Pasteurellaceae: A family of coccoid to rod-shaped nonsporeforming, gram-negative, nonmotile, facultatively anaerobic bacteria that includes the genera ACTINOBACILLUS; HAEMOPHILUS; MANNHEIMIA; and PASTEURELLA.Pasteurellaceae Infections: Infections with bacteria of the family PASTEURELLACEAE.Actinobacillus: A genus of PASTEURELLACEAE described as gram-negative, nonsporeforming, nonmotile, facultative anaerobes. Most members are found both as pathogens and commensal organisms in the respiratory, alimentary, and genital tracts of animals.Pasteurella: The oldest recognized genus of the family PASTEURELLACEAE. It consists of several species. Its organisms occur most frequently as coccobacillus or rod-shaped and are gram-negative, nonmotile, facultative anaerobes. Species of this genus are found in both animals and humans.Mannheimia: Genus of bacteria in the family PASTEURELLACEAE, comprising multiple species that do not ferment trehalose. Species include MANNHEIMIA HAEMOLYTICA; M. glucosida, M. granulomatis, M. ruminalis, and M. varigena.Haemophilus: A genus of PASTEURELLACEAE that consists of several species occurring in animals and humans. Its organisms are described as gram-negative, facultatively anaerobic, coccobacillus or rod-shaped, and nonmotile.Pasteurella Infections: Infections with bacteria of the genus PASTEURELLA.Pasteurella pneumotropica: A species of gram-negative bacteria in the genus PASTEURELLA, found in the NASOPHARYNX of normal GUINEA PIGS; RATS; HAMSTERS; MICE; DOGS; and CATS. When associated with disease, it is usually a secondary invader. Occasional infections have been reported in humans.Moraxellaceae: A family of gram-negative, aerobic bacteria in the order Pseudomonadales. Some strains are parasites of the mucosal membranes of animals and humans; others are found in association with food products or in the environment.Porpoises: Mammals of the family Phocoenidae comprising four genera found in the North Pacific Ocean and both sides of the North Atlantic Ocean and in various other seas. They differ from DOLPHINS in that porpoises have a blunt snout and a rather stocky body while dolphins have a beak-like snout and a slender, streamlined body. They usually travel in small groups. (From Walker's Mammals of the World, 5th ed, pp1003-4)Haemophilus somnus: A species of gram-negative bacteria (currently incertae sedis) causing multisystem disease in CATTLE.Pasteurella multocida: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria normally found in the flora of the mouth and respiratory tract of animals and birds. It causes shipping fever (see PASTEURELLOSIS, PNEUMONIC); HEMORRHAGIC BACTEREMIA; and intestinal disease in animals. In humans, disease usually arises from a wound infection following a bite or scratch from domesticated animals.Haemophilus paragallinarum: A species of gram-negative bacteria in the genus HAEMOPHILUS, causing respiratory tract disease in CHICKENS known as infectious coryza.Mannheimia haemolytica: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria normally commensal in the flora of CATTLE and SHEEP. But under conditions of physical or PHYSIOLOGICAL STRESS, it can cause MASTITIS in sheep and SHIPPING FEVER or ENZOOTIC CALF PNEUMONIA in cattle. Its former name was Pasteurella haemolytica.RNA, Ribosomal, 16S: Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.Vibrionaceae: A family of gram-negative bacteria whose members predominate in the bacterial flora of PLANKTON; FISHES; and SEAWATER. Some members are important pathogens for humans and animals.Actinobacillus pleuropneumoniae: A species of gram-negative, facultatively anaerobic coccobacillus-shaped bacteria that has been isolated from pneumonic lesions and blood. It produces pneumonia with accompanying fibrinous pleuritis in swine.Haemophilus ducreyi: A species of HAEMOPHILUS that appears to be the pathogen or causative agent of the sexually transmitted disease, CHANCROID.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.DNA, Ribosomal: DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Actinobacillus Infections: Infections with bacteria of the genus ACTINOBACILLUS.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Genes, rRNA: Genes, found in both prokaryotes and eukaryotes, which are transcribed to produce the RNA which is incorporated into RIBOSOMES. Prokaryotic rRNA genes are usually found in OPERONS dispersed throughout the GENOME, whereas eukaryotic rRNA genes are clustered, multicistronic transcriptional units.

Phocoenobacter uteri gen. nov., sp. nov., a new member of the family Pasteurellaceae Pohl (1979) 1981 isolated from a harbour porpoise (Phocoena phocoena). (1/146)

Phenotypic and phylogenetic studies were performed on a Gram-negative, rod-shaped bacterium isolated from the uterus of a porpoise. Biochemical and physiological studies indicated that the bacterium was related to the family Pasteurellaceae. Comparative 16S rRNA gene sequencing studies confirmed these findings and demonstrated that the bacterium represents a hitherto unknown subline within this family of organisms. Based on the results of the phylogenetic analysis and phenotypic criteria, it is proposed that the bacterium be assigned to a new genus, Phocoenobacter uteri gen. nov., sp. nov. The type strain of Phocoenobacter uteri sp. nov. is NCTC 12872T.  (+info)

DNA-DNA hybridization determined in micro-wells using covalent attachment of DNA. (2/146)

The present study was aimed at reducing the time and labour used to perform DNA-DNA hybridizations for classification of bacteria at the species level. A micro-well-format DNA hybridization method was developed and validated. DNA extractions were performed by a small-scale method and DNA was sheared mechanically into fragments of between 400 and 700 bases. The hybridization conditions were calibrated according to DNA similarities obtained by the spectrophotometric method using strains within the family Pasteurellaceae. Optimal conditions were obtained with 300 ng DNA added per well and bound by covalent attachment to NucleoLink. Hybridization was performed with 500 ng DNA, 5% (w/w) of which was labelled with photo-activatable biotin (competitive hybridization) for 2.5 h at 65 degrees C in 2 x SSC followed by stringent washing with 2 x SSC at the same temperature. The criteria for acceptance of results were a maximum of 15% standard deviation, calculated as a percentage of the mean for four replicate micro-wells, and that DNA similarities were not significantly different in at least two independent experiments. The relationship between DNA similarities obtained by the micro-well method (y) and by the spectrophotometric method (x) was y = 0.534x+30.6, when these criteria had been applied to 23 pairs of strains of Actinobacillus species, avian [Pasteurella] haemolytica-like bacteria and Mannheimia species. The correlation (Pearson) between DNA similarities obtained by interchange of strains used for covalent binding and hybridization was 0.794. Significantly lower DNA similarities were observed by the spectrophotometric compared with the micro-well method for three pairs of hybridizations. After removal of these data, the relationship between DNA similarities obtained by the micro-well and spectrophotometric methods improved to y = 0.855x + 11.0. It was found that the accuracy and precision of the micro-well method was at the same level as that of the spectrophotometric method, but the labour and analysis time were reduced significantly. The use of hybridization in the micro-well format will allow DNA-DNA hybridizations to be carried out between all strains selected for a particular taxonomic study, in order to construct complete data matrices and improve species definition.  (+info)

Seasonal incidence and antibiotic susceptibility patterns of Pasteurellaceae isolated from American bison (Bison bison). (3/146)

Ninety pharyngeal tonsils were collected from 2-year-old American bison (Bison bison) bulls and sampled for members of the Pasteurellaceae family. Particular attention was paid to seasonal incidence and antimicrobial resistance in serotypes and biovariants. Multiple strains of Pasteurella haemolytica (39%), P. trehalosi (68%), P. multocida (34%) and Haemophilus somnus (13%) were cultured from 86 out of the 90 (96%) tonsil samples. Pasteurella trehalosi was the most common and evenly distributed of the organisms recovered. Pasteurella haemolytica was found in fewer numbers than P. trehalosi, but showed an increase in number of isolates recovered with each sampling period. Pasteurella multocida, both A and D capsular types, was recovered from all sampling periods. No serotype pattern was observed in any of the animal groups sampled. One hundred twenty-seven of 147 (86%) of the isolates were resistant to at least 1 antibiotic, 95/147 (65%) to at least 2 different antibiotics, and 16/147 (11%) to at least 3 antibiotics. The most common resistance pattern observed was to neomycin and spectinomycin (73/147) (49%).  (+info)

Characterization of Haemophilus ducreyi-specific T-cell lines from lesions of experimentally infected human subjects. (4/146)

Haemophilus ducreyi is the etiologic agent of chancroid, a sexually transmitted genital ulcer disease that facilitates the transmission of human immunodeficiency virus. In the human model of infection, the histopathology of infected sites in part resembles a delayed-type hypersensitivity (DTH) response. In this study, T cells were isolated from skin biopsy specimens obtained from 24 subjects who were infected for 7 to 14 days. One clone and 12 lines that responded to H. ducreyi antigens were obtained from 12 of the subjects. Fluorescence-activated cell sorter analysis showed that the antigen-responsive lines and clone were predominantly CD3(+) and CD4(+). The lines and clone responded to H. ducreyi antigen in a dose-dependent manner and produced gamma interferon (IFN-gamma) alone or IFN-gamma and interleukin-10 (IL-10) but no IL-4 or IL-5 in response to H. ducreyi. Proliferation of T cells was dependent on the presence of autologous antigen-presenting cells. The lines showed little response to antigens prepared from other members of the Pasteurellaceae and responded to different fractions of H. ducreyi separated by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. We conclude that T cells that recognize H. ducreyi antigens are recruited to sites experimentally infected with the organism. The lack of cross-reactivity to the Pasteurellaceae and the response of the lines to different antigen fractions suggest that subjects are sensitized to H. ducreyi during the course of infection.  (+info)

Antimicrobial resistance in pasteurella and mannheimia: epidemiology and genetic basis. (5/146)

Isolates of the genera Pasteurella and Mannheimia cause a wide variety of diseases of great economic importance in poultry, pigs, cattle and rabbits. Antimicrobial agents represent the most powerful tools to control such infections. However, increasing rates of antimicrobial resistance may dramatically reduce the efficacy of the antimicrobial agents used to control Pasteurella and Mannheimia infections. This review presents a short summary of the infections caused by Pasteurella and Mannheimia isolates in food-producing animals and the possibilities of preventing and controlling primary and secondary pasteurellosis. Particular reference is given to antimicrobial chemotherapy and the resistance properties of Pasterurella and Mannheimia isolates. The genetic basis of the most predominant resistance properties such as resistance to beta-lactam antibiotics, tetracyclines, aminoglycosides, sulfonamides, and chloramphenicol is discussed. This is depicted with reference to the role of plasmids and transposons in the spread of the resistance genes among Pasteurellaceae and members of other bacterial families and genera.  (+info)

Tetracycline resistance genes in isolates of Pasteurella multocida, Mannheimia haemolytica, Mannheimia glucosida and Mannheimia varigena from bovine and swine respiratory disease: intergeneric spread of the tet(H) plasmid pMHT1. (6/146)

Tetracycline-resistant isolates of Pasteurella multocida and Mannheimia spp. from respiratory diseases in cattle and swine were investigated for the classes of tet gene and their chromosomal or plasmid location. The 34 isolates comprised eight P. multocida, 23 Mannheimia haemolytica, two Mannheimia varigena and a single Mannheimia glucosida isolate. Identification of the tet genes was achieved by PCR analysis and hybridization with specific probes. Transformation and hybridization experiments served to confirm the plasmid location of tet genes. Selected tet genes and their adjacent regions were sequenced. The tet genes tet(B), tet(G) and tet(H) were detected. The gene tet(H) was present in 26 isolates. The 4.4 kb tet(H)-carrying plasmid pMHT1 was detected in six isolates representing all four species. In the remaining 28 isolates, copies of tet(B), tet(G) and tet(H) were identified as chromosomal. No correlation between the tet gene type and the MIC of tetracycline, or between the number of tet gene copies and the MIC of tetracycline was observed. Tetracycline resistance in P. multocida and Mannheimia spp. is mediated by at least three different tet genes. A new type of tet(H)- carrying plasmid, pMHT1, was identified. The detection of pMHT1 in M. glucosida and M. varigena is the first report of resistance plasmids in isolates of these two species. For the first time, tet(G) genes were detected in members of the family Pasteurellaceae.  (+info)

