AnatomyDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsHealth Care
ParaffinParaffin EmbeddingTissue FixationFormaldehydeFixativesImmunoenzyme TechniquesImmunohistochemistryFrozen SectionsMicrotomyStaining and LabelingKeroseneWaxesTissue PreservationPlastic EmbeddingHistocytological Preparation TechniquesMineral OilTissue EmbeddingDecalcification TechniqueTumor Markers, BiologicalPolymerase Chain ReactionBiopsyHematoxylinIn Situ HybridizationNucleolus Organizer RegionAntibodies, MonoclonalPneumonia, LipidOilsDNA, NeoplasmKi-67 AntigenHistocytochemistryNeoplasm ProteinsPalatine TonsilEosine Yellowish-(YS)Breast NeoplasmsPathology, MolecularSilver StainingAdenocarcinomaPloidiesIn Situ Hybridization, FluorescenceTissue Array AnalysisPrognosisTissue BanksAntigens, NeoplasmProliferating Cell Nuclear AntigenMicrowavesAdenofibromaSensitivity and SpecificityCarcinoma, Squamous CellLymph NodesDigoxigeninPeriodic Acid-Schiff ReactionRetrospective StudiesEmulsionsDiathermyHistologyDNA Probes, HPVReceptors, EstrogenKeratinsKeratin-13Lymphoma, T-CellCarcinoma, Ductal, Breast
Paraffin
A mixture of solid hydrocarbons obtained from petroleum. It has a wide range of uses including as a stiffening agent in ointments, as a lubricant, and as a topical anti-inflammatory. It is also commonly used as an embedding material in histology.
Paraffin Embedding
The infiltrating of tissue specimens with paraffin, as a supporting substance, to prepare for sectioning with a microtome.
Tissue Fixation
The technique of using FIXATIVES in the preparation of cytologic, histologic, or pathologic specimens for the purpose of maintaining the existing form and structure of all the constituent elements.
Formaldehyde
A highly reactive aldehyde gas formed by oxidation or incomplete combustion of hydrocarbons. In solution, it has a wide range of uses: in the manufacture of resins and textiles, as a disinfectant, and as a laboratory fixative or preservative. Formaldehyde solution (formalin) is considered a hazardous compound, and its vapor toxic. (From Reynolds, Martindale The Extra Pharmacopoeia, 30th ed, p717)
Fixatives
Agents employed in the preparation of histologic or pathologic specimens for the purpose of maintaining the existing form and structure of all of the constituent elements. Great numbers of different agents are used; some are also decalcifying and hardening agents. They must quickly kill and coagulate living tissue.
Immunoenzyme Techniques
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Immunohistochemistry
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Frozen Sections
Thinly cut sections of frozen tissue specimens prepared with a cryostat or freezing microtome.
Microtomy
The technique of using a microtome to cut thin or ultrathin sections of tissues embedded in a supporting substance. The microtome is an instrument that hold a steel, glass or diamond knife in clamps at an angle to the blocks of prepared tissues, which it cuts in sections of equal thickness.
Staining and Labeling
The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.
Kerosene
A refined petroleum fraction used as a fuel as well as a solvent.
Waxes
A plastic substance deposited by insects or obtained from plants. Waxes are esters of various fatty acids with higher, usually monohydric alcohols. The wax of pharmacy is principally yellow wax (beeswax), the material of which honeycomb is made. It consists chiefly of cerotic acid and myricin and is used in making ointments, cerates, etc. (Dorland, 27th ed)
Tissue Preservation
The process by which a tissue or aggregate of cells is kept alive outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).
Plastic Embedding
The infiltrating of histological specimens with plastics, including acrylic resins, epoxy resins and polyethylene glycol, for support of the tissues in preparation for sectioning with a microtome.
Histocytological Preparation Techniques
Methods of preparing cells or tissues for examination and study of their origin, structure, function, or pathology. The methods include preservation, fixation, sectioning, staining, replica, or other technique to allow for viewing using a microscope.
Mineral Oil
A mixture of liquid hydrocarbons obtained from petroleum. It is used as laxative, lubricant, ointment base, and emollient.
Tissue Embedding
The technique of placing cells or tissue in a supporting medium so that thin sections can be cut using a microtome. The medium can be paraffin wax (PARAFFIN EMBEDDING) or plastics (PLASTIC EMBEDDING) such as epoxy resins.
Decalcification Technique
Removal of minerals from bones during bone examination.
Tumor Markers, Biological
Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Biopsy
Removal and pathologic examination of specimens in the form of small pieces of tissue from the living body.
Hematoxylin
A dye obtained from the heartwood of logwood (Haematoxylon campechianum Linn., Leguminosae) used as a stain in microscopy and in the manufacture of ink.
In Situ Hybridization
A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.
Nucleolus Organizer Region
The chromosome region which is active in nucleolus formation and which functions in the synthesis of ribosomal RNA.
Antibodies, Monoclonal
Antibodies produced by a single clone of cells.
Pneumonia, Lipid
Pneumonia due to aspiration or inhalation of various oily or fatty substances.
Oils
Unctuous combustible substances that are liquid or easily liquefiable on warming, and are soluble in ether but insoluble in water. Such substances, depending on their origin, are classified as animal, mineral, or vegetable oils. Depending on their behavior on heating, they are volatile or fixed. (Dorland, 28th ed)
DNA, Neoplasm
DNA present in neoplastic tissue.
Ki-67 Antigen
A CELL CYCLE and tumor growth marker which can be readily detected using IMMUNOCYTOCHEMISTRY methods. Ki-67 is a nuclear antigen present only in the nuclei of cycling cells.
Histocytochemistry
Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.
Neoplasm Proteins
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
Palatine Tonsil
A round-to-oval mass of lymphoid tissue embedded in the lateral wall of the PHARYNX. There is one on each side of the oropharynx in the fauces between the anterior and posterior pillars of the SOFT PALATE.
Eosine Yellowish-(YS)
A versatile red dye used in cosmetics, pharmaceuticals, textiles, etc., and as tissue stain, vital stain, and counterstain with HEMATOXYLIN. It is also used in special culture media.
Breast Neoplasms
Tumors or cancer of the human BREAST.
Pathology, Molecular
A subspecialty of pathology concerned with the molecular basis (e.g., mutations) of various diseases.
Silver Staining
The use of silver, usually silver nitrate, as a reagent for producing contrast or coloration in tissue specimens.
Adenocarcinoma
A malignant epithelial tumor with a glandular organization.
Ploidies
The degree of replication of the chromosome set in the karyotype.
In Situ Hybridization, Fluorescence
A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.
Tissue Array Analysis
The simultaneous analysis of multiple samples of TISSUES or CELLS from BIOPSY or in vitro culture that have been arranged in an array format on slides or microchips.
Prognosis
A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations.
Tissue Banks
Centers for acquiring, characterizing, and storing organs or tissue for future use.
Antigens, Neoplasm
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Proliferating Cell Nuclear Antigen
Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.
Microwaves
That portion of the electromagnetic spectrum from the UHF (ultrahigh frequency) radio waves and extending into the INFRARED RAYS frequencies.
Adenofibroma
A benign neoplasm composed of glandular and fibrous tissues, with a relatively large proportion of glands. (Stedman, 25th ed)
Sensitivity and Specificity
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
Carcinoma, Squamous Cell
A carcinoma derived from stratified SQUAMOUS EPITHELIAL CELLS. It may also occur in sites where glandular or columnar epithelium is normally present. (From Stedman, 25th ed)
Lymph Nodes
They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.
Digoxigenin
3 beta,12 beta,14-Trihydroxy-5 beta-card-20(22)-enolide. A cardenolide which is the aglycon of digoxin. Can be obtained by hydrolysis of digoxin or from Digitalis orientalis L. and Digitalis lanata Ehrh.
Periodic Acid-Schiff Reaction
A histochemical technique for staining carbohydrates. It is based on PERIODIC ACID oxidation of a substance containing adjacent hydroxyl groups. The resulting aldehydes react with Schiff reagent to form a colored product.
Retrospective Studies
Studies used to test etiologic hypotheses in which inferences about an exposure to putative causal factors are derived from data relating to characteristics of persons under study or to events or experiences in their past. The essential feature is that some of the persons under study have the disease or outcome of interest and their characteristics are compared with those of unaffected persons.
Emulsions
Colloids formed by the combination of two immiscible liquids such as oil and water. Lipid-in-water emulsions are usually liquid, like milk or lotion. Water-in-lipid emulsions tend to be creams. The formation of emulsions may be aided by amphiphatic molecules that surround one component of the system to form MICELLES.
Diathermy
The induction of local hyperthermia by either short radio waves or high-frequency sound waves.
Histology
The study of the structure of various TISSUES of organisms on a microscopic level.
DNA Probes, HPV
DNA probes specific for the identification of human papilloma virus.
Receptors, Estrogen
Cytoplasmic proteins that bind estrogens and migrate to the nucleus where they regulate DNA transcription. Evaluation of the state of estrogen receptors in breast cancer patients has become clinically important.
Keratins
A class of fibrous proteins or scleroproteins that represents the principal constituent of EPIDERMIS; HAIR; NAILS; horny tissues, and the organic matrix of tooth ENAMEL. Two major conformational groups have been characterized, alpha-keratin, whose peptide backbone forms a coiled-coil alpha helical structure consisting of TYPE I KERATIN and a TYPE II KERATIN, and beta-keratin, whose backbone forms a zigzag or pleated sheet structure. alpha-Keratins have been classified into at least 20 subtypes. In addition multiple isoforms of subtypes have been found which may be due to GENE DUPLICATION.
Keratin-13
A type I keratin that is found associated with the KERATIN-4 in the internal stratified EPITHELIUM. Defects in gene for keratin 13 cause HEREDITARY MUCOSAL LEUKOKERATOSIS.
Lymphoma, T-Cell
A group of heterogeneous lymphoid tumors representing malignant transformations of T-lymphocytes.
Carcinoma, Ductal, Breast
An invasive (infiltrating) CARCINOMA of the mammary ductal system (MAMMARY GLANDS) in the human BREAST.

Immunohistochemical analysis of arterial wall cellular infiltration in Buerger's disease (endarteritis obliterans). (1/1288)

PURPOSE: The diagnosis of Buerger's disease has depended on clinical symptoms and angiographic findings, whereas pathologic findings are considered to be of secondary importance. Arteries from patients with Buerger's tissue were analyzed histologically, including immunophenotyping of the infiltrating cells, to elucidate the nature of Buerger's disease as a vasculitis. METHODS: Thirty-three specimens from nine patients, in whom Buerger's disease was diagnosed on the basis of our clinical and angiographic criteria between 1980 and 1995 at Nagoya University Hospital, were studied. Immunohistochemical studies were performed on paraffin-embedded tissue with a labeled streptoavidin-biotin method. RESULTS: The general architecture of vessel walls was well preserved regardless of the stage of disease, and cell infiltration was observed mainly in the thrombus and the intima. Among infiltrating cells, CD3(+) T cells greatly outnumbered CD20(+) B cells. CD68(+) macrophages or S-100(+) dendritic cells were detected, especially in the intima during acute and subacute stages. All cases except one showed infiltration by the human leukocyte antigen-D region (HLA-DR) antigen-bearing macrophages and dendritic cells in the intima. Immunoglobulins G, A, and M (IgG, IgA, IgM) and complement factors 3d and 4c (C3d, C4c) were deposited along the internal elastic lamina. CONCLUSION: Buerger's disease is strictly an endarteritis that is introduced by T-cell mediated cellular immunity and by B-cell mediated humoral immunity associated with activation of macrophages or dendritic cells in the intima.  (+info)

Diagnosis of malignant catarrhal fever by PCR using formalin-fixed, paraffin-embedded tissues. (2/1288)

A previously described polymerase chain reaction (PCR) assay (amplification of a 238-bp fragment of ovine herpesvirus 2 [OHV-2] genomic DNA) for diagnosis of sheep-associated malignant catarrhal fever (MCF) was adapted for use on formalin-fixed, paraffin-embedded tissues. Variables affecting its use were examined. Archived tissues from cattle, white-tailed deer (Odocoileus virginianus), and bison (Bison bison) diagnosed with MCF by clinical signs or histologic lesions were obtained from 2 veterinary diagnostic laboratories. Tissues from healthy animals and from animals diagnosed with other common bovine viral diseases were examined as controls. A total of 86 blocks from 37 suspect MCF cases were examined. Forty-one blocks from healthy animals and animals with unrelated viral diseases were examined as controls. The assay was specific for sheep-associated MCF and did not yield false-positive signals from healthy animals or from cases of infectious bovine rhinotracheitis, bovine virus diarrhea, mucosal disease, or parainfluenza-3 virus infection. A wide variety of tissues were suitable substrates, including spleen, lymph node, intestine, brain, lung, and kidney. Extracted DNA provided a more suitable target than did unextracted tissue lysate. The highest levels of viral DNA were present in lymphoid organs and intestine, but the data indicate that in acute clinical cases, most organs contain sufficient viral DNA to serve as a suitable diagnostic specimen. Fixation of 0.5-cm3 blocks of tissue in 10% neutral buffered formalin was deleterious to the target DNA, and PCR signals progressively diminished after fixation for >45 days. Detection of genomic DNA of OHV-2 by PCR was successful for archived tissues that were 15 years old.  (+info)

Immunocytochemical detection of Candida albicans in formalin fixed, paraffin embedded material. (3/1288)

AIM: To assess the ability of the commercially available monoclonal antibody 1B12 (BioGenex, San Ramon, USA) to identify C albicans in formalin fixed, paraffin wax embedded material (FFPE). METHODS: Broth cultures of 20 strains of seven Candida species were resuspended in 4% agarose blocks, fixed in formalin for 24 hours, and embedded in paraffin wax. In addition, 16 blocks of FFPE tissue known to contain periodic acid-Schiff positive fungal hyphae were examined. Antigen retrieval involved microwave treatment of specimens in citrate buffer (0.01 M; pH 6.5) before addition of 1B12 antibody for 24 hours. Bound antibody was subsequently detected using a biotinylated link antibody and a peroxidase conjugated streptavidin. RESULTS: Only C albicans strains were 1B12 positive in the agarose blocks. All FFPE tissue blocks were found to contain 1B12 positive hyphal structures, indicating the presence of C albicans. CONCLUSIONS: The ability to identify candida organisms penetrating the lesional tissue in cases of chronic hyperplastic candidosis will help to clarify the role of individual Candida spp in this important form of oral candidosis.  (+info)

Detection of translocation t(11;14)(q13;q32) in mantle cell lymphoma by fluorescence in situ hybridization. (4/1288)

To assess an unequivocal diagnosis of mantle cell lymphoma (MCL), we have developed a fluorescence in situ hybridization (FISH) assay, enabling the demonstration of t(11;14)(q13;q32) directly on pathological samples. We have first selected CCND1 and IGH probes encompassing the breakpoint regions on both chromosomes. Then, we have defined experimental conditions enabling us to obtain bright clear-cut signals in all of the samples, independently of the initial fixation conditions. We have analyzed single-cell suspensions from 26 formalin-fixed, paraffin-embedded MCL samples with this set of probes. In all cases, we have found a fusion signal (ie, a t(11;14)(q13;q32) translocation) in 14% to 99% of cells (median, 87%). So far, IGH-CCND1 fusions have been detected in all of the 51 MCL patients that we have analyzed by FISH (either on paraffin-embedded tumor samples or on peripheral blood samples). Regarding the low sensitivity of other techniques used to diagnose t(11;14)(q13;q32) (ie, 70% to 75% for cytogenetics and 50% to 60% for polymerase chain reaction), our FISH assay is by far the most sensitive technique. Moreover, because of the quality of the fluorescent signals and the rapidity of the experiment, this technique is widely applicable, even in routine cytogenetics or pathology laboratories. As MCL patients are usually refractory to standard therapy, an unambiguous diagnosis is needed to propose adapted therapeutic strategies, and this highly sensitive assay may be of great value for accurate diagnosis in difficult cases.  (+info)

Evidence for two candidate tumour suppressor loci on chromosome 9q in transitional cell carcinoma (TCC) of the bladder but no homozygous deletions in bladder tumour cell lines. (5/1288)

The most frequent genetic alterations in transitional cell carcinoma (TCC) of the bladder involve loss of heterozygosity (LOH) on chromosome 9p and 9q. The LOH on chromosome 9p most likely targets the CDKN2 locus, which is inactivated in about 50% of TCCs. Candidate genes that are the target for LOH on chromosome 9q have yet to be identified. To narrow the localization of one or more putative tumour suppressor genes on this chromosome that play a role in TCC of the bladder, we examined 59 tumours with a panel of microsatellite markers along the chromosome. LOH was observed in 26 (44%) tumours. We present evidence for two different loci on the long arm of chromosome 9 where potential tumour suppressor genes are expected. These loci are delineated by interstitial deletions in two bladder tumours. Our results confirm the results of others and contribute to a further reduction of the size of these regions, which we called TCC1 and TCC2. These regions were examined for homozygous deletions with EST and STS markers. No homozygous deletions were observed in 17 different bladder tumour cell lines.  (+info)

