A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Methods for maintaining or growing CELLS in vitro.
Techniques used in studying bacteria.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. The tissue is cultured directly after removal from the host without being dispersed for cell culture.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.
Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Techniques used in microbiology.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Methods for cultivation of cells, usually on a large-scale, in a closed system for the purpose of producing cells or cellular products to harvest.
Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.
The vein which drains the foot and leg.
Either of the two principal arteries on both sides of the neck that supply blood to the head and neck; each divides into two branches, the internal carotid artery and the external carotid artery.
The vessels carrying blood away from the capillary beds.
A class of enzymes that catalyzes the degradation of gelatin by acting on the peptide bonds. EC 3.4.24.-.
A family of zinc-dependent metalloendopeptidases that is involved in the degradation of EXTRACELLULAR MATRIX components.
A secreted endopeptidase homologous with INTERSTITIAL COLLAGENASE, but which possesses an additional fibronectin-like domain.
Enlarged and tortuous VEINS.
Axons of certain cells in the DENTATE GYRUS. They project to the polymorphic layer of the dentate gyrus and to the proximal dendrites of PYRAMIDAL CELLS of the HIPPOCAMPUS. These mossy fibers should not be confused with mossy fibers that are cerebellar afferents (see NERVE FIBERS).
GRAY MATTER situated above the GYRUS HIPPOCAMPI. It is composed of three layers. The molecular layer is continuous with the HIPPOCAMPUS in the hippocampal fissure. The granular layer consists of closely arranged spherical or oval neurons, called GRANULE CELLS, whose AXONS pass through the polymorphic layer ending on the DENDRITES of PYRAMIDAL CELLS in the hippocampus.
Specialized junctions at which a neuron communicates with a target cell. At classical synapses, a neuron's presynaptic terminal releases a chemical transmitter stored in synaptic vesicles which diffuses across a narrow synaptic cleft and activates receptors on the postsynaptic membrane of the target cell. The target may be a dendrite, cell body, or axon of another neuron, or a specialized region of a muscle or secretory cell. Neurons may also communicate via direct electrical coupling with ELECTRICAL SYNAPSES. Several other non-synaptic chemical or electric signal transmitting processes occur via extracellular mediated interactions.
The capacity of the NERVOUS SYSTEM to change its reactivity as the result of successive activations.
Depolarization of membrane potentials at the SYNAPTIC MEMBRANES of target neurons during neurotransmission. Excitatory postsynaptic potentials can singly or in summation reach the trigger threshold for ACTION POTENTIALS.
The communication from a NEURON to a target (neuron, muscle, or secretory cell) across a SYNAPSE. In chemical synaptic transmission, the presynaptic neuron releases a NEUROTRANSMITTER that diffuses across the synaptic cleft and binds to specific synaptic receptors, activating them. The activated receptors modulate specific ion channels and/or second-messenger systems in the postsynaptic cell. In electrical synaptic transmission, electrical signals are communicated as an ionic current flow across ELECTRICAL SYNAPSES.
A persistent increase in synaptic efficacy, usually induced by appropriate activation of the same synapses. The phenomenological properties of long-term potentiation suggest that it may be a cellular mechanism of learning and memory.
Congenital structural abnormalities of the UROGENITAL SYSTEM in either the male or the female.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
Genes that inhibit expression of the tumorigenic phenotype. They are normally involved in holding cellular growth in check. When tumor suppressor genes are inactivated or lost, a barrier to normal proliferation is removed and unregulated growth is possible.
Isoforms encoded by the WT1 Wilms tumor suppressor gene (GENES, WILMS TUMOR) and produced by alternative splicings. They are zinc finger-containing transcription factors involved in both transactivation and repression, and are critical for normal development and function of the urogenital tract.
All the organs involved in reproduction and the formation and release of URINE. It includes the kidneys, ureters, BLADDER; URETHRA, and the organs of reproduction - ovaries, UTERUS; FALLOPIAN TUBES; VAGINA; and CLITORIS in women and the testes; SEMINAL VESICLES; PROSTATE; seminal ducts; and PENIS in men.
A paired box transcription factor that is essential for ORGANOGENESIS of the CENTRAL NERVOUS SYSTEM and KIDNEY.
Genes at loci that are involved in the development of WILMS TUMOR. Included are human WT1 at 11p13 and human WT2 (MTACR1) at 11p15.
Occupations of medical personnel who are not physicians, and are qualified by special training and, frequently, by licensure to work in supporting roles in the health care field. These occupations include, but are not limited to, medical technology, physical therapy, physician assistant, etc.
Books used in the study of a subject that contain a systematic presentation of the principles and vocabulary of a subject.
Development of a library collection, including the determination and coordination of selection policy, assessment of needs of users and potential users, collection use studies, collection evaluation, identification of collection needs, selection of materials, planning for resource sharing, collection maintenance and weeding, and budgeting.
The biological science concerned with the life-supporting properties, functions, and processes of living organisms or their parts.
One of the BIOLOGICAL SCIENCE DISCIPLINES concerned with the origin, structure, development, growth, function, genetics, and reproduction of animals, plants, and microorganisms.
A disorder characterized by aching or burning sensations in the lower and rarely the upper extremities that occur prior to sleep or may awaken the patient from sleep.
Green dyes containing ammonium and aryl sulfonate moieties that facilitate the visualization of tissues, if given intravenously. They have mostly been used in the study of kidney physiology.
Corneal and conjunctival dryness due to deficient tear production, predominantly in menopausal and post-menopausal women. Filamentary keratitis or erosion of the conjunctival and corneal epithelium may be caused by these disorders. Sensation of the presence of a foreign body in the eye and burning of the eyes may occur.
A plant family of the order Eucommiales, subclass Hamamelidae, class Magnoliopsida (some botanists have classified this in the order Hamamelidales or Urticales). Eucomia is an elmlike tree of central and eastern China. Leaves are alternate; deciduous flowers are solitary and unisexual and lack petals and sepals. The male flowers have 6 to 10 stamens and female flowers have one ovary of two carpels, one of which aborts during development so the fruit (a dry, winged structure) contains only one seed. The latex is a source of RUBBER. Tochu tea is an aqueous extract of Eucommia ulmoides leaves and a popular beverage in Japan. (Mutat Res 1997 Jan 15;388(1):7-20).
Rare cutaneous eruption characterized by extensive KERATINOCYTE apoptosis resulting in skin detachment with mucosal involvement. It is often provoked by the use of drugs (e.g., antibiotics and anticonvulsants) or associated with PNEUMONIA, MYCOPLASMA. It is considered a continuum of Toxic Epidermal Necrolysis.
A plant genus of the family AQUIFOLIACEAE. The common name of 'holly' usually refers to this genus but may sometimes refer to similar looking plants of the MAHONIA or QUERCUS genus.
Fibrous bands or cords of CONNECTIVE TISSUE at the ends of SKELETAL MUSCLE FIBERS that serve to attach the MUSCLES to bones and other structures.
Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. The use of TISSUE SCAFFOLDING enables the generation of complex multi-layered tissues and tissue structures.
Clinical syndrome describing overuse tendon injuries characterized by a combination of PAIN, diffuse or localized swelling, and impaired performance. Distinguishing tendinosis from tendinitis is clinically difficult and can be made only after histopathological examination.
Authors' withdrawal or disavowal of their participation in performing research or writing the results of their study.
A fibrous cord that connects the muscles in the back of the calf to the HEEL BONE.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.

Regulation of neurotrophin-3 expression by epithelial-mesenchymal interactions: the role of Wnt factors. (1/6597)

Neurotrophins regulate survival, axonal growth, and target innervation of sensory and other neurons. Neurotrophin-3 (NT-3) is expressed specifically in cells adjacent to extending axons of dorsal root ganglia neurons, and its absence results in loss of most of these neurons before their axons reach their targets. However, axons are not required for NT-3 expression in limbs; instead, local signals from ectoderm induce NT-3 expression in adjacent mesenchyme. Wnt factors expressed in limb ectoderm induce NT-3 in the underlying mesenchyme. Thus, epithelial-mesenchymal interactions mediated by Wnt factors control NT-3 expression and may regulate axonal growth and guidance.  (+info)

AhR, ARNT, and CYP1A1 mRNA quantitation in cultured human embryonic palates exposed to TCDD and comparison with mouse palate in vivo and in culture. (2/6597)

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is developmentally toxic in many species and induces cleft palate in the C57BL/6N mouse embryo. Palatogenesis in mouse and human embryos involves homologous processes at the morphological, cellular, and molecular levels. In organ culture, mouse and human palates respond similarly to TCDD. The present study quantitates the expression of AhR, ARNT, and CYP1A1 mRNA in human embryonic palates in organ culture. Palatal tissues were exposed to 1 x 10(-10), 1 x 10(-9), or 1 x 10(-8) M TCDD or control medium and sampled at 0, 2, 4, and 6 hours for quantitative RT-PCR using a synthetic RNA internal standard. Similar measurements of CYP1A1 gene expression were collected for mouse palates cultured in this model. In human palates, AhR expression correlated with ARNT and CYP1A1 mRNA expression. TCDD induction of CYP1A1 was time- and concentration-dependent. The expression of these genes presented a uniform and continuous distribution across the group of embryos, with no subset of either high or low expressors/responders. The ratio of AhR to ARNT was approximately 4:1. AhR mRNA increased during the culture period in both treated and control subjects; however, ARNT expression was relatively constant. TCDD did not alter either AhR or ARNT expression in a consistent dose- or time-related manner. Comparison of human and mouse data showed a high correlation across species for the induction of CYP1A1. Human embryos expressed approximately 350 times less AhR mRNA than the mouse, and in earlier studies it was shown that human palates required 200 times more TCDD to produce the same effects. When the morphological, cellular, and molecular responses to TCDD between mouse and human are compared, it seems highly unlikely that human embryos could be exposed to sufficient TCDD to achieve changes in palatal differentiation that would lead to cleft palate.  (+info)

gas2 is a multifunctional gene involved in the regulation of apoptosis and chondrogenesis in the developing mouse limb. (3/6597)

The growth-arrest-specific 2 (gas2) gene was initially identified on account of its high level of expression in murine fibroblasts under growth arrest conditions, followed by downregulation upon reentry into the cell cycle (Schneider et al., Cell 54, 787-793, 1988). In this study, the expression patterns of the gas2 gene and the Gas2 peptide were established in the developing limbs of 11.5- to 14. 5-day mouse embryos. It was found that gas2 was expressed in the interdigital tissues, the chondrogenic regions, and the myogenic regions. Low-density limb culture and Brdu incorporation assays revealed that gas2 might play an important role in regulating chondrocyte proliferation and differentiation. Moreover, it might play a similar role during limb myogenesis. In addition to chondrogenesis and myogeneis, gas2 is involved in the execution of the apoptotic program in hindlimb interdigital tissues-by acting as a death substrate for caspase enzymes. TUNEL analysis demonstrated that the interdigital tissues underwent apoptosis between 13.5 and 15.5 days. Exactly at these time points, the C-terminal domain of the Gas2 peptide was cleaved as revealed by Western blot analysis. Moreover, pro-caspase-3 (an enzyme that can process Gas2) was cleaved into its active form in the interdigital tissues. The addition of zVAD-fmk, a caspase enzyme inhibitor, to 12.5-day-old hindlimbs maintained in organ culture revealed that the treatment inhibited interdigital cell death. This inhibition correlated with the absence of the Gas2 peptide and pro-caspase-3 cleavage. The data suggest that Gas2 might be involved in the execution of the apoptotic process.  (+info)

BMP7 acts in murine lens placode development. (4/6597)

Targeted inactivation of the Bmp7 gene in mouse leads to eye defects with late onset and variable penetrance (A. T. Dudley et al., 1995, Genes Dev. 9, 2795-2807; G. Luo et al., 1995, Genes Dev. 9, 2808-2820). Here we report that the expressivity of the Bmp7 mutant phenotype markedly increases in a C3H/He genetic background and that the phenotype implicates Bmp7 in the early stages of lens development. Immunolocalization experiments show that BMP7 protein is present in the head ectoderm at the time of lens placode induction. Using an in vitro culture system, we demonstrate that addition of BMP7 antagonists during the period of lens placode induction inhibits lens formation, indicating a role for BMP7 in lens placode development. Next, to integrate Bmp7 into a developmental pathway controlling formation of the lens placode, we examined the expression of several early lens placode-specific markers in Bmp7 mutant embryos. In these embryos, Pax6 head ectoderm expression is lost just prior to the time when the lens placode should appear, while in Pax6-deficient (Sey/Sey) embryos, Bmp7 expression is maintained. These results could suggest a simple linear pathway in placode induction in which Bmp7 functions upstream of Pax6 and regulates lens placode induction. At odds with this interpretation, however, is the finding that expression of secreted Frizzled Related Protein-2 (sFRP-2), a component of the Wnt signaling pathway which is expressed in prospective lens placode, is absent in Sey/Sey embryos but initially present in Bmp7 mutants. This suggests a different model in which Bmp7 function is required to maintain Pax6 expression after induction, during a preplacodal stage of lens development. We conclude that Bmp7 is a critical component of the genetic mechanism(s) controlling lens placode formation.  (+info)

Induction of Sarcophaga central nervous system remodeling by 20-hydroxyecdysone in vitro. (5/6597)

Proliferation and apoptosis of neural cells were found to be induced simultaneously when larval brains of Sarcophaga peregrina were cultured in the presence of 20-hydroxyecdysone (20-HE) for 24 h. The locations of proliferating cells and apoptotic cells in the brain hemispheres were different. The morphology of brains exposed to 20-HE for a short period proceeded to change sequentially when culture was continued for 2 days even in the absence of 20-HE. These changes mainly consisted of enlargement of the brain hemispheres and extension of the interval between two hemispheres, which closely paralleled the morphological changes of brains that occur in the early pupal stage, suggesting that ecdysteroid alone is sufficient to induce the remodeling of the central nervous system of holometabolous insects. Synthesis of a protein with a molecular mass of 66 kDa was shown to be selectively repressed when brains were cultured in the presence of 20-HE.  (+info)