Nucleotide sequence and organization of plasmid pMVSCS1 from Mannheimia varigena: identification of a multiresistance gene cluster. (7/146)

OBJECTIVES: A small resistance plasmid of Mannheimia varigena was analysed with regard to its gene organization and the development of a multiresistance gene cluster. MATERIALS AND METHODS: The 5621 bp plasmid pMVSCS1 was transformed into Escherichia coli JM107, cloned and sequenced completely. RESULTS: Three intact resistance genes, sulII, catAIII and strA, a truncated strB gene and a novel replication gene were identified. A potential recombination site for the integration of catAIII in the spacer region between sulII and strA was identified. CONCLUSION: The physical linkage of the plasmid-borne resistance genes organized in a cluster would facilitate the spread of the different resistance genes by co-selection, even in the absence of a direct selective pressure.  (+info)

Genetic diversity of Gallibacterium anatis isolates from different chicken flocks. (8/146)

Amplified fragment length polymorphisms (AFLPs) were used to characterize the genotypic diversity of a total of 114 Gallibacterium anatis isolates originating from a reference collection representing 15 biovars from four countries and isolates obtained from tracheal and cloacal swab samples of chickens from an organic, egg-producing flock and a layer parent flock. A subset of strains was also characterized by pulsed-field gel electrophoresis and biotyping. The organic flock isolates were characterized by more than 94% genetic similarity, indicating that only a single clone was apparent in the flock. The layer parent flock isolates were grouped into two subclusters, each with similarity above 90%. One subcluster contained only tracheal isolates, while the other primarily included cloacal isolates. In conclusion, we show that AFLP analysis enables fingerprinting of G. anatis, which seems to have a clonal population structure within natural populations. There was further evidence of clonal lineages, which may have adapted to different sites within the same animal.  (+info)

*Pasteurella canis

Hansen, M.J.; Bertelsen, M.F.; Christensen, H.; Bisgaard, M.; Bojesen, A.M. (2012). "Occurrence of Pasteurellaceae bacteria in ... Pasteurella canis is a Gram-negative, nonmotile, penicillin-sensitive coccobacillus belonging to the Pasteurellaceae family. ...

*Actinobacillus pleuropneumoniae

Members of the Pasteurellaceae family routinely change the cellular processes of the infected cell. In particular, A. ... A. pleuropneumoniae is a nonmotile, Gram-negative, encapsulated coccobacillus bacterium found in the Pasteurellaceae family. It ...

*Pasteurellaceae

The Pasteurellaceae comprise a large family of Gram-negative bacteria. Most members live as commensals on mucosal surfaces of ... Pasteurellaceae are typically rod-shaped, and are a notable group of facultative anaerobes. Their biochemical characteristics ... Comparative analyses of Pasteurellaceae genomes have identified large numbers (>20) of conserved signature indels (CSIs) in ... Based upon many other CSIs that are specific for subgroups of Pasteurellaceae species, it has been proposed to divide the ...

*International Committee on Systematics of Prokaryotes

Family Pasteurellaceae; Photosynthetic prokaryotes; Pseudomonas, Xanthomonas and related organisms; Suborder Pseudonocardineae ...

*Pasteurellosis

Hemorrhagic septicemia Pasteurellaceae Kuhnert P; Christensen H, eds. (2008). Pasteurellaceae: Biology, Genomics and Molecular ...

*Actinobacillus

It is a member of the Pasteurellaceae family. The bacteria are facultatively aerobic or anaerobic, capable of fermenting ... 2008). "Actinobacillus pleuropneumoniae". Pasteurellaceae: Biology, Genomics and Molecular Aspects. Caister Academic Press. ... ISBN 978-1-904455-34-9. Kuhnert P; Christensen H (editors). (2008). Pasteurellaceae: Biology, Genomics and Molecular Aspects. ...

*Mannheimia

... is a Gram-negative, anaerobe, non-spore-forming and non-motile bacteria genus from the family of Pasteurellaceae. ... ISBN 1-4051-5823-9. Kuhnert, [edited by] Peter; Christensen, Henrik (2008). Pasteurellaceae : biology, genomics and molecular ...

*Haemophilus

2008). Pasteurellaceae: Biology, Genomics and Molecular Aspects. Caister Academic Press. ISBN 978-1-904455-34-9. [1]. Tortora(1 ... coccobacilli bacteria belonging to the Pasteurellaceae family. While Haemophilus bacteria are typically small coccobacilli, ...

*Pasteurella

2008). Pasteurellaceae: Biology, Genomics and Molecular Aspects. Caister Academic Press. ISBN 978-1-904455-34-9. Health ...

*Pasteurella multocida

... is a Gram-negative, nonmotile, penicillin-sensitive coccobacillus belonging to the Pasteurellaceae family ... Kuhnert P; Christensen H (editors). (2008). Pasteurellaceae: Biology, Genomics and Molecular Aspects. Caister Academic Press. ...

*Haemophilus influenzae

2008). Pasteurellaceae: Biology, Genomics and Molecular Aspects. Caister Academic Press. ISBN 978-1-904455-34-9. [1] About the ... Pasteurellaceae Maurice Hilleman Hattie Alexander Hib vaccine Haemophilus influenzae cellulitis Haemophilus meningitis Trimeric ... facultatively anaerobic pathogenic bacterium belonging to the Pasteurellaceae family. H. influenzae was first described in 1892 ...

*Haemophilus felis

... is a Gram-negative species of bacterium belonging to the Pasteurellaceae family. A strain of this species was ... Korczak, B. (1 July 2004). "Phylogeny of the family Pasteurellaceae based on rpoB sequences". International Journal of ...

*RTX toxin

Members of the Pasteurellaceae family also produce RTX toxins. The Vibrio genus, which includes V. cholerae and V. vulnificus, ... Frey J (November 2011). "The role of RTX toxins in host specificity of animal pathogenic Pasteurellaceae". Veterinary ...

*Uptake signal sequence

... are short DNA sequences preferentially taken up by competent bacteria belonging to the Pasteurellaceae family (e.g., ... "Evolutionary stability of DNA uptake signal sequences in the Pasteurellaceae". Proceedings of the National Academy of Sciences ...

*Avibacterium

... is a Gram-positive genus from the family of Pasteurellaceae. Parte, A.C. "Avibacterium". www.bacterio.net. " ...

*Actinobacillus suis

... is a beta-haemolytic, Gram-negative bacterium of the Pasteurellaceae family. The bacterium has many strains ...

*Aggregatibacter iron-regulated sRNA

JA 03 and JA04 are most widely conserved and have orthologues across many Pasteurellaceae. HrrF RNA is another Fur-regulated ...

*Haemophilus pittmaniae

... is a Gram-negative species of bacterium belonging to the Pasteurellaceae family. Strains of this species ...

*Pasteurella stomatis

... , is a Gram-negative, nonmotile, penicillin-sensitive coccobacillus belonging to the Pasteurellaceae family ...

*Pasteurella testudinis

... is a Gram-negative, nonmotile, rod-shaped species of bacteria belonging to the Pasteurellaceae family. ...

*Pasteurella anatis

... penicillin-sensitive coccobacillus belonging to the Pasteurellaceae family. Bacteria from this family cause zoonotic infections ...

*List of MeSH codes (C01)

... pasteurellaceae infections MeSH C01.252.400.700.030 --- actinobacillus infections MeSH C01.252.400.700.030.178 --- ...

*Natural competence

Another complicating factor is the self-bias of the DNA uptake systems of species in the family Pasteurellaceae and the genus ... but bacteria in the families Neisseriaceae and Pasteurellaceae preferentially take up DNA fragments containing short DNA ...

*List of MeSH codes (B03)

