Amylases: A group of amylolytic enzymes that cleave starch, glycogen, and related alpha-1,4-glucans. (Stedman, 25th ed) EC 3.2.1.-.Pancreas: A nodular organ in the ABDOMEN that contains a mixture of ENDOCRINE GLANDS and EXOCRINE GLANDS. The small endocrine portion consists of the ISLETS OF LANGERHANS secreting a number of hormones into the blood stream. The large exocrine portion (EXOCRINE PANCREAS) is a compound acinar gland that secretes several digestive enzymes into the pancreatic ductal system that empties into the DUODENUM.Isoamylase: An enzyme that hydrolyzes 1,6-alpha-glucosidic branch linkages in glycogen, amylopectin, and their beta-limit dextrins. It is distinguished from pullulanase (EC 3.2.1.41) by its inability to attack pullulan and by the feeble action of alpha-limit dextrins. It is distinguished from amylopectin 6-glucanohydrolase (EC 3.2.1.69) by its action on glycogen. With EC 3.2.1.69, it produces the activity called "debranching enzyme". EC 3.2.1.68.Saliva: The clear, viscous fluid secreted by the SALIVARY GLANDS and mucous glands of the mouth. It contains MUCINS, water, organic salts, and ptylin.Origanum: A plant genus of the family LAMIACEAE that is the source of a familiar food seasoning.alpha-Amylases: Enzymes that catalyze the endohydrolysis of 1,4-alpha-glycosidic linkages in STARCH; GLYCOGEN; and related POLYSACCHARIDES and OLIGOSACCHARIDES containing 3 or more 1,4-alpha-linked D-glucose units.Ceruletide: A specific decapeptide obtained from the skin of Hila caerulea, an Australian amphibian. Caerulein is similar in action and composition to CHOLECYSTOKININ. It stimulates gastric, biliary, and pancreatic secretion; and certain smooth muscle. It is used in paralytic ileus and as diagnostic aid in pancreatic malfunction.Bethanechol CompoundsCholecystokinin: A peptide, of about 33 amino acids, secreted by the upper INTESTINAL MUCOSA and also found in the central nervous system. It causes gallbladder contraction, release of pancreatic exocrine (or digestive) enzymes, and affects other gastrointestinal functions. Cholecystokinin may be the mediator of satiety.ChymotrypsinogenPancreatitis: INFLAMMATION of the PANCREAS. Pancreatitis is classified as acute unless there are computed tomographic or endoscopic retrograde cholangiopancreatographic findings of CHRONIC PANCREATITIS (International Symposium on Acute Pancreatitis, Atlanta, 1992). The two most common forms of acute pancreatitis are ALCOHOLIC PANCREATITIS and gallstone pancreatitis.Depsides: Phenolic benzoic acid esters.Starch: Any of a group of polysaccharides of the general formula (C6-H10-O5)n, composed of a long-chain polymer of glucose in the form of amylose and amylopectin. It is the chief storage form of energy reserve (carbohydrates) in plants.Pancreas, Exocrine: The major component (about 80%) of the PANCREAS composed of acinar functional units of tubular and spherical cells. The acinar cells synthesize and secrete several digestive enzymes such as TRYPSINOGEN; LIPASE; AMYLASE; and RIBONUCLEASE. Secretion from the exocrine pancreas drains into the pancreatic ductal system and empties into the DUODENUM.Parotid Gland: The largest of the three pairs of SALIVARY GLANDS. They lie on the sides of the FACE immediately below and in front of the EAR.Sincalide: An octapeptide hormone present in the intestine and brain. When secreted from the gastric mucosa, it stimulates the release of bile from the gallbladder and digestive enzymes from the pancreas.Lipase: An enzyme of the hydrolase class that catalyzes the reaction of triacylglycerol and water to yield diacylglycerol and a fatty acid anion. It is produced by glands on the tongue and by the pancreas and initiates the digestion of dietary fats. (From Dorland, 27th ed) EC 3.1.1.3.Isoenzymes: Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.Receptors, Cholecystokinin: Cell surface proteins that bind cholecystokinin (CCK) with high affinity and trigger intracellular changes influencing the behavior of cells. Cholecystokinin receptors are activated by GASTRIN as well as by CCK-4; CCK-8; and CCK-33. Activation of these receptors evokes secretion of AMYLASE by pancreatic acinar cells, acid and PEPSIN by stomach mucosal cells, and contraction of the PYLORUS and GALLBLADDER. The role of the widespread CCK receptors in the central nervous system is not well understood.Electrophoresis, Agar Gel: Electrophoresis in which agar or agarose gel is used as the diffusion medium.Insulin: A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1).Mice, Inbred Strains: Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.MedlinePlus: NATIONAL LIBRARY OF MEDICINE service for health professionals and consumers. It links extensive information from the National Institutes of Health and other reviewed sources of information on specific diseases and conditions.Salivary alpha-Amylases: A subclass of alpha-amylase ISOENZYMES that are secreted into SALIVA.Glucagon-Secreting Cells: A type of pancreatic cell representing about 5-20% of the islet cells. Alpha cells secrete GLUCAGON.Sialorrhea: Increased salivary flow.Glucagon: A 29-amino acid pancreatic peptide derived from proglucagon which is also the precursor of intestinal GLUCAGON-LIKE PEPTIDES. Glucagon is secreted by PANCREATIC ALPHA CELLS and plays an important role in regulation of BLOOD GLUCOSE concentration, ketone metabolism, and several other biochemical and physiological processes. (From Gilman et al., Goodman and Gilman's The Pharmacological Basis of Therapeutics, 9th ed, p1511)Goats: Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.PeroxidasesSodium Hydroxide: A highly caustic substance that is used to neutralize acids and make sodium salts. (From Merck Index, 11th ed)Xylenes: A family of isomeric, colorless aromatic hydrocarbon liquids, that contain the general formula C6H4(CH3)2. They are produced by the destructive distillation of coal or by the catalytic reforming of petroleum naphthenic fractions. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Microtomy: The technique of using a microtome to cut thin or ultrathin sections of tissues embedded in a supporting substance. The microtome is an instrument that hold a steel, glass or diamond knife in clamps at an angle to the blocks of prepared tissues, which it cuts in sections of equal thickness.alpha-Cyclodextrins: Cyclic GLUCANS consisting of six (6) glucopyranose units linked by 1,4-glycosidic bonds.Pancreatic alpha-Amylases: A subclass of alpha-amylase ISOENZYMES that are secreted into PANCREATIC JUICE.Cyclodextrins: A homologous group of cyclic GLUCANS consisting of alpha-1,4 bound glucose units obtained by the action of cyclodextrin glucanotransferase on starch or similar substrates. The enzyme is produced by certain species of Bacillus. Cyclodextrins form inclusion complexes with a wide variety of substances.Crystallization: The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Crystallography, X-Ray: The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Acarbose: An inhibitor of ALPHA-GLUCOSIDASES that retards the digestion and absorption of DIETARY CARBOHYDRATES in the SMALL INTESTINE.Pancreatic Diseases: Pathological processes of the PANCREAS.Pancreatectomy: Surgical removal of the pancreas. (Dorland, 28th ed)Electrophoresis: An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.Carbohydrate Metabolism: Cellular processes in biosynthesis (anabolism) and degradation (catabolism) of CARBOHYDRATES.Laboratories: Facilities equipped to carry out investigative procedures.Trypsinogen: The inactive proenzyme of trypsin secreted by the pancreas, activated in the duodenum via cleavage by enteropeptidase. (Stedman, 25th ed)Trypsin: A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.Malabsorption Syndromes: General term for a group of MALNUTRITION syndromes caused by failure of normal INTESTINAL ABSORPTION of nutrients.Calcium: A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.Carbachol: A slowly hydrolyzed CHOLINERGIC AGONIST that acts at both MUSCARINIC RECEPTORS and NICOTINIC RECEPTORS.Cytosol: Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.Quinuclidinyl Benzilate: A high-affinity muscarinic antagonist commonly used as a tool in animal and tissue studies.Receptors, Muscarinic: One of the two major classes of cholinergic receptors. Muscarinic receptors were originally defined by their preference for MUSCARINE over NICOTINE. There are several subtypes (usually M1, M2, M3....) that are characterized by their cellular actions, pharmacology, and molecular biology.QuinuclidinesMice, Inbred C57BLMuscarinic Antagonists: Drugs that bind to but do not activate MUSCARINIC RECEPTORS, thereby blocking the actions of endogenous ACETYLCHOLINE or exogenous agonists. Muscarinic antagonists have widespread effects including actions on the iris and ciliary muscle of the eye, the heart and blood vessels, secretions of the respiratory tract, GI system, and salivary glands, GI motility, urinary bladder tone, and the central nervous system.Mice, Knockout: Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
"Entrez Gene: AMY2B amylase, alpha 2B (pancreatic)". Human AMY2B genome location and AMY2B gene details page in the UCSC Genome ... 1988). "Human pancreatic amylase is encoded by two different genes". Nucleic Acids Res. 16 (10): 4724. doi:10.1093/nar/16.10. ... Alpha-amylase 2B is an enzyme that in humans is encoded by the AMY2B gene. Amylases are secreted proteins that hydrolyze 1,4- ... 2001). "alpha-Amylase expressed in human liver is encoded by the AMY-2B gene identified in tumorous tissues". Clin. Chim. Acta ...
