A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.
Short fragments of DNA that are used to alter the function of target RNAs or DNAs to which they hybridize.
Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible.
Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)
Flammable, amorphous, vegetable products of secretion or disintegration, usually formed in special cavities of plants. They are generally insoluble in water and soluble in alcohol, carbon tetrachloride, ether, or volatile oils. They are fusible and have a conchoidal fracture. They are the oxidation or polymerization products of the terpenes, and are mixtures of aromatic acids and esters. Most are soft and sticky, but harden after exposure to cold. (From Grant & Hackh's Chemical Dictionary, 5th ed & Dorland, 28th ed)
5-Bromo-2,4(1H,3H)-pyrimidinedione. Brominated derivative of uracil that acts as an antimetabolite, substituting for thymine in DNA. It is used mainly as an experimental mutagen, but its deoxyriboside (BROMODEOXYURIDINE) is used to treat neoplasms.
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
Short fragments of DNA or RNA that are used to alter the function of target RNAs or DNAs to which they hybridize.
An enzyme that catalyzes the conversion of linear RNA to a circular form by the transfer of the 5'-phosphate to the 3'-hydroxyl terminus. It also catalyzes the covalent joining of two polyribonucleotides in phosphodiester linkage. EC 6.5.1.3.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
A series of steps taken in order to conduct research.
A purine or pyrimidine base bonded to DEOXYRIBOSE.
Organic compounds that contain phosphorus as an integral part of the molecule. Included under this heading is broad array of synthetic compounds that are used as PESTICIDES and DRUGS.
Carbon-containing phosphoric acid derivatives. Included under this heading are compounds that have CARBON atoms bound to one or more OXYGEN atoms of the P(=O)(O)3 structure. Note that several specific classes of endogenous phosphorus-containing compounds such as NUCLEOTIDES; PHOSPHOLIPIDS; and PHOSPHOPROTEINS are listed elsewhere.
A form of SILICON DIOXIDE composed of skeletons of prehistoric aquatic plants which is used for its ABSORPTION quality, taking up 1.5-4 times its weight in water. The microscopic sharp edges are useful for insect control but can also be an inhalation hazard. It has been used in baked goods and animal feed. Kieselguhr is German for flint + earthy sediment.
Nucleotides in which the base moiety is substituted with one or more sulfur atoms.
Catalyze the joining of preformed ribonucleotides or deoxyribonucleotides in phosphodiester linkage during genetic processes. EC 6.5.1.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
A ribonuclease that specifically cleaves the RNA moiety of RNA:DNA hybrids. It has been isolated from a wide variety of prokaryotic and eukaryotic organisms as well as RETROVIRUSES.
Californium. A man-made radioactive actinide with atomic symbol Cf, atomic number 98, and atomic weight 251. Its valence can be +2 or +3. Californium has medical use as a radiation source for radiotherapy.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A purine or pyrimidine base bonded to a DEOXYRIBOSE containing a bond to a phosphate group.
2'-Deoxyuridine. An antimetabolite that is converted to deoxyuridine triphosphate during DNA synthesis. Laboratory suppression of deoxyuridine is used to diagnose megaloblastic anemias due to vitamin B12 and folate deficiencies.
Short fragments of RNA that are used to alter the function of target RNAs or DNAs to which they hybridize.
Inorganic derivatives of phosphoric acid (H3PO4). Note that organic derivatives of phosphoric acids are listed under ORGANOPHOSPHATES.
A purine base and a fundamental unit of ADENINE NUCLEOTIDES.
Compounds containing carbon-phosphorus bonds in which the phosphorus component is also bonded to one or more sulfur atoms. Many of these compounds function as CHOLINERGIC AGENTS and as INSECTICIDES.
Hard, amorphous, brittle, inorganic, usually transparent, polymerous silicate of basic oxides, usually potassium or sodium. It is used in the form of hard sheets, vessels, tubing, fibers, ceramics, beads, etc.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
Biologically functional sequences of DNA chemically synthesized in vitro.
Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.
Pairing of purine and pyrimidine bases by HYDROGEN BONDING in double-stranded DNA or RNA.
A DNA-dependent DNA polymerase characterized in prokaryotes and may be present in higher organisms. It has both 3'-5' and 5'-3' exonuclease activity, but cannot use native double-stranded DNA as template-primer. It is not inhibited by sulfhydryl reagents and is active in both DNA synthesis and repair. EC 2.7.7.7.
DNA that is complementary to the sense strand. (The sense strand has the same sequence as the mRNA transcript. The antisense strand is the template for mRNA synthesis.) Synthetic antisense DNAs are used to hybridize to complementary sequences in target RNAs or DNAs to effect the functioning of specific genes for investigative or therapeutic purposes.
A series of 7 virulent phages which infect E. coli. The T-even phages T2, T4; (BACTERIOPHAGE T4), and T6, and the phage T5 are called "autonomously virulent" because they cause cessation of all bacterial metabolism on infection. Phages T1, T3; (BACTERIOPHAGE T3), and T7; (BACTERIOPHAGE T7) are called "dependent virulent" because they depend on continued bacterial metabolism during the lytic cycle. The T-even phages contain 5-hydroxymethylcytosine in place of ordinary cytosine in their DNA.
A nucleoside consisting of the base guanine and the sugar deoxyribose.
A single chain of deoxyribonucleotides that occurs in some bacteria and viruses. It usually exists as a covalently closed circle.
Macromolecular molds for the synthesis of complementary macromolecules, as in DNA REPLICATION; GENETIC TRANSCRIPTION of DNA to RNA, and GENETIC TRANSLATION of RNA into POLYPEPTIDES.
A group of ribonucleotides (up to 12) in which the phosphate residues of each ribonucleotide act as bridges in forming diester linkages between the ribose moieties.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
Purine or pyrimidine bases attached to a ribose or deoxyribose. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.
The rate dynamics in chemical or physical systems.
Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
The covalent bonding of an alkyl group to an organic compound. It can occur by a simple addition reaction or by substitution of another functional group.
A rigorously mathematical analysis of energy relationships (heat, work, temperature, and equilibrium). It describes systems whose states are determined by thermal parameters, such as temperature, in addition to mechanical and electromagnetic parameters. (From Hawley's Condensed Chemical Dictionary, 12th ed)
The relative amounts of the PURINES and PYRIMIDINES in a nucleic acid.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
Reagents with two reactive groups, usually at opposite ends of the molecule, that are capable of reacting with and thereby forming bridges between side chains of amino acids in proteins; the locations of naturally reactive areas within proteins can thereby be identified; may also be used for other macromolecules, like glycoproteins, nucleic acids, or other.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
Organic compounds containing the -CO-NH2 radical. Amides are derived from acids by replacement of -OH by -NH2 or from ammonia by the replacement of H by an acyl group. (From Grant & Hackh's Chemical Dictionary, 5th ed)
That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.
Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
A family of 6-membered heterocyclic compounds occurring in nature in a wide variety of forms. They include several nucleic acid constituents (CYTOSINE; THYMINE; and URACIL) and form the basic structure of the barbiturates.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.

Id helix-loop-helix proteins inhibit nucleoprotein complex formation by the TCF ETS-domain transcription factors. (1/10948)

The Id subfamily of helix-loop-helix (HLH) proteins plays a fundamental role in the regulation of cellular proliferation and differentiation. Id proteins are thought to inhibit differentiation mainly through interaction with other HLH proteins and by blocking their DNA-binding activity. Members of the ternary complex factor (TCF) subfamily of ETS-domain proteins have key functions in regulating immediate-early gene expression in response to mitogenic stimulation. TCFs form DNA-bound complexes with the serum response factor (SRF) and are direct targets of MAP kinase (MAPK) signal transduction cascades. In this study we demonstrate functional interactions between Id proteins and TCFs. Ids bind to the ETS DNA-binding domain and disrupt the formation of DNA-bound complexes between TCFs and SRF on the c-fos serum response element (SRE). Inhibition occurs by disrupting protein-DNA interactions with the TCF component of this complex. In vivo, the Id proteins cause down-regulation of the transcriptional activity mediated by the TCFs and thereby block MAPK signalling to SREs. Therefore, our results demonstrate a novel facet of Id function in the coordination of mitogenic signalling and cell cycle entry.  (+info)

Oligomerization and scaffolding functions of the erythropoietin receptor cytoplasmic tail. (2/10948)

Signal transduction by the erythropoietin receptor (EPOR) is activated by ligand-mediated receptor homodimerization. However, the relationship between extracellular and intracellular domain oligomerization remains poorly understood. To assess the requirements for dimerization of receptor cytoplasmic sequences for signaling, we overexpressed mutant EPORs in combination with wild-type (WT) EPOR to drive formation of heterodimeric (i.e. WT-mutant) receptor complexes. Dimerization of the membrane-proximal portion of the EPOR cytoplasmic region was found to be critical for the initiation of mitogenic signaling. However, dimerization of the entire EPOR cytoplasmic region was not required. To examine this process more closely, we generated chimeras between the intracellular and transmembrane portions of the EPOR and the extracellular domains of the interleukin-2 receptor beta and gammac chains. These chimeras allowed us to assess more precisely the signaling role of each receptor chain because only heterodimers of WT and mutant receptor chimeras form in the presence of interleukin-2. Coexpression studies demonstrated that a functional receptor complex requires the membrane-proximal region of each receptor subunit in the oligomer to permit activation of JAK2 but only one membrane-distal tail to activate STAT5 and to support cell proliferation. Thus, this study defines key relationships involved in the assembly and activation of the EPOR signal transduction complex which may be applicable to other homodimeric cytokine receptors.  (+info)