Pasteurellaceae MeSH B03.440.450.600.450 --- Haemophilus MeSH B03.440.450.600.450.125 --- Haemophilus ducreyi MeSH B03.440. ... pasteurellaceae MeSH B03.660.250.550.050 --- Actinobacillus MeSH B03.660.250.550.050.050 --- Actinobacillus ...
The Pasteurellaceae comprise a large family of Gram-negative bacteria. Most members live as commensals on mucosal surfaces of birds and mammals, especially in the upper respiratory tract. Pasteurellaceae are typically rod-shaped, and are a notable group of facultative anaerobes. Their biochemical characteristics can be distinguished from the related Enterobacteriaceae by the presence of oxidase, and from most other similar bacteria by the absence of flagella. Bacteria in the family Pasteurellaceae have been classified into a number of genera based on metabolic properties, but these classifications are not generally accurate reflections of the evolutionary relationships between different species. Haemophilus influenzae was the first organism to have its genome sequenced and has been studied intensively by genetic and molecular methodologies. The Haemophilus genus is a notorious human pathogen associated with bacteremia, pneumonia, meningitis and chancroid. Other pathogenic members belonging to ...
Pasteurellaceae é uma família de bactérias gram-negativas da ordem Pasteurellales e do filo Proteobacteria. Actinobacillus Haemophilus Lonepinella Pasteurella Mannheimia Phocoenobacter GARRITY, G.M.; BELL, J.A.; LILBURN, T.: Order XIV. Pasteurellales ord. nov. In: D.J. BRENNER, N.R. KRIEG, J.T. STALEY and G. M. GARRITY (eds), Bergeys Manual of Systematic Bacteriology, second edition, vol. 2 (The Proteobacteria), part B (The Gammaproteobacteria), Springer, New York, 2005, p. 850. Christensen H; Bisgaard M (2008). "Taxonomy and biodiversity of members of Pasteurellaceae". Pasteurellaceae: Biology, Genomics and Molecular Aspects. Caister Academic Press ...
A domestic hen showing infraorbital swelling was presented for a routine classroom demonstration of avian diagnostics. At necropsy, tiny whitish caseo-calcareous nodules were found in the subcutaneous tissues of the carcass, produced by the subcutaneous fowl mite, Laminosioptes cysticola. Gallibacterium anatis biovar haemolytica was isolated from the infraorbital sinus, it containing a caseous exudate. During necropsy, a conspicuous cyst was found in the abdominal cavity. Microscopic examination of the internal lining of the cyst revealed a single cuboidal to columnar, ciliated epithelium, leading to a diagnosis of oviductal cyst. Also, the microscopic examination of the heart, lung, liver and kidney reveal a multifocal infiltration of lymphoma cells. It appears the first case of simultaneous presentation of these conditions from a single chicken ...
Histophilus somni is a Gram-negative bacterium and member of the Pasteurellaceae that is responsible for respiratory disease and other systemic infections in cattle. One of the bacteriums virulence factors is antigenic phase variation of its lipooligosaccharide (LOS). LOS antigenic variation may occur through variation in composition or structure of glycoses or their substitutions, such as phosphorylcholine (ChoP). However, the role of ChoP in the pathogenesis of H. somni disease has not been established. In Haemophilus influenzae ChoP on the LOS binds to platelet activating factor on epithelial cells, promoting bacterial colonization of the host upper respiratory tract. However, ChoP is not expressed in the blood as it also binds C-reactive protein, resulting in complement activation and killing of the bacteria. In order to simulate the susceptibility of calves with suppressed immunity due to stress or previous infection, calves were challenged with bovine herpes virus-1 or dexamethazone 3 days prior
Histophilus somni is a Gram-negative bacterium and member of the Pasteurellaceae that is responsible for respiratory disease and other systemic infections in cattle. One of the bacteriums virulence factors is antigenic phase variation of its lipooligosaccharide (LOS). LOS antigenic variation may occur through variation in composition or structure of glycoses or their substitutions, such as phosphorylcholine (ChoP). However, the role of ChoP in the pathogenesis of H. somni disease has not been established. In Haemophilus influenzae ChoP on the LOS binds to platelet activating factor on epithelial cells, promoting bacterial colonization of the host upper respiratory tract. However, ChoP is not expressed in the blood as it also binds C-reactive protein, resulting in complement activation and killing of the bacteria. In order to simulate the susceptibility of calves with suppressed immunity due to stress or previous infection, calves were challenged with bovine herpes virus-1 or dexamethazone 3 ...
Effective publication: POHL (S.): Reklassifizierung der gattung Actinobacillus Brumpt 1910, Haemophilus Winslow et al. 1971 und Pasteurella Trevisan 1887 anhand phänotypischer und molekularer daten, insbesondere der DNS-verwandtschaften bei DNS: DNS-hybridisierung in vitro und vorschlag einer neuen familie, Pasteurellaceae. Inaug. Diss. Phillips-Universität Marburg/Lahn. Mauersberger, Marburg, 1979 ...
We investigated three bovine respiratory pathobionts in healthy cattle using qPCR optimised and validated to quantify Histophilus somni, Mannheimia haemolytica and Pasteurella multocida over a wide dynamic range. A longitudinal study was conducted to investigate the carriage and density of these bacteria in the nasal passages of healthy beef calves (N = 60) housed over winter in an experimental farm setting. The three pathobiont species exhibited remarkably different carriage rates and density profiles. At housing, high carriage rates were observed for P. multocida (95%), and H. somni (75%), while fewer calves were positive for M. haemolytica (13%). Carriage rates for all three bacterial species declined over the 75-day study, but not all individuals became colonised despite sharing of environment and airspace. Colonisation patterns ranged from continuous to intermittent and were different among pathobiont species. Interval-censored exponential survival models estimated the median duration of H. somni
A. actinomycetemcomitans is an important periodontopathogen that has been implicated in juvenile and adult periodontitis, diseases characterized by rapid destruction of the tooth-supporting tissues. This organism possesses a large number of virulence factors which enable it to colonize the oral cavity, invade periodontal tissues, evade host defences, initiate connective tissue destruction and interfere with tissue repair (for recent reviews see [59, 60]).. Most fresh isolates of A. actinomycetemcomitans are fimbriated, and binding of this microorganism to solid surfaces (e.g. hydroxyapatite and saliva-coated hydroxyapatite) may involve fimbriae [61]. The parameters of A. actinomycetemcomitans adhesion to epithelial cells were assayed by use of different cell lines including the KB cell line, derived from a human oral epidermoid carcinoma [62-64]. Adhesion of A. actinomycetemcomitans to epithelial cells involves multiple determinants (fimbriae, OMPs, vesicles, and/or an extracellular amorphous ...
Lineage: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Aggregatibacter; Aggregatibacter ...
Lineage: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Mannheimia; Mannheimia ...
welcome to the emaa 2011 Bienvenue à l emaa 2011 welcome to the emaa 2011 Bienvenue à l emaa 2011 Dear Colleague, For its 7th year, the EMAA (European Masters in Aesthetic and Anti-Aging Medicine) will
This paper describes a phenotypic and genotypic investigation of the taxonomy of [Haemophilus] paragallinarum, Pasteurella gallinarum, Pasteurella avium and Pasteurella volantium, a major subcluster within the avian 16S rRNA cluster 18 of the family Pasteurellaceae. An extended phenotypic characterization was performed of the type strain of [Haemophilus] paragallinarum, which is NAD-dependent, and eight NAD-independent strains of [Haemophilus] paragallinarum. Complete 16S rRNA gene sequences were obtained for one NAD-independent and four NAD-dependent [Haemophilus] paragallinarum strains. These five sequences along with existing 16S rRNA gene sequences for 11 other taxa within avian 16S rRNA cluster 18 as well as seven other taxa from the Pasteurellaceae were subjected to phylogenetic analysis. The analysis demonstrated that [Haemophilus] paragallinarum, Pasteurella gallinarum, Pasteurella avium and Pasteurella volantium formed a monophyletic group with a minimum of 96·8 % sequence similarity. This
Aggregatibacter actinomycetemcomitans is a Gram-negative pathogen that is the etiologic agent of localized aggressive periodontitis (LAP) and other systemic inf...
Aggregatibacter actinomycetemcomitans ATCC ® 700685D-5™ Designation: Genomic DNA from Aggregatibacter actinomycetemcomitans strain HK1651 TypeStrain=False Application:
Aggregatibacter actinomycetemcomitans ATCC ® 700685D-5™ Designation: Genomic DNA from Aggregatibacter actinomycetemcomitans strain HK1651 TypeStrain=False Application:
Background Aggregatibacter actinomycetemcomitans is genetically heterogeneous and comprises distinct clonal lineages that may have different virulence potentials. However, limited information of the strain-to-strain genomic variations is available. Methodology/Principal Findings The genome sequences of 11 A. actinomycetemcomitans strains (serotypes a-f) were generated de novo, annotated and combined with three previously sequenced genomes (serotypes a-c) for comparative genomic analysis. Two major groups were identified; serotypes a, d, e, and f, and serotypes b and c. A serotype e strain was found to be distinct from both groups. The size of the pangenome was 3,301 genes, which included 2,034 core genes and 1,267 flexible genes. The number of core genes is estimated to stabilize at 2,060, while the size of the pangenome is estimated to increase by 16 genes with every additional strain sequenced in the future. Within each strain 16.7-29.4% of the genome belonged to the flexible gene pool. Between any
Adolescent; Aggregatibacter actinomycetemcomitans; Bacterial Toxins; Cohort Studies; Cross-Sectional Studies; DNA, Bacterial; Disease Progression; Ghana; Humans; Pasteurellaceae Infections; Periodontal Diseases ...
Aggregatibacter actinomycetemcomitans is a Gram-negative organism, strongly associated with aggressive forms of periodontitis. An important virulence property of A. actinomycetemcomitans is its ability to form tenacious biofilms that can attach to abiotic as well as biotic surfaces. The histone-like (H-NS) family of nucleoid-structuring proteins act as transcriptional silencers in many Gram-negative bacteria. To evaluate the role of H-NS in A. actinomycetemcomitans, hns mutant derivatives of serotype a strain D7S were generated. Characteristics of the hns mutant phenotype included shorter and fewer pili, and substantially lower monospecies biofilm formation relative to the wild type. Furthermore, the D7S hns mutant exhibited significantly reduced growth within a seven-species oral biofilm model. However, no apparent difference was observed regarding the numbers and proportions of the remaining six species regardless of being co-cultivated with D7S hnsor its parental strain. Proteomics analysis ...
Aim We investigated the association between angiographically verified coronary artery disease (CAD) and subgingival Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola. Materials and Methods The cross-sectional study population (n = 445) comprised 171 (38.4%) patients with Stable CAD, 158 (35.5%) with acute coronary syndrome (ACS) and 116 (26.1%) with no significant CAD (No CAD). All patients participated in clinical and radiological oral health examinations. Pooled subgingival bacterial samples were analysed by checkerboard DNA-DNA hybridization assays. Results In all study groups, the presence of P. gingivalis, T. forsythia and T. denticola indicated a significant (p ≤ 0.001) linear association with the extent of alveolar bone loss (ABL), but A. actinomycetemcomitans did not (p = 0.074). With a threshold level of bacterial cells 1 × 105 A. actinomycetemcomitans was significantly more prevalent in the Stable CAD group (42.1%) compared ...
The detection of bacterial-specific volatile metabolites may be a valuable tool to predict infection. Here we applied a real-time mass spectrometric technique to investigate differences in volatile metabolic profiles of oral bacteria that cause periodontitis. We coupled a secondary electrospray ionization (SESI) source to a commercial high-resolution mass spectrometer to interrogate the headspace from bacterial cultures and human saliva. We identified 120 potential markers characteristic for periodontal pathogens Aggregatibacter actinomycetemcomitans (n = 13), Porphyromonas gingivalis (n = 70), Tanerella forsythia (n = 30) and Treponema denticola (n = 7) in in vitro cultures. In a second proof-of-principle phase, we found 18 (P. gingivalis, T. forsythia and T. denticola) of the 120 in vitro compounds in the saliva from a periodontitis patient with confirmed infection with P. gingivalis, T. forsythia and T. denticola with enhanced ion intensity compared to two healthy controls. In conclusion, ...
The ability to selectively target specific biofilm species with antimicrobials would enable control over biofilm consortium composition, with medical applications in treatment of infections on mucosal surfaces that are colonized by a mixture of beneficial and pathogenic microorganisms. We functionalized a genetically engineered multimeric protein with both a targeting moiety (biotin) and either a fluorophore or a photosensitizer (SnCe6). Biofilm microcolonies of Aggregatibacter actinomycetemcomitans, a periodontal pathogen, were targeted with the multifunctional dodecamer. Streptavidin was used to couple biotinylated dodecamer to a biotinylated anti-A. actinomycetemcomitans antibody. This modular targeting approach enabled us to increase the loading of photosensitizer onto the cells by a cycle of amplification. Scanning laser confocal microscopy was used to characterize transport of fluorescently tagged dodecamer into the microcolonies and targeting of the cells with biotin-labeled, ...
Aggregatibacter actinomycetemcomitans GroEL Protein Promotes Conversion of Human CD4 T Cells into IFNγ IL10 Producing Tbet Th1 Cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
ID C9R3L9_AGGAD Unreviewed; 404 AA. AC C9R3L9; DT 24-NOV-2009, integrated into UniProtKB/TrEMBL. DT 24-NOV-2009, sequence version 1. DT 25-OCT-2017, entry version 39. DE SubName: Full=Molybdopterin molybdenumtransferase {ECO:0000313,EMBL:ACX82405.1}; GN OrderedLocusNames=D11S_1018 {ECO:0000313,EMBL:ACX82405.1}; OS Aggregatibacter actinomycetemcomitans serotype C (strain D11S-1) OS (Actinobacillus actinomycetemcomitans). OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; OC Pasteurellaceae; Aggregatibacter. OX NCBI_TaxID=668336 {ECO:0000313,EMBL:ACX82405.1, ECO:0000313,Proteomes:UP000002569}; RN [1] {ECO:0000313,EMBL:ACX82405.1, ECO:0000313,Proteomes:UP000002569} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=D11S-1 {ECO:0000313,EMBL:ACX82405.1, RC ECO:0000313,Proteomes:UP000002569}; RX PubMed=19820097; DOI=10.1128/JB.01203-09; RA Chen C., Kittichotirat W., Si Y., Bumgarner R.; RT "Genome sequence of Aggregatibacter actinomycetemcomitans serotype c RT strain D11S-1."; ...
