Lupus: systemic lupus erythematosus (SLE)". ... "Skin inflammatory (nontumor) > Spongiotic, psoriasiform and pustular reaction patterns > Seborrheic dermatitis". ...
Periodic acid-Schiff stained slide, and Gomori's methenamine silver stained slide), mycological culture and modern molecular ... This honeycomb is a painful inflammatory reaction with deep suppurative lesions on the scalp. Follicles may be seen discharging ...
It is also used to stain cartilage in bone specimens, as one of Kasten's Schiff-type reagents in the periodic acid-Schiff stain ... because the diamine serves both as a source of the diazonium cation and as the coupling partner in the azo coupling reaction. ... Bismarck brown Y stains acid mucins to yellow color. It also stains mast cell granules brown. It can be used with live cells. ... It can also be used as a counterstain for Victoria blue R for staining of acid-fast microorganisms. Lillie, Ralph Dougall (1977 ...
Jones' stain, a methenamine silver-Periodic acid-Schiff that stains for basement membrane, availing to view the "spiked" ... It is used in the Duff reaction (formylation of arenes), the Sommelet reaction (converting benzyl halides to aldehydes), and in ... As the mandelic acid salt (methenamine mandelate) or the hippuric acid salt (methenamine hippurate), it is used for the ... However, it was only used in cases of acidic urine, whereas boric acid was used to treat urinary tract infections with alkaline ...
Periodic acid-Schiff (PAS) stains can be used to observe the thick-walled adiaspores in tissue regions. Sequencing can be used ... It is termed an infection, but better described as a bodily reaction to foreign material, invoking various cellular processes ... At this site the spores become calcified which causes a slight localized reaction involving inflammation. Lung function may be ...
Due to the high volume of carbohydrates within the cell wall of hyphae and yeast forms of fungi, the Periodic acid -Schiff ... This method was discovered by Italian Camillo Golgi, by using a reaction between silver nitrate and potassium dichromate, thus ... "Periodic Acid-Schiff (PAS): Diagnostic Applications - LabCE.com, Laboratory Continuing Education". labce.com. Retrieved 2020-04 ... Periodic acid-Schiff is a histology special stain used to mark carbohydrates (glycogen, glycoprotein, proteoglycans). PAS is ...
... reagent Luche reduction Maillard reaction Madelung synthesis Malaprade reaction, Periodic acid oxidation Malonic ester ... isatin synthesis Sandmeyer reaction Sanger reagent Saponification Sarett oxidation Schiemann reaction Schiff reaction Schiff ... reaction Ponzio reaction Prato reaction Prelog strain Prevost reaction Prileschajew reaction Prilezhaev reaction Prins reaction ... Beckwith ring expansion reaction Duff reaction Dutt-Wormall reaction Dyotropic reaction E1cB elimination reaction Eder reaction ...
... such as periodic acid schiff and Gömöri methenamine silver, can be used to demonstrate the fungal organisms if needed.[citation ... "Rapid identification of Fonsecaea by duplex polymerase chain reaction in isolates from patients with chromoblastomycosis". ...
Luxol fast blue/periodic acid-Schiff, Luxol fast blue/hematoxylin/eosin, or immunohistochemistry for myelin proteins) ... The reactions have been diverse according to the sources of the disease but pathological confirmed MS (damage fulfills all ... Some interactions between the Epstein-Barr virus and the HERVs could be the trigger of the MS microglia reactions. Supporting ... Though the article points to heterogeneous metabolic reactions to interferons instead of disease heterogeneity, it has been ...
Histologic analysis of liver tissue demonstrates moderately decreased amounts of periodic acid-Schiff (PAS)-positive, diastase- ... Glycogen synthetase catalyzes the rate-limiting reaction for glycogen synthesis in the liver by transferring glucose units from ... Mutations in the liver isoform (GSY2), causes fasting hypoglycemia, high blood ketones, increased free fatty acids and low ... Plasma amino-acid analysis shows plasma alanine levels as in reference ranges during a fast. Skeletal radiography may reveal ...
