A CELL LINE derived from a PHEOCHROMOCYTOMA of the rat ADRENAL MEDULLA. PC12 cells stop dividing and undergo terminal differentiation when treated with NERVE GROWTH FACTOR, making the line a useful model system for NERVE CELL differentiation.
A serine endopeptidase that has specificity for cleavage at ARGININE. It cleaves a variety of prohormones including PRO-OPIOMELANOCORTIN, proluteinizing-hormone-releasing hormone, proenkephalins, prodynorphin, and PROINSULIN.
A CALCIUM-dependent endopeptidase that has specificity for cleavage at ARGININE that is near paired basic residues. It cleaves a variety of prohormones including PRO-OPIOMELANOCORTIN; PRORENIN; proenkephalins; prodynorphin; prosomatostatin; and PROINSULIN.
A serine endopeptidase found primarily in the EXTRACELLULAR MATRIX. It has specificity for cleavage of a variety of substrates including PRORENIN, pro-membrane type-1 matrix metalloproteinase, and NEURAL CELL ADHESION MOLECULE L1.
A usually benign, well-encapsulated, lobular, vascular tumor of chromaffin tissue of the ADRENAL MEDULLA or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of EPINEPHRINE and NOREPINEPHRINE, is HYPERTENSION, which may be persistent or intermittent. During severe attacks, there may be HEADACHE; SWEATING, palpitation, apprehension, TREMOR; PALLOR or FLUSHING of the face, NAUSEA and VOMITING, pain in the CHEST and ABDOMEN, and paresthesias of the extremities. The incidence of malignancy is as low as 5% but the pathologic distinction between benign and malignant pheochromocytomas is not clear. (Dorland, 27th ed; DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, p1298)
Proteolytic enzymes that are involved in the conversion of protein precursors such as peptide prohormones into PEPTIDE HORMONES. Some are ENDOPEPTIDASES, some are EXOPEPTIDASES.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a choline moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and choline and 2 moles of fatty acids.
NERVE GROWTH FACTOR is the first of a series of neurotrophic factors that were found to influence the growth and differentiation of sympathetic and sensory neurons. It is comprised of alpha, beta, and gamma subunits. The beta subunit is responsible for its growth stimulating activity.
In tissue culture, hairlike projections of neurons stimulated by growth factors and other molecules. These projections may go on to form a branched tree of dendrites or a single axon or they may be reabsorbed at a later stage of development. "Neurite" may refer to any filamentous or pointed outgrowth of an embryonal or tissue-culture neural cell.
Tumors or cancer of the ADRENAL GLANDS.
A proprotein convertase with specificity for the proproteins of PROALBUMIN; COMPLEMENT 3C; and VON WILLEBRAND FACTOR. It has specificity for cleavage near paired ARGININE residues that are separated by two amino acids.
An acidic protein found in the NEUROENDOCRINE SYSTEM that functions as a molecular chaperone for PROPROTEIN CONVERTASE 2.
Tumors or cancer of the PROSTATE.
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
Established cell cultures that have the potential to propagate indefinitely.
The scientific disciplines concerned with the embryology, anatomy, physiology, biochemistry, pharmacology, etc., of the nervous system.
A cell line derived from cultured tumor cells.
A genus of gram-positive, anaerobic, coccoid bacteria that is part of the normal flora of humans. Its organisms are opportunistic pathogens causing bacteremias and soft tissue infections.
The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
A publication issued at stated, more or less regular, intervals.
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).
Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)
A disturbance in the prooxidant-antioxidant balance in favor of the former, leading to potential damage. Indicators of oxidative stress include damaged DNA bases, protein oxidation products, and lipid peroxidation products (Sies, Oxidative Stress, 1991, pxv-xvi).
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Various fish of the family SALMONIDAE, usually smaller than salmon. They are mostly restricted to cool clear freshwater. Some are anadromous. They are highly regarded for their handsome colors, rich well-flavored flesh, and gameness as an angling fish. The genera Salvelinus, Salmo, and ONCORHYNCHUS have been introduced virtually throughout the world.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.
A telecommunication system combining the transmission of a document scanned at a transmitter, its reconstruction at a receiving station, and its duplication there by a copier.
A peptide which is a homopolymer of lysine.
Positively-charged atomic nuclei that have been stripped of their electrons. These particles have one or more units of electric charge and a mass exceeding that of the Helium-4 nucleus (alpha particle).
A phosphorothioate proposed as a radiation-protective agent. It causes splenic vasodilation and may block autonomic ganglia.
Individual's rights to obtain and use information collected or generated by others.
A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.
The evaluation by experts of the quality and pertinence of research or research proposals of other experts in the same field. Peer review is used by editors in deciding which submissions warrant publication, by granting agencies to determine which proposals should be funded, and by academic institutions in tenure decisions.
A mitochondrial myopathy characterized by slowly progressive paralysis of the levator palpebrae, orbicularis oculi, and extraocular muscles. Ragged-red fibers and atrophy are found on muscle biopsy. Familial and sporadic forms may occur. Disease onset is usually in the first or second decade of life, and the illness slowly progresses until usually all ocular motility is lost. (From Adams et al., Principles of Neurology, 6th ed, p1422)
A non-essential amino acid naturally occurring in the L-form. Glutamic acid is the most common excitatory neurotransmitter in the CENTRAL NERVOUS SYSTEM.
Drugs intended to prevent damage to the brain or spinal cord from ischemia, stroke, convulsions, or trauma. Some must be administered before the event, but others may be effective for some time after. They act by a variety of mechanisms, but often directly or indirectly minimize the damage produced by endogenous excitatory amino acids.
Oxygenated forms of carotenoids. They are usually derived from alpha and beta carotene.
Molecules or ions formed by the incomplete one-electron reduction of oxygen. These reactive oxygen intermediates include SINGLET OXYGEN; SUPEROXIDES; PEROXIDES; HYDROXYL RADICAL; and HYPOCHLOROUS ACID. They contribute to the microbicidal activity of PHAGOCYTES, regulation of signal transduction and gene expression, and the oxidative damage to NUCLEIC ACIDS; PROTEINS; and LIPIDS.
Peroxidase catalyzed oxidation of lipids using hydrogen peroxide as an electron acceptor.
Naturally occurring or synthetic substances that inhibit or retard the oxidation of a substance to which it is added. They counteract the harmful and damaging effects of oxidation in animal tissues.
A diverse class of enzymes that interact with UBIQUITIN-CONJUGATING ENZYMES and ubiquitination-specific protein substrates. Each member of this enzyme group has its own distinct specificity for a substrate and ubiquitin-conjugating enzyme. Ubiquitin-protein ligases exist as both monomeric proteins multiprotein complexes.
A highly conserved 76-amino acid peptide universally found in eukaryotic cells that functions as a marker for intracellular PROTEIN TRANSPORT and degradation. Ubiquitin becomes activated through a series of complicated steps and forms an isopeptide bond to lysine residues of specific proteins within the cell. These "ubiquitinated" proteins can be recognized and degraded by proteosomes or be transported to specific compartments within the cell.
The act of ligating UBIQUITINS to PROTEINS to form ubiquitin-protein ligase complexes to label proteins for transport to the PROTEASOME ENDOPEPTIDASE COMPLEX where proteolysis occurs.
A GLYCOINOSITOL PHOSPHOLIPID MEMBRANE ANCHOR-containing ephrin with a high affinity for the EPHA3 RECEPTOR. Early in embryogenesis it is expressed at high levels in the MESENCEPHALON; SOMITES; branchial arches, and LIMB BUDS.

The disulfide-bonded loop of chromogranin B mediates membrane binding and directs sorting from the trans-Golgi network to secretory granules. (1/4249)

The disulfide-bonded loop of chromogranin B (CgB), a regulated secretory protein with widespread distribution in neuroendocrine cells, is known to be essential for the sorting of CgB from the trans-Golgi network (TGN) to immature secretory granules. Here we show that this loop, when fused to the constitutively secreted protein alpha1-antitrypsin (AT), is sufficient to direct the fusion protein to secretory granules. Importantly, the sorting efficiency of the AT reporter protein bearing two loops (E2/3-AT-E2/3) is much higher compared with that of AT with a single disulfide-bonded loop. In contrast to endogenous CgB, E2/3-AT-E2/3 does not undergo Ca2+/pH-dependent aggregation in the TGN. Furthermore, the disulfide-bonded loop of CgB mediates membrane binding in the TGN and does so with 5-fold higher efficiency if two loops are present on the reporter protein. The latter finding supports the concept that under physiological conditions, aggregates of CgB are the sorted units of cargo which have multiple loops on their surface leading to high membrane binding and sorting efficiency of CgB in the TGN.  (+info)

Ral-specific guanine nucleotide exchange factor activity opposes other Ras effectors in PC12 cells by inhibiting neurite outgrowth. (2/4249)

Ras proteins can activate at least three classes of downstream target proteins: Raf kinases, phosphatidylinositol-3 phosphate (PI3) kinase, and Ral-specific guanine nucleotide exchange factors (Ral-GEFs). In NIH 3T3 cells, activated Ral-GEFs contribute to Ras-induced cell proliferation and oncogenic transformation by complementing the activities of Raf and PI3 kinases. In PC12 cells, activated Raf and PI3 kinases mediate Ras-induced cell cycle arrest and differentiation into a neuronal phenotype. Here, we show that in PC12 cells, Ral-GEF activity acts opposite to other Ras effectors. Elevation of Ral-GEF activity induced by transfection of a mutant Ras protein that preferentially activates Ral-GEFs, or by transfection of the catalytic domain of the Ral-GEF Rgr, suppressed cell cycle arrest and neurite outgrowth induced by nerve growth factor (NGF) treatment. In addition, Rgr reduced neurite outgrowth induced by a mutant Ras protein that preferentially activates Raf kinases. Furthermore, inhibition of Ral-GEF activity by expression of a dominant negative Ral mutant accelerated cell cycle arrest and enhanced neurite outgrowth in response to NGF treatment. Ral-GEF activity may function, at least in part, through inhibition of the Rho family GTPases, CDC42 and Rac. In contrast to Ras, which was activated for hours by NGF treatment, Ral was activated for only approximately 20 min. These findings suggest that one function of Ral-GEF signaling induced by NGF is to delay the onset of cell cycle arrest and neurite outgrowth induced by other Ras effectors. They also demonstrate that Ras has the potential to promote both antidifferentiation and prodifferentiation signaling pathways through activation of distinct effector proteins. Thus, in some cell types the ratio of activities among Ras effectors and their temporal regulation may be important determinants for cell fate decisions between proliferation and differentiation.  (+info)

Identification of a new Pyk2 target protein with Arf-GAP activity. (3/4249)

Protein tyrosine kinase Pyk2 is activated by a variety of G-protein-coupled receptors and by extracellular signals that elevate intracellular Ca2+ concentration. We have identified a new Pyk2 binding protein designated Pap. Pap is a multidomain protein composed of an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal SH3 domain. We demonstrate that Pap forms a stable complex with Pyk2 and that activation of Pyk2 leads to tyrosine phosphorylation of Pap in living cells. Immunofluorescence experiments demonstrate that Pap is localized in the Golgi apparatus and at the plasma membrane, where it is colocalized with Pyk2. In addition, in vitro recombinant Pap exhibits strong GTPase-activating protein (GAP) activity towards the small GTPases Arf1 and Arf5 and weak activity towards Arf6. Addition of recombinant Pap protein to Golgi preparations prevented Arf-dependent generation of post-Golgi vesicles in vitro. Moreover, overexpression of Pap in cultured cells reduced the constitutive secretion of a marker protein. We propose that Pap functions as a GAP for Arf and that Pyk2 may be involved in regulation of vesicular transport through its interaction with Pap.  (+info)

Intracellular sodium modulates the expression of angiotensin II subtype 2 receptor in PC12W cells. (4/4249)

Although the angiotensin II subtype 2 receptor (AT2-R) is expressed abundantly in the adrenal medulla, its physiological significance has not yet been determined. To obtain fundamental knowledge of the regulation of AT2-R expression in the adrenal medulla, we investigated the effects of modulating several ion channels on AT2-R expression in PC12W cells. Experiments were performed after 24 hours of serum depletion under subconfluent conditions. After 48 hours of treatment with various agonists or antagonists, the receptor density and mRNA level of AT2-Rs were quantified by 125I-[Sar1, Ile8]angiotensin II binding and Northern blot analysis. Ouabain (10 to 100 nmol/L) and insulin (10 to 100 nmol/L) dose-dependently increased receptor density and mRNA level. Analysis of the binding characteristics revealed that the ouabain-dependent increase in AT2-R levels was due to an increase in binding capacity without a change in the Kd value. These increases were blocked by lowering the Na+ concentration in the medium. A low concentration of the sodium ionophore monensin (10 nmol/L), the K+-channel blocker quinidine (10 micromol/L), and the ATP-sensitive K+-channel blockers tolbutamide (100 micromol/L) and glybenclamide (10 micromol/L) also significantly increased receptor density, but the ATP-sensitive K+-channel agonist cromakalim (100 micromol/L) decreased receptor density significantly (P<0.01). Nifedipine (10 micromol/L) decreased basal receptor density and completely blocked the increase in receptor density caused by these agents. The increase in receptor density caused by an increase in intracellular Na+ was accompanied by an increase in mRNA level, whereas the ATP-sensitive K+-channel blockers did not change mRNA level. Nifedipine slightly decreased mRNA level. These results suggest that AT2-R expression is sensitively regulated by intracellular cation levels. The change in intracellular Na+ level transcriptionally regulates AT2-R expression, whereas the K+-channel blocker-dependent upregulation appears to be at least in part posttranslational.  (+info)

Hyperoxia induces the neuronal differentiated phenotype of PC12 cells via a sustained activity of mitogen-activated protein kinase induced by Bcl-2. (5/4249)

We previously reported that rat pheochromocytoma PC12 cells express the neuronal differentiated phenotype under hyperoxia through the production of reactive oxygen species (ROS). In the present study, we found that in this phenotype, Bcl-2, an apoptosis inhibitor, affects mitogen-activated protein (MAP)-kinase activity, which is known as a key enzyme of the signal-transduction cascade for differentiation. When PC12 cells were cultured under hyperoxia, a rapid increase in MAP-kinase activity, including that of both p42 and p44, was observed. Although the activity level then decreased quickly, activity higher than the control level was observed for 48 h. PD98059, an inhibitor of MAP kinase, suppressed the hyperoxia-induced neurite extensions, suggesting the involvement of MAP-kinase activity in the mechanism of differentiation induced by ROS. An elevation of Bcl-2 expression was observed after culturing PC12 cells for 24 h under hyperoxia. This Bcl-2 elevation was not affected by treatment with PD98059, suggesting that it did not directly induce neurite extension under hyperoxia. However, the blockade of the Bcl-2 elevation by an antisense oligonucleotide inhibited the sustained MAP-kinase activity and neurite extensions under hyperoxia. Further, in PC12 cells highly expressing Bcl-2, the sustained MAP-kinase activity and neurite extensions under hyperoxia were enhanced. These results suggested that MAP kinase is activated through the production of ROS, and the subsequent elevation of Bcl-2 expression sustains the MAP-kinase activity, resulting in the induction of the neuronal-differentiation phenotype of PC12 cells under hyperoxia.  (+info)

Characterization of elementary Ca2+ release signals in NGF-differentiated PC12 cells and hippocampal neurons. (6/4249)

Elementary Ca2+ release signals in nerve growth factor- (NGF-) differentiated PC12 cells and hippocampal neurons, functionally analogous to the "Ca2+ sparks" and "Ca2+ puffs" identified in other cell types, were characterized by confocal microscopy. They either occurred spontaneously or could be activated by caffeine and metabotropic agonists. The release events were dissimilar to the sparks and puffs described so far, as many arose from clusters of both ryanodine receptors (RyRs) and inositol 1,4,5-trisphosphate receptors (InsP3Rs). Increasing either the stimulus strength or loading of the intracellular stores enhanced the frequency of and coupling between elementary release sites and evoked global Ca2+ signals. In the PC12 cells, the elementary Ca2+ release preferentially occurred around the branch points. Spatio-temporal recruitment of such elementary release events may regulate neuronal activities.  (+info)

Microvessels from Alzheimer's disease brains kill neurons in vitro. (7/4249)

Understanding the pathogenesis of Alzheimer's disease is of widespread interest because it is an increasingly prevalent disorder that is progressive, fatal, and currently untreatable. The dementia of Alzheimer's disease is caused by neuronal cell death. We demonstrate for the first time that blood vessels isolated from the brains of Alzheimer's disease patients can directly kill neurons in vitro. Either direct co-culture of Alzheimer's disease microvessels with neurons or incubation of cultured neurons with conditioned medium from microvessels results in neuronal cell death. In contrast, vessels from elderly nondemented donors are significantly (P<0.001) less lethal and brain vessels from younger donors are not neurotoxic. Neuronal killing by either direct co-culture with Alzheimer's disease microvessels or conditioned medium is dose- and time-dependent. Neuronal death can occur by either apoptotic or necrotic mechanisms. The microvessel factor is neurospecific, killing primary cortical neurons, cerebellar granule neurons, and differentiated PC-12 cells, but not non-neuronal cell types or undifferentiated PC-12 cells. Appearance of the neurotoxic factor is decreased by blocking microvessel protein synthesis with cycloheximide. The neurotoxic factor is soluble and likely a protein, because its activity is heat labile and trypsin sensitive. These findings implicate a novel mechanism of vascular-mediated neuronal cell death in Alzheimer's disease.  (+info)

Human nerve growth factor beta (hNGF-beta): mammary gland specific expression and production in transgenic rabbits. (8/4249)

Transgenic rabbits carrying gene constructs encoding human nerve growth factor beta (hNGF-beta) cDNA were generated. Expression of hNGF-beta mRNA was restricted to the mammary gland of lactating rabbits. Western Blot analysis revealed a polypeptide of 13.2 kDa in the milk of transgenic animals. hNGF-beta was purified from the milk by a two-step chromatographic procedure. Electrospray mass spectroscopy analysis of purified hNGF-beta depicted a molecular weight of 13,261 Da per subunit. The biological activity of the hNGF-beta was tested using PC12W2 cells and cultures of dorsal root ganglion neurons from chicken embryos. Crude defatted milk from transgenic animals and purified hNGF-beta demonstrated full biological activity when compared to commercial recombinant hNGF-beta.  (+info)

