Aptamers, Peptide: Peptide sequences, generated by iterative rounds of SELEX APTAMER TECHNIQUE, that bind to a target molecule specifically and with high affinity.Aptamers, Nucleotide: Nucleotide sequences, generated by iterative rounds of SELEX APTAMER TECHNIQUE, that bind to a target molecule specifically and with high affinity.SELEX Aptamer Technique: A method of generating a large library of randomized nucleotides and selecting NUCLEOTIDE APTAMERS by iterative rounds of in vitro selection. A modified procedure substitutes AMINO ACIDS in place of NUCLEOTIDES to make PEPTIDE APTAMERS.Titanium: A dark-gray, metallic element of widespread distribution but occurring in small amounts; atomic number, 22; atomic weight, 47.90; symbol, Ti; specific gravity, 4.5; used for fixation of fractures. (Dorland, 28th ed)Corrosion: The gradual destruction of a metal or alloy due to oxidation or action of a chemical agent. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Bone Substitutes: Synthetic or natural materials for the replacement of bones or bone tissue. They include hard tissue replacement polymers, natural coral, hydroxyapatite, beta-tricalcium phosphate, and various other biomaterials. The bone substitutes as inert materials can be incorporated into surrounding tissue or gradually replaced by original tissue.Materials Testing: The testing of materials and devices, especially those used for PROSTHESES AND IMPLANTS; SUTURES; TISSUE ADHESIVES; etc., for hardness, strength, durability, safety, efficacy, and biocompatibility.Coated Materials, Biocompatible: Biocompatible materials usually used in dental and bone implants that enhance biologic fixation, thereby increasing the bond strength between the coated material and bone, and minimize possible biological effects that may result from the implant itself.Antigens, Human Platelet: Human alloantigens expressed only on platelets, specifically on platelet membrane glycoproteins. These platelet-specific antigens are immunogenic and can result in pathological reactions to transfusion therapy.Thrombocytopenia, Neonatal Alloimmune: A condition in newborns caused by immunity of the mother to PLATELET ALLOANTIGENS on the fetal platelets. The PLATELETS, coated with maternal ANTIBODIES, are destroyed and removed by the fetal MONONUCLEAR PHAGOCYTE SYSTEM. Affected infants may have INTRACRANIAL HEMORRHAGES.Antibodies, Immobilized: Antibodies that are chemically bound to a substrate material which renders their location fixed.Blood Platelets: Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.Blood Coagulation: The process of the interaction of BLOOD COAGULATION FACTORS that results in an insoluble FIBRIN clot.Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.Neurons, Afferent: Neurons which conduct NERVE IMPULSES to the CENTRAL NERVOUS SYSTEM.Ganglia, Spinal: Sensory ganglia located on the dorsal spinal roots within the vertebral column. The spinal ganglion cells are pseudounipolar. The single primary branch bifurcates sending a peripheral process to carry sensory information from the periphery and a central branch which relays that information to the spinal cord or brain.TRPV Cation Channels: A subgroup of TRP cation channels named after vanilloid receptor. They are very sensitive to TEMPERATURE and hot spicy food and CAPSAICIN. They have the TRP domain and ANKYRIN repeats. Selectivity for CALCIUM over SODIUM ranges from 3 to 100 fold.MicroRNAs: Small double-stranded, non-protein coding RNAs, 21-25 nucleotides in length generated from single-stranded microRNA gene transcripts by the same RIBONUCLEASE III, Dicer, that produces small interfering RNAs (RNA, SMALL INTERFERING). They become part of the RNA-INDUCED SILENCING COMPLEX and repress the translation (TRANSLATION, GENETIC) of target RNA by binding to homologous 3'UTR region as an imperfect match. The small temporal RNAs (stRNAs), let-7 and lin-4, from C. elegans, are the first 2 miRNAs discovered, and are from a class of miRNAs involved in developmental timing.Tissue Scaffolds: Cell growth support structures composed of BIOCOMPATIBLE MATERIALS. They are specially designed solid support matrices for cell attachment in TISSUE ENGINEERING and GUIDED TISSUE REGENERATION uses.RNA: A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Nucleic Acid Conformation: The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.RNA, Untranslated: RNA which does not code for protein but has some enzymatic, structural or regulatory function. Although ribosomal RNA (RNA, RIBOSOMAL) and transfer RNA (RNA, TRANSFER) are also untranslated RNAs they are not included in this scope.Colorimetry: Any technique by which an unknown color is evaluated in terms of standard colors. The technique may be visual, photoelectric, or indirect by means of spectrophotometry. It is used in chemistry and physics. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Biosensing Techniques: Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal.Uranium Compounds: Inorganic compounds that contain uranium as an integral part of the molecule.Remote Sensing Technology: Observation and acquisition of physical data from a distance by viewing and making measurements from a distance or receiving transmitted data from observations made at distant location.Laboratories, Dental: Facilities for the performance of services related to dental treatment but not done directly in the patient's mouth.Dental Technicians: Individuals responsible for fabrication of dental appliances.Technology, Dental: The field of dentistry involved in procedures for designing and constructing dental appliances. It includes also the application of any technology to the field of dentistry.Biology: One of the BIOLOGICAL SCIENCE DISCIPLINES concerned with the origin, structure, development, growth, function, genetics, and reproduction of animals, plants, and microorganisms.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Stereoisomerism: The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.Inventions: A novel composition, device, or process, independently conceived de novo or derived from a pre-existing model.Xeroderma Pigmentosum Group D Protein: A DNA helicase that is a component of TRANSCRIPTION FACTOR TFIIH. It plays an essential role in NUCLEOTIDE EXCISION REPAIR, and mutations in this protein are associated with XERODERMA PIGMENTOSUM.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.DNA Glycosylases: A family of DNA repair enzymes that recognize damaged nucleotide bases and remove them by hydrolyzing the N-glycosidic bond that attaches them to the sugar backbone of the DNA molecule. The process called BASE EXCISION REPAIR can be completed by a DNA-(APURINIC OR APYRIMIDINIC SITE) LYASE which excises the remaining RIBOSE sugar from the DNA.Marketing: Activity involved in transfer of goods from producer to consumer or in the exchange of services.Newspapers: Publications printed and distributed daily, weekly, or at some other regular and usually short interval, containing news, articles of opinion (as editorials and letters), features, advertising, and announcements of current interest. (Webster's 3d ed)Journalism, Medical: The collection, writing, and editing of current interest material on topics related to biomedicine for presentation through the mass media, including newspapers, magazines, radio, or television, usually for a public audience such as health care consumers.Research Report: Detailed account or statement or formal record of data resulting from empirical inquiry.Food: Any substances taken in by the body that provide nourishment.Hunger: The desire for FOOD generated by a sensation arising from the lack of food in the STOMACH.Food, Genetically Modified: Food derived from genetically modified organisms (ORGANISMS, GENETICALLY MODIFIED).Beak: In some animals, the jaws together with their horny covering. The beak usually refers to the bill of birds in which the whole varies greatly in form according of the food and habits of the bird. While the beak refers most commonly to birds, the anatomical counterpart is found also in the turtle, squid, and octopus. (From Webster, 3d ed & Storer, et al., General Zoology, 6th ed, p491, 755)Strikes, Employee: Work-related situations in which the employees as a group refuse to work until certain conditions of employment are granted by the employer.Food Supply: The production and movement of food items from point of origin to use or consumption.Organisms, Genetically Modified: Organisms whose GENOME has been changed by a GENETIC ENGINEERING technique.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Smad4 Protein: A signal transducing adaptor protein and tumor suppressor protein. It forms a complex with activated RECEPTOR-REGULATED SMAD PROTEINS. The complex then translocates to the CELL NUCLEUS and regulates GENETIC TRANSCRIPTION of target GENES.Smad3 Protein: A receptor-regulated smad protein that undergoes PHOSPHORYLATION by ACTIVIN RECEPTORS, TYPE I. Activated Smad3 can bind directly to DNA, and it regulates TRANSFORMING GROWTH FACTOR BETA and ACTIVIN signaling.
