A cardioactive glycoside consisting of rhamnose and ouabagenin, obtained from the seeds of Strophanthus gratus and other plants of the Apocynaceae; used like DIGITALIS. It is commonly used in cell biological studies as an inhibitor of the NA(+)-K(+)-EXCHANGING ATPASE.
An enzyme that catalyzes the active transport system of sodium and potassium ions across the cell wall. Sodium and potassium ions are closely coupled with membrane ATPase which undergoes phosphorylation and dephosphorylation, thereby providing energy for transport of these ions against concentration gradients.
A member of the alkali group of metals. It has the atomic symbol Na, atomic number 11, and atomic weight 23.
An element in the alkali group of metals with an atomic symbol K, atomic number 19, and atomic weight 39.10. It is the chief cation in the intracellular fluid of muscle and other cells. Potassium ion is a strong electrolyte that plays a significant role in the regulation of fluid volume and maintenance of the WATER-ELECTROLYTE BALANCE.
Cyclopentanophenanthrenes with a 5- or 6-membered lactone ring attached at the 17-position and SUGARS attached at the 3-position. Plants they come from have long been used in congestive heart failure. They increase the force of cardiac contraction without significantly affecting other parameters, but are very toxic at larger doses. Their mechanism of action usually involves inhibition of the NA(+)-K(+)-EXCHANGING ATPASE and they are often used in cell biological studies for that purpose.
The movement of materials across cell membranes and epithelial layers against an electrochemical gradient, requiring the expenditure of metabolic energy.
A cardiotonic glycoside obtained mainly from Digitalis lanata; it consists of three sugars and the aglycone DIGOXIGENIN. Digoxin has positive inotropic and negative chronotropic activity. It is used to control ventricular rate in ATRIAL FIBRILLATION and in the management of congestive heart failure with atrial fibrillation. Its use in congestive heart failure and sinus rhythm is less certain. The margin between toxic and therapeutic doses is small. (From Martindale, The Extra Pharmacopoeia, 30th ed, p666)
An element that is an alkali metal. It has an atomic symbol Rb, atomic number 37, and atomic weight 85.47. It is used as a chemical reagent and in the manufacture of photoelectric cells.
A cardiac glycoside sometimes used in place of DIGOXIN. It has a longer half-life than digoxin; toxic effects, which are similar to those of digoxin, are longer lasting. (From Martindale, The Extra Pharmacopoeia, 30th ed, p665)
Unstable isotopes of rubidium that decay or disintegrate emitting radiation. Rb atoms with atomic weights 79-84, and 86-95 are radioactive rubidium isotopes.
Stable sodium atoms that have the same atomic number as the element sodium, but differ in atomic weight. Na-23 is a stable sodium isotope.
A number of different cardioactive glycosides obtained from Strophanthus species. OUABAIN is from S. gratus and CYMARINE from S. kombe. They are used like the digitalis glycosides.
Agents that have a strengthening effect on the heart or that can increase cardiac output. They may be CARDIAC GLYCOSIDES; SYMPATHOMIMETICS; or other drugs. They are used after MYOCARDIAL INFARCT; CARDIAC SURGICAL PROCEDURES; in SHOCK; or in congestive heart failure (HEART FAILURE).
Glycosides from plants of the genus DIGITALIS. Some of these are useful as cardiotonic and anti-arrhythmia agents. Included also are semi-synthetic derivatives of the naturally occurring glycosides. The term has sometimes been used more broadly to include all CARDIAC GLYCOSIDES, but here is restricted to those related to Digitalis.
A type of glycoside widely distributed in plants. Each consists of a sapogenin as the aglycone moiety, and a sugar. The sapogenin may be a steroid or a triterpene and the sugar may be glucose, galactose, a pentose, or a methylpentose.
Cyclopentanophenanthrenes with a 6-membered lactone ring attached at the 17-position and SUGARS attached at the 3-position. They are found in BUFONIDAE and often possess cardiotonic properties.
A compound that inhibits symport of sodium, potassium, and chloride primarily in the ascending limb of Henle, but also in the proximal and distal tubules. This pharmacological action results in excretion of these ions, increased urinary output, and reduction in extracellular fluid. This compound has been classified as a loop or high ceiling diuretic.
An element in the alkali metals family. It has the atomic symbol Li, atomic number 3, and atomic weight [6.938; 6.997]. Salts of lithium are used in treating BIPOLAR DISORDER.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
The aglycone constituents of CARDIAC GLYCOSIDES. The ring structure is basically a cyclopentanoperhydrophenanthrene nucleus attached to a lactone ring at the C-17 position.
A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.
Stable potassium atoms that have the same atomic number as the element potassium, but differ in atomic weight. K-41 is a stable potassium isotope.
The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).
A pyrazine compound inhibiting SODIUM reabsorption through SODIUM CHANNELS in renal EPITHELIAL CELLS. This inhibition creates a negative potential in the luminal membranes of principal cells, located in the distal convoluted tubule and collecting duct. Negative potential reduces secretion of potassium and hydrogen ions. Amiloride is used in conjunction with DIURETICS to spare POTASSIUM loss. (From Gilman et al., Goodman and Gilman's The Pharmacological Basis of Therapeutics, 9th ed, p705)
Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.
An alpha-2 selective adrenergic agonist used as an antihypertensive agent.
A sulfamyl diuretic.
A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.
A benzoic-sulfonamide-furan. It is a diuretic with fast onset and short duration that is used for EDEMA and chronic RENAL INSUFFICIENCY.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
A subclass of GLYCOSPHINGOLIPIDS containing one or more sugars within their head group connected directly to a ceramide moiety. They consist of monoglycosyl-, and oligoglycosylsphingoids and monoglycosyl- and oligoglycosylceramides.
An enzyme that hydrolyzes thiamine pyrophosphate to thiamine monophosphate plus inorganic phosphate. EC 3.6.1.-.
The thin layer of GRAY MATTER on the surface of the CEREBRAL HEMISPHERES that develops from the TELENCEPHALON and folds into gyri and sulchi. It reaches its highest development in humans and is responsible for intellectual faculties and higher mental functions.
The domestic cat, Felis catus, of the carnivore family FELIDAE, comprising over 30 different breeds. The domestic cat is descended primarily from the wild cat of Africa and extreme southwestern Asia. Though probably present in towns in Palestine as long ago as 7000 years, actual domestication occurred in Egypt about 4000 years ago. (From Walker's Mammals of the World, 6th ed, p801)
Injections made into a vein for therapeutic or experimental purposes.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
3 beta,5,14-Trihydroxy-19-oxo-5 beta-card-20(22)-enolide. The aglycone cardioactive agent isolated from Strophanthus Kombe, S. gratus and other species; it is a very toxic material formerly used as digitalis. Synonyms: Apocymarin; Corchorin; Cynotoxin; Corchorgenin.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
The residual portion of BLOOD that is left after removal of BLOOD CELLS by CENTRIFUGATION without prior BLOOD COAGULATION.
The active sympathomimetic hormone from the ADRENAL MEDULLA. It stimulates both the alpha- and beta- adrenergic systems, causes systemic VASOCONSTRICTION and gastrointestinal relaxation, stimulates the HEART, and dilates BRONCHI and cerebral vessels. It is used in ASTHMA and CARDIAC FAILURE and to delay absorption of local ANESTHETICS.
A PEPTIDE that is secreted by the BRAIN and the HEART ATRIA, stored mainly in cardiac ventricular MYOCARDIUM. It can cause NATRIURESIS; DIURESIS; VASODILATION; and inhibits secretion of RENIN and ALDOSTERONE. It improves heart function. It contains 32 AMINO ACIDS.
PRESSURE of the BLOOD on the ARTERIES and other BLOOD VESSELS.
An octapeptide that is a potent but labile vasoconstrictor. It is produced from angiotensin I after the removal of two amino acids at the C-terminal by ANGIOTENSIN CONVERTING ENZYME. The amino acid in position 5 varies in different species. To block VASOCONSTRICTION and HYPERTENSION effect of angiotensin II, patients are often treated with ACE INHIBITORS or with ANGIOTENSIN II TYPE 1 RECEPTOR BLOCKERS.
Precursor of epinephrine that is secreted by the adrenal medulla and is a widespread central and autonomic neurotransmitter. Norepinephrine is the principal transmitter of most postganglionic sympathetic fibers and of the diffuse projection system in the brain arising from the locus ceruleus. It is also found in plants and is used pharmacologically as a sympathomimetic.
The number of times the HEART VENTRICLES contract per unit of time, usually per minute.
Adverse functional, metabolic, or structural changes in ischemic tissues resulting from the restoration of blood flow to the tissue (REPERFUSION), including swelling; HEMORRHAGE; NECROSIS; and damage from FREE RADICALS. The most common instance is MYOCARDIAL REPERFUSION INJURY.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
Pathological processes of the KIDNEY or its component tissues.
Abrupt reduction in kidney function. Acute kidney injury encompasses the entire spectrum of the syndrome including acute kidney failure; ACUTE KIDNEY TUBULAR NECROSIS; and other less severe conditions.
Long convoluted tubules in the nephrons. They collect filtrate from blood passing through the KIDNEY GLOMERULUS and process this filtrate into URINE. Each renal tubule consists of a BOWMAN CAPSULE; PROXIMAL KIDNEY TUBULE; LOOP OF HENLE; DISTAL KIDNEY TUBULE; and KIDNEY COLLECTING DUCT leading to the central cavity of the kidney (KIDNEY PELVIS) that connects to the URETER.
Excision of kidney.

PST 2238: A new antihypertensive compound that modulates Na,K-ATPase in genetic hypertension. (1/3013)

A genetic alteration in the adducin genes is associated with hypertension and up-regulation of the expression of renal Na, K-ATPase in Milan-hypertensive (MHS) rats, in which increased ouabain-like factor (OLF) levels are also observed. PST 2238, a new antihypertensive compound that antagonizes the pressor effect of ouabain in vivo and normalizes ouabain-dependent up-regulation of the renal Na-K pump, was evaluated for its ability to lower blood pressure and regulate renal Na,K-ATPase activity in MHS genetic hypertension. In this study, we show that PST 2238, given orally at very low doses (1 and 10 microg/kg for 5-6 weeks), reduced the development of hypertension in MHS rats and normalized the increased renal Na,K-ATPase activity and mRNA levels, whereas it did not affect either blood pressure or Na,K-ATPase in Milan-normotensive (MNS) rats. In addition, a similar antihypertensive effect was observed in adult MHS rats after a short-term treatment. In cultured rat renal cells with increased Na-K pump activity at Vmax due to overexpression of the hypertensive variant of adducin, 5 days of incubation with PST 2238 (10(-10-)-10(-9) M) lowered the pump rate to the level of normal wild-type cells, which in turn were not affected by the drug. In conclusion, PST 2238 is a very potent compound that in MHS rats reduces blood pressure and normalizes Na-K pump alterations caused by a genetic alteration of the cytoskeletal adducin. Because adducin gene mutations have been associated with human essential hypertension, it is suggested that PST 2238 may display greater antihypertensive activity in those patients carrying such a genetic alteration.  (+info)

Fluid secretion by the malpighian tubules of the tsetse fly Glossina morsitans: the effects of ouabain, ethacrynic acid and amiloride. (2/3013)

The effects of three inhibitors of sodium transport on the secretion of fluid by the Malpighian tubules of Glossina morsitans have been observed. The cardiac glycoside, ouabain, affects neither the rate of secretion nor the sodium concentration of the fluid secreted when isolated tubules are bathed by solutions containing a range of sodium and potassium concentrations. Secretion is inhibited, however, by ethacrynic acid and amiloride. The results confirm that fluid secretion by the Malpighian tubules of this insect is dependent on the active transport of sodium ions and show that Na+/k+ exchange pumps are not involved in this process.  (+info)

Alterations of heart function and Na+-K+-ATPase activity by etomoxir in diabetic rats. (3/3013)

To examine the role of changes in myocardial metabolism in cardiac dysfunction in diabetes mellitus, rats were injected with streptozotocin (65 mg/kg body wt) to induce diabetes and were treated 2 wk later with the carnitine palmitoyltransferase inhibitor (carnitine palmitoyltransferase I) etomoxir (8 mg/kg body wt) for 4 wk. Untreated diabetic rats exhibited a reduction in heart rate, left ventricular systolic pressure, and positive and negative rate of pressure development and an increase in end-diastolic pressure. The sarcolemmal Na+-K+-ATPase activity was depressed and was associated with a decrease in maximal density of binding sites (Bmax) value for high-affinity sites for [3H]ouabain, whereas Bmax for low-affinity sites was unaffected. Treatment of diabetic animals with etomoxir partially reversed the depressed cardiac function with the exception of heart rate. The high serum triglyceride and free fatty acid levels were reduced, whereas the levels of glucose, insulin, and 3,3',-5-triiodo-L-thyronine were not affected by etomoxir in diabetic animals. The activity of Na+-K+-ATPase expressed per gram heart weight, but not per milligram sarcolemmal protein, was increased by etomoxir in diabetic animals. Furthermore, Bmax (per g heart wt) for both low-affinity and high-affinity binding sites in control and diabetic animals was increased by etomoxir treatment. Etomoxir treatment also increased the depressed left ventricular weight of diabetic rats and appeared to increase the density of the sarcolemma and transverse tubular system to normalize Na+-K+-ATPase activity. Therefore, a shift in myocardial substrate utilization may represent an important signal for improving the depressed cardiac function and Na+-K+-ATPase activity in diabetic rat hearts with impaired glucose utilization.  (+info)

A chimeric gastric H+,K+-ATPase inhibitable with both ouabain and SCH 28080. (4/3013)

2-Methyl-8-(phenylmethoxy)imidazo(1,2-a)pyridine-3acetonitrile+ ++ (SCH 28080) is a K+ site inhibitor specific for gastric H+,K+-ATPase and seems to be a counterpart of ouabain for Na+,K+-ATPase from the viewpoint of reaction pattern (i.e. reversible binding, K+ antagonism, and binding on the extracellular side). In this study, we constructed several chimeric molecules between H+,K+-ATPase and Na+,K+-ATPase alpha-subunits by using rabbit H+,K+-ATPase as a parental molecule. We found that the entire extracellular loop 1 segment between the first and second transmembrane segments (M1 and M2) and the luminal half of the M1 transmembrane segment of H+, K+-ATPase alpha-subunit were exchangeable with those of Na+, K+-ATPase, respectively, preserving H+,K+-ATPase activity, and that these segments are not essential for SCH 28080 binding. We found that several amino acid residues, including Glu-822, Thr-825, and Pro-829 in the M6 segment of H+,K+-ATPase alpha-subunit are involved in determining the affinity for this inhibitor. Furthermore, we found that a chimeric H+,K+-ATPase acquired ouabain sensitivity and maintained SCH 28080 sensitivity when the loop 1 segment and Cys-815 in the loop 3 segment of the H+,K+-ATPase alpha-subunit were simultaneously replaced by the corresponding segment and amino acid residue (Thr) of Na+,K+-ATPase, respectively, indicating that the binding sites of ouabain and SCH 28080 are separate. In this H+, K+-ATPase chimera, 12 amino acid residues in M1, M4, and loop 1-4 that have been suggested to be involved in ouabain binding of Na+, K+-ATPase alpha-subunit are present; however, the low ouabain sensitivity indicates the possibility that the sensitivity may be increased by additional amino acid substitutions, which shift the overall structural integrity of this chimeric H+,K+-ATPase toward that of Na+,K+-ATPase.  (+info)

Multiple mechanisms confer drug resistance to mitoxantrone in the human 8226 myeloma cell line. (5/3013)

