An albumin obtained from the white of eggs. It is a member of the serpin superfamily.
Ducts that serve exclusively for the passage of eggs from the ovaries to the exterior of the body. In non-mammals, they are termed oviducts. In mammals, they are highly specialized and known as FALLOPIAN TUBES.
Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.
A COUP transcription factor that was originally identified as a homodimer that binds to a direct repeat regulatory element in the chicken albumin promoter. It is a transcription factor that plays an important role in EMBRYONIC DEVELOPMENT of the CENTRAL NERVOUS SYSTEM.
An immunoglobulin associated with MAST CELLS. Overexpression has been associated with allergic hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).
Antigen-type substances that produce immediate hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).
Tendency of the smooth muscle of the tracheobronchial tree to contract more intensely in response to a given stimulus than it does in the response seen in normal individuals. This condition is present in virtually all symptomatic patients with asthma. The most prominent manifestation of this smooth muscle contraction is a decrease in airway caliber that can be readily measured in the pulmonary function laboratory.
A COUP transcription factor that negatively regulates GENETIC TRANSCRIPTION and competes with other hormone receptors for the common response element AGGTCA. It can also stimulate transcription of genes involved in the metabolism of GLUCOSE and CHOLESTEROL.
A sub-family of steroid receptor-related orphan nuclear receptors that have specificity for a variety of DNA sequences related to AGGTCA. COUP transcription factors can heterodimerize with a variety of factors including RETINOIC ACID RECEPTORS; THYROID HORMONE RECEPTORS; and VITAMIN D RECEPTORS.
Substances that are recognized by the immune system and induce an immune reaction.
A form of hypersensitivity affecting the respiratory tract. It includes ASTHMA and RHINITIS, ALLERGIC, SEASONAL.
A glycoprotein albumin from hen's egg white with strong iron-binding affinity.
Washing liquid obtained from irrigation of the lung, including the BRONCHI and the PULMONARY ALVEOLI. It is generally used to assess biochemical, inflammatory, or infection status of the lung.
A heterogeneous mixture of glycoproteins responsible for the gel structure of egg white. It has trypsin-inhibiting activity.
A form of bronchial disorder with three distinct components: airway hyper-responsiveness (RESPIRATORY HYPERSENSITIVITY), airway INFLAMMATION, and intermittent AIRWAY OBSTRUCTION. It is characterized by spasmodic contraction of airway smooth muscle, WHEEZING, and dyspnea (DYSPNEA, PAROXYSMAL).
Altered reactivity to an antigen, which can result in pathologic reactions upon subsequent exposure to that particular antigen.
Granular leukocytes with a nucleus that usually has two lobes connected by a slender thread of chromatin, and cytoplasm containing coarse, round granules that are uniform in size and stainable by eosin.
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
The white of an egg, especially a chicken's egg, used in cooking. It contains albumin. (Random House Unabridged Dictionary, 2d ed)
A quaternary ammonium parasympathomimetic agent with the muscarinic actions of ACETYLCHOLINE. It is hydrolyzed by ACETYLCHOLINESTERASE at a considerably slower rate than ACETYLCHOLINE and is more resistant to hydrolysis by nonspecific CHOLINESTERASES so that its actions are more prolonged. It is used as a parasympathomimetic bronchoconstrictor agent and as a diagnostic aid for bronchial asthma. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1116)
A synthetic nonsteroidal estrogen used in the treatment of menopausal and postmenopausal disorders. It was also used formerly as a growth promoter in animals. According to the Fourth Annual Report on Carcinogens (NTP 85-002, 1985), diethylstilbestrol has been listed as a known carcinogen. (Merck, 11th ed)
Gastrointestinal disturbances, skin eruptions, or shock due to allergic reactions to allergens in food.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
A cytokine that promotes differentiation and activation of EOSINOPHILS. It also triggers activated B-LYMPHOCYTES to differentiate into IMMUNOGLOBULIN-secreting cells.
An evanescent cutaneous reaction occurring when antibody is injected into a local area on the skin and antigen is subsequently injected intravenously along with a dye. The dye makes the rapidly occurring capillary dilatation and increased vascular permeability readily visible by leakage into the reaction site. PCA is a sensitive reaction for detecting very small quantities of antibodies and is also a method for studying the mechanisms of immediate hypersensitivity.
A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.
An increased reactivity to specific antigens mediated not by antibodies but by cells.
An acute hypersensitivity reaction due to exposure to a previously encountered ANTIGEN. The reaction may include rapidly progressing URTICARIA, respiratory distress, vascular collapse, systemic SHOCK, and death.
Subset of helper-inducer T-lymphocytes which synthesize and secrete the interleukins IL-4, IL-5, IL-6, and IL-10. These cytokines influence B-cell development and antibody production as well as augmenting humoral responses.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
Substances that augment, stimulate, activate, potentiate, or modulate the immune response at either the cellular or humoral level. The classical agents (Freund's adjuvant, BCG, Corynebacterium parvum, et al.) contain bacterial antigens. Some are endogenous (e.g., histamine, interferon, transfer factor, tuftsin, interleukin-1). Their mode of action is either non-specific, resulting in increased immune responsiveness to a wide variety of antigens, or antigen-specific, i.e., affecting a restricted type of immune response to a narrow group of antigens. The therapeutic efficacy of many biological response modifiers is related to their antigen-specific immunoadjuvanticity.
The structural changes in the number, mass, size and/or composition of the airway tissues.
Globulins of milk obtained from the WHEY.
A glandular epithelial cell or a unicellular gland. Goblet cells secrete MUCUS. They are scattered in the epithelial linings of many organs, especially the SMALL INTESTINE and the RESPIRATORY TRACT.
Proteins which are found in eggs (OVA) from any species.
Agents causing the narrowing of the lumen of a bronchus or bronchiole.
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
Physiologically, the opposition to flow of air caused by the forces of friction. As a part of pulmonary function testing, it is the ratio of driving pressure to the rate of air flow.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
A condition characterized by infiltration of the lung with EOSINOPHILS due to inflammation or other disease processes. Major eosinophilic lung diseases are the eosinophilic pneumonias caused by infections, allergens, or toxic agents.
Narrowing of the caliber of the BRONCHI, physiologically or as a result of pharmacological intervention.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
A family of serine proteinase inhibitors which are similar in amino acid sequence and mechanism of inhibition, but differ in their specificity toward proteolytic enzymes. This family includes alpha 1-antitrypsin, angiotensinogen, ovalbumin, antiplasmin, alpha 1-antichymotrypsin, thyroxine-binding protein, complement 1 inactivators, antithrombin III, heparin cofactor II, plasminogen inactivators, gene Y protein, placental plasminogen activator inhibitor, and barley Z protein. Some members of the serpin family may be substrates rather than inhibitors of SERINE ENDOPEPTIDASES, and some serpins occur in plants where their function is not known.
Agents that are used to treat allergic reactions. Most of these drugs act by preventing the release of inflammatory mediators or inhibiting the actions of released mediators on their target cells. (From AMA Drug Evaluations Annual, 1994, p475)
An encapsulated lymphatic organ through which venous blood filters.
A soluble factor produced by activated T-LYMPHOCYTES that induces the expression of MHC CLASS II GENES and FC RECEPTORS on B-LYMPHOCYTES and causes their proliferation and differentiation. It also acts on T-lymphocytes, MAST CELLS, and several other hematopoietic lineage cells.
Abnormal increase of EOSINOPHILS in the blood, tissues or organs.
The process by which antigen is presented to lymphocytes in a form they can recognize. This is performed by antigen presenting cells (APCs). Some antigens require processing before they can be recognized. Antigen processing consists of ingestion and partial digestion of the antigen by the APC, followed by presentation of fragments on the cell surface. (From Rosen et al., Dictionary of Immunology, 1989)
Phthalic acid anhydrides. Can be substituted on any carbon atom. Used extensively in industry and as a reagent in the acylation of amino- and hydroxyl groups.
Infection of the lung often accompanied by inflammation.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
A cytokine synthesized by T-LYMPHOCYTES that produces proliferation, immunoglobulin isotype switching, and immunoglobulin production by immature B-LYMPHOCYTES. It appears to play a role in regulating inflammatory and immune responses.
The giving of drugs, chemicals, or other substances by mouth.
Spasmodic contraction of the smooth muscle of the bronchi.
Delivery of medications through the nasal mucosa.
A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.
The viscous secretion of mucous membranes. It contains mucin, white blood cells, water, inorganic salts, and exfoliated cells.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Allergic reaction to eggs that is triggered by the immune system.
An amine derived by enzymatic decarboxylation of HISTIDINE. It is a powerful stimulant of gastric secretion, a constrictor of bronchial smooth muscle, a vasodilator, and also a centrally acting neurotransmitter.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The cartilaginous and membranous tube descending from the larynx and branching into the right and left main bronchi.
Tests involving inhalation of allergens (nebulized or in dust form), nebulized pharmacologically active solutions (e.g., histamine, methacholine), or control solutions, followed by assessment of respiratory function. These tests are used in the diagnosis of asthma.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
The tubular and cavernous organs and structures, by means of which pulmonary ventilation and gas exchange between ambient air and the blood are brought about.
Colloids with a gaseous dispersing phase and either liquid (fog) or solid (smoke) dispersed phase; used in fumigation or in inhalation therapy; may contain propellant agents.
Proteins found usually in the cytoplasm or nucleus that specifically bind steroid hormones and trigger changes influencing the behavior of cells. The steroid receptor-steroid hormone complex regulates the transcription of specific genes.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
Aluminum metal sulfate compounds used medically as astringents and for many industrial purposes. They are used in veterinary medicine for the treatment of ulcerative stomatitis, leukorrhea, conjunctivitis, pharyngitis, metritis, and minor wounds.
Differential thermal analysis in which the sample compartment of the apparatus is a differential calorimeter, allowing an exact measure of the heat of transition independent of the specific heat, thermal conductivity, and other variables of the sample.

Interleukin-8 receptor modulates IgE production and B-cell expansion and trafficking in allergen-induced pulmonary inflammation. (1/4583)

We examined the role of the interleukin-8 (IL-8) receptor in a murine model of allergen-induced pulmonary inflammation using mice with a targeted deletion of the murine IL-8 receptor homologue (IL-8r-/-). Wild-type (Wt) and IL-8r-/- mice were systemically immunized to ovalbumin (OVA) and were exposed with either single or multiple challenge of aerosolized phosphate-buffered saline (OVA/PBS) or OVA (OVA/OVA). Analysis of cells recovered from bronchoalveolar lavage (BAL) revealed a diminished recruitment of neutrophils to the airway lumen after single challenge in IL-8r-/- mice compared with Wt mice, whereas multiply challenged IL-8r-/- mice had increased B cells and fewer neutrophils compared with Wt mice. Both Wt and IL-8r-/- OVA/OVA mice recruited similar numbers of eosinophils to the BAL fluid and exhibited comparable degrees of pulmonary inflammation histologically. Both total and OVA-specific IgE levels were greater in multiply challenged IL-8r-/- OVA/OVA mice than in Wt mice. Both the IL-8r-/- OVA/OVA and OVA/PBS mice were significantly less responsive to methacholine than their respective Wt groups, but both Wt and IL-8r mice showed similar degrees of enhancement after multiple allergen challenge. The data demonstrate that the IL-8r modulates IgE production, airway responsiveness, and the composition of the cells (B cells and neutrophils) recruited to the airway lumen in response to antigen.  (+info)

Prolonged eosinophil accumulation in allergic lung interstitium of ICAM-2 deficient mice results in extended hyperresponsiveness. (2/4583)

ICAM-2-deficient mice exhibit prolonged accumulation of eosinophils in lung interstitium concomitant with a delayed increase in eosinophil numbers in the airway lumen during the development of allergic lung inflammation. The ICAM-2-dependent increased and prolonged accumulation of eosinophils in lung interstitium results in prolonged, heightened airway hyperresponsiveness. These findings reveal an essential role for ICAM-2 in the development of the inflammatory and respiratory components of allergic lung disease. This phenotype is caused by the lack of ICAM-2 expression on non-hematopoietic cells. ICAM-2 deficiency on endothelial cells causes reduced eosinophil transmigration in vitro. ICAM-2 is not essential for lymphocyte homing or the development of leukocytes, with the exception of megakaryocyte progenitors, which are significantly reduced.  (+info)

Zonula occludens toxin is a powerful mucosal adjuvant for intranasally delivered antigens. (3/4583)

Zonula occludens toxin (Zot) is produced by toxigenic strains of Vibrio cholerae and has the ability to reversibly alter intestinal epithelial tight junctions, allowing the passage of macromolecules through the mucosal barrier. In the present study, we investigated whether Zot could be exploited to deliver soluble antigens through the nasal mucosa for the induction of antigen-specific systemic and mucosal immune responses. Intranasal immunization of mice with ovalbumin (Ova) and recombinant Zot, either fused to the maltose-binding protein (MBP-Zot) or with a hexahistidine tag (His-Zot), induced anti-Ova serum immunoglobulin G (IgG) titers that were approximately 40-fold higher than those induced by immunization with antigen alone. Interestingly, Zot also stimulated high anti-Ova IgA titers in serum, as well as in vaginal and intestinal secretions. A comparison with Escherichia coli heat-labile enterotoxin (LT) revealed that the adjuvant activity of Zot was only sevenfold lower than that of LT. Moreover, Zot and LT induced similar patterns of Ova-specific IgG subclasses. The subtypes IgG1, IgG2a, and IgG2b were all stimulated, with a predominance of IgG1 and IgG2b. In conclusion, our results highlight Zot as a novel potent mucosal adjuvant of microbial origin.  (+info)