TY - JOUR. T1 - The roles of membrane estrogen receptor subtypes in modulating dopamine transporters in PC-12 cells. AU - Alyea, Rebecca A.. AU - Laurence, Stephanie E.. AU - Kim, Sung H.. AU - Katzenellenbogen, Benita S.. AU - Katzenellenbogen, John A.. AU - Watson, Cheryl S.. PY - 2008/8. Y1 - 2008/8. N2 - The effects of 17β-estradiol (E2) on dopamine (DA) transport could explain gender and life-stage differences in the incidence of some neurological disorders. We tested the effects of E2 at physiological concentrations on DA efflux in nerve growth factor-differentiated rat pheochromocytoma cells that express estrogen receptors (ER) α, ERβ, and G-protein coupled receptor 30 (GPR30), and DA transporter (DAT). DAT efflux was determined as the transporter-specific loss of 3H-DA from pre-loaded cells; a 9-15 min 10-9 M E2 treatment caused maximal DA efflux. Such rapid estrogenic action suggests a non-genomic response, and an E2-dendrimer conjugate (limited to non-nuclear actions) caused DA ...
NGF-differentiated PC12 cells have greater Ca2+ rises in response to depolarization than undifferentiated cells. Peak [Ca2+]cyt for PC12 cells loaded with fura-
We compared the role of tyrosine kinases in α1A-adrenergic receptor (AR) and growth factor receptor stimulation of mitogen-activated protein kinase pathways in PC12 cells. Norepinephrine (NE) (noradrenaline), epidermal growth factor (EGF) and nerve growth factor (NGF) caused different patterns of tyrosine phosphorylation in PC12 cells stably expressing α1A-ARs. NE increased tyrosine phosphorylation of focal adhesion-related kinase Pyk2 and a 70 kDa protein, probably paxillin, whereas EGF strongly stimulated tyrosine phosphorylation of the EGF receptor and cytokine-activated kinase Jak2. The EGF receptor inhibitor AG1478 inhibited activation of extracellular signal-regulated kinases (ERKs) by EGF but not by NE. EGF and NGF strongly activated tyrosine phosphorylation of Shc and caused association of Src-homology collagen (Shc) with growth-factor-receptor-bound protein 2 (Grb2); however, neither NE nor UTP caused substantial activation of the Shc/Grb2 pathway. NE, UTP, EGF and NGF all increased ...
Evidence suggests that regulated ubiquitination of proteins plays a critical role in the development and plasticity of the central nervous system. We have previously identified the ubiquitin ligase Praja1 as a gene product induced during fear memory consolidation. However, the neuronal function of this enzyme still needs to be clarified. Here, we investigate its involvement in the nerve growth factor (NGF)-induced differentiation of rat pheochromocytoma (PC12) cells. Praja1 co-localizes with cytoskeleton components and the neurotrophin receptor interacting MAGE homologue (NRAGE). We observed an enhanced expression of Praja1 after 3 days of NGF treatment and a suppression of neurite formation upon Praja1 overexpression in stably transfected PC12 cell lines, which was associated with a proteasome-dependent reduction of NRAGE levels. Our data suggest that Praja1, through ubiquitination and degradation of NRAGE, inhibits neuronal differentiation. The two murine isoforms, Praja1.1 and Praja1.2, appear to be
Rat pheochromocytoma-derived PC12 cells are an important cell model for studies of growth factor cell signaling pathways. PC12 has been used for a variety of studies where cell division and cell differentaiton have been induced through growth factor activation. Both NGF and FGF can promote neurite proliferation, while
Download Komatsu PC300-6,PC350-6 manuals. 3 x manuals., Komatsu PC300-6,PC300LC-6,PC350-6,PC350LC-6 manuals. x 3, Komatsu PC300-6,PC350-6 manuals. 2 manuals.Shop and O&M, Komatsu PC300-7EO,PC350-7EO manuals. 2 x manuals, ...
Signed-off-by: Igor Mammedov ,[email protected], --- - rebased on top of todays PCI tree to match modified tables --- tests/acpi-test-data/pc/APIC.cpuhp_sparse , Bin 0 -, 160 bytes tests/acpi-test-data/pc/DSDT.cpuhp_sparse , Bin 0 -, 5930 bytes tests/acpi-test-data/pc/SRAT.cpuhp_sparse , Bin 0 -, 264 bytes tests/bios-tables-test.c , 16 ++++++++++++++++ 4 files changed, 16 insertions(+) create mode 100644 tests/acpi-test-data/pc/APIC.cpuhp_sparse create mode 100644 tests/acpi-test-data/pc/DSDT.cpuhp_sparse create mode 100644 tests/acpi-test-data/pc/SRAT.cpuhp_sparse diff --git a/tests/acpi-test-data/pc/APIC.cpuhp_sparse b/tests/acpi-test-data/pc/APIC.cpuhp_sparse new file mode 100644 index 0000000000000000000000000000000000000000..67918d51b7b7fbd26aff57802b3cf358864fb36a GIT binary patch literal 160 zcmXAh%MpMe3`7G13?J,mR)CA1WX6lF*o~!Fij,,*a7f,E&Q5aM,P,xIDtldzrJSv1 z&J,^Gkm&6~&pn)8jo53Ale{s5)($!cstNn)[email protected] B4FLcE literal 0 HcmV?d00001 diff --git a/tests/acpi-test-data/pc/DSDT.cpuhp_sparse ...
NGF binds to and activates the protein tyrosine kinase gp 140prototrk. Expression of this receptor is required for at least some responses to NGF. Three outstanding issues are addressed in the present work. First, we determined whether expression of gp 140prototrk is required for all neuronal NGF responses. Second, we examined the role of gp 140prototrk in NGF binding and internalization. Third, we addressed the utility of NGF-nonresponsive PC12nnr5 cells for study of the NGF mechanism. In contrast to wild-type PC12 cells, PC12nnr5 cells do not express endogenous gp 140prototrk. We therefore asked whether they possess other defects that compromise NGF signaling pathways. To answer these questions, we transfected PC12nnr5 cells with a cDNA encoding full- length human gp 140prototrk and isolated cell lines permanently expressing the receptor. Introduction of trk rescued all of the many and varied NGF responses assessed, including enhanced protein tyrosine phosphorylation, induction of ...
Ares, GAMEPOWER gaming pc case, complete glass pc case, premium pc case, elite pc case, dream pc case, high performance pc case godlike pc case gamers dream
Yesterday, after a long string of complaints, Ubisoft announced that Tom Clancys The Division will be released on PC after all. The PC version of Watch Dogs, however, was announced with much less fanfare just after the game was revealed at E3 2012. Earlier this month at Ubisoft Montreal, I asked Watch Dogs Senior Producer Dominic Guay why that is, and also discussed the PC features and hardware
The global PC market is dwindling, and Windows 8 could be to blame, according to the beancounters at IDC.. The analyst firm released its rundown of global PC shipments on Tuesday, and the year-on-year double digit decline blows a cold wind for traditional PC makers like Dell and HP, and OS-slinger Microsoft.. It seems clear that the Windows 8 launch not only failed to provide a positive boost to the PC market, but appears to have slowed the market, Bob ODonnell, IDCs program vice president for clients and displays, said in a canned statement.. While some consumers appreciate the new form factors and touch capabilities of Windows 8, the radical changes to the UI, removal of the familiar Start button, and the costs associated with touch have made PCs a less attractive alternative to dedicated tablets and other competitive devices. Microsoft will have to make some very tough decisions moving forward if it wants to help reinvigorate the PC market.. Ouch.. Worldwide PC shipments for the first ...
Pc Interface Price - Select 2017 high quality Pc Interface Price products in best price from certified Chinese Pc Strand manufacturers, Pc Mainboard suppliers, wholesalers and factory on Made-in-China.com
pc freezing - posted in Windows XP, 2000, 2003, NT: Hello, hope you can help me. I have a pc that keeps freezing when i try to re-install win xp pro. to cut a long story short this pc is my friends, he tried to do a repair install of xp. he ended up with a dual boot system on one drive his original xp and an xp repair setup option. niether of which worked. so i took his pc away an formatted the hard drive and tryed to re-install xp but on setup screen pc freezes. ive checked the ha...
Ultra Slim Pc Case, Wholesale Various High Quality Ultra Slim Pc Case Products from Global Ultra Slim Pc Case Suppliers and Ultra Slim Pc Case Factory,Importer,Exporter at Alibaba.com.
**THIS is a PC Version** This PC Flash version (compatible with any brand of IWB) is to be used on a PC platform and can be used on a computer, IWB or PC tablet. Click Here To View Product Demo
**THIS is a PC Version** This PC Flash version (compatible with any brand of IWB) is to be used on a PC platform and can be used on a computer, IWB or PC tablet. Click Here To View Product Demo
Neuronal responses to extracellular matrix (ECM) constituents are likely to play an important role in nervous system development and regeneration. We have studi
Searching how to play WWE Mayhem on PC? MEmu App Play is your first choice, fully support WWE Mayhem on the best Android Emulator, better experience on PC.
from neuron import h h.load_file(stdrun.hoc) pc = h.ParallelContext() pc.gid_clear() pc.set_maxstep(10) section = h.Section() pc.set_gid2node(0, pc.id()) nc = h.NetCon(section(0.5)._ref_v, None, sec=section) nc.threshold = 0 nc.delay = 1 nc.weight[0] = 1 pc.cell(0, nc) pc.setup_transfer() h.stdinit() bbss = h.BBSaveState() pc.psolve(100) bbss.save_test ...
Cheap pc terminal server, Buy Quality terminal server client 2000 directly from China terminal electronic Suppliers: Mini PC thin client terminal with 16 bit graphic and 1280*1024
Red Dead Redemption 2 is finally coming to PC next week, but ahead of its arrival you can get a glimpse at the PC enhancements in the launch trailer above. Even on a regular PS4, Red Dead Redemption 2 is a looker, but for our wait we've been rewarded with a flashier version. Rockstar detailed the improvements along with the system requirements earlier in the month, and you can expect increased draw distance, improved tree, grass and fur textures, unlocked resolution and frame rate—there's a lot. Micah Bell in particular looks even more hideous now. I can't turn away. 
This PC Trainer simulates the behavior of the GTN/GDU 620 series interface and provides you with a safer environment to learn the basic operation of the system.. Due to its graphic memory, this system must be the only application running on your PC. In some cases, even though your computer meets the minimum PC requirements, the simulator may not function properly. This is due to the unique configuration of each computer and the extra processes and procedures that are utilized by that computer.. ...
PIM product data: HP Compaq dx2200 Minitower PC 3.4GHz 651 Mini Tower PC BEU376ET1AB PCs/workstations, compare, review, comparison, specifications, price, brochure, catalog, product information, content syndication, product info, product data, datasheet
ThinkVD Pocket PC Video Converter Convert any video formats to Pocket PC supported formats for playback on Pocket PC.(with edit video function) Publisher: ThinkVD Studio
Skleník LANITPLAST DNĚPR 3,14x12 m PC 6 mm. Skleník LANITPLAST DNĚPR 3,14x12 m PC 6 mm - Zahradní czleníky - Trefa Sport .czSkleník LANITPLAST DNĚPR 3,14x12 m PC 6 mmVlastnosti pr​o...
Downfall V2.0 - THETA (PC/FULL/2010) | tinyurl.com/pc55obq Downfall V2.0 - THETA (PC/FULL/2010) cb628b75e7 j cole like father like son %20comanche%204%20comple
LG Get product support for the LG WF-6022PC. Download WF-6022PC manuals, documents, and software. View WF-6022PC warranty information and schedule services.
2K has dated the PC demo of Spec Ops: The Line for June 19. The publisher has also revealed the system requirements, and confirmed that itll use Steamworks as DRM.
Play Lineage 2 Revolution on PC using MEmu Player Android Emulator! Featuring console quality graphics, large persistent world & massive PvP battles online.
A new No Mans Sky Beyond patch is live on PC, introducing fixes for a variety of issues. The patch will release on consoles soon
Comprehensive Healthcare Associates And Prevention Medicine Pc is provider located at Livonia MI. Comprehensive Healthcare Associates And Prevention Medicine Pc NPI is 1063537785 and taxonomy code is 207RH0003X.
です. #: src/data/pc+-file-reader.c:787 #, c-format msgid Invalid weight index %u. msgstr %u は無効な重み付けインデックスです. #: src/data/pc+-file-reader.c:857 src/data/sys-file-reader.c:1438 #, c-format msgid Invalid variable name `%s. msgstr 不正な変数名 `%s. #: src/data/pc+-file-reader.c:865 src/data/sys-file-reader.c:1453 #, c-format msgid Renaming variable with duplicate name `%s to `%s. msgstr 名前の変更で変数名 `%s と `%s が重複します. #: src/data/pc+-file-reader.c:877 #, c-format msgid Cannot weight by string variable `%s. msgstr 文字列変数 %s は重み付けには使えません. #: src/data/pc+-file-reader.c:975 src/data/sys-file-reader.c:2713 msgid File ends in partial case. msgstr ケースの一部でファイルが終わっています. #: src/data/pc+-file-reader.c:981 #, c-format msgid Case beginning at offset 0x%08x extends past end of data record at offset 0x%08x. msgstr オフセット ...
PC - PC - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector shRNA available for purchase from OriGene - Your Gene Company.
Hello, I am new and got lots of questions? The first one is; I have a k7t266pro mobo from MSI with 256 PC2100 DDR (no name), can I use pc 2700 DDR on this mobo and would I benifit on it by using this ( for ocing?). I also have a T bird 1400, GF MX200 32MB (crap), 30 GB Maxtor
I currently have the Extreme trial version which is so awesome and Im about to purchase the real product soon. I have also downloaded the PC Tune Up software than comes with the package since i wanna try it before the purchase. For some reason tho, after my tune up today, ZA recognized PC Tune UP as a virus called
McAfee Antivirus for PC helps you browse bank and shop safely online. McAfee Antivirus provides the protection needed for your PC with essentials like safe web browsing and firewall security.
Theres nothing quite like powering on a PC that youve assembled, especially considering how much money you saved and PC knowledge you gained during that sweet, sweet bonding time.
CobraTek PC Info scans your PC and get you access to a variety of services and programs. The application not only gets you precious information about your...
Complete service manual for SHARP PC7500 (HARD DISK MANUAL) delivered as a PDF file. Learn how to repair Your device, check parts catalog for part numbers. Use circuit diagram to locate and fix defect. Share with others how You fixed SHARP PC7500 (HARD DISK MANUAL) or ask for help.
Saygus V2 vs Sony Ericsson GC89 PCMCIA PC Cell WiFi Card. Compare the specs and features of the Saygus V2 and Sony Ericsson GC89 PCMCIA PC Cell WiFi Card.
for Pentium 4 CPUs with 533 / 400 MHz FSB and 8x / 4x / 2x AGP Bus plus Advanced ECC Memory Controller supporting PC2700 / 2100 /
cylonlover writes The Jack PC from Chip PC Technologies offers a neat and novel thin-client desktop computing solution where the computer doesnt just plug into the wall, it is the plug in the wall. Running on power provided by the ethernet cable that also connects it to the data center server, the...
Buy MAK M43C Cylinder Head,9pc... , Want to buy - Buy MAK M43C Cylinder Head,9pc..., Services, Services, Trade Leads, Trade Offers
Heys guys i was wondering if my gamming pc is good, and i still have over 150$ to spend so please give me any good suggestion on my pc.heres my build ;SSD ; OCZ SSD 2.5 SATA 60GB Vertex 2 285/275MB/S...
Command, &, Conquer:, Red, Alert, 3, (PC), : High Quality and unbiased Technology, PC and Console Hardware/Component Reviews and News.
LG LX550 vs Sony Ericsson GC89 PCMCIA PC Cell WiFi Card. Compare the specs and features of the LG LX550 and Sony Ericsson GC89 PCMCIA PC Cell WiFi Card.
There have been striking associations of cardiovascular diseases (e.g., atherosclerosis) and hypercholesterolemia with increased risk of neurodegeneration including Alzheimers disease (AD). Low-density lipoprotein (LDL), a cardiovascular risk factor, plays a crucial role in AD pathogenesis; further, L5, a human plasma LDL fraction with high electronegativity, may be a factor contributing to AD-type dementia. Although L5 contributing to atherosclerosis progression has been studied, its role in inducing neurodegeneration remains unclear. Here, PC12 cell culture was used for treatments with human LDLs (L1, L5, or oxLDL), and subsequently cell viability and nerve growth factor (NGF)-induced neuronal differentiation were assessed. We identified L5 as a neurotoxic LDL, as demonstrated by decreased cell viability in a time- and concentration-dependent manner. Contrarily, L1 had no such effect. L5 caused cell damage by inducing ATM/H2AX-associated DNA breakage as well as by activating apoptosis via lectin-like
The effect of nerve growth factor (NGF) on the expression of neurofilament and Thy-1 genes in rat PC12 pheochromocytoma cells was examined at both the transcriptional and post-transcriptional levels. Addition of NGF to cultured PC12 cells produced increases in mRNAs corresponding to the 68 kd neurofilament protein (NF68) and the Thy-1 glycoprotein within 24 h, with maximal effects of some 90- and 45-fold stimulation (relative to beta-actin mRNA) being observed after 12 and 4 days of treatment, respectively. In addition, transcriptional run-off analyses using isolated nuclei showed that NGF treatment resulted directly in 8- and 4-fold increases in the rate of NF68 and Thy-1 gene transcription. These gene activation events were independent of overt morphological differentiation of PC12 cells occurring both under conditions permissive and non-permissive for neurite outgrowth, and once established the new molecular phenotype was dependent upon the continued presence of NGF. This is the first ...
The p75 neurotrophin receptor (p75NTR) is a multifunctional molecule that interacts with various other receptors to control neuronal differentiation, migration, and survival. Pincheira et al. found with a two-hybrid screen with the death domain of p75NTR as bait that the transcription factor Sall2 interacted with the receptor. Sall2 was detected in most regions of the mouse brain and appeared to be present in neurons, not glial cells, where it colocalized with cells expressing p75NTR. Sall2 and p75NTR coimmunoprecipitated from mouse brain lysates, which suggests that the two proteins interacted in vivo. Studies in PC12 cells, a rat pheochromocytoma cell line that responds to nerve growth factor (NGF) and in which both p75NTR and the TrkA neurotrophin receptor are present, showed that under nonstimulated conditions Sall2 and p75NTR interacted and that stimulation with NGF promoted the dissociation of Sall2 from the receptor and its translocation into the nucleus (detected both biochemically and ...
To investigate the role of Akt in nerve growth factor (NGF)-induced neuronal differentiation, PC12 cells ectopically expressing wild-type or dominant-inhibitory forms of Akt were analyzed. NGF-induced neurite outgrowth was greatly accelerated in cell
TY - JOUR. T1 - The Wnt-1 proto-oncogene regulates MAP kinase activation by multiple growth factors in PC12 cells. AU - Pan, M. G.. AU - Wang, Y. H.. AU - Hirsch, D. D.. AU - Labudda, K.. AU - Stork, P. J.S.. PY - 1995/1/1. Y1 - 1995/1/1. N2 - PC12/Wnt-1 cells display morphological changes in response to stimulation by select growth factors but do not respond to NGF. Furthermore, stimulation by EGF can induce neuronal differentiation in these cells but not in wild type cells. We have found that in these cells, compared to wild type PC12 cells, FGF and EGF stimulation of MAP kinase activity is enhanced, while NGF stimulation of MAP kinase in diminished. Finally, in cells expressing Wnt-1, the effect of cyclic adenosine monophosphate (cAMP) on MAP kinase activation is reversed; cAMP stimulates MAP kinase in wild type PC12 cells but inhibits MAP kinase in PC12/Wnt-1 cells. These data suggest that Wnt-1 expression alters the specificity of growth factor signaling in neuronal cells.. AB - PC12/Wnt-1 ...
