Suture Techniques
Caprolactam
Polydioxanone
Aminocaproates
Enzymes, Immobilized
Flavobacterium
Aminocaproic Acid
Filtration
DNA Probes
Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.
Polyglactin 910
Surgical Staplers
Surgical Wound Dehiscence
Nucleic Acid Hybridization
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
T-Lymphocytes
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Tissue Adhesives
Cyanoacrylates
Nylons
Abdominal Wall
The outer margins of the ABDOMEN, extending from the osteocartilaginous thoracic cage to the PELVIS. Though its major part is muscular, the abdominal wall consists of at least seven layers: the SKIN, subcutaneous fat, deep FASCIA; ABDOMINAL MUSCLES, transversalis fascia, extraperitoneal fat, and the parietal PERITONEUM.
Plastics
Polymeric materials (usually organic) of large molecular weight which can be shaped by flow. Plastic usually refers to the final product with fillers, plasticizers, pigments, and stabilizers included (versus the resin, the homogeneous polymeric starting material). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Polyethylene Terephthalates
Concanavalin A
Rosette Formation
The in vitro formation of clusters consisting of a cell (usually a lymphocyte) surrounded by antigenic cells or antigen-bearing particles (usually erythrocytes, which may or may not be coated with antibody or antibody and complement). The rosette-forming cell may be an antibody-forming cell, a memory cell, a T-cell, a cell bearing surface cytophilic antibodies, or a monocyte possessing Fc receptors. Rosette formation can be used to identify specific populations of these cells.
Lymphocytes
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Glutaral
Specimen Handling
Rumen
The first stomach of ruminants. It lies on the left side of the body, occupying the whole of the left side of the abdomen and even stretching across the median plane of the body to the right side. It is capacious, divided into an upper and a lower sac, each of which has a blind sac at its posterior extremity. The rumen is lined by mucous membrane containing no digestive glands, but mucus-secreting glands are present in large numbers. Coarse, partially chewed food is stored and churned in the rumen until the animal finds circumstances convenient for rumination. When this occurs, little balls of food are regurgitated through the esophagus into the mouth, and are subjected to a second more thorough mastication, swallowed, and passed on into other parts of the compound stomach. (From Black's Veterinary Dictionary, 17th ed)
Polyethylene
A vinyl polymer made from ethylene. It can be branched or linear. Branched or low-density polyethylene is tough and pliable but not to the same degree as linear polyethylene. Linear or high-density polyethylene has a greater hardness and tensile strength. Polyethylene is used in a variety of products, including implants and prostheses.
Oligonucleotide Array Sequence Analysis
Plasmapheresis
Glucose Oxidase
An enzyme of the oxidoreductase class that catalyzes the conversion of beta-D-glucose and oxygen to D-glucono-1,5-lactone and peroxide. It is a flavoprotein, highly specific for beta-D-glucose. The enzyme is produced by Penicillium notatum and other fungi and has antibacterial activity in the presence of glucose and oxygen. It is used to estimate glucose concentration in blood or urine samples through the formation of colored dyes by the hydrogen peroxide produced in the reaction. (From Enzyme Nomenclature, 1992) EC 1.1.3.4.
B-Lymphocytes
Lymphocyte Activation
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
Membranes, Artificial
Oligonucleotide Probes
Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.
Tensile Strength
Poaceae
Astigmatism
Unequal curvature of the refractive surfaces of the eye. Thus a point source of light cannot be brought to a point focus on the retina but is spread over a more or less diffuse area. This results from the radius of curvature in one plane being longer or shorter than the radius at right angles to it. (Dorland, 27th ed)
Cytotoxicity, Immunologic
Granulocytes
Biodegradation, Environmental
Cytotoxicity Tests, Immunologic
Leukocytes
Neutrophils
Gene Expression Profiling
Cornea
The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)
Antilymphocyte Serum
Cattle
Chemotactic Factors
Immunosuppression
Deliberate prevention or diminution of the host's immune response. It may be nonspecific as in the administration of immunosuppressive agents (drugs or radiation) or by lymphocyte depletion or may be specific as in desensitization or the simultaneous administration of antigen and immunosuppressive drugs.