Known examples of smart ligands include DNA smart aptamers; however, RNA and peptide smart aptamers can also be developed. ... For example, a panel of DNA smart aptamers has been recently used to develop affinity analysis of proteins with ultra-wide ... Baaske P, Wienken CJ, Reineck P, Duhr S, Braun D (Feb 2010). "Optical Thermophoresis quantifies Buffer dependence of Aptamer ... Drabovich AP, Berezovski M, Okhonin V, Krylov SN (May 2006). "Selection of smart aptamers by methods of kinetic capillary ...
Furthermore, the company offers Aptamers, small DNA, RNA (or peptide) molecules that show the ability to bind to specific ... "United States Patent - Streptavidin-binding peptide and uses thereof". "United States Patent - Peptides with sequentially ... Aptamers have been widely used in medical and industrial fields. 1996 - Institut für Bioanalytik GmbH was founded by Herbert ... Ho, D.; Falter, K.; Severin, P.; Gaub, H. E. (2009). "DNA as a force sensor in an aptamer-based biochip for adenosine". Anal ...
"Design and validation of a neutral protein scaffold for the presentation of peptide aptamers". J. Mol. Biol. 352 (5): 1118-33. ... Stabilisation of the two peptides by the protein scaffold constrains the possible conformations that the peptides can take, ... Affimer proteins display two peptide loops and an N-terminal sequence that can all be randomised to bind to desired target ... "Exploration of the HIF-1α/p300 interface using peptide and adhiron phage display technologies". Mol. Biosyst. 11 (10): 2738-49 ...
... as in the case of aptamers, or from non-immunoglobulin protein scaffolds / peptide aptamers, into which hypervariable loops are ... 2005). "P. Design and validation of a neutral protein scaffold for the presentation of peptide aptamers". J Mol Biol. 352: 1118 ... Within the protein scaffold there exist two variable peptide loops and a variable N-terminal sequence that provide a high ... CS1 maint: Multiple names: authors list (link) Ladner, R.C. (1995). "Constrained peptides as binding entities". Trends Biotech ...
DNA aptamers, which are peptides that have with specific target molecules that they search for, fold in response to the ... Electrochemical Aptamer-Based Sensor". Journal of the American Chemical Society. 131 (12): 4262-4266. doi:10.1021/ja806531z. ... Aptamer-Based Tracking of Circulating Therapeutic Agents in Living Animals". Science Translational Medicine. 5 (213): 213ra165- ... this field was initiated with monitoring of small-molecule cocaine levels in undiluted blood serum with electrochemical aptamer ...
... es are often conceptually divided into two parts: an aptamer and an expression platform. The aptamer directly binds ... Two classes of glutamine riboswitches are known: the glnA RNA motif and Downstream-peptide motif. These classes are believed to ... as it contains contiguous dual aptamers. Though no longer shown to be cooperative, the cause of dual aptamers still remains ... of the relevant genes and the success of procedures to create artificial small molecule-binding RNAs called aptamers. In 2002, ...
Peptide nucleic acid
... aptamers and ribozymes. In 2015 Jain et. al. described a trans-acting DNA-based amphiphatic delivery system for convenient ... Peptide synthesis Oligonucleotide synthesis Glycol nucleic acid Threose nucleic acid Clicked peptide polymer Nielsen PE, Egholm ... Peptide nucleic acid (PNA) is an artificially synthesized polymer similar to DNA or RNA. It was invented by Peter E. Nielsen ( ... June 2006). "Peptide Nucleic Acid (PNA): A Review". Journal of Chemical Technology and Biotechnology. An overview of the PNA ...
Ames TD, Breaker RR (January 2011). "Bacterial aptamers that selectively bind glutamine". RNA Biol. 8 (1). doi:10.4161/rna.8.1. ... Downstream-peptide RNAs are found upstream of short open reading frames (ORFs) that are predicted to encode short peptides ( ... and their structural similarity to Downstream-peptide RNAs in turn suggests that Downstream-peptide RNAs are also riboswitches ... Downstream-peptide RNAs overlap a predicted non-coding RNA called yfr6 that is over 200 nucleotides in length, but it was ...
Among peptides obtained from biopannings, mimotopes can be considered as a kind of peptide aptamers. All the peptides panned ... Peptide aptamers. They consist of one (or more) short variable peptide domains, attached at both ends to a protein scaffold. ... Peptide aptamers can also be selected from combinatorial peptide libraries constructed by phage display and other surface ... The Affimer protein, an evolution of peptide aptamers, is a small, highly stable protein engineered to display peptide loops ...
DNA aptamers, which are peptides that have with specific target molecules that they search for, fold in response to the ... "Real-Time, Aptamer-Based Tracking of Circulating Therapeutic Agents in Living Animals". Science Translational Medicine. 5 (213 ... this field was initiated with monitoring of small-molecule cocaine levels in undiluted blood serum with electrochemical aptamer ... "Continuous, Real-Time Monitoring of Cocaine in Undiluted Blood Serum via a Microfluidic, Electrochemical Aptamer-Based Sensor" ...
L-Ribonucleic acid aptamer
Like other aptamers, L-RNA aptamers are able to bind molecules such as peptides, proteins, and substances of low molecular ... such as PEGylated L-RNA aptamers, show a prolonged plasma half-life. Unlike other aptamers, L-RNA aptamers are not directly ... L-RNA aptamers are a form of aptamers. Due to their L-nucleotides, they are highly resistant to degradation by nucleases. ... In contrast to other aptamers, L-RNA aptamers have high stability in blood serum, since they are less susceptible to be cleaved ...
They are usually artificial peptides or proteins with a molar mass of about 3 to 20 kDa. Nucleic acids and small molecules are ... Affimer Antibody mimetic Anti-mitochondrial antibodies Anti-nuclear antibodies Aptamer Colostrum ELISA Humoral immunity ... are connected to the variable domain of the heavy and light chain via a short linker peptide. The linker is rich in glycine, ...
Reference peptides are printed on the slides to allow for protein quantification of the sample lysates. RPAs allow for the ... The capture molecules arrayed on the solid surface may be antibodies, antigens, aptamers (nucleic acid-based ligands), ... In this technique, a library of antibodies, aptamers or affibodies is arrayed on the support surface. These are used as capture ... Shin, DS; Kim, DH; Chung, WJ; Lee, YS (30 September 2005). "Combinatorial solid phase peptide synthesis and bioassays". Journal ...
Threose nucleic acid
GlnA RNA motif
Nucleic acid analogue
In 2013, they applied the Ds-Px pair to DNA aptamer generation by in vitro selection (SELEX) and demonstrated the genetic ... Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol ... Feb 2005). "Crystal structure of a partly self-complementary peptide nucleic acid (PNA) oligomer showing a duplex-triplex ... 2013). "Generation of high-affinity DNA aptamers using an expanded genetic alphabet". Nat. Biotechnol. 31 (5): 453-457. doi: ...
The method involves fluorescently labeling peptide molecules that would alter an organism's natural pathway. When this peptide ... Likewise, the Spinach aptamer is an engineered RNA sequence which can bind GFP chromophore chemical mimics, thereby conferring ... Because of the exact defined change that these isotopes incur on the peptides, it is possible to tell through the spectrometry ... The most commonly labelled molecules are antibodies, proteins, amino acids and peptides which are then used as specific probes ...
DNA-encoded chemical library
According to this split-and-pool protocol a peptide combinatorial library DNA-encoded of 106 members was generated. In 2001 ... mRNA display and aptamer SELEX. In antibody phage display, antibodies are physically linked to phage particles that bear the ... peptide encoding, haloaromatic tagging, encoding by secondary amines, semiconductor devices.), mainly to avoid inconvenient ... "Generation and screening of an oligonucleotide-encoded synthetic peptide library". Proc. Natl. Acad. Sci. U.S.A. 90 (22): 10700 ...