Anaphylactic bronchoconstriction in BP2 mice: interactions between serotonin and acetylcholine. (4/4583)

1. Immunized BP2 mice developed an acute bronchoconstriction in vivo and airway muscle contraction in vitro in response to ovalbumin (OA) and these contractions were dose dependent. 2. Methysergide or atropine inhibited OA-induced bronchoconstriction in vivo and airway muscle contraction in vitro. 3. Neostigmine potentiated the OA-induced bronchoconstriction in vivo and airway muscle contraction in vitro of BP2 mice. This potentiation was markedly reduced by the administration of methysergide or atropine and when the two antagonists were administered together, the responses were completely inhibited. 4. Neostigmine also potentiated the serotonin (5-HT)- and acetylcholine (ACh)-induced bronchoconstriction and this potentiation was significantly reversed by atropine. 5. These results indicate that OA provokes a bronchoconstriction in immunized BP2 mice by stimulating the release of 5-HT, which in turn acts via the cholinergic mediator, ACh.  (+info)

Stabilization of L-ascorbic acid by superoxide dismutase and catalase. (5/4583)

The effects of superoxide dismutase (SOD) and catalase on the autoxidation rate of L-ascorbic acid (ASA) in the absence of metal ion catalysts were examined. The stabilization of ASA by SOD was confirmed, and the enzyme activity of SOD, which scavenges the superoxide anion formed during the autoxidation of ASA, contributed strongly to this stabilization. The stabilization of ASA by catalase was observed for the first time; however, the specific enzyme ability of catalase would not have been involved in the stabilization of ASA. Such proteins as bovine serum albumin (BSA) and ovalbumin also inhibited the autoxidation of ASA, therefore it seems that non-specific interaction between ASA and such proteins as catalase and BSA might stabilize ASA and that the non-enzymatic superoxide anion scavenging ability of proteins might be involved.  (+info)

Contributory and exacerbating roles of gaseous ammonia and organic dust in the etiology of atrophic rhinitis. (6/4583)

Pigs reared commercially indoors are exposed to air heavily contaminated with particulate and gaseous pollutants. Epidemiological surveys have shown an association between the levels of these pollutants and the severity of lesions associated with the upper respiratory tract disease of swine atrophic rhinitis. This study investigated the role of aerial pollutants in the etiology of atrophic rhinitis induced by Pasteurella multocida. Forty, 1-week-old Large White piglets were weaned and divided into eight groups designated A to H. The groups were housed in Rochester exposure chambers and continuously exposed to the following pollutants: ovalbumin (groups A and B), ammonia (groups C and D), ovalbumin plus ammonia (groups E and F), and unpolluted air (groups G and H). The concentrations of pollutants used were 20 mg m-3 total mass and 5 mg m-3 respirable mass for ovalbumin dust and 50 ppm for ammonia. One week after exposure commenced, the pigs in groups A, C, E, and G were infected with P. multocida type D by intranasal inoculation. After 4 weeks of exposure to pollutants, the pigs were killed and the extent of turbinate atrophy was assessed with a morphometric index (MI). Control pigs kept in clean air and not inoculated with P. multocida (group H) had normal turbinate morphology with a mean MI of 41.12% (standard deviation [SD], +/- 1. 59%). In contrast, exposure to pollutants in the absence of P. multocida (groups B, D, and F) induced mild turbinate atrophy with mean MIs of 49.65% (SD, +/-1.96%), 51.04% (SD, +/-2.06%), and 49.88% (SD, +/-3.51%), respectively. A similar level of atrophy was also evoked by inoculation with P. multocida in the absence of pollutants (group G), giving a mean MI of 50.77% (SD, +/-2.07%). However, when P. multocida inoculation was combined with pollutant exposure (groups A, C, and E) moderate to severe turbinate atrophy occurred with mean MIs of 64.93% (SD, +/-4.64%), 59.18% (SD, +/-2.79%), and 73.30% (SD, +/-3.19%), respectively. The severity of atrophy was greatest in pigs exposed simultaneously to dust and ammonia. At the end of the exposure period, higher numbers of P. multocida bacteria were isolated from the tonsils than from the nasal membrane, per gram of tissue. The severity of turbinate atrophy in inoculated pigs was proportional to the number of P. multocida bacteria isolated from tonsils (r2 = 0.909, P < 0.05) and nasal membrane (r2 = 0.628, P < 0.05). These findings indicate that aerial pollutants contribute to the severity of lesions associated with atrophic rhinitis by facilitating colonization of the pig's upper respiratory tract by P. multocida and also by directly evoking mild atrophy.  (+info)

Compliance and stability of the bronchial wall in a model of allergen-induced lung inflammation. (7/4583)

Airway wall remodeling in response to inflammation might alter load on airway smooth muscle and/or change airway wall stability. We therefore determined airway wall compliance and closing pressures in an animal model. Weanling pigs were sensitized to ovalbumin (OVA; ip and sc, n = 6) and were subsequently challenged three times with OVA aerosol. Control pigs received 0.9% NaCl (n = 4) in place of OVA aerosol. Bronchoconstriction in vivo was assessed from lung resistance and dynamic compliance. Semistatic airway compliance was recorded ex vivo in isolated segments of bronchus, after the final OVA aerosol or 0.9% NaCl challenge. Internally or externally applied pressure needed to close bronchial segments was determined in the absence or presence of carbachol (1 microM). Sensitized pig lungs exhibited immediate bronchoconstriction to OVA aerosol and also peribronchial accumulations of monocytes and granulocytes. Compliance was reduced in sensitized bronchi in vitro (P < 0.01), and closing pressures were increased (P < 0.05). In the presence of carbachol, closing pressures of control and sensitized bronchi were not different. We conclude that sensitization and/or inflammation increases airway load and airway stability.  (+info)

Qualitative and quantitative differences in T cell receptor binding of agonist and antagonist ligands. (8/4583)

The kinetics of interaction between TCR and MHC-peptide show a general relationship between affinity and the biological response, but the reported kinetic differences between antigenic and antagonistic peptides are very small. Here, we show a remarkable difference in the kinetics of TCR interactions with strong agonist ligands at 37 degrees C compared to 25 degrees C. This difference is not seen with antagonist/positive selecting ligands. The interaction at 37 degrees C shows biphasic binding kinetics best described by a model of TCR dimerization. The altered kinetics greatly increase the stability of complexes with agonist ligands, accounting for the large differences in biological response compared to other ligands. Thus, there may be an allosteric, as well as a kinetic, component to the discrimination between agonists and antagonists.  (+info)

