A collection of cloned peptides, or chemically synthesized peptides, frequently consisting of all possible combinations of amino acids making up an n-amino acid peptide.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell type. It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences.
A technology, in which sets of reactions for solution or solid-phase synthesis, is used to create molecular libraries for analysis of compounds on a large scale.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The structure of one molecule that imitates or simulates the structure of a different molecule.
Temperate bacteriophage of the genus INOVIRUS which infects enterobacteria, especially E. coli. It is a filamentous phage consisting of single-stranded DNA and is circularly permuted.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
Peptides composed of between two and twelve amino acids.
Collections of systematically acquired and organized information resources, and usually providing assistance to users. (ERIC Thesaurus, http://www.eric.ed.gov/ accessed 2/1/2008)
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Viruses whose hosts are bacterial cells.
Methods used for studying the interactions of antibodies with specific regions of protein antigens. Important applications of epitope mapping are found within the area of immunochemistry.
A genus of filamentous bacteriophages of the family INOVIRIDAE. Organisms of this genus infect enterobacteria, PSEUDOMONAS; VIBRIO; and XANTHOMONAS.
Sites on an antigen that interact with specific antibodies.
Peptides whose amino and carboxy ends are linked together with a peptide bond forming a circular chain. Some of them are ANTI-INFECTIVE AGENTS. Some of them are biosynthesized non-ribosomally (PEPTIDE BIOSYNTHESIS, NON-RIBOSOMAL).
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
A 60-kDa extracellular protein of Streptomyces avidinii with four high-affinity biotin binding sites. Unlike AVIDIN, streptavidin has a near neutral isoelectric point and is free of carbohydrate side chains.
Antigenic determinants recognized and bound by the B-cell receptor. Epitopes recognized by the B-cell receptor are located on the surface of the antigen.
Services offered to the library user. They include reference and circulation.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. A commonly observed structure may be composed of a CONSERVED SEQUENCE which can be represented by a CONSENSUS SEQUENCE.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Small cationic peptides that are an important component, in most species, of early innate and induced defenses against invading microbes. In animals they are found on mucosal surfaces, within phagocytic granules, and on the surface of the body. They are also found in insects and plants. Among others, this group includes the DEFENSINS, protegrins, tachyplesins, and thionins. They displace DIVALENT CATIONS from phosphate groups of MEMBRANE LIPIDS leading to disruption of the membrane.
Information centers primarily serving the needs of hospital medical staff and sometimes also providing patient education and other services.
A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)
Antibodies produced by a single clone of cells.
The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
A form of GENE LIBRARY containing the complete DNA sequences present in the genome of a given organism. It contrasts with a cDNA library which contains only sequences utilized in protein coding (lacking introns).
An agency of the NATIONAL INSTITUTES OF HEALTH concerned with overall planning, promoting, and administering programs pertaining to advancement of medical and related sciences. Major activities of this institute include the collection, dissemination, and exchange of information important to the progress of medicine and health, research in medical informatics and support for medical library development.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Proteins prepared by recombinant DNA technology.
Collection and analysis of data pertaining to operations of a particular library, library system, or group of independent libraries, with recommendations for improvement and/or ordered plans for further development.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
Analysis of PEPTIDES that are generated from the digestion or fragmentation of a protein or mixture of PROTEINS, by ELECTROPHORESIS; CHROMATOGRAPHY; or MASS SPECTROMETRY. The resulting peptide fingerprints are analyzed for a variety of purposes including the identification of the proteins in a sample, GENETIC POLYMORPHISMS, patterns of gene expression, and patterns diagnostic for diseases.
Techniques utilizing cells that express RECOMBINANT FUSION PROTEINS engineered to translocate through the CELL MEMBRANE and remain attached to the outside of the cell.
A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.
Planning, organizing, staffing, direction, and control of libraries.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Established cell cultures that have the potential to propagate indefinitely.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
The rate dynamics in chemical or physical systems.
Large collections of small molecules (molecular weight about 600 or less), of similar or diverse nature which are used for high-throughput screening analysis of the gene function, protein interaction, cellular processing, biochemical pathways, or other chemical interactions.
The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to alpha helices, beta strands (which align to form beta sheets) or other types of coils. This is the first folding level of protein conformation.
Rapid methods of measuring the effects of an agent in a biological or chemical assay. The assay usually involves some form of automation or a way to conduct multiple assays at the same time using sample arrays.
The techniques used to produce molecules exhibiting properties that conform to the demands of the experimenter. These techniques combine methods of generating structural changes with methods of selection. They are also used to examine proposed mechanisms of evolution under in vitro selection conditions.
The molecular designing of drugs for specific purposes (such as DNA-binding, enzyme inhibition, anti-cancer efficacy, etc.) based on knowledge of molecular properties such as activity of functional groups, molecular geometry, and electronic structure, and also on information cataloged on analogous molecules. Drug design is generally computer-assisted molecular modeling and does not include pharmacokinetics, dosage analysis, or drug administration analysis.
Procedures by which protein structure and function are changed or created in vitro by altering existing or synthesizing new structural genes that direct the synthesis of proteins with sought-after properties. Such procedures may include the design of MOLECULAR MODELS of proteins using COMPUTER GRAPHICS or other molecular modeling techniques; site-specific mutagenesis (MUTAGENESIS, SITE-SPECIFIC) of existing genes; and DIRECTED MOLECULAR EVOLUTION techniques to create new genes.
Study of the principles and practices of library administration and services.
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
Local surface sites on antibodies which react with antigen determinant sites on antigens (EPITOPES.) They are formed from parts of the variable regions of FAB FRAGMENTS.
Libraries in which a major proportion of the resources are available in machine-readable format, rather than on paper or MICROFORM.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Antigenic determinants recognized and bound by the T-cell receptor. Epitopes recognized by the T-cell receptor are often located in the inner, unexposed side of the antigen, and become accessible to the T-cell receptors after proteolytic processing of the antigen.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
A PEPTIDE that is secreted by the BRAIN and the HEART ATRIA, stored mainly in cardiac ventricular MYOCARDIUM. It can cause NATRIURESIS; DIURESIS; VASODILATION; and inhibits secretion of RENIN and ALDOSTERONE. It improves heart function. It contains 32 AMINO ACIDS.
A biosensing technique in which biomolecules capable of binding to specific analytes or ligands are first immobilized on one side of a metallic film. Light is then focused on the opposite side of the film to excite the surface plasmons, that is, the oscillations of free electrons propagating along the film's surface. The refractive index of light reflecting off this surface is measured. When the immobilized biomolecules are bound by their ligands, an alteration in surface plasmons on the opposite side of the film is created which is directly proportional to the change in bound, or adsorbed, mass. Binding is measured by changes in the refractive index. The technique is used to study biomolecular interactions, such as antigen-antibody binding.
A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
A theoretical representative nucleotide or amino acid sequence in which each nucleotide or amino acid is the one which occurs most frequently at that site in the different sequences which occur in nature. The phrase also refers to an actual sequence which approximates the theoretical consensus. A known CONSERVED SEQUENCE set is represented by a consensus sequence. Commonly observed supersecondary protein structures (AMINO ACID MOTIFS) are often formed by conserved sequences.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
Subunits of the antigenic determinant that are most easily recognized by the immune system and thus most influence the specificity of the induced antibody.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
A highly basic, 28 amino acid neuropeptide released from intestinal mucosa. It has a wide range of biological actions affecting the cardiovascular, gastrointestinal, and respiratory systems and is neuroprotective. It binds special receptors (RECEPTORS, VASOACTIVE INTESTINAL PEPTIDE).
A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Calcitonin gene-related peptide. A 37-amino acid peptide derived from the calcitonin gene. It occurs as a result of alternative processing of mRNA from the calcitonin gene. The neuropeptide is widely distributed in neural tissue of the brain, gut, perivascular nerves, and other tissue. The peptide produces multiple biological effects and has both circulatory and neurotransmitter modes of action. In particular, it is a potent endogenous vasodilator.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Peptides that have the ability to enter cells by crossing the plasma membrane directly, or through uptake by the endocytotic pathway.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
Development of a library collection, including the determination and coordination of selection policy, assessment of needs of users and potential users, collection use studies, collection evaluation, identification of collection needs, selection of materials, planning for resource sharing, collection maintenance and weeding, and budgeting.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
The study of the composition, chemical structures, and chemical reactions of living things.
The production of PEPTIDES or PROTEINS by the constituents of a living organism. The biosynthesis of proteins on RIBOSOMES following an RNA template is termed translation (TRANSLATION, GENETIC). There are other, non-ribosomal peptide biosynthesis (PEPTIDE BIOSYNTHESIS, NUCLEIC ACID-INDEPENDENT) mechanisms carried out by PEPTIDE SYNTHASES and PEPTIDYLTRANSFERASES. Further modifications of peptide chains yield functional peptide and protein molecules.
Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
A multistage process that includes the determination of a sequence (protein, carbohydrate, etc.), its fragmentation and analysis, and the interpretation of the resulting sequence information.
Transport proteins that carry specific substances in the blood or across cell membranes.
A 36-amino acid peptide produced by the L cells of the distal small intestine and colon. Peptide YY inhibits gastric and pancreatic secretion.
DNA analogs containing neutral amide backbone linkages composed of aminoethyl glycine units instead of the usual phosphodiester linkage of deoxyribose groups. Peptide nucleic acids have high biological stability and higher affinity for complementary DNA or RNA sequences than analogous DNA oligomers.
A cell line derived from cultured tumor cells.
The naturally occurring or experimentally induced replacement of one or more AMINO ACIDS in a protein with another. If a functionally equivalent amino acid is substituted, the protein may retain wild-type activity. Substitution may also diminish, enhance, or eliminate protein function. Experimentally induced substitution is often used to study enzyme activities and binding site properties.
Preclinical testing of drugs in experimental animals or in vitro for their biological and toxic effects and potential clinical applications.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
A PEPTIDE of 22 amino acids, derived mainly from cells of VASCULAR ENDOTHELIUM. It is also found in the BRAIN, major endocrine glands, and other tissues. It shares structural homology with ATRIAL NATRIURETIC FACTOR. It has vasorelaxant activity thus is important in the regulation of vascular tone and blood flow. Several high molecular weight forms containing the 22 amino acids have been identified.
Proteins that form the CAPSID of VIRUSES.
Proteins found in any species of bacterium.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Acquisition, organization, and preparation of library materials for use, including selection, weeding, cataloging, classification, and preservation.
Peptides that regulate the WATER-ELECTROLYTE BALANCE in the body, also known as natriuretic peptide hormones. Several have been sequenced (ATRIAL NATRIURETIC FACTOR; BRAIN NATRIURETIC PEPTIDE; C-TYPE NATRIURETIC PEPTIDE).
The process by which antigen is presented to lymphocytes in a form they can recognize. This is performed by antigen presenting cells (APCs). Some antigens require processing before they can be recognized. Antigen processing consists of ingestion and partial digestion of the antigen by the APC, followed by presentation of fragments on the cell surface. (From Rosen et al., Dictionary of Immunology, 1989)
Virulent bacteriophage and type species of the genus T7-like phages, in the family PODOVIRIDAE, that infects E. coli. It consists of linear double-stranded DNA, terminally redundant, and non-permuted.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
The use of automatic machines or processing devices in libraries. The automation may be applied to library administrative activities, office procedures, and delivery of library services to users.
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Regions of AMINO ACID SEQUENCE similarity in the SRC-FAMILY TYROSINE KINASES that fold into specific functional tertiary structures. The SH1 domain is a CATALYTIC DOMAIN. SH2 and SH3 domains are protein interaction domains. SH2 usually binds PHOSPHOTYROSINE-containing proteins and SH3 interacts with CYTOSKELETAL PROTEINS.
Incorporation of biotinyl groups into molecules.
Any member of the group of ENDOPEPTIDASES containing at the active site a serine residue involved in catalysis.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Neuropeptide and gut hormone that helps regulate GASTRIC ACID secretion and motor function. Once released from nerves in the antrum of the STOMACH, the neuropeptide stimulates release of GASTRIN from the GASTRIN-SECRETING CELLS.
Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES.
Ligases that catalyze the joining of adjacent AMINO ACIDS by the formation of carbon-nitrogen bonds between their carboxylic acid groups and amine groups.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
A family of G-protein-coupled receptors that was originally identified by its ability to bind N-formyl peptides such as N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE. Since N-formyl peptides are found in MITOCHONDRIA and BACTERIA, this class of receptors is believed to play a role in mediating cellular responses to cellular damage and bacterial invasion. However, non-formylated peptide ligands have also been found for this receptor class.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Print and non-print materials collected, processed, and stored by libraries. They comprise books, periodicals, pamphlets, reports, microforms, maps, manuscripts, motion pictures, and all other forms of audiovisual records. (Harrod, The Librarians' Glossary, 4th ed, p497)
A 27-amino acid peptide with histidine at the N-terminal and isoleucine amide at the C-terminal. The exact amino acid composition of the peptide is species dependent. The peptide is secreted in the intestine, but is found in the nervous system, many organs, and in the majority of peripheral tissues. It has a wide range of biological actions, affecting the cardiovascular, gastrointestinal, respiratory, and central nervous systems.
Systems for the delivery of drugs to target sites of pharmacological actions. Technologies employed include those concerning drug preparation, route of administration, site targeting, metabolism, and toxicity.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Extensive collections, reputedly complete, of facts and data garnered from material of a specialized subject area and made available for analysis and application. The collection can be automated by various contemporary methods for retrieval. The concept should be differentiated from DATABASES, BIBLIOGRAPHIC which is restricted to collections of bibliographic references.
Cell surface receptors that bind peptide messengers with high affinity and regulate intracellular signals which influence the behavior of cells.
Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.
Substances elaborated by viruses that have antigenic activity.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
A potent natriuretic and vasodilatory peptide or mixture of different-sized low molecular weight PEPTIDES derived from a common precursor and secreted mainly by the HEART ATRIUM. All these peptides share a sequence of about 20 AMINO ACIDS.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The processes triggered by interactions of ANTIBODIES with their ANTIGENS.
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The concentration of a compound needed to reduce population growth of organisms, including eukaryotic cells, by 50% in vitro. Though often expressed to denote in vitro antibacterial activity, it is also used as a benchmark for cytotoxicity to eukaryotic cells in culture.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.
Methods for determining interaction between PROTEINS.
A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.
A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.

Vascular endothelial growth factor (VEGF) receptor II-derived peptides inhibit VEGF. (1/2643)

Vascular endothelial growth factor (VEGF) directly stimulates endothelial cell proliferation and migration via tyrosine kinase receptors of the split kinase domain family. It mediates vascular growth and angiogenesis in the embryo but also in the adult in a variety of physiological and pathological conditions. The potential binding site of VEGF with its receptor was identified using cellulose-bound overlapping peptides of the extracytosolic part of the human vascular endothelial growth factor receptor II (VEGFR II). Thus, a peptide originating from the third globular domain of the VEGFR II comprising residues 247RTELNVGIDFNWEYP261 was revealed as contiguous sequence stretch, which bound 125I-VEGF165. A systematic replacement with L-amino acids within the peptide representing the putative VEGF-binding site on VEGFR II indicates Asp255 as the hydrophilic key residue for binding. The dimerized peptide (RTELNVGIDFNWEYPAS)2K inhibits VEGF165 binding with an IC50 of 0.5 microM on extracellular VEGFR II fragments and 30 microM on human umbilical vein cells. VEGF165-stimulated autophosphorylation of VEGFR II as well as proliferation and migration of microvascular endothelial cells was inhibited by the monomeric peptide RTELNVGIDFNWEYPASK at a half-maximal concentration of 3-10, 0.1, and 0.1 microM, respectively. We conclude that transduction of the VEGF165 signal can be interrupted with a peptide derived from the third Ig-like domain of VEGFR II by blockade of VEGF165 binding to its receptor.  (+info)

Cell-specific peptide binding by human neutrophils. (2/2643)

Analysis of peptide binding to human neutrophils (PMN) using phage display techniques has revealed cell-specific motifs reactive with the PMN surface. Phage libraries displaying either linear 9-mer or cyclic 10-mer and 6-mer peptides were incubated with normal human neutrophils followed by elution of bound phage with low pH (pH 2.2) and non-ionic detergent. Three rounds of selection generated several related peptide sequences that bound with high avidity to PMN. Using the linear 9-mer library, PMN-binding phage expressed peptides with the motif (G/A)PNLTGRW. The binding of phage bearing this motif was highly specific since no binding was observed on lymphocytes, fibroblasts, epithelial, or endothelial cells. Functional assays revealed that phage bearing the sequence FGPNLTGRW induced a pertussis toxin-sensitive increase in PMN cytosolic calcium analogous to that observed with Galphai coupled receptors. Other prominent motifs identified included phage bearing the consensus DLXTSK(M/L)X(V/I/L), where X represents a non-conserved position. Phage with this motif bound exclusively to a sub population of human PMN that comprised approximately 50% of the total and did not elicit a calcium response. The binding of such phage to PMN was prevented by co-incubation with competing peptides displaying identical or similar sequences (IC50 range from 0.6 micromol/L to 50 micromol/L for DLXTSK and GPNLTG, respectively). We speculate that these techniques will be useful in identifying functional cell-specific binding motifs and contribute to the development of new therapeutic and diagnostic strategies in human disease.  (+info)

Identification of a domain in guanylyl cyclase-activating protein 1 that interacts with a complex of guanylyl cyclase and tubulin in photoreceptors. (3/2643)

The membrane-bound guanylyl cyclase in rod photoreceptors is activated by guanylyl cyclase-activating protein 1 (GCAP-1) at low free [Ca2+]. GCAP-1 is a Ca2+-binding protein and belongs to the superfamily of EF-hand proteins. We created an oligopeptide library of overlapping peptides that encompass the entire amino acid sequence of GCAP-1. Peptides were used in competitive screening assays to identify interaction regions in GCAP-1 that directly bind the guanylyl cyclase in bovine photoreceptor cells. We found four regions in GCAP-1 that participate in regulating guanylyl cyclase. A 15-amino acid peptide located adjacent to the second EF-hand motif (Phe73-Lys87) was identified as the main interaction domain. Inhibition of GCAP-1-stimulated guanylyl cyclase activity by the peptide Phe73-Lys87 was completely relieved when an excess amount of GCAP-1 was added. An affinity column made from this peptide was able to bind a complex of photoreceptor guanylyl cyclase and tubulin. Using an anti-GCAP-1 antibody, we coimmunoprecipitated GCAP-1 with guanylyl cyclase and tubulin. Complex formation between GCAP-1 and guanylyl cyclase was observed independent of [Ca2+]. Our experiments suggest that there exists a tight association of guanylyl cyclase and tubulin in rod outer segments.  (+info)

Cell-free immunology: construction and in vitro expression of a PCR-based library encoding a single-chain antibody repertoire. (4/2643)

A novel cloning-independent strategy has been developed to generate a combinatorial library of PCR fragments encoding a murine single-chain antibody repertoire and express it directly in a cell-free system. The new approach provides an effective alternative to the techniques involving in vivo procedures of preparation and handling large libraries of antibodies. The possible use of the described strategy in the ribosome display is discussed.  (+info)

A fast, stochastic threading algorithm for proteins. (5/2643)

