Pancreas: A nodular organ in the ABDOMEN that contains a mixture of ENDOCRINE GLANDS and EXOCRINE GLANDS. The small endocrine portion consists of the ISLETS OF LANGERHANS secreting a number of hormones into the blood stream. The large exocrine portion (EXOCRINE PANCREAS) is a compound acinar gland that secretes several digestive enzymes into the pancreatic ductal system that empties into the DUODENUM.Endocrine Cells: Secretory cells of the ductless glands. They secrete HORMONES directly into the blood circulation (internal secretion) to be carried to the target cells. The secreted chemicals can be PEPTIDES; STEROIDS; NEUROPEPTIDES; or BIOGENIC AMINES.Islets of Langerhans: Irregular microscopic structures consisting of cords of endocrine cells that are scattered throughout the PANCREAS among the exocrine acini. Each islet is surrounded by connective tissue fibers and penetrated by a network of capillaries. There are four major cell types. The most abundant beta cells (50-80%) secrete INSULIN. Alpha cells (5-20%) secrete GLUCAGON. PP cells (10-35%) secrete PANCREATIC POLYPEPTIDE. Delta cells (~5%) secrete SOMATOSTATIN.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Pore Forming Cytotoxic Proteins: Proteins secreted from an organism which form membrane-spanning pores in target cells to destroy them. This is in contrast to PORINS and MEMBRANE TRANSPORT PROTEINS that function within the synthesizing organism and COMPLEMENT immune proteins. These pore forming cytotoxic proteins are a form of primitive cellular defense which are also found in human LYMPHOCYTES.Endocrine System: The system of glands that release their secretions (hormones) directly into the circulatory system. In addition to the ENDOCRINE GLANDS, included are the CHROMAFFIN SYSTEM and the NEUROSECRETORY SYSTEMS.Insulin-Secreting Cells: A type of pancreatic cell representing about 50-80% of the islet cells. Beta cells secrete INSULIN.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Antigens, CD274: An inhibitory B7 antigen that has specificity for the T-CELL receptor PROGRAMMED CELL DEATH 1 PROTEIN. CD274 antigen provides negative signals that control and inhibit T-cell responses and is found at higher than normal levels on tumor cells, suggesting its potential role in TUMOR IMMUNE EVASION.Programmed Cell Death 1 Receptor: An inhibitory T-lymphocyte receptor that has specificity for CD274 ANTIGEN and PROGRAMMED CELL DEATH 1 LIGAND 2 PROTEIN. Signaling by the receptor limits T cell proliferation and INTERFERON GAMMA synthesis. The receptor also may play an essential role in the regulatory pathway that induces PERIPHERAL TOLERANCE.Antiviral Agents: Agents used in the prophylaxis or therapy of VIRUS DISEASES. Some of the ways they may act include preventing viral replication by inhibiting viral DNA polymerase; binding to specific cell-surface receptors and inhibiting viral penetration or uncoating; inhibiting viral protein synthesis; or blocking late stages of virus assembly.Programmed Cell Death 1 Ligand 2 Protein: A costimulatory B7 antigen that has specificity for the T-CELL receptor PROGRAMMED CELL DEATH 1 RECEPTOR. It is closely-related to CD274 antigen; however, its expression is restricted to DENDRITIC CELLS and activated MACROPHAGES.Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Lupus Erythematosus, Systemic: A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow.Serum Amyloid P-Component: Amyloid P component is a small, non-fibrillar glycoprotein found in normal serum and in all amyloid deposits. It has a pentagonal (pentaxin) structure. It is an acute phase protein, modulates immunologic responses, inhibits ELASTASE, and has been suggested as an indicator of LIVER DISEASE.Autoantibodies: Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.Phagocytosis: The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).Mannose-Binding Lectin: A specific mannose-binding member of the collectin family of lectins. It binds to carbohydrate groups on invading pathogens and plays a key role in the MANNOSE-BINDING LECTIN COMPLEMENT PATHWAY.Antibodies, Antinuclear: Autoantibodies directed against various nuclear antigens including DNA, RNA, histones, acidic nuclear proteins, or complexes of these molecular elements. Antinuclear antibodies are found in systemic autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, scleroderma, polymyositis, and mixed connective tissue disease.Lupus Vasculitis, Central Nervous System: Central nervous system vasculitis that is associated with SYSTEMIC LUPUS ERYTHEMATOSUS. Clinical manifestations may include DEMENTIA; SEIZURES; CRANIAL NERVE DISEASES; HEMIPARESIS; BLINDNESS; DYSPHASIA; and other neurological disorders.Exosomes: A type of extracellular vesicle, containing RNA and proteins, that is secreted into the extracellular space by EXOCYTOSIS when MULTIVESICULAR BODIES fuse with the PLASMA MEMBRANE.Histocompatibility Antigens Class I: Membrane glycoproteins consisting of an alpha subunit and a BETA 2-MICROGLOBULIN beta subunit. In humans, highly polymorphic genes on CHROMOSOME 6 encode the alpha subunits of class I antigens and play an important role in determining the serological specificity of the surface antigen. Class I antigens are found on most nucleated cells and are generally detected by their reactivity with alloantisera. These antigens are recognized during GRAFT REJECTION and restrict cell-mediated lysis of virus-infected cells.Rats, Transgenic: Laboratory rats that have been produced from a genetically manipulated rat EGG or rat EMBRYO, MAMMALIAN. They contain genes from another species.Immunologic Factors: Biologically active substances whose activities affect or play a role in the functioning of the immune system.Antigen Presentation: The process by which antigen is presented to lymphocytes in a form they can recognize. This is performed by antigen presenting cells (APCs). Some antigens require processing before they can be recognized. Antigen processing consists of ingestion and partial digestion of the antigen by the APC, followed by presentation of fragments on the cell surface. (From Rosen et al., Dictionary of Immunology, 1989)Genes, MHC Class I: Genetic loci in the vertebrate major histocompatibility complex which encode polymorphic characteristics not related to immune responsiveness or complement activity, e.g., B loci (chicken), DLA (dog), GPLA (guinea pig), H-2 (mouse), RT-1 (rat), HLA-A, -B, and -C class I genes of man.HLA-B27 Antigen: A specific HLA-B surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-B*27 allele family.Forensic Medicine: The application of medical knowledge to questions of law.Benzodiazepines: A group of two-ring heterocyclic compounds consisting of a benzene ring fused to a diazepine ring.Mass Spectrometry: An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.Tandem Mass Spectrometry: A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.High-Throughput Screening Assays: Rapid methods of measuring the effects of an agent in a biological or chemical assay. The assay usually involves some form of automation or a way to conduct multiple assays at the same time using sample arrays.Factor Analysis, Statistical: A set of statistical methods for analyzing the correlations among several variables in order to estimate the number of fundamental dimensions that underlie the observed data and to describe and measure those dimensions. It is used frequently in the development of scoring systems for rating scales and questionnaires.Chromatography, Liquid: Chromatographic techniques in which the mobile phase is a liquid.Drug Design: The molecular designing of drugs for specific purposes (such as DNA-binding, enzyme inhibition, anti-cancer efficacy, etc.) based on knowledge of molecular properties such as activity of functional groups, molecular geometry, and electronic structure, and also on information cataloged on analogous molecules. Drug design is generally computer-assisted molecular modeling and does not include pharmacokinetics, dosage analysis, or drug administration analysis.Quantitative Structure-Activity Relationship: A quantitative prediction of the biological, ecotoxicological or pharmaceutical activity of a molecule. It is based upon structure and activity information gathered from a series of similar compounds.Informatics: The field of information science concerned with the analysis and dissemination of data through the application of computers.Rhodium: Rhodium. A hard and rare metal of the platinum group, atomic number 45, atomic weight 102.905, symbol Rh. (Dorland, 28th ed)Palladium: A chemical element having an atomic weight of 106.4, atomic number of 46, and the symbol Pd. It is a white, ductile metal resembling platinum, and following it in abundance and importance of applications. It is used in dentistry in the form of gold, silver, and copper alloys.Structure-Activity Relationship: The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.Molecular Structure: The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.Receptors, Purinergic P2Y12: A subclass of purinergic P2Y receptors that have a preference for ADP binding and are coupled to GTP-BINDING PROTEIN ALPHA SUBUNIT, GI. The P2Y12 purinergic receptors are found in PLATELETS where they play an important role regulating PLATELET ACTIVATION.Ticlopidine: An effective inhibitor of platelet aggregation commonly used in the placement of STENTS in CORONARY ARTERIES.Platelet Aggregation Inhibitors: Drugs or agents which antagonize or impair any mechanism leading to blood platelet aggregation, whether during the phases of activation and shape change or following the dense-granule release reaction and stimulation of the prostaglandin-thromboxane system.Platelet Aggregation: The attachment of PLATELETS to one another. This clumping together can be induced by a number of agents (e.g., THROMBIN; COLLAGEN) and is part of the mechanism leading to the formation of a THROMBUS.Blood Platelets: Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.Angioplasty, Balloon, Coronary: Dilation of an occluded coronary artery (or arteries) by means of a balloon catheter to restore myocardial blood supply.Receptors, Purinergic P2: A class of cell surface receptors for PURINES that prefer ATP or ADP over ADENOSINE. P2 purinergic receptors are widespread in the periphery and in the central and peripheral nervous system.Coronary Vessels: The veins and arteries of the HEART.Coronary Artery Disease: Pathological processes of CORONARY ARTERIES that may derive from a congenital abnormality, atherosclerotic, or non-atherosclerotic cause.Interferon-gamma: The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.Coronary Angiography: Radiography of the vascular system of the heart muscle after injection of a contrast medium.Th1 Cells: Subset of helper-inducer T-lymphocytes which synthesize and secrete interleukin-2, gamma-interferon, and interleukin-12. Due to their ability to kill antigen-presenting cells and their lymphokine-mediated effector activity, Th1 cells are associated with vigorous delayed-type hypersensitivity reactions.Th2 Cells: Subset of helper-inducer T-lymphocytes which synthesize and secrete the interleukins IL-4, IL-5, IL-6, and IL-10. These cytokines influence B-cell development and antibody production as well as augmenting humoral responses.Atherosclerosis: A thickening and loss of elasticity of the walls of ARTERIES that occurs with formation of ATHEROSCLEROTIC PLAQUES within the ARTERIAL INTIMA.

*Fluorescence in situ hybridization

Flow-FISH uses flow cytometry to perform FISH automatically using per-cell fluorescence measurements. Microfluidics-assisted ... or imaged simultaneously with a fluorescent antibody assay. The technology has potential applications in cancer diagnosis, ... Single-molecule RNA FISH assays can be performed in simplex or multiplex, and can be used as a follow-up experiment to ... Separate but compatible signal amplification systems enable the multiplex assay (up to two targets per assay). Signal ...

*High-content screening

Drug discovery High-throughput screening Drug discovery hit to lead Microscopy Flow cytometry Live single-cell imaging Haney SA ... some also have humidity control for longer term live cell imaging), a built in pipettor or injector for fast kinetic assays, ... This technology allows a (very) large number of experiments to be performed, allowing explorative screening. Cell-based systems ... Edwards BS, Oprea T, Prossnitz ER, Sklar LA (August 2004). "Flow cytometry for high-throughput, high-content screening". Curr ...

*Chemical biology

On the other hand, biological assays can be quite complicated to perform in vivo with high-throughput like efficiency and there ... or fluorescently conjugated antibodies and flow cytometry to provide a detailed, specific, quantitative results with excellent ... The optical probe is then incorporated into the system to image the fate of the altered biomolecules. Other methods of ... These assays include top agar overlay assays where antibiotics generate zones of growth inhibition against test microbes, and ...

*Miltenyi Biotec

... flow cytometry, cell culture, molecular analysis, clinical applications and small animal imaging. These technologies support a ... Since 1997, more than 35,000 clinical treatments on the basis of MACS Technology have been performed. CryoMACS Freezing Bags ... MACS Cytokines are designed for demanding applications such as cell culture, differentiation studies, and functional assays ... flow cytometry, molecular applications, cell culture up to GMP grade, preclinical imaging, clinical-grade cell preservation, ...

*Affimer

... immunohistochemistry and flow cytometry. Affimer reagents that inhibit protein-protein interactions can also be produced with ... The use of in vitro screening techniques allows affinity maturation to be performed to achieve greater binding affinities and ... "Sensitive Affimer and antibody based impedimetric label-free assays for C-reactive protein". Anal. Chem. 84 (15): 6553-60. doi: ... and click chemistry has been used to conjugate Affimer proteins to MRI contrast reagents for the purposes of targeted imaging. ...

*High throughput biology

Edwards BS, Oprea T, Prossnitz ER, Sklar LA (August 2004). "Flow cytometry for high-throughput, high-content screening". Curr ... The basic idea is to take methods normally performed on their own and do a very large number of them without impacting their ... High throughput cell biology has a definite focus on the cell, and methods accessing the cell such as imaging, gene expression ... Giuliano KA, Haskins JR, Taylor DL (August 2003). "Advances in high content screening for drug discovery". Assay Drug Dev ...

*Assay

... optic Flow cytometric cell or particle counters, or coulter/impedance principle based cell counters Imaging assays, that ... an assay may be: An end point assay, in which a single measurement is performed after a fixed incubation period; or A kinetic ... involve image analysis manually or by software: Cytometry: When the size statistics of cells is assessed by an image processor ... DNase footprinting assay Filter binding assay Gel shift assay Bicinchoninic acid assay (BCA assay) Bradford protein assay Lowry ...

*Cell counting

Flow cytometry is by far the most sophisticated and expensive method for cell counting. In a flow cytometer the cells flow in a ... Generally performs with a constant error rate as an off-line (batch) type process. A range of image classification techniques ... for instance the Luria-Delbrück experiment or the gentamicin protection assay). In addition, the enumeration of colonies on ... images over which a statistical classification algorithm is used to perform automated cell detection and counting as an image ...