Labeled carcinoembryonic antigen antibodies excitable by infrared rays: a novel diagnostic method for micro cancers in the digestive tract. (6/1288)

OBJECT: An indocyanine green derivative (ICG-sulfo-OSu) was used as the labeling substance for monoclonal antibody, and a fluorescence imaging system appropriate for ICG-sulfo-OSu excitable by infrared rays (IR) was developed. The goal of this study was to demonstrate antibody labeling at the tissue level using this new imaging system. MATERIALS AND METHODS: ICG-sulfo-OSu labeled mouse anti-human carcinoembryonic antigen (CEA) monoclonal antibody, a newly developed imaging system, and an infrared ray microscope were employed in this experiment. Paraffin sections of human colon cancer previously proven to have cross-reactivity to anti-CEA antibody were examined. RESULTS: Positive staining was seen as a brownish discoloration of oxidized 3,3'-diaminobenzidine tetrahydrochloride (DAB) in sections that reacted with ICG-sulfo-OSu-labeled anti-CEA antibody, and the fluorescence was well-matched with the oxidized DAB-positive sites. CONCLUSION: Specific antibodies labeled with ICG-sulfo-OSu have significant affinity to cancer cells and seem to reflect sufficient amounts of fluorescence by IR to be useful in a system for the endoscopic detection of micro cancers using the immunohistochemical staining method.  (+info)

Optimisation of DNA and RNA extraction from archival formalin-fixed tissue. (7/1288)

Archival, formalin-fixed, paraffin-embedded tissue is an invaluable resource for molecular genetic studies but the extraction of high quality nucleic acid may be problematic. We have optimised DNA extraction by comparing 10 protocols, including a commercially available kit and a novel method that utilises a thermal cycler. The thermal cycler and Chelex-100 extraction method yielded DNA capable of amplification by PCR from every block and 61% of sections versus 54% using microwave and Chelex-100, 15% with classical xylene-based extraction and 60% of sections using the kit. Successful RNA extraction was observed, by beta-actin amplification, in 83.7% sections for samples treated by the thermal cycler and Chelex-100 method. Thermal cycler and Chelex-100 extraction of nucleic acid is reliable, quick and inexpensive.  (+info)

Detection of clonal B cells in microdissected reactive lymphoproliferations: possible diagnostic pitfalls in PCR analysis of immunoglobulin heavy chain gene rearrangement. (8/1288)

AIMS: To evaluate the specificity of standard and fluorescence based (Genescan) polymerase chain reaction (PCR) immunoglobulin heavy chain (IgH) gene rearrangement analysis in complete and microdissected paraffin wax embedded sections from lymphoid proliferations. METHODS: PCR IgH gene rearrangement analysis of whole sections and microdissected fragments (n = 62) from paraffin wax embedded reactive lymph nodes (n = 6) and tonsils (n = 3). Amplificant analysis used both standard methods and automated high resolution fluorescence based quantification and size determination using GENESCAN software. RESULTS: Whole tissue sections were consistently polyclonal in control experiments. IgH gene amplification was successful in 59 of 62 microdissected fragments; only two of 59 showed a polyclonal rearrangement pattern, the remainder being oligoclonal or monoclonal. Reanalysis was possible in 33 samples; six showed reproducible bands on gel analysis and satisfied accepted criteria for monoclonality. Use of high resolution gels with Genescan analysis improved sensitivity and band definition; however, three samples still appeared to be monoclonal. CONCLUSIONS: These results confirm that PCR based IgH gene rearrangement analysis is a sensitive and specific method for demonstrating B cell clonality in whole paraffin wax embedded sections. However, oligoclonal and monoclonal rearrangement patterns are regularly encountered in small tissue fragments from otherwise unremarkable reactive lymphoproliferations, possibly because of preferential priming or detection of local B cell clones. Data from clonal analysis of small, microdissected or lymphocyte poor samples must be evaluated critically. It is recommended that analyses should be run in parallel on at least two tissue specimens. Only reproducible bands present in more than one sample should be considered to be suggestive of neoplasia.  (+info)

