ONCOGENE PROTEINS from papillomavirus that deregulate the CELL CYCLE of infected cells and lead to NEOPLASTIC CELL TRANSFORMATION. Papillomavirus E7 proteins have been shown to interact with various regulators of the cell cycle including RETINOBLASTOMA PROTEIN and certain cyclin-dependent kinase inhibitors.
Neoplasms of the skin and mucous membranes caused by papillomaviruses. They are usually benign but some have a high risk for malignant progression.
A species of DELTAPAPILLOMAVIRUS infecting cattle.
Products of viral oncogenes, most commonly retroviral oncogenes. They usually have transforming and often protein kinase activities.
A family of small, non-enveloped DNA viruses infecting birds and most mammals, especially humans. They are grouped into multiple genera, but the viruses are highly host-species specific and tissue-restricted. They are commonly divided into hundreds of papillomavirus "types", each with specific gene function and gene control regions, despite sequence homology. Human papillomaviruses are found in the genera ALPHAPAPILLOMAVIRUS; BETAPAPILLOMAVIRUS; GAMMAPAPILLOMAVIRUS; and MUPAPILLOMAVIRUS.
A type of ALPHAPAPILLOMAVIRUS especially associated with malignant tumors of the CERVIX and the RESPIRATORY MUCOSA.
A type of ALPHAPAPILLOMAVIRUS causing recurrent respiratory PAPILLOMATOSIS; GENITAL WARTS; and other neoplasms.
Vaccines or candidate vaccines used to prevent PAPILLOMAVIRUS INFECTIONS. Human vaccines are intended to reduce the incidence of UTERINE CERVICAL NEOPLASMS, so they are sometimes considered a type of CANCER VACCINES. They are often composed of CAPSID PROTEINS, especially L1 protein, from various types of ALPHAPAPILLOMAVIRUS.
A type of human papillomavirus especially associated with malignant tumors of the genital and RESPIRATORY MUCOSA.
Tumors or cancer of the UTERINE CERVIX.
The type species of KAPPAPAPILLOMAVIRUS. It is reported to occur naturally in cottontail rabbits in North America.
A type of ALPHAPAPILLOMAVIRUS usually associated with GENITAL WARTS; and LARYNGEAL NEOPLASMS.
Infections produced by oncogenic viruses. The infections caused by DNA viruses are less numerous but more diverse than those caused by the RNA oncogenic viruses.
Deoxyribonucleic acid that makes up the genetic material of viruses.
A type of XIPAPILLOMAVIRUS causing alimentary carcinoma in cattle. It is related to Bovine papillomavirus 3.
Proteins found in any species of virus.
A genus of DNA viruses in the family PAPILLOMAVIRIDAE. They preferentially infect the anogenital and ORAL MUCOSA in humans and primates, causing both malignant and benign neoplasms. Cutaneous lesions are also seen.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
A circumscribed benign epithelial tumor projecting from the surrounding surface; more precisely, a benign epithelial neoplasm consisting of villous or arborescent outgrowths of fibrovascular stroma covered by neoplastic cells. (Stedman, 25th ed)
Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.
A malignancy arising in uterine cervical epithelium and confined thereto, representing a continuum of histological changes ranging from well-differentiated CIN 1 (formerly, mild dysplasia) to severe dysplasia/carcinoma in situ, CIN 3. The lesion arises at the squamocolumnar cell junction at the transformation zone of the endocervical canal, with a variable tendency to develop invasive epidermoid carcinoma, a tendency that is enhanced by concomitant human papillomaviral infection. (Segen, Dictionary of Modern Medicine, 1992)
Benign epidermal proliferations or tumors; some are viral in origin.
An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus.
Sexually transmitted form of anogenital warty growth caused by the human papillomaviruses.
A type of ALPHAPAPILLOMAVIRUS associated with high risk for anogenital neoplasms.
The neck portion of the UTERUS between the lower isthmus and the VAGINA forming the cervical canal.
Collection of pooled secretions of the posterior vaginal fornix for cytologic examination.
Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.
A genus of DNA viruses in the family PAPILLOMAVIRIDAE causing fibropapillomas in their respective ungulate hosts. Species infected include cattle, European elk, deer, and sheep.
DNA probes specific for the identification of human papilloma virus.
Established cell cultures that have the potential to propagate indefinitely.
Pathological processes of the UTERINE CERVIX.
Product of the retinoblastoma tumor suppressor gene. It is a nuclear phosphoprotein hypothesized to normally act as an inhibitor of cell proliferation. Rb protein is absent in retinoblastoma cell lines. It also has been shown to form complexes with the adenovirus E1A protein, the SV40 T antigen, and the human papilloma virus E7 protein.
A genus of DNA viruses in the family PAPILLOMAVIRIDAE, causing cutaneous lesions in humans. Infections exist in latent form in the general population and are activated under conditions of IMMUNOSUPPRESSION.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Nuclear phosphoprotein encoded by the p53 gene (GENES, P53) whose normal function is to control CELL PROLIFERATION and APOPTOSIS. A mutant or absent p53 protein has been found in LEUKEMIA; OSTEOSARCOMA; LUNG CANCER; and COLORECTAL CANCER.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
A PDGF receptor that binds specifically to the PDGF-B chain. It contains a protein-tyrosine kinase activity that is involved in SIGNAL TRANSDUCTION.
The functional hereditary units of VIRUSES.
The process by which a DNA molecule is duplicated.
Methods for detecting or typing the DNA of an ALPHAPAPILLOMAVIRUS in biological tissues and fluids.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Abnormal development of immature squamous EPITHELIAL CELLS of the UTERINE CERVIX, a term used to describe premalignant cytological changes in the cervical EPITHELIUM. These atypical cells do not penetrate the epithelial BASEMENT MEMBRANE.
Proteins that form the CAPSID of VIRUSES.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
Cancers or tumors of the PENIS or of its component tissues.
Cytological preparation of cells collected from a mucosal surface and stained with Papanicolaou stain.
Tumors or cancer of the VULVA.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.
A carcinoma derived from stratified SQUAMOUS EPITHELIAL CELLS. It may also occur in sites where glandular or columnar epithelium is normally present. (From Stedman, 25th ed)
The complete genetic complement contained in a DNA or RNA molecule in a virus.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Tumors or cancer of the OROPHARYNX.
Tumors or cancer of the ANAL CANAL.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.
The examination, therapy or surgery of the cervix and vagina by means of a specially designed endoscope introduced vaginally.
An autosomal recessive trait with impaired cell-mediated immunity. About 15 human papillomaviruses are implicated in associated infection, four of which lead to skin neoplasms. The disease begins in childhood with red papules and later spreads over the body as gray or yellow scales.
A genus of DNA viruses in the family PAPILLOMAVIRIDAE, which cause cutaneous lesions in humans. They are histologically distinguishable by intracytoplasmic INCLUSION BODIES which are species specific.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Genes whose gain-of-function alterations lead to NEOPLASTIC CELL TRANSFORMATION. They include, for example, genes for activators or stimulators of CELL PROLIFERATION such as growth factors, growth factor receptors, protein kinases, signal transducers, nuclear phosphoproteins, and transcription factors. A prefix of "v-" before oncogene symbols indicates oncogenes captured and transmitted by RETROVIRUSES; the prefix "c-" before the gene symbol of an oncogene indicates it is the cellular homolog (PROTO-ONCOGENES) of a v-oncogene.
Tumors or cancer of the VAGINA.
Tumors or cancer of the PALATINE TONSIL.
Proteins that catalyze the unwinding of duplex DNA during replication by binding cooperatively to single-stranded regions of DNA or to short regions of duplex DNA that are undergoing transient opening. In addition DNA helicases are DNA-dependent ATPases that harness the free energy of ATP hydrolysis to translocate DNA strands.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Specific receptors on cell membranes that react with PLATELET-DERIVED GROWTH FACTOR, its analogs, or antagonists. The alpha PDGF receptor (RECEPTOR, PLATELET-DERIVED GROWTH FACTOR ALPHA) and the beta PDGF receptor (RECEPTOR, PLATELET-DERIVED GROWTH FACTOR BETA) are the two principle types of PDGF receptors. Activation of the protein-tyrosine kinase activity of the receptors occurs by ligand-induced dimerization or heterodimerization of PDGF receptor types.
A diverse class of enzymes that interact with UBIQUITIN-CONJUGATING ENZYMES and ubiquitination-specific protein substrates. Each member of this enzyme group has its own distinct specificity for a substrate and ubiquitin-conjugating enzyme. Ubiquitin-protein ligases exist as both monomeric proteins multiprotein complexes.
Proteins coded by oncogenes. They include proteins resulting from the fusion of an oncogene and another gene (ONCOGENE PROTEINS, FUSION).
Protein interaction domains of about 70-90 amino acid residues, named after a common structure found in PSD-95, Discs Large, and Zona Occludens 1 proteins. PDZ domains are involved in the recruitment and interaction of proteins, and aid the formation of protein scaffolds and signaling networks. This is achieved by sequence-specific binding between a PDZ domain in one protein and a PDZ motif in another protein.
Immunoglobulins produced in response to VIRAL ANTIGENS.
A unique DNA sequence of a replicon at which DNA REPLICATION is initiated and proceeds bidirectionally or unidirectionally. It contains the sites where the first separation of the complementary strands occurs, a primer RNA is synthesized, and the switch from primer RNA to DNA synthesis takes place. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Viral diseases which are transmitted or propagated by sexual conduct.
Tumor or cancer of the MALE GENITALIA.
Proteins transcribed from the E1A genome region of ADENOVIRUSES which are involved in positive regulation of transcription of the early genes of host infection.
Tumor suppressor genes located on human chromosome 13 in the region 13q14 and coding for a family of phosphoproteins with molecular weights ranging from 104 kDa to 115 kDa. One copy of the wild-type Rb gene is necessary for normal retinal development. Loss or inactivation of both alleles at this locus results in retinoblastoma.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
The study of the structure, growth, function, genetics, and reproduction of viruses, and VIRUS DISEASES.
A 1.5-kDa small ubiquitin-related modifier protein that can covalently bind via an isopeptide link to a number of cellular proteins. It may play a role in intracellular protein transport and a number of other cellular processes.
Skin diseases caused by viruses.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
A family of proteins that are structurally-related to Ubiquitin. Ubiquitins and ubiquitin-like proteins participate in diverse cellular functions, such as protein degradation and HEAT-SHOCK RESPONSE, by conjugation to other proteins.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
A lesion with cytological characteristics associated with invasive carcinoma but the tumor cells are confined to the epithelium of origin, without invasion of the basement membrane.
A transcription factor that possesses DNA-binding and E2F-binding domains but lacks a transcriptional activation domain. It is a binding partner for E2F TRANSCRIPTION FACTORS and enhances the DNA binding and transactivation function of the DP-E2F complex.
The total number of cases of a given disease in a specified population at a designated time. It is differentiated from INCIDENCE, which refers to the number of new cases in the population at a given time.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
A family of basic helix-loop-helix transcription factors that control expression of a variety of GENES involved in CELL CYCLE regulation. E2F transcription factors typically form heterodimeric complexes with TRANSCRIPTION FACTOR DP1 or transcription factor DP2, and they have N-terminal DNA binding and dimerization domains. E2F transcription factors can act as mediators of transcriptional repression or transcriptional activation.
A large family of regulatory proteins that function as accessory subunits to a variety of CYCLIN-DEPENDENT KINASES. They generally function as ENZYME ACTIVATORS that drive the CELL CYCLE through transitions between phases. A subset of cyclins may also function as transcriptional regulators.
Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.
A large multisubunit complex that plays an important role in the degradation of most of the cytosolic and nuclear proteins in eukaryotic cells. It contains a 700-kDa catalytic sub-complex and two 700-kDa regulatory sub-complexes. The complex digests ubiquitinated proteins and protein activated via ornithine decarboxylase antizyme.
An essential ribonucleoprotein reverse transcriptase that adds telomeric DNA to the ends of eukaryotic CHROMOSOMES.
The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.
Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
Suspensions of attenuated or killed viruses administered for the prevention or treatment of infectious viral disease.
A cyclin-dependent kinase inhibitor that mediates TUMOR SUPPRESSOR PROTEIN P53-dependent CELL CYCLE arrest. p21 interacts with a range of CYCLIN-DEPENDENT KINASES and associates with PROLIFERATING CELL NUCLEAR ANTIGEN and CASPASE 3.
The outer protein protective shell of a virus, which protects the viral nucleic acid.
A persistent progressive non-elevated red scaly or crusted plaque which is due to an intradermal carcinoma and is potentially malignant. Atypical squamous cells proliferate through the whole thickness of the epidermis. The lesions may occur anywhere on the skin surface or on mucosal surfaces. The cause most frequently found is trivalent arsenic compounds. Freezing, cauterization or diathermy coagulation is often effective. (From Rook et al., Textbook of Dermatology, 4th ed, pp2428-9)
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
Methods for determining interaction between PROTEINS.
Tumor suppressor genes located on the short arm of human chromosome 17 and coding for the phosphoprotein p53.
Pathological processes that tend eventually to become malignant. (From Dorland, 27th ed)
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
The naturally occurring or experimentally induced replacement of one or more AMINO ACIDS in a protein with another. If a functionally equivalent amino acid is substituted, the protein may retain wild-type activity. Substitution may also diminish, enhance, or eliminate protein function. Experimentally induced substitution is often used to study enzyme activities and binding site properties.
A ubiquitously expressed regulatory protein that contains a retinoblastoma protein binding domain and an AT-rich interactive domain. The protein may play a role in recruiting HISTONE DEACETYLASES to the site of RETINOBLASTOMA PROTEIN-containing transcriptional repressor complexes.
Administration of vaccines to stimulate the host's immune response. This includes any preparation intended for active immunological prophylaxis.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
The interactions between a host and a pathogen, usually resulting in disease.
Biochemical identification of mutational changes in a nucleotide sequence.
The decrease in the cell's ability to proliferate with the passing of time. Each cell is programmed for a certain number of cell divisions and at the end of that time proliferation halts. The cell enters a quiescent state after which it experiences CELL DEATH via the process of APOPTOSIS.
Recombinant DNA vectors encoding antigens administered for the prevention or treatment of disease. The host cells take up the DNA, express the antigen, and present it to the immune system in a manner similar to that which would occur during natural infection. This induces humoral and cellular immune responses against the encoded antigens. The vector is called naked DNA because there is no need for complex formulations or delivery agents; the plasmid is injected in saline or other buffers.
A family of histone acetyltransferases that is structurally-related to CREB-BINDING PROTEIN and to E1A-ASSOCIATED P300 PROTEIN. They function as transcriptional coactivators by bridging between DNA-binding TRANSCRIPTION FACTORS and the basal transcription machinery. They also modify transcription factors and CHROMATIN through ACETYLATION.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
Paxillin is a signal transducing adaptor protein that localizes to FOCAL ADHESIONS via its four LIM domains. It undergoes PHOSPHORYLATION in response to integrin-mediated CELL ADHESION, and interacts with a variety of proteins including VINCULIN; FOCAL ADHESION KINASE; PROTO-ONCOGENE PROTEIN PP60(C-SRC); and PROTO-ONCOGENE PROTEIN C-CRK.
Sexual activities of humans.
Neoplasms of the SQUAMOUS EPITHELIAL CELLS. The concept does not refer to neoplasms located in tissue composed of squamous elements.
Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.
Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS.
Organized periodic procedures performed on large groups of people for the purpose of detecting disease.
Curved rows of HAIR located on the upper edges of the eye sockets.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.
An aspect of personal behavior or lifestyle, environmental exposure, or inborn or inherited characteristic, which, on the basis of epidemiologic evidence, is known to be associated with a health-related condition considered important to prevent.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.
Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
The process by which two molecules of the same chemical composition form a condensation product or polymer.
Proteins prepared by recombinant DNA technology.
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.
Cancers or tumors of the LARYNX or any of its parts: the GLOTTIS; EPIGLOTTIS; LARYNGEAL CARTILAGES; LARYNGEAL MUSCLES; and VOCAL CORDS.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
An E3 UBIQUITIN LIGASE that interacts with and inhibits TUMOR SUPPRESSOR PROTEIN P53. Its ability to ubiquitinate p53 is regulated by TUMOR SUPPRESSOR PROTEIN P14ARF.
Tumor or cancer of the female reproductive tract (GENITALIA, FEMALE).
The terminal segment of the LARGE INTESTINE, beginning from the ampulla of the RECTUM and ending at the anus.
MOLECULAR BIOLOGY techniques used in the diagnosis of disease.
A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.
The genital canal in the female, extending from the UTERUS to the VULVA. (Stedman, 25th ed)
The external reproductive organ of males. It is composed of a mass of erectile tissue enclosed in three cylindrical fibrous compartments. Two of the three compartments, the corpus cavernosa, are placed side-by-side along the upper part of the organ. The third compartment below, the corpus spongiosum, houses the urethra.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
Inflammation of the UTERINE CERVIX.
Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. A commonly observed structure may be composed of a CONSERVED SEQUENCE which can be represented by a CONSENSUS SEQUENCE.
A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).
Tumors or cancer of the SKIN.
Protein kinases that control cell cycle progression in all eukaryotes and require physical association with CYCLINS to achieve full enzymatic activity. Cyclin-dependent kinases are regulated by phosphorylation and dephosphorylation events.
A cell line derived from cultured tumor cells.
Pathological processes involving the male reproductive tract (GENITALIA, MALE).
A class of enzymes that catalyze the formation of a bond between two substrate molecules, coupled with the hydrolysis of a pyrophosphate bond in ATP or a similar energy donor. (Dorland, 28th ed) EC 6.
Methods used to study CELLS.
A transferase that catalyzes the addition of aliphatic, aromatic, or heterocyclic FREE RADICALS as well as EPOXIDES and arene oxides to GLUTATHIONE. Addition takes place at the SULFUR. It also catalyzes the reduction of polyol nitrate by glutathione to polyol and nitrite.
Pathological processes involving the PENIS or its component tissues.
Diagnosis of the type and, when feasible, the cause of a pathologic process by means of microscopic study of cells in an exudate or other form of body fluid. (Stedman, 26th ed)
A genus of DNA viruses in the family papillomaviridae, causing mucosal and cutaneous lesions in cats and dogs. Canine oral papillomavirus is the type species.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
Deletion of sequences of nucleic acids from the genetic material of an individual.
A product of the p16 tumor suppressor gene (GENES, P16). It is also called INK4 or INK4A because it is the prototype member of the INK4 CYCLIN-DEPENDENT KINASE INHIBITORS. This protein is produced from the alpha mRNA transcript of the p16 gene. The other gene product, produced from the alternatively spliced beta transcript, is TUMOR SUPPRESSOR PROTEIN P14ARF. Both p16 gene products have tumor suppressor functions.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Systems of enzymes which function sequentially by catalyzing consecutive reactions linked by common metabolic intermediates. They may involve simply a transfer of water molecules or hydrogen atoms and may be associated with large supramolecular structures such as MITOCHONDRIA or RIBOSOMES.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Soft tissue tumors or cancer arising from the mucosal surfaces of the LIP; oral cavity; PHARYNX; LARYNX; and cervical esophagus. Other sites included are the NOSE and PARANASAL SINUSES; SALIVARY GLANDS; THYROID GLAND and PARATHYROID GLANDS; and MELANOMA and non-melanoma skin cancers of the head and neck. (from Holland et al., Cancer Medicine, 4th ed, p1651)
Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.
Tumors or cancer of the MOUTH.
A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.
Pathological processes involving the female reproductive tract (GENITALIA, FEMALE).
In a prokaryotic cell or in the nucleus of a eukaryotic cell, a structure consisting of or containing DNA which carries the genetic information essential to the cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
Ribonucleic acid that makes up the genetic material of viruses.
ENDOPEPTIDASES which have a cysteine involved in the catalytic process. This group of enzymes is inactivated by CYSTEINE PROTEINASE INHIBITORS such as CYSTATINS and SULFHYDRYL REAGENTS.
Lining of the ORAL CAVITY, including mucosa on the GUMS; the PALATE; the LIP; the CHEEK; floor of the mouth; and other structures. The mucosa is generally a nonkeratinized stratified squamous EPITHELIUM covering muscle, bone, or glands but can show varying degree of keratinization at specific locations.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
Methods to identify and characterize cancer in the early stages of disease and predict tumor behavior.
Insertion of viral DNA into host-cell DNA. This includes integration of phage DNA into bacterial DNA; (LYSOGENY); to form a PROPHAGE or integration of retroviral DNA into cellular DNA to form a PROVIRUS.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Substances elaborated by viruses that have antigenic activity.
A condition of the mucous membrane characterized by erythematous papular lesions. (Dorland, 27th ed)
Excision of the prepuce of the penis (FORESKIN) or part of it.
Proteins that are normally involved in holding cellular growth in check. Deficiencies or abnormalities in these proteins may lead to unregulated cell growth and tumor development.
The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.