MOTIVATION: Sequences for new proteins are being determined at a rapid rate, as a result of the Human Genome Project, and related genome research. The ability to predict the three-dimensional structure of proteins from sequence alone would be useful in discovering and understanding their function. Threading, or fold recognition, aims to predict the tertiary structure of a protein by aligning its amino acid sequence with a large number of structures, and finding the best fit. This approach depends on obtaining good performance from both the scoring function, which simulates the free energy for given trial alignments, and the threading algorithm, which searches for the lowest-score alignment. It appears that current scoring functions and threading algorithms need improvement. RESULTS: This paper presents a new threading algorithm. Numerical tests demonstrate that it is more powerful than two popular approximate algorithms, and much faster than exact methods.  (+info)

Molecular cloning, cell localization and binding affinity to DNA replication proteins of the p36/LACK protective antigen from Leishmania infantum. (6/2643)

The p36/LACK antigen from Leishmania, an analogue of the receptor for activated protein kinase C (PKC), induces high levels of protection against parasite infection in the BALB/c mouse model. This protection is more than twice as high as that elicited by major parasite antigens such as soluble Leishmania antigen or the main surface protease gp63. We have cloned and purified p36/LACK from Leishmania infantum, the causative agent of visceral leishmaniasis in Europe. This protein belongs to the large family of WD 40 repeat proteins confined to eukaryotes and involved in numerous regulatory functions. Differential solubilization and immunofluorescence experiments indicate that p36/LACK is present close to the kinetoplast disc in the cell cytoplasm, probably bound to multiprotein complexes but not to membrane structures. These complexes probably also include cytoplasm PKC isoforms. The use of a genetically-encoded peptide library indicates that p36/LACK binds sequences present in several proteins involved in DNA replication and RNA synthesis. The recognition and binding sequences present in vacuolar proteins and at the beta-chain of major histocompatability complex (MHC) class II suggest the involvement of this regulatory protein in the early mechanisms triggering the protective immune response of the host against the parasite infection.  (+info)

Combinatorial protein engineering by incremental truncation. (7/2643)

We have developed a combinatorial approach, using incremental truncation libraries of overlapping N- and C-terminal gene fragments, that examines all possible bisection points within a given region of an enzyme that will allow the conversion of a monomeric enzyme into its functional heterodimer. This general method for enzyme bisection will have broad applications in the engineering of new catalytic functions through domain swapping and chemical synthesis of modified peptide fragments and in the study of enzyme evolution and protein folding. We have tested this methodology on Escherichia coli glycinamide ribonucleotide formyltransferase (PurN) and, by genetic selection, identified PurN heterodimers capable of glycinamide ribonucleotide transformylation. Two were chosen for physical characterization and were found to be comparable to the wild-type PurN monomer in terms of stability to denaturation, activity, and binding of substrate and cofactor. Sequence analysis of 18 randomly chosen, active PurN heterodimers revealed that the breakpoints primarily clustered in loops near the surface of the enzyme, that the breaks could result in the deletion of highly conserved residues and, most surprisingly, that the active site could be bisected.  (+info)

Divergence time estimates for the early history of animal phyla and the origin of plants, animals and fungi. (8/2643)

In the past, molecular clocks have been used to estimate divergence times among animal phyla, but those time estimates have varied widely (1200-670 million years ago, Ma). In order to obtain time estimates that are more robust, we have analysed a larger number of genes for divergences among three well-represented animal phyla, and among plants, animals and fungi. The time estimate for the chordate-arthropod divergence, using 50 genes, is 993 +/- 46 Ma. Nematodes were found to have diverged from the lineage leading to arthropods and chordates at 1177 +/- 79 Ma. Phylogenetic analyses also show that a basal position of nematodes has strong support (p > 99%) and is not the result of rate biases. The three-way split (relationships unresolved) of plants, animals and fungi was estimated at 1576 +/- 88 Ma. By inference, the basal animal phyla (Porifera, Cnidaria, Ctenophora) diverged between about 1200-1500 Ma. This suggests that at least six animal phyla originated deep in the Precambrian, more than 400 million years earlier than their first appearance in the fossil record.  (+info)