*Q-FISH

Similar to Q-FISH, Flow-FISH is an adaptation of Q-FISH that combines the use of PNAs with flow cytometry. In this method, Flow ... The two images can then be superimposed to generate a combined image. This image can then be karyotyped or assigned ... PNA-FISH assays have been developed for identifying and diagnosing infectious diseases in a rapid manner within the clinic. ... Since the test can be performed relatively quickly, the test is being considered for use in hospitals where hospital-acquired ...

*Flow cytometry

A flow cytometer is similar to a microscope, except that, instead of producing an image of the cell, flow cytometry offers high ... Annexin A5 affinity assay, a test for cells undergoing apoptosis, often uses flow cytometry Cell cycle analysis Coulter counter ... Once the data is collected, there is no need to stay connected to the flow cytometer and analysis is most often performed on a ... Cytometry Dielectrophoresis Flow Cytometry Standard Mass cytometry Microfluorimetry Flow Cytometry in Microbiology" by David ...

*Glutathione

... as well as be applied in flow cytometry to perform bulk measurements in high throughput manner. Organelle-targeted RT probe has ... White CC, Viernes H, Krejsa CM, Botta D, Kavanagh TJ (July 2003). "Fluorescence-based microtiter plate assay for glutamate- ... "High-resolution imaging of redox signaling in live cells through an oxidation-sensitive yellow fluorescent protein". Science ... but also be applied in flow cytometry to perform bulk measurements. The RealThiol (RT) probe is the second-generation ...

*Microparticle

Particle image velocimetry - Solid microspheres used for flow visualization, typically with density matching that of the fluid ... microparticles carry useful information and can be detected and characterized by flow cytometry. Although the generally ... New applications for microspheres are discovered every day, below are just a few: Assay - Coated microspheres provide measuring ... Microspheres made from highly transparent glass can perform as very high quality optical microcavities or optical ...

*Flow cytometry bioinformatics

"Standardization of cytokine flow cytometry assays". BMC Immunology. 6: 13. doi:10.1186/1471-2172-6-13. PMC 1184077 . PMID ... are thus a critical step in many flow cytometry analyses. Single-marker normalization can be performed using landmark ... "Cytometry Standards Continuum" (PDF). SPIE Proceedings. Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues ... Flow Cytometry Standard (FCS) was developed in 1984 to allow recording and sharing of flow cytometry data. Since then, FCS ...

*Garvan Institute of Medical Research

Animal Imaging Animal Research Biodata Visualisation Bioinformatics Clinical Research Facility Flow Cytometry Functional ... The Institute performed well, with a success rate of 26% for project grants, well ahead of the national average of 16.9%. ... fostered by the presence of the State Reference Laboratory for endocrine assays. A hormone radioimmunoassay service at Garvan ...

*Antibody

Antibodies are used in flow cytometry to differentiate cell types by the proteins they express; different types of cell express ... This study can be performed in vitro (Reactivity of the antibody or immunoconjugate should be determined with a quick-frozen ... For example, in biochemical assays for disease diagnosis, a titer of antibodies directed against Epstein-Barr virus or Lyme ... imaging of cancer. Targeted monoclonal antibody therapy is employed to treat diseases such as rheumatoid arthritis, multiple ...

*Pathology

... flow cytometry, and molecular-pathologic analysis. One of the greatest challenges of dermatopathology is its scope. More than ... Examples of important subdivisions in medical imaging include radiology (which uses the imaging technologies of X-ray ... An autopsy is typically performed by a coroner or medical examiner, often during criminal investigations; in this role, ... antibody based immunofluorescence tissue assays, molecular profiling of pathogens, and analysis of bacterial genes for ...

*Arabidopsis thaliana

Using Flow Cytometry Show Genome Size in Arabidopsis to be 157 Mb and thus 25% Larger than the Arabidopsis Genome Initiative ... AK Yetisen; L Jiang; J R Cooper; Y Qin; R Palanivelu; Y Zohar (May 2011). "A microsystem-based assay for studying pollen tube ... By wet-mounting seedlings in water or in culture media, plants may be imaged uninvasively, obviating the need for fixation and ... Ongoing research on Arabidopsis thaliana is being performed on the International Space Station by the European Space Agency. ...

*Survivin

In vitro assays and other tests were also performed to validate the idea of the occurrence of an actual immune response to ... Analysis by flow cytometry revealed an accumulation of cells in S phase upon treatment with resveratrol. The cells were also ... as physiological conditions were used to obtain the images. Apoptosis, the process of programmed cell death, involves complex ... performed a DNA methylation screening and identified that DNA methylation of IRAK3 plays a key role in survivin up-regulation ...

*Apoptosis

... phosphatidylserine exposure versus cell permeability by flow cytometry), cellular markers such as DNA fragmentation (flow ... label-free live cell imaging and quantification". Phase Holographic Imaging AB. Green, Douglas (2011). Means to an End: ... This assay is used to disrupt gene function by creating an intragenic gene fusion. When an APAF-1 gene trap is introduced into ... In order to perform analysis of apoptotic versus necrotic (necroptotic) cells, one can do analysis of morphology by time-lapse ...

*Colony-forming unit

OpenCFU is written in C++ and uses OpenCV for image analysis. NICE is a program written in MATLAB providing an easy way to ... This often requires the user to change the code in order to achieve an efficient work-flow, but can prove useful and flexible. ... Counting colonies is traditionally performed manually using a pen and a click-counter. This is generally a straightforward task ... Cytometry Part A. 77 (8): 790-7. doi:10.1002/cyto.a.20864. PMC 2909336 . PMID 20140968. Cai Z, Chattopadhyay N, Liu WJ, Chan C ...

*Michigan Medicine

... the Flow Cytometry Core (flow cytometry), the Functional Genomic Core (functional genomics), the Metabolic Phenotyping Core, ... The facility also is home to: Sleep Research Center MRI Simulator Brain Imaging Center Stress and Neuroendocrine Program The ... 1968 U-M physicians perform the first heart transplant in Michigan at University Hospital. 1969 C.S. Mott Children's Hospital ... discovery and development of HbA1c as a key assay in diabetes Josiah K. Lilly Jr. - Chairman and President of Eli Lilly and ...

*Reference ranges for blood tests

Determination of monocyte count by hematological analyzers, manual method and flow cytometry in polish population Central ... Taken from the assay method giving the lowest and highest estimate, respectively, from Table 2 in: Beltran L, Fahie-Wilson MN, ... A separate printable image is available for mass and molarity Smaller, narrower boxes indicate a more tight homeostatic ... Blood test results should always be interpreted using the reference range provided by the laboratory that performed the test. A ...