Concordant induction of cyclin E and p21cip1 in differentiated keratinocytes by the human papillomavirus E7 protein inhibits cellular and viral DNA synthesis. (1/899)

Productive infections by human papillomaviruses (HPVs) occur only in differentiated keratinocytes in squamous epithelia in which the HPV E7 protein reactivates the host DNA replication machinery to support viral DNA replication. In a fraction of the differentiated keratinocytes, E7 also posttranscriptionally induces p21Cip1, which is distributed in a mutually exclusive manner with unscheduled cellular DNA synthesis. In this study, double immunofluorescence labeling unexpectedly revealed that E7 caused a concordant accumulation of both cyclin E and p21Cip1 to high levels in patient papillomas and in organotypic cultures of primary human keratinocytes. The induction of cyclin E is mutually exclusive with unscheduled cellular DNA synthesis or abundant viral DNA. These novel virus-host interactions in differentiated keratinocytes are in contrast to previous observations made in submerged proliferating cultures, in which HPV E7 induces cyclin E and overcomes p21Cip1 inhibition of S-phase entry. We propose that an appropriately timed induction of cyclin E/cyclin-dependent kinase 2 by HPV E7 in postmitotic cells enables S-phase reentry and HPV DNA amplification, whereas prematurely induced cyclin E stabilizes p21Cip1 protein, which then inhibits cyclin E/cyclin-dependent kinase 2. Consequently, cyclin E and p21Cip1 both fail to turn over, and DNA synthesis does not occur.  (+info)

Kinetic selection of HPV 16 E6/E7-directed antisense nucleic acids: anti-proliferative effects on HPV 16-transformed cells. (2/899)

The E6/E7-coding sequences of the human papillomavirus type 16 (HPV 16) were probed for kinetic accessibility in vitro by pools of catalytic antisense RNA. Only long-chain complementary RNA and very few antisense sequences with a 3' portion complementary to a 10 nt window within unspliced and spliced E6-coding target sequences showed fast annealing with k(ass) values of up to 10(4) M-1s-1 indicating that the majority of E6/E7 RNA sequences are unfavourable targets for antisense inhibitors and ribozymes. Fast-annealing antisense oligodeoxyribonucleotides directed against the window of 10 nt inhibited cell proliferation of HPV 16-transformed SiHa cells but not slow-annealing antisense species. Antisense RNA of several hundred nucleotides in length also showed significant anti-proliferative activity. Biological effects of antisense oligodeoxyribonucleotides were specific for the antisense sequence, could only be found in HPV-positive but not in HPV-negative cell lines, and were related to decreased levels of E7 protein and E6/E7-specific transcripts. This work suggests that HPV 16 E7/E6 sequences exhibit a low accessibility for antisense oligonucleotides. This can be overcome, however, by exploiting the relationship between fast annealing of antisense species and their increased efficacy in human cells.  (+info)

Induction of tumor antigen-specific immunity using plasmid DNA immunization in mice. (3/899)

We have evaluated the ability of bioballistic "gene gun" immunization of mice with plasmid DNA encoding clinically relevant tumor antigens to induce protective antitumor immunity. Mice immunized with plasmid cDNA encoding the cervical carcinoma-associated human papillomavirus 16-E7 gene product exhibited potent anti-E7-specific cytotoxic T lymphocytes and were protected completely against a subsequent challenge with the E7+ C3 sarcoma. Of perhaps greater clinical interest, genetic immunization using cDNA encoding the normal, germline-encoded murine melanosomal protein tyrosinase-related protein-2 (TRP-2) resulted in delayed outgrowth of TRP-2+ B16 melanoma in mice and was associated with an in vivo activation of TRP-2-specific cytotoxic T lymphocytes. Codelivery of plasmid cDNA encoding TRP-2 and the T helper 1-biasing cytokine murine interleukin-12 considerably enhanced the antitumor efficacy of these gene-based melanoma vaccines.  (+info)

Synergism with germ line transcription factor Oct-4: viral oncoproteins share the ability to mimic a stem cell-specific activity. (4/899)

Activation of transcription by Oct-4 from remote binding sites requires a cofactor that is restricted to embryonal stem cells. The adenovirus E1A protein can mimic the activity of this stem cell-specific factor and stimulates Oct-4 activity in differentiated cells. Here we characterize the Oct-4-E1A interaction and show that the E1A 289R protein harbors two independent Oct-4 binding sites, both of which specifically interact with the POU domain of Oct-4. Furthermore, we demonstrate that, like E1A, the human papillomavirus E7 oncoprotein also specifically binds to the Oct-4 POU domain. E7 and Oct-4 can form a complex both in vitro and in vivo. Expression of E7 in differentiated cells stimulates Oct-4-mediated transactivation from distal binding sites. Moreover, Oct-4, but not other Oct factors, is active when expressed in cells transformed by human papillomavirus. Our results suggest that different viruses have evolved oncoproteins that share the ability to target Oct-4 and to mimic a stem cell-specific activity.  (+info)

Control of replicative life span in human cells: barriers to clonal expansion intermediate between M1 senescence and M2 crisis. (5/899)

The accumulation of genetic abnormalities in a developing tumor is driven, at least in part, by the need to overcome inherent restraints on the replicative life span of human cells, two of which-senescence (M1) and crisis (M2)-have been well characterized. Here we describe additional barriers to clonal expansion (Mint) intermediate between M1 and M2, revealed by abrogation of tumor-suppressor gene (TSG) pathways by individual human papillomavirus type 16 (HPV16) proteins. In human fibroblasts, abrogation of p53 function by HPVE6 allowed escape from M1, followed up to 20 population doublings (PD) later by a second viable proliferation arrest state, MintE6, closely resembling M1. This occurred despite abrogation of p21(WAF1) induction but was associated with and potentially mediated by a further approximately 3-fold increase in p16(INK4a) expression compared to its level at M1. Expression of HPVE7, which targets pRb (and p21(WAF1)), also permitted clonal expansion, but this was limited predominantly by increasing cell death, resulting in a MintE7 phenotype similar to M2 but occurring after fewer PD. This was associated with, and at least partly due to, an increase in nuclear p53 content and activity, not seen in younger cells expressing E7. In a different cell type, thyroid epithelium, E7 also allowed clonal expansion terminating in a similar state to MintE7 in fibroblasts. In contrast, however, there was no evidence for a p53-regulated pathway; E6 was without effect, and the increases in p21(WAF1) expression at M1 and MintE7 were p53 independent. These data provide a model for clonal evolution by successive TSG inactivation and suggest that cell type diversity in life span regulation may determine the pattern of gene mutation in the corresponding tumors.  (+info)

Overexpression of D-type cyclins, E2F-1, SV40 large T antigen and HPV16 E7 rescue cell cycle arrest of tsBN462 cells caused by the CCG1/TAF(II)250 mutation. (6/899)

tsBN462 cells, which have a point mutation in CCG1/TAF(II)250, a component of TFIID complex, arrest in G1 at the nonpermissive temperature of 39.5 degrees C. Overexpression of D-type cyclins rescued the cell cycle arrest of tsBN462 cells, suggesting that the cell cycle arrest was through Rb. Consistent with this, overexpression of E2F-1, whose function is repressed by the hypophosphorylated form of Rb, also rescued the cell cycle arrest. Moreover, expression of the viral oncoproteins SV40 large T antigen and HPV16 E7, which both bind Rb and inactivate its function, rescued the cell cycle arrest, whereas HPV16 E6 did not. Mutation of the Rb-binding motif in E7 abrogated its ability to rescue the cell cycle arrest. Expression of exogenous cyclin D1, SV40 large T antigen or CCG1/TAF(II)250 increased cyclin A expression at 39.5 degrees C. Coexpression of HPV16 E7 and adenovirus E1b19K, which blocks apoptosis, rescued the proliferation of tsBN462 cells at 38.5 degrees C. To investigate the mechanism underlying the lack of cyclin D1 expression, deletion analysis of cyclin D1 promoter was performed. The 0.15 kbp cyclin D1 core promoter region, which lacks any transcription factor binding motifs, still exhibited a temperature-sensitive phenotype in tsBN462 cells suggesting that CCG1/TAF(II)250 is critical for the function of the cyclin D1 core promoter.  (+info)

Induction of specific CD8+ T-lymphocyte responses using a human papillomavirus-16 E6/E7 fusion protein and autologous dendritic cells. (7/899)

When intracellular viral proteins are degraded, only a limited number of peptide epitopes are capable of eliciting specific CD8+ cellular immune responses for a given human leukocyte antigen (HLA) haplotype. We sought to induce CD8+ T-lymphocyte (CTL) responses to human papillomavirus-16 (HPV-16) E6 and E7 proteins using a recombinant E6/E7 fusion protein and autologous human dendritic cells (DCs). CTLs were generated by in vitro stimulation using a recombinant HPV-16 E6/E7 fusion protein and autologous DCs from a healthy HLA-A*0201 donor. CTL specificity was assessed by cytokine release assays when the cells were reacted with autologous DC targets coincubated with the E6/E7 fusion protein. These CTLs were also reacted with the immunodominant E7 peptides (E711-20 and E7(86-93)) and DCs as a target. As a negative control, DCs were incubated with or without an irrelevant control protein (Helicobacter pylori) as target for the E6/E7-induced CTLs. The E6/E7-induced CTLs were capable of specific recognition of target DCs coincubated with E6/E7 but not the control protein. When E6/E7-specific CTLs were reacted with DCs and either E7(11-20) or E7(86-93), specific peptide recognition was also detected. These data demonstrate that specific CTLs can be elicited using autologous human DCs and a HPV-16 E6/E7 fusion protein. Therefore, extracellular viral proteins seem to be engulfed and processed by DCs; then the immunodominant HLA-A2-restricted peptides become available for CD8+ T-lymphocyte recognition. These data suggest that vaccine strategies using recombinant viral proteins may overcome the limitation of peptide epitopes for specific HLA haplotypes and may, therefore, permit more generalized clinical application.  (+info)

Expression, purification and immunological characterization of the transforming protein E7, from cervical cancer-associated human papillomavirus type 16. (8/899)

E7 is the major oncogenic protein produced in cervical cancer-associated human papillomavirus type 16 (HPV16). This protein was expressed in Escherichia coli as a glutathione-S-transferase (GST) fusion protein. E7-enriched inclusion bodies were collected from bacterial lysates, were solubilized in 10 M urea, and the protein was purified using anion exchange column chromatography. After removal of endotoxin with serial Triton X-114 extractions, material of high purity (about 90%) was obtained, which is suitable for use in a human clinical trial. This material was immunogenic, and when used as a vaccine, protected mice against challenge with an HPV16 E7 DNA transfected tumour cell line. Based on this observation, the E7GST fusion protein is currently being used in a human clinical trial of a vaccine against HPV16-induced cervical cancer. This fusion protein could be cleaved with thrombin to remove the GST fusion part and further purified by preparative SDS gel electrophoresis to obtain free E7 with > 98% purity.  (+info)