Localization of curved DNA and its association with nucleosome phasing in the promoter region of the human estrogen receptor alpha gene. (3/10948)

We determined DNA bend sites in the promoter region of the human estrogen receptor (ER) gene by the circular permutation assay. A total of five sites (ERB-4 to -1, and ERB+1) mapped in the 3 kb region showed an average distance of 688 bp. Most of the sites were accompanied by short poly(dA) x poly(dT) tracts including the potential bend core sequence A2N8A2N8A2 (A/A/A). Fine mapping of the ERB-2 site indicated that this A/A/A and the 20 bp immediate flanking sequence containing one half of the estrogen response element were the sites of DNA curvature. All of the experimentally mapped bend sites corresponded to the positions of DNA curvature as well as to nucleosomes predicted by computer analysis. In vitro nucleosome mapping at ERB-2 revealed that the bend center was located 10-30 bp from the experimental and predicted nucleosome dyad axes.  (+info)

Chlamydia infections and heart disease linked through antigenic mimicry. (4/10948)

Chlamydia infections are epidemiologically linked to human heart disease. A peptide from the murine heart muscle-specific alpha myosin heavy chain that has sequence homology to the 60-kilodalton cysteine-rich outer membrane proteins of Chlamydia pneumoniae, C. psittaci, and C. trachomatis was shown to induce autoimmune inflammatory heart disease in mice. Injection of the homologous Chlamydia peptides into mice also induced perivascular inflammation, fibrotic changes, and blood vessel occlusion in the heart, as well as triggering T and B cell reactivity to the homologous endogenous heart muscle-specific peptide. Chlamydia DNA functioned as an adjuvant in the triggering of peptide-induced inflammatory heart disease. Infection with C. trachomatis led to the production of autoantibodies to heart muscle-specific epitopes. Thus, Chlamydia-mediated heart disease is induced by antigenic mimicry of a heart muscle-specific protein.  (+info)

The RNA-editing enzyme ADAR1 is localized to the nascent ribonucleoprotein matrix on Xenopus lampbrush chromosomes but specifically associates with an atypical loop. (5/10948)

Double-stranded RNA adenosine deaminase (ADAR1, dsRAD, DRADA) converts adenosines to inosines in double-stranded RNAs. Few candidate substrates for ADAR1 editing are known at this point and it is not known how substrate recognition is achieved. In some cases editing sites are defined by basepaired regions formed between intronic and exonic sequences, suggesting that the enzyme might function cotranscriptionally. We have isolated two variants of Xenopus laevis ADAR1 for which no editing substrates are currently known. We demonstrate that both variants of the enzyme are associated with transcriptionally active chromosome loops suggesting that the enzyme acts cotranscriptionally. The widespread distribution of the protein along the entire chromosome indicates that ADAR1 associates with the RNP matrix in a substrate-independent manner. Inhibition of splicing, another cotranscriptional process, does not affect the chromosomal localization of ADAR1. Furthermore, we can show that the enzyme is dramatically enriched on a special RNA-containing loop that seems transcriptionally silent. Detailed analysis of this loop suggests that it might represent a site of ADAR1 storage or a site where active RNA editing is taking place. Finally, mutational analysis of ADAR1 demonstrates that a putative Z-DNA binding domain present in ADAR1 is not required for chromosomal targeting of the protein.  (+info)

Multiple oligodeoxyribonucleotide syntheseson a reusable solid-phase CPG support via the hydroquinone-O,O'-diacetic acid (Q-Linker) linker arm. (6/10948)

A strategy for oligodeoxyribonucleotide synthesis on a reusable CPG solid-phase support, derivatized with hydroxyl groups instead of amino groups, has been developed. Ester linkages, through a base labile hydroquinone- O, O '-diacetic acid ( Q-Linker ) linker arm, were used to couple the first nucleoside to the hydroxyl groups on the support. This coupling was rapidly accomplished (10 min) using O -benzotriazol-1-yl- N, N, N ', N '-tetramethyluronium hexafluorophosphate (HBTU) and 1-hydroxybenzotriazole as the activating reagents. Oligodeoxyribonucleotide synthesis was performed using existing procedures and reagents, except a more labile capping reagent, such as chloro-acetic anhydride, methoxyacetic anhydride or t-butylphenoxyacetic anhydride, was used instead of acetic anhydride. After each oligodeoxyribonucleotide synthesis, the product was cleaved from the support with ammonium hydroxide (3 min) and deprotected as usual. Residual linker arms or capping groups were removed by treatment with ammonium hydroxide/methylamine reagent and the regenerated support was capable of reuse. Up to six different oligodeoxyribonucleotide syntheses or up to 25 cycles of nucleoside derivatization and cleavage were consecutively performed on the reusable support. This method may provide a significant cost advantage over conventional single-use solid supports currently used for the manufacture of antisense oligodeoxyribonucleotides.  (+info)

Mutational analysis of Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase propeptide processing. (7/10948)

Glutamine phosphoribosylpyrophosphate amidotransferase from Bacillus subtilis is a member of an N-terminal nucleophile hydrolase enzyme superfamily, several of which undergo autocatalytic propeptide processing to generate the mature active enzyme. A series of mutations was analyzed to determine whether amino acid residues required for catalysis are also used for propeptide processing. Propeptide cleavage was strongly inhibited by replacement of the cysteine nucleophile and two residues of an oxyanion hole that are required for glutaminase function. However, significant propeptide processing was retained in a deletion mutant with multiple defects in catalysis that was devoid of enzyme activity. Intermolecular processing of noncleaved mutant enzyme subunits by active wild-type enzyme subunits was not detected in hetero-oligomers obtained from a coexpression experiment. While direct in vitro evidence for autocatalytic propeptide cleavage was not obtained, the results indicate that some but not all of the amino acid residues that have a role in catalysis are also needed for propeptide processing.  (+info)

Hybridization of antisense oligonucleotides with the 3'part of tRNA(Phe). (8/10948)

The interaction of antisense oligodeoxyribonucleotides with yeast tRNA(Phe) was investigated. 14-15-mers complementary to the 3'-terminal sequence including the ACCA end bind to the tRNA under physiological conditions. At low oligonucleotide concentrations the binding occurs at the unique complementary site. At higher oligonucleotide concentrations, the second oligonucleotide molecule binds to the complex due to non-perfect duplex formation in the T-loop stabilized by stacking between the two bound oligonucleotides. In these complexes the acceptor stem is open and the 5'-terminal sequence of the tRNA is accessible for binding of a complementary oligonucleotide. The results prove that the efficient binding of oligonucleotides to the 3'-terminal sequence of the tRNA occurs through initial binding to the single-stranded sequence ACCA followed by invasion in the acceptor stem and strand displacement.  (+info)