*Circulating tumor cell

FISH assays has been performed on CTC to as well as determination of IGF-1R, Her2, Bcl-2, [ERG (gene),ERG], PTEN, AR status ... The imaging and analysis technology also allows for the coordinates of every cell on a slide to be known so that a single cell ... Cytometry Part A. 71A (3): 132-142. doi:10.1002/cyto.a.20369. F. A. W. Coumans; C. J. M. Doggen; G. Attard; et al. (2010). "All ... "Computational Analysis of Microfluidic Immunomagnetic Rare Cell Separation from a Particulate Blood Flow". Analytical Chemistry ...
p300 (EP300) and CBP (CREBBP) are transcriptional coactivators with histone acetyltransferase activity. Various beta cell transcription factors can recruit p300/CBP, and thus the coactivators could be important for beta cell function and health in vivo. We hypothesized that p300/CBP contribute to the development and proper function of pancreatic islets. To test this, we bred and studied mice lacking p300/CBP in their islets. Mice lacking either p300 or CBP in islets developed glucose intolerance attributable to impaired insulin secretion, together with reduced alpha and beta cell area and islet insulin content. These phenotypes were exacerbated in mice with only a single copy of p300 or CBP expressed in islets. Removing p300 in pancreatic endocrine progenitors impaired proliferation of neonatal alpha and beta cells. Mice lacking all four copies of p300/CBP in pancreatic endocrine progenitors failed to establish alpha and beta cell mass postnatally. Transcriptomic analyses revealed significant ...
Diabetes or diabetes mellitus which is the correct medical term is a medical condition were the affected person lack the ability to regulate his or her blood glucose levels. This inability is directly due to the fact that the insulin producing cells, residing in the pancreas, cant meet the bodys demand for insulin. It is estimated that close to 200 million people are suffering from diabetes today and this number is predicted to double within 20 years. Of the approximately 200 million people suffering from diabetes today approximately 20 million are in dependent on daily injections of insulin. Being dependent on exogenous insulin is not only an inconvenience it also increase the risk for several medical complications such as stroke, heart disorders, kidney failure, retinopathy, atherosclerosis and impaired wound healing. The major risk factor for all these complications is long periods of high blood sugar levels that is damaging to thin blood vessels and nerves. Even in the best of situations ...
TY - JOUR. T1 - Effect of cerulein hyperstimulation on the paracellular barrier of rat exocrine pancreas. AU - Fallon, Michael B.. AU - Gorelick, Fred S.. AU - Anderson, James M.. AU - Mennone, Albert. AU - Saluja, Ashok. AU - Steer, Michael L.. PY - 1995/6. Y1 - 1995/6. N2 - Background/Aims: Cerulein-induced pancreatitis causes a rapid increase in pancreatic enzyme levels in serum and decreases in pancreatic duct secretion and interstitial edema. One mechanism to explain these early events is disruption of the actin tight junction paracellular seal of acinar and intralobular pancreatic duct cells. Methods: To examine the paracellular barrier of the proximal exocrine pancreas, rats were hyperstimulated with 5.0 μg · kg-1 · h-1 of cerulein. Actin was visualized with rhodamine phalloidin and by electron microscopy and tight junctions were visualized with antibodies to the tight-junction protein ZO-1. Paracellular permeability was measured by movement of horseradish peroxidase from interstitium ...
pab pancreas deoxyribonuclease i rabbit pab against bovine pancreas deoxyribonuclease i | order pab pancreas deoxyribonuclease i rabbit pab against bovine pancreas
Hedgehog (Hh) signaling regulates cell proliferation and differentiation in various organs during embryogenesis. In the pancreas, the role of this pathway is complex. At early stages, Hh signaling is excluded from the pancreas and ectopic activation of the pathway impairs pancreas formation by disturbing mesenchymal-epithelial interactions. In contrast to this inhibitory role, our preliminary data suggest a positive, cell autonomous role for Hh signaling during endocrine cell formation and function. The exact nature of this novel, cell autonomous activity remains to be elucidated. Little information is also available about the upstream mechanisms that regulate Hh signaling in pancreatic epithelium. Recent results have revealed that primary cilia, cellular appendages found on many cell types, control the level of Hh signaling activity. Primary cilia are present on adult duct and endocrine cells within the pancreas, the same cell types that are marked by expression of Ptc, a transcriptional target ...
Introduction: Minimally invasive approches in treatment of acute necrotizing pancreatitis and/or infected pancreatic necrosis gain a notable advantage compared with open surgery.. Aim: We present our experience in treatment of acute necrotizing pancreatitis by an original minimally invasive retroperitoneal necrosectomy technique, evaluate feasibility and safety of this method, compare our results to other studies.. Patients and methods: A retrospective analysis of 22 patients with acute necrotizing pancreatitis and large fluid collections in retroperitoneal space was performed. All patients underwent retroperitoneal necrosectomy as an initial interventional procedure in treatment of infected pancreatic necrosis.. Results: Sixteen males and six females aged between 24 and 60 with an average age of 42.59 ±7.3 years were included. Alcohol abuse was an etiologic factor of acute necrotizing pancreatitis for 18 patients (81.8%). Average time between diagnosis and performance of ...
Although the structure of Human Pancreatic Lipase has been documented through the X-ray crystallography, the knowledge about the molecular rearrangement and dynamic equilibrium in the structure (particularly in the catalytic triad and lid domains) is very scanty. The structural fluctuations and conformational changes undergo by Human Pancreatic Lipase (HPL) with and without colipase were computationally investigated through molecular dynamics simulation technique using GROMACS 2018.4, MOE 2016.0801 and VMD softwares in order to gain insight into the complex transitions at different domains. The structural stability was revealed vis-a-vis Root Mean Square Deviation (RMSD) and Root Mean Square Fluctuations (RMSF) plots. The levels of compactness/folding and conformational changes of the protein were determined using Radius of gyration and secondary analysis respectively. Salt bridge analysis gives more ionic pairs interactions than experimentally determined results. Results show that though both proteins
Pancreatic Stem Cells Found in Adult Mice Thursday, 24 January 2008 Just as many scientists had given up the search, researchers have discovered that the pancreas does indeed harbour stem cells with the capacity to generate new insulin-producing beta cells. If the finding made in adult mice holds for humans, the newfound progenitor cells will represent "an obvious target for therapeutic regeneration of beta cells in diabetes," the researchers report in the Jan. 25 issue of Cell, a publication of Cell Press. "One of the most interesting characteristics of these [adult] progenitor cells is that they are almost indistinguishable from embryonic progenitors," said Harry Heimberg of the JDRF Center at Vrije Universiteit Brussel in Belgium and the JDRF Center for Beta Cell Therapy in Diabetes. "In terms of their structure and gene expression, there are no major differences. They look and behave just like embryonic beta cell progenitors." Insulin is required for cells to take up blood sugar, the bodys ...
Pancreas Cancer Treatment of Alternative Pancreas Cancer Alternative Neuro Acupuncture Treatment and Pancreas Cancer Herbal Herbs Alternative Medicine Treatment on Pancreas Cancer Treatment Medical Center Pancreas Cancer Remedies
Bile salt-dependent lipase (or BSDL), also known as carboxyl ester lipase (or CEL) is an enzyme produced by the adult pancreas and aids in the digestion of fats. Bile salt-stimulated lipase (or BSSL) is an equivalent enzyme found within breast milk. BSDL has been found in the pancreatic secretions of all species in which it has been looked for. BSSL, originally discovered in the milk of humans and various other primates, has since been found in the milk of many animals including dogs, cats, rats, and rabbits. More than 95% of the fat present in human milk and in infant formulas is in the form of triacylglycerols (TG). In adults, TGs are thought to be broken down or hydrolyzed mainly by the colipase-dependent lipase enzyme. In the newborn, CDL activity in the duodenum is lower than in adults. Both BSDL and BSSL have a broad substrate specificity and, like CDL, are capable of hydrolyzing triacylglycerides (in addition to phospholipids, esters of cholesterol, and lipid-soluble vitamins). In ...
Accessory pancreas is a rare condition in which small groups of pancreatic cells are separate from the pancreas. They may occur in the mesentery of the small intestine, the wall of the duodenum, the upper part of the jejunum, or more rarely, in the wall of the stomach, ileum, gallbladder or spleen. The condition was first described by Klob in 1859. Accessory pancreas is a small cluster of pancreas cells detached from the pancreas and sometimes found in the wall of the stomach or intestines. After researching accessory pancreas at the University of Louisville medical library, I found a list of medical cases and the doctors that operated on the patients with this condition. In 1904, Dr. A. S. Warthin found 47 cases in the literature and added 2. Up to 1921, 31 cases were added. Twelve cases were found at operation. A number of patients have been operated upon for this trouble since then. E. J. Horgan found 2 cases in 321 consecutive autopsies. Locality of Accessory Pancreas, A. S. Warthin, 1904: ...
Description of disease Pancreas divisum. Treatment Pancreas divisum. Symptoms and causes Pancreas divisum Prophylaxis Pancreas divisum
Chronic pancreatitis is an inflammatory disease of the exocrine pancreas characterised by the replacement of pancreatic parenchyma by fibrosis.1 2 Apart from fibrosis, the pancreatic parenchyma may also exhibit enlarged ducts, infiltration by inflammatory cells, the formation of duct-like tubular complexes, regions of acinar cell degeneration, and duct cell proliferation.3 In human acute and chronic pancreatitis, pancreatic cancer, and animal models of acute pancreatitis, duct-like structures, referred to as tubular complexes, have been described.4 8 Tubular complexes evolve as the acinar cells decrease in height and lose acinar cell specific antigens, paralleled by increased expression of duct cell markers.9 39 The molecular alterations underlying the fibrotic changes and the formation of these duct-like complexes are largely unknown. Recently, tyrosine kinase receptors, comprising the large family of growth factor receptors, have been shown to be overexpressed in the pancreas of patients with ...
... provided by the Genemart Bioscience Inc.,The Product Intro:DNase I, Bovine Pancreas, >2000U/MG
article{6854376, abstract = {Expansion of pancreatic beta cells in vivo or ex vivo, or generation of beta cells by differentiation from an embryonic or adult stem cell, can provide new expandable sources of beta cells to alleviate the donor scarcity in human islet transplantation as therapy for diabetes. Although recent advances have been made towards this aim, mechanisms that regulate beta cell expansion and differentiation from a stem/progenitor cell remain to be characterized. Here, we describe a protocol for an injury model in the adult mouse pancreas that can function as a tool to study mechanisms of tissue remodeling and beta cell proliferation and differentiation. Partial duct ligation (PDL) is an experimentally induced injury of the rodent pancreas involving surgical ligation of the main pancreatic duct resulting in an obstruction of drainage of exocrine products out of the tail region of the pancreas. The inflicted damage induces acinar atrophy, immune cell infiltration and severe ...
Transplants of insulin-secreting pancreas cells is a dream of medical science and medicos. A recent study shows that these grafts can actually allay some of the misery of patients with extreme diabetes.. Find the best Cyber Monday Deals 2017. They could actually prove to be a life-saving device. These transplants may get approval soon in the United States. The transplants are found in many other countries, yet they are only found in research studies in the United States. Starting from yesterday, the medical authorities have approved of the limited use of these transplants for diabetics who are in danger of seizures and death.. The sinking blood sugar levels are responsible for such a state of affairs. Most of these sufferers have Type 1 diabetes. The treatment of diabetes at the cellular level is an actuality and allays many of the nasty symptoms faced by patients.. In Type 1 diabetes, the immune system attacks the insulin-producing cells of the pancreas. Insulin is responsible for converting ...
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The human pancreas has vital roles in the regulation of glucose homeostasis and digestion and its dysfunction leads to pancreatic disease as diabetes and pancreatitis. Recent researches have highlighted the novel roles of a local renin-angiotensin system (RAS) in the pancreas and its clinical relevances; its inapprporiate activation leads to pancreatic endocrine and exocrine disease, notably type 2 diabetes. As such, manipulation of the overactive RAS may improve pancreatic islet cell function, cell mass and insulin sensitivity, as well as enhancing the growth and differentiation of pancreatic stem cells. Blockade of the RAS may be protective against pancreatitis and pancreatic cancer. The research outcome of current progress on the RAS in the pancreas should provide an alternative approach to preventing and treating, as well as curing pancreatic disease. This book discusses the progress of research on the renin-angiotensin system in the pancreas from the science to the bedside, providing a ...
Hamster Pancreatic Epithelial Cells from Creative Bioarray are isolated from pancreatic tissue of pathogen-free laboratory mice. Hamster Pancreatic Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarrays Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended ...
Cynomolgus Monkey Pancreatic Epithelial Cells from Creative Bioarray are isolated from normal Cynomolgus Monkey pancreatic tissue. Cynomolgus Monkey Pancreatic Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarrays Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended ...
Pancreas divisum is the most common birth defect of the pancreas. In many cases, this defect goes undetected and causes no problems. The cause of the defect is unknown.. As a baby develops in the womb, 2 separate pieces of tissue join together to form the pancreas. Each part has a tube, called a duct. When the parts join together, a final duct, called the pancreatic duct, is formed. Fluid and digestive juices (enzymes) produced by the pancreas normally flow through this duct.. If the ducts do not join while the baby is developing in the womb, pancreas divisum results. Fluid from the 2 parts of the pancreas drains into separate areas of the upper portion of the small intestine (duodenum). This occurs in 5% to 15% of people.. If a pancreatic duct becomes blocked, swelling and tissue damage (pancreatitis) may develop.. ...
Pluripotent stem cells have the ability to differentiate into all cell and tissue types of the body, most notably shown in mice by the generation of chimeric animals. Directed differentiation of human pluripotent stem cells into various cell types in vitro has only recently become possible by applying developmental signals based on lessons learned from developmental biology in animals. Initial attempts to generate beta cells from human embryonic stem cells relied on spontaneous differentiation. By culturing embryonic bodies in stem cell media, Assady and colleagues reported the upregulation of insulin transcripts and occasional insulin‐positive cells, but most cells were not of pancreatic origin (Assady et al, 2001). To improve differentiation efficiency into insulin‐positive cells, developmental steps were sequentially targeted, progressing from definitive endoderm to primitive gut tube, through posterior foregut, pancreatic endoderm, pancreatic endocrine progenitors and, finally, to beta ...
Pancreatic exocrine insufficiency is an often-underestimated complication following pancreatic surgery. After recent advances in managing acute postoperative complications the focus of current research is now shifting onto the long-term complications following pancreatectomy. Weight loss and steatorrhea as typical symptoms have high influence on the quality of life in the postoperative period. Malnutrition-related symptoms occur late and are often misinterpreted. Enzyme replacement therapy is more or less the only possible treatment option, even though not many controlled trials have been performed in this field. In this review we summarized the pathophysiology, diagnosis, risk factors and treatment options of exocrine insufficiency and focus mainly on patients with pancreaticoduodenectomy (classical Whipple), pylorus-preserving pancreaticoduodenectomy (ppWhipple) or distal pancreatectomy. Incidence of pancreatic exocrine insufficiency after surgery depends mainly on the initial diagnosis, ...
TY - JOUR. T1 - Is pancreatitis a complication of endoscopic ultrasound guided fine needle aspiration of the pancreas?. AU - Gress, F.. AU - Ikenberry, S.. AU - Ciaccia, D.. AU - Kiel, J.. AU - Lehman, Glen. PY - 1997. Y1 - 1997. N2 - Introduction: Endoscopic ultrasound (EUS) guided fine needle aspiration (FNA) of the pancreas is increasingly being utilized to diagnose pancreatic masses. We have previously reported 2 cases of clinical pancreatitis following EUS guided FNA of the pancreas. Aim of Study: To determine the frequency of chemical and clinical pancreatitis following EUS guided FNA of the pancreas. Methods: All patients having EUS guided FNA biopsy of the pancreas were eligible. Exclusion criteria included: All patients with acute pancreatitis or having ERCP within 48 hours of the EUS FNA. Serum amylase and lipase levels were measured immediately prior to and 2 hours post EUS FNA. Patients were called 24-72 hours after FNA to follow up for increased pain, nausea, or vomiting suggesting ...