Epitope prediction based on random peptide library screening has become a focus as a promising method in immunoinformatics research. Some novel software and web-based servers have been proposed in recent years and have succeeded in given test cases. However, since the number of available mimotopes with the relevant structure of template-target complex is limited, a systematic evaluation of these methods is still absent. In this study, a new benchmark dataset was defined. Using this benchmark dataset and a representative dataset, five examples of the most popular epitope prediction software products which are based on random peptide library screening have been evaluated. Using the benchmark dataset, in no method did performance exceed a 0.42 precision and 0.37 sensitivity, and the MCC scores suggest that the epitope prediction results of these software programs are greater than random prediction about 0.09-0.13; while using the representative dataset, most of the values of these performance measures are
Based on years of experience in phage display technology, Creative Biolabs now is able to provide services for the construction and screening of immune antibody library.. With years of research and development in the past decades, phage display has been a powerful technology to display millions or even billions of different peptides or proteins. It is now a common choice for the studies of protein-protein, protein-peptide and protein-DNA interactions. Among all the applications of phage display technology, one of the most successful ones is the isolation of monoclonal antibodies utilizing large capacity phage antibody libraries.. Thanks to the rapid development of this technology, it has become a reliable tool for the production of monoclonal antibody with high specificity and affinity. Compared with conventional hybridoma technology, the generation of immune antibody libraries is not limited by the requirement of fusion partners. This expands the possibility to develop monoclonal antibodies ...
Having been researching in the field of phage display technology for years, Creative Biolabs is able to offer a series of comprehensive phage display library screening services by its own biopanning strategies and enhanced equipments.. Phage display technology is one of the major approaches applied in various protein interaction studies, especially in the discovery of specific antibodies, scaffolds and ligands. As binders with the desired specificity generally exist at low frequencies in the constructed libraries, phage display library screening has become an effective technique for the enrichment and identification of binders with high affinity and specificity from interested libraries.. Libraries from customized library construction services, in-house premade antibody libraries, peptide libraries or scaffold libraries, libraries provided by clients and other commercialized libraries can all be screened in Creative Biolabs.. No matter its purified or unpurified targets, we can screen for ...
This paper reports the synthesis and screening of a combinatorial peptide library for new affinity ligands for glycosylated haemoglobin (HbA(1c)), which is an important indicator of diabetes control. The new ligands are suitable for large-scale synthesis and overcome the disadvantages of antibodies (unstable and expensive to produce etc.), while remaining as efficient as antibodies in binding to the analyte. The library consisted of 262 144 hexapeptides synthesised using the one-bead-one-compound technique. The hexapeptides attached onto beads were screened with glycosylated haemoglobin HbA(1c). The structures of the peptides exhibiting high affinity were characterised by Edman microsequencing. Computer modelling simulation of one of the lead sequences has shown that this class of ligand has a high affinity and specificity for glycosylated haemoglobin. (C) 1998 Elsevier Science S.A. All rights reserved.. ...
Since the advent of phage display technology, dating back to 1985, antibody libraries displayed on filamentous phage surfaces have been used to identify specific binders for many different purposes, including the recognition of tumors. Phage display represents a high-throughput technique for screening billions of random fusion antibodies against virtually any target on the surface or inside cancer cells, or even soluble markers found in patient serum. Many phage display derived binders targeting important tumor markers have been identified. Selection directed to tumoral cells surfaces lead to the identification of unknown tumoral markers. Also the improvement of methods that require smaller amounts of cells has opened the possibility to use this approach on patient samples. Robust techniques combining an antibody library displayed on the phage surface and protein microarray allowed the identification of auto antibodies recognized by patient sera. Many Ab molecules directly or indirectly targeting
0054]Other methods, such as preparation of random peptide libraries or epitope libraries are well known in the art and may be used to reproducibly produce antigens. E.g., J. K. Scott & G. P. Smith, Searching for Peptide Ligands with an Epitope Library, 249 Science 386 (1990); J. J. Devlin et al., Random Peptide Libraries: A Source of Specific Protein Binding Molecules, 249 Science 404-406 (1990); S. E. Cwirla et al., Peptides on Phage: A Vast Library of Peptides for Identifying Ligands, 87 Proc. Natl Acad. Sci. USA 6378-6382 (1990); K. S. Lam et al., A New Type of Synthetic Peptide Library for Identifying Ligand-binding Activity, 354 Nature 82-84 (1991); S. Cabilly, Combinatorial Peptide Library Protocols (Humana Press, 304 pp, 1997); U.S. Pat. No. 5,885,780. Such libraries may be constructed by ligating synthetic oligonucleotides into an appropriate fusion phage. Fusion phages are filamentous bacteriophage vectors in which foreign sequences are cloned into phage gene III and displayed as part ...
TY - JOUR. T1 - Peptide mimotopes of oncoproteins as therapeutic agents in breast cancer.. AU - Ashok, B. T.. AU - David, L.. AU - Chen, Y. G.. AU - Garikapaty, V. P.. AU - Chander, B.. AU - Kanduc, D.. AU - Mittelman, A.. AU - Tiwari, R. K.. PY - 2003/4. Y1 - 2003/4. N2 - Generation of an immune response to oncoproteins can lead to a cancer specific protective immunity. Several such oncoproteins are being examined as tumor targets with mixed results. We are evaluating the clinical utility of synthetic peptides that would mimic the antigen immunologically and elicit a tumor specific immune response. HER-2/neu, an oncoprotein whose expression in breast cancer is associated with poor prognosis, lower disease free-survival and a propensity for metastases was chosen as a model. Antibodies, Ab2, Ab4 and Ab5 directed towards the extracellular domain of HER-2/neu were reacted to peptides from two synthetic phage display peptide libraries, LX-8 (12-mer peptide library containing disulfide bridge) and ...
TY - JOUR. T1 - Identification of peptide mimotopes of gp96 using single-chain antibody library. AU - Shanmugam, Arulkumaran. AU - Suriano, Robert. AU - Goswami, Neha. AU - Chaudhuri, Devyani. AU - Ashok, Badithe T.. AU - Rajoria, Shilpi. AU - George, Andrea L.. AU - Mittelman, Abraham. AU - Tiwari, Raj K.. N1 - Funding Information: Acknowledgments We gratefully acknowledge the financial assistance of AVT and the National Cancer Institute RO1 grant 1RO1CA131946 (RKT).. PY - 2011/3. Y1 - 2011/3. N2 - Heat shock proteins such as gp96 are immunogenic and are widely used as vaccines in immunotherapy of cancers. The present study focuses on the use of peptide mimotopes as immunotherapeutic vaccines for prostate cancer. To this end, we developed a 15-mer gp96 peptide mimotope specifically reactive to MAT-LyLu gp96-peptide complex using combinatorial single-chain antibody and peptide phage display library. The immunogenicity of the synthesized gp96 mimotope was analyzed initially in normal BALB/c mice ...
Rx Biosciences offers construction and screening of custom bacterial display libraries of small peptides. The library is useful in ligand discovery, antibody-antigen binding affinity study and identification of targets. Libraries of polypeptides displayed on the surface of bacteria are screened using flow cytometry or routinely used selection procedures (biopanning). The library is created by combining a highly diverse collection of synthetically-constructed randomized peptide sequences using a unique proprietary technique. The library has been specifically optimized to eliminate unwanted stop codons and aggregation-prone sequences. As in a phage display, the peptides the bacterial display peptides are expressed at the surface (plasma membrane) as a conjugated protein. The expression of the peptides is inducible.. We accept customer supplied vectors also and the customer owns the exclusive rights.. ...
628 Nasopharyngeal carcinoma (NPC) is one of the most common cancers among Chinese living in South China, Taiwan and Singapore. For treatment of NPC, although radiotherapy, surgical removal, and chemotherapy have been applied for decades and the 5 year survival rate in the advanced cases are still below 75 %. Much effort using other methods such as high dose chemotherapy plus bone marrow stem cell injection and other targeting therapy are needed to control this cancer. In the present experiment, we used random peptide phage display libraries to identify and isolate a specific novel peptide from NPC cell line for targeting drug delivery. After subtraction with control epithelial cells for 3 times, the remained phage libraries were biopanning on NPC-TW04 cells for 5 rounds. It was found that 44 selected phage clones could specifically bind to NPC cell surface. When ELISA was used to verify those clones, 16 phage clones showed higher affinity to NPC cells and were subjected to DNA sequencing. The ...
Two phage-displayed random peptide libraries were screened for ligands with potential inhibitory activity against pancreatic phospolipase A2 or ammodytoxin C (neurotoxin found in the venom glands of Vipera ammodytes ammodytes). The interaction of selected peptides with pancreatic phospholipase A2 and ammodytoxin C was confirmed with surface plasmon resonance and phage ELISA assays. Interestingly, peptides showed equal affinity to both proteins, regardless which of the two proteins was used as a target in the selection procedure. Despite pronounced affinity, none of the synthetic peptides inhibited enzyme targets in vitro at the concentrations below 167 [micro]M ...
TY - JOUR. T1 - Automated panning and screening procedure on microplates for antibody generation from phage display libraries. AU - Turunen, Laura. AU - Takkinen, Kristiina. AU - Söderlund, Hans. AU - Pulli, Timo. PY - 2009. Y1 - 2009. N2 - Antibody phage display technology is well established and widely used for selecting specific antibodies against desired targets. Using conventional manual methods, it is laborious to perform multiple selections with different antigens simultaneously. Furthermore, manual screening of the positive clones requires much effort. The authors describe optimized and automated procedures of these processes using a magnetic bead processor for the selection and a robotic station for the screening step. Both steps are performed in a 96-well microplate format. In addition, adopting the antibody phage display technology to automated platform polyethylene glycol precipitation of the enriched phage pool was unnecessary. For screening, an enzyme-linked immunosorbent assay ...
Our libraries developed from precision DNA fragments have enabled us to generate a peptide phage display system with unmatched complexity.
We plan to do some fishing into a random peptide library and would like to know the experience of those that have used constrained peptides vs unconstrained peptides. Are peptides that are removed from their constrained vectors likely to still function in an unconstrained context? ie is the greater likelyhood of getting a hit on the constrained library offset by the potential lack of function when the peptides are subsequently used unconstrained? -- Scott Vande Pol Case Western Reserve University Department of Pathology Cleveland Oh. 44106 sbv at pop.cwru.edu ...
A peptide library is a tool for protein-related study. A peptide library contains a great number of peptides that have a systematic combination of amino acids. Usually, peptide library is synthesized on solid phase, mostly on resin, which can be made as flat surface or beads. The peptide library provides a powerful tool for drug design, protein-protein interactions, and other biochemical as well as pharmaceutical applications. You can create a mix of desired amino acids at each point in a sequence. In this way, you could create a library of 20 different polypeptides with only one amino acid residue at random and the rest being the same. The usefulness of this form of peptide synthesis is limited as you cant go beyond 70 acids in length. This would give you 2070 possible combinations and thats only if you dont include the plethora of available amino acids with pre-installed post-translational modifications. Most drug development does not involve such a random assortment of proteins as you ...
A convenient method for synthesis of cyclic peptide libraries, protein peptide and polypeptide smallest to largest fraction, piruvato quinase glucagon like peptide, genscript custom peptide, nitrobenzyl cysteine peptide, hexahistidine peptide calculator, lupuzor peptide 1402, is erythropoietin a peptide hormone, antigenic peptide prediction bioinformatics tools blast, kinerase pro therapy c8 peptide intensive face treatment reviews, tat peptide sequence calculator, superdex peptide 10 300 gl column wraps, peptide antigen processing pathway, over the counter medications to treat anxiety, the proceedings of 20th american peptide symposium, antigenic peptide prediction server products, gap 27 peptide calculator, what stomach enzyme breaks down peptide bonds, igf 1 peptide therapy
The interaction between a CD4+ TH cell and an antigen presenting cell (APC) is a finely tuned event in adaptive immunity. The affinity is dictated by the T cell receptor (TCR) and the characteristics of antigenic peptide epitopes presented on the major histocompatibility complex class II (pMHC class II) molecules on APCs. Due to the high degree of polymorphism in MHC molecules and the vast repertoire of epitopes that may be presented on each, it is an immense challenge to predict epitopes relevant to disease and homeostasis. It would therefore be of very high value to develop a technology that allows for precise experimental identification of T cell epitopes in the absence of any prior knowledge of the relevant antigen, as long as one has access to T cells. We have developed a novel and generic combinatorial display system for MHC class II, based on phage display. Using phage display, one can probe an epitope sequence space orders of magnitude larger than any cellular or chemical system. As a ...
article{c02feb63-0c75-498d-9b63-6043d6df0ab2, abstract = {Background: Phage Display technology is a well established technique for high throughput screening of affinity ligands. Here we describe a new compact chromato-panning procedure for selection of suitable binders from a phage peptide display library. Results: Both phages and E. coli cells pass non-hindered through the interconnected pores of macroporous gel, so called cryogel. After coupling a ligand to a monolithic cryogel column, the phage library was applied on the column and non-bound phages were washed out. The selection of strong phage-binders was achieved already after the first panning cycle due to the efficient separation of phage-binders from phage-non-binders in chromatographic mode rather than in batch mode as in traditional biopanning procedures. E. coli cells were applied on the column for infection with the specifically bound phages. Conclusion: Chromato-panning allows combining several steps of the panning procedure ...
With the success of the human genome project, the focus of life science research has shifted to the functional and structural analyses of proteins, such as the fields of proteomics and structural genomics. These analyses of proteins, including newly identified proteins, are expected to contribute to the identification of therapeutically applicable proteins for various diseases. Thus, pharmacoproteomic-based drug discovery and the development of protein therapies has currently attracted a great deal of attention (1, 2, 3) . However, it is clinically difficult to use most bioactive proteins, such as tumor necrosis factor-α (TNF-α), as antitumor agents because of their very low stability and pleiotropic action in vivo (4 , 5) .. TNF-α was reported to exert a strong cytotoxicity to various kinds of tumor cells but not to normal cells in vitro and to cause hemorrhagic necrosis of certain transplanted solid tumors (6) . Thus, TNF-α has been considered a promising new drug for cancer therapy. On ...
The product of the HER2/Neu oncogene is a receptor tyrosine kinase that is amplified in 25-30% of human primary breast tumors. In this project? we have isolated the HER2/Neu kinase from Sf9 cells infected with a baculovirus expression vector. We probed the substrate specificity of the HER2/Neu kinase using two peptide libraries: (1) a soluble peptide library containing three degenerate positions N-terminal to tyrosine; and (2) a bead-supported combinatorial library possessing sis degenerate positions at P - 1, P - 2, P - 3, P + 1, P + 2, and P + 3. We identified four novel substrate sequences for HER2/Neu from the two peptide libraries. We synthesized these peptides as individual sequences and measured steady-state kinetic properties for phosphorylation by HER2/Neu. One of the peptides, AAEEIYAARRG, is the best synthetic peptide substrate reported to date for HER2/Neu. All of the sequences bear a resemblance to sites of autophosphorylation on HER2/Neu and related epidermal growth factor (EGF) ...
Directed evolution is a powerful method for engineering of specific affinity proteins such as antibodies and alternative scaffold proteins. For selections from combinatorial protein libraries, robust and high-throughput selection platforms are needed. An attractive technology for this purpose is cell surface display, offering many advantages, such as the quantitative isolation of high-affinity library members using flow-cytometric cell sorting. This thesis describes the development, evaluation and use of bacterial display technologies for the engineering of affinity proteins. Affinity proteins used in therapeutic and diagnostic applications commonly aim to specifically bind to disease-related drug targets. Angiogenesis, the formation of new blood vessels from pre-existing vasculature, is a critical process in various types of cancer and vascular eye disorders. Vascular Growth Factor Receptor 2 (VEGFR2) is one of the main regulators of angiogenesis. The first two studies presented in this thesis ...
Directed evolution is a powerful method for engineering of specific affinity proteins such as antibodies and alternative scaffold proteins. For selections from combinatorial protein libraries, robust and high-throughput selection platforms are needed. An attractive technology for this purpose is cell surface display, offering many advantages, such as the quantitative isolation of high-affinity library members using flow-cytometric cell sorting. This thesis describes the development, evaluation and use of bacterial display technologies for the engineering of affinity proteins.. Affinity proteins used in therapeutic and diagnostic applications commonly aim to specifically bind to disease-related drug targets. Angiogenesis, the formation of new blood vessels from pre-existing vasculature, is a critical process in various types of cancer and vascular eye disorders. Vascular Growth Factor Receptor 2 (VEGFR2) is one of the main regulators of angiogenesis. The first two studies presented in this thesis ...
Cell panning of phage-displayed antibody library is a powerful tool for the development of therapeutic and imaging agents since disease-related cell surface proteins in native complex conformation can be directly targeted. Here, we employed a strategy taking advantage of an integrated vector system which allows rapid conversion of scFv-displaying phage into scFv-Fc format for efficient cell-based scFv library selection on a tetraspanin protein, CD9. A mouse scFv library constructed by using a phagemid vector, pDR-D1 was subjected to cell panning against stable CD9 transfectant, and the scFv repertoire from the enriched phage pool was directly transferred to a mammalian cassette vector, pDR-OriP-Fc1. The resulting constructs enabled transient expression of enough amounts of scFv-Fcs in HEK293E cells, and flow cytometric screening of binders for CD9 transfectant could be performed simply by using the culture supernatants. All three clones selected from the screening showed correct CD9-specificity. They
LifeTein Peptide Library: SARS-CoV-2 Receptor Binding Domain, 4 Fragments [LT-V013] - Overlapping Peptide Library: The SARS-CoV-2 Receptor Binding Domain is divided in to 4 fragments of 20 amino acids per peptide. The resulting overlapping peptide libraries can then be used for processes including continuous and linear epitope mapping, antibody screening and characterization. Reference 1: A highly conserved cryptic epitope in the receptor-binding
Heparan sulfate (HS) binds and modulates the transport and activity of a large repertoire of regulatory proteins. The HS phage display antibodies are powerful tools for the analysis of native HS structure in situ; however, their epitopes are not well defined. Analysis of the binding specificities of a set of HS antibodies by competitive binding assays with well defined chemically modified heparins demonstrates that O-sulfates are essential for binding; however, increasing sulfation does not necessarily correlate with increased antibody reactivity. IC50 values for competition with double modified heparins were not predictable from IC50 values with corresponding singly modified heparins. Binding assays and immunohistochemistry revealed that individual antibodies recognize distinct epitopes and that these are not single linear sequences but families of structurally similar motifs in which subtle variations in sulfation and conformation modify the affinity of interaction. Modeling of the antibodies ...
Principal Investigator:ITOH Kunihiko, Project Period (FY):1996 - 1997, Research Category:Grant-in-Aid for Scientific Research (C), Section:一般, Research Field:応用薬理学・医療系薬学
Reconstitution of a neocytokine from the first 30 aa of IL-2 is very novel in the field of peptide agonists. Recently characterized peptides, with agonist activities related to cytokines of the hemopoietin family, have consisted up to this point of small mimetics of erythropoietin (EPO (28)) or thrombopoietin (29) selected by random phage display peptide libraries and a small nonpeptidyl mimic of G-CSF (30). These compounds act as full agonists in various biological assays, and their signaling pathways appear to be identical to those induced by the natural ligand, yet their amino acid sequences are not found in the primary sequence of the natural protein. The crystal structure of the 20-aa mimetic peptide of EPO and of the extracellular domain of the EPO receptor (EPOR) reveals a dimerization of the peptide that is bound to the homodimeric EPOR. In this study, it was shown that the peptide p1-30 contains a part of the native aa sequence of IL-2, forms a tetrameric structure that mimics a ...
Phage display is a method to discover peptide ligands while minimizing and optimizing the structure and function of proteins (Hallahan, 2003). The phage is used as a scaffold to display recombinant libraries of peptides and provides a means to recover and amplify the peptides that bind to putative receptor molecules in vivo. In vivo selection simultaneously provides positive and subtractive screens because organs and tissues such as tumors are spatially separated. Phage DNA can then be sequenced to determine the amino acid sequence of peptides on the capsid that have been recovered from tumors. The T7 phage display system exploits the T7 capsid protein as a scaffold to display peptides on the capsid protein unique to the 10B protein on the surface of the phage. Gene 10 encoding the capsid protein is cloned with a series of multiple cloning sites at the C-terminus of the 10B protein. The natural translational frame shift site within the capsid gene has been removed so that only a single form of ...
The aim of this work was to verify the possibility of applying affinity chromatography in bacteriophage purification, from the perspective of therapeutic purposes. Elution profiles of phages modified with specific affinity motifs (Figures 3,4,5 and 6) show substantially higher phage concentration in elution fractions compared to final washing samples. This indicates binding of modified phages to the affinity resins and effective elution with standard competitive agents. Thus, affinity tags can be successfully incorporated into the T4 phage capsid by the in vivo phage display technique and they strongly elevate bacteriophage affinity to a specific resin. Non-specific binding was also observed: unmodified phages or those modified with the non-specific tag were eluted with the titre 104-105 pfu/ml. Nevertheless, the unspecific binding is 102-105 times weaker than the specific one and importantly it does not interfere with the aim of preparation of purified anti-bacterial active bacteriophages for ...
We are leaders in the field of human antibody engineering and have been involved in many of the seminal discoveries in the field. We are experts in phage, yeast and lentivirus display platforms and panning procedures, human antibody library constructions, Fc engineering, humanization procedures and targeted siRNA delivery to name a few. We have constructed human antibody libraries with tens of billion members and have successfully isolated therapeutic human antibodies against over two dozen targets. We have also developed and utilize antibody expression systems in bacteria, mammalian, yeast and insect cells. Students and post-doctoral fellows join the Marasco lab to become skilled artisans in the field under Dr. Marascos mentorship. An overview of some of our antibody engineering tools can be found here. ...
Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...
Abstract. Currently, novel drug design focused on the searching pharmacological compounds acting via influence on GPCRs heteromers. The strategy allows obtaining highly selective effects since these heteromers appear only on specific cells and tissues. Therefore, human monoclonal scFv antibodies able to recognizing GPCRs heteromers may constitute a valuable tool in modern therapies. Antibody phage display technique together with high throughput screening play a key role in the development of clinically useful immunomolecules. Therefore in the present work we focused on the comparison of various strategies used for biopanning process during phage display procedure, dedicated to isolation scFv antibodies specifically recognizing GPCRs heteromers. Experiments were conducted in two different cell lines (CHO-K1 and HEK 293) and six various selection procedures were described. Elimination of nonspecific bindings constitutes a key point during the process. Results obtained duing selection conducted in ...
Adamalysin-like metalloproteinases with thrombospondin (TS) motifs (ADAMTS)-5 is the multi-domain metalloproteinase that most potently degrades aggrecan proteoglycan in the cartilage and its activity is implicated in the development of osteoarthritis (OA). To generate specific exosite inhibitors for it, we screened a phage display antibody library in the presence of the zinc-chelating active site-directed inhibitor GM6001 (Ilomastat) and isolated four highly selective inhibitory antibodies. Two antibodies were mapped to react with exosites in the catalytic/disintegrin domains (Cat/Dis) of the enzyme, one in the TS domain and one in the spacer domain (Sp). The antibody reacting with the Sp blocked the enzyme action only when aggrecan or the Escherichia coli-expressed aggrecan core protein were substrates, but not against a peptide substrate. The study with this antibody revealed the importance of the Sp for effective aggrecanolytic activity of ADAMTS-5 and that this domain does not interact with sulfated