The laboratory is studying the structure and function of human and viral oncoproteins with a goal to develop small molecule inhibitors as molecular probes and as lead molecules for development to treat various cancers. There is a particular interest in melanoma and the laboratory had developed inhibitors to several important oncoprotein targets in melanoma including BRAF, PI3K and PAK1. The laboratory is also targeting the oncoproteins E7 and E6 from human papillomavirus (HPV). HPV is known to be the causative agent of a number of epithelial cancers, most notably cervical cancer, and has also been implicated to have a causative role in about 20% of head and neck cancers as well as several other cancers. We have recently reported on the development of potent and selective HPV-E7 inhibitors, while the development of HPV-E6 inhibitors is in progress. The laboratory is also studying the structure and function of the tumor suppressor targets of HPV-E7 and -E6, pRb and p53, respectively ...
Oncotarget | https://doi.org/10.18632/oncotarget.11810 Dingqing Feng, Keqin Yan, Ying Zhou, Haiyan Liang, Jing Liang, Weidong Zhao, Zhongjun Dong, Bin Ling
BioAssay record AID 411957 submitted by ChEMBL: Induction of apoptosis in human CaSki cells assessed as proteolytic activation of caspase 3 at 1 mM by SDS-PAGE.
CONICET Digital, el repositorio institucional del CONICET, un servicio gratuito para acceder a la producción científico-tecnológica de investigadores, becarios y demás personal del CONICET.
We have sequenced 1730 bp of human papilloma virus type 18 (HPV 18) DNA containing the open reading frames (ORF) E6, E7, the N-terminal part of E1 and, additionally, 120 bp of the N-terminal part of L1. Based on these sequencing data, together with the human papilloma virus type 16 (HPV 16) DNA sequence published recently, we identified and cloned the ORF E6, E7, E1 and L1 of HPV 18 and the ORF E6, E7, E1, E4, E5, L2 and L1 of HPV 16 into prokaryotic expression vectors. The expression system used provides fusions to the N-terminal part of the MS2 polymerase gene controlled by the heat-inducible lambda PL promoter. Using the purified fusion proteins as immunogens we raised antisera against the proteins encoded by the ORF E6, E7 and E1 of HPV 18 as well as those encoded by the ORF E6, E7, E4 and L1 of HPV 16. By Western blot analysis we could show that the E7 gene product is the most abundant protein in cell lines containing HPV 16 or HPV 18 DNA. It is a cytoplasmic protein of 15 kd in the SiHa and the
Human papillomaviruses (HPVs) are the primary causative agents for cervical cancer, and HPV oncoproteins E6 and E7 are known to be the main reason for the onset and maintenance of the malignancies. Therefore, inhibition of viral E6 and E7 oncoproteins expression represents a viable strategy to cervical cancer therapies. This study is to evaluate the antiviral effect of a novel N-Phenylbenzamide derivative, 3-(2-Chloropropyl amide)-4-methoxy-N-phenylbenzamide (L17), against HPV16 in vitro and identify its associated mechanism of action in cervical cancer cells. The cytotoxic effect of L17 was assessed by MTT assay. The mRNA and protein levels of E6 and E7 oncogenes were analyzed by quantitative real-time reverse transcription PCR (qRT-PCR) and Western blot, respectively. p53 and Rb protein levels were also detected by Western blot. The effect of L17 on cell cycle was analyzed by flow cytometry. The cytotoxic effect of L17 was greater in cervical carcinoma cells than in normal cells. L17 significantly
Human papillomaviruses (HPVs) are the primary causative agents for cervical cancer, and HPV oncoproteins E6 and E7 are known to be the main reason for the onset and maintenance of the malignancies. Therefore, inhibition of viral E6 and E7 oncoproteins expression represents a viable strategy to cervical cancer therapies. This study is to evaluate the antiviral effect of a novel N-Phenylbenzamide derivative, 3-(2-Chloropropyl amide)-4-methoxy-N-phenylbenzamide (L17), against HPV16 in vitro and identify its associated mechanism of action in cervical cancer cells. The cytotoxic effect of L17 was assessed by MTT assay. The mRNA and protein levels of E6 and E7 oncogenes were analyzed by quantitative real-time reverse transcription PCR (qRT-PCR) and Western blot, respectively. p53 and Rb protein levels were also detected by Western blot. The effect of L17 on cell cycle was analyzed by flow cytometry. The cytotoxic effect of L17 was greater in cervical carcinoma cells than in normal cells. L17 significantly
Recent next-generation sequencing studies have generated a comprehensive overview of the genomic landscape of human papillomavirus (HPV)-associated cancers. This review summarizes these findings to provide insight into the tumor biology of these cancers and potential therapeutic opportunities for HPV-driven malignancies. In addition to the tumorigenic properties of the HPV oncoproteins, integration of HPV DNA into the host genome is suggested to be a driver of the neoplastic process. Integration may confer a growth and survival advantage via enhanced expression of viral oncoproteins, alteration of critical cellular genes, and changes in global promoter methylation and transcription. Alteration of cellular genes may lead to loss of function of tumor suppressor genes, enhanced oncogene expression, loss of function of DNA repair genes, or other vital cellular functions. Recurrent integrations in RAD51B, NR4A2, and TP63, leading to aberrant forms of these proteins, are observed in both HPV-positive ...
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TY - JOUR. T1 - Enhancing DNA vaccine potency by combining a strategy to prolong dendritic cell life and intracellular targeting strategies with a strategy to boost CD4+ T cell. AU - Kim, Daejin. AU - Hoory, Talia. AU - Wu, T. C.. AU - Hung, Chien Fu. PY - 2007/11/1. Y1 - 2007/11/1. N2 - Intradermal administration of DNA vaccines, using a gene gun, represents an effective means of delivering DNA directly into professional antigen-presenting cells (APCs) in the skin and thus allows the application of strategies to modify the properties of APCs to enhance DNA vaccine potency. In the current study, we hypothesized that the potency of human papillomavirus (HPV) type 16 E7 DNA vaccines employing intracellular targeting strategies combined with a strategy to prolong the life of dendritic cells (DCs) could be further enhanced by the addition of a DNA vaccine capable of generating high numbers of pan-HLA-DR reactive epitope (PADRE)-specific CD4+ T cells. We observed that the addition of PADRE DNA to E7 ...
Intra-tumor injection of lentiviral-vector delivered shRNA targeting human papillomavirus E6 and E7 oncogenes reduces tumor growth in a xenograft cervical cancer model in mice
In this study we show that the HPV16 E7-specific CD8+ T cell response is far more vigorous after vaccination with a HPV16 E7-derived 35-residue long peptide than following vaccination with the minimal CTL epitope. Our data suggest that two distinct DC-related mechanisms lie at the basis of this result. First, the long peptide contains both a CTL epitope and a Th epitope. Prime-boost vaccinations of wild-type, MHC class II−/−, and CD40−/− mice show that the CD40-CD40L interactions between APC and E7-specific Th cells contribute considerably to the level of the CD8+ T cell response. Second, under circumstances excluding T cell help, a comparison of the CD8+ T cell responses induced by either the minimal CTL epitope (nine residues) or the long peptide combined with DC-activating agents demonstrates that vaccination with the long peptide results in far better responses. This suggests that compared with the short minimal CTL epitope the long peptide is much better presented by professional ...
CTLs specific for high-risk human papillomaviruses (HPVs) have recently been found in the peripheral blood of cervical cancer patients. Although cell-mediated immunity is thought to be important in the control of HPV infection, the functional relevance and site of activation of HPV-specific CTLs are unclear. We identified HLA-A*0201-restricted HPV-16 E7 peptide-specific CTLs in the peripheral blood (four of five patients), draining lymph nodes (three of four patients) and tumors (one of three patients) of cervical cancer patients. In four of four cancer patients, the frequency of CTLs specific for a recombinant vaccinia virus expressing HPV-16 and -18 E6/E7 gene products was found to be higher in tumors and lymph nodes compared with that of peripheral blood. HPV-specific CTLs were not identified in any of seven healthy controls, but primary responses could be generated by peptide-pulsed dendritic cells (four of four controls). In a non-HLA-A*0201 subject with invasive carcinoma, other HLA ...
La raison pour laquelle ta gynéco en a parlé p- e, c est que les femmes qui veulent tomber enceinte doivent souvent passer un papillomavirus tomber enceinte d infection de chlamydia et autre, c est une contrôle, c est normal. Cette bactérie est responsable de l urétrite à Chlamydia ou chlamydiosemaladie sexuellement transmissible qui est la plus fréquente en Amérique latine et aux États- Unis après le Papillomavirus 50 papillomavirus empeche de tomber enceinte plus fréquente que la gonorrhée, elle- même plus fréquente avoir le papillomavirus enceinte la syphilis.
Orplatna devait être papillomavirus traitement alternatif remede naturel contre le papillomavirus naturel pour traiter le cancer de la remede naturel contre le papillomavirus. Traducerile vulgare sau colocviale sunt în general marcate cu roșu sau cu portocaliu. Specialistul gastroenterolog Silva Cesar Pedrazas a acceptat invitația noastră și ne- a explicat, pe înțelesul tuturor, cât de importantă este colonoscopia remede papillomavirus homme sănătatea noastră.
HPV has been linked with an increased risk of cardiovascular disease. Papillomavirus humain oncogene, Non hodgkins lymphoma cancer Comprimate antiparazitare de la demodex Anumite tulpini infectează colul uterin și au fost implicate ca o cauză a cancerului de col uterin HPV. In faza IE sunt activate un numar foarte restans de gene virale IE, care vor codifica sinteza unor proteine virale functionale ce vor activa transcrierea genelor E.
Nu sunt selectate sau validate papillomavirus homme verrue, așadar pot conține termeni inadecvați. Ce este pre-cancer si ce inseamna CIN?
[196 Pages Report] Check for Discount on Global Papillomavirus Diagnostics Market Country Shares and Segment Forecasts 2017-2021: Emerging Opportunities and Growth Strategies for Suppliers report by Venture Planning Group. The report presents a detailed analysis of the Papillomavirus diagnostics...
Din pacate aceste forme nu determina semne sau simptome decat in stadii tardive ale cancerelor respective, ceea ce le face sa fie dificil de tratat si vindecat.
Un alt cancer produs de infectia cu HPV este cancerul vaginal care papillomavirus femme transmission cel mai des la femeile de peste 60 de ani. Cnd este superficial este de culoare roie-albstrie; localizarea profund este pe bulbi sau corpii cavernoi clitoridieni.
În plus, infecțiile cu HPV 16 și 18 sunt o cauză a unui tip unic de cancer orofaringian. Anumite tulpini infectează colul uterin și au fost implicate ca o cauză a cancerului de col uterin HPV.
Parce que de nombreux organismes sont situés dans le bassin, la chirurgie pour enlever les tumeurs de grande taille nécessitent la dépose de ces organes.
L hépatite B est provoquée par un virus qui s attaque au foie. Atunci îi putem mulțumi iubitului tău pentru asta. Cest linterrogatoire du patient qui permet dorienter le diagnostic.
Human papillomaviruses: shared and distinct pathways for pathogenesis. De cele mai multe ori, diagnosticul este pus cu ușurință, bazându-se pe caracteristicile clinice.
Human papillomavirus type 11 L1 protein, Human papillomavirus type 16 L1 protein, Human papillomavirus type 18 L1 protein, Human papillomavirus type 31 L1 protein, Human papillomavirus type 33 L1 protein, Human papillomavirus type 45 L1 protein, Human papillomavirus type 52 L1 protein, Human papillomavirus type 6 L1 protein, Human papillomavirus type 58 L1 ...
Normal human foreskin keratinocytes cotransfected with the neomycin resistance gene and recombinant human papillomavirus (HPV) DNAs (types 16, 18, 31, and 33) that have a high or moderate association with cervical malignancy acquired immortality and contained integrated and transcriptionally active viral genomes. Only transcripts from the intact E6 and E7 genes were detected in at least one cell line, suggesting that one or both of these genes are responsible for immortalization. Recombinant HPV DNAs with low or no oncogenic potential for cervical cancer (HPV1a, -5, -6b, and -11) induced small G418-resistant colonies that senesced as did the nontransfected cells. These colonies contained only episomal virus DNA; therefore, integration of HPV sequences is important for immortalization of keratinocytes. This study suggests that the virus-encoded immortalization function contributes to the pathogenesis of cervical carcinoma. ...
TY - JOUR. T1 - Expression of the human papillomavirus type 11 E5A protein from the [email protected]?E4,E5 transcript. AU - Brown, Darron R.. AU - McClowry, Tracy L.. AU - Sidner, Richard A.. AU - Fife, Kenneth H.. AU - Bryan, Jonine T.. PY - 1998/8/1. Y1 - 1998/8/1. N2 - The abundant human papillomavirus type II (HPV 11) [email protected]?E4,E5 transcript potentially encodes the [email protected]?E4,E5a and E5b proteins. It is not known if either of the E5 proteins are expressed from this transcript. For HPV 16, E5 is a single open reading frame (ORF), and the E5 protein is expressed from an unspliced E2,E5 transcript but not from tile spliced [email protected]?E4,E5 transcript. This study was undertaken to determine if the HPV 11 E5a protein is expressed from the [email protected]?E4,E5 transcript. To detect E5a expression in eukaryotic cells, the green fluorescent protein (GFP) gene was fused to the [email protected]? end of the E5a gene in the pEGFP-N1 vector. Several recombinant plasmid constructs were made to determine if E5a translation is influenced by upstream ...
Summary The L1 and L2 capsid proteins encoded by human papillomavirus types 6 and 16 (HPV-6 and HPV-16) have been synthesized in bacteria. Antisera were raised against the HPV-6 L1- and L2-β-galactosidase fusion proteins and against and HPV-16 L1 C-terminal peptide which was 14 amino acids long. The HPV-16 L1 peptide antibodies have been shown to be highly reactive with the HPV-16 L1-β-galactosidase fusion protein but not against the equivalent HPV-6 L1-β-galactosidase fusion protein. The effectiveness of these antibodies was compared with commercially available antibovine papillomavirus type 1 (BPV-1) antibodies and the results demonstrated that the anti-BPV-1 antibodies reacted well against HPV-6 L1-β-galactosidase but not against HPV-16 L1-β-galactosidase. In addition, the L2 portion of the HPV-6 L2-β-galactosidase fusion protein appeared particularly immunogenic, since antibodies raised against this fusion protein were predominantly reactive with the L2 moiety. The HPV-16L1 peptide antibodies
High-risk human papillomavirus type 16 (HPV16) is a risk factor for cervical cancer. Previous studies suggest that polymorphisms in the E6 gene or the long control region(LCR)of HPV16 may alter the oncogenic potential of the virus. The aims of this study were to investigate the genetic variations of HPV16 E6 gene and LCR in isolates from Chinese population and correlation of the E6 and LCR polymorphisms with disease status of infected patients. HPV16 positive endocervical specimens were collected from 304 women living in Northeast of China. Sequences of E6 gene and LCR were analyzed by PCR-sequencing. Two lineages were found in the populations, including EUR lineage and As lineage. Based on the HPV16 prototype, the most frequent variation in the E6 gene was T178A/G (48.7%), followed by mutations of G94A (12.2%) and T350G (9.9%). The rank orders of incidence of E6 variations in amino acid were as follows: D25E (46.3%), L83V (9.9%) and H78Y (4.3%). Nucleotide variations in LCR were found in all the 304
Human Papilloma virus type 16 E6 antibody for WB. Anti-Human Papilloma virus type 16 E6 pAb (GTX132686) is tested in Human papillomavirus samples. 100% Ab-Assurance.
|strong|Mouse anti Human Papillomavirus 18 Oncoprotein E7 antibody, clone 718-15|/strong| recognizes the E7 oncoprotein of human papilloma virus 18. Human papillomavirus (HPV) is a diverse group of DN…
Human papillomavirus (HPV) is causative for a fresh and increasing type of mind and ML314 throat squamous cell carcinomas (HNSCCs). and level of resistance to Path weighed against HPV-negative mind and throat cancer tumor cell lines. All TRAIL-resistant HPV-positive cell lines tested were sensitised to TRAIL-induced cell death by treatment with bortezomib a clinically authorized proteasome inhibitor. Bortezomib-mediated sensitisation to TRAIL was associated with enhanced activation of caspase-8 -9 and -3 elevated membrane expression levels of TRAIL-R2 cytochrome launch and G2/M arrest. Knockdown of caspase-8 significantly blocked cell death induced from the combination therapy whereas the BH3-only protein Bid was not required for induction of apoptosis. XIAP depletion improved the level of sensitivity of both HPV-positive and -bad cells to TRAIL only or in combination with bortezomib. In contrast repair of p53 following E6 knockdown in HPV-positive cells experienced no effect on their level of ...
TY - JOUR. T1 - Selective activation of cervical microvascular endothelial cells by human papillomavirus 16-E7 oncoprotein. AU - DAnna, Rossella. AU - Le Buanec, Hélène. AU - Alessandri, Giulio. AU - Caruso, Arnaldo. AU - Burny, Arsène. AU - Gallo, Robert. AU - Zagury, Jean François. AU - Zagury, Daniel. AU - DAlessio, Patrizia. PY - 2001/12/19. Y1 - 2001/12/19. N2 - Background: Human papillomavirus type 16 (HPV16) is strongly implicated in the etiology of cervical cancer, with the expression of HPV16-encoded E7 oncoprotein in infected epithelial cells contributing to their malignant transformation. Although nuclear E7 interacts with several nuclear targets, we have previously shown that extracellular E7 can cause suppression of immune cell function. Moreover, cervical microvascular endothelial (CrMVEn) cells treated with E7 increase their expression of adhesion molecules. High levels of some cytokines in serum and in cervicovaginal secretions are associated with the progression of ...
Human papillomavirus E1 and E2 proteins are viral replication factors, E2 being also involved in transcriptional repression of the two viral oncogenes E6 and E7. For HPV 16, the E4 protein is involved in late stages of the viral cycle, has no start codon and is known to be exclusively expressed as an E1^E4 fusion product resulting from alternative splicing linking the beginning of the E1 open reading frame (ORF) to E4. The E4 gene lies within the E2 ORF but in a different frame, and we show here that the HPV18 E2 ORF also encodes two new E2^E4 chimeric proteins produced by alternative splicing of the E2 transcripts between the N-terminal part of E2 and E4. Both E2^E4 proteins are cytoplasmic, one being localized at mitochondria where E1^E4 has been previously shown to localize. In order to study E2 functions independently of E2^E4, we constructed an HPV18 E2 expression vector lacking the splice acceptor site in front of E4 and thus defective in E2^E4 expression. We could then show using ...
Specific types of human papillomaviruses (HPVs) cause cervical cancer. The viral E6 oncogene is a critical factor for maintaining the malignant phenotype of HPV-positive tumour cells. By yeast two-hyb
DNA damage, such as that elicited by UV-B, can induce either a cell cycle arrest or apoptosis that can be signalled by the p53 protein through the activation of a number of downstream cellular target genes. In contrast to oncogenic anogenital human papillomaviruses (HPVs), which mediate proteolytic degradation of p53, the E6 protein of cutaneous HPVs, such as HPV 77, do not promote p53 degradation. We have previously shown, however, that expression of HPV 77 E6 can effectively block UV-induced apoptosis in cells that have UV-activated p53. Here, we report that expression of the E6 protein from the cutaneous HPV 77 attenuates the UV-induced transactivation of p53-regulated proapoptotic genes Fas, PUMAbeta, Apaf-1, PIG3. This inhibition of p53-activation of proapoptotic genes by HPV77 E6 is exerted selectively, as the increased expression of p53 target genes involved in cell cycle arrest or regulatory functions regulation, such as p21 and Hdm2, is unaffected. Our data suggest that HPV 77 E6 may ...
Sigma-Aldrich offers abstracts and full-text articles by [Stephanie van de Wall, Mateusz Walczak, Nienke van Rooij, Baukje-Nynke Hoogeboom, Tjarko Meijerhof, Hans W Nijman, Toos Daemen].
Procedee de indepartare a verucilor negilor Exista medicamente topice care se aplica local, pe leziune care pot trata leziunile vizibile, dar papillomavirus obicei este papillomavirus de mai multe aplicari, inainte de a elimina cu suces veruca sau verucile. Nu s - a observat nicio tendinţă clară în funcţie de vârstă.
臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。. To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of NTU Repository with Academic Hub to form NTU Scholars.. ...
Nu a existat o diferenţă semnificativă statistic, privind riscul unei infecţii cu HPV cu o nouă tulpină, între pacienţii HPV seropozitivi şi cei HPV seronegativi, lucru care a fost valabil atât pentru femeile infectate HIV, cât şi pentru cele neinfectate, pentru toate tipurile testate, cu excepţia HPV Jumtate din toate tumorile de 3, 8, 10 - flat warts. Pe baza datelor privind imunogenitatea, obţinute din studiile clinice, regimul original de vaccinare cu trei doze a fost înlocuit cu două doze pentru indivizii papillomavirus in females cu vârsta sub 15 ani, în
Opens the Highlight Feature Bar and highlights feature annotations from the FEATURES table of the record. The Highlight Feature Bar can be used to navigate to and highlight other features and provides links to display the highlighted region separately. Links in the FEATURES table will also highlight the corresponding region of the sequence. More... ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Baby Boomers Face Risk of HPV-Related Throat Cancer - Cedars-Sinai papillomavirus age Din fericire, virusul dispare în timp în unele situații, însă doar în cazurile în care sistemul imunitar este suficient de puternic și poate lupta împotriva acestuia.
Papillomaviridae Papillomaviridae Papillomaviridae este o veche familie taxonomică a virusurilor ADN ne-învelite, denumite în mod colectiv papilomavirusuri. Apasă pentru a vedea traducerea automată a definiției în Română. Cnd sunt zgriate ele pot sngera.
MmuPV1, a papillomavirus that infects laboratory mice (Mus musculus), is discovered to be sexually transmitted, providing a new animal virus model to study sexually transmitted human papillomaviruses (HPVs).
Human papillomaviruses (HPV) have been implicated in the pathogenesis of several cancers of the anogenital tract, including cervical, vulvar, vaginal, and anal cancers in women and penile and anal can
Journal of Medical Virology Christine Mant, Barbara Kell, Philip Rice, Jennifer M. Best, Jon M. Bible, John Cason Article first published online: 6 OCT 2003 DOI: 10.1002/jmv.10529 Keywords: HPV-16; children; transient exposure Abstract High-risk human papillomaviruses, such as type 16 (HPV-16), are established etiological agents for cervical carcinoma. In most cases, this virus is transmitted sexually ...