Purified ovalbumin messenger RNA was employed to selectively enrich the concentration of the gene coding for ovalbumin from total chick DNA by molecular hybridization. The coding strand of the ovalbumin gene was partially purified from sheared chick DNA by affinity column chromatography using ovalbumin mRNA immobilized on phosphocellulose. The concentrations of the ovalbumin DNA sequence in various DNA fractions were quantitated by measuring their rates of hybridization with 125I-labeled ovalbumin mRNA. When apparent Cot1/2 values of these reactions were compared to the apparent Cot1/2 value obtained from the hybridization reaction between 125I-ovalbumin mRNA and complementary DNA synthesized against ovalbumin mRNA using the enzyme reverse transcriptase, purification of the coding ovalbumin DNA strand over total chick DNA was estimated to be approximately 9,600-fold. There was no apparent degradation of the 4,000 nucleotide strands of chick DNA throughout the purification procedure. Since ...
TY - JOUR. T1 - Translation of ovalbumin mRNA in Xenopus laevis oocytes. Characterization of the system and effects of estrogen on injected mRNA populations. AU - Chan, L.. AU - Kohler, Peter. AU - OMalley, B. W.. PY - 1976. Y1 - 1976. N2 - Ovalbumin messenger RNA (mRNA(ov)) purified from hen oviduct was injected into Xenopus laevis oocytes. The oocytes were incubated in culture medium containing [3H] leucine. Analysis of the oocyte cytosol on Sephadex G 150 columns demonstrated a peak of radioactivity which cochromatographed with authentic ovalbumin. Radioactive protein contained in this peak was precipitated by ovalbumin antiserum, co electrophoresed with ovalbumin on sodium dodecyl sulfate (SDS) and urea gels at pH 8.7, and eluted with the protein at the same pH (4.8) on CM cellulose chromatography. Injection of increasing amounts of mRNA(ov) was found to elicit a linear response in terms of ovalbumin synthesis. Moreover, there was linear incorporation of radioactivity into microinjected ...
Stein P.E., Leslie A.G.W., Finch J.T., Carrell R.W.. Ovalbumin, the major protein in avian egg-white, is a non-inhibitory member of the serine protease inhibitor (serpin) superfamily. The crystal structure of uncleaved, hen ovalbumin was solved by the molecular replacement method using the structure of plakalbumin, a proteolytically cleaved form of ovalbumin, as a starting model. The final refined model, including four ovalbumin molecules, 678 water molecules and a single metal ion, has a crystallographic R-factor of 17.4% for all reflections between 6.0 and 1.95 A resolution. The root-mean-square deviation from ideal values in bond lengths is 0.02 A and in bond angles is 2.9 degrees. This is the first crystal structure of a member of the serpin family in an uncleaved form. Surprisingly, the peptide that is homologous to the reactive centre of inhibitory serpins adopts an alpha-helical conformation. The implications for the mechanism of inhibition of the inhibitory members of the family is ...
A 17-amino acid tryptic peptide of chicken ovalbumin, designated P323-339, that substituted for processed antigen when presented by glutaraldehyde prefixed accessory cells to specific I-restricted T hybridomas was characterized. The peptide antigen could not be demonstrated to have any specific or stable interactions with accessory cell Ia antigens by either direct binding or functional assays for inhibition of specific T cell activation. In addition, the T cell receptor for I-restricted antigen had no affinity for free antigen alone. A rabbit antibody specific for the antigenic peptide inhibited presentation when introduced before but not after binding of the peptide to accessory cells. These results extend our earlier finding that accessory cell-mediated processing of chicken ovalbumin can be completely explained by the fragmentation of the native molecule into smaller m.w. peptides, and suggests that if an antigen/Ia complex is important in T cell activation, it forms significantly only in ...
TY - JOUR. T1 - Ovalbumin-sensitized mice are good models for airway hyperresponsiveness but not acute physiological responses to allergen inhalation. AU - Zosky, G. R.. AU - Larcombe, A. N.. AU - White, O. J.. AU - Burchell, J. T.. AU - Janosi, T. Z.. AU - Hantos, Z.. AU - Holt, P. G.. AU - Sly, P. D.. AU - Turner, D. J.. PY - 2008/5. Y1 - 2008/5. N2 - Background: Asthma is a chronic inflammatory disease that is characterized clinically by airway hyperresponsiveness (AHR) to bronchoconstricting agents. The physiological response of the asthmatic lung to inhaled allergen is often characterized by two distinct phases: an early-phase response (EPR) within the first hour following exposure that subsides and a late-phase response (LPR) that is more prolonged and may occur several hours later. Mouse models of asthma have become increasingly popular and should be designed to exhibit an EPR, LPR and AHR. Objective: To determine whether a common model of asthma is capable of demonstrating an EPR, LPR ...
Streptococcal mitogenic exotoxin Z-2 (SMEZ-2) is certainly a streptococcal superantigen that primarily stimulates human T cells bearing V8 and mouse T cells bearing V11. conjugated to ovalbumin (M1-ovalbumin) resulted in faster and quantitatively higher degrees of anti-ovalbumin IgG, Kaempferol supplier with endpoint titers getting 1,000- to 10,000-flip higher than those in pets immunized with unconjugated ovalbumin. Substantially higher degrees of anti-ovalbumin IgG had been seen in mice transgenic for individual main histocompatibility complicated (MHC) course II. Substitution of M1 with an MHC course II binding mutant (DM) removed enhanced immunity, recommending that M1 improved the delivery of antigen via MHC course II-positive antigen-presenting cells that predominate within lymphoid tissues. Immunization of pets using a conjugate comprising M1 and ovalbumin peptide from positions 323 Kaempferol supplier to 339 generated degrees of anti-peptide IgG 100-fold greater than those in pets ...
The early life period represents a time of immunological plasticity whereby the functionality immature immune system is highly susceptible to environmental stimulation. Perennial aeroallergen and respiratory viral infection induced sporadic episodes of lung inflammation during this temporal window represent major risk factors for initiation of allergic asthmatic disease. Murine models are widely used as an investigative tool to examine the pathophysiology of allergic asthma; however, models in current usage typically do not encapsulate the early life period which represents the time of maximal risk for disease inception in humans. To address this issue, this protocol adapted an experimental animal model of disease for sensitization to ovalbumin during the immediate post-weaning period beginning at 21 days of age. By initially sensitizing mice during this early life post-weaning period, researchers can more closely align experimental allergic. ...
Our experiments with Jα18−/− mice clearly show that iVα14 NKT lymphocytes are required for the characteristic features of experimental allergic asthma, namely airway inflammation, BALF Th2 cytokine production and the secretion of high levels of specific anti-OVA IgE, that will ultimely induce AHR.. The failure of Jα18−/− mice to develop the typical Th2 inflammation, AHR and IgE responses following OVA airway challenge is not due to a deficiency in Th2 responsiveness since these mice produce high levels of IgE in another protocol using three systemic immunizations with 2.5 higher doses of OVA. These data concur with previous reports showing that Jα18−/− mice are not defective in IgE production (15). In this study we used a protocol where all mice were similarly immunized with OVA and alum, and airway OVA challenge resulting in Th2 inflammation and AHR. Because we found that iVα14 NKT cells are present in the lung of OVA-challenged mice, it is possible that they are involved ...
The major goal of this study was to examine directly the capacity of Th1 cells to counterbalance the proasthmatic effects of Th2 cells in a murine model of asthma. The Th1/Th2 paradigm suggests that Th1 and Th2 cells counterbalance each other and that Th1 cells protect or prevent Th2-mediated allergic disease and asthma (27). However, using well-defined, phenotypically committed OVA-specific Th1 and Th2 cells expressing identical TCRs and adoptively transferred into either SCID mice or into OVA-immunized BALB/c mice, we found that OVA-specific Th1 cells failed to reverse Th2-mediated airway inflammation and airway hyperreactivity, even when given in twofold excess. These studies indicate that the Th1/Th2 paradigm, which predicts that Th1 cells downregulate allergic disease and asthma, may be more complex than initially appreciated and that suppression of allergic inflammation and Th2 activity in vivo may depend on cells other than Th1 lymphocytes.. Inasmuch as asthma is associated with the ...
The integrin CD103 is the αE chain of integrin αEβ7 that is important in the maintenance of intraepithelial lymphocytes and recruitment of T cells and dendritic cells (DC) to mucosal surfaces. The role of CD103 in intestinal immune homeostasis has been well described, however, its role in allergic airway inflammation is less well understood. In this study, we used an ovalbumin (OVA)-induced, CD103-knockout (KO) BALB/c mouse model of experimental allergic airways disease (EAAD) to investigate the role of CD103 in disease expression, CD4+ T-cell activation and DC activation and function in airways and lymph nodes. We found reduced airways hyper-responsiveness and eosinophil recruitment to airways after aerosol challenge of CD103 KO compared to wild-type (WT) mice, although CD103 KO mice showed enhanced serum OVA-specific IgE levels. Following aerosol challenge, total numbers of effector and regulatory CD4+ T-cell subsets were significantly increased in the airways of WT but not CD103 KO mice, ...
The primary objective of this study is to determine if broccoli sprouts (BS) improves airway inflammatory, oxidative stress (OS), and symptoms among asthmatic adults with aeroallergen sensitization.. The study is a double-blind, placebo-controlled, randomized trial to compare BS to placebo in 40 adults with asthma. 40 adults (age 18-50) who meet these eligibility criteria will be randomized to receive either: (a) BS or (b) placebo (alfalfa sprouts). Subjects will eat a sprouts sandwich daily for three days, and then undergo repeat measurement of outcomes. ...
This is the first report to suggest that in vivo inhibition of RhoA signalling may be a target for the treatment of asthma. Y-27632 significantly suppressed airway responsiveness induced by bronchospastic agonists in normal mice, allergen sensitised mice, and allergen sensitised mice infected with a virus.. Airway constriction caused by smooth muscle contraction is a critical feature of asthma. Histamine, neurokinin A, cysteinyl leukotriene, serotonin, methacholine, and acetylcholine are known bronchospastic agonists.22,23 Stimulation of their respective trimeric G protein-coupled receptors activates phospholipase C signalling pathways,24-26 leading to intracellular Ca2+ flux and smooth muscle contraction. Rho kinase inhibition can relax bronchi precontracted by histamine and neurokinin A, suggesting that RhoA activation is an integral part of the pathway leading to smooth muscle contraction.15 Methacholine and acetylcholine stimulate both muscarinic 3 (M3) and muscarinic 2 (M2) receptors.22,27 ...
Sigma-Aldrich offers abstracts and full-text articles by [Tamara Hilmenyuk, Iris Bellinghausen, Bärbel Heydenreich, Anne Ilchmann, Masako Toda, Stephan Grabbe, Joachim Saloga].
The Ova Antigen Delivery Reagent has been developed for efficient in vitro targeting of ovalbumin to antigen-presenting cells (APCs), for example, DCs. The reagent consists of a monoclonal anti-biotin antibody conjugated to ovalbumin and FITC. In combination with an appropriate biotinylated anti-receptor antibody, any desired antigen uptake receptor can be targeted.The Antigen Delivery Module Set comprises all the reagents that are required for the isolation of DCs, antigen delivery, and subsequent analysis of antigen presentation. - Österreich
This enhanced uptake of the intravenously administered antigen was partly retarded when OVA protein was intravenously injected into Col26-7ND tumorbearing WT
ml, 1 mg, 200 µl Ovalbumin Antibody (Monoclonal, 200 µL Ovalbumin Antibody (Monoclonal, 200 µl AFP Antibody (Monoclonal, 200 µL Aprotinin Antibody (Monoclonal, 200 µL CGRP Antibody (Monoclonal
Acute exposure of sensitized mice to antigen elicits allergic airway disease (AAD) characterized by Th2 cytokine-dependent pulmonary eosinophilia, methacholine hyperresponsiveness and antigen-specific IgE elevation. However, chronic exposure induces a local inhalational tolerance (LIT), with resolution of the airway responses but persistent systemic IgE production. To further determine if systemic immunologic responses were maintained during LIT, we assessed subcutaneous late phase responses to ovalbumin in this model. Sensitized and AAD mice developed small subcutaneous responses to ovalbumin, with footpad thickness increasing to 113.7 and 113.6% of baseline, respectively. In comparison, LIT mice developed marked foot swelling (141.6%). Histologic examination confirmed increased inflammation in the chronic animals, with a significant contribution by eosinophils. Thus, the resolution of airway inflammatory responses with chronic antigen inhalation is a localized response, not associated
The present study was carried out to verify if DMPP, a nAChR agonist, could have a protective effect on the development of airway inflammation and responsiveness in a murine model of asthma, and to evaluate possible mechanisms of action. The results support the hypothesis that these agonists have a protective effect on airway inflammation and responsiveness and that this effect may be related to antigen sensitisation and calcium metabolism. Taken together, these results confirm other published data by the current authors and others 8, 9, 10, 21.. It is important to separate the specific anti-inflammatory effects of nicotinic agonists, such as nicotine, from the overall immunosuppressive effect of smoking. The present authors do not wish to suggest that cigarette smoking could be beneficial for asthmatic patients. However, there is evidence in the literature that the use of nicotinic agonists could be efficient in treating some inflammatory diseases 8, 9, 10. As nicotine crosses the blood-brain ...
