A technique to generate restriction maps from single large DNA molecules by spreading the DNA onto a glass surface, digesting with DNA RESTRICTION ENZYMES, staining with FLUORESCENT DYES, and visualizing the DNA cleavage sites by FLUORESCENCE MICROSCOPY.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Any method used for determining the location of and relative distances between genes on a chromosome.
Techniques of nucleotide sequence analysis that increase the range, complexity, sensitivity, and accuracy of results by greatly increasing the scale of operations and thus the number of nucleotides, and the number of copies of each nucleotide sequenced. The sequencing may be done by analysis of the synthesis or ligation products, hybridization to preexisting sequences, etc.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
The systematic study of the complete DNA sequences (GENOME) of organisms.
Celestial bodies orbiting around the sun or other stars.
Genes whose entire sequences are contained within other genes.
The genetic complement of an organism, including all of its GENES, as represented in its DNA, or in some cases, its RNA.
The genetic complement of a BACTERIA as represented in its DNA.

High-resolution human genome structure by single-molecule analysis. (1/2)

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Clustering acinetobacter strains by optical mapping. (2/2)

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Optical restriction mapping is not a recognized medical term, but it is commonly used in the field of molecular biology and genetics. It refers to a technique used for mapping the location and arrangement of specific sequences or regions within a DNA molecule based on the pattern of restriction enzyme cleavage sites. The method uses fluorescent labeling and optical imaging techniques to visualize the resulting fragments, allowing researchers to determine the order and distance between different restriction sites.

In summary, optical restriction mapping is not a medical term, but rather a scientific technique used in molecular biology and genetics for mapping specific sequences or regions within a DNA molecule based on the pattern of restriction enzyme cleavage sites using fluorescent labeling and optical imaging techniques.

Restriction mapping is a technique used in molecular biology to identify the location and arrangement of specific restriction endonuclease recognition sites within a DNA molecule. Restriction endonucleases are enzymes that cut double-stranded DNA at specific sequences, producing fragments of various lengths. By digesting the DNA with different combinations of these enzymes and analyzing the resulting fragment sizes through techniques such as agarose gel electrophoresis, researchers can generate a restriction map - a visual representation of the locations and distances between recognition sites on the DNA molecule. This information is crucial for various applications, including cloning, genome analysis, and genetic engineering.

DNA restriction enzymes, also known as restriction endonucleases, are a type of enzyme that cut double-stranded DNA at specific recognition sites. These enzymes are produced by bacteria and archaea as a defense mechanism against foreign DNA, such as that found in bacteriophages (viruses that infect bacteria).

Restriction enzymes recognize specific sequences of nucleotides (the building blocks of DNA) and cleave the phosphodiester bonds between them. The recognition sites for these enzymes are usually palindromic, meaning that the sequence reads the same in both directions when facing the opposite strands of DNA.

Restriction enzymes are widely used in molecular biology research for various applications such as genetic engineering, genome mapping, and DNA fingerprinting. They allow scientists to cut DNA at specific sites, creating precise fragments that can be manipulated and analyzed. The use of restriction enzymes has been instrumental in the development of recombinant DNA technology and the Human Genome Project.

Chromosome mapping, also known as physical mapping, is the process of determining the location and order of specific genes or genetic markers on a chromosome. This is typically done by using various laboratory techniques to identify landmarks along the chromosome, such as restriction enzyme cutting sites or patterns of DNA sequence repeats. The resulting map provides important information about the organization and structure of the genome, and can be used for a variety of purposes, including identifying the location of genes associated with genetic diseases, studying evolutionary relationships between organisms, and developing genetic markers for use in breeding or forensic applications.

High-throughput nucleotide sequencing, also known as next-generation sequencing (NGS), refers to a group of technologies that allow for the rapid and parallel determination of nucleotide sequences of DNA or RNA molecules. These techniques enable the sequencing of large numbers of DNA or RNA fragments simultaneously, resulting in the generation of vast amounts of sequence data in a single run.

High-throughput sequencing has revolutionized genomics research by allowing for the rapid and cost-effective sequencing of entire genomes, transcriptomes, and epigenomes. It has numerous applications in basic research, including genome assembly, gene expression analysis, variant detection, and methylation profiling, as well as in clinical settings, such as diagnosis of genetic diseases, identification of pathogens, and monitoring of cancer progression and treatment response.

Some common high-throughput sequencing platforms include Illumina (sequencing by synthesis), Ion Torrent (semiconductor sequencing), Pacific Biosciences (single molecule real-time sequencing), and Oxford Nanopore Technologies (nanopore sequencing). Each platform has its strengths and limitations, and the choice of technology depends on the specific research question and experimental design.