The invention consists of oligonucleotides which inhibit the immunostimulatory activity of ISS-ODN (immunostimulatory sequence oligodeoxynucleotides) as well as methods for their identification and use. The oligonucleotides of the invention are useful in controlling therapeutically intended ISS-ODN adjuvant activity as well as undesired ISS-ODN activity exerted by recombinant expression vectors, such as those used for gene therapy and gene immunization. The oligonucleotides of the invention also have anti-inflammatory activity useful in reducing inflammation in response to infection of a host with ISS-ODN containing microbes, in controlling autoimmune disease and in boosting host Th2 type immune responses to an antigen. The invention also encompasses pharmaecutically useful conjugates of the oligonucleotides of the invention (including conjugate partners such as antigens and antibodies).
Page contains details about 3-tocopherol-modified 1826 CpG oligonucleotide micelle-SIINFEKL conjugates . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Synthetic oligodeoxynucleotides (ODN) containing unmethylated CpG motifs act as immune adjuvants, accelerating and boosting antigen-specific immune responses. CpG motifs promote the induction of Th1 and pro-inflammatory cytokines and support the maturation/activation of professional antigen presenti …
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Oligodeoxyribonucleotides containing CpG motifs (CpG ODNs) are widely used for activation of immune cells, such as human PBMCs, murine splenocytes or isolated immune cells, e.g., B cells and pDCs. They also activate signalling in TLR9-expressing recombinant cell lines. ODN 1826 strongly activates murine TLR9. It is a B-class ODN for activation of B cells, induction of Il-6 secretion, and activation of NF-kB-signalling pathways. - USA
Oligodeoxyribonucleotides containing CpG motifs (CpG ODNs) are widely used for activation of immune cells, such as human PBMCs, murine splenocytes or isolated immune cells, e.g., B cells and pDCs. They also activate signalling in TLR9-expressing recombinant cell lines. ODN 1826 strongly activates murine TLR9. It is a B-class ODN for activation of B cells, induction of Il-6 secretion, and activation of NF-kB-signalling pathways. - Belgique
CpG oligodeoxynucleotide treatment enhances innate resistance and acquired immunity to African trypanosomes.by Harris TH, Mansfield JM, Paulnock DM. MiniManuscript.
Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5-phosphate and 3-OH groups.. The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ ions.. ...
Immunostimulatory oligonucleotide (ISS-ODN) used as adjuvants are commonly modified with phosphorothioate (PS). the transient effects from a PO TLR-9 agonist may be beneficial for protection in a bacterial bioterrorism attack by delaying the onset of systemic contamination without the induction of a cytokine CCT239065 syndrome. activity due to a short half-life [11 12 Their shorter half-life may limit their potency but also their potential toxicity. However several studies have shown that specific modifications of PO ISS-ODN improve their efficacy as adjuvants. Examples of bioeffective alterations include conjugation of the PO ISS-ODN to a hexameric deoxyriboguanosine (3′dG6) at the 3′-end [13] and chemically linking two PO ISS-ODN via their 3′ ends. We recently reported the structural requirements for PO ISS-ODN to penetrate cells and to elicit a functional TLR-9 response [14 15 Serial selection from a random library and optimal structural modifications resulted in the generation of a PO ...
The invention relates to novel modified oligonucleotides, the construction thereof, and their use in oligonucleotide-based therapies. More specifically, the invention is to novel oligonucleotides having modified internucleoside linkages which are resistant to nucleases, having enhanced ability to penetrate cells, and which are capable of binding target oligonucleotide sequences in vitro and in vivo. The modified oligonucleotides of the invention are particularly useful in oligonucleotide-based therapies utilizing the modified oligonucleotides to interrupt protein synthesis or transcription or to otherwise inactivate messenger RNA or double stranded DNA.
Despite considerable therapeutic potential of stimulatory ODNs, their application in the therapy and identification of several types of ODNs, the rational design of TLR9 agonists has been limited by a lack of clear information on the structural parameters that define the minimal ligand features required for the TLR9 activation. This changed recently by the determination of the crystal structures of horse and mouse TLR9 with bound B class short ODNs (7). This structure pointed to the recognition of a single CpG by the receptor, although our previous study identified a clear requirement for a pair of CpG required for the activation of hTLR9. Although the CpG motif represents the consensus recognition motif of all TLR9, its sequence selectivity is species-specific (1, 8-12). In this paper, we identified the minimal sequence motifs of ODNs for the effective and specific activation of mouse receptor. In summary, the minM may be defined by the sequence pattern [T]4-6CG[T]12-21. However, the activation ...
Abstract. CpG ODN are being actively investigated as cancer vaccine adjuvants because they mature plasmacytoid dendritic cells (pDC) into potent antigen-presen
DNA synthesis: Oligodeoxynucleotides were synthesized on a Cyclone Plus DNA Synthesizer (Millipore, Marlborough, MA) using standard phosphoramidite chemistry. Precursor phosphoramidites were purchased from PerSeptive Biosystems (Farmington, MA) or from Glenn Research (Sterling, VA). They were then purified using Oligo-Pure cartridges (Hamilton, Reno, NV) according to the manufacturers protocol. This was followed by 32P end-labelling, as previously described (Smith, et al. 1991). Mobility Shift Assays: Duplexes were formed by combining equimolar (20 µM) amounts of complementary strands in annealing buffer (10 mM Tris-HCl pH 7.4, 1 mM EDTA, and 100 mM NaCl) then treating with 95°C for 5 minutes and 50°C for 60 minutes. The samples were allowed to cool to room temperature for 10 min. and were then stored on ice until needed. Methyltransferase Purification: M·HhaI was obtained via purification from E. coli RR1 containing the pSP72 plasmid (Promega, Madison, WI) carrying the entire HhaI ...
InvivoGen offers a wide range of high-quality TLR9 ligands in three classes: CpG ODN, inhibitory ODNs and immunostimulatory bacterial DNA. All of these ligands that mimic unmethylated CpG are functionally validated in hTLR9 cells. Some CpG ODNs are available with sterility and endotoxin certificatio
117D: Crystal and molecular structure of the alternating dodecamer d(GCGTACGTACGC) in the A-DNA form: comparison with the isomorphous non-alternating dodecamer d(CCGTACGTACGG).
After testing the ODNs, an effective ODN or ODNs are chosen; a rough guideline for an effective ODN is one which depletes the target mRNA to less than 20% of the level of the uninjected at a 10 ng dose, as seen by northern analysis. The ODNs so selected are generally resynthesized in a modified form. The modification suggested by experiments of Baker, et al. (1990) and Dagle, et al. (1990) is used, where 3-4 of the 5-most and 3-most phosphodiester bonds are replaced by phosphorothioate bonds, leaving at least 8 unmodified bonds in the center of the ODN (e.g. Kofron et al., 1997). Modified oligodeoxynucleotides provide two major advantages over unmodified ODNs. The modified ODNs can deplete the message more effectively than the unmodified ODNs at a much lower concentration (e.g. compare the 2 ng depletion by modified ODN #7 in fig. 4b to the 10 ng unmodified ODN #7 depletion in fig. 4a). Second, the modified ODNs provide a more reliable and reproducible depletion. However, it has been our ...
The Centers for Disease http://53alpha.com/generic-renova-online/ Control and Prevention (CDC) health scientist Vikram Krishnasamy, M. D, renova spa cancun prices MPH, has been registered by the Coronavirus Response and Relief Supplemental Appropriations Act. Despite more than 1,000 campers and staff from nearly every state and 7 countries, only three people tested positive for COVID-19 symptoms. We are renova spa cancun prices deeply saddened by the Centers for Medicare and Medicaid Services (CMS) and the latest science may convince even more to do so.. The Centers for Disease Control and Prevention (CDC) issued a Framework for Conditional Sailing Order that introduces a phased approach for the CDC estimate that on any given day in 2018, 1 in 5 people in the year after pregnancy. The Centers for Disease Control and Prevention (CDC) show that adult obesity prevalence is increasing and racial and ethnic disparities persist. Transcript for the safe and responsible resumption of passenger renova ...
TY - JOUR. T1 - CpG oligonucleotides enhance the tumor antigen-specific immune response of a granulocyte macrophage colony-stimulating factor-based vaccine strategy in neuroblastoma. AU - Sandler, Anthony D.. AU - Chihara, Hiroshi. AU - Kobayashi, Gen. AU - Zhu, Xiaoyan. AU - Miller, Michal A.. AU - Scott, David. AU - Krieg, Arthur M.. PY - 2003/1/15. Y1 - 2003/1/15. N2 - Granulocyte macrophage colony-stimulating factor (GM-CSF)-transduced autologous tumor cells form the basis of many immunotherapeutic strategies. We tested whether combining this approach with T-helper 1 (Th-1)-like immunostimulatory CpG oligodeoxynucleotides (CpG ODNs) would improve therapeutic efficacy in an established model of murine neuroblastoma. The weakly immunogenic Neuro-2a cell line was used in syngeneic A/J mice. CpG 1826 was tested for its antitumor effect alone and as an adjuvant to Neuro-2a cells retrovirally transduced to express murine GM-CSF (GM/Neuro-2a). Three days after wild-type (WT) tumor cell inoculation, ...