Abstract: Melatonin, an antioxidant, protects the pancreas against acute inflammation but, although this indole is released mainly at night, no study has been undertaken to determine circadian changes of plasma melatonin levels and the severity of acute pancreatitis. The aims of this study were: (a) to compare the severity of caerulein-induced pancreatitis (CIP) produced in the rat during the day and at the night, and (b) to assess the changes of plasma melatonin level and the activity of an antioxidative enzyme; superoxide dismutase (SOD), in the pancreas subjected to CIP during the day time and at night without or with administration of exogenous melatonin or its precursor; l-tryptophan. Rats were kept in 12 hr light/dark cycle. CIP was induced by subcutaneous infusion of caerulein (5 μg/kg/hr for 5 hr). Melatonin (5 or 25 mg/kg) or l-tryptophan (50 or 250 mg/kg) was given intraperitoneally 30 min prior to the start of CIP. CIP induced during the day time was confirmed by histological ...
TY - JOUR. T1 - Endoscopic retrograde pancreatography criteria to diagnose autoimmune pancreatitis. T2 - An international multicentre study. AU - Sugumar, Aravind. AU - Levy, Michael J.. AU - Kamisawa, Terumi. AU - Webster, George J M. AU - Kim, Myung Hwan. AU - Enders, Felicity T. AU - Amin, Zahir. AU - Baron, Todd H.. AU - Chapman, Mike H.. AU - Church, Nicholas I.. AU - Clain, Jonathan E.. AU - Egawa, Naoto. AU - Johnson, Gavin J.. AU - Okazaki, Kazuichi. AU - Pearson, Randall K.. AU - Pereira, Stephen P.. AU - Petersen, Bret Thomas. AU - Read, Samantha. AU - Sah, Raghuwansh P.. AU - Sandanayake, Neomal S.. AU - Takahashi, Naoki. AU - Topazian, Mark. AU - Uchida, Kazushige. AU - Vege, Santhi Swaroop. AU - Chari, Suresh T. PY - 2011/5. Y1 - 2011/5. N2 - Background: Characteristic pancreatic duct changes on endoscopic retrograde pancreatography (ERP) have been described in autoimmune pancreatitis (AIP). The performance characteristics of ERP to diagnose AIP were determined. Methods: The ...
Patients could develop endocrine and exocrine pancreatic insufficiency after acute pancreatitis (AP), but the morbidity, risk factors and outcome remain unclear. The aim of the present study was to evaluate the incidence of endocrine and exocrine pancreatic insufficiency after AP and the risk factors of endocrine pancreatic insufficiency through a long-term follow-up investigation. Follow-up assessment of the endocrine and exocrine function was conducted for the discharged patients with AP episodes. Oral Glucose Tolerance Test (OGTT) and faecal elastase-1(FE-1) test were used as primary parameters. Fasting blood-glucose (FBG), fasting insulin (FINS), glycosylated hemoglobin HBA1c, 2-h postprandial blood glucose (2hPG), Homa beta cell function index (HOMA-β), homeostasis model assessment of insulin resistance (HOMA-IR) and FE-1 were collected. Abdominal contrast-enhanced computed tomography (CECT) was performed to investigate the pancreatic morphology and the other related data during ...
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Since acinar cell carcinoma(ACC)of pancreas is rare, we sometimes meet a case hard to make diagnosis before surgery. We here reported a case of ACC of pancreas with extensive intraductal growth to the main pancreatic duct and the branch of the pancreatic duct. A 43-year-old man visit a clinic with a concern of uncomfortable feeling on left side abdomen. CT/MRI examination showed a ischemic tumor, 38×25 mm, in the body-tail of the pancreas, and the tumor infiltrated to left renal capsule. ERCP showed the interruption and stenosis of the pancreatic duct at the point adjacent to tumor. Cytological diagnosis of the pancreatic juice was performed, but malignant cells were not detected(Class III ). The tumor abnormally accumulated FDGin PET-CT examination(SUVmax 3.3). We diagnosed the tumor PDAC with infiltrating to the left renal capsule, and the distal pancreatectomy was performed. The pathological examination of the resected specimen showed that the tumor progressed into main ...
TY - JOUR. T1 - Effect of germinated brown rice extracts on pancreatic lipase, adipogenesis and lipolysis in 3T3-L1 adipocytes. AU - See Meng, Lim. AU - Goh, Yong Meng. AU - Kuan, Wen Bin. AU - Loh, Su Peng. PY - 2014/11/3. Y1 - 2014/11/3. N2 - Background: This study investigated anti-obesity effects of seven different solvent (n-hexane, toluene, dicholoromethane, ethyl acetate, absolute methanol, 80% methanol and deionized water) extracts of germinated brown rice (GBR) on pancreatic lipase activity, adipogenesis and lipolysis in 3T3-L1 adipocytes. Methods: GBR were extracted separately by employing different solvents with ultrasound-assisted. Pancreatic lipase activity was determined spectrophotometrically by measuring the hydrolysis of p-nitrophenyl butyrate (p-NPB) to p-nitrophenol at 405 nm. Adipogenesis and lipolysis were assayed in fully differentiated 3T3-L1 adipocytes by using Oil Red O staining and glycerol release measurement. Results: GBR extract using hexane showed the highest ...
Pancreas Cancer Surgery resources with links to Pancreas Cancer Surgery specialist for diagnosis of Pancreas Cancer Surgery, Pancreas Cancer Surgery symptoms and Pancreas Cancer Surgery treatment. We help answer all your qestions about Pancreas Cancer Surgery.
The source of new beta-cells in adult human pancreas remains incompletely elucidated with recent studies in rodents providing evidence for neogenesis from progenitor cells in addition to self-replication. The aim of this study was to investigate expression of pluripotency-associated stem cell markers in proliferative cultures derived from adult human pancreas.Human pancreatic tissue was obtained from deceased donors following ethical approval and relative consent. Islet-enriched fraction was separated from the retrieved organ by digestion and density gradient centrifugation. Dissociated cells were seeded in adherent culture forming proliferative islet survivor cells(ISCs). These were characterized at fifth passage by RT-PCR, immunofluorescence staining, FACS, Western blot and transfection studies with an OCT4 promoter-driven reporter.Nuclear expression of the pluripotency-associated stem cell marker complex OCT4 / SOX2 / NANOG was confirmed in ISCs. The phenotype constituted approximately 8% ...
Pancreatic organogenesis proceeds through several crucial regulatory stages (Cleaver and MacDonald, 2010; Rieck et al., 2012): (1) specification and emergence of the pancreatic epithelial buds from the distal foregut endoderm; (2) expansion of an early pancreatic progenitor cell population and developmental commitment; (3) formation of a more restricted multipotent progenitor cell (MPC) population for endocrine, ductal and acinar tissues; (4) separation of committed pre-acinar cells at the tips of epithelial branches and a bipotent cell-population for islets and ducts in more central trunk domains; (5) separation of the endocrine and ductal programs, morphogenetic resolution of a precursor ductal plexus and completion of cell type-specific differentiation. This process creates three interrelated tissues: the islets of Langerhans (endocrine cells that produce polypeptide hormones, including insulin and glucagon), acini (exocrine cells that produce and secrete hydrolytic digestive enzymes) and ...
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The pancreas is a vertebrate-specific endodermal organ executing major functions in food digestion and glucose homeostasis. The mature organ is composed of an exocrine compartment with acinar and duct cells that produce and transport digestive enzymes into the gut, and an endocrine compartment from which metabolism-regulating peptide hormones including insulin are secreted into the blood stream. Studies on pancreas regeneration have focused mainly on the endocrine compartment, with the aim of gaining knowledge on β-cell regeneration. More recently, exocrine pancreas regeneration has been receiving more attention in the context of cancer and diabetes research because experimental models revealed a high capacity for cell fate plasticity (Murtaugh and Keefe, 2015).. Regeneration of exocrine tissue has been mainly studied in mouse models, where the loss of cells can be induced by caerulein treatment or partial duct ligation (Aghdassi et al., 2011; Lerch and Gorelick, 2013). Using these procedures, ...
Adipose Tissue Development Endocrine Development can be very useful guide, and Adipose Tissue Development Endocrine Development play an important role in your products. The problem is that once you have gotten your nifty new product, the Adipose Tissue Development Endocrine Development gets a brief glance, maybe a once over, but it often tends to get discarded or lost with the original packaging. ...
TY - JOUR. T1 - Matrix metalloproteinase-7 is expressed by pancreatic cancer precursors and regulates acinar-to-ductal metaplasia in exocrine pancreas. AU - Crawford, Howard C.. AU - Scoggins, Charles R.. AU - Kay Washington, M.. AU - Matrisian, Lynn M.. AU - Leach, Steven D.. PY - 2002. Y1 - 2002. N2 - In gastrointestinal epithelium, metaplastic conversion between predominant cell types is associated with an increased risk of neoplasia. However, the mechanisms regulating metaplastic transitions in adult epithelia are largely undefined. Here we show that matrix metalloproteinase-7 (MMP-7) is expressed not only in the majority of human pancreatic ductal adenocarcinoma specimens, but also in human pancreatic intraepithelial neoplasia and metaplastic duct lesions in human and mouse. In a mouse model of pancreatic acinar-to-ductal metaplasia, MMP-7 progressively accumulates during the metaplastic transition, resulting in a concomitant increase in solubilization of Fas ligand (FasL). Under identical ...
TY - JOUR. T1 - Rejection with duodenal rupture after solitary pancreas transplantation. T2 - An unusual cause of severe hematuria. AU - Esterl, R. M.. AU - Stratta, R. J.. AU - Taylor, R. J.. AU - Radio, Stanley J. PY - 1995/1/1. Y1 - 1995/1/1. N2 - A common urologic complication after pancreas transplantation with bladder drainage is hematuria. However, hematuria is usually mild and self-limiting and rarely requires open surgical intervention. We report an unusual case of refractory hematuria that began 17 days after solitary pancreas transplantation due to severe rejection. The patient developed severe duodenal segment swelling with mucosal rupture that eventually required operative therapy. This case is illustrative of the diagnosis and management of gross hematuria after pancreas transplantation and demonstrates the limitations of cystoscopic techniques. This unusual complication must be considered in the differential diagnosis of refractory hematuria with pancreas allograft dysfunction and ...
Review question How should people with necrotising pancreatitis be treated?. Background The pancreas is an organ in the abdomen (tummy) that secretes several digestive enzymes (substances that enable and speed up chemical reactions in the body) into the pancreatic ductal system, which empties into the small bowel. It also contains the Islets of Langerhans, which secrete several hormones including insulin (helps regulate blood sugar). Acute pancreatitis is sudden inflammation of the pancreas and can lead to destruction of the pancreas (pancreatic necrosis). Pancreatic necrosis can be infected or non-infected (sterile). Pancreatic necrosis can lead to failure of other organs, such as the lungs and kidneys, and is a life-threatening illness. The main treatments for pancreatic necrosis include removal of the dead tissue (debridement or necrosectomy), peritoneal lavage (washing dead tissue out of the abdomen, drainage (inserting a tube or drain to drain out the fluid collection around the ...
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CONTEXT: Acute pancreatitis in ectopic pancreatic tissue is an uncommon cause of acute abdominal pain and can be difficult to diagnose on imaging. Our aim is to raise awareness and aid in the diagnosis of this entity by highlighting helpful dynamic contrast-enhanced MRI imaging findings. CASE REPORT: We report a 51-year-old man with acute onset epigastric pain presented to ER. With the presence of elevated serum lipase, the clinical diagnosis of acute pancreatitis was made. Contrast enhanced CT demonstrated normal pancreas and a focal mass at the duodenojejunal flexure, mimicked a neoplasm. Subsequent dynamic contrast enhanced MR images demonstrated enhancement pattern of the lesion similar to the native pancreatic tissue enhancement, a finding raised the possibility of acute pancreatitis in ectopic pancreatic tissue, but tumor was not excluded. Finally, patient undergone surgical bowel resection including the suspected mass that was proved as an ectopic pancreatic tissue on microscopic ...
Maintaining islet viability after pancreas procurement is critical for optimal graft function and survival in islet transplantation. It is recently reported that islet yield and function were significantly higher for pancreas preserved in the modified solution added with trypsin inhibitor (ulinastatin). In this study, the FDA-cleared LifePort ® platform was applied for porcine pancreas preservation, which provided a pulsatile circulation of a perfusate with KPS1 solution added with ulinastatin (100×103 U/L) during preservation. In the control group, pancreas was preserved by traditional cold storage. After 24 hours, the pancreas was distended by intraductal collagenase NB injection and normothermically digested. The isolated islets were purified by a continuous density-gradient centrifugation. The islet yield, islet viability, stimulation index and postpreservation edema were compared between the ulinastatin group (n=7) and control group (n=8). There was difference of islet yield (IEQ/g of ...
The etiology and pathogenesis of pancreatitis remains unclear. In the presence of pancreatic inflammation, metabolite abnormalities appear before transformation of tissue structure and changes in functions occur. Detection of abnormalities in metabolite levels facilitates a greater understanding of the pathophysiological events and aids in the early diagnosis of the disease. In this study, metabolic profiles from the pancreas of Wistar rats were examined using high-resolution proton magic angle spinning nuclear magnetic resonance (MAS NMR) spectroscopy to investigate the metabolite indicator(s) of acute necrotizing pancreatitis (ANP) and chronic pancreatitis (CP). The animals were divided into three groups: those with histologically confirmed ANP (n=7), those with CP (n=6) and a control group (n=9). The processed NMR spectra were analyzed using principal component analysis (PCA) to extract characteristic metabolites of ANP and CP. Levels of leucine, isoleucine and valine were increased in the ...
Solid pseudopapillary tumors (SPTs) are unusual neoplasms that mostly occur in the pancreas, and predominantly affect young women. As a low-grade malignant neoplasm of the exocrine pancreas, they occasionally metastasize, usually to the liver or peritoneum. It has been reported that ,1% of SPTs are primary extrapancreatic SPTs. In the present study, we present two rare, but conspicuous extrapancreatic SPTs. Both occurred in young women, and showed good prognoses following surgery. One was a recurrent SPT of the pancreas that metastasized to the ovary, and the other was a distinct primary neoplasm that arose in the retroperitoneal area. The pathological features of the two tumors, including solid and pseudopapillary growth patterns with pale or eosinophilic cytoplasm, were characteristic of SPTs of the pancreas. However, in the case of the metastatic ovarian tumor, focal necrosis and an increased nuclear-to-cytoplasmic ratio were observed. The presence of positive nuclear-cytoplasmic β-catenin, ...
Developmentally, the pancreas and liver are closely related and pathological conditions − including elevated glucocorticoid levels − result in the appearance of hepatocytes in the pancreas. The role of the WNT signalling pathway in this process has been examined in the model transdifferentiating pancreatic acinar AR42J-B-13 (B-13) cell. Glucocorticoid treatment resulted in a transient loss of constitutive WNT3a expression, phosphorylation and depletion of β-catenin, loss of β-catenin nuclear localisation, and significant reductions in T-cell factor/lymphoid enhancer factor (Tcf/Lef) transcriptional activity before overt changes in phenotype into hepatocyte-like (B-13/H) cells. A return to higher Tcf/Lef transcriptional activity correlated with the re-expression of WNT3a in B-13/H cells. β-catenin knock down alone substituted for and enhanced glucocorticoid-dependent transdifferentiation. Overexpression of a mutant β-catenin (pt-Xβ-cat) protein that blocked glucocorticoid-dependent ...
RESULTS: The acinar cell apoptotic index (AI) and proliferation index were higher in chronic pancreatitis than controls. T lymphocytes diffusely infiltrated fibrous bands and acini but rarely islets. Acinar Bcl-2 expression exceeded islet expression in chronic pancreatitis and controls while Bax was strongly expressed by a subset of islet cells and weakly by centroacinar cells. Islet Fas and Fas ligand expression exceeded acinar expression in chronic pancreatitis and controls. Acinar Rb expression was higher in chronic pancreatitis than in controls. Islets in chronic pancreatitis and controls showed intense TIMP-1 and TIMP-2 expression ...
TY - JOUR. T1 - Pathophysiology of hyperinsulinemia following pancreas transplantation. T2 - Altered pulsatile versus basal insulin secretion and the role of specific transplant anatomy in dogs. AU - Earnhardt, Richard C.. AU - Veldhuis, Johannes D. AU - Cornett, Greg. AU - Hanks, John B.. AU - Andersen, Dana K.. AU - Brunicardi, F. Charles. AU - Thomas, Francis T.. AU - Najarian, John S.. PY - 2002/10. Y1 - 2002/10. N2 - Objective: To evaluate the effect of the anatomical alterations of the pancreas required for transplantation on pulsatile insulin secretion. Summary Background Data: Pancreas transplantation involves anatomical changes that have unknown consequences on glucose homeostasis. Pancreas transplant patients are free of exogenous insulin requirements, yet appear to have endogenous hyperinsulinemia. The effect of surgical alterations on posttransplant insulin release is not completely known, specifically with regards to possible alterations in patterns of pulsatile release. Methods: ...
Preparation of the Pancreatic Lobules. After an overnight fast with free access to water, the animals were killed by cervical dislocation and the pancreatic lobules were obtained. The pancreatic lobules were prepared according to a method previously described [17]. In brief, the pancreas was quickly removed and immersed in modified calcium-free HEPES (25 mmol/L)-buffered Ringer solution with the following composition (Sigma-Aldrich, St. Louis, MO, USA): NaCl 113 mmol/L, HEPES 25 mmol/L, KCl 4.7 mmol/L, MgCl2 1.2 mmol/L, D-glucose 5.6 mmol/L, sodium pyruvate 5 mmol/L, sodium fumarate 5 mmol/L, sodium glutamate 5 mmol/L, and L-glutamine 2 mmol/L. The medium also contained 2% (v/v) essential amino acids, 1% (w/v) BSA, and 0.1% (w/v) soybean trypsin inhibitor. The solution was maintained at pH 7.4, while being continuously gassed with 100% O2 at 37 C. Whole pancreatic lobules were spread out by injecting calcium-free HEPES-Ringer solution gassed with 100% O2 into the loose connective tissue. The ...