DNA Sequence Analysis is the systematic determination of the order of nucleotides in a DNA molecule. It is a critical component of modern molecular biology, genetics, and genetic engineering. The process involves determining the exact order of the four nucleotide bases - adenine (A), guanine (G), cytosine (C), and thymine (T) - in a DNA molecule or fragment. This information is used in various applications such as identifying gene mutations, studying evolutionary relationships, developing molecular markers for breeding, and diagnosing genetic diseases.

The process of DNA Sequence Analysis typically involves several steps, including DNA extraction, PCR amplification (if necessary), purification, sequencing reaction, and electrophoresis. The resulting data is then analyzed using specialized software to determine the exact sequence of nucleotides.

In recent years, high-throughput DNA sequencing technologies have revolutionized the field of genomics, enabling the rapid and cost-effective sequencing of entire genomes. This has led to an explosion of genomic data and new insights into the genetic basis of many diseases and traits.

Genomics is the scientific study of genes and their functions. It involves the sequencing and analysis of an organism's genome, which is its complete set of DNA, including all of its genes. Genomics also includes the study of how genes interact with each other and with the environment. This field of study can provide important insights into the genetic basis of diseases and can lead to the development of new diagnostic tools and treatments.

I'm sorry for any confusion, but "planets" is not a medical term. It is a term used in astronomy to refer to celestial bodies that orbit the sun and meet certain criteria, such as having cleared their orbit of other debris. If you have any questions about medical terminology or concepts, I'd be happy to try to help!

"Nested genes" is not a widely recognized or formally defined term in genetics or molecular biology. It may refer to a situation where one gene is completely contained within the intron (non-coding region) of another gene. This phenomenon is also known as "gene-within-a-gene" or "overlapping genes."

In some cases, nested genes can be transcribed together as a single transcript, which then undergoes alternative splicing to produce different mature mRNAs (messenger RNA) for each gene. This allows for the efficient use of genetic material and increases the genetic complexity of an organism without requiring additional DNA sequences.

However, it is important to note that the study of nested genes is still a developing area of research, and the mechanisms and functional significance of this phenomenon are not yet fully understood.

A genome is the complete set of genetic material (DNA, or in some viruses, RNA) present in a single cell of an organism. It includes all of the genes, both coding and noncoding, as well as other regulatory elements that together determine the unique characteristics of that organism. The human genome, for example, contains approximately 3 billion base pairs and about 20,000-25,000 protein-coding genes.

The term "genome" was first coined by Hans Winkler in 1920, derived from the word "gene" and the suffix "-ome," which refers to a complete set of something. The study of genomes is known as genomics.

Understanding the genome can provide valuable insights into the genetic basis of diseases, evolution, and other biological processes. With advancements in sequencing technologies, it has become possible to determine the entire genomic sequence of many organisms, including humans, and use this information for various applications such as personalized medicine, gene therapy, and biotechnology.

A bacterial genome is the complete set of genetic material, including both DNA and RNA, found within a single bacterium. It contains all the hereditary information necessary for the bacterium to grow, reproduce, and survive in its environment. The bacterial genome typically includes circular chromosomes, as well as plasmids, which are smaller, circular DNA molecules that can carry additional genes. These genes encode various functional elements such as enzymes, structural proteins, and regulatory sequences that determine the bacterium's characteristics and behavior.

Bacterial genomes vary widely in size, ranging from around 130 kilobases (kb) in Mycoplasma genitalium to over 14 megabases (Mb) in Sorangium cellulosum. The complete sequencing and analysis of bacterial genomes have provided valuable insights into the biology, evolution, and pathogenicity of bacteria, enabling researchers to better understand their roles in various diseases and potential applications in biotechnology.