Comparative investigations of T cell receptor γ gene rearrangements in frozen and formalin-fixed paraffin wax-embedded tissues...Comparative investigations of T cell receptor γ gene rearrangements in frozen and formalin-fixed paraffin wax-embedded tissues...
Aim: To compare clonal T cell receptor γ (TCRγ) gene rearrangements in frozen and formalin-fixed paraffin wax-embedded (FFPE) tissue, using capillary electrophoresis for use in diagnostics, as T cell lymphomas may be difficult to diagnose by conventional methods.. Methods: The DNA for PCR was extracted from frozen and FFPE tissue, cell lines and blood. PCR primers Vγ1-8, Vγ9, Vγ10 or Vγ11 (5′ end labelled) combined with a mixture of JγP1/JγP/JγP2/Jγ2 (unlabelled) were used. Monoclonal cases were sequenced and clonality, reproducibility, sensitivity and specificity analyses were carried out.. Results: In all cases the molecular test was found to be in agreement with the histological diagnosis. Discrepancies were found between frozen and FFPE tissue in 18 of 56 (32%) tests. The method was highly reproducible. The sensitivity was found to be 0.5% for cell lines and 1% for patient specimens and the specificity 100%. The junctional region between the Vγ and Jγ segments was specific for ...
http://jcp.bmj.com/content/59/6/645
Fluorescence in situ hybridisation analysis of formalin-fixed paraffin-embedded tissue sections in the diagnostic work-up of...Fluorescence in situ hybridisation analysis of formalin-fixed paraffin-embedded tissue sections in the diagnostic work-up of...
Aims In recent years the genetic aberrations associated with diffuse large B-cell lymphoma and the new subtype described in the 2008 revision of the WHO classification, B-cell lymphoma, unclassifiable, with features intermediate between diffuse large B-cell lymphoma and Burkitt lymphoma have been increasingly well defined. Recurrent genetic abnormalities include rearrangements involving MYC (8q24), BCL2 (18q21) and BCL6 (3q27); as the prognostic and therapeutic implications associated with these abnormalities are clarified their accurate identification at diagnosis is becoming increasingly critical. We describe our experience of using a panel of fluorescence in situ hybridisation (FISH) probes on formalin-fixed paraffin-embedded tissue sections in the diagnostic work-up of 162 patients with non-Burkitt high grade B-cell non-Hodgkins lymphomas (HG-BNHL). ...
http://jcp.bmj.com/content/64/9/802
Performance comparison of two commercial human whole-exome capture systems on formalin-fixed paraffin-embedded lung...
TY - JOUR. T1 - Performance comparison of two commercial human whole-exome capture systems on formalin-fixed paraffin-embedded lung adenocarcinoma samples. AU - Bonfiglio, Silvia. AU - Vanni, Irene. AU - Rossella, Valeria. AU - Truini, Anna. AU - Lazarevic, Dejan. AU - Dal Bello, Maria Giovanna. AU - Alama, Angela. AU - Mora, Marco. AU - Rijavec, Erika. AU - Genova, Carlo. AU - Cittaro, Davide. AU - Grossi, Francesco. AU - Coco, Simona. PY - 2016/8/30. Y1 - 2016/8/30. N2 - Background: Next Generation Sequencing (NGS) has become a valuable tool for molecular landscape characterization of cancer genomes, leading to a better understanding of tumor onset and progression, and opening new avenues in translational oncology. Formalin-fixed paraffin-embedded (FFPE) tissue is the method of choice for storage of clinical samples, however low quality of FFPE genomic DNA (gDNA) can limit its use for downstream applications. Methods: To investigate the FFPE specimen suitability for NGS analysis and to ...
https://moh-it.pure.elsevier.com/en/publications/performance-comparison-of-two-commercial-human-whole-exome-captur-2
Validation of a robust proteomic analysis carried out on formalin-fixed paraffin-embedded tissues of the pancreas obtained from...Validation of a robust proteomic analysis carried out on formalin-fixed paraffin-embedded tissues of the pancreas obtained from...
A number of reports have recently emerged with focus on extraction of proteins from formalin-fixed paraffin-embedded (FFPE) tissues for MS analysis; however, reproducibility and robustness as compared to flash frozen controls is generally overlooked. The goal of this study was to identify and validate a practical and highly robust approach for the proteomics analysis of FFPE tissues. FFPE and matched frozen pancreatic tissues obtained from mice (n = 8) were analyzed using 1D-nanoLC-MS(MS)2 following work up with commercially available kits. The chosen approach for FFPE tissues was found to be highly comparable to that of frozen. In addition, the total number of unique peptides identified between the two groups was highly similar, with 958 identified for FFPE and 1070 identified for frozen, with protein identifications that corresponded by approximately 80%. This approach was then applied to archived human FFPE pancreatic cancer specimens (n = 11) as compared to uninvolved tissues (n = 8), where ...
https://scholars.uab.edu/display/pub744044
IJMS | Free Full-Text | Exome Enrichment and SOLiD Sequencing of Formalin Fixed Paraffin Embedded (FFPE) Prostate Cancer...IJMS | Free Full-Text | Exome Enrichment and SOLiD Sequencing of Formalin Fixed Paraffin Embedded (FFPE) Prostate Cancer...
Next generation sequencing (NGS) technologies have revolutionized cancer research allowing the comprehensive study of cancer using high throughput deep sequencing methodologies. These methods detect genomic alterations, nucleotide substitutions, insertions, deletions and copy number alterations. SOLiD (Sequencing by Oligonucleotide Ligation and Detection, Life Technologies) is a promising technology generating billions of 50 bp sequencing reads. This robust technique, successfully applied in gene identification, might be helpful in detecting novel genes associated with cancer initiation and progression using formalin fixed paraffin embedded (FFPE) tissue. This studys aim was to compare the validity of whole exome sequencing of fresh-frozen vs. FFPE tumor tissue by normalization to normal prostatic FFPE tissue, obtained from the same patient. One primary fresh-frozen sample, corresponding FFPE prostate cancer sample and matched adjacent normal prostatic tissue was subjected to exome sequencing. The
http://www.mdpi.com/1422-0067/13/7/8933/htm
IJMS | Free Full-Text | Exome Enrichment and SOLiD Sequencing of Formalin Fixed Paraffin Embedded (FFPE) Prostate Cancer...IJMS | Free Full-Text | Exome Enrichment and SOLiD Sequencing of Formalin Fixed Paraffin Embedded (FFPE) Prostate Cancer...
Next generation sequencing (NGS) technologies have revolutionized cancer research allowing the comprehensive study of cancer using high throughput deep sequencing methodologies. These methods detect genomic alterations, nucleotide substitutions, insertions, deletions and copy number alterations. SOLiD (Sequencing by Oligonucleotide Ligation and Detection, Life Technologies) is a promising technology generating billions of 50 bp sequencing reads. This robust technique, successfully applied in gene identification, might be helpful in detecting novel genes associated with cancer initiation and progression using formalin fixed paraffin embedded (FFPE) tissue. This studys aim was to compare the validity of whole exome sequencing of fresh-frozen vs. FFPE tumor tissue by normalization to normal prostatic FFPE tissue, obtained from the same patient. One primary fresh-frozen sample, corresponding FFPE prostate cancer sample and matched adjacent normal prostatic tissue was subjected to exome sequencing. The
http://www.mdpi.com/1422-0067/13/7/8933/notes
FISH images of formalin fixed paraffin embedded section | Open-iFISH images of formalin fixed paraffin embedded section | Open-i
FISH images of formalin fixed paraffin embedded sections of BAC. Red signals (white arrow heads) are indicative of the p53 gene probe, while green signals (yell
https://openi.nlm.nih.gov/detailedresult.php?img=PMC4307227_13039_2014_104_Fig3_HTML&req=4
In situ hybridization: Detecting viral nucleic acid in formalin-fixed, paraffin-embedded tissue samples<...
TY - JOUR. T1 - In situ hybridization. T2 - Detecting viral nucleic acid in formalin-fixed, paraffin-embedded tissue samples. AU - Mabruk, M. J E M F. PY - 2004/9. Y1 - 2004/9. N2 - In situ hybridization is a method for detecting specific nucleic acid sequences within individual cells. This technique permits visualization of viral nucleic acid or gene expression in individual cells within their histologic context. In situ hybridization is based on the complementary binding of a labeled nucleic acid probe to complementary sequences in cells or tissue sections, followed by visualization of target sequences within the cells. It has been used widely for the detection of viral nucleic acid sequences within individual cells. This review will define the technical approaches of in situ hybridization and its current application to detect viral nucleic acids within formalin-fixed, paraffin-embedded tissue samples, with special reference to the Epstein-Barr virus.. AB - In situ hybridization is a method ...
https://squ.pure.elsevier.com/en/publications/in-situ-hybridization-detecting-viral-nucleic-acid-in-formalin-fi
Cancer Research, Formalin-Fixed Paraffin-Embedded Tissues - Beckman CoulterCancer Research, Formalin-Fixed Paraffin-Embedded Tissues - Beckman Coulter
This video covers off on the challenging samples in cancer research, specifically formalin-fixed paraffin-embedded tissues. Presented by Beckman Coulter Life Sciences.
https://www.beckman.com.au/resources/videos/scientific/challenging-samples-in-cancer-research
Plos One] Tissue lithography: Microscale dewaxing to enable retrospective studies on formalin-fixed
Abstract: We present a new concept, termed tissue lithography (TL), and its implementation which enables retrospective studies on formalin-fixed paraffin-embedded tissue sections. Tissue lithography uses a microfluidic probe to remove microscale areas of the paraffin layer on formalin-fixed paraffin-embedded biopsy sam
https://www.microfluidics-news.com/post/2017/05/15/plos-one-tissue-lithography-microscale-dewaxing-to-enable-retrospective-studies-on-formal
Improved RNA quality and TaqMan ® Pre-amplification method (PreAmp) to enhance expression analysis from formalin fixed paraffin...Improved RNA quality and TaqMan ® Pre-amplification method (PreAmp) to enhance expression analysis from formalin fixed paraffin...
Archival formalin-fixed paraffin-embedded (FFPE) tissues represent an abundant source of clinical specimens; however their use is limited in applications involving analysis of gene expression due to RNA degradation and modification during fixation and processing. This study improved the quality of RNA extracted from FFPE by introducing a heating step into the selected extraction protocols. Further, it evaluated a novel pre-amplification system (PreAmp) designed to enhance expression analysis from tissue samples using assays with a range of amplicon size (62-164 bp). Results from the Bioanalyzer and TaqMan® data showed improvement of RNA quality extracted using the modified protocols from FFPE. Incubation at 70°C for 20 minutes was determined to be the best condition of those tested to disrupt cross-links while not compromising RNA integrity. TaqMan® detection was influenced by master mix, amplicon size and the incorporation of a pre-amplification step. TaqMan® PreAmp consistently achieved decreased
https://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-8-10
Similar papers for KLC1-ALK: A Novel Fusion in Lung Cancer Identified Using a Formalin-Fixed Paraffin-Embedded Tissue Only -...Similar papers for KLC1-ALK: A Novel Fusion in Lung Cancer Identified Using a Formalin-Fixed Paraffin-Embedded Tissue Only -...
The promising results of anaplastic lymphoma kinase (ALK) inhibitors have changed the significance of ALK fusions in several types of cancer. These fusions are no longer mere research targets or diagnostic markers, but they are now directly linked to the therapeutic benefit of patients. However, most available tumor tissues in clinical settings are formalin-fixed and paraffin-embedded (FFPE), and this significantly limits detailed genetic studies in many clinical cases. Although recent technical improvements have allowed the analysis of some known mutations in FFPE tissues, identifying unknown fusion genes by using only FFPE tissues remains difficult. We developed a 5-rapid amplification of cDNA ends-based system optimized for FFPE tissues and evaluated this system on a lung cancer tissue with ALK rearrangement and without the 2 known ALK fusions EML4-ALK and KIF5B-ALK. With this system, we successfully identified a novel ALK fusion, KLC1-ALK. The result was confirmed by reverse transcription
https://www.semanticscholar.org/paper/KLC1-ALK-A-Novel-Fusion-in-Lung-Cancer-Identified-Togashi-Soda/e7e81ace6a7c6b7cfbaa90f45f45554d8fcb1cf4?tab=relatedPapers
Bruker and 3M Expand Exclusive License for Processing of FFPE Tissue Sections to Routine Pathology | BrukerBruker and 3M Expand Exclusive License for Processing of FFPE Tissue Sections to Routine Pathology | Bruker
COLOGNE, Germany - September 25, 2016: At the 28th Congress of the European Society of Pathology, Bruker today announces that it has expanded its license agreement with 3M for processing of FFPE tissue sections. Mass spectrometric imaging by MALDI-TOF (MALDI Imaging) is a technology with great potential for many applications in medicine. It is the leading mass spectrometric method for the analysis of tissues, such as Formalin Fixed Paraffin Embedded (FFPE) tissues. Bruker´s MALDI Tissuetyper™ solution makes this technology broadly applicable due to the unprecedented 10 kHz laser speed, robustness and ease of use of the underlying rapifleX™ MALDI-TOF mass spectrometer. The MALDI Tissuetyper enables anatomical pathology research based on multiplexed proteomic profiles and the spatial distribution of proteomic biomarkers on FFPE tissue sections. The MALDI Tissuetyper is used for the direct, label-free analysis of a wide range of analytes directly from tissue sections. It provides complementary ...
https://www.bruker.com/fr/news-records/single-view/article/bruker-and-3m-expand-exclusive-license-for-processing-of-ffpe-tissue-sections-to-routine-pathology.html
Fresh Frozen Tissue Collection and Storage at Geneticist Inc - MicronicFresh Frozen Tissue Collection and Storage at Geneticist Inc - Micronic
The storage and collection of fresh frozen tissue is by no means an easy task. Tissue sample collection and storage must conform to strict industry
https://micronic.com/blog-posts/fresh-frozen-tissue-collection-and-storage-at-geneticist-inc/
Optimal reference genes for normalization of qRT-PCR data from archival formalin-fixed, paraffin-embedded breast tumors...Optimal reference genes for normalization of qRT-PCR data from archival formalin-fixed, paraffin-embedded breast tumors...
Optimal reference genes for normalization of qRT-PCR data from archival formalin-fixed, paraffin-embedded breast tumors controlling for tumor cell content and decay of ...
http://www.forskningsdatabasen.dk/catalog/244299319
Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction | ProtocolPreparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction | Protocol
This modified extraction protocol improves RNA and DNA yields from more precisely targeted regions of interest in histopathologic...
https://www.jove.com/video/54299/preparation-formalin-fixed-paraffin-embedded-tissue-cores-for-both
Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: a trade off between quantity and...Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: a trade off between quantity and...
Advanced genomic techniques such as Next-Generation-Sequencing (NGS) and gene expression profiling, including NanoString, are vital for the development of personalised medicines, as they enable molecular disease classification. This has become increasingly important in the treatment of cancer, aiding patient selection. However, it requires efficient nucleic acid extraction often from formalin-fixed paraffin-embedded tissue (FFPE). Here we provide a comparison of several commercially available manual and automated methods for DNA and/or RNA extraction from FFPE cancer cell line samples from Qiagen, life Technologies and Promega. Differing extraction geometric mean yields were evaluated across each of the kits tested, assessing dual DNA/RNA extraction vs. specialised single extraction, manual silica column based extraction techniques vs. automated magnetic bead based methods along with a comparison of subsequent nucleic acid purity methods, providing a full evaluation of nucleic acids isolated. Out of the
https://bmcclinpathol.biomedcentral.com/articles/10.1186/s12907-016-0039-3/figures/6
High recovery FASP applied to the proteomic analysis of microdissected formalin fixed paraffin embedded cancer tissues...High recovery FASP applied to the proteomic analysis of microdissected formalin fixed paraffin embedded cancer tissues...
Proteomic analysis of samples isolated by laser capture microdissection from clinical specimens requires sample preparation and fractionation methods suitable for small amounts of protein. Here we describe a streamlined filter-aided sample preparation (FASP) workflow that allows efficient analysis o …
https://pubmed.ncbi.nlm.nih.gov/21526778/?dopt=Abstract
MicroRNA expression profiling outperforms mRNA expression profiling in formalin-fixed paraffin-embedded tissues. - PubMed -...MicroRNA expression profiling outperforms mRNA expression profiling in formalin-fixed paraffin-embedded tissues. - PubMed -...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
https://www.ncbi.nlm.nih.gov/pubmed/19636399?dopt=Abstract
Estimation of age-related DNA degradation from formalin-fixed and paraffin-embedded tissue according to the extraction methodsEstimation of age-related DNA degradation from formalin-fixed and paraffin-embedded tissue according to the extraction methods
Techniques for the extraction and use of nucleic acids from formalin‑fixed and paraffin-embedded (FFPE) tissues, preserved over long time periods in libraries, have been developed. However, DNA extracted from FFPE tissues is generally damaged, and long‑term storage may affect DNA quality. Therefore, it is important to elucidate the effect of long‑term storage on FFPE tissues and evaluate the techniques used to extract DNA from them. In the present study, the yield, purity, and integrity of DNA in FFPE tissue samples was evaluated. Two DNA extraction techniques were used: A silica‑binding DNA collection method using QIAamp DNA FFPE Tissue kit (QIA) and a total tissue DNA collection method using a WaxFree DNA extraction kit (WAX). A total of 25 FFPE tissues from lung adenocarcinomas were studied, which had been surgically resected and fixed at Okayama University Hospital prior to examination and subsequent storage at room temperature for 0.5, 3, 6, 9 and 12 years. Extracted DNA was ...
https://spandidos-publications.com/10.3892/etm.2017.4797
An Optimized Method of Metabolite Extraction from Formalin-Fixed Paraffin-Embedded Tissue for GC/MS Analysis. | Sigma-AldrichAn Optimized Method of Metabolite Extraction from Formalin-Fixed Paraffin-Embedded Tissue for GC/MS Analysis. | Sigma-Aldrich
Sigma-Aldrich offers abstracts and full-text articles by [Anna Wojakowska, Łukasz Marczak, Karol Jelonek, Krzysztof Polanski, Piotr Widlak, Monika Pietrowska].
https://www.sigmaaldrich.com/catalog/papers/26348873
Re: Myoglobin