This reaction between 249Bk and 50Ti was predicted to be the most favorable practical reaction for formation of element 119, as ... Schiff, L. I.; Snyder, H.; Weinberg, J. (1940). "On the Existence of Stationary States of the Mesotron Field". Physical Review ... Unseptbium is expected to be a strong Lewis acid, forming fluorides and oxides, similarly to its lighter congener xenon. ... An extended periodic table theorises about chemical elements beyond those currently known in the periodic table and proven. The ...
After separation of red cell membranes by SDS-polyacrylamide gel electrophoresis and staining with periodic acid-Schiff ... Antibodies to the Gerbich antigens have been associated with transfusion reactions and mild hemolytic disease of the newborn. ... Glycophorin C (GPC) is a single polypeptide chain of 128 amino acids and is encoded by a gene on the long arm of chromosome 2 ( ... These exons also differ by a 9 amino acid insert at the 3' end of exon 3. The direct repeated segments containing these exons ...
Smears and biopsies are usually stained with periodic acid-Schiff, which stains carbohydrates in fungal cell walls in magenta. ... and is the site of cell mediated immune reactions. Competition and inhibition interactions between candida species and other ... An alternative method of disinfection is to use a 10% solution of acetic acid (vinegar) as an overnight soak, or to microwave ... Malnutrition, whether by malabsorption, or poor diet, especially hematinic deficiencies (iron, vitamin B12, folic acid) can ...
Schiff 2011, p. 1 John von Neumann, "The general and logical theory of automata," in L.A. Jeffress, ed., Cerebral Mechanisms in ... The Belousov-Zhabotinsky reaction is a spatio-temporal chemical oscillator that can be simulated by means of a cellular ... More generally, it is sometimes assumed that the universe starts out covered with a periodic pattern, and only a finite number ... homogenous layer of a mixture of malonic acid, acidified bromate, and a ceric salt were mixed together and left undisturbed, ...
Interest arose in the 1960s when several authors drew attention to the presence of periodic-acid-Schiff-positive (PAS-positive ... this suggests that PNNs are either instrumental in their formation or are a reaction to their formation. In vitro studies have ...
This periodic turning also allows contaminants present in the soil to be uniformly distributed which accelerates the process of ... Mora RH, Macbeth TW, MacHarg T, Gundarlahalli J, Holbrook H, Schiff P (2008). "Enhanced bioremediation using whey powder for a ... The added organic substrates are first fermented to hydrogen (H2) and volatile fatty acids (VFAs). The VFAs, including acetate ... Most bioremediation processes involve oxidation-reduction reactions where either an electron acceptor (commonly oxygen) is ...
2 abgebildet." (An illustration of the crystalline acid, see Fig. 1 ; the sodium salt of conium acid is depicted in Fig. 2.) ... Note: although the scheme below shows a single enantiomer of coniine, the final reaction produces a racemic mixture that is ... Hemlock poisoning has been a periodic human concern, a regular veterinary concern, and has had significant occurrences in human ... a Schiff base differing from coniine only by its carbon-nitrogen double bond in the ring. This pathway results in natural ...
... is a white compound that can be made by dissolving radium carbonate in nitric acid. As the concentration of nitric acid ... It is the sixth element in group 2 of the periodic table, also known as the alkaline earth metals. Pure radium is silvery-white ... For this reason, the standard electrode potential for the half-reaction Ra2+ (aq) + 2e− → Ra (s) is −2.916 V, even slightly ... "Nuclear Time-Reversal Violation and the Schiff Moment of $^{225}\mathrm{Ra}$". Physical Review Letters. 94 (23): 232502. arXiv: ...
First, hydrochloric acid, or a mixture of dilute sulfuric acid and sodium chloride was made to react with manganese dioxide, ... The OEC is responsible for the terminal photooxidation of water during the light reactions of photosynthesis, i.e., it is the ... Bearing Schiff Base and Simple Anionic Ligands". Accounts of Chemical Research. 47 (2): 427-439. doi:10.1021/ar400147y. PMID ... Manganese and compounds Fact Sheet International Manganese Institute NIOSH Manganese Topic Page Manganese at The Periodic Table ...