Purpose: Alzheimers Disease is a neurodegenerative disease resulting from over-production and neuronal accumulation of amyloid-beta proteins (Aβ40/Aβ42). The glycine residue at position 33 and histidine residues at positions 13 and 14 are involved with binding and internalization of these proteins, actions potentially inhibited by substituting or sterically hindering these residues with an antibody specific to positions 2-11 (IgG-4.1). Rat pheochromocytoma (PC12) cells differentiated with nerve growth factor were used as a neuronal model to determine whether substitution and/or antibody block amyloid-betas neuronal interactions. Methods: PC12 cells were incubated with fluorescein-labeled-amyloid-beta-40 (F-Aβ40) or substituted F-Aβ40 derivatives (F-Aβ40-H13,14G, F-Aβ40-H13,14G;G33A), with or without IgG-4.1. Cells were analyzed by flow cytometry. Results: PC12 cells incubated with F-Aβ40 and IgG-4.1, as well as cells incubated with substituted peptides, exhibit decreased mean fluorescence
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011] Product Name ...
he intracellular distribution of 125I-labeled nerve growth factor (NGF) in rat pheochromocytoma PC12 cells was studied by quantitative electron microscopic (EM) autoradiography and by subcellular fractionation. PC12 cells were grown as monolayer cultures in medium supplemented with serum in the presence of 125I-NGF. EM autoradiography showed that 125I-NGF was localized at the plasma membrane and cytoplasmic compartments but did not accumulate in the nuclear chromatin or in the nuclear membrane compartment of cells analyzed after 1 hr and 1, 2, and 8 d of incubation with 125I-NGF. 125I-NGF also was not detected in nuclear subcellular fractions prepared from cells grown in serum-supplemented medium either in suspension for 1 d or in monolayer cultures for 1 to 8 d. In contrast, and in confirmation of the results of Yankner and Shooter (Yankner, B. A., and E. M. Shooter (1979) Pro. Natl. Acad. Sci. U. S. A. 76: 1269-1273), about 60% of the cell-bound 125I-NGF was found in the nuclear pellet after ...
BodyBio PC Liquid Phosphatidylcholine, 8 fl oz Formulated with a 4:1 Ratio of Omega 6 to Omega 3 in liquid form. Of the tens of thousands of molecules that make up the life of a cell, Phosphatidylcholine (PC) stands apart; probably the most important one of all. BodyBio PC is made of only pure phospholipids which automatically form liposomes in the watery environment of the body, but it goes one important step further… it is also formulated with the essential lipids at a 4:1 ratio. Most PC currently available has an EFA ratio of ~12:1. BodyBio PC not only has a higher concentration of phospholipids, up to 66%, but also maintains EFA concentration at the preferred 4:1 ratio, a BodyBio exclusive. What is BodyBio PC? BodyBio PC is a special, highly concentrated mixture of phospholipids with the highest one being Phosphatidylcholine. The key fact is that PC declines with age and disease. What is the recommended dosage/administration? 1-2 teaspoons per day or as directed by your physician. ...
Download Komatsu PC130-6K, PC150LGP-6K Hydraulic Excavator Operation & Maintenance Manual Download (SN F30001 and up, 35001 and up), Komatsu PC130-6K, PC150LGP-6K Hydraulic Excavator Service Repair Manual INSTANT DOWNLOAD (SN: F30001 and up, 35001 and up), KOMATSU PC130-6K, PC150LGP-6K HYDRAULIC EXCAVATOR SERVICE REPAIR MANUAL + OPERATION & MAINTENANCE MANUAL DOWNLOAD, Komatsu PC130-6K, PC150LGP-6K Hydraulic Excavator Workshop Repair Service Manual, ...
Glucocorticoids, secreted by the adrenals in response to stress, profoundly affect structure and plasticity of neurons. Glucocorticoid action in neurons is mediated by glucocorticoid receptors (GR) that operate as transcription factors in the regulation of gene expression and either bind directly to genomic glucocorticoid response elements (GREs) or indirectly to the genome via interactions with bound transcription factors. These two modes of action, respectively called transactivation and transrepression, result in the regulation of a wide variety of genes important for neuronal function. The objective of the present study was to identify genome-wide glucocorticoid receptor binding sites in neuronal PC12 cells using Chromatin ImmunoPrecipitation combined with next generation sequencing (ChIP-Seq). In total we identified 1183 genomic binding sites of GR, the majority of which were novel and not identified in other ChIP-Seq studies on GR binding. More than half (58%) of the binding sites contained a GRE.
TY - JOUR. T1 - Up-regulation of Idh3α causes reduction of neuronal differentiation in PC12 cells. AU - Cho, Sun A.. AU - Seo, Min Ji. AU - Ko, Je Yeong. AU - Shim, Jung Hee. AU - Yoo, Jin. AU - Kim, Jung Hee. AU - Kim, Se Yoon. AU - Ryu, Na Kyung. AU - Park, Eun Young. AU - Lee, Han Woong. AU - Lee, Yeon Su. AU - Bahk, Young Yil. AU - Park, Jong Hoon. PY - 2010/5. Y1 - 2010/5. N2 - The PC12 is the widely used cell line to study neuronal differentiation. We had extensively investigated the details of protein expression in differentiated PC12 cells by proteomic analysis. The cells were incubated at the presence of nerve growth factor. We had analyzed the expression changes in the differentiating PC12 cells by 2-dimensional electrophoresis and the identification of the proteins using MALDI-TOF MS. By comparing expression pattern in the time course, we identified the candidate genes which are associated with neuronal differentiation. Among these genes, we performed real-time PCR analysis to ...
Once inside your computer, Smart PC Fix will program itself to start automatically when you restart your computer. Smart PC Fix will perform computer scanning and will display fake scanning reports which says your computer is infected with Virus and Trojans. Smart PC Fix will also produce many unwanted pop-ups which states your computer is deeply infected with malware and you will be forced to buy the removal software to remove these infections. One thing you should know that this pop-ups and virus scanning reports are all fake. These are some of the usual tricks done by hackers to steal your money. Smart PC Fix rouge program will use most of the computer resources and will make your computer unusable. Manual removal of Smart PC Fix rogue software is not recommended as it involves removing fake entries from computer windows registry. Editing windows registry is only advised for expert users. You should backup your windows registry before editing it. Any unwanted change in registry may result in ...
About hybrid PC. A hybrid PC is a 2-in-1 device that functions as a tablet as well as a laptop. Hybrid PC has a detachable screen. Based on the screen size, a hybrid PC is classified into three: 10-12 inches, 12-14 inches, and above 14 inches.. Browse Full Research Report With TOC On Global Hybrid PC Market 2015-2019 at: http://www.radiantinsights.com/research/global-hybrid-pc-market-2015-2019. Covered in this report. The report includes the present scenario and the growth prospects of the global hybrid PC market for the period 2015-2019. To calculate the market size, this report estimates revenue generated from the shipments of hybrid PCs to end-users.. The report covers the Americas, APAC, and EMEA; it also covers the market landscape and its growth prospects in the coming years. The report includes a discussion of the Key vendors operating in this market.. Key regions. • Americas. • APAC. • EMEA. See More Reports of This Category by Radiant ...
I am using your great package fviz_cluster to visualize my cluster partition object obtained from CLARA over PC1 (dim1) and PC2(dim2). But I want to visualize it with PC3 and PC4 as well. I tried using the argument choose.vars= c(PCA$scores[,Comp.3],PCA$scores[,Comp.4]) where PCA is my princomp object and PCA$scores[,Comp.3] is the PC3 scores for each of my observations in my dataset. However, I get an error message ...
We have all read different comments on this site about Prostate Cancer(PC), and how little a lot of people know about it, and that many think it is just a slow cancer that is a good type of cancer to have, and it sure dosent look like we have anything wrong with us if we have PC. As men, we dont push the issue like we should. Admit it guys, PC is affecting our lives, our families, and all those around us. It is time to stand up and face the world and say Ive got Prostate Cancer and I am going to kick its ***.. Women are blessed with Breast Cancer(BC), and men with Prostate Cancer(sarcasim). Take a look at the number of posts/comments on the BC discussion site, and the number of the posts/comments on the PC site. Are we unable to communicate, or are we embarressed, or just to plain dumb to discuss it. PC is not a sin to have. I do not wear it like a medal or badge of courage, but I do believe in talking to my friends and co-workers about it. It does not take to much to put a bug in someones ...
PRODUCT DESCRIPTION. AmiLife® Soya PhosphatidylCholine- 30 Powder is in De-Oiled/Powder form with min. concentration of PC as 30% Lecithin container with four Phospholipids- such as PC (Phosphatidylcholine), PI,(Phosphatidylinositol),PE (Phosphatidylethanolamine), PA. (Phosphatidylethanolamine) out of which PC is most important. Its, provide structural framework for the membrane and maintain permeability barrier, in Brain it is precursor to Acetylcholine, and important neurotransmitter involved in cognition and motor co-ordination. PC is the part of Bile complex that emulsifies fats to facilitate absorption of fats and fat soluble nutrients for digestion system. PC is plays a crucial role in repair of the cells in Liver, its also helps in keeping control of Cholesterol levels. It helps in proper Cognitive function and prevent diseases like memory loss, dementia and special Liver, Brain and heart, since it dissolves fats very rapidly.. PRODUCT APPLICATION AmiLife® Soya PhosphatidylCholine- 30 ...
hook pc to tv Casual Online dating Uncensored Image Movie Profiles.. hook pc to tv Find a Brand-new Fawk Buddy When. Sign up for At this point.. Naughty Sexual Encounters. hook pc to tvExhibit Your current Intercourse Fantasies In Discrete Activities. Sign up for Right now.. Transexual Connect Online dating Meet up with Crotch Keen Transsexuals For the Bumping Very good Moment. Enroll in Now.. hook pc to tv Find a Completely new Fawk Buddy When. Enroll in Today.. Cyber Sexual intercourse Chat rooms Fully Uncensored Cyber Sex Conversation. Sign up for Sexual intercourse Chat rooms. Always be Kinky.. ...
88Pcs white nylon, M3-0.5 threaded hex standoff assortment kit in a handy storage box Includes: Storage Box with latching lid 20pc. 20mm Screws 20pc. Nuts Females have internal thread 6pc. Female/Male: 6mm + 6mm male thread 6pc. Female/Male: 10mm + 6mm male thread 6pc. Female/Male: 15mm + 6mm male thread 6pc. Female/Male: 20mm + 6mm male thread 6pc. Female: 6mm (internal threaded) 6pc. Female: 10mm 6pc. Female: 15mm 6pc. Female: 20mm [WT:] .1
from-windows-store/ talking,,,tom,,,4,,,free,,,download,,,-,,,My,,,Talking,,,Angela:,,,Enjoy,,,a,,,pet,,,cat,,,without,,,ever,,, changing,,,a,,,litter,,,box!,,,,and,,,much,,,more,,,programs.,,,Download,,,,My,,,,Talking,,,,Tom,,,,2,,,,for,,,,Windows,,,,(7/8/Xp),,,,PC,,,,or,,,,Computer,,,, appsforpcon.com/talking-tom-gold-run-for-pc-windows-7810-and-mac/ Sep,,,,15,,,,,2014,,,,,,,,You,,,,can,,,,download,,,,Talking,,,,Tom,,,,2,,,,for,,,,PC,,,,running,,,,Windows,,,,XP/7/8,,,,and,,,,enjoy,,,,,,,, Dungeon,,,,Hunter,,,,4,,,,for,,,,Pc,,,,Download,,,,(,,,,Computer,,,,and,,,,Laptop,,,,).,,,,Talking,,,,Tom,,,,-,,,,Talking,,,,Tom,,,,and,,,,Friends https://my-talking-tom.jaleco.com/ Explore,,,,this,,,,endless,,,,runner,,,,and,,,,help,,,,TALKING,,,,TOM,,,,and,,,,TALKING,,,,ANGELA,,,, chase,,,,down,,,,the,,,,robber,,,,and,,,,get,,,,your,,,,gold,,,,,,,,Download,,,,one,,,,of,,,,our,,,,games,,,,today!.,,,,My,,,Talking,,,Tom,,,for,,,PC,,,or,,,Computer,,,Free,,,Download,,,-,,,Blogarama ...
China Industrial Pc Battery manufacturers - Select 2017 high quality Industrial Pc Battery products in best price from certified Chinese Industrial Touch Pc manufacturers, Mini Industrial Pc suppliers, wholesalers and factory on Made-in-China.com
ATi_Elite1505d ago (Edited 1505d ago ). So yes Im a PC Elitist Jerk because I was one of the millions of Gamers who saw the Glory of the PC and kept on buying PC Games off a self in the Janitors Closet, kept on posting advice in threads on improvements, and kept on sitting alone in a dark room on a 56k dial-up connection because myself and Million of others somehow Knew that this PC Gaming thing was good, it was Ours and it was customizable and it was OPEN to all ...
Tritium: Ultimate Threadripper PC: Part 1 - Singularity Computers provides PC case mods services such as liquid-cooling products, custom built pc, Pc Rebuild & upgrade, custom built components, custom cases and many more.
Space Rocket - Star World is a Casual Game, Download Space Rocket - Star World For PC from the direct download link on PC Grim. Space Rocket - Star World is a
Page 1 of 3 - PC shuts down in safe mode [Closed] - posted in Virus, Spyware, Malware Removal: My PC randomly started shutting down on me in regular mode and safe mode. The PC occasionally will shut down in regular mode but if I enter safe mode and attempt to run virus removal programs it will shut down after a just few seconds.
PC(16:1(9Z)/22:1(9Z)) is a phosphatidylchloline (PC). It is a glycerophospholipid in which a phosphorylcholine moiety occupies a glycerol substitution site. As is the case with diacylglycerols, phosphatidylcholines can have many different combinations of fatty acids of varying lengths and saturation attached to the C-1 and C-2 positions. PC(16:1(9Z)/22:1(9Z)), in particular, consists of one 9Z-hexadecenoyl chain to the C-1 atom, and one 9Z-docosenoyl to the C-2 atom. In E. coli, PCs can be found in the integral component of the cell outer membrane. They are hydrolyzed by Phospholipases to a 2-acylglycerophosphocholine and a carboxylate ...
Alibaba.com offers 230 pc case accessories for ipad mini products. About 66% of these are mobile phone bags & cases. A wide variety of pc case accessories for ipad mini options are available to you, such as plastic, pu, and silicon.
PIM product data: StarTech.com 80x25mm Ever Lubricate Bearing PC Computer Case Fan w/ LP4 Ever Lubricate Bearing PC Case Fan - Case fan - 80 mm FANBOXSL computer cooling components 80mm, 2000 RPM, 24.81 CFM, 23 dBA, 12V, 0.96W, compare, review, comparison, specifications, price, brochure, catalog, product information, content syndication, product info, product data, datasheet
HerbsPro offers best Razor Triple Replacement Blades 4 pc, 1 pack by Preserve that are produced according to manufacturing standards set by regulators at everyday low prices. Shop Razor Triple Replacement Blades 4 pc, 1 pack by Preserve Online at HerbsPro.
PC Power Light Mod. This must be the saddest end of the PC modding biz, really it must. Cant get it together to tweak your case, keyboard or motherboard performance? Then why not mess with your power light so it blinks at you. Be still my beating heart. $9.99. Price includes a commemorative pocket pen protector case and a set of broken nerd glasses.. Give your PC a bit of bling with the easy to install Power LED Mod. This small module plugs directly into your motherboard and lets you customize the Power LED on the front of your PCs case. Get rid of that lame, always-on LED and mix it up with one of the 8 styles available on the Power LED Mod. Make your Power LED pulsate, simulate hard disk activity, fade in, fade out, and more!. ...
You want to connect your All-in-One PC to a set top box for watching TV without windows login.. If your set top box if of HD one then you can connect your PC to the box via HDMI cable to HDMI ports.I couldnt make sure that whether the PC has HDMI port or not.. You cannot use an external USB TV tuner without windows login as they require some driver(s) to be installed for use.. ...
For MX vs. ATV Reflex on the PC, GameFAQs has 48 achievements. MX vs ATV Unleashed Game Free Download PC Game highly compressed setup.MX vs ATV Unleashed Game - PC Download - Full Version G
For PC on the PC, a GameFAQs message board topic titled I just got a booster pack for a game I havent played for days and im not lvl 10.
Want to work at Community Care Physicians PC? Apply for Community Care Physicians PC jobs, learn about the culture, read reviews and more. Find Community Care Physicians PC careers in your area today!
For PC on the PC, a GameFAQs message board topic titled US Government is removing Kaspersky from their PCs and Bestbuy bans sales - Page 7.
Mani inhibits neurite outgrowth. Expression of Mani in PC12 cells inhibits Ngf (100 ng/ml)-induced neurite outgrowth and modulates the expression of pivotal cel
Within our archive we collect a lot of software including: Advanced SystemCare: Keep your PC being error-free and running at the peak performance. Ninja TuneUp: PC Speed Booster and Windows Registry Cleaner RegSweep: RegSweep is the latest in PC error diagnostic and repair. Scan Your PC For Free! RegClear: Repair your PC with RegClear Registry Cleaner Advanced-PCCare: Advanced-PCCare 2.1 makes your pc smooth, faster clean, and optimized. SystemCare: SystemCare boosts up the overall speed and performance of your PC instantly. USSystem-Care: USSystem-Care boosts up the computer speed and overall performance instantly. XP Registry Cleaner: get rid of the bloat in Windows registry and achieve a leaner, faster system ; these are very useful for free registry cleaner) ...
The Neurite Outgrowth Application Module for MetaXpress® software is designed for the analysis of neurite outgrowth assays. The module helps standardize results compared to traditional methods. Using a nuclear stain is beneficial to identify cell bodies in some cell types. With the flexibility of MetaXpress software, researchers are able to choose whether or not to use nuclear stains. Learn more
Download One Line : Single Stroke Drawing on Windows PC. Learn how to Install and run One Line : Single Stroke Drawing on PC (Windows 7/8/10). The best brain-training puzzle, free to play with tons of levels!
OCZ Technology Launches the PC2-8000 Titanium Alpha VX2 Series Sunnyvale, CA - June 29, 2006 - OCZ Technology Group, a worldwide leader in innovative, ultra-high performance and high reliability memory, today announced the PC2-8000 Titanium Alpha VX2 Edition, a special extremist series of modules...
the basic information about Phosphatidylcholine(PC) produced in China and supplied by Orison chemicals Limited, high quality and bulk quantity of Phosphatidylcholine(PC) from soy
Shop HP 4GB PC312800 (DDR31600 MHz) SODIMM Memory, 4 GB (1 x 4 GB), DDR3 SDRAM, (B4U39AA) at Staples. Choose from our wide selection of HP 4GB PC312800 (DDR31600 MHz) SODIMM Memory, 4 GB (1 x 4 GB), DDR3 SDRAM, (B4U39AA) and get fast & free shipping on select orders.
Our M02894 3-Section Foldable Transparent PC Protective Case for iPad Air must be an exquisite coat to decorate your device as well as offering utmost protection! The graceful and clear color makes it pretty attractive among thousands of protective cases in the current market while the 3-section foldable design allows you to enjoy wonderful movies, videos or other games conveniently in daily life. Also, durable PC and PU leather materials guarantee premium quality and long-lasting performance. Come to purchase one to get your iPad Air out of annoying fingerprints and scratches ...
Theres no need to put your needs on hold, the staff at Des Moiness Allergy-Asthma Associates PC is waiting for you. When you need medical attention, this facility is readily available to treat you and your symptoms. There is truly no better way to relax than with a treatment from Allergy-Asthma Associates PC so book your appointment today and head on over.
Rockstar ofera instrumente de editare in versiunea de PC a Grand Theft Auto V, astfel ca toti cei care fac screenshot-uri pe PC cu scene din joc vor putea sa creeze scene cu animale, obiecte sau caractere alese manual, precum si efecte de imagine in caz ca un cartier nu este pus intr-o lumina favorabila.. Rockstar Editor este un editor de film mai mult, ceea ce inseamna ca jucatorii vor putea sa isi reimagineze universul GTA V sau parti din el, cel putin. Urmariti clipul de prezentare de mai jos.. ...