Methane is metabolized principally by methanotrophs and methanogens in the global carbon cycle. Methanotrophs consume methane as the only source of carbon, while methanogens produce methane as a metabolic byproduct. Methylotrophs, which are microorganisms that can obtain energy for growth by oxidizing one-carbon compounds, such as methanol and methane, are situated between methanotrophs and methanogens. Methanogens can obtain energy for growth by converting a limited number of substrates to methane under anaerobic conditions. Three types of methanogenic pathways are known: CO2 to methane [MD:M00567], methanol to methane [MD:M00356], and acetate to methane [MD:M00357]. Methanogens use 2-mercaptoethanesulfonate (CoM; coenzyme M) as the terminal methyl carrier in methanogenesis and have four enzymes for CoM biosynthesis [MD:M00358]. Coenzyme B-Coenzyme M heterodisulfide reductase (Hdr), requiring for the final reaction steps of methanogenic pathway, is divided into two types: cytoplasmic HdrABC in ...
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The focus of my research is the identification and characterization of bacterial proteins that are required for tissue colonization, the initiation of any infection. My research interests are concentrated on the membrane proteins and surface structures of the Gram-negative bacterium Aggregatibacter actinomycetemcomitans. A. actinomycetemcomitans is typically associated with Periodontitis, an inflammatory disease of the tissues surrounding and supporting the teeth, which left untreated results in tooth loss. This bacterium is also associated with numerous non-oral diseases including but not restricted to infective endocarditis and atherosclerosis, which suggest that the periodontal pocket is a potential source and reservoir of these diseases. The emphasis of my research is based on two genes and the associated proteins identified in my laboratory. We have identified and characterized a unique collagen adhesin expressed by A. actinomycetemcomitans. The extracellular matrix protein adhesin A (EmaA) ...
Monoclonal antibody against Aggregatibacter actinomycetemcomitans serotype b expressed by for use in Western Blot against gram-negative bacteria
What causes rheumatoid arthritis?. The search, by scientists around the world, has been exhaustive, and the prey surprisingly elusive - but in breakthrough Johns Hopkins research, scientists Maximilian Konig, M.D., and Felipe Andrade, M.D., Ph.D., believe they have pinpointed one culprit: a kind of bacteria that lives in the mouth and is present in gum disease.. This form of bacteria has an unpronounceable name, Aggregatibacter actinomycetemcomitans, so the scientists who have discovered the terrible and important role it might play in RA just call it by its initials: Aa. "We are very excited about it," says Konig. If their hypothesis is verified by other scientists and in larger studies, the possibilities of preventing RA from developing, and of treating early RA and possibly reversing symptoms, are enormous. This work was presented at the Plenary Session of the American College of Rheumatologys Annual Scientific Meeting in November 2016, and I recently wrote about it for Leap magazine, a ...
Immunological detection of antigens of Aggregatibacter actinomycetemcomitans, P intermedia, P gingivalisusing antibodies (ELISA ...
Posttranslational modification (PTM) of proteins is a versatile cellular process to regulate the activities of proteins. These modifications are mostly brought about by enzymes that regioselectively introduce a not-genetically encoded functionality at one or several specific proteins. Very interestingly, PTMs are also utilized by bacterial pathogens to manipulate the activities of such host proteins that could potentially interfere with the survival and replication of infectious agents. Since small GTPases are major regulators of intracellular signaling, they are not surprisingly targeted frequently by pathogenic proteins in order to divert cellular defense mechanisms. In this respect, the recent discovery of reversible adenylylation and phosphocholination of the Rab1 subfamily of small GTPases by Legionella pneumophila has been very insightful since these PTMs differently affect Rab biology [1, 2]. Also, adenylylation of the Rho family by enzymes from Histophilus somni and Vibrio ...
Pasteurelose é uma doença infecciosa causada por bactérias do gênero Pasteurella que afeta humanos e animais. Kuhnert P; Christensen H (eds). (2008). Pasteurellaceae: Biology, Genomics and Molecular Aspects. [S.l.]: Caister Academic Press. ISBN 978-1-904455-34-9 ...
Infectious coryza (IC) is a respiratory disease primarily affecting chickens and occasionally pheasants and guinea-fowl. The disease is caused by Avibacterium paragallinarum, a gram-negative nonmotile bacterium.. ...
72 recipes using durum semolina flour. From Baklava with Cream Filling (Muhallebili Baklava) to Fresh Pasta with Tomatoes and Calamari.
Well, I have committed fic. This is actually the second short fic I wrote recently, in this new and wonderful return-to-overt-fannishness, but its the one thats most ready for light of day and I want to shove it outside while I still feel indulgently fond of it. The first fic had no dialogue, which made me freak out about whether I could still write dialogue, so this one has rather a lot of it. This will be, I think, my first fic posted since 2003. I definitely feel rusty at writing, but hopefully its only up from here :D ...
Well, I have committed fic. This is actually the second short fic I wrote recently, in this new and wonderful return-to-overt-fannishness, but its the one thats most ready for light of day and I want to shove it outside while I still feel indulgently fond of it. The first fic had no dialogue, which made me freak out about whether I could still write dialogue, so this one has rather a lot of it. This will be, I think, my first fic posted since 2003. I definitely feel rusty at writing, but hopefully its only up from here :D ...
The purpose of this study was to test the hypothesis that diabetes aggravates periodontal destruction induced by Aggregatibacter actinomycetemcomitans infection. Thirty-eight diabetic and 33 normal rats were inoculated with A. actinomycetemcomitans and euthanized at baseline and at 4, 5, and 6 weeks after inoculation. Bone loss and the infiltration of polymorphonuclear leukocytes (PMNs) in gingival epithelium were measured in hematoxylin-eosin-stained sections. The induction of tumor necrosis factor alpha (TNF-alpha) was evaluated by immunohistochemistry and of apoptotic cells by a TUNEL (terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling) assay. After A. actinomycetemcomitans infection, the bone loss in diabetic rats was 1.7-fold and the PMN infiltration 1.6-fold higher than in normoglycemic rats (P , 0.05). The induction of TNF-alpha was 1.5-fold higher and of apoptotic cells was up to 3-fold higher in diabetic versus normoglycemic rats (P , 0.05). Treatment with a ...
ABSTRACT. It is suggested that Haemophilus paragallinarum requires at least three haemagglutinins for adhesion during infection. This paper reports the partial purification and characterization of the HA-L haemagglutinin from H. paragallinarum strain 46-C3, a heat sensitive, trypsin sensitive haemagglutinin that has been shown to be the serovar specific haemagglutinin in this organism. Using the pi and molecular mass obtained, it was shown that this protein shares similarities with other types of adhesins found in Gram-negative bacteria. The haemagglutination assay conditions were optimized at pH 7.5 at 37 °C. It was also shown that activity is enhanced by the addition of Ca2+ and Mn2+ ions.. Keywords: Haemagglutinin purification and characterization, Haemophilus paragallinarum. ...
Introduction. Respiratory disease has been a major mortality factor in Rocky Mountain bighorn sheep (Ovis canadensis canadensis) populations in North America and has been implicated as the major cause of population declines since the 1800s (Rush, 1927, Beuchner, 1960, Post 1962, Stelfox, 1971, Schwantje, 1988). Disease epizootics can progress rapidly and affect all ages of animals resulting in massive die-offs and reduced lamb recruitment for years afterward (Potts, 1937, Marsh, 1938, Spraker et al., 1984, Thorne et al., 1982).. Bacteria in the family Pasteurellaceae, primarily Mannheimia (Pasteurella) haemolytica, Bibersteinia trehalosi, and P. multocida have been implicated as critical pathogens associated with pneumonic epizootics in bighorn sheep (Schwantje, 1988, Rudolph et al. 2003, Weiser et al., 2003). A portion of the organisms previously identified as Pasteurella haemolytica have been assigned to the new genus Mannheimia using primarily 16S rRNA sequencing along with other molecular ...
Historically, the causative agent of infectious coryza has been identified as the NAD requiring bacterium Haemophilus paragallinarum and the implementation of an intensive vaccination program led to the effective control of this contagious upper respiratory infection. More recently, however, a decline in the protective capacity of a vaccine conditioned immune response was noted, with a number of contributing factors, including the emergence of a fast-growing NAD-independent bacterium, which has largely replaced the traditional NAD-dependent variety. As such, accurate, reproducible methods for determining and continually monitoring the type of infecting bacteria was necessitated. To address this need, strains of H. paragallinarum were evaluated according to both their phenotypic and their genotypic properties, in a combination serodiagnostic approach. A data bank of NAD-dependent H. paragallinarum reference strain and field isolate serovar-specific fingerprints was established on both a whole ...
Sockett and Lambert, 2004. Balos are ubiquitous in nature and have been isolated from diverse terrestrial and aquatic habitats, from biofilms as well as from animal feces
PubMed journal article Serotyping of Haemophilus paragallinarum isolates from Mexico by the Kume hemagglutinin schem were found in PRIME PubMed. Download Prime PubMed App to iPhone or iPad.
The HMWABC system of non-typeable Haemophilus influenzae (NTHi) encodes the HMWA adhesin glycoprotein, which is glycosylated by the HMWC glycosyltransferase. HMWC is a cytoplasmic N-glycosyltransferase, homologues of which are widespread in the Pasteurellaceae. We developed an assay for nonbiased detection of glycoproteins in NTHi based on metabolic engineering of the Leloir pathway and growth in media containing radiolabelled monosaccharides. The only glycoprotein identified in NTHi by this assay was HMWA. However, glycoproteomic analyses ex vivo in Escherichia coli showed that HMWC of NTHi was a general glycosyltransferase capable of glycosylating selected asparagines in proteins other than its HMWA substrate, including Asn78 in E. coli 30S ribosomal protein S5. The equivalent residue in S5 homologues in H. influenzae or other sequenced Pasteurellaceae genomes is not asparagine, and these organisms also showed significantly fewer than expected potential sites of glycosylation in general. ...
Johns Hopkins Medicine (JHM). December 14, 2016. Investigators at Johns Hopkins report they have new evidence that a bacterium known to cause chronic inflammatory gum infections also triggers the inflammatory "autoimmune" response characteristic of chronic, joint-destroying rheumatoid arthritis (RA). The new findings have important implications for prevention and treatment of RA, say the researchers.. In a report on the work, published in the Dec. 14 edition of the journal Science Translational Medicine, the investigators say the common denominator they identified in periodontal disease (gum disease) and in many people with RA is Aggregatibacter actinomycetemcomitans. An infection with A. actinomycetemcomitans appears to induce the production of citrullinated proteins, which are suspected of activating the immune system and driving the cascade of events leading to RA.. "This is like putting together the last few pieces of a complicated jigsaw puzzle that has been worked on for many years," says ...
Aggregatibacter actinomycetemcomitans (Aa) expresses a 64-kDa GroEL protein belonging to the heat shock family of proteins. This protein has been shown to influence human host cells, but the apoptotic capacity of the GroEL protein regarding T cells is not yet known. The purpose of this study was to investigate the ability of A. actinomycetemcomitans GroEL (AaGroEL) protein to induce human peripheral blood T-cell apoptosis. Endogenous, purified AaGroEL protein was used as an antigen. In AaGroEL-treated T cells, the data indicated that phosphatidylserine exposure, an early apoptotic event, was dose- and time-dependent. The AaGroEL-treated T cells were also positive for active caspase-3 in a dose-dependent manner. The rate of AaGroEL-induced apoptosis was suppressed by the addition of the general caspase inhibitor Z-VAD-FMK. Furthermore, cleaved caspase-8 bands (40/36 kDa and 23 kDa) were identified in cells responding to AaGroEL. DNA fragmentation was also detected in the AaGroEL-treated T cells. ...
I have so many great memories of growing up with Farina or Cream Of Wheat, also known as Wheat Farina or Semolina. It was a huge stable of my diet as a kid and I cannot remember ever not liking my bowl of Farina topped with cinnamon and sugar. Organic Semolina is actually pretty prefect for growing bodies and it provides your body with extra energy.. During my first pregnancy I switched to almost everything organic in my diet - and it was during that time when I discovered organic hot cereal made from organic wheat. I wanted to avoid the extra additives and pesticides from conventional brands. I was super surprised to discover that the Organic Wheat Cereal tasted even better and creamier than their conventional counterparts. Over the last few years I have stepped away from many dairy products and I prefer a non-dairy milk anytime of day over regular cows milk . My digestion feels so much better and it makes me feel less weighed down. Needless to say that I have learned to prepare my hot cereal ...
Accepted name: dTDP-4-dehydro-6-deoxyglucose reductase. Reaction: dTDP-α-D-fucopyranose + NAD(P)+ = dTDP-4-dehydro-6-deoxy-α-D-glucose + NAD(P)H + H+. For diagram of reaction click here.. Glossary: dTDP-4-dehydro-6-deoxy-α-D-glucose = dTDP-6-deoxy-α-D-xylo-hexopyranos-4-ulose = thymidine 5-[3-(6-deoxy-D-xylo-hexopyranosyl-4-ulose) diphosphate]. Other name(s): dTDP-4-keto-6-deoxyglucose reductase; dTDP-D-fucose:NADP+ oxidoreductase; Fcf1; dTDP-6-deoxy-D-xylo-hex-4-ulopyranose reductase. Systematic name: dTDP-α-D-fucopyranose:NAD(P)+ oxidoreductase. Comments: The enzymes from the Gram-negative bacteria Aggregatibacter actinomycetemcomitans and Escherichia coli O52 are involved in activation of fucose for incorporation into capsular polysaccharide O-antigens [1,3]. The enzyme from the Gram-positive bacterium Geobacillus tepidamans is involved in activation of fucose for incorporation into the organisms S-layer [2].The enzyme from Escherichia coli O52 has a higher catalytic efficiency with ...