Lymphocyte Culture Test, Mixed
Measure of histocompatibility at the HL-A locus. Peripheral blood lymphocytes from two individuals are mixed together in tissue culture for several days. Lymphocytes from incompatible individuals will stimulate each other to proliferate significantly (measured by tritiated thymidine uptake) whereas those from compatible individuals will not. In the one-way MLC test, the lymphocytes from one of the individuals are inactivated (usually by treatment with MITOMYCIN or radiation) thereby allowing only the untreated remaining population of cells to proliferate in response to foreign histocompatibility antigens.
Dose-Response Relationship, Immunologic
Anopheles gambiae
Antigens, Thy-1
Immunization, Passive
Microscopy, Electron, Scanning
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
Lectins
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
Killer Cells, Natural
Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.
Polymers
Mice, Inbred Strains
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
Mitogens
Isoantigens
Cells, Cultured
Mosquito Control
Macrophages
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Immunity, Cellular
Gene Library
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Base Sequence
Hypersensitivity, Delayed
Dietary Proteins
Luminescent Measurements
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Arterial Occlusive Diseases
Catheterization
Leukocyte Count
Materials Testing
Sensitivity and Specificity
Antigens, Surface
T-Lymphocytes, Regulatory
Surface Properties
Chemotaxis, Leukocyte
Serum Albumin, Bovine
DNA
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Rabbits
Random Allocation
Phagocytosis
Indomethacin
Thymus Gland
A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.
Sheep
Rats, Wistar
Antibody Formation
Disease Models, Animal
Reproducibility of Results
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
Plasmids
Lymph Nodes
Rats, Inbred Strains
Temperature
Culture Media
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Blotting, Southern
Monocytes
Immune Tolerance
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
Swine
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Polymorphism, Restriction Fragment Length
DNA, Complementary
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Postoperative Complications
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Species Specificity
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Immunoglobulin G
Gene Expression
Brain
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
Interleukin-10 and antigen-presenting cells actively suppress Th1 cells in BALB/c mice infected with the filarial parasite Brugia pahangi. (1/451)
Infection with the third-stage larvae (L3) of the filarial nematode Brugia results in a Th2-biased immune response in mice and humans. Previously we have shown that the production of interleukin 4 (IL-4) is critical for down-regulating polyclonal Th1 responses in L3-infected mice. However, the in vitro neutralization of IL-4 did not fully recover the defective polyclonal Th1 responses, nor did it result in the production of any antigen (Ag)-specific Th1 cytokines, suggesting that perhaps infection with L3 does not result in priming of Th1 cells in vivo. In this study, we analyzed the role of IL-10 and Ag-presenting cells (APCs) in the spleen as additional factors controlling the Th2 bias in infected mice. Our data show that IL-10 and APCs also contribute to the suppression of mitogen-driven Th1 responses of spleen cells from infected mice. In addition, the neutralization of IL-10 or the replacement of the resident APC population from spleen cell cultures resulted in the production of Ag-specific Th1 cytokines. Irradiated spleen cells from either L3-infected or uninfected mice were able to restore Ag-specific Th1 responses in vitro. Therefore, it appears that Brugia-reactive Th1 cells are primed following infection with L3, but are actively suppressed in vivo by a mechanism that involves IL-10 and the resident APC population, but not IL-4. These results indicate that a complex interplay of cytokines and cell populations underscores the Th2-polarized response in L3-infected mice. (+info)Inhibition of Ets-1 DNA binding and ternary complex formation between Ets-1, NF-kappaB, and DNA by a designed DNA-binding ligand. (2/451)