The activity and epithelial tropism of the human papillomavirus type 18 P105 early promoter, which directs the synthesis of the E6 and E7 transforming genes, are controlled by cis elements included in the viral long control region. To identify potential cellular regulators of this promoter, we mutagenized one or both of the 5-TGACTAA-3 cis elements capable of interacting with the AP1 transcription factor, which is composed either of homodimers or heterodimers of the Jun products or of heterodimers of Jun and Fos. Mutation of both elements completely abolished P105 promoter activity in human keratinocytes. We show that either AP1 site can interact efficiently in vitro with any of the three different Jun products as heterodimers with c-Fos. However, in nuclear extracts prepared from human keratinocytes, JunB was the predominant Jun component bound to the DNA probe containing this cis element. These results implicate JunB as an important factor in human papillomavirus type 18 transcription in ...
CLINICAL INVESTIGATION (Araflt rma) DIAGNOSING HUMAN PAPILLOMAVIRUS AND HUMAN PAPILLOMAVIRUS TYPE 16 BY REALTIME PCR IN PATIENT UNDERGONE TO COLPOSCOPY AND SIGNIFICANCE OF THE DIAGNOSIS Sibel OZDAS 1,
Cervical carcinoma cells producing high levels of interleukin-6 (IL-6) were shown to be unresponsive to the cytokine IL-6 due to the loss of their IL-6 receptor. Addition of IL-6 receptor in a soluble form restores IL-6 signalling in SW756 carcinoma cells. This leads to a rapid and strong activation of the transcription factor signal transducer and activator of transcription 3 (STAT3). Nuclear factor IL-6 (NF-IL6, C/EBPβ) was induced only as a late event. While C/EBPβ significantly repressed the human papillomavirus type 18 long control region (HPV18-LCR), IL-6 signalling unexpectedly activated the HPV18-LCR in these cells. This IL-6 receptor-mediated induction could be completely reverted by transfection of a dominant-negative STAT3 but not STAT1 expression construct, indicating that STAT3 might play an important role in HPV18 oncogene promoter activation.
TY - JOUR. T1 - The interaction between steroid hormones, human papillomavirus type 16, E6 oncogene expression, and cervical cancer. AU - Moodley, M. AU - Sewart, S. AU - Herrington, C. S. AU - Chetty, R. AU - Pegoraro, R. AU - Moodley, J. PY - 2003. Y1 - 2003. N2 - Various risk factors have been implicated in the causation of cervical cancer including human papillomavirus (HPV), the early genes (E6 and E7 ) of which encode the main transforming proteins. Studies have suggested that steroid hormones may enhance the expression of these genes leading to loss of p53 gene-mediated cell apoptosis. A total of 120 cervical tissue samples were obtained from patients with proven cervical cancer. Patients who used depo-medroxyprogesterone acetate steroid contraception were recruited as part of the steroid arm. Only HPV DNA type 16 samples were used for the study. Controls included three cell lines (CaSki, SiHa, & C33A) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal ...
TY - JOUR. T1 - Molecular cloning, analysis, and chromosomal localization of a mouse genomic sequence related to the human papillomavirus type 18 E5 region. AU - Kahn, Tomas. AU - Friesl, Holger. AU - Copeland, Neal G.. AU - Gilbert, Debra J.. AU - Jenkins, Nancy A.. AU - Gissmann, Lutz. AU - Kramer, Judith. AU - Hausen, Harald Zur. PY - 1992/1/1. Y1 - 1992/1/1. N2 - The E5 open reading frame (ORF) from bovine papillomavirus type 1 (BPV 1) as well as the E5 ORFs from human papillomaviruses (HPV) type 6 and type 16 have been reported to transform immortalized rodent cells. In an analysis of murine and human tumors for the presence of putative papillomavirus-related sequences, we cloned amplified cellular sequences from the mouse cell line Eb that cross-hybridized with the E5 ORF of HPV 18. A 2.1-kb fragment termed HC1 was sequenced. In normal murine cells, it was present as a single-copy genomic sequence located on chromosome 8. A region of 213 nucleotides corresponded to the E5 gene (HC1 E5), ...
TY - JOUR. T1 - Cooperation between Different Forms of the Human Papillomavirus Type 1 E4 Protein To Block Cell Cycle Progression and Cellular DNA Synthesis. AU - Knight, G. L.. AU - Grainger, J. R.. AU - Gallimore, P. H.. AU - Roberts, S.. PY - 2004/12/15. Y1 - 2004/12/15. N2 - Posttranslational modification-oligomerization, phosphorylation, and proteolytic cleavage-of the human papillomavirus (HPV) E4 protein occurs as the infected keratinocytes migrate up through the suprabasal wart layers. It has been postulated that these events modify E4 function during the virus life cycle. In HPV type 1 (HPV1)-induced warts, N-terminal sequences are progressively cleaved from the full-length E4 protein (E1∧E4) of 17 kDa to produce a series of polypeptides of 16, 11 and 10 kDa. Here, we have shown that in human keratinocytes, a truncated protein (E4-16K), equivalent to the 16-kDa species, mediated a G2 arrest in the cell cycle that was dependent on a threonine amino acid in a proline-rich domain of the ...
1. Walboomers JM, Jacobs MV, Manos MM. et al. Human papillomavirus is a necessary cause of invasive cervical cancer worldwide. J Pathol. 1999;189(1):12-9 2. Burd EM. Human papillomavirus and cervical cancer. Clin Microbiol Rev. 2003;16(1):1-17 3. Boyer SN, Wazer DE, Band V. E7 protein of human papilloma virus-16 induces degradation of retinoblastoma protein through the ubiquitin-proteasome pathway. Cancer Res. 1996;56(20):4620-4 4. Thomas M, Pim D, Banks L. The role of the E6-p53 interaction in the molecular pathogenesis of HPV. Oncogene. 1999;18(53):7690-700 5. Thierry F. Transcriptional regulation of the papillomavirus oncogenes by cellular and viral transcription factors in cervical carcinoma. Virology. 2009;384(2):375-9 6. Badaracco G, Venuti A, Sedati A. et al. HPV16 and HPV18 in genital tumors: significantly different levels of viral integration and correlation to tumor invasiveness. J Med Virol. 2002;67(4):574-82 7. Romanczuk H, Howley PM. Disruption of either the E1 or the E2 regulatory ...
We have isolated four clones of integrated human papillomavirus type 16 (HPV-16) DNA from four different primary cervical cancer specimens. All clones were found to be monomeric or dimeric forms of HPV-16 DNA with cellular flanking sequences at both ends. Analysis of the viral sequences in these clones showed that E6/E7 open reading frames and the long control region were conserved and that no region specific for the integration was detected. Analysis of the cellular flanking sequences revealed no significant homology with any known human DNA sequences, except Alu sequences, and no homology among the clones, indicating no cellular sequence specific for the integration. By probing with single-copy cellular flanking sequences from the clones, it was demonstrated that the integrated HPV-16 DNAs, with different sizes in the same specimens, shared the same cellular flanking sequences at the ends. Furthermore, it was shown that the viral sequences together with cellular flanking sequences were ...
Signs of Human Papillomavirus including medical signs and symptoms of Human Papillomavirus, symptoms, misdiagnosis, tests, common medical issues, duration, and the correct diagnosis for Human Papillomavirus signs or Human Papillomavirus symptoms.
TY - JOUR. T1 - Human papillomavirus-16 E7 interacts with Siva-1 and modulates apoptosis in HaCaT human immortalized keratinocytes. AU - Severino, Anna. AU - Abbruzzese, Claudia. AU - Manente, Lucrezia. AU - Valderas, Álvaro Avivar. AU - Mattarocci, Stefano. AU - Federico, Antonio. AU - Starace, Giuseppe. AU - Chersi, Alberto. AU - Mileo, Anna Maria. AU - Paggi, Marco G.. PY - 2007/7. Y1 - 2007/7. N2 - The viral factor E7 plays a key role in the well-established association between high-risk Human Papillomavirus (HPV) infection and the development of epithelial malignant tumors, as uterine cervix and ano-genital cancer. To delve into the molecular mechanisms of HPV-mediated cell transformation, we searched for novel potential cellular targets of the HPV-16 E7 oncoprotein, by means of the yeast two-hybrid technique, identifying a protein-protein interaction between HPV-16 E7 and the pro-apoptotic cellular factor Siva-1. Using co-precipitation assays and the PepSets technique, we confirmed ...
Quantitative human papillomavirus type 16 viral load and prognosis of cervical cancer treatment efficiency is considered in the article.
TY - JOUR. T1 - Human papillomavirus type 16-associated periungeal squamous cell carcinoma in a patient with acquired immunodeficiency syndrome [7]. AU - Tosti, A.. AU - La Placa, M.. AU - Fanti, P. A.. AU - Gentilomi, G.. AU - Venturoli, S.. AU - Zerbini, M.. AU - Musiani, M.. PY - 1994/12/1. Y1 - 1994/12/1. UR - http://www.scopus.com/inward/record.url?scp=0028569606&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0028569606&partnerID=8YFLogxK. M3 - Letter. C2 - 7701891. AN - SCOPUS:0028569606. VL - 74. SP - 478. EP - 479. JO - Acta Dermato-Venereologica. JF - Acta Dermato-Venereologica. SN - 0001-5555. IS - 6. ER - ...
Find the best human papillomavirus hpv doctors in Mumbai. Get guidance from medical experts to select human papillomavirus hpv specialist in Mumbai from trusted hospitals - credihealth.com
Productive infections by human papillomaviruses (HPVs) occur only in differentiated keratinocytes in squamous epithelia in which the HPV E7 protein reactivates the host DNA replication machinery to support viral DNA replication. In a fraction of the differentiated keratinocytes, E7 also posttranscriptionally induces p21cip1, which is distributed in a mutually exclusive manner with unscheduled cellular DNA synthesis. In this study, double immunofluorescence labeling unexpectedly revealed that E7 caused a concordant accumulation of both cyclin E and p21cip1 to high levels in patient papillomas and in organotypic cultures of primary human keratinocytes. The induction of cyclin E is mutually exclusive with unscheduled cellular DNA synthesis or abundant viral DNA. These novel virus-host interactions in differentiated keratinocytes are in contrast to previous observations made in submerged proliferating cultures, in which HPV E7 induces cyclin E and overcomes p21cip1 inhibition of S-phase entry. We ...
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Human papillomavirus (HPV) 16 and HPV 18 E2 amino acid variants and host HLA DR/DQ haplotypes have been identified by direct nucleic acid sequencing from cervical scrapes. HPV16 E2 variants co-segregate with a nucleotide variant at nt350 (in E6), which previously has been associated with persistent …
Cervical cancer the most frequent neoplasm and the third mortality rate of malignancies of the women in the world. It results in about 200,000 women dying of cervical cancer each year worldwide. The available forms of treatment-surgery, radiation therapy, and chemotherapy are all cytoreductive treatment modalities, so in addition to killing cancerous cells, healthy cells are also destroyed in the process. Indeed, there is a need to decrease the incidence of cervical cancer and develop better forms of its treatment. Human papilloma viruses (HPV) have been consistently implicated in causing cervical cancer especially those high-risk types (HPV 16,18,31,45) have been strongly associated with cervical cancer. HPV 16 was found in more than 50% of cervical cancer tissues. So the host immune response plays an important role in determining the regression of cervical abnormality or persistence and progression to malignancy via targeting HPV.
Aceste test se face prin analiza unei probe human papillomavirus duration secretii, asemanator probei preluate pentru testarea Babes Papanicolau. Lucruri poti face pentru a se evita contactul cu virusul papiloma uman şi de prevenire a negilor: Things you can do to avoid contact with the human papillomavirus and prevent warts: Cu toate acestea, nu orice expunere la virusul papiloma uman rezultatele în curs de dezvoltare un neg, deoarece unele persoane sunt mai predispuse la a dezvolta infectie decat altii.
Full Text AI-92-08 PAPILLOMAVIRUS IN VITRO CELL CULTURE SYSTEMS NIH GUIDE, Volume 21, Number 36, October 9, 1992 RFA: AI-92-08 P.T. 34 Keywords: Viral Studies (Virology) Cell Lines Chemotherapeutic Agents National Institute of Allergy and Infectious Diseases Letter of Intent Receipt Date: November 6, 1992 Application Receipt Date: December 10, 1992 PURPOSE The Antiviral Research Branch of the Division of Microbiology and Infectious Diseases (DMID), National Institute of Allergy and Infectious Diseases (NIAID), invites Cooperative Agreement applications from organizational entities willing to participate, with the assistance of the NIAID, in furthering innovative in vitro approaches to the study of papillomavirus infections and their therapeutic control. The goals of this solicitation are to stimulate the use of in vitro papillomavirus replication systems for research on (1) the events of papillomavirus replication and pathogenesis and (2) the antiviral potential of experimental therapeutic ...
The high-risk types of human papillomaviruses (HPV), in particular PV 16 and 18 are responsible for the development of almost all cases of cervical cancer, for a substantial fraction of other malignant anogenital tumors (penis, vulva and perianum) and for a proportion of head and neck cancer. The natural history of HPV infections and immunization experiments in animals with their respective papillomaviruses (e.g. the canine oral papillomavirus) clearly revealed the involvement of the immune system in controlling the viral infections and the diseases associated therewith. Antibodies appear to be the key molecules in preventing of an infection whereas mostly T cells and cytokines are involved in controlling virus persistence and progression towards malignancy. During the natural course of infection human papillomaviruses are not particularly immunogenic since their biology makes them barely visible by the immune system (infection is confined to the epithelium) but also since it has acquired ...
Results The seroprevalence for any HPV type and any of the types HPV-6/11/16/18 was 64.8% and 34.4%, respectively. 30.3% of adults were seropositive for any mucosal high-risk (HR) HPV, and HPV-58 (10.6%), HPV-16 (9.7%) and HPV-18 (9.3%) were the three most common types. 24.8% of seropositive individuals were positive for multiple mucosal HR-HPV serotypes. Seroprevalence for most HPV types was similar among men and women. While mucosal low-risk HPV seropositivity was found to significantly decrease with age, the prevalence of antibodies to mucosal HR antigens showed a general trend of increase with age. The lifetime number of sex partners was independently associated with mucosal HR-HPV seropositivity. Positive correlation of spousal seropositivity was observed for mucosal HPV but not for cutaneous HPV.. ...
Older research outputs will score higher simply because theyve had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 133,501 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 55% of its contemporaries ...
Infection by HPV (the human papillomavirus) is common. WebMD explains the risks of infection and the strains of HPV that can lead to cancer.
The Report Human papillomavirus (HPV) Associated Cancer - Pipeline Review, H2 2015 provides information on pricing, market analysis, shares, forecast, and company profiles for key industry participants. - MarketResearchReports.biz
The Report Human papillomavirus (HPV) Associated Cancer Pipeline Review, H2 2012 provides information on pricing, market analysis, shares, forecast, and company profiles for key industry participants. - MarketResearchReports.biz
LONDON, Oct. 12, 2017 /PRNewswire/ -- Human Papillomavirus (HPV) Associated Cancer - Pipeline Review, H2 2017SummaryDownload the full report: https://www.re...
A prospective cohort study was done to evaluation p53 codon 72 polymorphism in predictive the outcome of cervical cancer with other factors (94). Among 39 patients with HPV-16 positive cervical cancer, there was no difference between the lymph nodal metastases and p53 codon 72 polymorphism (Arg/Arg 54.5% vs 67.9%, Pro/Pro 0 vs 7.1%, Arg/Pro 45.5% vs 21.4%). The p53 codon 72 genotype did not influence the disease-free survival significantly (94). Similarly, another two studies also showed that there is no significant difference between p53 polymorphism and prognosis of cervical cancer patients (96,98).. Discussion. The development of cervical cancer correlates with some risk factors (7-11). There is evidence showing that certain human papillomavirus types, such as HPV-16 and HPV-18 are the main cause of cervical cancer (3-5). HPV-16 and HPV-18 encode two major oncoproteins, E6 and E7. The E6 protein binds to the cellular tumour-suppressor protein p53 and directs its degradation through the ...
Human papillomavirus and human cytomegalovirus DNAs presence in patients suspected of condylomatosis or papillomatosis. Although vaccination may protect you against cervical cancerit is not a substitute for regular cervical screening.
Human papillomavirus type 16 (HPV16) and type 18 (HPV18) are the two most prevalent high-risk HPVs for causing cervical cancer-a cancer that claims the lives of more than 250,000 women around the world every year. They transform healthy keratinocytes - cells that line the cervix - into cancerous cells by disrupting the host cell cycle. In particular, they use viral genes E6 and E7 to encode proteins E6 and E7 that bind and inactivate tumor-suppressor proteins p53 and pRb, respectively.. As E6 and E7 proteins of different high-risk HPVs have similar functions, however, the reason for HPV18 having a higher potential for causing cervical cancer is still unknown. Francoise Thierry at the A*STAR Institute of Medical Biology and co-workers have now solved this mystery through a study of the transcriptional regulation of E6 and E7.. E2F is a family of transcription factors that regulate the transcription of S-phase and mitotic genes, which are essential for cell duplication and division. There are ...
As of June 2015, 19 pts have been enrolled. Safety data is available for 13 pts. Cohort 1: n = 3, Cohort 2: n = 10; 12 males; median age 57.7 years; cancers at base of tongue = 6, tonsil = 6, soft palate = 1; median follow-up is 104 days. INO-3112 was well tolerated with no Grade 3 or higher AEs. The most common AEs were injection site pain (n = 11), local erythema (n = 4) and hematoma/swelling (n = 2, each). 2 subjects had Grade 3 lymphopenia at baseline without worsening during the trial. There was a Grade 2, unrelated SAE of post-surgical procedure hemorrhage. Enrollment and correlative analysis are ongoing; among evaluable samples tested to date, as compared to baseline, 4 of 5 had elevated anti HPV16 and 18 E6/E7 antibody titers. 9 of 10 exhibited increase in HPV-specific IFN-gamma ELISpot. 7 of 8 had HPV-specific CD8+ T cell activation concurrent with increased lytic proteins (granzymes and perforin). All tested pts had positive cellular immune responses in at least one test. ...
My research group studies the molecular mechanisms that contribute to the oncogenicity of high-risk human papillomaviruses (HPVs), which cause almost all cases of cervical carcinomas and a large proportion of other anogenital tract and oral cancers. While cervical cancer rates have been decreasing in the US, rates of HPV associated anal and oral cancers are on the rise, and cervical carcinomas remain a major cause of cancer death in women, worldwide. Current prophylactic vaccines do not alter disease progression in already infected individuals and since vaccination rates in the US remain low, high-risk HPV infections will remain a major cause of morbidity and mortality. We also investigate cutaneous HPVs, as well as certain animal papillomaviruses, to investigate how infections with these viruses contribute to skin carcinogenesis.. ...
The HBE4-E6/E7 (formerly NBE4-E6/E7) cell line was derived from normal bronchial epithelium taken from a man undergoing a left upper lobectomy for a poorly differentiated (T2 N0) adenocarcinoma. Cells from the primary explant in their first passage were transfected with the p1321 plasmid encoding the E6 and E7 genes of Human Papilloma virus type 16 (HPV 16) under the control of the human beta actin promotor.
High-risk human papillomaviruses are causative agents of cervical cancer. Viral protein E7 is required to establish and maintain the pro-oncogenic phenotype in infected cells, but the molecular mechanisms by which E7 promotes carcinogenesis are only partially understood. Our transcriptome analyses in primary human fibroblasts transduced with the viral protein revealed that E7 activates a group of mitotic genes via the activator B-Myb-MuvB complex. We show that E7 interacts with the B-Myb, FoxM1 and LIN9 components of this activator complex, leading to cooperative transcriptional activation of mitotic genes in primary cells and E7 recruitment to the corresponding promoters. E7 interaction with LIN9 and FoxM1 depended on the LXCXE motif, which is also required for pocket protein interaction and degradation. Using E7 mutants for the degradation of pocket proteins but intact for the LXCXE motif, we demonstrate that E7 functional interaction with the B-Myb-MuvB complex and pocket protein degradation ...
Human papillomaviruses (HPVs) are small double‐stranded DNA viruses that are associated with greater than 95% of cervical cancers and 20% of head and neck cancers
Once inside the host cell, HPV DNA replicates as the basal cells differentiate and progress to the surface of the epithelium. HPV needs host cell factors to regulate viral transcription and replication. E6-induced degradation of these proteins potentially causes loss of cell-cell contacts mediated by tight junctions and thus contributes to the loss of cell polarity seen in HPV-associated cervical cancers
Blockade of IL-10 signalling clears chronic viral and bacterial infections. Immunization together with blockade of IL-10 signalling or relatively low level of IL-10 further enhances viral and bacterial clearance. IL-10 functions through binding to interleukin 10 receptor (IL- 10R). Here we showed that peptides P1 and P2 with the hydrophobic and hydrophilic pattern of the IL10R-binding helix in IL-10 could bind with either IL-10R1 or IL-10, and inhibit inflammatory signals with long duration and negligible cytotoxicity in vitro. Furthermore, P2 can enhance antigen specific CD8+ T cell responses in mice induced by the vaccine based on a long peptide of protein E7 in a human papillomavirus type 16; ;
Bakgrunn: Infeksjon med humant papillomavirus er den vanligste seksuelt overførbare infeksjonen både hos kvinner og menn. Det er anslått at opptil 20 % av den seksuelt aktive befolkningen på et hvert tidspunkt er smittet, og at over 70 % av befolkningen vil få en HPV- infeksjon i løpet av sitt seksuelt aktive liv. Høsten 2009 ble vaksinen mot humant papilloma virus (HPV) innført i barnevaksinasjonsprogrammet for jenter på 7. klassetrinn. Som for alle vaksiner i barnevaksinasjonsprogrammet er dette et tilbud man kan velge å ta i mot. Siden denne vaksinen er ny, vil det være usikkerhet knyttet til i hvor stor grad foreldrene vil tillate at vaksinen anvendes på døtrene. I denne forbindelse har jeg valgt å undersøke vurderinger foreldrene gjør for å bestemme seg om de skal la døtrene sine vaksineres eller ikke. Material og metode: Denne oppgaven er et litteraturstudium. I utarbeidelsen har jeg brukt søkeportalen PubMed. I samarbeid med veileder har jeg funnet de mest relevante ...
Low MicroRNA-21 Expression in HPV-Induced Carcinogenesis ➨ Low microRNA-21 expression in the development of a favorable microenvironment for HPV-induced carcinogenesis Introduction Human papillomaviruses (HPVs)
Regeneron Pharmaceuticals, Inc. and ISA Pharmaceuticals B.V., a clinical-stage immunotherapy company, today announced a clinical collaboration to advance ISA101, an immunotherapy targeting human papillomavirus type 16 (HPV16)-induced cancer, in...
Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
SWISS-MODEL Template Library (SMTL) entry for 1dto.1. CRYSTAL STRUCTURE OF THE COMPLETE TRANSACTIVATION DOMAIN OF E2 PROTEIN FROM THE HUMAN PAPILLOMAVIRUS TYPE 16
余白の削除などで一部分だけ印刷したい場合、または画像が薄すぎる、暗すぎる場合は、下の「詳細設定」をお試しください ...
Infecţia cu virusul papiloma uman HPV este principala cauză. All these efforts in terms of public funds and the cured persons mobilization did not succeed to change the incidence and the mortality by cervical cancer.
O astfel de ITS care a fost neglijată, poate duce la complicaţii pe termen lung. La meilleure protection contre les IST est d utiliser un préservatif masculin ou féminin.
Apr 1 ; 7 E6 binds to p53 via a cellular ubiquitin ligase named E6AP, so that it becomes ubiquitinated, leading to degradation and down-regulation of pathways involved in cycle arrest and apoptosis.
The epigenetic regulation in CaSki cells transfected with E6AP siRNA was investigated. DNMTs (Dnmt3a and Dnmt3b) and MBDs expression levels were analyzed. - A. Botezatu, D Socolov, CD Goia, IV Iancu, C Ungureanu, I Huica, G Anton, The relationship between HPV16 and HPV18 viral load and cervical lesions progression, Roumanian Arch Microbiol Immunol, 2009, 3, 175-182, ISSN 0004-0037 PubMed ...
Ectopiile pot fi congenitale urmare a unei tulburări de dezvoltare embriologică sau dobândite prin slăbirea mijloacelor de fixare, traumatisme sau tumori ce acţionează prin presiune asupra rinichilor. Este mai friabil decât ficatul sau splina. Stadiu extensiv - cancerul s-a imprastiat cancer plamani doare si catre cel de-al doilea plaman, catre pleura sau catre alte organe din organism.
... and human papillomavirus E7 protein share the capacity to disrupt the interaction between transcription factor E2F and the ... "Systematic identification of interactions between host cell proteins and E7 oncoproteins from diverse human papillomaviruses". ... 1986 Dyson, N.; Howley, P.; Munger, K.; Harlow, E. (1989). "The human papilloma virus-16 E7 oncoprotein is able to bind to the ... 1989 Werness, B.; Levine, A.; Howley, P. (1990). "Association of human papillomavirus types 16 and 18 E6 proteins with p53". ...
He first studied a bovine papillomavirus, then focused on the E5, E6, and E7 proteins of the human papillomavirus. In 1988, ...
pRb is one of the targets of the oncogenic human papilloma virus protein E7, and human adenovirus protein E1A. By binding to ... Most E2F have a pocket protein binding domain. Pocket proteins such as pRB and related proteins p107 and p130, can bind to E2F ... Homo sapiens E2F1 mRNA or E2F1 protein sequences from NCBI protein and nucleotide database. X-ray crystallographic analysis has ... Activator E2F proteins can then transcribe S phase promoting genes. In REF52 cells, overexpression of activator E2F1 is able to ...
SV40 large TAg, other polyomavirus large T antigens, adenovirus E1a proteins, and oncogenic human papillomavirus E7 proteins ... Protein-protein interactions between T-antigen and DNA polymerase-alpha directly stimulate replication of the virus genome. T- ... "The binding domain structure of retinoblastoma-binding proteins". Protein Science. 2 (2): 155-64. doi:10.1002/pro.5560020204. ... The SV40 large T-antigen has been used as a model protein to study nuclear localization signals (NLSs). It is imported into the ...
TSAs can be products of oncoviruses like E6 and E7 proteins of Human papillomavirus, occurring in cervical carcinoma, or EBNA-1 ... "Human papillomavirus type 16 E6/E7-specific cytotoxic T lymphocytes for adoptive immunotherapy of HPV-associated malignancies ... Another example of TSAs are abnormal products of mutated oncogenes (e.g. Ras protein) and anti-oncogenes (e.g. p53). Tumor- ... Weon JL, Potts PR (December 2015). "The MAGE protein family and cancer". Current Opinion in Cell Biology. 37: 1-8. doi:10.1016/ ...
2005). "Association of the human papillomavirus type 16 E7 oncoprotein with the 600-kDa retinoblastoma protein-associated ... 2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi: ... E3 ubiquitin-protein ligase UBR4 is an enzyme that in humans is encoded by the UBR4 gene. GRCh38: Ensembl release 89: ... The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 7 (1): 65-73. doi: ...
... papillomavirus e7 proteins MeSH D12.776.624.664.520.750 - retroviridae proteins, oncogenic MeSH D12.776.624.664.520.750.320 - ... groel protein MeSH D12.776.602.500.500.100 - fusion proteins, bcr-abl MeSH D12.776.602.500.500.320 - fusion proteins, gag-onc ... oncogene protein v-maf MeSH D12.776.964.700.750.875 - oncogene proteins v-abl MeSH D12.776.964.700.750.882 - oncogene proteins ... fusion proteins, gag-pol MeSH D12.776.964.775.350.400 - hiv core protein p24 MeSH D12.776.964.775.375.325 - fusion proteins, ...
In most papillomavirus types, the primary function of the E7 protein is to inactivate members of the pRb family of tumor ... Only a few papillomavirus types encode a short protein from the E8 gene. In the case of BPV-4 (papillomavirus genus Xi), the E8 ... There are cancer causing papillomavirus genome that encodes two small proteins called E6 and E7 that mimic cancer causing ... The E5 protein of some animal papillomavirus types (mainly bovine papillomavirus type 1) functions as an oncogene primarily by ...
Also, miR-203 has been identified as target of human papillomavirus (HPV) protein E7, which causes its downregulation and thus ... "Human papillomaviruses modulate expression of microRNA 203 upon epithelial differentiation to control levels of p63 proteins". ... In lung cancer cell lines, miR-203 has been shown to target DKK1, a secreted protein which acts as a survival factor in certain ... Sonkoly E, Wei T, Pavez Loriè E, Suzuki H, Kato M, Törmä H, Ståhle M, Pivarcsi A (2010). "Protein kinase C-dependent ...
HPV E6 and E7 are inhibitory proteins expressed by the human papillomavirus, HPV being responsible for the formation of the ... Examples of viral Bcl-2 proteins include the Epstein-Barr virus BHRF1 protein and the adenovirus E1B 19K protein.[95] Some ... The adenovirus E1B-55K protein and the hepatitis B virus HBx protein are examples of viral proteins that can perform such a ... these inhibitory proteins target retinoblastoma tumor-suppressing proteins.[74] These tumor-suppressing proteins regulate the ...
The early region encodes nonstructural proteins E1 to E7. There are three viral oncoproteins, E5, E6 and E7; BPVs of the ... Similar papillomaviruses of ungulates (e.g. deer papillomavirus, European elk papillomavirus, ovine papillomavirus 1,2) are ... Campo, MS (2006). "Bovine papillomavirus: old system, new lessons?". In Campo, MS (ed.). Papillomavirus Research: From Natural ... L1 protein or (for BPV-4) L2 protein confers long-lasting protection against challenge with the same BPV type, but is generally ...
Transforming proteins E6 and E7 induce the S-phase in the lower epithelial layers. Viral replication proteins E1 and E2 are ... The E2 protein overlaps with the E4 open reading frames in the other papillomaviruses. These differences in the E2 proteins ... L2 proteins gather at PML body nuclear structures and recruit L1 proteins during virus assemblage. L2 proteins are not ... Expression of the papillomavirus E4 protein correlates with the onset of viral DNA amplification. Using a mutant cottontail ...
The E6/E7 proteins inactivate two tumor suppressor proteins, p53 (inactivated by E6) and pRb (inactivated by E7).[14] The viral ... E7 (in oncogenic HPVs) acts as the primary transforming protein. E7 competes for retinoblastoma protein (pRb) binding, freeing ... E6/E7 proteins[edit]. The two primary oncoproteins of high risk HPV types are E6 and E7. The "E" designation indicates that ... serves as an E7 mRNA to translate E7 protein.[99] However, viral early transcription subjects to viral E2 regulation and high ...
A multifunctional protein involved in the transcription and replication of viral RNAs. Protein E7 (Human Papillomavirus): Plays ... UniProt entry on protein E7. Retrieved 27 July 2020. NCBI entry on MIPOL1 orthologs. Retrieved 30 June 2020 NCBI Conserved ... MIPOL1 protein is evolving at a moderate rate relative to fast evolving protein such as fibrinogen alpha, and slow evolving ... This protein is known to interact with multiple human proteins, verified via two-hybrid screening. A few notable examples ...
In Papillomaviruses, they are called E1 through E7 and also stimulate cellular replication. In Polyomavirus, the early proteins ... The late proteins make up the virus capsid. In Polyomaviruses, they are known as VP1, VP2, and VP3; in Papillomaviruses they ... The early proteins produced in Papillomaviruses and Polyomaviruses regulate the cell cycle and activate DNA replication. ... The late class consists primarily of structural proteins and assembly enzymes, and is dependent in all three families on the ...
... liquid crystal mixture E7, the Lie group in mathematics E7 polytope, in geometry E7 papillomavirus protein E07, an ICD-10 ... route E7 in Japan Cheras-Kajang Expressway, route E7 in Malaysia Boeing E-7, either: Boeing E-7 ARIA, the original designation ... an international road Peugeot E7, a hackney cab PRR E7, a steam locomotive Carbon Motors E7,a police car E7 series, a ... Economy 7, an electricity tariff E-7 enlisted rank in the military of the United States E7 (countries) E7, a musical note in ...
The pRb protein is inactivated by E7 in HPV+OPC, but in HPV-OPC it is the p16 tumour suppressor part of the pRb tumour ... Human papillomavirus-positive oropharyngeal cancer (HPV-positive OPC or HPV+OPC), is a cancer (squamous cell carcinoma) of the ... Its genome encodes the early (E) oncoproteins E5, E6 and E7 and the late (L) capsid proteins L1 and L2. The virus gains access ... Also E6 and E7 may make HPV+OPC more immunogenic than HPV-OPC, since anti-E6 and E7 antibodies may be detected in these ...
Dyson demonstrated that the retinoblastoma protein can form complexes in vitro with the E7 oncoprotein of Human papilloma virus ... Dyson, N; Howley, PM; Munger, K; Harlow, E (1989). "The human papilloma virus-16 E7 oncoprotein is able to bind to the ... This result implicated pRB binding to E7 as a step in human papilloma virus-associated carcinogenesis. More recently, Dyson's ... The Dyson Lab studies the retinoblastoma protein. Working as a post-doctoral fellow in the laboratory of Dr. Ed Harlow, ...
... a homolog of the Drosophila tumor suppressor protein Tid56, can interact with the human papillomavirus type 16 E7 oncoprotein ... This protein belongs to the DNAJ/Hsp40 protein family, which is known for binding and activating Hsp70 chaperone proteins to ... where it can also interact with cytosolic proteins and possibly function to transport these proteins. This protein is ... tid gene defines a novel Ras GTPase-activating protein (RasGAP)-binding protein". J. Biol. Chem. 276 (16): 13087-95. doi: ...
... of E7 protein and thus demonstrated potential use of these nanosensors in detection and diagnosis of human papillomavirus, ... The measurement can be achieved by using the following methods: binding active nanoparticles to active proteins within the cell ... using site-directed mutagenesis to produce indicator proteins, allowing for real-time measurements, or by creating a ... "Controllable Activation of Nanoscale Dynamics in a Disordered Protein Alters Binding Kinetics". Journal of Molecular Biology. ...
... encoded transcripts in cervical cancer cells and confirmed the oncogenic role of the viral E6 and E7 proteins in HPV associated ... Systematic review of genomic integration sites of human papillomavirus genomes in epithelial dysplasia and invasive cancer of ... In 1997, Knebel Doeberitz discovered that a cellular protein referred to as p16INK4a, is uniformly overexpressed in HPV- ... Since the late 1980s, Knebel Doeberitz has investigated the role of human papilloma virus (HPV) ...
One such example, human papillomavirus (HPV), encodes a protein, E6, which binds to the p53 protein and inactivates it. This ... in synergy with the inactivation of the cell cycle regulator pRb by the HPV protein E7, allows for repeated cell division ... Mutant p53 proteins often fail to induce MDM2, causing p53 to accumulate at very high levels. Moreover, the mutant p53 protein ... In addition to the full-length protein, the human TP53 gene encodes at least 15 protein isoforms, ranging in size from 3.5 to ...
"The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product." Science 243.4893 (1989): 934- ... Among Harlow's discoveries was the demonstration that the retinoblastoma protein interacts with viral transforming proteins, ... Dyson, N (1989). "The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product". Science. 243 ... "The retinoblastoma protein is phosphorylated during specific phases of the cell cycle." Cell 58.6 (1989): 1097-1105. van den ...
... the E7 protein of Human papillomavirus (HPV), and the B18R protein of vaccinia virus.[23][24] Reducing IFN-α activity may ... and phosphorylates a translational repressor protein called eukaryotic translation-initiation factor 4E-binding protein 1 ( ... to prevent the activity of RNA-dependent protein kinases; this is the mechanism reovirus adopts using its sigma 3 (σ3) protein ... the phosphorylated eIF-2 forms an inactive complex with another protein, called eIF2B, to reduce protein synthesis within the ...
SV40 uses the same protein for inactivating Rb, LT, to inactivate p53.[39] HPV contains a protein, E7, which can bind to Rb in ... Human papilloma virus (HPV), a DNA virus, causes transformation in cells through interfering with tumor suppressor proteins ... DNA oncoviruses typically impair two families of tumor suppressor proteins: tumor proteins p53 and the retinoblastoma proteins ... the HPV protein E6 binds to a cellular protein called the E6-associated protein (E6-AP, also known as UBE3A), forming a complex ...
HPV can induce tumor by several mechanisms: E6 and E7 oncogenic proteins. Disruption of tumor suppressor genes. High-level DNA ... Hobbs, C.G.L.; Sterne, J.A.C.; Bailey, M.; Heyderman, R.S.; Birchall, M.A.; Thomas, S.J. (August 2006). "Human papillomavirus ... Some head and neck cancers are caused by Human papillomavirus (HPV). In particular HPV16, is a causal factor for some head and ... E6 sequesters p53 to promote p53 degradation while pRb inhibits E7. p53 prevents cell growth when DNA is damaged by activating ...
... and the human papillomavirus oncoprotein-58 E7 are naturally occurring lysine-less proteins that are degraded by the ubiquitin ... coordinating the cellular localization of proteins, activating and inactivating proteins, and modulating protein-protein ... Reinstein E, Scheffner M, Oren M, Ciechanover A, Schwartz A (November 2000). "Degradation of the E7 human papillomavirus ... The protein modifications can be either a single ubiquitin protein (monoubiquitylation) or a chain of ubiquitin ( ...
... is a patented construct consisting of the HPV Type 16 E7 protein and heat shock protein 65 (Hsp65) and is currently the ... 1999). "Human papillomavirus is a necessary cause of invasive cervical cancer worldwide". J. Pathol. 189 (1): 12-9. doi:10.1002 ... Some of the HPV "early" genes, such as E6 and E7, are known to act as oncogenes that promote tumor growth and malignant ... 2007). "Case-control study of human papillomavirus and oropharyngeal cancer". N. Engl. J. Med. 356 (19): 1944-56. doi:10.1056/ ...
2007). "Human papillomavirus-16 E7 interacts with Siva-1 and modulates apoptosis in HaCaT human immortalized keratinocytes". J ... Apoptosis regulatory protein Siva is a protein that in humans is encoded by the SIVA1 gene. This gene encodes a protein with an ... 2006). "The zinc containing pro-apoptotic protein siva interacts with the peroxisomal membrane protein pmp22". Mol. Cell. ... 2000). "Apoptosis in coxsackievirus B3-caused diseases: interaction between the capsid protein VP2 and the proapoptotic protein ...
E7 (in oncogenic HPVs) acts as the primary transforming protein. E7 competes for retinoblastoma protein (pRb) binding, freeing ... Role in cancer The E6/E7 proteins inactivate two tumor suppressor proteins, p53 (inactivated by E6) and pRb (inactivated by E7 ... One of the spliced isoform RNAs, E6*I, serves as an E7 mRNA to translate E7 protein. However, viral early transcription ... E6 produces a protein (also called E6) that binds to and inactivates a protein in the host cell called p53. Normally, p53 acts ...
doi:10.1097/01.ju.0000158155.33890.e7. PMC 2948569 . PMID 15879786.. *^ Cooperberg MR, Freedland SJ, Pasta DJ, Elkin EP, Presti ... The protein ZIP1 is responsible for the active transport of zinc into prostate cells. One of the zinc's important roles is to ... Papilloma virus has been proposed in several studies to have a potential role in prostate cancer, but as of 2015 the evidence ... Prostate specific membrane antigen is a transmembrane carboxypeptidase and exhibits folate hydrolase activity.[75] This protein ...
HPV E6 and E7 are inhibitory proteins expressed by the human papillomavirus, HPV being responsible for the formation of the ... Examples of viral Bcl-2 proteins include the Epstein-Barr virus BHRF1 protein and the adenovirus E1B 19K protein.[104] Some ... The adenovirus E1B-55K protein and the hepatitis B virus HBx protein are examples of viral proteins that can perform such a ... these inhibitory proteins target retinoblastoma tumor-suppressing proteins.[83] These tumor-suppressing proteins regulate the ...
Should an oncogenic protein, such as those produced by cells infected by high-risk types of human papillomaviruses, bind and ... "Inhibition of CDK activity and PCNA-dependent DNA replication by p21 is blocked by interaction with the HPV-16 E7 oncoprotein" ... The retinoblastoma protein (protein name abbreviated pRb; gene name abbreviated RB or RB1) is a tumor suppressor protein that ... protein binding. • androgen receptor binding. • identical protein binding. • enzyme binding. • ubiquitin protein ligase binding ...
Kanduc D (Jul 2002). "Translational regulation of human papillomavirus type 16 E7 mRNA by the peptide SEQIKA, shared by rabbit ... Suzuki H, Fukunishi Y, Kagawa I, Saito R, Oda H, Endo T, Kondo S, Bono H, Okazaki Y, Hayashizaki Y (Oct 2001). "Protein-protein ... "A proteomics strategy to elucidate functional protein-protein interactions applied to EGF signaling". Nature Biotechnology. 21 ... Keratin, type II cytoskeletal 7 also known as cytokeratin-7 (CK-7) or keratin-7 (K7) or sarcolectin (SCL) is a protein that in ...
doi:10.1097/01.ju.0000158155.33890.e7. PMC 2948569. PMID 15879786.. *^ Cooperberg MR, Freedland SJ, Pasta DJ, Elkin EP, Presti ... Transportni protein ZIP1 odgovoran je za transport cinka u ćelije prostate. Jedna od važnih uloga cinka je promjena ćelijskog ... Heidegger I, Borena W, Pichler R (maj 2015). "The role of human papilloma virus in urological malignancies". Anticancer ... Cai T, Di Vico T, Durante J, Tognarelli A, Bartoletti R (decembar 2018). "Human papilloma virus and genitourinary cancers: a ...
"for their discovery of G-proteins and the role of these proteins in signal transduction in cells"[91] ... "for his discovery of human papilloma viruses causing cervical cancer"[105]. 弗朗索瓦丝·巴尔-西诺西 法国 "发现人类免疫缺陷病毒(即艾滋病病毒)". "for their ... "for the discovery that proteins have intrinsic signals that govern their transport and localization in the cell"[96] ... "in recognition of the contributions to our knowledge of cell chemistry made through his work
... a therapeutic synthetic DNA vaccine targeting human papillomavirus 16 and 18 E6 and E7 proteins for cervical intraepithelial ... Human papilloma virus (HPV) vaccines are vaccines that prevent infection by certain types of human papillomavirus.[1] Available ... Its protein makeup allows it to target four types of HPV. Gardasil contains inactive L1 proteins from four different HPV ... In general, these vaccines focus on the main HPV oncogenes, E6 and E7. Since expression of E6 and E7 is required for promoting ...
Papilloma virus has been proposed to have a potential role, but as of 2015, the evidence was inconclusive; as of 2018, the ... Transport protein ZIP1 is responsible for the transport of zinc into prostate cells. One of zinc's important roles is to change ... doi:10.1097/01.ju.0000158155.33890.e7. PMC 2948569. PMID 15879786. Cooperberg MR, Freedland SJ, Pasta DJ, Elkin EP, Presti JC, ... Cai T, Di Vico T, Durante J, Tognarelli A, Bartoletti R (December 2018). "Human papilloma virus and genitourinary cancers: a ...
Hawley-Nelson, P; Vousden, KH; Hubbert, NL; Lowy, DR; Schiller, JT (1 December 1989). "HPV16 E6 and E7 proteins cooperate to ... For his joint research with John T. Schiller on technology that enabled the development of preventive human papillomavirus (HPV ... Lowy's HPV research, conducted in collaboration with John T. Schiller, has characterized the HPV oncoproteins E6 and E7. Nathan ... Androphy, EJ; Hubbert, NL; Schiller, JT; Lowy, DR (April 1987). "Identification of the HPV-16 E6 protein from transformed mouse ...
"Human Papillomavirus (HPV) and Cancer". CDC. January 2, 2018. Retrieved March 22, 2018. Münger K, Baldwin A, Edwards KM, ... Among high-risk HPV viruses, the HPV E6 and E7 oncoproteins inactivate tumor suppressor genes when infecting cells. In addition ... Insulin-like growth factors and their binding proteins play a key role in cancer cell growth, differentiation and apoptosis, ... These include human papillomavirus (cervical carcinoma), Epstein-Barr virus (B-cell lymphoproliferative disease and ...
... product candidates directed against human papillomavirus (HPV)-16 E6 and E7 oncoproteins for the treatment of HPV-associated ... AIS will grant Kite an exclusive license to two programs from its transmembrane immunomodulatory protein (TIP) technology, ... a protein expressed on the cell surface of B-cell lymphomas and leukemias. In the same month, Kite Pharma and GE Global ... a protein expressed on the cell surface of B-cell lymphomas and leukemias. It garnered a US FDA Breakthrough Therapy ...
For medium protein overexpression/gene amplification concordance varies, but is higher when monoclonal antibodies are used than ... Apart from cancers, CISH has also been shown to be useful in detecting human papillomavirus infections. SISH uses a similar ... E6/E7 mRNA test, and potential clinical applications". BioMed Research International. 2013: 1-11. doi:10.1155/2013/453606. PMC ... On the next day, the sample is washed and a blocker for nonspecific protein binding sites is applied. If horseradish peroxidase ...
2013). "A Chlamydomonas-Derived Human Papillomavirus 16 E7 Vaccine Induces Specific Tumor Protection". PLOS ONE. 8 (4): e61473 ... a human antibody protein, human Vascular endothelial growth factor, a potential therapeutic Human Papillomavirus 16 vaccine, a ... These proteins and others like them are increasingly widely used in the field of optogenetics. The genome of C. Reinhardtii is ... Channelrhodopsin-1 and Channelrhodopsin-2, proteins that function as light-gated cation channels, were originally isolated from ...
Major, E. O. (1983). "JC virus T protein expression in owl monkey tumor cell lines". Progress in Clinical and Biological ... The papovaviruses have since been split into two categories: papillomaviruses and polyomaviruses.) In his first faculty ... "Human articular chondrocytes immortalized by HPV-16 E6 and E7 genes: Maintenance of differentiated phenotype under defined ... "A variant of adenovirus 12 producing cytoplasmic accumulation of capsid proteins". The Journal of General Virology. 40 (3): 685 ...
E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from ... Human papillomavirus type 92. Macaca fascicularis papillomavirus type 1. And more. 94. UniRef50_Q80901. Cluster: Protein E7. 51 ... Human papillomavirus type 15. Human papillomavirus. 93. UniRef90_P36820. Cluster: Protein E7. 2. ... sp,P36820,VE7_HPV15 Protein E7 OS=Human papillomavirus type 15 GN=E7 PE=3 SV=1 ...