I realised Id made a huge error when mixing this all together, seeing that it was all the yellow corn at the bottom and all the milk floating on top unintegrated, so I looked at the packaging again and seeing that it says sémola de maíz (Im living in Spain) I looked up if there was a difference between corn semolina and cornmeal... Turns out, big difference! In a rescue attempt I mixed in 3 tablespoons cornstarch (I think you call this cornflour in the States) and heated it on the hob for a few minutes until it was pretty much cooked polenta. After 20 minutes in the oven, this turned out pretty tasty, and I will definitely make it again. Im not sure if I would have achieved the same result by simply cooking corn semolina and milk then baking it though, or did the extra ingredients still add something to it do you think ...
How to make Onion rings dipped in flour batter, coated with spiced semolina and deep fried.-Onion rings dipped in flour batter, coated with spiced semolina and deep fried.
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Alb-Gold Organic Kids Pasta Safari - This Alb-GOld Oragnic Kids Pasta is made from the highest quality organic durum wheat semolina. Thi
Things to do near Battleship USS ALABAMA on TripAdvisor: See 14,963 reviews and 4,403 candid photos of things to do near Battleship USS ALABAMA in Mobile, Alabama.
Ilmateade lubas küll kesknädalaks paksu vihmasadu, kuid eksis õnneks oluliselt ning Pirita lähistel merel kohtunud purjetajaid soosis taaskord kaunis suveilm - päike paistis rõõmsalt ning värske läänetuul tervitas merelisi reipalt. Vastupidiselt eelmisel hooajal purjetajaid kimbutanud ilma-vingerpussidele, on käesolev kevadsuvi väga hoidnud USS Security-KJK kolmapäevaregattide osalisi. Kesknädalast seljasirutust ja oodatud pingutust jahtide pardail olid tulnud tegema 33 purjeka meeskonnad neljas arvestusgrupis. - DELFI
Now at a discount, get this deal on the Rainbird DG7USS 7 Universal Square Drainage Grate (Sand) via PlumbersStock. We have lots of Rain Bird.
Ha ha! What I create I can also destroy! As promised, I tore all of the hull plates off and will give it a second try.:twisted: [IMG] I...
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Pasteurella canis is a Gram-negative, nonmotile, penicillin-sensitive coccobacillus belonging to the Pasteurellaceae family. Bacteria from this family cause zoonotic infections in humans, which manifest themselves as skin or soft-tissue infections after an animal bite. It has been known to cause serious disease in immunocompromised patients. Pasteurella was first described around 1880 and thought to be associated with chicken cholera and hemorrhagic septicemia in animals. The genus was first cultured in 1885. In 1920, strains of Pasteurella were isolated and observed in human infections. "Micrococcus gallicidus" was the first scientific name used for these bacteria. For a short time, the bacteria were unofficially placed in different genera, such as Octopsis, Coccobacillus, and Eucystia. In 1887, genus "Pasteurella" was proposed to honor Louis Pasteur for his critical discoveries in the field of microbiology. Before molecular technology was available, species were often labeled based on staining ...
Virulent strains of the bovine opportunistic pathogen Haemophilus somnus (Histophilus somni) cause multi-systemic diseases in cattle. One of the reported virulence factors that H. somnus may use to persist in the host is resistance to intracellular killing. It is reported in this dissertation that H. somnus significantly (P ...
Curated}} {{Biorealm Genus}} [[Image:pasteurella.jpg,thumb,400px,right,Pasteurella multocida. From [http://www.geocities.com/CapeCanaveral/3504/gallery.htm Neal Chamberlain.]]] ==Classification== ===Higher order taxa:=== Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae ===Species:=== Pasteurella aerogenes; P. avium; P. bettyae; P. caballi; P. canis; P. dagmatis; P. gallinarum; P. langaaensis; P. mairii; P. multocida; P. pneumotropica; P. skyensis; P. stomatis; P. testudinis; P. trehalosi; P. volantium; P. sp. {, , height="10" bgcolor="#FFDF95" align="center" , NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=745&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy] [http://www.ncbi.nlm.nih.gov/genomes/framik.cgi?db=Genome&gi=170 Genome] ,} ==Description and Significance== Pasteurella pneumotropica is an opportunistic pathogen that can be often found in many commerical and research colonies of rodents. ==Genome Structure== The ...
Serum haptoglobin (Hp) and haptoglobin matrix metalloproteinase 9 complexes (Hp-MMP 9) have been identified as biomarkers with diagnostic potential in cattle with conditions resulting in an acute inflammatory response. The purpose of this study was to evaluate potential diagnostic applications of serum Hp and Hp-MMP 9 concentrations in calves with BRD and establish a timeline for their detection in calves experimentally challenged with Bibersteinia trehalosi and Mannheimia haemolytica. Thirty-five cross bred dairy calves were inoculated via tracheal catheterization with either a PCR confirmed leukotoxin negative B. trehalosi isolate, a PCR confirmed leukotoxin positive B. trehalosi isolate, a Mannheimia haemolyticaisolate, a combination of leukotoxin negative B. trehalosi and M. haemolytica, or a negative control. Serum samples were collected throughout the study. Calves were euthanized and necropsy performed on day 10 post inoculation. M. haemolytica inoculated calves had increased lung involvement.
Horizontal gene transfer is an important source of genetic variation among Neisseria species and has contributed to the spread of resistance to penicillin and sulfonamide drugs in the pathogen Neisseria meningitidis. Sulfonamide resistance in Neisseria meningitidis is mediated by altered chromosomal folP genes. At least some folP alleles conferring resistance have been horizontally acquired from other species, presumably from commensal Neisseriae. In this work, the DNA sequence surrounding folP in commensal Neisseria species was determined and compared to corresponding regions in pathogenic Neisseriae, in order to elucidate the potential for inter-species DNA transfer within this region ...
The Neisseria species are believed to be non-clonal bacteria with a high degree of genetic transfer within and between different species [2, 3, 14-18]. Previous reports of transformation in N. meningitidis have suggested that different regions of the genome may have different recombination rates [19, 20]. This work identified the region upstream of folP as particularly active in recombination events. The most obvious example is the insertion of a complete Correia element, upstream of folP in N. lactamica. Correia elements are small putative transposable elements, around 152 base pairs long with inverted repeats at the ends [12]. Hundreds of Correia copies are scattered around the genomes of pathogenic Neisseriae [13, 21, 22] and they have also been reported in N. lactamica [23]. We detected another copy of a Correia repeat in the N. subflava sequence, suggesting that these elements are ubiquitous in the Neisseria genus and possibly was introduced before the divergence into different ...
The cytolethal distending toxins (CDTs) are a family of exotoxins produced by a wide range of Gram-negative bacteria. They are known for causing genotoxic stress to the cell, resulting in growth arrest and eventually apoptotic cell death. Nevertheless, there is evidence that CDTs can also perturb the innate immune responses, by regulating inflammatory cytokine production and molecular mediators of bone remodeling in various cell types. These cellular and molecular events may in turn have an effect in enhancing local inflammation in diseases where CDT-producing bacteria are involved, such as Aggregatibacter actinomycetemcomitans, Haemophilus ducreyi, Campylobacter jejuni and Helicobacter hepaticus. One special example is the induction of pathological bone destruction in periodontitis. The opportunistic oral pathogen Aggregatibatcer actinoycemetemcomitans, which is involved in the aggressive form of the disease, can regulate the molecular mechanisms of bone remodeling in a manner that favors bone
Obradović, Davor, et al. "A Cytolethal Distending Toxin Variant from Aggregatibacter actinomycetemcomitans with an Aberrant CdtB That Lacks the Conserved Catalytic Histidine 160." PloS one 11.7 (2016): e0159231.. Hemp, James, et al. "Draft Genome Sequence of Ornatilinea apprima P3M-1, an Anaerobic Member of the Chloroflexi Class Anaerolineae." Genome Announcements 3.6 (2015): e01353-15.. Ward, Lewis M., et al. "Draft Genome Sequence of Leptolinea tardivitalis YMTK-2, a Mesophilic Anaerobe from the Chloroflexi Class Anaerolineae." Genome Announcements 3.6 (2015): e01356-15.. Hemp, James, et al. "Draft Genome Sequence of Levilinea saccharolytica KIBI-1, a Member of the Chloroflexi Class Anaerolineae." Genome Announcements 3.6 (2015): e01357-15.. Hemp, James, et al. "Draft Genome Sequence of Ardenticatena maritima 110S, a Thermophilic Nitrate-and Iron-Reducing Member of the Chloroflexi Class Ardenticatenia." Genome Announcements 3.6 (2015): e01347-15.. Pace, Laura A., et al. "Draft Genome of ...
Protease Activated Receptors (PARs) are G-protein coupled receptors and they play an active role in host defense. PAR1 and PAR2 are the most highly expressed members of the PAR family in gingival epithelial cells (GECs). This thesis is focused on the role of these two receptors. Objective: This study investigated how PAR receptors regulate downstream signaling pathways and subsequent cytokines secretion in response to specific bacteria in gingival epithelial innate immunity. Methods: Human GECs were transfected with small interfering RNA (siRNA) specific for PAR1 or PAR2. The cells were then stimulated with perio-pathogenic Porphyromonas gingivalis (PG), Aggregatibacter actinomycetemcomitans (AA) or non-pathogenic bacteria Streptococcus gordonii (SG). We used quantitative real-time polymerase chain reaction (QRT-PCR) to detect the messenger RNA (mRNA) expression level of the different innate immune markers; in addition, we used Multi-ELISA ARRAY to measure the protein level of select cytokines ...
Objectives: To investigate the short-term effects of nonsurgical therapy (scaling and root planing, SRP) on the subgingival microbiota in chronic (CP) and aggressive (AP) perio dontal disease. Method and Materials: Ninety-seven CP and AP subjects underwent full-mouth SRP on 2 consecutive days. AP patients were randomly assigned to either receive systemic metronidazole plus amoxicillin (AP+AB) or were treated mechanically alone (AP). Pathogens were identified with 16S rRNA oligodeoxynucleotide probes and dot-blot hybridization before and at days 2, 3, 4, 7, 10, and 21 of healing. CP subjects were treated by scaling and root planing along with placebo tablets. Results: Initially, AP cell counts were 69.9- (Porphyromonas gingivalis), 10.2- (Aggregatibacter actinomycetemcomitans), 5.7- (Tannerella forsythia), and 3.3-fold (Prevotella intermedia) enhanced compared to CP cell counts. Following SRP, immediate elimination occurred in single individuals of all three treatment groups at day 2. After SRP ...
Materials and Methods: The present study was designed as a prospective stratified randomized controlled clinical trial. Patients were scheduled to receive two implants with different types of abutments in the posterior mandible. Three months after implant placement, titanium and zirconium abutments were connected. Five weeks after abutment connections, the abutments were removed, probing depth measurements were recorded, and gingival biopsy samples were obtained. Abutments and biopsy specimens were analyzed by reverse-transcriptase polymerase chain reaction to compare the DNA copy numbers of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and total bacteria. The surface free energy of the abutments was calculated by sesile water drop before replacement ...
General Information: This is a serogroup A strain isolated in Gambia in 1983. Causes septicemia and meningitis. The second of two pathogenic Neisseria, this organism causes septicemia and is the leading cause of life-threatening meningitis (inflammation of the meninges, the membrane surrounding the brain and spinal cord) in children. This organism typically residies in the nasopharynx cavity but can invade the respiratory epthelial barrier, cross into the bloodstream and the blood brain barrier, and cause inflammation of the meninges. Pathogenicity factors include the surface proteins (porins and opacity proteins), and the type IV pilus (which is also found in Neisseria gonorrhoeae). Pathogenicity factors include the surface proteins (porins and opacity proteins), and the type IV pilus (which is also found in Neisseria gonorrhoeae). This organism, like Neisseria gonorrhoeae, is naturally competent, and protein complexes at the cell surface recognize the uptake signal sequence in extracellular ...
General Information: This is a serogroup A strain isolated in Gambia in 1983. Causes septicemia and meningitis. The second of two pathogenic Neisseria, this organism causes septicemia and is the leading cause of life-threatening meningitis (inflammation of the meninges, the membrane surrounding the brain and spinal cord) in children. This organism typically residies in the nasopharynx cavity but can invade the respiratory epthelial barrier, cross into the bloodstream and the blood brain barrier, and cause inflammation of the meninges. Pathogenicity factors include the surface proteins (porins and opacity proteins), and the type IV pilus (which is also found in Neisseria gonorrhoeae). Pathogenicity factors include the surface proteins (porins and opacity proteins), and the type IV pilus (which is also found in Neisseria gonorrhoeae). This organism, like Neisseria gonorrhoeae, is naturally competent, and protein complexes at the cell surface recognize the uptake signal sequence in extracellular ...
0084] In a preferred embodiment the presence of Gallibacterium is determined by an immune test. An immune test uses monoclonal antibodies or polyclonal antisera specific to Gallibacterium. The generation of monoclonal antibodies is known in the art (Kearney, J. F., Radbruch A., Liesegang B., Rajewski K. A new mouse myeloma cell line that has lost immunoglobulin expression but permits construction of antibody-secreting hybrid cell lines. J. Immunol. 1979, 123: 1548-1550., Kohler, G., Milstein, C. Continous culture of fused cells secreting antibody of predefined specifity. Nature 1975, 265: 495-497) Immune tests include the methods of detection known in the art such as the ELISA test (enzyme-linked immuno-sorbent assay) or the so-called sandwich-ELISA test, dot blots, immunoblots, radioimmuno tests (radioimmunoassay RIA), diffusion-based Ouchterlony test or rocket immunofluorescent assays) or agglutination tests (rapid plate or micro-plate agglutination tests). Another immune test is the so-called ...
Nitronal Aqueous information about active ingredients, pharmaceutical forms and doses by Pohl Boskamp, Nitronal Aqueous indications, usages and related health products lists
aae c9335 manufacturers and aae c9335 suppliers Directory - Find aae c9335 Manufacturers, Exporters and aae c9335 suppliers on ECVERY.com
North African flavors marinate tender lamb that you grill and serve with hot cooked couscous, the wheat-grain semolina served as a grain side dish in this part of the world.