Modulation of the baboon (Papio anubis) uterine endometrium by chorionic gonadotrophin during the period of uterine receptivity. (6/6597)

This study was undertaken to determine the modulation of uterine function by chorionic gonadotrophin (CG) in a nonhuman primate. Infusion of recombinant human CG (hCG) between days 6 and 10 post ovulation initiated the endoreplication of the uterine surface epithelium to form distinct epithelial plaques. These plaque cells stained intensely for cytokeratin and the proliferating cell nuclear antigen. The stromal fibroblasts below the epithelial plaques stained positively for alpha-smooth muscle actin (alphaSMA). Expression of alphaSMA is associated with the initiation of decidualization in the baboon endometrium. Synthesis of the glandular secretory protein glycodelin, as assessed by Western blot analysis, was markedly up-regulated by hCG, and this increase was confirmed by immunocytochemistry, Northern blot analysis, and reverse transcriptase-PCR. To determine whether hCG directly modulated these uterine responses, we treated ovariectomized baboons sequentially with estradiol and progesterone to mimic the hormonal profile of the normal menstrual cycle. Infusion of hCG into the oviduct of steroid-hormone-treated ovariectomized baboons induced the expression of alphaSMA in the stromal cells and glycodelin in the glandular epithelium. The epithelial plaque reaction, however, was not readily evident. These studies demonstrate a physiological effect of CG on the uterine endometrium in vivo and suggest that the primate blastocyst signal, like the blastocyst signals of other species, modulates the uterine environment prior to implantation.  (+info)

Arterial damage induced by cryopreservation is irreversible following organ culture. (7/6597)

OBJECTIVES: The aim of the present study was to investigate the changes which occur to the arterial wall following cryopreservation and thawing and to determine whether these changes are reversible after a week of culture in an organ bath. MATERIALS AND METHODS: Rat iliac arterial segments were cryopreserved. Once thawed, the arterial segments were cultured for a period of 0, 1, 2, 4 or 7 days. Freshly isolated rat iliac vessels cultured for 7 days served as the control group. Evaluation was made of ultrastructural changes, the expression of metalloproteinase activity (MMP-1, MMP-3 and MMP-9) and the apoptotic state of cells. RESULTS: The freezing-thawing process induced damage to the arterial segments compared to fresh control vessels. After 1 week of culture, arteries showed a high degree of tissue degeneration. Only a few individual endothelial cells remained on the luminal surface. There was a gradual increase in the proportion of apoptotic cells. The sequential expression of MMP-1 during the first 2 days and subsequent expression of MMP-3 and MMP-9 were of most significance. CONCLUSIONS: Cryopreservation induced damage to the vessels which could not be reversed by organ culture. The changes observed in the expression of metalloproteinases may be indicative of the degenerative process which occurs in the extracellular matrix.  (+info)

Oxidized derivatives of 7-dehydrocholesterol induce growth retardation in cultured rat embryos: a model for antenatal growth retardation in the Smith-Lemli-Opitz syndrome. (8/6597)

7-Dehydrocholesterol accumulates in fetuses affected by the Smith-Lemli-Opitz syndrome as a result of a deficit in the ultimate step of cholesterol synthesis catalyzed by Delta7 reductase. Rat embryos explanted at gestation day 10 and cultured for 48 h in the presence of the Delta7 reductase inhibitor AY 9944 were used as a model to discriminate between the beneficial effect of supplementation with cholesterol and the deleterious effect of supplementation with 7-dehydrocholesterol. Cholesterol supplementation in the form of mixed cholesterol/lecithin liposomes added to serum serving as the culture medium restores the growth of embryos which is markedly decreased in the presence of the inhibitor. 7-Dehydrocholesterol under identical conditions does not restore growth and impairs the beneficial effect of cholesterol added simultaneously. UV-photooxidation of 7-dehydrocholesterol-supplemented culture medium enhances its embryotoxicity, which suggests uptake by the embryo of toxic by-products formed from 7-dehydrocholesterol. By contrast photooxidation of cholesterol-supplemented culture medium does not induce embryotoxicity. alpha-Tocopherol reduces the toxicity of photooxidized 7-dehydrocholesterol supplementing the culture medium. We conclude that 7-dehydrocholesterol does not fulfill the cholesterol requirement of the developing embryos and exerts an additional embryotoxic effect probably via oxidized by-products. This could explain the antenatal growth retardation of SLOS by a blockage of the maternal compensatory cholesterol influx.  (+info)