Background: The deleterious effects of atmospheric pollution on human health are also known by increasing incidence and exacerbations of pre-existing lung diseases such as asthma. However, the effects of diesel exhausted particles (DEP) on acute allergic pulmonary inflammation during asthma sensitization process remain unclear. Objective: To evaluate the effects of 5 weeks of DEP exposure during the sensitization process of acute allergic pulmonary inflammation in an experimental murine model. Methods: Male Swiss mice were divided into 4 groups: non OVA-sensitized and saline instillation animals (10 µL/mouse) (Control, n=6), non OVA-sensitized and DEP instillation mice (3 mg/mL-10 µL/mouse) (DEP, n=8), OVA-sensitized and saline instillation animals (OVA, n=6) and OVA-sensitized and DEP instillation mice (DEP+OVA, n=8). 24 hours after OVA or saline aerosol challenge anesthetized mice were euthanized and was performed measures of inflammatory cells from bronchoalveolar fluid (BALF) and IL-4, ...
Allergic throat hyperresponsiveness (AHR) in OVA-sensitized and challenged mice, mediated by allergen-specific Th2 cells and Th2-like iNKT cells, develops under the impact of inhibitory and enhancing Capital t cells. neglected contributor. Intra-peritoneal shot of Ovum/alum refurbished advancement of the AHR-enhancers in all of the mutant pressures, suggesting that the boosters can become caused when they fail to develop automatically still, and that they themselves want not really communicate TNFRp75, IL-4 or IFN- in purchase to exert their function. We consider that both the advancement and the cytokine potential of the AHR-enhancing Capital t cells differs vitally from that of Th2 cells and NKT cells, despite identical affects of these cell populations on AHR. can be not really very clear. Relating to one theory, fragile Ag-stimulation mementos ARRY-438162 Th2-reactivity. A Th2-prejudice also is present after delivery when the immune system program can be still premature (4). Nevertheless, it ...
Ovalbumin - Instruments Consumables Reagents Advanced BioMatrix,RANDOX,RANDOX ELISA,Biomedical, biochemical reagents, laboratory supplies, equipment, antibodies, ELISA kits, diagnostic reagents, methods of experimental techniques, general analytical instruments, material testing instruments and equipment, used laboratory equipment, instruments and equipment, life sciences, environmental monitoring equipment , measurement, measuring instruments, rotating wall bioreactor, three-dimensional tissue / stem cell culture system; microcapsule
Creative Biolabs provides Mouse anti-OVA T cell receptor (22.3), pCDTCR1 product for Biopharmaceutical research,preclinical and clinical trials.
TY - JOUR. T1 - Role of macrophage migration inhibitory factor in ovalbumin-induced airway inflammation in rats. AU - Kobayashi, M.. AU - Nasuhara, Y.. AU - Kamachi, A.. AU - Tanino, Y.. AU - Betsuyaku, Tomoko. AU - Yamaguchi, E.. AU - Nishihira, J.. AU - Nishimura, M.. PY - 2006/4. Y1 - 2006/4. N2 - Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine that reportedly counteracts the anti-inflammatory effect of endogenous glucocorticoids. There have only been a few reports that demonstrate a potential link between MIF and bronchial asthma. In an attempt to further clarify the precise role of MIF in asthma, the present authors examined the effect of anti-MIF antibody (Ab) on airway inflammation and airway hyperresponsiveness in an ovalbumin-immunised rat asthma model. Actively immunised Brown Norway rats received ovalbumin inhalation with or without treatment of anti-MIF Ab. The levels of MIF in bronchoalveolar lavage fluid were significantly elevated after the ovalbumin ...
Chicken Egg Ovalbumin (Gal d 2) ELISA Kit, 96 tests, Quantitative ELISA Kit 6050 Chicken Egg Ovalbumin ELISA Kit Enzyme-linked immunosorbent assay (ELISA Kit); enzyme immunoassay (EIA); animal (mouse;; rat;; human;; monkey etc) crossreactivity; serum plasma and biolgoical fluids; peptide;; proteins;; ELISA;; antibody;; Chicken proteins Allergy ovalbumin turkey egg white yolk ovalbumin ova peptides autoimmune autoantibodies contaminants food allergens vaccines Chicken Egg Ovalbumin (Gal d 2) ELISA Kit, 96 tests, Quantitative ELISA Kit 6050 Chicken Egg Ovalbumin ELISA Kit Enzyme-linked immunosorbent assay (ELISA Kit); enzyme immunoassay (EIA); animal (mouse;; rat;; human;; monkey etc) crossreactivity; serum plasma and biolgoical fluids; peptide;; proteins;; ELISA;; antibody;; Chicken proteins Allergy ovalbumin turkey egg white yolk ovalbumin ova peptides autoimmune autoantibodies contaminants food allergens vaccines
TY - JOUR. T1 - Aerosol-induced immunoglobulin (Ig)-E unresponsiveness to ovalbumin does not require CD8+ or T cell receptor (TCR)-γ/δ+ T cells or interferon (IFN)-γ in a murine model of allergen sensitization. AU - Seymour, Brian W P. AU - Gershwin, Laurel J. AU - Coffman, Robert L.. PY - 1998/3/2. Y1 - 1998/3/2. N2 - Mice expressed for 20 min daily to aerosolized ovalbumin (OVA) for 10 d at concentrations from 1 to 0.01% OVA made greatly reduced immunoglobulin (Ig)-E responses to subsequent immunogenic OVA challenges, given either intraperitoneally or aerosol. This IgE-specific unresponsiveness lasted for at least four months. However, these aerosol-treated mice were primed for larger OVA-specific IgG1 and IgG2a responses. The specific reduction in IgE responses was not due to preferential induction of a T helper (Th)-1 response as aerosol OVA-primed mice made greatly reduced Th2 and no detectable Th1 response after rechallenge in vitro. Consistent with this, the increase in circulating ...
p,Mice fed the standard diet showed the induction of serum OVA-specific IgE production following oral sensitization with OVA plus CT (,xref ref-type=fig rid=pone-0044338-g001,,bold,Figure 1B,/bold,,/xref,) as previously described ,xref ref-type=bibr rid=pone.0044338-Li1,[12],/xref,. In contrast, the mice fed a standard diet plus resveratrol showed a decrease in OVA-specific IgE production after oral sensitization (,xref ref-type=fig rid=pone-0044338-g001,,bold,Figure 1B,/bold,,/xref,). Consistent with the findings of the serum IgE levels, resveratrol-fed mice sensitized with OVA plus CT showed a smaller drop in the extent of rectal temperatures than the control mice sensitized with OVA plus CT, upon intraperitoneal OVA challenge (,xref ref-type=fig rid=pone-0044338-g001,,bold,Figure 1C,/bold,,/xref,). In addition, OVA-specific IL-13 and IFN-γ productions from splenocytes and mesenteric lymph nodes (MLN)-derived cells were decreased in resveratrol-fed mice in comparison to those ...
Dai, Yun-Lei, Gui-Lian Shao, Fang Wang (2015) Extract of Brassica rapa suppresses ovalbumin-induced asthma by down-regulating NF-kB and p38 MAPK activity. [Publication] Full text not available from this repository ...
M. Al-Laith; A. Weyer; N. Havet; C. Dumarey; B. Vargaftig; M. Bachelet, 1992: Passive sensitization of guinea-pig lung mast cells and alveolar macrophages with anti-ovalbumin IgG1 and IgG2 antibodies
Cross-priming is a critical component of T cell responses to cancers and viruses, and involves transfer of antigen from antigen donor cells to the antigen presenting cells. In spite of the centrality of antigen in this process, the influence of the quantity of antigen expressed by the antigen donor cell on the efficiency of cross-priming remains unexamined. Here, I describe the creation of a novel system where the model antigen ovalbumin is expressed in P815 (d haplotype) cells under the control of an inducible promoter, producing a large amount of antigen synthesis upon induction. However, even in the un-induced condition, a very low level of ovalbumin can be detected using sensitive methods to amplify the weak signal. I have used titrated quantities of uninduced and induced cells, expressing vastly different quantities of ovalbumin, and have monitored cross-priming of the endogenous anti-OVA CD8+ T cell response quantitatively in C57BL/6 mice (b haplotype), using in vivo cytolytic T lymphocyte assays.
Cardiac antigen-specific CD8+ T cells are involved in the autoimmune component of human myocarditis. Here, we studied the differentiation and migration of pathogenic CD8+ T cell effector cells in a new mouse model of autoimmune myocarditis. A transgenic mouse line was derived that expresses cardiac myocyte restricted membrane-bound ovalbumin (CMy-mOva). The endogenous adaptive immune system of CMy-mOva mice displays tolerance to ovalbumin. Adoptive transfer of naive CD8+ T cells from the ovalbumin-specific T cell receptor-transgenic (TCR-transgenic) OT-I strain induces myocarditis in CMy-mOva mice only after subsequent inoculation with ovalbumin-expressing vesicular stomatitis virus (VSV-Ova). OT-I effector T cells derived in vitro in the presence or absence of IL-12 were adoptively transferred into CMy-mOva mice, and the consequences were compared. Although IL-12 was not required for the generation of cytolytic and IFN-γ-producing effector T cells, only effectors primed in the presence of ...
Cardiac antigen-specific CD8+ T cells are involved in the autoimmune component of human myocarditis. Here, we studied the differentiation and migration of pathogenic CD8+ T cell effector cells in a new mouse model of autoimmune myocarditis. A transgenic mouse line was derived that expresses cardiac myocyte restricted membrane-bound ovalbumin (CMy-mOva). The endogenous adaptive immune system of CMy-mOva mice displays tolerance to ovalbumin. Adoptive transfer of naive CD8+ T cells from the ovalbumin-specific T cell receptor-transgenic (TCR-transgenic) OT-I strain induces myocarditis in CMy-mOva mice only after subsequent inoculation with ovalbumin-expressing vesicular stomatitis virus (VSV-Ova). OT-I effector T cells derived in vitro in the presence or absence of IL-12 were adoptively transferred into CMy-mOva mice, and the consequences were compared. Although IL-12 was not required for the generation of cytolytic and IFN-γ-producing effector T cells, only effectors primed in the presence of ...
Asthma is a disease characterized by spontaneous contraction of the airways in response to a wide variety of endogenous and exogenous stimuli. Many asthma models are used to mimic the human asthma model in the literature. In order to better understand the role of the cannabinoid (CB) 2 receptor in the ovalbumin (OVA)-induced asthma model, a combination of both selective CB2 agonist (AM1241) and antagonist (AM630) was used to improve inflammatory hypersensitivity and edema in rats. In the present study, it was found that OVA decreased body weight (p|0.05), increased lung weights (p|0.05), increased diastolic and systolic blood pressure (p|0.001), and caused to irregularity in pulmonary functions (p|0.001). Moreover, CB2 agonist was found not to reduce body weight, cause blood pressure and respiratory irregularities (p| 0.05). OVA led to increase in IgE, TNF-α, IL-4, MDA level (p|0.001) and total WBC count (p|0.05). CB2 treatment caused to reduce the number of total WBC and the level of total protein in
Effects of respiratory viral infection on airway epithelium include airway hyper-responsiveness and inflammation. Both features may contribute to the development of asthma. Excessive damage and loss of epithelial cells are characteristic in asthma and may result from viral infection. To investigate apoptosis in Adenoviral-infected Guinea pigs and determine the role of death receptor and ligand expression in the airway epithelial response to limit viral infection. Animal models included both an Acute and a Chronic Adeno-infection with ovalbumin-induced airway inflammation with/without corticosteroid treatment. Isolated airway epithelial cells were cultured to study viral production after infection under similar conditions. Immunohistochemistry, western blots and viral DNA detection were used to assess apoptosis, death receptor and TRAIL expression and viral release. In vivo and in vitro Adeno-infection demonstrated different apoptotic and death receptors (DR) 4 and 5 expression in response to
Interleukin (IL)-4-producing-CD8 (cytotoxic T cells, Tc) contribute to lung eosinophilia and airway hyper-responsiveness (AHR) to an antigen. CD4+CD25+ regulatory T cells (Tregs) attenuate airway inflammation and AHR. This study investigated whether Tregs decrease Tc2frequencies in ovalbumin (OVA)-induced asthma model of mice. Female C57BL/6 mice were sensitized with OVA intraperitoneally and challenged with OVA intranasally to induce allergic asthma model. Tregs were sorted by fluorescence activated cell sorting (FACS) and magnetic activated cell sorting (MACS) microbeads. OVA-sensitized mice were injected with Tregs or phosphate buffer saline (PBS) by tail vein ahead of the first challenge. Airway inflammation and airway hyper-responsiveness (AHR)were evaluated by histological analysis and invasive method, respectively. OVA-specific IgE and cytokine levels were detected by ELISA. Flow cytometry was used to detect the percentages of Tc1 and Tc2. Gata3 and T-bet mRNA was determined by ...
Toxoplasma gondii is an obligate intracellular pathogen that is able to survive in a range of hosts due to its repertoire of secreted effector proteins. Utilizing the type I and type II Δku80 strains we engineered a collection of targeted knockout strains that lack a ROP or GRA protein. We determined the virulence of each strain in mice and identified type I GRA12, ROP5, ROP18 and type II ROP5 as major virulence factors. Controlling a T. gondii infection requires a strong CD8+ T cell and IFN-γ host immune response, which is activated by MHC-I antigen presentation. We engineered each of our type I knockout strains to express the model antigen ovalbumin (OVA) and tested each for their ability to modify MHC-I antigen presentation. Infection of macrophages and dendritic cells with knockout strains that lacked type I GRA2, GRA12, ROP5 or ROP18 exhibited significantly higher levels of antigen presentation than the OVA-expressing parental Δku80 strain. The ability of ROP18 to block antigen ...