Ordered restriction maps of Saccharomyces cerevisiae chromosomes constructed by optical mapping. Science 262, 110-114 (1993). ... Nature 475, 348-352 (2011). This paper describes the first non-optical sequencing technology using a massively parallel semi- ... Rapid genome mapping in nanochannel arrays for highly complete and accurate de novo sequence assembly of the complex Aegilops ... Genome mapping on nanochannel arrays for structural variation analysis and sequence assembly. Nat. Biotechnol. 30, 771-776 ( ...
... high-resolution restriction maps from single, stained molecules of DNA, called "optical maps". By mapping the location of ... This produces an optical map of single molecules. Individual optical maps are combined to produce a consensus, genomic optical ... Initially, the optical mapping system has been used to construct whole-genome restriction maps of bacteria, parasites, and ... "Ordered Restriction Maps of Saccharomyces Cerevisiae Chromosomes Constructed by Optical Mapping." Science 262.5130 (1993): 110- ...
High-resolution restriction maps of bacterial artificial chromosomes constructed by optical mapping. W Cai, J Jing, B Irvin, L ... A shotgun optical map of the entire Plasmodium falciparum genome. Z Lai, J Jing, C Aston, V Clarke, J Apodaca, ET Dimalanta, DJ ... Automated high resolution optical mapping using arrayed, fluid-fixed DNA molecules. J Jing, J Reed, J Huang, X Hu, V Clarke, J ... Whole-genome shotgun optical mapping of Deinococcus radiodurans. J Lin, R Qi, C Aston, J Jing, TS Anantharaman, B Mishra, O ...
Genome-wide optical maps are ordered high-resolution restriction maps that give the position of occurrence of restriction cut ... Kermit: linkage map guided long read assembly With long reads getting even longer and cheaper, large scale sequencing projects ... sites corresponding to one or more restriction enzymes. These genome-wide optical m... ...
Here, we review several algorithms and methods for building consensus optical maps and aligning restriction patterns to a ... Optical mapping and newer genome mapping technologies based on nicking enzymes provide low resolution but long-range genomic ... The optical mapping technique has been successfully used for assessing the quality of genome assemblies and for detecting large ... reference map, as well as methods for using optical maps with sequence assemblies.. ...
Optical mapping. Optical maps [38] for each genome using the restriction enzymes AflII and NheI (Y. aldovae and Y. kristensenii ... Alignment of contigs to the optical maps [39] suggested that the optical maps consistently overestimated sizes (2 to 10% on ... Nagarajan N, Read TD, Pop M: Scaffolding and validation of bacterial genome assemblies using optical restriction maps. ... We also derived an independent estimate for the genome size from the whole-genome optical restriction mapping of the species [ ...
... a whole-genome restriction map, White said. "We make a fingerprint of the DNA, of the whole genome, that we can then put into ... "so that the contigs can be mapped back to the complete ordered optical map scaffold of DNA, giving researchers the ability to ... The Optical Mapping Technology was licensed from New York University and the University of Wisconsin, and OpGen has been ... For strain typing, an optical map of the isolate of interest can be generated in about 24 hours, compared to the days or weeks ...
S. Saha and S. Rajasekaran, Efficient and scalable scaffolding using optical restriction maps, BMC Genomics 2014, 15(Suppl 5): ... H. ElGindy and S. Rajasekaran, Sorting and Selection Algorithms on a Linear Array with Optical Bus System, Parallel Processing ... M. Song and S. Rajasekaran, A Transaction Mapping Algorithm for Frequent Itemsets Mining, IEEE Transactions on Knowledge and ... S. Rajasekaran and S. Sahni, Sorting, Selection, and Routing on the Array with Reconfigurable Optical Buses, IEEE Transactions ...
We suggest that the areal border imposes a strong restriction on the pattern formation of orientation preference maps in the ... In this study we examined the arrangement of orientation pinwheel centers in the cat visual cortex by optical imaging of ... We suggest that the areal border imposes a strong restriction on the pattern formation of orientation preference maps in the ... We suggest that the areal border imposes a strong restriction on the pattern formation of orientation preference maps in the ...
has restriction * bronze Date in CU Experts * February 25, 2021 5:10 AM ... Space Telescope and Optical Reverberation Mapping Project. IX. Velocity-Delay Maps for Broad Emission Lines in NGC 5548 Journal ...
Optical genome mapping (OGM), which allows analysis of ultra-high molecular weight (UHMW) DNA molecules, represents a response ... to the restriction created by short-read next-generation-sequencing, even in cases where the causative variant is a neutral ... Case Report: Decrypting an interchromosomal insertion associated with Marfans syndrome: how optical genome mapping emphasizes ...