In vivo screening of phage-displayed peptide libraries has revealed extensive molecular differences in the blood vessels of individual normal tissues. Pathological lesions also put their signature on the vasculature; in tumours, both blood and lymphatic vessels differ from normal vessels. The changes that characterize tumour blood vessels include selective expression of certain integrins. Peptides isolated by in vivo phage display for homing to tumours have been shown to be useful in directing therapeutic agents to experimental tumours. The targeting can enhance the efficacy of the therapy while reducing side effects. Phage screening has also revealed lung-specific vascular markers that promote tumour metastasis to the lungs by mediating specific adherence of tumour cells to the lung vasculature. These phage-screening studies have revealed a previously unsuspected degree of vascular specialization and provide potentially useful guidance devices for targeted therapies.. ...
The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface ...
The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface ...
In a previous study, a scFv phage display library against white spot syndrome virus (WSSV) was constructed and yielded a clone designated A I with conformational specificity against native but not denatured viral antigen. Although the clone A1 has been used successfully as a diagnostic antibody, its precise target antigen has not been elucidated. A different strategy was adopted involving the construction of a second T7 phage display library utilizing mRNA isolated from shrimp infected with WSSV. Following RT-PCR and T7 phage library construction, phages displaying the candidate epitope were selected with A I scFv. Since successive enrichment steps were not associated with an increased titer of the phages, enrichment after successive tests was confirmed by PCR resulting in the prefer-red selection of a specific DNA sequence encoding a novel nucleocapsid protein WSSV388. Immune electron microscopy revealed that WSSV388 is located on the nucleocapsid. This result demonstrated that unknown antigen ...
558117PRTArtificial Sequenceisolated from phage display libraries 1Ser Ser His Lys His Pro Val Thr Pro Arg Phe Phe Val Val Glu Ser 1 5 10 15Arg222PRTArtificial Sequenceisolated from phage display libraries 2Ser Ser Cys Asn Cys Tyr Val Thr Pro Asn Leu Leu Lys His Lys Cys 1 5 10 15Tyr Lys Ile Cys Ser Arg 20322PRTArtificial Sequenceisolated from phage display libraries 3Ser Ser Cys Ser His Asn His His Lys Leu Thr Ala Lys His Gln Val 1 5 10 15Ala His Lys Cys Ser Arg 20422PRTArtificial Sequenceisolated from phage display libraries 4Ser Ser Cys Asp Gln Asn Asp Ile Phe Tyr Thr Ser Lys Lys Ser His 1 5 10 15Lys Ser His Cys Ser Arg 20522PRTArtificial Sequenceisolated from phage display libraries 5Ser Ser Ser Ser Asp Val Tyr Leu Val Ser His Lys His His Leu Thr 1 5 10 15Arg His Asn Ser Ser Arg 20622PRTArtificial Sequenceisolated from phage display libraries 6Ser Ser Ser Asp Lys Cys His Lys His Trp Tyr Cys Tyr Glu Ser Lys 1 5 10 15Tyr Gly Gly Ser Ser Arg 20714PRTArtificial Sequenceisolated from phage display ...
We have recently identified peptide mimetics of the Cryptococcus neoformans capsular polysaccharide by screening phage display peptide libraries. 2H1, one of a large family of mAbs against the glucuronoxylomannan fraction (GXM), is highly protective and binds several peptide motifs. This study analyzes the immunologic properties of P601E (SYSWMYE), a peptide from the low affinity motif (W/YXWM/LYE) that has an extended cross-reactivity among anti-GXM mAbs and whose binding correlates with the protective potential of mAbs in experimental infection. P601E is a mimetic, since it competes for GXM binding to 2H1, but not a mimotope, since it does not elicit an anti-GXM response. Sequence analysis of 14 anti-P601E mAbs indicates that anti-P601E mAbs elicited in BALB/c mice have an order of homology with 2H1 of V kappa | J kappa || V(H) | J(H) | D. Further screening of a peptide library with anti-P601E mAbs isolated peptides having a motif almost identical to the peptide motif selected by 2H1. When these
The one-bead-one-compound (OBOC) combinatorial peptide library is a powerful tool to identify ligand and receptor interactions. Here, we applied the OBOC library technology to identify mimotopes specific to the immunoglobulin E (IgE) epitopes of the major shellfish allergen tropomyosin. OBOC peptide libraries with 8-12 amino acid residues were screened with serum samples from patients with shellfish allergy for IgE mimotopes of tropomyosin. Twenty-five mimotopes were identified from the screening and their binding reactivity to tropomyosin-specific IgE was confirmed by peptide ELISA. These mimotopes could be divided into seven clusters based on sequence homology, and epitope mapping by EpiSearch of the clustered mimotopes was performed to characterize and confirm the validity of mimotopes. Five out of six of the predicted epitopes were found to overlap with previously identified epitopes of tropomyosin. To further confirm the mimicry potential of mimotopes, BALB/c mice were immunized with ...
Methods Rat antiserum, raised against a 60-70 kDa band of partially purified GnSAF, was immobilised on anti-rat IgG Dynabeads. In addition, a human antibody, derived from a phage display antibody library and expressed by CHO-K1 cells, was immobilised on protein L agarose beads. Both antibodies were then used for 15 consecutive immunopurification steps with excess human granulosa-luteal cell-conditioned medium (GCM). In both cases 2 M NaI was used to elute the bound GCM proteins. The eluted fractions were bioassayed for GnSAF and inhibin bioactivities and the proteins separated by 1-D and 2-D gel electrophoresis. Spots and bands of interest were excised from the gels and identified by mass spectroscopic peptide mass mapping ...
Porcine parvovirus (PPV) is the major causative agent in a syndrome of reproductive failure in swine. Much has been learned about the structure and function of PPV in recent years, but nothing is known about the epitopes of the structural protein VP1, which is an important antigen of PPV. In this study, the monoclonal antibody C4 against VP1 of PPV was prepared and was used to biopan a 12-mer phage peptide library three times. The selected phage clones were identified by ELISA and then sequencing. The amino acid sequences detected by phage display were analyzed, and a mimic immuno-dominant epitope was identified. The epitope of VP1 is located in the N-terminal and contains the role amino acid sequence R-K-R. Immunization of mice indicated that the phage-displayed peptide induces antibodies against PPV. This study shows that peptide mimotopes have potential as alternatives to the complex antigens currently used for diagnosis of PPV infection or for development of vaccines.
Dr. Marilena Hall received her B.S. in Chemistry in 1992 from McGill University in Montreal, Canada. She then earned her Ph.D. at the California Institute of Technology (NSERC Fellowship) where she worked in the laboratory of Dr. Jacqueline K. Barton. Her thesis involved the design of a synthetic deoxyribonuclease using a chimera of a DNA-binding rhodium complex and a short Zn2+ -coordinating peptide.. From 1998-2000, Dr. Hall carried out post-doctoral research at New England Biolabs in Beverly, MA creating an artificial bifunctional intein capable of both protein splicing and homing endonuclease activity. During her post-doc, she was also an adjunct professor at Massasoit Community College, teaching general chemistry from 1999-2000. Professor Hall joined the Stonehill Department of Chemistry in the Fall of 2000.. Professor Halls research program employs peptide phage display libraries to identify short peptides that can mimic the coordination of Zn2+ in zinc-containing metalloenzymes.. Several ...
The blood-brain barrier (BBB) is a biological barrier that protects the brain from neurotoxic agents and regulates the influx and efflux of molecules required for its correct function. This stringent regulation hampers the passage of brain parenchyma-targeting drugs across the BBB. BBB shuttles have been proposed as a way to overcome this hurdle because these peptides can not only cross the BBB but also carry molecules which would otherwise be unable to cross the barrier unaided. Here we developed a new high-throughput screening methodology to identify new peptide BBB shuttles in a broadly unexplored chemical space. By introducing d-amino acids, this approach screens only protease-resistant peptides. This methodology combines combinatorial chemistry for peptide library synthesis, in vitro models mimicking the BBB for library evaluation and state-of-the-art mass spectrometry techniques to identify those peptides able to cross the in vitro assays. BBB shuttle synthesis was performed by the ...
Mimotopes online peptide superstore provides custom peptide synthesis, peptide library synthesis and peptide reagents for immunology, drug discovery, bioassay development, epitope mapping, proteomics, target discovery and lead optimization.
Peptides , SARS-CoV2,COVID-19 Peptides , 96-well Overlapping Peptide Library; Specifications: Spike glycoprotein from SARS-CoV-2: NCBI the RefSeq (YP_009724390.1) of 1273 aa Peptide length: 15 aa, 9aa for the C-terminal peptide. Gross peptide weight: not quantified Synthesis scale: 5 mol Offset number: 7 aa Format: lyophilized in 96-well plate (P-DW-20-C Axygen ) Counter ion: TFA QC validation: MALDI-TOF QC on 10 % of peptidesCoronavirus Spike glycoproteins mediate the virus attachment to host cell surface receptors and facilitate the virus entry by assisting fusion between viral and host cell membranes. They are composed of two subunits, S1 and S2. The Spike protein of SARS-CoV-2 harbors furin cleavage sites, one being located at the boundary between S1 and S2.
Press Release issued Oct 16, 2017: Persistence Market Research (PMR), in its latest report, projects that the global antibody library technologies market revenue will increase at a CAGR of 4.4% between 2016 and 2024. Some of the latest antibody library design and selection methods enable easy identification of any MAB with any specificity, which is why pharmaceutical companies are increasingly making use of such technologies in their facilities.
Specifying 14-3-3: A fragment-based combinatorial peptide microarray generates affinity-based fingerprints of seven mammalian 14-3-3 isoforms. High-affinity motifs are identified against the highly homologous isoforms. Putative 14-3-3σ-specific peptides were also delineated by a dual-color ratiometric screening strategy (see picture). (Figure Presented) © 2008 Wiley-VCH Verlag GmbH & Co. KGaA ...
Monoclonal antibodies developed for therapeutic or diagnostic purposes need to demonstrate highly defined binding specificity profiles. Engineering of an antibody to enhance or reduce binding to related antigens is often needed to achieve the desired biologic activity without safety concern. Here, we describe a deep sequencing-aided engineering strategy to fine-tune the specificity of an angiopoietin-2 (Ang2)/vascular endothelial growth factor (VEGF) dual action Fab, 5A12.1 for the treatment of age-related macular degeneration. This antibody utilizes overlapping complementarity-determining region (CDR) sites for dual Ang2/VEGF interaction with KD in the sub-nanomolar range. However, it also exhibits significant (KD of 4 nM) binding to angiopoietin-1, which has high sequence identity with Ang2. We generated a large phage-displayed library of 5A12.1 Fab variants with all possible single mutations in the 6 CDRs. By tracking the change of prevalence of each mutation during various selection ...
TY - JOUR. T1 - Heat-shock proteins as dendritic cell-targeting vaccines - getting warmer. AU - McNulty, Shaun. AU - Colaco, Camilo. AU - Blandford, Lucy. AU - Bailey, Christopher. AU - Baschieri, Selene. AU - Todryk, Stephen. PY - 2013. Y1 - 2013. N2 - Heat-shock proteins (hsp) provide a natural link between innate and adaptive immune responses by combining the ideal properties of antigen carriage (chaperoning), targeting and activation of antigen-presenting cells (APC), including dendritic cells (DC). Targeting is achieved through binding of hsp to distinct cell surface receptors and is followed by antigen internalization, processing and presentation. An improved understanding of the interaction of hsp with DC has driven the development of numerous hsp-containing vaccines, designed to deliver antigens directly to DC. Studies in mice have shown that for cancers, such vaccines generate impressive immune responses and protection from tumour challenge. However, translation to human use, as for ...
Along with the immunizations been completed by ICA, Creative Biolabs was contracted by Virbac to discovery specific monoclonal antibodies through its Magic™ Antibody Discovery Platform. To meet the specific requirement of this project, Creative Biolabs adopted its advanced advanced phage display technology using extracted RNA sent from ICA to construct an immune antibody library for specific antibody isolation. This one-stop service covers phage display library construction, biopanning, single clone validation, DNA sequencing and quality control to discover specific antibodies against the interested antigen in the monoclonal format. A simple sketch of this process is shown below.. About Creative Biolabs. Creative Biolabs is a professional service provider in developing highly specific, high-affinity monoclonal antibodies from all popular antibody production species, including rabbit, chicken, llama, camel, alpaca, cow, dog, mouse, rat, sheep, monkey, human, and even shark. Creative Biolabs ...
Factor Xa subsite mapping by proteome-derived peptide libraries improved using WebPICS, a resource for proteomic identification of cleavage ...
Dr. Scott received her PhD for work on the germline immunoglobulin V genes. She attended medical school with the goal of becoming an academic biomedical researcher. Her postdoctoral research included projects to analyze the spectra of mutational hot-spots (W.G. Thilly), the development of the first phage-displayed peptide libraries and their use in analyzing antibody specificity (G.P. Smith) and in developing peptide mimics of a discontinuous protein epitope (E.D. Getzoff & J.A. Tainer). She began working at SFU in 1993 as an Assistant Professor in the Dept. of Chemistry and Member of the Institute of Molecular Biology & Biochemistry at SFU. (The Institute became a Department in SFUs Faculty of Science in 2001.) Dr. Scott was promoted to Associate Professor in 1998 and to Professor in 2002. In 2004, Dr. Scott began a joint appointment in the newly-formed Faculty of Health Sciences, as one of its founding faculty members. That year, she was awarded a Canada Research Chair in Molecular ...
CD28 is one of the key molecules for co-stimulatory signalling in T cells. Here, novel ligands (affibodies) showing selective binding to human CD28 (hCD28) have been selected by phage display technology from a protein library constructed through combinatorial mutagenesis of a 58-residue three-helix bundle domain derived from staphylococcal protein A. Analysis of selected affibodies showed a marked sequence homology and biosensor analyses showed that all investigated affibodies bound to hCD28 with micromolar affinities (K-D). No cross-reactivity towards the related protein human CTLA-4 could be observed. This lack of cross-reactivity to hCTLA-4 suggests that the recognition site on hCD28 for the affibodies resides outside the conserved MYPPPYY motif. The apparent binding affinity for hCD28 could be improved through fusion to an Fc fragment fusion partner, resulting in a divalent presentation of the affibody ligand. For the majority of selected anti-CD28 affibodies, in co-culture experiments ...
Rx Biosciences provides quality custom phage display library construction and screening services for biological research and drug discovery related projects. The gene of interest is randomly mutated to produce various combinations of the peptides or small antibodies [e.g. scFv and Fab] or proteins which get displayed on the surface of a filamentous phage [M13, fd, and f1 strains] as fusion proteins. Using a binding affinity-based process called panning; a small number of phages that display proteins specifically binding to a target of interest are recovered from the phage library that usually has a repertoire of many billions of unique displayed proteins. Finally, the proteins displayed by the selected phages are identified by phage amplification followed by DNA sequencing.. ...
Combinatorial protein engineering, taking advantage of large libraries of protein variants and powerful selection technology, is a useful strategy for developing affinity proteins for applications in biotechnology and medicine. In this thesis, two small affinity proteins have been subjected to combinatorial protein engineering to improve or redirect the binding. In two of the projects, a three-helix protein domain based on staphylococcal protein A has been used as scaffold to generate so called Affibody molecules capable of binding to key proteins related to two diseases common among elderly people.. In the first project, Affibody molecules were selected using phage display technology for binding to Ab-peptides, believed to play a crucial role in Alzheimers disease, in that they can oligomerize and contribute to the formation of neural plaques in the brain. The selected Affibody molecules were found to efficiently capture Ab from spiked human plasma when coupled to an affinity resin. The ...
Video articles in JoVE about sequence homology include Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii, Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group, Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing, Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS, Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast, Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins, Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA, Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates, Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity, Purifying the Impure:
mAbs. Col-1, an immunoglobulin G (IgG)-2a mAb, was purchased from Zymed. The isotype control IgG2a was obtained from Sigma and used as a control antibody.. Phage library and biopanning. Three successive rounds of biopanning with the anti-CEA antibody Col-1 were done with two pIII-display peptide phage libraries: CL10 and LL9. CL10 expresses cysteine-flanked decapeptides circularized by disulfide bridges and LL9 comprises nine linear peptides. The libraries were applied separately or pooled for biopannings. They were kindly provided by Prof. Dr. Luca Mazzucchelli (University of Bern, Bern, Switzerland; ref. 18). Biopannings were done as outlined in ref. 19, with slight modifications. In short, ELISA plates (Nunc) were coated with 40 μg/mL Col-1 in bicarbonate buffer (pH 8.5). Wells were blocked with PBS/1% dry milk and incubated with an aliquot of the phage library (∼5 × 1010 phage particles) in PBS/1% dry milk/0.1% Tween 20 at 37°C and 4°C for 1 h each. Unbound phage particles were removed ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
The subject invention provides novel and advantageous methods for identifying amino acid sequences in random peptide libraries that can bind to Gag polypeptides. The subject invention also establishes a novel in vitro system that can be used to test competitive inhibitors of retrovrial capsid assembly. Also provided are peptides, and compositions containing these peptides, which are inhibitors of the retrovirus Gag protein(s) function. Chimeric Gag polypeptides are also provided.
The designing, synthesizing and screening of one-bead-one-compound (OBOC) combinatorial chemistry libraries against a single target ligand has been well developed. Recently, novel cholesterol/peptide hybrid combinatorial One-Bead-One-Compound (OBOC) combinatorial libraries have been developed and synthesized with self folding capabilities. The library design strategy was based on a similar pentamer and hexamer self-folding branched tricylic libraries previously developed. The cholic acid on the side chain of the carboxyl lysine is believed to interact with fixed hydrophobic amino acids at the amino-termini (position 5) of the twin branched L-amino acid arms and self-fold into a tricyclic molecule. The newly synthesized library has arginine (R) and Lysine (K) down-proportioned to 10 % for each position to decrease the probability of positive charge nonspecific binding. Thirty L-, D-, and unnatural amino acids were used in each position as building blocks. Hydrophobicity was fixed at position 5 ...
In this study of diverse antibody libraries, the authors demonstrate that by targeting different residues of the same antibody gene, it is possible to create sublibraries with distinct profiles in terms of the number and affinities of the library clones against different-sized antigens. Apparent affinities for anti-streptavidin clones were measured on the BLItz system using Streptavidin biosensors.
TY - JOUR. T1 - Identification of anti-CA125 antibody responses in ovarian cancer patients by a novel deep sequence-coupled biopanning platform. AU - Frietze, Kathryn M.. AU - Roden, Richard B.S.. AU - Lee, Ji Hyun. AU - Shi, Yang. AU - Peabody, David S.. AU - Chackerian, Bryce. N1 - Funding Information: K.M. Frietze was supported by a University of New Mexico Cancer Center Postdoctoral Matching Grant. This study was funded, in part, by NIH grant R01AI083305 to B. Chackerian. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.. PY - 2016/2. Y1 - 2016/2. N2 - High-grade epithelial ovarian cancer kills more women than any other gynecologic cancer and is rarely diagnosed at an early stage. We sought to identify tumor-associated antigens (TAA) as candidate diagnostic and/or immunotherapeutic targets by taking advantage of tumor ...
Evolutionary processes, and specifically selection-based mechanisms, have long served to inspire in vitro methods for generating proteins and nucleic acids that mediate functions of interest. Examples going back two decades include the development of phage display methods (Scott and Smith, 1990) and methods based on selection of RNA molecules (Ellington and Szostak, 1990; Tuerk and Gold, 1990) from libraries of enormous structural diversity (as many as1010 distinct molecular structures). A recent review (Dreier and Plückthun, 2011) provides a sense of what can be accomplished with one these methods, ribosome display, in terms of generating macromolecules, such as antibody fragments, with desired functional properties (e.g., high-affinity binding to a target molecule).. However, these methods typically involve repeated and somewhat tedious interventions by the investigators to progress from one round of molecular variant generation and selection to the next. The overall process can be quite ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Plant Cell Environ., 38: 375-384, 2015. Full text Arabidopsis thaliana root cell wall proteomics: increasing the proteome coverage using a combinatorial peptide ligand library and description of unexpected Hyp in peroxidase amino acid sequences. ...
Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...
Abstract: The design of compounds that form cytotoxic, non-mutagenic 3-methyladenine adducts in pancreatic ß-cells is being studied in this project for potential applications in the treatment of diseases such as diabetes and cancer. These compounds are composed of three components: 1) a cell-targeting moiety, glucosamine, which targets the insulin producing pancreatic ß-cells by way of the GLUT-2 transporters present on these cells 2) a site-specific DNA methylating agent, Me-Lex, which has been shown to selectively produce cytotoxic, non-mutagenic N3- methyladenine adducts 3) a linker component that connects the two other components together. The linker is a critical component because it has to be such that the cell-targeting and DNAmethylating properties of the two functional components are maintained. A synthetic route was explored, which enables the easy introduction of various linkers into the molecules. Fluorescent compounds were also designed to bind weakly to DNA at the same positions ...
Thank you for sharing this Cancer Research article.. NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.. ...
Although peptides are well recognised biological molecules in vivo, their selection from libraries is challenging because of relative low affinity whilst in linear conformation. We hypothesized that multiplexed peptides and DNA on the surface of beads would provide a platform for enhanced avidity and the selection of relevant peptides from a library (ORBIT bead display). Using human immunodeficiency virus (HIV-1) gp120 as a target, we identify peptides that inhibit HIV-1 replication in vitro through blocking of protein:protein interaction with the co-receptor CCR5. The bead display approach has many potential applications for probing biological systems and for drug lead development.
Antibody building blocks stuck together with bacterial superglue can protect against bunyaviruses, and herald a new approach to developing antibodies to fight emerging viruses.
The Lippincott Interactive Anesthesia Library on CD-ROM Version 3.0 : The Lippincott Interactive Anesthesia Library on CD-ROM Version 3.0 Pub Da
Reaxense Inc - a one-stop for comprehensive product range in medicinal chemistry, small molecule screening and drug discovery. We offer personalised custom synthesis, fragment libraries, focused and targeted libraries, custom synthesis and chemical sourcing.
TY - JOUR. T1 - Rapid simultaneous screening for DNA integrity and antigen specificity of clones selected by phage display. AU - Fischer, P.. AU - Leu, S. J.C.. AU - Yang, Y. Y.. AU - Chen, P. P.. N1 - Copyright: Copyright 2004 Elsevier B.V., All rights reserved.. PY - 1994. Y1 - 1994. UR - http://www.scopus.com/inward/record.url?scp=0028180282&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0028180282&partnerID=8YFLogxK. M3 - Article. C2 - 7520712. AN - SCOPUS:0028180282. VL - 16. SP - 828+830. JO - BioTechniques. JF - BioTechniques. SN - 0736-6205. IS - 5. ER - ...
Do you need peptides synthesized? Peptide antigens for antibody production? Or even specialized peptide libraries? -- The UNC High Throughput Peptide Synthesis and Array Facility can help!
|p||strong|Introduction|/strong||br /|Recombinant antibody technologies have been widely used to produce various single-chain Fv or Fab antibody fragments of different specificity. The randomized combination of cloned variable heavy and light chain imm
Phage display is a convenient method to select proteins of interest based on binding affinity. Through subsequent rounds of panning and ELISA assays, protein library members with the desired binding activity are retained on the immobilized target, while the non-binding members are washed off. The bacteriophage serves as a physical link between the gene of interest and the protein product, since the latter is expressed on the coat of the phage thanks to its fusion with the gIII residue. We have recently used this technology to discover an antibody fragment (scFv), called H5, capable of selectively binding to the integrin binding domain of fibronectin (FN) under a strained configuration, typically due to forces exerted on the extracellular matrix (ECM) by cells.. FN is a highly elastic protein that forms fibers and contributes to the ECM. The elasticity of the protein is derived from the so-called type III repeats within the protein. The 9th and 10th type III repeats possess the PHSRN synergy ...
Learn information about Fab antibody fragment, covering definition, molecular weight, structure, expression, production, purification and services.
The key to preventing epidemic is the ability to diagnose the infected early to preempt further propagation. For this, Bio-Synthesis, Inc. provides primers and probes (as well as synthetic RNA control) for COVID-19 diagnosis via RT-PCR assay. For medicinal chemistry, it specializes in peptide synthesis, characterization, modification, purification to generate various peptide-based building blocks as well as pharmaceutical intermediates-in addition to peptide libraries, peptide arrays, peptidomimetics. Antibody purification, characterization/quantification, modification and labeling are also offered. It specializes in oligonucleotide modification and provides an extensive array of chemically modified nucleoside analogues (over ~200) including bridged nucleic acid (BNA). A number of options are available to label oligonucleotides (DNA or RNA) with fluorophores either terminally or internally as well as conjugate to peptides. It recently acquired a license from BNA Inc. of Osaka, Japan, for the ...
ProbesOnline for April 2010. In this issue you will find new product information for Applied Biosystems® Attune™ Acoustic Focusing Cytometer, Click-iT® Nascent RNA Capture Kit, LC3B Antibody Kit for Autophagy, BacMam 2.0 GFP Transduction Control