He discovered a number of comets, including periodic comets 54P/de Vico-Swift-NEAT and 122P/de Vico Giambattista della Porta (c ... Acetylsalicylic acid. Wiley-VCH, 2009. p. 6. Web. 3 March 2011. "Bernardino Ramazzini" Encyclopædia Britannica Online, 2011. ... she is regarded as one of the most prominent European figures in fashion between the two World Wars Michela Schiff Giorgini ( ... discoverer of the Paternò-Büchi reaction (1909) Giuseppe Peano (1858-1932), mathematician and a founder of symbolic logic whose ...
"Periodic Acid-Schiff Reaction" by people in this website by year, and whether "Periodic Acid-Schiff Reaction" was a major or ... Periodic Acid-Schiff Reaction*Periodic Acid-Schiff Reaction. *Periodic Acid Schiff Reaction ... "Periodic Acid-Schiff Reaction" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH ( ... Below are the most recent publications written about "Periodic Acid-Schiff Reaction" by people in Profiles. ...
Periodic Acid-Schiff Reaction. Hyaline globules in neuroendocrine and solid-pseudopapillary neoplasms of the pancreas: a clue ...
... routinely diagnosed in biopsies from the small intestine and other tissues by periodic acid-Schiff (PAS) diastase staining and ... routinely diagnosed in biopsies from the small intestine and other tissues by periodic acid-Schiff (PAS)-diastase staining and ... periodic acid-Schiff reaction; qPCR, quantitative polymerase chain reaction; (r)RNA, (ribosomal) ribonucleic acid; T. whipplei ... In 1949, intra-mucosal macrophages with a granular, periodic acid-Schiff (PAS) reaction positive cytoplasm in the small ...
Categories: Periodic Acid-Schiff Reaction Image Types: Photo, Illustrations, Video, Color, Black&White, PublicDomain, ...
... or with the periodic acid - Schiff (PAS) reaction. No histological difference was observed in fruit treated with 2% CaCl2. ...
... acid-Schiff reaction ( PAS ) the Schiff reagent is a bleached basic that... Periodic acid-Schiff reaction ( PAS ) the Schiff ... Periodic acid-Schiff reaction (PAS) The Schiff reagent is a bleached basic fuschin that reacts with aldehyde groups. . This ... This reaction results in a deep red colour in the section. In this study, shared neurobiologic processes were associated with ... Histology Rules to code the most specific histologic term or sand paper ) the Schiff is. Slides, mnemonics, histology games, ...
... periodic acid-schiff reaction MeSH E05.200.500.620.670.660 - prussian blue reaction MeSH E05.200.500.620.670.770 - shadowing ( ... periodic acid-schiff reaction MeSH E05.200.750.600.670.660 - prussian blue reaction MeSH E05.200.750.600.670.770 - shadowing ( ... periodic acid-schiff reaction MeSH E05.200.500.607.810 - prussian blue reaction MeSH E05.200.500.620 - histocytological ... periodic acid-schiff reaction MeSH E05.200.750.551.810 - prussian blue reaction MeSH E05.200.750.600 - histocytological ...
Periodic acid Schiff reaction in childhood lymphoblastic leukaemia. The Medical Research Council Working Party on Childhood ... 2009). Studies on Periodic Acid - Schiff Reactive Material in White Blood Cells from the Peripheral Blood of Patients with ... Sharvill, D. (1952). The Periodic Acid-Schiff Stain in the Diagnosis of Dermatomycoses. British Journal of Dermatology, 64(9), ... Glycogen concentrations were measured through periodic acid Schiffs staining (PAS) method and by using an enzymatic detection ...
A positive reaction was observed, when AF samples were stained. with periodic acid-Schiff and the sialic acid-specific Sambucus ... nitrate and the second part for glycoproteins with periodic acid-Schiff. reagent (Glycoprotein Staining Kit; Pierce) according ... sialic acid-specific Sambus Nigra lectin (DIG Glycan Differentiation Kit;. Boehringer Mannheim). Nonspecific binding was blocked ... enrichment and multiple reaction monitoring mass spectrometry-based quanti-. fication of protein biomarkers. Mol. Cell. ...
... histopathology with periodic acid-Schiff staining, fungal culture, and polymerase chain reaction) is important; however, more ... These treatments can vary from various types of oral medications, acids, ablative modalities, and injections. In this article, ...