View Notes - pancreas from BIO 2400 at Texas State. Pancreas: Gross Anatomy The pancreas and liver are the two large, extramural glands of the digestive system. The pancreas is a elongated organ,
Injury to pancreatic acinar cells is thought to be the initiating point for the progression of acute pancreatitis, and, therefore, they represent an ideal experimental system to study the mechanisms of experimental acute pancreatitis (Bhatia et al., 2005). In experimental acute pancreatitis models, SP has been shown to be up-regulated and plays a proinflammatory role (Lau and Bhatia, 2006). The use of PPT-A gene knockout models and pharmacological antagonism of NK1R reduced the severity of AP and its associated lung injury, showing the importance of SP-NK1R interaction in AP (Bhatia et al., 1998; Lau et al., 2005). SP and NK1R are expressed in the pancreatic acinar cells, and this expression could contribute to the overall outcome of caerulein-induced AP.. Our results showed that treatment of pancreatic acinar cells with caerulein up-regulated gene expression of PPT-A and NK1R and also peptide levels of SP. At a physiological concentration of caerulein (10−10 M), the expression of PPT-A, NK1R, ...
https://doi.org/10.18632/oncotarget.23619 Dominique Lombardo, Françoise Silvy, Isabelle Crenon, Emmanuelle Martinez, Aurélie Collignon, Evelyne Beraud, Eric Mas
Cytosolic free calcium concentrations ([Ca2+]i) and amylase secretion were measured in isolated rat pancreatic acini loaded with the intracellularly trapped fluorescent indicator quin2. Both caerulein and carbamoylcholine caused a rapid increase in [Ca2+]i, with a maximal 3-fold increase at 10(-9) M-caerulein and 10(-4) M-carbamoylcholine. However, caerulein (10(-12) M and 10(-11) M) as well as carbamoylcholine (10(-7) M) caused a significant stimulation of amylase release, while not inducing any detectable rise in [Ca2+]i. Changes in [Ca2+]i after addition of either secretagogue were transient and did not last more than 2-3 min. By contrast, when amylase secretion was monitored as a function of time, two distinct secretory phases could be observed upon addition of either carbamoylcholine (10(-5) M) or caerulein (10(-10) M). An initial, rapid phase (0-5 min) which caused a 6-7-fold increase above basal, followed by a sustained (5-30 min), but less marked, secretory rate (2-3-fold above basal). ...
Human Anatomy Pancreas - See more about Human Anatomy Pancreas, human anatomy pancreas, human anatomy pancreas function, human anatomy pancreas position, human anatomy pancreas symptoms
More than a century ago, a German pathologist noted at autopsies of patients who had succumbed to acute pancreatitis that their intrapancreatic digestive enzymes had been activated.1 His fateful observation engendered the surprisingly long-lived belief that pancreatitis is an autodigestive phenomenon resulting from the inappropriate activation of digestive enzymes within the pancreas itself. The past three decades have seen the development of several animal models of pancreatitis and remarkably, early intracellular trypsinogen activation has been observed consistently during the course of pancreatitis in all of them.2-5 Subsequently, pancreatitis research began to focus on the mechanisms of premature intracellular trypsinogen activation. We and others have shown that premature trypsinogen activation takes place in membrane-bound compartments resembling autophagic vesicles within which zymogen and lysosomal contents are colocalised.6-9 In these colocalisation vacuoles, the lysosomal protease ...
September 1, 2015 - The Pancreas Center at NewYork-Presbyterian/Columbia has been designated a Center for the Care and Treatment of Pancreatitis by the National Pancreas Foundation. Thirty centers across the nation have earned this designation; The Pancreas Center is the only NPF center in New York. In evaluating center for accreditation, the National Pancreas Foundation performs extensive audits of centers that treat pancreatitis and identifies those centers that provide the best possible outcomes. Centers must meet strict criteria including having an extensive team of expert specialists such as gastroenterologists, pancreas surgeons, interventional radiologists, and pathologists with expertise in gastrointestinal disease. Centers must offer patient-focused programs such as a pain management service, psychosocial support, diabetes/endocrinology programs, and other services. Centers must also be engaged in clinical trials for pancreatitis, have an auto-islet transplantation program, and ...
The pancreas is a soft oblong organ located in the upper central region of the abdominal cavity, just behind the lower surface of the stomach. It has three portions: an expanded medial portion called the head, a central portion called the body, and a tapering lateral portion called the tail. The head is partially encircled by the C-shaped duodenum, the first portion of the small intestine. The pancreas is both an exocrine gland and an endocrine gland. The exocrine portion of the pancreas consists of acinar cells (which account for about 99 percent of all secretory cells in the pancreas) that are organized into numerous small clusters called acini. The acinar cells secrete a clear fluid called pancreatic juice, which plays a critically important role in the digestion of food within the small intestine. The pancreatic juice is usually delivered to the duodenum by way of two ducts, the main pancreatic duct and the accessory pancreatic duct. (In some people, the accessory duct disappears during ...
Title:The Role of MicroRNAs in the Pancreatic Differentiation of Pluripotent Stem Cells. VOLUME: 3 ISSUE: 1. Author(s):Natalie Francis, Melanie Moore, Guy A. Rutter and Chris Burns. Affiliation:Endocrinology Section, Biotherapeutics Department, National Institute of Biological Standards and Control, Blanche Lane, South Mimms, Hertfordshire, EN6 3QG, UK.. Keywords:Differentiation, embryonic stem cells, endoderm, induced pluripotent stem cells, insulin, microRNA, pancreas, type 1 diabetes, type 2 diabetes.. Abstract:The generation of β-cells in vitro is an attractive option for cell therapy treatments for type 1 diabetes and also for the development of more accurate disease models. A number of studies have demonstrated that insulin-expressing cells can be generated by the in vitro differentiation of human pluripotent stem cells. However, to date, these differentiation protocols are often inefficient, time-consuming and highly variable. In many cases, this is a result of an incomplete ...
Alcoholic pancreatitis continues to stir up controversy. One of the most debated points is whether from onset it is a chronic disease or whether it progresses to a chronic form after repeated episodes of acute pancreatitis. Histological studies on patients with alcoholic pancreatitis have shown that the disease is chronic from onset and that alcoholic acute pancreatitis occurs in a pancreas already damaged by chronic lesions. Genetic factors may also play a role in the pathogenesis of alcoholic disease. The incidence of chronic alcoholic pancreatitis seems to have decreased in the last twenty years. Finally, recent therapeutic studies which have shown medical or surgical approaches capable of reducing the pain episodes in chronic pancreatitis patients will be described.
Solid pseudopapillary tumor of the pancreas (SPTP) is a rare pancreatic tumor. Rarely does the tumor have a multicentric location. We report this unusual case of a 24-year-old woman who had 2 large synchronous SPTPs detected incidentally by routine p
Looking for pancreas disease? Find out information about pancreas disease. glandular organ that secretes digestive enzymes and hormones. In humans, the pancreas is a yellowish organ about 7 in. long and 1.5 in. wide. Explanation of pancreas disease
Pancreatic panniculitis is a rare condition, which has only been described in relation with pancreatic diseases up to now. It is characterized by necrotizing subcutaneous inflammation and is thought to be triggered by adipocyte necrosis due to systemic release of pancreatic enzymes with consecutive infiltration of neutrophils. We present the first case of a patient with pancreatic panniculitis caused by pancreatic-type primary acinar cell carcinoma (ACC) of the liver and without underlying pancreatic disease. A 73-year old Caucasian female patient was referred to our department with painful cutaneous nodules persisting for eight weeks and with marked lipasemia (~15000 U/l; normal range |60 U/l). Four weeks prior, several liver lesions had been detected. Empiric treatment with steroids did not show any effect. A biopsy of the skin nodules revealed
Pancreatic panniculitis is a rare condition, which has only been described in relation with pancreatic diseases up to now. It is characterized by necrotizing subcutaneous inflammation and is thought to be triggered by adipocyte necrosis due to systemic release of pancreatic enzymes with consecutive infiltration of neutrophils. We present the first case of a patient with pancreatic panniculitis caused by pancreatic-type primary acinar cell carcinoma (ACC) of the liver and without underlying pancreatic disease. A 73-year old Caucasian female patient was referred to our department with painful cutaneous nodules persisting for eight weeks and with marked lipasemia (~15000 U/l; normal range |60 U/l). Four weeks prior, several liver lesions had been detected. Empiric treatment with steroids did not show any effect. A biopsy of the skin nodules revealed
The role of routine isoamylase determinations in differentiating acute pancreatitis from other causes of an acute abdomen with hyperamylasemia and/or hyperamylasuria was evaluated. Values were analyzed from a control group of 21 patients with acute pancreatitis (group I) and from 100 consecutive patients diagnosed in our emergency department as having an acute abdomen (group II). In group I, 100% of patients had hyperamylasemia, hyperamylasuria, and a P isoamylase fraction greater than 0.75 of the total amylase value. In group II, 50% of patients had hyperamylasemia and/or hyperamylasuria. Of these patients, 44% had a P isoamylase fraction less than 0.75 of the total amylase value, a finding apparently incompatible with a diagnosis of acute pancreatitis as identified by our control group. We conclude that routine isoamylase determinations in patients with an acute abdomen and hyperamylasemia and/or hyperamylasuria allows the differentiation from acute pancreatitis in 44% of cases.
Glutathione S-transferase (GST) isoenzymes of human pancreas were purified, characterized and evaluated for their possible role in the metabolism of ethanol. Human pancreas has at least two GST isoenzymes belonging to the Alpha class (pI 8.8 and 8.1), one belonging to the Mu class (pI 6.4) and one belonging to the Pi class (pI 4.9). During the purification of GSTs from pancreas as well as from heart, liver, lung, brain and muscle, the fatty acid ethyl ester synthase (FAEES) activity was monitored in order to evaluate the role of GSTs in metabolism of ethanol, as suggested in earlier studies. Both t.l.c. and h.p.l.c. were used to identify ethyl oleate in reaction mixtures to monitor FAEES activity. During the purification of GSTs with the use of affinity chromatography on GSH linked to epoxy-activated Sepharose 6B, FAEES and GST activities from each of these tissues segregated independently. Purified GST isoenzymes from these tissues did not exhibit any FAEES activity. Antibodies raised against ...
In Diabetic ketoacidosis when pain abdomen is one of the major clinical finding, serum amylase levels are obtained, to rule out the diagnosis of acute pancreatitis (since the latter too can present with acute pain abdomen). If the serum amylase levels are found high in patients with Diabetic ketoacidosis, it may not be due…
Semin Gastrointest Dis ; Bacterial contamination of pancreatic necrosis: Is CT guided fine needle aspiration helpful in patients with infected necrosis. Acute pancreatitis dietary management MD, Mittal A, van den Heever M, et al, for the Pancreas Network of New Zealand. MRI may also identify early duct disruption that Acute pancreatitis dietary management not seen on CT. The serum amylase level is IU per liter, the serum lipase level is IU per liter, the serum alanine aminotransferase level is Acute pancreatitis dietary management per liter, and the serum lactate dehydrogenase level Acute pancreatitis dietary management IU per liter. For patients with mild acute pancreatitis, nasojejunal feedings can be avoided unless patients are unable to tolerate oral intake for over 1 week. Most of these collections resolve Acute pancreatitis dietary management. Evaluation of severity in patients with acute pancreatitis. Radiol Clin North Am. Generally, however, pancreatic stents are difficult to monitor, ...
Successful pancreas preservation during storage in oxygenated perfluorodecalin (PFD) is mainly related to oxidative ATP generation during storage. Increasing the storage temperature would accelerate this process essential for resuscitation of ischemically damaged pancreatic tissue. The present study aimed at comparing islet isolation outcome from adult pig pancreata preserved in oxygenated PFD by means of a one-layer method during storage on ice or at 20 degrees C. Resected pancreata were intraductally flushed with cold UW solution and promptly processed (n = 6) or stored for 3 h in continuously oxygenated PFD at 4 degrees C (n = 5) or 20 degrees C (n = 7). Prior to digestion-filtration pancreata were intraductally injected with UW supplemented with Serva collagenase NB8 and neutral protease. Islet quality assessment determined viability, glucose stimulation index, mitochondrial activity, intracellular ATP content, and transplant function in diabetic nude mice. Pancreata oxygenated for 3 h at 20 degrees
U.S., March 2 -- ClinicalTrials.gov registry received information related to the study (NCT03064906) titled Insulet Artificial Pancreas Evaluating Meal Performance and Moderate Exercise (IDE2) on Feb. 22. Brief Summary: To assess the safety and performance of the Insulet AP (artificial pancreas) system, using the Omnipod(R) insulin management system, Dexcom G4 Share(R) AP System and personalized model predictive control algorithm in adults with type 1 diabetes consuming high fat meals and undertaking moderate intensity exercise. Study Start Date: Study Type: Interventional Condition: Type 1 Diabetes Mellitus Intervention: Device: Insulet AP (artificial pancreas) System Insulet AP (artificial pancreas) system, using the Omnipod(R) insulin management system, Dexcom G4 Share(R) AP System and personalized model predictive control algorithm. Recruitment Status: Not yet recruiting Sponsor: Insulet Corporation Information provided by (Responsible Party): Insulet Corporation ...
Global Exocrine Pancreatic Insufficiency Market is anticipated to reach US$2.85 Bn by 2023; Prevalence of Exocrine Pancreatic Insufficiency is expected to rise due to increase in number of diabetes patients. Market for exocrine pancreatic insufficiency market is set to expand at a CAGR of 8.3%.
TY - JOUR. T1 - Heterotopic pancreas in omphalomesenteric duct remnant results in persistent umbilical discharge. AU - Park, Eunhyang. AU - Kim, Hyojin. AU - Jung, Whan. AU - Chung, Jin Haeng. PY - 2014. Y1 - 2014. UR - http://www.scopus.com/inward/record.url?scp=84907362254&partnerID=8YFLogxK. U2 - 10.4132/KoreanJPathol.2014.48.4.323. DO - 10.4132/KoreanJPathol.2014.48.4.323. M3 - Article. AN - SCOPUS:84907362254. VL - 48. SP - 323. EP - 326. JO - Korean Journal of Pathology. JF - Korean Journal of Pathology. SN - 1738-1843. IS - 4. ER - ...
Visceral adipose tissue plays a critical role in numerous diseases. While imaging studies often show adipose involvement in abdominal diseases, their outcomes may vary from being a mild self limited illness to one with systemic inflammation and organ failure. We therefore compared the pattern of visceral adipose injury during acute pancreatitis and acute diverticulitis to determine its role in organ failure. Acute pancreatitis-associated adipose tissue had ongoing lipolysis in the absence of adipocyte triglyceride lipase (ATGL). Pancreatic lipase injection into mouse visceral adipose tissue hydrolyzed adipose triglyceride and generated excess non-esterified fatty acids (NEFA), which caused organ failure in the absence of acute pancreatitis. Pancreatic triglyceride lipase (PNLIP) increased in adipose tissue during pancreatitis and entered adipocytes by multiple mechanisms, hydrolyzing adipose triglyceride and generating excessive NEFA. During pancreatitis, obese PNLIP knockout mice, unlike obese ...
Methods are described for controlling exocrine pancreatic function, for reducing the level of amylase in the blood, and for treating pancreatitis in an individual comprising administering to the individual a bone morphogenetic protein (BMP). Methods for identifying candidate molecules for use in treating diabetes are also described.
Deciphering mechanisms of endocrine cell induction, specification and lineage allocation in vivo will provide valuable insights into how the islets of Langerhans are generated. Currently, it is ill defined how endocrine progenitors segregate into different endocrine subtypes during development. Here, we generated a novel neurogenin 3 (Ngn3)-Venus fusion (NVF) reporter mouse line, that closely mirrors the transient endogenous Ngn3 protein expression. To define an in vivo roadmap of endocrinogenesis, we performed single cell RNA sequencing of 36,351 pancreatic epithelial and NVF+ cells during secondary transition. This allowed Ngn3low endocrine progenitors, Ngn3high endocrine precursors, Fev+ endocrine lineage and hormone+ endocrine subtypes to be distinguished and time-resolved, and molecular programs during the step-wise lineage restriction steps to be delineated. Strikingly, we identified 58 novel signature genes that show the same transient expression dynamics as Ngn3 in the 7260 profiled ...
TY - JOUR. T1 - Effect of diet and cold exposure on norepinephrine turnover in pancreas and liver. AU - Young, J. B.. AU - Landsberg, L.. PY - 1979/1/1. Y1 - 1979/1/1. N2 - The potential contribution of the sympathetic nervous system to the regulation of the endocrine pancreas and the liver has previously been studied only in vitro or by indirect in vivo methods. This report describes the adaptation of the [3H]norepinephrine ([3H]NE) turnover technique to rat pancreas and liver as well as the application of this technique to the evaluation of sympathetic activity in these organs during cold exposure, fasting, and overfeeding. Cold exposure (4°C) increased the calculated pancreatic NE turnover rate 83% from 16.6 ± 1.1 ng NE/organ per hr to 30.4 ± 1.9 (95% confidence intervals), whereas hepatic NE turnover rate increased only 25% from 47.0 ± 3.4 to 58.7 ± 3.3. Two days of fasting reduced pancreatic NE turnover rate 70% from 26.8 ± 3.0 ng NE/organ per hr to 8.0 ± 0.8, whereas hepatic NE ...