Protection against disease-causing pathogens, known as immunity, involves numerous cells organs, tissues and their products. To able to understand the biology of immune cells (hematopoietic cells) and their role in an immune system, we have used several different methods, including transcriptome analyses, bioinformatics, production of recombinant proteins and analyses of some of them, focusing on the granule proteases by substrate phage display.. Hematopoietic cells express surface receptors interacting with the constant region of immunoglobulins (Igs) known as Fc receptors (FcRs). These receptors play major roles in the immune system, including enhancing phagocytosis, activating antibody dependent cellular cytotoxicity and cell activation. A detailed bioinformatics analysis of FcRs reveals that the poly-Ig receptors (PIGR), FcR-like molecules and common signalling γ chain all appeared very early with the appearance of the bony fishes, and thereby represent the first major evolutionary step in ...
Use of Oriented Peptide Libraries to determine phosphopeptide binding specificity and protein kinase substrate specificity[edit ... In later research, the oriented peptide library approach has also been used to characterize protease cleavage specificity.[32] ... 2.2 Use of Oriented Peptide Libraries to determine phosphopeptide binding specificity and protein kinase substrate specificity ... peptide library approach was extended to identify the substrate specificity of protein kinases toward synthetic peptides.[28] ...
The Journal of Peptide Research. Wiley Online Library. 58 (3): 193-203. doi:10.1034/j.1399-3011.2001.00912.x. Payandeh J, ... The peptide was built based on the sequence of a 20 amino acid residue from the Drosophila melanogaster 's Shaker ShB protein ... The peptide restored inactivation to the channel, giving further support to the ball and chain model. In β2 proteins, the first ... Wiley Online Library. 59 (5): 380-393. doi:10.1002/1097-0282(20011015)59:5. 3.0.CO;2-T. Miyamoto K, Nakagawa T, Kuroda Y (2001 ...
... isotopically-labelled peptides and peptide libraries. Sigma Advanced Genetic Engineering (SAGE) Labs is a division within Sigma ... ISOTEC provides isotopically labeled products for protein structure determination, peptide synthesis, proteomics, metabolic ... peptides, amino acids and their derivatives. Sigma RBI produces specialized products for use in the field of cell signaling and ...
... fruit with combinatorial peptide ligand libraries". Electrophoresis. 34 (2): 207-14. doi:10.1002/elps.201200389. PMID 23161558 ...
2001). "Characterization of glucokinase-binding protein epitopes by a phage-displayed peptide library. Identification of 6- ... "National Center for Biotechnology Information, U.S. National Library of Medicine.. *^ a b "Entrez Gene: PFKFB2 6-phosphofructo- ... "National Center for Biotechnology Information, U.S. National Library of Medicine.. .mw-parser-output cite.citation{font-style: ...
ISBN 3-527-29869-X. G. Jung (ed.): Combinatorial Peptide and Nonpeptide Libraries, A Handbook. Wiley-VCH, Weinheim. 1996. ISBN ... The sequence motifs of MHC-bound natural peptide libraries (major histocompatibility complex) has been determined for the first ... peptide libraries, complete B- and T-cell epitope mapping of viral proteins, immunobiochemistry. ... pool sequencing of synthetic and natural peptide libraries". Anal Biochem. 212 (1): 212-220. doi:10.1006/abio.1993.1314. PMID ...
"T7 displayed peptides as targets for selecting peptide specific scFvs from M13 scFv display libraries". J. Immunol. Methods. ... Peptides are usually fused to the N-terminus of pVIII. Usually peptides that can be fused to pVIII are 6-8 amino acids long. ... A patent by George Pieczenik claiming priority from 1985 also describes the generation of peptide libraries. Like the two- ... In 1990, Jamie Scott and George Smith described creation of large random peptide libraries displayed on filamentous phage. ...
These are added in sequence onto the growing peptide chain, which is attached to a solid resin support. Libraries of peptides ... "Past and future perspectives of synthetic peptide libraries". Current Protein & Peptide Science. 9 (5): 447-467. doi:10.2174/ ... However, not all peptide bonds are formed in this way. In a few cases, peptides are synthesized by specific enzymes. For ... In chemistry, peptides are synthesized by a variety of reactions. One of the most-used in solid-phase peptide synthesis uses ...
Escoubas P (November 2006). "Molecular diversification in spider venoms: a web of combinatorial peptide libraries". Molecular ...
"Phage-encoded combinatorial chemical libraries based on bicyclic peptides". Nature Chemical Biology. 5 (7): 502-507. doi: ... subscription or UK public library membership required) (subscription required) "Sir Gregory P. Winter - Facts - 2018". ...
All peptide sequences obtained from biopanning using combinatorial peptide libraries have been stored in a special freely ... Libraries of peptides and proteins displayed on filamentous phage. Methods in Enzymology. 1993. 217:228-257. ... Biopanning involves 4 major steps for peptide selection. The first step is to have phage display libraries prepared. This ... Mandecki W, Chen YC, and Grihalde N. A Mathematical Model for Biopanning (Affinity Selection) Using Peptide Libraries on ...
A DNA library for any protein or small peptide of interest can be synthesized by solid-phase synthesis followed by PCR ... They allow a library size as large as 10^15 different members. The large library size increases the probability to select very ... For example, the library size for phage and bacterial display is limited to 1-10 × 10^9 different members. The library size for ... The T7 promoter region allows large-scale in vitro T7 transcription to transcribe the DNA library into an mRNA library, which ...
"A Pilot Screen of a Novel Peptide Hormone Library Identified Candidate GPR83 Ligands". SLAS DISCOVERY: Advancing the Science of ...
"A cell-penetrating peptide from a novel pVII-pIX phage-displayed random peptide library". Bioorganic & Medicinal Chemistry. 10 ... By panning this library against a B lymphoctye cell line, a unique cell-binding and internalizing peptide was discovered. ... These findings were crucial to his group's publication on the glycation of the amyloid β-peptide by nornicotine, proposing the ... Janda has also worked creating peptide and antibody molecules for the treatment of cancer. By employing a novel approach, he ...
"PSMA mimotope isolated from phage displayed peptide library can induce PSMA specific immune response". Cell Research. 9 (4): ... synthetic bioactive peptides, and Enzyme linked immunosorbent assay (ELISA) kits for research as well as diagnostic and ...
The problem of generating and sequencing large libraries of peptides suitable for pharmaceutical work remained. Selection and ... peptides capturable to contemporary protein microarrays through the creation of synthetic peptide combinatorial libraries (SPCL ... those formed by sources found in the wild and brought into the library), creation of the requisite number of peptides for new ... The peptide is thus elongated one amino acid at each step, and by careful movement of each teabag, a series of related peptides ...
"An in vitro polysome display system for identifying ligands from very large peptide libraries". Proceedings of the National ... However, the DNA library coding for a particular library of binding proteins is genetically fused to a spacer sequence lacking ... peptides (Mattheakis, Bhatt and Dow) As it is performed entirely in vitro, there are two main advantages over other selection ... First, the diversity of the library is not limited by the transformation efficiency of bacterial cells, but only by the number ...
By screening a combinatorial ShK peptide library, novel analogues were identified, which when fused to the C-termini of IgG1-Fc ... ShK-related peptides in parasitic worms: AcK1, a 51-residue peptide from hookworms Ancylostoma caninum and Ancylostoma ... Some K+ channel-blocking peptides lack the functional dyad, but even in these peptides a lysine physically blocks the channel, ... "Autocrine-Based Selection of Drugs That Target Ion Channels from Combinatorial Venom Peptide Libraries". Angewandte Chemie. 55 ...
"Substrate specificity of the integral membrane protease OmpT determined by spatially addressed peptide libraries". Biochemistry ... Once docked in this position, water is positioned to attack the peptide in the active site. The cleavage of peptide bonds by ... The peptide bond cleavage occurs via the nucleophilic attack of water at the carbonyl between two adjacent amino acid residues ... Because of the specificity of the active site, OmpT does not act on peptides with a negatively charged residue adjacent to the ...
This approach was also used in screening peptide libraries. The tethered peptide library was tested with a dissolved target ... In omission libraries a certain amino acid is missing from all peptides of the mixture. The figure shows the full library and ... If we deal with a non-peptide organic libraries library it is not as simple to determine the identity of the content of a bead ... Cwirla, S. E.; Peters, E. A.; Barrett, R. W.; Dower, W. J. (1990-08-01). "Peptides on phage: a vast library of peptides for ...
Rapid selection and identification of functional CD8+ T cell epitopes from large peptide-coding libraries. Nat Comm, 2019. 10: ...
Peptide+Elongation+Factor+G at the US National Library of Medicine Medical Subject Headings (MeSH). ... The nascent peptide continues to fold and leaves the 70S ribosome, the mRNA, the deacylated tRNA (P site), and the Class I ... A Class I release factor (RF1 or RF2) binds to the stop codon, which induces hydrolysis of the tRNA-peptide bond in the P site ... In this process, the peptidyl transferase center (PTC) has catalyzed the formation of a peptide bond between amino acids, ...
2005). "Comparison of Bacterial and Phage Display Peptide Libraries in Search of Target-Binding Motif". Applied Biochemistry ... fused peptides with linkers with the ompA gene, causing the peptides to be expressed in the OmpA proteins. They showed that the ... Peptides are very useful as therapeutic and diagnostic substances. Their use is getting more popular, and display systems offer ... Libraries of polypeptides displayed on the surface of bacteria can be screened using flow cytometry or iterative selection ...
I their method polyethyleneglycol (PEG) was used as soluble support in S&P synthesis of peptide libraries. MeO-CH2-CH2-O-(CH2- ... The split and pool synthesis was first applied to prepare peptide libraries on solid support. The synthesis was realized in a ... Two libraries are synthesized. A template library containing at one end one of the BBs and its code followed by two annealing ... On the other hand, the presence of all possible structural varieties in a library assures that the library is a combinatorial ...
Peptide+Elongation+Factor+1 at the US National Library of Medicine Medical Subject Headings (MeSH). ...
Phage display is a different use of phages involving a library of phages with a variable peptide linked to a surface protein. ... providing a link between the peptide variant and its encoding gene. Variant phages from the library can be selected through ... Phage proteins often have antimicrobial activity and may serve as leads for peptidomimetics, i.e. drugs that mimic peptides.[31 ... thus allowing them to retrieve the peptides encoded in them for further study.[30] ...
Receptors,+Vasoactive+Intestinal+Peptide на US National Library of Medicine Medical Subject Headings (MeSH) ...
Plant, 145, 5-17 (2012). Schilling O., Overall C.M. Proteome-derived, database-searchable peptide libraries for identifying ... Beginning with a peptide library generated from endopeptidase digestion of a proteome, this technique allows for screening and ... Protease Specificity Profiling by Tandem Mass Spectrometry Using Proteome-derived Peptide Libraries. Gel-Free Proteomics: ... 21, 566-569 (2003). Chen S.-H., Chen C.-R., Chen S.-H., Li D.-T., Hsu J.-L. Improved N(α)-acetylated peptide enrichment ...
"Characterization of three forms of rabbit microsomal cytochrome P-450 by peptide mapping utilizing limited proteolysis in ... Wiley Classics Library. ISBN 0471303097. http://books.google.com/books/about/Enzyme_Kinetics.html?id=T60oAAAAYAAJ. ...
In this system, particular proteins are targeted for destruction by the ligation of at least four copies of a small peptide ... Muscular atrophy at the US National Library of Medicine Medical Subject Headings (MeSH). ...
U.S. National Library of Medicine. Retrieved 7 June 2020.. *^ Zhanel G, Critchley I, Lin LY, Alvandi N (January 2019). " ... The use of antimicrobial peptides against C. acnes is under investigation as a treatment for acne to overcoming antibiotic ... sebaceous gland cells produce more antimicrobial peptides, such as HBD1 and HBD2, in response to the binding of TLR2 and TLR4.[ ...
proapoptotic peptide against ANXA2 and prohibitin (Adipotide). *exotoxin against IL-2 (Denileukin diftitox) ... U.S. National Library of Medicine: Drug Information Portal - Rituximab. *v. *t ...
"National Center for Biotechnology Information, U.S. National Library of Medicine.. *^ a b "Entrez Gene: zona pellucida ... The nascent protein contains a N-terminal signal peptide sequence, a conserved "ZP domain" module, a consensus furin cleavage ... This article incorporates text from the United States National Library of Medicine, which is in the public domain. ... "National Center for Biotechnology Information, U.S. National Library of Medicine.. *^ "Mouse PubMed Reference:". ...
48: 1-9. doi:10.1016/j.peptides.2013.07.024. PMID 23933301.. *^ Berger M, Neth O, Ilmer M, Garnier A, Salinas-Martín MV, de ... "Fibromyalgia Library. ProHealth.com. Retrieved 2008-11-01.. *Fight Club for Flies video, Science Take, New York Times, February ... Substance P (SP) is an undecapeptide (a peptide composed of a chain of 11 amino acid residues) member of the tachykinin ... Yip J, Chahl LA (Apr 2001). "Localization of NK1 and NK3 receptors in guinea-pig brain". Regulatory Peptides. 98 (1-2): 55-62. ...
U.S. National Library of Medicine: Drug Information Portal - Levothyroxine. *v. *t ... Peptide YY. Liver/other. *Insulin-like growth factor *IGF-1. *IGF-2 ...
Subscription or UK public library membership required.) *^ Newlands, John A. R. (20 August 1864). "On Relations Among the ... Solid state crystal structures have been determined for many complexes of alkali metal ions in small peptides, nucleic acid ...
... s at the US National Library of Medicine Medical Subject Headings (MeSH) ...
This article incorporates public domain text from The U.S. National Library of Medicine and the National Institute of ... Deficiencies of intracellular signaling peptides and proteins. GTP-binding protein regulators. GTPase-activating protein. * ...
Marise A. Hussey, Anne Zayaitz Endospore Stain Protocol Microbe Library (American Society of Microbiology) ... subtilis spores are useful for the expression of recombinant proteins and in particular for the surface display of peptides and ... "Ethylene Oxide Sterilization , Disinfection & Sterilization Guidelines , Guidelines Library , Infection Control ,CDC". www.cdc. ...
Peptides. 31 (8): 1441-8. doi:10.1016/j.peptides.2010.04.021. PMC 2905501 . PMID 20441784. Schein CH, Volk DE, Oezguen N, Paul ... J Invertebr Pathol Additional material "Picornaviridae". Medical Subject Headings (MeSH). National Library of Medicine. ... Peptides. 27 (7): 1676-84. doi:10.1016/j.peptides.2006.01.018. PMC 1629084 . PMID 16540201. Schein CH, Oezguen N, van der Heden ... Schein CH, Oezguen N, Volk DE, Garimella R, Paul A, Braun W (July 2006). "NMR structure of the viral peptide linked to the ...
... s at the US National Library of Medicine Medical Subject Headings (MeSH) ...
Ooi, C. P.; Yassin, Z; Hamid, T. A. (2012). "Momordica charantia for type 2 diabetes mellitus". The Cochrane Library. 8 (8): ... "Gene cloning and expression of a novel hypoglycaemic peptide from Momordica charantia". Journal of the Science of Food and ...
... site for larger peptide ligands is usually located in the extracellular domain whereas the binding site for smaller non-peptide ... Cell+surface+receptors at the US National Library of Medicine Medical Subject Headings (MeSH) ... and can be a protein or peptide (short protein), or another small molecule such as a neurotransmitter, hormone, pharmaceutical ...
"National Center for Biotechnology Information, U.S. National Library of Medicine.. *^ a b c d "Entrez Gene: hydroxyacyl- ... HADHB encodes a 51.2 kDa protein that is composed of 474 amino acids; 124 peptides have been observed through mass spectrometry ... This article incorporates text from the United States National Library of Medicine, which is in the public domain. ... "National Center for Biotechnology Information, U.S. National Library of Medicine.. .mw-parser-output cite.citation{font-style: ...
This article incorporates text from the United States National Library of Medicine, which is in the public domain. ... Wikipedia articles incorporating text from the United States National Library of Medicine ...
U.S. National Library of Medicine.. *↑ Ruhs, Stefanie; Nolze, Alexander; Hübschmann, Ralf; Grossmann, Claudia (July 2017). "30 ... amines an peptide or protein hormones) are watter-soluble an act on the surface o target cells via seicont messengers; steroid ... peptides, an proteins). The glands that secrete hormones comprise the endocrine seegnalin system. The term hormone is whiles ...
"Florida Atlantic University Libraries. Retrieved 2010-03-31.. *↑ "Leonid Hurwicz (1917-2008)". Jewish Virtual Library. American ... "for the development of radioimmunoassays of peptide hormones"[۶۳] ۱۹۷۸ دنیل ناتانس[۱] United States "for the discovery of ... "for their discoveries concerning the peptide hormone production of the مغز"[۶۳] ...
National Library of Medicine (NLM) - National Prevention Information Network (NPIN) - natural history study - natural killer ... peptide - perianal - perinatal - perinatal transmission - peripheral neuritis - peripheral neuropathy - persistent generalized ...
The Cochrane Library. 8 (8): CD007845. doi:10.1002/14651858.CD007845.pub3. PMID 22895968.. ... "Gene cloning and expression of a novel hypoglycaemic peptide from Momordica charantia". Journal of the Science of Food and ...
These cells have T-cell receptors and CD4 molecules that, in combination, bind antigenic peptides presented on major ... Leukocytes at the US National Library of Medicine Medical Subject Headings (MeSH) ...
"National Center for Biotechnology Information, U.S. National Library of Medicine.. .mw-parser-output cite.citation{font-style: ... Pituitary adenylate cyclase-activating peptide (PACAP). *Pleiotrophin. *Renalase. *Thrombopoietin (see here instead) ... "National Center for Biotechnology Information, U.S. National Library of Medicine.. *^ https://www.sigmaaldrich.com/catalog/ ...
Autocrine signaling at the US National Library of Medicine Medical Subject Headings (MeSH) "Autocrine versus juxtacrine ... when a T cell is induced to mature by binding to a peptide:MHC complex on a professional antigen-presenting cell and by the B7: ...
1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1-2): 149- ... "A cDNA encoding the calcitonin gene-related peptide type 1 receptor". J Biol Chem. 271 (19): 11325-9. doi:10.1074/jbc.271.19. ...
Esterase activity against N-acylated peptide ester substrates". Arch. Biochem. Biophys. 165: 72-79. PMID 4441086. ... Wiley Classics Library. ISBN 0471303097. http://books.google.com/books/about/Enzyme_Kinetics.html?id=T60oAAAAYAAJ. ...
California State Library. ISBN 978-1-58703-133-5. .. *^ "Mold: A Health Hazard (Release #1605-096)". FEMA. November 8, 2005. ... The common house mold, Trichoderma longibrachiatum, produces small toxic peptides containing amino acids not found in common ...
This article incorporates text from the United States National Library of Medicine, which is in the public domain. ... CD30+Antigens at the US National Library of Medicine Medical Subject Headings (MeSH) ... Wikipedia articles incorporating text from the United States National Library of Medicine ... Signaling peptide/protein receptor modulators. Growth factor receptor modulators. Retrieved from "https://en.wikipedia.org/w/ ...
Retrieved August 12, 2016 - via Open Library.. *^ a b Pauling L (1976). Vitamin C, the Common Cold, and the Flu. W.H. Freeman ... This increases peptide hormone stability and activity.[99][100]. Pharmacokinetics[edit]. Absorption[edit]. From the U.S. ... United States National Library of Medicine. Archived from the original on May 5, 2009.. ... Eipper BA, Stoffers DA, Mains RE (1992). "The biosynthesis of neuropeptides: peptide alpha-amidation". Annual Review of ...
"US National Library of Medicine. Retrieved 2015-07-30.. *^ a b c d e f g h i Schumacher, Ton N.; Schreiber, Robert D. (April 3 ... The nature of the central TCR-exposed residues of MHC-bound peptides is associated with peptide immunogenicity.[15] ... Furthermore, for a peptide to induce an immune response (activation of T-cells by antigen-presenting cells) it must be a large ... For human tumors without a viral etiology, novel peptides (neo-epitopes) are created by tumor-specific DNA alterations.[15] ...
NISTs new CHO peptide library will enable better production of treatments for psoriasis, cancer, hemophilia and leukemia. ... As more information concerning contaminants is discovered and then added to the peptide library, the utility of the library ... known as a peptide library, was created by NIST researchers. The data used in the library was gathered with help from other ... This new CHO tool is now available to the public and adds to a growing list of peptide libraries in the NIST Mass Spectrometry ...
Now, the mechanisms employed by entomopathogenic bacteria for the biosynthesis of a large family of bioactive peptides have ... Here we report mechanisms leading to the biosynthesis of the chemically diverse rhabdopeptide/xenortide peptides (RXPs). They ... the reactions responsible for generating such diverse chemical libraries are often not clear. ... little is known about the mechanisms generating such chemically diverse libraries. ...
... mdp96tab at shef.ac.uk mdp96tab at shef.ac.uk Mon Apr 2 10:33:46 EST 2001 *Previous message: Random ... So my question is this, do any of you out there have a small amount of a phage based random peptide library that youd be ... Ive been using a couple of commercially available libraries (7mer to 12mer) to look for peptide sequences which bind cell ... Dear All, Im mailing to request further information and possible donations of random peptide libraries. ...
... Takashi Kawakami and Hiroshi Murakami Department of Life Sciences, ... R. Odegrip, D. Coomber, B. Eldridge et al., "CIS display: in vitro selection of peptides from libraries of protein-DNA ... N. Muranaka, T. Hohsaka, and M. Sisido, "Four-base codon mediated mRNA display to construct peptide libraries that contain ... R. W. Roberts and J. W. Szostak, "RNA-peptide fusions for the in vitro selection of peptides and proteins," Proceedings of the ...
The PEPscreen peptide library is configured in a convenient plate format, perfect for screening applications, epitope mapping ... Affordable and fast delivery of custom peptide libraries for target identification. ... Peptide Library Design. We offer complimentary peptide library design services for overlapping peptide fragment libraries, ... Applications Using Peptide Libraries. *Overview of applications using peptide libraries. *Diverse CD4 Response to Vaccinia ...
Peptide Based Capture Probes Investigators. Mello, Charlene; Arcidiacono, Steve. Institutions. US Army - Soldier and Biological ... To integrate peptide-based detection into existing biosensors to detect the presence of food pathogens.. Funding Source. United ...
... peptide library and would like to know the experience of those that have used constrained peptides vs unconstrained peptides. ... constrained random peptide libraries. Scott Vande Pol sbv at pop.cwru.edu Tue Aug 12 10:40:29 EST 1997 *Previous message: Ion- ... on the constrained library offset by the potential lack of function when the peptides are subsequently used unconstrained? -- ... Are peptides that are removed from their constrained vectors likely to still function in an unconstrained context? ie is the ...
... a brain and gastrointestinal peptide by Charles B. Nemeroff, 1982, New York Academy of Sciences edition, in English ... Open Library is an initiative of the Internet Archive, a 501(c)(3) non-profit, building a digital library of Internet sites and ... Edited by Open Library Bot Linked existing covers to the edition. June 8, 2009. Edited by ImportBot Found a matching record ... Open Library. OL3504284M Internet Archive. neurotensinbrain0400unse ISBN 10. 0897661915, 0897661907 LC Control Number. 82024692 ...
Epitope mapping using randomly generated peptide libraries.. Bongartz J1, Bruni N, Or-Guil M. ... Here, we present a method comprising binding studies of serum antibody pools to synthetic random peptide libraries, and data ... National Center for Biotechnology Information, U.S. National Library of Medicine 8600 Rockville Pike, Bethesda MD, 20894 USA ... Due to the design of the library and the sophisticated analysis, the method is able to classify and predict the different mice ...
... and utility of peptide libraries. Chapter focus on methods and techniques for synthesis, genetic expression, ... combinatorial peptide cyclic peptides isopeptide linkages library synthesis peptide libraries peptidomimetic libraries ... Peptide and Peptide Library Cyclization via Bromomethylbenzene Derivatives David E. Hacker, Mohammed Almohaini, Aruna ... Screening Peptide Array Library for the Identification of Cancer Cell-Binding Peptides ...
The peptide is followed by a short spacer (Gly-Gly-Gly-Ser) and then the wild-type pIII sequence. The library consists of ... The displayed peptide is expressed at the N-terminus of pIII, i.e., the first residue of the mature protein is the first ... C7C Phage Display Peptide Library is based on a combinatorial library of random peptides fused to a minor coat protein (pIII) ... Ph.D.™-C7C Phage Display Peptide Library Kit The Ph.D.™-C7C Phage Display Peptide Library Kit contains a tube of the Ph.D.-C7C ...
Select a link on our interactive display for descriptions and publications for the highlighted peptide library applications. ... Several applications in the area of drug design and drug delivery utilize peptide libraries. ... PEPscreen peptide libraries have been used to study structural properties of proteins such as Human Thrombin.. Kasetty et. al. ... Several applications in the area of drug design and drug delivery utilize peptide libraries. Select a link on our interactive ...
A site was chosen for ligation and syntheses of the appropriate peptide segments were attempted using solid phases peptide ... The conditions used in both reactions are suited to use on protected peptides. Model studies were performed on a range of ... Presented is a novel protocol for the ligation of protected peptide segments that relies on a selective ruthenium-catalyzed ...
The library was constructed so that experimental peptide tandem mass spectra could be compared with those in the library, ... Using annotated peptide mass spectrum libraries for protein identification.. Craig R1, Cortens JC, Fenyo D, Beavis RC. ... A system for creating a library of tandem mass spectra annotated with corresponding peptide sequences was described. This ... The library spectra were created by averaging together spectra that were annotated with the same peptide sequence, sequence ...
Fragmentation Pathways of Protonated Peptides. Authors. *. Bela Paizs,. *Dtsch. Krebsforschungszent., D-69120 Heidelberg, ...
... Nature. 1996 Mar 28;380(6572):364-6. doi: 10.1038/380364a0. ... Peptides capable of mediating selective localization of phage to brain and kidney blood vessels were identified, and showed up ... One of the peptides displayed by the brain-localizing phage was synthesized and shown to specifically inhibit the localization ... These peptide sequences represent the first step towards identifying selective endothelial markers, which may be useful in ...
Purely theoretical/review papers should provide new insight into the principles of protein/peptide structure and function. ... Protein & Peptide Letters. Volume 28, 12 Issues, 2021. ISSN: 1875-5305 (Online). ISSN: 0929-8665 (Print). This journal supports ... Protein & Peptide Letters was introduced in 2001 with the assistance of Dr. Ben M. Dunn, who is the current Editor-in-Chief ... Advances in Protein and Peptide Sciences. You can view the contents of this eBook [here] or purchase it [here].. ...
Overlapping Peptide Library Overlapping peptide library is generated by breaking the original protein or peptide into many ...
We developed a peptide library spanning the entire Spike glycoprotein from SARS-CoV-2. The library is provided in a convenient ... COVID-19 Peptides , 96-well Overlapping Peptide Library; Specifications:

Spike glycoprotein ... 9aa for the C-terminal peptide.

Gross peptide weight: not quantified

Synthesis scale ... of peptides

Coronavirus Spike glycoproteins mediate the virus attachment to host cell surface receptors and facilitate ...
Peptide puts mouse arthritis out of joint.(indication that vasointestinal peptide can reduce inflamation and damage caused by ... rheumatoid arthritis, Brief Article) by Science News; Science and technology, general Peptides Research Rheumatoid arthritis ... APA style: Peptide puts mouse arthritis out of joint.. (n.d.) >The Free Library. (2014). Retrieved Sep 22 2018 from https://www ... MLA style: "Peptide puts mouse arthritis out of joint.." The Free Library. 2001 Science Service, Inc. 22 Sep. 2018 https://www. ...
If you already have a Wiley Online Library or Wiley InterScience user account: login above and proceed to purchase the article. ...
Xenome Ltd Licenses Peptides from xdiscoverTM Library to MedImmune - read this article along with other careers information, ... has exercised its option to obtain exclusive licenses to four peptides from Xenomes xdiscover™ venom peptide library against ... The xdiscover™ library is a proprietary collection of more than 2000 primarily venom-derived peptides and is the result of a ... Xenome Ltd Licenses Peptides from xdiscoverTM Library to MedImmune. Published: Mar 23, 2010 ...
Peptide Science covers a field by discussing research from the leading laboratories in a field and should pose questions for ... Current Protein & Peptide Science. Volume 21, 12 Issues, 2020. ISSN: 1875-5550 (Online). ISSN: 1389-2037 (Print). This journal ... Current protein and Peptide Science was introduced in 2000 with the assistance of Dr. Ben M. Dunn, who is the current Editor-in ... Advances in Protein and Peptide Sciences. You can view the contents of this eBook [here] or purchase it [here].. ...
Encephalin is a small protein molecule made up of five peptides (a pentapeptide). These peptides are in turn made up of amino ... Encephalin is a small protein molecule made up of five peptides (a pentapeptide). These peptides are in turn made up of amino ... Credit: ALFRED PASIEKA/SCIENCE PHOTO LIBRARY. Caption: Encephalin. Computer molecular graphic of part of a molecule of ... peptide, polypeptide, proteomics, round shape, rounded circular, structure ...
Encephalin is a small protein molecule made up of five peptides (pentapeptide). Peptides are in turn made up of amino acids. ... Encephalin is a small protein molecule made up of five peptides (pentapeptide). Peptides are in turn made up of amino acids. ... Keywords: analgesic, artwork, autoradiogram, biochemical, biochemistry, brain peptide, chemical, chemistry, circle circles, ... Credit: ALFRED PASIEKA/SCIENCE PHOTO LIBRARY. Caption: Encephalin. Computer molecular graphic of part of a molecule of ...
... such as an overlapping peptide library and random peptide library. ... Use GenScripts peptide library design tools to generate peptide libraries, ... Overlapping Peptide Library :. Overlapping peptide library can be used in epitope mapping. The peptide library generation ... Please specify the library size (number of peptides within the library, maximum 50) : Scrambled Library:. Scramble Library is ...
A team led by Technical University Munich researcher Bernhard Kuster has built a reference library containing more than 200,000 ... synthetic peptides and phosphopeptides that they said will enable the development, improvement, and evaluation of a wide ... The peptide library "is an incredibly useful resource," Paul Rudnick, a researcher at the National Institute of Standards and ... The synthetic peptide library generated by Kuster and his colleagues allows researchers to evaluate this assumption and the ...
In this work we varied in a combinatorial fashion the ring sizes of bicyclic peptides in phage libraries, expecting that they ... Phage selections with combinatorial libraries of uniformly sized bicyclic peptides have recently yielded potent and selective ... peptide. libraries of the format Cys-(Xaa)m-Cys-(Xaa)n-Cys (Xaa are random amino acids. , m and n = 3, 4, 5 or 6) were ... Phage display libraries of differently sized bicyclic peptides Inmaculada Rentero Rebollo,a Alessandro Angelinia and Christian ...
A peptide library is a tool for protein-related study. A peptide library contains a great number of peptides that have a ... peptide library is synthesized on solid phase, mostly on resin, which can be made as flat surface or beads. The peptide library ... having the library of different peptides at residue 2 and 3 would let you see if some change in chemical properties in the N- ... As a general formula, if you made a completely random peptide library with n amino acids for each link in the chain with a ...
The synthetic peptides all exhibited significant biological activities. Medusins were a novel family of antimicrobial peptides ... These discoveries of novel peptides from amphibian skin secretions have enriched our knowledge of bioactive peptides from this ... Bradykinin-like peptides (BRPs) exhibited significant and selective activities on a range of mammalian smooth muscle ... In the course of this research, genomic and proteomic techniques have been used to investigate the bioactive peptides from the ...
  • I've been using a couple of commercially available libraries (7mer to 12mer) to look for peptide sequences which bind cell surface receptors. (bio.net)
  • Alanine Scanning Library - Systematically substitute each amino acid position on your peptide sequences with alanine- the smallest of the naturally occurring amino acids that maintains it chirality. (sigmaaldrich.com)
  • The library consists of approximately 1 x 10 9 electroporated (i.e., unique) sequences. (neb.com)
  • The library consists of approximately 10 9 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage. (neb.com)
  • A system for creating a library of tandem mass spectra annotated with corresponding peptide sequences was described. (nih.gov)
  • Here we report a new approach to studying organ-selective targeting based on in vivo screening of random peptide sequences. (nih.gov)
  • These peptide sequences represent the first step towards identifying selective endothelial markers, which may be useful in targeting cells, drugs and genes into selected tissues. (nih.gov)
  • Xenome has leveraged the identification of venom peptide sequences into high quality synthesised peptides arrayed into a library suitable for high throughput screening. (biospace.com)
  • The researchers "were actually happy to see that the published [identification] algorithms do an OK job," Kuster said, noting that their findings demonstrated they struggled most in conclusively identifying peptide sequences where they are very similar to other sequences - an issue that he said was already "acknowledged in the field. (genomeweb.com)
  • Affinity selections against the tumor-associated serine protease urokinase-type plasminogen activator yielded bicyclic peptide inhibitors with a large variety of consensus sequences. (rsc.org)
  • Several of the identified consensus sequences were exclusively found in bicyclic peptides having defined ring size combinations. (rsc.org)
  • As a general formula, if you made a completely random peptide library with n amino acids for each link in the chain with a length of x, the total number of possible sequences is n^x. (wikipedia.org)
  • A library of overlapping peptide sequences of specific length and specific offset, to cover the entire native protein sequence is designed in this strategy and is used for linear, continuous epitope mapping and T-cell epitope determination. (scientistsolutions.com)
  • Suited for all protease classes except carboxyproteases and those aminoproteases and dipeptidases requiring a free α-amine for cleavage, PICS simultaneously profiles the specificity of prime and nonprime positions and directly determines scissile peptide bonds of up to hundreds of cleavage site sequences in a single experiment. (springer.com)
  • Cationic import signal (IS) sequences and the nuclear localization sequence (NLS) from SV40 large T-antigen were incorporated at the N-terminus of the hp53 tet peptides. (openlibrary.org)
  • To demonstrate that the additional regions identified were biologically significant, peptides mimicking the gp91-phox sequences F77LRGSSACCSTRVRRQL93 and E451WFADLLQLLESQ463 were synthesized and assayed for their ability to inhibit NADPH oxidase activity. (pnas.org)
  • Recently, dynamic peptide libraries (DPLs) have been proposed to select self-assembling materials from dynamically exchanging mixtures of dipeptide inputs in the presence of a nonspecific protease enzyme, where peptide sequences are selected and amplified based on their self-assembling tendencies. (rsc.org)
  • Affinity selection on Nipah Virus-neutralizing monoclonal antibodies using random sequence peptide libraries on MS2 VLPs selected peptides with sequence similarity to peptide sequences found within the envelope glycoprotein of Nipah itself, thus identifying the epitopes the antibodies recognize. (osti.gov)
  • The selected peptide sequences themselves are not necessarily identical in all respects to a sequence within Nipah Virus glycoprotein, and therefore may be referred to as epitope mimics VLPs displaying these epitope mimics can serve as vaccine. (osti.gov)
  • Recently in the Link Lab, a precursor genome mining approach to find novel lasso peptides from the known genomic sequences of bacteria had been shown accurate for a normal, Class II lasso peptide, astexin-1. (princeton.edu)
  • Instead of beginning with a glycine or cysteine amino acid, as most lasso peptide sequences do, the proposed lasso peptide begins with a serine amino acid and could have different characteristics than other lasso peptides. (princeton.edu)
  • Three different DNA sequences of the proposed C. crescentus lasso peptide gene cluster were expressed under different conditions and tested for lasso peptide production: a wild-type, a negative control, and a no hairpin construct to increase peptide production. (princeton.edu)
  • We describe here a conceptually unique set of individual synthetic peptide combinatorial libraries (SPCLs), termed a positional scanning SPCL (PS-SPCL), that can be used for the rapid (i.e., a single day) identification of peptide sequences that bind with high affinity to antibodies, receptors or other acceptor molecules. (nih.gov)
  • Peptide libraries are ideal for discovering novel therapeutic strategies because they display hundreds of peptide combinations in parallel, grossly decreasing the time and effort required to identify target sequences. (genscript.com)
  • The technology, designed to specifically bind and enter tumor cells and the vasculature supporting tumors, is thought to be the world's largest library of human-derived targeting agents, representing thousands of unique peptide sequences. (nanowerk.com)
  • The work that Drs. Pasqualini and Arap have done is truly revolutionary in the sheer number of targeting sequences and new receptors that have been discovered, the exquisite specificity of many of the peptides, and the fact that these were generated in human patients rather than experimental animal models," said Christopher Anzalone, Arrowhead's CEO. (nanowerk.com)
  • Specific peptide sequences are synthesized directly on encoded beads with a 1:1 relationship between peptide sequence and embedded code, thereby making it possible to track many peptide sequences throughout the course of an experiment within a single small volume. (spie.org)
  • The design tools below will guide you through the steps of isolating minimum length active peptide sequences, identifying critical amino acid residues, designing analogs for sequence optimization and will provide indicative pricing for all peptide sets. (mimotopes.com)
  • A phage display library was used to identify peptide sequences that target bone. (jove.com)
  • Using a series of ERα-mutants, we found that these three classes of peptides showed different interaction patterns from each other, suggesting that not all LXXLL motifs are the same and that receptor binding selectivity can be achieved by altering sequences flanking the LXXLL core motif. (asm.org)
  • Many integrins recognize short RGD-containing amino acid sequences and such peptide sequences can be identified from phage libraries by panning with an integrin. (rupress.org)
  • Here, in a reverse strategy, we have used such libraries to isolate minimal receptor sequences that bind to fibronectin and RGD-containing fibronectin fragments in affinity panning. (rupress.org)
  • In here, we demonstrate the discovery and optimization of aqueous, gel-phase nanostructures based on dynamically exchanging peptide sequences that self-select to maximize charge transfer of n-type semiconducting naphthalenediimide (NDI)-dipeptide bioconjugates with various π-electron-rich donors (dialkoxy/hydroxy/amino-naphthalene or pyrene derivatives). (brad.ac.uk)
  • The purification of the peptide mixture containing N-biotinylated sequences was performed by affinity chromatography on an avidin-agarose column. (cnrs-orleans.fr)
  • Use our free and easy-to-use PepSequencer for generation of overlapping peptide sequences! (jpt.com)
  • Peptide libraries are large collections of related and diverse sequences used to identify critical motifs for protein function or protein: protein recognition. (neobiolab.com)
  • Many of the peptides aligned with varying homology to the serine protease sequence which demonstrated that the antibodies were selecting specific sequences from the libraries rather than just random peptides. (edu.au)
  • These displaying phages can then be screened against other proteins, peptides or DNA sequences, in order to detect interaction between the displayed protein and those other molecules. (wikipedia.org)
  • Phage display was first described by George P. Smith in 1985, when he demonstrated the display of peptides on filamentous phage (long, thin viruses that infect bacteria) by fusing the virus's capsid protein to one peptide out of a collection of peptide sequences. (wikipedia.org)
  • Applications of phage display technology include determination of interaction partners of a protein (which would be used as the immobilised phage "bait" with a DNA library consisting of all coding sequences of a cell, tissue or organism) so that the function or the mechanism of the function of that protein may be determined. (wikipedia.org)
  • Sieber, S. A. & Marahiel, M. A. Molecular mechanisms underlying nonribosomal peptide synthesis: approaches to new antibiotics. (nature.com)
  • Chapter focus on methods and techniques for synthesis, genetic expression, hybrid synthesis-expression, examples of modern utility of these libraries, de novo discovery of reactions, hybrid organic-inorganic materials and, emerging tools for the analysis of these libraries by method of genetic selection and next-generation sequencing. (springer.com)
  • A site was chosen for ligation and syntheses of the appropriate peptide segments were attempted using solid phases peptide synthesis techniques and an Fmoc protection approach. (bl.uk)
  • The usefulness of this form of peptide synthesis is limited as you can't go beyond 70 acids in length. (wikipedia.org)
  • The synthesis of peptide mixture libraries using pre-mixed solutions of amino acids was introduced to overcome the limitations of OBOC approach to make larger libraries. (scientistsolutions.com)
  • These powerful methods-often detailed here by their pioneers-include protocols for the chemical synthesis of peptide libraries, for constructing peptide libraries that are displayed on the surface of filamentous phage or bacteria, and for the rapid screening of these libraries for molecules with biospecific properties. (springer.com)
  • For peptide synthesis ~2.4% of the peptides submitted, due to difficult sequence were unable to be synthesized. (thermofisher.com)
  • This paper reports the synthesis and screening of a combinatorial peptide library for new affinity ligands for glycosylated haemoglobin (HbA(1c)), which is an important indicator of diabetes control. (diva-portal.org)
  • We offer custom peptide libraries synthesis in 96-wells microplate formats. (genosphere-biotech.com)
  • Whether you intend to screen peptides for enzyme substrate profiling, elucidate substrate specificities, protein-protein interaction region mapping, antibody epitope mapping, we provide cost-effective fmoc-based simultaneous multi-peptide synthesis technologies to fit your needs. (genosphere-biotech.com)
  • Herein we present a method for the combinatorial synthesis and screening of large one-bead-one-compound (OBOC) libraries of cyclic peptides against biological targets such as proteins. (osu.edu)
  • Up to ten million different cyclic peptides are rapidly synthesized on TentaGel microbeads by the split-and-pool synthesis method and subjected to a multistage screening protocol which includes magnetic sorting, on-bead enzyme-linked and fluorescence-based assays, and in-solution binding analysis of cyclic peptides selectively released from single beads by fluorescence anisotropy. (osu.edu)
  • Peptide Libraries-Genemed Synthesis Inc. (genemedsyn.com)
  • Researchers led by the Technical University of Munich (TUM) report on the synthesis of a library of more than 330,000 reference peptides representing essentially all canonical proteins of the human proteome. (technologynetworks.com)
  • In a manuscript published online in Nature Methods, ProteomeTools scientists report on the synthesis of a library of more than 330,000 reference peptides (termed PROPEL for ProteomeTools Peptide Library) representing essentially all canonical proteins of the human proteome. (technologynetworks.com)
  • Peptide libraries are synthesized on Mimotopes' unique proprietary synthesis platform and are typically supplied at the 1 to 3 mg scale for fast, efficient screening work. (mimotopes.com)
  • The proportions of each group and subgroup at individual peptide positions were changed in the library synthesis, and the effect of these changes on CTL activity was measured in a sensitive RMA-S cell assay. (rupress.org)
  • During peptide library synthesis, by means of the single-resin method in which coupling on variable positions is carried out using an equimolar mixture of amino acids, biotin was used to cap the unreacted amino groups remaining after coupling of the equimolar amino acid mixture. (cnrs-orleans.fr)
  • NovoPro has a rapid high-throughput parallel peptide synthesis platform that enables our customers to synthesize larger quantities of high purity peptides at lower cost than traditional methods. (novoprolabs.com)
  • In a final study, we report on the design and synthesis of a regiospecific, kinetically controlled, bis-electrophile for cyclizing phage-displayed peptides. (tamu.edu)
  • Maximized, uniform & extensive randomization of peptides ranging between 8 to 30 amino acids achieved by NNK, Split-mix-split synthesis or Tri-mer codon strategies. (rxbiosciences.com)
  • At NeoScientific we use a unique solid phase synthesis approach to produce highly targeted and complex libraries of the highest quality. (neobiolab.com)
  • A rapid high-throughput parallel peptide synthesis platform that enables to synthesize a larger quantity of high purity peptides. (abclonalbio.com)
  • having the library of different peptides at residue 2 and 3 would let you see if some change in chemical properties in the N-terminal tail of the ligated protein make the protein more useful or useful in a different way. (wikipedia.org)
  • Combinatorial peptide libraries displayed on phage have allowed rapid screening of billions of different peptides against a wide variety of target molecules (reviewed in 1-3). (neb.com)
  • A peptide library with a diversity of ~ 4.7X1011 different peptides was constructed containing both linear and disulfide-constrained peptides. (universityofcalifornia.edu)
  • A peptide library is a systematic combination of a large number of different peptides. (novoprolabs.com)
  • This displayed the different peptides on the outer surfaces of the collection of viral clones, where the screening step of the process isolated the peptides with the highest binding affinity. (wikipedia.org)
  • The PEPscreen peptide library is configured in a convenient plate format, perfect for screening applications, epitope mapping and peptide microarray production. (sigmaaldrich.com)
  • A peptide library constructed using positional scanning can be very informative in studying protein interfaces whether in epitope mapping or protein-protein interaction. (sigmaaldrich.com)
  • Epitope mapping using randomly generated peptide libraries. (nih.gov)
  • Overlapping peptide library is generated by breaking the original protein or peptide into many equal-length overlapping fragment and it is used for linear, continuous epitope mapping and T-cell epitope determination. (biosyn.com)
  • Overlapping peptide library can be used in epitope mapping. (genscript.com)
  • As a general guideline, shorter peptides are easier to synthesize but they have less chances for multiple epitope hits. (genscript.com)
  • Note: for most T cell epitope mapping applications, choose 11-mers as starting points for your peptide pools. (genscript.com)
  • The truncation library is used to predict the minimum amino acid length required for optimum epitope activity. (scientistsolutions.com)
  • The generation of peptide library in which alanine (Ala, A) is systematically substituted into each of the amino in the identified epitope. (scientistsolutions.com)
  • Over the last ten years, the Ph.D. libraries from New England Biolabs have become the dominant tools in this field, with hundreds of publications describing applications including epitope mapping (4-10), mapping protein-protein contacts (11-16) and identification of peptide mimics of non-peptide ligands (17,18). (neb.com)
  • These libraries are fully customizable and contain an acetate counter ion to avoid potential toxicity in epitope discovery and mapping. (thermofisher.com)