Ordered restriction endonuclease maps of yeast artificial chromosomes created by optical mapping on surfaces. Proc Natl Acad ... High-resolution physical mapping by combined Alu-hybridization/PCR screening: construction of a yeast artificial chromosome map ... 1993) Isolation and FISH mapping of 80 cosmid clones on the short arm of human chromosome 3. Genomics 16: 90-6 ...
In one case, water restrictions were lifted from a group of users based on Landsat data used with the states METRIC model (a ... The Nations largest wholesaler of water, the U.S. Bureau of Reclamation, uses Landsat data for mapping and monitoring on the ... At the state level, Landsats unique combination of thermal and optical data makes for an efficient tool for fair enforcement ... In Australia, Landsat imagery, in conjunction with images from NASAs MODIS and commercial satellites, has been used to map ...
Automated Optical Tweezers Manipulation to Transfer Mitochondria from Fetal to Adult MSCs to Improve Antiaging Gene Expressions ... Neural Degeneration in Normal-Aging Human Cochleas: Machine-Learning Counts and 3D Mapping in Archival Sections November 14, ... Calorie restriction reduces biomarkers of cellular senescence in humans November 14, 2023 ...
Inventory of potentially misassembled sequences identified by the optical map data. (XLSX) ... Map of the tropical Asia Pacific region showing the sampling locations for Asian seabass across its native range. India-Western ... Evaluation of the Asian seabass scaffolded genome assembly (v2) by mapping Illumina PE Genome reads to assembly for linear ... Condition-Specific Mapping of Operons (COSMO) using dynamic and static genome data ...
Optical Restriction Mapping. Mapeo de Restricción Optica. Matrizes de Pontuação de Posição Específica. Position-Specific ...
Optical Restriction Mapping. Mapeamento por Restrição Óptica. Mapeo de Restricción Optica. Photoelectron Spectroscopy. ... Epicardial Mapping. Mapeamento Epicárdico. Mapeo Epicárdico. Four-Dimensional Computed Tomography. Tomografia Computadorizada ...
Optical Restriction Mapping. Mapeamento por Restrição Óptica. Mapeo de Restricción Optica. Photoelectron Spectroscopy. ... Epicardial Mapping. Mapeamento Epicárdico. Mapeo Epicárdico. Four-Dimensional Computed Tomography. Tomografia Computadorizada ...
Optical Restriction Mapping. Mapeo de Restricción Optica. Matrizes de Pontuação de Posição Específica. Position-Specific ...
Optical Restriction Mapping. Mapeamento por Restrição Óptica. Mapeo de Restricción Optica. Photoelectron Spectroscopy. ... Epicardial Mapping. Mapeamento Epicárdico. Mapeo Epicárdico. Four-Dimensional Computed Tomography. Tomografia Computadorizada ...
Optical Restriction Mapping. Mapeamento por Restrição Óptica. Mapeo de Restricción Optica. Photoelectron Spectroscopy. ... Epicardial Mapping. Mapeamento Epicárdico. Mapeo Epicárdico. Four-Dimensional Computed Tomography. Tomografia Computadorizada ...
Optical Restriction Mapping. Mapeo de Restricción Optica. Matrizes de Pontuação de Posição Específica. Position-Specific ...
Optical Restriction Mapping. Mapeamento por Restrição Óptica. Mapeo de Restricción Optica. Photoelectron Spectroscopy. ... Epicardial Mapping. Mapeamento Epicárdico. Mapeo Epicárdico. Four-Dimensional Computed Tomography. Tomografia Computadorizada ...
Optical Restriction Mapping. Mapeamento por Restrição Óptica. Mapeo de Restricción Optica. Photoelectron Spectroscopy. ... Epicardial Mapping. Mapeamento Epicárdico. Mapeo Epicárdico. Four-Dimensional Computed Tomography. Tomografia Computadorizada ...
Optical Restriction Mapping. Mapeamento por Restrição Óptica. Mapeo de Restricción Optica. Photoelectron Spectroscopy. ... Epicardial Mapping. Mapeamento Epicárdico. Mapeo Epicárdico. Four-Dimensional Computed Tomography. Tomografia Computadorizada ...
Optical Restriction Mapping. Mapeamento por Restrição Óptica. Mapeo de Restricción Optica. Photoelectron Spectroscopy. ... Epicardial Mapping. Mapeamento Epicárdico. Mapeo Epicárdico. Four-Dimensional Computed Tomography. Tomografia Computadorizada ...
Optical Restriction Mapping. Mapeo de Restricción Optica. Matrizes de Pontuação de Posição Específica. Position-Specific ...
Multi-dimensional pooling of 295,680 BAC clones and sequencing the ends of restriction fragments of pooled DNA generated 1,814 ... The physical map provides a framework for assembly of genome sequence and information for localization of genes that are ... The physical map provides a framework for assembly of genome sequence and information for localization of genes that are ... WGPTM technology has proved to provide a robust physical map of the pea genome, which would have been difficult to assemble ...
They will be moderately priced and will be able to produce a good-quality heat map. Without financial restrictions, you can ... Most experts note the optimal refresh rate of 50 Hz, the presence of optical and digital zoom, the high resolution of the OLED ... It has a 50Hz refresh rate, 1300 meters detection range, 1.5x optical zoom, and up to 4x digital zoom. Complements all this ... However, in most cases, 4x-5x optical and 6x-8x digital zoom are considered ideal. There are TS and twice the performance. ...

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