Selection for in vitro drug resistance can result in a complex phenotype with more than one mechanism of resistance emerging concurrently or sequentially. We examined emerging mechanisms of drug resistance during selection with mitoxantrone in the human myeloma cell line 8226. A novel transport mechanism appeared early in the selection process that was associated with a 10-fold resistance to mitoxantrone in the 8226/MR4 cell line. The reduction in intracellular drug concentration was ATP-dependent and ouabain-insensitive. The 8226/MR4 cell line was 34-fold cross-resistant to the fluorescent aza-anthrapyrazole BBR 3390. The resistance to BBR 3390 coincided with a 50% reduction in intracellular drug concentration. Confocal microscopy using BBR 3390 revealed a 64% decrease in the nuclear:cytoplasmic ratio in the drug-resistant cell line. The reduction in intracellular drug concentration of both mitoxantrone and BBR 3390 was reversed by a novel chemosensitizing agent, fumitremorgin C. In contrast, fumitremorgin C had no effect on resistance to mitoxantrone or BBR 3390 in the P-glycoprotein-positive 8226/DOX6 cell line. Increasing the degree of resistance to mitoxantrone in the 8226 cell line from 10 to 37 times (8226/MR20) did not further reduce the intracellular drug concentration. However, the 8226/MR20 cell line exhibited 88 and 70% reductions in topoisomerase II beta and alpha expression, respectively, compared with the parental drug sensitive cell line. This decrease in topoisomerase expression and activity was not observed in the low-level drug-resistant, 8226/MR4 cell line. These data demonstrate that low-level mitoxantrone resistance is due to the presence of a novel, energy-dependent drug efflux pump similar to P-glycoprotein and multidrug resistance-associated protein. Reversal of resistance by blocking drug efflux with fumitremorgin C should allow for functional analysis of this novel transporter in cancer cell lines or clinical tumor samples. Increased resistance to mitoxantrone may result from reduced intracellular drug accumulation, altered nuclear/cytoplasmic drug distribution, and alterations in topoisomerase II activity.  (+info)

Electrophysiologic effect of enalapril on guinea pig papillary muscles in vitro. (6/3013)

AIM: To study the direct effect of enalapril on cellular electrophysiology of myocardium. METHODS: Conventional microelectrodes technique was used to record the action potentials (AP) of guinea pig papillary muscles. RESULTS: Enalapril caused an increase of the AP amplitude (APA) and the resting potential (RP) in a concentration-dependent manner without any significant change of AP duration, Vmax and overshoot of AP. Superfusion of ouabain 0.5 mumol.L-1 reduced APA and RP, induced stable delayed after-depolarizations (DAD) at different basic cycle lengths (BCL) in a frequency-dependent manner. At BCL 200 ms, the amplitude of DAD was large enough to induce nonsustained triggered activity (TA). In additional presence of enalapril 10 mumol.L-1, the DAD amplitude at 500, 400, 300, and 200 ms were decreased from 5.3 +/- 2.3, 5.9 +/- 2.8, 7.4 +/- 2.1, and 8.9 +/- 1.3 to 2.6 +/- 0.7, 3.1 +/- 1.0, 3.7 +/- 1.5, and 5.3 +/- 1.1 (mV) respectively, all P < 0.01. The compensation intervals were increased in a similar frequency-dependent manner. The number of TA induced at BCL 200 ms was decreased from 3.6 +/- 0.7 to 0.8 +/- 0.2 (P < 0.05). CONCLUSION: Enalapril directly inhibits DAD and TA induced by ouabain through increasing RP and APA, which may contribute to its anti-arrhythmic effect.  (+info)

Role of K+ channels in A2A adenosine receptor-mediated dilation of the pressurized renal arcuate artery. (7/3013)

1. Adenosine A2A receptor-mediated renal vasodilation was investigated by measuring the lumenal diameter of pressurized renal arcuate arteries isolated from the rabbit. 2. The selective A2A receptor agonist CGS21680 dilated the arteries with an EC50 of 130 nM. The CGS21680-induced vasodilation was, on average, 34% less in endothelium-denuded arteries. 3. The maximum response and the EC50 for CGS21680-induced vasodilation in endothelium-intact arteries were not significantly affected by incubation with the K+ channel blockers apamin (100 nM), iberiotoxin (100 nM), 3,4-diaminopyridine (1 mM), glibenclamide (1 microM) or Ba2+ (10 microM). However, a cocktail mixture of these blockers did significantly inhibit the maximum response by almost 40%, and 1 mM Ba2+ alone or 1 mM Ba2+ in addition to the cocktail inhibited the maximum CGS21680-response by 58% and about 75% respectively. 4. CGS21680-induced vasodilation was strongly inhibited when the extracellular K+ level was raised to 20 mM even though the dilator response to 1 microM levcromakalim, a K(ATP) channel opener drug, was unaffected. 5. CGS21680-induced vasodilation was inhibited by 10 microM ouabain, an inhibitor of Na+/K(+)-ATPase, but ouabain had a similar inhibitory effect on vasodilation induced by 30 nM nicardipine (a dihydropyridine Ca2+ antagonist) or 1 microM levcromakalim. 6. The data suggest that K+ channel activation does play a role in A(2A) receptor-mediated renal vasodilation. The inhibitory effect of raised extracellular K+ levels on the A(2A) response may be due to K(+)-induced stimulation of Na+/K(+)-ATPase.  (+info)

Excretion of taurocholate from isolated hepatocytes. (8/3013)

Efflux of taurocholate from isolated rat hepatocytes was studied to characterize the mechanism of bile acid secretion. Cells were incubated with taurocholate for 15 min. The amount of the intracellularly accumulated bile acid was directly related to the concentration in the medium. Transfer of the loaded cells from the incubation medium to a medium without taurocholate led to taurocholate efflux. Efflux was saturable, its activation energy amounted to 12 kcal/mol (50 kJ). It was strongly inhibited by the metabolic inhibitor antimycin A and to a lesser extend by the uncoupler carbonylcyanide-m-chlorophenylhydrazone. Dinitrofluorobenzene and mersalyl, reagents which react with amino acids, inhibited efflux by about 30% when applied at concentrations of 50 muM. Ouabain increased the rate of efflux. The observations indicate that secretory functions are maintained in isolated liver cells.  (+info)