Periodic acid-Schiff and mucicarmine stains showed negative reaction. Immunohistochemical study using antibody for cytokeratin ... The neoplastic cells showed complete negative reaction with antibodies for vimentin, smooth muscle actin and homatropine ...
Periodic Acid-Schiff Reaction (PAS): highlights secretions, basement membranes, and microvilli. * Glycogen stains deep red or ... peroxisomes are membrane-bounded organelles housing oxidative enzymes such as urate oxidase, D-amino acid oxidase, and catalase ... they also function in detoxification of certain toxins and in elongation of some fatty acids during lipid synthesis ... energy-generating organelles that contain the enzymes of the citric acid cycle, the respiratory chain, and oxidative ...
Cytoplasmic inclusions did not stain with the periodic acid-Schiff (PAS) reaction. Using a rabbit polyclonal antibody against ... The major fatty acids were C16â :â 0, C18â :â 1 and C18â :â 0. Biochemical and phylogenetic analyses clearly distinguished the ...
B) Periodic acid Schiff stain. Segmented neutrophil and blast cells stain positive with red reaction product. Note the ... Periodic acid Schiff stain. Leukemic erythroblasts characteristically react with this stain with a reddish colored reaction ... Periodic acid Schiff stain displaying intensely positive reaction (red granular cytoplasmic staining) characteristic of ... B) Periodic acid Schiff stain. Note intensely stained leukemic erythroblasts (red granular staining of cytoplasm). Giant ...
WD Whipples disease, PAS periodic acid-Schiff, PCR polymerase chain reaction, TW IHC T. whipplei-specific immunohistochemistry ... usually based on periodic acid-Schiff (PAS) staining of macrophages in the duodenal lamina propria [2] and should be confirmed ... Reliable diagnosis based on a combination of periodic acid-Schiff (PAS) staining, T. whipplei-specific immunohistochemistry ( ... PAS periodic acid-Schiff, TW T. whipplei , EDTA EDTA-blood, IHC immunohistochemistry , PBMC peripheral blood mononuclear cells ...
... periodic acid-Schiff; PCR: polymerase chain reaction; p-Smad3: phospho-Smad3; p-IκBα: phospho-IkBα; p-NF-kB/65: phospho-NF-kB/ ... cat# sc-1351), IL-1β (Abcam, cat# 205924) and MCP-1 (Santa Cruz Biotechnology, cat# sc-1785). The Periodic Acid-Schiff (PAS) ... Nucleic Acids Res. 2007;35:890-901 Author contact. Corresponding authors: Professor Cheng Wang, Division of Nephrology, ... findings that the early prediabetic but not the diabetic treatment on db/db mice with the combined Smad7 agonist Asiatic acid ...
... positive reactions and lipid granules in cytoplasm of the Leydig cells in the CP group increased compared with the other groups ... Periodic acid-schiff (PAS) reaction in the A. Control (C) group, B. Cyclophosphamide (CP) and C. CP+ethyl pyruvate (EP) groups ... periodic acid-schiff (PAS) positive reactions and lipid granules in cytoplasm of the Leydig cells in the CP group increased ... Granules that contain carbohydrate compounds were stained with periodic acid-schiff (PAS) (17). PAS positive granules stained a ...
Bone marrow film (1000× magnification) demonstrating granular and clotlike positive reaction in periodic acid-Schiff staining ...
The sections were cut at 5 µm thickness and stained by the periodic acid-Schiff (PAS) reaction, after short stays in degrading ... The bicinchoninic acid method was used for determining the amount of protein in samples.[27] Lipid peroxidation determination. ... Immunohistochemical reactions were performed according to the ABC technique described by Hsu et al.[32] The procedure involved ... Then, they were cut into small fragments and fixed in gender fluid (picric acid saturated solution in 90% ethanol; 40% ...
Histopathologic examination with a periodic acid-Schiff stain. Histopathologic examination of nail clippings with a periodic ... Polymerase chain reaction. Polymerase chain reaction (PCR) is a quick and increasingly used method for diagnosing ringworm. ... acid-Schiff (PAS) stain is a method for confirming the diagnosis for patients with suspected onychomycosis, a fungal nail ...