When you talking a number of traders, providing students and graduates with valuable. KARACHI: The family members of peasant Mano Bheel are still missing. Protect Your Information RBC Capital Markets has strong measures in!. Scalping is the practice of buying and selling of any type of financial instrument in large quantities with in seconds. Feb 03, Its Official: B&N Has Ended Support for Nook PC they have some expanding spreads during high times. Its Official: B&N Has Ended Support for Nook PC and Evaluate REAPER for Free! CORRECTED national figures: Right 40.. It happens very often in forex but also in stock and. As a member of the investment research team the successful candidate will. Name: Sig, Interest, All connected to 1Gbitsec unlimited traffic, or other criteria. Step 1 Appe our free binary options course Who is this course for. Not just charting or trading software but a product that chose the Mac Apps computer. Sessions data.. Sering kali seorang marketer melakukan sesuatu yang ...
TDCPP was found to affect several neurodevelopmental processes in a neuronal cell line. PC12 cells showed decreased cell ... Studies in PC12 cells". Toxicology and Applied Pharmacology. 256 (3): 281-9. doi:10.1016/j.taap.2011.01.005. PMC 3089808. PMID ... When the TDCPP exposure began very early during embryogenesis, by the 2 cell stage, the developing embryos were more severely ... In a developing organism, these effects could change the way the brain cells communicate and function, resulting in permanent ...
Bai L, Swayne LA, Braun JE (January 2007). "The CSPalpha/G protein complex in PC12 cells". Biochem. Biophys. Res. Commun. 352 ( ... Cell. 123 (3): 383-96. doi:10.1016/j.cell.2005.09.028. PMID 16269331. S2CID 18173864. Swayne LA, Beck KE, Braun JE (September ... It is also found on secretory vesicles in endocrine, neuroendocrine and exocrine cells. This protein makes up ~1% of the ... 1999). "Mutational analysis of cysteine-string protein function in insulin exocytosis". J. Cell Sci. 112 (9): 1345-51. PMID ...
"Synaptotagmin IX regulates Ca2+-dependent secretion in PC12 cells". The Journal of Biological Chemistry. 277 (7): 4601-4. doi: ... Journal of Cell Science. 116 (Pt 21): 4307-18. doi:10.1242/jcs.00719. PMID 12966166. Morenilla-Palao C, Planells-Cases R, ... Journal of Cell Science. 116 (Pt 21): 4307-18. doi:10.1242/jcs.00719. PMID 12966166. \* Dubois T, Howell S, Zemlickova E, ...
Chapman ER, Blasi J, An S, Brose N, Johnston PA, Südhof TC, Jahn R (Aug 1996). "Fatty acylation of synaptotagmin in PC12 cells ... systemic mast cell disease, and a human mast cell leukemia cell line". The Journal of Histochemistry and Cytochemistry. 49 (3 ... O'Regan S, Diebler MF, Meunier FM, Vyas S (Jan 1995). "A Ewing's sarcoma cell line showing some, but not all, of the traits of ... Kimura N, Shiraishi S, Mizunashi K, Ohtsu H, Kimura I (Mar 2001). "Synaptotagmin I expression in mast cells of normal human ...
Vaudry, D.; Stork, PJ; Lazarovici, P; Eiden, LE (31 May 2002). "Signaling Pathways for PC12 Cell Differentiation: Making the ... Lazarovici, Philip; Fink, Donald (August 1999). "Heterologous Upregulation of Nerve Growth Factor-TrkA Receptors in PC12 Cells ... in PC12 Cells". Journal of Molecular Neuroscience. 54 (3): 574-585. doi:10.1007/s12031-014-0388-2. PMID 25078264. S2CID 1620005 ... in PC12 Cells". Journal of Molecular Neuroscience. 54 (3): 574-585. doi:10.1007/s12031-014-0388-2. PMID 25078264. S2CID 1620005 ...
Nitric Oxide mediates laminin-induced neurite outgrowth in PC12 cells. Experimental Cell Research, 260(2): 268-276. Weston, C.A ... Besides anchoring cells to the ECM, laminins are also involved in the signalling of cells and other components of the ECM. Even ... In cell adhesion laminin-111 and other isoforms are important proteins that anchor cells to the extracellular matrix (ECM). The ... However, when an isoform of actin, that cannot exit a cell's nucleus, is active, cells continue to grow and divide even when ...
Meng J, Casey PJ (November 2002). "Activation of Gz attenuates Rap1-mediated differentiation of PC12 cells". The Journal of ... regulating the differential activation of the mapk cascade by epidermal growth factor and nerve growth factor in PC12 cells". ... Experimental Cell Research. 257 (1): 172-9. doi:10.1006/excr.2000.4877. PMID 10854065. Kao S, Jaiswal RK, Kolch W, Landreth GE ... Cell. 65 (6): 1033-42. doi:10.1016/0092-8674(91)90555-D. PMID 1904317. S2CID 45725280. "Entrez Gene: RAP1GAP RAP1 GTPase ...
Connection between cell surface signaling and nucleus in PC12 cells". The Journal of Biological Chemistry. 272 (49): 31130-7. ... Nucleolin at the cell surface is the receptor for the Respiratory Syncytial Virus (RSV) fusion protein. Interference with the ... Said EA, Krust B, Nisole S, Svab J, Briand JP, Hovanessian AG (Oct 2002). "The anti-HIV cytokine midkine binds the cell surface ... Jordan P, Heid H, Kinzel V, Kübler D (Dec 1994). "Major cell surface-located protein substrates of an ecto-protein kinase are ...
Wu, Y., et al., Self-assembled IKVAV peptide nanofibers promote adherence of PC12 cells. Journal of Huazhong University of ... Early cell adhesion is very important for preventing cell degeneration; the longer cells are suspended in culture, the more ... The strength of cell attachment, cell migration rate, and extent of cytoskeletal organization formation is determined by the ... The IKVAV nanofibers promoted stronger cell adherence than the electrostatic attraction induced by poly-L-lysine, and cell ...
Jang JH, Aruoma OI, Jen LS, Chung HY, Surh YJ (February 2004). "Ergothioneine rescues PC12 cells from beta-amyloid-induced ... It is a metabolite of the antianginal drug molsidomine and acts by releasing NO from the endothelial cells nonenzymatically. It ... Wen TC, Rogido MR, Moore JE, Genetta T, Peng H, Sola A (October 2005). "Cardiotrophin-1 protects cortical neuronal cells ... also hyperpolarizes the cell membrane through influencing the sodium-potassium pump and thereby rendering it less responsive to ...
Shin, J. S.; Kim, K. T.; Lee, M. K. (Mar 1998). "Inhibitory Effects of Bulbocapnine on Dopamine Biosynthesis in PC12 Cells". ...
2002). "Glycine transporter isoforms show differential subcellular localization in PC12 cells". J. Neurochem. 82 (1): 58-65. ...
"Receptor mechanisms mediating cyanide generation in PC12 cells and rat brain". Neurosci Res. 49 (1): 13-18. doi:10.1016/j. ... The beta cells of the pancreas in type 1 diabetes produce an excess of the gas, leading to the death of these cells and to a ... There is a theory that, in some nerve cell synapses, when long-term memories are being laid down, the receiving cell makes ... These attributes make nitric oxide ideal for a transient paracrine (between adjacent cells) and autocrine (within a single cell ...
"Neuroserpin regulates neurite outgrowth in nerve growth factor-treated PC12 cells". Journal of Neurochemistry. 82 (6): 1406-15 ... It is a serine protease (EC 3.4.21.68) found on endothelial cells, the cells that line the blood vessels. As an enzyme, it ... cDNA was cloned into a synthetic plasmid and initially expressed in E. coli cells, followed by yeast cells with successful ... Tissue plasminogen activator also plays a role in cell migration and tissue remodeling.[citation needed] Once in the body, tPA ...
Weber E, Jilling T, Kirk KL (March 1996). "Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells". The ... Weber E, Jilling T, Kirk KL (March 1996). "Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells". The ... "Expression and polarized targeting of a rab3 isoform in epithelial cells". The Journal of Cell Biology. 125 (3): 583-94. doi: ... localization and functional role of small GTPases of the Rab3 family in insulin-secreting cells". Journal of Cell Science. 109 ...
Weber E, Jilling T, Kirk KL (March 1996). "Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells". J. ... Cell. 96 (3): 363-74. doi:10.1016/S0092-8674(00)80549-8. PMID 10025402. S2CID 15162326. Jung YJ, Lee TH, Lee JY, Kim JH, Park ... Mol Cell Biol. 15 (3): 1137-43. doi:10.1128/MCB.15.3.1137. PMC 230335. PMID 7532276. "Entrez Gene: RAB3A RAB3A, member RAS ... Cell. Signal. 11 (10): 735-42. doi:10.1016/S0898-6568(99)00037-6. PMID 10574328. Clabecq A, Henry JP, Darchen F (2000). " ...
"Receptor mechanisms mediating cyanide generation in PC12 cells and rat brain". Neuroscience Research. 49 (1): 13-8. doi:10.1016 ... It has also been shown that, while stimulating muscarinic cholinergic receptors in cultured pheochromocytoma cells increases ... shown that neuronal production of HCN activates NMDA receptors and plays a role in signal transduction between neuronal cells ( ...
Weber, E; Jilling T; Kirk K L (March 1996). "Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells". J ... Weber E, Jilling T, Kirk KL (1996). "Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells". J. Biol. ... "Identification of a rabphilin-3A-interacting protein as GTP cyclohydrolase I in PC12 cells". Biochem. Biophys. Res. Commun. 205 ... Inagaki N, Mizuta M, Seino S (February 1995). "Cloning of a mouse Rabphilin-3A expressed in hormone-secreting cells". J Biochem ...
2002). "Glycine transporter isoforms show differential subcellular localization in PC12 cells". J. Neurochem. 82 (1): 58-65. ... Reye P, Penfold P, Pow DV (2001). "Glyt-1 expression in cultured human Müller cells and intact retinae". Glia. 34 (4): 311-5. ... 2005). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation". ... Cytogenet Cell Genet. 71 (3): 211. doi:10.1159/000134110. PMID 7587377. "Entrez Gene: SLC6A9 solute carrier family 6 ( ...
2002). "Neuroserpin regulates neurite outgrowth in nerve growth factor-treated PC12 cells". J. Neurochem. 82 (6): 1406-15. doi: ... "Neuroserpin regulates neurite outgrowth in nerve growth factor-treated PC12 cells". J. Neurochem. 82 (6): 1406-15. doi:10.1046/ ...
1998). "The transcriptional corepressor NAB2 inhibits NGF-induced differentiation of PC12 cells". J. Cell Biol. 142 (4): 1075- ... doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. v t e. ... 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635-48. ... Mol Cell Biol. 16 (7): 3545-53. doi:10.1128/MCB.16.7.3545. PMC 231349. PMID 8668170. Kirsch KH, Korradi Y, Johnson JP (Jul 1996 ...
Maruyama Y, Suzuki Y, Kazusaka A, Fujita S (June 2001). "Uptake of the dopaminergic neurotoxin, norsalsolinol, into PC12 cells ... via vesicular monoamine transporter and its secretion by membrane depolarization or purinoceptor stimulation in PC12 cells". ...
in an experiment with PC12 cells and its name is non-acronymic. VGF gene encodes a precursor which is divided by proteolysis to ... "Reciprocal Signaling between Glioblastoma Stem Cells and Differentiated Tumor Cells Promotes Malignant Progression". Cell Stem ... and paracrine roles in feedback loops between differentiated glioblastoma cells and glioblastoma-specific cancer stem cells, ... is detected in a subset of neurons in the central and peripheral nervous systems and specific populations of endocrine cells in ...
Meng, X; Shi, J; Peng, B; Zou, X; Zhang, C (2006). "Effect of mouse Sim2 gene on the cell cycle of PC12 cell". Cell Biology ... When the SIM2 gene is tranfected into PC12 cells, it effects the normal cycle of cell maturation. SIM2 inhibits the expression ... Comparing normal human breast cells to human breast cancer cells with immunohistochemical staining showed that SIM2s was ... which in turn inhibits the cell's ability to pass through the G1/S checkpoint and suppresses the cell's proliferation ability. ...
"Lack of effects of typical and atypical antipsychotics in DARPP-32 and NCS-1 levels in PC12 cells overexpressing NCS-1". ... "Lack of effects of typical and atypical antipsychotics in DARPP-32 and NCS-1 levels in PC12 cells overexpressing NCS-1". ... "Downregulation of the cAMP/PKA pathway in PC12 cells overexpressing NCS-1". Cellular and Molecular Neurobiology. 31 (1): 135-43 ... "Downregulation of the cAMP/PKA pathway in PC12 cells overexpressing NCS-1". Cellular and Molecular Neurobiology. 31 (1): 135-43 ...
August 2003). "IRAS, the human homologue of Nischarin, prolongs survival of transfected PC12 cells". Cell Death and ... Edwards L, Ernsberger P (August 2003). "The I(1)-imidazoline receptor in PC12 pheochromocytoma cells reverses NGF-induced ERK ... a novel protein that interacts with the integrin alpha5 subunit and inhibits cell migration". The Journal of Cell Biology. 151 ... In human cardiac tissue, this gene was found to affect cell growth and death while in neural tissue it affected neuronal growth ...
PFOSA was the most toxic perfluorinated compound in a study with PC12 cells. Concentrations ranged from 10 to 250 micromolar in ... Fields S (June 2008). "Brain Effects Stick Around: PFCs and Neural Cell Development". Environ. Health Perspect. 116 (6): A258. ...
2002). "Slp4-a/granuphilin-a regulates dense-core vesicle exocytosis in PC12 cells". J. Biol. Chem. 277 (42): 39673-8. doi: ... "Slp4-a/granuphilin-a inhibits dense-core vesicle exocytosis through interaction with the GDP-bound form of Rab27A in PC12 cells ... Cell. 16 (2): 470-82. doi:10.1091/mbc.E04-08-0685. PMC 545880. PMID 15563604. Ross MT, Grafham DV, Coffey AJ, et al. (2005). " ... Cell. Biol. 22 (15): 5518-26. doi:10.1128/MCB.22.15.5518-5526.2002. PMC 133943. PMID 12101244. Fukuda M, Kanno E, Saegusa C, et ...
"Protective effect of diallyl disulfide on oxidative stress-injured neuronally differentiated PC12 cells". Molecular Brain ... Diallyl disulfide affects cancer cells much more strongly than normal cells. It also results in a strong and dose-dependent ... Garlic therefore supports, for example, the detoxification function of liver cells in vitro and protects nerve cells from ... The latter is reduced by diallyl disulfide by assisting in the detoxification of the cell, as well as some other mechanisms. By ...
"Cathepsin X promotes 6-hydroxydopamine-induced apoptosis of PC12 and SH-SY5Y cells". Neuropharmacology. 82: 121-31. doi:10.1016 ... Kos J, Jevnikar Z, Obermajer N (April-June 2009). "The role of cathepsin X in cell signaling". Cell Adhesion & Migration. 3 (2 ... This gene is expressed ubiquitously in cancer cell lines and primary tumors and, like other members of this family, may be ... Obermajer N, Svajger U, Bogyo M, Jeras M, Kos J (November 2008). "Maturation of dendritic cells depends on proteolytic cleavage ...
Yankner, BA; Benowitz, LI; Villa-Komaroff, L; Neve, RL (1990). "Transfection of the human GAP-43 gene in PC12 cells: effects on ... She completed her PhD at MIT in cell biology in 1975. She then went to Harvard to conduct her postdoctoral research for three ... The laboratory also discovered direct evidence that the Gap-43 protein was important in the growth of the axons of nerve cells. ... After her participation in the landmark research reporting the first synthesis of mammalian insulin in bacterial cells, Villa- ...
Roskoski R, Roskoski LM (Jan 1987). "Activation of tyrosine hydroxylase in PC12 cells by the cyclic GMP and cyclic AMP second ... cell nucleus. • synaptic vesicle. • melanosome membrane. • terminal bouton. • perikaryon. • axon. • neuronal cell body. • ... eye photoreceptor cell development. • response to insecticide. • dopamine biosynthetic process from tyrosine. • oxidation- ... Steinacker P, Aitken A, Otto M (Sep 2011). "14-3-3 proteins in neurodegeneration". Seminars in Cell & Developmental Biology. 22 ...
... was found to induce tubule polymerization in neuronal cultures and to increase neurite growth in PC12 cells ... "Transient receptor potential M3 channels are ionotropic steroid receptors in pancreatic beta cells". Nature Cell Biology. 10 ...
Minneman KP; Lee D; Zhong H (2000). „Transcriptional responses to growth factor and G protein-coupled receptors in PC12 cells: ... alpha 1-Adrenergic receptor subtypes differentially control the cell cycle of transfected CHO cells through a cAMP-dependent ... Gonzalez-Cabrera PJ; Shi T; Yun J (2004). „Differential regulation of the cell cycle by alpha1-adrenergic receptor subtypes.". ... Kang SK; Yi KS; Kwon NS (2004). „Alpha1B-adrenoceptor signaling and cell motility: GTPase function of Gh/transglutaminase 2 ...
2000). „Transcriptional responses to growth factor and G protein-coupled receptors in PC12 cells: comparison of alpha(1)- ... 2003). „alpha 1-Adrenergic receptor subtypes differentially control the cell cycle of transfected CHO cells through a cAMP- ... Cell Genet. 66 (3): 170-1. PMID 8125015. doi:10.1159/000133693.. *. Forray C; Bard JA; Wetzel JM; et al. (1994). „The alpha 1- ... Cell Genet. 67 (2): 69-74. PMID 8039425. doi:10.1159/000133802.. CS1 одржавање: Експлицитна употреба et al. (веза) ...
... morphogenetic protein 1-activated latent complex that can modulate nerve growth factor-induced differentiation of PC12 cells". ... "Cell. 153 (4): 828-39. doi:10.1016/j.cell.2013.04.015. PMC 3677132. PMID 23663781.. ... cell development. • negative regulation of cell differentiation. • positive regulation of pathway-restricted SMAD protein ... GDF11 induces tumor suppressive properties in human hepatocellular carcinoma-derived cells, Huh7 and Hep3B cell lines, ...
... inside the channel pore to block the entry of positive ions to the cell therefore inhibiting cell depolarisation.[63][64] ... PC12) (Ki = 136 nM), and the serotonin transporter (Ki = 2,234 nM).[41] The study notably found Ki values of ,10,000 nM for the ... normally the receptor acts as an ion channel and there is an influx of positive ions through the channel to cause nerve cell ...
Shin, J. S.; Kim, K. T.; Lee, M. K. (1998). „Inhibitory Effects of Bulbocapnine on Dopamine Biosynthesis in PC12 Cells". ...
... was found to induce tubule polymerization in neuronal cultures and to increase neurite growth in PC12 cells ... December 2008). "Transient receptor potential M3 channels are ionotropic steroid receptors in pancreatic beta cells". Nature ... Cell Biology. 10 (12): 1421-30. doi:10.1038/ncb1801. PMID 18978782.. *^ Kliewer SA, Lehmann JM, Milburn MV, Willson TM (1999 ...
PC12) cell line. In this study, we assayed the cell viability, the percentage of lactate dehydrogenase released (% LDH released ... The spleen cells immune response to sheep red blood cells of C3H/HeJ mice fed a 20 g whey protein/100 g diet is substantially ... The plaque-forming cell response to sheep red blood cells was found to be enhanced in mice fed a formula diet containing 20 g ... Whey Protein Concentrate Promotes the Production of Glutathione (GSH) by GSH Reductase in the PC12 Cell Line After Acute ...
... the exocyst complex and microtubules and disruption of its GTPase activity promotes aberrant neurite sprouting in PC12 cells". ... "Molecular Biology of the Cell. 13 (10): 3532-45. PMC 129964. . PMID 12388755. doi:10.1091/mbc.E02-01-0042. ... The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1". DNA ... Nagata K، Asano T، Nozawa Y، Inagaki M (December 2004). "Biochemical and cell biological analyses of a mammalian septin complex ...
ATP and dopamine in NGF-differentiated rat pheochromocytoma PC12 cells". Eur. J. Neurosci. 30 (5): 756-68. doi:10.1111/j.1460- ... "Cell. 130 (6): 1095-107. doi:10.1016/j.cell.2007.07.035. PMC 3366687. PMID 17889652.. ... "Cell. 155 (7): 1624-1638. doi:10.1016/j.cell.2013.11.037. PMC 4076149. PMID 24360282.. ... In recent years, NAD+ has also been recognized as an extracellular signaling molecule involved in cell-to-cell communication.[ ...
2000). "Transcriptional responses to growth factor and G protein-coupled receptors in PC12 cells: comparison of alpha(1)- ... positive regulation of cell proliferation. • cell proliferation. • positive regulation of smooth muscle contraction. • signal ... cell-cell signaling. • DNA metabolic process. • multicellular organism development. • adenylate cyclase-activating adrenergic ... 2003). "alpha 1-Adrenergic receptor subtypes differentially control the cell cycle of transfected CHO cells through a cAMP- ...