The effects of ouabain or ouabain and furosemide on renal function and renin secretion were studied in conscious isovolemic sheep. The sheep received a continuous renal arterial infusion of papaverine, 7 mg/min, throughout the experiment. Ouabain alone (7 X 10(-7) M in the renal plasma) produced significant decreases in glomerular filtration rate (GFR) and renal plasma flow (RPF) but not in renal perfusion pressure. Plasma [K+] rose after ouabain administration. Fractional (FENa) and absolute (UNaV) Na+ excretion were 2.9 +/- 1.0% (mean +/- SE) and 78 +/- 54 muEq/min, respectively, during the papaverine infusion and rose to 19 +/- 5.1% (P less than 0.05) and 528 +/- 116 muEq/min (P less than 0.01) after ouabain administration. Despite the large changes in Na+ reabsorption, renin secretion was not stimulated. During the control period, renin secretion was 281 +/- 131 ng/min and the average renin secretion after ouabain administration was 310 +/- 78 ng/min (not significant). A smaller dose of ...
Changes in the sodium/potassium-adenosine triphosphatase (Na+/K+ pump) of human leukocytes may reflect changes in the Na+/K+ pump of vascular smooth muscle cells in cryptogenic (essential) hypertension. Lymphocytes from healthy donors have significantly fewer Na+/K+ pumps than normal when incubated for 72 h with serum from patients with crytogenic hypertension. This effect might be due to Na+/K+ pump inhibition by a cardiac glycoside-like substance in the serum. We assessed the sensitivity of two techniques for measuring inhibition of cellular (Na+/K+ pumps by ouabain at low concentrations in vitro. We incubated lymphocytes from healthy subjects for 1 h and 72 h with unlabelled ouabain at different concentrations to determine the lowest concentration which inhibits specific 3H-ouabain binding (the number of Na+/K+ pumps) and ouabain sensitive 86rubidium influx (pump activity). Incubation of cells with ouabain for 1 h did not significantly affect Na+/K+ pump activity. The lowest ouabain ...
1. The influence of single-dose intravenous ouabain on haemodynamics, renal function and vasoactive hormones was investigated in healthy subjects.. 2. After ouabain injection there was a significant reduction in minimum and average hourly heart rates. Diastolic blood pressure fell immediately after ouabain, without a significant change in systolic blood pressure.. 3. There was no change in renal blood flow, glomerular filtration rate, hourly urine volume or sodium and potassium excretion.. 4. Ouabain injection was associated with a fall in plasma angiotensin II levels and a rise in plasma adrenaline levels, but plasma noradrenaline levels were unchanged. Both plasma atrial and brain natriuretic peptide levels were increased after ouabain administration.. 5. The present study demonstrates that ouabain has significant although minor, acute effects on plasma levels of vasoactive hormones. It fails, however, to confirm that ouabain is an acute pressor or natriuretic substance. If it has an important ...
TY - JOUR. T1 - Quantification of Rat Cerebral Cortex Na+,K+‐ATPase. T2 - Effect of Age and Potassium Depletion. AU - Schmidt, Thomas Andersen. AU - Larsen, Jim Stenfatt. AU - Kjeldsen, Keld. PY - 1992/12. Y1 - 1992/12. N2 - Abstract: Na+,K+‐ATPase concentration in rat cerebral cortex was studied by vanadate‐facilitated [3H]ouabain binding to intact samples and by K+‐dependent 3‐O‐methylfluorescein phosphatase activity determinations in crude homogenates. Methodological errors of both methods were evaluated. [3H]Ouabain binding to cerebral cortex obtained from 12‐week‐old rats measured incubating samples in buffer containing [3H]ouabain, and ouabain at a final concentration of 1 × 10-6 mol/L gave a value of 11,351 ± 177 (n = 5) pmol/g wet weight (mean ± SEM) without any significant variation between the lobes. Evaluation of affinity for ouabain was in agreement with a heterogeneous population of [3H]ouabain binding sites. K+‐dependent 3‐O‐methylfluorescein phosphatase ...
Warm renal ischemia performed during partial nephrectomy has been found to be associated with kidney disease. Since endogenous ouabain (EO) is a neuro-endocrine hormone involved in renal damage, we evaluated the role of EO in renal ischemia-reperfusion injury (IRI). We measured plasma and renal EO variations and markers of glomerular and tubular damage (nephrin, KIM-1, Kidney-Injury-Molecule-1, α1 Na-K ATPase) and the protective effect of the ouabain inhibitor, rostafuroxin. We studied five groups of rats: (1) normal; (2) infused for eight weeks with ouabain (30 µg/kg/day, OHR) or (3) saline; (4) ouabain; or (5) saline-infused rats orally treated with 100 µg/kg/day rostafuroxin for four weeks. In group 1, 2-3 h after IRI, EO increased in ischemic kidneys while decreased in plasma. Nephrin progressively decreased and KIM-1 mRNA increased starting from 24 h. Ouabain infusion (group 2) increased blood pressure (from 111.7 to 153.4 mmHg) and ouabain levels in plasma and kidneys. In OHR ischemic kidneys
Ouabain, that has been isolated from bovine adrenals and hypothalamus, is a new cardiotonic steroid hormone, which is either synthesized in the adrenals or stored there after it has absorbed from the diet. Little is known ,i,in vivo,/i, which events may lead to the release of ouabain into blood. Moreover, a binding protein for cardiotonic steroids exists in blood, which binds cardiac glycosides with high affinity. It may affect the action of endogenous ouabain on heart and circulation, but the physiological function of this protein is unclear. To realize, which physiological stimuli ,i,in vivo,/i, may affect blood concentrations of endogenous ouabain and which function the cardiotonic binding protein may have in modulating ouabain effects, the effect of physical exercise on endogenous ouabain was studied and the tissue distribution of its binding protein was investigated. We found that endogenous ouabain changes rapidly in blood upon physical exercise and behaves like expected for a hormone of ...
An enzyme-linked immunosorbent assay is described for the measurement of ouabain in human plasma. This assay is specific for ouabain, strophanthidin, and ouabagenin, with other steroids, including digoxin and vasopressor hormones, exhibiting negligible cross-reactivity. Assay sensitivity was 0.06 nmol/L if 1 mL plasma was extracted and less than 0.005 nmol/L when 20 mL plasma was analyzed. Extracted plasma samples showed ouabainlike immunoreactivity that diluted in parallel with the ouabain standard curve. Repeated extraction and assay of single plasma samples, however, did not produce consistent results in the assay. Increased specificity was obtained by high-performance liquid chromatography of sample extracts before assay. When high-performance liquid chromatographic profiles of plasma spiked with ouabain standard or following bolus intravenous injections of ouabain into normal human volunteers were compared with profiles of unspiked plasma, there was no support for the immunoreactive ...
The response of the left ventricle to a level of supine bicycle exercise that induced angina, and the effects of ouabain (0.01 mg/kg) on this response were evaluated in 14 patients with coronary arterial disease. Before administration of ouabain, exercise was associated with depressed pumping performance of the left ventricle in four patients: stroke work fell with exercise while left ventricular end-diastolic pressure (LVEDP) increased. In five patients left ventricular (LV) performance was not frankly depressed, but nevertheless was abnormal: stroke work increased, but was accompanied by an inordinately large rise in LVEDP. In the remaining five patients LV function was essentially normal. Ouabain improved LV performance at rest in only a minority of patients, but during the stress of exercise, LV hemodynamics were improved by ouabain in 12 of the 14 patients. Five patients, however, continued to show either depressed or abnormal function with exercise after they were given ouabain. As judged ...
By using random mutagenesis (for references, see Croyle et al., 1997) or chimeras between Na,K- and H,K-ATPase (Qiu et al., 2003), nearly 20 amino acids have been identified that influence the sensitivity of Na,K-ATPase to ouabain. These amino acids are located in several transmembrane segments and extracellular loops of the Na,K-ATPase α-subunit and are hypothesized to comprise the physical binding site or to affect indirectly ouabain binding by conformational changes. In this study, we have investigated the structural determinants that are responsible for the α isoform-specific differences in the ouabain binding kinetics and could identify new amino acids that are involved in ouabain binding and play a role in the access and release of ouabain to and from its binding site.. Results from previous kinetic studies of ouabain binding, which were performed mainly on Na,K-ATPase α1 isozymes, have suggested that the rate of association of ouabain to enzymes from different sources, both sensitive ...
1. The cardiac glycoside ouabain, in concentrations ranging from 2 to 3 x 10-7, increases the oxygen uptake of slices of guinea pig heart muscle in the presence of glucose or lactate; at higher concentrations this increase (maximally 50 per cent) is followed by a depression (maximally 80 per cent). Among a variety of other guinea pig tissues studied, brain cortex alone responds in a similar manner, but is only about ⅕ as sensitive as heart muscle.. 2. The oxidation of Succinate in cardiac slices is not inhibited at all by ouabain; in brain cortex slices it is inhibited only to a moderate extent. In the presence of pyruvate, the oxygen uptake of cardiac slices, which is high initially, is depressed by ouabain without preceding stimulation. The effect of the drug on the oxygen uptake of brain cortex slices is the same in pyruvate as in glucose or lactate medium.. 3. The increase in the respiration of cardiac slices produced by ouabain is dependent upon the presence and concentration of ...
Ouabain, a specific inhibitor of active cation transport across cell membrane, was applied topically to brain surface of cats. The cerebral cortex to which 10−3M ouabain was applied showed a spongy...
Langham, Maurice E., and Kenneth E. Eakin. THE INFLUENCE OF THE CARDIAC GLYCOSIDE OUABAIN ON THE INTRAOCULAR PRESSURE AND DYNAMICS OF THE RABBIT AND THE CAT. Journal of Pharmacology and Experimental Therapeutics 144.3 (1964): 421-428. Web. 12 Dec. 2017. ...
Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Magnesium atom in PDB 3b8e: Crystal Structure of the Sodium-Potassium Pump
TY - JOUR. T1 - Calcium and contractile responses to ouabain in aortae from spontaneously hypertensive rats (SHR). AU - Lamb, F. S.. AU - Moreland, R. S.. AU - Webb, R. C.. PY - 1984. Y1 - 1984. UR - http://www.scopus.com/inward/record.url?scp=17944387233&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=17944387233&partnerID=8YFLogxK. M3 - Article. AN - SCOPUS:17944387233. VL - 43. SP - no. 834. JO - Federation Proceedings. JF - Federation Proceedings. SN - 0014-9446. IS - 3. ER - ...
In the brain at least 40% of the energy produced by mitochondrial respiration is required by the Na+/K+-ATPase to maintain ion gradients across the cell membranes. Energy levels in the brain can be compromised by a lack of glucose and oxygen or by defects in the respiratory chain such as occurring in stroke and Parkinsons disease, respectively. Na+/K+-ATPase function is inhibited during energy failure. This may lead to a prolonged depolarization of the neuron, excessive release, and reversal of the uptake of excitatory amino acids, i.e., the induction of excitotoxicity (Dirnagl et al., 1999; Doble, 1999; Nicotera et al., 1999). Ouabain inhibits Na+/K+-ATPases and is a very potent neurotoxin that leads to pancellular necrosis and infarction (Lees, 1991). It is used to study the involvement of Na+/K+-ATPase in CNS pathology (Lees et al., 1990; Lees, 1991; Lees and Leong, 1994,1995; Stelmashook et al., 1999). Ouabain rapidly perturbs ion homeostasis and induces cell swelling and ...
The function of ouabain to act as a signal generator of the sodium pump opens up new therapeutic options and illustrates the therapeutic potential of ouabain
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1. We have measured specific [3H]ouabain binding and ouabain sensitive 86rubidium influx in intact human lymphocytes incubated for up to 7 days in media containing different concentrations of fetal calf serum and human serum.. 2. Incubation for periods of up to 7 days with fetal calf serum and human serum produced increases in both specific [3H]ouabain binding and ouabain sensitive 86rubidium influx that were dependent on concentration and time.. 3. Neither specific [3H]ouabain binding nor ouabain sensitive 86rubidium influx was altered when dialysed serum was used, suggesting that both fetal calf serum and human serum contain a dialysable factor or factors which stimulate specific [3H]ouabain binding and ouabain sensitive 86rubidium influx in intact human lymphocytes.. 4. To further elucidate the mechanisms underlying these changes we also measured the activity of two other enzymes of the lymphocyte plasma membrane, 5′-nucleotidase and γ-glutamyltransferase, the uptake of [3H]thymidine by ...
TY - JOUR. T1 - Suppression and potentiation of mouse hematopoietic progenitor cell proliferation by ouabain. AU - Spivak, J. L.. AU - Misiti, J.. AU - Stuart, R.. AU - Sharkis, S. J.. AU - Sensenbrenner, L. L.. PY - 1980. Y1 - 1980. N2 - Clonal assays for CFU-S, CFU-C, BFU-E, and CFU-E were employed to evaluate the effect of ouabain on the proliferation of mouse hematopoietic progenitor cells. Preincubation of bone marrow cells with ouabain at concentrations of 10-6-10-12 M suppressed the proliferation of CFU-S as measured by the spleen colony assay. At 10-9 M ouabain, spleen colony formation was inhibited by more than 95%. When included in soft agar cultures of bone marrow cells, ouabain suppressed the proliferation of CFU-C in a complex fashion. At 10-4 M ouabain, colony formation was suppressed by 70%, while at 10-6 M ouabain, CFU-C proliferation was normal. At 10-9 M ouabain, however, the number of colonies formed was only 70% of normal, and complete recovery was not obtained at 10-12 M ...
The effect of cardiac glycosides to increase cardiac inotropy by altering Ca2+ cycling is well known but still poorly understood. The studies described in this report focus on defining the effects of ouabain signaling on sarcoplasmic reticulum Ca2+-ATPase function. Rat cardiac myocytes treated with 50 μM ouabain demonstrated substantial increases in systolic and diastolic Ca2+ concentrations. The recovery time constant for the Ca2+ transient, τ, was significantly prolonged by ouabain. Exposure to 10 μM H2O2, which causes an increase in intracellular reactive oxygen species similar to that of 50 μM ouabain, caused a similar increase in τ. Concurrent exposure to 10 mM N-acetylcysteine or an aqueous extract from green tea (50 mg/ml) both prevented the increases in τ as well as the changes in systolic or diastolic Ca2+ concentrations. We also observed that 50 μM ouabain induced increases in developed pressure in addition to diastolic dysfunction in the isolated perfused rat heart. Coadministration of
Digitalis glycosides are major tools for the identification of the Na/K ATPase as well as the Na/K fluxes mediated by this transport system. Since its discovery 50 yr ago (Schatzmann, 1953), the inhibitory effect of these compounds on the Na/K pump has been confirmed repeatedly. There has been evidence to contrary effects, although. Thus, Hougen et al. (1981) reported a stimulatory effect of nanomolar concentrations of ouabain on 86Rb uptake in guinea pig atria. This effect could be suppressed by propranolol, or by prior depletion of endogenous pools of norepinephrine in the tissue, and it was regarded as an indirect effect elicited by norepinephrine released by an action of ouabain on nerve endings in the heart.. Several others have reported stimulating effects of low concentrations of cardiac glycosides on active Na/K transport in multicellular cardiac preparations, and also in the squid axon, it was noted that the inhibitory effect of ouabain on 22Na efflux was preceded by an up to 20% ...
TY - JOUR. T1 - Impact of neonatal treatment with cardioactive glycosides (digoxin, ouabain) on receptor binding capacity, blood level and cardiac function in the adult rat. Extension of the imprinting theory. AU - Csaba, G.. AU - Inczefi-Gonda, Ágnes. AU - Dobozy, O.. AU - Varró, A.. AU - Rablóczky, G.. PY - 1983. Y1 - 1983. N2 - 1. 1. A single neonatal treatment with a cardioactive glycoside (ouabain, digoxin) altered the response of the adult rat to digitaloid treatment. 2. 2. As demonstrated by RIA, re-exposure to digoxin at 2 months of age was followed within 30 min by a more than twofold increase in serum digoxin over the not pretreated control and, although a steady concentration decrease followed, the experimental rats still had a higher serum digoxin level than the controls at 4 h. 3. 3. In the not presensitized control group the serum digoxin peak appeared at 60 min at a considerably lower level than the 30-min maximum of the experimental rats. 4. 4. Neonatal pretreatment with ...
Urinary concentration of OLS was measured by a radioimmunoassay as described previously.22 Briefly, ouabain was conjugated to ovalbumin and keyhole limpet haemocyanin (KLH) and antisera to ouabain was raised in goats by injecting ouabain-ovalbumin and then boosting with ouabain-KLH conjugate. The antisera showed minimal cross-reactivity against ouabagenin (4%) and other related steroids. Tritiated ouabain ([21-22-3H]-ouabain, specific activity approximately 47 μCi/mmol), was diluted to 0.3 μCi/mL with assay buffer (phosphate-buffered saline, 0.1 mol/L, pH 7.4, containing 0.1% bovine serum albumin). Aliquots of 100 μL each of tracer solution, antiserum (final dilution 1:90 000), and urine or standard ouabain were added to polystyrene tubes. After mixing, the reaction mixture was incubated for 1 hour at 37°C or overnight at 4°C. The bound and free ouabains were then separated by adding 250 μL of cold 2.5% dextran-coated charcoal. The samples were mixed, allowed to stand for 10 to 15 minutes ...