Ovalbumin and lysozyme have been reported to undergo a mixed association in solutions of low ionic strength. Osmotic pressure experiments were performed on ovalbumin and on lysozyme solutions in 0.06 M cacodylate buffer (I = 0.02, pH = 5.8) at 30 and
An integrated investigative team of environmental and biomedical scientists from Michigan State University (MSU) and the Southern California Particle Center and Supersite (SCPCS) will conduct atmospheric and toxicologic research at three sites in the Los Angeles Basin (LAB), each of which have distinct air-pollution profiles. One designated site in the LAB contains locally generated PM and gaseous co-pollutants (source emission site). Another site contains transported PM and gaseous co-pollutants (receptor site). The third site is geographically located between the source and the receptor sites. The team will use a recently built, state-of-the-art mobile air research laboratory (AirCARE 1), parked at the LAB sites, to conduct inhalation toxicology studies exposing laboratory rodents with and without pre-existing allergic airway disease (animal model of human asthma) to concentrated PM (CAPs) from the local environment. Brown Norway (BN) rats with and without allergic airway disease will be ...
The prevalence of food allergy is rising in the western world. Allergen restriction is the chosen treatment in this condition, but continuous ingestion of the antigen has shown positive results in clinical trials. In a previous study, we have shown several allergic and metabolic alterations after 7 days of ovalbumin (OVA) ingestion by sensitized mice. The aim of this study was to investigate whether prolonged ingestion of antigen by sensitized mice would reverse the metabolic consequences caused by experimental food allergy. For this, allergic and metabolic parameters were analysed after prolonged ingestion of an OVA diet by OVA-sensitized mice. As shown previously, after 7 days of OVA consumption, sensitized mice showed increased serum levels of anti-OVA immunoglobulin (Ig)E and IgG1, aversion to the antigen ingestion, marked body and adipose tissue weight loss, followed by adipose tissue inflammation and decreased serum levels of adipokines, glucose and triglycerides. However, after 14 days of ...
Recently, epigenetic-mediated mechanisms - which involve heritable changes in gene expression in the absence of alterations in DNA sequences - have been proposed as contributing to asthma. In this issue of the JCI, Hollingsworth and colleagues report on the effect of prenatal maternal dietary intake of methyl donors on the risk of allergic airway disease in offspring in mice and show that these effects involve epigenetic regulation (see the related article beginning on page 3462). Supplementation of the maternal diet with methyl donors was associated with greater airway allergic inflammation and IgE production in F1 and, to some extent, F2 progeny. Site-specific differences in DNA methylation and reduced transcriptional activity were detected. If these findings are confirmed, a new paradigm for asthma pathogenesis may be emerging. ...
TY - JOUR. T1 - Non-CpG Oligonucleotides Exert Adjuvant Effects by Enhancing Cognate B Cell-T Cell Interactions, Leading to B Cell Activation, Differentiation, and Isotype Switching. AU - Herbáth, Melinda. AU - Papp, Krisztián. AU - Erdei, A.. AU - Prechl, J.. PY - 2015. Y1 - 2015. N2 - Natural and synthetic nucleic acids are known to exert immunomodulatory properties. Notably, nucleic acids are known to modulate immune function via several different pathways and various cell types, necessitating a complex interpretation of their effects. In this study we set out to compare the effects of a CpG motif containing oligodeoxynucleotide (ODN) with those of a control and an inhibitory non-CpG ODN during cognate B cell-T cell interactions. We employed an antigen presentation system using splenocytes from TCR transgenic DO11.10 mice and the ovalbumin peptide recognized by the TCR as model antigen. We followed early activation events by measuring CD69 expression, late activation by MHC class II ...
Dose-dependent specific antibody production, antigen-dependent pulmonary inflammation, and thiol changes in the lung and associated lymph nodes were examined in a Brown Norway rat model of pulmonary sensitization. Cysteine (CYSH), glutathione (GSH), and markers of inflammation in bronchoalveolar lavage fluid (BALF) were measured following ovalbumin (OVA) inhalation challenge. Alveolar macrophages
MnTE-2-PyP, a superoxide dismutase mimetic, inhibited OVA-induced airway inflammation in mice suggesting an impact in Th2 responsiveness. on immature DC. Our research claim that the main mechanism where MnTE-2-PyP inhibits airway irritation is normally by functioning on the DC and suppressing Th2 cell proliferation and activation. allergen provocation is normally elevated in asthmatics [6]. Furthermore, asthmatic sufferers demonstrate depressed degrees of endogenous antioxidant immune system such as for example superoxide dismutase (SOD) and glutathione [7]. Our lab is rolling out a SOD mimetic, MnTE-2-PyP [chemical substance name: Manganese (III) with MnTE-2-PyP demonstrated a reduced capability to support T cell proliferation, recommending an inhibitory function of MnTE-2-PyP on APC function [17]. Tse < 0.01) separate of OVA323C339 peptide concentrations when the SOD mimetic was within the culture mass media. Amount 2 MnTE-2-PyP inhibits Th2 cell proliferation. Th2 and DC cells were ...
Grapefruit seed extract (GSE) is a liquid extracted from the seeds, pulp, and white membranes of grapefruit. The herb has been used in traditional medicine as an anti bacterial, viral, fungal, parasites and to enhance immune system, protect against colds and flu and malignant disease. Grape seed extract (GSPE) may alleviate the symptoms od asthma through its antioxidant activities. According to the study of University of Ulsan College of Medicine, GSPE effectively suppressed inflammation in both acute and chronic mouse models of asthma, through reduced airway hyperresponsiveness (AHR), decreased inflammatory cells in the BAL fluid, reduced lung inflammation and and decreased IL-4, IL-5, IL-13, and eotaxin-1 expression(14) by downregulating the iNOS expression(15). Other study suggested that GSE significantly ameliorated of the pathological changes of ovalbumin-induced asthma, through inhibition of altered the lung parenchyma pathology and inflammation in ovalbumin-induced murine asthma model(16). ...
Changes in the levels of transforming growth factor (TGF)-beta cytokines or receptors observed during the progression of several inflammatory and fibrotic disorders have been used to implicate these cytokines in the pathophysiology of these diseases. Although correlative, these studies were inconclusive because they were unable to demonstrate actual continuous TGF-beta-mediated signaling in the involved tissues. We reasoned that the phosphorylation state and subcellular localization of Smad2, the intracellular effector of TGF-beta/activin-mediated signaling, could be used as a marker of active signaling mediated by these cytokines in situ. We therefore used an experimental model of ovalbumin-induced allergic airway inflammation and were able to demonstrate a dramatic increase in the numbers of bronchial epithelial, alveolar, and infiltrating inflammatory cells expressing nuclear phosphorylated Smad2 within the allergen-challenged lungs. This was accompanied by strong upregulation of the activin ...
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Mouse Monoclonal Anti-Ovalbumin Antibody (6 F11) [Alexa Fluor® 647]. Validated: ELISA. Tested Reactivity: Chicken. 100% Guaranteed.
November 2014. Attenuation of allergic airways inflammation by an extract of Hymenocardia acida Tracheal hyperresponsiveness, airway mucus production and bronchoalveolar inflammation are the major components of asthma. Here, we aim to investigate the role in the control of asthma of a bioactive plant extracted from Hymenocardia acida in a physiological and pathophysiological model. The effect of H. acida crude extract (HACE) on total cellular components of bronchoalveolar (BAL) fluids was performed on ovalbumin.... Author(s): Fatou Bintou Sar, Mamadou Sarr, Mama S.Y. Diallo, Saliou Ngom, Lamine Gueye, Abdoulaye Samb, Ramaroson Andriantsitohaina and Annelise Lobstein ...
Principal Investigator:OBOKI Keisuke, Project Period (FY):2010 - 2011, Research Category:Grant-in-Aid for Young Scientists (B), Research Field:膠原病・アレルギー・感染症内科学
Thus we questioned whether IL-4-producing CD8 T cells would be induced in vivo in response to primary immunization with alum-precipitated protein. The approach has been to compare the polarization of transgenic naïve ovalbumin-specific CD4 (OTII) and CD8 (OTI) T cells during their response to alum-precipitated ovalbumin (alumOVA). By addressing this question we have obtained further insight into the way early Th2-features are acquired by CD4 T cells in vivo in response to alum-precipitated protein.. Although both CD4 and CD8 OVA-specific T cells proliferate (30) in response to alumOVA, the acquisition of Th2-features, such as IL-4 and IL-13 mRNA up-regulation, is exclusively confined to CD4 T cells (Fig. 2). In addition, we confirm that mRNA specific for IL-17RB is strongly induced in vivo in alumOVA-responding OTII cells, but not in OTI cells responding to the same antigen (Fig. 2).. Conclusions. These findings indicate that the induction of IL-17RB expression is a selective feature of CD4 T ...
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TxCell (Valbonne, France) aims to treat severe chronic inflammatory and autoimmune diseases by using Tregs to tamp down the immune response. TxCell is developing therapeutics based on isolating Tregs from a patients blood, expanding them in the lab in response to a specific activating antigen, and infusing them back into the patient. Although the process is expensive, the extracted material can be frozen and used as needed, providing enough material for five years worth of treatment every few months.. Their most advanced clinical-stage product is Ova-Treg, which is in development for Crohns disease. Tregs are exposed to the protein ovalbumin, which makes up about 60% of egg whites. The Tregs recognizing ovalbumin are expanded and infused back into the patients body. Tregs naturally migrate to sites of major inflammation-or in the case of Crohns disease, the gut. Although ovalbumin is not related to the pathology of Crohns, it is typically present in the gut, so the ovalbumin-sensitive ...
Clone REA1002 recognizes the ovalbumin-derived peptide SIINFEKL bound to the MHC class I allele H-2Kb. The Anti-H-2Kb/SIINFEKL antibody is used to investigate antigen processing pathways in vivo and in vitro . Additional information: Clone REA1002 displays negligible binding to Fc receptors. - USA
Nitration of the Egg-Allergen Ovalbumin Enhances Protein Allergenicity but Reduces the Risk for Oral Sensitization in a Murine Model of Food Allergy ...
Protein Digest Standards are an ideal tool for the standardization, calibration, and troubleshooting of chromatographic separations, particularly for LC-MS applications. Each Protein Digest Standard is provided as a lyophilized mixture of tryptically digested peptides.
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5T33-OVA phenotypic analysis after transduction by a lentiviral vector encoding cytoplasmic ovalbumin (A) 5T33 and 5T33-OVA staining with PE-conjugated antibody
How to prevent blighted ovum - What can me and my wife do or take to prevent blighted ovum? Nothing. A blighted ovum is a pregnancy that simply didn
摘要: 目的:探讨白芦藜醇对中性粒细胞性哮喘小鼠肺组织炎症的抑制作用,并阐明其作用机制。方法:选取18只C57BL/6J雌性小鼠,采用脂多糖(LPS)+卵白蛋白(OVA)联合致敏方法建立中性粒细胞性哮喘小鼠模型,随机分为模型组(LPS+OVA组)、白藜芦醇组(Res组,于激发前2 h灌胃30 mg·kg-1白藜芦醇)和N-乙酰半胱氨酸组(NAC组,于激发前2 h灌胃3 mmol·kg-1 ...
Imagine what it would be like to finally be able to conceive a baby. In this article we will go over your most fertile days to conceive and find out exactly what you need to do to get pregnant right now. You are much more likely to have a better cha... Read , ...
Ovalbumin is the protein which makes up around two-thirds of the white of an egg. When an egg is cooked, the ovalbumin changes ... Hu, H. Y.; Du, H. N. (2000). "α-to-β Structural transformation of ovalbumin: Heat and pH effects". Journal of Protein Chemistry ... Huntington, J. A.; Stein, P. E. (2001). "Structure and properties of ovalbumin". Journal of Chromatography B. 756 (1-2): 189- ...
Perlmann, G. E. (1948). "Electrophoretic behavior of modified ovalbumins". Nature. 161 (4097): 720-721. doi:10.1038/161720b0. ...
Turato, C.; Pontisso, P. (2015). "SERPINB3 (serpin peptidase inhibitor, clade B (ovalbumin), member 3)". Atlas of Genetics and ...
Two amino acid storage proteins in animals are casein and ovalbumin. Seeds, particularly of leguminous plants, contain high ...
... and ovalbumin from egg white". Poultry Science. 93 (4): 1001-1009. doi:10.3382/ps.2013-03403. PMID 24706978. Fee, Conan J.; Van ...
The most common carriers include serum globulin, albumins, ovalbumin and many others. Although proteins are mostly employed for ...
After their discovery in adenovirus, introns were found in a number of eukaryotic genes such as the eukaryotic ovalbumin gene ( ... Lai EC, Woo SL, Dugaiczyk A, Catterall JF, O'Malley BW (May 1978). "The ovalbumin gene: structural sequences in native chicken ... O'Hare K, Breathnach R, Benoist C, Chambon P (September 1979). "No more than seven interruptions in the ovalbumin gene: ...
Parlak, A; Yildirim, S; Bagcivan, I; Durmus, N (October 2012). "Role of New Agents Affecting NO/cGMP Pathway on Ovalbumin- ...