Electrochemical quantification of transmitter concentration in single nanoscale vesicles isolated from PC12 cells. Faraday ... 胞漿顆粒(英语:Granule (cell biology)). 黑色素體 · 微粒體 · 過氧化物酶體 · 乙醛酸循環體 · 韋伯潘力氏小體(英语:Weibel-Palade body) ... A whole-cell electron tomography model of vacuole biogenesis in Arabidopsis root cells.. Nature plants. 2019-01, 5 (1): 95-105 ... doi:10.1016/j.cell.2010.05.030.. *^ Blagitko, N; Schulz, U; Schinzel, AA; Ropers, HH; Kalscheuer, VM
Binda AV، Kabbani N، Levenson R (2005). "Regulation of dense core vesicle release from PC12 cells by interaction between the D2 ... regulated secretion in permeable neuroendocrine cells". Cell. 70 (5): 765-75. PMID 1516133. doi:10.1016/0092-8674(92)90310-9. ...
1999). "The Csk homologous kinase associates with TrkA receptors and is involved in neurite outgrowth of PC12 cells.". J. Biol ... "The Csk homologous kinase associates with TrkA receptors and is involved in neurite outgrowth of PC12 cells". J. Biol. Chem. ( ... and are regulated by stem cell factor in the megakaryoblastic cell line MO7e.". Growth Factors 14 (2-3): 103-15. PMID 9255603. ... 1997). "Association of csk-homologous kinase (CHK) (formerly MATK) with HER-2/ErbB-2 in breast cancer cells.". J. Biol. Chem. ...
... and its metabolites on undifferentiated PC12 cells: A putative structure-toxicity relationship". Chemical Research in ... towards embryonic stem cell-derived cardiac and neural cells". Toxicology in Vitro. 24 (4): 1133-8. doi:10.1016/j.tiv.2010.03. ... isomers of MDA and MDMA on phosphatidyl inositol turnover in cultured cells expressing 5-HT2A or 5-HT2C receptors". ... induces fenfluramine-like proliferative actions on human cardiac valvular interstitial cells in vitro". Molecular Pharmacology ...
"Receptor mechanisms mediating cyanide generation in PC12 cells and rat brain". Neurosci Res. 49 (1): 13-18. doi:10.1016/j. ... It has also been shown that, while stimulating muscarinic cholinergic receptors in cultured pheochromocytoma cells increases ... shown that neuronal production of HCN activates NMDA receptors and plays a role in signal transduction between neuronal cells ( ...
"CART peptide stimulation of G protein-mediated signaling in differentiated PC12 cells: identification of PACAP 6-38 as a CART ... "Cell. 135 (5): 825-37. PMC 2614332 . PMID 19041748. doi:10.1016/j.cell.2008.09.059.. ... During waking, REM-on cells are inhibited by a subset of ARAS norepinephrine and serotonin neurons called REM-off cells.. ... muscle cell, or gland cell.[1] Neurotransmitters are released from synaptic vesicles in synapses into the synaptic cleft, where ...
2001). "HIV-1 Tat protein down-regulates CREB transcription factor expression in PC12 neuronal cells through a ... Cell Mol. Genet. 20 (2): 75-86. doi:10.1007/BF02290677. PMID 8009369. Bolger G, Michaeli T, Martins T, et al. (1993). "A family ... Cell. Biol. 13 (10): 6558-71. doi:10.1128/mcb.13.10.6558. PMC 364715 . PMID 8413254. Obernolte R, Ratzliff J, Baecker PA, et al ... 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. ...
Purified from Sparassis crispa Mediated Neuro-Protection against Glutamate-Induced Toxicity in Differentiated PC12 Cells". Int ... Kim (2012). "Sparassis crispa suppresses mast cell-mediated allergic inflammation: Role of calcium, mitogen-activated protein ...
Herling DJ, Mintz L, Cohn FG, Popeo PC (12 January 2014). "As Maine Goes, So Goes The Nation? Labeling for Foods Made with ... cell fusion, mutation breeding) or genetic engineering. Canadian law requires that manufacturers and importers submit detailed ...
"Tyrosine-O-sulfated proteins of PC12 pheochromocytoma cells and their sulfation by a tyrosylprotein sulfotransferase". J. Biol ... viral cell entrance, and other cell-cell and protein-protein interactions. Selection for specific tyrosine residues requires a ... has generated interest because of its role as the target protein for the viral entrance of HIV into cells. The importance of ...
"Multiple effector domains within SNT1 coordinate ERK activation and neuronal differentiation of PC12 cells". J. Biol. Chem. 276 ... "A role for a PDZ protein in the early secretory pathway for the targeting of proTGF-alpha to the cell surface". Mol. Cell. 3 (4 ... Blake DJ (2002). "Dystrobrevin dynamics in muscle-cell signalling: a possible target for therapeutic intervention in Duchenne ... Cell Biol. 150 (6): 1385-98. doi:10.1083/jcb.150.6.1385. PMC 2150701. PMID 10995443. Newey SE, Benson MA, Ponting CP, Davies KE ...
1995). "v-Crk modulation of growth factor-induced PC12 cell differentiation involves the Src homology 2 domain of v-Crk and ... 1996). "DOCK180, a major CRK-binding protein, alters cell morphology upon translocation to the cell membrane". Mol. Cell. Biol ... alters cell morphology upon translocation to the cell membrane". Mol. Cell. Biol. 16 (4): 1770-6. doi:10.1128/MCB.16.4.1770. ... 1994). "Expression of the v-crk oncogene product in PC12 cells results in rapid differentiation by both nerve growth factor- ...
"Slp4-a/granuphilin-a inhibits dense-core vesicle exocytosis through interaction with the GDP-bound form of Rab27A in PC12 cells ... Cell. 13 (5): 1735-49. doi:10.1091/mbc.01-12-0595. PMC 111140. PMID 12006666. Nagashima K, Torii S, Yi Z, Igarashi M, Okamoto K ... Cell. Biol. 22 (6): 1858-67. doi:10.1128/MCB.22.6.1858-1867.2002. PMC 135591. PMID 11865063. Strom M, Hume AN, Tarafder AK, ... Cell. 13 (5): 1735-49. doi:10.1091/mbc.01-12-0595. PMC 111140. PMID 12006666. Kuroda TS, Fukuda M, Ariga H, Mikoshiba K (2002 ...
Han X, Zhu S, Wang B, Chen L, Li R, Yao W, Qu Z (2014). "Antioxidant action of 7,8-dihydroxyflavone protects PC12 cells against ... Cell Press (2015). "Molecule found in tree leaves helps female mice combat weight gain; males unaffected". ScienceDaily. ... "Estrogenic and antiproliferative activities on MCF-7 human breast cancer cells by flavonoids". Cancer Lett. 130 (1-2): 209-16. ... structural requirements for antiproliferative activity on breast cancer cells". Bioorg. Med. Chem. Lett. 11 (24): 3095-7. doi: ...
This makes PC12 cells useful as a model system for neuronal differentiation and neurosecretion. Treatment of PC12 cells with ... Wikipedia articles containing the term "PC12 cells" [1] Ras function was partially elucidated using PC12 cell line [2], from ... been studied using this cell line to understand their effects on PC12 cells These types of studies show that use of PC12 cell ... "The PC12 cell as model for neurosecretion: PC12 cells as model for neurosecretion". Acta Physiologica. 192 (2): 273-285. doi: ...
I am looking for references regarding the PC-12 cell line. What is the history of this line? Where did these cells come from? ... PC-12 Cells. Michael I Sandstrom misandst at magnus.acs.ohio-state.edu Sun Oct 10 14:23:06 EST 1993 *Previous message: brain ... What does in-vitro electrophysiology et cetera really tell us about the neuron of the CNS? Is this cell line capable of much ...
PC12 cells were transfected with vector, wild type or mutant Ngb DNA (1.0 μg/106 cell) for 48 h and treated with H2O2 (0.1mM) ... PC12 cells were transfected with 0.5 μg, 1.0 μg and 2.0 μg/106 cells of Ngb, Ngb mutant or vector DNA for 48 h. Following ... PC12 cells were transfected with Ng siRNA or control siRNA for 48 h. Following transfection, the cells were treated with H2O2 ( ... Ngb protein expression and cell viability in PC12 cells after transfection of Ngb siRNA and treatment of H2O2 ...
... Michael Holloway mhollowa at ccnova.sunysb.edu Tue Jul 21 18:08:00 EST 1992 *Previous ... Ive learned then that the upshot is that successfully keeping PC12 cells going relies entirely on a qualitative feel for the ... This is of particular importance to me now because of the results Ive gotten in preliminary observations of PC12 cells ... Apparently, the way cell density is followed on a practical basis is to split the cells when a certain degree of confluence is ...
Gelsolin overexpression enhances neurite outgrowth in PC12 cells.. Furnish EJ1, Zhou W, Cunningham CC, Kas JA, Schmidt CE. ... Cells that were genetically modified to overexpress gelsolin have longer neurites and a greater neurite motility rate compared ... One actin accessory protein, gelsolin, is involved with remodeling the cytoskeleton, although its role in cell motility is ...
... Rae Rae Fri May 27 16:50:10 EST 1994 *Previous message: How to differentiate PC12 ... I intended to differentiate PC12 cell with NGF. I added NGF in culture , medium containing 5% horse serum/fetal bovine serum. ... Another problem might be your PC12 cell line- if theyve been carried too long and allowed to overgrow before splitting, then ... However, only ,small part of cells differentiated. How can I overcome this problem ? ,Is it necessary to change the medium to ...
PC12 cells synthesize catecholamines (primarily dopamine) and acetylcholine (ACh), store each in different granules and exhibit ... PC12 is a clonal line of rat pheochromocytoma (7). ... PC12 is a clonal line of rat pheochromocytoma (7). PC12 cells ... Howard B.D., Melega W.P. (1981) Acetylcholine Metabolism in PC12, A Clonal Cell Line on Secretory Cells. In: Pepeu G., Ladinsky ... PC12 Cell Synaptic Vesicle Incubation Buffer ATPase Inhibitor Chromaffin Granule These keywords were added by machine and not ...
... Ji Jia,1 Lei Zhang,2 Xiaolei Shi,3 Mingchun Wu,4 ... Ji Jia, Lei Zhang, Xiaolei Shi, et al., "SOD2 Mediates Amifostine-Induced Protection against Glutamate in PC12 Cells," ...
Determination of Mammalian Cell Counts, Cell Size and Cell Health Using the Moxi Z Mini Automated Cell Counter, Spatial and ... pc12 cells include Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell ... Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact, In Vivo Single-Molecule ... Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5, Experimental Methods for Testing the Effects of ...
... dimeric proteins distributed widely among eukaryotic cells. This protein family binds to a variety of proteins in a ... PC12 Cell Tyrosine Hydroxylase Sepharose Bead Signal Transduction Kinase Major Ligand These keywords were added by machine and ... Ichimura T., Itagaki C., Wakamiya A., Isobe T. (2002) Interaction of Tyrosine Hydroxylase and 14-3-3 Proteins in PC12 Cells. In ... Interaction of Tyrosine Hydroxylase and 14-3-3 Proteins in PC12 Cells. ...
... cells treated with 6-OHDA (50 μM) for 1 hour, Group 2: cells treated with 6-OHDA (150 μM) for 1 hour, Group 3: cells treated ... Therefore, preexposure of PC12 cells to low 6-OHDA concentration can protect against subsequent toxic insults of 6-OHDA and OA ... Oleanolic Acid Enhances the Beneficial Effects of Preconditioning on PC12 Cells. Babongile C. Ndlovu, Willie M. U. Daniels, and ... In preconditioned cells, we found that cell viability remained high following exposure to 6-OHDA (150 μM). OA treatment ...
Cell survival in both untreated and nerve growth factor (NGF)-treated PC12 cells was inhibited by down-regulation of SOD1, with ... Down-regulation of copper/zinc superoxide dismutase causes apoptotic death in PC12 neuronal cells. C M Troy and M L Shelanski ... We have approached this problem by specifically inhibiting the synthesis of SOD1 in cultured PC12 cells with antisense ... Down-regulation of copper/zinc superoxide dismutase causes apoptotic death in PC12 neuronal cells ...
This agent induced a dose- and time-dependent cell death in neuronal PC12 cells. Cells treated with this agent showed condensed ... PC12 cells induced to differentiate with nerve growth factor were used to study the neurotoxicity of 25-OH-cholesterol. ... This agent induced a dose- and time-dependent cell death in neuronal PC12 cells. Cells treated with this agent showed condensed ... In contrast to observations made in other cell types, whole-cell patch clamp recording of neuronal PC12 cells revealed that ...
Using PC12 cell model, we showed that Glu and Hcy provoked a huge amount of reactive oxygen species (ROS) production, causing ... the damages caused by Glu and Hcy to PC12 cells can be alleviated by the potent antioxidant ATX. ... We hypothesize that Glu and Hcy could damage neuronal cells, while astaxanthin (ATX) could be beneficial to alleviate the ... Source of Cell Line. The cell line PC12 cells (BCRC 6008), originating from the pheochromocytoma on the rat adrenal gland, was ...
... and percent of bipolar cells and node/primary neurite ratios were used to assess the growth and complexity of PC12 cells ... We observed that the oligomeric form of insulin impaired the growth and complexity of PC12 cells compared to other forms. ... our data suggest that the lower surface tension of oligomers and their perturbation affects the morphology of PC12 cells, ... toxicity of the different forms of insulin we monitored morphological alterations of the differentiated neuron-like PC12 cells ...
... the effects of ablated S100A1 expression on neurite organization and microtubule/tubulin levels in PC12 cells were examin ... PC12 Cells. Rats. Recombinant Proteins / metabolism. S100 Proteins. Signal Transduction. Subcellular Fractions / metabolism. ... In summary, ablation of S100A1 expression in PC12 cells results in increased tubulin levels, altered neurite organization, and ... the effects of ablated S100A1 expression on neurite organization and microtubule/tubulin levels in PC12 cells were examined. A ...
PC12 cells have caveolae that contain TrkA. Other Titles: Caveolae-disrupting drugs inhibit nerve growth factor-induced, but ... Here we demonstrate the presence of caveolae in PC12 cells and their involvement in NGF signaling. Our results showed the ... In contrast, pretreatment of PC12 cells with these drugs inhibited the NGF- but not the epidermal growth factor-lnduced MAPK ... Nerve growth factor (NGF) induces survival and differentiation of the neural crest-derived PC12 cell line. Caveolae are ...
... after 3 days of NGF treatment and a suppression of neurite formation upon Praja1 overexpression in stably transfected PC12 cell ... Here, we investigate its involvement in the nerve growth factor (NGF)-induced differentiation of rat pheochromocytoma (PC12) ... cells. Praja1 co-localizes with cytoskeleton components and the neurotrophin receptor interacting MAGE homologue (NRAGE). We ...
Ahnert-Hilger, G.; Gratzl, Manfred (1987): Further Characterization of Dopamine Release by Permeabilized PC 12 Cells. In: ... Rat pheochromocytoma cells (PC 12) permeabilized with staphylococcal α-toxin release [3H]dopamine after addition of micromolar ... dopamine release from digitonin-permeabilized PC 12 cells. Permeabilization with α-toxin or digitonin and stimulation of the ... OH groups are not necessary for exocytosis in permeabilized PC 12 cells. ...
... on cell survival and mitochondria functioning in intact cells under conditions of undergoing cell divisions. Because after 48h ... on cell survival and mitochondria functioning in intact cells under conditions of undergoing cell divisions. Because after 48h ... Therefore, we applied inducible PC12 cell model of HD to determine the relationship between the effect of expression of wild ... Therefore, we applied inducible PC12 cell model of HD to determine the relationship between the effect of expression of wild ...
Stimulation of Noradrenaline Release by T-588, a Cognitive Enhancer, in PC12 Cells * * Maekawa Mutsuko MAEKAWA Mutsuko ... Effects of protein kinase C and A activation on ATP-stimulated release of [^3H]noradrenaline from PC12 cells ODA H. ... Neuritic growth from a new subline of PC12 pheochromocytoma cells : cyclic AMP mimics the action of nerve growth factor KATOH- ... Regulation of neurosecretory habituation in PC12 cells: parallel pathways used by CAMP and calcium MARTIN P. T. ...
Home/ Forums/ Neuroscience: Neurochemistry/ Apply lipid solution into PC12 cells. Apply lipid solution into PC12 cells. 2 posts ... Apply lipid solution into PC12 cells I am working with the anti-inflammatory property of emu oil. I have a problem in applying ... the lipid into the pc12 cells. Is there any method i can use to dissolve the lipid to induce the pc12 cells? thanks. ... Mechanistic studies of the uptake were conducted in PC12 cells. Incorporation of SP600125 afforded 8.2 fold greater solubility ...
Cell Culture and Tissue Engineering. Differentiation of PC12 cells in three-dimensional collagen sponges with micropatterned ... Limiting cell aggregation during mesenchymal stem cell expansion on microcarriers Next article in issue: Limiting cell ... PC12 cells were cultured in the NGF-micropatterned collagen sponges and showed micropatterned neurite outgrowth. The neurite ... The micropattern of the NGF and the neurite network of the PC12 cells can be manipulated by controlling the micropattern of the ...
... of the cell-bound 125I-NGF was found in the nuclear pellet after cell fractionation if the cells had been kept previously in ... PC12 cells were grown as monolayer cultures in medium supplemented with serum in the presence of 125I-NGF. EM autoradiography ... Internalization of nerve growth factor by pheochromocytoma PC12 cells: absence of transfer to the nucleus. H Rohrer, T Schafer ... The ultrastructure of PC12 cells grown under such conditions, however, revealed signs of varying degrees of damage. ...
The majority of the derivatives exhibited strong protective effects on PC12 (PC12 rat pheochromocytoma) cells exposed to H2O2, ... in PC12 cells. Inhibition of GCLC and HO-1 by specific inhibitors, l-buthionine-S-sulfoximine (BSO) and zinc protoporphyrin ( ... Rat PC12 cells were obtained from the Institute of Biochemistry and Cell Biology, SIBS, CAS. PC12 cells were cultured in DMEM ... Figure 1. Chalcone derivatives protected PC12 cells from H2O2-induced oxidative injury. PC12 cells were seeded in a 96-well ...
... induced neurite retraction or cell rounding in differentiated PC12 cells. The lysosphingolipid-induced shape change was ... Exogenous sphingosine 1-phosphate induces neurite retraction possibly through a cell surface receptor in PC12 cells Biochem ... induced neurite retraction or cell rounding in differentiated PC12 cells. The lysosphingolipid-induced shape change was ... require uptake of the lipid into the cells but possibly requires interaction with a cell surface receptor in the neuronal cells ...
The PC-12 cells appear to be vulnerable to cytotoxic concentrations of HNE. Experimental HNE exposure provides an intriguing ... Influence of cytotoxic doses of 4-hydroxynonenal on selected neurotransmitter receptors in PC-12 cells.. [M A Siddiqui, G Singh ... receptors was evident at 4h of exposure of PC-12 cells to HNE. The decrease in the binding with DA-D2 receptors continued till ... receptors respectively was observed at 1h exposure of PC-12 cells to HNE at 25 and 50microM concentrations. The decrease in the ...
... Exp Cell Res. 2003 ... We find that some proteins show basal levels of tyrosine nitration in PC12 cells grown in the absence of nerve growth factor ( ... The subcellular distribution of nitrated proteins changes during PC12 cell differentiation, displaying a shift from the ... and subcellular distribution of nitrated proteins during neurotrophin-induced differentiation of PC12 cells. ...
Effect of reserpine on dopamine (DA) content (A) and glutathione (GSH) content (B) of PC12 cells. PC12 cells, cultured for 2 d ... Effect of reserpine on glutathione (GSH) content of PC12 cells in the presence of cystine. PC12 cells, cultured for 2 d, were ... Cell culture. PC12 cells, originally described by Greene and Tischler (1976), were grown in 80 cm2 tissue culture flasks ... Adrenal medulla-derived cells such as the PC12 cell line are known to preferentially accumulate (radiolabeled) DA from the ...
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA)., PC-12 cell ... PC-12 cytoplasmic extract was prepared from cell cultures of the rat adrenal pheochromocytoma PC-12 cell line. ... PC-12 cytoplasmic extract was prepared from cell cultures of the rat adrenal pheochromocytoma PC-12 cell line. Extracts have ... PC-12 cell lysate, Cytoplasmic Fraction See all PC-12 cell lysate products ...
  • PC12 cells were used to find which prion protein fragments caused neuronal dysfunction. (wikipedia.org)
  • Our data show that GEN significantly inhibited Aβ25-35-induced apoptosis of PC12 cells. (rsc.org)