Hi colleague I use Dako polyclonal Myoglobin A0324 on formalin-fixed paraffin-embedded tissue. Dilution 1:1000 using Antibody-diluent Dako S2022 30 min. Link and label Dako LSAB K5001 each 25 min, chromogen DAB 10 min. Good luck Liv Liv Gr ntoft Departement of Forensic Medicin Universitets sjukhuset 581 85 Link ping Sweden phone +46 13 36 42 58 ...
http://www.histosearch.com/histonet/Nov00A/Re.Myoglobin.html
Formalin-fixed paraffin-embedded clinical tissues show spurious copy n by Elaine A. Mc Sherry, Aloysius Mc Goldrick et al.Formalin-fixed paraffin-embedded clinical tissues show spurious copy n by Elaine A. Mc Sherry, Aloysius Mc Goldrick et al.
Formalin-fixed paraffin-embedded (FFPE) archival clinical specimens are invaluable in discovery of prognostic and therapeutic targets for diseases such as cancer. However, the suitability of FFPE-derived genetic material for array-based comparative genomic hybridization (array-CGH) studies is underexplored. In this study, genetic profiles of matched FFPE and fresh-frozen specimens were examined to investigate DNA integrity differences between these sample types and determine the impact this may have on genetic profiles. Genomic DNA was extracted from three patient-matched FFPE and fresh-frozen clinical tissue samples. T47D breast cancer control cells were also grown in culture and processed to yield a fresh T47D sample, a fresh-frozen T47D sample and a FFPE T47D sample. DNA was extracted from all the samples; array-CGH conducted and genetic profiles of matched samples were then compared. A loss of high molecular weight DNA was observed in the FFPE clinical tissues and FFPE T47D samples. A dramatic
https://epubs.rcsi.ie/pathart/1/
Prevalence of Prognostic Biomarkers in Archival Specimens and Breast Cancer Survival Among White, Black, and Asian Women.
This study addresses two questions: (1) how does prevalence of prognostic biomarkers in old, archival paraffin-embedded tumor biopsy specimens obtained from 50 Asian, 50 black, and 50 white women diagnosed with breast cancer in Oakland, CCA between 1966 and 1990 compare across racial/ethnic groups and to that observed among recent paraffin-embedded specimens, and (2) what is the relationship of these biomarkers to survival, controlling for other biological and socio economic risk factors that affect survival? In Year 1, we have, as planned: (a) abstracted medical chart data on tumor characteristics and treatment for all 150 women, (b) appended these data to an existing database with the women? 5 sociodemographic and reproductive characteristics, (c) determined their vital status as of December 31, 1994, (d) located tumor blocks for 135 of these women, and (e) measured, by immunohistochemistry/image analysis, the following prognostic biomarkers: estrogen, progesterone, androgen, and epidermal growth
http://oai.dtic.mil/oai/oai?verb=getRecord&metadataPrefix=html&identifier=ADA302433
Patent US20060019332 - Deparaffinization compositions for dewaxing tissue specimens - Google PatentsPatent US20060019332 - Deparaffinization compositions for dewaxing tissue specimens - Google Patents
Compositions and methods are provided for dewaxing wax-embedded biological specimens prior to histochemical analysis. The compositions and methods provided can effectively remove wax or improved wax-based embedding materials, particularly paraffin-based, from specimens during preparation for histochemical or other diagnostic analyses, while minimizing danger to users, achieving compatibility with automated use, and maintaining compatibility with downstream histochemical analyses, particularly immunostaining. Compositions of the invention comprise a paraffin-solubilizing organic solvent, a polar organic solvent, and a surfactant. Compositions can further comprise water. The method involves contacting a wax-embedded specimen with the dewaxing composition to solubilize the wax impregnating the specimen prior to histochemical analysis. The method can comprise the further step of washing the dewaxed specimen immediately after dewaxing with an aqueous wash composition comprising a detergent to remove residual
http://www.google.com/patents/US20060019332?dq=No.+6,411,949
NGS-resources: Fwd: NanoStringNorm: an extensible R package for the pre-processing of NanoString mRNA and miRNA dataNGS-resources: Fwd: NanoStringNorm: an extensible R package for the pre-processing of NanoString mRNA and miRNA data
NanoStringNorm: an extensible R package for the pre-processing of NanoString mRNA and miRNA data: Motivation: The NanoString nCounter Platform is a new and promising technology for measuring nucleic acid abundances. It has several advantages over PCR-based techniques, including avoidance of amplification, direct sequence interrogation and digital detection for absolute quantification. These features minimize aspects of experimental error and hold promise for dealing with challenging experimental conditions such as archival formalin-fixed paraffin-embedded samples. However, systematic inter-sample technical artifacts caused by variability in sample preservation, bio-molecular extraction and platform fluctuations must be removed to ensure robust data.. Results: To facilitate this process and to address these issues for NanoString datasets, we have written a pre-processing package called NanoStringNorm in the R statistical language. Key features include an extensible environment for method ...
http://ngs-resources.blogspot.com/2012/05/fwd-nanostringnorm-extensible-r-package.html
C13orf18 and C1Orf166 (MULAN) DNA genes methylation are not associated with cervical cancer and precancerous lesions of human...
Background: Nowadays, molecular biomarkers have critical roles for cancer diagnosis and prognosis in clinical laboratories. Human papillomaviruses are the main agents for etiology of cervical carcinoma. The present survey was conducted to evaluate the genes methylation in cervical cancer and precancerous lesions involvement with HPV genotypes. Materials and Methods: C13orf18 and C1orf166 (MUL1 or Mulan) DNA methylation as potential biomarkers and risk factors was investigated in 112 liquid based cytology and Formalin-Fixed Paraffin-Embedded tissue specimens in Iranian females with cervical intraepithelial neoplasia and dysplasia. Results: In this survey, HPV18 (61.6%) and HPV16 (42.9%) proved to be the most common HPV genotypes identified by In-House Multiplex Real Time PCR. There were no significant relationship between HPV positivity and the methylated DNA genes mentioned above (p , 0.05). Conclusions: Our MethyLight data demonstrated that these genes could not be considered as specific, ...
http://dsp.sbmu.ac.ir/handle/123456789/66758
Plus itPlus it
Background: Mucins are the major secreted glycoproteins of the gastrointestinal tract including the colorectum. Aberrant expression of MUC4 has been observed in adenocarcinomas of pancreas, gallbladder, lungs, salivary glands, and epithelial ovarian carcinomas. However, the staining patterns of MUC4 and their clinical significance in colorectal adenocarcinomas (CRCs) have not been studied. Methods: One hundred and thirty two CRC patients, who underwent surgical resection and had not received any pre- or -post surgery therapy, were selected for this study. Formalin-fixed paraffin-embedded tissue specimens from these patients were evaluated for phenotypic expression of MUC4 using anti-human MUC4 rabbit polyclonal antibody and the horse-radish peroxidase detection method. The expression levels were correlated with key clinicopathologic features and patient outcomes. Results: MUC4 was localized to the cytoplasm in the normal colonic epithelium as well as in CRCs. Based on the cytoplasmic expression, ...
http://cancerres.aacrjournals.org/content/69/9_Supplement/2726
Dysferlin (DYSF) Full Gene Sequence with Interpretation, Whole Blood
Adult Minimum: 6 mL whole blood in ONE pink top tube or TWO lavender top (EDTA) tubes. Children Minimum: 3 mL whole blood in lavender top (EDTA) tube. Optional specimen type: Formalin Fixed paraffin embedded tissue; Fresh Frozen tissue. Testing on smaller volumes than those requested will be attempted. However, in some cases, small blood volumes may compromise the ability to perform testing ...
https://www.healthcare.uiowa.edu/path_handbook/handbook/test3153.html
Unlocking the transcriptomic potential of formalin-fixed paraffin embedded clinical tissues: comparison of gene expression...Unlocking the transcriptomic potential of formalin-fixed paraffin embedded clinical tissues: comparison of gene expression...
High-throughput transcriptomics has matured into a very well established and widely utilised research tool over the last two decades. Clinical datasets generated on a range of different platforms continue to be deposited in public repositories provide an ever-growing, valuable resource for reanalysis. Cost and tissue availability normally preclude processing samples across multiple technologies, making it challenging to directly evaluate performance and whether data from different platforms can be reliably compared or integrated. This study describes our experiences of nine new and established mRNA profiling techniques including Lexogen QuantSeq, Qiagen QiaSeq, BioSpyder TempO-Seq, Ion AmpliSeq, Nanostring, Affymetrix Clariom S or U133A, Illumina BeadChip and RNA-seq of formalin-fixed paraffin embedded (FFPE) and fresh frozen (FF) sequential patient-matched breast tumour samples. The number of genes represented and reliability varied between the platforms, but overall all methods provided data which
https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-020-3365-5
SEQR RNA Amplification | China-Mainland | Sigma-AldrichSEQR RNA Amplification | China-Mainland | Sigma-Aldrich
The SeqPlex RNA Amplification Kit for whole genome transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) of extremely small quantities of degraded/highly fragmented RNA including non-polyA-tailed RNA. The yields from serum, single cells, RNA immunoprecipitation (RIP) or formalin-fixed paraffin-embedded tissue samples (FFPE) are often less than required for successful NGS library preparation. The SeqPlex kit allows the user to pre-amplify these RNA samples while maintaining patterns of differential expression found in the unamplified sample, prior to entering an NGS workflow.. This kit is an extension of the WTA product line and has been developed to integrate with the Illumina® (GAIIx sequencer), SOLiD™ System (from Life Technologies), Ion Torrent (from Life Technologies) and other next generation sequencing workflows.. Features and Benefits. ...
https://www.sigmaaldrich.com/china-mainland/zh/life-science/molecular-biology/whole-genome-amplification/seqr-rna-amplification.html
Molecules and CellsMolecules and Cells
promoter differs between paraffin-fixed and fresh-frozen tissues of glioblastoma (Hamilton et al., 2011). On evaluating 38 patients with GC, LINE-1 was reportedly more hypermethylated in FFPE tissues than in paired fresh-frozen tissues (Song et al., 2016).. Since the patient tissues were dissected for different purposes, they were preserved in various forms and given different pre-treatments. For pathological examination, GC tissues were fixed with formalin and embedded in paraffin. In contrast, for genomic analysis, tissues were immediately frozen in liquid nitrogen without fixation following gastrectomy. Bisulfite conversion can be affected by formalin fixation, as sequence artifacts such as cytosine deamination can be caused by formaldehyde (Do and Dobrovic, 2015). Therefore, we screened the LINE-1 methylation patterns in each tissue type separately to determine whether LINE-1 can be used as a marker under all circumstances. In the current study, we used samples from different patient groups, ...
http://molcells.inforang.com/journal/view.html?doi=10.14348/molcells.2017.0013
RE: CD31?
Hello Jamie, A friend of mine stained xenographs with an anti-VEGF that recognizes both mouse and human VEGF. She purchased this antibody from Oncogene. Sorry it took so long, but my friend returned to Germany and it was difficult to get the information. Best Regards, Matthew Ogdie InnoGenex (925)543-1414 -----Original Message----- From: Jamie Erickson [mailto:[email protected]] Sent: Friday, December 03, 1999 8:10 AM To: [email protected]; [email protected] Subject: Re: CD31? Hi Denise, Im presently working on a mouse model of angiogenisis and I have been asked to try a variety of endothelial markers. I have been using vWF (von Willibrands factor), CD31 and v-cam-1 and I-cam-1. vWF stains endothelial cells very well in formalin fixed paraffin embedded mouse skin tissue. CD31 works on paraffin tissues but what I think the researcher need is a marker for neovascularization, something like a mouse VEGF which I dont know of any? So far I have not been able to shead light on the ...
http://www.histosearch.com/histonet/Dec99/RE.CD31A.html
Isolation and Purification of DNA from FFPE SamplesIsolation and Purification of DNA from FFPE Samples
Isolation and Purification of DNA from FFPE SamplesIsolation and Purification of DNA from FFPE Samples
Quantitation of Steroid Hormones in Thin Fresh Frozen Tissue SectionsQuantitation of Steroid Hormones in Thin Fresh Frozen Tissue Sections
Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...
https://www.infona.pl/resource/bwmeta1.element.acs-doi-10_1021_ac801402a
Sensitivity of HER-2/neu Antibodies in Archival Tissue Samples: Potential Source of Error in Immunohistochemical Studies of...Sensitivity of HER-2/neu Antibodies in Archival Tissue Samples: Potential Source of Error in Immunohistochemical Studies of...
HER-2/neu oncogene amplification and overexpression of breast cancer tissue has been correlated with poor prognosis in women with both node-positive and node-negative disease. However, several studies have not confirmed this association. Review of these studies reveals the presence of considerable methodological variability including differences in study size, follow-up time, techniques and reagents. The majority of papers with clinical follow-up information are immunohistochemical studies using archival, paraffin-embedded breast cancers, and a variety of HER-2/neu antibodies have been used in these studies. Very little information, however, is available about the ability of the antibodies to detect overexpression following tissue processing for paraffin-embedding. Therefore, a series of antibodies, reported in the literature or commercially available, were evaluated to assess their sensitivity and specificity as immunohistochemical reagents. Paraffin-embedded samples of 187 breast cancers, ...
https://cancerres.aacrjournals.org/content/54/10/2771?ijkey=6d32f226b6c4991a22381185dfc3d5a870bbeac9&keytype2=tf_ipsecsha
Tissue Array | Tissue MicroArray | Pantomics inc.Tissue Array | Tissue MicroArray | Pantomics inc.
Our tissue repository contains thousands of paraffin tissue blocks, a large selection of tissue section slides of human cancer, normal tissue and rhesus normal fresh frozen tissue as well as paraffin blocks and slides. Tissue arrays (also Tissue MicroArrays or TMAs) contain up to 1000 tissue samples per microscope slide, allowing high-throughput analysis of RNA, DNA or protein molecules in diseases, which is particularly useful in biomarker research and drug target validation.
https://www.pantomics.com/orders
DiVA - Search resultDiVA - Search result
Background: To improve current treatment strategies for patients with aggressive colorectal cancer (CRC), the molecular understanding of subgroups of CRC with poor prognosis is of vast importance. SOX2 positive tumors have been associated with a poor patient outcome, but the functional role of SOX2 in CRC patient prognosis is still unclear. Methods: An in vitro cell culture model expressing SOX2 was used to investigate the functional role of SOX2 in CRC. In vitro findings were verified using RNA from fresh frozen tumor tissue or immunohistochemistry on formalin fixed paraffin embedded (FFPE) tumor tissue from a cohort of 445 CRC patients. Results: Using our in vitro model, we found that SOX2 expressing cells displayed several characteristics of cancer stem cells; such as a decreased proliferative rate, a spheroid growth pattern, and increased expression of stem cell markers CD24 and CD44. Cells expressing SOX2 also showed down-regulated expression of the intestinal epithelial marker CDX2. We ...
http://umu.diva-portal.org/smash/resultList.jsf?af=%5B%5D&aq=%5B%5B%7B%22personId%22%3A%22authority-person%3A62962%22%7D%5D%5D&aqe=%5B%5D&aq2=%5B%5B%5D%5D&language=en&query=
DNA FFPE Tissue Kit Print - DNA FFPE Tissue Kit Print Exporter, Importer, Manufacturer, Distributor, Supplier, Trading Company,...
SR GROUP - Exporter, Importer, Manufacturer, Distributor, Supplier, Trading Company of DNA FFPE Tissue Kit Print based in Delhi, India
https://www.srgroupchemical.com/dna-ffpe-tissue-kit-print-5473801.html
Comparison of whole-exome sequencing of matched fresh and formalin fixed paraffin embedded melanoma tumours: implications for...Comparison of whole-exome sequencing of matched fresh and formalin fixed paraffin embedded melanoma tumours: implications for...
The identification of recurrent driver mutations by whole-exome sequencing (WES) of fresh-frozen human cancers and the subsequent development of novel targeted therapies have recently transformed the treatment of many cancers including melanoma. In routine clinical practice, fresh-frozen tissue is rarely available and mutation testing usually needs to be carried out on archival formalin fixed, paraffin embedded (FFPE) tissue, from which DNA is typically fragmented, cross-linked and of lower quality. In this study we aimed to determine whether WES data generated from genomic DNA (gDNA) extracted from FFPE tissues can be produced reliably and of clinically-actionable standard. In this study of ten melanoma patients, we compared WES data produced from analysis of gDNA isolated from FFPE tumour tissue with that isolated from fresh-frozen tumour tissue from the same specimen. FFPE samples were sequenced using both Illuminas Nextera and NimbleGen SeqCap exome capture kits. To examine mutations between the
https://www.garvan.org.au/research/publications/13877
Characterization Of Genomic Alterations And Tcrβ Repertoire Of Tumor- Infiltrating Lymphocytes In Breast CancerCharacterization Of Genomic Alterations And Tcrβ Repertoire Of Tumor- Infiltrating Lymphocytes In Breast Cancer
Breast cancer still remains a major cause of morbidity and mortality among women in Qatar and worldwide. More recent studies indicate that the diversity and the composition of the entire set of antigen receptors within tumor-infiltrating lymphocytes (TILs) is strongly correlated with tumor prognosis and therapeutic response with breast cancer. Unfortunately, the relationship between somatic mutational load and TCR diversity of TILs across breast cancer still limited. For this purpose, first we characterized the somatic mutations of Formalin-Fixed Paraffin-Embedded breast cancer samples from 79 patients using NGS of a panel of cancer related genes. Second, we classified and identified the TCRß repertoire for these 11 samples using the ImmunoSEQ platform. Preliminary data demonstrated that the 11 patients had high diversity of TCRß-CDR3 within the tumors. However, there was no statistically significant association between the somatic mutational loads in the gene panels we sequenced and the ...
https://qspace.qu.edu.qa/handle/10576/11383
Immunohistochemical & Immunoblot Analysis of NIS (Na+/I-Symporter) in Archival & Frozen Tissue Sample - CareAcross