Magnetic resonance imaging
Commonly, peptides, antibodies, or small ligands, and small protein domains, such as HER-2 affibodies, have been applied to ... Liu CH; Ren J; Liu CM; Liu PK (2014). "Intracellular gene transcription factor protein-guided MRI by DNA aptamers in vivo". ... "Measurement of the degree of isotopic enrichment of different positions in an antibiotic peptide by NMR" (PDF). Journal of ...
Heat shock protein
Walsh NP, Alba BM, Bose B, Gross CA, Sauer RT (April 2003). "OMP peptide signals initiate the envelope-stress response by ... Salamanca HH, Fuda N, Shi H, Lis JT (August 2011). "An RNA aptamer perturbs heat shock transcription factor activity in ... Borges JC, Ramos CH (April 2005). "Protein folding assisted by chaperones". Protein and Peptide Letters. 12 (3): 257-61. doi: ... Moreover, HSF1 inhibition by a potent RNA aptamer attenuates mitogenic (MAPK) signaling and induces cancer cell apoptosis. ...
... peptides, DNA, nucleic acid aptamers, or small-molecule ligands  can be used to target quantum dots to specific ... Peptides are being researched as potential quantum dot material. Since peptides occur naturally in all organisms, such dots ... Hauser, Charlotte A. E.; Zhang, Shuguang (2010). "Peptides as biological semiconductors". Nature. 468 (7323): 516-517. Bibcode: ... Whaley SR, English DS, Hu EL, Barbara PF, Belcher AM (2000). "Selection of peptides with semiconductor binding specificity for ...
Brain-derived neurotrophic factor
... is a pegylated anti-vascular endothelial growth factor (VEGF) aptamer, a single strand of nucleic acid that binds ... Pituitary adenylate cyclase-activating peptide (PACAP). *Pleiotrophin. *Renalase. *Thrombopoietin (see here instead) ... After the administration of the aptamer into rhesus monkeys, no toxic effects where exhibited. It was also noted that there was ...
Insulin-like growth factor 2
Epidermal growth factor
Allows construction of Cas9 complexes with protein binding cassettes, artificial aptamers, pools of random sequences as well as ... and inability to distinguish lncRNA function from other confounding factors like cryptically encoded peptides or functional DNA ... artificial aptamers and small molecules with varying size. While all the complexes were functional and viable, and successfully ...
Keratinocyte growth factor
Regulation of gene expression
... (NTFs) are a family of biomolecules - nearly all of which are peptides or small proteins - that support ... Aptamers: Against NGF: RBM-004. *Decoy receptors: LEVI-04 (p75NTR-Fc) ... pituitary adenylate cyclase-activating peptide (PACAP), interleukin-1 (IL-1), interleukin-2 (IL-2), interleukin-3 (IL-3), ...
While DNA is the dominant material used, structures incorporating other nucleic acids such as RNA and peptide nucleic acid (PNA ... and aptamers, which can bind to specific proteins or small molecules. Structural DNA nanotechnology, sometimes abbreviated as ... Emerging applications of DNAzymes and aptamers". Current Opinion in Biotechnology. 17 (6): 580-588. doi:10.1016/j.copbio. ...
Xeno nucleic acid
PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds and regulate protein folding and maturation. ... "Comparing human pancreatic cell secretomes by in vitro aptamer selection identifies cyclophilin B as a candidate pancreatic ... "Selective assay for CyPA and CyPB in human blood using highly specific anti-peptide antibodies". J. Immunol. Methods. 178 (1): ... this protein catalyzes the cis-trans isomerization of proline imidic peptide bonds, which allows it to regulate protein folding ...
Peptide Aptamers-Endothelial Cells (P36), Peptide Aptamer, FITC labelled-Genemed Synthesis Inc.
FITC labelled Peptide Aptamers AP-308-F Endothelial Cells (P36), Peptide Aptamer, FITC labelled Peptide Aptamers AP-308-F ... Endothelial Cells (P36), Peptide Aptamer, FITC labelled. Peptide Aptamers. 5 mg. Contact Sales ... Peptide Aptamer, FITC labelled (Peptide Aptamers) A-Z Product Search ... Endothelial Cells (P36), Peptide Aptamer, FITC labelled. Catalog#. Product Description. Product Type. Antibody Type. Size. ...
Targeting LMO2 with a peptide aptamer establishes a necessary function in overt T-cell neoplasia. - Radcliffe Department of...
We have addressed this using a peptide aptamer that binds to the second LIM domain of the LMO2 protein and disrupts its ... The peptide inhibits Lmo2 function in a mouse T-cell tumor transplantation assay by preventing Lmo2-dependent T-cell neoplasia ... We have addressed this using a peptide aptamer that binds to the second LIM domain of the LMO2 protein and disrupts its ... Targeting LMO2 with a peptide aptamer establishes a necessary function in overt T-cell neoplasia. ...
Ultra-high sensitivity of the non-immunological affinity of graphene oxide-peptide-based surface plasmon resonance biosensors...
Specific peptide aptamers can be used in place of expensive antibody proteins, and they are gaining increasing importance as ... N2 - Specific peptide aptamers can be used in place of expensive antibody proteins, and they are gaining increasing importance ... AB - Specific peptide aptamers can be used in place of expensive antibody proteins, and they are gaining increasing importance ... abstract = "Specific peptide aptamers can be used in place of expensive antibody proteins, and they are gaining increasing ...
Peptide aptamer identified by molecular docking targeting translationally controlled tumor protein in leukemia cells |...
Bioinformatics screening and molecular docking analyses were utilized to select high affinity peptides targeting ... translationally controlled tumor protein (TCTP). Selected peptide aptamers were tested... ... Selected peptide aptamers were tested towards cancer cell lines with different levels of TCTP expression. One peptide (WGQWPYHC ... Cancer Fortilin Histamine releasing factor Leukemia Molecular docking Peptide aptamer TCTP This is a preview of subscription ...
Therapeutic peptide delivery via aptamer-displaying, disulfide-linked peptide amphiphile micelles - Molecular Systems Design &...
While previous research has shown aptamer-displaying PAMs enhance cell association, transportation to intracellular targets ... are a powerful platform technology for improving the delivery of therapeutic and prophylactic peptides. ... Therapeutic peptide delivery via aptamer-displaying, disulfide-linked peptide amphiphile micelles J. D. Smith, L. N. Cardwell, ... Peptide amphiphile micelles (PAMs) are a powerful platform technology for improving the delivery of therapeutic and ...
Nucleic Acid and Peptide Aptamers | Springer for Research & Development
Divided conveniently into two distinct parts beginning with nucleic acid aptamers and ending with peptide aptamers, the volume ... Isolation of Peptide Aptamers to Target Protein Function Luisa Lopez-Ochoa, Tara E. Nash, Jorge Ramirez-Prado, Linda Hanley- ... Peptide Aptamers for Small Molecule Drug Discovery Carine Bardou, Christophe Borie, Marc Bickle, Brian B. Rudkin, Pierre Colas ... In Nucleic Acid and Peptide Aptamers: Methods and Protocols, expert researchers contribute state-of-the-art methods focused on ...
Functionalization of biomedical surfaces by peptide aptamers - Open Research Online
Functionalization of biomedical surfaces by peptide aptamers.. In: 27th European Conference on Biomaterials, 30 Aug - 3 Sep ... to increase the interaction of nano hydroxyapatite and titanium oxide using an organic interphase of peptides known as aptamers ... proteins and peptides. Some studies suggested that a second bioactive material should be deposited, sprayed or attracted to the ...
Inhibition of mammalian cell proliferation by genetically selected peptide aptamers that functionally antagonize E2F activity
... ... Here, we use a two hybrid approach to isolate from combinatorial libraries peptide aptamers that specifically interact with ... Our experiments argue that the variable region of this aptamer is structured, and that it functions by binding E2F with a motif ... These results show that cell proliferation can be inhibited using genetically-selected synthetic peptides that specifically ...