Ouabain-like inhibitors are elevated in approximately 50% of patients with essential hypertension and patients with pregnancy-induced hypertension. These materials inhibit the alpha-2 isoform of the Na/K-ATPase in vascular myocytes and increase intracellular sodium levels. The latter enhances calcium entry mediated by the Na/Ca Exchanger (NCX) and results in increased blood pressure. Indeed, prolonged administration of ouabain induces hypertension in Sprague Dawley rats. Paradoxically, normal pregnancy is also associated with an increase in ouabain-like inhibitors, yet blood pressure decreases. My research is focused on the mechanism by which normal pregnant rats become resistant to ouabain-induced hypertension, and the role of NCX and pregnancy hormones in mediating this effect.. ...
Ubiquitously expressed sodium pumps are best known for maintaining the ionic gradients and resting membrane potential required for generating action potentials. However, activity- and state-dependent changes in pump activity can also influence neuronal firing and regulate rhythmic network output. Here we demonstrate that changes in sodium pump activity regulate locomotor networks in the spinal cord of neonatal mice. The sodium pump inhibitor, ouabain, increased the frequency and decreased the amplitude of drug-induced locomotor bursting, effects that were dependent on the presence of the neuromodulator dopamine. Conversely, activating the pump with the sodium ionophore monensin decreased burst frequency. When more natural locomotor output was evoked using dorsal-root stimulation, ouabain increased burst frequency and extended locomotor episode duration, whereas monensin slowed and shortened episodes. Decreasing the time between dorsal-root stimulation, and therefore interepisode interval, also ...
Arterial myocytes express α1-catalytic subunit isoform Na+ pumps (75-80% of total) which are ouabain resistant in rodents and high ouabain affinity α2-Na+ pumps. affect BP and vasoconstriction. α2SM-DN mice acquired elevated basal indicate BP (indicate Ondansetron (Zofran) BP by telemetry: 117 ± 4 vs. 106 ± 1 mmHg = 7/7 < 0.01) and enhanced BP replies to chronic ANG II infusion (240 ng·kg?1·min?1) and high (6%) NaCl. Many arterial Ca2+ transporters including Na+/Ca2+ exchanger 1 (NCX1) and sarcoplasmic reticulum and plasma membrane Ca2+ pushes [sarco(endo)plasmic reticulum Ondansetron (Zofran) Ca2+-ATPase 2 (SERCA2) and plasma membrane Ca2+-ATPase 1 (PMCA1)] had been also Continue Reading. ...
Showcase your wry biological / biochemical side with a dose of educational, scientific fun with any of these gifts featuring the sodium-potassium pump used in active transport along with the following saying: Stamp Out Imbalance Support Active Transport. Memorable gifts for all who know about the importance of osmoregulation ...
How can more molecules be transported into areas with high concentrations of the molecule? Cells often need to create concentration gradients across membranes. The concentration of a molecule is much higher on one side of the membrane than the other when a concentration gradient exists. In order to create or maintain a concentration gradient, individual molecules must be moved from an area of low concentration to an area of high concentration. This cannot occur through passive diffusion, in fact diffusion will cause the gradient to break down. Only active transport, which requires both specialized transport molecules and the expenditure of energy can drive molecules up a concentration gradient. The sodium potassium pump is an important and well understood example of active transport. View the animation below, then complete the quiz to test your knowledge of the concept.. ...
Levental, M and Tabakoff, B, Sodium-potassium-activated adenosine triphosphatase activity as a measure of neuronal membrane characteristics in ethanol-tolerant mice. (1980). Subject Strain Bibliography 1980. 2608 ...
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IntroductionOuabain (G-Strophanthin) inhibits the activity of Na-K activated adenosinetriphosphatase (ATPase) in in vitro and in vivo systems.1-3 The active tra
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Amaç: Bu çalışmada, basit horlama veya hafif derecede obstrüktif uyku apnesi (OUA) bulunan hastalarda yumuşak damağa radyofrekans doku ablasyonu (RFDA) uygulamasının erken dönemde horlama şiddeti, apne indeksi ve gündüz uykululuk haline etkileri değerlendirildi. Hastalar ve Yöntemler: Çalışmaya apnesiz basit horlaması olan 61, apne indeksi (Aİ) ≤5 ile basit horlaması olan 13 ve hafif derecede OUA (5 ...
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Ray P, Rolek D. The reversal by taurine of ouabain-induced arrhythmias and hypoglycemia. J Am Osteopath Assoc 1975;75(2):211. doi: .. Download citation file:. ...
1. Erythrocyte sodium, sodium transport (ouabain-sensitive efflux rate of sodium, oMosNa, and ouabain-sensitive efflux rate constant of sodium, oMosNa), sodium-potassium activated ouabain-sensitive adenosine triphosphatase (Na+, K+-ATPase) activity and [3H]ouabain-binding capacity were measured in 15 patients with chronic renal failure and in 10 healthy subjects.. 2. As a group, patients with chronic renal failure had a lower erythrocyte sodium and oMosNa compared with healthy subjects.. 3. When patients were divided according to their erythrocyte sodium (greater or less than 4 mmol/kg of cells), in the group of patients whose erythrocyte sodium was less than 4 mmol/kg of cells (group A) the oMosNa was higher than that in healthy subjects and the oMosNa, Na+, K+-ATPase activity and [3H]ouabain-binding capacity were the same as those in healthy subjects. In the subgroup of patients with renal failure whose erythrocyte sodium content was greater than 4 mmol/kg of cells (group B) the oMosNa was ...
Staphylococcus aureus is a notorious pathogen which often causes nosocomial and community attained infections. These infections steadily increased after evolving the resistance due to indecorous practice of antibiotics and now become a serious health issue. Ouabain is a Na+/K+-ATPase inhibitor that leads to increase the heart contraction in patients with congestive heart failure. In the present study, in vitro antimicrobial effect of ouabain together with aminoglycosides was determined against clinical and non-clinical S. aureus strains. Using checkerboard, Gentamycin uptake and biofilm assays, we analysed he interactions of ouabain with aminoglycosides. Ouabain induced the staphylocidal potency of aminoglycosides by remarkably reducing the MIC of gentamycin (GEN) by 16 (0.25 μg/mL), 8 folds (0.5 μg/mL) amikacin (AMK); and 16 folds (1.0 μg/mL) with kanamycin (KAN), compared to their individual doses. OBN severely reduced cell viability within 60 min with GEN (1 μg/mL), KAN (2 μg/mL) and 90 min with
The Na+ K+-ATPase pump maintains the gradient of a higher concentration of sodium extracellularly and a higher level of potassium intracellularly. The sodiumâ potassium pump is found in many cell (plasma) membranes. The sodium/potassium ATPase pump is essential to many physiological processes, and so targeting it with medication can be useful clinically. Explore the sodium potassium pump (Na+/K+ pump), with the Amoeba Sisters! Examples of â ¦ It also involves being conscious of every hint of flavoring in your food and how you cook your meals. In the kidneys, sodium and potassium have a reciprocal relationship. Na+-K+ pump in chronic renal failure. In a single cycle of the pump, three sodium ions are extruded from and two potassium â ¦ Powered by ATP, the pump moves sodium and potassium ions in opposite directions, each against its concentration gradient. Acting on the distal tubules, the hormone aldosterone triggers potassium excretion and resorption of sodium. Conversely, drugs which act ...
Sodium-potassium pump definition at Dictionary.com, a free online dictionary with pronunciation, synonyms and translation. Look it up now!
The plant-derived steroid, digoxin, a specific inhibitor of Na,K-ATPase, has been used for centuries in the treatment of heart disease. Recent studies demonstrate the presence of a digoxin analog, ouabain, in mammalian tissue, but its biological role has not been elucidated. Here, we show in renal epithelial cells that ouabain, in doses causing only partial Na,K-ATPase inhibition, acts as a biological inducer of regular, low-frequency intracellular calcium ([Ca2+](i)) oscillations that elicit activation of the transcription factor, NF-KB. Partial inhibition of Na,K-ATPase using low extracellular K+ and depolarization of cells did not have these effects. Incubation of cells in Ca2+-free media, inhibition of voltage-gated calcium channels, inositol triphosphate receptor antagonism, and redistribution of actin to a thick layer adjacent to the plasma membrane abolished [Ca2+](i) oscillations, indicating that they were caused by a concerted action of inositol triphosphate receptors and capacitative ...
Studies were performed on Na and K transport by red blood cells of the freshwater turtle under anaerobic and aerobic conditions. Although it had previously been assumed that cation transport in turtle red blood cells was dependent on respiration, the present data show greater Na efflux rates in N2 than in O2. However, ouabain inhibited Na transport by the same amount quantitatively in O2 and N2 gas phases. Thus there was no difference in ouabain-sensitive or pump Na transport rates. Na influx rates were higher in nitrogen than in air and potassium influx rates were not significantly different under aerobic and anaerobic conditions. Moreover in the absence of sodium in the bathing medium no difference between air and nitrogen could be discovered. Finally with ethacrynic acid plus ouabain there was an additional decrease in Na efflux but there was a persisting difference between air and nitrogen. These studies do not rule out the existence of a ouabain-insensitive ethacrynic acid-inhibitable ...
Canine cancers develop in immunocompetent environments and share many similar features observed in human cancers, such as long periods of latency for initiation, progression and development, metastasis, acquired drug resistance and recurrence. Osteosarcoma is a primary bone tumor that occurs in dogs. It is highly aggressive and metastatic in nature, and the therapeutic methods utilized to treat this primary canine tumor are very limited. We have successfully established canine osteosarcoma orthotopic xenograft models with NSG mice (from Jackson Lab) in vivo. Ouabain, known as Na+, K+ ATPase inhibitor, has been used for many years in human clinical applications for the treatment of congestive heart failure. Recently, ouabain has been investigated for potential anticancer properties. However, the therapeutic dose window is very narrow because the side effects of ouabain overdose can be lethal. In an attempt to mitigate such narrow therapeutic margin of safety, ouabain was formulated in lipid ...
Enhanced Na+/H+ exchange, measured as amiloride derivative-sensitive Na+ and H+ fluxes in cells with a preliminary acidified cytoplasm (Δμ H+-induced Na+/H+ exchange), is one of the most prominent intermediate phenotypes of altered vascular smooth muscle cell (VSMC) function in spontaneously hypertensive rats (SHR). Analysis of Na+/H+ exchange in F2 hybrids of SHR and normotensive rats seems to be the most appropriate approach in the search for the genetic determinants of abnormal activity of this carrier. However, the measurement of Δμ H+-induced Na+/H+ exchange is hardly appropriate for precise analysis of the carriers activity in VSMC derived from several hundred F2 hybrids. To overcome this problem, we compared the rate of 22Na influx under baseline conditions and in Na+-loaded (ouabain-treated) VSMC. The dose-dependency of the rate of Δμ H+-induced H+ efflux as well as of 22Na influx in control and ouabain-treated cells on ethylisopropylamiloride (EIPA) concentration were not ...
Voltage-gated Na+ channels are promising drug targets. Screening of large numbers of putative modulators, however, can be demanding and expensive. In this study, a simple, cheap, and robust assay to test the pharmacological modulation of Na+ channel function is presented. The assay makes use of the fact that the intracellular accumulation of Na+ ions can be cytotoxic. The toxicity of the Na+ channel activator veratridine in the presence of an inhibitor of the Na+/K+ ATPase (ouabain) in a Nav1.2a (rat brain IIA alpha) expressing cell line is assessed. Na+ channel blockers should reduce toxicity in this model. CHO cells which recombinantly expressed rat Nav1.2a subunits were seeded in 96-well plates, and cell survival was tested after 24 h incubation in medium containing veratridine and ouabain in the presence or absence of Na+ channel blockers. Propidium iodide fluorescence was used as toxicity readout. Veratridine (100 microM) or ouabain alone (500 microM) were not toxic to the cells. In the ...
In plants, an example of active transport being used is in the root hair cells of the plant, to move much-needed ions from the soil into the root of the plant. This also causes the water potential within the plant to decrease/become more negative, so more water moves in by osmosis, which is obviously beneficial to the plant. Animals also use active transport in a similar way, to move glucose and other important molecules, present from digested food, from the lumen of the small intestine, into the cells lining it, to be used for respiration, protein production and other processes. Another use of ATP in animals, that plants do not share, is within neurones. Nerve cells/neurones require ATP to provide energy to sodium-potassium pumps, which are embedded in the membrane of the cell body and within the nodes of Ranvier of the axons of (myelinated) neurones. These sodium-potassium pumps are required to achieve and maintain a resting potential before and after action potentials have been generated ...
Both high and low doses of CORT caused a modest depolarization of BLA neurons. Several mechanisms might account for this effect, including the inhibition of the sodium-potassium pump, of the resting K+ conductance, or of a voltage-dependent K+ current activated near rest. Because the membrane depolarization was observed without a significant increase in Rin in the low-dose CORT group, it is more likely that the effect of CORT is mediated by an inhibition of the sodium-potassium pump.. In contrast, the increase in Rin seen with the high dose of CORT probably involved the inhibition of one or more K+ currents such as the K+ leak or of a K+ conductance that is active near rest. Moreover, the fact that the high CORT dose markedly reduced spike-frequency adaptation suggests that CORT also inhibited one or more subtype(s) of voltage-gated or Ca2+-dependent K+ currents that are activated in the suprathreshold range of membrane potentials. In support of this hypothesis, previous studies have revealed ...
The possibility that alveolar macrophages and type-II cells can accumulate taurine was examined. The study involved measuring the taurine content of the cells, the rates of taurine uptake in the presence of a normal plasma concentration of taurine, the concentration dependence and kinetic parameters for the transport system, and the effects of metabolic inhibitors or ouabain on the transport proce
Does anyone leave the insulin pump site in for longer than 3 days or is that a no-no! I know that the sensor can be left in for longer but I did not...
The bottom line effect of this is to make cell effectively impermeable to NaCl. Gibbs Donnan equilibrium do not reached and cell does not swell inspite of the presence of protein ion ...
Our laboratory is interested in the physiological role of the Na,K-ATPase, particularly the function of the different alpha isoforms of this enzyme. The present...
The equilibrated of sodium and potassium from external to the bath governed by rate constant (0.09 ms and 0.05 ms). the governing equation of external included (unsteady term= diffusion flux + membrane flux+ ion access to/into FH space ...
Study: Sodium-to-potassium ratio a key to heart health Sodium-Saturated Diet Is a Threat for All Study Finds Sodium-Potassium Ratio Strongly Tied to
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Some of the chemicals in the plants are used to produce the drug ouabain, which was taken as a cardiac stimulant to treat heart ... ouabain), k-strophanthin, and e-strophanthin. As ordinarily administered, the drug acts on the heart before influencing any ...
"Resolution of the insect ouabain paradox". Proceedings of the National Academy of Sciences of the United States of America. 101 ...
Smith, LL (2004). "Oxygen, oxysterols, ouabain, and ozone: a cautionary tale". Free Radical Biology & Medicine. 37 (3): 318-24 ...
It is the aglycone of k-strophanthin, an analogue of ouabain. k-strophanthin is found in the ripe seeds of Strophanthus kombé ... Ouabain Digoxin Myocardial infarction Toxicity Detoxification Muscle contraction Cardiac glycosides 新药研发咨询中心. "k-Strophanthidin ... Bruno Kisch (New York City) noted that ouabain (strophanthin-G) has a positive ionotropic activity and faster onset than ... Song H, Karashima E, Hamlyn JM, Blaustein MP (March 2014). "Ouabain-digoxin antagonism in rat arteries and neurones". The ...
Its sap contains the deadly cardiotoxic glycoside ouabain. The sap is among the most commonly used in arrow poisons, including ...
23: 95-99 (1984). Binding of Cardiac Glycoside Ouabain to Dystrophic Erythrocyte Membranes. Pak. J. Pharmacol. 2: 37-42 (1985 ... 4 (1977). Foreign (International) Effect of ouabain upon erythrocyte membrane adenosine triphosphatase in Duchenne muscular ...
Ferrari, Patrizia; Ferrandi, Mara; Valentini, Giovanni; Bianchi, Giuseppe (2006). "Rostafuroxin: An ouabain antagonist that ... Ferrari, Patrizia (2010). "Rostafuroxin: An ouabain-inhibitor counteracting specific forms of hypertension". Biochimica et ... corrects renal and vascular Na+-K+- ATPase alterations in ouabain and adducin-dependent hypertension". American Journal of ...
Post asked whether the enzyme was inhibited by ouabain. At this stage Skou was unaware that ouabain inhibited the pump, but he ... Ouabain did indeed inhibit the enzyme, thus establishing a link between the enzyme and the sodium-potassium pump. Following the ... and in his research he had made use of a substance called ouabain (or g-strophanthin) which had recently been shown to inhibit ...