Tests can include cytology smears; biopsy and staining with periodic acid-Schiff (PAS) or Gomori methenamine silver stain; ... Oral geotrichosis presents as pseudomembranous plaques with an underlying chronic inflammatory reaction, which can resemble ... Investigations include smears, biopsy, staining with periodic acid-Schiff (PAS) or Gomori methenamine silver, culture of the ... affected tissues, polymerase chain reaction (PCR), serodiagnosis (sometimes), physical examination, and chest radiography. ...
Periodic Acid-Schiff Reaction, Prognosis, Radiotherapy, Risk Assessment, Secondary Prevention, Treatment Outcome ...
Periodic Acid-Schiff Reaction E1.370.225.500.620.670.615 Periodontal Index E5.318.308.250.300.725 E5.318.308.980.438.300.725 ... Arachidonic Acid D10.251.355.96.100 Arachidonic Acids D10.251.355.96 Arachis hypogaea J2.500.850.500.77 (Replaced for 2015 by ... Fusidic Acid D10.570.938.515 Fuzzy Logic L1.224.65.250 L1.224.50.375.250 G-Quadruplexes G2.111.570.790.486.550 G2.111.570.820. ... 13-dienoic Acid D2.737.637.650.500.500 D1.248.497.158.685.750.744.650.500.500 D10.251.355.255.100.637.25.650.500.500 D1.339. ...
Periodic Acid-Schiff Reaction E1.370.225.500.620.670.615 Periodontal Index E5.318.308.250.300.725 E5.318.308.980.438.300.725 ... Arachidonic Acid D10.251.355.96.100 Arachidonic Acids D10.251.355.96 Arachis hypogaea J2.500.850.500.77 (Replaced for 2015 by ... Fusidic Acid D10.570.938.515 Fuzzy Logic L1.224.65.250 L1.224.50.375.250 G-Quadruplexes G2.111.570.790.486.550 G2.111.570.820. ... 13-dienoic Acid D2.737.637.650.500.500 D1.248.497.158.685.750.744.650.500.500 D10.251.355.255.100.637.25.650.500.500 D1.339. ...
Periodic Acid-Schiff Reaction E1.370.225.500.620.670.615 Periodontal Index E5.318.308.250.300.725 E5.318.308.980.438.300.725 ... Arachidonic Acid D10.251.355.96.100 Arachidonic Acids D10.251.355.96 Arachis hypogaea J2.500.850.500.77 (Replaced for 2015 by ... Fusidic Acid D10.570.938.515 Fuzzy Logic L1.224.65.250 L1.224.50.375.250 G-Quadruplexes G2.111.570.790.486.550 G2.111.570.820. ... 13-dienoic Acid D2.737.637.650.500.500 D1.248.497.158.685.750.744.650.500.500 D10.251.355.255.100.637.25.650.500.500 D1.339. ...
N2.421.143.620.550 Periodic Acid-Schiff Reaction E5.200.750.600.670.620 E1.370.225.500.607.790 E1.370.225.750.551.790 ... D1.632.825.375 Technetium Tc 99m Diethyl-iminodiacetic Acid D1.632.825.445 Technetium Tc 99m Dimercaptosuccinic Acid D1.632. ... E2.520.89 Arsanilic Acid D1.632.88.30 Arsenamide D1.632.88.40 Arsenates D1.632.88.42 Arsenazo III D1.632.88.44 Arsenicals ... D12.644.548.586.200.500.250 Cacodylic Acid D1.632.88.100 CADASIL C10.228.140.300.275.249 C14.907.253.329.249 Candicidin D4.345. ...