92118DNAArtificial SequenceA synthetic DNA fragment 1aaggagcgat cgccatgn 18210DNAArtificial SequenceA synthetic DNA fragment, wherein nnn is the first codon which is 3 to the start codon followed by the remainder of an open reading frame 2cgccatgnnn 10312DNAArtificial SequenceA synthetic DNA fragment 3nnnnnngtct tc 12410DNAArtificial SequenceA synthetic DNA fragment 4nnnngaagag 10513DNAArtificial SequenceA synthetic DNA fragment 5gcagcnnnnn nnn 13611DNAArtificial SequenceA synthetic DNA fragment 6nnnnngagac g 11711DNAArtificial SequenceA synthetic DNA fragment 7gccnnnnngg c 11814DNAArtificial SequenceA synthetic DNA fragment 8ggatgnnnnn nnnn 14911DNAArtificial SequenceA synthetic DNA fragment 9nnnnngagac c 111010DNAArtificial SequenceA synthetic DNA fragment 10gacgcnnnnn 101111DNAArtificial SequenceA synthetic DNA fragment 11ccnnnnnnng g 111211DNAArtificial SequenceA synthetic DNA fragment 12gcnnnnnnng c 111310DNAArtificial SequenceA synthetic DNA fragment 13nnnnngagac 101411DNAArtificial ...
Oligonucleotides are short nucleic acid polymers used in research, genetic testing and forensics. Oligonucleotides are usually made up of 13 to 25 nucleotides and are designed to hybridize specifically to DNA or RNA sequences. Solid-phase clinical synthesis is used to manufacture these small bits of nucleic acid for use in polymerase chain reaction (PCR), DNA sequencing, library construction and artificial gene synthesis.. Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure/sequence.In the enzymatic process of DNA synthesis, deoxy-ribonucleotide-5-tri-phosphates are used as substrates during polymerization. In addition, this process requires a primer and a template.. Full Research Report On Global Oligonucleotide Synthesis Market Analysis available @ https://www.millioninsights.com/industry-reports/oligonucleotide-synthesis-market. The most frequently used oligonucleotide synthesis strategy is based upon ...
Oligonucleotides are short nucleic acid polymers used in research, genetic testing and forensics. Oligonucleotides are usually made up of 13 to 25 nucleotides and are designed to hybridize specifically to DNA or RNA sequences. Solid-phase clinical synthesis is used to manufacture these small bits of nucleic acid for use in polymerase chain reaction (PCR), DNA sequencing, library construction and artificial gene synthesis.. Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure/sequence.In the enzymatic process of DNA synthesis, deoxy-ribonucleotide-5-tri-phosphates are used as substrates during polymerization. In addition, this process requires a primer and a template.. Full Research Report On Global Oligonucleotide Synthesis Market Analysis available @ https://www.millioninsights.com/industry-reports/oligonucleotide-synthesis-market. The most frequently used oligonucleotide synthesis strategy is based upon ...
In this study, we report the development of a novel, rationally designed immunostimulatory adjuvant based on chemical conjugation of CpG oligodeoxynucleotide (ODN) to the nontoxic B subunit of cholera toxin (CTB). We demonstrate that the immunostimulatory effects of CpG can be dramatically enhanced by conjugation to CTB. Thus, CpG ODN linked to CTB (CTB-CpG) was shown to be a more potent stimulator of proinflammatory cytokine and chemokine responses in murine splenocytes and human PBMCs than those of CpG ODN alone in vitro. The presence of CpG motif, but not modified phosphorothioate ODN backbone, was found to be critical for the enhanced immunostimulatory effects of CTB-CpG. Our mode-of-action studies, including studies on cells from specifically gene knockout mice suggest that similar to CpG, CTB-CpG exerts its immunostimulatory effects through a TLR9/MyD88- and NF-kappaB-dependent pathway. Surprisingly, and as opposed to CpG ODN, CTB-CpG-induced immunity was shown to be independent of ...
Disclosed is a method for detecting a nucleic acid target sequence by formation of triple helix nucleic acid structures. The method may, but need not, involve amplifying the nucleic acid in vitro using cycles of denaturation and amplification to yield product duplexes, and detecting the product duplexes by hybridizing a third strand of nucleic acid to the product duplexes without denaturation. The triple helix-forming duplex sequences may be endogenous to the target sequence being detected, or they may be introduced in the probes used during amplification.
TY - GEN. T1 - Therapeutic applications and mechanisms underlying the activity of immunosuppressive oligonucleotides. AU - Klinman, Dennis M.. AU - Tross, Debbie. AU - Klaschik, Sven. AU - Shirota, Hidekazu. AU - Sato, Takeshi. N1 - Copyright: Copyright 2018 Elsevier B.V., All rights reserved.. PY - 2009/9/1. Y1 - 2009/9/1. N2 - Synthetic oligodeoxynucleotides (ODN) capable of neutralizing or inhibiting immune responses have been described. This review will focus on the properties of phosphorothioate ODN that mimic the immunosuppressive activity of the repetitive TTAGGG motifs present in mammalian telomeres. These TTAGGG multimers block the production of pro-inflammatory and T helper type 1 cytokines elicited when immune cells are activated by a wide variety of Toll-like receptor ligands, polyclonal activators, and antigens. Several mechanisms contribute to the suppressive activity of such ODN. Ongoing microarray studies indicate that suppressive ODN interfere with the phosphorylation of ...
Microbial DNA sequences containing unmethylated CpG dinucleotides activate Toll-like receptor 9 (TLR9). We have found that TLR9 is localized to the endoplasmic reticulum (ER) of dendritic cells (DCs) and macrophages. Because there is no precedent for immune receptor signaling in the ER, we investigated how TLR9 is activated. We show that CpG DNA binds directly to TLR9 in ligand-binding studies. CpG DNA moves into early endosomes and is subsequently transported to a tubular lysosomal compartment. Concurrent with the movement of CpG DNA in cells, TLR9 redistributes from the ER to CpG DNA-containing structures, which also accumulate MyD88. Our data indicate a previously unknown mechanism of cellular activation involving the recruitment of TLR9 from the ER to sites of CpG DNA uptake, where signal transduction is initiated.
CpG 2006 Biotin ODN (also known as CpG ODN 7909 Biotin) is a labeled human TLR9 ligand. InvivoGen CpG oligonucleotides are functionally tested and guaranteed endotoxin-free to avoid non-specific immune stimulation. The sequence of CpG-B (type K) ODN 2006 (also known as PF-3512676) is tcgtcgttttgtcgt
Animals, Antibodies/pharmacology, Arthritis/genetics/*immunology, CpG Islands/*immunology, Freunds Adjuvant, Oligodeoxyribonucleotides/immunology/*pharmacology, Rats, Rats; Inbred Lew, Receptors; Antigen; T-Cell; alpha-beta/immunology, T-Lymphocytes/*immunology ...
Oligonucleotides are single-stranded and short RNA or DNA molecule that have a wide range of applications in research forensics and genetic testing. Oligon
Degenerate DNA Oligonucleotides. Our team offers the synthesis of DNA oligonucleotides with equimolar mixtures of two or more different bases at a given position within the sequence. Synthesis scales of 50 nmole and 200 nmole are available. No extra charges apply. -------------------------------------------------------------------------------------------------------------------------------------------. Contact us! Please let us know what your individual research needs are. We can accommodate special requests that might not be explicitly listed here. We also offer free technical assistance to help you make the best choice for your specific application.. For a brief introduction of our DNA Oligonucleotide Synthesis unit and an overview of our products and services, please see here.. ...
Biocompatible materials with nano-scale structure hold great promise for controlled and targeted delivery and half-life extension of both small-molecule drugs and various classes of biologics, such as peptides, proteins, plasmid DNA and synthetic oligodeoxynucleotides. Promising delivery systems include microcapsules, liposomes, macromolecular conjugates, nanoparticles, dendrimers, and biological stuctures such polypeptides, benign viruses and bacteriophages. This symposium will focus on commercially promising new materials and approaches. ...
Terminal lipophilization of a unique DNA dodecamer by various nucleolipid headgroups: Their incorporation into artificial lipid bilayers and hydrodynamic properties
291995831 - EP 1212085 B1 2007-10-31 - COMPOSITIONS FOR STIMULATING CYTOKINE SECRETION AND INDUCING AN IMMUNE RESPONSE - [origin: WO0115726A2] Lipid-nucleic acid particles can provide therapeutic benefits, even when the nucleic acid is not complementary to coding sequences in target cells. It has been found that lipid-nucleic acid particles, including those containing non-sequence specific oligodeoxynucleotides, can be used to stimulate cytokine secretion, thus enhancing the overall immune response of a treated mammal. Further, immune response to specific target antigens can be induced by administration of an antigenic molecule in association with lipid particles containing non-sequence specific oligodeoxynucleotides. The nucleic acid which is included in the lipid-nucleic acid particle can be a phosphodiester (i.e., an oligodeoxynucleotide consisting of nucleotide residues joined by phosphodiester linkages) or a modified nucleic acid which includes phosphorothioate or other modified linkages, and may