  • PEPotec immuno peptide libraries are fully customizable and supplied with acetate as the counterion to avoid potential toxicity issues in epitope discovery and mapping. (thermofisher.com)
  • Epitope prediction based on random peptide library screening has become a focus as a promising method in immunoinformatics research. (mdpi.com)
  • Using this benchmark dataset and a representative dataset, five examples of the most popular epitope prediction software products which are based on random peptide library screening have been evaluated. (mdpi.com)
  • Sun P, Chen W, Huang Y, Wang H, Ma Z, Lv Y. Epitope Prediction Based on Random Peptide Library Screening: Benchmark Dataset and Prediction Tools Evaluation. (mdpi.com)
  • As a general guideline, shorter peptides have less chances for multiple epitope hits. (genemedsyn.com)
  • The PEPotec Immuno Custom Peptide Libraries are designed for use in a variety of immunological applications, including epitope mapping of B- and T-cells, vaccine development and screening of peptide vaccines. (technologynetworks.com)
  • Immunization of syngeneic mice with this peptide elicited CTL that lysed EL4 cells as well as RMA-S cells pulsed with peptides isolated from Db molecules of EL4 cells, indicating functional similarity between the mimicking peptide and the naturally processed CTL epitope. (rupress.org)
  • It is widely used in peptide library screening for drug discovery, target validation, epitope mapping, vaccine development, and structure-activity studies. (novoprolabs.com)
  • The optimal peptide length for epitope mapping is 8 to 20 amino acids with an offset of 2-4 amino acids. (jpt.com)
  • Overlapping Library - Overlapping peptide libraries are ideal for T-cell epitope identification because T cell epitopes are by their nature short linear peptides from the primary protein sequence. (abclonalbio.com)
  • so far I have been unsuccessful in locating a suitable sequence and am hoping to broaden my search by using a panel of libraries. (bio.net)
  • Once the essential amino acid residues for protein activity are identified by alanine scanning, the direction of truncation can be selected around these residues, rather than removing residues from both ends of the peptide sequence. (sigmaaldrich.com)
  • Positional Scanning Library - Optimize the peptide lead sequence. (sigmaaldrich.com)
  • The substitution can be performed on a single or multiple positions on a peptide sequence. (sigmaaldrich.com)
  • The peptide is followed by a short spacer (Gly-Gly-Gly-Ser) and then the wild-type pIII sequence. (neb.com)
  • The library spectra were created by averaging together spectra that were annotated with the same peptide sequence, sequence modifications, and parent ion charge. (nih.gov)
  • The library was constructed so that experimental peptide tandem mass spectra could be compared with those in the library, resulting in a peptide sequence identification based on scoring the similarity of the experimental spectrum with the contents of the library. (nih.gov)
  • A software implementation that performs this type of library search was constructed and successfully used to obtain sequence identifications. (nih.gov)
  • Please input your protein or peptide sequence here! (genscript.com)
  • Truncation Library can be used to identify the shortest amino acid sequence needed for the peptide activity. (genscript.com)
  • Positional Scanning Library is one of the key methods for sequence optimization. (genscript.com)
  • Used in sequence optimization, Random Library is similar to positional scanning library. (genscript.com)
  • Scramble Library is constructed by carrying out permutation on the original peptide's sequence. (genscript.com)
  • Peptide library: A group of large number of peptides typically ranging from tens to several millions or more with a systematic arrangement of amino acids in their sequence. (scientistsolutions.com)
  • A selected position in a peptide sequence is systematically replaced with different amino acid to show the effect on the substitute amino acid at certain position. (scientistsolutions.com)
  • A selected peptide sequence was found to inhibit proliferation of primary human vein endothelial cells, to have antiangiogenic effects in chick embryos, and finally to reduce breast tumor growth and metastasis by 70% in mice. (neb.com)
  • Tandem mass spectra contains specific information regarding the sequence of the peptide precursor, which can aid the identification of peptide/protein. (wikipedia.org)
  • In this approach, a protein sequence database is used to calculate all putative peptide candidates in the given setting (proteolytic enzymes, miscleavages, post-translational modifications). (wikipedia.org)
  • The sequence search engines use various heuristics to predict the fragmentation pattern of each peptide candidate. (wikipedia.org)
  • Sequence database searching also demands vast search space, which still could not cover all possibilities of peptide dynamics, exhibiting limited efficiency post-translational modifications). (wikipedia.org)
  • No purification given, as peptide purity is sequence-dependent and a function of different liquid chromatographic conditions. (thermofisher.com)
  • In the present report, we further examine the interaction of these two oxidase components by using random-sequence peptide phage display library analysis. (pnas.org)
  • The most distinguishing feature of this proposed lasso peptide is its uncommon core lasso peptide sequence. (princeton.edu)
  • After the sixth panning of the PDL, eight phage clones of 18 sequenced clones had the same sequence, LVDAAAL (named P1) and specific binding of P1 to the surface of iRBCs was confirmed using phage expressing P1 peptides and synthetic P1 peptide. (ajtmh.org)
  • and (3) imaging these bead-bound libraries to simultaneously identify the embedded spectral code (and thus the sequence of the associated peptide) and quantify the amount of each antibody bound. (spie.org)
  • Truncated peptide library can be used to identify the shortest amino acid sequence needed for a given function or activity. (genemedsyn.com)
  • This method was applied to VSV-G in order to construct a comprehensive library of mutants whose combined members have a His 6 tag inserted at likely every site in the original protein sequence. (asm.org)
  • The lack of sequence homology among the identified EF-Tu ligands demonstate promiscuous peptide binding by EF-Tu. (universityofcalifornia.edu)
  • 2006) PenBase, the shrimp antimicrobial peptide penaeidin database: sequence-based classification and recommended nomenclature. (oalib.com)
  • We demonstrated a method to screen for binders to a particular G-quadruplex sequence using easily designed short peptides consisting of naturally occurring amino acids and mining of binding data using statistical methods such as hierarchical clustering analysis (HCA). (acs.org)
  • Two 12-mer peptides containing this sequence, NV-O-R5-3 and NV-O-R5-6, were further characterized to evaluate the motif's functional ability to detect VLPs and virus. (ovid.com)
  • Studies using the purified phage and the corresponding synthetic cyclic peptides showed that *CWDDGWLC*-expressing phage binds specifically to fibronectin and to fibronectin fragments containing the RGD sequence. (rupress.org)
  • Coupling of peptide self-assembly to dynamic sequence exchange provides a useful approach for the discovery of self-assembling materials. (brad.ac.uk)
  • however, both its absolute and relative yield amplification is dictated by the properties of the donor component, indicating cooperativity of peptide sequence and donor/acceptor pairs in assembly. (brad.ac.uk)
  • Overlapping peptide libraries are generated by dividing the original protein or peptide sequence into many overlapping peptides of the same length. (jpt.com)
  • Scrambled libraries are constructed through permutation of the original peptide sequence. (neobiolab.com)
  • Clones selected from the libraries were sequenced and the peptides aligned with the original amino sequence of serine protease. (edu.au)
  • Positional Library - Positional scanning is an important tool for peptide sequence optimization. (abclonalbio.com)
  • Overlapping peptide libraries are also appropriate for scanning the primary sequence of proteins for linear, or "continuous", B-cell (antibody-defined) epitopes. (abclonalbio.com)
  • The last peptide included in the set represents the orphan sequence plus one or more N-Terminal residues to bring it to the correct length. (abclonalbio.com)
  • Cyclic peptide (middle) binds to iPGM (blue). (georgetown.edu)
  • The researchers identified the potential new therapeutic after testing more than a trillion small protein fragments, called cyclic peptides, to find one that could disable a vital enzyme in the disease-causing organisms, but leave similar enzymes in humans unscathed. (georgetown.edu)
  • Cyclic peptides have been a rich source of biologically active molecules. (osu.edu)
  • This method allows a single researcher to synthesize and screen up to ten million cyclic peptides and identify the most active ligand(s) in ~1 month, without the time-consuming and expensive hit resynthesis or the use of any special equipment. (osu.edu)
  • We also have another cyclic 9-mer peptide constructed using the same technical route. (labtube.tv)
  • Developing "hot-loop"-inspired cyclic peptides to inhibit the oncogenic Skp2-Cks1 protein-protein interaction. (tufts.edu)
  • This work involves using insight from a bioinformatics tool, LoopFinder, to design and test cyclic peptide inhibitors of a protein-protein interaction that is involved in cell cycle regulation and various types of cancer. (tufts.edu)
  • Over years, Creative Biolabs has established a comprehensive list of libraries for de novo discovery of therapeutically relevant antibodies and peptides. (creative-biolabs.com)
  • These well-tailored premade antibodies/peptides have made powerful toolbox to streamline our clients' therapeutic/diagnostic agent discovery pipeline. (creative-biolabs.com)
  • A large number of reputable references can be tracked, for whom we have raised excellent antibodies/peptides for research or commercial applications. (creative-biolabs.com)
  • The scaffold antibody gene one of the libraries, the M:anti-pep library, was chosen based on hypervariable loop canonical structures that are characteristic of other anti-peptide antibodies. (utexas.edu)
  • Additionally, all of the contact residues of this antibody are commonly used contact residues in other anti-peptide antibodies. (utexas.edu)
  • These positions and others were varied to incorporate the natural diversity of other anti-peptide antibodies. (utexas.edu)
  • Positions were chosen for variation based on their usage as contact residues in both anti-peptide and anti-protein antibodies. (utexas.edu)
  • The M:anti-pep library yielded specific antibodies to both peptides with dissociation constants as low as 14 nM against AT and 18 nM against NPY. (utexas.edu)
  • This peptide is immunogenic in mice, giving rise to strong antibody production, and most monoclonal antibodies made to breast cancer, which react with the protein core, react with the peptide APDTR. (begellhouse.com)
  • Polyclonal antibodies raised against the *CWDDGWLC*-peptide recognized beta 1 and beta 3 in immunoblots. (rupress.org)
  • Antibodies were used to screen a random peptide library of heptamers displayed on the surface of a bacteriophage. (microbiologyresearch.org)
  • To explore the effectiveness and reliability of random peptide libraries (Ph.D.™ and FliTrxTM libraries) for the selection of peptides, polyclonal antibodies against a recombinant serine protease from D. congolensis were used to pan the libraries. (edu.au)
  • To obtain peptides, which might be associated with a protective immune response to D. congolensis sheep which had been immunised with a crude enzyme preparation form D. congolensis by Dr T.M. Ellis were used as a source of antibodies. (edu.au)
  • There was a striking production of antibodies to antigens from D. congolensis induced by the peptides selected from the random peptide libraries. (edu.au)
  • Mass spectrometers are powerful tools that can fully identify and quantify very small amounts of peptides, which are fragments of the larger proteins. (eurekalert.org)
  • What's been missing, however, is a resource tool to check whether detected peptides originate from host cell proteins. (eurekalert.org)
  • Figure 4: Dependence of RXP chain length on protein stoichiometry of proteins involved in peptide elongation (Kj12B) and peptide termination (Kj12C) as shown by Western blot, HPLC/MS analysis and in vitro experiments using purified Kj12B and Kj12C. (nature.com)
  • PEPscreen peptide libraries have been used to study structural properties of proteins such as Human Thrombin. (sigmaaldrich.com)
  • One essential utility of a peptide spectral library is to serve as consensus templates supporting the identification of peptide/proteins based on the correlation between the templates with experimental spectra. (wikipedia.org)
  • citation needed] Spectral library searching is not applicable in a situation where the discovery of novel peptides or proteins is the goal. (wikipedia.org)
  • citation needed] However, the optimization for a particular module of the peptide spectra library is a more manageable goal, e.g. the proteins in a particular organelle or relevant to a particular biological phenotype. (wikipedia.org)
  • Thermo Scientific PEPotec Immuno Peptide Libraries are fully synthetic custom libraries that support high-throughput screening assays to map epitopes or identify immunogenic sites in proteins. (thermofisher.com)
  • Catalog Record: Amino acids, peptides, and proteins : an. (hathitrust.org)
  • The peptide length (5-20 aa) and offset number or the degree of overlapping amino acids (2-5 aa) is determined by the length of the full length proteins, overall experiment objectives and the cost. (genemedsyn.com)
  • This specificity determined for peptide substrates was also evident in many of the proteins phosphorylated by Dbf2-Mob1 in a proteome chip analysis. (caltech.edu)
  • Selections from phage-displayed combinatorial peptide libraries are an effective strategy for identifying peptide ligands to target proteins. (eurekaselect.com)
  • Pools of overlapping peptides from antigenic proteins for T-cell stimulation, T-cell expansion in T-cell assays such as ELISPOT, ICS or Flow Cytometry, e.g. for immune monitoring. (jpt.com)
  • We use this new compound to cyclize peptides on a phage-displayed library and screen the library against two model proteins. (tamu.edu)
  • Phage display is a laboratory technique for the study of protein-protein, protein-peptide, and protein-DNA interactions that uses bacteriophages (viruses that infect bacteria) to connect proteins with the genetic information that encodes them. (wikipedia.org)
  • In this way, large libraries of proteins can be screened and amplified in a process called in vitro selection, which is analogous to natural selection. (wikipedia.org)
  • We offer complimentary peptide library design services for overlapping peptide fragment libraries, alanine scanning libraries, truncation libraries and positional scanning libraries. (sigmaaldrich.com)
  • Overlapping Peptide Library - Overlapping peptide fragments to cover the full length of your protein of interest. (sigmaaldrich.com)
  • Peptide microarrays that display overlapping peptide scans through antigens from infectious organisms or tumor associated antigens for antibody or serum profiling. (jpt.com)
  • The overlapping peptides are defined by peptide length and peptide offset. (jpt.com)
  • This substantially enhances our existing siRNA programs by providing us with a huge proprietary library of human-derived targeting ligands to couple with our well-established and well-tolerated DPC and RONDEL delivery platforms. (nanowerk.com)
  • Additional binding assays showed that phage-displayed peptide ligands do not compete for binding to EF-Tu with various antibiotic EF-Tu inhibitors, and these ligands could identify an EF-Tu peptide binding site distinct from the antibiotic inhibitory sites. (universityofcalifornia.edu)
  • It may also be used to screen for new non-EF-Tu targets that bind to the ligands in this diverse library. (universityofcalifornia.edu)
  • Phoenix's Obesity Peptide Library has been used as screening new ligands for orphan receptor [Howard A. D., et al. (phoenixbiotech.net)
  • Phage display is a widely-used tool for the directed evolution of peptide ligands from large combinatorial peptide libraries. (tamu.edu)
  • We use the library containing this ncAA to select for peptide ligands of the lysine deacylase sirtuin 2. (tamu.edu)
  • To identify small peptides that would specifically recognize the altered surface of iRBCs, we screened a phage display peptide library (PDL) on the surface of iRBCs. (ajtmh.org)
  • The synthetic peptide library generated by Kuster and his colleagues allows researchers to evaluate this assumption and the techniques and algorithms the field has developed to employ it - a "benchmarking," he said, "that we felt was highly overdue. (genomeweb.com)
  • The binding of phage clones to Fny-CMV antiserum was inhibited by a synthetic peptide representing this region. (microbiologyresearch.org)
  • The invention relates to virus-like particles of bacteriophage MS2 (MS2 VLPs) displaying peptide epitopes or peptide mimics of epitopes of Nipah Virus envelope glycoprotein that elicit an immune response against Nipah Virus upon vaccination of humans or animals. (osti.gov)
  • The identification of suitable targets, coupled with the known immunogenicity of both the peptide and neo-carbohydrate epitopes, has led to the development of several different programs to immunize humans against breast cancer using either synthetic carbohydrates or peptides conjugated with adjuvants, and clinical trials are now in progress to evaluate their immunogenicity and anti-cancer effects. (begellhouse.com)
  • Antigen peptides represent specific epitopes for stimulation of T cells in T cell assays such as ELISPOT. (jpt.com)
  • Use of combinatorial peptide libraries to construct functional mimics of tumor epitopes recognized by MHC class I-restricted cytolytic T lymphocytes. (rupress.org)
  • We describe the use of combinatorial peptide libraries with defined amino acids in two MHC anchor positions to search for epitopes that are recognized by H-2Db- and Kb-restricted CTL specific for the mouse lymphoma EL4. (rupress.org)
  • Identification of epitopes in cucumber mosaic virus using a phage-displayed random peptide library. (microbiologyresearch.org)
  • While these libraries have been used to identify epitopes and prepare diagnostic tests, their potential to produce antigens which could generate a protective immune response has still to be explored. (edu.au)
  • In the course of this research, genomic and proteomic techniques have been used to investigate the bioactive peptides from the skin secretions of four American amphibian species: the Central American red-eyed leaf frog, Agalychnis callidryas~ the South American orange-legged leaf frog, Phyllomedusa hypochondrialis, Rohde's leaf frog, Phyllomedusa rohdei and the Giant Mexican leaf frog Pachymedusa dacnicolor. (bl.uk)
  • These discoveries of novel peptides from amphibian skin secretions have enriched our knowledge of bioactive peptides from this source and may provide the basis for several drug development programmes. (bl.uk)
  • Bioactive peptides, which can be used as cell-targeting or gene delivery agents, have been identified either by panning against purified receptors (19-24) or against intact cells or tissue samples, both in vitro and in vivo (25-33). (neb.com)
  • Once completed, the library is initially expected to comprise several hundred macrocycles that will be designed to potentially disrupt bioactive conformations commonly found in protein-protein interactions known to drive disease processes, and will deploy backbone scaffolds screened in silico for intrinsic cell permeability characteristics. (qb3.org)
  • Peptide libraries are a powerful tool in biological research for screening large numbers of peptides in the search for the few, critical bioactive peptides. (mimotopes.com)
  • Protease specificity profiling using proteome-derived, database-searchable peptide libraries is a novel approach to define the active site specificity of proteolytic enzymes we call PICS (Proteomic Identification of protease Cleavage Sites). (springer.com)
  • Proteome-derived peptide libraries are generated by trypsin, GluC, or chymotrypsin digestion of biologically relevant proteomes, such as cytosolic lysates, to generate three separate libraries that each differ from the others in their C-terminal amino acid residues according to the protease specificity. (springer.com)
  • Overall, the multi-stage activation approach produced more glycated peptide identifications, while the neutral-loss triggered MS3 approach resulted in much higher specificity. (unt.edu)
  • Jiang Z, Vasil AI, Gera L, Vasil ML, Hodges RS (2011) Rational design of alpha-helical antimicrobial peptides to target Gram-negative pathogens, Acinetobacter baumannii and Pseudomonas aeruginosa: utilization of charge, 'specificity determinants,' total hydrophobicity, hydrophobe type and location as design parameters to improve the therapeutic ratio. (oalib.com)
  • Truncation Library - Systematically remove flanking amino acids to determine the minimum length required for optimum activity. (sigmaaldrich.com)
  • With the knowledge of the positions of key residues elucidated through Alanine Scanning Library, the construction of the truncation library could also be centered around these key amino acid residues. (genscript.com)
  • Its state-of-the-art techniques, combined with clear step-by-step instructions, make this book an essential tool in the selection of peptides suitable for drug development. (springer.com)