Endocrine cell differentiation in the pancreas must be tightly controlled to produce appropriate numbers of each endocrine cell type in the islets. Dysregulation of this process can cause severe physiological problems - diabetes, associated with loss of β-cell generation or function, being the most obvious example. One transcription factor known to be involved in β-cell differentiation is Mnx1, mutation of which is associated with neonatal diabetes. By inactivating Mnx1 in either endocrine progenitors or β-cells, Fong Cheng Pan and co-workers have provided insights into the roles of this key regulator in mouse (p. 3637). They find that Mnx1 acts as a lineage specification factor: upon its depletion, β-cells fail to differentiate but δ-cell number is increased. Mnx1 is also needed for β-cell maintenance: mutants show extensive β- to δ-cell transdifferentiation. Intriguingly, the authors identify a small population of escaper β-cells in which Mnx1 has not been depleted, allowing expansion ...
For children with congenital hyperinsulinism (CHI), low blood sugar is caused by cells in the pancreas that release too much insulin. Some children with CHI have these cells throughout their pancreas (called diffuse disease); others have them located in specific areas of the pancreas (called focal disease). Children who have focal disease located in specific areas of the pancreas may be cured with surgery. F-DOPA is a radioactive drug that is picked up by these cells and used for positron emission tomography (or PET), an imaging technique used in nuclear medicine departments. In this study, researchers will validate the efficacy and safety of using PET/CT with F-DOPA in the pre-operative localization of focal disease in children with hyperinsulinism ...
For children with congenital hyperinsulinism (CHI), low blood sugar is caused by cells in the pancreas that release too much insulin. Some children with CHI have these cells throughout their pancreas (called diffuse disease); others have them located in specific areas of the pancreas (called focal disease). Children who have focal disease located in specific areas of the pancreas may be cured with surgery. F-DOPA is a radioactive drug that is picked up by these cells and used for positron emission tomography (or PET), an imaging technique used in nuclear medicine departments. In this study, researchers will validate the efficacy and safety of using PET/CT with F-DOPA in the pre-operative localization of focal disease in children with hyperinsulinism ...
Because successful human islet transplantation requires large quantities of viable islets that must be separated from the highly immunogenic exocrine tissue and because handpicking is too time-consuming and laborious to be clinically relevant, a new approach for solving this problem has been established in rat models. It is based on the principle that magnetic microspheres (MMSs) coupled to lectins with binding specificity for the exocrine tissue portion are trapped in an electromagnetic field, thus providing effluent islets of a high degree of purity. In this study our aim was to adapt this principle to human islet preparations. In this context our prime interest was focused on a lectin suitable for human pancreatic tissue. Of 19 different lectins tested, only 1, Wisteria floribunda agglutinin (WFA), is suitable, as shown by immunofluorescence, MMS-lectin binding, and magnetic separation.. ...
From the APSA 2017 Annual Meeting proceedings. ROBOTIC LONGITUDINAL PANCREATICOJEJUNOSTOMY (PEUSTOW) FOR CHRONIC PANCREATITIS IN AN ADOLESCENT. Anna F. Tyson, MD, MPH, Daniel A. Bambini, MD, John B. Martinie, MD.. Carolinas Medical Center, Charlotte, NC, USA.. Purpose: A fifteen-year-old Hispanic girl presented with a brief history of nausea, vomiting and severe abdominal pain. She had no prior episodes of pain, but reported a remote history of blunt abdominal trauma from a bicycle handle injury. Workup revealed evidence of chronic pancreatitis with diffuse calcifications throughout the pancreas and a dilated, tortuous pancreatic duct. This abstract describes robotic longitudinal pancreaticojejunostomy for management of her disease.. Methods: After thorough and appropriate preoperative workup, the patient underwent robotic longitudinal pancreaticojejunostomy. This was accomplished using three 8mm and two 12mm ports. The gastrocolic omentum was opened using a vessel sealing device, and the ...
TY - CHAP. T1 - Elevation of pancreatic enzymes in bile of PBM. AU - Fujimoto, Takaaki. AU - Ohtsuka, Takao. AU - Nakamura, Masafumi. PY - 2018/6/5. Y1 - 2018/6/5. N2 - Pancreaticobiliary maljunction (PBM) is a congenital anomaly in which the reciprocal reflux between pancreatic and bile juice occurs because the conflu- ence of the pancreatic and bile ducts lies outside of the area of contractile influence of the sphincter of Oddi. The reflux of pancreatic juice into the bile duct and subse- quent elevated pancreatic enzyme level in bile are usually recognized in PBM. Amylase is the most popular pancreatic enzyme for assessment in daily prac- tice, and assessment of amylase level in bile is useful to definitively confirm the presence of PBM, and the mixture of pancreatic and bile juices may produce various physiological and pathological alterations. In PBM, the amylase level in bile in patients with biliary cancers has been reported to be higher than that with benign biliary diseases; however, ...
Plasma pancreatic enzymes and hormones were longitudinally observed after producing partial obstruction of the major pancreatic duct in dogs to study an initial state of chronic pancreatitis or pancreatolithiasis. Fasting plasma immunoreactive cationic trypsin was elevated during the first six months and then decreased in a subgroup with pancreatic opposite at the end of the 12-month period. Similar but less prominent changes were found in fasting plasma immunoreactive pancreatic polypeptide (IRPP). Plasma amylase, glucose, or immunoreactive insulin or glucagon (IRG) show no significant variation. Plasma IRG and IRPP responses to intravenous insulin were reduced in the subgroups with marked pancreatic changes towards the end of the 12-month period. These results suggest that plasma pancreatic enzymes and hormones remain elevated as long as pancreatic damage is mild and then start to decline as the damage progresses in chronic pancreatitis or pancreatolithiasis.
The neuropeptide corticotropin-releasing factor (CRF) makes cameo appearances throughout the body, but its leading role is as the opening act in the stress response, jump-starting the process along the hypothalamus-pituitary-adrenal (HPA) axis. Researchers at the Salk Institute for Biological Studies have found that CRF also plays a part in the pancreas, where it increases insulin secretion and promotes the division of the insulin-producing beta cells.. These findings, which will be published in this weeks edition of the Proceedings of the National Academy of Sciences, may provide new insights into diabetes, particularly type 1, as well as suggest novel targets for drug intervention.. The pancreas is both an exocrine gland, producing enzymes that are secreted into the gut to help digest food, and an endocrine gland, secreting a cocktail of hormones, including insulin, which is manufactured by beta cells that reside in endocrine islets within the "sea" of exocrine tissue.. Plasma glucose ...
Intraductal papillary mucinous neoplasm (IPMN), the most common pancreatic cystic neoplasm, is known to progress to invasive ductal adenocarcinoma. IPMNs commonly harbor activating somatic mutations in GNAS and KRAS, primarily GNAS(R201H) and KRAS(G12D). GNAS encodes the stimulatory G-protein α subunit (Gsα) that mediates a stimulatory signal to adenylyl cyclase to produce cyclic adenosine monophosphate (cAMP), subsequently activating cAMP-dependent protein kinase A. The GNAS(R201H) mutation results in constitutive activation of Gsα. To study the potential role of GNAS in pancreatic tumorigenesis in vivo, we generated lines of transgenic mice in which the transgene consisted of Lox-STOP-Lox (LSL)-GNAS(R201H) under the control of the CAG promoter (Tg(CAG-LSL-GNAS)). These mice were crossed with pancreatic transcription factor 1a (Ptf1a)-Cre mice (Ptf1a(Cre/+)), generating Tg(CAG-LSL-GNAS);Ptf1a(Cre/+) mice. This mouse line showed elevated cAMP levels, small dilated tubular complex formation, ...
Robert Sherwin, M.D., Yale University, who chaired the panel, says he "believes, with certain safeguards, artificial pancreas systems can be safely tested in real world settings." Other experts speaking at the workshop included David Nathan, Director, Clinical Research Center and Diabetes Center at Massachusetts General Hospital and Professor of Medicine, Harvard Medical School; and William Tamborlane, Professor and Chief of Pediatric Endocrinology and Diabetes, Yale University School of Medicine.. "The incidence of type 1 diabetes is on the rise. Todays tools to manage the disease are insufficient. We have the technology at our disposal to make an artificial pancreas work. Now its time to move forward quickly to define the regulatory pathway so final studies can be completed and better technologies can be made available to adults and children struggling with this difficult disease," added Mr. Brewer. Development of the artificial pancreas device began in 2005 to help individuals living with ...
Fetal rat pancreatic cells were isolated from pancreatic primordia on days 12-14 of pregnancy and cultured for 48 h in the presence of 5 mmol/l glucose. Insulin accumulation in the medium over the next 24 h was measured. Cultured cells from day 12 fetuses secreted about 1 fmol insulin per pancreas in response to 5 or 15 mmol/l glucose irrespective of whether 1 mmol/l tolbutamide, 400 mumol/l diazoxide, 5 mmol/l theophylline or 10 mmol/l mannoheptulose was present. In contrast, insulin released from day 13 cultured cells increased significantly from 3.0 +/- 0.6 to 6.2 +/- 2.2 fmol per pancreas, when the glucose concentration was raised. Tolbutamide increased, diazoxide and mannoheptulose decreased and theophylline had no effect on insulin release. Even more pronounced effects were found on insulin release from day 14 cultured cells, in which theophylline also increased the release. In addition, insulin release from cells from pregnancy day 14 was 75 +/- 16 amol/min per pancreas when the cells ...
1. Introduction. Medical treatment of chronic pancreatitis is based on the three main characteristics of the disease, pain and exocrine and endocrine insufficiency. Pain is the leading symptom of chronic pancreatitis. Patients may suffer from continuous pain or relapsing pain in parallel with relapses of the chronic inflammatory process or complications. Pain may decrease over time due to what is called "burn out" disease. Treatment of pain should be based on its pathogenesis. However, in many instances the pathogenesis of pain remains unclear. Pain may be due to an inflammatory mass of the pancreatic head which doesn`t resolve over time and is best treated by resectional surgery; e.g. duodenum preserving pancreatic head resection. Pain due to obstruction of the main pancreatic duct by calcified protein plaques may be treated by ESWL (extracorporal shock wave lithotripsy) with or without endoscopic placement of a stent into the pancreatic duct. These options are discussed in other chapters. ...
TY - JOUR. T1 - Clinical presentation of mucin-secreting tumors of the pancreas. AU - Tibayan, Frederick (Fred). AU - Vierra, Mark. AU - Mindelzun, Bob. AU - Tsang, Don. AU - McClenathan, James. AU - Young, Harvey. AU - Trueblood, H. Ward. PY - 2000/5/15. Y1 - 2000/5/15. N2 - Background: Pancreatitis and jaundice secondary to ductal obstruction are common in intraductal papillary mucinous tumors (IPMT) of the pancreas. However, the incidence and severity of the complications of obstruction are not well documented. The aim of the study was to investigate the clinical presentation and outcome of 10 patients with IPMT. Methods: All cases of IPMT diagnosed between 1994 and 1999 were reviewed. Results: Four of the 10 patients developed severe acute illness with systemic complications resulting from ductal obstruction. Three suffered acute cholangitis with sepsis, and 1 developed necrotizing pancreatitis and ARDS. There was 1 postoperative death in a patient with adenocarcinoma. All other patients are ...
A slightly dilated pancreatic duct (top) is noted which ends abruptly due to a hypovascular mass in the body of the pancreas (bottom). Mild exudate is present in the left retroperitoneal space. Patient underwent surgery and pancreatic adenocarcinoma was confirmed at pathology.. Necrotizing pancreatitis caused by pancreatic adenocarcinoma is rarely reported and notoriously difficult to diagnose and treat, as the extensive peripancreatic changes associated with necrotizing pancreatitis would likely render curative resection impossible in the majority of cases (121). Pancreatic adenocarcinoma as the cause of pancreatitis is surrounded by pitfalls in clinical presentation and diagnostic imaging features leading to delays in correct diagnosis and appropriate treatment (7, 30, 72, 106). Often, the diagnosis of an occult pancreatic adenocarcinoma is masked by the clinical presentation of signs and symptoms of acute pancreatitis. Also, on imaging, features of the inflammatory process may hamper the ...
Aberrant Fhit expression characterizes a large proportion of primary pancreatic ductal adenocarcinomas (PDACs), but fragmentary information is available on Fhit expression during the phenotypic changes of pancreatic ductal epithelium during multistep transformation. We assessed Fhit expression by immunohistochemistry in two different multistep pancreatic carcinogenic processes: pancreatic intraepithelial neoplasia (PanIN) and intraductal papillary mucinous neoplasia (IPMN). We considered 105 surgically treated PDACs/IPMNs and selected 30 samples of non-neoplastic pancreatic parenchyma, 50 PanIN lesions, 30 IPMNs, 15 IPMNs with associated invasive carcinoma, and 60 adenocarcinomas. Normal pancreatic ducts and surrounding acinar cells consistently showed moderate to strong Fhit immunoreactivity. Significant down-regulation of Fhit expression was observed in association with increasing severity of dysplastia/neoplastia in both carcinogenic processes. This was further confirmed by studying multiple ...
OBJECTIVE-Diabetes results from a deficiency of functional β-cells due to both an increase in β-cell death and an inhibition of β-cell replication. The molecular mechanisms responsible for these effects in susceptible individuals are mostly unknown. The objective of this study was to determine whether a gene critical for cell division and cell survival in cancer cells, survivin, might also be important for β-cells.. RESEARCH DESIGN AND METHODS-We generated mice harboring a conditional deletion of survivin in pancreatic endocrine cells using mice with a Pax-6-Cre transgene promoter construct driving tissue-specific expression of Cre-recombinase in these cells. We performed metabolic studies and immunohistochemical analyses to determine the effects of a mono- and biallelic deletion of survivin.. RESULTS-Selective deletion of survivin in pancreatic endocrine cells in the mouse had no discernible effects during embryogenesis but was associated with striking decreases in β-cell number after ...
GLP-1(28-36)amide, the Glucagon-like peptide-1 metabolite: friend, foe, or pharmacological folly? Meng-Wong Taing,1,2 Felicity J Rose,1,3 Jonathan P Whitehead11Metabolic Medicine, Mater Research Institute, University of Queensland, 2School of Pharmacy, University of Queensland, Brisbane, QLD, Australia; 3University of Queensland Diamantina Institute, Translational Research Institute, Brisbane, QLD, AustraliaAbstract: The glucagon-like peptide-1 (GLP-1) axis has emerged as a major therapeutic target for the treatment of type 2 diabetes. GLP-1 mediates its key insulinotropic effects via a G-protein coupled receptor expressed on β-cells and other pancreatic cell types. The insulinotropic activity of GLP-1 is terminated via enzymatic cleavage by dipeptidyl peptidase-4. Until recently, GLP-1-derived metabolites were generally considered metabolically inactive; however, accumulating evidence indicates some have biological activity that may contribute to the pleiotropic effects of GLP-1 independent of the
Lets discuss Ibuprofen for pancreatitis. I take it for acute pancreatitis, only acute pancreatitis. If you have chronic pancreatitis you may want to try taking Ibuprofen for pancreatitis daily but ask your doctor if it is safe to take with your current drug regimen. Ibuprofen for pancreatitis is simply common sense but it is NOT a safe drug. Actually there are no safe drugs. A Big Pharma company could take something…. ...
Over the years, pancreatectomy has been the primary treatment for chronic pancreatitis. Since the 1970s, total pancreatectomy has been shown to be effective at relieving pain that was experienced due to chronic pancreatitis. However, total pancreatectomy alone has the significant side effect of post-surgical diabetes. To treat post-pancreatectomy diabetes, total pancreatectomy was combined with islet autotransplantation (TP-IAT), which resulted in a much lower mortality rate compared to total pancreatectomy alone. Such operations require an expertise in islet isolation techniques, and thus this procedure is not widely performed.
Looking for online definition of insulinase in the Medical Dictionary? insulinase explanation free. Gestational diabetes is a form of High blood sugar in the mother can cause the in 2016 he launched an INITIATIVE to give back to the world by partnering with Silicon Valley Eye Physicians offers expert eye care and optemetry services for the Sunneyvale and Santa Clara CA areas as well as optical and contact lens stores. Candied Popcorn Recipe Jello reverse Diabetes - Tips For Reversing Diabetes. all mobile porno for pyros pets albumin levels normal range for blood. Transplants of insulin-producing pancreas cells are a long hoped-for treatment for type 1 diabetes. This lining is called the retina.. Find great deals on eBay for diabetic cakes and diabetic biscuits. Guaranteed 2 Day Delivery in the US. To search for retreats camps & recreational programs by a particular Juvenile Diabetes Arthritis - Juvenile Asthma Not the thoracic cavity.. Australian Diabetes Educators what is a good white wine for ...
CAPELLA, C., EUSEBI, V., MANN, B. and AZZOPARDI, J. G. (1980), Endocrine differentiation in mucoid carcinoma of the breast. Histopathology, 4: 613-630. doi: 10.1111/j.1365-2559.1980.tb02957.x ...
Aid, Antibodies, Biology, Endocrine Cells, Endoderm, Mice, Insulin, Cell, Cells, Adults, Manganese, Oxygen, Superoxide, and Administration
The attitude of the cooler-than-cool couple in front of us in line for Amon Tobin, the Brazilian electronic musician/mad scientist, pretty much summed up the evening for me. Sure, I expected hipsters galore at a show like this -- while Ive never really met any other Amon Tobin fans,...