Berova, Nina (1993). "Physicochemical characterization of a ouabain isomer isolated from bovine hypothalamus". PNAS. 90 (17): ...
Pitts BJ, Meyerson LR (1981). "Inhibition of Na,K-ATPase Activity and Ouabain Binding by Sanguinarine". Drug Development ...
However, a high dose of ouabain can lead to ventricular tachycardia. C. Andreasen, et al. (2006) Mosby Elsevier, Mosby's ... Third degree AV block can be treated with Cilostazol which acts to increase Ventricular escape rate Ouabain infusion decreases ...
This plant contains ouabain, which is a cardiac glycoside, oleander, and milkweeds. Poisoned arrows are also still used in the ...
Habermann E, Chhatwal GS (1 May 1982). "Ouabain inhibits the increase due to palytoxin of cation permeability of erythrocytes ... where it interacts with the natural binding site of ouabain with very high affinity. Na+/K+-ATPase is a transmembrane protein, ...
Ouabain. ReferencesEdit. *^ OED *^ a b c d e "Digoxin". The American Society of Health-System Pharmacists. Archived from the ...
... is observed for lower ouabain concentrations, dystonia is observed at higher ouabain concentrations. Antibodies against ... Indeed, an ouabain block of Na+ -K+ pumps in the cerebellum of a live mouse results in it displaying ataxia and dystonia. ...
May 2020). "Identification of Atovaquone, Ouabain and Mebendazole as FDA-Approved Drugs Targeting SARS-CoV-2". chemRxiv ( ...
"Suppression of ouabain-insensitive K-ATPase activity in rabbit nephron segments during chronic hyperkalemia". Renal Physiology ...
1999). "Ouabain-sensitive H,K-ATPase: tissue-specific expression of the mammalian genes encoding the catalytic alpha subunit". ... This gene encodes a catalytic subunit of the ouabain-sensitive H+/K+ -ATPase that catalyzes the hydrolysis of ATP coupled with ... Sverdlov VE, Kostina MB, Modyanov NN (1997). "Genomic organization of the human ATP1AL1 gene encoding a ouabain-sensitive H,K- ... "Genomic organization of the human ATP1AL1 gene encoding a ouabain-sensitive H,K-ATPase". Genomics. 32 (3): 317-27. doi:10.1006/ ...
"Reducing the Late Sodium Current Improves Cardiac Function during Sodium Pump Inhibition by Ouabain". Journal of Pharmacology ...
... resembles very much other glycosides like ouabain and digoxin but has less effect than digoxin. It is however, just ... like its derivate oleandrigenin, a more potent glycoside than ouabain. Oleandrin and its derivate oleandrigenin are formed in ...
Furthermore, the presence of a sugar group in ouabain and the absence of one in arenobufagin suggests that it may me more ... along with ouabain. It has been suggested that uncharged and non-polar amino acids may participate in the binding of ... lipophilic that ouabain and as such form a more stable complex with the Na+-K+ pump. Besides its effects on the Na+-K+ pump, ...
"Sodium-dependence and ouabain-sensitivity of the synthesis of dopamine in renal tissues of the rat". Br. J. Pharmacol. 105 (4 ...
They tipped their arrows with ouabain, a poison which caused death by cardiac arrest or respiratory failure. The Maya homeland ...
NEM activates ouabain-insensitive Cl-dependent K efflux in low K sheep and goat red blood cells. This discovery contributed to ...
The drugs were later publicly identified as digitalis and ouabain, both of which are cardenolide-type cardiac glycosides.) ...
Ebner, F; Reiter, M (June 1979). "The alteration by propranolol of the inotropic and bathmotropic effects of dihydro-ouabain on ...
"Differential effects of calcium channel antagonists on tityustoxin and ouabain-induced release of [3H]acetylcholine from brain ...
Such systems include the HPRT assay for resistance to 8-azaguanine or 6-thioguanine, and ouabain-resistance (OUA) assay. Rat ...
The consequence of the drugs actions are irreversible cell swelling and lysis (e.g. ouabain at very high doses). List of ...
Ouabain is a highly toxic compound with a LD50 of 5 mg/kg when administered orally to rodents. However, ouabain has a low ... Although ouabain is no longer approved for use in the USA, in France and Germany, intravenous ouabain has a long history in the ... Ouabain was seen as a possible treatment for certain cardiac conditions. Strophanthidin "ouabain" in the World English ... Hence, it was suggested that some assays for endogenous ouabain detected other compounds or failed to detect ouabain at all. ...
The cerebral cortex to which 10−3M ouabain was applied showed a spongy... ... Ouabain, a specific inhibitor of active cation transport across cell membrane, was applied topically to brain surface of cats. ... There was a marked increase in sodium with a slight increase of water in the cortex treated with 10−3 M ouabain. No evidence of ... Ouabain, a specific inhibitor of active cation transport across cell membrane, was applied topically to brain surface of cats. ...
Ouabain is not detectable in human plasma.. L K Lewis, T G Yandle, J G Lewis, A M Richards, G B Pidgeon, R J Kaaja, M G ... Ouabain is not detectable in human plasma.. L K Lewis, T G Yandle, J G Lewis, A M Richards, G B Pidgeon, R J Kaaja and M G ... Ouabain is not detectable in human plasma.. L K Lewis, T G Yandle, J G Lewis, A M Richards, G B Pidgeon, R J Kaaja and M G ... there was no support for the immunoreactive material in the latter samples being ouabain. We propose that if ouabain is present ...
... on severity of ouabain-induced cardiac arrhythmias and the possible involvement of serum catecholamines were investigated in ... The effects of the opioid-type stressor, immobilization, on severity of ouabain-induced cardiac arrhythmias and the possible ...
The results showed that ouabain-induced damage in vitro was dose and time dependent. 500 μM ouabain and 1000 μM ouabain were ... In prior studies, locally administering ouabain via round window membrane demonstrated that the ototoxic effects of ouabain in ... Ouabain-Induced Apoptosis in Cochlear Hair Cells and Spiral Ganglion Neurons In Vitro. Yong Fu,1,2 Dalian Ding,2 Lei Wei,2 ... Ouabain is a common tool to explore the pathophysiological changes in adult mammalian cochlea in vivo. ...
A) Ouabain-unbound state. (B) Low-affinity ouabain-bound state. (C) Hypothetical one for high-affinity ouabain-bound state. ... Details of the ouabain binding site. (A) Crystal structure of the low-affinity ouabain (OBN)-bound state. (B) Hypothetical one ... A) Superimposition of Cα traces of Na+,K+-ATPase from shark rectal gland in ouabain-bound (yellow) and ouabain-unbound (cyan) ... A) Superimposition of Cα traces of Na+,K+-ATPase in ouabain-bound (yellow) and ouabain-unbound (cyan) forms, viewed parallel to ...
W. Schoner, N. Bauer, J. Müller-Ehmsen et al., "Ouabain as a mammalian hormone," Annals of the New York Academy of Sciences, ... L. de Sá Lima, E. M. Kawamoto, C. D. Munhoz et al., "Ouabain activates NFκB through an NMDA signaling pathway in cultured ... L. S. de Paiva, K. M. D. Costa, F. B. D. Canto et al., "Modulation of mature B cells in mice following treatment with ouabain ... J. M. Hamlyn and M. P. Blaustein, "Salt sensitivity, endogenous ouabain and hypertension," Current Opinion in Nephrology and ...
After competition with ouabain within the extract bound anti-ouabain-IgG was detected with biotinylated anti-sheep-IgG and ... anti-ouabain antibodies from sheep were allowed to bind to ouabain-C6-trypsin conjugate (0.1 μg/well) attached to a microtiter ... 1.7 nmol/L ouabain equivalents; P,0.05). In parallel, endogenous ouabain immunoreactivity (OLC) increased 36-fold from 2.5±0.5 ... 22 A transient increase of endogenous ouabain may not lead to inhibition of the pump, although a rapidly dissociable ouabain-Na ...
We investigated uptake of ouabain into isolated rat hepatocytes and release of ouabain from preloaded hepatocytes, thus ... Ouabain was accumulated within hepatocytes 170-fold. The predominant fraction of intracellular ouabain beeing unbound. ... We investigated uptake of ouabain into isolated rat hepatocytes and release of ouabain from preloaded hepatocytes, thus ... Uptake of ouabain was saturable (Km=263±61μM, V=3.3±1.0 nmol/mg prot.×min), energy-dependent, sensitive to dinitrofluorobenzene ...
Injection of ouabain, a potent and selective inhibitor of Na+/K+ ATPase, into the rat striatum and substantia nigra caused a ... The sodium-potassium ATPase inhibitor ouabain is neurotoxic in the rat substantia nigra and striatum.. Lees GJ1, Leong W. ...
Acute Effects of Intravenous Ouabain in Healthy Volunteers. G. B. Pidgeon, A. M. Richards, M. G. Nicholls, L. K. Lewis, T. G. ... Acute Effects of Intravenous Ouabain in Healthy Volunteers. G. B. Pidgeon, A. M. Richards, M. G. Nicholls, L. K. Lewis, T. G. ... 4. Ouabain injection was associated with a fall in plasma angiotensin II levels and a rise in plasma adrenaline levels, but ... Acute Effects of Intravenous Ouabain in Healthy Volunteers Message Subject (Your Name) has forwarded a page to you from ...
Since endogenous ouabain (EO) is a neuro-endocrine hormone involved in renal damage, we evaluated the role of EO in renal ... We studied five groups of rats: (1) normal; (2) infused for eight weeks with ouabain (30 µg/kg/day, OHR) or (3) saline; (4) ... Nephrin progressively decreased and KIM-1 mRNA increased starting from 24 h. Ouabain infusion (group 2) increased blood ... ouabain; or (5) saline-infused rats orally treated with 100 µg/kg/day rostafuroxin for four weeks. In group 1, 2-3 h after IRI ...
These three mAbs are distinguished from existing Abs to ouabain and digoxin by their specificity for ouabain and lack of cross- ... 4 Abbreviations in this paper: Oua, ouabain; Dig, digoxin; BGG, bovine γ-globulin; OLC, ouabain-like compound(s); HSA, human ... Our attempts to produce ouabain-specific mAbs were unsuccessful when ouabain was coupled to exogenous proteins such as bovine γ ... Is the circulating ouabain-like compound ouabain?. Am. J. Hyperten. 7: 647. ...
Effects of Ouabain on the Left Ventricular Response to Exercise in Patients with Angina Pectoris. D. LUKE GLANCY, LAWRENCE M. ... Ouabain improved LV performance at rest in only a minority of patients, but during the stress of exercise, LV hemodynamics were ... The response of the left ventricle to a level of supine bicycle exercise that induced angina, and the effects of ouabain (0.01 ... Before administration of ouabain, exercise was associated with depressed pumping performance of the left ventricle in four ...
The ouabain effects on Wt1 and Pax2 mRNA were abolished in kidneys simultaneously exposed to ouabain and helenalin (Fig. 4c,d). ... Exposure to ouabain increases calcium activity. Exposure of embryonic kidneys to ouabain caused a significant increase in the ... This daily dose of ouabain has previously been shown to result in plasma levels of ouabain around 1 nM43 and to have little ... In ouabain-exposed kidneys, no adverse effects of serum deprivation were observed. Proof of principle that ouabain rescues ...
A 1 μl syringe was lowered into the left striatum to a depth of 4.0 mm (Dijkhuizen et al., 1996). Ouabain (0.5 μl 1 mm; n= 30; ... Three adjacent coronal T2 maps of neonatal rat brain 7 d after ouabain injection. a,No treatment; b, THC treatment; c, THC + ... Three adjacent coronal ADC maps of neonatal rat brain 15 min after ouabain injection. a, No treatment;b, THC treatment; c, THC ... The diffusion-weighted MRI data acquired 15 min after the injection of ouabain showed that activation of the CB1receptor by Δ9- ...
Myocardial uptake of ouabain in intact dog and man. Message Subject (Your Name) has forwarded a page to you from Journal of ... Myocardial uptake of ouabain in intact dog and man.. R Selden and W A Neill ... Myocardial uptake of ouabain in intact dog and man.. R Selden and W A Neill ... Myocardial uptake of ouabain in intact dog and man.. R Selden and W A Neill ...
Blockade of ouabain-induced [C-a2+](i) oscillations prevented activation of NF-kappaB. The results demonstrate a new mechanism ... Here, we show in renal epithelial cells that ouabain, in doses causing only partial Na,K-ATPase inhibition, acts as a ... Ouabain, a steroid hormone that signals with slow calcium oscillations. Aizman, O. ... Recent studies demonstrate the presence of a digoxin analog, ouabain, in mammalian tissue, but its biological role has not been ...
Considering that Ouabain (OUA) may prevent the apoptosis process, in this study we evaluated if OUA is able to avoid the damage ... Ouabain Protects Human Renal Cells against the Cytotoxic Effects of Shiga Toxin Type 2 and Subtilase Cytotoxin. María M. Amaral ... Considering that Ouabain (OUA) may prevent the apoptosis process, in this study we evaluated if OUA is able to avoid the damage ... "Ouabain Protects Human Renal Cells against the Cytotoxic Effects of Shiga Toxin Type 2 and Subtilase Cytotoxin." Toxins 9, no. ...
OUABAIN. C29 H44 O12. LPMXVESGRSUGHW-HBYQJFLCSA-N. Ligand Interaction. ... Crystal structure of the high affinity complex between ouabain and the E2P form of the sodium-potassium pump. *DOI: 10.2210/ ... Most importantly, the A domain has rotated in response to phosphorylation and αM1-2 move towards the ouabain molecule, ... K+-ATPase in its phosphorylated form stabilized by high affinity binding of the cardiotonic steroid ouabain. The steroid binds ...
Is the circulating ouabain-like compound ouabain? Am J Hypertens.. 1994;7:647-650. ... More recently, Doris et al29 did find ouabain immunoreactive material but no authentic ouabain in the plasma of patients with ... OLA is expressed as nanograms of ouabain equivalents per milliliter plasma and micrograms of ouabain equivalents per gram ... Brain Ouabain Mediates Sympathetic Hyperactivity in Congestive Heart Failure. Frans H. H. Leenen, Bing S. Huang, Huilian Yu, ...
Ouabain increased dP/dt similarly by 600 ± 90 mm Hg/sec, but did not change heart rate or mean arterial pressure significantly ... SUMMARY The effects of ouabain, 20 μg/kg, and isoproterenol, 0.03 μg/kg/min, were compared in conscious dogs with acute ... In contrast to isoproterenol, ouabain increased flow 46 ± 9.2%, P < 0.01, systolic segment shortening 0.35 ± 0.10 mm, P < 0.01 ... In conclusion, equi-inotropic doses of ouabain and isoproterenol induced opposite effects on the mechanical function, ECGs and ...
Ray P, Rolek D. The reversal by taurine of ouabain-induced arrhythmias and hypoglycemia. J Am Osteopath Assoc 1975;75(2):211. ... The reversal by taurine of ouabain-induced arrhythmias and hypoglycemia You will receive an email whenever this article is ... The reversal by taurine of ouabain-induced arrhythmias and hypoglycemia. The Journal of the American Osteopathic Association, ...
Interaction of ouabain and progesterone on induction of bull sperm capacitation.. The endogenous steroid hormone ouabain ... Ouabain and digoxin are classical inhibitors of the Na,K-ATPase. In addition to their conventional uses as therapeutic agents ... The cardenolide ouabain suppresses coronaviral replication via augmenting a Na/K-ATPase-dependent PI3K_PDK1 axis signaling.. 08 ... Ouabain, a representative cardenolide, was found to potently diminish TGEV titers and inhibit the TGEV-induced production of IL ...
INFLUENCE OF NOREPINEPHRINE AND OUABAIN ON CARDIAC MUSCLE MECHANICS DURING HYPOTHERMIA. Joanne I. Moore and Marion deV. Cotten ... Norepinephrine after ouabain only decreased contractile force, dT/dt, ISF, time to peak force and duration of the cardiac cycle ... INFLUENCE OF NOREPINEPHRINE AND OUABAIN ON CARDIAC MUSCLE MECHANICS DURING HYPOTHERMIA. Joanne I. Moore and Marion deV. Cotten ... INFLUENCE OF NOREPINEPHRINE AND OUABAIN ON CARDIAC MUSCLE MECHANICS DURING HYPOTHERMIA. Joanne I. Moore and Marion deV. Cotten ...
Endogenous Ouabain and Its Binding Globulin: Effects of Physical Exercise and Study on the Globulins Tissue Distribution * * ... Exogenous Ouabain Is Accumulated in the Adrenals and Mimics the Kinetics of Endogenous Digitalis-like Factor in Rats KITANO ... Secretion of endogenous ouabain from bovine adrenal cells: role of zona glomerulosa and zona fasciculata LAREDO J. ... Ouabain, that has been isolated from bovine adrenals and hypothalamus, is a new cardiotonic steroid hormone, which is either ...
Antiarrhythmic activity against ouabain-induced arrhythmia in guinea pig right ventricle assessed as reversal of cardiac ...
Actions of bufalin and cinobufotalin, two bufadienolides respectively more active and less active than ouabain, on ouabain ... Actions of Ouabain and of Diuretics on Na+-K+ Exchanges in Skeletal and Heart Muscle S. S. Bhattacharya S. S. Bhattacharya ... S. S. Bhattacharya, A. Jackson, C. T. G. Flear; Actions of Ouabain and of Diuretics on Na+-K+ Exchanges in Skeletal and Heart ... Differences in ouabain-induced natriuresis between isolated kidneys of Milan hypertensive and normotensive rats Clin Sci (Lond) ...
The safety and delivery efficiency of ouabain in vivo was also improved, suggesting a therapeutic application of ouabain double ... Ouabain, known as Na+, K+ ATPase inhibitor, has been used for many years in human clinical applications for the treatment of ... Recently, ouabain has been investigated for potential anticancer properties. However, the therapeutic dose window is very ... Antitumor properties of ouabain in lipid double emulsion in orthotopic canine osteosarcoma xenografted mouse model. [abstract ...
Schoner W, Bauer N, Mülle-Ehmsen J et al (2003) Ouabain as a mammalian hormone. Ann N Y Acad Sci 986:678-684PubMedCrossRef ... Scheiner-Bobis G, Schoner W (2001) A fresh facet for ouabain action. Nat Med 7:1288-1289PubMedCrossRefGoogle Scholar ... Ouabain BDNF Retina Neuronal survival Synergistic effect Aline Araujo dos Santos and Elizabeth Giestal-de-Araujo have ... Ouabain is a steroid that binds to the Na+/K+ ATPase, inducing the activation of several intracellular signaling pathways. ...
  • A number of analytical techniques led to the conclusion that this circulating molecule was ouabain and that humans were producing it as an endogenous hormone. (wikipedia.org)
  • A large portion of the scientific community agreed that this inhibitor was endogenous ouabain and that there was strong evidence to indicate that it was synthesized in the adrenal gland. (wikipedia.org)