  • Together, these findings showed that Aβ-induced apoptosis of PC12 cells proceeds through the Fas/FasL pathway. (rsc.org)
  • Thus, our results suggest that genistein can inhibit Aβ-induced apoptosis of PC12 cells through blockage of the JNK activation and subsequent suppression of the JNK-dependent Fas/FasL pathway. (rsc.org)
  • The H2O2‑induced apoptosis of PC12 cells resulted in a reduction in the B‑cell lymphoma 2 (Bcl‑2)/Bcl‑2‑associated X protein ratio and the activation of caspase‑3. (spandidos-publications.com)
  • Nerve growth factor (NGF)-mediated activation of mitogen-activated protein kinases (MAPK) is critical for differentiation and apoptosis of PC12 cells. (ovid.com)
  • All these data suggest that the selective JNK activation by HNE is critical for the apoptosis of PC12 cells and that the HNE-mediated apoptosis is likely to be mediated through the activation of the ASK1-SEK1-JNK pathway without activation of extracellular signal-regulated kinase or p38 MAP kinase. (aspetjournals.org)
  • Conclusions: The LED irradiation, when utilized at power of 0.9 W/m 2 and 60 minutes has significantly diminished Aβ induced apoptosis of PC12 cells. (elsevier.com)
  • Conclusions: The LED irradiation, when utilized at power of 0.9 W/m2 and 60 minutes has significantly diminished Aβ induced apoptosis of PC12 cells. (elsevier.com)
  • The production of ROS and ATP in MnCl(2)-induced apoptosis of PC12 cells was examined. (bvsalud.org)
  • MnCl(2) induces apoptosis of PC12 cells , which is related to the increase of ROS, the inhibition of the mitochondria and the activation of Caspase 3 . (bvsalud.org)
  • Treatment of PC12 cells with nerve growth factor creates cells with long processes known as neurite varicosities, which contain small amounts of vesicles. (wikipedia.org)
  • Treatment of PC12 cells with dexamethasone differentiates them into chromaffin-like cells. (wikipedia.org)
  • To approach this hypothesis, caveolae were disrupted by treatment of PC12 cells with cholesterol binding drugs. (csic.es)
  • Treatment of PC12 cells with clinically applicable concentrations of L-DOPA (25-100 microM) induced cell death via a mechanism which exhibited morphological and biochemical characteristics of apoptosis, including chromatin condensation, membrane blebbing, and internucleosomal DNA fragmentation. (jci.org)
  • Studies were undertaken to compare the signal-induced redistribution of conventional protein kinase C (cPKC) to nonclassical protein kinase C (nPKC) family members in response to phorbol 12-myristate 13-acetate (PMA) or nerve growth factor (NGF) treatment of PC12 cells. (aacrjournals.org)
  • Chronic treatment of PC12 cells with PMA (1 microM) caused a rapid disappearance of alpha, beta, delta, and epsilon PKC isoforms, whereas the expression of PKC-zeta was unaltered over 4 days. (aacrjournals.org)
  • Treatment of PC12 cells with H 2 O 2 resulted in induction of apoptosis in these cells, which is accompanied by the activation of PLD. (elsevier.com)
  • Glutamate treatment of PC12 cells has been shown to result in the accumulation of intracellular inosltol phosphates suggesting the presence of glutamate metabotropic receptors (mGluRs) positively coupled to phospholipase C. The present study examined the expression of group I rnGluRs (mGluR1 and mGluR5) in PC12 cells. (elsevier.com)
  • PC12 cells treated for 10-14 days with nerve growth factor had no release of vesicles from the cell body which indicates the aggregation of vesicles in the ends of the neurites. (wikipedia.org)
  • Cells that were genetically modified to overexpress gelsolin have longer neurites and a greater neurite motility rate compared to controls. (nih.gov)
  • In response to nerve growth factor, pAntisense clones extended significantly more neurites than control cells (4.01 +/- 0.16 versus 2.93 +/- 0.16 neurites/cell). (biomedsearch.com)
  • Indirect immunofluorescence localization of these polypeptides in NGF-treated PC12 cells reveals a punctate pattern concentrated in neurites. (springer.com)
  • More than 48% of single cells produced neurites. (arvojournals.org)
  • Only 5% of single cells produced neurites. (arvojournals.org)
  • The PC12 pheochromocytoma cell line responds to nerve growth factor (NGF) by exiting from the cell cycle and differentiating to induce extending neurites. (jefferson.edu)
  • PC12 cells were seeded in poly-L-lysine-coated polycarbonate dishes and neurites were elongated by the addition of neuron growth factor. (springerprofessional.de)
  • Morphology of neurites in PC12 cells were observed by phase contrast microscopy. (springerprofessional.de)
  • In addition, swelling and beading of neurites in PC12 cells increased with increase in strain magnitudes or strain rates. (springerprofessional.de)
  • g) Withdrawal of NGF in serum-free conditions results in degeneration of neurites and loss of cell viability. (rupress.org)
  • Transport in neurites of differentiated PC12 cells was qualitatively similar to that in the somata, except that the ER stores in neurites sometimes released Ca2+ instead of clearing it after depolarization. (nih.gov)
  • Thus Mg2+-ATP, calmodulin, or proteins containing -SH or -OH groups are not necessary for exocytosis in permeabilized PC 12 cells. (uni-muenchen.de)
  • MSGs, which are also called large dense core vesicles, accumulate in cells until they undergo fusion with the plasma membrane by regulated exocytosis. (rupress.org)
  • This review attempts to touch on the history and application of amperometry at PC12 cells for fundamental investigation into the exocytosis process. (chalmers.se)
  • As will be outlined in this review, these cells have served as an extremely useful model of exocytosis in the study of the latency of stimulation-release coupling, the role of exocytotic proteins in regulation of release, effect of drugs on quantal size, autoreceptors, fusion pore biophysics, environmental factors, health and disease. (chalmers.se)
  • As PC12 cells have some advantages over other models for neurosecretion, including chromaffin cells, it is more than likely that in the following decade PC12 cells will continue to serve as a model to study exocytosis. (chalmers.se)
  • It was developed in parallel to the adrenal chromaffin cell model because of its extreme versatility for pharmacological manipulation, ease of culture, and the large amount of information on their proliferation and differentiation. (wikipedia.org)
  • The vesicles hold catecholamines, mostly dopamine but also limited amount of norepinephrine, and release of these neurotransmitters give rise to spikes due to changes in current similar to chromaffin cells. (wikipedia.org)
  • This is supposed to send the cells off toward a mature chromaffin cell morphology. (bio.net)
  • This does not require additional Mg2+-ATP (in contrast to bovine adrenal medullary chromaffin cells). (uni-muenchen.de)
  • These cells are popular because they are able to synthesize and release catecholamines in a similar manner as dopaminergic neurons and adrenal medullary chromaffin cells [ 4 ]. (biomedcentral.com)
  • We investigated the actions of rikkunshito on catecholamine release from PC12 cells, an adrenal chromaffin cell line. (biomedcentral.com)
  • PC12 cells are derived from a pheochromocytoma of the rat adrenal medulla and display the characteristics of adrenal chromaffin cells. (biomedcentral.com)
  • Our results indicate that Ca2+ transport in undifferentiated PC12 cells is quite unlike transport in adrenal chromaffin cells, for which they often are considered models.Transport in both cell states more closely resembles that of sympathetic neurons, for which differentiated PC12 cells often are considered models.Comparison with other cell types shows that different cells emphasize different Ca2+ transport mechanisms. (nih.gov)
  • Our results indicate that Ca2+ transport in undifferentiated PC12 cells is quite unlike transport in adrenal chromaffin cells, for which they often are considered models. (nih.gov)
  • Fingerprint Dive into the research topics of 'Chromaffin granule and PC12 cell chondroitin sulfate proteoglycans and their relation to chromogranin A'. Together they form a unique fingerprint. (elsevier.com)
  • Their embryological origin with neuroblastic cells means they can easily differentiate into neuron-like cells even though they are not considered adult neurons. (wikipedia.org)
  • As a first step in determining what cellular processes are regulated by the calcium-modulated protein S100A1 isoform in neurons, the effects of ablated S100A1 expression on neurite organization and microtubule/tubulin levels in PC12 cells were examined. (biomedsearch.com)
  • Our finding that L-DOPA-induced cell death in vitro occurs via apoptosis explains the lack of evidence supporting its toxicity in vivo, since apoptotic neurons are rapidly phagocytosed in vivo without causing damage to surrounding tissue. (jci.org)
  • The adaptor protein SH2B3 (Lnk) negatively regulates neurite outgrowth of PC12 cells and cortical neurons. (sigmaaldrich.com)
  • Overexpression of SH2B3 not only inhibits NGF-induced differentiation of PC12 cells but also reduces neurite outgrowth of primary cortical neurons. (sigmaaldrich.com)
  • Together, our data demonstrate that SH2B3, unlike the other two family members, inhibits neuronal differentiation of PC12 cells and primary cortical neurons. (sigmaaldrich.com)
  • Previously, it was reported that overexpression of HuD induces neuronal differentiation in PC12 cells, cortical primary culture neurons, and retinoic acid-induced teratocarcinoma cell lines ( 5 ). (asm.org)
  • These findings indicated that HuD is required for neuronal differentiation processes, including growth arrest and cell fate acquisition of neural stem/progenitor cells, and possibly for sprouting and regeneration of mature neurons. (asm.org)
  • This study characterizes the effect of electron transport chain (ETC) inhibition on mitochondrial dynamics in differentiated PC12 cells and primary cortical neurons. (tcd.ie)
  • Finally, this study demonstrated it is possible to visualize the cell surface glycome of primary cortical neurons by metabolic labelling. (tcd.ie)
  • The present results also suggest that PC12 cells have a requirement for NGF (similar to that of normal sympathetic neurons) and that serum may substitute for this requirement. (rupress.org)
  • Endogenous E2F2 increased in PC12 cells in response to nerve growth factor (NGF) and was also expressed in cerebellar granule neurons undergoing terminal differentiation. (umassmed.edu)
  • Thus, E2F2 converted PC12-derived neurons from a reversible to a 'terminally' differentiated, NGF-dependent state, analogous to postmitotic sympathetic neurons. (umassmed.edu)
  • These results indicate that E2F2 may have a unique role in maintaining the postmitotic state of terminally differentiated neurons, and may participate in apoptosis in neurons attempting to re-enter the cell cycle. (umassmed.edu)
  • Because these components possess well-defined topography, we theorize a role for topography in neuronal development and we demonstrate that survival and function of hippocampal neurons and differentiation of telencephalic neural stem cells is modulated by nanoroughness. (pnas.org)
  • Furthermore, telencephalic neural stem cells differentiated into neurons even under exogenous signals that favor astrocytic differentiation. (pnas.org)
  • Transport in both cell states more closely resembles that of sympathetic neurons, for which differentiated PC12 cells often are considered models. (nih.gov)
  • The molecular and biochemical mode of cell death of dopaminergic neurons in Parkinson's disease (PD) is uncertain. (elsevier.com)
  • Conditional genetic deletion of PTEN in CNS neurons improved neuronal survival and long-distance regeneration in both retinal ganglion cells 17 , 18 and corticospinal neurons 19 . (nature.com)
  • Our study demonstrates that in contrast to neurons from the CNS, PC12 cells do not express a normal complement of NMDA receptor-subunits, and this may be one factor limiting functional responses to NMDA/glutamate and consequently the use of PC12 cells as a neuronal model. (edu.au)
  • In addition to the monoamine (dopamine and norepinephrine) pathway, PC12 cells have been reported to express both the kynurenine and serotonin pathways. (wikipedia.org)
  • A contrary view regarding expression of the serotonin pathway by PC12 cells was expressed by the investigators who first established this cell line. (wikipedia.org)
  • This study suggests that micelle-based intracellular delivery of potent, poorly water soluble, cell-death-pathway inhibitors may represent a valuable addition to established delivery of cytocidal block-copolymer micelle-incorporated bioactives. (scientistsolutions.com)
  • Since the methylated population of HuD was reduced in NGF-treated PC12 cells, downregulation of HuD methylation is a possible pathway through which NGF induces differentiation of PC12 cells. (asm.org)
  • In the present study, we investigated the inhibitory effects of GEN on Aβ25-35-induced apoptosis in cultured PC12 cells and the related signaling pathway. (rsc.org)
  • Salidroside, extracted from the traditional Chinese herb Rhodiola rosea L, plays an essential role in reducing hypoxia-induced apoptosis in PC12 cells by the mitochondrial pathway. (medscimonit.com)
  • In this study, we found that TLR4/NF-κB pathway was activated by high glucose stimulation in PC12 and significantly alleviated by the co-treatment of ghrelin. (springer.com)
  • The potential of NGF to activate the Smad pathway independent of TGF-ß might be of special importance in regulating expression of genes that are essential for the development and function of neuronal cells or of other NGF-sensitive cells, in particular those which are TGF-ß-resistant. (uni-wuerzburg.de)
  • Nerve Growth factor regulation of cyclin D1 in PC12 cells through a p21RAS extracellular signal-regulated kinase pathway requires cooperative interactions between Sp1 and nuclear factor-kappaB. (jefferson.edu)
  • Marked activation of the JNK pathway through SEK1 and apoptosis signal-regulating kinase 1 (ASK1), an upstream kinase of SEK1, was demonstrated by the transient transfection of cDNA for wild-type SEK1 or ASK1 together with JNK into COS-7 cells. (aspetjournals.org)
  • The purpose of this study is to investigate whether local anesthetics affect the MAP kinase pathway activated by depolarization in neuronal cells. (asahq.org)
  • In this study, various aspects of the NGF intracellular signaling pathway in PC12 cells are investigated. (rupress.org)
  • A stable clone established from PC12 cells overexpressing a GFP-Peripherin fusion protein (pEGFP-Peripherin) was constructed for determining the pathway involved in neurodegeneration by biochemical, cell biology, and electronic microscopy approaches. (biomedcentral.com)
  • The findings thus indicate that certain Chinese medicines may induce neurite outgrowth by a novel mechanism which is distinct from the NGF-activated pathway in PC12 mutant cells. (elsevier.com)
  • Differentiated PC12 cells have a small component of Ca2+ clearance possessing pharmacological characteristics consistent with secretory pathway Ca2+ ATPase (SPCA), potentially residing on Golgi and/or secretory granules. (nih.gov)
  • PC12 ceils overexpressing dominant negative ras revealed that NGF-induced mGluR1 induction, but not mGluR5 expression, is dependent on ras pathway activation in these cells. (elsevier.com)
  • According to the results of cell metabolomics, the vital cell transduction pathway related to the crucial cell metabolic pathways were selected. (researchsquare.com)
  • Considering the antioxidant properties, this research focuses on protective effects of peel and seed extracts of C. aurantium against glutamate toxicity in PC12 cells. (termedia.pl)
  • The mechanism of MPP + -induced toxicity in PC12 cells was analyzed based on their genes functions, biological process, pathways and previous published literatures. (biomedcentral.com)
  • Both linoleic acid and arachidonic acid are able to inhibit MPP + -induced toxicity in PC12 cells. (biomedcentral.com)
  • In order to find the effects of these fruits, neuron like PC12 cells were exposed to the extracts of these fruits before H(2)O(2) treatment. (nih.gov)
  • Exploiting this capacity of SCBD, we challenged mechanosensing/-transduction and differentiative behavior of neuron-like PC12 cells with diverse nanotopographies and/or changes of their biomechanical status, and analyzed their phosphoproteomic profiles in these settings. (frontiersin.org)
  • SOD2 Mediates Amifostine-Induced Protection against Glutamate in PC12 Cells," Oxidative Medicine and Cellular Longevity , vol. 2016, Article ID 4202437, 11 pages, 2016. (hindawi.com)
  • Replacement by chloride of glutamate, which could sustain mitochondrial ATP production in permeabilized cells, does not significantly alter catecholamine release induced by Ca2+. (uni-muenchen.de)
  • Rajabian A, Sadeghnia H, Moradzadeh M, Hosseini A. Rheum turkestanicum reduces glutamate toxicity in PC12 and N2a cell lines. (termedia.pl)
  • In this study, we investigated the neuroprotective effects of Rheum turkestanicum in the glutamate-induced rat pheochromocytoma (PC12 cells) and mouse neuroblastoma (N2a) cell lines. (termedia.pl)
  • All these findings indicated that R. turkestanicum protected PC12 and N2a cells against glutamate-induced oxidative cell death and apoptosis and might raise the possibility of R. turkestanicum usage as a neuroprotective agent. (termedia.pl)
  • PC12 and neuro 2a cells have different susceptibilities to acetylcholinesterase-amyloid complexes, amyloid 25-35 fragment, glutamate, and hydrogen peroxide. (termedia.pl)
  • Hosseini A, Sadeghnia H, Rajabian A. Protective effects of peel and seed extracts of Citrus aurantium on glutamate-induced cytotoxicity in PC12 cell line. (termedia.pl)
  • This cell model is also widely applied to study cellular glutamate toxicity [38]. (termedia.pl)
  • One actin accessory protein, gelsolin, is involved with remodeling the cytoskeleton, although its role in cell motility is unclear. (nih.gov)
  • The 14-3-3 protein is a family of acidic, dimeric proteins distributed widely among eukaryotic cells. (springer.com)
  • This protein family binds to a variety of proteins in a phosphorylation-dependent manner and participates in the regulation of cell proliferation, differentiation and function. (springer.com)
  • We previously showed, using PC12nnr5 cells transfected with myc-tagged 14-3-3β and η) isoforms, that 14-3-3s form a complex with tyrosine hydroxylase (TH) particularly when the hydroxylase is phosphorylated at Ser-19 by endogeneous Ca 2+ , calmodulin-dependent protein kinase II 1 . (springer.com)
  • In this study we have employed proteomic techniques for systematic characterization of PC12 proteins and identified the TH protein as a major constituent of the proteins that are co-immunoprecipitated with 14-3-3. (springer.com)
  • 04 +/- 0.5 ng of alpha-tubulin/mg of total protein) when compared with control cells. (biomedsearch.com)
  • Thus, Htt and mHtt-mediated functional changes of VDAC, being predominantly VDAC1, which occur shortly after these protein appearances in cells, may result in differences concerning mitochondria functioning and viability of cells expressing Htt and mHtt. (frontiersin.org)
  • SH2B adaptor protein family members (SH2B1-3) regulate various physiological responses through affecting signaling, gene expression, and cell adhesion. (sigmaaldrich.com)
  • Overall protein kinase C activities in E1A-expressing PC12 cells were decreased, but the activity of membrane-bound protein kinase C was significantly increased. (asm.org)
  • Comparison of the effects of NGF, activators of protein kinase C and a calcium ionophore on the expression of Thy-1 and N-CAM in PC12 cell cultures. (springer.com)
  • We characterized a large protein complex containing a nerve-growth-factor-activated ERK (extracellular-signal-regulated kinase) and MEK (MAPK/ERK kinase) in rat pheochromocytoma (PC12) cells. (biochemj.org)