Whole organ culture of the intervertebral disc (IVD) preserves the native extracellular matrix, cell phenotypes, and cellular-matrix...
Project 1 work was focused on using multi-plexed assays for high-content imaging and high-throughput transcriptomic analyses using iCell cardiomyocyte organotypic culture model and other cell types derived from iPS cells. We have completed testing 140 compounds selected in consultation with FDA, NTP and EPA-NCCT in a population-based in vitro iPSC-derived cell model with iCell cardiomyocytes from 43 normal donors. Several manuscripts detailing the outcomes have been published or are in submission. Using these data from this in vitro screening, and data from human clinical trials, we demonstrated that in vitro human iPSC-derived cardiomyocyte model accurately predicts clinical concentration-QTc relationships. This finding confirms our central hypothesis that human induced pluripotent stem cell (iPSC)-derived cardiomyocyte cultures constitute an effective organotypic culture model for predictive toxicity screening. We also showed that iPSC-derived cardiomyocytes are a highly reproducible in vitro ...
Organ Culture Studies of Murine Oral Mucosa with Particular Emphasis on Epithelial Proliferation and Connective Tissue Mast Cells in the Presence and Absence of Oestrogen ...
НИИ атеросклероза: научные исследования, публикации сотрудников института (abstracts, full-text.), дискуссионный клуб, посвященный вопросам механизмов атерогенеза.
The liver, the largest organ of the human body, is a multifunctional organ with various metabolic activities that plays a fundamental role in maintaining the body and in sustaining life. Although the liver has great regenerative capacity and recovery, the damage caused by chronic diseases such as cancer or viral infections can lead to permanent loss of liver function. Studies on the mechanism of liver disease, have focused on the selection of cell and tissue culture techniques, including strategies based on |i|in vitro|/i| models. The organ culture is a promising tool for the study of liver diseases, because it can mimic the complex of the microenvironment |i|in vivo|/i| using a three-dimensional model of human liver tissue. These models allow a better study of the specific functions of the liver. In this context, we have analyzed the development of a hepatocarcinoma, obtained by inoculating a murine hepatocarcinoma cell line, Hepa 1/A1s, in the liver of 10 mice of the strain C57BL / 6. After 20 days
All imaging and electrophysiology measurements were performed in Tyrodes buffer containing the following (in mm): 125 NaCl, 2.5 KCl, 3 CaCl2, 1 MgCl2, 15 HEPES, and 30 glucose, pH 7.3. For HEK cell measurements, 2-aminoethoxydiphenyl borate (100 μm; Sigma-Aldrich) was added to block endogenous gap junctions. Measurements at 34°C were performed by perfusing in Tyrodes buffer at 1 ml/min while maintaining elevated temperature with an inline heater (Warner Instruments) and an objective heater (Bioptechs).. Filamented glass micropipettes (WPI) were pulled to a tip resistance of 4-8 MΩ and filled with internal solution containing the following (in mm): 125 potassium gluconate, 8 NaCl, 0.6 MgCl2, 0.1 CaCl2, 1 EGTA, 10 HEPES, 4 Mg-ATP, and 0.4 Na-GTP, pH 7.3, adjusted to 295 mOsm with sucrose. Pipettes were positioned with a Sutter Instruments MP285 manipulator. Whole-cell, voltage and current-clamp recordings were acquired using an Axopatch 700B amplifier (Molecular Devices), filtered at 2 kHz ...
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Organotypic slice cultures are the in vitro method of choice for applications requiring long‐term survival of the preparation and a high degree of cellular differentiation and organization resembling that of the original tissue
We established a serum-free organ culture system of isolated single vibrissa rudiments taken from embryonic day 13 mice. This… Expand ...
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Pancreatic cancer is characterized by an intense stromal reaction. Reproducible three-dimensional in vitro systems for exploring interactions of the stroma with pancreatic cancer cells have not previously been available, prompting us to develop such a model. Cancer cells were grown on collagen/Matri …
Faramarz Dehghani is the author of these articles in the Journal of Visualized Experiments: Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells, Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells
The Division is working on the characterization of the genetic response of organisms and their cells to external signals (growth factors, inflammation-related cytokines, carcinogens and tumor promoters), employing genetically modified mouse models (which replicate features of human diseases) and cell- and organ cultures derived thereof. We want to define and characterize altered gene functions, which result in imbalanced signaling pathways and downstream genetic programs that form the basis for acquired capabilities of uncontrolled cell growth, evasion of apoptosis, sustained angiogenesis and, finally, enhanced tissue invasion and metastasis. To date, we have applied genetically modified animal models, in vitro organ systems, and genome-wide expression analyses in order to decipher the individual functions of AP-1 subunits. Our study of individual AP-1 subunits with regard to their regulation, as well as the identification of distinct targets genes and genetic programs critically involved in ...
As many of you know, my experimental background is in hippocampal culture. Recently, I attended a hippocampal slice culture workshop given by members of the Hayashi lab here at MIT. I never really knew too much about the pros and cons of slice culture. After seeing the technique, I wrote up a little summary of…
A whole functional organ has been successfully grown in animals for the first time; a group of Scottish researchers created a group of cells which, when
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The main objective of the global In Vitro Organ Preservation market report published by OrbisPharmaReports is to identify the positioning of top players in the In Vitro Organ Preservation market along with their company profiles. A statistical analysis comparing the top players and potential for new entrants including mergers and efforts taken to adopt AI, digitization and automation are explained in the In Vitro Organ Preservation market report. The report enlists the top players along with their market revenues. Data in the In Vitro Organ Preservation report is provided through graphs, pie charts and figures for better understanding of the customers. The In Vitro Organ Preservation report also includes trends and expected opportunities widening the global In Vitro Organ Preservation market. Request a sample of In Vitro Organ Preservation Market report @ https://www.orbispharmareports.com/sample-request/70788. The In Vitro Organ Preservation report documented by OrbisPharmaReports implies an ...
TY - JOUR. T1 - A novel organ culture model of a joint for the evaluation of static and dynamic load on articular cartilage. AU - Lin, Y. C.. AU - Hall, A. C.. AU - Simpson, A. H.R.W.. PY - 2018/3/1. Y1 - 2018/3/1. N2 - Objectives: The purpose of this study was to create a novel ex vivo organ culture model for evaluating the effects of static and dynamic load on cartilage. Methods: The metatarsophalangeal joints of 12 fresh cadaveric bovine feet were skinned and dissected aseptically, and cultured for up to four weeks. Dynamic movement was applied using a custom-made machine on six joints, with the others cultured under static conditions. Chondrocyte viability and matrix glycosaminoglycan (GAG) content were evaluated by the cell viability probes, 5-chloromethylfluorescein diacetate (CMFDA) and propidium iodide (PI), and dimethylmethylene blue (DMMB) assay, respectively. Results: Chondrocyte viability in the static model decreased significantly from 89.9% (sd 2.5%) (Day 0) to 66.5% (sd 13.1%) ...
TY - JOUR. T1 - Dentate development in organotypic hippocampal slice cultures from p35 knockout mice. AU - Wenzel, H. Jürgen. AU - Tamse, Catherine T.. AU - Schwartzkroin, Philip A. PY - 2006/12. Y1 - 2006/12. N2 - Abnormal brain development, induced by genetic influences or resulting from a perinatal trauma, has been recognized as a cause of seizure disorders. To understand how and when these structural abnormalities form, and how they are involved in epileptogenesis, it is important to generate and investigate animal models. We have studied one such model, a mouse in which deletion of the p35 gene (p35-/-) gives rise to both structural disorganization and seizure-like function. We now report that aberrant dentate development can be recognized in the organotypic hippocampal slice culture preparation generated from p35-/- mouse pups. In these p35-/- cultures, an abnormally high proportion of dentate granule cells migrates into the hilus and molecular layer, and develops aberrant dendritic and ...
Corning Matrigel Matrix for Organoid Culture is a solubilized basement membrane preparation that has been optimized for organoid culture. Verified to support both mouse and human organoid growth and differentiation from healthy and/or diseased tissue, each lot has a specific elastic modulus value indicating the stiffness of the gel formed and is qualified to form stable 3D domes commonly used in organoid culture protocols. Available in 10mL LDEV-free, Phenol Red-free format. Corning Matrigel Matrix for Organoid culture is extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors. Quality • Tested for ability to form and maintain stable 3D domes/droplets on multi-well plates. • Elastic Modulus (matrix stiffness) measured using a rheometer. • Protein concentrations determined by Lowry method • Mouse colonies
article{1fdd16d8-cade-46c6-b3bf-0719173f035c, abstract = {Both brain-derived neurotrophic factor (BDNF) and the serotonin receptor 2A (5-HT2A) have been related to depression pathology. Specific 5-HT2A receptor changes seen in BDNF conditional mutant mice suggest that BDNF regulates the 5-HT2A receptor level. Here we show a direct effect of BDNF on 5-HT2A receptor protein levels in primary hippocampal neuronal and mature hippocampal organotypic cultures exposed to different BDNF concentrations for either 1, 3, 5 or 7 days. In vivo effects of BDNF on hippocampal 5-HT2A receptor levels were further corroborated in (BDNF +/-) mice with reduced BDNF levels. In primary neuronal cultures, 7 days exposure to 25 and 50 ng/mL BDNF resulted in downregulation of 5-HT2A, but not of 5-HT1A, receptor protein levels. The BDNF-associated downregulation of 5-HT2A receptor levels was also observed in mature hippocampal organotypic cultures, excluding confounding effects of BDNF on immature tissue. BDNF +/- mice ...
The BH3 interacting-domain death agonist (BID) is a pro-apoptotic protein involved in death receptor-induced and mitochondria-mediated apoptosis. Recently, it has also been suggested that BID is involved in the regulation of inflammatory responses in the central nervous system. We found that BID deficiency protected organotypic hippocampal slice cultures in vitro from neuronal injury induced by oxygen-glucose deprivation. In vivo, BID-knockout (KO) mice and WT mice were subjected to 60 minutes of transient middle cerebral artery occlusion to induce focal cerebral ischemia, and allowed to recover for 24 hours. Infarct volumes and functional outcome were assessed and the inflammatory response was evaluated using immunofluorescence, Western blotting, quantitative PCR and Mesoscale multiplex analysis. We observed no difference in the infarct volume or neurological outcome between BID-KO and WT mice. The inflammatory response was reduced by BID deficiency as indicated by a change in microglial/leukocyte
Organ culture is a development from tissue culture methods of research, the organ culture is able to accurately model functions of an organ in various states and conditions by the use of the actual in vitro organ itself. Parts of an organ or a whole organ can be cultured in vitro. The main objective is to maintain the architecture of the tissue and direct it towards normal development. In this technique, it is essential that the tissue is never disrupted or damaged. It thus requires careful handling. The media used for a growing organ culture are generally the same as those used for tissue culture. The techniques for organ culture can be classified into (i) those employing a solid medium and (ii) those employing liquid medium. In April 2006, scientists reported a successful trial of seven bladders grown in-vitro and given to humans. A bladder has been cultured by Anthony Atala of the Wake Forest Institute for Regenerative Medicine in Winston-Salem, North Carolina. A jawbone has been cultured at ...
Understanding how probiotics, prebiotics or postbiotics work in preclinical models that resemble the human situation can allow a rational choice of the different strains or compounds for clinical and/or commercial use according to the pathological condition to which they are targeted. Indeed, we recently demonstrated that different Lactobacilli species can be classified as either immunogenic or tolerogenic.17 Testing of different probiotics has often been performed on isolated immune cells or peripheral blood mononuclear cells.21 However, particularly in the gut, the interaction of bacteria with the mucosa is an unusually complex event requiring attachment or degradation of mucus, competition with the microbiota and resistance to antimicrobial peptides.13 In addition, the function of immune cells is influenced by the local microenvironment and this is required to preserve intestinal homeostasis.4-22 Indeed, we have shown that signals from intestinal epithelial cells can drive the ...
Studies of the most immature T cell progenitors in the human thymus have been hampered by the lack of markers and assays that define these cells. In this report we used a novel human fetal thymic organ culture system to determine the potential of T cell precursors isolated from human postnatal thymus, to differentiate into CD3+ thymocytes, and to investigate early stages of human T cell development. It was found that thymocytes that lack the markers CD3, CD4, and CD8 (triple negative [TN]) can differentiate in an allogeneic organotypic thymic culture. The capacity of TN thymocytes to differentiate was exclusively confined to the CD34+ population. CD34- TN thymocytes failed to differentiate in this system. In contrast, cloned lines of CD3- thymocytes could only be established from CD34- TN thymocytes. Five subsets of CD3- thymocytes were found with the following phenotype: CD1-TN, CD1+TN, CD1+CD4+CD8-, CD1+CD4+CD8 alpha+ beta-, and CD1+CD4+CD8 alpha beta+. These subpopulations expressed ...
A novel in vitro culture system of organotypic human skin explants interfacing with external fixator pins is presented. The system was used to observe changes in skin morphology on the skin at the pin
Indirect evidence suggests that stimulation of α1-adrenergic receptors (ARs) increases smooth muscle cell (SMC) growth in the growing and adult artery and worsens atherosclerosis and restenosis after balloon injury. In support of a direct adrenergic effect, we have previously shown that α1D-AR stimulation induces SMC hypertrophy in cell and vessel organ culture. Because interactions between α1-ARs and peptide growth factors may be important in normal and pathological SMC growth, herein we examined regulation of α1D-AR expression by growth factors. Platelet-derived growth factor (PDGF)-BB dose- and time-dependently lowered α1D mRNA in cultured quiescent SMCs (e.g., 58% inhibition at 20 ng/ml, 24 h, p , .05), whereas other α1-AR transcripts were unaffected. This same selective effect was seen in the medial layer of aorta in ex vivo organ culture. However, PDGF-AA, insulin-like growth factor-1, insulin, epidermal growth factor, endothelin, histamine, and serotonin had no effect, whereas ...
The processes responsible for epithelial spreading during wound healing and embryonic morphogenesis were investigated in an organ culture model in which an epithelial tissue (chick embryo pigmented retinal epithelium) spread over the surface of an aggregate of mesenchyme cells (chick embryo cardiac mesenchyme). The heart mesenchyme aggregate is differentiated into a core of stellate cells associated with a fibronectin-poor matrix surrounded by a cortical zone, 2-5 cells in thickness, of flattened cells embedded in a fibronectin-rich extracellular matrix. Envelopment of the mesenchyme aggregate is accompanied by a movement of the cells and the fibronectin-rich extracellular matrix of the cortex over the core tissue in advance of the spreading pigmented retina tissue. Three distinct processes were identified as contributing to epithelial spreading in this system: (1) active migration of the pigmented retinal epithelium; (2) active contraction of the cortical cells of the mesenchyme aggregate to ...
Re‐formation or preservation of functional, electrically active neural networks has been proffered as one of the goals of stem cell-mediated neural therapeutics
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Top 10 tissues for NP_776216 (Homo sapiens, RefSeq): peripheral blood anergic B-cell, peripheral blood CD4 resting T-cell (unspecified), peripheral blood CD4 memory regulatory T-cell, peripheral blood CD4 naive regulatory T-cell, tumor derived CD4 T-cell (unspecified), peripheral blood CD4 memory T-cell (unspecified), peripheral blood CD8 activated T-cell (unspecified), tonsillar activated T-cell (unspecified), bone marrow CD4 T-cell (unspecified), gingival epithelium organotypic model
The aim in developing this approach to the study of implantation has been to establish a method which will permit the investigation of the process extracorporeally, thus providing conditions that are more readily controllable than those obtainable in an experimental animal. Shaffers method of organ culture has been adapted to the needs of the present investigation and it has been found that somewhat more than half of the rabbit blastocysts, explanted on strips of endometrium and incubated on them as combined explants, became implanted within 48 to 72 h. ...