  • One early speculative interpretation of the analytical data led to the proposal that endogenous ouabain may have been the 11 epimer, i.e., an isomer of plant ouabain. (wikipedia.org)
  • Critically, the primary observations concerning the identification of ouabain in mammals were repeated and confirmed using a variety of tissue sources on three different continents with advanced analytical methods as summarized elsewhere Despite widespread analytical confirmation, some questioned whether or not this endogenous substance is ouabain. (wikipedia.org)
  • Hence, it was suggested that some assays for endogenous ouabain detected other compounds or failed to detect ouabain at all. (wikipedia.org)
  • Yet another argument against the existence of endogenous ouabain was the lack of effect of rostafuroxin (a first generation ouabain receptor antagonist) on blood pressure in an unselected population of hypertensive patients. (wikipedia.org)
  • Endogenous ouabain and its binding globulin: effects of physical exercise and study on the globulin's tissue distribution," Hypertension Research , vol. 23, supplement, pp. (hindawi.com)
  • J. M. Hamlyn and M. P. Blaustein, "Salt sensitivity, endogenous ouabain and hypertension," Current Opinion in Nephrology and Hypertension , vol. 22, pp. 51-58, 2013. (hindawi.com)
  • Ouabain or its isomer has been isolated from blood, adrenals, and hypothalamus 1-3 as one of the endogenous cardiac glycosides circulating in blood plasma. (ahajournals.org)
  • If so, physical exercise associated with the increase in epinephrine and norepinephrine should consequently increase endogenous ouabain. (ahajournals.org)
  • Here, we investigated the effect of physical exercise on the endogenous ouabain plasma concentration in several experimental settings as well as the influence of β-blockade and angiotensin-converting enzyme (ACE) inhibition. (ahajournals.org)
  • Since endogenous ouabain (EO) is a neuro-endocrine hormone involved in renal damage, we evaluated the role of EO in renal ischemia-reperfusion injury (IRI). (mdpi.com)
  • Several reports have also pointed to the existence of endogenous ouabain- and digoxin-like compounds, but their precise roles in mammalian physiology and various disorders of the circulation are not clear. (jimmunol.org)
  • In an attempt to produce specific Abs for the purification and identification of endogenous ouabain-like compounds, somatic cell fusion was used to produce mAbs specific for ouabain. (jimmunol.org)
  • The endogenous steroid hormone ouabain induces capacitation of bull sperm acting through its receptor Na/K-ATPase on the sperm plasma membrane. (bioportfolio.com)
  • It may affect the action of endogenous ouabain on heart and circulation, but the physiological function of this protein is unclear. (nii.ac.jp)
  • may affect blood concentrations of endogenous ouabain and which function the cardiotonic binding protein may have in modulating ouabain effects, the effect of physical exercise on endogenous ouabain was studied and the tissue distribution of its binding protein was investigated. (nii.ac.jp)
  • We found that endogenous ouabain changes rapidly in blood upon physical exercise and behaves like expected for a hormone of circulation. (nii.ac.jp)
  • Endogenous concentrations of ouabain act as a cofactor to stimulate fluid secretion and cyst growth of in vitro ADPKD models via cAMP and EGFR-Src-MEK pathways. (semanticscholar.org)
  • Signaling mechanisms that link salt retention to hypertension: endogenous ouabain, the Na(+) pump, the Na(+)/Ca(2+) exchanger and TRPC proteins. (semanticscholar.org)
  • Physiological roles of endogenous ouabain in normal rats. (semanticscholar.org)
  • The effect of ouabain and an endogenous ouabain-like compound (OLC) was studied on (3H)noradrenaline ((3H)NA) release from rabbit pulmonary artery. (elsevier.com)
  • To investigate renal sodium handling of ouabain -hypertensive rats and the role of renal sodium handling in pathogenesis of hypertension using endogenous trace lithium as a marker of proximal sodium reabsorption. (bvsalud.org)
  • In the present study we investigated the membrane events and the ionic processes which mediate the stimulatory effect of ouabain on the release of endogenous dopamine (DA) and previously taken-up (3H)DA release from rat hypothalamic tuberoinfundibular dopaminergic (TIDA) neurons. (osti.gov)
  • Ouabain (0.1-1 mM) dose-dependently stimulated endogenous DA and newly taken-up (3H)DA release. (osti.gov)
  • Moreover, other adrenocortical steroids, including endogenous ouabain can stimulate tissue damage. (elsevier.com)
  • As endogenous ouabain is often elevated in chronically Na-loaded states, is a vasoconstrictor, raises blood pressure (BP), and also promotes tissue fibrosis, we investigated the extent to which plasma Aldo and endogenous ouabain were coelevated among naïve hypertensive patients (NHP). (elsevier.com)
  • These data suggest that very small doses of ouabain, which might produce nanomolar plasma concentrations, enhance pressor reactivity in DOCA-salt hypertensive rats, supporting the idea that endogenous ouabain may contribute to the increase and maintenance of vascular tone in hypertension. (ufes.br)
  • Ouabain is a cardiac glycoside that acts by inhibiting the Na⁺/K⁺-ATPase sodium-potassium ion pump (but it is not selective). (wikipedia.org)
  • Here we describe a crystal structure of Na + ,K + -ATPase with bound ouabain, a representative cardiac glycoside, at 2.8 Å resolution in a state analogous to E2·2K + ·Pi. (pnas.org)
  • However, how ouabain binds to the ATPase is still elusive. (pnas.org)
  • Although it is established that it binds with high affinity to the ATPase in E2P-like states [ K d ≈0.7 nM for shark enzyme used here ( 13 )], the ATPase-ouabain complexes formed by the forward (i.e., in the presence of ATP + Na + + Mg 2+ ) and backward (i.e., in the presence of Pi + Mg 2+ ) reactions have different properties ( 14 ). (pnas.org)
  • Particularly complicated is the effect of K + , which accelerates the dephosphorylation of the ATPase and thereby antagonizes ouabain binding. (pnas.org)
  • Yet, ouabain binds to the ATPase even in the E2·2K + state, although at a mM affinity, and stabilizes the occlusion of K + ( 15 ). (pnas.org)
  • Here we describe a 2.8 Å resolution crystal structure of the Na + ,K + -ATPase in a state analogous to E2·2K + ·Pi with bound ouabain and compare it to the unbound form ( 16 ) and the corresponding form of Ca 2+ -ATPase ( 17 ). (pnas.org)
  • Thus, the crystal structure supports the proposal that the M5 and M6 helices are the main binding site ( 2 , 9 ), but urges reconsideration of previous studies that have placed ouabain on the extracellular surface of the ATPase α-subunit. (pnas.org)
  • The sodium-potassium ATPase inhibitor ouabain is neurotoxic in the rat substantia nigra and striatum. (nih.gov)
  • Injection of ouabain, a potent and selective inhibitor of Na+/K+ ATPase, into the rat striatum and substantia nigra caused a selective neuronal loss in up to 70% of the lesioned area. (nih.gov)
  • We measured plasma and renal EO variations and markers of glomerular and tubular damage (nephrin, KIM-1, Kidney-Injury-Molecule-1, α1 Na-K ATPase) and the protective effect of the ouabain inhibitor, rostafuroxin. (mdpi.com)
  • Using explanted rat embryonic kidneys, we found that ouabain, the Na,K-ATPase ligand, triggers a calcium-nuclear factor-κB signal, which protects kidney development from adverse effects of malnutrition. (pubmedcentralcanada.ca)
  • Spontaneous calcium waves were observed in the majority of cells, and both acute and chronic exposure to ouabain, in concentrations that had little or no effect on Na,K-ATPase pump function, were found to increase the number of calcium waves. (pubmedcentralcanada.ca)
  • Here, we show in a longitudinal magnetic resonance imaging study that Δ 9 -tetrahydrocannabinol (Δ 9 -THC), the main active compound in marijuana, reduces neuronal injury in neonatal rats injected intracerebrally with the Na + /K + -ATPase inhibitor ouabain to elicit excitotoxicity. (jneurosci.org)
  • Here, we show in renal epithelial cells that ouabain, in doses causing only partial Na,K-ATPase inhibition, acts as a biological inducer of regular, low-frequency intracellular calcium ([Ca2+](i)) oscillations that elicit activation of the transcription factor, NF-KB. (diva-portal.org)
  • Here we describe the 4.6 Å resolution crystal structure of the pig kidney Na+,K+-ATPase in its phosphorylated form stabilized by high affinity binding of the cardiotonic steroid ouabain. (rcsb.org)
  • In recent years, evidence has been increasing that this inhibitor of the Na + ,K + -ATPase enzyme may be a steroid closely related to ouabain. (ahajournals.org)
  • The cardenolide ouabain suppresses coronaviral replication via augmenting a Na/K-ATPase-dependent PI3K_PDK1 axis signaling. (bioportfolio.com)
  • By pharmacological inhibition and gene silencing, we demonstrated that PI3K_PDK1_RSK2 signaling was induced in TGEV-infected ST cells, and ouabain imparted a degree of anti-TGEV activity via further augmentation of this existing PI3K_PDK1 axis signaling, in a manner dependent upon its association with the Na/K-ATPase. (bioportfolio.com)
  • Ouabain and digoxin are classical inhibitors of the Na,K-ATPase. (bioportfolio.com)
  • DR region specific antibody ameliorated but ouabain worsened renal injury in nephrectomized rats through regulating Na,K-ATPase mediated signaling pathways. (bioportfolio.com)
  • Ouabain, known as Na+, K+ ATPase inhibitor, has been used for many years in human clinical applications for the treatment of congestive heart failure. (aacrjournals.org)
  • Ouabain is a steroid that binds to the Na + /K + ATPase, inducing the activation of several intracellular signaling pathways. (springer.com)
  • 3H]ouabain localization of Na-K ATPase in the epithelium of rabbit ciliary body pars plicata. (arvojournals.org)
  • The secretion of the aqueous humor has been proposed to occur as the result of active Na+ transport by a ouabain-sensitive Na-K ATPase. (arvojournals.org)
  • New insights into the regulation of Na+,K+-ATPase by ouabain. (semanticscholar.org)
  • Human Na,K-ATPase α1 and α2 isoforms exhibit a high ouabain affinity but significantly differ in the ouabain association and dissociation rates. (aspetjournals.org)
  • Thus, we have identified new structural determinants in the Na,K-ATPase α-subunit that are involved in ouabain binding and probably control, in an α isoform-specific way, the access and release of ouabain to and from its binding site. (aspetjournals.org)
  • Compounds such as digoxin, digitoxin, and ouabain are plant-derived steroids that bind to Na,K-ATPase with high selectivity and inhibit its transport activity. (aspetjournals.org)
  • A comparison of ouabain-treated, cold-inhibited, and neonatal corneas, as well as consideration of electron histochemical studies on ATPase distribution in the cornea, suggests that a common mechanism may underlie the morphological changes and that the pinocytotic transport system in the corneal endothelium may be dependent on an electrolyte transport system for proper function. (arvojournals.org)
  • These results are consistent with the concept that in neoplasia a primary event is stimulation of Na+/H+ exchange, which leads to secondary stimulation of the ouabain-sensitive Na+/K+ ATPase pump. (lancs.ac.uk)
  • 3H]Ouabain binding and Na+, K+-ATPase in resealed human red cell ghosts. (rupress.org)
  • Ouabain activates the Na-K-ATPase signalosome to induce autosomal dominant polycystic kidney disease cell proliferation. (semanticscholar.org)
  • 1. The present study has examined the influence of sodium chloride (0-160 mM) and ouabain (100 and 500 microM), an inhibitor of the enzyme Na(+)-K+ ATPase, on the synthesis of dopamine in slices of rat renal cortex loaded with exogenous L-dihydroxyphenylalanine (L-DOPA). (mysciencework.com)
  • that these progenitor cells differ in their response to ouabain and finally, that ouabain at concentrations below that (10 -6 M) considered necessary to inhibit membrane-bound Na + K + ATPase can influence the proliferation of mouse hematopoietic progenitor cells. (elsevier.com)
  • With this in mind, Georg and team sought to modify ouabain so that it would target Na,K-ATPase α4, while steering clear of Na,K-ATPase subunits in the heart. (medicalnewstoday.com)
  • The scientists tested a number of different ouabain modifications for the new study, and they found that by removing a sugar group and the lactone group from the compound and replacing the latter with a triazole group, they created a compound that could selectively target the Na,K-ATPase α4 subunit in sperm cells. (medicalnewstoday.com)
  • On testing this newly created form of ouabain in male rats, they found that it was able to bind to the Na,K-ATPase α4 subunit in sperm and reduce their motility, or ability to swim. (medicalnewstoday.com)
  • since Na,K-ATPase α4 subunits are only present on mature sperm cells, any sperm cells produced after treatment with the ouabain-derived compound should not be affected. (medicalnewstoday.com)
  • Ouabain decreases sarco(endo)plasmic reticulum calcium ATPase activity" by David J. Kennedy, Sandeep Vetteth et al. (marshall.edu)
  • The studies described in this report focus on defining the effects of ouabain signaling on sarcoplasmic reticulum Ca 2+ -ATPase function. (marshall.edu)
  • Analysis of sarcoplasmic reticulum Ca 2+ -ATPase protein revealed increases in both the oxidation and nitrotyrosine content in the ouabain-treated hearts. (marshall.edu)
  • Liquid chromatography-mass spectrometric analysis confirmed that the sarcoplasmic reticulum Ca 2+ -ATPase protein from ouabain-treated hearts had modifications consistent with oxidative and nitrosative stress. (marshall.edu)
  • These data suggest that ouabain induces oxidative changes of the sarcoplasmic reticulum Ca 2+ -ATPase structure and function that may, in turn, produce some of the associated changes in Ca 2+ cycling and physiological function. (marshall.edu)
  • Previous research shows Ouabain (OUA) to bind Na, K-ATPase, thereby triggering a number of signaling pathways, including the transcription factors NFᴋB and CREB. (trajettoria.com)
  • Previous research shows Ouabain (OUA) to bind Na, K-ATPase, thereby triggering a. (trajettoria.com)
  • Neonatal pretreatment with ouabain depressed myocardial ouabain binding, but enhanced the Na + K + -ATPase activity. (elsevier.com)
  • Here we describe a crystal structure of the phosphorylated pig kidney Na+,K+-ATPase in complex with the CTS representative ouabain, extending to 3.4 Å resolution. (forskningsdatabasen.dk)
  • We treated bovine pulmonary artery smooth muscle cells with a low dose of ouabain (10 nM) and determined [Ca2+]i in the cells by fluorometric assay using fura2-AM, m-calpain activity by fluorometric assay using SLLVY-AMC as the substrate, PKC activity using an assay kit and assay of Na+/K+ATPase activity spectrophotometrically. (bvsalud.org)
  • Na-K-ATPase was inhibited using 1 mM ouabain or siRNA for Na-K-ATPase-α1 in IEC-18 cells. (nih.gov)
  • effect of ouabain on Na-K-ATPase activity in IEC-18 cells. (nih.gov)
  • Treatment of IEC-18 cells for 1 h with 1 mM ouabain significantly inhibited Na-K-ATPase activity compared with control. (nih.gov)
  • Sodium-dependent transporters are inhibited indirectly by the Na-K-ATPase inhibitor ouabain. (elsevier.com)
  • Introduction Ouabain (G-Strophanthin) inhibits the activity of Na-K activated adenosinetriphosphatase (ATPase) in in vitro and in vivo systems. (jamanetwork.com)
  • 4,5 Simon and Bonting 6 have shown Na-K activated ATPase to be present in the ciliary body and to be inhibited by ouabain. (jamanetwork.com)
  • This study investigated the effect of ouabain, an inhibitor of NA(+) and K(+) ATPase, on consolidation and reconsolidation of a passive avoidance learning task in the day-old chick. (edu.au)
  • In LLC-PK1 cells, the Na/K-ATPase-α1 and carcinoembryonic antigen cell adhesion molecule 1, a plasma membrane protein that promotes receptor-mediated endocytosis, colocalized in the plasma membranes and translocated to the intracellular region in response to ouabain. (marshall.edu)
  • When caveolin or Na/K-ATPase-α1 expression was knocked down with small interfering RNA, insulin but not ouabain induced carcinoembryonic antigen cell adhesion molecule 1, insulin receptor-β, and epidermal growth factor receptor endocytosis. (marshall.edu)
  • We conclude that plasma DLF is associated with reduced ouabain sensitive sodium transport and increased intracellular sodium concentration, possibly due to changes in the number of or the competition for the Na + K + ATPase sites. (elsevier.com)
  • Ouabain is a selective Na+, K+-ATPase inhibitor. (adooq.com)
  • A novel Src inhibitor that targets the Na/K-ATPase/Src receptor complex and antagonizes ouabain-induced protein kinase cascades and uses thereof are disclosed. (utoledo.edu)
  • Basing on in silico, in vitro and ex vivo models of translation processing, further studies revealed that eIF4E plays a critical role in the inhibitory effect of Ouabain on HIF-1α protein synthesis, rather than mTORC1, eIF2α signaling or Na + /K + -ATPase inhibition. (elsevier.com)
  • Ouabain-sensitive Na + -K + -stimulated ATPase was measured in red cell membranes using a spectrophotometric assay. (elsevier.com)
  • Na,K)-ATPase and Ouabain binding in reticulocytes from dogs with high K and low K erythrocytes and their changes during maturation. (hokudai.ac.jp)
  • v) Ouabain binding capacity, as well as (Na,K)-ATPase activity, in the reticulocytes from LK dogs fell rapidly to nearly zero during the maturation into erythrocytes. (hokudai.ac.jp)
  • vi) Although reticulocytes from HK dogs also showed a similar regression of (Na,K)-ATPase during maturation, they retained a certain number of ouabain binding sites even after maturation, resulting in the high activity of (Na,K)-ATPase in HK erythrocyte membrane. (hokudai.ac.jp)
  • Ouabain was used as a specific probe for inhibition of Na(+)-K+ ATPase and its known consequences on Na+ and Ca2+ translocation. (cornell.edu)
  • This pointed to Na+ loading of nerve terminals via high frequency stimulation plus ouabain inhibition of Na(+)-K+ ATPase. (cornell.edu)