"Specific interactions of pancreatic amylase at acidic pH. Amylase and the major protein of the zymogen granule membrane (GP-2) ... "The pancreatic membrane protein GP-2 localizes specifically to secretory granules and is shed into the pancreatic juice as a ... Pancreatic secretory granule membrane major glycoprotein GP2 is a protein that in humans is encoded by the GP2 gene. GRCh38: ... Parker EM, Zaman MM, Freedman SD (2001). "GP2, a GPI-anchored protein in the apical plasma membrane of the pancreatic acinar ...
Some carbohydrates are degraded into simple sugars, or monosaccharides (e.g., glucose). Pancreatic amylase breaks down some ... Pancreatic lipase breaks down triglycerides into free fatty acids and monoglycerides. Pancreatic lipase works with the help of ... It also receives bile and pancreatic juice through the pancreatic duct, controlled by the sphincter of Oddi. The primary ... Pancreatic enzymes and bile from the gallbladder enter the small intestine in response to the hormone cholecystokinin, which is ...
Acarbose also blocks pancreatic alpha-amylase in addition to inhibiting membrane-bound alpha-glucosidases. Pancreatic alpha- ... Moreover, acarbose inhibits pancreatic alpha-amylase in addition to alpha-glucosidase. There are a large number of natural ... amylase hydrolyzes complex starches to oligosaccharides in the lumen of the small intestine. Inhibition of these enzyme systems ... Alpha-glucosidase Alpha amylase inhibitor Benalla, Wafaa; Bellahcen, Said; Bnouham, Mohamed (2010). "Antidiabetic Medicinal ...
NH2 fragment with potent pancreatic amylase inhibitory activity. Regulatory Peptides, 39:9-17, (1992). 5. Mungan Z, Arimura A, ... Structure-dependent effect on pancreatic amylase secretion and jejunal strip contraction. Eur J Pharmacol, 240:259-267, (1993 ... Development of a potent bombesin receptor antagonist with prolonged in vivo inhibitory activity on bombesin-stimulated amylase ... Primary splenic artery aneurysm ruptured into pancreatic serous cystadenoma. Turk J Gastroenterol 19:57-63 (2008) 40. Mungan Z ...
Buisson, G; Duee, E; Haser, R; Payan, F (December 20, 1987). "Three dimensional structure of porcine pancreatic alpha-amylase ... Glycogen branching enzyme belongs to the α-amylase family of enzymes, which include α-amylases, pullulanas/isoamylase, ... While the central (α/β) barrel domain is common in members of the α-amylase family, numerous variations exist between the ... Through primary structure analysis and the x-ray crystallographic structures of the members of the α-amylase family, seven ...
Smith R, Southwell-Keely J, Chesher D (2005). "Should serum pancreatic lipase replace serum amylase as a biomarker of acute ... Reasons for false positive elevated serum amylase include salivary gland disease (elevated salivary amylase), bowel obstruction ... "Should Serum Pancreatic Lipase Replace Serum Amylase As a Biomarker of Acute Pancreatitis?". ANZ Journal of Surgery. 75 (6): ... pancreatic ascites, pleural effusion, sterile/infected pancreatic necrosis. Systemic complications include ARDS, multiple organ ...
They also secrete enzymes to enhance host digestive enzymes e.g. pancreatic α-amylase. Schistosomes, another type of ...
Pancreatic alpha-amylase is an enzyme that in humans is encoded by the AMY2A gene. Amylases are secreted proteins that ... Jacob M, Lainé J, LeBel D (1993). "Specific interactions of pancreatic amylase at acidic pH. Amylase and the major protein of ... 1994). "The active center of a mammalian alpha-amylase. Structure of the complex of a pancreatic alpha-amylase with a ... 1988). "Primary structure of human pancreatic alpha-amylase gene: its comparison with human salivary alpha-amylase gene". Gene ...
1986). "Pancreatic amylase expression in human pancreatic development". Hybridoma. 5 (2): 137-45. doi:10.1089/hyb.1986.5.137. ... 1988). "Primary structure of human pancreatic alpha-amylase gene: its comparison with human salivary alpha-amylase gene". Gene ... 1994). "Amylase mRNA transcripts in normal tissues and neoplasms: the implication of different expressions of amylase isogenes ... This gene is found in many organisms, see Alpha-Amylase. Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside ...
Produced by the pancreas, it is found in pancreatic juice, along with amylase, lipase, and chymotrypsinogen. It is activated by ... Premature trypsin activation can be destructive and may trigger a series of events that lead to pancreatic self-digestion. In ... These are the cationic, anionic, and meso trypsinogen, and they account for 23.1%, 16%, and 0.5% of total pancreatic secretory ... ISBN 0-471-58651-X. Scheele G, Bartelt D, Bieger W (March 1981). "Characterization of human exocrine pancreatic proteins by two ...
2007). "A cytosolic splice variant of Cab45 interacts with Munc18b and impacts on amylase secretion by pancreatic acini". Mol. ...
Amylase levels are elevated in cases of esophageal rupture, pancreatic pleural effusion, or cancer. Glucose is decreased with ... and amylase, glucose, pH and cell counts should be measured. Red blood cell counts are elevated in cases of bloody effusions ( ...
This will be acted on slowly by pancreatic amylase and glucose will be absorbed over a 6 hour period. The overall frequency of ...
"Influence of Some Organic Compounds upon the Hydrolysis of Starch by Salivary and Pancreatic Amylases." Naylor taught public ... "Influence of Some Organic Compounds upon the Hydrolysis of Starch by Salivary and Pancreatic Amylases" (Columbia University ... "Influence of Some Organic Compounds upon the Hydrolysis of Starch by Salivary and Pancreatic Amylases" (Columbia University ...
During digestion, bonds between glucose molecules are broken by salivary and pancreatic amylase, resulting in progressively ... Salivary amylase is contained in saliva and starts the breakdown of carbohydrates into monosaccharides. Most digestive enzymes ... In the small intestine, the larger part of digestion takes place and this is helped by the secretions of bile, pancreatic juice ... Saliva, a liquid secreted by the salivary glands, contains salivary amylase, an enzyme which starts the digestion of starch in ...
Other indicators include raised indicators of ampulla of vater (pancreatic duct obstruction) such as lipases and amylases. In ...
She attempted to find a more pure form of amylase and she was able to develop a method for isolating crystalline pancreatic ... most notably finding a method for purifying crystalline porcine pancreatic amylase. She spent sixty years doing this. Caldwell ... During her time as a chemist, Caldwell did research on a type of enzyme called amylase. She spent a lot of her time trying to ... In 1960, Caldwell was awarded the Garvin Medal by the American Chemistry Society, for her research in amylase. The Garvin Medal ...
"Retroviral and pseudogene insertion sites reveal the lineage of human salivary and pancreatic amylase genes from a single gene ...