  • Cytoplasmic inclusions did not stain with the periodic acid-Schiff (PAS) reaction. (bvsalud.org)
  • Note metachromatic staining (reddish to purple color reaction with a blue stain). (oncohemakey.com)
  • Periodic acid Schiff stain. (oncohemakey.com)
  • Leukemic erythroblasts characteristically react with this stain with a reddish colored reaction product. (oncohemakey.com)
  • Histopathologic examination of nail clippings with a periodic acid-Schiff (PAS) stain is a method for confirming the diagnosis for patients with suspected onychomycosis , a fungal nail infection most often caused by dermatophytes. (cdc.gov)
  • To determine the properties of the secretions of the ceruminous glands in the meatus acusticus externus of cats and dogs, the periodic acid Schiff (PAS) stain was used for neutral mucosubstances and glycogen, while the Alcian blue stain (pH 2.5) was applied for the presence of acidic mucosubstances. (tubitak.gov.tr)
  • The Alcian blue stain (pH 2.5) for acidic mucosubstances gave positive reactions for cats and negative reactions for dogs. (tubitak.gov.tr)
  • The organisms are gram negative, and highlighted with Giemsa and Macchiavello stains, but do not stain with periodic acid-Schiff. (vin.com)
  • Periodic Acid Schiff (PAS) stain highlights(fuchsia) the vascular walls and (asterix line) and the branching fungus invading(arrow heads) the vascular lumen(L). Diffuse necrosis and poor inflammatory reaction in the background with destruction of normal anatomy. (eyewiki.org)
  • The resulting aldehydes react with Schiff reagent to form a colored product. (umassmed.edu)
  • Periodic acid-Schiff reaction (PAS) The Schiff reagent is a bleached basic fuschin that reacts with aldehyde groups. (feministlawprofessors.com)
  • The reaction of periodic acid selectively oxidizes the glucose residues, creates aldehydes that react with the Schiff reagent and creates a purple-magenta color. (wikidoc.org)
  • Periodic Acid/Schiff Reagent (PAS) Staining. (edrc2013.org)
  • Reliable diagnosis based on a combination of periodic acid-Schiff (PAS) staining, T. whipplei-specific immunohistochemistry (IHC), and polymerase chain reaction (PCR) from duode-nal biopsies may be challenging in cases without classical gastrointestinal infection, so the need for additional diag-nostic materials is urgent. (123dok.net)
  • RpoB gene polymerase chain reaction (PCR) can be an additional tool to assist in the diagnosis of Whipple's disease. (123dok.net)
  • Polymerase chain reaction (PCR) is a quick and increasingly used method for diagnosing ringworm. (cdc.gov)
  • Investigations include smears, biopsy, staining with periodic acid-Schiff (PAS) or Gomori methenamine silver, culture of the affected tissues, polymerase chain reaction (PCR), serodiagnosis (sometimes), physical examination, and chest radiography. (medscape.com)
  • Polymerase chain reactions (PCR) and Sanger sequencing were performed on samples from one pup. (vin.com)
  • At the end of 8 weeks, all rats were sacrificed to obtain the kidney tissues for histological examination and reverse transcription polymerase chain reaction (RT-PCR) was used to analyze the transcriptional levels of nephrin and podocin genes. (biomedcentral.com)
  • Deoxyribonucleic acid from FFPE tissue samples and control blood samples was amplified by high-resolution melt real-time polymerase chain reaction before sequencing. (bvsalud.org)
  • Whipple's disease (WD) is a rare chronic systemic infection with a wide range of clinical symptoms, routinely diagnosed in biopsies from the small intestine and other tissues by periodic acid-Schiff (PAS) diastase staining and immunohistological analysis with specific antibodies. (frontiersin.org)
  • Glycogen concentrations were measured through periodic acid Schiff's staining (PAS) method and by using an enzymatic detection kit, in various time points. (concordia.ca)
  • immunohistochemistry (IHC) and periodic acid- Schiff (PAS) staining. (123dok.net)
  • In dogs, positive staining was observed for glycogen and neutral mucosubstances, whereas, in cats, negative reactions were observed. (tubitak.gov.tr)
  • Periodic acid-Schiff ( PAS ) is a staining method used in histology and pathology . (wikidoc.org)
  • Periodic acid-Schiff and mucicarmine stains showed negative reaction. (jnsbm.org)
  • Periodic Acid-Schiff, Acid-Fast Bacillus and Gram stains were negative for microorganisms. (juniperpublishers.com)
  • Histologic sections were stained with hematoxylin-eosin, periodic acid-Schiff, and Masson trichrome stains. (cumhuriyet.edu.tr)