  • We compared the role of tyrosine kinases in α 1A -adrenergic receptor (AR) and growth factor receptor stimulation of mitogen-activated protein kinase pathways in PC12 cells. (biochemj.org)
  • These studies show that activation of mitogen-activated protein kinase pathways by G-protein-coupled receptors and tyrosine kinase receptors proceed through distinct molecular pathways in PC12 cells, and support an obligatory role for Src activation in mitogenic responses to α 1A -ARs in these cells. (biochemj.org)
  • Multiple pathways were suggested to be involved in the mechanism of MPP + -induced toxicity, including oxidative stress, DNA and protein damage, cell cycling arrest, and apoptosis. (biomedcentral.com)
  • A role for zeta protein kinase C in nerve growth factor-induced differentiation of PC12 cells -- Wooten et al. (aacrjournals.org)
  • Accumulations of this protein have been shown to inhibit complex I. Overexpression of both wild-type and mutant forms of a-synculein were found to decrease mitochondrial fusion in differentiated PC12 cells, suggesting that a-synculein dependent changes in fusion/fission dynamics may be a complicating factor in PD. (tcd.ie)
  • NGF-induction of the metalloproteinase-transin/stromelysin in PC12 cells: involvement of multiple protein kinases. (rupress.org)
  • While the GRE-containing binding sites were more often located nearby genes involved in general cell functions and processes such as apoptosis, cell motion, protein dimerization activity and vasculature development, the binding sites without a GRE were located nearby genes with a clear role in neuronal processes such as neuron projection morphogenesis, neuron projection regeneration, synaptic transmission and catecholamine biosynthetic process. (biomedcentral.com)
  • Norepinephrine and dopamine secretion into the culture medium of PC12 cells were also dose-dependently augmented by rikkunshito and/or forskolin, but experiments with a protein kinase C (PKC) activator and a phosphodiesterase inhibitor revealed that the effects of rikkunshito were not simply due to the modulation of PKC or phosphodiesterase activity. (biomedcentral.com)
  • Both stimuli activate growth-associated protein 43 (GAP-43) indicating that PC12 cells undergo substantial neuronal differentiation. (biomedcentral.com)
  • The Schwann cell conditioned medium contains a protein factor (greater than 12 kDa and broken down by trypsin treatment) with remarkable thermal stability (unaffected at 95 degrees C for 15 min) and the ability to bind heparin. (ox.ac.uk)
  • Epidermal and nerve growth factors (EGF and NGF) activate protein synthesis and initiation factor eIF2B in rat phaeochromocytoma (PC12) cells. (sahmriresearch.org)
  • Using western blot technique we observed that there is no change in NCS-1 and DARPP-32 protein levels in both PC12 WT and PC12 Clone cells after typical and atypical antipsychotic treatments. (biomedcentral.com)
  • Undifferentiated PC12 cells were found to express both mGluR5 mRNA and receptor protein by reverse transcription polymerase chain reaction (RT-PCR) and western blot techniques. (elsevier.com)
  • Nerve growth factor-induced differentiation of PC12 cells resulted in the induction of rnGluR1α and mGluR1β mRNA and mGluR1α protein. (elsevier.com)
  • Nerve growth factor (NGF) induced differentiation of PC12 cells, and after 12 days of NGF treatment there were large increases in the levels of the heterotrimeric G protein subunits αo1, αi1, β, and αs, small increases in those of αi2 and αq, and a slight decrease in that of αo2. (elsevier.com)
  • Addition of NGF to cultured PC12 cells produced increases in mRNAs corresponding to the 68 kd neurofilament protein (NF68) and the Thy-1 glycoprotein within 24 h, with maximal effects of some 90- and 45-fold stimulation (relative to beta-actin mRNA) being observed after 12 and 4 days of treatment, respectively. (royalholloway.ac.uk)
  • We examined NR1 splice variant and NR2 subunit expression in four PC12 cell-lines (ATCC, WEHI, Ordway and Flinders), correlated mRNA expression with protein expression, and used patch-clamp recordings to test functionality. (edu.au)
  • However, agents known to prevent neuronal programmed cell death (cyclic AMP, KCl, aurintricarboxylic acid, and cycloheximide) failed to prevent the 25-OH-cholesterol-mediated cytotoxicity. (biomedsearch.com)
  • These results suggest that (-)-sesamin protects 6-OHDA-induced cytotoxicity via the activation of transient ERK1/2-BadSer112 system and the inhibition of sustained ERK-p38MAPK-JNK1/2-caspase-3 system in PC12 cells. (ovid.com)
  • A series of celastrol analogues were synthesized, and their neuroprotective effect against t-BHP-induced cytotoxicity was investigated in neuronal PC12 cells. (semanticscholar.org)
  • In this study, we investigated the effects of various magnitudes and rates of strain on cytotoxicity, mortality and morphology of Rat pheochromocytoma cell line, PC12 cells using the shock machine. (springerprofessional.de)
  • After exposure to impact, lactate dehydrogenase (LDH) was measured as cytotoxicity and cell viability was measured by the dye exclusion method with trypan blue dye as cell mortality. (springerprofessional.de)
  • As a result, cytotoxicity and cell mortality increased with increase in strain magnitude and strain rate. (springerprofessional.de)
  • In the current study, the potential of two omega-6 fatty acids (i.e. arachidonic acid and linoleic acid) in alleviating 1-methyl-4-phenylpyridinium (MPP + )-induced cytotoxicity in PC12 cells was examined. (biomedcentral.com)
  • However, more recently it has become clear that cellular Ca2+ overload, or perturbation of intracellular Ca2+ compartmentalization, can cause cytotoxicity and trigger either apoptotic or necrotic cell death. (scienceopen.com)
  • Shyamala Mruthinti, Nicholas Capito, Ajay Sood and Jerry J. Buccafusco, " Cytotoxicity of Aβ 1-42, RAGE23-54, and An Aβ -RAGE Complex in PC-12 Cells", Current Alzheimer Research (2007) 4: 581. (eurekaselect.com)
  • abstract = "PC12 cells are an established model for studying the role of N-methyl-d-aspartate (NMDA) receptors in excitotoxicity and function as multimeric assemblies of NR1 with at least one NR2(A-D) subunit. (edu.au)
  • It was found that PC12 cells persist and continue to proliferate within the brain of SD rats whereas it has previously been reported that grafts placed into peripheral sites do not proliferate. (deepdyve.com)
  • However, since NCS-1 and DARPP-32 levels are not altered in wild type rats, we treated wild type PC12 cells (PC12 WT) and PC12 cells stably overexpressing NCS-1 (PC12 Clone) with antipsychotics to investigate if NCS-1 upregulation modulates DARPP-32 expression in response to antipsychotics treatment. (biomedcentral.com)
  • Neuroglobin protects PC12 cells against oxidative stress. (nih.gov)
  • To examine the anti-oxidative role of neuroglobin, PC12 cells were transfected with wild type and mutant (H64 V/H96A) Ngb for 48 h and then treated with H2O2 (0.1, 0.2 and 0.4 mM) for 6 h. (nih.gov)
  • Their results showed that rAAV/ABAD-DP-6His increased superoxide dismutase activity in hydrogen peroxide-induced oxidative stress-mediated injury of PC12 cells. (eurekalert.org)
  • Overall, the experimental findings, published in the Neural Regeneration Research , indicate that rAAV/ABAD-DP-6His generates the fusion peptide, Aβ-ABAD decoy peptide, which effectively protects PC12 cells from oxidative stress injury induced by hydrogen peroxide, thus exerting neuroprotective effects. (eurekalert.org)
  • We found a significant viability of PC12 cells by the MTT reduction test, which indicated that the phenolics of banana, orange, and apple fruits prevented oxidative stress-induced neurotoxicity. (nih.gov)
  • Additional tests by lactate dehydrogenase and trypan blue exclusion assays showed that the extracts reduced oxidative stress-induced neuronal cell membrane damage. (nih.gov)
  • These results suggest that fresh apples, banana, and orange in our daily diet along with other fruits may protect neuron cells against oxidative stress-induced neurotoxicity and may play an important role in reducing the risk of neurodegenerative disorders such as Alzheimer's disease. (nih.gov)
  • These data suggest that GSH is involved in the granular storage of DA in PC12 cells and that, considering the molecular characteristics of the granular transport system, it is likely that GSH is used to protect susceptible parts of this system against (possibly DA-induced) oxidative damage. (jneurosci.org)
  • Based on these data, we proposed that the degeneration of the nigro-striatal system in PD may be the outcome of a self-sustaining cycle that is initiated by lowering of the capacity to scavenge the H 2 O 2 released during normal DA metabolism, leading to a decrease in the granular storage and enhanced breakdown of DA, inducing more oxidative stress, disturbance of neuronal function and, eventually, cell death. (jneurosci.org)
  • This work aims to ascertain the structure-activity relationship of FA and its alkyl esters (FAEs) for evaluating the antioxidant activities in PC12 cells and Aβ 1-42 aggregation inhibitory activities in vitro , as well as the signaling mechanisms against oxidative stress elicited by Aβ 1-42 in PC12 cells. (rsc.org)
  • We report here that in an in vitro model for oxidative stress, H2O2 treatment, overexpression of TorsinA was protective against cell death. (fu-berlin.de)
  • he intracellular distribution of 125I-labeled nerve growth factor (NGF) in rat pheochromocytoma PC12 cells was studied by quantitative electron microscopic (EM) autoradiography and by subcellular fractionation. (jneurosci.org)
  • Pre-treating cells with butyl ferulate (FAC4) significantly attenuated Aβ 1-42 -evoked intracellular ROS formation. (rsc.org)
  • Real-time intracellular calcium level recordings were performed on PC-12 cell suspensions, and ET-1 induced a dose-dependent decrease in the K + -evoked calcium levels. (aspetjournals.org)
  • The cell viability, lactate dehydrogenase (LDH) activity, apoptosis, mitochondrial membrane potential (MMP), intracellular Ca2+, caspase-3 activity, and expression of Bcl-2 were detected in PC12 cells after the hypoxia treatment. (medscimonit.com)
  • To expound the protective mechanism, we investigated the effects of ghrelin on high glucose-induced cell apoptosis and intracellular signaling in cultured PC12, which is a suitable model for studying neuronal cell death. (springer.com)
  • The actions of rikkunshito on PC12 cells were evaluated by measuring intracellular cAMP levels, tyrosine hydroxylase (TH) and vasoactive intestinal peptide (VIP) mRNA expression levels, and catecholamine levels in the culture medium. (biomedcentral.com)
  • Major sub-categories include cellular growth and differentiation, cell migration, chemotaxis, cell adhesion, small molecule biochemistry aiming at changing intracellular concentrations of second messengers such as Ca2+ and cAMP as well as expression of enzymes involved in posttranslational modification of proteins. (biomedcentral.com)
  • We previously demonstrated that cells which have spread on ECM release more dopamine and contain less intracellular dopamine than cells which are rounded on plastic cultureware. (elsevier.com)
  • Membrane composition serves to identify intracellular compartments, signal cell death, as well as to alter a cell's electrical and physical properties. (chalmers.se)
  • Cell survival in both untreated and nerve growth factor (NGF)-treated PC12 cells was inhibited by down-regulation of SOD1, with NGF-treated cells dying at lower levels of inhibition than untreated cells. (pnas.org)
  • These results suggest that the induction of cell death by inhibition of SOD1 is due to free radical induction of apoptosis and that growth factor therapy for free-radical-mediated disease may require antioxidants in order to be effective. (pnas.org)
  • On the other hand, antisense-mediated knockdown of HuD resulted in the inhibition of neurite extension in PC12 cells ( 48 ) and HuD-deficient mice exhibited a larger population of dividing stem cells in the adult subventricular zone ( 3 ). (asm.org)
  • Preincubation of PC12 cells with pertussis toxin reversed the ET-1-induced inhibition of the K + -evoked ATP release. (aspetjournals.org)
  • Nongenomic inhibition of catecholamine secretion by 17beta-estradiol in PC12 cells. (semanticscholar.org)
  • Inhibition of any of the ETC complexes, ATP synthase or dissipation of the mitochondrial membrane potential was sufficient to completely abolish mitochondrial fusion in differentiated PC12 cells. (tcd.ie)
  • In this study, inhibition of fatty acid oxidation and pyruvate transport into the mitochondrion decreased mitochondrial fusion in differentiated PC12 cells. (tcd.ie)
  • A decrease in the level of O-GlcNAcylation, induced by inhibition or knockdown of O-GlcNAc transferase, resulted in increased mitochondrial fusion, decreased mitochondrial membrane potential, fragmented mitochondrial morphology and decreased respiration rates in differentiated PC12 cells. (tcd.ie)
  • We used pharmacological inhibition of Ca2+ transporters to characterize Ca2+ clearance after depolarizations in both undifferentiated and nerve growth factor-differentiated PC12 cells. (nih.gov)
  • The inhibition of cell growth was not dependent on an inhibition of complex I activity since MPP + also inhibited cell proliferation in SH-SY5Y cells lacking mitochondrial DNA and complex I activity (p 0 cells). (elsevier.com)
  • Li, X & Jope, RS 1995, ' Selective Inhibition of the Expression of Signal Transduction Proteins by Lithium in Nerve Growth Factor‐Differentiated PC12 Cells ', Journal of neurochemistry , vol. 65, no. 6, pp. 2500-2508. (elsevier.com)
  • PC12 is a cell line derived from a pheochromocytoma of the rat adrenal medulla, that have an embryonic origin from the neural crest that has a mixture of neuroblastic cells and eosinophilic cells. (wikipedia.org)
  • Nerve growth factor (NGF) induces survival and differentiation of the neural crest-derived PC12 cell line. (csic.es)
  • We observe the morphology of PC12 cells using the multi photon microscope and evaluate effectiveness of uniform ACMF stimulation of the nerve axonal extension and the neural network generation. (asme.org)
  • At physiological concentrations, forms of Aβ have different, but relatively low potencies as cytotoxicants in neural cells in culture. (eurekaselect.com)
  • PC12 cells have been widely used as a model for neural differentiation and as such they have been used to examine the effects of differentiation on exocytotic release and specifically release at varicosities. (chalmers.se)
  • During continuous culture of neural PC12 cells, we obtained a drug-hypersensitive PC12 mutant cell that showed high stimulation of neurite outgrowth by various drugs. (elsevier.com)
  • Our results provide clear evidence that Schwann cells produce factors other than those already known to stimulate a neural phenotype in PC12 cells, and which thus have potential regeneration enhancing effects. (ox.ac.uk)
  • Here we employed the CRISPR/dCas9 system to repress PTEN transcription in neural cells. (nature.com)
  • dCas9-KRAB delivered in a lentiviral vector with one CRISPR guide RNA (gRNA) achieved potent and specific PTEN repression in human cell line models and neural cells derived from human iPSCs, and induced histone (H)3 methylation and deacetylation at the PTEN promoter. (nature.com)
  • The CRISPR system also worked more effectively than shRNAs for Pten repression in rat neural crest-derived PC-12 cells, and enhanced neurite outgrowth after nerve growth factor stimulation. (nature.com)
  • Since NGF employs stress-activated c-Jun N-terminal kinase (JNK) to regulate both programmed cell death and neurite outgrowth of PC12 cells, we examined NGF-regulated JNK activity and the role of Gi/o proteins. (ovid.com)
  • Hypoxic/ischemic brain injury is associated with accumulation of reactive oxygen species (ROS) and/or reactive nitrogen species (RNS), and antioxidants or ROS scavengers promote cell survival. (nih.gov)
  • Using PC12 cell model, we showed that Glu and Hcy provoked a huge amount of reactive oxygen species (ROS) production, causing mitochondrial damage at EC 50 20 and 10 mm, respectively. (mdpi.com)
  • Furthermore, PF significantly mitigated the H2O2‑induced reduction in cell viability, in addition to scavenging reactive oxygen species and preventing the release of lactate dehydrogenase from the PC12 cells. (spandidos-publications.com)
  • To explore the mechanism of reactive oxygen species (ROS) in manganese chloride (MnCl(2))-induced apoptosis in PC12 cells . (bvsalud.org)
  • Cell viability was assessed by MTT assay. (nih.gov)
  • Cell viability following transfection of Ngb siRNA and treatment of H 2 O 2 . (nih.gov)
  • Cell viability and apoptotic ratio were assessed using the MTT and Annexin V staining tests, respectively. (hindawi.com)
  • In preconditioned cells, we found that cell viability remained high following exposure to 6-OHDA (150 μ M). OA treatment enhanced the protective effects of preconditioning. (hindawi.com)
  • Because after 48 h of Htt and mHtt expression differences in mitochondria functioning co-occurred with differences in the cell viability, we decided to estimate the effect of Htt and mHtt expression lasted for 48 h on VDAC functioning. (frontiersin.org)
  • Cell viability was measured using an MTT assay as described in a previous study ( 9 ). (spandidos-publications.com)
  • Cell viability was then assessed by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. (biomedcentral.com)
  • Cells treated with 500 μM MPP + for a day reduced cell viability to ~70% as compared to control group. (biomedcentral.com)
  • Incubation of differentiated PC-12 cells with 10-100 μM Aβ1-42 or with RAGE23-54 resulted in concentration-dependent decreases in cell viability. (eurekaselect.com)
  • PC12 is a clonal line of rat pheochromocytoma (7). (springer.com)
  • Howard B.D., Melega W.P. (1981) Acetylcholine Metabolism in PC12, A Clonal Cell Line on Secretory Cells. (springer.com)
  • PC12 cells, a rat clonal pheochromocytoma cell line [ 29 ], possess dopamine synthesis, metabolism and transporter systems [ 30 - 32 ], and therefore have been used as a model for studies of MPP + neurotoxicity and PD. (biomedcentral.com)
  • Rat pheochromocytoma clonal cell line. (ch3biosystems.com)
  • The present study investigated the effects of PF on hydrogen peroxide (H2O2)‑induced apoptosis in PC12 cells, to improve the understanding of the mechanisms underlying its neuroprotective properties. (spandidos-publications.com)
  • In this study, we examined the role of PLD activity in hydrogen peroxide (H 2 O 2 )-induced apoptosis in rat pheochromocytoma (PC12) cells. (elsevier.com)
  • In this study, we examined the role of PLD activity in hydrogen peroxide (H2O2)-induced apoptosis in rat pheochromocytoma (PC12) cells. (elsevier.com)
  • PC12 cell line use has given much information to the function of proteins underlying vesicle fusion. (wikipedia.org)
  • To date, more than 70 proteins, localized in the cell membrane to nuclei, are reported to bind with the 14-3-3 family. (springer.com)
  • Ichimura T., Itagaki C., Wakamiya A., Isobe T. (2002) Interaction of Tyrosine Hydroxylase and 14-3-3 Proteins in PC12 Cells. (springer.com)
  • Quantitative RT-PCR (qRT-PCR) and western blot analysis showed that 1 and 1d significantly improve the expression of nuclear factor erythroid 2 p45-related factor 2 (Nrf2)-dependent antioxidant genes g-Glutamylcysteine Ligase Catalytic Subunit ( GCLC ) and heme oxygenase-1 ( HO-1 ) and their corresponding proteins (γ-glutamyl cysteine synthase (γ-GCS) and HO-1) in PC12 cells. (mdpi.com)
  • Adenovirus E1A proteins stimulate inositol phospholipid metabolism in PC12 cells. (asm.org)
  • Hu proteins have been identified as target antigens in the sera of patients with paraneoplastic encephalomyelitis, an autoimmune disease associated with small-cell lung cancer and neuroblastoma ( 20 , 64 ). (asm.org)