Sequence-specific pyrrole-imidazole polyamides can be designed to interfere with transcription factor binding and to regulate gene expression, both in vitro and in living cells. Polyamides bound adjacent to the recognition sites for TBP, Ets-1, and LEF-1 in the human immunodeficiency virus, type 1 (HIV-1), long terminal repeat inhibited transcription in cell-free assays and viral replication in human peripheral blood lymphocytes. The DNA binding activity of the transcription factor Ets-1 is specifically inhibited by a polyamide bound in the minor groove. Ets-1 is a member of the winged-helix-turn-helix family of transcription factors and binds DNA through a recognition helix bound in the major groove with additional phosphate contacts on either side of this major groove interaction. The inhibitory polyamide possibly interferes with phosphate contacts made by Ets-1, by occupying the adjacent minor groove. Full-length Ets-1 binds the HIV-1 enhancer through cooperative interactions with the p50 subunit of NF-kappaB, and the Ets-inhibitory polyamide also blocks formation of ternary Ets-1. NF-kappaB.DNA complexes on the HIV-1 enhancer. A polyamide bound adjacent to the recognition site for NF-kappaB also inhibits NF-kappaB binding and ternary complex formation. These results broaden the application range of minor groove-binding polyamides and demonstrate that these DNA ligands are powerful inhibitors of DNA-binding proteins that predominantly use major groove contacts and of cooperative protein-DNA ternary complexes. (+info)Sensitivity issues in DNA array-based expression measurements and performance of nylon microarrays for small samples. (3/451)
DNA or oligonucleotide arrays are widely used for large-scale expression measurements, using various implementations: macroarrays in which DNA is spotted onto nylon membranes of relatively large dimensions (with radioactive detection) on the one hand; microarrays on glass slides and oligonucleotide chips, both used with fluorescent probes, on the other hand. Nylon micro-arrays with colourimetric detection have also been described recently. The small physical dimensions of miniaturized systems allow small hybridization volumes (2-100 microl) and provide high probe concentrations, in contrast to macroarrays. We show, however, that actual sensitivity (defined as the amount of sample necessary for detection of a given mRNA species) is in fact similar for all these systems and that this is mostly due to the very different amounts of target material present on the respective arrays. We then demonstrate that the combination of nylon microarrays with(33)P-labelled radioactive probes provides 100-fold better sensitivity, making it possible to perform expression profiling experiments using submicrogram amounts of unamplified total RNA from small biological samples. This has important implications in basic and clinical research and makes this alternative approach particularly suitable for groups operating in an academic context. (+info)Functional and morphologic characteristics of the leukemic cells of a patient with acute monocytic leukemia: correlation with clinical features. (4/451)
The clinical course of a patient with acute monocytic leukemia and prominent infiltration of the skin and testes is described. In vitro studies demonstrated that the circulating monocyte precursors were capable of adherence to nylon fibers, and phagocytosis of bacteria and latex particles. In vivo, migration of leukemic cells to skin windows was observed. Extreme nuclear folding, marked surface activity, and morphologic features suggesting nuclear and cytoplasmic maturation were seen by light and electron microscopy. The presence of morphologically and functionally more differentiated monocytic cells may account for the marked tiuuse invasion in this patient and, possibly, in other patients with monocytic leukemia. (+info)In vitro cytotoxicity of textile paint components linked to the "Ardystil syndrome". (5/451)
The spraying of a paint formula (Acramin F system) had led to severe pulmonary disease in textile printing sprayers in Spain and Algeria (Ardystil syndrome). In order to elucidate the underlying mechanisms of the toxicity of this paint and its main polymeric components, Acramin FWR, Acramin FWN, Acrafix FHN, and Acramoll W, we have undertaken studies using a battery of different cell-types and assessing in vitro cytotoxicity by measuring LDH leakage. This study shows that, as in in vivo studies, the three polycationic paint components, Acramin FWR (a polyurea), Acramin FWN (a polyamide-amine), and Acrafix FHN (a polyamine) exhibited considerable cytotoxicity (LC50 generally below 100 microg/ml for an incubation of 20-24 h) in vitro, while Acramoll W, which is not a polycation, was almost non-toxic (in the concentration range tested). The cytotoxicity was comparable in primary cultures of rat and human type II pneumocytes and alveolar macrophages as well as in the pulmonary cell line A549 and the hepatic cell line HepG2. In human erythrocytes, the toxicity was less pronounced. We speculate that the multiple positive charges play an important role in the toxic mechanism. It is concluded that Acramin FWR and Acramin FWN have similar intrinsic toxicity and that these polymeric compounds, which have no irritant properties or systemic toxicity when given orally, exert a high, unexpected, degree of cytotoxicity. (+info)Local anaesthetic effect of topical amethocaine gel in neonates: randomised controlled trial. (6/451)