Get this from a library! Plant cell, tissue and organ culture : fundamental methods. [O L Gamborg; Gregory C Phillips;] -- A manual providing all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis and gene transfer. Also ...
Complete cell culture medium for establishing three-dimensional (3D) intestinal organoid cultures from isolated human intestinal crypts or stem cells.
Completed, defined cell culture medium for establishing three-dimensional (3D) intestinal organoid cultures from isolated mouse intestinal crypts.
Recent sequencing efforts have revealed that cancer types carry different patterns of mutation, suggesting that what causes the damage or how it is repaired varies from tissue to tissue. Our research will therefore aim to answer fundamental questions relating to the maintenance of genome integrity in a physiological setting: are there tissue-specific mutagenic processes? What are the endogenous metabolites that damage DNA? And what are the DNA repair/tolerance mechanisms that lead to mutagenesis in vivo? Our lab applies tools from the fields of DNA repair and stem cell biology, as well as ESC and organoid culture, genomics and mouse genetics to interrogate these fundamental questions.. ...
Fibroblasts transformed into induced thymic epithelial cells (iTEC) in vitro (left, iTEC shown in green). iTEC transplanted onto the mouse kidney forms an
from the top of the slice culture, with or without additional illumination from the bottom, we obtained good cellular resolution of ...
Integrated approaches using different in vitro methods in combination with bioinformatics can (i) increase the success rate and speed of drug development; (ii) improve the accuracy of toxicological risk assessment; and (iii) increase our understanding of disease. Three-dimensional (3D) cell culture models are important building blocks of this strategy which has emerged during the last years. The majority of these models are organotypic, i.e., they aim to reproduce major functions of an organ or organ system. This implies in many cases that more than one cell type forms the 3D structure, and often matrix elements play an important role. This review summarizes the state of the art concerning commonalities of the different models. For instance, the theory of mass transport/metabolite exchange in 3D systems and the special analytical requirements for test endpoints in organotypic cultures are discussed in detail. In the next part, 3D model systems for selected organs liver, lung, skin, brain are ...
Local drug delivery by coronary stents is of current research interest. Organ culture of human vascular tissue is a model of intimal hyperplasia. We report an ex vivo organ culture model of stented vessels. This allows stent-artery interactions to be studied in living tissue. The recognized anti-restenosis agent paclitaxel was chosen to test the organ culture model. Mammary artery specimens were cultured closed (i.e. without opening them flat) for 72h. Phosphocholine-coated stents, half of them loaded with the anti-restenosis drug paclitaxel, were implanted. The absorption and elution characteristics of paclitaxel were established. Artery tissue remained viable at 72h when cultured closed, despite stent implantation. Specimens developed smooth muscle cell proliferation. The stents absorbed up to 127±29μg of paclitaxel, with a biphasic elution curve. A mean of 13% of the absorbed paclitaxel remained after a 24h perfusion. In mammary artery, these paclitaxel stents reduced or abolished smooth ...
AMSBIO announces an extended and updated edition of its popular Organoid Culture Handbook. Launched at the EMBO Symposium on Organoids: Modelling Organ Development and Disease in 3D Culture, the new 24-page handbook includes valuable information about the latest available products, protocols, example results and research paper citations. Within the protocols section detailed information is provided on a general submerged method for organoid culture, crypt organoid culture techniques...
Until fairly recently very little was known about the receptors, ligands, and molecular signals necessary for thymocyte emigration. Earlier work showed that expression of the catalytic subunit of pertussis toxin in thymocytes inhibited their emigration, pointing toward the involvement of a Gαi protein-coupled receptor (31). Studies of egress from fetal thymic organ cultures suggested that CCR7 plays a role in the neonatal period (32), but in the adult, thymocytes emigrate independently of CCR7 despite having an impaired cortex-to-medulla migration (1, 2, 14). Chemorepulsion or fugetaxis has also been proposed to have a role in thymic emigration. In in vitro assays, CXCR4-expressing thymocytes move away from the thymus in a stromal cell-derived factor 1 (SDF-1)-dependent manner (33). The disruption of SDF-1/CXCR4 interactions by genetic deficiency or pharmacological antagonism with AMD3100 led to decreased migration in fetal thymic organ culture. In vivo treatment with AMD3100 in newborn mice ...
The WHO estimates that up to 23% of the global burden of cardiovascular diseases, a leading cause of death, is attributable to environmental chemicals. Methods for assessment of cardiac safety of non-pharmaceutical agents lag behind the traditional health hazards of concern to human health (carcinogenicity, mutagenicity, reproductive toxicity, etc.). The long-term objective of the Center is to advance chemical risk assessment by establishing and validating effective, accurate and fiscally responsible means for identifying/characterizing cardiac chemical hazards.. Recent advances in stem cell research and establishment of robust protocols for culturing, distribution and phenotyping holds promise for development of a functional cardiac OCM for modeling cardiovascular disease and testing for chemical hazards. The central hypotheses of this proposal are that: (i) stem cell-derived cardiomyocyte cultures constitute an effective OCM for predictive toxicity screening of environmental chemicals; (ii) a ...
Discussion about Covid19/WORLD:6.575.000/USA:1.902.000/RIOTS ARE SUPER SPREADERS P9441/ALSO MILD LEAVES LONG-TERM ORGAN DAMAGE P9451/US CURVE P9464 [Page 9337] at the GodlikeProductions Conspiracy Forum. Our topics include Conspiracy Theory, Secret Societies, UFOs and more!
To investigate the changes in COX-2 activity and expression in bypass conduits after CABG surgery, we studied isolated segments of SV and IMA from the same patients using an organ culture system. After 2 consecutive days in culture, segments of SV and IMA released prostanoids in the order of PGE2≥prostacyclinTXA2, and these segments contained detectable COX-2 mRNA. These results are consistent with the reported high expression of prostacyclin synthase and low expression of TX synthase in vascular smooth muscle22 23 and the ability of PGE2 to be formed nonenzymatically.24 Interestingly, we did not detect mRNA for COX-2 in freshly removed SV or IMA, indicating that vessels from patients, even those with underlying coronary artery disease, do not have a basal expression of COX-2 at the time of surgery. In animal models, mild physical trauma causes COX-2 induction in vivo in rat carotid arteries.9 Thus, the induction of COX-2 we observed in the SV and IMA after 48 hours in organ culture is ...
The aortic valve functions in a complex mechanical environment which leads to force-dependent cellular and tissue responses. Characterization of these responses provides a fundamental understanding of valve pathogenesis. The aim of this work was to study the biological characteristics of native porc …
1) Vision Training Grant - John Clark, Director. Multidisciplinary training in basic science and clinical aspects of vision research is conducted at both the pre- and post-doctoral levels. Faculty in 9 departments in the School of Medicine and the School of Arts and Sciences are preceptors for pre- and post-doctoral trainees in structural biology, cell and molecular biophysics, visual function and systems neurobiology using a variety of model systems in vertebrate animals and selected cell and organ culture systems. Vision training will emphasize modern technological approaches to clinical problems in the visual system in both normal and abnormal conditions. Interdisciplinary interactions that will develop new understanding of the scientific basis for important clinical problems in vision are encouraged.. 2) Development Biology Training Grant - David Raible Director. The program provides graduate work in developmental biology and supporting areas, including training in teaching, scientific ...
Chinese Journal of Cell Biology June, 2004 Vol.26 No.3 The comparision of organotypic culture spinal cord and internal spinal cord Li Chun Yan, Wang Xiaojuan Read more: en/upfiles/pdf/The_comparision_of_organotypic_culture_spinal_cord_and_internal_sp
Spontaneous neuronal activity prevails in virtual all brain regions in vivo as well as brain tissues in vitro. But its function, influence, or spatiotemporal patterns are ill-defined. In acute brain slice preparations, however, the level of spontaneous activity is reduced because of massively amputated neurites during slicing. Organotypic slice cultures self-restore neurites and synaptic connections and remodel the network complexity through the intrinsic rules of neural plasticity. Therefore, cultured slices could offer a unique opportunity for investigating some aspect of spontaneous activity. Here we focused on hippocampal networks and evaluated the similarity of spontaneous activity among acute slices, cultured slices and in vivo networks. Using functional multineuron calcium imaging and cell-attached patch-clamp recordings, we found that the mean firing rates of individual neurons of slice cultures did not differ from those of the in vivo hippocampus and were higher than those of acute ...
The productive program of the human papillomaviruses takes place in terminally differentiating squamous epithelia. In this chapter, we provide the protocols for robust production of HPV-18 in organotypic cultures of early passages of primary human keratinocytes. A critical step is the generation of genomic HPV plasmids in vivo by using Cre-loxP-mediated excisional recombination from a vector plasmid. We discuss the rationale for this approach. This system produces high yields of infectious virus and facilitates genetic analyses of HPV protein functions and their regulation in the context of recapitulated host tissue environment.. ...
The mechanisms by which human stem cells self‐organise into brain‐like tissues in 3D organoid culture are poorly understood. In this issue of The EMBO Journal, Renner and Lancaster et al demonstrate that in the absence of external stimuli, human cerebral organoids develop large forebrain structures that display specific landmarks of spatial and temporal patterning, including signalling centres producing known morphogens. The generation of cerebral organoids is therefore likely to reflect normal brain development.. See also: M Renner et al (May 2017) ...
The mechanisms by which human stem cells self‐organise into brain‐like tissues in 3D organoid culture are poorly understood. In this issue of The EMBO Journal, Renner and Lancaster et al demonstrate that in the absence of external stimuli, human cerebral organoids develop large forebrain structures that display specific landmarks of spatial and temporal patterning, including signalling centres producing known morphogens. The generation of cerebral organoids is therefore likely to reflect normal brain development.. See also: M Renner et al (May 2017) ...
Cha nges i n optical atte nuatio n, releva nt to cytochrome oxidase, of the rat bo ne periosteal tissue i n expla nted culture a nd huma n neuro nal cells i n three-dime nsio nal agarose co nstructs h
To address the current demand for 3D and organotypic culture reagents we have recently inaugurated our Organoid Resource Lab (ORL). Trevigens ORL has developed essential reagents such as our Cultrex® R-Spondin1 expressing cell line and our Organoid Harvesting Solution. We also offer a new line of organoid qualified matrices, BME type 2 and BME-R1, that are tested and qualified using enteroid progenitor cells.. ...
... in the organ cultures that they had collected, with her "new, improved techniques", he was skeptical. Tyrrell's team sent ... This organ culture system meant they did not have to depend on human volunteers to do research on these viruses. They wanted a ... The Swedish professor Bertil Hoorn could make all Tyrrell's respiratory viruses in organ cultures of cells from the human ... who was working on a new organ culture system. Tyrrell's team had been attempting to detect the presence of rhinoviruses in ...
This is particularly important in hepatocyte cultures because the liver is a highly vascularized organ. One study cultured ... scaffold techniques and scaffold-free techniques. Scaffold techniques include the use of solid scaffolds, hydrogels and other ... Haycock JW (2011). 3D cell culture: a review of current approaches and techniques. Methods in Molecular Biology. 695. pp. 1-15 ... Lastly, 3D cell cultures have greater stability and longer lifespans than cell cultures in 2D. This means that they are more ...
Tissue and Organ Culture. 105 (2): 149-158. doi:10.1007/s11240-010-9863-9. ISSN 0167-6857. S2CID 24101347. 和宏, 富士原; 豊樹, 古在; 一郎 ... to ex vitro transition of tissue cultured potato plantlets grown with or without sucrose using metabolic profiling technique". ... Photoautotrophic tissue culture is defined as "micropropagation without sugar in the culture medium, in which the growth or ... Tissue and Organ Culture. 123 (1): 121-132. doi:10.1007/s11240-015-0820-5. ISSN 0167-6857. S2CID 16036459. Martins, João Paulo ...
... tissue and organ culture (In Vitro Culture Techniques: The Biotechnological Principles). Desirable requirements are (i) air ... Cellulose substrate Rock wool Requirements for animal cell and tissue culture are the same as described for plant cell, ... Moreover, plastic beads of polystyrene, sephadex and polyacrylamide are also available for cell growth in suspension culture. ... "Requirements for Animal Cell and Tissue Culture - Animal Biotech". biocyclopedia.com. Retrieved 2018-03-13. "Micro-vegetable ...
In biology, explant culture is a technique to organotypically culture cells from a piece or pieces of tissue or organ removed ... cartilage explant culture, or blastocyst implant culture. Historically, explant culture has been used in several areas of ... Explant culture can also refer to the culturing of the tissue pieces themselves, where cells are left in their surrounding ... "Explant Culture for Developmental Studies , Protocol". www.jove.com. Retrieved 2017-05-25. "Explant Culture of Neural Tissue". ...
The most remarkable and fundamental method on cell culture is cell hybridization. An organ culture is an excellent experimental ... stained tissues to observation of living cells attracted great enthusiasm when the techniques were first developed, although ... Her contributions to science included the development of experimental methods in organ culture, tissue culture, and cell ... During that time, she also maintained an active research program in tissue and organ culture. Although the laboratory was never ...
The experimental units can for example be cell-cultures, tissues, organs or living animals. The biological response may be ... Statistical techniques such as optimization by Maximum Likelihood can be used to calculate estimates of the parameters. Of ... ISBN 0-02-844640-2 Govindarajulu, Z. (2001). Statistical Techniques in Bioassay, 2nd revised and enlarged edition, Karger, New ... usually by interpolation techniques, and in many cases to express the potency/activity of the test preparation(s) relative to a ...
... a retired USDA entomologist who worked in Mississippi and developed insect organ culture techniques and detailed the anatomy of ...
... operates its own laboratory for cell and organ culture. It is the coordinator of SkinTreat (FP-7) and a partner in ... employs minimally invasive techniques to harvest mud and minerals. Ingredients are not tested on animals and Ahava products are ... irradiated human skin organ cultures. There is a controversy about whether Ahava is in breach of international law, due to the ...
... is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile ... The Plant Tissue Culture Information Exchange lists many commercial tissue culture labs. Since plant tissue culture is a very ... Certain techniques such as meristem tip culture can be used to produce clean plant material from virused stock, such as ... Different techniques in plant tissue culture may offer certain advantages over traditional methods of propagation, including: ...
The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. ... Cell Culture Basics - Introduction to cell culture, covering topics such as laboratory set-up, safety and aseptic technique ... Plant cell cultures are typically grown as cell suspension cultures in a liquid medium or as callus cultures on a solid medium ... These are generally performed using tissue culture methods that rely on aseptic technique. Aseptic technique aims to avoid ...
Ovule culture or in ovolo embryo culture is a modified technique of embryo rescue whereby embryos are cultured while still ... Both plant and animal cells, tissues and organ culture is possible in artificial nutrient medium in controlled laboratory ... Embryo rescue is one of the earliest and successful forms of in-vitro culture techniques that is used to assist in the ... Ovary or pod culture, on the other hand employs the use of an entire ovary into culture. It becomes necessary to excise the ...