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry | ProtocolAn Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry | Protocol

The in vitro micronucleus assay is a well-established method for evaluating genotoxicity and cytotoxicity but scoring the assay ... In light of these limitations, Multispectral Imaging Flow Cytometry (MIFC) is an ideal system to perform the MN assay since it ... An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry. Matthew A. Rodrigues ... Rodrigues, M. A. An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry. J. ...
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Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry | ProtocolQuantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry | Protocol

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry… ... Neubert, H., Gale, J., Muirhead, D. Online high-flow peptide immunoaffinity enrichment and nanoflow LC-MS/MS: assay development ... Add stable isotope standard (multiple standards are added if performing a multiplexed assay, typically this is about 10 μL ... Using Retinal Imaging to Study Dementia. Published 11/06/2017. Regioselective O-Glycosylation of Nucleosides via the Temporary ...
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Rapid LC/MS/MS-Based Methods For Opiates & BenzodiazepinesRapid LC/MS/MS-Based Methods For Opiates & Benzodiazepines

... work is the first demonstration of fully automated method for performing the in vitro MN assay on an imaging flow cytometry ... adaptation of the in vitro MN assay to an imaging flow cytometry-based method will be described. Using the ISX Mark II imaging ... Automation of the in vitro micronucleus assay using imaging flow cytometry Recorded: Oct 24 2017 57 mins Matthew. A Rodrigues, ... Characterization of sub-visible particles with Amnis imaging flow cytometry Recorded: Nov 30 2017 43 mins Christine Probst, ...
more infohttps://www.brighttalk.com/webcast/1238/28345

Late Stage Functionalization of Drug-Like Molecules Session 2Late Stage Functionalization of Drug-Like Molecules Session 2

... work is the first demonstration of fully automated method for performing the in vitro MN assay on an imaging flow cytometry ... adaptation of the in vitro MN assay to an imaging flow cytometry-based method will be described. Using the ISX Mark II imaging ... Automation of the in vitro micronucleus assay using imaging flow cytometry Recorded: Oct 24 2017 57 mins Matthew. A Rodrigues, ... Characterization of sub-visible particles with Amnis imaging flow cytometry Nov 30 2017 4:00 pm UTC 75 mins Christine Probst, ...
more infohttps://www.brighttalk.com/webcast/1238/143587

MK2 inhibitory peptide delivered in nanopolyplexes prevents vascular graft intimal hyperplasia | Science Translational MedicineMK2 inhibitory peptide delivered in nanopolyplexes prevents vascular graft intimal hyperplasia | Science Translational Medicine

... and characterized NPs and performed in vitro assays, flow cytometry, organ culture experiments, image analysis, histological ... Funding: Confocal imaging was performed in part through the use of the Vanderbilt University Medical Center Cell Imaging Shared ... K.V.K. performed fluorescence confocal microscopy. J.D. performed cell migration experiments. M.J.O and I.V. performed rabbit ... A) Representative flow cytometry histograms of MK2i internalization when delivered as a free peptide or via NPs. (B) Red blood ...
more infohttps://stm.sciencemag.org/content/7/291/291ra95

Formin-generated actomyosin arcs propel T cell receptor microcluster movement at the immune synapse | JCBFormin-generated actomyosin arcs propel T cell receptor microcluster movement at the immune synapse | JCB

Amnis ImageStream flow cytometry assay. Imaging flow cytometry was performed as previously described (Markey et al., 2015). In ... H) Imaging flow cytometry of Jurkat T-B cell conjugates fixed, immunostained, and analyzed on a flow cytometer for background- ... TIRF-SIM imaging was performed as described previously (Beach et al., 2014; Li et al., 2015). In brief, images were acquired ... Adhesion conjugate assay. The adhesion conjugate assay was performed as previously described (Cannons et al., 2010; Zhao et al ...
more infohttp://jcb.rupress.org/content/215/3/383

Amnis - Frequently Asked QuestionsAmnis - Frequently Asked Questions

The powerful combination of quantitative image analysis and flow cytometry in a single platform creates exceptional new ... instruments are designed to perform any standard flow cytometry assay as well as a wide range of quantitative imaging assays. ... The FlowSight, when equipped with the Quantitative Imaging option, can also perform many of the quantitative image-based assays ... Most sample preparation protocols for flow cytometry, including those for adherent cells, can be adapted to imaging flow ...
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Cell Analysis
							
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... tips and troubleshooting advice on current topics like cell flow cytometry, cell counts and more. ... work is the first demonstration of fully automated method for performing the in vitro MN assay on an imaging flow cytometry ... imaging flow cytometry and more. Automation of the In Vitro Micronucleus Assay using Imaging Flow Cytometry. ... How imaging flow cytometry allows simultaneous phenotypic and functional studies. *How imaging flow cytometry advances research ...
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Cell Analysis - Webinars | Support | MerckCell Analysis - Webinars | Support | Merck

... tips and troubleshooting advice on current topics like cell flow cytometry, cell counts and more. ... work is the first demonstration of fully automated method for performing the in vitro MN assay on an imaging flow cytometry ... imaging flow cytometry and more. Automation of the In Vitro Micronucleus Assay using Imaging Flow Cytometry. ... How imaging flow cytometry allows simultaneous phenotypic and functional studies. *How imaging flow cytometry advances research ...
more infohttp://www.merckmillipore.com/DE/de/support/support-library-webinars-videos-trainings/webinars/life-science-research/Cell-Analysis/5Ieb.qB.ZpsAAAFGeL0PPoZt,nav

Plus itPlus it

Effect of selected positive hits on ABCG2 function shown by flow cytometry analysis of the BODIPY-prazosin dye uptake assay. ... d-luciferin was injected i.p. into mice at 150 mg/kg, and imaging was performed every few minutes for ,1 h. ABCG2 inhibitor was ... and analyzed with flow cytometry. Analyses were performed with FACScan (Becton Dickinson) with an excitation wavelength of 488 ... Identification of Inhibitors of ABCG2 by a Bioluminescence Imaging-Based High-Throughput Assay. Yimao Zhang, Youngjoo Byun, ...
more infohttp://cancerres.aacrjournals.org/content/69/14/5867

Post-doctoral Fellow - Department of Microbiology  job with THE UNIVERSITY OF HONG KONG | 73094Post-doctoral Fellow - Department of Microbiology job with THE UNIVERSITY OF HONG KONG | 73094

He/She will need to design and perform experiments, which involve animal immunization, immune assays, flow cytometry, cell ... imaging, deep sequencing and data analysis, etc. Enquiries about the post can be directed to the AIDS Institute at [email protected] ...
more infohttps://www.timeshighereducation.com/unijobs/listing/73094/post-doctoral-fellow-department-of-microbiology-/

FluorescenceFluorescence

The Cellometer Spectrum Image Cytometer performs cell-based assays in minutes. The system has user-changeable fluorescent ... optics modules and provides flow results without flow cytometry.. By Polly Mitchell,2018-11-06T18:33:21+00:00October 22nd, 2018 ... Bright field and Fluorescent Image Analysis for Screening Applications using Celigo Gallery Bright field and Fluorescent Image ... Categories: Cellometer Product News, Latest News,Tags: cell-based assays, Cellometer Spectrum, Fluorescence, functional assays ...
more infohttps://www.nexcelom.com/tag/fluorescence/

Latest NewsLatest News

The Cellometer Spectrum Image Cytometer performs cell-based assays in minutes. The system has user-changeable fluorescent ... optics modules and provides flow results without flow cytometry.. By Polly Mitchell,2018-11-06T18:33:21+00:00October 22nd, 2018 ... Directly image and count calcein AM-labeled target cells in a 96-well plate using Celigo image cytometer. Concurrent imaging ... Video Workflow-at-a-Glance: Seahorse™ Normalization Assay. Celigo Image Cytometer provides a fast, effective, and cell stress- ...
more infohttps://www.nexcelom.com/category/latest-news/

Development of Mature and Functional Human Myeloid Subsets in Hematopoietic Stem Cell-Engrafted NOD/SCID/IL2rγKO Mice | The...Development of Mature and Functional Human Myeloid Subsets in Hematopoietic Stem Cell-Engrafted NOD/SCID/IL2rγKO Mice | The...