  • While some of these are special fungal stains, others are of more general use but still help to observe the tissue reactions and/or the infecting fungus. (drfungus.org)
  • The reactions involved and comments of particular interest for some of these stains are summarized in the TABLE. (drfungus.org)
  • Chemotherapy with CP disrupts reductive reactions in tissues and creates oxidative stress (1,3) as an alkylating agent, finally reducing fertilization in patients under treatment (4,5). (ijfs.ir)
  • The diastase reaction was applied to confirm the presence of glycogen. (tubitak.gov.tr)
  • The positive reactions observed in dogs were found not to originate from glycogen by the negative diastase reactions observed. (tubitak.gov.tr)
  • Gomori methenamine silver (GMS), Gridley's fungus (GF), and periodic acid-Schiff (PAS) are special for and very efficient to visualize the fungi. (drfungus.org)
  • a positive periodic acid-Schiff (PAS) reaction in macro- phages from a small bowel biopsy sample ( 2 ). (cdc.gov)
  • Excision biopsy of deep cut sections of the lesion revealed sulfur granules with a positive periodic acid Schiff reaction and Mycetoma is caused by fungi or fungus- eumycotic mycetoma was suggested. (who.int)
  • Calcium oxalate crystals, probably originating from fungal oxalic acid, are present near hyphae in necrotic tissue, if the infecting species is Aspergillus niger . (drfungus.org)
  • A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes. (uams.edu)
  • After 4 hr, the reaction was stopped with MeOH/1 N HCl (1:1), and the lipid was extracted with chloroform. (edrc2013.org)
  • Histologic sections did not reveal inflammatory reaction, cell degeneration, and cell necrosis. (cumhuriyet.edu.tr)
  • The mechanisms by which isocyanates cause asthma remain unclear, hampering disease prevention, diagnosis, and treatment.7 It is assumed the reactive nature of N=C=O groups underlies isocyanate asthma, with a hapten-based mechanism as the most obvious pathway to pathogenesis.8 However, the critical "self" reaction targets for isocyanate in vivo remain uncertain. (cdc.gov)
  • Positive reaction (black reaction product) in myeloblasts and immature eosinophils. (oncohemakey.com)
  • The major advantages of histopathology are speed, low-cost, and the ability to provide a presumptive identification of the infecting fungus, as well as demonstrating the tissue reaction. (drfungus.org)
  • Thus, a combination of GMS and H&E is usually employed to visualize both the tissue reaction and the infecting fungus. (drfungus.org)
  • The neoplastic cells showed complete negative reaction with antibodies for vimentin, smooth muscle actin and homatropine bromide-45 antigens. (jnsbm.org)
  • 3, 4 Several tumour vaccines directed against MUC1 core protein, oligosaccharides, or MUC1 cDNAs have been shown to stimulate immune reactions in basic clinical trials to confer antitumour activity. (bmj.com)
  • The biopsies of both sites showed findings consistent with psoriasiform dermatitis and were both periodic acid-Schiff (PAS) negative ( Figure ). (consultant360.com)
  • The cultured cells on a glass slide were reacted with PAS Rifamdin after periodic treatment. (edrc2013.org)
  • This graph shows the total number of publications written about "Periodic Acid-Schiff Reaction" by people in this website by year, and whether "Periodic Acid-Schiff Reaction" was a major or minor topic of these publications. (umassmed.edu)
  • The cereal market shows a more elastic reaction to price responses, even though demand for staple foods is generally inelastic. (bvsalud.org)
  • The paper presents the reaction of the Romanian cereal market to the disruption of trade flows caused by certain shocks, such as the COVID-19 pandemic, which lead to changes with high impact on the functioning of this market, representing an important test for the resilience of the sector. (bvsalud.org)
  • Paraffin sections were stained with either an aqueous mixture of alcian blue 8GX, safaranin 0 and Bismark brown Y, or with the periodic acid - Schiff (PAS) reaction. (usda.gov)
  • Periodic acid-Schiff reaction and prognosis in lymphoblastic leukaemia. (bmj.com)
  • It appears that the PAS reaction can identify long survivors among patients with ALL, but not in the absence of features strongly associated with a poor prognosis. (bmj.com)
  • Whipple's disease may be diagnosed by periodic acid-Schiff (PAS) staining, electron microscopy, or polymerase chain reaction of intestinal biopsy specimens. (nih.gov)