  • Spectrophotometric method for the determination of renal ouabain-sensitive H+,K+ -ATPase activity. (edu.pl)
  • Both ouabain-sensitive and ouabain-resistant K+-stimulated and Na+-independent ATPase activity was detected in the renal cortex and medulla. (edu.pl)
  • The method for ouabain- sensitive H+,K+-ATPase assay is characterized by good reproducibility, linearity and recovery. (edu.pl)
  • In contrast, the assay for ouabain-resistant H+,K+-ATPase was unsatisfactory, probably due to low activity of this enzyme. (edu.pl)
  • Ouabain-sensitive H+,K+-ATPase was stimulated by K+ with Km of 0.26 ± 0.04 mM and 0.69 ± 0.11 mM in cortex and medulla, respectively, and was inhibited by ouabain (Ki of 2.9 ± 0.3 μM in the renal cortex and 1.9 ± 0.4 μM in the renal medulla) and by Sch 28080 (Ki of 1.8 ± 0.5 μM and 2.5 ± 0.9 μM in cortex and medulla, respectively). (edu.pl)
  • We found that ouabain-sensitive H+,K+-ATPase accounted for about 12% of total ouabain-sensitive activity in the Na+,K+-ATPase assay. (edu.pl)
  • Leptin administered intraperitoneally (1 mg/kg) decreased renal medullary Na+,K+-ATPase activity by 32.1% at 1 h after injection but had no effect on H+,K+-ATPase activity suggesting that the two renal ouabain-sensitive ATPases are separately regulated. (edu.pl)
  • 2000) Ouabain-insensitive Na+-ATPase activity is an effector protein for cAMP regulation in basolateral membranes of the proximal tubule. (edu.pl)
  • The effects of the opioid-type stressor, immobilization, on severity of ouabain-induced cardiac arrhythmias and the possible involvement of serum catecholamines were investigated in rats. (biomedsearch.com)
  • Thus, we prepared cochlear organotypic cultures from postnatal day-3 rats and treated these cultures with ouabain at 50, 500, and 1000 μ M for different time to elucidate the ototoxic effects of ouabain in vitro and to provide insights that could explain the comparative ototoxic effects of ouabain in vivo . (hindawi.com)
  • Erttmann RR, Damm KH (1974) Probenecid and hepatic transport of 3 H-ouabain in rats. (springer.com)
  • Proof of principle that ouabain rescues development of embryonic kidneys exposed to malnutrition was obtained from studies on pregnant rats given a low-protein diet and treated with ouabain or vehicle throughout pregnancy. (pubmedcentralcanada.ca)
  • To examine whether ouabain-calcium-NF-κB signalling might rescue the development of fetal kidneys exposed to malnutrition, we first used kidney explants from 14-day-old embryonic rats held in culture for 3 days. (pubmedcentralcanada.ca)
  • In the current study, on conscious rats, we examined (1) whether intrathecally administered ouabain produces antinociceptive effects, and (2) whether it modulates the antinociceptive actions of spinally administered morphine and lidocaine on somatic nociception. (asahq.org)
  • Importantly, the researchers found that their modified form ouabain produced no toxic effects in the rats. (medicalnewstoday.com)
  • Forty male Sprague-Dawley (SD) rats weighting 180-220 g were equally divided into normal control group and ouabain group randomly. (bvsalud.org)
  • Rats were infused with normal saline 1 ml/ (kg x d) or ouabain 27.8 microg/ (kg-d) intraperitoneally once a day respectively. (bvsalud.org)
  • The reabsorption of sodium increases in the proximal tubule in ouabain -hypertensive rats , and such increase occurs before the development of hypertension . (bvsalud.org)
  • However, the magnitude of this increase was larger for the hypertensive 1K1C and DOCA-salt rats than for normotensive animals, while the pressor effect of 0.18 µg/kg ouabain was greater only in DOCA-salt rats. (ufes.br)
  • In all animals, 10 nM ouabain increased the PHE pressor response, but this increase was larger in hypertensive DOCA-salt rats than in normotensive and 1K1C rats. (ufes.br)
  • c) hypertensive and uninephrectomized rats were less sensitive to toxic doses of ouabain, and d) after treatment with 10 nM ouabain isolated tail vascular beds from DOCA-salt rats were more sensitive to the pressor effect of PHE than those from normotensive and 1K1C hypertensive rats. (ufes.br)
  • The exogenous digitalis glycosides, ouabain and digoxin, have been widely used in humans to treat congestive heart failure and cardiac arrhythmias. (jimmunol.org)
  • These three mAbs are distinguished from existing Abs to ouabain and digoxin by their specificity for ouabain and lack of cross-reactivity with digoxin. (jimmunol.org)
  • Recent studies demonstrate the presence of a digoxin analog, ouabain, in mammalian tissue, but its biological role has not been elucidated. (diva-portal.org)
  • Impact of neonatal treatment with cardioactive glycosides (digoxin, ouabain) on receptor binding capacity, blood level and cardiac function in the adult rat. (elsevier.com)
  • A single neonatal treatment with a cardioactive glycoside (ouabain, digoxin) altered the response of the adult rat to digitaloid treatment. (elsevier.com)
  • The pump inhibitor ouabain (1 mmol l -1 ) depolarized V bl , but addition of furosemide to bath solution did not affect V bl . (biologists.org)
  • An increase 9 as well as a decrease of plasma concentrations of ouabain-like compounds (OLCs) 10,11 have been reported. (ahajournals.org)
  • As expected, serum-deprived kidneys displayed severe retardation of nephron formation and robust increase in apoptotic index (AI), but these effects were abolished if the kidneys were exposed to ouabain in concentrations that had no effect on intracellular sodium. (pubmedcentralcanada.ca)
  • Ouabain, a representative cardenolide, was found to potently diminish TGEV titers and inhibit the TGEV-induced production of IL-6 in a dose dependent manner, with 50% inhibitory concentrations of 37 nM and 23 nM respectively. (bioportfolio.com)
  • We assessed the sensitivity of two techniques for measuring inhibition of cellular (Na+/K+ pumps by ouabain at low concentrations in vitro. (nus.edu.sg)
  • We incubated lymphocytes from healthy subjects for 1 h and 72 h with unlabelled ouabain at different concentrations to determine the lowest concentration which inhibits specific 3H-ouabain binding (the number of Na+/K+ pumps) and ouabain sensitive 86rubidium influx (pump activity). (nus.edu.sg)
  • The lowest ouabain concentrations which significantly reduced Na+/K+ pump numbers and activity during 72 h incubation were 5 x 10-9 and 1 x 10-8 mol L-1, which approximate the sensitivity of radioimmunoassay for plasma ouabain. (nus.edu.sg)
  • Preincubation of bone marrow cells with ouabain at concentrations of 10 -6 -10 -12 M suppressed the proliferation of CFU-S as measured by the spleen colony assay. (elsevier.com)
  • Rat cardiac myocytes treated with 50 μM ouabain demonstrated substantial increases in systolic and diastolic Ca 2+ concentrations. (marshall.edu)
  • Ouabain binding by the human erythrosyte membrane is reversible, exhibits a high of chemical specificity, and can be detected at ouabain concentrations as low as lxlO-l0M. (kzoo.edu)
  • Results showed that independent of axon size, both ouabain and anoxia markedly increased axoplasmic Na and decreased K concentrations. (elsevier.com)
  • Once ouabain binds to this enzyme, the enzyme ceases to function, leading to an increase of intracellular sodium. (wikipedia.org)
  • This suggests that ouabain probably binds to a protein. (kzoo.edu)
  • Mechanistically, Ouabain directly binds eIF4E, disrupts eIF4E/eIF4G association (200 μM, Inhibit rate = 61 ± 3%) but not the eIF4E/mRNA complex formation (200 μM, Inhibit rate = 18 ± 5%) both in vitro and in cells, thereby inhibiting the intracellular cap-dependent translation. (elsevier.com)
  • The results demonstrate a new mechanism for steroid signaling via plasma membrane receptors and underline a novel role for the steroid hormone, ouabain, as a physiological inducer of [Ca2+](i) oscillations involved in transcriptional regulation in mammalian cells. (diva-portal.org)
  • Ouabain, that has been isolated from bovine adrenals and hypothalamus, is a new cardiotonic steroid hormone, which is either synthesized in the adrenals or stored there after it has absorbed from the diet. (nii.ac.jp)
  • The cardiotonic steroid binding globulin shows the highest concentration in the kidney, which suggests that sodium pumps of the kidney are protected against its inhibition by ouabain which would lead not only to natriuresis but also to a deleterious loss of glucose, amino acids and phosphate. (nii.ac.jp)
  • The structure provides key details on CTS binding, revealing an extensive hydrogen bonding network formed by the β-surface of the steroid core of ouabain and the side chains of αM1, αM2, and αM6. (forskningsdatabasen.dk)
  • There is growing evidence that ouabain, a cardiotonic steroid may promote growth of cardiac and vascular myocytes, indicating its novel role in cell growth and proliferation, without appreciable inhibition of the sodium pump. (bvsalud.org)
  • We investigated uptake of ouabain into isolated rat hepatocytes and release of ouabain from preloaded hepatocytes, thus assessing separately the two membrane transport steps, involved in biliary elimination of the drug. (springer.com)
  • Release of ouabain from preloaded cells was energyindependent, independent of the Na + -concentration and not susceptible to inhibition by dinitrofluorobenzene or taurocholate. (springer.com)
  • which events may lead to the release of ouabain into blood. (nii.ac.jp)
  • Schwann cells and myelin displayed small increases in Na and substantial losses of K in response to ouabain exposure. (elsevier.com)
  • In assays of fluorescence quenching and saturation equilibrium with tritiated ouabain, Ab 1-10 exhibited 200 nM affinity for ouabain. (jimmunol.org)
  • Either the number of ouabain binding sites and/or the apparent affinity for ouabain were reduced for those with DLF levels in all three groups. (elsevier.com)
  • The safety and delivery efficiency of ouabain in vivo was also improved, suggesting a therapeutic application of ouabain double emulsion could be benefit in canine osteosarcoma targeting therapy. (aacrjournals.org)
  • 6. A single application of ouabain (10 microM) to CA3 by local pressure ejection caused a slow rise in local [K+]o measured with K-ISMs. (nih.gov)
  • Ouabain-like compound changes rapidly on physical exercise in humans and dogs: effects of β -blockade and angiotensin-converting enzyme inhibition," Hypertension , vol. 45, no. 5, pp. 1024-1028, 2005. (hindawi.com)
  • The ouabain link to hypertension, reported in the Proceedings of the National Academy of Sciences by scientists at the University of Maryland, was found in a study of people who had their thyroid glands removed. (chicagotribune.com)
  • In related work, Baltimore scientists collaborating with researchers from the UpJohn Co. in Kalamazoo, Mich., found that a substance that regulates sodium levels in cells also turns out to be ouabain, which may explain the link between salt consumption and hypertension in some people. (chicagotribune.com)
  • Therefore, increase of proximal reabsorption of sodium may be involved in the pathogenesis of ouabain -induced hypertension . (bvsalud.org)
  • Rostafuroxin (PST-2238) is an ouabain-inhibitor counteracting specific forms of hypertension. (adooq.com)
  • Ouabain increases vascular resistance and may induce hypertension by inhibiting the Na+ pump. (ufes.br)
  • Ouabain is eliminated by renal excretion, largely unchanged. (wikipedia.org)
  • 1. The influence of single-dose intravenous ouabain on haemodynamics, renal function and vasoactive hormones was investigated in healthy subjects. (clinsci.org)
  • Sodium-dependence and ouabain-sensitivity of the synthesis of dopamine in renal tissues of the rat. (mysciencework.com)
  • Ouabain and Insulin Induce Sodium Pump endocytosis in Renal Epithelium" by Shalini Gupta, Yanling Yan et al. (marshall.edu)
  • In 1991, a specific high affinity sodium pump inhibitor indistinguishable from ouabain was first discovered in the human circulation and proposed as one of the potential mediators of long term blood pressure and the enhanced salt excretion following salt and volume loading. (wikipedia.org)
  • Yet, most of the mutagenesis data obtained with the high-affinity state are readily explained by the present crystal structure, indicating that the binding site for ouabain is essentially the same. (pnas.org)
  • Most importantly, the A domain has rotated in response to phosphorylation and αM1-2 move towards the ouabain molecule, providing for high affinity interactions and closing the ion pathway from the extracellular side. (rcsb.org)
  • In both types of cells [3H]-ouabain binding was saturable, confined to a single site of high affinity, slow to reach equilibrium, slow to reverse, temperature-dependent, competitively antagonized by potassium, and facilitated by the presence of divalent cations. (ox.ac.uk)
  • The predominant fraction of intracellular ouabain beeing unbound. (springer.com)
  • To assess the role of the ouabain-calcium-NF-κB signalling pathway, we deprived the intracellular stores of calcium and blocked the transcriptional capacity of NF-κB. (pubmedcentralcanada.ca)
  • Intracellular sodium affects ouabain interaction with the Na/K pump in cultured chick cardiac myocytes. (rupress.org)
  • OUABAIN is a specific inhibitor of membrane-bound Na + ,K +-adenosine triphosphatase (Na +-K + pump), which regulates the intracellular Na + ([Na + ] in ) and K + ([K + ] in ) content of a variety of cell types, including in the central nervous system. (asahq.org)
  • Exposure to 10 μM H 2 O 2 , which causes an increase in intracellular reactive oxygen species similar to that of 50 μM ouabain, caused a similar increase in τ. (marshall.edu)
  • It was already shown that ouabain treatment can stimulate PKC isoenzymes leading to the activation of intracellular pathways involved in cell survival , growth and proliferation. (bvsalud.org)
  • Ouabain increased dP/dt similarly by 600 ± 90 mm Hg/sec, but did not change heart rate or mean arterial pressure significantly. (ovid.com)
  • Incubation of cells with ouabain for 1 h did not significantly affect Na+/K+ pump activity. (nus.edu.sg)
  • The recovery time constant for the Ca 2+ transient, τ, was significantly prolonged by ouabain. (marshall.edu)
  • Phenylephrine and yohimbine potentiated the cardiotoxicity of ouabain significantly whereas prazosin and clonidine showed significant antiarrhythmic effects and delayed the cardiac arrest. (meta.org)
  • Ouabain induces Rho-dependent rock activ. (gazi.edu.tr)
  • As it was shown by us previously, ouabain induces membrane blebbing and proteolitic cleavage of ROCK I and ROCK II via caspases in human umbilical endothelial cells. (gazi.edu.tr)
  • Ouabain induces concentration-dependent cell death and membrane blebbing in endothelial cells. (gazi.edu.tr)
  • We propose that if ouabain is present in the human circulation, its concentration is less than 0.005 nmol/L. (ahajournals.org)
  • We assume, that the high bile/plasma concentration-gradient of ouabain in vivo is generated during active uptake into the cell and not during release into bile. (springer.com)
  • Ouabain is a cardiac glycoside that inhibits the enzyme responsible for regulating the concentration of sodium and potassium ions within cardiac muscle cells. (blogspot.com)
  • Thus, ouabain was shown not to undergo metabolism disorder in the small intestine over the low auxin concentration range at which porfimer sodium oleate was shown to undergo extensive oxidative metabolism. (good4youherbals.com)
  • The values of maximal specific ouabain binding, measured by Scatchard analysis of concentration vs binding curves (Bmax), were 33.9 +/- 6.0 fmol/10(6) cells (polymorphs) and 59.3 +/- 11.6 fmol/10(6) cells (mononuclear cells) (P less than 0.02). (ox.ac.uk)
  • In comparison with un JNK-IN-8 concentration treated cells we observed a pronounced increase (100±20%) of CDCF fluorescence when Milciclib U937 cells were treated with ouabain 1 μM and no increase when the concentration of ouabain was ≤500 nM (Figure 2a). (cox2-inhibitors.com)
  • The relation between ouabain binding and ouabain concentration can be described by a rectangular hyperbola permitting determination of the maximal, binding (Bmax) and the ouabain concentration at which ouabain binding is half-maximal (KB). (kzoo.edu)
  • Although 1 μM ouabain did not alter cytoplasmic calcium concentration, it stimulated calcium entry after store depletion, a response abolished by 2-APB. (elsevier.com)
  • We assessed the role of extracellular potassium ([K+]e) on the increase in cerebral blood flow (CBF) during hypoxia, and we tested whether it was affected by glibenclamide or ouabain. (ox.ac.uk)
  • The change in ionic gradients caused by ouabain can also affect the membrane voltage of the cell and result in cardiac arrhythmias. (wikipedia.org)
  • Ouabain, a specific inhibitor of active cation transport across cell membrane, was applied topically to brain surface of cats. (springer.com)
  • In prior studies, locally administering ouabain via round window membrane demonstrated that the ototoxic effects of ouabain in vivo varied among mammalian species. (hindawi.com)
  • In an attempt to mitigate such narrow therapeutic margin of safety, ouabain was formulated in lipid double emulsion by using microfluidic synthesis method or membrane emulsification method. (aacrjournals.org)
  • To show that the [3H]ouabain had free access to all of the membrane surfaces within the epithelium, in parallel experiments we incubated isolated processes in horseradish peroxidase. (arvojournals.org)
  • Therefore, the aim of the present work was the comparative study of the modulation effect of ELF-EMF on cisplatin-induced changes cell hydration and number of ouabain receptors in membrane of sarcoma-180 tumor tissues. (springer.com)
  • The number of 3 H-ouabain receptors in membrane was counted by isotope scintillation counter. (springer.com)
  • The aim of this study was to identify the possible role of Rho in ouabain-induced membrane blebbing. (gazi.edu.tr)
  • Pretreatment of endothelial cells with a Rho inhibitor CT04 did not inhibit the ouabain-induced cell death but prevented the development of bleb formation. (gazi.edu.tr)