FICZ likewise suppressed pulmonary Th2-type cytokine production in a mouse model of ovalbumin-induced allergic asthma. Dynamic ... In vitro, FICZ markedly inhibited the lipopolysaccharide- and ovalbumin-induced proliferation of T cells. ... suppresses pulmonary eosinophilia and Th2-type cytokine production in a mouse model of ovalbumin-induced allergic asthma". ...
More recently he has developed faster methods for RNA sequencing and has applied them to transfer RNAs and ovalbumin messenger ... He also studied the DNA sequence of the ovalbumin gene and its insertion sequences. He determined the nucleotide sequence of ...
Ovalbumin (OVA) Also known as egg albumin, OVA is the main protein (60-75%) found in hen egg white. OVA is soluble in Dimethyl ...
"Therapeutic Potential of Morin in Ovalbumin-induced Allergic Asthma Via Modulation of SUMF2/IL-13 and BLT2/NF-kB Signaling ...
... ovalbumin (Gal d 2), ovotransferrin (Gal d 3) and lysozyme (Gal d 4). Of these, ovomucoid is the dominant allergen, and one ... Mayonnaise Meringue or meringue powder Ovalbumin (egg white protein) Ovoglobulin (egg white protein) Ovomucin (egg white ...
Three-component protein array containing the proteins human fibrinogen, ovalbumin, and bovine IgG is shown in Figure 17, SPR ...
Prior to 1984, partially purified proteins such as bovine serum albumin, ovalbumin, or gelatin from various species had been ...
... member states of the European Union must also disclose after 30 June 2012 that the wine was treated with casein and ovalbumin, ...
Piedmontese for the commune of Ovada Ovalbumin (OVA), a protein found in egg whites .ova, filename extension in Open ...
Studies have shown that vaccines encoded with the chicken antigen, ovalbumin (OVA), in conjunction with cGAMP, were able to ...
... could function as a vaccine adjuvant when immunizing mice with hepatitis B surface antigen and soluble ovalbumin. An April 2002 ...
... ovalbumin and bovine serum albumin) with molecular masses of 14-66 ku can be recovered with an average yield of about 73,6%. ...
... and many of its proteins and blood vessels The albumin is made up of globular proteins called ovalbumin which contain over half ...
In a mice model of ovalbumin-induced allergic airway disease, 12-HHT and its companion cyclooxygenase metabolites, ... showed a statistically significantly increase in bronchoalveolar lavage fluid levels after intratracheal ovalbumin challenge; ...
Amyloid form of ovalbumin evokes native antigen-specific immune response in the host: prospective immuno-prophylactic potential ...
... and β-N-acetyl-glucosamine ovalbumin and showing that only the second reacted with group A antisera. A similar analytical ...
The function of ovalbumin is unknown, although it is presumed to be a storage protein. Ovalbumin is an important protein in ... Ovalbumin's signal sequence is not cleaved off, but remains as part of the mature protein. When heated, ovalbumin undergoes a ... Ovalbumin (abbreviated OVA) is the main protein found in egg white, making up approximately 55% of the total protein. Ovalbumin ... Ovalbumin also has several modifications, including N-terminal acetylation (G1), phosphorylation (S68, S344), and glycosylation ...
PAI-2 is genetically similar to chicken ovalbumin (Gallus gallus), and is a close mammalian homolog.[9] Both ovalbumin and PAI- ... the ovalbumin family of serine protease inhibitors". Journal of Molecular Biology. 335 (2): 437-53. doi:10.1016/j.jmb.2003.10. ... The nearest mammalian homologue of chicken ovalbumin". The Journal of Biological Chemistry. 264 (10): 5495-502. PMID 2494165.. ... The nearest mammalian homologue of chicken ovalbumin". The Journal of Biological Chemistry. 264 (10): 5495-502. PMID 2494165.. ...
Also, ovalbumin is frequently contaminated with endotoxins. Ovalbumin is one of the extensively studied proteins in animal ... models and also an established model allergen for airway hyper-responsiveness (AHR). Commercially available ovalbumin that is ...
Ovalbumin is the most abundant protein in albumen. Classed as phosphoglycoprotein, during storage, it converts into s-ovalbumin ... Ovalbumin in solution is heat-resistant. Denaturation temperature is around 84°C, but it can be easily denatured by physical ... and trivalent metal cations into a complex and is more heat sensitive than ovalbumin. At its isoelectric pH (6.5), it can bind ...
Kandhare, A. D., Liu, Z., Mukherjee, A. A., & Bodhankar, S. L. (2019). Therapeutic potential of Morin in Ovalbumin-induced ...
... Stein P.E., Leslie A.G.W., Finch J.T., Carrell R.W. ... a proteolytically cleaved form of ovalbumin, as a starting model. The final refined model, including four ovalbumin molecules, ... Ovalbumin, the major protein in avian egg-white, is a non-inhibitory member of the serine protease inhibitor (serpin) ... The crystal structure of uncleaved, hen ovalbumin was solved by the molecular replacement method using the structure of ...
Samples of lyophilized ovalbumin were irradiated, under vacuum in a high energy, uniform field of γ-rays, to absorb dosages of ... Effects of γ-Rays on the Serologic Properties of Ovalbumin Message Subject (Your Name) has forwarded a page to you from The ... Effects of γ-Rays on the Serologic Properties of Ovalbumin. II. Fractions from Irradiated Lyophilized Protein. Charles A. Leone ... The serologic data are interpreted as revealing, in γ-irradiated lyophilized ovalbumin, the existence of a spectrum of ...
200 µL Ovalbumin Antibody (Monoclonal, 200 µl AFP Antibody (Monoclonal, 200 µL Aprotinin Antibody (Monoclonal, 200 µL CGRP ... ml, 1 mg, 200 µl Ovalbumin Antibody (Monoclonal. View topsellers by: Popularity Favorites Price Brand Title ... 200 µL Ovalbumin Antibody (Monoclonal, Hecs 0,6 E9) 1 mg/ml - $550.00 USD. ...
Mice were injected intraperitoneally with 0.1 ml (10 μg) ovalbumin complexed with 2 mg Al(OH)3 as previously described.19 After ... Methods: Baseline lung resistance and methacholine induced AHR were measured in mice sensitised to ovalbumin (OVA) and also in ... Suppression of airway hyperresponsiveness induced by ovalbumin sensitisation and RSV infection with Y-27632, a Rho kinase ... Suppression of airway hyperresponsiveness induced by ovalbumin sensitisation and RSV infection with Y-27632, a Rho kinase ...
Since ovalbumin mRNA has a complexity of 1890 nucleotides, the resulting DNA was more than twice the length of ovalbumin mRNA ... Purified ovalbumin messenger RNA was employed to selectively enrich the concentration of the gene coding for ovalbumin from ... The coding strand of the ovalbumin gene was partially purified from sheared chick DNA by affinity column chromatography using ... The concentrations of the ovalbumin DNA sequence in various DNA fractions were quantitated by measuring their rates of ...
Zosky, G. R., Larcombe, A. N., White, O. J., Burchell, J. T., Janosi, T. Z., Hantos, Z., ... Turner, D. J. (2008). Ovalbumin- ... Zosky, GR, Larcombe, AN, White, OJ, Burchell, JT, Janosi, TZ, Hantos, Z, Holt, PG, Sly, PD & Turner, DJ 2008, Ovalbumin- ... T1 - Ovalbumin-sensitized mice are good models for airway hyperresponsiveness but not acute physiological responses to allergen ... Ovalbumin-sensitized mice are good models for airway hyperresponsiveness but not acute physiological responses to allergen ...
Radioactive protein contained in this peak was precipitated by ovalbumin antiserum, co electrophoresed with ovalbumin on sodium ... Radioactive protein contained in this peak was precipitated by ovalbumin antiserum, co electrophoresed with ovalbumin on sodium ... Radioactive protein contained in this peak was precipitated by ovalbumin antiserum, co electrophoresed with ovalbumin on sodium ... Radioactive protein contained in this peak was precipitated by ovalbumin antiserum, co electrophoresed with ovalbumin on sodium ...
Th1, Th2, and Th0 CD4+ lines expressing identical ovalbumin (OVA)-specific T-cell receptors (TCRs) were generated from OVA- ... Th1, Th2, and Th0 lines were generated from ovalbumin (OVA)-specific T-cell receptor (TCR) transgenic mice and transferred into ...
... - Instruments Consumables Reagents Advanced BioMatrix,RANDOX,RANDOX ELISA,Biomedical, biochemical reagents, ... Worthington Ovalbumin, Low Endo™, Purified. EA. 100 mg. $819.00. WLS003059-10MG. Worthington Ovalbumin, Low Endo™, Purified. EA ... Worthington Ovalbumin, Low Endo™, Purified. EA. 500 mg. $2,215.20. WLS003062-500MG. Worthington Ovalbumin, Low Endo™, Purified ...
Early life Ovalbumin sensitization and aerosol challenge for the induction of allergic airway inflammation in a BALB/c murine ... 2019) Early life Ovalbumin sensitization and aerosol challenge for the induction of allergic airway inflammation in a BALB/c ... To address this issue, this protocol adapted an experimental animal model of disease for sensitization to ovalbumin during the ...
F232-IgE Ovalbumin. 7556-4. 602791. F232-IgE Ovalbumin. kU/L. 7556-4. ...
... nounThe albumin of egg white.Origin of ovalbumin Alteration of Late Latin ōvī albūmen Latin ōvī genitive sing. of ōvum egg ; ... Origin of ovalbumin. Alteration of Late Latin ōvī albūmen Latin ōvī genitive sing. of ōvum egg ; see ovum . Latin albūmen white ... "ovalbumin." YourDictionary, n.d. Web. 11 December 2018. ,https://www.yourdictionary.com/ovalbumin,. ... ovalbumin. (n.d.). Retrieved December 11th, 2018, from https://www.yourdictionary.com/ovalbumin ...
Vitexin inhibits inflammation in murine ovalbumin-induced allergic asthma.. Venturini CL1, Macho A1, Arunachalam K1, de Almeida ... The aim of the present work was to evaluate the anti-hypersensitive activity of vitexin in a murine ovalbumin (OVA)-induced ...
Ovalbumin Induced Allergic Rhinitis and Development of Prediabetes to Rats: Possible Role of Th2 Cytokines ... In present study we tried to understand the diabetogenic changes on the background of ovalbumin induced allergic rhinitis (OVA ...
Mouse monoclonal Ovalbumin antibody [8C6]. Validated in ELISA and tested in Chicken. Cited in 1 publication(s). Immunogen ... Shows high affinity for Ovalbumin in its native form. It does not recognize denatured Ovalbumin. ... Primary - Mouse Anti-Ovalbumin antibody [8C6] (ab17292) ELISA Secondary - Goat Anti-Mouse IgG H&L (HRP) (ab205719) WB, IP, ... Ab17290 and ab17292 are specific for native ovalbumin. Tested in inhibition ELISA, the two antibodies do not react with the ...
Osmotic pressure experiments were performed on ovalbumin and on lysozyme solutions in 0.06 M cacodylate buffer (I = 0.02, pH = ... Ovalbumin and lysozyme have been reported to undergo a mixed association in solutions of low ionic strength. ... Ovalbumin and lysozyme have been reported to undergo a mixed association in solutions of low ionic strength. Osmotic pressure ... 0/Membranes, Artificial; 9006-59-1/Ovalbumin; EC 3.2.1.17/Muramidase From MEDLINE®/PubMed®, a database of the U.S. National ...
Sesame Oil Attenuates Ovalbumin-Induced Pulmonary Edema and Bronchial Neutrophilic Inflammation in Mice. Dur-Zong Hsu,1 Chuan- ... Allergic airway inflammation was induced by sensitizing with two doses of 10 mg ovalbumin (OVA) and then challenged with 1% OVA ... M. W. Foster, Z. Yang, E. N. Potts et al., "S-nitrosoglutathione supplementation to ovalbumin-sensitized and -challenged mice ... To induce allergic airway inflammation, mice were sensitized with an intraperitoneal injection of 10 mg ovalbumin (OVA, albumin ...
Sulfhydryl-reactive ovalbumin (OVA) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated ... Thermo Scientific™ Imject Maleimide-Activated Ovalbumin is sulfhydryl-reactive ovalbumin (OVA) for hapten-carrier conjugation ... Maleimide-Activated Ovalbumin, supplied lyophilized in PBS (pH 7.2) with stabilizer. *Activated with 5 to 15 moles of maleimide ... Purified, carrier-grade ovalbumin has been modified with SMCC crosslinker to attach maleimide groups capable of forming ...
"Ovalbumin" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... This graph shows the total number of publications written about "Ovalbumin" by people in Harvard Catalyst Profiles by year, and ... A Chlamydia trachomatis Strain Expressing Ovalbumin Stimulates an Antigen-Specific CD4+ T Cell Response in Mice. Infect Immun. ... Below are the most recent publications written about "Ovalbumin" by people in Profiles. ...
Mice were sensitized with s.c. injection of ovalbumin (OVA) on days 1 and 8. Sensitized mice received on days 9 and 12 on the ... Mice were sensitized with s.c. injection of ovalbumin (OVA) on days 1 and 8. Sensitized mice received on days 9 and 12 on the ... Mice were sensitized with s.c. injection of ovalbumin (OVA) on days 1 and 8. Sensitized mice received on days 9 and 12 on the ... Ovalbumin-sensitized mice were sacrificed on day 15 and 22 by cervical dislocation, exsanguinated, and lungs were removed. Main ...
... were measured following ovalbumin (OVA) inhalation challenge. Alveolar macrophages ... Dose-dependent thiol and immune responses to ovalbumin challenge in Brown Norway rats.. ... were measured following ovalbumin (OVA) inhalation challenge. Alveolar macrophages (AM) and pulmonary-associated lymph node ...
Order monoclonal and polyclonal Ovalbumin antibodies for many applications. Selected quality suppliers for anti-Ovalbumin ... ovalbumin) , egg albumin , member 14 , ovalbumin , plakalbumin , serpin peptidase inhibitor , serine (or cysteine) proteinase ... anti-Ovalbumin antibody (OVA) (AA 1-350) , Synonym: serine (or cysteine) peptidase inhibitor, clade B, member 1, pseudogene ... anti-Ovalbumin antibody (OVA) (HRP) , Synonym: serine (or cysteine) peptidase inhibitor, clade B, member 1, pseudogene Rabbit, ...
The orange fluorescent Alexa Fluor 555 ovalbumin conjugate is a protein with a relatively low molecular weight (~45,000 daltons ... The orange fluorescent Alexa Fluor® 555 ovalbumin conjugate is a protein with a relatively low molecular weight (~45,000 ...
In this study, we investigated the effect of topical application of KM110329 ethanol extract on the ovalbumin (OVA) or 2,4- ... In this study, we investigated the effect of topical application of KM110329 ethanol extract on the ovalbumin (OVA) or 2,4- ... Topical Application of Herbal Mixture Extract Inhibits Ovalbumin- or 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis,. ... Topical Application of Herbal Mixture Extract Inhibits Ovalbumin- or 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis. ...