Nano-hydroxyapatite deposition on titanium using peptide aptamers - Open Research Online
Nano-hydroxyapatite deposition on titanium using peptide aptamers.. In: 14th Annual Conference of the UK Society for ... to increase the interaction of nano hydroxyapatite and titanium oxide using an organic interphase of peptides known as aptamers ... peptide chemistry; surface science; hydroxyapatite; titanium; surface engineering; surface chemistry; orthopaedics; surface ... proteins and peptides. Some studies suggested that a second bioactive material should be deposited, sprayed or attracted to the ...
Frontiers | Genetic Selection of Peptide Aptamers That Interact and Inhibit Both Small Protein B and Alternative Ribosome...
The peptide aptamer PA-2 was selected from pTRG-SN-peptides by bacterial two-hybrid system (B2H) employing pBT-SmpB or pBT-ArfA ... The peptide aptamer PA-2 was selected from pTRG-SN-peptides by bacterial two-hybrid system (B2H) employing pBT-SmpB or pBT-ArfA ... For identification of specific peptides that interact and inhibit the stalled-ribosome rescues, peptide aptamer library (pTRG- ... peptide aptamer library (pTRG-SN-peptides) was constructed using pTRG as vector and Staphylococcus aureus nuclease (SN) as ...
A novel assay for the detection of anti-human platelet antigen antibodies (HPA-1a) based on peptide aptamer technology |...
23 a peptide aptamer able to specifically bind this particular antibody should mimic the HPA-1a antigen. Such a peptide aptamer ... A peptide aptamer library was screened using an anti-HPA-1a human monoclonal antibody as a bait to isolate an aptamer that ... Screening of the peptide aptamer library. To set up a new detection assay for antiHPA-1a antibodies, we screened a peptide ... A peptide aptamer specific for human anti-HPA-1a antibodies. We describe the isolation and characterization of a peptide ...
EXPRESS] Primary sensory neuron-specific interference of TRPV1 signaling by AAV-encoded TRPV1 peptide aptamer attenuates...
Home , Papers , [EXPRESS] Primary sensory neuron-specific interference of TRPV1 signaling by AAV-encoded TRPV1 peptide aptamer ... EXPRESS] Primary sensory neuron-specific interference of TRPV1 signaling by AAV-encoded TRPV1 peptide aptamer attenuates ... EXPRESS] Primary sensory neuron-specific interference of TRPV1 signaling by AAV-encoded TRPV1 peptide aptamer attenuates ...
Peptides-&-Proteins - Aptamer Group
Methods and compositions for F-18 labeling of proteins, peptides and other molecules - Immunomedics, Inc.
Aptamer Labeling. An aptamer comprising an aldehyde is conjugated to the Al-F-18 NOTA thiosemicarbazide of Example 21. The F-18 ... Al was added to the peptide and its HPLC profile compared to the empty NOTA peptide and the Al-F-18 peptide. The Al peptide and ... Peptide Synthesis. The peptides were synthesized by solid phase peptide synthesis using the Fmoc strategy. Groups were added to ... labeled peptide), two hours (77% labeled peptide) and four hours (76% labeled peptide) of incubation in human serum (not shown ...
Aptamers :: ATW0026 - Latency Associated Peptide (LAP) Aptamer
ATW0026 Aptamer to Human Latency Associated Peptide TGF-beta is secreted by many cell types including macrophages in a latent ... form in which it is complexed with two other polypeptides latent TGF-beta binding protein LTBP and latency associated peptide ... ATW0026 - Latency Associated Peptide (LAP) Aptamer. Amount* (µg):. Please, select one. 25 100 ... ATW0026 Aptamer to Human Latency Associated Peptide TGF-beta is secreted by many cell types, including macrophages, in a latent ...
RNA Scaffold & Aptamer by KUN LV on Prezi
2.How to design? Aptamers are oligonucleic acid or peptide molecules that bind to a specific target molecule. Aptamers are ... Aptamer Aptamer More details Details How to Select? Classification Features What is Aptamer? What is RNA Scaffold? Things left ... Researches on aptamers have achieved much progress in biosensor, drug development and nano technology. Normally 25-60 nt. ... High affinity and specificity Aptamers are selected by SELEX(systematic evolution of ligands by exponential enrichment) in ...
Evaluation of human epidermal growth factor receptor 2 in breast cancer with a novel specific aptamer | Cellular & Molecular...
The selected aptamer was an 86-nucleotide DNA molecule that bound to an epitope peptide of HER2 with a Kd of 18.9 nM. The ... Figure 1. Evaluation of HER2 in breast cancer with the aptamer HB5. (a) Binding profiles of the aptamer HB5 to HER2-positive ( ... we propose a novel IHC method using a newly developed DNA aptamer (HB5). The aptamer HB5 was shown to be capable of ... Novel HER2 aptamer selectively delivers cytotoxic drug to HER2-positive breast cancer cells in vitro. J Transl Med 2012; 10: ...
Aptamer-based colorimetric sensor systems - Lu, Yi
The present invention provides an aptamer-based calorimetric sensor system for determining the presence and optionally the ... The aptamer 124 may be formed from deoxyribonucleotides, which may be natural, unnatural, or modified nucleic acids. Peptide ... After selecting an appropriate aptamer or aptamers in 120, a linker 128 is formed that includes the aptamer 124. In one aspect ... The aptamer selection 120 may provide one or more aptamers that demonstrate enhanced folding in the presence of the selected ...
Global Aptamers Market Technology Trend Analysis Market Report 2013-2018: Therapeutics, Diagnostics, Biosensors, Biomarker/Drug...
Aptamers Market - Technology Trend Analysis By Applications - Therapeu...,Global,Aptamers,Market,Technology,Trend,Analysis, ... By Aptamer Type. - Nucleic acid aptamers. - Peptide aptamers. Key Topics Covered:. 1 Introduction. 2 Executive Summary. 3 ... the basics on nucleic acid aptamers, peptide aptamers and the SELEX method for their synthesis are also discussed. Research ... Aptamer Sciences Inc. - Aptamer Solutions Ltd. - Aptares AG. - Aptusbiotech. - Base Pair Biotechnologies. - Bioapter SL. - Cd ...
Pharmaceuticals | Free Full-Text | Aptamers for Targeted Drug Delivery | HTML
Therefore, aptamers that bind cell surface receptors have been exploited for the delivery of a variety of cargoes into cells. ... Therefore, relatively few aptamers have been generated that bind cell surface receptors. However, improvements in recombinant ... Several studies have demonstrated that aptamers that bind cell surface receptors may have functions other than just blocking ... This review focuses on recent progress and current challenges in the field of aptamer-mediated delivery. ...
PEPTIDE APTAMERS FOR MANIPULATING PROTEIN FUNCTION - Patent application
2. The peptide aptamer expression cassette of claim 1, further comprising a promoter. 3. The peptide aptamer expression ... 1. A peptide aptamer expression cassette comprising a DNA sequence encoding a polyprotein that constrains a peptide aptamer by ... 0005] Peptide aptamers are short peptides of random amino acid sequences. As commonly used, these peptides are generally 15-20 ... Peptide aptamers and the methods to produce cassettes including the aptamers and manipulating them, are described. The peptide ...
Aptamer Market to reach US$5.0 bn by 2025 - TMR
Global aptamer market was valued at nearly US$1.0 bn in 2016 and is expected to expand with a CAGR of 20.0% over the forecast ... Aptamer Market Segmentation by Material Type - Nucleic acid Aptamer, Peptide Aptamer; Selection Technique Type - SELEX ... Based on material type the global aptamer market is segmented into nucleic acid aptamer and peptide aptamer. The material type ... Aptamer Market (Material - Nucleic acid Aptamer, Peptide Aptamer; Selection Technique - SELEX Technique and Others; Application ...
Tumor-targeted silencing of the peptide transporter TAP induces potent antitumor immunity | Nature Communications
Treatment with the chemically-synthesized nucleolin aptamer-TAP siRNA conjugate represents a broadly-applicable approach to ... Administration of TAP siRNA conjugated to a broad-range tumor-targeting nucleolin aptamer inhibited tumor growth in multiple ... and induced the presentation of a TAP-independent peptide in human tumor cells. ... Aptamer-targeted siRNA downregulation of TAP in tumor cells. We first confirmed that the AS1411 nucleolin aptamer used in our ...