"Retroviral and pseudogene insertion sites reveal the lineage of human salivary and pancreatic amylase genes from a single gene ... and one type of amylase is encoded by the alpha-amylase gene (AMY1). The AMY1 locus, as well as the amylase enzyme, is one of ... One example of a whole gene repeat is the alpha-amylase 1 gene (AMY1) that encodes alpha-amylase which has a significant copy ... Currently it is unknown which mechanism gave rise to the initial duplication of the amylase gene, and it can imply that the ...
... pancreatic amylase and pancreatic lipase). It can be used on the abdominal aorta to detect or exclude abdominal aortic aneurysm ... Ultrasound can be used for additional anatomical information for patients with an abnormal kidney function or pancreatic ...
Pancreatic amylase that breaks down starch and glycogen which are alpha-linked glucose polymers. Humans lack the cellulases to ... Salivary amylase: Carbohydrate digestion also initiates in the mouth. Amylase, produced by the salivary glands, breaks complex ... CCK stimulates production of the pancreatic zymogens.. Pancreatic juice, composed of the secretions of both ductal and acinar ... The following significant pancreatic bio-feedback mechanisms are essential to the maintenance of pancreatic juice balance/ ...
... and pancreatic alpha-amylase. Pancreatic alpha-amylase hydrolyzes complex starches to oligosaccharides in the lumen of the ...
... pancreatic amylase and pancreatic lipase) to function optimally. Secretin targets the pancreas; pancreatic centroacinar cells ... Secretin is used in a diagnostic tests for pancreatic function; secretin is injected and the pancreatic output can then be ... It also stimulates bile production by the liver; the bile emulsifies dietary fats in the duodenum so that pancreatic lipase can ... Villanger O, Veel T, Raeder MG (1995). "Secretin causes H+/HCO3- secretion from pig pancreatic ductules by vacuolar-type H(+)- ...
One isomer of HCA, known as (2S,3R)-HCA, inhibits pancreatic alpha-amylase and intestinal alpha-glucosidase, leading to a ...
α-amylase (EC3.2.1.1), or ptyalin, secreted by the acinar cells of the parotid and submandibular glands, starts the digestion ... Salivary lipase plays a large role in fat digestion in newborn infants as their pancreatic lipase still needs some time to ... Saliva contains the enzyme amylase, also called ptyalin, which is capable of breaking down starch into simpler sugars such as ... such as amylase and lipase), antimicrobial agents such as secretory IgA and lysozyme.[1] The enzymes found in saliva are ...
... amylase: …by the salivary glands, whereas pancreatic amylase is secreted by the pancreas into the small intestine. ... In amylase. …by the salivary glands, whereas pancreatic amylase is secreted by the pancreas into the small intestine. ... Other articles where Pancreatic amylase is discussed: ... Pancreatic amylase. biochemistry. THIS IS A DIRECTORY PAGE. ...
Amylase enters the bloodstream when the pancreas becomes inflamed... ... The pancreas and the glands that produce saliva also produce amylase, an enzyme that helps the body digest food, notes ... Is an elevation of pancreatic enzymes dangerous?. A: Elevated pancreatic enzymes such as amylase and lipase can indicate ... What does the amylase test indicate for the pancreas?. A: The amylase test indicates whether the pancreas is damaged or blocked ...
Pharmacological Actions : Alpha-amylase inhibitor, Alpha-glucosidase inhibitor , Enzyme Inhibitors: Pancreatic Amylase ... Pharmacological Actions : Enzyme Inhibitors: Pancreatic Amylase, Enzyme Inhibitors: Pancreatic Lipase, Pancreato Protective ... Pharmacological Actions : Enzyme Inhibitors: Pancreatic Amylase, Enzyme Inhibitors: Pancreatic Lipase, Pancreato Protective ... 6 Abstracts with Enzyme Inhibitors: Pancreatic Amylase Research. Filter by Study Type. Animal Study. ...
Buy our Natural human Pancreatic alpha amylase protein. Ab77861 is an active full length protein produced in Nativesyntheticaly ... Natural human Pancreatic alpha amylase protein. See all Pancreatic alpha amylase proteins and peptides. ... from a total amylase concentration in serum of about 120µg/L. Pancreatic amylase and salivary amylase are the products of ... Pancreatic amylase is a digestive enzyme secreted by the pancreas. It consists of a single polypeptide chain with a molecular ...
Browse our Pancreatic Amylase Beta RNAi catalog backed by our Guarantee+. ... Pancreatic Amylase Beta RNAi available through Novus Biologicals. ... Alternate Names for Pancreatic Amylase Beta RNAi. Pancreatic Amylase Beta RNAi, AMY2B RNAi, alpha-amylase 2B RNAi, AMY2 RNAi, ... Pancreatic Amylase Beta RNAi. We offer Pancreatic Amylase Beta RNAi for use in common research applications. Each Pancreatic ...
... products and learn more about anti-Pancreatic Amylase Alpha, Clone: RPA-B5, Novus Biologicals 0.1mg; 0.1mg; Unlabeled. ... Pancreatic Amylase Alpha Monoclonal antibody specifically detects Pancreatic Amylase Alpha in Rat samples. It is validated for ... AMY2, AMY2A amylase, alpha 2A (pancreatic), AMY2B, PA, pancreatic alpha-amylase. Host Species. Mouse. ...
Carbohydrate and protein-based inhibitors of porcine pancreatic alpha-amylase: structure analysis and comparison of their ... PIG PANCREATIC ALPHA-AMYLASE COMPLEXED WITH THE OLIGOSACCHARIDE V-1532. *DOI: 10.2210/pdb1pig/pdb ... ALPHA-AMYLASE A 496 Sus scrofa EC#: 3.2.1.1 IUBMB Mutation: I49V, Q243S, A310S, E404Q, D451N, E484Q Gene Name(s): AMY2 ...
Pancreatic Amylase , , Does anyone out there have a pancreatic amylase that works on paraffin , embedded tissue? Weve tried ... RE: Pancreatic Amylase. ,, Previous Message , Next Message ,, From:. Hewlett Bryan (CMH) ,[email protected],. ... Fran, We use the monoclonal anti- Pancreatic Amylase Cat# MU099-UC from Biogenex. Formaldehyde-fixed(16-24hrs) paraffin- ...
... products and learn more about anti-Pancreatic Amylase Alpha, Clone: 6D4, Novus Biologicals 0.1mL; Unlabeled:Life 0.1mL; ... Pancreatic Amylase Alpha Monoclonal antibody specifically detects Pancreatic Amylase Alpha in Human samples. It is validated ... This antibody is specific for Homo sapiens amylase, alpha 2A (pancreatic) (AMY2A).. ... AMY2, AMY2A amylase, alpha 2A (pancreatic), AMY2B, PA, pancreatic alpha-amylase. Host Species. Mouse. ...
X-ray crystallographic analyses of pig pancreatic alpha-amylase with limit dextrin, oligosaccharide, and alpha-cyclodextrin. ... Pancreatic alpha-amylase A 496 Sus scrofa EC#: 3.2.1.1 IUBMB Fragment: residues 16-511 Mutation: Q1X, A411D Gene Name(s): AMY2 ... X-ray Crystallographic Analysis of Pig Pancreatic Alpha-Amylase with Alpha-cyclodextrin. *DOI: 10.2210/pdb3l2m/pdb ...
... amylase, alpha 2B (pancreatic)) for ELISA, WB. Anti-Pancreatic Amylase pAb (GTX39406) is tested in Human, Pig samples. 100% Ab- ... AMY2 Antibody , AMY2B Antibody , amylase, alpha 2B (pancreatic) Antibody. Specificity. This antibody recognizes Amylase ( ... Western Blot (WB) : This antibody detects a major band of approximately 56 kDa in porcine amylase samples under reducing ... Pancreatic), a digestive enzyme with a molecular weight of approximately 56 kDa secreted by the pancreas. ...
Davidson T, Yaghoobi M, Davidson BR, Gurusamy K. Amylase in drain fluid for the diagnosis of pancreatic leak in post-pancreatic ... Drain use after pancreatic surgery. *Blood tests for the diagnosis of pancreatic necrosis (pancreatic destruction due to ... Amylase in drain fluid for the diagnosis of pancreatic leak after partial removal of the pancreas. Background ... To determine the diagnostic accuracy of amylase in drain fluid at 48 hours or more for the diagnosis of pancreatic leak in ...