The goal of this study is to study NIS expression in benign and malignant breast and thyroid samples using archival formalin-fixed paraffin-embedded tissue sections.
https://www.careacross.com/clinical-trials/trial/NCT00568438
Jens Christian Jensenius - Research outputs - Research - Aarhus UniversityJens Christian Jensenius - Research outputs - Research - Aarhus University
Mannose-binding lectin (MBL) substitution: recovery of opsonic function in vivo lags behind MBL serum levels. / Brouwer, Nannette; Frakking, Florine N J; van de Wetering, Marianne D; van Houdt, Michel; Hart, Margreet; Budde, Ilona Kleine; Strengers, Paul F W; Laursen, Inga; Houen, Gunnar; Roos, Dirk; Jensenius, Jens C; Caron, Huib N; Dolman, Koert M; Kuijpers, Taco W.. In: Journal of Immunology, Vol. 183, No. 5, 2009, p. 3496-504.. Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review ...
https://pure.au.dk/portal/en/persons/jens-christian-jensenius
Comparing the proteome of snap frozen, RNAlater preserved, and formalin-fixed paraffin-embedded human tissue samples -...Comparing the proteome of snap frozen, RNAlater preserved, and formalin-fixed paraffin-embedded human tissue samples -...
TY - JOUR. T1 - Comparing the proteome of snap frozen, RNAlater preserved, and formalin-fixed paraffin-embedded human tissue samples. AU - Bennike, Tue Bjerg. AU - Kastaniegaard, Kenneth. AU - Padurariu, Simona AU - Gaihede, Michael. AU - Birkelund, Svend. AU - Andersen, Vibeke. AU - Stensballe, Allan. PY - 2016. Y1 - 2016. N2 - Large biobanks exist worldwide containing formalin-fixed, paraffin-embedded samples and samples stored in RNAlater. However, the impact of tissue preservation on the result of a quantative proteome analysis remains poorly described.Human colon mucosal biopsies were extracted from the sigmoideum and either immediately frozen, stabilized in RNAlater, or stabilized by formalin-fixation. In one set of biopsies, formalin stabilization was delayed for 30 min. The protein content of the samples was characterized by high throughput quantitative proteomics.We were able to identify a similar high number of proteins in the samples regardless of preservation method, with only minor ...
https://vbn.aau.dk/da/publications/comparing-the-proteome-of-snap-frozen-rnalater-preserved-and-form
Whole-exome sequencing and clinical interpretation of formalin-fixed, paraffin-embedded tumor samples to guide precision cancer...Whole-exome sequencing and clinical interpretation of formalin-fixed, paraffin-embedded tumor samples to guide precision cancer...
Translating whole-exome sequencing (WES) for prospective clinical use may have an impact on the care of patients with cancer; however, multiple innovations are necessary for clinical implementation. These include rapid and robust WES of DNA derived from formalin-fixed, paraffin-embedded tumor tissue, analytical output similar to data from frozen samples and clinical interpretation of WES data for prospective use. Here, we describe a prospective clinical WES platform for archival formalin-fixed, paraffin-embedded tumor samples. The platform employs computational methods for effective clinical analysis and interpretation of WES data. When applied retrospectively to 511 exomes, the interpretative framework revealed a long tail of somatic alterations in clinically important genes. Prospective application of this approach identified clinically relevant alterations in 15 out of 16 patients. In one patient, previously undetected findings guided clinical trial enrollment, leading to an objective ...
https://www.broadinstitute.org/publications/broad5750
Anti-Rat (Rattus) Insulin antibody for Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))Anti-Rat (Rattus) Insulin antibody for Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))
Compare & find the top performing anti-Rat (Rattus) Insulin antibody for Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)).
http://www.antibodies-online.com/nf-kappab-signaling-pathway-5/insulin-antibody-2308/rat-ihc-fp-10427/
Paraffin Tissue SectionsParaffin Tissue Sections
Formalin fixed, paraffin embedded (FFPE) whole tissue sections are ideal candidates for localizing DNA, RNA and protein markers. The tissues were fixed by formalin less than 48 hours, and then processed and sectioned. Two tissue sections with 5 µm (micro, micrometer) thickness are mounted on a SuperFrost Plus glass slide. US Biomax, Inc. paraffin tissue section is suitable for detection of genes and proteins expression in specific tissues of different species.. Features ...
https://www.biomax.us/Whole-Tissue-Sections/Paraffin-Tissue-Sections?page=2
Differential long-term stability of microRNAs and RNU6B snRNA in 12-20 year old archived formalin-fixed paraffin-embedded...Differential long-term stability of microRNAs and RNU6B snRNA in 12-20 year old archived formalin-fixed paraffin-embedded...
The first reported miRNA gene, lin-4, was described in C. elegans in 1993 [22]; the same year that the first surgical samples in this study were isolated. In a perfect world these samples would remain unchanged over time; however, it is well known that processing and storage can lead to significant RNA degradation [5, 6]. The influence of such parameters on miRNA detection and stability remains debatable.. A recent study of six miRNAs in colorectal tissue blocks stored for up to 28 years found no significant effects of sample block age on miRNA detection [12]. Similar observations were reported for miR-181b and 5S ribosomal RNA from blocks as old as ten years [8]. Deep sequencing analyses of miRNAs from multiple different types of tissue, stored for 2 to 9 years, have also found no significant change in miRNA detection with sample age [9]. In contrast, others have reported significant miRNA loss with extended FFPE block storage times. Comparison of miRNA expression from 1 to 11 year old FFPE ...
https://bmccancer.biomedcentral.com/articles/10.1186/s12885-016-3008-4
Expression profiling of formalin-fixed paraffin-embedded primary breast tumors using cancer-specific and whole genome gene...Expression profiling of formalin-fixed paraffin-embedded primary breast tumors using cancer-specific and whole genome gene...
The cDNA-mediated Annealing, extension, Selection and Ligation (DASL) assay has become a suitable gene expression profiling system for degraded RNA from paraffin-embedded tissue. We examined assay characteristics and the performance of the DASL 502-gene Cancer Panelv1 (1.5K) and 24,526-gene panel (24K) platforms at differentiating nine human epidermal growth factor receptor 2- positive (HER2+) and 11 HER2-negative (HER2-) paraffin-embedded breast tumors. Bland-Altman plots and Spearman correlations evaluated intra/inter-panel agreement of normalized expression values. Unequal-variance t-statistics tested for differences in expression levels between HER2 + and HER2 - tumors. Regulatory network analysis was performed using Metacore (GeneGo Inc., St. Joseph, MI). Technical replicate correlations ranged between 0.815-0.956 and 0.986-0.997 for the 1.5K and 24K panels, respectively. Inter-panel correlations of expression values for the common 498 genes across the two panels ranged between 0.485-0.573. Inter
https://bmcmedgenomics.biomedcentral.com/articles/10.1186/1755-8794-3-60/tables/1
Monkey Paraffin Tissue Sections : Zyagen, Life Science ProductsMonkey Paraffin Tissue Sections : Zyagen, Life Science Products
Zyagen : Monkey Paraffin Tissue Sections - Antibodies Kits, Reagents & Biochemical Tissue Sections RNA Products cDNA Products Genomic DNA Products Tissue Lysates Products Premade Northern Blots Premade Western Blots Recombinant Proteins & Peptides Tissue Microarrays
http://zyagen.com/index.php?main_page=index&cPath=3_62_375
Paraffin Tissue Section Panel - Human Disease Tissue, Alzheimers Disease, Multi-tissue I, 7 different tissues - BioChain...Paraffin Tissue Section Panel - Human Disease Tissue, Alzheimers Disease, Multi-tissue I, 7 different tissues - BioChain...
Paraffin Tissue Section Panel - Human Disease Tissue, Alzheimers Disease, Multi-tissue I, 7 different tissues - BioChain Institute Inc.
https://www.biochain.com/product/paraffin-tissue-section-panel-human-disease-tissue-alzheimers-disease-multi-tissue-i-7-different-tissues/
Diagnostic utility of thyroid transcription factor-1 expression in adenocarcinomas presenting in serous fluids<...
TY - JOUR. T1 - Diagnostic utility of thyroid transcription factor-1 expression in adenocarcinomas presenting in serous fluids. AU - Afify, Alaa M. AU - Al-Khafaji, Basim M.. PY - 2002. Y1 - 2002. N2 - OBJECTIVE: To evaluate the diagnostic utility of the immunohistochemical expression of thyroid transcription factorr-1 (TTF-1) in adenocarcinomas from serous fluid specimens. STUDY DESIGN: Archival paraffin-embedded cell blocks of serous fluids from 82 cases, including 34 cases of metastatic lung adenocarcinoma, 12 of metastatic ovarian adenocarcinoma, 12 of metastatic breast adenocarcinoma, 12 of metastatic gastrointestinal adenocarcinoma and 12 of malignant mesothelioma, were immunostained with anti-TTF-1. All the staining was carried out using a Ventana Automated System. Staining was evaluated according to the intensity of the nuclear staining (1+ to 4+) by two observers. RESULTS: Of the metastatic lung adenocarcinomas, 79% (27/34) expressed 3+ to 4+ reactivity against TTF-1. None of the ...
https://ucdavis.pure.elsevier.com/en/publications/diagnostic-utility-of-thyroid-transcription-factor-1-expression-i
Biomarker Validation | Department of Obstetrics, Gynecology and Womens Health - University of MinnesotaBiomarker Validation | Department of Obstetrics, Gynecology and Womens Health - University of Minnesota
Rizzardi AE, Johnson AT, Vogel RI, Pambuccian SE, Henriksen J, Skubitz AP, Metzger GJ, Schmechel SC.. Abstract: Immunohistochemical (IHC) assays performed on formalin-fixed paraffin-embedded (FFPE) tissue sections traditionally have been semi-quantified by pathologist visual scoring of staining. IHC is useful for validating biomarkers discovered through genomics methods as large clinical repositories of FFPE specimens support the construction of tissue microarrays (TMAs) for high throughput studies. Due to the ubiquitous availability of IHC techniques in clinical laboratories, validated IHC biomarkers may be translated readily into clinical use. However, the method of pathologist semi-quantification is costly, inherently subjective, and produces ordinal rather than continuous variable data. Computer-aided analysis of digitized whole slide images may overcome these limitations. Using TMAs representing 215 ovarian serous carcinoma specimens stained for S100A1, we assessed the degree to which data ...
https://www.obgyn.umn.edu/research/ovarian-cancer-early-detection-program/biomarker-validation
Detection of gene mutations in gastric cancer tissues using a commercial sequencing panelDetection of gene mutations in gastric cancer tissues using a commercial sequencing panel
Predicting malignancy is important for adequate adjuvant therapy in patients with cancer. Due to cancer being a genetic disease, the detection of gene mutations could be helpful in predicting the prognosis and efficacy of drugs. Gastric cancer is the fifth most common cancer and is the third leading cause of cancer associated mortality worldwide. Mutations in genes may correlate with clinical information in patients with gastric cancer after surgery and, therefore, may be useful for predicting the prognosis of this disease. In the present study, to assess the usefulness of a commercial sequencing panel, TruSeq® Amplicon‑Cancer Panel (Illumina), using a next‑generation sequencer (Illumina MiSeq), mutation analysis of fresh as well as formalin‑fixed paraffin‑embedded (FFPE) gastric cancer tissues was performed retrospectively. The study group comprised of 4 patients who underwent gastrectomy for gastric cancer. Cancer and normal stomach tissues were collected immediately following ...
https://www.spandidos-publications.com/10.3892/mco.2019.1926/abstract
A Study of Napabucasin Plus Nab-Paclitaxel With Gemcitabine in Adult Patients With Metastatic Pancreatic Adenocarcinoma - Full...A Study of Napabucasin Plus Nab-Paclitaxel With Gemcitabine in Adult Patients With Metastatic Pancreatic Adenocarcinoma - Full...
Only patients with available archival tumor tissue must consent to provision of, and Investigator(s) must confirm access to and agree to submit a representative formalin fixed paraffin block of tumor tissue in order that the specific correlative marker assays (Correlative Studies) of this protocol may be conducted. Submission of the tissue does not have to occur prior to randomization. Where local center regulations prohibit submission of blocks of tumor tissue, two 2 mm cores of tumor from the block and 5-20 unstained slides of whole sections of representative tumor tissue are preferred. Where it is not possible to obtain two 2 mm cores of tumor from the block, 5-20 unstained slides of representative tumor tissue are also acceptable. Where no previously resected or biopsied tumor tissue exists or is available, on the approval of the Sponsor/designated CRO, the patient may still be considered eligible for the study ...
https://clinicaltrials.gov/ct2/show/study/NCT02993731?term=CanStem111P&rank=1&show_locs=Y
MicroRNA analysisMicroRNA analysis
TATAA Biocenter is a Certified Service Provider for the 3D-Gene® instrument from Toray Industries.. TATAA Biocenter offers microarray analysis of miRNA on a novel microarray technology, the 3D-Gene™ from Toray Industries. It provides unprecedented sensitivity and reproducibility for microRNA analysis.. 3D-Gene™ DNA chips utilize patented resin slides to minimize background noise and microbead agitation technology to improve hybridization. Its unique 3D micro column structure define each spot to 100 µm in diameter for increased accuracy. It ensures a 100-fold increase in sensitivity and is ideal for challenging samples and low abundance targets. 3D-Gene™ is thus ideal for analyses of formalin fixed paraffin embedded (FFPE) tissue samples. This innovative technology also makes it possible to measure genes expressed at very low levels that might be undetected by other array platforms on the market.. 3D-Gene™ Human miRNA chips are constructed using the latest information available from ...
http://www.tataa.com/services/descriptions/microarray-analysis/
Tissue/Blood DNA Mini Kit (GS100, GS300) | GeneaidTissue/Blood DNA Mini Kit (GS100, GS300) | Geneaid
The gSYNC™ DNA Extraction Kit is a genomic DNA extraction kit optimized for genomic, mitochondrial and virus DNA purification from whole blood (fresh blood and frozen blood), serum, plasma, buffy coat, body fluids, cultured cells, tissue, rodent tails, ear punches, formalin-fixed paraffin-embedded tissue (FFPE), amniotic fl
http://www.geneaid.com/products/target-sample/tissue/tissue-blood-dna-mini-extraction-kit
Protocols and Video Articles Authored by Joshua EjdelmanProtocols and Video Articles Authored by Joshua Ejdelman
Joshua Ejdelman is the author of this article in the Journal of Visualized Experiments: Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
https://www.jove.com/author/Joshua_Ejdelman
Promising BRAF Mutation Test Takes 90 Minutes vs. Several We... : Oncology TimesPromising BRAF Mutation Test Takes 90 Minutes vs. Several We... : Oncology Times
For the study, the researchers tested the MDx platform in 79 archived tumor samples with BRAF mutation status established using the CLIA-approved method. The team used formalin-fixed paraffin-embedded tumor samples (1x to 5x of 10 μm shavings) from advanced cancer patients who had tested positive for V600 mutations using fPCR-based sequencing at CLIA-certified molecular diagnostic laboratory. Agreement between the MDx platform and the certified laboratorys results were then compared, and different methods and treatment outcomes with BRAF/MEK inhibitors were also analyzed.. The CLIA-approved method was in agreement for 75 of the samples. In all, 34 samples were from melanoma patients, 20 from colorectal cancer patients, six from papillary thyroid cancer, and 15 from patients with other cancers.. A total of 49 samples were V600 mutation-positive and 30 were wild-type BRAF mutations. In 70 patients the same tissue block was used for both MDx and CLIA samples, and agreement was found in 67. We ...
https://journals.lww.com/oncology-times/Fulltext/2013/12250/Promising_BRAF_Mutation_Test_Takes_90_Minutes_vs_.11.aspx
CD68, CD163, and matrix metalloproteinase 9 (MMP-9) co-localization in breast tumor microenvironment predicts survival...CD68, CD163, and matrix metalloproteinase 9 (MMP-9) co-localization in breast tumor microenvironment predicts survival...
The role of tumor-associated macrophages (TAMs) in the cancer immune landscape and their potential as treatment targets or modulators of response to treatment are gaining increasing interest. TAMs display high molecular and functional complexity. Therefore their objective assessment as breast cancer biomarkers is critical. The aims of this study were to objectively determine the in situ expression and significance of TAM biomarkers (CD68, CD163, and MMP-9) in breast cancer and to identify subclasses of patients who could benefit from TAM-targeting therapies. We measured CD68, CD163, and MMP-9 protein expression in formalin-fixed paraffin-embedded tissues of breast carcinomas represented in tissue microarray format using multiplexed quantitative immunofluorescence (QIF) in two independent Yale cohorts: cohort A-n = 398, estrogen receptor-positive (ER+) and ER− cases-and the triple-negative breast cancer (TNBC)-only cohort B (n = 160). Associations between macrophage markers, ER status, and survival
https://breast-cancer-research.biomedcentral.com/articles/10.1186/s13058-018-1076-x
Imaging of multiple mRNA targets using quantum dot based in situ hybridization and spectral deconvolution in clinical biopsies....Imaging of multiple mRNA targets using quantum dot based in situ hybridization and spectral deconvolution in clinical biopsies....
Gene expression mapping using microarray analysis has identified useful gene signatures for predicting outcome. However, little of this has been translated into clinically effective diagnostic tools as microarrays require high quality fresh-frozen tissue samples. We describe a methodology of multiplexed in situ hybridization (ISH) using a novel combination of quantum dot (QD)-labeled oligonucleotide probes and spectral imaging analysis in routinely processed, formalin-fixed paraffin embedded human biopsies. The conditions for QD-ISH were optimized using a poly d(T) oligonucleotide in decalcified bone marrow samples. Single and multiplex QD-ISH was performed in samples with acute leukemia and follicular lymphoma using oligonucleotide probes for myeloperoxidase, bcl-2, survivin, and XIAP. Spectral imaging was used for post hybridization tissue analysis, enabling separation of spatially colocalized signals. The method allows quantitative characterization of multiple gene expression using ...
https://www.broadinstitute.org/publications/broad3622
Genome-wide massively parallel sequencing of formaldehyde fixed-paraffin embedded (FFPE) tumor tissues for copy-number- and...Genome-wide massively parallel sequencing of formaldehyde fixed-paraffin embedded (FFPE) tumor tissues for copy-number- and...