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... - published on openPR.com ... Aptamers are oligonucleotides or peptide molecules that bind to target lipid, protein, or nucleic acid molecules with a high ... Aptamers Market Latest Innovation in 2019 by SOMALOGIC, APTAMER GROUP, APTAMER S … ... Aptamers are oligonucleotides or peptide molecules that bind to target lipid, protein, or nucleic acid molecules with a high ...
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Peptide aptamers - artificial short peptides with specific binding affinity for target molecules - can be used to interfere ... Therefore, peptide aptamers have emerged as a new, powerful tool with high efficiency and specificity and wide applications in ... Peptide aptamers can also be applied to produce broad-spectrum viral resistance in plants. For example, based on the ... The authors further demonstrated that peptide aptamers can be extended to produce virus resistance in tomato (Solanum ...
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Peptide aptamers - artificial short peptides with specific binding affinity for target molecules - can be used to interfere ... Therefore, peptide aptamers have emerged as a new, powerful tool with high efficiency and specificity and wide applications in ... Peptide aptamers can also be applied to produce broad-spectrum viral resistance in plants. For example, based on the ... The authors further demonstrated that peptide aptamers can be extended to produce virus resistance in tomato (Solanum ...
IJMS | Free Full-Text | Aptamers and Their Potential to Selectively Target Aspects of EGF, Wnt/β-Catenin and TGFβ-Smad Family...
By their targeting of short, linear motif type of interactions, peptide aptamers have joined nucleic acid aptamers for use in ... We propose that peptide aptamers can provide a very useful and new alternative for interfering with protein-protein ... Here, we present a brief survey of the use of aptamers in signaling pathways, in particular of polypeptide growth factors, ... We then discuss the opportunities for using aptamers in other complex pathways, including Wnt/β-catenin, and focus on ...
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The use of peptide aptamers selected in vivo to recognize specific protein isoforms, the ability to functionalize each ... We describe a method to immobilize specific peptide aptamers on individual electrodes at the micrometer scale, together with a ... We present a novel strategy that offers practical solutions to these challenges, featuring peptide aptamers as artificial ... protein kinases in whole-cell lysates using arrays of ten electrodes functionalized with individual peptide aptamers, with no ...
ProteinsProteinKineticSensitivityFunctionSELEXMoleculesSpecificityProteins and peptidesLigandsAffinityTumorShare of the global aptamer marketApplications of aptamersAssayAssaysMonoclonalTransparency Market ResearchField of aptamer technologySynthesisDetectionVitroNeuropathic painShort peptidesMolecularRNAsDiagnosticsAptagenDrug ConjugatesLigandConjugatesBindsTherapeuticsMethodsCustom aptamerDevelop new aptamers2018AS1411PharmaceuticalCombinatorial librariesSpecificNovel aptamersNatural AptamersThrombinAdvantagesCharacterizationChemicallyMacugenSequenceMicroarrays
- Specific peptide aptamers can be used in place of expensive antibody proteins, and they are gaining increasing importance as sensing probes due to their potential in the development of non-immunological assays with high sensitivity, affinity and specificity for human chorionic gonadotropin (hCG) protein. (ntnu.edu.tw)
- We have addressed this using a peptide aptamer that binds to the second LIM domain of the LMO2 protein and disrupts its function. (ox.ac.uk)
- In addition to the improved affinity offered by the high compatibility with the target hCG protein, the major advantage of GO-peptide-based SPR sensors was their reduced nonspecific adsorption and enhanced sensitivity. (ntnu.edu.tw)
- The sensitivity of the slope ratio of the low concentration hCG protein assay in linear regression analysis was GO-peptide (1 mM): GO-peptide (0.1 mM): conventional chip (8-mercaptooctanoic acid)-peptide (0.1 mM)=8.6: 3.3: 1. (ntnu.edu.tw)
- The GO-peptide-based chip (1 mM) had a high affinity (K A ) of 6.37×10 12 M −1 , limit of detection of 0.065 nM and ultra-high sensitivity of 16 times that of a conventional SPR chip. (ntnu.edu.tw)
- In summary, the excellent binding affinity, low detection limit, high sensitivity, good stability and specificity suggest the potential of this GO-peptide-based SPR chip detection method in clinical application. (ntnu.edu.tw)
- High affinity and specificity Aptamers are selected by SELEX(systematic evolution of ligands by exponential enrichment) in vitro. (prezi.com)
- A novel HER2 aptamer (HB5) has recently been developed using systematic evolution of ligands by exponential enrichment technology (SELEX). (nature.com)
- SELEX products were named 'aptamers,' which are single-stranded RNA or DNA oligonucleotides that bind with high affinity to specific targets such as proteins, small molecules, nucleic acids and cells. (nature.com)
- As well as a discussion on the properties of aptamers in the technological analysis, the basics on nucleic acid aptamers, peptide aptamers and the SELEX method for their synthesis are also discussed. (bio-medicine.org)
- On the basis of the selection technique, the market is segmented into the SELEX technique and other techniques (X-aptamer and MARAS technique). (transparencymarketresearch.com)
- Among these, SELEX technique is expected to account for the leading share in the global market for aptamer over the forecast period from 2017 to 2025. (transparencymarketresearch.com)
- This growth is attributable to the advancement of the techniques such as CE-SELEX and M-SELEX which helps to overcome some of the limitations in the other procedures for selection of aptamers. (transparencymarketresearch.com)
- The procedure commonly used for the selection of aptamers for a specific target molecule is known as SELEX process. (transparencymarketresearch.com)
- SELEX technique is likely to get boost for the modification of existing SELEX process, and available of the new updated SELEX technique like CE-SELEX, M- SELEX etc. which overcome the certain limitation for aptamer selection. (openpr.com)
- Such aptamers can be isolated from combinatorial libraries by SELEX (systematic evolution of ligands by exponential enrichment) [ 3 ]. (mdpi.com)
- Generated by Systematic Evolution of Ligands by Exponential Enrichment (SELEX), aptamers are short synthetic oligonucleotides, either DNA or RNA, that specifically bind target molecule(s) with high specificity due to their specific and complex three-dimensional shape comprised stems, loops, bulges, hairpins, pseudoknots, triplexes, and quadraplexes [ 12 - 14 ]. (hindawi.com)
- Nucleic acid aptamers are nucleic acid species that have been engineered through repeated rounds of in vitro selection or equivalently, SELEX (systematic evolution of ligands by exponential enrichment) to bind to various molecular targets such as small molecules, proteins, nucleic acids, and even cells, tissues and organisms. (wikipedia.org)
- Using SELEX (systematic evolution of ligands by exponential enrichment), we serendipitously discovered a ssDNA aptamer that binds selectively to the anti-FLAG M2 antibody. (hindawi.com)
- These findings demonstrate the feasibility of using SELEX to develop ssDNA aptamers that block the function of a specific antibody, a capability that could lead to the development of novel therapeutic modalities for patients with systemic lupus erythematosus, rheumatoid arthritis, and other autoimmune diseases. (hindawi.com)
- Normally aptamers can be generated by the aptamer selection process known as SELEX process. (mynewsdesk.com)
- Several modifications of the SELEX procedure have been introduced to improve aptamer selectivity or to reduce selection time. (mynewsdesk.com)
- Subtractive SELEX, automated SELEX and atomic force microscopy-SELEX are some of the modified SELEX processes available for aptamer selection with desired features. (mynewsdesk.com)
- Generally, aptamers can be artificially generated in vitro by a process commonly referred to as SELEX (Systematic Evolution of Ligands by Exponential Enrichment) [ 18 ], which consists of several repeated rounds of binding, partition, and amplification [ 2 ]. (biomedcentral.com)
- In SELEX experiments, aptamers are identified for their abilities to bind a protein of interest from libraries containing up to 10 16 different RNA or DNA sequences [ 19 ]. (biomedcentral.com)
- Because SELEX typically takes several rounds to isolate target-binding aptamers, you will start with a known aptamer mixture rather than than a completely random library. (mit.edu)