Three generations of pasta makers have crafted the perfect ravioli. Enriched semolina pasta filled with premium creamy ricotta, mozzarella, freshly grated Asiago, fontina, Parmesan and Romono cheeses. Top with Schwans vegetables, meat and sauces for a delicious home made tasting meal.
Diverses aplicacions del KDE us permet desar i carregar sessions. KRunner us permetrà llançar aquestes sessions. Podeu escriure en el nom de laplicació o escriure directament en el nom de la sessió. Hi ha executors per obrir les sessions desades en el Kate, Konqueror, Konsole, etc. KRunner és un somni de la multitasca. Si teniu masses finestres obertes, simplement introduïu el títol de la finestra i passareu directament a ella. Igualment, podeu escriure el nom dun escriptori virtual i passar directament a ell. Si treballeu amb les activitats, alternareu entre elles escrivint el seu nom. KRunner trobarà coincidència tan aviat com escriviu tres caràcters. KRunner fins i tot alternarà entre sessions descriptori. Escriviu la paraula clau ...
Buckley, L. E. R. - Coe, B. J. - Rusanova, D. - Sánchez, S. - Jirásek, Michael - Joshi, Vishwas - Vávra, Jan - Khobragade, Dushant - Pospíšil, Lubomír - Ramešová, Šárka - Císařová, I. - Šaman, David - Pohl, Radek - Clays, K. - Van Steerteghem, N. - Brunschwig, B. S. - Teplý, Filip ...
Your email address will not be shared with anyone who doesnt have a subpoena, a slice of cheesecake, AND a working hoverboard.. ...
Mannheimia haemolytica is an opportunistic pathogen in cattle and the main bacterial agent in bovine respiratory disease. Despite its economic importance, few studies have characterized the genetic diversity of M. haemolytica, particularly from feedlots. Three genotyping techniques (BOX-PCR, (GTG)5-PCR and PFGE) were compared to discriminate M. haemolytica and strains from the family Pasteurellaceae. PFGE was the most discriminating and repeatable, although BOX-PCR was most accurate in clustering isolates together according to species. Mannheimia haemolytica was isolated from nasal swab samples collected from cattle upon entry and exit from two feedlots in southern Alberta. These were characterized by PFGE and antimicrobial susceptibility using a disk-diffusion assay. Select gene determinants were screened for using PCR. PFGE analysis revealed the isolates to be highly diverse. Ten percent of the isolates exhibited resistance. At present, the development and spread of antimicrobial resistance in ...
The complete nucleotide sequence of a 7.7kb mobilisable plasmid (pM3446F), isolated from a florfenicol resistant isolate of Actinobacillus pleuropneumoniae, showed extended similarity to plasmids found in other members of the Pasteurellaceae containing the floR gene as well as replication and mobilisation genes. Mobilisation into other Pasteurellaceae species confirmed that this plasmid can be transferred horizontally ...
A usually acute, sometimes chronic, highly infectious disease of chickens, occasionally pheasants and guinea-fowl, characterised by catarrhal inflammation of the upper respiratory tract, especially nasal and sinus mucosae. Infectious Coryza is caused by the bacterium Haemophilus paragallinarum and is seen in many countries especially in multi-age farms that are never depopulated. Morbidity is high but mortality low if uncomplicated although it may be up to 20%. This disease causes aasmus, swollen head, several bacteria in respiratory tract.
The assumption that USS arise by mutation rather than copying was first confirmed using alignments of H. influenzae proteins with distant homologues. Abundant USS-like repeats were found in all eight Pasteurellacean genomes; the repeat consensuses of species in the Hin subclade were identical to that of H. influenzae (AAGTGCGGT), whereas members of the Apl subclade shared the consensus ACAAGCGGT. All species USSs had the strong consensus and flanking AT-rich repeats of H. influenzae USSs. DNA uptake and competition experiments demonstrated that the Apl-type repeat is a true USS distinct from the Hin-type USS: A. pleuropneumoniae preferentially takes up DNA fragments containing the Apl-type USS over both H. influenzae and unrelated DNAs, and H. influenzae prefers its own USS over the Apl type ...
2016-present: Pilot project on strategic partnership with Denmark on data quality (EFSA). 2014: Project management, writing and editing chapters the EU Summary report on Zoonoses 2012, and Antimicrobial resistance in Salmonella and Enterococcus (EFSA).. 2013 - 2015: Critical review of methodology and application of risk ranking for prioritisationof food and feed related issues, on the basis of the size of anticipated health impact. Review of risk ranking papers regarding disease burden (DALY/QALY) and nutrition (EFSA).. 2014 - present: Reporting officer of antimicrobial resistance data to EFSA Data Collection Framework 2013: Project management, writing and editing Salmonella and Listeria chapters the EU Summary report on Zoonoses 2012 (EFSA).. 2012 - present: Danish representative in Emerging Risks Exchange Network (EFSA).. 2011; Data management and writing report on analyses of data on microbiological contaminants in food in EU in years 2004-2009 (EFSA). 2008-2009: Working group on monitoring ...
Looking for online definition of AAE in the Medical Dictionary? AAE explanation free. What is AAE? Meaning of AAE medical term. What does AAE mean?
Felix, JF., Bradfield, JP., Monnereau, C., van der Valk, RJ., Stergiakouli, E., Chesi, A., Gaillard, R., Feenstra, B., Thiering, E., Kreiner-Mller, E., Mahajan, A., Pitknen, N., Joro, R., Cavadino, A., Huikari, V., Franks, S., Groen-Blokhuis, MM., Cousminer, DL., Marsh, JA., Lehtimki, T., Curtin, JA., Vioque, J., Ahluwalia, TS., Myhre, R., Price, TS., Vilor-Tejedor, N., Yengo, L., Grarup, N., Ntalla, I., Ang, W., Atalay, M., Bisgaard, H., Blakemore, AI., Bonnefond, A., Carstensen, L. Bone Mineral Density in Childhood Study (BMDCS) Consortium; Early Genetics and Lifecourse Epidemiology (EAGLE) consortium, Eriksson, J., Flexeder, C., Franke, L., Geller, F., Geserick, M., Hartikainen, AL., Haworth, CM., Hirschhorn, JN., Hofman, A., Holm,, Human Molecular Genetics, ...
Demenais F, Margaritte-Jeannin P, Barnes KC, Cookson WOC, Altmüller J, Ang W, Barr RG, Beaty TH, Becker AB, Beilby J, Bisgaard H, Bjornsdottir US, Bleecker E, Bønnelykke K, Boomsma DI, Bouzigon E, Brightling CE, Brossard M, Brusselle GG, Burchard E, Burkart KM, Bush A, Chan-Yeung M, Chung KF, Couto Alves A, Curtin JA, Custovic A, Daley D, de Jongste JC, Del-Rio-Navarro BE, Donohue KM, Duijts L, Eng C, Eriksson JG, Farrall M, Fedorova Y, Feenstra B, Ferreira MA, Freidin MB, Gajdos Z, Gauderman J, Gehring U, Geller F, Genuneit J, Gharib SA, Gilliland F, Granell R, Graves PE, Gudbjartsson DF, Haahtela T, Heckbert SR, Heederik D, Heinrich J, Heliövaara M, Henderson J, Himes BE, Hirose H, Hirschhorn JN, Hofman A, Holt P, Hottenga J, Hudson TJ, Hui J, Imboden M, Ivanov V, Jaddoe VWV, James A, Janson C, Jarvelin MR, Jarvis D, Jones G, Jonsdottir I, Jousilahti P, Kabesch M, Kähönen M, Kantor DB, Karunas AS, Khusnutdinova E, Koppelman GH, Kozyrskyj AL, Kreiner E, Kubo M, Kumar R, Kumar A, Kuokkanen ...
Niehuis, O.*, G. Hartig*, S. Grath*, H. Pohl, J. Lehmann, H. Tafer, A. Donath, V. Krauss, C. Eisenhardt, J. Hertel, M. Petersen, C. Mayer, K. Meusemann, R. S. Peters, P. F. Stadler, R. G. Beutel, E. Bornberg-Bauer, D. D. McKenna, and B. Misof (2012 ...
Hello, all! :D Hope you all are or have had a great holiday! Well, Barry, the bloopers were: 1) the barrel ring on the carronade apparently fell...
What are your best practices to prevent bacteria and biofilm growth in your NICU feeding tubes? These studies demonstrate what seems to make a difference.
This is a pretty damn awesome premise for a fic :) That being said, this chapter is very dialogue-heavy, with punctuation errors throughout that make it difficult to follow in places. Perhaps a go-through with a beta-reader would help, because you really have the potential for a great fic here. Good luck ...
Aggregatibacter (Actinobacillus) actinomycetemcomitans is a bacterium mainly associated with aggressive forms of periodontitis. Among its virulence factors, a leukotoxin is suggested to play an important role in the pathogenicity. Periodontal infections with strains producing high levels of the leukotoxin are strongly associated with severe disease. Leukotoxin selectively kills human leukocytes and can disrupt the local defense mechanisms. Previous studies examining the role of the leukotoxin in host-parasite interactions have mainly focused on polymorphonuclear leukocytes (PMNs). In the inflamed periodontium, macrophages play a significant role in the regulation of the inflammatory reactions and the tissue breakdown and remodeling.. Thus, the aim of this dissertation was to investigate death mechanisms of human macrophages exposed to leukotoxin.. Human lymphocytes, PMNs, and monocytes/macrophages isolated from venous blood were exposed to purified leukotoxin or live A. actinomycetemcomitans ...
THE EFFECT OF CURCUMA XANTHORRHIZA ETHANOL EXTRACT ON THE VIABILITY OF STREPTOCOCCUS MUTANS AND AGGREGATIBACTER ACTINOMYCETEMCOMITANS (DENTAL BIOFILM RESEARCH: IN VITRO STUDY)
R.L. Larson, D.L. Step, Evidence-Based Effectiveness of Vaccination Against Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni in Feedlot Cattle for Mitigating the Incidence and Effect of Bovine Respiratory Disease Complex, Veterinary Clinics of North America: Food Animal Practice, 2012, 28, 1, ...
The main objective of this work was to assess the antimicrobial properties and the dentin-bonding strength of novel magnesium phosphate cements (MPC). Three formulations of MPC, consisting of magnesium oxide and a phosphate salt, NH4H2PO4, NaH2PO4 or a mixture of both, were evaluated. As a result of the setting reaction, MPC transformed into either struvite (MgNH4PO4·6H2O) when NH4H2PO4 was used or an amorphous magnesium sodium phosphate when NaH2PO4 was used. The MPC had appropriate setting times for hard tissue applications, high early compressive strengths and higher strength of bonding to dentin than commercial mineral trioxide aggregate cement. Bacteriological studies were performed with fresh and aged cements against three bacterial strains, Escherichia coli, Pseudomonas aeruginosa (planktonic and in biofilm) and Aggregatibacter actinomycetemcomitans. These bacteria have been associated with infected implants, as well as other frequent hard tissue related infections. Extracts of different ...
Actinobacillus pleuropneumoniae, a Gram-negative, non-motile, facultatively anaerobic Pasteurellaceae, causes porcine pleuropneumonia, a highly contagious disease for which there is no effective vaccine. There are least twelve different serotypes, some of which produce no disease but others cause severe disease. Serotypes vary in different countries. Types 1, 5, 9, 11 and 12 are usually highly virulent and strains 3 and 6 are mild. The bacterium is carried in the tonsils and upper respiratory tract. It is transmitted short distances by droplet infection and only survives outside the pig for a few days.Actinobacillus pleuropneumoniae strain JL03 is an isolate of serotype 3 which is prevalent in China. Its genome is made up of a single circular chromosome encoding 2097 CDSs. It possesses a complete set of genes coding for enzymes of glycolysis, gluconeogenesis and non-oxidative pentose phosphate pathways. Genes coding for the key enzymes of the TCA cycle, citrate synthase, aconitase and isocitrate ...
My mothers Revani Recipe Cake 3 large eggs 1 cup sugar Whisk eggs and sugar by electric mixer 1 cup coarse semolina 1 cup flour 1 teaspoon (totally full) baking powder 1 cup yogurt Stir semolina, flour and baking powder by hand Once they are mixed poor into whisked eggs and sugar, and mixed them by hand. Last, add yogurt then mix all of them by hand Pre-heat oven 275 F Poor the cake batter into buttered 9"x13" regular pan. Bake the cake for about 25-30 min. Syrup 3 cup sugar 3 cup water Boil sugar and water in a saucepan. When it is boiled add 2 table spoon lemon-juice and let the syrup boil another 2 minutes. Then, Cool it completely. Slowly poor cooled syrup all over the hot cake (as soon as you finished baking). As youd like, you can topped with shredded coconut or whipped cream before you serve. Enjoy your Revani! ...
Food Science & Nutrition. ORIGINAL RESEARCH. Chemical and functional properties of cassava starch, durum wheat semolina flour, and their blends. Olufunmilola O. Oladunmoye1, Ogugua C. Aworh2, Bussie Maziya-Dixon3, Ochuko L. Erukainure1 & Gloria N. Elemo1. 1Food Technology Department, Federal Institute of Industrial Research, Oshodi, Lagos, Nigeria. department of Food Technology, University of Ibadan, Ibadan, Nigeria. 3Crop Utilization Unit, International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria. Keywords. Cassava starch, durum wheat semolina, flour blends, functional properties. Correspondence. Olufunmilola O. Oladunmoye, Food Technology Department, Federal Institute of Industrial Research, Oshodi, Lagos, Nigeria. Tel: +234 802 354 0666; E-mail: [email protected] Funding Information. Analyses made in this study were partially supported by the Crop Utilization Unit of the International Institute of Tropical Agriculture (IITA), Ibadan and Quality Control Laboratory of Flour ...
For use in healthy cattle and calves three months of age or older as an aid in preventing blackleg caused by Clostridium chauvoei, malignant edema caused by Cl. septicum, black disease caused by Cl. novyi, gas-gangrene caused by Cl. sordellii, enterotoxemia and enteritis caused by Cl. perfringens Types B, C and D, and disease caused by Histophilus somni (Haemophilus somnus). Although Cl. perfringens Type B is not a significant problem in North America, immunity is provided by the beta toxoid of Type C and the epsilon toxoid of Type D. Ultrabac 7/Somubac consists of killed, standardized cultures of Cl. chauvoei, Cl. septicum, Cl. novyi, Cl. sordellii, Cl. perfringens Types C and D, and H. somni, with an adjuvant. Available in a 10 dose or 50 dose.. ...
Background: The aim of this investigation was to quantify periodontal pathogens (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Campylobacter rectus and Tannerella forsythia) in vascular, blood and subgingival samples. As secondary objective, two molecular bacterial identification methods [nested-polimerase chain reaction (PCR) and quantitative PCR (qPCR)] were compared.. Methods: Seventy consecutive patients provided a vascular lesion, a blood sample, and 36 subgingival samples. Bacterial deoxyribonucleic acid (DNA) was extracted and qPCR was used to determine the prevalence and amounts of the target pathogens in each sample. Nested-PCR was only performed in the samples from vascular lesions. Periodontal examination was performed in 42 patients. U-Mann-Whitney or Chi-squared tests were used to compare microbiological results according to periodontal diagnosis.. Results: All targeted periodontal pathogens (A. actinomycetemcomitans, P. gingivalis, T. forsythia or C. rectus) were ...
Looking for online definition of Actinobacillus actinomycetemcomitans in the Medical Dictionary? Actinobacillus actinomycetemcomitans explanation free. What is Actinobacillus actinomycetemcomitans? Meaning of Actinobacillus actinomycetemcomitans medical term. What does Actinobacillus actinomycetemcomitans mean?