The application of second generation sequencing techniques on small amounts of FFPE material opens up the possibility to analyze tissue samples which have been collected during routine clinical work as well as in the context of clinical trials. This is in particular important since FFPE samples are …
https://pubmed.ncbi.nlm.nih.gov/19440246/?dopt=Abstract
Whole Genome Amplification - WGA from Fixed Tissue Samples - QIAGENWhole Genome Amplification - WGA from Fixed Tissue Samples - QIAGEN
The archives of formalin-fixed, paraffin-embedded (FFPE) tissue sections represent a valuable and extensive source of material for biomedical research, however, DNA derived from FFPE samples is usuallyl highly degraded and cross-linked due to fixation. This section describes innovative REPLI-g technology that enables the successful amplification of fragmented and FFPE DNA samples.
https://www.qiagen.com/bg/service-and-support/learning-hub/technologies-and-research-topics/wga/fixed-samples/?Print=1
Gene expression variation between distinct areas of breast cancer measured from paraffin-embedded tissue cores | BMC Cancer |...Gene expression variation between distinct areas of breast cancer measured from paraffin-embedded tissue cores | BMC Cancer |...
Diagnosis and prognosis of breast cancer are still mainly based on clinical, histological and immunohistochemical parameters, which are at best semi-quantitative [1, 2]. Recently, molecular characterization of breast cancer has greatly increased the understanding of biological pathways that are altered during neoplastic transformation. Molecular markers have a great impact on elucidating the biological variance within tumors, they allow new and more accurate classifications and they have the potential to improve diagnosis, estimation of prognosis and treatment decisions in individual patients [3-6].. Most gene expression studies are based on fresh frozen material which, in most instances, is not readily available, as surgical samples are usually fixed in formalin. Unfortunately, RNA derived from formalin-fixed, paraffin-embedded (FFPE) material is considerably fragmented and chemically modified, often impairing gene expression measurement using standard procedures. We developed a simple and ...
https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-8-343
Paraffin-embedded Tissue Fragment Suspension (PETFS): A Novel Method for Quality Control Preparation in Immunohistochemistry -...
Paraffin-embedded Tissue Fragment Suspension (PETFS): A Novel Method for Quality Control Preparation in Immunohistochemistry Academic Article ...
https://experts.mcmaster.ca/display/publication968857
The effect of fixation type on DNA extracted from paraffin-embedded tissue for PCR studies in dermatopathology - Fingerprint ...
Fingerprint Dive into the research topics of The effect of fixation type on DNA extracted from paraffin-embedded tissue for PCR studies in dermatopathology. Together they form a unique fingerprint. ...
https://moh-it.pure.elsevier.com/en/publications/the-effect-of-fixation-type-on-dna-extracted-from-paraffin-embedd/fingerprints/
IHC (Paraffin section) Antibodies - Support (IHC-P): Novus BiologicalsIHC (Paraffin section) Antibodies - Support (IHC-P): Novus Biologicals
Novus offers a wide range of antibodies for immunohistochemistry on paraffin sections. View our catalog of IHC-P products plus protocols and tips.
https://www.novusbio.com/applications/Immunohistochemistry-Paraffin?related_diseases=Scoliosis,%20Unspecified
IHC (Paraffin section) Antibodies - Support (IHC-P): Novus BiologicalsIHC (Paraffin section) Antibodies - Support (IHC-P): Novus Biologicals
Novus offers a wide range of antibodies for immunohistochemistry on paraffin sections. View our catalog of IHC-P products plus protocols and tips.
https://www.novusbio.com/applications/Immunohistochemistry-Paraffin?common_name=PTP%20epsilon
Carbonic anhydrase III, muscle specificCarbonic anhydrase III, muscle specific
Typing of skeletal muscle fibers in paraffin embedded sections". Histochemistry. 83 (3): 231-5. doi:10.1007/bf00953989. PMID ...
https://en.wikipedia.org/wiki/Carbonic_anhydrase_III,_muscle_specific
MicrotechniqueMicrotechnique
Paraffin wax, whose melting point is from 56 to 62℃, is commonly used for embedding. Since few plant tissues have a color, ... This process is called embedding. The substance used to embed tissue is embedding media, which is chosen depends on the ... A tissue specimen can keep for several years after finishing embedding this tissue into the wax. Paraffin wax, which is soft ... "Sectioning of paraffin-embedded tissue protocol , Abcam". www.abcam.com. Retrieved 2019-05-19. "Advanced Sectioning Techniques ...
https://en.wikipedia.org/wiki/Microtechnique
Breast cancer classificationBreast cancer classification
The test is run on formalin fixed, paraffin-embedded tissue. Oncotype results are reported as a Recurrence Score (RS), where a ... The test is run on formalin fixed, paraffin-embedded tissue. MammaPrint traditionally used rapidly frozen tissue but a room ... October 2005). "Gene expression profiles in paraffin-embedded core biopsy tissue predict response to chemotherapy in women with ...
https://en.wikipedia.org/wiki/Breast_cancer_classification
2,2-Dimethoxypropane2,2-Dimethoxypropane
Poul Prentø (1978). "Rapid dehydration--clearing with 2,2-dimethoxypropane for paraffin embedding". J. Histochem. Cytochem. 26 ...
https://en.wikipedia.org/wiki/2,2-Dimethoxypropane
Laser capture microdissectionLaser capture microdissection
Frozen and paraffin embedded archival tissue may also be used. Emmert-Buck MR, Bonner RF, Smith PD, Chuaqui RF, Zhuang Z, ...
https://en.wikipedia.org/wiki/Laser_capture_microdissection
Reduced representation bisulfite sequencingReduced representation bisulfite sequencing
Formalin-fixed and paraffin-embedded inputs can also be used. In the specific protocol steps, there are also some limitations. ...
https://en.wikipedia.org/wiki/Reduced_representation_bisulfite_sequencing
CD163CD163
Lau SK, Chu PG, Weiss LM (November 2004). "CD163: a specific marker of macrophages in paraffin-embedded tissue samples". ...
https://en.wikipedia.org/wiki/CD163
Bread loafingBread loafing
The cut sections are mounted by embedding in paraffin or frozen medium. The cut edge is then thinly sliced with a microtome or ...
https://en.wikipedia.org/wiki/Bread_loafing
Trajectory inferenceTrajectory inference
... paraffin-embedded cancer tissue". Proceedings of the National Academy of Sciences. 110 (29): 11982-11987. Bibcode:2013PNAS.. ...
https://en.wikipedia.org/wiki/Trajectory_inference
Imaging cycler microscopyImaging cycler microscopy
... paraffin-embedded cancer tissue". Proceedings of the National Academy of Sciences. 110 (29): 11982-11987. Bibcode:2013PNAS.. ...
https://en.wikipedia.org/wiki/Imaging_cycler_microscopy
HistologyHistology
For light microscopy, paraffin wax is the most frequently used embedding material. Paraffin is immiscible with water, the main ... However, extraction and analysis of nucleic acids and proteins from formalin-fixed, paraffin-embedded tissues is possible using ... and formalin-fixed paraffin-embedded human tissue samples". EuPA Open Proteomics. 10: 9-18. doi:10.1016/j.euprot.2015.10.001. ... reported that he had for some years embedded his specimens in paraffin. The 1906 Nobel Prize in Physiology or Medicine was ...
https://en.wikipedia.org/wiki/Histology
AcetoneAcetone
Engbaek, K; Johansen, KS; Jensen, ME (February 1979). "A new technique for Gram staining paraffin-embedded tissue". Journal of ...
https://en.wikipedia.org/wiki/Acetone
PaucimannosylationPaucimannosylation
"N-glycan MALDI Imaging Mass Spectrometry on Formalin-Fixed Paraffin-Embedded Tissue Enables the Delineation of Ovarian Cancer ... "Unlocking Cancer Glycomes from Histopathological Formalin-fixed and Paraffin-embedded (FFPE) Tissue Microdissections". ...
https://en.wikipedia.org/wiki/Paucimannosylation
Potassium permanganatePotassium permanganate
July 2001). "Chemical typing of amyloid protein contained in formalin-fixed paraffin-embedded biopsy specimens". American ...
https://en.wikipedia.org/wiki/Potassium_permanganate
ImmunostainingImmunostaining
Many antigens can be successfully demonstrated in formalin-fixed paraffin-embedded tissue sections. However, some antigens will ... difficulty in cutting over paraffin sections, and the need for frozen storage. Alternatively, vibratome sections do not require ...
https://en.wikipedia.org/wiki/Immunostaining
Virtual karyotypeVirtual karyotype
Because SNP array karyotyping can be performed on paraffin embedded tumors, it is an attractive option when tumor cells fail to ... Array-based karyotyping performs well on paraffin embedded tumors and is amenable to routine clinical use. In addition, recent ... CLIA-certified laboratories offering testing on tumors include Creighton Medical Laboratories (fresh and paraffin embedded ... "Optimization of the Affymetrix GeneChip Mapping 10K 2.0 Assay for Routine Clinical Use on Formalin Fixed Paraffin Embedded ...
https://en.wikipedia.org/wiki/Virtual_karyotype
L-form bacteriaL-form bacteria
paratuberculosis in paraffin embedded tissues from animals with Johne's disease by in situ hybridization". J. Microbiol. ...
https://en.wikipedia.org/wiki/L-form_bacteria
Chromogenic in situ hybridizationChromogenic in situ hybridization
Summersgill, B. M.; Shipley, J. M. (2010). "Fluorescence in Situ Hybridization Analysis of Formalin Fixed Paraffin Embedded ... "Chromogenic in situ hybridization and p16/Ki67 dual staining on formalin-fixed paraffin-embedded cervical specimens: ... Tissue samples are securely attached to a surface, which is usually a glass slide, with paraffin. The tissue samples must then ... be washed and heated several times to remove any paraffin before the hybridization step. After this, the sample has to undergo ...
https://en.wikipedia.org/wiki/Chromogenic_in_situ_hybridization
Mitotic indexMitotic index
1998). "Correlation between mitotic and Ki-67 labeling indices in paraffin-embedded carcinoma specimens". Human Pathology. 29 ( ...
https://en.wikipedia.org/wiki/Mitotic_index
TrastuzumabTrastuzumab
HER2 amplification can be detected by virtual karyotyping of formalin-fixed paraffin embedded tumor. Virtual karyotyping has ... The HER receptors are proteins that are embedded in the cell membrane and communicate molecular signals from outside the cell ( ...
https://en.wikipedia.org/wiki/Trastuzumab
RhabdomyosarcomaRhabdomyosarcoma
... an immunohistochemical study in paraffin-embedded tissues". Modern Pathology. 13 (9): 988-993. doi:10.1038/modpathol.3880179. ...
https://en.wikipedia.org/wiki/Rhabdomyosarcoma
Complement receptor 2Complement receptor 2
In more conventional paraffin-embedded tissue samples, only the FDCs retain the staining pattern. As a result, CR2, more ...
https://en.wikipedia.org/wiki/Complement_receptor_2
Douglas Houghton CampbellDouglas Houghton Campbell
He developed a technique to embed plant material in paraffin to make fine cross-sections; he was one of the first if not the ...
https://en.wikipedia.org/wiki/Douglas_Houghton_Campbell
Renal cell carcinomaRenal cell carcinoma
Array-based karyotyping performs well on paraffin embedded tumours and is amenable to routine clinical use. See also Virtual ... "Optimization of the Affymetrix GeneChip Mapping 10K 2.0 Assay for routine clinical use on formalin-fixed paraffin-embedded ...
https://en.wikipedia.org/wiki/Renal_cell_carcinoma
ImmunohistochemistryImmunohistochemistry
For formalin-fixed paraffin-embedded tissues, antigen-retrieval is often necessary, and involves pre-treating the sections with ... Before sectioning, the tissue sample may be embedded in a medium, like paraffin wax or cryomedia. Sections can be sliced on a ... staining and troubleshooting Immunofluorescent Staining of Paraffin-Embedded Tissue (IF-P) IHC Tip 1: Antigen retrieval - ...
https://en.wikipedia.org/wiki/Immunohistochemistry
Tumor BankTumor Bank
... and formalin-fixed paraffin-embedded human colon mucosal biopsies". Data in Brief. 6: 942-947. doi:10.1016/j.dib.2016.01.061. ... and formalin-fixed paraffin-embedded human tissue samples". EuPA Open Proteomics. 10: 9-18. doi:10.1016/j.euprot.2015.10.001. ... "RNA extraction from ten year old formalin-fixed paraffin-embedded breast cancer samples: a comparison of column purification ...
https://en.wikipedia.org/wiki/Tumor_Bank
Wind turbine designWind turbine design
Embedding paraffin wax-coated copper wires in a fiber reinforced polymer creates a network of tubes. Using a catalyst, these ... It is most common to observe lightning strikes on the tips of the blades, especially in rainy weather due to embedded copper ...
https://en.wikipedia.org/wiki/Wind_turbine_design
Uncombable hair syndromeUncombable hair syndrome
An alternative method for confirming a UHS diagnosis molecularly is by embedding UHS hairs into paraffin. Due to the atypical ...
https://en.wikipedia.org/wiki/Uncombable_hair_syndrome
Fluorescence in situ hybridizationFluorescence in situ hybridization
First, cells, circulating tumor cells (CTCs), formalin-fixed paraffin-embedded (FFPE), or frozen tissue sections are fixed. ...
https://en.wikipedia.org/wiki/Fluorescence_in_situ_hybridization
Collagen hybridizing peptideCollagen hybridizing peptide
CHPs can stain frozen tissue sections, formalin-fixed paraffin embedded (FFPE) sections, as well as fresh tissues. CHP is ...
https://en.wikipedia.org/wiki/Collagen_hybridizing_peptide
India inkIndia ink
... during which tissue samples are bathed in alcohol and xylene and then embedded in paraffin wax. When viewed under the ...
https://en.wikipedia.org/wiki/India_ink
Sverdlovsk anthrax leakSverdlovsk anthrax leak
The samples had been fixed in formalin and embedded in paraffin and the DNA was, as a result, badly degraded. Nevertheless, the ...
https://en.wikipedia.org/wiki/Sverdlovsk_anthrax_leak
Timeline of United States inventions (1890-1945)Timeline of United States inventions (1890-1945)
They usually consist of an embedded hardware unit connected to a pair of headphones and a feedback button, sometimes controlled ... and other additives like paraffin or petroleum solvents to aid in ignition. The design of the charcoal briquette was invented ...
https://en.wikipedia.org/wiki/Timeline_of_United_States_inventions_(1890–1945)
SSX1SSX1
RT-PCR detection of SYT-SSX1/2 fusion transcripts in paraffin-embedded tissue". Med. Sci. Monit. 11 (3): MT1-7. PMID 15735574. ...
https://en.wikipedia.org/wiki/SSX1
Ki-67 (protein)Ki-67 (protein)
... is that it can be used on formalin-fixed paraffin-embedded sections, after heat-mediated antigen retrieval (see next section ...
https://en.wikipedia.org/wiki/Ki-67_(protein)
Tissue cytometryTissue cytometry
In this process, a tissue sample, either formalin-fixed paraffin-embedded (FFPE) or frozen tissue section, also referred to as ... fixed in formalin and embedded on paraffin. These tissue sections are serially cut in a microtome to provide thin sections, ...
https://en.wikipedia.org/wiki/Tissue_cytometry
Automated tissue image analysisAutomated tissue image analysis
... embedded in paraffin, thin sectioned typically to 4 um slices, then mounted onto at least two barcoded slides (a control and a ... test). Next the paraffin is removed from the tissue, the tissue is rehydrated, then stained. Any inconsistency in these ...
https://en.wikipedia.org/wiki/Automated_tissue_image_analysis
Cholesterol embolismCholesterol embolism
... embedded in paraffin wax). The cholesterol crystals may be associated with macrophages, including giant cells, and eosinophils ...
https://en.wikipedia.org/wiki/Cholesterol_embolism
BatmobileBatmobile
The Arkham Knight Batmobile-Urban Assault Vehicle-has a "Reconfigurable Embedded System", featuring two modes between which it ... gasoline paraffin mixture) Torque: 1,750 pound force-feet (2,370 N⋅m) at 98.7% ROS Launchers: Side-mounted grappling hook ...
https://en.wikipedia.org/wiki/Batmobile
Tutankhamuns mummyTutankhamun's mummy
Later examinations have shown that the bone fragments are loose inside the skull and not embedded within the embalming resin as ... the fabric was stabilised with a very thin layer of liquid paraffin wax before a central incision was made from the edge of the ... each body part was treated with hot paraffin wax to stabilise it. The hands and feet were later reattached with resin. Lastly, ...
https://en.wikipedia.org/wiki/Tutankhamun's_mummy
Chromatin immunoprecipitationChromatin immunoprecipitation
Pathology-ChIP (PAT-ChIP): This technique allows ChIP from pathology formalin-fixed and paraffin-embedded tissues and thus the ...
https://en.wikipedia.org/wiki/Chromatin_immunoprecipitation
Rock analogs for structural geologyRock analogs for structural geology
Rossetti, F., Ranalli, G., and Faccenna, C., 1999, Rheological properties of paraffin as an analogue material for viscous ... Cobbold, P.R. (August 1975). "Fold propagation in single embedded layers". Tectonophysics. 27 (4): 333-351. Bibcode:1975Tectp.. ... Duarte, João C.; Schellart, Wouter P.; Cruden, Alexander R. (June 2014). "Rheology of petrolatum-paraffin oil mixtures: ...
https://en.wikipedia.org/wiki/Rock_analogs_for_structural_geology
MicrotomeMicrotome
Typical applications for this design of microtome are of the preparation of large samples, such as those embedded in paraffin ... and embedded in melted paraffin, which when cooled forms a solid block. The tissue is then cut in the microtome at thicknesses ... in a process known as embedding. This is achieved by the inflow of a liquid substance around the sample, such as paraffin (wax ... For hard materials, such as a sample embedded in a synthetic resin, this design of microtome can allow good "semi-thin" ...
https://en.wikipedia.org/wiki/Microtome
PAS diastase stainPAS diastase stain
PAS-D is a stain often used by pathologists as an ancillary study in making a histologic diagnosis on paraffin-embedded tissue ...
https://en.wikipedia.org/wiki/PAS_diastase_stain
Diesel generatorDiesel generator
Other high viscosity fuels like tallow, vegetable oil or paraffin wax can be used with standard fuel injectors if the fuel is ... "Overcoming Barriers To Scheduling Embedded Generation To Support Distribution Networks" (PDF). BERR. Archived from the original ... "Overcoming Barriers To Scheduling Embedded Generation To Support Distribution Networks" Increasing use of banks of diesel ...
https://en.wikipedia.org/wiki/Diesel_generator
MALDI imagingMALDI imaging
"MALDI Imaging Mass Spectrometry Profiling of N-Glycans in Formalin-Fixed Paraffin Embedded Clinical Tissue Blocks and Tissue ... When ready to be analyzed, the tissue is embedded in a gelatin media which supports the tissue while it is being cut, while ...
https://en.wikipedia.org/wiki/MALDI_imaging
Cognition Network TechnologyCognition Network Technology
The intended use of Definiens Tissue Studio is for biomarker translational research in formalin-fixed, paraffin-embedded tissue ...
https://en.wikipedia.org/wiki/Cognition_Network_Technology
CUBICCUBIC
... in this case used for diagnostic of human pathologies both on native and formalin fixed paraffin embedded tissues. CLARITY ...
https://en.wikipedia.org/wiki/CUBIC
StainingStaining
Pieces of tissue may be embedded in paraffin wax to increase their mechanical strength and stability and to make them easier to ...
https://en.wikipedia.org/wiki/Staining
ETV6ETV6
... of the ETV6-NTRK3 chimeric RNA of infantile fibrosarcoma/cellular congenital mesoblastic nephroma in paraffin-embedded tissue: ...
https://en.wikipedia.org/wiki/ETV6
Frozen section procedureFrozen section procedure