- The immense diversity in function and structure of nucleic acids enable numerous aptamers to be generated through an iterative in vitro selection technique known as Systematic Evolution of Ligands by EXponential enrichment (SELEX). (scirp.org)
- More complex aptamer identification methods, using whole cancer cells (Cell-SELEX), may identify novel targets and aptamers to affect them. (scirp.org)
- Nucleic acid aptamers are engineered through SELEX or in vitro selection. (antibodies-online.com)
- Aptamer are selected for specific target molecules by an in vitro selection and amplification method called SELEX. (ufz.de)
- These molecules were found to self-assemble in water into PAMs for which lipidated DNA oligomers ( i.e., antitail amphiphiles - AAs) could be entrapped and used to tether aptamers (Apt) to the nanoparticle surface. (rsc.org)
- In particular embodiments, the labeled molecules may be peptides or proteins, although other types of molecules including but not limited to aptamers, oligonucleotides and nucleic acids may be labeled and utilized for such imaging studies. (freepatentsonline.com)
- Thus, there are typically five to fifteen times as many moles or molecules of aptamers present in a solution containing an equal mass. (basepairbio.com)
- Aptamers are oligonucleic acid or peptide molecules that bind to a specific target molecule. (prezi.com)
- Though there are just approximately 60 companies (biotech and pharmaceutical) working on aptamers, there are quite a number of research groups focused on the diverse applications of aptamers, particularly in studying key target molecules involved in cardiovascular diseases, infectious diseases, and against viral antigens. (bio-medicine.org)
- Binding sites for aptamers include clefts and grooves of target molecules (including enzymes) resulting in antagonistic activity very similar to many currently available pharmaceutical agents. (mdpi.com)
- Aptamers are special class of molecules that combine the advantages of both low molecular weight and protein molecules. (transparencymarketresearch.com)
- Aptamers illustrate lock-and-key relationship between aptamer molecules and binding partners i.e., target molecules. (transparencymarketresearch.com)
- They called these RNA sequences-and later similarly behaving and selected single-stranded DNA molecules [ 2 ]-aptamers. (mdpi.com)
- Aptamers are oligonucleotides that bind to target lipid, protein, or nucleic acid molecules with a high specificity. (emailwire.com)
- Aptamers are able to bind a wide variety of targets from divalent metal ions, small organic molecules, proteins, and cells [ 15 , 16 ]. (hindawi.com)
- Aptamers (from the Latin aptus - fit, and Greek meros - part) are oligonucleotide or peptide molecules that bind to a specific target molecule. (wikipedia.org)
- Extension of aptamer selection from natural components to from unnatural components realizes the creation of new functional molecules. (rsc.org)
- offering customers access to an accelerated aptamer selection platform that delivers tailor-made high-affinity, highly specific molecules for use in a variety of scenarios. (onenucleus.com)
- Aptamers, first reported by Ellington and Gold in 1990 [ 1 , 2 ], are single stranded DNA/RNA molecules or peptide molecules [ 3 ]. (biomedcentral.com)
- The immobilization of a vast number of substances and molecules, ranging from inorganic calcium phosphate phases up to peptides and proteins, has been investigated throughout recent decades. (royalsocietypublishing.org)
- The methods can be used for electrical detection of molecular interactions between probe molecules bound to defined regions of an array and protein or peptide target molecules which are permitted to interact with the probe molecules. (google.com)
- 70: 3419-25, describe an apparatus and method for detecting interactions between immobilized fiuorescently-labeled aptamers and peptide target molecules. (google.com)
- Aptamers are DNA, RNA or peptide molecules that bind to specific molecular targets. (thehindubusinessline.com)
- Aptamers are nucleic acid or peptide based binders that specifically recognized proteins, nucleic acids, and other molecules. (antibodies-online.com)
- Aptamers are single-stranded DNA or RNA molecules that can bind to pre-selected targets including proteins and peptides with high affinity and specificity. (cambio.co.uk)
- Aptamer affinity chromatography has been used to purify small molecules, proteins, and even for cell separation. (cambio.co.uk)
- Aptamers can also distinguish between highly conserved molecules. (cambio.co.uk)
- Debora MacKenzie , New Scientist , 6 Oct. 2006 Aptamers … are isolated by … the repetitive screening of large pools of randomized molecules for those that bind best to a target. (merriam-webster.com)
- 17. A method according to claim 1 wherein said immobilized probe molecules are peptides. (google.co.uk)
- Peptide aptamers are recombinant proteins which can interact with any given protein target with high specificity. (haematologica.org)
- In contrast to antisense oligonucleotides and small interfering RNAs (siRNAs) that inhibit translation of proteins by Watson-Crick base-pairing to their respective messenger RNAs, aptamers bind to existing proteins (and, less commonly, non-protein targets) with high affinity and specificity, analogous to monoclonal antibodies. (mdpi.com)
- The nucleolin aptamer used in our study as prototype of tumor-targeting ligand, exhibits specificity to a broad range of tumor cells of both murine and human origin, and was used to target biological agents to tumor cells in mice 17 . (nature.com)
- By their targeting of short, linear motif type of interactions, peptide aptamers have joined nucleic acid aptamers for use in signaling studies because of their ease of production, their stability, their high specificity and affinity for individual target proteins, and their use in high-throughput screening protocols. (mdpi.com)
- Aptamers can be engineered to have higher specificity and affinity for number of targets. (emailwire.com)
- The binding specificity and affinity of aptamers have long been harnessed as the key elements in the development of aptamer-based assays, particularly aptasensing application. (hindawi.com)
- One promising avenue that is currently explored based on the specificity and affinity of aptamers is the application of aptamers in the decontamination assays. (hindawi.com)
- Binding specificity and affinity of aptamers are harnessed in various applications, majorly in biosensing applications [ 10 , 11 , 17 - 26 ]. (hindawi.com)
- Short fragments of RNA - called RNA aptamers - can have secondary structures that also allow them to bind a target molecule with good affinity and specificity. (mit.edu)
- Nanoparticles that are suitable for detection and loading with drugs can be directed to the site of their action by use of the affinity and specificity of aptamers, which are conjugated to the nanoparticles or fixed on their surface. (smw.ch)
- Aptamers can facilitate cell-specific drug delivery in a selective manner to the sick cancer cells because of their binding specificity. (smw.ch)
- X-aptamers incorporate chemically modified nucleotides and DNA backbones to achieve a greater range of possible targets or increase affinity and specificity. (antibodies-online.com)
- Aptamers with the desired specificity are selected using the yeast two hybrid system or established display techniques. (antibodies-online.com)
- Peptides are designed with consideration of selectivity of radiolabel incorporation and specificity for the intended target. (nih.gov)
Proteins and peptides2
- The titanium oxide layer plays an important role on the surface of Ti allowing deposition of biomaterials, cells, proteins and peptides. (open.ac.uk)
- A major problem in drug delivery is the effective absorption of high molecular weight material such as proteins and peptides across biological membranes. (google.es)
- Nucleic acid aptamers are short, single-stranded RNAs and DNAs that represent high-affinity and highly selective ligands for different targets, ranging from large proteins to peptides, nucleotides, drugs and small compounds, and even metal ions. (mdpi.com)
- The Szostak lab also coined the term aptamer (from the Latin, apto, meaning 'to fit') for these nucleic acid-based ligands. (wikipedia.org)
- Two years later, the Szostak lab and Gilead Sciences, independent of one another, used in vitro selection schemes to evolve single stranded DNA ligands for organic dyes and human coagulant, thrombin (see Anti-thrombin aptamers), respectively. (wikipedia.org)