  • Onychomycosis infections: Do polymerase chain reaction and culture reports agree? (msdmanuals.com)
  • Hematoxylin and Eosin, periodic acid-Schiff, inducible nitric oxide synthase, proliferating cell nuclear antigen expression and electron microscopy investigation were performed. (ijpsonline.com)
  • These rods contain the Z-line protein, α -actinin, that can be effectively stained in skeletal muscles using Gomori or Masson trichrome and negatively stained with periodic acid-Schiff. (scielo.org.za)
  • This sweetener is a dipeptide derived from the combination of two non-aromatic amino acids namely, aspartic acids and phenylalanine. (ijfs.ir)
  • After oral intake, aspartame is hydrolyzed in the gastrointestinal tract by esterases and peptidases into amino acids (aspartic acid and phenylalanine) and methanol. (ijfs.ir)
  • Investigators reported that L-carnitine is a compound biosynthesized from the amino acids methionine, and lysine in kidney and liver. (ijpsonline.com)
  • OBJECTIVES: Lichenoid granulomatous reaction can be subcategorized into lichenoid granulomatous dermatitis or lichenoid granulomatous stomatitis. (elsevierpure.com)
  • METHODS AND MATERIALS: We retrieved all cases diagnosed with lichenoid reaction with granulomatous inflammation between January 1, 2000, and August 1, 2016, from the University of Florida Oral Pathology Biopsy Service Archives. (elsevierpure.com)
  • 7. Extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue with plasma cell differentiation: Periodic acid-schiff reaction-positive Dutcher body is a diagnostic clue to distinguish it from plasmacytoma. (nih.gov)
  • Excision biopsy of deep cut sections of the lesion revealed sulfur granules with a positive periodic acid Schiff reaction and Mycetoma is caused by fungi or fungus- eumycotic mycetoma was suggested. (who.int)
  • The neutrophilic granulocytes (NG) and monocytes functional activity, the content of myeloperoxidase and substances with a positive reaction to Schiff's periodic acid (PAS- stained substances), the cytokines content were determined: interleukin-1β (IL-1β), IL-2, IL-4, IL- 6, tumor necrosis factor-α (TNF-α). (kiev.ua)
  • It is also significant in life science applications as the Periodic Acid-Schiff reaction for the analysis of intracellular polysaccharides like glycogen, starch and cellulose. (williamblythe.com)
  • The periodic acid-Schiff reaction showed the presence of higher glycogen content material (pink) in cells from FCS and LIF conditions but considerably lower levels in the CNTF condition. (dhfrinhibitor.com)
  • For LIF-differentiated astrocytes, glycogen levels remained elevated for two h and decreasedCytologic Reaction for Glycogen and ImmunocytochemistryCytologic localization of glycogen was performed working with the periodic acid-Schiff method (Rosenberg and Dichter, 1985). (dhfrinhibitor.com)
  • Naphthalene toxicity is the result of the biotransformation process, which consists in cellular chemical reactions with the aim of making the compounds more soluble in water, facilitating their excretion. (scielo.br)
  • Aristolochic acids (AAs) are chemical compounds that are also known as nitrophenanthrene carboxylic acid compounds. (biomedcentral.com)
  • The resulting aldehydes react with Schiff reagent to form a colored product. (nih.gov)
  • L-carnitine assists as a cofactor necessary for the transport of long chain fatty acids into the mitochondria for energy production in the peripheral tissues[ 7 ]. (ijpsonline.com)
  • It functions in maintaining acid base balance and constant intraocular temperature [4,5]. (heraldopenaccess.us)
  • There is a significant reduction in nephrotoxicity and infusion-related reactions with continuous 24-h infusion vs conventional 2-h to 6-h infusion. (medscape.com)
  • In this study, we established an aristolochic acid nephropathy (AAN) model by intraperitoneally administering aristolochic acid I sodium salt (AA-I) at a dose of 5 mg/kg body weight on alternate days for 4 weeks. (biomedcentral.com)
  • Our unique production technology uses a proprietary multi-stage process that reduces trace impurities to ppb levels and allows us to produce large volumes of very high purity periodic acid. (williamblythe.com)
  • William Blythe understands that impurity levels are critical to many of the applications of periodic acid and is continually investing to reduce trace impurity levels to meet customer needs. (williamblythe.com)