The media used for a growing organ culture are generally the same as those used for tissue culture. The techniques for organ ... The following are four techniques employed for embryonic organ culture. The following are general steps in organ culture on ... Organ culture is a development from tissue culture methods of research, the organ culture is able to accurately model functions ... Embryonic organs generally grow well on agar, but adult organ culture will not survive on this medium. The culture of adult ...
... evidence of a potent activity on neuronal development in primary cell cultures". Microscopy Research and Technique. 52 (5): 484 ... The subcommissural organ (SCO) is one of the circumventricular organs of the brain. It is a small glandular structure that is ... Rodríguez EM, Rodríguez S, Hein S (April 1998). "The subcommissural organ". Microscopy Research and Technique. 41 (2): 98-123. ... SCO-spondin and F-spondin share a similar pattern of expression in the floor plate, flexural organ and subcommissural organ and ...
"A Closer Look: Sound Generation and Hearing - Bee Culture". 22 February 2016. Strauß, Johannes; Lakes-Harlan, Reinhard (2014). ... Microscopy Research and Technique. 63 (6): 315-337. doi:10.1002/jemt.20051. ISSN 1097-0029. PMID 15252876. " ... A tympanal organ (or tympanic organ) is a hearing organ in insects, consisting of a membrane (tympanum) stretched across a ... Hymenoptera (bees, wasps, ants, etc.) do not have a tympanal organ, but they do have a Johnston's organ. Tympanal organs occur ...
Using 3D cell culture techniques enables scientists to recreate the complex extracellular matrix, ECM, found in in vivo to ... Artificial bone, skin, uterus, kidney Biomechatronics Biomedical Engineering Decellularization Organ transplant Organ culture ... An artificial organ is a human made organ device or tissue that is implanted or integrated into a human - interfacing with ... RFID tags). Organ chips are devices containing hollow microvessels filled with cells simulating tissue and/or organs as a ...
The Egyptian culture and religion, moreover, add to the demand of organs from living donors, creating a black market that suits ... The rural labor can not find jobs requiring special techniques or good education. A more accessible option is to work in the ... patients in need of organs tend to resist accepting organs from the deceased. Many patients hesitate to ask for organs from a ... These foreign patients form the demand for organs in Egypt through transplant tourism. Due to the Egyptian culture dated back ...
However, as a culture becomes larger and more complex, such as the case with engineered organs and whole tissues, other ... It might be possible to print organs, or possibly entire organisms using additive manufacturing techniques. A recent innovative ... Tissue engineered cultures also present additional problems in maintaining culture conditions. In standard cell culture, ... An artificial organ is an engineered device that can be extra corporeal or implanted to support impaired or failing organ ...
... (MP) is a technique used in organ transplantation as a means of preserving the organs which are to be ... Carrel A, Lindbergh CA (1935). "The culture of whole organs". Science. 81 (2112): 621-623. Bibcode:1935Sci....81..621C. doi: ... Kiser used this technique to achieve successful 7 hours in vitro storage of a dog kidney, when the kidney had been flushed at 5 ... He based his technique on the observation by Keller that the loss of electrolytes from a kidney during storage could be ...
Aaron Turner - guitars, electronics (2000-present) Faith Coloccia - piano, synthesizer, organ (2010-present) Kevin Micka - ... electronics Submarine Immersion Techniques Vol. 1 (full-length, 2000) Gettin' Sentimental (full-length, 2002) Edward's Lament ( ... House of Low Culture @ missinglink.net.au Archived 2008-08-07 at the Wayback Machine "House of Low Culture Artist Page". Neurot ... House of Low Culture is a musical project which is fronted by Aaron Turner, and includes other members of the band Isis. The ...
In 2008, Uygun was awarded a Career Award by NIDDK at NIH "Computer-Aided Development of a Liver Organ Culture System". In 2009 ... Uygun's technique developed functional, transplantable rat liver grafts. The study was published in Nature Medicine. Uygun ... utilize untransplantable organs as cell sources for cell transplantation or bioartificial organ substitutes, such as ... A major research objective of Uygun is to enhance utilization of discarded donor organs in order to either increase the supply ...
More recent adaptations have further enabled the use of the technique for studies of specimens on the adult mouse organ scale, ... and longitudinal assessments of organ cultures. Fluorescence optical projection tomography visualises the distribution of dyes ... the emission mode of OPT can yield 3D genetic expression images of the mouse organ. The technique has already contributed to a ... The OPT technique is sometimes referred to as Optical Computed Tomography (optical-CT) and Optical Emission Computed Tomography ...
... embryo culture techniques MeSH E05.200.249.484 - organ culture techniques MeSH E05.200.249.617 - tissue culture techniques MeSH ... cell culture techniques MeSH E05.200.249.374 - coculture techniques MeSH E05.200.249.437 - diffusion chambers, culture MeSH ... fluorescent antibody technique MeSH E05.200.750.551.512.240.300 - fluorescent antibody technique, direct MeSH E05.200.750.551. ... patch-clamp techniques MeSH E05.200.750.132 - autoradiography MeSH E05.200.750.210 - bone demineralization technique MeSH ...
For example, small organisms, condensed organelles, and cultured cells, tissues, or organs can be measured with little ... Sun, T.; Xu, Y.; Li, P.; Yu, S.G.; Yin, L.P. (2007). "Non-invasive scanning ion-selective electrode technique and its ... Plenty of other large samples can be measured as well, such as organs or whole small organisms like zebrafish. The sample must ... Fick's laws of diffusion Nernst equation Bioelectronics Patch clamp Diffusion Scanning vibrating electrode technique ...
In vitro culture makes this system easy to manipulate and facilitates their monitoring. While organs are difficult to culture ... These advancements in PSC fate direction, coupled with the advancements in 3D culturing techniques allowed for the creation of ... Organoids have also been created using adult stem cells extracted from the target organ, and cultured in 3D media. A multitude ... Lancaster and Knoblich define an organoid as a collection of organ-specific cell types that develops from stem cells or organ ...
Sustainable and industrial production of guaianolides based on organ tissue culture, 2017-02-02, retrieved 2018-07-21. ... A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues". Journal of Visualized Experiments (136 ...
The cooking technique is rather complicated and takes a long time, making it a delicious and durable food. In 2011, an online ... But also using modern musical instruments such as organ, piano, guitar and drums. Many contemporary Minang songs also follow ... Minangkabau culture is the culture of the Minangkabau ethnic group in Indonesia, part of the Indonesian culture. This culture ... This culture has egalitarian, democratic, and synthetic characteristics. Minangkabau culture adheres to a matrilineal system in ...
The use of 3D cell cultures in microfluidic devices has led to a field of study called organ-on-a-chip. The first report of ... and physics to develop devices and techniques for culturing, maintaining, analyzing, and experimenting with cells at the ... Mixed co-culture is the simplest co-culture method, where two types of cells are in direct contact within a single culture ... co-culture, and segregated (or indirect) co-culture. The use of conditioned media, where the cultured medium of one cell type ( ...
They are stored using a hypothermic storage method or an organ culture method in a tissue culture medium. Corneal buttons ... Since 1905, various techniques and procedures have been developed to increase the effect of the transplantation and success ... The organ culture method, first introduced in 1976, is however quite complicated. The corneal button is stored in an incubator ... Penetrating keratoplasty using 37 C organ cultured cornea. Transactions of the American Academy of Ophthalmology and ...
The agency also included the publishing of books related to folk art and popular culture, with topics such as s purpura, a dye ... which focuses on developing contemporary products using traditional techniques. It also works to ensure that this handcraft ... made from a sea snail, organ grinders and charro music from northern Mexico. She also developed a project to preserve weaving ... She founded the Asociacíon Mexicana de Arte y Cultura Popular (AMACUP) or Mexican Association of Popular Art and Culture in ...
"Section 30.2Each Organ Has a Unique Metabolic Profile". Biochemistry 5th Edition. W H Freeman. Retrieved 29 April 2012.. ... The issue of healthy eating has long been an important concern to individuals and cultures. Among other practices, fasting, ... dieting, and vegetarianism are all techniques employed by individuals and encouraged by societies to increase longevity and ... Eating positions vary according to the different regions of the world, as culture influences the way people eat their meals. ...
Traditional medicine refers to the pre-scientific practices of a certain culture, in contrast to what is typically practiced in ... Use of standard-of-care treatment in addition to an alternative technique being tested may produce confounded or difficult-to- ... and an incorrect model of the anatomy and physiology of internal organs.[8][59][60][61][62][63] ... Edzard Ernst characterized the evidence for many alternative techniques as weak, nonexistent, or negative[108] and in 2011 ...
World Health Organ. 56 (2): 271-93. 1978. PMC 2395567. PMID 307456. Archived from the original (PDF) on 8 August 2014. ... Society and culture. See also: Cultural effects of the Ebola crisis. Weaponisation. Ebolavirus is classified as a biosafety ... Education of caregivers in these techniques, and providing such barrier-separation supplies has been a priority of Doctors ... Hewlett, Barry; Hewlett, Bonnie (2007). Ebola, Culture and Politics: The Anthropology of an Emerging Disease. Cengage Learning ...
... humans consumed animal organ meats, including the livers, kidneys, and brains. Upper Paleolithic cultures appear to ... that was more elaborate than previous Acheulean techniques.[4] This technique increased efficiency by allowing the creation of ... Middle Paleolithic stone tool manufacturing spawned a tool making technique known as the prepared-core technique, ... "Intro to archeology The First People and Culture". Introduction to archeology. Retrieved 2008-03-20.. ...
Oral swabs are taken if culture is required. Some recommend that swabs be taken from 3 different oral sites. Oral rinse ... Oral rinse technique can distinguish between commensal candidal carriage and candidiasis. If candidal leukoplakia is suspected ... organ transplantation and use of indwelling catheters). Oral candidiasis has been recognized throughout recorded history. The ... With more sensitive detection techniques, this figure is reported to rise to 90%. This candidal carrier state is not considered ...
Newer techniques using ex-vivo expansion of cord blood units or the use of two cord blood units from different donors allow ... Unlike other organs, bone marrow cells can be frozen (cryopreserved) for prolonged periods without damaging too many cells. ... during which neither showed traces of HIV in their blood plasma and purified CD4 T cells using a sensitive culture method (less ... The technique is referred to as a bone marrow harvest and is performed under general anesthesia. ...
Comics As a Nexus of Cultures: Essays on the Interplay of Media, Disciplines. McFarland. p. 203. ISBN 978-0-7864-3987-4.. ... Octopus reproductive organs mature due to the hormonal influence of the optic gland but result in the inactivation of their ... Kassim, I.; Phee, L.; Ng, W. S.; Gong, F.; Dario, P.; Mosse, C. A. (2006). "Locomotion techniques for robotic colonoscopy". ... The ink is thought to reduce the efficiency of olfactory organs, which would aid evasion from predators that employ smell for ...
World Health Organ. 91 (4): 277-282. doi:10.2471/BLT.12.110015. PMC 3629451. PMID 23599551.. ... Folk medicine consists of the healing practices and ideas of body physiology and health preservation known to some in a culture ... Since knowledge, techniques, and medical technology continue to evolve at a rapid rate, many regulatory authorities require ... "Assessing patients effectively: Here's how to do the basic four techniques". Nursing2014. 8 (2): 6. 2006.. ...
The History and Culture of Iroquois Diplomacy: An Interdisciplinary Guide to the Treaties of the Six Nations and Their League. ... They developed sophisticated techniques of hunting and managing their resources.. According to Dutch settler Isaac de Rasieres ... and use compound containing root bark as a tonic for the female generative organs. [36] ... Carman, Alan, E. Footprints in Time: A History and Ethnology of The Lenape-Delaware Indian Culture. Trafford Publishing, 2013. ...
Organ jarak jauh: Sebuah organ jarak jauh hidraulik yang dapat didengari dari enam puluh batu jauh telah dijelaskan dalam ... David J Roxburgh (2000), Muqarnas: An Annual on the Visual Culture of the Islamic World, p. 21, Brill Publishers, ISBN ... G. R. Tibbetts (1973), "Comparisons between Arab and Chinese Navigational Techniques", Bulletin of the School of Oriental and ... Sarton, George (1932), "Reviewed work(s): The Organ of the Ancients by Henry George Farmer", Isis, 17 (1): 278-282 [281]. ...
"Guide to Research Techniques in Neuroscience" (2009). *^ Squire, L. et al. "Fundamental Neuroscience, 4th edition" (2012). ... "Floating Man - The Art and Popular Culture Encyclopedia". www.artandpopularculture.com. Archived from the original on 26 April ... Al-Nafis concluded that "the soul is related primarily neither to the spirit nor to any organ, but rather to the entire matter ... Yu, Jose Vidamor B. (2000). Inculturation of Filipino-Chinese Culture Mentality. Interreligious and Intercultural ...
"The Monthly: Australian politics, societies, and cultures.. *. Tsai, Wen-Chu. "Whales and trash-bags". Taipei Times. Retrieved ... Some whales, such as the bowhead whale, possess a vomeronasal organ, which does mean that they can "sniff out" krill.[55] ... They use a suction technique, aided by a pair of grooves on the underside of their head, not unlike the throat pleats on the ... The presence of the Jacobson's organ indicates that whales can smell food once inside their mouth, which might be similar to ...
... by means of penile inversion or the sigmoid colon neovagina technique; or, more recently, non-penile inversion techniques that ... Society and culture[edit]. The Iranian government's response to homosexuality is to endorse, and fully pay for, sex ... She started with the removal of her original sex organs, the operation supervised by Dr. Magnus Hirschfeld. Lili went on to ... Del Priore, G.; Stega, J.; Sieunarine, K.; Ungar, L.; Smith, J. R. (2007). "Human Uterus Retrieval From a Multi-Organ Donor". ...
The main organs involved in urination are the urinary bladder and the urethra. The smooth muscle of the bladder, known as the ... The technique can help children to urinate discreetly inside cars and in other places without being seen by others.[30] ... In Western culture, the standing position is regarded by some as superior and more masculine than the sitting or squatting ... A common technique used in many undeveloped nations involves holding the child by the backs of the thighs, above the ground, ...
The examples and perspective in this article deal primarily with Western culture and do not represent a worldwide view of the ... This technique requires the integration of the same doctor in two different capacities: surgeon as well as pathologist. In case ... Immunodermatology testing is essential for the correct diagnosis and treatment of many diseases affecting epithelial organs ... The dermatologic subspecialty called Mohs surgery focuses on the excision of skin cancers using a tissue-sparing technique that ...
It was to take advantage of the aura of workplace power that the word became used by Lenin for various political organs. Indeed ... Sterba 1982, p. 35: "This book [Character Analysis] serves even today as an excellent introduction to psychoanalytic technique ... where his classes on psychoanalytic technique, later presented in a book called Character Analysis, were crucial to his whole ...
Other techniques used by blind people to assist them in daily activities include: *Adaptations of coins and banknotes so that ... Society and cultureEdit. See also: List of blind people and Blind musicians ... Uveitis: is a group of 30 intraocular inflammatory diseases[44] caused by infections, systemic diseases, organ-specific ... However, techniques for cane travel can vary depending on the user and/or the situation. Some visually impaired persons do not ...
Based on the assumed superiority of French culture, in practice the assimilation policy meant the extension of French language ... Bureau Technique Permanent du Recensement, 2002. ... Organs. *Executive Council. *Permanent Representatives' ... These programmes correspond to eight priority sectors for 2012-2015, namely: health, raw materials, agriculture, culture, ... others thought the problem of the division between the tribal culture and modernity would continue.[41] ...
... reporting on the re-implantation of culture-expanded mesenchymal stem cells using autologous platelet lysate technique". ... For years, the concept of harvesting stem cells and re-implanting them into one's own body to regenerate organs and tissues has ... The AMIC technique was developed to improve some of the shortfalls of microfracture surgery such as variable repair cartilage ... "Maci" or autologous cultured chondrocytes on porcine collagen membrane, is a treatment to correct cartilage defects in the knee ...