AIM: To assess the efficacy of amethocaine as a topical local anaesthetic in neonates. METHODS: A randomised, double blind controlled trial compared 4% amethocaine gel (Ametop) with placebo in 60 healthy neonates (29 to 42 weeks of gestation) in the first week after birth. Either 1.5 g 4% w/w amethocaine (gel) or 1.5 g placebo gel were applied to the dorsum of one foot. No gel was applied to the other foot. Each foot was occluded and left for one hour. Local anaesthesia was then assessed by eliciting the cutaneous withdrawal reflex in response to stimulation with a series of graded nylon filaments (von Frey hairs). The reflex was first elicited from the control and then the treated foot. The difference in filament thickness and deforming weight required to elicit the reflex was recorded. RESULTS: In infants treated with amethocaine, 17 of 31 (54. 8%) showed evidence of local anaesthetic action compared with five of 29 (17.2%) in the placebo group (p=0.003). The mean difference in deforming weight required to elicit the reflex was 18.8 g in the amethocaine group compared with 3.9 g in the placebo group (p=0.02). The apparent local anaesthetic action of the placebo can be explained by habituation to repeated stimulation. CONCLUSIONS: It is concluded that topical amethocaine gel has a local anaesthetic action on neonatal skin which merits further investigation. An effective and safe surface local anaesthetic would be valuable for the relief of procedure related pain in neonates. (+info)In-source decay of hyperbranched polyesteramides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. (7/451)
Hyperbranched polyesteramides (DA2), prepared from hexahydrophthalic anhydride (D) and diisopropanolamine (A) have been characterized, by use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), field desorption (FD)-MS, and electrospray ionization (ESI)-MS. MALDI of polyesteramides produces protonated molecules. The spectra show a complex chemical composition distribution and end-group distribution which are mainly composed of two series of homologous oligomers DnA(n)+1 - mH2O and DnA(n) - mH2O, where m = 1-2. Signals from protonated molecules DnAn+1 and DnAn are almost absent in the MALDI spectrum, whereas these ions are responsible for the base peak of DnA(n)+1 - mH2O and DnA(n) - mH2O (m = 1-2) clusters in the ESI spectrum. The absence of -OH end-groups signals in the MALDI spectrum is due to a metastable decay of protonated DnA(n)+1 and DnAn ions in the ion source of the MALDI mass spectrometer prior to ion extraction. In-source decay results in the formation of protonated lower DnA(n)+1 - mH2O and DnA(n) - mH2O oligomers and their corresponding neutrals, leading to wrong conclusions concerning the relative end-group distribution as a function of the degree of polymerization and the chemical composition. (+info)Sequence specific alkylation of DNA by hairpin pyrrole-imidazole polyamide conjugates. (8/451)
BACKGROUND: Pyrrole-imidazole polyamides are synthetic ligands that recognize predetermined sequences in the minor groove of DNA with affinities and specificities comparable to those of DNA-binding proteins. As a result of their DNA-binding properties, polyamides could deliver reactive moieties for covalent reaction at specific DNA sequences and thereby inhibit DNA-protein interactions. Site-specific alkylation of DNA could be a useful tool for regulating gene expression. As a minimal first step, we set out to design and synthesize a class of hairpin polyamides equipped with DNA alkylating agents and characterize the specificity and yield of covalent modification. RESULTS: Bis(dichloroethylamino)benzene derivatives of the well-characterized chlorambucil (CHL) were attached to the gamma turn of an eight-ring hairpin polyamide targeted to the HIV-1 promoter. We found that a hairpin polyamide-CHL conjugate binds and selectively alkylates predetermined sites in the HIV promoter at subnanomolar concentrations. Cleavage sites were determined on both strands of a restriction fragment containing the HIV-1 promoter, revealing good specificity and a high yield of alkylation. CONCLUSIONS: The ability of polyamide-CHL conjugates to sequence specifically alkylate double-stranded DNA in high yield and at low concentrations sets the stage for testing their use as regulators of gene expression in cell culture and ultimately in complex organisms. (+info)CiNii Articles - Comparative analysis of DNA alkylation by conjugates between pyrrole-imidazole hairpin polyamides...