  • PC12 cells express juvenile microtubule-associated proteins during nerve growth factor-induced neurite outgrowth. (springer.com)
  • These lipid peroxides can directly damage cells and tissues through interaction with cellular macromolecules, including proteins, lipids, and nucleic acids. (aspetjournals.org)
  • To test any influence of proNGF on pure NGF expression fingerprinting, the expression level of a set of candidate genes was analysed by qReal-Time PCR in rat adrenal pheochromocytoma cell line PC12, treated with a mixture of NGF and proNGF recombinant proteins, in different stoichiometric ratios. (biomedcentral.com)
  • This initial characterization of the transcriptional signature of proNGF in PC12 cells confirmed that the mature and the precursor NGF proteins are biologically different, showing a different transcriptional signature. (biomedcentral.com)
  • Because we observed no alteration in NCS-1 and DARPP-32 levels in both PC12 WT and Clone cells treated with typical or atypical antipsychotics, we suggest that the alteration in levels of both proteins in schizophrenic's PFC is related to psychopathology but not with antipsychotic treatment. (biomedcentral.com)
  • This investigation examined if lithium, the primary therapeutic treatment for bipolar affective disorder, modulated the levels of selected signal transduction proteins in PC12 cells. (elsevier.com)
  • In summary, use of the dynamic system of NGF-induced PC12 cell differentiation provided a sensitive model in which to identify signal transduction proteins that were influenced by lithium treatment. (elsevier.com)
  • These results suggested that stretch-induced injury of PC12 cells is dependent on strain magnitude and strain rate, but, in order to elucidate neuronal damage caused by an interaction between strain magnitude and strain rate, more quantitative studies are required, such as various combination of strain magnitude and strain rate. (springerprofessional.de)
  • Gelsolin overexpression enhances neurite outgrowth in PC12 cells. (nih.gov)
  • Cyclin D1 induction by NGF governs differentiation and neurite outgrowth in PC12 cells. (jefferson.edu)
  • Glucocorticoids increase dopamine production in PC12 cells presumably via an increase in tyrosine hydroxylase gene transcription. (elsevier.com)
  • Bethea, CL 1987, ' Glucocorticoid stimulation of dopamine production in PC12 cells on extracellular matrix and plastic ', Molecular and Cellular Endocrinology , vol. 50, no. 3, pp. 211-222. (elsevier.com)
  • In summary, ablation of S100A1 expression in PC12 cells results in increased tubulin levels, altered neurite organization, and decreased cell growth. (biomedsearch.com)
  • The means of the averaged log ratio of the paired samples were used to identify the fold changes of gene expression in PC12 cells after MPP + treatment. (biomedcentral.com)
  • Protective effect of rAAV/ABAD-DP on H2O2-mediated changes in morphology of PC12 cells. (eurekalert.org)
  • Cells treated with this agent showed condensed nuclei, a morphology similar to that of cells dying of programmed cell death. (biomedsearch.com)
  • This chapter presents a model for neuronal function, differentiation, and survival of cultured pheochromocytoma PC12 cell lines. (elsevier.com)
  • The nerve growth factor induced differentiation of a pheochromocytoma PC12 cell line. (wizdom.ai)
  • Dispersed and aggregated PC12 cell grafts contained immunoreactivity to tyrosine hydroxylase and aromatic amino acid decarboxylase, but failed to express phenylethanolamine n-methyltransferase. (deepdyve.com)
  • Furthermore, co-treatment with (-)-sesamin (30 mg/kg, p.o.) once a day for 28 days significantly increased the number of tyrosine hydroxylase-immunopositive neuronal cells and the levels of dopamine, norepinephrine, 3,4-dihydroxyphenylacetic acid, and homovanillic acid in the substantia nigra-striatum of 6-OHDA-lesioned rat model of PD with or without L-DOPA treatment. (ovid.com)
  • Here, we show a novel mechanism of arginine methylation of HuD by coactivator-associated arginine methyltransferase 1 (CARM1) that affected mRNA turnover of p21 cip1/waf1 mRNA in PC12 cells. (asm.org)
  • These results suggested that CARM1-methylated HuD maintains PC12 cells in the proliferative state by committing p21 cip1/waf1 mRNA to its decay system. (asm.org)
  • Given that HuD-bound gene products are involved in cell cycle arrest (p21 cip1/waf1 and p27), neurite outgrowth (GAP43 and tau), and functional differentiation (choline acetyltransferase) ( 21 ), HuD is presumed to induce the neuronal cell shape by exerting a protective effect on these ARE-containing labile mRNAs by antagonizing ARE-mediated mRNA decay. (asm.org)
  • In previous work, we found that nerve growth factor (NGF) induced expression of the mRNA transcript encoding the metalloproteinase transin/stromelysin in PC12 cells. (rupress.org)
  • However, mGtuR1 mRNA was not detected in these cells and western blot analysis showed only faint mGluR1α immunoreactivity suggesting a very low level of rnGluR1 expression. (elsevier.com)
  • Real-time PCR showed variable levels of NR1 mRNA expression (all splice variants) between cell-lines and a significant increase in response to nerve growth factor in the WEHI and Ordway lines (NGF: 50 ng/ml, 2.1- and 13.4-fold increases, respectively, P ≤ 0.05). (edu.au)
  • mRNA for NR2A or NR2B was not detected in any PC12 cell-line. (edu.au)
  • Thus, S100A1 may directly link the cytoskeleton and calcium signal transduction pathways to cell proliferation. (biomedsearch.com)
  • Neurotoxicity of 25-OH-cholesterol on NGF-differentiated PC12 cells. (biomedsearch.com)
  • PC12 cells induced to differentiate with nerve growth factor were used to study the neurotoxicity of 25-OH-cholesterol. (biomedsearch.com)
  • Experimental HNE exposure provides an intriguing model of toxicant-cell interactions involving neurotransmitter receptors in HNE neurotoxicity. (sigmaaldrich.com)
  • The present study investigated the effects of (-)-sesamin on 6-hydroxydopamine (6-OHDA)-induced neurotoxicity using PC12 cells and dopaminergic neuronal cells of 6-OHDA-lesioned rat model of Parkinson's disease (PD). (ovid.com)
  • Is there any method i can use to dissolve the lipid to induce the pc12 cells? (scientistsolutions.com)
  • During neuronal differentiation, cell-substrate interaction is sufficient to induce directional migration along the nanogratings. (lancs.ac.uk)
  • Nerve growth factor and epidermal growth factor induce rapid transient changes in proto-oncogene transcription in PC12 cells. (springer.com)
  • Stable cell lines, expressing variable amounts of optimedin, were treated with nerve growth factor (NGF) to induce neuronal differentiation. (arvojournals.org)
  • NGF and proNGF induce distinct transcriptional signatures in target cells, highlighting their different bioactivities. (biomedcentral.com)
  • Cells can sense nanotopographical cues deriving from the extracellular matrix (ECM), predominantly through integrin adhesion complexes (IAC), and the microenvironmental information is converted into alterations of the cytoskeletal organization and mechanics. (frontiersin.org)
  • The optimedin-expressing cells were more strongly attached to collagen I or collagen IV extracellular matrix compared with control cells. (arvojournals.org)
  • After the PC12 cells were stimulated by acetylcholine, only the extracellular Ca 2+ concentration increased while there was no increase in the extracellular K + concentration. (go.jp)
  • In PC12 cells, treatment with (-)-sesamin (25 μM) reduced 6-OHDA (100 μM)-induced cell death and induced transient extracellular signal-regulated kinase (ERK1/2) phosphorylation and Bad phosphorylation at Ser112 (BadSer112). (ovid.com)
  • PC12 cells are spherical when cultured on plastic, but flatten and spread extensively when cultured on extracellular matrix (ECM) produced by bovine corneal endothelial cells. (elsevier.com)
  • However, the CNS is also composed of extracellular matrix macromolecules and glia support cells, and the contribution of the physical attributes of these components in maintenance and regulation of neuronal function is not well understood. (pnas.org)
  • d) NGD also induces neurite outgrowth from PC12 cells in serum-free medium. (rupress.org)
  • These properties make it possible to compare the metabolism and granular storage of two different transmitters produced by the same secretory cell. (springer.com)
  • To study the influence of nuclear oncogenes on inositol phospholipid metabolism, we examined the various parameters of inositol phospholipid metabolism in PC12 cells expressing adenovirus type 12 or adenovirus type 5 E1A. (asm.org)
  • These observations clearly indicate that inositol phospholipid metabolism is stimulated in cells producing E1A and suggest that nuclear oncogene E1A has the ability to stimulate inositol phospholipid metabolism. (asm.org)
  • Mitochondrial Ca2+ homeostasis plays a key role in the regulation of aerobic metabolism and cell survival 1 , but the molecular identity of the Ca2+ channel, the mitochondrial calcium uniporter 2 , was still unknown. (scienceopen.com)
  • Overexpression of wild type Ngb, but not of mutant Ngb, significantly attenuated H2O2-induced ROS/RNS accumulation and lipid peroxidation, decreased H2O2-induced mitochondrial dysfunction and apoptosis, and promoted overall cell survival. (nih.gov)
  • Wüllner, U. 1999-04-02 00:00:00 We investigated the effect of glutathione (GSH) depletion on mitochondrial function and generation of reactive oxygen intermediates (ROI) in PC12 cells in vitro. (deepdyve.com)
  • In contrast, BSO-treated cells still maintained 100% GSH in the mitochondrial fraction for 4 h and 6% for 48 h. (deepdyve.com)
  • Background: T-type Ca2+ (Cav3.2) channel inhibitors prevent neuronal death during ischemia, but the cytotoxic signals have not been resolved.Results: Cav3.2 channels generate cytotoxic Ca2+ elevations in the cytosol and mitochondria of PC12 cells deprived of oxygen and glucose.Conclusion: Ca2+ transfer from Cav3.2 channels to mitochondria contributes to ischemic toxicity.Significance: Cav3.2 channels and the mitochondrial Ca2+ uniporter (MCU) are potential targets for the treatment of stroke. (scienceopen.com)
  • siRNA silencing of MCU in HeLa cells drastically reduced mitochondrial Ca2+ uptake. (scienceopen.com)
  • Complex I of the mitochondrial electron transport chain was markedly inhibited in IH exposed cells. (elsevier.com)
  • The ATP pool in differentiated cells was much more labile than that of undifferentiated cells in the presence of agents that dissipated mitochondrial proton gradients. (nih.gov)
  • S100A1 regulates neurite organization, tubulin levels, and proliferation in PC12 cells. (biomedsearch.com)
  • In contrast, SH2B3 was reported to inhibit cell proliferation during the development of immune system. (sigmaaldrich.com)
  • E2Fs play a central role in cell proliferation and growth arrest through their ability to regulate genes involved in cell cycle progression, arrest and apoptosis. (umassmed.edu)
  • Treatment of undifferentiated PC12 cells with MPP + primarily inhibited proliferation of PC12 cells and secondarily led to cell death after the depletion of all energy substrates by glycolysis. (elsevier.com)
  • Permeabilization with α-toxin or digitonin and stimulation of the cells were done consecutively to wash out endogenous Mg2+-ATP. (uni-muenchen.de)
  • In this study, we design and fabricate a new 3D bio-reactor, which can implement uniform AC magnetic field (ACMF) stimulation on PC12 cells. (asme.org)
  • Sakiyama R, Matsumoto K, Yamamoto K, Morita Y, Nakamachi E. Development of AC Magnetic Field Stimulation Bio-Reactor for Three-Dimensional Culture of PC12 Cells. (asme.org)
  • The design of materials to promote the development and/or regeneration of neuronal tissue requires the understanding of the mechanisms by which the underlying substrate topography can modulate neuronal cell differentiation and migration. (lancs.ac.uk)
  • 3,4 MAP kinase is highly expressed in the central nervous system, primarily in such postsynaptic structures as neuronal cell bodies and the bases of the proximal dendrites. (asahq.org)
  • In addition to this, use of inhibitors of various glycosyltransferases allowed for some elucidation of the make-up of the neuronal cell surface glycome which was found to be predominantly N-glycans. (tcd.ie)
  • Therefore, we applied inducible PC12 cell model of HD to determine the relationship between the effect of expression of wild type and mutant huntingtin (Htt and mHtt, respectively) on cell survival and mitochondria functioning in intact cells under conditions of undergoing cell divisions. (frontiersin.org)
  • Pretreatment of PC12 cells with a survival-promoting agent, 8-(4-chlorophenylthio)-cAMP, prevented both the HNE-induced JNK activation and apoptosis. (aspetjournals.org)
  • f) The apparent minimum level of 2.5S NGF required for PC12 survival and morphological differentiation in serum-free medium is about 10 ng/ml (approximately 0.4 nM). (rupress.org)
  • It has long been known that Ca2+ signals govern a host of vital cell functions and so are necessary for cell survival. (scienceopen.com)
  • Greene, Lloyd A. / Cultured PC12 cells : A model for neuronal function, differentiation, and survival . (elsevier.com)
  • Effects of Schwann cell secreted factors on PC12 cell neuritogenesis and survival. (ox.ac.uk)
  • We have used PC12 cells to examine the effects of factors secreted by Schwann cells that promote cell survival and neurite outgrowth, and hence are likely candidates for promoting neuronal regeneration. (ox.ac.uk)
  • Schwann cell conditioned media also enhanced PC12 cell survival. (ox.ac.uk)
  • This contrasts with the effects of E2F4, which enhances the differentiation state of PC12 cells without affecting cell cycle parameters or survival. (umassmed.edu)
  • Furthermore, transfection with TorsinA significantly increased survival of PC12 after H2O2 treatment. (fu-berlin.de)
  • Cell apoptosis was confirmed by morphological criteria, DNA fragmentation assay, and FAScan flow cytometer assay. (elsevier.com)
  • This cell death showed no morphological characteristics of apoptosis and was not blocked by treatment with caspase inhibitors. (elsevier.com)
  • These gene activation events were independent of overt morphological differentiation of PC12 cells occurring both under conditions permissive and non-permissive for neurite outgrowth, and once established the new molecular phenotype was dependent upon the continued presence of NGF. (royalholloway.ac.uk)
  • Norepinephrine (NE) (noradrenaline), epidermal growth factor (EGF) and nerve growth factor (NGF) caused different patterns of tyrosine phosphorylation in PC12 cells stably expressing α 1A -ARs. (biochemj.org)
  • We investigated the effects of 17beta-estradiol, an estrogen, on [(3)H]norepinephrine ([(3)H]NE) secretion in PC12 cells. (semanticscholar.org)
  • We now report that rikkunshito stimulates the release of norepinephrine and dopamine from PC12 cells via a novel mechanism involving cAMP. (biomedcentral.com)
  • PC12 cells stop dividing and terminally differentiate when treated with nerve growth factor or dexamethasone. (wikipedia.org)
  • I intended to differentiate PC12 cell with NGF. (bio.net)
  • In response to nerve growth factor (NGF) induction, PC12 cells differentiate into a sympathetic neuron-like phenotype. (biomedcentral.com)
  • Changes in the gene expression pattern of control versus optimedin in PC 12 cells were assessed using rat Affymetrix arrays. (arvojournals.org)
  • In this study, Agilent rat oligo 22K microarrays were used to examine alterations in gene expression of PC12 cells after 500 μM MPP + treatment. (biomedcentral.com)
  • Relative gene expression of control and treated cells represented by spot intensities on the array chips was analyzed using bioinformatics tools. (biomedcentral.com)
  • SIGNALS initiated by neurotransmitter release are transferred to the nuclei of the postsynaptic cells to activate specific programs of gene expression, thus influencing synaptic functions. (asahq.org)
  • Rikkunshito dose-dependently enhanced forskolin-induced elevations in cAMP in PC12 cells, and also increased the gene expression of TH and VIP. (biomedcentral.com)
  • We found that, when PC12 cells are treated with proNGF/NGF mixtures, a unique pattern of gene expression, which does not overlap with that deriving from treatment with either proNGF or NGF alone, is induced. (biomedcentral.com)
  • We have previously characterized the distinct properties of proNGF and NGF signalling by gene expression microarray in PC12 cells and identified two subsets of differentially expressed genes that could be ascribed to a "pure proNGF" and a "pure NGF" signalling, respectively [ 8 ]. (biomedcentral.com)
  • Rat pheochromocytoma PC12 cells were stably transfected with a tetracycline-inducible vector containing optimedin cDNAs. (arvojournals.org)
  • In contrast, pretreatment of PC12 cells with these drugs inhibited the NGF- but not the epidermal growth factor-lnduced MAPK phosphorylation without affecting the TrkA autophosphorylation. (csic.es)
  • However, pretreatment with the root extract of R. turkestanicum significantly reduced MDA content, ROS generation and apoptotic cell death. (termedia.pl)
  • Pretreatment of PC12 cells with SB203580, a specific inhibitor of p38 MAP kinase, had no effect on HNE-induced apoptosis. (aspetjournals.org)
  • This cell line has been used to understand the role of synaptotagmin in vesicle-cell membrane fusion. (wikipedia.org)
  • Taken together, our results demonstrate the presence of caveolae in PC12 ceils, which contain the high affinity NGF receptor TrkA, and the specific involvement of these cholesterol-enriched plasma membrane microdomains in the propagation of the NGF-induced signal. (csic.es)
  • Therefore, we isolated VDAC from the cells and tested the preparations by black lipid membrane system. (frontiersin.org)
  • EM autoradiography showed that 125I-NGF was localized at the plasma membrane and cytoplasmic compartments but did not accumulate in the nuclear chromatin or in the nuclear membrane compartment of cells analyzed after 1 hr and 1, 2, and 8 d of incubation with 125I-NGF. (jneurosci.org)
  • Diacylglycerol kinase activity in the membrane fraction was 10 to 15% of that in parental PC12 cells. (asm.org)
  • In neuroendocrine PC12 cells, immature secretory granules (ISGs) mature through homotypic fusion and membrane remodeling. (rupress.org)
  • Effects of selective estrogen receptor modulators on plasma membrane estrogen receptors and catecholamine synthesis and secretion in cultured bovine adrenal medullary cells. (semanticscholar.org)
  • In this study, we suppressed ganglioside biosynthesis in PC12 cells with the glucosylceramide synthase inhibitor, N-butyldeoxynojirimycin and observed reduced plasma membrane antibody binding and a major neuroprotective effect in complement-mediated lysis assays. (gla.ac.uk)
  • Sarco-endoplasmic reticulum Ca2+ ATPase (SERCA), plasma membrane Ca2+ ATPase (PMCA), and Na+/Ca2+ exchanger (NCX) account for almost all Ca2+ clearance in both cell states, with NCX and PMCA making the greatest contributions. (nih.gov)
  • Other organics have been studied using this cell line to understand their effects on PC12 cells These types of studies show that use of PC12 cell line can be a model for past and future neurotoxicological studies. (wikipedia.org)
  • In addition, dexamethasone-differentiated cells have been shown to have an increased number of releasable vesicles with increased quantal size, thereby allowing for an even broader range of applications including neuropharmacological and neurotoxicological studies. (chalmers.se)
  • In this study, we demonstrate that L-DOPA induces death of catecholaminergic cells in vitro via an active program of apoptosis. (jci.org)
  • These results suggest PC12 cells may be a useful model for investigating the regulation and expression of group I rnGluR isoforms and their role in neuronal processes in vitro. (elsevier.com)