- While the process of artificial engineering of nucleic acid ligands is highly interesting to biology and biotechnology, the notion of aptamers in the natural world had yet to be uncovered until 2002 when two groups led by Ronald Breaker and Evgeny Nudler discovered a nucleic acid-based genetic regulatory element (which was named riboswitch) that possesses similar molecular recognition properties to the artificially made aptamers. (wikipedia.org)
- Lately, a concept of smart aptamers, and smart ligands in general, has been introduced. (wikipedia.org)
- Our goal was to generate oligonucleotide ligands that recognize tumor-associated proteins on/within living cells, simultaneously identifying target proteins and DNA aptamers. (pnas.org)
- First, most aptamers bind to targets with high affinity, demonstrating typical dissociation constants in the pico- to nanomolar range. (mdpi.com)
- Because of their target affinity, aptamers can direct the transport of aptamer-nanoparticle conjugates. (smw.ch)
- Aptamers offer several unique characteristics that are desirable for affinity chromatography. (cambio.co.uk)
- Aptamers can be selected under pH, ionic strength, and temperature conditions favourable for affinity chromatography. (cambio.co.uk)
- The use of aptamer affinity columns to remove environmental contaminants has also been demonstrated. (cambio.co.uk)
- Modifications to produce aptamers with slower off-rates combined with new support materials are making aptamers even better choices for affinity chromatography. (cambio.co.uk)
- RNA aptamers are nanomolecules that can recognize their targets with extremely high affinity. (aacrjournals.org)
- Aptamers are receiving increasing attention as alternative affinity reagents and represent essential tools in both basic and applied research. (ufz.de)
- One peptide (WGQWPYHC) revealed specific cytotoxicity according to the TCTP expression in tumor cells without affecting normal cells. (springer.com)
- Administration of TAP siRNA conjugated to a broad-range tumor-targeting nucleolin aptamer inhibited tumor growth in multiple tumor models without measurable toxicity, was comparatively effective to vaccination against prototypic mutation-generated neoantigens, potentiated the antitumor effect of PD-1 antibody or Flt3 ligand, and induced the presentation of a TAP-independent peptide in human tumor cells. (nature.com)
- Treatment with the chemically-synthesized nucleolin aptamer-TAP siRNA conjugate represents a broadly-applicable approach to increase the antigenicity of tumor lesions and thereby enhance the effectiveness of immune potentiating therapies. (nature.com)
- With the aim of developing a clinically translatable and broadly applicable approach to induce TAP deficiency-induced neoantigens in tumor cells in vivo, we used siRNA to downregulate TAP that was targeted to murine and human tumor cells by conjugation to a broad-range nucleolin-binding oligonucleotide aptamer. (nature.com)
- The peptide inhibits Lmo2 function in a mouse T-cell tumor transplantation assay by preventing Lmo2-dependent T-cell neoplasia. (ox.ac.uk)
- One possible approach is the use of aptamers to deliver siRNAs selectively to tumor cells with appropriate antigens displayed on the surface. (eurekaselect.com)
- We have probed antigens presented by a monolayer of tumor cells for their ability to interact with a pool of aptamers. (pnas.org)
- In tumor-bearing mice, this anti-IL4Rα aptamer preferentially targeted MDSCs and TAM and unexpectedly promoted their elimination, an effect that was associated with an increased number of tumor-infiltrating T cells and a reduction in tumor growth. (aacrjournals.org)
Share of the global aptamer market2
- However, the diagnostics segment accounted for the 30% share of the global aptamer market in 2016. (transparencymarketresearch.com)
- As key players introduce their new aptamer services for different application in developed markets such a North America and Western Europe, these regions are likely to account for dominating share of the global aptamer market. (openpr.com)
Applications of aptamers1
- Demands for new diagnostic assay and increasing research activity in emerging markets such as China and Japan is likely to boost growth of Asia Pacific aptamer market at a CAGR of around 21.0% from 2017 to 2025. (openpr.com)
- Analogous to the point-of-care diagnostics, there is no doubt that aptamers can also be deployed in the point-of-care aptamer-based decontamination assay, whereby decontamination can be performed anywhere and anytime for instantaneous decision-making. (hindawi.com)
- Apart from biosensing application, aptamers have also been the subject in the aptamer-based capture assay. (hindawi.com)
- The present review seeks to present an overview of the more narrowed context of the aptamer-based capture assay, which is on the usage of the aptamer in the decontaminating process or simply abbreviated as apta-decontamination assay. (hindawi.com)
- Several aspects of the apta-decontamination assay will be deliberated, which encompasses the functionalization of the aptamer to the usage of the aptamer in decontaminating environment, food sample, and in in vivo application. (hindawi.com)
- Reusability of the aptamer and the development of point-of-care apta-decontamination assays are explored before envisaging the potential future trend of the apta-decontamination assay. (hindawi.com)
- An aptamer-based dot blot assay is more robust and can be designed for point-of-care clinical testing and on-site environmental and industrial analysis. (cambio.co.uk)
- While antibody-based immunoassays have proven to be very sensitive and specific, there are some limitations which can be overcome with the ELASA, or Enzyme-Linked Aptamer Sorbent Assay. (cambio.co.uk)
- Research applications are specifically discussed regarding aptamers use in flow cytometry, reagents and assays, mass spectrometry and western blotting. (bio-medicine.org)
- 11. A method to generate a phenotype, the method comprising using the peptide expression cassette of claim 1 and using target independent assays to detect the phenotype. (patentsencyclopedia.com)
- Specifically, the colour change caused by their aggregation or disaggregation and connected to the aptamer-target binding reaction has been used in the development of assays and tests. (smw.ch)
- They are particular suitable for quantum dot-stimulated FRET-based (beacon) aptamer assays . (smw.ch)
Transparency Market Research1
Field of aptamer technology1
- Peptide Aptamers-Endothelial Cells (P36), Peptide Aptamer, FITC labelled-Genemed Synthesis Inc. (genemedsyn.com)
- Microarrays designed for RNA aptamer screening and binding optimization and built on the flexible and powerful Paraflo microfluidic on-chip synthesis platform. (bio-medicine.org)
- So, the Middle-East and African market for aptamers is expected to grow, which has been backed by advancements in technology with respect to selection processes and synthesis. (emailwire.com)
- s r.o. offers the synthesis of peptides with the chain length from 2 to 40 amino acid moieties in demanded purity. (cebiotech.com)
- For the peptide synthesis the recent technologies in the art are used, like automatic peptide synthesizer, microwave device and modern HPLC systems. (cebiotech.com)
- Other features of aptamers like favorable toxicological property, high stability, ease of synthesis and rapid tissue penetration would significantly contribute to the growth of the market. (mynewsdesk.com)
- So, the global market for aptamers is expected to raise, backed by technological advancements with respect to selection processes and synthesis. (prsync.com)
- We report the protocols for the synthesis and purification of Peptide Nucleic Acid (PNA) oligomers incorporating modified residues. (jove.com)
- PNAs can be synthesized relatively easily by the manual Boc-chemistry solid-phase peptide synthesis method. (jove.com)
- Chemical aptamer synthesis enables rapid, low-cost production of new batches with low lot-to-lot variability. (cambio.co.uk)
- Aptabodies are formed by hybridization of two DNA aptamers and allow detection of human thrombin effectively compared to traditional aptamers. (openpr.com)
- Aptamers are generated quickly and applied in the inhibition, characterization of proteins and for particular detection. (prsync.com)
- The huge potential of aptamer-nanoparticle-based detection of cancer cells and biomedical in - vivo imaging has been shown, with some examples. (smw.ch)
- They can additionally include organic dyes for optical detection, just as functional groups for their conjugation with aptamers [52, (smw.ch)
- Aptamer-modified nanoparticles for medical applications are developed mostly for the detection of analytes, for biomedical in-vivo imaging and for in-vivo drug delivering. (smw.ch)
- We aim to develop aptamers for detection of multiple bacterial proteins simultaneously which is expected to lead a more robust test," said Tarun Sharma, a member of the research team. (thehindubusinessline.com)