  • Ouabain pretreatment doubled the duration over which single stimuli, following either high frequency or edrophonium conditioning produced PTR. (cornell.edu)
  • Ouabain, an inhibitor of the sodium pump, has been identified as a constituent of bovine adrenal glands. (ahajournals.org)
  • Kupferberg HJ, Schanker LS (1968) Biliary secretion of ouabain- 3 H and its uptake by liver slices in the rat. (springer.com)
  • The number of colonies formed by BFU-E and CFU-E in plasma clot cultures was also diminished in the presence of 10 -4 M ouabain. (elsevier.com)
  • Ouabain is a common tool to explore the pathophysiological changes in adult mammalian cochlea in vivo . (hindawi.com)
  • These Abs can be used to develop assays for detection and characterization of ouabain-like molecules in vivo. (jimmunol.org)
  • Our preliminary results showed enhanced antitumor activities of ouabain in double emulsion or nanoemulsion against canine osteosarcoma cell lines in vitro and in orthotopic xenograft models in vivo. (aacrjournals.org)
  • Administration of ouabain in vivo or in vitro leads to corneal swelling and a marked reduction in the number of pinocytotic vesicles in the endothelium. (arvojournals.org)
  • The interactions of ouabain with post-tetanic and facilitatory drug potentiations at cat soleus neuromuscular junctions in vivo. (cornell.edu)
  • Previous data from our group described that ouabain treatment increases retinal ganglion cells survival (RGC). (springer.com)
  • We previously showed that ouabain increases proliferation of cultured human ADPKD cells via stimulation of the EGF receptor (EGFR)-Src-MEK/ERK signaling pathway. (semanticscholar.org)
  • We also observed that 50 μM ouabain induced increases in developed pressure in addition to diastolic dysfunction in the isolated perfused rat heart. (marshall.edu)
  • Coadministration of ouabain with N -acetylcysteine prevented these increases. (marshall.edu)
  • It is concluded that selective alpha 1-receptor stimulation and alpha 2-receptor blockade increases the cardiotoxic effects of ouabain and selective alpha 2-receptor stimulation and alpha 1-receptor blockade inhibits ouabain-induced arrhythmias and cardiac arrest in guinea-pigs. (meta.org)
  • We have previously demonstrated that ouabain or PMA treatment increases retinal ganglion cell survival , an effect mediated by PKC activation. (bvsalud.org)
  • 0.05) and ouabain (100 and 500 microM) was no longer able to reduce the accumulation of both newly-formed dopamine and DOPAC.4. (mysciencework.com)
  • ouabain (100, 500 and 1000 microM) was found to affect neither the formation of dopamine nor its deamination to DOPAC when homogenates were used instead of kidney slices.5. (mysciencework.com)
  • Lanthanum (1 mM) and cobalt (2 mM), two inorganic Ca++-entry blockers, were able to inhibit this stimulatory effect, whereas verapamil (10 microM) and nitrendipine (50 microM), two organic antagonists of the voltage-operated channel for Ca++ ions, failed to affect ouabain-induced (3H)DA release. (osti.gov)
  • By contrast, adriamycin (100-300 microM), a putative inhibitor of cardiac Na+-Ca++ antiporter, dose-dependently prevented ouabain-induced (3H)DA release from TIDA neurons. (osti.gov)
  • Our results show that 2.5 microM tyrphostin, 1.0 microM PP1, 4.0 microM U73122, 1.0 microM JNK inhibitor V and 2.0 microM rottlerin blocked the ouabain effect indicating an involvement of receptors for EGF , Src, PLC, JNK and PKC delta respectively. (bvsalud.org)
  • The major apoptotic pathways in ouabain-induced spiral ganglion neuron apoptosis culminated in the stimulation of the p53 pathway and triggering of apoptosis by a network of proapoptotic signaling pathways. (hindawi.com)
  • Similarly to ouabain, OLC enhanced the release of (3H)NA in response to electrical stimulation. (elsevier.com)
  • There was stimulation with both magnesium and ATP or with magnesium alone, but stimulation of ouabain was three times greater in the presence of ATP. (kzoo.edu)
  • Other monovalent cations inhibited ouabain binding in the presence of ATP and showed little stimulation in the absence of ATP. (kzoo.edu)
  • Treatment with ouabain resulted in SGLT1 inhibition at 1 h but stimulation at 24 h. (nih.gov)
  • Here we report stimulation of sodium-hydrogen exchange (NHE) in ouabain-treated cells. (elsevier.com)
  • Ouabain-induced stimulation of pH recovery was suppressed by inhibitors of capacitative calcium entry, SKF-96365, and 2-APB, as well as the cytoplasmic calcium chelator BAPTA. (elsevier.com)
  • Taken together, the results are consistent with increased capacitative calcium entry and subsequent cAMP-PKA-dependent stimulation of NHE1 in ouabain-treated cells. (elsevier.com)
  • Mandal, A , Delamere, NA & Shahidullah, M 2008, ' Ouabain-induced stimulation of sodium-hydrogen exchange in rat optic nerve astrocytes ', American Journal of Physiology - Cell Physiology , vol. 295, no. 1, pp. (elsevier.com)
  • The effects of stimulation frequency, ouabain and the sequence of events leading to PTR in the soleus neuromuscular system appeared in general no different from those derived from the many in vitro microphysiologic studies of this phenomenon. (cornell.edu)
  • The results showed that ouabain-induced damage in vitro was dose and time dependent. (hindawi.com)
  • In the present investigation, electron probe X-ray microanalysis was used to characterize the effects of in vitro ouabain (2 mM) or anoxia on elemental composition (e.g. (elsevier.com)
  • 7 Bovine adrenocortical cells in tissue culture release ouabain on exposure to epinephrine, angiotensin II, or corticotropin. (ahajournals.org)
  • In conclusion, equi-inotropic doses of ouabain and isoproterenol induced opposite effects on the mechanical function, ECGs and blood flow of severely ischemic tissue. (ovid.com)
  • No label was found in sections of control tissue which had been incubated in [3H]ouabain with an excess of cold ouabain. (arvojournals.org)
  • Danielyan AA, Mirakyan MM, Grigoryan GY, Ayrapetian SN (1999) The static magnetic field on ouabain H3 binding by cancer tissue. (springer.com)
  • Methods: We used conventional microelectrodes to record the action potentials (APs) in isolated young (age, 3 months) and aged (age, 3 years) rabbit LA posterior wall (LAPW) and LA appendage (LAA) tissue specimens before and after the administration of ouabain. (elsevier.com)
  • Immunomodulatory activity of ouabain in Leishmania leishmania amazonensis-infected Swiss mice," Parasitology Research , vol. 112, pp. 1313-1321, 2013. (hindawi.com)
  • Finally, inhibition of PI3K by LY294002 or PDK1 by BX795 antagonized the anti-viral activity of ouabain and restored the TGEV virus titer and yields. (bioportfolio.com)
  • However, when ouabain was coupled to an Ab of A/J mice origin and the same strain of mouse was used for immunization with ouabain-Ab conjugate, three Abs (1-10, 5A12, and 7-1) specific for ouabain were obtained. (jimmunol.org)
  • Characteristically, the responses to ouabain could be described by an initial rapid phase (5-10 min) during which time the Ri was increased markedly and the E'1 was decreased from control values. (rupress.org)
  • We conclude that specific 3H-ouabain binding and ouabain sensitive 86rubidium influx are similarly sensitive at detecting lymphocyte Na+/K+ pump inhibition by ouabain. (nus.edu.sg)
  • Before administration of ouabain, exercise was associated with depressed pumping performance of the left ventricle in four patients: stroke work fell with exercise while left ventricular end-diastolic pressure (LVEDP) increased. (ahajournals.org)
  • Administration of ouabain resulted in a decrease in aqueous flow and a drop in intraocular pressure, presumably due to an inhibition of sodium transport across the ciliary epithelium. (jamanetwork.com)
  • We used selective inhibitors of [Ca2+]i (BAPTA-AM), m-calpain (MDL28170), PKCα (Go6976) and determined their involvement in ouabain (10 nM)-mediated smooth muscle cell proliferation. (bvsalud.org)
  • However, caspase inhibitors do not prevent ouabain-induced blebs. (gazi.edu.tr)
  • Downstream effects of ouabain signalling include activation of the calcium-dependent transcription factor nuclear factor (NF)-κB and protection from apoptosis 16 . (pubmedcentralcanada.ca)
  • Considering that Ouabain (OUA) may prevent the apoptosis process, in this study we evaluated if OUA is able to avoid the damage caused by Stx2 and SubAB on human glomerular endothelial cells (HGEC) and the human proximal tubule epithelial cell (HK-2) line. (mdpi.com)
  • Norepinephrine after ouabain only decreased contractile force, dT/dt, ISF, time to peak force and duration of the cardiac cycle. (aspetjournals.org)
  • Corrêa GR, Cunha KCS, Santos AA, Araujo EG (2010) The trophic effect of ouabain on retinal ganglion cell is mediated by EGF receptor and PKC delta activation. (springer.com)
  • Either ouabain or insulin alone caused accumulation of and carcinoembryonic antigen cell adhesion molecule, as well as insulin receptor-β, and epidermal growth factor receptor in early endosomes, but no synergy was demonstrable. (marshall.edu)
  • Ouabain is a highly toxic compound with a LD50 of 5 mg/kg when administered orally to rodents. (wikipedia.org)
  • Identification and characterization of a ouabain-like compound from human plasma," Proceedings of the National Academy of Sciences of the United States of America , vol. 88, pp. 6259-6263, 1991. (hindawi.com)
  • Ouabain-like compound (OLC) was measured in venous blood by enzyme-linked immunosorbent assay as well as by 86 Rb + uptake inhibition (as ouabain equivalents). (ahajournals.org)
  • The latest study focuses on one compound in particular: ouabain. (medicalnewstoday.com)
  • Ouabain , a substance that derives from the African plants Strophanthus gratus and Acokanthera schimperi , is a toxic compound that, in high doses, can cause cardiac arrest. (medicalnewstoday.com)
  • Effects of alpha-adrenoceptor agonists and antagonists with different affinity for alpha 1- and alpha 2-receptors on ouabain-induced arrhythmias in guinea-pigs were studied. (meta.org)
  • The aim of this work was to investigate the role of EGF receptors in the ouabain effect and also to study which PKC isoform is activated by treatment with ouabain and PMA. (bvsalud.org)
  • Treatment with 18 µg/kg ouabain increased systolic and diastolic blood pressure in all groups studied. (ufes.br)
  • We also demonstrated a synergistic effect on RGC survival when we concomitantly used ouabain (0.75 nM) and BDNF (10 ng/mL). (springer.com)
  • Because local anesthetic agents block the generation of neural action potentials and their propagation by a selective effect on Na + channels of neuronal membranes and K + channels as well, [7] especially blocking Na + influx through Na + - selective pores, there could be a significant interaction between the effects of ouabain and those of local anesthetic agents. (asahq.org)
  • Clonal assays for CFU-S, CFU-C, BFU-E, and CFU-E were employed to evaluate the effect of ouabain on the proliferation of mouse hematopoietic progenitor cells. (elsevier.com)
  • Effect of m-calpain in PKCα-mediated proliferation of pulmonary artery smooth muscle cells by low dose of ouabain. (bvsalud.org)
  • This inhibitory effect was sustained when cells were treated with ouabain for 24 h. (nih.gov)
  • effect of ouabain on phlorizin-sensitive 3- O -methyl- d -[ 3 H]glucose ([ 3 H]OMG) uptake in IEC-18 cells. (nih.gov)
  • In bicarbonate-free medium, dimethylamiloride, an NHE inhibitor, eliminated the effect of 1 μM ouabain on pH recovery. (elsevier.com)
  • Ouabain evoked an increase of cAMP, and the effect of 1 μM ouabain on pH recovery was abolished by H-89, a protein kinase A inhibitor. (elsevier.com)
  • It was the purpose of this investigation to study the effect of ouabain on the active transport of sodium across the corneal epithelium. (jamanetwork.com)
  • Role of K+ in regulating hypoxic cerebral blood flow in the rat: effect of glibenclamide and ouabain. (ox.ac.uk)
  • Ouabain in the doses used had no effect per se but as a function of dose augmented the frequency dependent responses. (cornell.edu)
  • Ouabain promotes partial epithelial to mesenchymal transition (EMT) changes in human autosomal dominant polycystic kidney disease (ADPKD) cells. (semanticscholar.org)
  • The response of the left ventricle to a level of supine bicycle exercise that induced angina, and the effects of ouabain (0.01 mg/kg) on this response were evaluated in 14 patients with coronary arterial disease. (ahajournals.org)
  • Ouabain as a mammalian hormone," Annals of the New York Academy of Sciences , vol. 986, pp. 678-684, 2003. (hindawi.com)
  • Meijer DK, Vonk RJ, Scholtens EJ, Levine WG (1976) The influence of dehydrocholate on hepatic uptake and biliary excretion of 3 H-taurocholate and 3 H-ouabain. (springer.com)
  • Russell JQ, Klaassen CD (1972) Species variations in the biliary excretion of ouabain. (springer.com)
  • Ouabain-induced spontaneous activity was similar between the young and aged groups. (elsevier.com)
  • Addition of ouabain (10/sup -4/ M) during the contractile phase of spontaneous activity, increased the frequency of oscillations which appeared to fuse into a tetanus. (osti.gov)
  • 500 μ M ouabain and 1000 μ M ouabain were destructively traumatic to both spiral ganglion neurons and cochlear hair cells in an apoptotic signal-dependent pathway. (hindawi.com)
  • Ouabain activates NF κ B through an NMDA signaling pathway in cultured cerebellar cells," Neuropharmacology , vol. 73, pp. 327-336, 2013. (hindawi.com)
  • Ouabain-insensitive salt and water movements in duck red cells. (rupress.org)
  • Incubation in ouabain led to a fall in the K+/Na+, K+/P, and K+/Cl- ratios and a rise in the Na+/P and Na+/Cl- ratios in both neoplastic and nonneoplastic cells and effectively nullified the difference between them. (lancs.ac.uk)
  • Studies were done with isolated frog skin to determine the effects of 10(-4) M ouabain on the electrophysiological parameters of outer and inner barriers of the Na-transporting cells. (rupress.org)
  • And researchers seeking to develop a male contraceptive pill have reported that molecules derived from ouabain can bind to a protein that is only found in sperm cells, and which interferes with sperms' ability to swim. (blogspot.com)
  • When included in soft agar cultures of bone marrow cells, ouabain suppressed the proliferation of CFU-C in a complex fashion. (elsevier.com)
  • Our results suggested that treatment of bovine pulmonary artery smooth muscle cells with a low dose of ouabain (10 nM) increased [Ca2+]i and subsequently stimulated m-calpain activity and proteolytically activated PKCα in caveolae (signaling microdomain also known as signalosomes) of the cells. (bvsalud.org)
  • Immunoprecipitation studies showed phosphorylation of NHE1 in cells treated with 1 μM ouabain. (elsevier.com)
  • The cAMP increase in ouabain-treated cells was abolished by BAPTA and 2-APB. (elsevier.com)
  • Lantz, RC , Elsas, LJ & DeHaan, RL 1980, ' Ouabain-resistant hyperpolarization induced by insulin in aggregates of embryonic heart cells ', Proceedings of the National Academy of Sciences of the United States of America , vol. 77, no. 5 I, pp. 3062-3066. (elsevier.com)
  • iv) Ouabain-sensitive fluxes of 24Na and 42K in each type of reticulocyte were compatible with the number of ouabain binding sites on the cells. (hokudai.ac.jp)
  • To obtain proof of principle that ouabain rescues the development of embryonic kidneys exposed to malnutrition, nephron endowment was examined in offspring of rat dams that had been given a low-protein diet and treated with ouabain or vehicle throughout pregnancy. (pubmedcentralcanada.ca)
  • Here, we analyzed the effects of low dose of ouabain (10 nM) on increase in [Ca2+]i, m-calpain and protein kinase C (PKC) activities on pulmonary artery smooth muscle cell proliferation and determined their sequential involvement in this scenario. (bvsalud.org)
  • In the consolidating trace, ouabain is thought to inhibit an intermediate-term memory phase, some aspects of which acts as a "trigger" for long-term stabilisation of the trace by new protein synthesis. (edu.au)
  • In the present study, we observed that neither HIF-1α mRNA levels nor HIF-1α protein degradation are affected by Ouabain. (elsevier.com)
  • An enzyme-linked immunosorbent assay is described for the measurement of ouabain in human plasma. (ahajournals.org)
  • We have examined the localization of this enzyme in the epithelium of rabbit ciliary body pars plicata using [3H]ouabain autoradiography. (arvojournals.org)
  • At 10 -4 M ouabain, colony formation was suppressed by 70%, while at 10 -6 M ouabain, CFU-C proliferation was normal. (elsevier.com)
  • at 10 -12 M ouabain, CFU-E proliferation was unaffected by the drug. (elsevier.com)
  • The mechanism(s) by which low dose of ouabain produces pulmonary artery smooth muscle cell proliferation, a prerequisite for right ventricular hypertrophy, is currently unknown. (bvsalud.org)
  • Thus, [Ca2+]i-mCalpain-PKCα signaling axis plays a crucial role during low dose of ouabain-mediated pulmonary artery smooth muscle cell proliferation. (bvsalud.org)
  • Rat tail vascular beds were perfused to analyze the effects of 10 nM ouabain on the pressor response to PHE. (ufes.br)
  • When high-performance liquid chromatographic profiles of plasma spiked with ouabain standard or following bolus intravenous injections of ouabain into normal human volunteers were compared with profiles of unspiked plasma, there was no support for the immunoreactive material in the latter samples being ouabain. (ahajournals.org)
  • Early arrhythmias, ventricular fibrillation and cardiac arrest were induced in anaesthetized guinea-pigs by the slow intravenous infusion of ouabain. (meta.org)
  • Both plasma atrial and brain natriuretic peptide levels were increased after ouabain administration. (clinsci.org)
  • The cerebral cortex to which 10 −3 M ouabain was applied showed a spongy state, which was ultrastructurally revealed to be swelling of the neuronal cell processes, especially of dendrites. (springer.com)
  • Uptake of ouabain was competitively inhibited by the cardiac glycoside digitoxin ( K i =0.3 μM). (springer.com)