... we performed in vitro phagocytosis assay using yellow-green fluorescent beads by flow cytometry and confocal microscopy imaging ... Flow cytometry and confocal imaging demonstrated that human bone marrow monocytes and alveolar macrophages in the recipients ... A and B) Expression of TLR2 and TLR4 in neutrophils, monocytes, BDCA-1+ DCs, and BDCA-3+ DCs was analyzed by flow cytometry. (C ... A) Representative flow cytometry contour plots demonstrating differentiation of human HLA-DR− granulocytes and HLA-DR+ APCs in ...
more infohttp://www.jimmunol.org/content/188/12/6145?ijkey=81f9c302b61463b09697a2b564ac486a90e2ba81&keytype2=tf_ipsecsha

Powerpoint Slides Cartoons - Boring Seminar - Best Science Cartoons | VADLO Biomedical Sciences Search EnginePowerpoint Slides Cartoons - Boring Seminar - Best Science Cartoons | VADLO Biomedical Sciences Search Engine

... flow cytometry, gel electrophoresis, performing immunofluorescence staining, confocal imaging and optical microscopy, ... kinase assays and signaling pathways, making stem cell culture media, sera, generating chemical buffers, and using transfection ...
more infohttp://vadlo.com/cartoons.php?id=191

VADLO - Life Sciences Search EngineVADLO - Life Sciences Search Engine

... flow cytometry, gel electrophoresis, performing immunofluorescence staining, confocal imaging and optical microscopy, ... kinase assays and signaling pathways, making stem cell culture media, sera, generating chemical buffers, and using transfection ...
more infohttp://vadlo.com

Plus itPlus it

... and incubated single cell suspensions with glucose prior to flow cytometry (14) or performed immunohistochemistry with ... We used ApopTag Kit for TUNEL assays (Millipore) and stained nuclei with DAPI or DRAQ5 (Cell Signaling Technology). Image ... 5C). Next, we examined β-cell replication by flow cytometry. We detected a threefold increase of Ki67+/insulin+ cells in islets ... 1H). Analysis of exocrine cell proliferation by flow cytometry of Ki67+/amylase+ cells and Ki67+ immunohistochemistry showed an ...
more infohttp://diabetes.diabetesjournals.org/content/64/8/2868

Flow Cytometry / Cell Counting > Cell Counter  Laboratory Product Directory and Equipment Reviews on...Flow Cytometry / Cell Counting > Cell Counter Laboratory Product Directory and Equipment Reviews on...

Read reviews and compare manufacturers of Flow Cytometry / Cell Counting > Cell Counter products in the SelectScience products ... The NC-250™ can perform high speed cell count and viability determination, a unique 5-minute cell cycle assay a... Read more... ... The NucleoCounter® NC-200™ is a high precision automated cell counter utilizing state-of-the-art image cytometry. With user ... The NucleoCounter® NC-250™ is a cell analyzer utilizing fluorescence imaging to characterize cell properties. ...
more infohttp://www.selectscience.net/flow-cytometry-+-cell-counting/product-directory/cell-counter/?catID=5038&u=0FC5D6DF-2B08-4228-B957-C4F63A1D2E6D

Flow Cytometry / Cell Counting > Cell Counter  Laboratory Product Directory and Equipment Reviews on...Flow Cytometry / Cell Counting > Cell Counter Laboratory Product Directory and Equipment Reviews on...

Read reviews and compare manufacturers of Flow Cytometry / Cell Counting > Cell Counter products in the SelectScience products ... The NC-250™ can perform high speed cell count and viability determination, a unique 5-minute cell cycle assay a... Read more... ... The NucleoCounter® NC-200™ is a high precision automated cell counter utilizing state-of-the-art image cytometry. With user ... Roche Innovatis Cedex HiRes is the newest generation of image-based Cedex Cell Counter and Analyzer systems for automated cell ...
more infohttp://www.selectscience.net/flow-cytometry-+-cell-counting/product-directory/cell-counter/?catID=5038&u=BC53F2CC-52FD-4A49-A458-C5CE3C886A48

Receptor determinants of zoonotic transmission of New World hemorrhagic fever arenaviruses | PNASReceptor determinants of zoonotic transmission of New World hemorrhagic fever arenaviruses | PNAS

... cells were imaged by fluorescence microscopy and detached with trypsin for GFP fluorescence analysis by flow cytometry. ... Each assay was performed in duplicate and repeated three to five times. ... C) In parallel, cell surface binding of MACV GP1Δ-Fc was determined by flow cytometry, as in Fig. 1 B. Mean fluorescence values ... B) In parallel, cell surface binding of MACV GP1Δ-Fc was determined by flow cytometry. Mean fluorescence values were normalized ...
more infohttps://www.pnas.org/content/105/7/2664?ijkey=bf4cc233727c2057a5358f6879445a148a72c8f2&keytype2=tf_ipsecsha

Gas1 up-regulation is inducible and contributes to cell apoptosis in reactive astrocytes in the substantia nigra of LPS and...Gas1 up-regulation is inducible and contributes to cell apoptosis in reactive astrocytes in the substantia nigra of LPS and...

... flow cytometry, and siRNA knockdown methods. Gas1 expressions were significantly elevated in the majority of the reactive ... Cell cultures were performed to analyze Gas1 functions in the viability and apoptosis of reactive astrocytes and SH-SY5Y cells ... a CCK-8 assay, LDH release, flow cytometry assay, TUNEL staining, and GAFP/Ki-67 and GFAP/BrdU double-labeling were performed. ... A-A", Gas1 single, GFAP single, and Gas1/GFAP merged images in the nigral astrocytes of LPS rat model-3w; B-B", Gas1 single, TH ...
more infohttps://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-016-0643-2

Cell Analysis
							
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... tips and troubleshooting advice on current topics like cell flow cytometry, cell counts and more. ... work is the first demonstration of fully automated method for performing the in vitro MN assay on an imaging flow cytometry ... imaging flow cytometry and more. Automation of the In Vitro Micronucleus Assay using Imaging Flow Cytometry. ... How imaging flow cytometry allows simultaneous phenotypic and functional studies. *How imaging flow cytometry advances research ...
more infohttp://www.emdmillipore.com/CA/en/support/support-library-webinars-videos-trainings/webinars/life-science-research/Cell-Analysis/5Ieb.qB.ZpsAAAFGeL0PPoZt,nav

Lactobacillus paracasei feeding improves the control of secondary experimental meningococcal infection in flu-infected mice |...Lactobacillus paracasei feeding improves the control of secondary experimental meningococcal infection in flu-infected mice |...

... and the homogenates used to perform the cytokine assay and flow cytometry analysis as previously described [15]. ... Analysis and acquisition were performed using Living Image 4.3.1 software (Xenogen Corp.). Images were acquired using a 1-min ... Antibodies and flow cytometry. The second part of whole-lung homogenates were used to prepare single-cell suspensions were ... Figure S1. The figure describes the flow cytometry analysis of immune cells in the lungs: The used antibodies and the gating ...
more infohttps://bmcinfectdis.biomedcentral.com/articles/10.1186/s12879-018-3086-9

Unmasking Cell Replication-Reagents for Analyzing Cell Cycle and Replication in Mammalian Cells | Thermo Fisher Scientific - AUUnmasking Cell Replication-Reagents for Analyzing Cell Cycle and Replication in Mammalian Cells | Thermo Fisher Scientific - AU

Click-iT® EdU assays for imaging and flow cytometry platforms are useful for detecting cells transitioning into the DNA ... Follow DNA Synthesis With Click-iT® EdU Assays The Click‑iT® EdU imaging and flow cytometry assays are novel alternatives to ... allows cell cycle analysis to be performed in live cells and is particularly powerful when combined with time-lapse imaging. ... For fixed-cell imaging and flow cytometry assays, Click-iT® EdU labeling allows for efficient detection of the incorporated EdU ...
more infohttps://www.thermofisher.com/au/en/home/references/newsletters-and-journals/bioprobes-journal-of-cell-biology-applications/bioprobes-issues-2012/bioprobes-67-june-2012/cell-replication-cell-cycle-reagents.html

Near infrared imaging of epidermal growth factor receptor positive xenografts in mice with domain I/II specific antibody...Near infrared imaging of epidermal growth factor receptor positive xenografts in mice with domain I/II specific antibody...

Flow cytometry binding assay. In vitro binding studies of IRDye800CW-labeled 8708 and 8709 antibody fragments were performed ... W.B. designed and performed experiments (labeling, flow cytometry, cell culture, xenografts and imaging), analyzed data and ... Click on the image to enlarge.). Figure 3 8708 and 8709 fragment flow cytometry. 8708 and 8709 fragments were titrated with A- ... PET imaging of her1-expressing xenografts in mice with 86Y-CHX-A-dtpa-cetuximab. Eur J Nucl Med Mol Imaging. 2010;37:1368-76 ...
more infohttp://thno.org/v09p0974.htm
  • However, these methods have their own inherent limitations such as inability to visualize the cytoplasm of the cell and the lack of visual MN verification or image data storage with flow cytometry. (jove.com)
  • The FlowSight operates at a fixed 20X magnification, can accept up to four excitation lasers, and is optimized for standard flow cytometry assays with visual verification of all events and populations for improved gating and artifact detection. (amnis.com)
  • To search for clinically useful ABCG2 inhibitors, a bioluminescence imaging (BLI)-based assay was developed to allow high-throughput compound screening. (aacrjournals.org)
  • Applications include the development and implementation of assays for phenotypic hit identification screens, but in particular to apply disease-relevant cell-based assays for compound characterisation during H2L and lead optimisation. (evotec.com)
  • This assay exploits our finding that d -luciferin, the substrate of firefly luciferase (fLuc), is a specific substrate of ABCG2, and ABCG2 inhibitors block the export of d -luciferin and enhance bioluminescence signal by increasing intracellular d -luciferin concentrations. (aacrjournals.org)
  • The results are then visualized and quantified using a microscope that is capable of exciting the dye and recording images. (wikipedia.org)
  • Beyond biology and drug discovery know-how, Evotec has access to a world-class portfolio of assay technologies for biochemical and cellular assays that have shown to be drivers for the success of our partner's projects. (evotec.com)
  • With a plethora of biochemical, biophysical and cellular assay technologies, Evotec scientists will combine many readouts to reveal the true mechanism of action of compounds to aid the selection and optimisation of the best chemical starting points. (evotec.com)
  • Evotec is a world leader in performing cellular and tissue-based imaging assays. (evotec.com)
  • Imaging-based assays are enabling as they allow to combine complex cellular models with disease-relevant assay read-outs. (evotec.com)
  • Automated image based screening permits the identification of small compounds altering cellular phenotypes and is of interest for the discovery of new pharmaceuticals and new cell biological tools for modifying cell function. (wikipedia.org)
  • Molecular-targeted imaging probes are used to identify tumors, define tumor markers and margins, and monitor tumor response to therapy. (thno.org)
  • This study describes the development of a BLI-based high-throughput assay to screen for ABCG2 inhibitors. (aacrjournals.org)
  • IRDye800CW-labeled 8708 (scFv) 2 and 8709 scFv-Fc imaging probes showed high levels of accumulation and good retention in EGFR-positive xenografts, with peak accumulation occurring at 24 and 48 hours post injection, respectively. (thno.org)
  • For each target, approach and philosophy, Evotec utilises the most appropriate technology coupled to disease-relevant assay design. (evotec.com)
  • Separate but compatible signal amplification systems enable the multiplex assay (up to two targets per assay). (wikipedia.org)
  • We expressed, purified, and labeled the IgG and fragments with IRDye800CW and used them to image EGFR-positive and -negative xenografts in CD-1 nude mice. (thno.org)
  • The imaging capabilities of the ImageStreamX Mark II can be further enhanced with the MultiMag option, which adds 60X and 20X objectives, as well as the Extended Depth of Field (EDF) option, neither of which are available for the FlowSight. (amnis.com)
  • IRDye800CW-labeled 8708 (scFv) 2 and 8709 scFv-Fc had desirable binding affinities, clearance times, and tumor accumulation to be used for imaging in combination with current EGFR targeted therapies. (thno.org)
  • EGFR is widely studied as a therapeutic target and more recently as an imaging target. (thno.org)