Fast and reliable custom oligonucleotide synthesis service at competitive price. Bioneer is one of the worlds largest suppliers of synthetic oligonucleotides. Every Bioneer oligo is purified free-of-charge utilizing our unique Bio-RP cartridge purification technology and quality checked by MALDI-TOF.
5DNB: Structure of the B-DNA decamer C-C-A-A-C-G-T-T-G-G and comparison with isomorphous decamers C-C-A-A-G-A-T-T-G-G and C-C-A-G-G-C-C-T-G-G.
TY - JOUR. T1 - Phosphorothioate-modified oligodeoxynucleotides inhibit human cytomegalovirus replication by blocking virus entry. AU - Luganini, Anna. AU - Caposio, Patrizia. AU - Landolfo, Santo. AU - Gribaudo, Giorgio. PY - 2008/3. Y1 - 2008/3. N2 - Studies in animal models have provided evidence that Toll-like receptor 9 (TLR9) agonists, such as synthetic oligodeoxynucleotides (ODNs) that contain immunostimulatory deoxycytidyl-deoxyguanosine (CpG) motifs (CpG ODNs), protect against a wide range of viral pathogens. This antiviral activity has been suggested to be indirect and secondary to CpG-induced cytokines and inflammatory responses triggered through TLR9 activation. However, few studies have addressed the potential of CpG ODNs as direct antiviral agents. Here, we report on the ability of some CpG ODNs to directly suppress, almost completely, human cytomegalovirus (HCMV) replication in both primary fibroblasts and endothelial cells. Murine CMV replication was inhibited as well, whereas no ...
Novel compounds that mimic and/or modulate the activity of wild-type nucleic acids. In general, the compounds are phosphorothioate oligonucleotides wherein the 5′-terminal internucleoside linkage or the 5′- and 3′-terminal linkages are modified.
BOC RNA has been a leading manufacturer of phosphoramidites and oligonucleotides for a long time and has a wealth of expertise and experience in these fields. It can provide a variety of types of high-quality phosphoramidites for the production of oligonucleotides for clinical applications. The range of products and services offered by this company is very suitable for customers who have strict requirements or need to control the manufacturing process.. The automated chemical synthesis of oligonucleotides is essential for the production of primers for polymerase chain reaction (PCR), oligonucleotide-based drugs, and many other medical and biotechnological applications. The highly optimized automated chemical oligonucleotide synthesis relies on phosphoramidite as the phosphate precursor.. The nucleoside phosphoramidite was first described in 1981. Phosphoramidites are modified nucleosides and are standard chemicals used in modern times. These molecules allow for the addition of new base sequences ...
93119DNAArtificial sequenceSingle strand DNA oligonucleotide 1ttctcccttc tccctctgc 19218DNAArtificial sequenceSingle strand DNA oligonucleotide 2gtggtcccaa gacaatgc 18320DNAArtificial sequenceSingle strand DNA oligonucleotide 3cctgcctctg ttgagactcc 20427DNAArtificial sequenceSingle strand DNA oligonucleotide 4ttctgctaac agtattcttt aatgtga 27521DNAArtificial sequenceSingle strand DNA oligonucleotide 5tgaagagttt gattccgaac g 21618DNAArtificial sequenceSingle strand DNA oligonucleotide 6agccagggta tggctgct 18720DNAArtificial sequenceSingle strand DNA oligonucleotide 7cagcctgact ggacacagaa 20820DNAArtificial sequenceSingle strand DNA oligonucleotide 8atctactgtg cccagcgact 20924DNAArtificial sequenceSingle strand DNA oligonucleotide 9gaaaggatca tctctacttt ctgg 241019DNAArtificial sequenceSingle strand DNA oligonucleotide 10gtggcgagat gctagacag 191124DNAArtificial sequenceSingle strand DNA oligonucleotide 11attttgaact tgagcaggta gttg 241220DNAArtificial sequenceSingle strand DNA oligonucleotide ...
TY - JOUR. T1 - The use of degenerate, sensor gene-specific, oligodeoxyribonucleotide primers to amplify DNA fragments from Staphylococcus aureus. AU - Bayles, Kenneth W.. PY - 1993/1/15. Y1 - 1993/1/15. N2 - The sensor proteins of bacterial two-component regulatory systems comprise a large family of proteins that are involved in environmental sensing and signal transduction. To study these proteins in the Gram+ pathogen, Staphylococcus aureus, two pairs of degenerate oligodeoxyribonucleotides (oligos) that corresponded to conserved sequences contained within sensor protein-encoding genes were synthesized. Using these oligo primers, DNA fragments from S. aureus were amplified by polymerase chain reaction (PCR), cloned in Escherichia coli, and sequenced. Comparison of the deduced amino acid sequences from these cloned fragments to the sequences contained in the GenBank database suggest that some of the PCR products were derived from sensor protein-encoding genes. However, several other fragments ...
TY - JOUR. T1 - The use of degenerate, sensor gene-specific, oligodeoxyribonucleotide primers to amplify DNA fragments from Staphylococcus aureus. AU - Bayles, Kenneth W.. PY - 1993/1/15. Y1 - 1993/1/15. N2 - The sensor proteins of bacterial two-component regulatory systems comprise a large family of proteins that are involved in environmental sensing and signal transduction. To study these proteins in the Gram+ pathogen, Staphylococcus aureus, two pairs of degenerate oligodeoxyribonucleotides (oligos) that corresponded to conserved sequences contained within sensor protein-encoding genes were synthesized. Using these oligo primers, DNA fragments from S. aureus were amplified by polymerase chain reaction (PCR), cloned in Escherichia coli, and sequenced. Comparison of the deduced amino acid sequences from these cloned fragments to the sequences contained in the GenBank database suggest that some of the PCR products were derived from sensor protein-encoding genes. However, several other fragments ...
This unit provides a modified phosphoramidite method to synthesize oligodeoxyribonucleotides onto a universal and reusable hydroxyl solid support thanks to the use of deoxyribonucleoside tert‐butyl and cyanoethyl phosphoramidites
AIM: To determine the optimum form of labelling and the most efficient reporter molecule for non-radioisotopic in situ hybridisation (ISH). METHODS: Nine deoxyoligonucleotides complementary to histone mRNA were synthesised and labelled either enzymatically or during solid-phase synthesis with the reporter molecules digoxigenin, 2,4-dinitrophenyl (DNP), or alkaline phosphatase. Pooled deoxyoligonucleotide cocktails were then used in non-radioisotopic ISH detection of histone mRNA in human tonsil. Hybrid detection was by nitroblue tetrazoleum/5-bromo-4-chloro-3-indolyl phosphate colorimetric development. RESULTS: The use of a spacer in 3 enzymatic labelling and when labelling with alkaline phosphatase significantly increased ISH signal. The 3 and 5 labelling of oligonucleotides with triple DNP groups during solid-phase synthesis produced the strongest signal as determined by the highest cell signal intensity and shortest development time. CONCLUSIONS: 3 and 5 solid-phase labelling with triple ...
Oligonucleotide synthesis market segmented global market by product(Synthesized Oligonucleotides, Custom Oligonucleotides), application(Research, Therapeutics, Diagnostics), end users & region.
Descriptive info: Homepage.. Welcome to BioSpring.. BioSpring , The Oligo Company.. Expertise in oligonucleotide synthesis.. Flexibility by open source oligonucleotide production system.. Synthesis of oligonucleotides.. Standard analyses of oligonucleotides.. Additional analyses of oligonucleotides.. Superstructures: Biological significance and structure.. siRNA, RNAi.. Interfering RNA.. Hybridisation of siRNA molecules.. Quality and delivery time.. Phosphonoacetates.. Antisense molecules.. RNase H activity.. Transport into the cells.. Advantages of phosphonoacetates.. Scales and Modifications.. Unmodified oligonucleotides.. Phosphorothioates.. RNA.. Modified RNA.. Methylphosphonates.. Phosphonoacetate oligonucleotides (PACE).. RNAi, siRNA miRNA.. 5 modifications.. 3 modifications.. Internal modifications.. Dual labeled probes.. Additional services.. Delivery.. Qualification and validation.. BioSpring GMP Manufacturing movie.. Oligonucleotides for diagnostic applications.. Certificate ISO ...
There is evidence from mouse models that CpG DNA acts as a potent vaccine adjuvant for promoting Th1-like immune responses (2-7, 10, 29-32). In contrast, very little published data about CpG DNA effects on human cells are available (10, 28, 33), and no reports whether CpG DNA may activate human DCs, which is currently an area of great therapeutic interest, and where GMCSF has shown much promise. In the present report we demonstrate that CpG DNA is a more potent stimulus than GMCSF for inducing primary blood DC survival, differentiation, activation, maturation, and the functional ability to promote a Th1-like T cell response.. CpG DNA was superior to GMCSF in preserving in vitro survival of primary blood DCs and inducing differentiation, which was reflected by an increase in cell size, granularity, and MHC II expression. CpG treatment led to activation of DCs as represented by up-regulation of the costimulatory molecules ICAM-1 (CD54), B7-2 (CD86), and CD40 and to maturation indicated by ...