Serum Iso-amylases: An Electrophoretic Study of the Blood Amylase and the Patterns Observed in Pancreatic Disease DAVID A. ... Serum Iso-amylases: An Electrophoretic Study of the Blood Amylase and the Patterns Observed in Pancreatic Disease. Ann Intern ... Amylase in Diagnosis of Pancreatic Disease Annals of Internal Medicine; 88 (6): 838-839 ... normal blood amylase is controlled in part at extrapancreatic sites, [2] blood amylase is lowered in states of increased ...
Essential Therapeutics Digestive Enzymes is a high potency pancreatic supplement that breaks down fats, proteins, and ... Composed of amylase, lipase, and protease, pancreatin is traditionally used to supplement the body s own pancreatic secretions ... Essential Therapeutics Digestive Enzymes are a high potency pancreatic supplement that contains Protease, Amylase and Lipase to ... Amylase 60,000 USP Units-Breaks down starch "-1,4 glucan bonds". Breaks down carbohydrates, starches, and sugars that are ...
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Caerulein and carbamoylcholine stimulate pancreatic amylase release at resting cytosolic free Ca2+. R Bruzzone, T Pozzan, C B ... Cytosolic free calcium concentrations ([Ca2+]i) and amylase secretion were measured in isolated rat pancreatic acini loaded ... Caerulein and carbamoylcholine stimulate pancreatic amylase release at resting cytosolic free Ca2+ ... Caerulein and carbamoylcholine stimulate pancreatic amylase release at resting cytosolic free Ca2+ ...
... K-REx Repository. Search K-REx. This Community. ... Pancreatic alpha-amylase activity was 54% higher in cattle fed at twice maintenance energy than in cattle fed at maintenance ... Dietary influences on pancreatic amylase and small intestinal disaccharidase activities in cattle ...
However, another pancreatic secretagogue, bombesin, retained its ability to fully stimulate amylase secretion in acinar ... Cholinergic Stimulation of Amylase Secretion from Pancreatic Acinar Cells Studied with Muscarinic Acetylcholine Receptor Mutant ... Cholinergic Stimulation of Amylase Secretion from Pancreatic Acinar Cells Studied with Muscarinic Acetylcholine Receptor Mutant ... Cholinergic Stimulation of Amylase Secretion from Pancreatic Acinar Cells Studied with Muscarinic Acetylcholine Receptor Mutant ...
Download PDF Pancreatic cystic lesion with baffling fluid levels of cea and amylase. Item availability may be restricted. ... Pancreatic cystic lesion with baffling fluid levels of cea and amylase. Journal of Gastrointestinal and Liver Diseases, 25(3): ...
Comparison with pancreatic alpha-amylase. - M K Reddy, G D Heda, J K Reddy ... Purification and characterization of alpha-amylase from rat pancreatic acinar carcinoma. ... Comparison with pancreatic alpha-amylase.. Abstract. alpha-Amylase was purified to apparent homogeneity from normal pancreas ... Antibodies raised against the purified pancreatic alpha-amylase and tumour alpha-amylase respectively showed only one positive ...
Derivatives of maltose and maltotriose were chemically synthesised as substrates for human pancreatic alpha-amylases and ... Synthesis and characterisation of novel chromogenic substrates for human pancreatic alpha-amylase. ... Synthesis and characterisation of novel chromogenic substrates for human pancreatic alpha-amylase. ... Synthesis and characterisation of novel chromogenic substrates for human pancreatic alpha-amylase ...
Pancreatic Amylase antibody LS-C700680 is a Cy3-conjugated rabbit polyclonal antibody to Pancreatic Amylase (AMY2A ) from human ... Pancreatic Amylase antibody LS-C700680 is a Cy3-conjugated rabbit polyclonal antibody to Pancreatic Amylase (AMY2A ) from human ... AMY2A / Pancreatic Amylase Antibody (aa22‑257, Cy3) LS‑C700680 AMY2A / Pancreatic Amylase Antibody (aa22‑257, Cy3) LS‑C700680 ...
Structural and mechanistic studies of chloride induced activation of human pancreatic alpha-amylase. ... Structural and mechanistic studies of chloride induced activation of human pancreatic alpha-amylase. ... Structural and mechanistic studies of chloride induced activation of human pancreatic alpha-amylase ... through structural and kinetic experiments focusing on the chloride-dependent N298S variant of human pancreatic alpha-amylase ( ...
Meta-analysis of drain amylase content on postoperative day 1 as a predictor of pancreatic fistula following pancreatic ... Drain amylase content in the days immediately after major pancreatic resection has been investigated previously as a predictor ... The purpose of this study was to evaluate the accuracy of drain amylase content on the first day after major pancreatic ... Evaluation of drain amylase content on the first day after surgery is highly accurate in predicting POPF following major ...
We aimed to re-assess the relationship between amylase and adiposity using a systems biology approach. We assessed the ... These findings provide new insights into the involvement of amylase in adiposity and starch metabolism. ... amylases hydrolyze starch. Copy number of AMY1A (encoding AMY1) was reported to be higher in populations with a high-starch ... AMY1 salivary amylase, AMY2 pancreatic amylase, Chr chromosome, MAF minor allele frequency, SE standard error ...
LipaseEnzymeEnzymesStarchAMY2AAcinarAMY2BSalivaSecreted by the pancreasDigestionSmall intestineAbstractAntibodyExocrineAlpha 2ACarbohydratesPorcine pancreatic alpha-amylaseAciniInhibitorsMaltaseAMY1IntestinalEliciting amylase secretionSecretionsTotal amylaseSerum amylasePancreatitisVitroDiagnosis of Pancreatic DiseaseCatalyzes the hydrolysisInhibitionDuctSpecificityGenesPancreas and salivary glandsInhibitor of humanAcuteAminoGlucoseProteinsSalivary amylase geneSecretion was significantlyUrineTypes of amylaseStimulation of amylaseMaltoseELISABlood amylase levelSpeciesProteinProteaseDiagnose pancreaticHuman
- Elevated pancreatic enzymes such as amylase and lipase can indicate pancreatitis, a serious inflammation of the pancreas that causes these enzymes to be si. (reference.com)
- Essential Therapeutics Digestive Enzymes are a high potency pancreatic supplement that contains Protease, Amylase and Lipase to prevent malabsorption and gastric discomfort. (advancedalternativescenter.com)
- Composed of amylase, lipase, and protease, pancreatin is traditionally used to supplement the body s own pancreatic secretions. (advancedalternativescenter.com)
- In vitro α-amylase and pancreatic lipase inhibitory activity of Cornus" by A. Świerczewska, T. Buchholz et al. (jfda-online.com)
- These lipases, unlike alkaline lipases (such as pancreatic lipase ), do not require bile acid or colipase for optimal enzymatic activity. (wikipedia.org)
- Blood amylase and lipase levels are most frequently drawn to diagnose pancreatitis. (chemocare.com)
- This can cause amylase and lipase levels to be increased up to 3 times the normal limit. (chemocare.com)
- Pancreatic triacylglycerol lipase (PL), α-amylase, and α-glucosidase are appealing pharmacological targets for the management of dyslipidemia, atherosclerosis, and obesity-diabetes. (uniss.it)
- BestBets: Serum amylase or lipase to diagnose pancreatitis in patients presenting with abdominal pain. (bestbets.org)
- You wonder whether a serum lipase might be better than serum amylase as a diagnostic marker for pancreatitis in this patient. (bestbets.org)
- exp Abdominal pain OR abdominal pain.mp} OR {exp Pancreatitis OR exp Pancreatitis, Acute Necrotizing OR exp pancreatitis, alcoholic OR pancreatitis.mp} AND {exp Amylases OR amylase.mp} AND {exp Lipase OR lipase.mp} AND exp Diagnosis OR diagnosis.mp] LIMIT to human AND English. (bestbets.org)
- Similar ROC curves for amylase and lipase. (bestbets.org)
- Area under ROC curve similar for serum amylase and lipase. (bestbets.org)
- The studies mentioned suggest that serum amylase and lipase both have high levels of sensitivity and specifictiy for pancreatitis, although few studies looked directly at patients attending the emergency department with abdominal pain. (bestbets.org)
- On the whole comparative studies show serum lipase to be slightly superior to amylase as a diagnostic marker when used to rule in or out pancreatitis. (bestbets.org)
- Serum amylase and lipase are high sensitivity and specific diagnostic markers of acute pancreatitis. (bestbets.org)
- Kazmierczak SC, Van Lente F, Hodges ED. Diagnostic and prognostic utility of phospholipase A activity in patients with acute pancreatitis: comparison with amylase and lipase. (bestbets.org)