E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from ... sp,Q705D1,VE7_HPV94 Protein E7 OS=Human papillomavirus type 94 OX=260717 GN=E7 PE=3 SV=1 ... to allow unambiguous identification of a protein.,p>,a href=/help/protein_names target=_top>More...,/a>,/p>Protein namesi. ... E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from ...
... epidemiological and molecular findings link some types of Human Papillomaviruses (HPV) with cancer of the genital tract. They ... Human papillomavirus E6 and E7: proteins which deregulate the cell cycle Bioessays. 1995 Jun;17(6):509-18. doi: 10.1002/bies. ... Extensive studies on HPV E6 and E7 proteins have demonstrated their involvement in malignant transformation. E6 and E7 bind the ... The E6/p53 and E7/Rb1 interactions result in a deregulation of the cell cycle with loss of control of crucial cellular events, ...
E7 Protein - Pipeline Review, H1 2016 report by Global Markets Direct. Global Markets Directs, Human Papilloma Virus (HPV) E7 ... Human Papilloma Virus (HPV) E7 Protein - Products under Development by Therapy Area 11 Human Papilloma Virus (HPV) E7 Protein ... Human Papilloma Virus (HPV) E7 Protein - Products under Development by Companies 15 Human Papilloma Virus (HPV) E7 Protein - ... Human Papilloma Virus (HPV) E7 Protein - Dormant Projects 88 Human Papilloma Virus (HPV) E7 Protein - Discontinued Products 90 ...
Production of Human Papillomavirus Type 16 E7 Protein in Lactococcus lactis. L. G. Bermúdez-Humarán, P. Langella, A. Miyoshi, A ... Production of Human Papillomavirus Type 16 E7 Protein in Lactococcus lactis. L. G. Bermúdez-Humarán, P. Langella, A. Miyoshi, A ... Production of Human Papillomavirus Type 16 E7 Protein in Lactococcus lactis. L. G. Bermúdez-Humarán, P. Langella, A. Miyoshi, A ... Production of Human Papillomavirus Type 16 E7 Protein in Lactococcus lactis Message Subject (Your Name) has forwarded a page to ...
As purified E7 protein is not available, we used the Nuc-E7 protein concentration as determined by anti-Nuc antibodies as the ... Interestingly, although pCYT-Nuc-E7 and pSEC-Nuc-E7 have an essentially identical design, the yield of exported Nuc-E7 protein ... 1) (40). In pCYT-E7, the fragment encoding SPUsp45 is absent. In both pSEC-E7 and pCYT-E7, expression of the E7 cassettes is ... B) Estimation of Nuc-E7 (pSEC-Nuc-E7) and native E7 (pSEC-E7) yields in L. lactis. Production yields were estimated for the ...
6b E7), HPV-16 E7 mutant that fails to bind pRb (16E7mut p24gly; 16 E7 mut), HPV-16 E7 (16 E7), transcription factor E2F-1, HPV ... HPV-16 E7 Protein Down-Regulates TGF-β2 Production by a pRb-dependent Pathway.. The HPV-16 E7 protein binds to and inactivates ... Specifically, the low-risk HPV-6b E7 protein and an HPV-16 E7 mutant [E7-p24gly (33)] , which do not bind pRb, also do not ... A mutant HPV-16 E7 protein unable to bind the retinoblastoma protein [E7-p24gly (33)] did not diminish secretion of ...
Enhanced Cytotoxic CD8 T Cell Priming Using Dendritic Cell-Expressing Human Papillomavirus-16 E6/E7-p16INK4 Fusion Protein with ... We developed potential adenoviral vaccines that express a fusion protein of HPV-16 E6 and E7 (Ad.E6E7) alone or fused with p16 ... HPV-related cancers express the viral oncoproteins E6 and E7. The latter inactivates the tumor suppressor protein ... The incidence of human papillomavirus (HPV)-related head and neck squamous cell carcinoma has increased in recent decades, ...
The human papillomavirus type 16 E7 protein belongs to a family of nuclear oncoproteins that share amino acid sequences and ... Chemical synthesis of human papillomavirus type 16 E7 oncoprotein: autonomous protein domains for induction of cellular DNA ... Chemical synthesis of human papillomavirus type 16 E7 oncoprotein: autonomous protein domains for induction of cellular DNA ... Chemical synthesis of human papillomavirus type 16 E7 oncoprotein: autonomous protein domains for induction of cellular DNA ...
1A). These data show that E7 protein was expressed in LL-E7 (lane 1) but not in LL-RP-GD (lane 2). The E7 protein was detected ... Expression of E7 and IL-12 in vitro. To confirm E7 protein expression in the L. Lactis strains, E7 protein levels were detected ... A) Production of human papillomavirus-16 E7 by recombinant L. lactis was analyzed using anti-E7 antibodies. E7 antigen was ... For construction of LL-E7P-IL-12D, the full protein-coding region of the HPV-16 E7 gene was amplified by polymerase chain ...
Recombinant Human Papillomavirus Type 16 E7 Protein as a Model Antigen to Study the Vaccine Potential in Control and E7 ... Recombinant Human Papillomavirus Type 16 E7 Protein as a Model Antigen to Study the Vaccine Potential in Control and E7 ... Recombinant Human Papillomavirus Type 16 E7 Protein as a Model Antigen to Study the Vaccine Potential in Control and E7 ... Recombinant Human Papillomavirus Type 16 E7 Protein as a Model Antigen to Study the Vaccine Potential in Control and E7 ...
E7)- Pipeline Review, H2 2019, provides in depth analysis on Human Papillomavirus ... E7) - Dormant Products. Human Papillomavirus Protein E7 (E7) - Discontinued Products. Human Papillomavirus Protein E7 (E7) - ... Human Papillomavirus Protein E7 (E7) - Overview. Human Papillomavirus Protein E7 (E7) - Therapeutics Development. Products ... Human Papillomavirus Protein E7 (E7) - Pipeline Review, H2 2019. Human Papillomavirus Protein E7 (E7) - Pipeline Review, H2 ...
Antibodies against the human papillomavirus type 16 early proteins in human sera: correlation of anti-E7 reactivity with ... The E7 protein of human papillomavirus (HPV) type 16 expressed by recombinant vaccinia virus can be used for detection of ... Antibodies against human papillomavirus (HPV) type 16 and 18 E2, E6 and E7 proteins in Sera: Correlation with presence of ... Prevalence of serum IgG antibodies for the E7 and L2 proteins of human papillomavirus type 16 in cervical cancer patients and ...
Expression of Human Papilloma Virus E7 Protein Causes Apoptosis and Inhibits DNA Synthesis in Primary Hepatocytes via Increased ... Conditionally Activated E7 Proteins of High-Risk and Low-Risk Human Papillomaviruses Induce S Phase in Postmitotic, ... Casein Kinase II Phosphorylation of the Human Papillomavirus-18 E7 Protein Is Critical for Promoting S-Phase Entry. Cell Growth ... Concordant Induction of Cyclin E and p21cip1 in Differentiated Keratinocytes by the Human Papillomavirus E7 Protein Inhibits ...
Phase I/II trial of immunogenicity of a human papillomavirus (HPV) type 16 E7 protein-based vaccine in women with oncogenic HPV ... Reference : Phase I/II trial of immunogenicity of a human papillomavirus (HPV) type 16 E7 protein.... ... In the present study, we tested the immunogenicity of a fusion protein (PD-E7) comprising a mutated HPV-16 E7 linked to the ... influenzae protein D (PD)-directed CD4(+) response. All the vaccinated patients, but not the placebo, made significant E7- and ...
The E7 proteins of low- and high-risk human papillomaviruses share the ability to target the pRB family member p130 for ... Human Papillomavirus Type 16 E7 Binds to E2F1 and Activates E2F1-driven Transcription in a Retinoblastoma Protein-independent ... Nonconserved Lysine Residues Attenuate the Biological Function of the Low-Risk Human Papillomavirus E7 Protein ... Interactions of the cellular CCAAT displacement protein and human papillomavirus E2 protein with the viral origin of ...
Hybridoma cell line producing CamVir-4 antibodies raised against E7 protein of Human Papilloma Virus 16 which are mouse ... Enquiry for E7 protein of human papillomavirus 16 monoclonal antibody CamVir-4. Reagents Enquiry. To find out more about how ... E7 protein of human papillomavirus 16 monoclonal antibody CamVir-4. Code: AB57 Hybridoma cell line producing CamVir-4 ... antibodies raised against E7 protein of human papillomavirus 16 which are mouse monoclonal antibodies. Has been used in western ...
... and the human papillomavirus E7 protein share a short amino acid sequence that constitutes a domain required for the ... We now show that the E7 protein and the simian virus 40 large tumor antigen can dissociate the E2F-pRb complex, dependent on ... and E7 to dissociate the E2F-pRb complex may be a common activity of these viral proteins that has evolved to stimulate ... also find that the E2F-pRb complex is absent in various human cervical carcinoma cell lines that either express the E7 protein ...
Transforming Properties of Beta-3 Human Papillomavirus E6 and E7 Proteins. Lucia Minoni, Maria Carmen Romero-Medina, Assunta ... Transforming Properties of Beta-3 Human Papillomavirus E6 and E7 Proteins. Lucia Minoni, Maria Carmen Romero-Medina, Assunta ... Transforming Properties of Beta-3 Human Papillomavirus E6 and E7 Proteins. Lucia Minoni, Maria Carmen Romero-Medina, Assunta ... Transforming Properties of Beta-3 Human Papillomavirus E6 and E7 Proteins Message Subject (Your Name) has forwarded a page to ...
Sera were examined for the presence of antibody against E7 protein of human papillomavirus type 16 (HPV‐16) by Western blot ... N2 - Sera were examined for the presence of antibody against E7 protein of human papillomavirus type 16 (HPV‐16) by Western ... AB - Sera were examined for the presence of antibody against E7 protein of human papillomavirus type 16 (HPV‐16) by Western ... Serum Antibody against Unfused Recombinant E7 Protein of Human Papillomavirus Type 16 in Cervical Cancer Patients. In: Japanese ...
In particular, E7 can bypass G0/G1 arrest in response to both serum withdrawal and loss of cell adhesion, two experimental ... Expression of the E7 oncogene of HPV-16 induces S phase entry of mammalian cells in the presence of antiproliferative signals. ... Papillomavirus E7 Proteins * Recombinant Fusion Proteins * Tumor Suppressor Proteins * oncogene protein E7, Human ... Inactivation of the CDK Inhibitor p27KIP1 by the Human Papillomavirus Type 16 E7 Oncoprotein Oncogene. 1996 Dec 5;13(11):2323- ...
Phase I/II trial of immunogenicity of a human papillomavirus (HPV) type 16 E7 protein-based vaccine in women with oncogenic HPV ... A recombinant vaccinia virus encoding human papillomavirus types 16 and 18, E6 and E7 proteins as immunotherapy for cervical ... A recombinant vaccinia virus encoding human papillomavirus types 16 and 18, E6 and E7 proteins as immunotherapy for cervical ... Two HPV proteins, E6 and E7, are consistently expressed in tumour cells. The objectives of the study were to examine the ...
A recombinant vaccinia virus encoding human papillomavirus types 16 and 18, E6 and E7 proteins as immunotherapy for cervical ... A recombinant vaccinia virus encoding human papillomavirus types 16 and 18, E6 and E7 proteins as immunotherapy for cervical ... Two HPV proteins, E6 and E7, are consistently expressed in tumour cells. The objectives of the study were to examine the ... safety and immunogenicity in the first clinical trial of a live recombinant vaccinia virus expressing the E6 and E7 proteins of ...
We observed tissue and intracellular misdistribution of DLG1 when high-risk HPV-18 E7 or E6/E7 proteins were expressed in ... and abundance of DLG1 were also detected in organotypic cultures expressing the low-risk HPV-11 E7 or E6/E7 proteins, ... The viral oncoproteins induce the loss of DLG1 from the cell borders and an increase in the level of DLG1 protein, reflecting ... This report offers new evidence, we believe, of the involvement of HPV proteins in DLG1 expression pattern and our data support ...
Immunofluorescence detection using monoclonal antibodies against E6 or E7 ORF proteins revealed that E6 or E7 proteins of HPV ... Expression and localization of human papillomavirus type 16 E6 and E7 open reading frame proteins in human epidermal ... HPVs belonging to a high risk group have been shown to express two major transforming proteins, E6 and E7. With respect to the ... In the present study, HPV type 16 E6 or E7 open reading frame (ORF) proteins were expressed and localized in human epidermal ...
... but fifteen proteins (~2%) were outliers in this regulatory pattern. Ten demonstrated opposite regulation in E6- and E7- ... Viral E6 and E7 oncogenes cooperate to achieve cell immortalization by a mechanism that is not yet fully understood. Here, ... Two of these were identified as forms of the small heat shock protein HSPB1 (Hsp27). This large-scale analysis provides a ... Most of these changes were qualitatively similar in cells immortalized by E6, E7, or E6/7 expression, indicating convergence on ...
6. Similarities of the CPV-2 and gamma HPV E7 proteins. (A) Phylogenetic analysis of papillomavirus E7 gene sequences. The E7 ... we screened a papillomavirus phylogentic tree based upon the E7 protein sequence (Figure 6A). E7 proteins from 33 HPVs and 15 ... we report that the E7 protein of the malignancy-associated canine papillomavirus type 2 encodes an E7 protein that has serine ... we report that the E7 protein of the malignancy-associated canine papillomavirus type 2 encodes an E7 protein that has serine ...
Vaccine to Target E6 and E7 for Human Papillomavirus (HPV) Associated Cancer - Drug Profile. Product Description. Mechanism Of ... Human Papillomavirus Protein E6 (E6) - Pipeline Review, H1 2018. Summary:. Human Papillomavirus Protein E6 (E6) - The Human ... Human Papillomavirus Protein E6 (E6) - Dormant Products. Human Papillomavirus Protein E6 (E6) - Discontinued Products. Human ... Human Papillomavirus Protein E6 (E6) - Overview. Human Papillomavirus Protein E6 (E6) - Therapeutics Development. Products ...
... first proposed that human papillomaviruses (HPVs) played a role in cervical cancer; in 2008, he shared the Nobel Prize in ... zur Hausen first proposed that human papillomaviruses (HPVs) played a role in cervical cancer (zur Hausen, 1976); in 2008, he ... Papillomaviruses (PVs) were the first viruses recognized to cause tumors and cancers in mammalian hosts by Shope, nearly a ... Papillomaviruses (PVs) were the first viruses recognized to cause tumors and cancers in mammalian hosts by Shope, nearly a ...
RBL2 also interacts with viral ONCOPROTEINS such as POLYOMAVIRUS TUMOR ANTIGENS; ADENOVIRUS E1A PROTEINS; and PAPILLOMAVIRUS E7 ... and ProteinsProteinsNeoplasm ProteinsTumor Suppressor ProteinsRetinoblastoma-Like Protein p130 ... and ProteinsProteinsNuclear ProteinsRetinoblastoma-Like Protein p130. All MeSH CategoriesChemicals and Drugs CategoryAmino ... Retinoblastoma-Like Protein p130. A negative regulator of the CELL CYCLE that undergoes PHOSPHORYLATION by CYCLIN-DEPENDENT ...
  • These results suggest that TGF-β2- and TGF-β-responsive genes are important targets for the HPV-16 E6 and E7 oncoproteins in differentiating cervical keratinocytes. (aacrjournals.org)
  • HPV-related cancers express the viral oncoproteins E6 and E7. (sigmaaldrich.com)
  • The human papillomavirus type 16 E7 protein belongs to a family of nuclear oncoproteins that share amino acid sequences and functional homology. (asm.org)
  • The viral oncoproteins induce the loss of DLG1 from the cell borders and an increase in the level of DLG1 protein, reflecting the pattern observed in cervical lesions. (microbiologyresearch.org)
  • Here, human keratinocytes were immortalized by long-term expression of HPV type 16 E6 or E7 oncoproteins, or both. (biomedcentral.com)
  • This large-scale analysis provides a framework for understanding the cooperation between E6 and E7 oncoproteins in HPV-driven carcinogenesis. (biomedcentral.com)
  • Even these oncoproteins have a low molecular size, they are highly promiscuous and are capable to interact with a whole variety of host cellular regulator proteins to elicit cellular immortalization and ultimately complete malignant transformation. (nih.gov)
  • To avoid reiterations in summarizing the biochemical and molecular biological properties of E6/E7 in terms of their influence on cell cycle control, the present review is mainly an attempt to describe some regulatory principles by which human papillomavirus (HPV) oncoproteins can interfere with apoptosis in order to escape immunological surveillance during progression to cervical cancer. (nih.gov)
  • The high-risk HPV types encode two oncoproteins, E6 and E7, both of which are involved in cellular immortalization ( 20 , 22 , 33 ). (asm.org)
  • 1988-1990, independent Research Scientist at the Ludwig Institute for Cancer Research in London, UK, where he continued his studies on the role of Human Papillomavirus oncoproteins in the development of cervical cancer. (icgeb.org)
  • The development of HPV-induced malignancies requires the activity of two viral oncoproteins, E6 and E7. (icgeb.org)
  • The E6 oncoproteins of the cancer-associated or high-risk human papillomaviruses (HPVs) target the cellular p53 protein. (pubmedcentralcanada.ca)
  • Chemical synthesis of human papillomavirus type 16 E7 oncoprotein: autonomous protein domains for induction of cellular DNA synthesis and for trans activation. (asm.org)
  • The E7 oncoprotein associates with Mi2 and histone deacetylase activity to promote cell growth. (semanticscholar.org)
  • Expression of human papillomavirus type 16 E7 oncoprotein alters keratinocytes expression profile in response to tumor necrosis factor-α. (microbiologyresearch.org)
  • The E7 oncoprotein is approximately 100 amino acids in length and contains two highly conserved regions (CRs), the amino-terminal CR1 and CR2 domains [7] . (prolekarniky.cz)
  • We offer Human Papilloma Virus, type 11, oncoprotein E7 Antibodies for use in common research applications: ELISA, Western Blot. (novusbio.com)
  • Each Human Papilloma Virus, type 11, oncoprotein E7 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
  • Our Human Papilloma Virus, type 11, oncoprotein E7 Antibodies can be used in a variety of model species: Human. (novusbio.com)
  • HPV E7 antibody was raised against hPV oncoprotein E7, type 16. (lsbio.com)
  • The nanotube biosensor proved capable of detecting human ferritin, the primary iron-storing protein of cells, and E7 oncoprotein derived from human papillomavirus. (medindia.net)
  • Cloning and expression of the cDNA for E6-AP, a protein that mediates the interaction of the human papillomavirus E6 oncoprotein with p53. (pubmedcentralcanada.ca)
  • Infection with human papillomavirus type 16 (HPV-16) is the main factor associated with development of cervical cancer ( 42 ). (asm.org)
  • Infection with high-risk human papillomaviruses (HPVs) represents a major risk factor for the development of cervical cancer. (aacrjournals.org)
  • en] Purpose: Infection with oncogenic human papillomavirus (HPV) and HPV-16 in particular is a leading cause of anogenital neoplasia. (ac.be)
  • These findings suggest that the presence of anti‐E7 antibody in serum depends on the staging of cervical cancer and extent of HPV infection. (fujita-hu.ac.jp)
  • BACKGROUND: Human papillomavirus (HPV) infection, especially with type 16 or 18, is associated with cervical cancer. (openrepository.com)
  • Human papillomavirus and genome instability: from productive infection to cancer. (bioportfolio.com)
  • Infection with high oncogenic risk human papillomavirus types is the etiological factor of cervical cancer and a major cause of other epithelial malignancies, including vulvar, vaginal, anal, penile a. (bioportfolio.com)
  • human papillomavirus (HPV) related genital infection leads to cervical cancer development, cervical cancer should be the target of primary prevention through vaccination. (bioportfolio.com)
  • The purpose of this study is to determine if maternal infection with human papillomavirus (HPV) is associated with pregnancy complications including spontaneous preterm delivery (sPTD), se. (bioportfolio.com)
  • The main risk factor for the development of cervical lesions is human papillomavirus (HPV) infection. (bioportfolio.com)
  • Human papillomavirus infection is associated with the development of malignant and benign neoplasms. (bioportfolio.com)
  • Human papillomavirus infection is an infection by human papillomavirus (HPV). (wikipedia.org)
  • An HPV infection is caused by human papillomavirus , a DNA virus from the papillomavirus family, of which over 170 types are known. (wikipedia.org)
  • Cutaneous human papillomavirus (HPV) infection may be a risk factor for squamous cell carcinoma (SCC) of the skin. (aacrjournals.org)
  • Emerging evidence suggests that cutaneous human papillomavirus (HPV) infection may also be a risk factor for SCC ( 3-8 ). (aacrjournals.org)
  • 2018. High-definition analysis of host protein stability during human cytomegalovirus infection reveals antiviral factors and viral evasion mechanisms . (cardiff.ac.uk)
  • Many studies have suggested a possible link between breast cancer pathogenesis and viral infection, particularly mouse mammary tumour virus, simian virus 40, Epstein-Barr virus, and human papillomavirus (HPV). (springer.com)
  • These proteins are then presented to the immune system, which alerts the body that an infection and cancer is present. (uchospitals.edu)
  • Methylation of twelve CpGs in human papillomavirus type 16 (HPV16) as an informative biomarker for the triage of women positive for HPV16 infection. (yale.edu)
  • Numerous versions of human papillomavirus (HPV) therapeutic vaccines designed to treat individuals with established HPV infection, including those with cervical intraepithelial neoplasia (CIN), are in development because approved prophylactic vaccines are not effective once HPV infection is established. (asm.org)
  • As opposed to searching for the HPV antibody or cell-mediated immine responses after initial infection, the nanotube sensor can track the HPV protein directly. (medindia.net)
  • More than 90% of cervical cancers are associated with human papillomavirus (HPV) infection ( 4 ). (aacrjournals.org)
  • Clifford GM, de Vuyst H, Tenet V, Plummer M, Tully S, Franceschi S. Effect of HIV infection on human papillomavirus types causing invasive cervical cancer in Africa. (springer.com)
  • Human papillomavirus (HPV) infection is one candidate for the development of SNP for its epithelial cell trophism, hyperproliferative effect, and the induction of immune-modulatory molecules as HLA-G. We enrolled 10 patients with SNP without concomitant allergic diseases (SNP-WoAD), 10 patients with SNP and suffering from allergic diseases (SNP-WAD), and 10 control subjects who underwent rhinoplasty. (hindawi.com)
  • Viral infection has been postulated to be one important aetiological factor in the pathogenesis, progression, and recurrence of nasal polyps [ 4 ], with human papillomavirus (HPV) infection as a candidate for the development of nasal polyps [ 5 , 6 ]. (hindawi.com)
  • The HPV-16 E6 and E7 proteins are highly expressed in differentiating keratinocytes, where they inactivate the p53 and retinoblastoma (pRb) proteins, two important transcriptional regulators. (aacrjournals.org)
  • The latter inactivates the tumor suppressor protein retinoblastoma (Rb), which leads to the overexpression of p16(INK4) protein, providing unique Ags for therapeutic HPV-specific cancer vaccination. (sigmaaldrich.com)
  • Adenovirus E1A, simian virus 40 tumor antigen, and human papillomavirus E7 protein share the capacity to disrupt the interaction between transcription factor E2F and the retinoblastoma gene product. (semanticscholar.org)
  • These sequences are also required for these proteins to bind to the retinoblastoma gene product (pRb). (semanticscholar.org)
  • Targeting the retinoblastoma protein by MC007L, gene product of the molluscum contagiosum virus: detection of a novel virus-cell interaction by a member of the poxviruses. (semanticscholar.org)
  • E7 binds to the retinoblastoma protein (Rb) and disrupts the Rb/E2F/HDAC complex. (biomedcentral.com)