... Public Deposited Citeable URL:. http://ir.library.oregonstate.edu/concern/ ...
Sample records for OVALBUMINE (ovalbumin). from WorldWideScience.org. Sample records 1 - 1 shown. ...
... sensitization and aerosol challenge with ovalbumin (OVA). DC, eosinophils, macrophages, T cells and B cells in lung and trachea ... Pulmonary dendritic cell distribution and prevalence in guinea pig airways: effect of ovalbumin sensitization and challenge.. ... sensitization and aerosol challenge with ovalbumin (OVA). DC, eosinophils, macrophages, T cells and B cells in lung and trachea ...
The National Center for Biomedical Ontology was founded as one of the National Centers for Biomedical Computing, supported by the NHGRI, the NHLBI, and the NIH Common Fund under grant U54-HG004028 ...
Lyophilized ovalbumin was irradiated by means of γ-rays over a wide range of dosages. From measurements of the displacement of ... Effects of γ-Rays on the Serologic Properties of Ovalbumin Message Subject (Your Name) has forwarded a page to you from The ... Effects of γ-Rays on the Serologic Properties of Ovalbumin. I. Irradiated Lyophilized Protein. Charles A. Leone ... the precipitin curve of irradiated ovalbumins from the position of the curve for native ovalbumin the loss of activity due to ...
We revealed that intratracheal chronic ovalbumin (OVA) exposure induces colitis and allergic airway inflammation. Interestingly ... We revealed that intratracheal chronic ovalbumin (OVA) exposure induces colitis and allergic airway inflammation. Interestingly ... Intratracheal Ovalbumin Administration Induces Colitis Through the IFN-γ Pathway in Mice. Kyoung-Hwa Jung1†, Dasom Shin1†, ... Keywords: allergy and immunology, colitis, IFN-γ, ovalbumin, Th1 cells. Citation: Jung K-H, Shin D, Kim S, Min D, Kim W, Kim J ...
The denatured state of ovalbumin. F Ahmad, A Salahuddin. Biochemical Journal Jun 01, 1972, 128 (1) 49P; DOI: 10.1042/ ...
... ovalbumin, ovalbumin-related protein Y (OVAY), and ovalbumin-related protein X (OVAX). Ovalbumin is the major egg white protein ... Similar to ovalbumin and OVAY, OVAX belongs to the ovalbumin serine protease inhibitor family (ov-serpin). We show that OVAX is ... as opposed to ovalbumin. Interestingly, OVAX, unlike ovalbumin, displays antibacterial activities against both Listeria ... Ovalbumin-related protein X is a heparin-binding ov-serpin exhibiting antimicrobial activities.. [Sophie Réhault-Godbert, ...
Prolonged ingestion of ovalbumin diet by sensitized mice improves the metabolic consequences induced by experimental food ... In a previous study, we have shown several allergic and metabolic alterations after 7 days of ovalbumin (OVA) ingestion by ...
Chicken ovalbumin upstream promoter transcription factor II regulates uncoupling protein 3 gene transcription in Phodopus ... Chicken ovalbumin upstream promoter transcription factor II regulates uncoupling protein 3 gene transcription in Phodopus ... Chicken ovalbumin upstream promoter transcription factor II regulates uncoupling protein 3 gene transcription in Phodopus ... Chicken ovalbumin upstream promoter transcription factor II regulates uncoupling protein 3 gene transcription in Phodopus ...
The National Center for Biomedical Ontology was founded as one of the National Centers for Biomedical Computing, supported by the NHGRI, the NHLBI, and the NIH Common Fund under grant U54-HG004028 ...
Mouse Monoclonal Anti-Ovalbumin Antibody (6 F11) [Alexa Fluor® 405]. Validated: ELISA. Tested Reactivity: Chicken. 100% ... Ovalbumin Antibody (6 F11) [Alexa Fluor® 405] Summary. Immunogen. Purified chicken ovalbumin adsorbed onto aluminum hydroxide ... Blogs on Ovalbumin. There are no specific blogs for Ovalbumin, but you can read our latest blog posts. ... Reviews for Ovalbumin Antibody (NBP1-05177AF405) (0) There are no reviews for Ovalbumin Antibody (NBP1-05177AF405). By ...
Groups of BALB/c mice were injected intraperitoneally with ovalbumin, ovalbumin + alum, ovalbumin + uric acid, uric acid, alum ... ovalbumin allopurinol - untreated groups. Groups that received alum, alum + ovalbumin, uric acid, and uric acid + ovalbumin had ... The groups that received alum + ovalbumin or uric acid + ovalbumin had the highest numbers of IL-4 and INF-γ secreting cells as ... Allopurinol administration one day prior to alum + ovalbumin or uric acid + ovalbumin resulted in a decrease in the number of ...
Transient Receptor Potential Ankyrin 1 Enhances Ovalbumin-Induced Acute Allergic Inflammation in Murine Models.. 07:00 EST 30th ... Summary of "Transient Receptor Potential Ankyrin 1 Enhances Ovalbumin-Induced Acute Allergic Inflammation in Murine Models.". ... Home » Citations » Transient Receptor Potential Ankyrin 1 Enhances Ovalbumin-Induced Acute Allergic Inflammation in Murine ...
... chicken ovalbumin upstream promoter-transcription factor; chicken ovalbumin upstream promoter-transcription factor I; couptf6; ... chicken ovalbumin upstream promoter 1, v-erb-a homolog-like 3); unc-55; wu:fc10d05; zCOUP-TFI; zSvp[44] ...
  • Ovalbumin, the major protein in avian egg-white, is a non-inhibitory member of the serine protease inhibitor (serpin) superfamily. (uniprot.org)
  • Radioactive protein contained in this peak was precipitated by ovalbumin antiserum, co electrophoresed with ovalbumin on sodium dodecyl sulfate (SDS) and urea gels at pH 8.7, and eluted with the protein at the same pH (4.8) on CM cellulose chromatography. (elsevier.com)
  • Baseline lung resistance and methacholine induced AHR were measured in mice sensitised to ovalbumin (OVA) and also in mice infected with respiratory syncytial virus (RSV) following ovalbumin sensitisation (OVA/RSV). (bmj.com)
  • Methods: BALB/c mice were sensitized to ovalbumin (OVA) and challenged with one or three OVA aerosols. (elsevier.com)
  • Turner, D. J. / Ovalbumin-sensitized mice are good models for airway hyperresponsiveness but not acute physiological responses to allergen inhalation . (elsevier.com)
  • Th1, Th2, and Th0 lines were generated from ovalbumin (OVA)-specific T-cell receptor (TCR) transgenic mice and transferred into lymphocyte-deficient, OVA-treated severe combined immunodeficiency (SCID) mice. (jci.org)
  • The coding strand of the ovalbumin gene was partially purified from sheared chick DNA by affinity column chromatography using ovalbumin mRNA immobilized on phosphocellulose. (scripps.edu)
  • The concentrations of the ovalbumin DNA sequence in various DNA fractions were quantitated by measuring their rates of hybridization with 125I-labeled ovalbumin mRNA. (scripps.edu)
  • When apparent Cot1/2 values of these reactions were compared to the apparent Cot1/2 value obtained from the hybridization reaction between 125I-ovalbumin mRNA and complementary DNA synthesized against ovalbumin mRNA using the enzyme reverse transcriptase, purification of the coding ovalbumin DNA strand over total chick DNA was estimated to be approximately 9,600-fold. (scripps.edu)
  • Since ovalbumin mRNA has a complexity of 1890 nucleotides, the resulting DNA was more than twice the length of ovalbumin mRNA and thus should contain DNA sequences adjacent to the structural portion of the ovalbumin gene. (scripps.edu)
  • Translation of ovalbumin mRNA in Xenopus laevis oocytes. (elsevier.com)
  • Ovalbumin messenger RNA (mRNA(ov)) purified from hen oviduct was injected into Xenopus laevis oocytes. (elsevier.com)
  • Injection of increasing amounts of mRNA(ov) was found to elicit a linear response in terms of ovalbumin synthesis. (elsevier.com)
  • Ovalbumin mRNA activity was present in RNA preparations from chicks treated with estrogen but was undetectable in animals withdrawn from the hormone. (elsevier.com)
  • In this study, we used an ovalbumin (OVA)-induced, CD103-knockout (KO) BALB/c mouse model of experimental allergic airways disease (EAAD) to investigate the role of CD103 in disease expression, CD4+ T-cell activation and DC activation and function in airways and lymph nodes. (edu.au)
  • The ovalbumin gene. (scripps.edu)
  • Purified ovalbumin messenger RNA was employed to selectively enrich the concentration of the gene coding for ovalbumin from total chick DNA by molecular hybridization. (scripps.edu)
  • To address this issue, this protocol adapted an experimental animal model of disease for sensitization to ovalbumin during the immediate post-weaning period beginning at 21 days of age. (edu.au)
  • The final refined model, including four ovalbumin molecules, 678 water molecules and a single metal ion, has a crystallographic R-factor of 17.4% for all reflections between 6.0 and 1.95 A resolution. (uniprot.org)
  • The crystal structure of uncleaved, hen ovalbumin was solved by the molecular replacement method using the structure of plakalbumin, a proteolytically cleaved form of ovalbumin, as a starting model. (uniprot.org)
  • Youssef M, De Sanctis JB, Kanagaratham C, Tao S, Ahmed E, Radzioch D. Efficacy of Optimized Treatment Protocol Using LAU-7b Formulation against Ovalbumin (OVA) and House Dust Mite (HDM) -Induced Allergic Asthma in Atopic Hyperresponsive A/J Mice. (harvard.edu)
  • A Chlamydia trachomatis Strain Expressing Ovalbumin Stimulates an Antigen-Specific CD4+ T Cell Response in Mice. (harvard.edu)
  • Mice were sensitized with s.c. injection of ovalbumin (OVA) on days 1 and 8. (frontiersin.org)
  • In a previous study, we have shown several allergic and metabolic alterations after 7 days of ovalbumin (OVA) ingestion by sensitized mice. (wiley.com)
  • Splenocytes from mice sensitized with ovalbumin (OVA) were isolated for analyses of cytokine production and cell viability. (mdpi.com)
  • Alternaria extract, when administered to mice intranasally with ovalbumin (OVA) protein, induces a rapid (1-48 h) innate response while also priming an OVA-specific Th2 response that can be evoked 14 days later by intranasal administration of OVA alone. (nih.gov)
  • Shibayagi's OVA-IgE ELISA KIT is a useful tool for studying mouse immune system by measuring specific anti-OVA IgE after immunization of mice with OVA (ovalbumin). (biovendor.com)
  • Intradermal immunization with ovalbumin-loaded poly-epsilon-caprolactone microparticles conferred protection in ovalbumin-sensitized allergic mice. (cun.es)
  • Baseline lung resistance and methacholine induced AHR were measured in mice sensitised to ovalbumin (OVA) and also in mice infected with respiratory syncytial virus (RSV) following ovalbumin sensitisation (OVA/RSV). (bmj.com)
  • Gloriosaol isomeric mixtures (GLM) are isolated from the extract, structurally confirmed FIA-MS/MS. Both YGE and GLM are evaluated against ovalbumin-induced airway hyperresponsiveness (AHR) in Balb/C mice. (omicsonline.org)
  • In this study, we investigated the acute oral toxicity of an Insampaedok-san water extract (ISSE) in rats and the antiasthmatic effects of ISSE and its mechanism in a model of asthma induced by ovalbumin (OVA) in mice. (biomedcentral.com)
  • The plasticizer di-(2-ethylhexyl) phthalate (DEHP) has been shown to stimulate a non-allergy related immune response with increased levels of IgG1 and IgG2a, but not IgE, after co-administration with the model allergen ovalbumin (OVA) in mice. (biomedcentral.com)
  • IgE, IgG and lymph node T cell responses in mice were studied using ovalbumin mixed with topical transdermal cream formulations containing a potential immune modulator applied to the skin. (longdom.org)
  • BALB/c mice were immunized with Ovalbumin (OVA) mixed with alum to induce an IgE response. (longdom.org)
  • Mice developed sIgE to ovalbumin after 14 days and application of cream formulations showed a dose dependent reduction in IgE compared to controls at 42 and 70 days. (longdom.org)
  • Therefore, it was hypothesized that the topical application of an allergen contained in a transdermal base cream would produce Anti-Ovalbumin (OVA) antibody production in mice. (longdom.org)
  • All mice received a single injection of 2 nanograms (ng) chicken Ovalbumin (OVA) mixed with alum intraperitoneally (i.p.) on day 0 in order to stimulate an immune IgG and IgE response. (longdom.org)
  • Therefore, deregulation of miRNAs was assessed in whole lungs from mice with ovalbumin (OVA)-induced allergic airway inflammation (AAI). (nature.com)
  • Female 6-8 week old Balb/c mice were intraperitoneally (i.p.) sensitized five times with ovalbumin (OVA) (d7, d14, d28, d42 and d56) (Sigma Aldrich, St. Louis, USA) or phosphate buffered saline (PBS) and challenged two times (d70 and d71) with 1% OVA aerosol to induce allergic airway inflammation. (nature.com)
  • Cytotoxic T lymphocytes (CTL) from the ovalbumin-hsp70-immunized mice and a highly effective CTL clone (4G3) raised against ovalbumin-expressing EL4 tumor cells (EG7-OVA) were equally effective in terms of the concentration of this compound required for half-maximal lysis in a CTL assay. (bachem.com)
  • Ovalbumin-treated mice were used as an asthma model. (elsevier.com)
  • FL- or GM-CSF-treated mice injected with soluble ovalbumin display dramatic increases in antigen-specific antibody titers, but the isotype profiles seem critically dependent on the cytokine used. (pnas.org)
  • Prepare hapten-carrier conjugates to generate antibodies against cysteine-containing peptides and other thiolated antigens using this sulfhydryl-reactive ovalbumin protein. (fishersci.ca)
  • Levels of IgG and IgA antibodies to ovalbumin did not differ between diabetic and control children. (diabetesjournals.org)
  • The levels of cholera toxin and ovalbumin IgG antibodies secreted in serum and ileal and reproductive tract secretions were determined by ELISA. (lincoln.ac.nz)
  • However, only one animal produced ovalbumin antibodies in serum. (lincoln.ac.nz)
  • Cholera toxin and ovalbumin antibodies were significantly increased in vaginal secretions at week 7 as compared to pre-immunisation levels. (lincoln.ac.nz)