  • Our results demonstrate that phage display technique is useful for selection of peptides that may represent valuable synthetic antigens for an improved serodiagnosis of CVL. (hindawi.com)
  • Selection of peptides targeting helix 31 of bacterial 16S ribosomal RNA by screening M13 phage-display libraries. (genefrontier.com)
  • Peptides were synthesized, polymerized with glutaraldehyde, and used as antigens in ELISA assays. (hindawi.com)
  • Once the existence of the lasso peptide was confirmed, purified crescenodin-1 underwent thermostability and carboxypeptidase Y assays to determine some of its characteristics. (princeton.edu)
  • Thermo Scientific PEPotec SRM Peptide Libraries provide fully synthetic, crude peptides customized for the development of mid- to high-throughput selected reaction monitoring (SRM) and multiple reaction monitoring (MRM) assays in quantitative mass spectrometry (MS) workflows. (thermofisher.com)
  • In this regard, peptide libraries can serve as biological assays: by adding cells or microbes to an array of peptides, researchers can easily trace a variety of biological outcomes from cell differentiation to cytotoxicity. (genscript.com)
  • Peptide sets and pools, as well as assay standardization kits are available with stable isotope labeled or unlabeled proteotypic peptides for mass-spectrometry based proteomics such as MRM assays. (jpt.com)
  • Bicyclic peptide inhibitor reveals large contact interface with a protease target," ACS Chemical Biology , vol. 7, no. 5, pp. 817-821, 2012. (hindawi.com)
  • Primary amines of all peptides are then chemically protected so that after incubation with a test protease, the neo-N-termini of the prime-side cleavage products with exposed α-amines can be specifically biotinylated, enriched, and identified by liquid chromatography-tandem mass spectrometry. (springer.com)
  • These application-specific peptide libraries can be used in applications such as vaccine development, T- and B-cell research, antibody development, biomarker discovery, and kinase and protease studies. (thermofisher.com)
  • Vaccination with recombinant serine protease and with the peptides selected from the Ph.D.™ library increased the rate of resolution of the lesions caused by one strain of D. congolensis. (edu.au)
  • Mimotopes' proprietary process transforms unpurified "crude" peptide libraries, into fully-purified peptides that have all been prepared to minimum 70%, 80%, 90% or 95% purity by reverse-phase HPLC, regardless of initial "crude" purity. (mimotopes.com)
  • In this work we varied in a combinatorial fashion the ring sizes of bicyclic peptides in phage libraries, expecting that they would yield binders with higher affinities and/or more diverse binding motifs that could be affinity matured. (rsc.org)
  • Some of these peptides may bind in orientations that allow affinity maturation of non-conserved regions, while others do not. (rsc.org)
  • Having available multiple leads isolated from such bicyclic peptide libraries with variable ring sizes could therefore be a great asset for the generation of high affinity binders. (rsc.org)
  • In such systems, inorganic binding peptides may be an essential component due to their high affinity and selective binding features onto different types of solid surfaces. (cambridge.org)
  • The structures of the peptides exhibiting high affinity were characterised by Edman microsequencing. (diva-portal.org)
  • While the implementation of the technology provided here is a high-affinity antibody/protein interaction with a small code space, we believe this platform can be broadly applicable to any range of peptide screening applications, with the capability to multiplex into libraries of hundreds to thousands of peptides in a single assay. (spie.org)
  • Selecting the library via iterative retroviral infections of mammalian cells led to the identification of several VSV-G-His 6 variants that were able to package high-titer viral vectors and could be purified by Ni-nitrilotriacetic acid affinity chromatography. (asm.org)
  • Phage-displayed peptides with high affinity for EF-Tu were selected from the library. (universityofcalifornia.edu)
  • Moreover, fibronectin bound to a *CWDDGWLC*-peptide affinity column, and could be eluted with an RGD-containing peptide. (rupress.org)
  • The specific interaction between biotin and avidin was exploited in the affinity purification of solid-phase synthesized peptide libraries. (cnrs-orleans.fr)
  • This ultrasound-based method enables single-step selection of a high-affinity peptide. (wikipedia.org)
  • Alanine, the smallest chiral natural amino acid is used to substitute non-alanine residues at each position of the original peptide. (genscript.com)
  • The Alanine Scanning Library can be used to determine the relative importance of each substituted residues toward the original peptide's functionality since knocked off key residues reflect diminished peptide activity. (genscript.com)
  • Achieved by substituting selected amino acid residues by all other natural amino acids one at a time, it has the capability to locate potential more favorable residue(s) at specified position(s) for enhanced peptide activity. (genscript.com)
  • Say you wanted a peptide chain 10 residues in length to use in native chemical ligation with a larger recombinantly expressed protein. (wikipedia.org)
  • It is created by generating a set of peptides with systematic truncation of the flanking residues. (scientistsolutions.com)
  • Results: Using a combination of peptide library selection, phosphorylation of opitmal peptide variants, and screening of a phosphosite array, we found that Dbf2-Mob1 preferentially phosphorylated serine over threonine and required an arginine three residues upstream of the phosphorylated serine in its substrate. (caltech.edu)
  • Synthetic peptides corresponding to this region in beta 3 were found to bind RGD-displaying phage and conversion of its two aspartic residues into alanines greatly reduced the RGD binding. (rupress.org)
  • The library is constructed by systemically removing the flanking residues of the original peptide. (abclonalbio.com)
  • Alanine Scanning Library - Alanine Scanning is an approach to identify specific amino acid residues responsible for a peptide's activity. (abclonalbio.com)
  • These application-specific peptide libraries are ready to use in applications such as vaccine development, T and B cell research, antibody development, and biomarker discovery. (thermofisher.com)
  • As a complement to our peptide library services, NovoPro provides 6 free peptide library design tools to support research projects from structural biology to vaccine development. (novoprolabs.com)
  • Random peptide libraries provide an alternative approach to the identification of peptides which might be useful in a vaccine. (edu.au)
  • The xdiscover™ library is a proprietary collection of more than 2000 primarily venom-derived peptides and is the result of a unique genomics capability that allows for the highly efficient discovery of novel peptides from cone snail venom tissue. (biospace.com)
  • Here, we present a method comprising binding studies of serum antibody pools to synthetic random peptide libraries, and data analysis of the resulting binding patterns. (nih.gov)
  • With over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. (creative-biolabs.com)
  • Of note, we also provide immunized human antibody libraries generated from diseased patients. (creative-biolabs.com)
  • In addition to phage display, high-quality antibody libraries (scFv/Fab/sdAb/full-length IgG/Fn3) for yeast display are available as well. (creative-biolabs.com)
  • a fluorogenic biosensor was developed by conjugating a fluorophore with a designated Cys at the antigen-binding site of antibody library on the phage [ 4 ]. (mdpi.com)
  • In this report, we present a new technology for measuring antibody-peptide interactions in vitro that leverages spectrally encoded beads for biological multiplexing. (spie.org)
  • This dissertation describes several strategies used to create diversity in non-immune antibody libraries. (utexas.edu)
  • Both of these strategies used to create these antigen-class focused libraries used a single scaffold antibody gene that contained diversity only in the variable heavy region. (utexas.edu)
  • The second library, the Hu:anti-pep, is based on a widely used, unique combination of human germline antibody segments that express well in bacterial expression. (utexas.edu)
  • Extremely high rates of mutagenesis (2.2% of the gene to 2.7%) were used to create two libraries of the anti-digoxin antibody 26-10. (utexas.edu)
  • The libraries had been screened by others in an attempt to examine the effects of highrates of mutagenesis on the directed evolution of an antibody. (utexas.edu)
  • This study confirmed that high-error rate antibody libraries contain more active clones than expected. (utexas.edu)
  • Combinations of the selected consensus mutations from these libraries provide moderate enhancements to the kinetics and expression of the wild-type antibody in a non-synergistic manner. (utexas.edu)
  • It is now also clear that humans with breast cancer have, in their draining lymph nodes, precursors of cytotoxic T cells that can be stimulated in vitro to react against breast cancer and indeed against the APDTR or a closely related peptide − shown from antibody-blocking studies. (begellhouse.com)
  • Representing the human proteome by tandem mass spectra of synthetic peptides alleviates some of the current issues with protein identification and quantification. (technologynetworks.com)
  • A patent by George Pieczenik claiming priority from 1985 also describes the generation of peptide libraries. (wikipedia.org)
  • Shmuel Cabilly presents in Combinatorial Peptide Library Protocols a collection of new and unique techniques for the construction and use of peptide libraries. (springer.com)
  • Combinatorial Peptide Library Protocols offers novice and experienced investigators alike the ability to select molecules from a randomized pool having specific biological activities. (springer.com)
  • Can be and uphold combinatorial peptide library protocols 1998 points of this murder to illuminate samples with them. (mrwhitsettinc.com)
  • combinatorial peptide library protocols hit half the Forty-five, u, or name into Robin Longstride that he ended into Maximus. (mrwhitsettinc.com)
  • geographically, but the combinatorial peptide library protocols you need blocking for makes temporarily located shown. (mrwhitsettinc.com)
  • NeoPeptide utilizes proprietary technologies for the design and manufacture of large combinatorial arrays of peptides , peptidometics and peptide derivatives. (neobiolab.com)
  • All purified peptides are delivered with complete QC data including RP-HPLC, MS, and COA report to ensure high quality. (genemedsyn.com)
  • The concept of combinatorial peptide libraries originated at Mimotopes (Geysen, M., 1984) and in 2009, Mimotopes launched the peptide industry's first fully-HPLC purified peptide library platform. (mimotopes.com)
  • Stringent quality control: NovoPro provides Certificate of Analysis, MS, and HPLC validation data for each peptide. (novoprolabs.com)
  • MS and HPLC validation data for each peptide. (neobiolab.com)
  • Because of the commercial availability of large random peptide libraries displayed on phage and flagellin there is an opportunity to produce low cost and immunologically potent peptide vaccines. (edu.au)
  • In 1990, Jamie Scott and George Smith described creation of large random peptide libraries displayed on filamentous phage. (wikipedia.org)
  • Peptide length in a given library is usually 5-20 aa with 1-5 amino acid overlap. (genemedsyn.com)
  • Chemical diversity of the biologically active RXPs results from a combination of iterative and flexible use of monomodular nonribosomal peptide synthetases including substrate promiscuity, enzyme cross-talk and enzyme stoichiometry as shown by in vivo and in vitro experiments. (nature.com)
  • A selected position on the lead peptide is replaced with different amino acid (natural or unnatural) to improve the peptide properties such as biological activity, enzyme stability and solubility. (sigmaaldrich.com)
  • These signatures are called mass spectra, which characterize individual peptides. (eurekalert.org)
  • We have all gone by the assumption that the experimental mass spectra will resemble that of a theoretical [spectra] we put together by calculating the b and y ions of the typical peptide fragmentation. (genomeweb.com)
  • citation needed] One potential application of peptide spectral libraries is the identification of new, currently unknown mass spectra. (wikipedia.org)
  • Spectral libraries have been used in the small molecules mass spectra identification since the 1980s. (wikipedia.org)
  • the mass spectra corresponding to the fragments of peptide precursor is recorded. (wikipedia.org)
  • Such derivative patterns are used as templates to find a sufficiently close match within experimental mass spectra, which serves as the basis for peptide/protein identification. (wikipedia.org)
  • Due to the complex nature of peptide fragmentation in a mass spectrometer, derivative fragmentation patterns fall short of reproducing experimental mass spectra, especially relative intensities among distinct fragments. (wikipedia.org)
  • Fortunately, more and more high-quality mass spectra are being acquired by the collective contribution of the scientific community, which will continuously expand the coverage of peptide spectral library. (wikipedia.org)
  • The researchers also used the library to compare collision induced dissociation and electron transfer dissociation, determining that CID allowed for the identification of more peptides and phosphopeptides. (genomeweb.com)
  • Method to compare collision-induced dissociation spectra of peptides: Potential for library searching and subtractvie analysis. (wikipedia.org)
  • While electron transfer dissociation (ETD) has been shown to outperform collision-induced dissociation (CID) in sequencing glycated peptides by tandem mass spectrometry, ETD instrumentation is not yet available in all laboratories. (unt.edu)
  • Empowered by extensive experience and well-established phage display technique platform, Creative Biolabs now brings out a comprehensive list of premade peptide libraries for various research programs. (labtube.tv)
  • Natural product-like macrocyclic N-methyl-peptide inhibitors against a ubiquitin ligase uncovered from a ribosome-expressed de novo library," Chemistry & Biology , vol. 18, no. 12, pp. 1562-1570, 2011. (hindawi.com)
  • Brogden KA (2005) Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria? (oalib.com)
  • We demonstrate that peptides identified from this selection, when synthesized with a structural analog of NƐ-butyryl-lysine, NƐ-thiobutyryl-lysine, are potent inhibitors of sirtuin 2-catalyzed lysine deacylation. (tamu.edu)
  • To facilitate access to the library, the researchers have struck an arrangement with Thermo Fisher Scientific under which they will provide the company with the library and the company will distribute it to interested scientists for what Kuster said will be a small handling fee. (genomeweb.com)
  • The consortium of TUM, JPT Peptide Technologies (JPT), SAP and Thermo Fisher Scientific has made the vast quantity of data freely available to the scientific community via the data analytics platform ProteomicsDB (www.proteomicsdb.org) and the data repository PRIDE (www.ebi.ac.uk/pride) to enable scientists and to foster collaboration around the globe. (technologynetworks.com)
  • Thermo Fisher Scientific has introduced the Thermo Scientific PEPotec Immuno Custom Peptide Library Service. (technologynetworks.com)
  • JPT has been designing and synthesizing peptide libraries for peptide microarrays , peptide pools or as peptide libraries of individual peptides for many years, and we are happy to provide recommendations to choose the most efficient peptide library design for your specific application. (jpt.com)
  • The truncation process is carried out via a systemic reduction of resides from each flank of the original peptide. (genscript.com)
  • It's constructed by substituting selected positions on the original peptide randomly and simultaneously with all other natural amino acids in a shot gun approach with a purpose to elucidate potential alternatives for enhanced peptide activity. (genscript.com)
  • To request a quote or help designing a custom peptide library, please complete the online form . (sigmaaldrich.com)
  • The standard PEPotec Immuno Custom Peptide Library provides lyophilized mass spectrometry-tested peptides in a 96-well tube array format. (technologynetworks.com)
  • To learn more about the standard PEPotec Immuno Custom Peptide Library Service and optional services, please visit www.thermoscientific.com/pepotec-immuno . (technologynetworks.com)
  • Blocking protein-protein interaction with peptides discovered by phage display will have broad clinical applications for treatment of metastasizing tumors. (neb.com)
  • The present report concerns the generation of specific markers and the establishment of a selection procedure for microglia specific molecules from phage displayed peptide libraries. (semanticscholar.org)
  • Most of our competitors use the spot technology providing poor quality peptides above 15 amino acids. (thermofisher.com)
  • Purified, fully analyzed, high quality peptides, synthesized from the peptide experts for Alzheimers and prion research. (jpt.com)
  • At MDACC, Drs. Pasqualini and Arap used a technique called phage display to generate a vast library of specific peptides that home to both known and novel receptors. (nanowerk.com)
  • MHC Multimers for reproducible detection, enumeration and isolation of antigen-specific T cells with disease specific peptides. (jpt.com)
  • 1 edition of Neurotensin, a brain and gastrointestinal peptide found in the catalog. (openlibrary.org)
  • As validated by QC DNA sequencing, over 97% of randomly picked clones from these two libraries contained dsDNA inserts, with no stop codon, single nucleotide deletion or insertion or non-sense frame-shift mutation detected. (labtube.tv)
  • The Hu:anti-pep library yielded specific clones with higher dissociation constants: 49 nM against NPY and 11 [mu]M against AT. (utexas.edu)
  • A total of 91 isolated clones from both libraries were sequenced. (utexas.edu)
  • Such "TAG-obligate" libraries overcome obstacles associated with libraries that contain both TAG and non-TAG clones. (tamu.edu)
  • The library is provided in a convenient 96-well plate format adapted for screening purposes. (anaspec.com)
  • The second type of peptide libraries are called random libraries which generates a mixture (pool) of several thousand or millions of peptides in each reaction chamber thereby facilitating rapid screening of a large number of peptides. (scientistsolutions.com)
  • Not only does this discovery raise hope for better treatments for many parasitic and bacterial diseases, it highlights the value of screening peptides in the search for ways to treat conditions that do not respond well-or have stopped responding-to more traditional chemical drug compounds. (georgetown.edu)
  • Screening p47-phox with the peptide libraries identified five potential sites of interaction with flavocytochrome b, including three previously reported regions of interaction and two additional regions of interaction of p47-phox with gp91-phox and p22-phox. (pnas.org)
  • This work shows that combined computational and experimental screening can be used complementarily and in combination providing a powerful means to discover new supramolecular peptide nanostructures. (rsc.org)
  • The acquisition also enables Arrowhead to further expand the library by working with Drs. Pasqualini and Arap to generate additional data with more patient screening at MDACC. (nanowerk.com)
  • Peptides can be made in required amounts from minimal to mg scale for initial screening. (genemedsyn.com)
  • Going beyond RGD: screening of a cell-adhesion peptide library in 3D cell culture. (physiciansweekly.com)
  • SOUTH SAN FRANCISCO, Calif.--(BUSINESS WIRE)--Circle Pharma, Inc., today announced that it will apply its computational design and synthetic chemistry platform to design and create a physical screening library of novel macrocyclic peptides. (qb3.org)
  • Conclusion: We have determined by peptide library selection and phosphosite array screening that the protein kinase Dbf2-Mob1 preferentially phosphorylated substrates that contain an RXXS motif. (caltech.edu)
  • these fusions can be evaluated for repressor activity using direct selection with λ phage, or a variety of reporter genes suitable for library screening. (springer.com)
  • This study illustrates how a peptide library can be designed and presents a screening guideline for construction of G-quadruplex binders. (acs.org)
  • Michael D. Scholle, John W. Kehoe and Brian K. Kay, " Efficient Construction of a Large Collection of Phage-Displayed Combinatorial Peptide Libraries", Combinatorial Chemistry & High Throughput Screening (2005) 8: 545. (eurekaselect.com)
  • 2. A tool for finding new leads by creating a random screening library. (neobiolab.com)
  • Modifications to the extracellular domain of VSV-G, however, have been hindered by a lack of structural knowledge of the protein and limited identification of permissible insertion sites ( 14 , 28 , 37 ), making it an excellent candidate for applying a novel library mutagenesis method to improve its functionality. (asm.org)
  • Existing protocols for constructing phage-displayed libraries utilize either ligation into double-stranded phage DNA or Kunkel mutagenesis with single-stranded phagemid DNA. (eurekaselect.com)
  • The Ph.D.™-C7C Phage Display Peptide Library Kit contains a tube of the Ph.D.-C7C Phage Library, protein reagents for a control reaction and sequencing primer stocks. (neb.com)
  • Construction of such a peptide-fused phage library possessing non-natural core structures will be useful for future drug discovery. (mdpi.com)