- Serum amylase and lipase in the evaluation of acute abdominal pain. (bestbets.org)
- The aim of the present study is to investigate the effect of the extract of the leaves of Moringa stenopetala on α-glucosidase, pancreatic α-amylase, pancreatic lipase, and pancreatic cholesterol esterase activities, and, therefore find out the relevance of the plant in controlling blood sugar and lipid levels. (biomedcentral.com)
- It also slightly inhibited pancreatic α-amylase, pancreatic lipase and pancreatic cholesterol esterase. (biomedcentral.com)
- The result demonstrated the beneficial biochemical effects of Moringa stenopetala by inhibiting intestinal α-glucosidase, pancreatic cholesterol esterase and pancreatic lipase activities. (biomedcentral.com)
- Pancreatic lipase and bile to break down lipids. (mindmeister.com)
- Amylase, lipase and insulin blood test? (healthtap.com)
- If the pancreas is inflamed or damaged, amylase and lipase are often up, and it's not subtle. (healthtap.com)
- Apart from amylase, the pancreas also produces other enzymes that help digest food, such as lipase (to break down fats) and protease (to break down protein). (selfhacked.com)
- Pancreatin contains the pancreatic enzymes lipase, amylase and protease. (netdoctor.co.uk)
- For this reason, the total dose of pancreatic enzyme supplements used in patients with cystic fibrosis should not usually exceed 10 000 units of lipase per kg body-weight daily. (netdoctor.co.uk)
- The pancreas and the glands that produce saliva also produce amylase, an enzyme that helps the body digest food, notes MedlinePlus. (reference.com)
- The enzyme that breaks down starch is amylase. (reference.com)
- Salivary amylase is an enzyme responsible for breaking down starch, according to Dr. Michael J. Gregory, a professor of science at Clinton Community Colleg. (reference.com)
- Pancreatic amylase is a digestive enzyme secreted by the pancreas. (abcam.com)
- This antibody recognizes Amylase (Pancreatic), a digestive enzyme with a molecular weight of approximately 56 kDa secreted by the pancreas. (genetex.com)
- Muscarinic acetylcholine receptors (mAChRs) expressed by pancreatic acinar cells play an important role in mediating acetylcholine-dependent stimulation of digestive enzyme secretion. (aspetjournals.org)
- Pancreatic α-amylase (E.C. 3.2.1.1) is a key enzyme in the digestive system and catalyses the initial step in hydrolysis of starch to a mixture of smaller oligosaccharides consisting of maltose, maltotriose, and a number of α-(l-6) and α-(1 - 4) oligoglucans. (biomedcentral.com)
- Feeding also triggered a depletion of gastric mucosal pepsinogen, a respective 5.7- and 20-fold increase in the peak activities of pancreatic trypsin and amylase, and a respective 2.3- and 5.5-fold increase in the peak activities of intestinal maltase and aminopeptidase-N. Enzyme activities peaked between 2 and 4 days postfeeding and returned to fasting levels by day 10. (biologists.org)
- N. Savion and Z. Selinger, Morphological changes in rat pancreatic slices associated with incubation of enzyme secretion by high concentrations of secretagogues, J. Cell. (springer.com)
- Alpha-amylase 2B is an enzyme that in humans is encoded by the AMY2B gene. (wikipedia.org)
- In this study, we found that there was no significant change of ratio of glycosylated molecules among isozymes produced by the same cultured cells, indicating that glycosylation of amylase is influenced by the type of cell producing the enzyme rather than being an inherent property of the amylase isozymes. (scirp.org)
- Hyperamylasemia may be described as an excess of the pancreatic enzyme - amylase in the blood. (chemocare.com)
- Tumors - Amylase enzyme levels may be increased in some pancreas, salivary, prostate, lung and ovarian tumors. (chemocare.com)
- Medical Dictionary states that salivary amylase is an enzyme produced by the saliva glands to catalyse the hydrolysis, or breaking down, of starch into smaller, simpler compounds. (reference.com)
- Salivary amylase, also referred to as ptyalin, is the name of the enzyme found in saliva. (reference.com)
- This test measures the level of the enzyme amylase in your blood. (ahealthyme.com)
- Amylopectin and α -amylose are broken down by the enzyme amylase. (encyclopedia.com)
- Amylase is an enzyme which helps digest glycogen and starch. (medlineplus.gov)
- Amylase is an enzyme that helped our ancestors adapt to digesting complex carbs. (selfhacked.com)
- High amylase can be due to issues with the salivary glands or the pancreas, which produce this enzyme. (selfhacked.com)
- α-Amylase: an enzyme specificity found in various families of glycoside hydrolases. (ebi.ac.uk)
- Is an elevation of pancreatic enzymes dangerous? (reference.com)
- Alpha amylases are enzymes and, by definition, speed up the rate of reactions. (wisegeek.com)
- We therefore assessed the matched regulation of gastrointestinal performance by comparing the postprandial activities and capacities of gastric (pepsin), pancreatic (amylase and trypsin) and intestinal (aminopeptidase-N and maltase) enzymes, and intestinal nutrient uptake. (biologists.org)
- For the medication, see Pancreatic enzymes (medication) . (wikipedia.org)
- Digestive enzymes of diverse specificities are found in the saliva secreted by the salivary glands , in the secretions of cells lining the stomach, in the pancreatic juice secreted by pancreatic exocrine cells, and in the secretions of cells lining the small and large intestines. (wikipedia.org)
- amylase Any of a group of closely related enzymes that degrade starch, glycogen, and other polysaccharides. (encyclopedia.com)
- Given that these pancreatic juices go to the duodenum, it only makes sense that the duodenum should be sending the message to make the enzymes. (infoplease.com)
- This chemical message is the duodenal hormone cholecystokinin, which causes the release of pancreatic enzymes. (infoplease.com)
- This study sought to asses and characterizes the inhibitory action of methanolic extract of Pithecellobium dulce (P. dulce) seeds on α-amylase and α-glucosidase enzymes as well as to characterize compounds responsible for these activities. (jbiochemtech.com)
- Enzymes such as amylase are very important in digestion since humans cannot directly absorb starch and other large, complex molecules. (reference.com)
- Several enzymes are involved in the breakdown of carbohydrates, including salivary amylase, pancreatic amylase, maltase, sucrase and lactase. (reference.com)
- Your physician can check your stool for pancreatic enzymes. (healthtap.com)
- Aiding digestion of food in conditions where there is reduced or absent production of digestive enzymes by the pancreas, for example in chronic pancreatitis , cystic fibrosis , pancreatic cancer or following surgical removal of the pancreas (pancreatectomy) or stomach (gastrectomy). (netdoctor.co.uk)
- Pancreatin may also be necessary if a tumour (eg pancreatic cancer) obstructs the outflow of enzymes from the pancreas. (netdoctor.co.uk)
- Pancreatic α-amylase inhibitors offer an effective strategy to lower the levels of post-prandial hyperglycemia via control of starch breakdown. (biomedcentral.com)
- The pancreatic form degrades the majority of the starch. (wisegeek.com)
- Amylases split carbohydrates such as starch and sugars into simple sugars such as glucose . (wikipedia.org)
- Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. (wikipedia.org)
- Amylase hydrolyzes starch , glycogen , and dextrin to form in all three instances glucose , maltose , and the limit-dextrins. (encyclopedia.com)
- The amylase of malt digests barley starch to the disaccharides that are attacked by yeast in the fermentation process. (encyclopedia.com)
- Background: Salivary (AMY1) and pancreatic (AMY2) amylases hydrolyze starch. (edu.au)
- Conclusions: These findings provide new insights into the involvement of amylase in adiposity and starch metabolism. (edu.au)
- The starch-digesting amylase that is produced by the pancreas and is present in pancreatic juice. (thefreedictionary.com)
- The digestion of polysaccharide carbohydrates begins in the mouth by action of salivary α-amylase, which catalyzes the hydrolysis of the α-1,4-glycosidic bonds of starch, followed by the action of pancreatic amylase. (diabetesjournals.org)