The quality of the slides produced by frozen section is of lower quality than formalin fixed paraffin embedded tissue ... Covering the specimen with embedding medium Applying a conductor (unless it's a thin specimen that needs to stand on its side) ... The ultrastructural properties can be studied without embedding of the tissue, and so the molecular conservation is better. ... Using freeze spray to quicken the freezing if available Breaking off any embedding medium that reaches below the chuck's plate ...
https://en.wikipedia.org/wiki/Frozen_section_procedure
Immunohistochemistry : Paraffin-embedded IHC | Cell Signaling TechnologyImmunohistochemistry : Paraffin-embedded IHC | Cell Signaling Technology
Visit CellSignal.com to view our Immunohistochemistry materials including Paraffin-embedded IHC & more. CST - Customer ... IF-paraffin (4) IHC-Leica BOND (356) IHC-paraffin (1566) Immunofluorescence (Immunocytochemistry) (1739) Immunohistochemistry ( ...
https://www.cellsignal.com/browse/immunohistochemistry-frozen/primary-antibodies?N=4294960086+102236+4294956287
Expanding the Utilization of Formalin-Fixed, Paraffin-Embedded Archives: Feasibility of miR-Seq for Disease Exploration and...Expanding the Utilization of Formalin-Fixed, Paraffin-Embedded Archives: Feasibility of miR-Seq for Disease Exploration and...
MicroRNAs (miRNAs) have been increasingly investigated for their predictive value, and formalin-fixed paraffin-embedded biopsy ... In conclusion, formalin-fixed paraffin-embedded biopsy tissues are a viable source of miRNA-sequencing material. Our results ... Keywords: clear cell renal cell carcinoma/ccRCC; formalin-fixed paraffin-embedded/FFPE; miR-155; microRNA/miRNA; next ... Expanding the Utilization of Formalin-Fixed, Paraffin-Embedded Archives: Feasibility of miR-Seq for Disease Exploration and ...
https://pubmed.ncbi.nlm.nih.gov/29534467/
Gene Expression Profiles in Paraffin-Embedded Core Biopsy Tissue Predict Response to Chemotherapy in Women with Locally...Gene Expression Profiles in Paraffin-Embedded Core Biopsy Tissue Predict Response to Chemotherapy in Women with Locally...
89 of the Milan patients paraffin-embedded samples were analyzable. After paraffin removal, RNA was extracted and quantitative ... Gene Expression Profiles in Paraffin-Embedded Core Biopsy Tissue Predict Response to Chemotherapy in Women with Locally ... Gene Expression Profiles in Paraffin-Embedded Core Biopsy Tissue Predict Response to Chemotherapy in Women with Locally ... but recent studies have demonstrated that a sufficient amount of RNA can be extracted from paraffin-embedded samples. ...
https://www.oncolink.org/oncolink-library/journal-scans/breast-cancer/gene-expression-profiles-in-paraffin-embedded-core-biopsy-tissue-predict-response-to-chemotherapy-in-women-with-locally-advanced-cancer
Development of a robust protocol for gene expression analysis using formalin-fixed, paraffin-embedded liver transplant biopsy...Development of a robust protocol for gene expression analysis using formalin-fixed, paraffin-embedded liver transplant biopsy...
Development of a robust protocol for gene expression analysis using formalin-fixed, paraffin-embedded liver transplant biopsy ... Development of a robust protocol for gene expression analysis using formalin-fixed, paraffin-embedded liver transplant biopsy ... Development of a robust protocol for gene expression analysis using formalin-fixed, paraffin-embedded liver transplant biopsy ...
https://jcp.bmj.com/content/66/9/815.share
DNA Flow Cytometry and Histopathological Grading of Paraffin-embedded Prostate Biopsy Specimens in a Survival Study1 | Cancer...DNA Flow Cytometry and Histopathological Grading of Paraffin-embedded Prostate Biopsy Specimens in a Survival Study1 | Cancer...
DNA Flow Cytometry and Histopathological Grading of Paraffin-embedded Prostate Biopsy Specimens in a Survival Study1 Sven ... Methods to disintegrate old paraffin-embedded tissue blocks for the application of DNA flow cytometry open up new possibilities ... Sven Lundberg, John Carstensen, Ingemar Rundquist; DNA Flow Cytometry and Histopathological Grading of Paraffin-embedded ... Plugs of histologically identified tissue from benign and tumor areas were sampled from paraffin blocks of prostate biopsy ...
https://aacrjournals.org/cancerres/article/47/7/1973/492288/DNA-Flow-Cytometry-and-Histopathological-Grading
Mutation screening using formalin-fixed paraffin-embedded tissues: a stratified approach according to DNA quality.
... ... DNA samples from formalin-fixed paraffin-embedded tissues are highly degraded with variable quality, and this imposes a big ... Humans, Formaldehyde, DNA, Paraffin Embedding, Tissue Fixation, Reproducibility of Results, Polymerase Chain Reaction, DNA ... 2018). Mutation screening using formalin-fixed paraffin-embedded tissues: a stratified approach according to DNA quality.. Lab ...
https://www.repository.cam.ac.uk/handle/1810/277615
Evaluating the Feasibility of DNA Methylation Analyses Using Long-Term Archived Brain Formalin-Fixed Paraffin-Embedded Samples Evaluating the Feasibility of DNA Methylation Analyses Using Long-Term Archived Brain Formalin-Fixed Paraffin-Embedded Samples
p,We here characterize the usability of archival formalin-fixed paraffin-embedded (FFPE) brain tissue as a resource for genetic ... We here characterize the usability of archival formalin-fixed paraffin-embedded (FFPE) brain tissue as a resource for genetic ... Evaluating the Feasibility of DNA Methylation Analyses Using Long-Term Archived Brain Formalin-Fixed Paraffin-Embedded Samples ...
https://www.diagenode.com/en/publications/view/3108
Specimen Submission Guidelines for Pathologic Evaluation of Unexplained Illness due to Possibly Infectious Etiology |...Specimen Submission Guidelines for Pathologic Evaluation of Unexplained Illness due to Possibly Infectious Etiology |...
Paraffin-embedded tissue blocks. In general, this is the preferred specimen and is especially important to submit in cases ... After fixation, if not paraffin-embedded, tissues SHOULD be transferred to 70% ethanol for long term storage and for shipping. ... neutral buffered formalin be submitted in addition to paraffin blocks. The volume of formalin used to fix tissues should be 10x ...
https://www.cdc.gov/ncezid/dhcpp/idpb/specimen-submission/general-unexplained.html
SMRT Sequencing of DNA and RNA samples extracted from formalin-fixed and paraffin-embedded tissues - PacBioSMRT Sequencing of DNA and RNA samples extracted from formalin-fixed and paraffin-embedded tissues - PacBio
The data presented here demonstrates single molecule sequencing of DNA samples extracted from tissues embedded in FFPE. ... Recent advances in next-generation sequencing have led to the increased use of formalin-fixed and paraffin-embedded (FFPE) ... SMRT Sequencing of DNA and RNA samples extracted from formalin-fixed and paraffin-embedded tissues Author(s): Baybayan, Primo ... Recent advances in next-generation sequencing have led to the increased use of formalin-fixed and paraffin-embedded (FFPE) ...
https://www.pacb.com/proceedings/smrt-sequencing-of-dna-and-rna-samples-extracted-from-formalin-fixed-and-paraffin-embedded-tissues/
Flow cytometric analysis of cellular DNA content in paraffin wax-embedded specimens of canine mammary tumoursFlow cytometric analysis of cellular DNA content in paraffin wax-embedded specimens of canine mammary tumours
... E. Scanziani. ... Flow cytometric analysis of DNA content was performed on 72 formalin-fixed, paraffin wax-embedded samples of canine mammary ... Flow cytometric analysis of cellular DNA content in paraffin wax-embedded specimens of canine mammary tumours / E. Scanziani, M ... Flow cytometric analysis of DNA content was performed on 72 formalin-fixed, paraffin wax-embedded samples of canine mammary ...
https://air.unimi.it/handle/2434/187456
Progression-specific genes identified in microdissected formalin-fixed and paraffin-embedded tissue containing matched ductal...Progression-specific genes identified in microdissected formalin-fixed and paraffin-embedded tissue containing matched ductal...
Here, we describe a transcriptomic profiling analysis of matched formalin-fixed and paraffin-embedded (FFPE) DCIS and IBC ... Formalin-fixed and paraffin-embedded tumour tissue. For expression analysis paraffin blocks from 15 surgically excised primary ... In the current study we used formalin-fixed, paraffin-embedded (FFPE) tissue samples from 15 patients with both DCIS and IBC ( ... Here, we describe a transcriptomic profiling analysis of matched formalin-fixed and paraffin-embedded (FFPE) DCIS and IBC ...
https://bmcmedgenomics.biomedcentral.com/articles/10.1186/s12920-018-0403-5
Molecular inversion probe array for the genetic evaluation of stillbirth using formalin-fixed, paraffin-embedded tissue<...Molecular inversion probe array for the genetic evaluation of stillbirth using formalin-fixed, paraffin-embedded tissue<...
Molecular inversion probe array for the genetic evaluation of stillbirth using formalin-fixed, paraffin-embedded tissue. In: ... Molecular inversion probe array for the genetic evaluation of stillbirth using formalin-fixed, paraffin-embedded tissue. / Rowe ... Molecular inversion probe array for the genetic evaluation of stillbirth using formalin-fixed, paraffin-embedded tissue. ... Molecular inversion probe array for the genetic evaluation of stillbirth using formalin-fixed, paraffin-embedded tissue. ...
https://researchexperts.utmb.edu/en/publications/molecular-inversion-probe-array-for-the-genetic-evaluation-of-sti
Immunohistochemical detection of cell-cycle associated markers on paraffin embedded and formalin fixed needle biopsies of...Immunohistochemical detection of cell-cycle associated markers on paraffin embedded and formalin fixed needle biopsies of...
In conclusion, paraffin embedded and formalin fixed PC needle biopsies exhibit a higher diagnostic PCNA/Cyclin than Ki-67/MIB1 ... In conclusion, paraffin embedded and formalin fixed PC needle biopsies exhibit a higher diagnostic PCNA/Cyclin than Ki-67/MIB1 ... Paraffin embedded and formalin fixed needle biopsies of prostate cancer (PC) were used to immunocytochemically detect the p120 ... Paraffin embedded and formalin fixed needle biopsies of prostate cancer (PC) were used to immunocytochemically detect the p120 ...
https://iris.unimore.it/handle/11380/304751
Clinical whole-genome sequencing from routine formalin-fixed, paraffin-embedded specimens: pilot study for the 100,000 Genomes...
Clinical whole-genome sequencing from routine formalin-fixed, paraffin-embedded specimens: pilot study for the 100,000 Genomes ... Clinical whole-genome sequencing from routine formalin-fixed, paraffin-embedded specimens: pilot study for the 100,000 Genomes ...
https://www.well.ox.ac.uk/publications/822892?91be301a-72c7-11ed-8572-061b866677b4
Evaluation of DNA dilution extracted of paraffin-embedded material for PCR amplificationEvaluation of DNA dilution extracted of paraffin-embedded material for PCR amplification
KAWATA, LEANDRO TOYOJI et al. Evaluation of DNA dilution extracted of paraffin-embedded material for PCR amplification. RPG, ... Material and Method: Paraffin-embedded blocks from 30 patients with oropharynx squamous cell carcinomas, diagnosed and treated ... Objective: To evaluate the dilution influence of DNA purified from paraffin-embedded materials on β-globin PCR amplification. ... Dilution of the DNA extracted of paraffin-embedded materials did not modify statistically the amount of positive samples β- ...
http://revodonto.bvsalud.org/scielo.php?script=sci_abstract&pid=S0104-56952010000500005&lng=pt&nrm=iso&tlng=en
Novel skin phenotypes revealed by a genome-wide mouse reverse genetic screen | Nature CommunicationsNovel skin phenotypes revealed by a genome-wide mouse reverse genetic screen | Nature Communications
As part of the screen, we collected tail skin for paraffin embedding. This has created a valuable archive from which we can ... In addition, pieces of intact tail skin were paraffin embedded for detailed histology and DNA was extracted for verification of ... Tissue was fixed overnight in 10% neutral buffered formalin and was paraffin embedded. Sections were labelled with haematoxylin ... one for paraffin conventional histology and one for genomic DNA extraction. (b) WT epidermal wholemount image labelled for K14 ...
https://www.nature.com/articles/ncomms4540?error=cookies_not_supported
TISSUE EMBEDDING PARAFFIN WAX TISSUE-WAX PK.1KG - thelabwarehouse.comTISSUE EMBEDDING PARAFFIN WAX TISSUE-WAX PK.1KG - thelabwarehouse.com
... white paraffin wax blended with synthetic polymers and suitable for routine embedding and sectioning applications. Offers very ... white paraffin wax blended with synthetic polymers and suitable for routine embedding and sectioning applications. Offers very ... TISSUE EMBEDDING PARAFFIN W.... TISSUE EMBEDDING PARAFFIN WAX TISSUE-WAX PK.1KG. ...
https://www.thelabwarehouse.com/products/tissue-embedding-paraffin-wax-tissue-wax-pk-1kg
formalin-fixed paraffin-embedded Archives - The DNA Universe BLOGformalin-fixed paraffin-embedded Archives - The DNA Universe BLOG
formalin-fixed paraffin-embedded Archives - The DNA Universe ...
https://the-dna-universe.com/tag/formalin-fixed-paraffin-embedded/
Kuperwasser Lab | Immunohistochemistry on paraffin embedded sections (PJ Keller)Kuperwasser Lab | Immunohistochemistry on paraffin embedded sections (PJ Keller)
Shandon Cytospin 4 Standard Workflow Diagram Immunofluorescence for CK14 and CK18 on paraffin embedded sections ... De-paraffin and rehydrate slides *Incubate 5 each in xylenes (x2), then 3 each in 100% EtOH (x2), 95% EtOH (x2) and 70% EtOH ...
https://kuperwasser-lab.org/immunohistochemistry-on-paraffin-embedded-sections-pj-keller/
Tissue-Bank - FFPE Tissue Blocks Biochain Formalin-Fixed Paraffin-EmbeddedTissue-Bank - FFPE Tissue Blocks Biochain Formalin-Fixed Paraffin-Embedded
Paraffin Tissue SectionsParaffin Tissue Sections. April 21, 2021April 21, 2021 LievenLieven 0 Comments 3:16 pm ... Histology Services Paraffin tissue processing, embedding, sectioning, staining and frozen tissue sectioning Image Scanning ... We have pre-cut histology paraffin (FFPE) tissue sections, including normal human paraffin sections HuFPT, human cancer ...
https://www.tissue-bank.com/
Can you do immunofluorescence on paraffin embedded sections? - Durrell2012.comCan you do immunofluorescence on paraffin embedded sections? - Durrell2012.com
Incubate the tissue in a 65 °C paraffin bath for 2 times, 30 min each. Pour melted paraffin into paraffin block mold. Place the ... How thick are paraffin embedded sections that has been cut?. Trimming is normally done at a thickness of 10-30 µm. Cut sections ... Section the paraffin-embedded tissue block at 5-8 μm thickness on a microtome and float in a 40°C water bath containing ... How do you stain paraffin embedded tissue?. Immerse the tissue in 70% ethanol three times for 30 minutes each at room ...
https://durrell2012.com/can-you-do-immunofluorescence-on-paraffin-embedded-sections/
Immunohistochemical detection of thrombospondin-1 in formalin-fixed, paraffin-embedded tissue<...Immunohistochemical detection of thrombospondin-1 in formalin-fixed, paraffin-embedded tissue<...
Immunohistochemical detection of thrombospondin-1 in formalin-fixed, paraffin-embedded tissue. Gary D. Grossfeld, Shan Rong Shi ... Immunohistochemical detection of thrombospondin-1 in formalin-fixed, paraffin-embedded tissue. / Grossfeld, Gary D.; Shi, Shan ... Our results demonstrate that TSP can be reliably detected in paraffin-embedded tissue by immunohistochemical techniques that ... Immunohistochemical detection of thrombospondin-1 in formalin-fixed, paraffin-embedded tissue. In: Journal of Histochemistry ...
https://profiles.wustl.edu/en/publications/immunohistochemical-detection-of-thrombospondin-1-in-formalin-fix
Comprehensive assay for the molecular profiling of cancer by target enrichment from formalin-fixed paraffin-embedded specimens<...
Comprehensive assay for the molecular profiling of cancer by target enrichment from formalin-fixed paraffin-embedded specimens. ... Comprehensive assay for the molecular profiling of cancer by target enrichment from formalin-fixed paraffin-embedded specimens ... Comprehensive assay for the molecular profiling of cancer by target enrichment from formalin-fixed paraffin-embedded specimens ... Comprehensive assay for the molecular profiling of cancer by target enrichment from formalin-fixed paraffin-embedded specimens ...
https://okayama.pure.elsevier.com/en/publications/comprehensive-assay-for-the-molecular-profiling-of-cancer-by-targ
IMSEAR at SEARO: Detection and identification of Mycobacterium species by polymerase chain reaction (PCR) from paraffin...
MATERIAL AND METHOD: Paraffin-embedded tissue blocks, the corresponding histopathologic slides, and cultural results were ... Detection and identification of Mycobacterium species by polymerase chain reaction (PCR) from paraffin-embedded tissue compare ... from paraffin-embedded tissue compare to AFB staining in pathological sections. Journal of the Medical Association of Thailand ... to determine the possibility of using PCR technique as an alternative way to detect mycobacterial DNA from paraffin-embedded ...
https://imsear.searo.who.int/handle/123456789/38659
Detection of Leishmania infection in paraffin-embedded skin biopsies of dogs using polymerase chain reaction<...Detection of Leishmania infection in paraffin-embedded skin biopsies of dogs using polymerase chain reaction<...
Roura, X., Fondevila, D., Sánchez, A., & Ferrer, L. (1999). Detection of Leishmania infection in paraffin-embedded skin ... Roura, X, Fondevila, D, Sánchez, A & Ferrer, L 1999, Detection of Leishmania infection in paraffin-embedded skin biopsies of ... Detection of Leishmania infection in paraffin-embedded skin biopsies of dogs using polymerase chain reaction. In: Journal of ... Detection of Leishmania infection in paraffin-embedded skin biopsies of dogs using polymerase chain reaction. / Roura, Xavier; ...
https://portalrecerca.uab.cat/en/publications/detection-of-leishmania-infection-in-paraffin-embedded-skin-biops
Histological, immunohistochemical and mRNA gene expression responses in coeliac disease patients challenged with gluten using...Histological, immunohistochemical and mRNA gene expression responses in coeliac disease patients challenged with gluten using...
... were successful in PAXgene fixed paraffin-embedded samples. Messenger RNA extraction from the same paraffin-embedded biopsy ... In addition, the diagnostically valuable IgA deposits were now stained in paraffin-embedded specimens making them more ... expression changes in coeliac disease patients challenged with gluten using PAXgene fixed paraffin-embedded biopsies. Fifteen ... measured histologic and molecular markers suitable for gluten challenge studies can be obtained from a single paraffin-embedded ...
https://bmcgastroenterol.biomedcentral.com/articles/10.1186/s12876-019-1089-7
Image: Salmon poisoning disease, small intestine, dog - Merck Veterinary ManualImage: Salmon poisoning disease, small intestine, dog - Merck Veterinary Manual
Paraffin-embedded section, H & E staining.. Courtesy of Dr. Yasuko Rikihisa.. In these topics ...
https://www.merckvetmanual.com/multimedia/image/salmon-poisoning-disease-small-intestine-dog
Recombinant Anti-CD13 antibody [EPR4058] KO Tested (ab108310) | AbcamRecombinant Anti-CD13 antibody [EPR4058] KO Tested (ab108310) | Abcam