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Modification of a polyamide reverse osmosis membrane by heat treatment for enhanced fouling resistance | Water Supply | IWA...
119050020 - DURATOOL - Machine Screw, M5, Nylon 6.6 (Polyamide 6.6), 20 mm, Cheese Head Slotted | Farnell element14
Moen Fingertip Bandages
Patent US5061763 - Stain resistant treatment for polyamide fibers - Google Patents
Indian Patents. 215443:A METHOD OF MASS-COLOURING POLYAMIDE MATERIAL AND A POLYAMIDE MATERIAL COLOURED THEREBY
Tech Lite Short Sleeve Low Crewe PCT Sketchbook - Icebreaker (UK)
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The Nylons
... discography at Discogs The Nylons at IMDb Article at canadianbands.com Article at thecanadianencyclopedia.ca ( ... The Nylons are an a cappella group founded in 1978 in Toronto, Ontario, Canada, best known for their covers of pop songs such ... The Nylons appeared in Season 3 of the show on the "Treasure Island" episode, singing "The Lion Sleeps Tonight". These songs ... The Nylons' original lineup consisted of Claude Morrison (tenor), Paul Cooper (baritone; born James Paul Cooper in Pikeville, ...
The Nylons (album)
The Nylons is the debut album from the Canadian a cappella quartet The Nylons released on Attic Records. The platinum-selling ... The Nylons at AllMusic v t e (Use Canadian English from January 2018, All Wikipedia articles written in Canadian English, Use ... The Nylons Marc Connors - tenor vocals Claude Morrison - tenor vocals Paul Cooper - baritone vocals Arnold Robinson - bass ...
Nylon
Incinerating nylons to recover the high energy used to create them is usually expensive, so most nylons reach the garbage dumps ... Most nylons are made from the reaction of a dicarboxylic acid with a diamine (e.g. PA66) or a lactam or amino acid with itself ... Additionally, nylons can be synthesized from diols and dinitriles using this method as well. It is easy to make mixtures of the ... Various nylons break down in fire and form hazardous smoke, and toxic fumes or ash, typically containing hydrogen cyanide. ...
Holly Randall
Randall has four photo books, Erotic Dream Girls, Kinky Nylons, Kinky Super Beauties and Kinky Lingerie published by Goliath ... Randall, Holly (2011). Kinky nylons. Frankfurt am Main: Goliath. ISBN 9783936709506. Wikimedia Commons has media related to ...
Canada Dry
"Nylon Studios Creates Music for New Canada Dry TV Ad". Nylons Studios. Retrieved February 21, 2015. Robertson, Heather (1995). ...
Denis Simpson
He was also an original member of the singing group The Nylons. Born in St. Ann's Bay, Jamaica, Simpson arrived in Canada at ... "Biography: The Nylons". AMG. Retrieved 7 May 2010. Denis Simpson at IMDb (Articles with short description, Short description is ... The original bass vocalist for The Nylons in 1978-79, he left the band to appear in the Broadway musical Indigo before they ... The Nylons members, Black Canadian LGBT people, 20th-century LGBT people, 21st-century LGBT people). ...
Crambe oil
... plastics and nylons. Erucic acid is traditionally derived from older varieties of rapeseed. Crambe oil is considered to be a ...