- We hypothesized that starch digestion-related amylase genetic variants affect the regulation of body adiposity, particularly when the intake of polysaccharide, the substrate for amylase, is changed. (diabetesjournals.org)
- Starch is broken down by salivary amylase. (mindmeister.com)
- Amylases are common in plants, particularly in association with the starch stores of seeds and underground perennating organs such as rhizomes, tubers and taproots. (thefreedictionary.com)
- Interestingly, your amylase genes can tell if you do better on a high- or low-starch diet. (selfhacked.com)
- Studies have linked higher amylase levels to better glucose tolerance after eating starch-rich meals [ 4 , 5 ]. (selfhacked.com)
- One study illustrates this nicely: 7 people with high amylase levels and 7 people with low amylase levels were given a starch-rich meal. (selfhacked.com)
- This means that people with low amylase levels may be at a greater risk of insulin resistance and diabetes on starch-rich diets [ 8 ]. (selfhacked.com)
- This antibody is specific for Homo sapiens amylase, alpha 2A (pancreatic) (AMY2A). (fishersci.com)
- Pancreatic Amylase antibody LS-C700680 is a Cy3-conjugated rabbit polyclonal antibody to Pancreatic Amylase (AMY2A ) from human, mouse and rat. (lsbio.com)
- Three human α-amylases exist: Amy1 (salivary amylase), Amy2A (pancreatic amylase), and Amy2B (expressed in various tissues). (scirp.org)
- AMY1 is located in a gene cluster (1p21.1) that also includes pancreatic amylase genes ( AMY2A and AMY2B ), and AMY1 and AMY2A copy numbers are significantly correlated ( 6 ). (diabetesjournals.org)
- Strikingly, carbachol-induced stimulation of amylase secretion was significantly impaired in acinar preparations from both M 1 and M 3 receptor single KO mice and abolished in acinar preparations from M 1 /M 3 receptor double KO mice. (aspetjournals.org)
- However, another pancreatic secretagogue, bombesin, retained its ability to fully stimulate amylase secretion in acinar preparations from M 1 /M 3 receptor double KO mice. (aspetjournals.org)
- Purification and characterization of alpha-amylase from rat pancreatic acinar carcinoma. (curehunter.com)
- alpha-Amylase was purified to apparent homogeneity from normal pancreas and a transplantable pancreatic acinar carcinoma of the rat by affinity chromatography on alpha-glucohydrolase inhibitor (alpha-GHI) bound to aminohexyl-Sepharose 4B. (curehunter.com)
- The presence of additional isoenzymes in the carcinoma , and dissimilarity of tryptic-digest patterns, may reflect an alteration in gene expression or in the post-translational modification of this protein in this heterogeneously differentiated transplantable pancreatic acinar carcinoma . (curehunter.com)
- Pancreatic amylase is synthesised only in pancreatic tissue by acinar cells. (randox.com)
- R.T. Jensen, G.F. Lemp and J.D. Gardner, Interaction of cholecystokinin with specific membrane receptors on pancreatic acinar cells, Proc. (springer.com)
- J.A. Williams, P. Cray and B. Moffat, Effects of ions on amylase release by dissociated pancreatic acinar cells, J. Biol. (springer.com)
- These data show that GLP-1R is present in pancreatic acinar cells and that GLP-1 can regulate secretion through its receptor and cAMP signaling pathway. (nih.gov)
- In the pancreas, acinar cells secrete the PANCREATIC JUICE . (thefreedictionary.com)
- Animals possess only α-amylases, found in pancreatic juice (as pancreatic amylase ) and also (in humans and some other species) in saliva (as salivary amylase or ptyalin ). (encyclopedia.com)
- Ktihnl P, Tischberger H (1980) Amylase 1 polymorphism of human parotid saliva: detection of a new allele. (springer.com)
- Pronk JC (1977) A genetic variant of amylase from human parotid saliva detected by isoelectric focusing. (springer.com)
- Digestion of your dietary intake of carbohydrates and fat relies on the action of amylase, found in the saliva, to begin the digestion of starches. (chemocare.com)
- Anti-α-amylase antibody reacts specifically with α amylase present in human saliva and pancreatic extract but not with other proteins of saliva and pancreatic extract. (sigmaaldrich.com)
- The antiserum is specific for human a-amylase found in human saliva and human pancreatic extract. (sigmaaldrich.com)
- No reaction with other human saliva proteins or pancreatic extract proteins is observed. (sigmaaldrich.com)
- by the salivary glands, whereas pancreatic amylase is secreted by the pancreas into the small intestine. (britannica.com)
- Alpha amylase is a protein involved in the digestion of food. (wisegeek.com)
- The pancreas also secretes amylase to aid in digestion. (reference.com)
- It lies between the stomach and large intestine , and receives bile and pancreatic juice through the pancreatic duct to aid in digestion . (wikipedia.org)
- One of the successful methods to prevent of the onset of diabetes is to control postprandial hyperglycemia by the inhibition of α-glucosidase and pancreatic α-amylase activities, resulting in the aggressive delay of the carbohydrate digestion of absorbable monosaccharides. (biomedcentral.com)
- In animals, salivary α -amylase begins the digestion process in the mouth. (encyclopedia.com)
- the process is completed in the small intestine by the pancreatic amylase, sometimes called amylopsin. (encyclopedia.com)
- These secretions are exocrine in nature, traveling to the small intestine through the pancreatic duct, which joins up with the common bile duct to form the hepatopancreatic ampulla. (infoplease.com)
- abstract = "Pancreatic amylase messenger RNA progressively decreases in rats rendered diabetic with streptozotocin. (elsevier.com)
- Pancreatic Amylase Alpha Monoclonal antibody specifically detects Pancreatic Amylase Alpha in Rat samples. (fishersci.com)
- Pancreatic Amylase Alpha Monoclonal antibody specifically detects Pancreatic Amylase Alpha in Human samples. (fishersci.com)
- Western Blot (WB) : This antibody detects a major band of approximately 56 kDa in porcine amylase samples under reducing conditions. (genetex.com)
- More than 95% of the alpha-amylase activity in the pancreas and in the tumour was absorbed by an excess amount of either antibody, indicating that normal and tumour alpha-amylases are immunologically identical. (curehunter.com)
- The antibody detects the Rat Amylase at the expected molecular weight i.e. at ~54kDa in the pancreatic lysate and recombinant protein at ~ 60 kDa. (acris-antibodies.com)
- 1 µg of coated antigen can be sensitively detected by anti-Rat Pancreatic Amylase antibody, clone B5 used at 30 pg/well concentration. (acris-antibodies.com)
- Mouse pancreatic sections were fixed in 4% paraformaldehyde and used for immunohistochemistry using rabbit anti-amylase antibody at a dilution of 1:100. (sigmaaldrich.com)
- Amylase is produced mainly by the exocrine pancreas (P-type) and the salivary glands (S-type)but it is also found in other tissues. (sesmedikal.com)
- J.D. Gardner, Regulation of pancreatic exocrine function in vivo. (springer.com)
- pancreatic acinus one of the secretory units of the exocrine pancreas , where pancreatic juice is produced. (thefreedictionary.com)
- Glycosidases: amylase, alpha 2A (pancreatic). (guidetopharmacology.org)
- The fluids from the drain can be tested for amylase (one of the contents of the pancreatic juice which digests carbohydrates) to find out whether the fluid in the drain is because of a pancreatic leak. (cochrane.org)
- The amylases are a group of hydrolases that split complex carbohydrates constituted of I D glucose units. (randox.com)
- Amylase, produced by the salivary glands, breaks complex carbohydrates to smaller chains, or even simple sugars. (wikipedia.org)
- However, no research has been conducted to study its effect on the activity of porcine pancreatic alpha-amylase (PPA), which is involved in carbohydrate absorption. (preprints.org)
- Here we report on the structures of three dromedary VHH domains in complex with porcine pancreatic alpha-amylase. (proteopedia.org)
- Cytosolic free calcium concentrations ([Ca2+]i) and amylase secretion were measured in isolated rat pancreatic acini loaded with the intracellularly trapped fluorescent indicator quin2. (biochemj.org)
- Specifically, we examined the ability of the muscarinic agonist carbachol to stimulate amylase secretion in vitro, using dispersed pancreatic acini prepared from wild-type and mAChR mutant mice. (aspetjournals.org)