TY - JOUR. T1 - Chemical and structural characterization of the interaction of bleomycin A2 with d(CGCGAATTCGCG)2. Efficient, double-strand DNA cleavage accessible without structural reorganization. AU - Keck, M. V.. AU - Manderville, R. A.. AU - Hecht, S. M.. N1 - Copyright: Copyright 2011 Elsevier B.V., All rights reserved.. PY - 2001/9/12. Y1 - 2001/9/12. N2 - A detailed description of the interaction between Fe(II)·bleomycin A2 and the Dickerson-Drew dodecamer d(CGCGAATTCGCG)2 is presented. The reaction between bleomycin and this substrate leads to DNA cleavage at two major sites, adenosines and cytidine11, and two minor sites, cytidine3 and thymidines8. The pattern and relative intensities of cleavage at these sites was not entirely consistent with what would be predicted based on the preference of the drug for cleavage at the pyrimidines of 5′-GC-3′ and 5′-GT-3′ sites. Insight into the origins of the apparent alteration of selectivity was provided by examination of the structure ...
The present invention relates to synergistic combinations of immunostimulatory CpG oligonucleotides and immunopotentiating cytokines. In particular, the invention relates to methods of stimulating an immune response using the synergistic combination of compounds and products related thereto.
The applications of oligonucleotides are vast, even beyond the life science / pharma sector; however, owing to complexities associated with the synthesis and
We have proposed previously that the RIα regulatory subunit of PKA-I is an ontogenic growth-inducing protein and that its constitutive expression disrupts normal ontogenic processes, resulting in a pathogenic outgrowth such as cancer (2) . The results presented here confirm this view and suggest that the RIα antisense, which works through the Watson-Crick base pairing mechanism of action, can serve as a single gene-based therapeutic agent for cancer. The sequence-specific mechanism of action of RIα antisense is strongly supported by the experimental data that MBO antisense, having an increased hybridizing capacity and nuclease resistance, increased the antisense effect of growth inhibition, whereas the mismatched MBO control oligos could not mimic the antisense effects.. In this study, minimization of the polyanionic nature of the PS-oligo and modifications of the immunostimulatory CpG motif were two important goals for us to demonstrate the sequence-specific antisense effects in the absence ...
Coley Pharmaceutical, in collaboration with sanofi-aventis, is developing novel, immunostimulatory CpG toll-like receptor 9 (TLR9) agonists for the treatment of
Ziel dieses Arbeitsgebiets ist die präklinische Validierung von Decoy- Oligodesoxynukleotiden (ODN) als neue Wirkstoffklasse zur Prävention bzw. Therapie der Herzinsuffizienz. Decoy ODNs (decoy = Köder, Lockvogel) sind doppelsträngige, meist 15 bis 20 Basenpaare kurze synthestische DNA-Moleküle, die spezifisch die DNA-Bindungsstelle bestimmter Regulatorproteine (Transkriptionsfaktoren) im Genom nachahmen. Sie interferieren mit der zumeist aberranten Expression krankheitsrelevanter Gene, indem sie gezielt an den für die Expression dieser Gene verantwortlichen Transkriptionsfaktor binden und ihn damit hemmen. Drei verschiedene Transkriptionsfaktoren werden als potentielle therapeutische Zielmoleküle untersucht. Wichtigstes Kriterium für ihre Auswahl ist ihre nachgewiesene Beteiligung an der Expression von Genen, die für die Ausprägung der verschiedenen Varianten der terminalen Herzinsuffizienz primär verantwortlich zu sein scheinen ...
All current genetic testing methods require synthetic oligonucleotide primers and probes. At ATDBio we make high quality custom qPCR and LAMP primers and probes, working with our customers to help them develop simpler, faster and more accurate diagnostic methods. Were using our specialist nucleic acid expertise to accelerate the global response to the pandemic, helping to get the world back up and running quickly and safely. Weve already made oligos for millions of tests, and were scaling up even further. ...
Mouse TLR9-agonist kit; contains 100 ug of ODN1585-1; ODN1826-1, ODN2395-1, and negative controls ODN1585-1NC; ODN1826-1-1NC, ODN2395-1NC datasheet and description hight quality product and Backed by our Guarantee
Original Factory Issued Service Repair Manual for the Sansui Model TU-X701 DIGITAL SYNTHESIZER TUNER. Used, but in Very good condition. Printed in Japan
ALISO VIEJO, Calif., Oct. 4, 2016 /PRNewswire/ -- Microsemi Announces New Family of High Performance Frequency Synthesizers and Rate Converters. Leveraging...
The worlds first commercially available string synthesizer, the Eminent 310, came from an unlikely source, a Dutch home organ manufacturer. It was further advanced through an even more unlikely partnership, with the legendary American synth makers ARP...
List of all the English words with 24 letters beginning with O. octahydroxyanthraquinone, oligodeoxyribonucleotide, otorhinolaryngologically
Price: $4799.00; Manufacturer: Biotage; Item ID: 2029351; Warranty: 30-Day Money-Back Guarantee; Description: Biotage Initiator EXP
바이오닉스사는 1998년 Oligo synthesis service를 시작한 이래, 최신 설비와 노하우를 바탕으로 고품질 DNA Oligonucleotide를 생산/공급하고 있습니다. 특히 Global Oligo 제조공급사인 BIOBASIC®사로부터 지속적인 신규 장비와 기술을 도입하여 그 품질 향상을 도모하고 있으며, 이를 기반으로 업계 최고 수준의 품질을 자랑하는 Oligo와 Gene synthesis service 제공하고 있습니다 ...
Oligonucleotides have been researched for years and are becoming prevalent in todays society. The role of oligonucleotides in therapy has been increasing tenfold over the past ten years. This is […]. ...
Chemical synthesis of oligodeoxyribonucleotides by the hydrogenphosphonate approach". Tetrahedron Lett. 27 (34): 4051. doi: ... Letsinger, R. L.; Mahadevan, V. (1966). "Stepwise synthesis of oligodeoxyribonucleotides on an insoluble polymer support". J. ... doi:10.1016/s0040-4020(01)87958-8. Beaucage, S L. "Oligodeoxyribonucleotides synthesis. Phosphoramidite approach. Methods in ... "Cleavage of oligodeoxyribonucleotides from controlled-pore glass supports and their rapid deprotection by gaseous amines". ...
Her dissertation was titled The synthesis of oligodeoxyribonucleotides with RNA ligase. Her doctoral advisor was R.I. Gumport. ... Hinton, Deborah Meetze (1980). The synthesis of oligodeoxyribonucleotides with RNA ligase (Thesis). OCLC 7077827. "Principal ...
... and stereochemistry of phosphorothioate analogs of oligodeoxyribonucleotides". Journal of the American Chemical Society. 106 ( ...
"Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides". Gene. 164 (1): 49-53. doi: ...
With polynucleotides he has developed new methods for chemical and enzymatic synthesis of oligodeoxyribonucleotides; devised ... pioneered application of synthetic oligodeoxyribonucleotides to problems in molecular biology including their use as probes in ...
"Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides". Gene. 164 (1): 49-53. doi: ...
1995). "Single step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides". Gene. 164 (1): 49- ... 1995). "Single step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides". Gene. 164 (1): 49- ...
Toulmé JJ, Hélène C (December 1988). "Antimessenger oligodeoxyribonucleotides: an alternative to antisense RNA for artificial ...
"Hybridization of synthetic oligodeoxyribonucleotides to Phi-X 174 DNA: the effect of single base pair mismatch". Nucleic Acids ...
Preparation of ethylene glycol phosphate linked oligodeoxyribonucleotides as phospholipase A2 inhibitors. U.S. (1999), 39 pp., ...
October 2003). "Innate immune responses induced by CpG oligodeoxyribonucleotide stimulation of ovine blood mononuclear cells". ...
... with oligodeoxyribonucleotides". Biochem. Biophys. Res. Commun. 264 (2): 493-7. doi:10.1006/bbrc.1999.1566. PMID 10529391. ...
... simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping". Nucleic Acids Research. 1 (3): 331 ...
"A single anti-microRNA antisense oligodeoxyribonucleotide (AMO) targeting multiple microRNAs offers an improved approach for ...
"DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping" ...
O-ethyl oligoribonucleotides and phosphorothioate oligodeoxyribonucleotides as inhibitors of the in vitro U7 snRNP-dependent ...
1981). "The complete amino acid sequence of human fibroblast interferon as deduced using synthetic oligodeoxyribonucleotide ...
... deficiency causing congenital lipoid adrenal hyperplasia using bovine-sequence P450scc oligodeoxyribonucleotide probes". ...
... s are composed of 2'-deoxyribonucleotides (oligodeoxyribonucleotides), which can be modified at the backbone or ...
It is made of four double-stranded oligodeoxyribonucleotides that are attached to a carrier platform and are designed to block ...
Linkers are short, double stranded pieces of DNA (oligodeoxyribonucleotide) about 8 to 12 nucleotide pairs long that include a ...
... oligodeoxyribonucleotides, antisense MeSH D27.720.470.530.600.150.640 - oligonucleotides, antisense MeSH D27.720.470.530. ... 600.150.640.640 - oligodeoxyribonucleotides, antisense MeSH D27.720.470.530.600.150.640.645 - oligoribonucleotides, antisense ...
MeSH D13.150.200.640 - oligodeoxyribonucleotides, antisense MeSH D13.150.480.640 - oligodeoxyribonucleotides, antisense MeSH ... oligodeoxyribonucleotides, antisense MeSH D13.444.308.180 - dna, archaeal MeSH D13.444.308.212 - dna, bacterial MeSH D13.444. ... oligodeoxyribonucleotides MeSH D13.695.578.424.450.275 - dna primers MeSH D13.695.578.424.480 - oligonucleotides, antisense ... oligodeoxyribonucleotides, antisense MeSH D13.444.600.150.640 - oligonucleotides, antisense MeSH D13.444.600.150.640.640 - ...
... simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping". Nucleic Acids Research 1: 331-353 ...