- The group used aptamer-based test also for detection of pleural TB, the second most prevalent form of extrapulmonary TB. (thehindubusinessline.com)
- From purification to imaging to detection/quantification, aptamers are enabling the use of traditional methods in new and different ways. (cambio.co.uk)
- Biotinylated aptamer was used for detection along with a streptavidin-HRP / TMB enzyme-substrate system. (cambio.co.uk)
- Following incubation with sample and appropriate wash steps, bound analyte is often detected with a FITC-labelled detection aptamer and the use of an anti-FITC-HRP / TMB enzyme-substrate system for colorimetric detection. (cambio.co.uk)
- One of these is a potent inhibitor of E2F binding activity in vitro and in mammalian fibroblasts, blocks cells in G1, and the free variable region from this aptamer has the same effect. (nih.gov)
- Aptamers were selected with the optimized magnetic epoxy beads method described in the Supplementary Material and used in vitro at 150 nmol/L unless otherwise specified. (aacrjournals.org)
- Several unique properties of aptamers make them attractive tools for use in a wide array of molecular biology applications and, moreover, as potential pharmaceutical agents. (mdpi.com)
- 1. A drug delivery composition which is suitable for transmucosal delivery and which comprises a plurality of microspheres adapted to gel in contact with the mucosal surface and active drug associated with each microsphere, the composition being substantially free of an enhancer, characterised in that the drug is for systemic delivery and is a peptide having a maximum molecular weight of 6000, and the microspheres comprise starch, gelatin, collagen or dextran. (google.es)
- 9. A drug delivery composition according to claim 1 wherein the peptide has a molecular weight of at least 1000. (google.es)
- By covering the full spectrum of topics relevant to peptide drugs, this timely handbook serves as an introductory reference for both drug developers and biomedical researchers interested in pharmaceutically active peptides, presenting both the advantages and challenges associated with this molecular class. (researchandmarkets.com)
- Downstream-peptide RNAs overlap a predicted non-coding RNA called yfr6 that is over 200 nucleotides in length, but it was proposed that only the upstream region (corresponding to the Downstream-peptide motif) functions as an RNA structure. (wikipedia.org)
- A distinct predicted non-coding RNA called yfr14 overlaps both yfr6 and Downstream-peptide RNAs. (wikipedia.org)
- It was hypothesized that Downstream-peptide RNAs correspond to riboswitches, based on multiple lines of evidence. (wikipedia.org)
- This, and other evidence, suggests that glnA RNAs are riboswitches, and their structural similarity to Downstream-peptide RNAs in turn suggests that Downstream-peptide RNAs are also riboswitches. (wikipedia.org)
- Your goal will be to explore what experimental parameters affect the enrichment of a heme-binding RNA aptamer from a mixture of heme-binding and non-binding RNAs. (mit.edu)
- Global Aptamers Market Technology Trend Analysis Market Report 2013-2018: Therapeutics, Diagnostics, Biosensors, Biomarker/Drug Discovery & Ap. (bio-medicine.org)
- Diagnostics segment, accounted for more than 30.0% share of global aptamer market in 2016, in terms of value. (openpr.com)
- Middle East and Africa Aptamers Market by type - (Peptide aptamers, Nucleic Acid aptamers (DNA-Based Aptamers, RNA-Based Aptamers, and XNA-Based Aptamers)), by Application - (Therapeutics Development, Diagnostics, Research and Development), by End users. (emailwire.com)
- Aptamer technology applies in number of areas such as diagnostics, food safety, research applications and therapeutics in number of industrial sectors like pharmaceutical, research laboratories and biotechnology. (mynewsdesk.com)
- The launch of Macugen, the first aptamer drug and first diagnostic test for testing mycotoxins in grains has acknowledged the potential of aptamers in diagnostics and therapeutics. (prsync.com)
- This review article gives a brief overview of recent relevant research into aptamers and trends in their use in cancer diagnostics and therapy. (smw.ch)
- The main part of the article is dedicated to current developments of aptamer-modified nanoparticles and their use in cancer diagnostics and treatment. (smw.ch)
- A new method of labeling peptides and proteins with F-18 via a metal ligand" Abstract #384, J Nucl Med. (freepatentsonline.com)
- A new method of labeling peptides and proteins with F-18 via a metal ligand", Power Point Presentation, 55th SNM Annual Meeting, New Orleans, LA, Jun. (freepatentsonline.com)
- A New Method of Labeling Peptides with F-18 Via a Metal Ligand" Abstract #68, 19th Winter Fluorine Conference (Jan. 11-16, 2009) Abstract Book, p. 32. (freepatentsonline.com)
- A New Method of Labeling Peptides with F-18 Via a Metal Ligand" PowerPoint Presentation, 19th Winter Fluorine Conference, St. Pete Beach, FL, Jan. 13, 2009. (freepatentsonline.com)
- Cutting-edge and easy to use, Nucleic Acid and Peptide Aptamers: Methods and Protocols will provide researchers with an inspiring and helpful guide to the application of these compounds to their own distinct research issues. (springer.com)
- Peptide aptamers and the methods to produce cassettes including the aptamers and manipulating them, are described. (patentsencyclopedia.com)
- Obviously, it is time-consuming and costly to design aptamers for specific proteins using experimental methods. (biomedcentral.com)
- Methods for aptamer-based nanoparticle application have been developed, but mostly by use of a few aptamers. (smw.ch)
- A brief overview of aptamer use and advantages for just a few of the many potential aptamer methods is provided below. (cambio.co.uk)
- Aptamer performance has been demonstrated in a number of different methods. (cambio.co.uk)
- Several of the many methods being re-developed with aptamers are listed here. (cambio.co.uk)
Develop new aptamers2
- Major factors responsible for the dominance of this segment are efforts by several key players to develop new aptamers for research and development applications. (openpr.com)
- Thus, these types of collaboration will help companies to develop new aptamers hence drives the market growth. (mynewsdesk.com)
- http://www.researchandmarkets.com/research/tfh2m9/aptamers_market ) has announced the addition of the "Global Aptamers Market Technology Trend Analysis Market Report 2013-2018" report to their offering. (bio-medicine.org)
- The global aptamers market is valued at $287 million in 2013 and is expected to reach $2.1 billion by 2018. (bio-medicine.org)
- The conserved sites G 133 K 134 and D 138 K 139 R 140 of C-terminal SmpB were identified by interacting with N-terminal SN, and concurrently the residue K 62 of ArfA was recognized by interacting with the surface loop of the specific peptide aptamer PA-2. (frontiersin.org)
- However, the region has not developed any oligonucleotide specific guideline that can address the general requirements for the quality of aptamer clinical trials. (openpr.com)
- We describe a method to immobilize specific peptide aptamers on individual electrodes at the micrometer scale, together with a robust and label-free electronic sensing system. (biomedcentral.com)
- The features for aptamers are extracted from Pseudo K-tuple Nucleotide Composition (PseKNC) while the features for proteins incorporate Discrete Cosine Transformation (DCT), disorder information, and bi-gram Position Specific Scoring Matrix (PSSM). (biomedcentral.com)
- The development of more aptamers specific to cancer cells or marker proteins is urgently needed, especially for these applications. (smw.ch)
- Chemically modified Peptide Nucleic Acid (PNA) oligomers have been recently developed that can recognize RNA duplexes in a sequence-specific manner. (jove.com)
- Here we describe a new RNA aptamer specific for IL4Rα/CD124. (aacrjournals.org)
- 14. The system of claim 1, where the aptamer comprises a polynucleotide having a sequence selected from the group consisting of SEQ ID NOS. (freepatentsonline.com)
- 3. The peptide aptamer expression cassette of claim 1, further comprising a transcriptional terminator signal sequence. (patentsencyclopedia.com)
- This length provides enough flexibility for the peptide to assume various conformations, while reducing the probability of randomly creating a stop codon in the aptamer coding sequence. (patentsencyclopedia.com)
- Considering that not all DNA analogs of RNA aptamers show functionality, the correlation between DNA and RNA sequence and their structure and function requires further investigation. (wikipedia.org)
- Two anti-PSMA aptamers were designed such that each binding sequence could fold independently into its active conformation. (eurekaselect.com)