- Quantitative reverse transcription-polymerase chain reaction studies using RNA prepared from mouse pancreatic acini showed that deletion of the M 1 or M 3 mAChR genes did not lead to significantly altered mRNA levels of the remaining mAChR subtypes. (aspetjournals.org)
- Moreover, immunoprecipitation studies with M 1 and M 3 mAChR-selective antisera demonstrated that both mAChR subtypes are expressed by mouse pancreatic acini. (aspetjournals.org)
- However, it has been unclear whether GLP-1R is present in pancreatic acini and what physiological functions these receptors regulate. (nih.gov)
- RNA expression of GLP-1R was detected in acutely isolated pancreatic acini. (nih.gov)
- GLP-1 induced amylase secretion in wild-type (WT) acini. (nih.gov)
- In GLP-1R KO mice, this effect was abolished, whereas vasoactive intestinal peptide-induced amylase release in KO acini showed a pattern similar to that in WT acini. (nih.gov)
- pancreatic acini. (nih.gov)
- The effect of estradiol (E 2 ) on amylase content and on basal and stimulated amylase release from the pancreatic acini was examined in relation to its effects on cholecystokinin (CCK)-receptor (R) levels. (utmb.edu)
- After 7 days of injections, animals were killed, pancreata weighed and basal and stimulated amylase release from pancreatic acini measured. (utmb.edu)
- To characterize the changes in pancreatic function during postnatal development, isolated pancreatic acini were prepared from rats aged 8-9, 12-14 and 20 days and from adult rats. (elsevier.com)
- These data indicate that viable isolated pancreatic acini can be prepared from suckling rats and that these acini exhibit an altered in vitro responsiveness to carbachol. (elsevier.com)
- These modified substrates may be used for the detection and kinetic analysis of a-amylases, and are useful in rapidly screening for novel a-amylase inhibitors and for subsequent kinetic characterisation. (ubc.ca)
- Finally, Glu233, Gln63, His305, Asp300 and Tyr151 are predicted to be markers of important areas with which potential amylase inhibitors would interact. (preprints.org)
- A new approach for the discovery and subsequent structural elucidation of oligosaccharide-based inhibitors of alpha-amylases based upon autoglucosylation of known alpha-glucosidase inhibitors is presented. (nih.gov)
- This approach to the discovery and structural analysis of amylase inhibitors should be generally applicable to other endoglucosidases and readily adaptable to a high throughput format. (nih.gov)
- In contrast with complexes of other proteinaceous amylase inhibitors, amylase kept its native structure. (proteopedia.org)
- they need to first be broken down by either salivary or pancreatic amylase into small polymers (disaccharides) of maltose, and then the maltose is broken down by what else, maltase, into two molecules of glucose. (infoplease.com)
- The methanolic extract was further quantified with respect to intestinal α-glucosidase (maltase and sucrase) and pancreatic α-amylase inhibition by glucose oxidase method and Dinitrosalicylic acid (DNSA) reagent, respectively. (jbiochemtech.com)
- Salivary and pancreatic amylases (encoded by AMY1 and AMY2 genes, respectively) are responsible for digesting starchy foods. (diabetesjournals.org)
- AMY1 and AMY2 show copy number variations that affect differences in amylase amount and activity, and AMY1 copies have been associated with adiposity. (diabetesjournals.org)
- Overweight and obese individuals carrying the AMY1-AMY2 rs11185098 genotype associated with higher amylase activity may have greater loss of adiposity during low-calorie diet interventions. (diabetesjournals.org)
- Salivary α-amylase is encoded by the salivary amylase gene ( AMY1 ), which shows extensive copy number variations ( 4 - 7 ) that directly affect individual variability in salivary amylase amount and activity ( 8 - 10 ) as well as serum concentrations of total and pancreatic amylase ( 11 ). (diabetesjournals.org)
- Cancer of the pancreas, ovaries or lungs, pancreatic or bile duct blockage, gallbladder attacks caused by disease, intestinal blockages, perforated ulcers and tubal pregnancies may cause increased levels of amylase in the blood, states MedlinePlus. (reference.com)
- seeds on intestinal α-glucosidase and pancreatic α-amylase. (jbiochemtech.com)
- These results show that caerulein and carbamoylcholine, two agents thought to activate secretion mainly through mobilization of Ca2+ from intracellular stores, are capable of eliciting amylase secretion independently of a concomitant rise in [Ca2+]i. (biochemj.org)
- This is due to the hormonal control of pancreatic secretions. (infoplease.com)
- Pancreatic amylase occurs in serum at a concentration of about 60µg/L, from a total amylase concentration in serum of about 120µg/L. Pancreatic amylase and salivary amylase are the products of separate genes and, although they share 98% amino acid sequence homology, they differ in molecular size, isoelectric point and antigenic properties. (abcam.com)
- The evaluation of the pancreatic isoamylase may have a greater clinical specificity for the diagnosis of pancreatic disorders than total amylase assessment. (randox.com)
- Concern has been expressed about the use of serum amylase to diagnose pancreatitis. (bestbets.org)
- cases of increased serum amylase without obvious pancreatitis or parotitis require quantification of amylase isoenzymes. (thefreedictionary.com)
- Doctors may order an amylase test, in which samples of either blood or urine are taken, for patients who have or display the symptoms of acute pancreatitis, chronic pancreatitis or pancreatic pseuodocysts. (reference.com)
- Measurement of amylase concentrations in serum is a diagnostic test for various pancreatic disorders (especially acute and relapsing pancreatitis). (abcam.com)
- Amylase measurements are used primarily for the diagnosis and treatment of pancreatitis (inflammation of the pancreas). (randox.com)
- Assays of amylase activity in serum and urine are largely of use in the diagnosis of pancreatic diseases such as acute or chronic pancreatitis. (sesmedikal.com)
- Avoiding alcohol, and taking all the prescribed medications are the primary treatments for high blood amylase levels, if you are being followed in the outpatient department, and you have not been diagnosed with any kind of pancreatitis. (chemocare.com)
- Acute pancreatitis has also been reported in patients with a normal amylase. (bestbets.org)
- How can you diagnose pancreatic necrosis due to chronic pancreatitis? (healthtap.com)
- Measurement of serum α-amylase activity is an important diagnostic test for acute and chronic pancreatitis. (thefreedictionary.com)
- Chronic pancreatitis often masquerades as psychoneurosis, drug addiction, or chronic alcoholism, while pancreatic carcinoma is usually beyond surgical cure when diagnosed. (annals.org)
- For the quantitative in vitro determination of Pancreatic Amylase in serum, plasma and urine. (randox.com)
- This preparation should therefore be a useful model for in vitro studies of the development of pancreatic function. (elsevier.com)
- Exposure of C. jejuni to pancreatic amylase promotes biofilm formation in vitro, increases interaction with human epithelial cell lines, increases virulence in the Galleria mellonella infection model, and promotes colonization of the chicken ileum. (hud.ac.uk)
- We also show that exposure to pancreatic amylase protects C. jejuni from stress conditions in vitro, suggesting that the induced α-dextran may be important during transmission between hosts. (hud.ac.uk)
- Diagnosis of Pancreatic Disease with Radioselenomethionine Photoscanning. (annals.org)
- Amylase catalyzes the hydrolysis of 2-chloro-4-nitrophenyl-malto-trioside (CNP-G3) to 2-chloro-4-nitrophenol (CNP). (sesmedikal.com)
- In this paper, we used fluorescence PPA inhibition assay to investigate the inhibitory effects of RCM-107 and the individual herbs present in this herbal mixture on amylase activity. (preprints.org)
- In addition, alisol B, (-)-epigallocatechin-3-gallate (EGCG) and plantagoside have been predicted as the key active compounds that may be responsible for the α-amylase inhibition effect of RCM-107 according to inter-residue contact analysis. (preprints.org)