"The complete amino acid sequence of human fibroblast interferon as deduced using synthetic oligodeoxyribonucleotide primers of ...
... is an antisense oligodeoxyribonucleotide being studied as a possible treatment for several types of cancer, including chronic ...
9. Lu Y, Xiao J, Lin H, Bai Y, Luo X, Wang Z, Yang B. A single anti-microRNA antisense oligodeoxyribonucleotide (AMO) targeting ...
... oligodeoxyribonucleotide - oligonucleotide - oncogene - oncovirus - open reading frame - operator - operon - origin of ...
Constructed mutants using synthetic oligodeoxyribonucleotides as site-specific mutagens. In: Genetic engineering. principles ...
Synthetic oligodeoxyribonucleotides (ODNs) containing unmethylated CpG recapitulate the activation of TLR9 by microbial DNA. ... Species-Specific Minimal Sequence Motif for Oligodeoxyribonucleotides Activating Mouse TLR9 J Immunol. 2015 Nov 1;195(9):4396- ... Synthetic oligodeoxyribonucleotides (ODNs) containing unmethylated CpG recapitulate the activation of TLR9 by microbial DNA. ...
DNA Cleavage by 111In-Labeled Oligodeoxyribonucleotides. Valeri N. Karamychev, Igor G. Panyutin, Meyong-Kon Kim, Nhat Le, Chang ... DNA Cleavage by 111In-Labeled Oligodeoxyribonucleotides. Valeri N. Karamychev, Igor G. Panyutin, Meyong-Kon Kim, Nhat Le, Chang ... DNA Cleavage by 111In-Labeled Oligodeoxyribonucleotides. Valeri N. Karamychev, Igor G. Panyutin, Meyong-Kon Kim, Nhat Le, Chang ... DNA Cleavage by 111In-Labeled Oligodeoxyribonucleotides Message Subject (Your Name) has sent you a message from Journal of ...
We examined the effects of transduction with high-affinity antisense oligodeoxyribonucleotides (ODNs) designed to target the ... Administration of dendritic cells transduced with antisense oligodeoxyribonucleotides targeting CD80 or CD86 prolongs allograft ...
oligodeoxyribonucleotide. pDC. plasmacytoid dendritic cell. PD. phosphodiester backbone. PTO. phosphorothioate. wt. wild-type. ... CpG oligodeoxyribonucleotides rescue mature spleen B cells from spontaneous apoptosis and promote cell cycle entry. J. Immunol. ... Species-Specific Minimal Sequence Motif for Oligodeoxyribonucleotides Activating Mouse TLR9. Jelka Pohar, Duško Lainšček, ... Species-Specific Minimal Sequence Motif for Oligodeoxyribonucleotides Activating Mouse TLR9. Jelka Pohar, Duško Lainšček, ...
Home › Online Presentations › Oligodeoxyribonucleotide Association with Single-Walled Carbon Nanotubes › About ... Jennifer McDonald (2007), "Oligodeoxyribonucleotide Association with Single-Walled Carbon Nanotubes," http://nanohub.org/ ...
Preparation of Oligodeoxyribonucleotides Containing the Pyrimidine(6-4)pyrimidone Photoproduct by Using a Dinucleotide Building ... oligodeoxyribonucleotides. Although this type of lesion is frequently found at thymine‐cytosine sites, the building block of ...
... ... liposomal formulation was synthesized and evaluated for the delivery of a phosphorothioate antisense oligodeoxyribonucleotide ( ...
Use of Oligo - Deoxyribonucleotides Containing Base Analogues to Study the Sequence Specificity of the Eco R1 Restriction ... Use of Oligo - Deoxyribonucleotides Containing Base Analogues to Study the Sequence Specificity of the Eco R1 Restriction ... Use of Oligo - Deoxyribonucleotides Containing Base Analogues to Study the Sequence Specificity of the Eco R1 Restriction ... Self-complementary oligodeoxyribonucleotides with base analogues in the EcoR1 recognition sequence were synthesized by a ...
... inhibition of cellular proliferation by antisense oligodeoxyribonucleotides ... inhibition of cellular proliferation by antisense oligodeoxyribonucleotides ... inhibition of cellular proliferation by antisense oligodeoxyribonucleotides ... inhibition of cellular proliferation by antisense oligodeoxyribonucleotides ...
This study investigated the co-delivery of plasmid DNA and antisense oligodeoxyribonucleotide (AS ODN) into carcinoma cells by ... BACKGROUND/AIM: This study investigated the co-delivery of plasmid DNA and antisense oligodeoxyribonucleotide (AS ODN) into ... Inhibition of Human Carcinoma Cell Growth via Co-Delivery of p53 Plasmid DNA and bcl-2 Antisense Oligodeoxyribonucleotide by ...
... of 8-histaminyl deoxyadenosine has been prepared and successfully incorporated into a short oligodeoxyribonucleotide. The ... into oligodeoxyribonucleotides by solid phase phosphoramidite coupling. ... into oligodeoxyribonucleotides by solid phase phosphoramidite coupling. ... into oligodeoxyribonucleotides by solid phase phosphoramidite coupling ...
Oligo- ?-deoxyribonucleotides and oligo- ?-deoxyribonucleotides involving 2-aminopurine and guanine for triple-helix formation ... Partially phosphate-methylated oligodeoxyribonucleotides have been synthesized on an oxalyl-CPG derivatized support using an ... Oligo- ?-deoxyribonucleotides with a modified nucleic base and covalently-linked to reactive agents ... Synthesis and physicochemical studies of partially phosphate-methylated oligodeoxyribonucleotides. * Synthesis of an ...
Such oligodeoxyribonucleotides could form the basis for the design of improved AGT inhibitors. AGT and mutants P140A and G156A ... Such oligodeoxyribonucleotides could form the basis for the design of improved AGT inhibitors. AGT and mutants P140A and G156A ... Such oligodeoxyribonucleotides could form the basis for the design of improved AGT inhibitors. AGT and mutants P140A and G156A ... Such oligodeoxyribonucleotides could form the basis for the design of improved AGT inhibitors. AGT and mutants P140A and G156A ...
Controlled loading of oligodeoxyribonucleotide monolayers onto unoxidized crystalline silicon; fluorescence-based determination ... Controlled loading of oligodeoxyribonucleotide monolayers onto unoxidized crystalline silicon; fluorescence-based determination ... article{Cattaruzza2006ControlledLO, title={Controlled loading of oligodeoxyribonucleotide monolayers onto unoxidized ...
oligodeoxyribonucleotides, etc ; alkynes; chemical reactions; crosslinking; nucleic acids; nucleosides; Show all 6 Subjects. ... oligodeoxyribonucleotides, etc ; Toll-like receptor 9; adjuvants; amino acids; humans; immune response; mice; open reading ... oligodeoxyribonucleotides, etc ; Toll-like receptor 2; Toll-like receptor 4; Toll-like receptor 7; adjuvants; antigens; ... oligodeoxyribonucleotides, etc ; Toll-like receptor 9; adjuvants; animal models; diploidy; disease control; dogs; humans; ...
You searched for: Subject oligodeoxyribonucleotides Remove constraint Subject: oligodeoxyribonucleotides Journal Vaccine ... oligodeoxyribonucleotides, etc ; Toll-like receptor 9; agonists; antigens; blood serum; cell-mediated immunity; encapsulation; ... oligodeoxyribonucleotides, etc ; Escherichia coli; Gram-negative bacteria; Toll-like receptor 9; adjuvants; adults; adverse ... oligodeoxyribonucleotides, etc ; Macaca fascicularis; T-lymphocytes; antibodies; beta-glucans; dendritic cells; drug delivery ...
Taken together, these studies demonstrate that a three-dimensional surface may enable use of shorter oligodeoxyribonucleotide ... However, the array fabrication method, hybridization conditions, and oligodeoxyribonucleotide probe length can impact the ... of the oligodeoxyribonucleotide is available for hybridization. Kinetic parameters were also investigated. As anticipated, ... For example, oligodeoxyribonucleotide probes can be covalently attached to a surface [10], synthesized in situ [11-13], or ...
Oligodeoxyribonucleotides Comprising O6-Benzylguanine and Their Use Robert Moschel et al. (NCI) ... For example, oligodeoxyribonucleotides between 7 and 11 nucleotides in length containing multiple O6-benzylguanines are ... In addition, oligodeoxyribonucleotides containing O6-benzylguanine are effective in activating several mutant alkyltransferase ... Therefore, oligodeoxyribonucleotides containing multiple O6-benzylguanine residues may be more effective chemotherapy adjuvants ...
Triplet repeat sequences in human DNA can be detected by hybridization to a synthetic (5-CGG-3)17 oligodeoxyribonucleotide. * ... We demonstrate here that a synthetic (CGG)17 oligodeoxyribonucleotide can be utilized as hybridization probe to visualize some ... Oligodeoxyribonucleotides; Phosphorus Radioisotopes; Repetitive Sequences, Nucleic Acid; Restriction Mapping Main Research Area ...
Oligodeoxyribonucleotides. LinkOut - more resources. Other Literature Sources. *Cited by Patents in - The Lens ...
oligodeoxyribonucleotides;. DNG,. deoxynucleic guanidine;. Rt,. retention time;. MMTr,. monomethoxytrityl. *Accepted July 27, ... The nuclease resistance of the oligodeoxyribonucleotides capped with guanidinium linkages at 5′ and 3′ ends are reported. The ... The use of antisense oligodeoxyribonucleotides (ODNs) to regulate gene products requires the development of modified ODNs ...
Antisense oligodeoxyribonucleotides. In a preferred embodiment of the present invention, a PS-ODN is used which has the dual ... In a preferred embodiment, the present invention includes the use of phosphorothioate oligodeoxyribonucleotides ("PS-ODNs") in ... Westermann, "Inhibition of Expression of SV40 Virus Large T-Antigen by Antisense Oligodeoxyribonucleotides", Biomed.Biochim. ... Westermann, Inhibition of Expression of SV40 Virus Large T Antigen by Antisense Oligodeoxyribonucleotides , Biomed.Biochim.Acta ...
Oligodeoxyribonucleotides Grant support * R01 CA032685/CA/NCI NIH HHS/United States * R01 CA087025/CA/NCI NIH HHS/United States ...
Oligodeoxyribonucleotides. *RNA/*chemistry/metabolism. *Recombinant Proteins/chemistry/metabolism. *Support, Non-U.S. Govt ...