  • The high-risk HPV E6 and E7 proteins cooperate to immortalize primary human cervical cells and the E7 protein can independently transform fibroblasts in vitro, primarily due to its ability to associate with and degrade the retinoblastoma tumor suppressor protein, pRb. (prolekarniky.cz)
  • Papillomavirus E7 proteins have been shown to interact with various regulators of the cell cycle including RETINOBLASTOMA PROTEIN and certain cyclin-dependent kinase inhibitors. (bioportfolio.com)
  • E7 complexes with members of the retinoblastoma family and inactivates their growth-suppressing functions ( 27 ). (asm.org)
  • This E7 activity is independent of retinoblastoma protein or AP-2 degradation. (scialert.net)
  • Hume AJ, Kalejta RF (2009) Regulation of the retinoblastoma proteins by the human herpesviruses. (springer.com)
  • A yeast two-hybrid screen was employed to identify human proteins that specifically bind the amino-terminal 400 amino acids of the retinoblastoma (Rb) protein. (asm.org)
  • Functional biomarkers p16, Ki-67, p53, retinoblastoma protein cytokeratin (CK)14 and CK13, help in the assessment of an individual CIN's lesion's potential for progression and regression. (bmj.com)
  • This activates the retinoblastoma protein (pRB) and, through incompletely understood events, arrests the cell division cycle. (asm.org)
  • The only known effector of the arrest is the retinoblastoma protein (pRB), consistent with evidence that a major role of cdk4/6 is to phosphorylate and inactivate pRB ( 98 ). (asm.org)
  • Although the retinoblastoma protein (Rb) functions as a checkpoint in the cell cycle, it also regulates differentiation. (biologists.org)
  • The retinoblastoma protein (Rb) belongs to a family of proteins called 'pocket proteins', which also includes p107 and p130. (biologists.org)
  • The beta human papillomaviruses (HPVs) are subdivided into 5 species (beta-1 to beta-5), and they were first identified in the skin. (asm.org)
  • HPVs belonging to a high risk group have been shown to express two major transforming proteins , E6 and E7. (bvsalud.org)
  • Screening of HPV genome sequences revealed that the LXSXE motif of the canine E7 protein was also present in the gamma HPVs and we demonstrate that the gamma HPV-4 E7 protein also binds pRb in a similar way. (prolekarniky.cz)
  • It appears, therefore, that the type 2 canine PV and gamma-type HPVs not only share similar properties with respect to tissue specificity and association with immunosuppression, but also the mechanism by which their E7 proteins interact with pRb. (prolekarniky.cz)
  • Human papillomaviruses (HPVs) mediate the initiation and maintenance of cervical cancer [1] , [2] . (prolekarniky.cz)
  • Human papillomaviruses (HPVs) are small, non-enveloped icosahedral viruses of 55 nm containing a circular, ≈8-kb double-stranded DNA genome condensed by cellular histones. (frontiersin.org)
  • There is still controversy in the scientific field about whether certain types of cutaneous human papillomaviruses (HPVs) are causally involved in the development of non-melanoma skin cancer (NMSC). (frontiersin.org)
  • Human Papillomaviruses (HPVs) are the causative agents of a number of human tumours, including cervical cancer, which is a major cause of cancer related death in women in many parts of the developing world. (icgeb.org)
  • Most of the oncogenic potential of HR-HPVs resides on the PDZ-binding domain of E6 protein. (aacrjournals.org)
  • These HR-HPVs owe their oncogenic potential to the constitutive expression of the E6 and E7 oncogenes whose products enhance cell proliferation and perturb cell differentiation through several mechanisms ( 3 ). (aacrjournals.org)
  • Since their discovery as the etiologic agents of cervical cancer in the mid-1970s, human papillomaviruses (HPVs) have been linked with a growing number of epithelial-derived tumors, including head and neck squamous cell carcinomas. (springer.com)
  • Transmission of high-risk human papillomavirus (HPV) between parents and infant: a prospective study of HPV in families in Finland. (springer.com)
  • Impact of connexin32 deletion on E7 or RET/PTC3 oncogene-driven growth and neoplastic transformation of the thyroid gland. (semanticscholar.org)
  • Expression of the E7 oncogene of HPV-16 induces S phase entry of mammalian cells in the presence of antiproliferative signals. (nih.gov)
  • Silencing of E7 oncogene restores functional E-cadherin expression in human papillomavirus 16-transformed keratinocytes. (microbiologyresearch.org)
  • ONCOGENE PROTEINS from papillomavirus that deregulate the CELL CYCLE of infected cells and lead to NEOPLASTIC CELL TRANSFORMATION. (bioportfolio.com)
  • We further demonstrated that HeLa-SFCs expressed a higher level (6.9-fold) of the human papillomavirus oncogene E6, compared with that of parental HeLa cells. (nature.com)
  • Binding of YY1 to the proximal fragment of the human papillomavirus type 18 (HPV-18) upstream regulatory region (URR) activates the oncogene expression of HPV-18 in HeLa cells, whereas in HepG2 cells this expression is repressed by YY1. (biomedsearch.com)
  • We show that E7-specific antibodies and CD4 T helper responses can be obtained by vaccinating E7 transgenic mice, although a CTL response was not detected. (aacrjournals.org)
  • By Western blot technique, 519 samples of human sera were tested for the presence of antibodies to the human papillomavirus (HPV) type 16 proteins E4 and E7 that had been expressed in Escherichia coli as fusion proteins. (eurekamag.com)
  • Within the control population, 18.1% of them had antibodies that reacted with the E4 protein, and 3.9% of them had antibodies that reacted with the E7 protein. (eurekamag.com)
  • Antibodies against the HPV16 E7 protein were found 14 times more frequently in patients with cervical cancer, compared with age- and sex-matched controls (P less than .00001). (eurekamag.com)
  • From these data, we concluded that anti-E4 antibodies may be correlated with virus replication and that anti-E7 antibodies may represent a marker for cervical cancer development. (eurekamag.com)
  • Hybridoma cell line producing CamVir-4 antibodies raised against E7 protein of human papillomavirus 16 which are mouse monoclonal antibodies. (cam.ac.uk)
  • Immunofluorescence detection using monoclonal antibodies against E6 or E7 ORF proteins revealed that E6 or E7 proteins of HPV type 16 were located in the cytoplasm of RHEK-1 cells . (bvsalud.org)
  • Impact of Naturally Occurring Variation in the Human Papillomavirus (HPV) 58 Capsid Proteins on Recognition by Type-Specific Neutralizing Antibodies. (bioportfolio.com)
  • We investigate the impact of variation within the major (L1) and minor (L2) capsid proteins of HPV58 on susceptibility to neutralizing antibodies. (bioportfolio.com)
  • Impact of naturally occurring variation in the human papillomavirus 52 capsid proteins on recognition by type-specific neutralising antibodies. (bioportfolio.com)
  • Antibodies to human papillomavirus type 16 E7 related to clinicopathological data in patients with cervical carcinoma. (bmj.com)
  • AIMS--To investigate the correlation between antibodies to the transforming protein E7 of human papillomavirus (HPV) type 16 and clinicopathological indices in women with cervical squamous carcinoma. (bmj.com)
  • METHODS--A synthetic peptide of the HPV type 16 E7 protein (amino acids 6 to 35) was used to screen sera from 29 children, 130 women with cervical intraepithelial neoplasia, 443 women with cervical cancer, and 222 controls, for antibodies against this viral antigen. (bmj.com)
  • Bivariate analysis of the data on the presence of anti-E7/6-35 antibodies in the pretreatment sera from these patients and clinicopathological indices showed a significant correlation between the presence of anti-E7/6-35 antibodies and the size of the lesion (p = 0.0009), histological grade (p = 0.0031), and lymph node metastasis (p = 0.01). (bmj.com)
  • In addition, the Cox regression model, analysing four risk factors which can be determined before treatment, showed a significant correlation between the presence of anti-E7/6-35 antibodies and a worse prognosis (p = 0.003). (bmj.com)
  • CONCLUSIONS--The presence of antibodies against E7/6-35 in pretreatment sera from patients with cervical carcinoma correlates with the size of the lesions, lymph node involvement, and a worse prognosis. (bmj.com)
  • Over the last two decades, a number of studies have focused on the use of Lactococcus lactis as a mucosal delivery vector for therapeutic proteins and antigens ( 4 - 6 ). (spandidos-publications.com)
  • Because most identified tumor-associated antigens are self antigens rather viral antigens, we used E7 transgenic mice to evaluate the E7-based vaccine in conditions where E7 is a self antigen. (aacrjournals.org)
  • The E1A protein of adenovirus, large-T antigens of simian virus 40 (SV40) and polyomaviruses, and the E7 protein of human papillomaviruses all form physical complexes with the Rb protein, and such interactions abrogate Rb-mediated growth suppression ( 16 , 22 , 23 , 63 , 85 ). (asm.org)
  • Furthermore, L. lactis-produced antigens are presented to the immune system in particulate form, which is postulated to evoke stronger mucosal response than soluble proteins [17, 18]. (thefreelibrary.com)
  • Since the 1990s, several workers have investigated the use of bacteria as E7 antigen delivery vehicles to elicit an immune response against HPV-16 ( 14 , 22 ). (asm.org)
  • However, it is unknown whether the same results may be triggered through immunization of animals with L. lactis simultaneously carrying protein antigen and cytokine DNA. (spandidos-publications.com)
  • In the present study, the use of a single L. lactis strain, to co‑administer protein antigen and adjuvant DNA, successfully induced an antigen‑specific immune response. (spandidos-publications.com)
  • Because this protein is a good example of a tumor-associated antigen, we have used E7 as a model antigen to test the potential of an experimental vaccine as an immunotherapeutic approach. (aacrjournals.org)
  • Two responding patients generated long-term T-cell immunity toward the vaccine antigen and E7 as well as a weak H. influenzae protein D (PD)-directed CD4(+) response. (ac.be)
  • The adenovirus E1A gene product, the simian virus 40 large tumor antigen, and the human papillomavirus E7 protein share a short amino acid sequence that constitutes a domain required for the transforming activity of these proteins. (semanticscholar.org)
  • 4 . A method according to any one of claims 1 to 3 wherein the antigen is a protein. (google.com)
  • Exogenously introduced proteins, such as those used in our assays, may be taken up in endocytic compartments where they are degraded and processed for antigen presentation by major histocompatibility complex class II molecules. (asm.org)
  • The human leukocyte antigen (HLA)- DRB1*13 allele frequency is lower in women with cervical carcinoma than in the general population, suggesting that this allele could exert a protective effect against progression of cervical intraepithelial neoplasia (CIN) associated with human papillomaviruses (HPV). (lww.com)
  • In the last several decades lactococci have been exploited as hosts for expression of heterologous antigen proteins, including those of therapeutic and prophylactic activity [1, 2]. (thefreelibrary.com)
  • p>This indicates the type of evidence that supports the existence of the protein. (uniprot.org)
  • p>This subsection of the 'Function' section specifies the position(s) and type(s) of zinc fingers within the protein. (uniprot.org)
  • The report provides comprehensive information on the Human Papilloma Virus (HPV) E7 Protein, targeted therapeutics, complete with analysis by indications, stage of development, mechanism of action (MoA), route of administration (RoA) and molecule type. (reportsnreports.com)
  • The early genes E6 and E7 of human papillomavirus type 16 (HPV16) are consistently and exclusively expressed in HPV16-induced cancer lesions and play major roles in the development and maintenance of the malignant phenotype. (aacrjournals.org)
  • Reference : Phase I/II trial of immunogenicity of a human papillomavirus (HPV) type 16 E7 protein. (ac.be)
  • Sera were examined for the presence of antibody against E7 protein of human papillomavirus type 16 (HPV‐16) by Western blot analysis using the bacterially derived unfused protein. (fujita-hu.ac.jp)
  • Human papillomavirus type 16 (HPV-16) virus-like particle L1-specific CD8+ cytotoxic T lymphocytes (CTLs) are equally effective as E7-specific CD8+ CTLs in killing autologous HPV-16-positive tumor cells in cervical cancer patients: implications for L1 dendritic cell-based therapeutic vaccines. (openrepository.com)
  • In the present study, HPV type 16 E6 or E7 open reading frame (ORF) proteins were expressed and localized in human epidermal keratinocytes (RHEK-1) using the vaccinia virus as an expression vector. (bvsalud.org)
  • In the current study, we report that the E7 protein of the malignancy-associated canine papillomavirus type 2 encodes an E7 protein that has serine substituted for cysteine in the LXCXE motif. (prolekarniky.cz)
  • We investigated the impact of naturally occurring variation within the major (L1) and minor (L2) capsid proteins on the antigenicity of human papillomavirus (HPV) type 52 (HPV52). (bioportfolio.com)
  • A type of human papillomavirus especially associated with malignant tumors of the genital and RESPIRATORY MUCOSA. (bioportfolio.com)
  • E2 has been best studied for bovine papillomavirus type 1 (BPV-1), which encodes full-length E2 protein (E2TA), as well as two truncated forms of E2, E2TR and E8/E2, an alternatively spliced (28 kDa) protein ( 21 ). (asm.org)
  • The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. (genecards.org)
  • Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). (genecards.org)
  • Cytotoxic T-lymphocyte (CTL) responses to E6 and E7 were previously shown to be more commonly detectable in human papillomavirus type 16 (HPV-16)-positive women without squamous intraepithelial neoplasia (SIL) than in HPV-16-positive women with SIL (M. Nakagawa, D. P. Stites, S. Farhat, J. R. Sisler, B. Moss, F. Kong, A. B. Moscicki, and J. M. Palefsky, J. Infect. (asm.org)
  • In a previous study ( 13 ), we showed that cytotoxic T-lymphocyte (CTL) responses to HPV type 16 (HPV-16) E6 and E7 proteins appear to be important in the prevention of SIL in that responses to both E6 and E7 proteins were more commonly found in HPV-16-positive women without SIL than in HPV-16-positive women with SIL. (asm.org)
  • Rb is abruptly dephosphorylated at the end of mitosis probably by a type I protein phosphatase activated in anaphase ( 54 , 55 , 65 ). (asm.org)
  • In contrast, DMhigh APC significantly promoted the presentation of DQ2-restricted epitopes derived intracellularly from inactivated HSV type 2, influenza hemagglutinin, and human papillomavirus E7 protein. (stanford.edu)
  • As human papillomavirus 16 (HPV-16) is the most commonly detected type worldwide, all versions of HPV therapeutic vaccines contain HPV-16, and some also contain HPV-18. (asm.org)
  • A designation of a new type was created whenever a full-length papillomavirus clone was described which was at least 10% dissimilar from any other known papillomavirus type ( 6 ). (asm.org)
  • E6-AP, translated in vitro, has the following properties: (i) it associates with wild-type p53 in the presence of the HPV16 E6 protein and simultaneously stimulates the association of E6 with p53, (ii) it associates with the high-risk HPV16 and HPV18 E6 proteins in the absence of p53, and (iii) it induces the E6- and ubiquitin-dependent degradation of p53 in vitro. (pubmedcentralcanada.ca)
  • Dürst M, Dzarlieva-Petrusevska RT, Boukamp P, Fusenig NE, Gissmann L. Molecular and cytogenetic analysis of immortalized human primary keratinocytes obtained after transfection with human papillomavirus type 16 DNA. (pubmedcentralcanada.ca)
  • Grossman SR, Mora R, Laimins LA. Intracellular localization and DNA-binding properties of human papillomavirus type 18 E6 protein expressed with a baculovirus vector. (pubmedcentralcanada.ca)
  • In the present study, we tested the immunogenicity of a fusion protein (PD-E7) comprising a mutated HPV-16 E7 linked to the first 108 amino acids of Haemophilus influenzae protein D, formulated in the GlaxoSmithKline Biologicals adjuvant AS02B, in patients bearing oncogenic HPV-positive cervical intraepithelial neoplasia (CIN). (ac.be)
  • The occurrence rates of anti‐E7 antibody against HPV‐16 were 14.1% (10/71) in cervical cancer patients, 0% (0/48) in cervical intraepithelial neoplasia patients, and 0% (0/41) in female non‐malignant patients. (fujita-hu.ac.jp)
  • It has been proven that AIN, as in the case of cervical intraepithelial neoplasia (CIN) and in vulvar intraepithelial neoplasia (VIN), is related with the human papillomavirus (HPV) (1) in more than 90% of cases, particularly with serotypes 16 (85%) and 18 (7%) (2). (isciii.es)
  • Down-regulation of TGF-β2 was biologically relevant because the addition of recombinant cytokine (10-200 pg/ml) to E6/E7-expressing cells restored expression of TGF-β-responsive genes, inhibited growth of keratinocytes, and decreased immortalization by E6 and E7. (aacrjournals.org)
  • A recombinant vaccinia virus encoding human papillomavirus types 16 and 18, E6 and E7 proteins as immunotherapy for cervical cancer. (openrepository.com)
  • The objectives of the study were to examine the clinical and environmental safety and immunogenicity in the first clinical trial of a live recombinant vaccinia virus expressing the E6 and E7 proteins of HPV 16 and 18 (TA-HPV). (openrepository.com)
  • Safety and immunogenicity of TA-HPV, a recombinant vaccinia virus expressing modified human papillomavirus (HPV)-16 and HPV-18 E6 and E7 genes, in women with progressive cervical cancer. (openrepository.com)
  • In our HPV-CTL assay, recombinant fusion protein containing HPV-16 E6 or E7 was used for in vitro stimulation of effector cells. (asm.org)
  • The system allows efficient but controlled expression of recombinant genes in L. lactis cells, including toxic proteins, on lab and industrial scales [19,20]. (thefreelibrary.com)
  • CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. (rndsystems.com)
  • Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (rndsystems.com)
  • In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)
  • The incidence of human papillomavirus (HPV)-related head and neck squamous cell carcinoma has increased in recent decades, though HPV prevention vaccines may reduce this rise in the future. (sigmaaldrich.com)
  • In this study, we analysed the contribution of HPV proteins to the changes in DLG1 expression in the squamous epithelium. (microbiologyresearch.org)
  • Human papillomavirus (HPV) infections are quite common and cause a variety of benign proliferations including cutaneous warts, venereal warts, genital squamous intraepithelial lesions, and orolaryngeal and -pharyngeal papillomas, as well as other types of hyperkeratoses ( 41 ). (asm.org)
  • The link between human papillomavirus (HPV) and the development of squamous intraepithelial lesions (SIL) as well as cervical cancer is well known. (asm.org)
  • Background: Human papillomaviruses (HPV) may play an important role as one of the possible etiologies of oral squamous cell carcinoma (OSCC). (koreascience.or.kr)
  • Papillomaviruses infect squamous epithelia as skin and mucosae. (hindawi.com)
  • E6 and E7 bind the products of tumour suppressor genes, p53 and Rb1, respectively, modifying or inactivating their normal functions. (nih.gov)
  • The Rb1 and p53 genes are deleted or mutated in several cancers and both proteins regulate the transcription of genes involved in cell cycle progression control. (nih.gov)
  • We show that E6 and E7 decrease expression of TGF-β2 mRNA and alter expression of multiple TGF-β-responsive genes involved in cell cycle regulation, apoptosis, and tissue remodeling. (aacrjournals.org)
  • The E7 protein binds and sequesters pRb and directs its ubiquitin-mediated proteolysis [6] , thereby altering E2F-regulated genes and allowing cells to enter the S phase of the cell cycle. (prolekarniky.cz)
  • Viral early genes E6 and E7 from high-risk HPV types are responsible for the transformation of epithelial cells, and their continuous expression is essential for ongoing cervical cancer cell survival as they function as oncogenes. (nature.com)
  • We performed human papillomavirus (HPV) typing and identified somatically mutated genes using exome and ultra-deep targeted sequencing with confirmation in samples from Mexico. (aacrjournals.org)
  • These data provide the first evidence that Rb and Rb-related proteins can directly regulate DNA replication and that components of licensing factor are targets of the products of tumor suppressor genes. (asm.org)
  • The antitumor effects of the vaccination occurred through an E7‑specific cytotoxic T‑lymphocyte response. (spandidos-publications.com)
  • We show that vaccination with the E7 protein, formulated in the SmithKline Beecham Biologicals proprietary adjuvants (SBAS 1 and SBAS 2), leads to the rejection of pre-established tumors. (aacrjournals.org)
  • Numerous preclinical studies have demonstrated the therapeutic potential of E7-directed vaccination strategies in mice tumour models. (ac.be)
  • Novel oligomannose liposome-DNA complex DNA vaccination efficiently evokes anti-HPV E6 and E7 CTL responses. (openrepository.com)
  • The health impact of human papillomavirus vaccination in the situation of primary human papillomavirus screening: A mathematical modeling study. (bioportfolio.com)
  • Human papillomavirus (HPV) vaccination and the implementation of primary HPV screening in the Netherlands will lead to a lower cervical disease burden. (bioportfolio.com)
  • A randomized, observer-blind non-inferiority trial to evaluate alternative human papillomavirus (HPV) vaccination schedules in young females in West Africa. (bioportfolio.com)
  • Papillomaviruses-to vaccination and beyond. (springer.com)
  • We have used cDNA expression arrays to identify global alterations in gene expression induced by E6 and E7 in differentiating cultures of human cervical keratinocytes. (aacrjournals.org)
  • Concordant Induction of Cyclin E and p21cip1 in Differentiated Keratinocytes by the Human Papillomavirus E7 Protein Inhibits Cellular and Viral DNA Synthesis -- Jian et al. (aacrjournals.org)
  • HPV49, HPV75, and HPV76 E6 and E7 (E6/E7), but not HPV115 E6 and E7, efficiently inactivate the p53 and pRb pathways and immortalize or extend the life span of human foreskin keratinocytes (HFKs). (asm.org)
  • Expression of 170 (23%) of the protein features changed significantly in immortalized cells compared to primary keratinocytes. (biomedcentral.com)
  • Expression of either high-risk HPV E6 or E7 in human keratinocytes extends the period of growth prior to senescence well beyond normal. (biomedcentral.com)
  • Yoshimatsu Y, Nakahara T, Tanaka K, Inagawa Y, Narisawa-Saito M, Yugawa T, Ohno S I, Fujita M, Nakagama H, and Kiyono T: Roles of the PDZ-binding motif of HPV 16 E6 protein in oncogenic transformation of human cervical keratinocytes. (go.jp)
  • HPV16 E6 and E7 proteins cooperate to immortalize human foreskin keratinocytes. (pubmedcentralcanada.ca)
  • Recognizes Human Papilloma Virus Type16 (HPV16) E7 Protein. (lsbio.com)
  • The E6/p53 and E7/Rb1 interactions result in a deregulation of the cell cycle with loss of control of crucial cellular events, such as DNA replication, DNA repair and apoptosis. (nih.gov)
  • trans Activation does not require protein synthesis, implying a mechanism that involves interaction with a preexisting cellular factor(s). (asm.org)