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD13 antibody [EPR4058]. Excellent ... Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD13 antibody [EPR4058]. Excellent ... Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD13 antibody [EPR4058]. Excellent ... Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD13 antibody [EPR4058]. Excellent ...
https://www.abcam.com/cd13-antibody-epr4058-ab108310.html
FFPESpecimensTissuesImmunohistochemistryExtracted from formalin fixed paraffin embeddedBiopsyHistopathologicFixed in formalinSlidesSamplesHistologyBiopsiesDetectionTumor sectionsSectionsRoutinePolymeraseFlow cytometryProteinsCarcinomaSquamousImmunohistochemicalFrozen tissueBlockIncubateProstate cancerAdding the tissueProtocolBoneDilutionStainInterferenceCorrelation
FFPE13
  • We here characterize the usability of archival formalin-fixed paraffin-embedded (FFPE) brain tissue as a resource for genetic and DNA methylation analyses with potential relevance for brain-manifested diseases. (diagenode.com)
  • Recent advances in next-generation sequencing have led to the increased use of formalin-fixed and paraffin-embedded (FFPE) tissues for medical samples in disease and scientific research. (pacb.com)
  • The data presented here demonstrates single molecule sequencing of DNA samples extracted from tissues embedded in FFPE. (pacb.com)
  • Here, we describe a transcriptomic profiling analysis of matched formalin-fixed and paraffin-embedded (FFPE) DCIS and IBC components of individual breast tumours, containing both tumour compartments. (biomedcentral.com)
  • Formalin -fixed paraffin -embedded (FFPE) tissue archives in hospitals , biobanks, and others offer a vast collection of extensive, readily available specimens for molecular testing . (bvsalud.org)
  • However, SBH cannot be performed with small biopsy or formalin-fixed paraffin-embedded (FFPE) tissue samples because this assay requires a large amount of DNA without degradation. (nature.com)
  • It is as such impossible to perform SBH with small biopsy or formalin-fixed paraffin-embedded (FFPE) samples, which are commonly used for pathological diagnoses. (nature.com)
  • Next-generation sequencing (NGS) testing of formalin-fixed, paraffin-embedded (FFPE) tissues is widelyused in clinical diagnosis. (genopeaks.com)
  • Beside peripheral blood specimen, Formalin-Fixed Paraffin-Embedded (FFPE) marrow specimen can be used for detection of this mutation. (iium.edu.my)
  • We evaluated the performance of circulating cell-free DNA (cfDNA) and formalin-fixed paraffin-embedded (FFPE) tissue DNA analyses using a commonly employed targeted therapeutic pathway in predicting the outcomes of patients with lung cancer, a common cancer with a generally poor prognosis. (clinmedjournals.org)
  • and Exon 20: H1047L, H1047R, H1047Y) using genomic DNA (gDNA) extracted from formalin-fixed, paraffin-embedded (FFPE) breast tumor tissue or circulating tumor DNA (ctDNA) from plasma derived from K2EDTA anticoagulated peripheral whole blood taken from patients with breast cancer. (fda.gov)
  • A total of 42 formalin-fixed, paraffin-embedded (FFPE) tumour samples were available for nanostring based-multigene Assay. (jcancer.org)
  • Cryosections, rather than formalin-fixed paraffin embedded (FFPE) sections, are preferred because the recovery of quality RNA from FFPE sections can be quite challenging. (cdc.gov)
Specimens9
  • Plugs of histologically identified tissue from benign and tumor areas were sampled from paraffin blocks of prostate biopsy specimens by using a 4-mm skin biopsy punch. (aacrjournals.org)
  • Flow cytometric analysis of cellular DNA content in paraffin wax-embedded specimens of canine mammary tumours / E. Scanziani, M. Caniatti, S. Sen, E. Erba, F. Cairoli, M. Battocchio. (unimi.it)
  • In addition, the diagnostically valuable IgA deposits were now stained in paraffin-embedded specimens making them more accessible in routine clinics. (biomedcentral.com)
  • Specimens containing calcium cannot be cut after paraffin embedding. (aruplab.com)
  • The infiltrating of tissue specimens with paraffin, as a supporting substance, to prepare for sectioning with a microtome. (bvsalud.org)
  • All gallbladder cells specimens had been surgically obtained set in 10% buffered formalin and paraffin-embedded. (cancer-ecosystem.com)
  • The skin specimens were paraffin-embedded slides. (cdc.gov)
  • Routine histopathologic study of paraffin-embedded specimens from these tumors suggested the diagnosis of lymphoma or reticulum-cell sarcoma. (jamanetwork.com)
  • Protocol for the detection of Treponema pallidum in paraffin-embedded specimens. (cdc.gov)
Tissues13
  • In conclusion, formalin-fixed paraffin-embedded biopsy tissues are a viable source of miRNA-sequencing material. (nih.gov)
  • Mutation screening using formalin-fixed paraffin-embedded tissues: a stratified approach according to DNA quality. (cam.ac.uk)
  • DNA samples from formalin-fixed paraffin-embedded tissues are highly degraded with variable quality, and this imposes a big challenge for targeted sequencing due to false positives, largely caused by PCR errors and cytosine deamination. (cam.ac.uk)
  • If available, we highly recommend that unprocessed tissues in 10% neutral buffered formalin be submitted in addition to paraffin blocks. (cdc.gov)
  • After fixation, if not paraffin-embedded, tissues SHOULD be transferred to 70% ethanol for long term storage and for shipping. (cdc.gov)
  • Epoxy-embedded tissues are also accepted. (cdc.gov)
  • However, we believe, as also others (Niedenberger and Geyer, 2018), that the tissue quality and cellular morphology are much well-preserved in paraffin-embedded tissues. (durrell2012.com)
  • The Siemens Tissue Preparation Solution allows molecular pathology laboratories to integrate samples from both fresh frozen and formalin-fixed, paraffin-embedded tissues into the same workflow, automating the standardized isolation of nucleic acids. (clpmag.com)
  • Immunohistochemical stains are available for detecting HHV-6 in formalin-fixed paraffin-embedded tissues. (medscape.com)
  • Tissues were embedded in paraffin, epoxy (EponAraldite), and butoxyethanol-glycol methacrylate (BGMA). (cdc.gov)
  • A retrospective study was carried out on 66 formalin-fixed, paraffin-embedded tissues with simple endometrial hyperplasia (31 cases) and endometrial cancer (35 cases) patients. (koreamed.org)
  • Detection of mycobacteria in paraffin-embedded Ziehl-Neelsen-Stained tissues using digital pathology. (bvsalud.org)
  • RV202 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues, and flow cytometry. (biod.co.kr)
Immunohistochemistry8
  • What is paraffin immunohistochemistry? (durrell2012.com)
  • How do you make a paraffin section for immunohistochemistry? (durrell2012.com)
  • Immunohistochemistry (IHC) Protocol-Paraffin Section Protocol. (durrell2012.com)
  • IHC Antibodies Immunohistochemistry(IHC) Protocol Prepare formalin-fixed, paraffin-embedded tissue sections (Step 1-8): 1. (durrell2012.com)
  • Immunohistochemistry on a formalin-fixed, paraffin-embedded section of human placenta showing positive staining in connective tissue cells and no reactivity in epithelial cells. (biod.co.kr)
  • Immunohistochemistry on a formalin-fixed, paraffin-embedded section of human tonsillar lymphoma. (biod.co.kr)
  • Our dedicated histopathology lab contains all the necessary equipment for paraffin or resin-embedding, sectioning, staining, immunohistochemistry and analysis. (cbset.org)
  • Immunohistochemistry involved removal of the reported prevalence rates of byssinosis of approximate- paraffin wax from the sections ( 10 ). (who.int)
Extracted from formalin fixed paraffin embedded1
  • METHODS: NGS libraries are generated from DNA extracted from formalin fixed paraffin embedded tumors. (jax.org)
Biopsy5
  • MicroRNAs (miRNAs) have been increasingly investigated for their predictive value, and formalin-fixed paraffin-embedded biopsy archives may potentially be a valuable source of miRNA sequencing material, as they remain an underused resource. (nih.gov)
  • Messenger RNA extraction from the same paraffin-embedded biopsy block was successful and allowed large-scale qRT-PCR and RNAseq analyses for gene expression. (biomedcentral.com)
  • Rigorous digitally measured histologic and molecular markers suitable for gluten challenge studies can be obtained from a single paraffin-embedded biopsy specimen. (biomedcentral.com)
  • The authors tried to determine the possibility of using PCR technique as an alternative way to detect mycobacterial DNA from paraffin-embedded tissue to avoid repeated biopsy from the patient. (who.int)
  • Hematoxylin and eosin (H&E)‐stained 3-5‐μm sections are cut from tissue obtained with a core biopsy, and are typically fixed in formalin, processed, and embedded in a paraffin block. (radiologykey.com)
Histopathologic2
  • MATERIAL AND METHOD: Paraffin-embedded tissue blocks, the corresponding histopathologic slides, and cultural results were retrospectively searched for according to the patient's records, the granuloma clinic, Department of Dermatology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand from 1994-2000. (who.int)
  • As fixed and paraffin embedded for histopathologic analysis. (cdc.gov)
Fixed in formalin1
  • Organ samples were fixed in formalin, embedded in paraffin, sectioned, and stained with hematoxylin and eosin. (cdc.gov)
Slides3
  • glass slides, paraffin-embedded tissue). (collegerecruiter.com)
  • Formalin-fixed, paraffin embedded tissue block or cell block, or 1 unstained charged slide for each test requested plus 3-4 additional unstained slides cut at 4 microns. (cellnetix.com)
  • Formalin-fixed, paraffin-embedded tissue sections were used to perform in situ hybridization using newly available probe sets (Ventana Medical Systems, Tucson, Ariz). The slides were examined for the presence of integrated HPV using light microscopy. (duke.edu)
Samples10
  • Previously, such techniques were limited by the fact that fresh tissue was required for analysis, but recent studies have demonstrated that a sufficient amount of RNA can be extracted from paraffin-embedded samples. (oncolink.org)
  • 89 of the Milan patients' paraffin-embedded samples were analyzable. (oncolink.org)
  • Flow cytometric analysis of DNA content was performed on 72 formalin-fixed, paraffin wax-embedded samples of canine mammary tumours. (unimi.it)
  • Conclusion: Dilution of the DNA extracted of paraffin-embedded materials did not modify statistically the amount of positive samples β-globin gene amplified in PCR, although the results suggest that this is a way to increase the method for efficacy amplification of PCR. (bvsalud.org)
  • Stainings for γδ T cells and IgA deposits, where previously frozen samples have been needed, were successful in PAXgene fixed paraffin-embedded samples. (biomedcentral.com)
  • Formalin -fixed paraffin -embedded tissue samples were collected and archived from autopsies conducted over 13 years in the forensic medicine department of the University of Pretoria (Pretoria, South Africa ). (bvsalud.org)
  • This process involves orienting warm tissue samples into hot paraffin wax molds, which are then cooled until the paraffin block is solid. (aruplab.com)
  • Embedding instructions, provided by the client, guide lab staff on how to orient the tissue samples so that the area of interest is sectioned correctly. (aruplab.com)
  • Samples of human IVD tissue were fixed in neutral buffered formalin, embedded in paraffin, and sections stained with hematoxylin and eosin to score the degree of morphological degeneration. (shu.ac.uk)
  • Paraffin blocks of the sputum samples were made of respiratory symptoms, and have reported longitudinal as described previously ( 10 ). (who.int)
Histology1
  • Heavy metal staining of paraffin epoxy and glycol methacrylate embedded biological tissue for scanning electron microscope histology. (cdc.gov)
Biopsies4
  • Immunohistochemical detection of cell-cycle associated markers on paraffin embedded and formalin fixed needle biopsies of prostate cancer: correlation of p120 protein expression with AgNOR, PCNA/Cyclin, KI-67/MIB1 proliferation-scores and Gleason gradings. (unimore.it)
  • Paraffin embedded and formalin fixed needle biopsies of prostate cancer (PC) were used to immunocytochemically detect the p120 nucleolar protein in relation to the Gleason histological gradings (GHG), the labelling indices of proliferating nuclear immunocytochemical markers (PCNA/Cyclin, Ki-67/MIB1) and the argyrophilic nucleolar region (AgNOR) rate. (unimore.it)
  • In conclusion, paraffin embedded and formalin fixed PC needle biopsies exhibit a higher diagnostic PCNA/Cyclin than Ki-67/MIB1 index for cases presenting differentiated features, whereas p120 nucleolar protein detection seems to be a suitable marker of poorer outcome of PC. (unimore.it)
  • We here evaluated morphometric, immunohistochemical and messenger RNA (mRNA) expression changes in coeliac disease patients challenged with gluten using PAXgene fixed paraffin-embedded biopsies. (biomedcentral.com)
Detection2
  • Our objective in this study was to determine the optimal antigen retrieval (AR) protocol for detection of TSP in formalin-fixed, paraffin-embedded tissue by using tissue sections from patients with invasive transitional cell carcinoma of the bladder. (wustl.edu)
  • IMSEAR at SEARO: Detection and identification of Mycobacterium species by polymerase chain reaction (PCR) from paraffin-embedded tissue compare to AFB staining in pathological sections. (who.int)
Tumor sections2
  • DNA from paraffin-embedded tumor sections was analyzed using microdissection, direct sequencing analysis and allelic separation by cloning. (uniroma5.it)
  • Cases were appropriately staged, and paraffin-embedded tumor sections, stained with hematoxylin and eosin, were graded. (jomfp.in)
Sections13
  • Can you do immunofluorescence on paraffin embedded sections? (durrell2012.com)
  • The technique can be applied for other tissue types, both paraffin-embedded sections and cryosections. (durrell2012.com)
  • How thick are paraffin embedded sections that has been cut? (durrell2012.com)
  • How are paraffin sections prepared in the lab? (durrell2012.com)
  • Paraffin sections were stained with silver. (cdc.gov)
  • Paraffin and BGMA sections were histologically stained for LM. (cdc.gov)
  • Cytometric DNA analysis was performed on formalin-fixed paraffin embedded sections from 36 Iraqi patients with oral squamous cell carcinoma. (who.int)
  • Antigen retrieval by BD Pharmingen Retrievagen A (pH 6.0): Formalin-fixed paraffin sections of normal human tonsil were either untreated (left panel) or pre-treated with BD Retrievagen A (pH 6.0) (right panel) nd then stained with the anti-Ki-67 antibody. (bdbiosciences.com)
  • BD Retrievagen A (pH 6.0) is an antigen retrieval system that is recommended for Zinc-fixed and Formalin-fixed paraffin embedded tissue sections that show sub-optimal or no reactivity with antibodies for immunohistochemical staining. (bdbiosciences.com)
  • It is important to realize that in spite of antigen retrieval, some antibodies do not stain on formalin-fixed paraffin embedded sections. (bdbiosciences.com)
  • Additional reported applications (for the relevant formats) include: immunoprecipitation 1-3 , complement-dependent cytotoxicity 4 , in vivo and in vitro blocking of adhesion 1-3,5 , and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections 6 . (biolegend.com)
  • This antibody is designed for the specific localization of human calcitonin using IHC techniques in formalin-fixed, paraffin-embedded tissue sections. (anacrom.com)
  • Sections (5 µm thick) of changes in the pulmonary function test as annual decline the paraffin blocks were cut using a microtome, and in lung capacity, in addition to chest tightness, chronic stained with immunohistochemical molecular markers bronchitis, and chronic cough, and sharp decline in forced for epithelial membrane antigen (EMA) or cytokeratin expiratory volume in one second ( 2 ). (who.int)
Routine1
  • Tissue-Wax is a highly purified, filtered, white paraffin wax blended with synthetic polymers and suitable for routine embedding and sectioning applications. (thelabwarehouse.com)
Polymerase1
  • Introduction: Several reasons may lead to the failure of polymerase chain reaction (PCR) using DNA purified from paraffin-embedded materials: presence of inhibitors and degradation of target DNA. (bvsalud.org)
Flow cytometry1
  • Methods to disintegrate old paraffin-embedded tissue blocks for the application of DNA flow cytometry open up new possibilities for retrospective studies on the correlation between tumor cell nuclear DNA pattern and prognosis of the neoplastic disease. (aacrjournals.org)
Proteins1
  • N 1 PFA/PBS overnight, and Calcitonin Proteins Recombinant Proteins paraffin-embedded. (calcium-channel.com)
Carcinoma1
  • The assay is intended for laboratory use to detect the anaplastic lymphoma kinase protein in formalin-fixed, paraffin-embedded, non-small cell lung carcinoma tissue stained using the Ventana BenchMark XT or Ventana BenchMark Ultra automated immunohistochemical slide strainers. (clpmag.com)
Squamous1
  • Material and Method: Paraffin-embedded blocks from 30 patients with oropharynx squamous cell carcinomas, diagnosed and treated at the Oral Oncology Center were selected. (bvsalud.org)
Immunohistochemical1
  • Our results demonstrate that TSP can be reliably detected in paraffin-embedded tissue by immunohistochemical techniques that utilize AR with high-temperature microwave heating and a low-pH Tris-HCl buffer. (wustl.edu)
Frozen tissue1
  • Receives and accessions various types of biospecimens including fresh blood and bone marrow, frozen tissue, frozen biological liquids, and paraffin materials (ex. (collegerecruiter.com)
Block2
  • Pour melted paraffin into paraffin block mold. (durrell2012.com)
  • Submit a formalin-fixed, paraffin-embedded tissue block to be cut and returned. (testcatalog.org)
Incubate1
  • Incubate the tissue in a 65 °C paraffin bath for 2 times, 30 min each. (durrell2012.com)
Prostate cancer1
  • IHC image of CSB-RA987782A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. (ewmccall.com)
Adding the tissue1
Protocol3
  • Sectioning Protocol: Section paraffin blocks at the desired thickness (usually 4-5 µm) on a microtome and float on a 40°C water bath containing distilled water. (durrell2012.com)
  • What is the IHC protocol for paraffin? (durrell2012.com)
  • This automated protocol involves running tissue through a vacuum infiltration processing machine, which replaces all water present in the tissue with paraffin wax to stabilize the tissue and make it amenable to cutting. (aruplab.com)
Bone2
  • All tissue containing calcium (bone, bone marrow, etc.) must be submerged in a decal solution prior to processing and embedding until tissue is pliable. (aruplab.com)
  • Tissue containing calcium must undergo calcium removal before embedding unless specific studies requiring undecalcified bone are requested. (aruplab.com)
Dilution1
  • Objective: To evaluate the dilution influence of DNA purified from paraffin-embedded materials on β-globin PCR amplification. (bvsalud.org)
Stain1
Interference1
  • Epoxy embedding - for the best intracellular details and tissue patterns, without interference from grid bars. (cdc.gov)
Correlation2
  • Paraffin embedding - for the best correlation of surface topography and internal structure. (cdc.gov)
  • BGMA embedding - for the best correlation of LM, SEM and TEM. (cdc.gov)
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