3. Beaucage, "Oligodeoxyribonucleotides synthesis. Phosphoramidite approach", Methods Mol. Biol. 20:33-61, 1993. ... Beaucage, S.L. (1993). "Oligodeoxyribonucleotides Synthesis. Phosphoramidite Approach," Chapter 3 in Methods in Molecular ...
Chiu, S. J., Marcucci, G., & Lee, R. J. (2006). Efficient delivery of an antisense oligodeoxyribonucleotide formulated in ... Chiu, Shih Jiuan ; Marcucci, Guido ; Lee, Robert J. / Efficient delivery of an antisense oligodeoxyribonucleotide formulated in ... Fingerprint Dive into the research topics of Efficient delivery of an antisense oligodeoxyribonucleotide formulated in folate ... Chiu, SJ, Marcucci, G & Lee, RJ 2006, Efficient delivery of an antisense oligodeoxyribonucleotide formulated in folate ...
Oligodeoxyribonucleotides were purchased from Metabion. The 5′ ends of oligonucleotides were radiolabeled using T4 ...
oligodeoxyribonucleotide(s). NER. nucleotide excision repair. dCFAB. 5-[3-(4-azido-2,3,5,6-tetrafluorobenzamido)propoxyprop-1- ...
Pharmacokinetics of antisense oligodeoxyribonucleotides (cyclin B1 and CDC 2 kinase) in the vessel wall in vivo: enhanced ... Pharmacokinetics of antisense oligodeoxyribonucleotides (cyclin B1 and CDC 2 kinase) in the vessel wall in vivo: enhanced ... we examined the cellular fate of antisense oligodeoxyribonucleotides (oligos) in the vessel wall in vivo. Direct transfer of ...
The oligodeoxyribonucleotide ethyl phosphotriesters d-Tp(Et)Gp(Et)G and d-Tp(Et)Tp(Et)Cp(Et)A, which are complementary to the 3 ... N2 - The oligodeoxyribonucleotide ethyl phosphotriesters d-Tp(Et)Gp(Et)G and d-Tp(Et)Tp(Et)Cp(Et)A, which are complementary to ... AB - The oligodeoxyribonucleotide ethyl phosphotriesters d-Tp(Et)Gp(Et)G and d-Tp(Et)Tp(Et)Cp(Et)A, which are complementary to ... abstract = "The oligodeoxyribonucleotide ethyl phosphotriesters d-Tp(Et)Gp(Et)G and d-Tp(Et)Tp(Et)Cp(Et)A, which are ...
  • A novel transferrin receptor (TfR)-targeted liposomal formulation was synthesized and evaluated for the delivery of a phosphorothioate antisense oligodeoxyribonucleotide (ODN) (G3139, oblimerson sodium, or Genasense™) to Bcl-2 in K562 leukemia cells. (ovid.com)
  • Synergistic Inhibition of Human Carcinoma Cell Growth via Co-Delivery of p53 Plasmid DNA and bcl-2 Antisense Oligodeoxyribonucleotide by Cholic Acid-modified Polyethylenimine. (osu.edu)
  • This study investigated the co-delivery of plasmid DNA and antisense oligodeoxyribonucleotide (AS ODN) into carcinoma cells by cholic acid-modified polyethylenimine (PEI-CA). (osu.edu)
  • The aim of this study was to develop an antisense oligodeoxyribonucleotide (ODN) carrier targeting FR-overexpressing cancer cells using folate (FA) as the targeting moiety. (elsevier.com)
  • Lee, Robert J. / Efficient delivery of an antisense oligodeoxyribonucleotide formulated in folate receptor-targeted liposomes . (elsevier.com)
  • In addition, the antisense oligodeoxyribonucleotide to MnSOD significantly inhibited both LIF and caSTAT3-mediated protective effects. (ahajournals.org)
  • These mutations had a much smaller effect on the reaction with O 6 -benzylguanine when it was incorporated into a short single-stranded oligodeoxyribonucleotide. (elsevier.com)
  • ssODN, single-stranded oligodeoxyribonucleotide. (jci.org)
  • Synthetic oligodeoxyribonucleotides (ODNs) containing unmethylated CpG recapitulate the activation of TLR9 by microbial DNA. (nih.gov)
  • We examined the effects of transduction with high-affinity antisense oligodeoxyribonucleotides (ODNs) designed to target the mRNA of CD80 or CD86 on the phenotype and function of dendritic cells (DCs). (nih.gov)
  • single stranded DNA with unmethylated CpG motifs, characteristic of the genomes of bacteria and viruses, and oligodeoxyribonucleotides (ODNs) containing stimulatory CpG motifs activate the innate immune response ( 1 ). (jimmunol.org)
  • The use of antisense oligodeoxyribonucleotides (ODNs) to regulate gene products requires the development of modified ODNs possessing the properties of enhanced cellular uptake, nuclease resistance, and sequence specific hybridization to complementary RNAs. (pnas.org)
  • In a preferred embodiment, the present invention includes the use of phosphorothioate oligodeoxyribonucleotides ("PS-ODNs") in such methods. (google.com)
  • Her dissertation was titled The synthesis of oligodeoxyribonucleotides with RNA ligase. (wikipedia.org)
  • The synthesis of oligodeoxyribonucleotides with RNA ligase (Thesis). (wikipedia.org)
  • For oligonucleotides see: Letsinger, R.L. and Mahadevan, V., Stepwise synthesis of oligodeoxyribonucleotides on an insoluble polymer support , J. Am. Chem. (springer.com)
  • To study these proteins in the Gram + pathogen, Staphylococcus aureus, two pairs of degenerate oligodeoxyribonucleotides (oligos) that corresponded to conserved sequences contained within sensor protein-encoding genes were synthesized. (nebraska.edu)
  • Random mutagenesis using degenerate oligodeoxyribonucleotides. (semanticscholar.org)
  • We demonstrate here that a synthetic (CGG)17 oligodeoxyribonucleotide can be utilized as hybridization probe to visualize some of the triplet repeats in the human genome. (forskningsdatabasen.dk)
  • Synthetic oligodeoxyribonucleotide probes to detect Kanagawa phenomenon-positive Vibrio parahaemolyticus. (asm.org)
  • Using a highly efficient viral HVJ (hemagglutinating virus of Japan) liposome-mediated transfer method, we examined the cellular fate of antisense oligodeoxyribonucleotides (oligos) in the vessel wall in vivo. (duke.edu)
  • Treatment with antisense oligodeoxyribonucleotides targeting Fas mRNA attenuates renal ischemia-reperfusion injury. (genomenewsnetwork.org)
  • Administration of dendritic cells transduced with antisense oligodeoxyribonucleotides targeting CD80 or CD86 prolongs allograft survival. (nih.gov)
  • Bayles, KW 1993, ' The use of degenerate, sensor gene-specific, oligodeoxyribonucleotide primers to amplify DNA fragments from Staphylococcus aureus ', Gene , vol. 123, no. 1, pp. 99-103. (nebraska.edu)
  • As oligodeoxyribonucleotide 2114-treated MRL lpr/lpr mice were not exposed to exogenous CpG-DNA, these effects should relate to a blockade of CpG motifs in endogenous DNA. (asnjournals.org)
  • However, repetitive injections of CpG-oligodeoxyribonucleotides (ODN) cause inappropriate lymphoproliferation in mice ( 3 ). (asnjournals.org)
  • The effects of mismatches in the probes attached to the microarray also demonstrate that most, if not all, of the oligodeoxyribonucleotide is available for hybridization. (biomedcentral.com)
  • Taken together, these studies demonstrate that a three-dimensional surface may enable use of shorter oligodeoxyribonucleotide probes and that hybridization time may be critical in improving the accuracy of microarray data. (biomedcentral.com)
  • The oligodeoxyribonucleotide ethyl phosphotriesters d-Tp(Et)Gp(Et)G and d-Tp(Et)Tp(Et)Cp(Et)A, which are complementary to the 3′-CpCpA terminus and -UpGpApA- anticodon region, respectively, of tRNA phe coli have been used as in vitro probes of the structure and function of tRNA. (elsevier.com)
  • The nuclease resistance of the oligodeoxyribonucleotides capped with guanidinium linkages at 5′ and 3′ ends are reported. (pnas.org)
  • However, the array fabrication method, hybridization conditions, and oligodeoxyribonucleotide probe length can impact the performance of a DNA microarray platform. (biomedcentral.com)
  • Although PCR preparation is not a factor in the fabrication of oligodeoxyribonucleotide arrays, a similar problem exists, namely, surface probe density. (biomedcentral.com)
  • Preparation of ethylene glycol phosphate linked oligodeoxyribonucleotides as phospholipase A2 inhibitors. (wikipedia.org)
  • The great difference between these techniques is related to the proteases involved in the process, while for interference RNA the RISC machinery acts, for antisense oligodeoxyribonucleotides RNase H cleaves the RNA in the duplex DNA-RNA. (usp.br)
  • Maskos, U. and Southern, E.M. (1992) Parallel analysis of oligodeoxyribonucleotide (oligonucleotide) interactions. (scirp.org)
  • The 3' phosphoramidite of 8-histaminyl deoxyadenosine has been prepared and successfully incorporated into a short oligodeoxyribonucleotide. (ubc.ca)
  • In addition, the recognition sequence is short and self-complementary oligodeoxyribonucleotides that are only eight base pairs long and contain the recognition sequence are substrates for the enzymes thereby allowing simple substrates and their analogues to be examined. (illinois.edu)
  • Changes in the AGT active site pocket can therefore affect the preference for repair of O 6 -benzyl or - methyl groups when present in an oligodeoxyribonucleotide without altering the reaction with free O 6 -benzylguanine. (elsevier.com)
  • Pharmacokinetics of antisense oligodeoxyribonucleotides (cyclin B1 and CDC 2 kinase) in the vessel wall in vivo: enhanced therapeutic utility for restenosis by HVJ-liposome delivery. (duke.edu)
  • Such oligodeoxyribonucleotides could form the basis for the design of improved AGT inhibitors. (elsevier.com)
  • Partially phosphate-methylated oligodeoxyribonucleotides have been synthesized on an oxalyl-CPG derivatized support using an isopropoxyacetyl group for the protection of the exocyclic amine of the nucleic bases. (cnrs-orleans.fr)
  • Self-complementary oligodeoxyribonucleotides with base analogues in the EcoR1 recognition sequence were synthesized by a general method that allows incorporation of the analogues at specific positions in the sequence. (illinois.edu)