This Island Earth
Nylons - This Island Earth. January 2, 2012. Archived from the original on December 22, 2021 - via YouTube. Millikin, Eric. " ... The album Happy Together (1987) by the a cappella group The Nylons featured a track titled "This Island Earth". The video game ...
Condensation polymer
Examples include nylons and proteins. When prepared from amino-carboxylic acids, e.g. amino acids, the stoichiometry of the ...
Vincent M. Ward
"Nylons returns for one show only". lawattstimes.com. Retrieved 16 January 2014. "Nylons on stage one weekend only". Sentinel. ... Ward played the role of Kenzo in the play "Nylons" by Brandi Burks in Los Angeles in 2011. In 2015 Ward performed in The ...
I'm the Man (album)
ISBN 978-0-85712-595-8. Sexton, Paul (13 October 1979). "No Nylons but a Tasty Album". Record Mirror. p. 16. Coleman, Mark; ...
Billy Newton-Davis
Newton-Davis subsequently joined The Nylons in 1991. Since leaving the Nylons, he has primarily concentrated on songwriting and ... The Nylons members, Canadian male jazz musicians, 20th-century LGBT people, 21st-century LGBT people, Canadian male singer- ...
Anita De Bauch
"Example of appearance in Sheer Nylons online catalog". Sheernylons.co.uk. Archived from the original on 11 March 2012. ...
Cyclohexylbenzene
Cyclohexanone is an important precursor to some nylons. Qiao, Kun; Yokoyama, Chiaki (2004). "Novel Acidic Ionic Liquids ...
Utah Minute Women
She saved old nylons with runs in them. Very little trash went out of our home because everything was used two or three times ...
Cumene process
Cyclohexanone is an important precursor to some nylons. Starting with the alkylation of benzene with mixture of 1 and 2-butenes ...
Phenol
Cyclohexanone is an important precursor to some nylons. The direct oxidation of benzene (C6H6) to phenol is theoretically ...
Polyamide
Nylons must specifically include a straight chain (aliphatic) monomer. The amide link is produced from an amine group ( ... made polyamides can be made through step-growth polymerization or solid-phase synthesis yielding materials such as nylons, ...
Babar: The Movie
The film features five main songs, performed by Molly Johnson, Judy Tate, The Nylons, and by cast members Stephen Ouimette, ... The songs are (in order of appearance): "Elephantland March" - written by Maribeth Solomon; performed by The Nylons, Judy Tate ... and The Nylons. "Rataxes' Song" - written by Kevan Staples, Marvin Dolgay, and Carole Pope; performed by Charles Kerr. The film ... and The Nylons. "The Best We Both Can Be" - written by Maribeth Solomon; performed by Molly Johnson. "Monkey Business" - ...
Happy Together (song)
The Nylons version rose to No. 79 in the US. The most successful cover was by Captain & Tennille who peaked at No. 53 on the ...
Rotational molding
Other compounds are polyvinyl chloride (PVC) plastisols, nylons, and polypropylene. Order of materials most commonly used by ...
Cellulose
Cotton and synthetics (nylons) each have about 40% market by volume. Other plant fibers (jute, sisal, hemp) represent about 20 ...
Clothing material
It became famous when used in women's stockings ("nylons") in 1940. It was intended to be a synthetic replacement for silk and ...
Nylon riots
Nylons on Sale!" DuPont's announcement indicated that nylons would be available in September and the motto "Nylons by Christmas ... In Louisiana, one household was robbed of 18 pairs of nylons. Similarly, robbery was ruled out as the motive of a murder in ... Women who could not get their hands on nylons resorted to lotions, creams, stick cakes and painting seam lines down their legs ... and Fats Waller's song "When the Nylons Bloom Again" described this situation in poetic terms: Gone are the days when I'd ...
Oakwood Collegiate Institute
Micah Barnes - Singer-songwriter with the bands Loudboy and The Nylons. Alan Bernstein - Cancer researcher. Jully Black - Juno ...