Pasteurellaceae - WikipediaPasteurellaceae - Wikipedia

The Pasteurellaceae comprise a large family of Gram-negative bacteria. Most members live as commensals on mucosal surfaces of ... Pasteurellaceae are typically rod-shaped, and are a notable group of facultative anaerobes. Their biochemical characteristics ... Comparative analyses of Pasteurellaceae genomes have identified large numbers (>20) of conserved signature indels (CSIs) in ... Based upon many other CSIs that are specific for subgroups of Pasteurellaceae species, it has been proposed to divide the ...
more infohttps://en.wikipedia.org/wiki/Pasteurellaceae

Pasteurella - microbewikiPasteurella - microbewiki

Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae Species:. Pasteurella aerogenes; P. avium; P. ...
more infohttps://microbewiki.kenyon.edu/index.php?title=Pasteurella&printable=yes

USCC 2216 Strain Passport - StrainInfoUSCC 2216 Strain Passport - StrainInfo

Phylogeny of the Pasteurellaceae as determined by comparison of 16S ribosomal ribonucleic acid sequences ... Comparative phylogenies of the housekeeping genes atpD, infB and rpoB and the 16S rRNA gene within the Pasteurellaceae ... Analysis of 16S-23S rRNA internal transcribed spacer regions in Pasteurellaceae isolated from laboratory rodents ...
more infohttp://www.straininfo.net/strains/53260

BacMapBacMap

Actinobacillus pleuropneumoniae, a Gram-negative, non-motile, facultatively anaerobic Pasteurellaceae, causes porcine ...
more infohttp://bacmap.wishartlab.com/organisms/721

Microbiology Society Journals | Reclassification of Pasteurella gallinarum, [Haemophilus] paragallinarum, Pasteurella avium and...Microbiology Society Journals | Reclassification of Pasteurella gallinarum, [Haemophilus] paragallinarum, Pasteurella avium and...