Salavetz, Judith; Drate, Spencer (2010), Creating Comics! 47 Master Artists Reveal the Techniques and Inspiration Behind Their ... "The Organ Donor's March". They raised $17,000 USD on Kickstarter from over 400 backers in April 2011.[54] ... Her impressions of the artistry and culture of the Ottoman Empire in the Near East would come to influence her style and work.[ ... Crabapple uses a crosshatch style of illustration based on Arthur L. Guptill's art technique found in Rendering in Pen and Ink ...
The techniques by which cells are studied have evolved. Advancement in microscopic techniques and technology such as ... then organs and ultimately systems.[12] The G phases along with the S phase - DNA replication, damage and repair - are ... Modern day cell biology research looks at different ways to culture and manipulate cells outside of a living body to further ...
Modern techniques[edit]. Many new techniques have been developed [28] and are used in order to more accurately gather evidence ... and appearance to the organ pipe mud dauber wasp and its relatives. ... of Mégnin's work that the studies of forensic science and entomology became an established part of Western popular culture, ... There are many techniques currently being developed to differentiate between the various species of forensically important ...
Other surgical techniques include partial ileal bypass surgery, in which part of the small bowel is bypassed to decrease the ... but at the risk of complications from any solid organ transplant (such as rejection, infections, or side-effects of the ... "Familial hypercholesterolemia: defective binding of lipoproteins to cultured fibroblasts associated with impaired regulation ...
Tissue culture[28][9]Edit. Plant cells are taken from various parts of the plant and are cultured and nurtured in a sterilized ... Over the years, horticulturalists have developed asexual propagation techniques that use vegetative plant parts, to replicate ... since specialized organs of vegetative reproduction, like seeds of annuals, serve to survive seasonally harsh conditions. A ... Bonga, J. M. (1982). Tissue Culture in Forestry. Forestry Sciences. Springer, Dordrecht. pp. 387-412. doi:10.1007/978-94-017- ...
Harmke Kamminga (1995). The Science and Culture of Nutrition, 1840-1940. Rodopi. p. 31. ISBN 978-90-5183-818-3. Moleschott's ... Melanie Klein (1882-1960): Austrian-born British psychoanalyst who devised novel therapeutic techniques for children that ... the Golgi tendon organ and the Golgi tendon reflex. He is recognized as the greatest neuroscientist and biologist of his time.[ ... Donald De Marco; Donald DeMarco; Benjamin Wiker (2004). Architects of the Culture of Death. Ignatius Press. p. 223. ISBN ...
Society and culture[edit]. Marketing[edit]. Vaginal steaming is marketed with pseudoscientific notions of "balancing" female ... Vaginal steaming is described in spas as an ancient Korean treatment for reproductive organ ailments and is claimed to have ... In a paper for Culture, Health & Sexuality, Vandenburg and Braun argue that the rhetoric of vaginal steaming mirrors sexist ... Bowen technique. *Breathwork. *Cancer treatments *Greek cancer cure. *Charcoal cleanse. *Chiropractic *Chiropractic treatment ...
A high rate above the survival threshold may kill cancer cells with a high apoptotic threshold.[86][87] This technique can be ... By inhibiting autophagy genes in these tumors cells, regression of the tumor and extended survival of the organs affected by ... as well as that of nutrient starved cultured cells and tissues.[59][60] Mutant yeast cells that have a reduced autophagic ... Mechanical injury to cartilage explants in culture also reduced autophagy proteins.[77] Autophagy is constantly activated in ...
organ. A collection of tissues joined in a structural unit to serve a common function.. organism. A contiguous living system.. ... A technique used in molecular biology to amplify a single copy or a few copies of a segment of DNA across several orders of ... A genetic variant, subtype, or culture identified as a distinct taxonomic subdivision within a species. The term is most ... Rather, semi-solid mesodermal tissues between the gut and body wall hold the animal's organs in place. Contrast coelomate and ...
His technique was used by Santiago Ramón y Cajal and led to the formation of the neuron doctrine, the hypothesis that the ... In many species - including all vertebrates - the nervous system is the most complex organ system in the body, with most of the ... "Phrenology in the science and culture of the 19th century". Neurosurgery. 37 (4): 790-805. doi:10.1227/00006123-199510000- ... The emergence of powerful new measurement techniques such as neuroimaging (e.g., fMRI, PET, SPECT), electrophysiology, and ...
"Djibouti - Culture Overview". Expedition Earth. Archived from the original on 27 February 2004. Retrieved 28 September 2005.. ... In recent years, Djibouti has improved its training techniques, military command and information structures and has taken steps ... "The Culture Trip. Archived from the original on 6 March 2019. Retrieved 4 March 2019.. ... Abdullahi, Mohamed Diriye (2001) Culture and Customs of Somalia. Greenwood Press. pp. 170-172. ISBN 9780313313332 ...
"Organ Culture Techniques" by people in Harvard Catalyst Profiles by year, and whether "Organ Culture Techniques" was a major or ... A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated ... "Organ Culture Techniques" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... Organ Culture Techniques*Organ Culture Techniques. *Culture Technique, Organ. *Culture Techniques, Organ ...
Organ Culture. Vessel segments were transported to the laboratory in warm (25°C to 30°C), sterile, 20 mmol/L HEPES-buffered ... Surgical Technique. The study was conducted in 28 Land Race pigs 3 to 4 months old weighing 25 to 30 kg, all of which received ... The remaining tissue segments were subjected to organ culture by using the method of Francis et al8 with the following slight ... Segments were subjected to organ culture in serum-free medium for 2 days as described in Methods. Conditioned media were ...
Organ culture techniques‎ (42 F). *. ► Organ printing‎ (5 F). P. *. ► Pharmaceutical companies‎ (21 C, 30 F) ...
Organ culture techniques‎ (42 Ն). *. ► Organ printing‎ (5 Ն). *. ► Organ-on-a-chip technology‎ (11 Ն) ...
Organ Culture Techniques * Rats * Rats, Wistar * Receptors, N-Methyl-D-Aspartate / physiology ... using the minimal stimulation technique in slices in whole cell recorded mossy cells retrogradely prelabeled with the ...
Organ Culture Techniques * Thorax / abnormalities * Transfection * Wilms Tumor / genetics Substances * Alkaline Phosphatase ...
Tissue and organ culture techniques of producing seed yams. Plant Tissue Culture (PTC) is the growth of small plant parts in a ... While tissue and organ culture techniques are the most rapid methods of multiplying disease free propagules, their limitations ... Tissue and organ culture to produce seed yam: 1. Field plants; 2. Single nodes from the field for in vitro culture; 3. Batch of ... Plant Cell Tissue and Organ Culture, 81(3), 255-264.CrossRefGoogle Scholar ...
Three-dimensional Organotypic Cultures of Vestibular and Auditory Sensory Organs, Perfusable Vascular Network with a Tissue ... A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering, Isolation and Culture of ... culture techniques include Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow, ... Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model, Endothelialized Microfluidics for ...
Purchase Cells and Tissues in Culture Methods, Biology and Physiology - 1st Edition. Print Book & E-Book. ISBN 9781483231464, ... Techniques of Organ, Tissue and Free Cell Culture References 9. The Nutrition and Metabolism of Plant Tissue and Organ Cultures ... and organ cultures in virus research.. The book elaborates on antibody production in tissue culture and tissue culture in ... Virus Studies in Organ Cultures References 5. Antibody Production in Tissue Culture. I. Introduction II. Early Attempts at in ...
Posterior Lamellar Keratoplasty Using Descemetorhexis and Organ-Cultured Donor Corneal Tissue (Melles Technique). Nieuwendaal, ... Spectral Optical Coherence Tomography: A Novel Technique for Cornea Imaging. Kaluzny, Bartlomiej J; Kaluzny, Jakub J; ... Deep Anterior Lamellar Keratoplasty Using the Manual Dissection Technique of Melles: A Histopathologic Correlation. Saw, ...
Development of a refined tenocyte differentiation culture technique for tendon tissue engineering (Cells Tissues Organs (2013) ... Development of a refined tenocyte differentiation culture technique for tendon tissue engineering (Cells Tissues Organs (2013) ...
Organ Culture Techniques. Oxidative Stress / physiology. Potassium Channel Blockers / pharmacology. Potassium Channels / drug ...
Organ Culture Techniques. A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional ... Histological Techniques. Methods of preparing tissue for examination and study of the origin, structure, function, or pathology ... cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture ... Evaluation of the ScalpelCric Set and Comparison With Two Further Techniques of Cricothyrotomy ...
Organ Culture Techniques. Ovarian Follicle / drug effects, metabolism*. Ovary / anatomy & histology, drug effects, metabolism* ... Treatment of 2-d-old rat ovaries in organ culture with NGF increased FSH receptor (FSHR) mRNA within 8 h of exposure. This ...
Spheroid culture is one way of applying three-dimensional culture. This report describes the two-dimensional culture and ... spheroid culture and the morphological comparison will be performed between two-dimensional culture and spheroid culture. ... Traditionally, two-dimensional culture has been applied for stem cell research. However, more recently, a three-dimensional ... organ culture techniques. *stem cells. chapter and author info. Authors. *. Sae Kyung Min. *Department of Periodontics, College ...
... tissue and organ culture : fundamental methods. [O L Gamborg; Gregory C Phillips;] -- A manual providing all relevant protocols ... Techniques de culture dorganes--Manuel de laboratoire. a schema:Intangible ;. schema:name "Techniques de culture dorganes-- ... Cultures et milieux de culture--Manuels de laboratoire. a schema:Intangible ;. schema:name "Cellules végétales--Cultures et ... culture_manuels_de_laboratoire> ; # Cellules végétales--Cultures et milieux de culture--Manuels de laboratoire. schema:about < ...
Tissue morphology was studied using routine histologic, histochemical and immunohistochemical techniques. Cell function was ... TGF-beta3 maintains the phenotype of disc cells in organ culture. It exerts this effect, in part, by elevating the levels of ... To develop an in vitro organ culture of rat intervertebral disc and evaluate effects of TGF-beta3 on disc cell function. ... Rat lumbar intervertebral discs were maintained in organ culture in media supplemented with TGF-beta3 or TGF-beta1 for 1 week. ...
Screening techniques were developed to obtain disease (Fusarium wilt, black Sigatoka) and nematode resistant plants, while ... entitled Cellular biology and biotechnology including mutation techniques for creation of new useful banana genotypes . In 1996 ... Plant Cell Tissue Organ Culture 66 (2001) 189-197.. [33] SAS INSTITUTE INC. 1996. SAS/STAT Users Guide release 6 (12th ed.), ... In vitro culture, radiosensitivity and mutation induction. In vitro cultures of the cultivar Lakatan were provided by the IPB ...
Using Cell and Organ Culture Models to Analyze Responses of Bone Cells to Mechanical Stimulation ... Focusing mainly on in vitro methods, this volume gives techniques for isolation, culture and functional analysis of all bone ... Primary Human Osteoblast Cultures Jane P. Dillon, Victoria J. Waring-Green, Adam M. Taylor, Peter J. M. Wilson, Mark Birch, ... Bone Research biochemical analysis bone mass bone strength in vivo loading techniques inherited bone disorders mesenchymal stem ...
T. Ochiya and M. Terada, Antisense Approaches to in vitro Organ Culture. E.S. Raveche, G.A. Parker, B. Peng, M. He, S. Gould- ... S.G. Volsen, R.C. Lambert, Y. Maulet, M.D. Waard, S. Gillard, P.J. Craig, and A. Feltz, The Application of Antisense Techniques ... D.L. Mattson, Use of Antisense Techniques in the Rate Renal Medulla. ... is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or ...
A high-throughput template for optimizing Drosophila organ culture with response-surface methods ... A high-throughput template for optimizing Drosophila organ culture with response-surface methods ... A high-throughput template for optimizing Drosophila organ culture with response-surface methods ... A high-throughput template for optimizing Drosophila organ culture with response-surface methods ...
Development of an air-interface in vitro organ culture model (IVOC) of bovine respiratory epithelium ... Fresh bovine respiratory tissues obtained from healthy cattle will be used to establish nasal and tracheal organ culture ... Development of an air-interface in vitro organ culture model (IVOC) of bovine respiratory epithelium ... Critical assessment of an in vitro bovine respiratory organ culture system: a model of bovine herpesvirus-1 infection. J Virol ...
This Review provides an overview of bioengineering technologies that can be harnessed to facilitate the culture, self- ... This technology generally relies on traditional three-dimensional culture techniques that exploit cell-autonomous self- ... Trowell, O. A. A modified technique for organ culture in vitro. Exp. Cell Res. 6, 246-248 (1954). ... This technology generally relies on traditional three-dimensional culture techniques that exploit cell-autonomous self- ...
... the global cell and tissue culture supplies market is estimated to reach $36,744 ... Complexity of cell culture techniques and moral concern over mismanagement of animals may hamper the market growth. Furthermore ... Global Cell and Tissue Culture Supplies Market Study: Growing Demand for Artificial Organs Noticed by Variant Market Research. ... Increasing investments on R&D for cell-based research and tissue engineering to drive the global cell and tissue culture ...
Gene delivery by viral vectors in primary cultures of lacrimal gland tissue. - Eyal Banin, Alexey Obolensky, Elena Piontek, ... Tissue was maintained under primary organ culture conditions using the "raft" technique. The ability of three different viral ... Gene delivery by viral vectors in primary cultures of lacrimal gland tissue.. Abstract. PURPOSE: To test the feasibility of ... After 7 days in culture, successful gene delivery occurred in 77% of vaccinia, 41% of adenovirus, and only 13% of herpesvirus ...
... labeled with a variety of fluorophores using both traditional staining methods as well as immunofluorescence techniques. ... This initial organ culture was used to establish several distinct astroglial cell lines. The C8-B4 clone was created in 1996, ... The term tissue culture arose because most of the early cells were derived from primary tissue explants, a technique that ... Serious attempts at the culture of whole tissues and isolated cells were first undertaken in the early 1900s as a technique for ...
German Researchers Develop Novel 3D Cell Culture Technique to Study Gliomas.... Kidney Organoid Method Might Just Stir the ... "Our organs are 3D in nature. For many years, electrophysiology was done using just cells cultured on a 2D tissue culture dish. ... Home Cell Culture 3D Cell Culture Organ-on-e-chip Biosensor Shrink Wraps Heart Cells ... "For decades, electrophysiology was done using cells and cultures on two-dimensional surfaces, such as culture dishes," ...
We describe a three-tissue organ-on-a-chip system, comprised of liver, heart, and lung, and highlight examples of inter-organ ... Few engineered model systems, including the growing variety of organoid and organ-on-a-chip platforms, have so far reflected ... drug candidate to successful use is a lack of model systems that accurately recapitulate normal tissue function of human organs ... A four-organ-chip for interconnected long-term co-culture of human intestine, liver, skin and kidney equivalents. Lab Chip 15, ...
A Comparison of Standard Organ Culture and Standard Transplant Techniques in the Fusion of the Palat. Original source: ; ; ; ; ... It is split into several sections describing different techniques for finding work. You can dip in and out of the different ...
Plant Cell Tissue and Organ Culture. doi:10.1007/s11240-015-0899-8. ... Improvement of cryopreservation technique for long-term storage of shoot tips of Ipomoea batatas. Society for In Vitro Biology ... Improvement of cryopreservation technique for long-term storage of shoot tips of Ipomoea batatas. American Society of ... IMPLEMENTATION OF GARLIC CRYOPRESERVATION TECHNIQUES IN THE NATIONAL PLANT GERMPLASM SYSTEM - Ellis, D.D., D. Skogerboe, C. ...
  • It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (harvard.edu)
  • Ex Vivo Oculomotor Slice Culture from Embryonic GFP-Expressing Mice for Time-Lapse Imaging of Oculomotor Nerve Outgrowth. (harvard.edu)
  • We tested the effects of activated NRas(Q61K) on the proliferation, motility, and invasiveness of melanoblasts and melanocytes in the developing mouse and ex vivo explant culture as well as in a melanoma transplant model. (nih.gov)
  • Organotypic cultures or ex vivo models maintain the architecture of the tissues closer to the in vivo setting, overtaking some of the limitations of the in vitro studies [4]. (thefreelibrary.com)
  • Several biological models have been used to illustrate the technique in cell cultures and organs in vivo. (unboundmedicine.com)
  • This interdisciplinary research project aims to develop barrier-on-chip models where several different cell types can be cultured together in an in vivo- like environment. (uu.se)
