Ophiopogon
Analysis of homoisoflavonoids in Ophiopogon japonicus by HPLC-DAD-ESI-MSn. (1/9)
The homoisoflavonoids in Ophiopogon japonicus (Thunb.) Ker-Gawler were analyzed by high-performance liquid chromatography-diode array detection-electrospray ion trap tandem mass spectrometry (HPLC-DAD-ESI-MSn). Homoisoflavonoids gave prominent [M - H]- ions by electrospray ionization monitored in the negative ion mode. They could be classified into two types depending on the fragmentation behavior of their [M - H]- ions in the ion trap mass analyzer. The [M - H]- ions of homoisoflavonoids with a saturated C2-3 bond underwent C3-9 bond cleavage to lose the B-ring, which was followed by the loss of a molecule of CO. The [M - H]- ions of homoisoflavonoids with a C2-3 double bond usually eliminated a CO molecule first, and then underwent the cleavage of C3-9 or C9-1' bonds. For homoisoflavonoids with a C-6 formyl group, however, the neutral loss of CO was the first fragmentation step; the presence of a methoxyl group at C-8 could lead to the cleavage of C-ring. No retro Diels-Alder (RDA) fragmentation characteristic for normal flavonoids was observed. The above fragmentation rules were reported for the first time, and were implemented for the analysis of homoisoflavonoids in O. japonicus. The CHCl3-MeOH extract was separated on a Zorbax Extend-C18 column, eluting with a acetonitrile-0.3% acetic acid gradient. A total of 18 homoisoflavonoids, including seven new minor constituents, were identified or tentatively characterized based on the UV spectra and tandem mass spectra of the HPLC peaks. (+info)Antithrombotic activities of aqueous extract from Radix Ophiopogon japonicus and its two constituents. (2/9)
To provide further pharmacological evidence for its clinical use in thrombotic diseases, the antithrombotic activities of the aqueous extract of Radix Ophiopogon japonicus (ROJ-ext) were studied in mouse and rat models. The results showed that ROJ-ext remarkably decreased length of tail thrombus in mice at 48 h and 72 h after carrageenan injection at doses of 12.5 and 25.0 mg/kg. Meanwhile, ROJ-ext markedly inhibited thrombosis induced by arterial-venous (AV) shunt (silk thread) in rats at doses of 6.25 and 12.5 mg/kg. Furthermore, ROJ-ext and one of its components, ruscogenin, significantly inhibited platelet aggregation induced by adenosine diphosphate (ADP) in rats by oral administration of 12.5 mg/kg or 0.7 mg/kg for three times, however, ophiopogonin D 1.4 mg/kg only showed slight inhibition. On the other hand, ophiopogonin D (0.5-2.0 mg/kg, p.o.) and ruscogenin (0.25-1.00 mg/kg, p.o.) produced dose-related inhibition of venous thrombosis induced by tight ligation of the inferior vena cava for 6 h in mice by once oral administration. The findings of this study indicate that an aqueous extract of Radix Ophiopogon japonicus (ROJ-ext) exerted significant antithrombotic activity and ruscogenin and ophiopogonin D are two of its active components, which supported its therapeutic use for thrombotic diseases. (+info)Possible mechanism of the anti-inflammatory activity of ruscogenin: role of intercellular adhesion molecule-1 and nuclear factor-kappaB. (3/9)
Ruscogenin (RUS), first isolated from Ruscus aculeatus, also a major steroidal sapogenin of traditional Chinese herb Radix Ophiopogon japonicus, has been found to exert significant anti-inflammatory and anti-thrombotic activities. Our previous studies suggested that ruscogenin remarkably inhibited adhesion of leukocytes to a human umbilical vein endothelial cell line (ECV304) injured by tumor necrosis factor-alpha (TNF-alpha) in a concentration-dependent manner. Yet the underlying mechanisms remain unclear. In this study, the in vivo effects of ruscogenin on leukocyte migration and celiac prostaglandin E(2) (PGE(2)) level induced by zymosan A were studied in mice. Furthermore, the effects of ruscogenin on TNF-alpha-induced intercellular adhesion molecule-1 (ICAM-1) expression and nuclear factor-kappaB (NF-kappaB) activation were also investigated under consideration of their key roles in leukocyte recruitment. The results showed that ruscogenin significantly suppressed zymosan A-evoked peritoneal total leukocyte migration in mice in a dose-dependent manner, while it had no obvious effect on PGE(2) content in peritoneal exudant. Ruscogenin also inhibited TNF-alpha-induced over expression of ICAM-1 both at the mRNA and protein levels and suppressed NF-kappaB activation considerably by decreasing NF-kappaB p65 translocation and DNA binding activity. These findings provide some new insights that may explain the possible molecular mechanism of ruscogenin and Radix Ophiopogon japonicus for the inhibition of endothelial responses to cytokines during inflammatory and vascular disorders. (+info)New neuritogenic steroidal saponin from Ophiopogon japonicus (Thunb.) Ker-Gawl. (4/9)