  • The direct immunisation of cholera toxin and ovalbumin into ileal lumen, significantly increased cholera toxin antibody levels in serum and reproductive tract secretions but it did not reliably increase ovalbumin antibodies in serum or mucosal secretions which suggests that cholera toxin was not an effective adjuvant to potentiate the serum immune response to ovalbumin in this experiment. (lincoln.ac.nz)
  • In general, production of specific antibodies to ovalbumin and cholera toxin in the reproductive tract secretion and serum following immunisation into the gut indicates that the possum possesses a common mucosal immune system. (lincoln.ac.nz)
  • Ovalbumin (257-264) is an antigenic peptide fragment of ovalbumin that contains a CD8+ cytotoxic T-cell (CTL)-specific epitope with the sequence 'SIINFEKL. (lktlabs.com)
  • Dissection of major histocompatibility complex (MHC) and T cell receptor contact residues in a Kb-restricted ovalbumin peptide and an assessment of the predictive power of MHC-binding motifs. (lktlabs.com)
  • SIINFEKL, an ovalbumin-derived peptide, is known to arise from natural processing of ovalbumin H-2b mouse cells. (bachem.com)
  • There are currently no images for Ovalbumin Antibody (NBP1-05177AF405). (novusbio.com)
  • Also, cholera toxin and ovalbumin antibody levels in vaginal secretions were significantly higher than ileal and other reproductive tract secretions. (lincoln.ac.nz)
  • In an attempt to further clarify the precise role of MIF in asthma, the present authors examined the effect of anti-MIF antibody (Ab) on airway inflammation and airway hyperresponsiveness in an ovalbumin-immunised rat asthma model. (elsevier.com)
  • Vitexin inhibits inflammation in murine ovalbumin-induced allergic asthma. (nih.gov)
  • Allergic airway inflammation was induced by sensitizing with two doses of 10 mg ovalbumin (OVA) and then challenged with 1% OVA nebulizer exposure (1 h/day) for 3 days. (hindawi.com)
  • Cysteine (CYSH), glutathione (GSH), and markers of inflammation in bronchoalveolar lavage fluid (BALF) were measured following ovalbumin (OVA) inhalation challenge. (cdc.gov)
  • We revealed that intratracheal chronic ovalbumin (OVA) exposure induces colitis and allergic airway inflammation. (frontiersin.org)
  • To reveal the mechanisms underlying the effects of allergic airway inflammation on IBD pathogenesis, we hypothesized that intratracheal ovalbumin (OVA) exposure would induce inflammation in both the lung and the colon, as it is well-known that OVA exposure causes Th2- and Th1-mediated airway inflammation responses ( 13 ). (frontiersin.org)
  • Transient Receptor Potential Ankyrin 1 Enhances Ovalbumin-Induced Acute Allergic Inflammation in Murine Models. (bioportfolio.com)
  • Alternaria-induced allergic inflammation to Ovalbumin (OVA) challenge is suppressed by H. polygyrus Excretory-Secretory (HES) productsA. (nih.gov)
  • Our studies show the effectiveness and specificity of the hMSCs on decreasing chronic airway inflammation associated with the murine ovalbumin model of asthma. (physiology.org)
  • Paper III: Li-Zhi Suna, Thien Van Dob, Erik Florvaag, Nils P Aardald, Jørn Skavlanda and Kirsi Vaali, Toluene diisocyanate-induced bronchial hyper-reactivity and neutrophilic inflammation deviates from the eosinophil-dominant response to ovalbumin. (uib.no)
  • Huang, M. Morin attenuates ovalbumin-induced airway inflammation by modulating oxidative stress-responsive MAPK signaling. (eurekaselect.com)
  • Jiang, X. Downregulation of SUMF2 gene in ovalbumin-induced rat model of allergic inflammation. (eurekaselect.com)
  • Radiation-denatured and thermolabile proteins exhibited a low, essentially constant and doseunrelated serologic correspondence to native ovalbumin. (jimmunol.org)
  • Airway hyperresponsiveness to cigarette smoke in ovalbumin-sensitized guinea pigs. (escholarship.org)
  • This study was carried out to determine if the bronchoconstrictive effect of cigarette smoke (CS) is enhanced when airway hyperresponsiveness is induced by ovalbumin (Ova) sensitization, and if so, whether an increase in endogenously released tachykinins is involved. (escholarship.org)
  • Anti-MIF Ab significantly decreased the number of total cells, neutrophils and eosinophils in the bronchoalveolar lavage fluid of the ovalbumin-challenged rats, and also attenuated the ovalbumin-induced airway hyperresponsiveness to ovalbumin and methacholine. (elsevier.com)
  • Mouse anti-Ovalbumin IgE Std A, (0 ng/ml), 0.65ml 600-165-A3. (4adi.com)
  • Mouse anti-Ovalbumin IgE Std B, (15.6 ng/ml), 0.65 ml 600-165-B4. (4adi.com)
  • Mouse anti-Ovalbumin IgE Std C, (31.2 ng/ml), 0.650 ml 600-165-C5. (4adi.com)
  • Mouse anti-Ovalbumin IgE Std D, (62.5 ng/ml), 0.650 ml 600-165-D6. (4adi.com)
  • Mouse anti-Ovalbumin IgE Std E, (125 ng/ml), 0.650 ml 610-165-E7. (4adi.com)
  • Mouse anti-Ovalbumin IgE Std F, (250 ng/ml), 0.650 ml 600-165-F8. (4adi.com)
  • Characterization of the steroid-dependent regulatory element (SDRE) in the check ovalbumin gene (-892 to -796) suggests that it functions as a hormone response unit. (ebscohost.com)
  • Transfection experiments using linker scanning and point mutations support the contention that the binding of these three complexes is essential for induction of the ovalbumin gene by steroid hormones. (ebscohost.com)
  • Saturation kinetics showed that 2-fold less receptor was required for half-maximal binding to ovalbumin gene flanking sequences than to plasmid pBR322 sequences. (springer.com)
  • M. LeMeur, N. Glanville, J. L. Mandel, P. Gerlinger, R. Palmiter, P. Chambon, The ovalbumin gene family: Hormonal control of X and Y gene transcription and mRNA accumulation. (springer.com)
  • The expression of the ovalbumin gene in hen oviduct decreases during ageing. (mysciencework.com)
  • The ovalbumin gene. (scripps.edu)
  • Purified ovalbumin messenger RNA was employed to selectively enrich the concentration of the gene coding for ovalbumin from total chick DNA by molecular hybridization. (scripps.edu)
  • The coding strand of the ovalbumin gene was partially purified from sheared chick DNA by affinity column chromatography using ovalbumin mRNA immobilized on phosphocellulose. (scripps.edu)
  • Since ovalbumin mRNA has a complexity of 1890 nucleotides, the resulting DNA was more than twice the length of ovalbumin mRNA and thus should contain DNA sequences adjacent to the structural portion of the ovalbumin gene. (scripps.edu)
  • Pulmonary dendritic cell distribution and prevalence in guinea pig airways: effect of ovalbumin sensitization and challenge. (cdc.gov)
  • OBJECTIVE To study the humoral immune response to bovine serum albumin (BSA) and ovalbumin (OA) in children with newly diagnosed insulin-dependent diabetes mellitus (IDDM). (diabetesjournals.org)
  • Thermo Scientific™ Imject Maleimide-Activated Ovalbumin is sulfhydryl-reactive ovalbumin (OVA) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens. (fishersci.ca)
  • Thermogravimetric analysis showed that the maximum weight loss point of ovalbumin/curcumin complex was at 289 °C, which was lower than that of pure ovalbumin and curcumin, implying that the complex was more prone to pyrolyze. (deepdyve.com)
  • Furthermore, the improved antioxidant activity was evaluated by DPPH radical scavenging activity and reducing power assay compared with pure ovalbumin and curcumin. (deepdyve.com)
  • Amongst others egg powder as well as pure ovalbumin is often used as fining reagent for wine. (eurofins-technologies.com)
  • Actinomycin D and thioacetamide induced ovalbumin synthesis and increased serum progesterone concentrations in immature chicks. (sciencemag.org)
  • Ovalbumin binding in serum, 6 weeks after immunisation via Peyer's patches was higher than binding detected following oral or intra-ileal immunisation. (lincoln.ac.nz)
  • This kit measure total anti-ovalbumin IgE in mouse serum. (4adi.com)
  • However, anti-MIF Ab did not affect the level of serum ovalbumin-specific IgE, suggesting that anti-MIF Ab did not suppress immunisation itself. (elsevier.com)
  • The aim of this work was to evaluate the properties of poly-epsilon-caprolactone (PCL) microparticles as adjuvants in immunotherapy using ovalbumin (OVA) as an allergen model. (cun.es)
  • Dose-dependent thiol and immune responses to ovalbumin challenge in Brown Norway rats. (cdc.gov)
  • Maloy, K.J. , Donachie, A.M. , O'Hagan, D.T. and Mowat, A.M. (1994) Induction of mucosal and systemic immune responses by immunization with ovalbumin entrapped in poly(lactide-co-glycolide) microparticles. (gla.ac.uk)
  • Adjuvant effect of aqueous extract of Rhodiola imbricata rhizome on the immune responses to tetanus toxoid and ovalbumin in rats. (semanticscholar.org)
  • Although FL treatment induces up to a 10,000-fold increase in ovalbumin-specific IgG2a and a more modest increase in IgG1 titers, GM-CSF treatment favors a predominantly IgG1 response with little increase in IgG2a levels. (pnas.org)
  • The purpose of the present study was to determine the effects of orally administered bromelain in an ovalbumin (OVA)-induced murine model of acute allergic airway disease (AAD). (pubmedcentralcanada.ca)
  • This study aimed to evaluate the preventive activity of IMP in an ovalbumin (OVA)-induced murine model of asthma and to investigate its possible molecular mechanisms. (ovid.com)
  • Aim: To determine the efficacy of murine model of ovalbumin (OVA)-induced AHR inhibition by morin and decipher the molecular mechanism involved. (eurekaselect.com)
  • We have other isotype specific anti-ovalbumin kit available see related items). (4adi.com)
  • MBS730660 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ovalbumin specific Immunoglobulin E (OVA slgE) ELISA Kit target analytes in biological samples. (mybiosource.com)
  • Cross reactivity with ovalbumins from other avian species has not been tested. (novusbio.com)
  • In the current study, the phenotypic characteristics of lung interstitial macrophages were investigated in an ovalbumin (OVA)‑induced mouse model of asthma. (spandidos-publications.com)
  • An immunohistochemical study revealed positive immunostaining for MIF in bronchial epithelium, even in nonsensitised rats, and the MIF staining in bronchial epithelium was enhanced after the ovalbumin challenge. (elsevier.com)
  • We characterized the localization and prevalence of dendritic cells (DC) in guinea pig airways before and after s.c. sensitization and aerosol challenge with ovalbumin (OVA). (cdc.gov)
  • Four weeks after sensitization with ovalbumin (0.1 mg x k(-1)), the guinea-pigs were anaesthetized and mechanically ventilated. (ersjournals.com)
  • This study aims to evaluate the effect of KOB03 on ovalbumin (OVA)-induced allergic rhinitis (AR) in guinea pigs. (springer.com)
  • Our previous study demonstrated that a complex traditional Chinese medicine (CTCM) administered in nebulized form through the intratracheal route is effective against early-phase air-flow obstruction and can inhibit IL-5 production in ovalbumin (OVA)-sensitized guinea pigs. (biomedcentral.com)
  • BUF048 is a preparation of HRP conjugated ovalbumin, which may be used for the measurement of ovalbumin specific IgE in immunoassay systems, without species restriction. (bio-rad-antibodies.com)
  • Northern transfer of electrophoretically fractionated total RNA from oviducts of mature egg-laying and old non-egg-laying hens, and hybridization to nick-translated plasmid pOV230 (ovalbumin complementary DNA cloned into pMB9) revealed with age increasing concentrations of distinct high-molecular-weight ovalbumin pre-mRNA species as compared with that of functional 18S ovalbumin mRNA. (mysciencework.com)
  • Similar to ovalbumin and OVAY, OVAX belongs to the ovalbumin serine protease inhibitor family (ov-serpin). (sigmaaldrich.com)
  • Purified, carrier-grade ovalbumin has been modified with SMCC crosslinker to attach maleimide groups capable of forming covalent crosslinks with sulfhydryl (-SH) moieties on cysteine residues of peptides and other molecules. (fishersci.ca)
  • Ovalbumin is a member of serpin superfamily, but it does not have any proteinase inhibitor activity. (nii.ac.jp)
  • The functional signal for membrane translocation of ovalbumin becomes accessible when the nascent chain is 50 to 60 residues long. (abcam.com)
  • The SENSI Spec Ovalbumin ELISA represents a highly sensitive detection system for ovalbumin and is particularly capable of the quantification of ovalbumin residues in wine. (eurofins-technologies.com)
  • Thus for the detection of ovalbumin residues, sensitive assay systems are required. (eurofins-technologies.com)
  • Ovalbumin Standard A, 0 ng/ml, 0.65 ml 6052A3. (4adi.com)
  • Ovalbumin Standard B, 0.2 ng/ml, 0.65 ml 6052B4. (4adi.com)
  • Ovalbumin Standard C, 0.5 ng/ml, 0.65 ml 6052C5. (4adi.com)
  • Ovalbumin Standard D, 1.0 ng/ml, 0.65 ml 6052D6. (4adi.com)
  • Ovalbumin Standard E, 2.0 ng/ml, 0.65 ml 6052E7. (4adi.com)
  • Ovalbumin Standard F, 4.0 ng/ml, 0.65 ml 6052F8. (4adi.com)
  • Osmotic pressure measurements of ovalbumin and lysozyme mixtures. (biomedsearch.com)
  • Ovalbumin and lysozyme have been reported to undergo a mixed association in solutions of low ionic strength. (biomedsearch.com)
  • Osmotic pressure experiments on three blends of lysozyme and ovalbumin at 30 and 37 degrees C could be interpreted in two ways. (biomedsearch.com)
  • In addition to lysozyme, ovomucoid ovotransferrin and livetin, ovalbumin represents the main fraction of the egg white allergens. (eurofins-technologies.com)
  • G. S. McKnight, R. D. Palmiter, Transcriptional regulation of the ovalbumin and conalbumin genes by steroid hormones in chick oviduct. (springer.com)
  • In addition, ovalbumin binding of oviduct and ileal secretions in animals immunised via Peyer's patches was higher than the binding in animals immunised directly into the ileum. (lincoln.ac.nz)
  • Evidence for age-dependent impairment of ovalbumin heterogeneous nuclear RNA (HnRNA) processing in hen oviduct. (mysciencework.com)
  • Shows high affinity for Ovalbumin in its native form. (abcam.com)
  • Binding data from a variety of different restriction digests suggest receptor A interacts between −135 and −249 bp (upstream) from the start of ovalbumin transcription. (springer.com)