  • In this study, using the combination of MRT techniques and a unique ex vivo testes organ culture, we show, for the first time, the MRT-mediated TSE for the preservation of spermatogenesis. (qub.ac.uk)
  • These are being investigated in a variety of ways, all of which use animals, either in vivo or by organ culture of kidneys isolated from embryos. (nc3rs.org.uk)
  • 3D culturing has become popular in the field of drug-screening and toxicity, as the cells respond to chemicals in a more ' in vivo way' than 2D cultures. (labx.com)
  • Balls of interacting cells form 'organoids' or 'organ spheroids' that replicate tissues in vivo . (labx.com)
  • At CLS, she led the development of various new products including advanced extracellular matrices/surface coatings and laboratory devices for culture of mammalian primary and stem cells. (corning.com)
  • It is not my purpose to review the field of experimental embryology that deals with tissue culture of mammalian embryonic organs ( Wessells 1967). (springer.com)
  • Three-dimensional organotypic culture: experimental models of mammalian biology and disease. (nih.gov)
  • Mammalian organs are challenging to study as they are fairly inaccessible to experimental manipulation and optical observation. (nih.gov)
  • Recent advances in three-dimensional (3D) culture techniques, coupled with the ability to independently manipulate genetic and microenvironmental factors, have enabled the real-time study of mammalian tissues. (nih.gov)
  • This method was found to be effective for culturing mammalian hepatocytes. (labx.com)
  • This Center studies how cell behavior guides normal development, including the creation and maintenance of tissues and organs. (nih.gov)
  • The primary aim is to understand how tissues are organized at all structural levels, including the molecular and macromolecular, the entire cell and intercellular substances, and the tissues and organs. (thefreedictionary.com)
  • Based on previous publications on storage of cultured epithelial cells [ 20 - 23 ], we hypothesize that differences in storage temperature between 4°C and 37°C affect the viability, morphology, and phenotype of cultured RPE cells. (hindawi.com)
  • In addition, primary epithelial cells from the salivary gland, lung, kidney and pancreas, as well as endothelial cells, have been used in two and one-half-dimensional (2.5D) or 3D culture. (nih.gov)
  • Primary neurons are co-cultured with epithelial cells to ask how signaling pathways communicate. (bu.edu)
  • Embryonic organ culture is an easier alternative to normal organ culture derived from adult animals. (wikipedia.org)
  • The following are four techniques employed for embryonic organ culture. (wikipedia.org)
  • Embryonic organs generally grow well on agar, but adult organ culture will not survive on this medium. (wikipedia.org)
  • A number of embryonic organs and tissues are known to undergo morphogenesis and attain functional maturation upon explanation in vitro, and their capabilities have been exploited to a variable degree in investigations on developmental mechanisms and the processes of cell differentiation ( Saxén and Kohonen 1969). (springer.com)
  • for example, explants of metanephric kidney that have been isolated from the embryonic urogenital ridge will undergo vigorous branching morphogenesis in three-dimensional (3D) culture. (nih.gov)
  • for example, embryonic stem (ES) cells that are cultured in the presence of Matrigel and differentiation factors will aggregate and self-organize into optic cup-like structures. (nih.gov)
  • Based on our existing systems, we will produce these cell lines both by conditional immortalization of cells from embryonic kidneys and by sorting and culture of ES cell derivatives. (nc3rs.org.uk)
  • siRNA-mediated RNA interference in embryonic kidney organ culture. (nc3rs.org.uk)
  • 2010). A novel, low-volume method for organ culture of embryonic kidneys that allows development of cortico-medullary anatomical organization. (nc3rs.org.uk)
  • Weiss P, T. A. Reconstitution of complete organs from single-cell suspensions of chick embryos in advanced stages of differentiation. (nature.com)
  • Primary keratinocytes from the skin and oesophagus have been cultured on cell culture inserts to organize into stratified epithelium. (nih.gov)
  • In corneal transplantation, the development of storage techniques has simplified surgery logistics, enabled quality control and tissue transportation, and provided worldwide tissue availability. (hindawi.com)
  • Eliminating fetal calf serum (FCS) from corneal organ culture (OC) media has long been a challenge. (arvojournals.org)
  • Organ culture (OC) at +31°C to 37°C is the corneal storage technique of choice in Europe, used exclusively by the 21 French eye banks and most other European banks. (arvojournals.org)
  • Standard CXL (S-CXL) technique involves the removal of corneal epithelium to allow penetration of riboflavin. (hindawi.com)
  • Finally, recent advantages in preservation of cultured corneal/limbal epithelia may increase the availability of this tissue and therefore increase treatment of limbal stem cell deficiency. (uio.no)
  • The term tissue culture arose because most of the early cells were derived from primary tissue explants, a technique that dominated the field for over 50 years. (fsu.edu)
  • In a combination of raft and clot techniques, the explants are first placed on a suitable raft, which is then kept on a plasma clot. (wikipedia.org)
  • In vitro studies, using cultured jejunal explants incubated with either [ 14 C]oleic acid or [ 35 S]methionine, revealed their higher efficiency in de novo TG synthesis, apo B-48 biogenesis, and TG-rich lipoprotein assembly. (diabetesjournals.org)
  • In fetal gut organ cultures epithelial expression of E-cadherin was lower when local T cells were activated with mitogens, compared with control explants. (elsevier.com)
  • We show that hindbrain explants prepared at the somitogenesis stage undergo normal morphogenesis for at least 14h in culture. (zfin.org)
  • Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture. (nature.com)
  • A tympanal organ (or tympanic organ) is a hearing organ in insects, consisting of a membrane (tympanum) stretched across a frame backed by an air sac and associated sensory neurons. (wikipedia.org)
  • Isolation, Culture and Differentiation of Progenitor Cells from the Central Nervous System," from Larysa Pevny's laboratory at the University of North Carolina ( http://www.unc.edu/~pevny/ ), describes the neurosphere assay, which allows one to identify neural progenitor cells as well as to determine both their self-renewal capacity and their ability to generate three primary cell types of the nervous system: neurons, astrocytes and oligodendrocytes. (bio-medicine.org)
  • Organ culture seems to offer several advantages, yet the use of commercial fetal calf serum gives concern regarding the possible transfer of unknown microbiological agents. (uio.no)
  • Human CD34 + CD38 − fetal liver cells, cultured overnight in a combination of stem cell factor and interleukin-7 (IL-7), could be transduced with 30% efficiency. (bloodjournal.org)
  • Fetal Organ Culture. (nc3rs.org.uk)
  • and frozen sections of cultured fetal gut in which a T cell mediated enteropathy had been induced. (elsevier.com)
  • and growth, differentiation and organogenesis in plant tissue and organ cultures. (elsevier.com)
  • The techniques presented range from experimental animal models, to animal and human placental organ and cell culture systems, to morphological, biochemical, and molecular strategies for assessing trophoblast/placental growth, differentiation and function. (google.com)
  • A non-radioactive silver staining technique for AFLP markers suitable for local laboratory conditions has been established. (fao.org)
  • To date, however, laboratory studies have been largely confined to cells grown in two dimensions in culture dishes. (genengnews.com)
  • Basic laboratory techniques are employed for plant and animal tissue, organ culture preparation and virus cultivation. (oxnardcollege.edu)
  • COLD SPRING HARBOR, N.Y. (Mon., Nov. 3, 2008) Understanding the function of organs like the brain, kidney, and reproductive tissues requires experimental systems that allow for the study and manipulation of developing cells and tissues in the laboratory. (bio-medicine.org)
  • Blanche Capel's laboratory at Duke University (http://www.cellbio.duke.edu/faculty/capel/index.html) provides "Preparing Recombinant Gonad Organ Cultures. (bio-medicine.org)
  • A collection of cutting-edge laboratory techniques for the study of trophoblast and placental biology. (google.com)
  • This volume describes up-to-date techniques for improved production of secondary metabolites of economic interest using field and laboratory methods. (springer.com)
  • I also encountered the name of Giuseppe Levi, who had visited the country a few years before and helped set up the first tissue culture laboratory in Uruguay. (asmblog.org)
  • This is the one complete diseases laboratory in the Netherlands for cultured fish for human consumption, ornamental and wild fish and shellfish. (wur.nl)
  • It was the first fish diseases laboratory in Europe accredited for quality standards ISO 9001 and 17025 (EN 45001). (wur.nl)
  • Among the modern methods of seed yam production, only the minisett technique, which uses 25-100 g tuber pieces, is currently used at farmer level, although on a limited scale. (springer.com)
  • While tissue and organ culture techniques are the most rapid methods of multiplying disease free propagules, their limitations include high costs, need for skilled personnel and specialized equipment. (springer.com)
  • To build and sustain a viable seed yam production system, a multiplication scheme is required that combines two or more methods including tissue culture for cleaning the seed stock. (springer.com)
  • Cells and Tissues in Culture: Methods, Biology, and Physiology, Volume 3 focuses on the applications of the methods of tissue culture to various fields of investigation, including virology, immunology, and preventive medicine. (elsevier.com)
  • Discussions focus on early attempts at in vitro studies, tissue culture in the study of pharmacologically active agents, and methods of assessment of drug activity. (elsevier.com)
  • This fluorescence image gallery explores over 30 of the most common cell lines, labeled with a variety of fluorophores using both traditional staining methods as well as immunofluorescence techniques. (fsu.edu)
  • To test the bactericidal activity of standard organ culture medium, and to compare the sensitivity and rapidity of blood culture bottles with conventional microbiological methods for detection of bacteria and fungi inoculated in a standard cornea organ culture medium. (bmj.com)
  • Organ culture is a development from tissue culture methods of research, the organ culture is able to accurately model functions of an organ in various states and conditions by the use of the actual in vitro organ itself. (wikipedia.org)
  • With the advancement of RPE cell replacement therapy, and with 20-25 million known sufferers from AMD worldwide [ 17 ], a great need for improved storage methods for cultured RPE is likely to emerge. (hindawi.com)
  • Biotechnology of Plant Secondary Metabolism: Methods and Protocols explores different secondary metabolite classes, whole-plant and cell/organ culture systems, and environmental and genetic transformation-based modulation of biochemical pathways. (springer.com)
  • TGF-beta3 maintains the phenotype of disc cells in organ culture. (nih.gov)
  • This study investigates the effect of different storage temperatures on the viability, morphology, and phenotype of cultured RPE. (hindawi.com)
  • This work is revealing that the control of body and organ size does not reside in any specific cellular or molecular mechanism but that it is a systems property in which cellular, physiological and environmental signals all contribute in inextricable ways to produce the final phenotype. (duke.edu)
  • This will include elements such as system priming and cell seeding, the development of optimised and automated cell culture protocols, evaluation of barrier tightness and characterisation of genotype and phenotype of the cultured cells and relate this to their culture environment. (uu.se)
  • It is meritorious for the candidate to show documentation on culture optimisation, immunofluorescent staining, genotypic characterisation, phenotype evaluation and studies of cell growth and proliferation, especially focused on blood-brain barrier specific cells. (uu.se)
  • Highlights include strategies for the isolation and culture of trophoblast cells from primates, ruminants, and rodents, and precise guidance to the molecular and cellular analysis of the placental phenotype. (google.com)
  • Blood bottles detect more efficiently and more rapidly a wider range of bacteria and fungi than the conventional microbiological method and the visual inspection of organ culture media. (bmj.com)
  • Results were compared with those of microbiological culture, and the applicability of the technique in routine diagnosis was analyzed. (scielo.br)
  • Topics include cellular organization at the molecular level, fibrogenesis in tissue culture, effect of radiation on the growth of isolated cells, and irradiation of the selected parts of the cell. (elsevier.com)
  • The process of directed cellular movement is of critical importance to human health, as is observed when immune cells seek out infected tissues or metastatic cancer cells invade new organs. (nih.gov)
  • This research project demands a highly creative individual with strong analytical skills as well as practical skills regarding the handling of microfluidic systems and evaluation of cellular responses cultured in our various barrier-on-chip systems. (uu.se)
  • Biology courses at Oxnard College introduces the student to gross anatomy, histology of human organ systems, the basic principles of molecular and cellular biology, lower division biochemistry which offers the study of biochemical and cellular design and the regulation of that metabolism. (oxnardcollege.edu)
  • Stem cells can be used to generate organoids that model the architecture and cellular composition of a larger organ. (nih.gov)
  • The book elaborates on antibody production in tissue culture and tissue culture in pharmacology. (elsevier.com)
  • Traditionally, two-dimensional culture has been applied for stem cell research. (intechopen.com)
  • He has over 25 years experience in cell biology with specific interests in cell culture technology, tissue engineering and stem cell research. (smi-online.co.uk)
  • c Stem cell organoid cultures. (nih.gov)
  • Biological techniques: Kidney tissue grown from induced stem cells. (nc3rs.org.uk)
  • While cultures are predominantly neuronal, they also contain glia and stem progenitor cells. (zfin.org)
  • 2013). FAK-Src signalling is important to renal collecting duct morphogenesis: discovery using a hierarchical screening technique. (nc3rs.org.uk)
  • For virology organs of the fish are sampled, and in histopathology (microscopy of sections of affected tissue) pieces of tissue are put into a fixative. (wur.nl)
  • To address this challenge, we have developed an assortment of bioengineered tissue organoids and tissue constructs that are integrated in a closed circulatory perfusion system, facilitating inter-organ responses. (nature.com)
  • A team from the Centre for Trophoblast Research, which this year celebrates its tenth anniversary, was able to grow the organoids in culture from cells derived from endometrial tissue and maintain the organoids in culture for several months, faithfully reproducing the genetic signature of the endometrium - in other words, the pattern of activity of genes in the lining of the uterus. (eurekalert.org)
  • The technique also enables the researchers to grow organoids from endometrial cancer cells. (eurekalert.org)
  • The culture of organoids has permitted researchers access to a highly physiologically relevant system for studying human disease. (corning.com)
  • Human organoids reflect key structural and functional properties of organs such as kidney, lung, gut, brain and retina making them incredibly valuable, particularly in situations where there is an unmet need for an in vitro human model. (corning.com)
  • While their value is undeniable, culturing organoids can be quite challenging. (corning.com)
  • Culturing organoids is a likewise sensitive process and the specific requirements of the cells and the organoids needs to be evaluated. (corning.com)
  • Primary organs can be harvested and processed by mechanical disruption and enzymatic digestion into tissue fragments (known as tissue organoids). (nih.gov)
  • Organoid cultures have proven to be powerful tools for investigating the behaviour of other organ systems. (eurekalert.org)
  • Long-term, hormone-responsive organoid cultures of human endometrium in a chemically defined medium. (eurekalert.org)
  • b Tissue organoid cultures. (nih.gov)
  • Our experiments are conducted using a number of organ culture and cell models and use biochemical, cell and molecular techniques. (bu.edu)
  • It remains to be shown whether further improvements in storage methology also can bring other fields of regenerative medicine forward by improving the availability and safety of transplantation of cultured cells. (uio.no)
  • Media solidified with agar are also used for organ culture and these media consist of 7 parts 1% agar in BSS, 3 parts chick embryo extract and 3 parts of horse serum. (wikipedia.org)
  • The medium with agar provides the mechanical support for organ culture. (wikipedia.org)
  • For example, in the event of a bacteriological suspicion, specimens from the lesions and internal organs of the fish are inoculated onto agar. (wur.nl)
  • Inhalation exposure studies of mice were conducted to determine if multi-walled carbon nanotubes (MWCNT) distribute to the parietal pleura, respiratory musculature and systemic organs. (cdc.gov)