This group can also be separated by phenotypic testing from all other recognized and named taxa within the Pasteurellaceae. As ... 16S rRNA gene sequences for 11 other taxa within avian 16S rRNA cluster 18 as well as seven other taxa from the Pasteurellaceae ... a major subcluster within the avian 16S rRNA cluster 18 of the family Pasteurellaceae. An extended phenotypic characterization ... Bisgaard, M. ( 1993 ). Ecology and significance of Pasteurellaceae in animals. Zentbl Bakteriol 279, 7-26.[CrossRef] ...
more infohttp://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63357-0

The role of lipooligosaccharide phosphorylcholine in colonization and pathogenesis of Histophilus somni in cattleThe role of lipooligosaccharide phosphorylcholine in colonization and pathogenesis of Histophilus somni in cattle

Histophilus somni is a Gram-negative bacterium and member of the Pasteurellaceae that is responsible for respiratory disease ...
more infohttps://vtechworks.lib.vt.edu/handle/10919/18749

Pasteurella multocida: Definition with Pasteurella multocida Pictures and PhotosPasteurella multocida: Definition with Pasteurella multocida Pictures and Photos

pasteurellaceae. pasteurellaceae infections. pasteurellosis. pasteurisable. pasteurisation. pasteurise. pasteurised. ...
more infohttp://www.lexic.us/definition-of/pasteurella_multocida

PasteurellaceaePasteurellaceae

Family Pasteurellaceae. Warning: In the List of Prokaryotic names with Standing in Nomenclature, an arrow (→) only indicates ... n. Pasteurellaceae, the Pasteurella family. Valid publication: Validation List no. 7. Int. J. Syst. Bacteriol., 1981, 31, 382- ... Pasteurellaceae Pohl 1981, fam. nov. Type genus: ¤ Pasteurella Trevisan 1887 (Approved Lists 1980). Etymology: N.L. fem. n. ... Pasteurellaceae. Inaug. Diss. Phillips-Universität Marburg/Lahn. Mauersberger, Marburg, 1979. Validation List no. 7 in IJSEM ...
more infohttp://www.bacterio.net/pasteurellaceae.html

Insights into Pasteurellaceae carriage dynamics in the nasal passages of healthy beef calves | Scientific ReportsInsights into Pasteurellaceae carriage dynamics in the nasal passages of healthy beef calves | Scientific Reports

Insights into Pasteurellaceae carriage dynamics in the nasal passages of healthy beef calves. *A. C. Thomas. ORCID: orcid.org/ ... Thomas, A.C., Bailey, M., Lee, M.R.F. et al. Insights into Pasteurellaceae carriage dynamics in the nasal passages of healthy ... For isolation of Pasteurellaceae, nasal swabs were thawed on ice, vortexed and a 50 µl STGG broth aliquot was spread over the ... Detection of tetracycline-resistant and susceptible Pasteurellaceae in the nasopharynx of loose group-housed calves. Vet Res ...
more infohttps://www.nature.com/articles/s41598-019-48007-5?error=cookies_not_supported&code=bdb21964-82e6-479d-b6be-3faf2d41126a

Frontiers | Evidence of Illegitimate Recombination Between Two Pasteurellaceae Plasmids Resulting in a Novel Multi-Resistance...Frontiers | Evidence of Illegitimate Recombination Between Two Pasteurellaceae Plasmids Resulting in a Novel Multi-Resistance...

Two are almost identical to plasmids pTetHS016 and pB1001, found in other members of the Pasteurellaceae (i.e., H. parasuis and ... FIGURE 1. Schematic representation of the structure and organization of genes in different Pasteurellaceae tet(B) and multi- ... Replicons which have evolved to be stably maintained in Pasteurellaceae frequently contain resistance genes that appear to have ... Michael, G. B., Bossé, J. T., and Schwarz, S. (2018). Antimicrobial resistance in Pasteurellaceae of veterinary origin. ...
more infohttps://www.frontiersin.org/articles/10.3389/fmicb.2018.02489/full

Evolution of competence and DNA uptake specificity in the Pasteurellaceae | BMC Evolutionary Biology | Full TextEvolution of competence and DNA uptake specificity in the Pasteurellaceae | BMC Evolutionary Biology | Full Text

A phylogeny of the Pasteurellaceae based on 12 protein coding genes from species with sequenced genomes shows two strongly ... We used the distribution of competence genes and DNA uptake specificity in H. influenzaes family, the Pasteurellaceae, to ... Competence and DNA uptake specificity are ancestral properties of the Pasteurellaceae, with divergent USSs and uptake ... In the Pasteurellaceae and Neisseriaceae the molecular basis of this specificity is preferential binding of the uptake ...
more infohttps://bmcevolbiol.biomedcentral.com/articles/10.1186/1471-2148-6-82

Comparative phylogenies of the housekeeping genes atpD, infB and rpoB and the 16S rRNA gene within the Pasteurellaceae. -...Comparative phylogenies of the housekeeping genes atpD, infB and rpoB and the 16S rRNA gene within the Pasteurellaceae. -...

36 and 28 representative taxa of the Pasteurellaceae, respectively. The monophyly of representatives of the genus ... housekeeping gene sequence comparisons resulted in quite different evolutionary scenarios for members of the Pasteurellaceae. ... gene and 16S rRNA gene sequences were compared to improve the classification of the bacterial family Pasteurellaceae and ... Evolution of competence and DNA uptake specificity in the Pasteurellaceae. *Rosemary J Redfield, Wendy A Findlay, Janine Bossé ...
more infohttps://www.semanticscholar.org/paper/Comparative-phylogenies-of-the-housekeeping-genes-Christensen-Kuhnert/f4deceaf918bb189bd1ae9ea1ed4dc2109f2f452

Comparative functional genomic analysis of Pasteurellaceae adhesins using phage display  : Sussex Research OnlineComparative functional genomic analysis of Pasteurellaceae adhesins using phage display : Sussex Research Online

The Pasteurellaceae contain a number of important animal pathogens. Although related, the various members of this family cause ... Surprisingly little is known about the adhesins of the Pasteurellaceae. To attempt to identify the genes coding for adhesins to ... Comparative functional genomic analysis of Pasteurellaceae adhesins using phage display. Veterinary Microbiology, 122 (1-2). pp ...
more infohttp://sro.sussex.ac.uk/43409/

Identification of putative adhesins of Actinobacillus suis and their homologues in other members of the family Pasteurellaceae ...Identification of putative adhesins of Actinobacillus suis and their homologues in other members of the family Pasteurellaceae ...

This work begins to examine adhesin-associated factors that allow some members of the family Pasteurellaceae to invade the ... and to other members of the family Pasteurellaceae that colonise tonsils. At present, very little is known about the genes ... suis share even greater homology with those of other pathogens within the family Pasteurellaceae. Similar to A. suis, these ... Pasteurellaceae, and in other species outside of the family Pasteurellaceae. The fhaC gene is also predicted to encode highly ...
more infohttps://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-015-1659-x

Frontiers | PCR-Based Analysis of ColE1 Plasmids in Clinical Isolates and Metagenomic Samples Reveals Their Importance as Gene...Frontiers | PCR-Based Analysis of ColE1 Plasmids in Clinical Isolates and Metagenomic Samples Reveals Their Importance as Gene...

In Pasteurellaceae, ColE1 plasmids carried almost exclusively antibiotic resistance genes. In Enterobacteriaceae, these ... In Pasteurellaceae, ColE1 plasmids carried almost exclusively antibiotic resistance genes. In Enterobacteriaceae, these ... either specific for Enterobacteriaceae or specific for Pasteurellaceae. To validate this PCR-based system, we tested various ... either specific for Enterobacteriaceae or specific for Pasteurellaceae. To validate this PCR-based system, we tested various ...
more infohttps://www.frontiersin.org/articles/10.3389/fmicb.2018.00469/full

Illumina MiSeq 16S amplicon sequence analysis of bovine respiratory disease associated bacteria in lung and mediastinal lymph...Illumina MiSeq 16S amplicon sequence analysis of bovine respiratory disease associated bacteria in lung and mediastinal lymph...

Leptotrichiaceae, Mycoplasma and Pasteurellaceae showed higher relative abundances in post-mortem lymph node samples collected ... Leptotrichiaceae, Mycoplasma, Pasteurellaceae, and Fusobacterium were the most abundant OTUs identified in the lungs and lymph ... Pasteurellaceae. Pasteurellaceae was one of the most abundant OTUs identified overall and was consistently more abundant in ... Leptotrichiaceae, Mycoplasma, Pasteurellaceae, and Fusobacterium were the most abundant OTUs identified in the lungs and lymph ...
more infohttps://bmcvetres.biomedcentral.com/articles/10.1186/s12917-017-1035-2

Microbiology - Online Flashcards by Steven Centola | BrainscapeMicrobiology - Online Flashcards by Steven Centola | Brainscape

Pasteurellaceae Pasteurellaceae Sample Cards: what kind of bacterial characteristic..., haemophilus, haemophilus virulence ...
more infohttps://www.brainscape.com/packs/microbiology-2720489

ICEApl1, an Integrative Conjugative Element Related to ICEHin1056, Identified in the Pig Pathogen Actinobacillus...ICEApl1, an Integrative Conjugative Element Related to ICEHin1056, Identified in the Pig Pathogen Actinobacillus...

... found in other members of the Pasteurellaceae. ICEApl1 is most similar to the 59393 bp ICEHin1056, from Haemophilus influenzae ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/27379024

Identification of Lonepinella sp. in Koala Bite Wound Infections, Queensland, Australia - Volume 25, Number 1-January 2019 -...Identification of Lonepinella sp. in Koala Bite Wound Infections, Queensland, Australia - Volume 25, Number 1-January 2019 -...

Occurrence of Pasteurellaceae Bacteria in the Oral Cavity of Selected Marsupial Species. J Zoo Wildl Med. 2017;48:1215-8. DOI ... Phylogeny of the family Pasteurellaceae based on rpoB sequences. Int J Syst Evol Microbiol. 2004;54:1393-9. DOIPubMed ... For the rpoB gene sequence, an identity of 85%-88% for genera and 95% for species has been suggested for Pasteurellaceae (2,10, ... Identification of animal Pasteurellaceae by MALDI-TOF mass spectrometry. J Microbiol Methods. 2012;89:1-7. DOIPubMed ...
more infohttps://wwwnc.cdc.gov/eid/article/25/1/17-1359_article

Haemophylus ducreyi by Yamani Pineda on PreziHaemophylus ducreyi by Yamani Pineda on Prezi

Familia: Pasteurellaceae. Género: Haemophilus. Especie: H. ducrey Epidemiología Es endémico en países tropicales y ...
more infohttps://prezi.com/q0jfq2h2mn8w/haemophylus-ducreyi/

Immunogenicity and Safety of Sanofi Pasteur Pentaxim Combined Vaccine in Infants in Thailand - Full Text View - ClinicalTrials...Immunogenicity and Safety of Sanofi Pasteur Pentaxim Combined Vaccine in Infants in Thailand - Full Text View - ClinicalTrials...

The present clinical study will assess the immunogenicity and reactogenicity of Sanofi Pasteurs DTacP-IPV// PRP~T combined vaccine (Pentavac™ or Pentaxim™) as a three-dose primary vaccination at 2, 4, and 6 months of age followed by a booster dose during the second year of life and concomitant hepatitis B vaccine at 2 and 6 months of age in infants in Thailand ...
more infohttps://clinicaltrials.gov/ct2/show/NCT00255021

HOGENOM: ACTP2 1 PE1001HOGENOM: ACTP2 1 PE1001

OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; OC Pasteurellaceae; Actinobacillus. OX NCBI_TaxID=416269; RN ...
more infohttp://pbil.univ-lyon1.fr/cgi-bin/acnuc-search-id?query=ACTP2_1_PE1001&db=HOGENOM&ident=ACNUC7421
  • From this exhaustive computational analysis we developed a new PCR-based technique, targeting a specific sequence in the conserved region, for the screening, capture and sequencing of these small plasmids, either specific for Enterobacteriaceae or specific for Pasteurellaceae. (frontiersin.org)
  • Leptotrichiaceae, Mycoplasma and Pasteurellaceae showed higher relative abundances in post-mortem lymph node samples collected from fatal cases of BRD in dairy calves, compared with clinically healthy calves without lung lesions. (biomedcentral.com)