  • E7 trans activates the adenovirus E2 promoter but not other E1A-inducible viral promoters, suggesting the possibility that E7 trans activation involves interaction, directly or indirectly, with cellular transcription factor E2F. (asm.org)
  • Comparative analysis of cellular gene expression patterns of HFKs containing E6 and E7 from HR HPV16, beta-3 HPV types, and beta-2 HPV38 further highlights the functional similarities of HR HPV16 and beta-3 HPV49, HPV75, and HPV76. (asm.org)
  • The high-risk HPV E6 binds to several cell targets, including p53, Myc, E6AP, PDZ proteins and other cellular proteins to alter apoptotic/growth regulatory pathways and induce cellular telomerase activity [5] . (prolekarniky.cz)
  • A common feature shared between several human cancer-associated viruses, such as Epstein-Barr virus, Hepatitis B virus and Hepatitis C virus, and Human papillomavirus (HPV) is the ability to reduce the expression of cellular E-cadherin. (scialert.net)
  • A number of DNA and RNA viruses encode proteins that target critical cell cycle regulators to achieve cellular conditions that are beneficial for viral replication. (springer.com)
  • Human papillomavirus (HPV) specifically targets p53, along with phosphorylated Rb and other proteins, to maintain a cellular environment that promotes viral replication ( 2 ). (aacrjournals.org)
  • Complex formation between E6 and p53 requires an additional cellular factor, designated E6-AP (E6-associated protein), which has a native and subunit molecular mass of approximately 100 kDa. (pubmedcentralcanada.ca)
  • We have shown previously that there is increased SUMOylation during keratinocyte differentiation, suggesting that the levels of E2 protein may be tied to changes in the cellular SUMOylation state during differentiation. (portlandpress.com)
  • In addition to itself being regulated by SUMOylation, E2 appears to influence the SUMOylation state of one of its binding partners, the cellular transcription factor, C/EBP (CCAAT/enhancer-binding protein). (portlandpress.com)
  • Recent proteomics approaches indicate that numerous cellular proteins, primarily in the nucleus, are SUMOylated [ 2 , 3 ]. (portlandpress.com)
  • Nonetheless, most of the known SUMOylation targets are transcription factors or other proteins involved in chromatin structure, regulation and expression [ 4 ], supporting a fundamental role for this modification system in the cellular life cycle. (portlandpress.com)
  • It targets the E6 and E7 proteins of human papillomavirus (HPV) types 16 and 18, a common cause of cervical cancers. (uchospitals.edu)
  • Rb-1 encodes a ubiquitously expressed set of nuclear proteins, termed p105-Rb, that are subject to cyclical waves of phosphorylation by cyclin-dependent kinases ( 7 , 8 , 51 , 55 , 60 , 80 , 83 ). (asm.org)
  • The TP53 gene encodes the p53 protein, which is involved in a series of pathways, such as apoptosis, transcription, DNA repair, maintenance of genomic stability, and cell cycle control ( 1 ). (aacrjournals.org)
  • The locus encodes a second protein, p14 ARF (ARF), from an alternative reading frame ( 19 , 63 , 81 , 100 ). (asm.org)
  • The L1 gene encodes a major capsid protein, while the L2 gene encodes a minor capsid protein. (asm.org)
  • HPV late gene that encodes the major capsid protein of the virus. (springer.com)
  • Abnormal aggregation of proteins induces neuronal cell loss in neurodegenerative disorders such as Alzheimer's Disease, Creutzfeldt-Jakob Disease and Parkinson's Disease. (bioportfolio.com)
  • To date, cell culture experiments have shown that the exogenous expression of HR-E6 proteins induces the immortalization of human mammary epithelial cells ( 6 ). (aacrjournals.org)
  • Viral E6 and E7 oncogenes cooperate to achieve cell immortalization by a mechanism that is not yet fully understood. (biomedcentral.com)
  • The research identified papillomavirus oncogenes and the molecular mechanisms of their damaging effects. (wikipedia.org)
  • Their pathogenesis involves polymerization of actin jet trails and invasion of M cells, as well as a protein synthesis inhibiting toxin. (studystack.com)
  • This work is considered fundamental for the understanding of the pathogenesis of papillomaviruses at the molecular level. (wikipedia.org)
  • Accordingly, HIV pathogenesis has been causally associated with such peptide sharing (i.e., with autoimmune phenomena due to molecular mimicry between viral and host proteins) (6-11). (bioscience.org)
  • Mus musculus Papillomavirus 1: a New Frontier in Animal Models of Papillomavirus Pathogenesis. (helsinki.fi)
  • p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence. (uniprot.org)
  • Transgenic mice, which constitutively and specifically express the E7 HPV16 gene in the thyroid epithelium, rapidly develop thyroid goiters and, after several months, thyroid carcinomas. (aacrjournals.org)
  • We show here that E7 can antagonize the ability of p27KIP1 to block cyclin E-associated kinase in vitro and to inhibit transcription from the cyclin A gene in transfection experiments. (nih.gov)
  • The human papillomavirus (HPV) E2 protein is an important regulator of viral E6 and E7 gene expression. (asm.org)
  • E6 and E7 function to stimulate cell proliferation and do so by interfering with the function of regulatory proteins in cells, including the p53 and pRb tumor suppressor gene products ( 15 , 32 , 34 , 50 ). (asm.org)
  • The protein encoded by this gene is a member of the keratin gene family. (genecards.org)
  • KRT7 (Keratin 7) is a Protein Coding gene. (genecards.org)
  • Howley and his co-workers created gene maps of many species of papillomaviruses and analyzed their transcription patterns and systems of transcription regulation. (wikipedia.org)
  • Polymorphisms in the TP53 gene have been hypothesized to modify the conformation of the p53 protein, affecting the ability of HPV to bind and degrade p53 and thus potentially acting early in cervical carcinogenesis. (aacrjournals.org)
  • HPV early gene encoding a protein that regulates viral transcription. (springer.com)
  • In the case of papillomaviruses, gene clusters match well our knowledge on viral biology and life cycle, illustrating the potential of our approach. (biomedcentral.com)
  • Three patients (one with endometrial cancer, one with breast cancer, and one male patient with colon polyp) out of 115 patients with tumors in organs other than the cervix, had antibody against E7 protein of HPV‐16. (fujita-hu.ac.jp)
  • Sixty‐nine percent (9/13) of patients with HPV‐16 DNA in cancers had the antibody and all the patients with stages II, III, and IV cervical cancer (8/8) harboring HPV‐16 DNA showed the presence of the antibody against E7 protein of HPV‐16. (fujita-hu.ac.jp)
  • In contrast, only 20% (1/5) of cervical cancer patients with stage Ia or Ib harboring HPV‐16 DNA showed positive for the anti‐E7 antibody in sera. (fujita-hu.ac.jp)
  • With the aim of identifying new proteins involved in the regulation of mitosis, we used an antibody against phosphotyrosine to analyze proteins from synchronized human and hamster cells. (biomedsearch.com)
  • HPV E7 antibody LS-C528510 is a biotin-conjugated mouse monoclonal antibody to HPV E7 from human and hpv16. (lsbio.com)
  • In contrast to live infections in which an acute CD8 + T cell response is often elicited in the absence of CD4 + T cell help, CD8 + T cell responses against inert protein Ags, peptide-pulsed dendritic cells (DCs), or Ags expressed from replication-deficient viral vectors are usually absolutely dependent on CD4 + T cell help ( 4 - 8 ). (jimmunol.org)
  • In HPV, this substitution in E7 abrogates pRb binding and degradation. (prolekarniky.cz)
  • Finally, while the CR1 and CR2 domains of HPV E7 are sufficient for degradation of pRb, the C-terminal region of canine E7 was also required for pRb degradation. (prolekarniky.cz)
  • High-risk HPV E6 protein can complex with p53 and, as a result, promotes the ubiquitination and degradation of p53 ( 38 ). (asm.org)
  • 2015. Plasma membrane profiling defines an expanded class of cell surface proteins selectively targeted for degradation by HCMV US2 in cooperation with UL141 . (cardiff.ac.uk)
  • Driven by findings that human papillomavirus (HPV)-induced degradation of p53 differs by a TP53 polymorphism at codon 72 (Pro72Arg), past studies of TP53 genetic variants and cervical cancer have focused on this nonsynonymous polymorphism, with mixed results. (aacrjournals.org)
  • In the absence of Wnt ligands, β-catenin forms a "degradation complex" with kinases and scaffold proteins, such as glycogen synthase kinase-3β (GSK3β), casein kinase 1 and 2 (CK1 and CK2), adenomatous polyposis coli (APC), and Axin2. (aacrjournals.org)
  • The association of E6 with p53 leads to the specific ubiquitination and degradation of p53 in vitro, suggesting a model by which E6 deregulates cell growth control by the elimination of the p53 tumor suppressor protein. (pubmedcentralcanada.ca)
  • Energy requirement for degradation of tumor-associated protein p53. (pubmedcentralcanada.ca)
  • In particular, E7 can bypass G0/G1 arrest in response to both serum withdrawal and loss of cell adhesion, two experimental conditions in which cell cycle progression is accompanied by elevated levels of the cdk inhibitor p27KIP1. (nih.gov)
  • Differential expression of the human homologue of Drosophila discs large oncosuppressor in histologic samples from human papillomavirus-associated lesions as a marker for progression to malignancy. (microbiologyresearch.org)
  • Over 60 different types of human papillomavirus (HPV) have been identified, and they are classified into high and low risk groups based on the risk for malignant progression of HPV associated lesions. (bvsalud.org)
  • Integration of the HPV genome into a host cell chromosome is a frequent event during malignant progression and it may play a significant role in dysregulated expression of the HPV E6 and E7 proteins [4] . (prolekarniky.cz)
  • E2 can repress the viral promoter for E6 and E7 expression as well as block progression of the cell cycle in cancer cells harboring the DNA of "high-risk" HPV types. (asm.org)
  • Changes in protein tyrosine phosphorylation are known to be important for regulating cell cycle progression. (biomedsearch.com)
  • Although all these viruses are termed 'DNA tumour viruses' and have similar in vitro transforming activities, Human Papillomavirus is the only one so far clearly involved in human cancer. (nih.gov)
  • Papillomaviruses (PVs) were the first viruses recognized to cause tumors and cancers in mammalian hosts by Shope, nearly a century ago ( Shope and Hurst, 1933 ). (frontiersin.org)
  • The papillomaviruses are small DNA viruses that infect a variety of mammalian species, including humans. (asm.org)
  • The designation of papillomaviruses as the family Papillomaviridae was created in the seventh report of the International Committee for the Taxonomy of Viruses ( 5 ). (asm.org)
  • The detection can be read in real time, instead of after days or weeks of laboratory analysis, meaning the nanotube molecular imprinting technique could pave the way for biosensors capable of detecting human papillomavirus or other viruses weeks sooner than available diagnostic techniques currently allow. (medindia.net)
  • Papillomaviruses are a large group of small DNA viruses that infect the skin, leading to benign lesions (warts) that in some cases can progress to malignancy. (portlandpress.com)
  • Consistent with the nuclear predominance of SUMOylation, most of the nuclear DNA viruses (parvovirus, adenovirus, papovavirus and herpesvirus) have viral proteins that are SUMOylated and/or interact with the SUMOylation machinery, as does HIV and several other RNA viruses [ 5 ]. (portlandpress.com)
  • In contrast, E6 and E7 did not alter expression of TGF-β1 and TGF-β3. (aacrjournals.org)
  • As observed for HR HPV16, cell cycle deregulation mediated by beta-3 HPV E6/E7 expression leads to p16 INK4a accumulation, whereas no p16 INK4a was detected in beta-2 HPV38 E6/E7 HFKs. (asm.org)
  • The expression profiles of these four HPV HFKs show some similarities and diverge substantially from those of beta-3 HPV115 E6/E7 and beta-2 HPV38 E6/E7 HFKs. (asm.org)
  • This report offers new evidence, we believe, of the involvement of HPV proteins in DLG1 expression pattern and our data support previous observations regarding DLG1 expression in cervical lesions. (microbiologyresearch.org)
  • Proteomic profiling was used to compare expression levels for 741 discrete protein features. (biomedcentral.com)
  • Most of these changes were qualitatively similar in cells immortalized by E6, E7, or E6/7 expression, indicating convergence on a common phenotype, but fifteen proteins (~2%) were outliers in this regulatory pattern. (biomedcentral.com)
  • We demonstrate that expression of HPV16 E6 and E7 from a heterologous promoter that is not regulated by E2 rescues HeLa cells from E2-mediated growth arrest. (asm.org)
  • Our data indicate that the pathway of E2-mediated growth arrest of HeLa cells requires repression of E6 and E7 expression through an activity specified by the transactivation domain of E2TA. (asm.org)
  • An expanded analysis of TP53 polymorphisms may add insight because other SNPs in addition to that at codon 72 might also affect protein expression. (aacrjournals.org)
  • IL‑12 and interferon‑γ serum levels were measured and immunization with LL‑E7P‑IL‑12D was shown to induce an E7‑specific immune response and to confer protection against TC‑1‑induced tumors in vivo. (spandidos-publications.com)
  • The patients were vaccinated with a single dose of TA-HPV and kept in strict isolation to monitor local and systemic side-effects, environmental spread, and anti-E6/E7 immune responses. (openrepository.com)
  • RATIONALE: Vaccines made from human papillomavirus may help the body build an effective immune response to kill HIV cells. (bioportfolio.com)
  • Critical ingredients are genotypes of the human papillomavirus (HPV) and the patient's immune resistance. (bmj.com)
  • The primary regulators of apoptosis are proteins belonging to a group known as the Bcl-2 family. (scientificamerican.com)
  • Bcl-2, Bcl-XL, Bcl-W, Mcl-1 and A1 proteins function as anti-apoptotic proteins that inhibit apoptosis, while Bax, Bad, Bid, Bok, Bik and Bak (I swear these names are not made up! (scientificamerican.com)
  • are pro-apoptotic proteins that trigger apoptosis when activated. (scientificamerican.com)
  • Vaccines or candidate vaccines used to prevent PAPILLOMAVIRUS INFECTIONS. (bioportfolio.com)
  • A vaccine containing L1 capsid proteins from four types of HPV (ALPHAPAPILLOMAVIRUS), types 6, 11, 16 and 18 that is used to prevent infections from HUMAN PAPILLOMAVIRUSES of these subtypes. (bioportfolio.com)
  • This mouse papillomavirus provides, for the first time, the opportunity to study papillomavirus infections in the context of a small common laboratory animal for which abundant reagents are available and for which many strains exist. (mdpi.com)
  • In the few years since it was first reported, MmuPV1 has become a valuable animal papillomavirus because it provides, for the first time, the opportunity to study papillomavirus infections in the context of a small common laboratory animal for which abundant reagents are available and for which many strains exist. (mdpi.com)
  • To localize biochemical activities associated with E7, we chemically synthesized the full-length 98-amino-acid polypeptide and several deletion mutant peptides. (asm.org)
  • This detailed motif has been used to map potential epitopes from SIVmac239 regulatory proteins and to engineer peptides with increased binding capacity. (jimmunol.org)
  • Here, we show that bovine papillomavirus (BPV) E2-induced growth arrest of HeLa cells requires the repression of the E6 and E7 promoter. (asm.org)
  • He first studied a bovine papillomavirus, then focused on the E5, E6, and E7 proteins of the human papillomavirus. (wikipedia.org)
  • The E1 protein of bovine papilloma virus 1 is an ATP-dependent DNA helicase. (springer.com)
  • In this study, we used a murine E7-expressing tumor model (TC1 cells) to assess effects of an E7-based vaccine on tumor growth. (aacrjournals.org)
  • E7 associates with p27KIP1 both in a reconstituted in vitro system and in extracts of mammalian cells, and association requires the C-terminal part of E7. (nih.gov)
  • Two HPV proteins, E6 and E7, are consistently expressed in tumour cells. (openrepository.com)
  • Peripheral blood mononuclear cells were stimulated with HPV-16 E6 or E7 fusion protein. (asm.org)
  • Cells from an autologous B-lymphoblastoid cell line, infected with vaccinia virus expressing E6 or E7, served as target cells. (asm.org)
  • Detection of human mammary tumour virus proteins in human breast cancer cells. (springer.com)
  • Here we have identified shank-interacting protein-like 1 (SIPL1) as a PTEN-NR in human tumor cell lines and human primary cervical cancer cells. (jci.org)
  • Affilin molecules selected against the human papillomavirus E7 protein inhibit the proliferation of target cells. (uni-halle.de)
  • Protein complexes between Rb and MCM7 were also detected in anti-Rb immunoprecipitates prepared from human cells. (asm.org)
  • Once the DNA plasmids enter muscle cells, they instruct those cells to produce the E6 and E7 proteins as well as IL-12. (uchospitals.edu)
  • It is present on the viral membrane and functions as a host receptor binding protein mediating entry into host cells. (sbwire.com)
  • Extensive studies on HPV E6 and E7 proteins have demonstrated their involvement in malignant transformation. (nih.gov)
  • The HPV-16 E6 and E7 proteins are constitutively produced in cervical carcinomas, and E7 was shown to interact with several cell compounds, causing deregulation of the cell cycle and cell transformation ( 43 ). (asm.org)
  • DiMaio D, Mattoon D. Mechanisms of cell transformation by papillomavirus E5 proteins. (springer.com)
  • Molecular mechanisms of transformation by the human papillomaviruses. (springer.com)
  • The c lustered r egularly i nterspaced s hort p alindromic r epeats/CRISPR associated protein 9 (CRISPR/ Cas9) system is a versatile technology that provides the ability to add or remove DNA in the genome in a sequence-specific manner. (nature.com)
  • To illustrate the applicability of the method, we have chosen two viral datasets, namely papillomaviruses and Turnip mosaic virus (TuMV) isolates, largely dissimilar in genome, evolutionary distance and biology. (biomedcentral.com)
  • These results suggest that E6 and E7 proteins bind to the tumor suppressor counterparts, thereby preventing transport of these proteins into the nucleus. (bvsalud.org)
  • The anti-apoptotic proteins bind to and inactivate the pro-apoptotic proteins in a healthy cell that does not need to die. (scientificamerican.com)
  • In response to pro-apoptotic signals (from pro-apoptotic proteins such as Bax), cytochrome c is released into the cell by the mitochondria, and they bind to a protein known as Apaf-1. (scientificamerican.com)
  • Full-length Rb and MCM7 form protein complexes in vitro, and the amino termini of two Rb-related proteins, p107 and p130, also bind MCM7. (asm.org)
  • It is therefore a good candidate for heterologous protein secretion in different applications ranging from industrial production of high-added-value proteins to in vivo use as a live vaccine. (asm.org)
  • Conclusions: The encouraging results obtained from this study performed on a limited number of subjects justify further analysis of the efficacy of the PD-E7/AS02B vaccine in CIN patients. (ac.be)
  • Human papillomavirus vaccine. (isciii.es)
  • Gardasil (Merck, Whitehouse Station, NJ, USA), a quadrivalent HPV VLP prophylactic vaccine containing the L1 proteins of HPV-16, -18, -6, and -11, was the first to be approved by the U.S. Food and Drug Administration (FDA), in 2006. (asm.org)
  • Rietscher K, Keil R, Jordan A, Hatzfeld M. 14-3-3 proteins regulate desmosomal adhesion via plakophilins. (uni-halle.de)
  • SUMOylation of viral proteins is widespread and serves to modify or regulate the properties of those proteins. (portlandpress.com)
  • The gram-positive and generally regarded as safe (GRAS) commensal bacterium Streptococcus gordonii was used for this purpose to display E7 protein at the cell surface in fusion with export signals ( 30 ). (asm.org)
  • Enhanced Cytotoxic CD8 T Cell Priming Using Dendritic Cell-Expressing Human Papillomavirus-16 E6/E7-p16INK4 Fusion Protein with Sequenced Anti-Programmed Death-1. (sigmaaldrich.com)
  • Five vaccinated patients (71%) showed significantly increased IFN-gamma CD8(+) cell responses upon PD-E7 stimulation. (ac.be)
  • Among polarity proteins, we focused on human disc large (DLG1), which is localized mainly at adherens junctions and contributes to the control of cell proliferation. (microbiologyresearch.org)
  • With respect to the transforming activity of these proteins , many investigators have reported the location of these proteins in the cell , but their results are still controversial. (bvsalud.org)
  • The objective of this study was to characterize the phenotype(s) of the effector cell population responsible for HPV-16 E6- and E7-specific cytotoxic responses. (asm.org)
  • p0071/PKP4, a multifunctional protein coordinating cell adhesion with cytoskeletal organization. (uni-halle.de)
  • Although mutations of p107 or p130 have not yet been associated with human neoplasia, evidence from nullizygous animals indicates that these Rb-related proteins play an important supportive role in the regulation of cell proliferation and differentiation ( 12 , 50 ). (asm.org)
  • However, substantial cell replication occurs in Drosophila melanogaster embryos with mutations in E2F or DP-1 after apparent exhaustion of the maternally derived proteins ( 88 ). (asm.org)
  • Alteration of these cell cycle-related proteins could be reversed by ectopic Eps8, implicating that the effect of Eps8 on the mentioned cell cycle modulators was specific. (aacrjournals.org)
  • E7 promotes cell proliferation by associating with the tumor suppressor pRb and other cell-cycle-regulatory proteins ( 4 ), and E6 binds to and inactivates p53 tumor suppressor protein and several proteins that participate in cell-cell adhesion ( 5 ). (aacrjournals.org)
  • p53 functions as a cell cycle control protein in osteosarcomas. (pubmedcentralcanada.ca)
  • The papillomavirus life cycle is intimately connected with the differentiation process of stratified epithelium, and several viral early proteins function to modulate the host cell environment. (portlandpress.com)
  • Many different papillomaviruses infect animals, and over 150 genotypes have been so far identified in humans. (hindawi.com)
  • Mori S, Takeuchi T, Ishii Y, Yugawa T, Kiyono T, Nishina H, and Kukimoto I: Human Papillomavirus 16 E6 Upregulates APOBEC3B via the TEAD Transcription Factor. (go.jp)
  • One of the critical early proteins is the E2 protein, which functions in both viral replication and transcription. (portlandpress.com)
  • Global Markets Direct's, 'Human Papilloma Virus (HPV) E7 Protein - Pipeline Review, H1 2016', provides in depth analysis on Human Papilloma Virus (HPV) E7 Protein targeted pipeline therapeutics. (reportsnreports.com)
  • The papillomavirus E2 proteins play essential roles in transcriptional regulation and viral DNA replication. (asm.org)
  • The conserved N-terminal 200-amino-acid domain is required for the transcriptional activation function, the DNA replication function, and the association with replication protein E1. (asm.org)
  • Two independent cDNAs resulting from this screen were found to encode the carboxy-terminal 137 amino acids of MCM7, a member of a family of proteins that comprise replication licensing factor. (asm.org)