  • Hierarchical Screening of Pathways: Using Cell and Organ Cultures to Reduce use of Transgenic Mice. (nc3rs.org.uk)
  • To develop an in vitro organ culture of rat intervertebral disc and evaluate effects of TGF-beta3 on disc cell function. (nih.gov)
  • We describe a three-tissue organ-on-a-chip system, comprised of liver, heart, and lung, and highlight examples of inter-organ responses to drug administration. (nature.com)
  • A variety of adult organs (e.g. the liver) have been cultured using special media with special apparatus (Towell's II culture chamber). (wikipedia.org)
  • Here we use molecular techniques to generate transgenic cultures. (tu-dresden.de)
  • Serious attempts at the culture of whole tissues and isolated cells were first undertaken in the early 1900s as a technique for investigating the behavior of animal cells in an isolated and highly controlled environment. (fsu.edu)
  • The bactericidal activity of contaminated standard organ culture medium containing 100 IU/ml penicillin, 0.1 mg/ml streptomycin, and 0.25 μg/ml amphotericin B was evaluated after 48 hours of incubation at 31°C with five inocula of 14 bacteria. (bmj.com)
  • You are also expected to be a good teacher, as most people in the research team do not have a background in biology and so it is critical that the person filling this position will be able to support team members in several on-going research projects and collaborations on the topic of on-chip cell cultures. (uu.se)
  • To define the role of GLUT1 in vascular biology, we established cultured vascular smooth muscle cells (VSMCs) with constitutive upregulation of GLUT1, which led to a threefold increase in glucose uptake as well as significant increases in both nonoxidative and oxidative glucose metabolism as assessed by 13 C-nuclear magnetic resonance spectroscopy. (diabetesjournals.org)
  • For the last forty years, biologists have used traditional 2D cell culture to forge breakthroughs in cell biology. (labx.com)
  • Gene delivery by viral vectors in primary cultures of lacrimal gland tissue. (curehunter.com)
  • To test the feasibility of gene transfer into lacrimal gland tissue in primary culture, using different viral vectors. (curehunter.com)
  • Tissue was maintained under primary organ culture conditions using the "raft" technique. (curehunter.com)
  • Vaccinia and adenovirus are efficient vectors for gene transfer into lacrimal gland tissue in primary culture. (curehunter.com)
  • In addition, the culture was immunofluorescently labeled with Alexa Fluor 568 conjugated to goat secondary antibodies that target mouse anti-PDI (protein disulfide isomerase) primary antibodies (red fluorescence). (fsu.edu)
  • d Primary cell cultures. (nih.gov)
  • A bladder has been cultured by Anthony Atala of the Wake Forest Institute for Regenerative Medicine in Winston-Salem, North Carolina. (wikipedia.org)
  • This technique uses mucosae excised from sheep paranasal sinuses or pig bladder, which is cultured under controlled conditions. (spiedigitallibrary.org)
  • Another project deals with the analysis of cell and organ cultures with respect of their hormonal and more recently secondary metabolite status. (tu-dresden.de)
  • Moscona, A. & Moscona, H. The dissociation and aggregation of cells from organ rudiments of the early chick embryo. (nature.com)
  • Tissue morphology was studied using routine histologic, histochemical and immunohistochemical techniques. (nih.gov)
  • After 1 week in culture with TGF-beta3 and TGF-beta1, the gross morphology and tissue architecture of the disc were preserved. (nih.gov)
  • In vitro cultures of the cultivar 'Lakatan' were provided by the IPB in vitro germplasm collection for banana in 1995. (fao.org)
  • The global cell and tissue culture supplies market is driven by factors such as increasing investments on R&D for cell-based research and tissue engineering, growing demand for artificial organs, rising need for serums and vaccines, and among others. (medgadget.com)
  • An artificial kidney has been cultured by H. David Humes at the University of Michigan. (wikipedia.org)
  • Scientist makes materials used in tissue scaffolds to grow artificial human organs outside the body for implantation. (sciencephoto.com)
  • A jawbone has been cultured at Columbia University, a lung has been cultured at Yale. (wikipedia.org)
  • This report describes the two-dimensional culture and spheroid culture and the morphological comparison will be performed between two-dimensional culture and spheroid culture. (intechopen.com)
  • The aim of this review was to describe the two- and three-dimensional cultures, and the morphological comparison will be performed between two-dimensional culture and spheroid culture. (intechopen.com)
  • 6. A lettuce plant regenerated from the tissue culture of claim 3 , wherein the plant has all of the morphological and physiological characteristics of cultivar 40-0401159-B, wherein a representative sample of seed was deposited under ATCC Accession No. PTA-8604. (google.com)
  • Cronauer SS, Krikorian AD (1988d) Temporal, spatial and morphological aspects of multiplication in aseptically cultured Musa clones. (springer.com)
  • 6. A soybean plant regenerated from the tissue culture of claim 3, wherein the plant has all of the morphological and physiological characteristics of cultivar 1486018. (patentgenius.com)
  • Complexity of cell culture techniques and moral concern over mismanagement of animals may hamper the market growth. (medgadget.com)
  • The growth is attributed to rising need for automated cell culture process, increasing demand for drug discovery and increasing number of people suffer from chronic diseases. (medgadget.com)
  • The invention discloses a cell culture support which provides for the adhesion and culturing of one or more adhesive cells using a photoresist in which to provide a particular patterned design on a surface of the support. (google.com)
  • Further, the cell culture support is produced by pretreating the support surface with a reagent to provide hydrophobicity to the support surface. (google.com)
  • Also a reagent can be added to pretreat the support surface in order to facilitate adhesion at the photoresist prior to applying the photoresist into the cell culture support. (google.com)
  • In addition using a solution to promote film formation of a different film composition and thickness than the first is applied to produce the cell culture support. (google.com)
  • By varying the film composition a patterned design, such as checker board, is developed for purposes of providing a cell culture support for adherent cells. (google.com)
  • iv) dissolving the photoresist remaining on the support surface from (iii) to produce said cell culture support. (google.com)
  • 2. The cell culture support of claim 1 wherein the partial removal of the photoresist by photolithography is effected by treatment of the photoresist on the cell culture support surface by irradiation through a photomask and developing a irradiated photoresist under conditions which result in a predetermined patterning of the photoresist on the cell culture support surface. (google.com)
  • 3. The cell culture support of claim 1 wherein the cell culture support surface is pretreated with a reagent which facilitates adhesion at the photoresist prior to applying the photoresist into the cell culture support surface. (google.com)
  • 4. The cell culture support of claim 1 wherein the photoresist is removed by photolithography to provide for a striped, checkerboard or dotted pattern on the cell culture support surface. (google.com)
  • 5. The cell culture support of claim 1 wherein the photoresist layer is dissolved by treatment with an organic solvent and the cell culture support surface is then washed with water. (google.com)
  • 6. The cell culture support of claim 1 wherein the support contains cells adhered thereto. (google.com)
  • Cell Culture in an important tool for research and development within the life science industry. (smi-online.co.uk)
  • OThe global 3D Cell Culture Market market is valued at USD 1069.28 Million and is expected to grow with a CAGR of 25.31% over the forecast period. (smi-online.co.uk)
  • Researchers globally are realizing the potential of 3D cell culture for various applications, including models for personalized medicine, complex and multi-cell type models, translation and clinical and industrial applications. (smi-online.co.uk)
  • Over the past few years, 3D Cell Culture has gained momentum within the pharmaceutical industry due to the benefits that this model offers for in vitro applications patient-derived tissues, drug discovery, predictivity and validation, and safety and toxicity. (smi-online.co.uk)
  • SMi's 3D Cell Culture conference will explore advances of organ and lab-on-a-chip, microphysiological systems, applications of technology and case studies, imaging, high throughput screening and advances in 3D cell culture models which make up core components within the 3D Cell Culture field. (smi-online.co.uk)
  • The following article originally appeared on November 11, 2019 in The Cell Culture Dish here . (corning.com)
  • Our expertise in cell culture, 3-dimensional tissue constructs, and problem solving has fueled our group to offer the best options for individual testing needs. (iivs.org)
  • You will take a leading role in the research projects' cell culture activities, where you will be expected to run your own on-chip studies and closely communicate with our collaborators. (uu.se)
  • In recent years, huge steps have been made to standardize and manufacture equipment for 3D cell culture-making it cheaper, simpler and more reproducible. (labx.com)
  • This protocol assays cell migration between the developing gonad and kidney using tissue recombination, combined with an organ culture technique. (bio-medicine.org)
  • We will assess the fates available to each line in an organotypic context and will assess their ability to produce renal structures, up to a whole kidney, in novel tissue engineering culture systems based on our current state-of-the-art. (nc3rs.org.uk)
  • 2011). An improved kidney dissociation and reaggregation culture system results in nephrons arranged organotypically around a single duct system. (nc3rs.org.uk)
  • This technology generally relies on traditional three-dimensional culture techniques that exploit cell-autonomous self-organization responses of hPSCs with minimal control over the external inputs supplied to the system. (nature.com)
  • We have applied a recently reported technique of embryo explant culture to the analysis of neuronal development and migration in the zebrafish hindbrain. (zfin.org)
  • These results suggest that hindbrain explant culture can be employed effectively in zebrafish to analyze neuronal migration and other dynamic processes using pharmacological and imaging techniques. (zfin.org)
  • One reason for such poor translation from drug candidate to successful use is a lack of model systems that accurately recapitulate normal tissue function of human organs and their response to drug compounds. (nature.com)
  • Few engineered model systems, including the growing variety of organoid and organ-on-a-chip platforms, have so far reflected the interactive nature of the human body. (nature.com)
  • In the EMBLA research group, led by Dr. Maria Tenje, one of our research foci is development of microfluidic systems for organ-on-chip applications. (uu.se)
  • To strengthen the team, we are now looking to recruit a postdoc with a biological/medical background to focus on cell cultures in the different barrier-on-chip systems we develop. (uu.se)
  • Your main responsibilities will be to carry out and communicate top level scientific research in 1) on-chip cell cultures and 2) biological evaluation of barrier-on-chip microfluidic systems. (uu.se)
  • This month's issue of Cold Spring Harbor Protocols ( www.cshprotocols.org/TOCs/toc11_08.dtl ) features two articles with detailed instructions for setting up these experimental culture systems. (bio-medicine.org)
  • It was observed that overexpression of Id3 inhibited development of B cells in both culture systems. (bloodjournal.org)
  • In the attempt to avoid these disadvantages, transepithelial CXL (TE-CXL) technique was introduced, with a protocol based on a specially formulated riboflavin solution, Ricrolin TE (Sooft, Montegiorgio, FM, Italy), featuring two enhancers, trometamol and sodium EDTA, in order to improve stromal penetration. (hindawi.com)
  • The native stromal cells and extracellular matrix are typically removed, which enables isolated culture of the epithelial tissues. (nih.gov)
  • CD34 + CD38 − FL cells were transduced with Id3-IRES-GFP and cultured with the murine stromal cell line S17. (bloodjournal.org)
  • 3. To study controlling factors on extracellular matrix or stromal formation we have developed scaffold-free long-term cultures and these have recently enabled us to study the role of hypoxia, which occurs in a diurnal pattern in this tissue as one sleeps. (bu.edu)
  • However, the number of vegetative propagation cycles for the shoot-tip technique used may not be sufficient to eliminate chimeras completely in the mutated populations. (fao.org)
  • Cronauer SS, Krikorian AD (1985b) Reinitiation of vegetative growth from aseptically cultured terminal floral apex of banana. (springer.com)
  • Toward an optimum system for intervertebral disc organ culture: TGF-beta 3 enhances nucleus pulposus and anulus fibrosus survival and function thro. (nih.gov)
  • and (3) upregulation of gelatinases parallels vascular SMC migration and proliferation in rat and pig balloon-injured carotid arteries 22 24 25 and in organ cultures of human saphenous vein. (ahajournals.org)
  • In detail, spheroid culture produces an increased secretion of cytokines, such as vascular endothelial growth factor and granulocyte colony stimulating factor, when compared with that from two-dimensional cultures [ 6 ]. (intechopen.com)
  • Many tools and techniques have been developed for organoid culture, however depending on the cell type, research area and experimental goals, it can be difficult to identify the right method. (corning.com)
  • The focus of this session was to get an overview of the latest experimental contributions made to MicroBiology and Medical Research by Synchrotron based microanalysis and imaging techniques. (diamond.ac.uk)
  • A cousin of Rita's, Eugenia Sacerdote, also trained with Giuseppe Levi, and eventually introduced tissue culture techniques and the polio vaccine to Argentina. (asmblog.org)
  • Parts of an organ or a whole organ can be cultured in vitro. (wikipedia.org)
  • a Whole-organ and organ-slice cultures. (nih.gov)
  • Histologists study the organization of tissues at all levels, from the whole organ down to the molecular components of cells. (thefreedictionary.com)
  • The culture of adult organs or parts from adult animals is more difficult due to their greater requirement of oxygen. (wikipedia.org)
  • We argue that our novel brainstem culture model offers a powerful tool for the studies of axonal growth, neurogenesis, and regeneration in the adult zebrafish central nervous system. (zfin.org)
  • In banana, several DNA marker techniques have been used to investigate genetic relationships between Musa accessions, and to determine differences in somaclonal variants and radiation-induced mutants. (fao.org)
  • We describe here a model system that allows us to apply molecular genetic techniques to the analysis of human B-cell development. (bloodjournal.org)
  • Researchers at Carnegie Mellon University (CMU) and Nanyang Technological University (NTU) in Singapore have developed an organ-on-an-electronic-chip biosensor technology that can measure the electrophysiology of heart cell structures in three dimensions. (genengnews.com)
  • Ellman's Reagent or the GSH-400 assay were used to assay glutathione levels in lenses cultured for different times and under a variety of altered culture conditions. (elsevier.com)
  • The results obtained could provide a sound basis for the successful application of mutation and molecular marker techniques to improve bananas in the Philippines. (fao.org)
  • Induced mutation (either by chemicals or irradiation), coupled with in vitro propagation technique such as shoot-tip culture, has been established as a tool to generate variation in a number of vegetatively propagated crops [2-4]. (fao.org)
  • The project successfully adapted established protocols for mutation induction and subsequent propagation of in vitro cultured shoot tips [15] in two Philippine banana cultivars. (fao.org)
  • 7 Platelet-derived growth factor (PDGF) is 1 such factor, the secretion of which is increased in animal 8 and organ culture 9 models of neointima formation in veins. (ahajournals.org)
  • Fresh bovine respiratory tissues obtained from healthy cattle will be used to establish nasal and tracheal organ culture infection models. (nc3rs.org.uk)
  • Despite advances to date, organ-on-a-chip models still lack many of the elements of normal human tissue. (nature.com)
  • Scientists at the University of Cambridge have succeeded in growing miniature functional models of the lining of the womb (uterus) in culture. (eurekalert.org)
  • Replacing Animal Models: A Practical Guide to Creating and Using Culture-Based Biomimetic Alternatives 81-87. (nc3rs.org.uk)
  • Tissue and organ cultures are a promising approach in replacing cost intensive animal models in early stages of drug development. (spiedigitallibrary.org)
  • In parallel to this process, the bacteria are characterized by classic and/or molecular techniques. (wur.nl)
  • Molecular techniques are also increasingly being used for diagnostic purposes. (wur.nl)
  • 2) The regulation of organ and body size in insects. (duke.edu)
  • Tympanal organs occur in just about any part of the insect: the thorax, the base of the wing, the abdomen, the legs, etc., depending on the group of insects. (wikipedia.org)