- In this study, seventeen Indian medicinal plants with known hypoglycemic properties were subjected to sequential solvent extraction and tested for α-amylase inhibition, in order to assess and evaluate their inhibitory potential on PPA (porcine pancreatic α-amylase). (biomedcentral.com)
- Amylase is normally secreted from the pancreas through the pancreatic duct to the small intestines. (medlineplus.gov)
- Alternative catalytic anions differentially modulate human alpha-amylase activity and specificity. (ebi.ac.uk)
- Human I amylase consists of two major isoenzymes, pancreatic and salivary, which are encoded by two different genes. (randox.com)
- The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. (wikipedia.org)
- The numbers of salivary amylase genes in some families showing quantitative variation at the protein level have been estimated by the polymerase chain reaction. (springer.com)
- Groot PC, Bleeker MJ, Pronk JC, Arwert F, Mager WH, Planta RJ, Eriksson AW, Frants RR (1989) The human α-amylase multigene family consists of haplotypes with variable numbers of genes. (springer.com)
- 123111 - Amylase is primarily produced in the pancreas and salivary glands. (mcssl.com)
- Bisdemethoxycurcumin from Curcuma longa rhizome is a potent small molecule inhibitor of human pancreatic α-amylase, a target for type-2 diabetes. (greenmedinfo.com)
- This was transformed from an inhibitor of human pancreatic alpha-amylase with a K(i) value of 18 mm to a trisaccharide analogue with a K(i) value of 25 mum. (nih.gov)
- Acute pancreatic disease must be differentiated from a variety of intra-abdominal catastrophes, many of which require prompt laparotomy if the patient is to have the best chance of survival. (annals.org)
- Although amino acid analysis and peptide mapping with the use of CNBr, N-chlorosuccinimide or Staphylococcus aureus V8 proteinase gave comparable profiles for the two alpha-amylases , tryptic-digest fingerprint patterns were different. (curehunter.com)
- These amylases share a 97% - 99% amino acid sequence identity, and two potential N-glycosylation sites (N427 and N476) are commonly found in the C-terminal region. (scirp.org)
- Amylases cleave the glycosidic bonds of the long polysaccharide chains, producing a mixture of glucose and maltose. (encyclopedia.com)
- In those with high amylase, insulin increased and blood glucose didn't spike. (selfhacked.com)
- But in those with low amylase, insulin didn't increase and as a result blood glucose did [ 8 ]. (selfhacked.com)
- Amylase doesn't affect blood glucose levels after eating simple sugars, like glucose or sucrose. (selfhacked.com)
- Both people with high and low amylase will react the same to glucose [ 8 ]. (selfhacked.com)
- The water-accessible surface areas of VHHs covered by amylase ranged between 850 and 1150 A(2), values similar to or even larger than those observed in the complexes between proteins and classical antibodies. (proteopedia.org)
- Pronk JC, Frants RR, Jansen W, Eriksson AW, Tonino GJM (1982) Evidence for the duplication of the human salivary amylase gene. (springer.com)
- stimulated amylase secretion was significantly less in the 8- to 9- and 12- to 14-day-old animals than in the 20-day-old and adult rats. (elsevier.com)
- Measurement of α-amylase in serum and urine is mainly applied for the diagnosis of pancreatic disorders and monitoring complications. (diasys-diagnostics.com)
- Reagent for the measurement of -amylase concentration in human serum, plasma or urine. (sesmedikal.com)
- In this study, we evaluated the glycosylation state of human amylase isozymes and found that the different glycosylation patterns of human amylases were related to the types of amylase-producing cells rather than the types of amylase isozymes. (scirp.org)
- There are two main types of amylase - salivary and pancreatic [ 1 , 2 ]. (selfhacked.com)
- However, caerulein (10(-12) M and 10(-11) M) as well as carbamoylcholine (10(-7) M) caused a significant stimulation of amylase release, while not inducing any detectable rise in [Ca2+]i. (biochemj.org)
- Furthermore, with both secretagogues the rise in [Ca2+]i, when observed, was only transient, while the stimulation of amylase release was sustained. (biochemj.org)
- Derivatives of maltose and maltotriose were chemically synthesised as substrates for human pancreatic alpha-amylases and subjected to kinetic analysis. (ubc.ca)
- Your search returned 4 amylase, alpha 1B (salivary) ELISA ELISA Kit across 2 suppliers. (biocompare.com)
- These findings led to the conclusion that the liver contributes to the blood amylase level and is an important site of regulation. (annals.org)
- Our Pancreatic Amylase Beta RNAi can be used in a variety of model species: Human. (novusbio.com)
- Pancreatic secretory granule membrane major glycoprotein GP2 is a protein that in humans is encoded by the GP2 gene. (wikipedia.org)
- We present evidence that quantitative variations in amylase protein patterns do not always reflect variations in gene copy number but that other mechanisms are also involved. (springer.com)
- How to diagnose pancreatic dysfunction? (healthtap.com)
- The mechanism of allosteric activation of alpha-amylase by chloride has been studied through structural and kinetic experiments focusing on the chloride-dependent N298S variant of human pancreatic alpha-amylase (HPA) and a chloride-independent TAKA-amylase. (ubc.ca)
- amylase (e.g. plant and bacterial exoamylase) and human I amylases which can attack the I 1, 4 linkage anywhere along a polyglucan chain. (randox.com)
- The polymorphic patterns of human salivary amylase of a large number of individuals of Caucasian origin were determined by using isoelectric focusing and polyacrylamide gel electrophoresis. (springer.com)
- Bank RA, Hettema EH, Arwert F, Nieuw Amerongen AV, Pronk JC (1991) Electrophoretic characterization of posttranslational modifications of human parotid salivary α-amylase. (springer.com)
- Eckersall PD, Beeley JA (1981) Genetic analysis of human salivary α-amylase isozymes by isoelectric focusing. (springer.com)
- Kauffman DL, Watanabe S, Evans JR, Keller PJ (1973) The existance of glycosylated and non-glycosylated forms of human submandibular amylase. (springer.com)
- Merritt AD, Karn RC (1977) The human α-amylases. (springer.com)
- Nishide T, Emi M, Nakamura Y, Matsubara K (1986) Corrected sequences of cDNAs for human salivary and pancreatic αamylases. (springer.com)
- Ogita S (1966) Genetico-biochemical studies on the salivary and pancreatic amylase isozymes in human. (springer.com)
- In general, salivary amylase is more frequently glycosylated than pancreatic amylase, and it is still uncertain why differences in the glycosylation pattern among human amylase isozymes occur. (scirp.org)
- The difference in the glycosylation efficiency of each N-glycosylation site also seemed to contribute in part to generate different glycosylation patterns of human amylases. (scirp.org)
- We also confirmed that the C-terminal region of human amylase plays a critical role in secretion, although glycosylation does not play a part in this effect. (scirp.org)
- The N-glycan structures of human amylase from the parotid are complex-type glycans with several glycoforms . (scirp.org)
- We studied the structures of N-glycans of human pancreatic amylase and found that they are different from those of salivary amylase (Oyama et al. (scirp.org)
- The N-glycan structures of human amylase from tumors were similar but modified compared to those of parotid amylase [3, (scirp.org)
- In addition to the structural variety of N-glycans, there is a difference in the glycosylation pattern of human amylase isozymes [5, both the N427 and N476 sites in human salivary amylase can be glycosylated to some extent, whereas those in human pancreatic amylase are either glycosylated to a lesser extent or not glycosylated. (scirp.org)
- In the case of human amylase, glycosylation may not be important since the unglycosylated form of amylases is fully secreted and active. (scirp.org)
- It is still uncertain, however, why differences in the glycosylation pattern among human amylase isozymes occur. (scirp.org)
- These findings seem to be highly related to the different glycosylation pattern of human amylases. (scirp.org)
- In addition, we discuss the importance of the C-terminal region of human amylase in secretion. (scirp.org)
- Human pancreatic amylase was purchased from Meridian Life Science, Inc. (Saco, ME). (scirp.org)
- human salivary α-amylase. (sigmaaldrich.com)