A new steroidal saponin was isolated from Ophiopogon japonicus. This saponin possesses a modification by 2-hydroxy-3-methylvalerylation of the hydroxyl group at C-4' of the sugar, linked to C-1 of the aglycone. It exhibited significant neuritogenic activity for PC12 cells. The structure-activity relationship revealed the aglycone, rather than the sugar moieties and acylation, to be important for the neuritogenic activity. (+info)Long-circulating delivery of bioactive polysaccharide from radix ophiopogonis by PEGylation. (5/9)
(+info)SCAR markers for discriminating species of two genera of medicinal plants, Liriope and Ophiopogon. (6/9)
The development of DNA markers that can closely discriminate between Liriope and Ophiopogon species is vital for efficient and accurate identification of these species, and to ensure the quality, safety, and efficacy of medicines made from these plants. We developed species-specific molecular markers for these two genera. Forty RAPD primers were tested to detect polymorphism; species-specific RAPD bands were gel-purified, cloned, and sequenced. Primers for sequence-characterized amplified regions (SCARs) were then designed, based on nucleotide sequences of specific RAPD primers. SCAR markers SA06 and SB05, specific to Ophiopogon japonicus, amplified 460- and 553-bp DNA fragments, respectively. The marker SA12 amplified a 485-bp fragment specific to Liriope platyphylla. This is the first report of a species-specific SCAR marker for this group. These markers will be useful for rapid identification of closely related Liriope and Ophiopogon species. (+info)Antimicrobial activities of endophytic fungi isolated from Ophiopogon japonicus (Liliaceae). (7/9)
(+info)The functional significance of black-pigmented leaves: photosynthesis, photoprotection and productivity in Ophiopogon planiscapus 'Nigrescens'. (8/9)
(+info)Ophiopogon is a genus of small, evergreen perennial plants native to Asia, often used in traditional medicine, whose species are characterized by their narrow, grass-like leaves and tuberous roots, some of which contain medicinal sap used to treat various conditions such as mouth sores, heart ailments, and diabetes, although scientific evidence supporting these uses is generally limited.
"Ophiopogon" is a botanical term that refers to a genus of plants commonly known as "mondo grass" or "snake's beard." While it is not a medical term, some species of Ophiopogon have been used in traditional medicine in certain cultures. For example, Ophiopogon japonicus (also known as "dwarf lilyturf") has been used in Traditional Chinese Medicine for its supposed anti-inflammatory and antioxidant properties. However, it's important to note that the use of Ophiopogon species in modern medicine is not well-researched or widely accepted, and more scientific evidence is needed to support their medicinal benefits.
I'm sorry for any confusion, but Liriope plant is not a medical term and does not have a medical definition; it is a type of ornamental plant also known as lilyturf, often used in landscaping, with several species including Liriope muscari and Liriope spicata.
I'm sorry for any confusion, but "Liriope" is actually not a medical term, but a botanical name. Liriope is a genus of perennial plants that are native to warm temperate and subtropical regions of eastern Asia. Common names for Liriope include lilyturf or monkey grass. These plants are often used in gardening and landscaping due to their attractive, grass-like foliage and their ability to form dense, spreading mats.
If you have any questions about a medical condition or treatment, I would be happy to try to help!