84th Grey Cup
In reality, the halftime act for the contest was The Nylons. "Grey Cup: 1996". Canadian Football League. Archived from the ...
One Fine Day (song)
The Nylons on album 4 On the Floor - Live in Concert (1991). Even in Blackouts on album Zeitgeit's Echo (2005). "One Fine Day" ...
Cool (aesthetic)
... nylons, swing and jazz-the American Cool. To be cool or hip meant "hanging out", pursuing sexual liaisons, displaying the ...
Hold-ups
In popular use, stockings were referred to as "nylons" in the 1940s. In the 1960s, thigh highs retreated towards a more ...
Cramlington
Brentford Nylons also opened a large site in the town in 1968. Boots, the pharmaceuticals manufacturer, opened their plant in ...
nylons Archives - Earth911
Earth911 is a universal resource that helps you find your own shade of green.. We educate and inform consumers, businesses and communities to inspire thought and facilitate earth-positive consumer decisions.. Small changes by thousands of individuals will have a lasting, positive impact. More ideas make less waste!. ...
Browsing by Subject "Nylons"
Layered Nylons:.
nylons Archives » Dollar Store Crafts
Nylons Color Palettes
Candidates, Parody Song Lyrics of Nylons, "Silhouettes"
Nylons Market Size, Share | Industry Forecast 2027
... and opportunities along with detailed analysis of the global nylons market share. ... Nylons can be produced in any form such as fibers or films by the reaction performed with equal parts of amine and carboxylic ... Nylons Market Analysis 2020-2027:. Nylon is a group of synthetic polymers, also known as polyamides. These are thermoplastic ... What are the leading market players active in the nylons market?. *What the current trends will influence the market in the ...
New Nylons for Women | Miiyu | Simons Canada
Nylons 5 (Video 2009) - Release Info - IMDb
Semi Sheer Tights Glossy Nylons | Neon 40 | Wolford
Nylons
NYLONS in Scrabble | Words With Friends score & NYLONS definition
... check NYLONS definition, NYLONS in wwf, Words With Friends score for NYLONS, definition of NYLONS. ... There are 6 letters in NYLONS ( L1N1O1S1Y4 ) To search all scrabble anagrams of NYLONS, to go: NYLONS? ... Scrabble?! NYLONS. Is nylons valid for Scrabble? Words With Friends? Lexulous? WordFeud? Other games?. Scrabble (US/Canada) ... We hope you will find the content on NYLONS in Scrabble , Words With Friends score & NYLONS definition enriching and will use ...
Add a set of black nylons, a few heels, jeans, properties along with a couple of 100 % ralph lauren full zip racing jacket...
Add a set of black nylons, a few heels, jeans, properties along with a couple of 100 % ralph lauren full zip racing jacket ... Add a set of black nylons, a few heels, jeans, properties along with a couple of 100 % ralph lauren full zip racing jacket ... Add a set of black nylons, a few heels, jeans, properties along with a couple of 100 % cotton tops plus youve got the ...
Luxury black nylons | Johny shop
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Pneumatische 8mm Pa Polyamid Luft Schlauch Nylons Chläuche - Buy Nylon Tube,Nylon Hose,Polyamide Tube Product on Alibaba.com
Hit Songs by Nylons - Vancouver Pop Music Signature Sounds
The Nylons appeared in an episode of the TV show, Treasure Island, and sang "The Lion Sleeps Tonight". The next year they ... The Nylons are an a cappella group that formed in 1978, based in Toronto. The original members were all gay men: Dennis Simpson ... The Nylons are an a cappella group that formed in 1978, based in Toronto. The original members were all gay men: Dennis Simpson ... 830: Kiss Him Goodbye by The Nylons Peak Month: July 1987. 10 weeks on CKLGs Vancouver Charts. Peak Position ~ #12. Peak ...
Newest BoobsBetweenArms Vids, Pics and GIFs - We Love Nylons
Wet Nylons and Wet Socks Forum - Muddy High Heels
Nylons
Love Nylons
Welcome to LoveNylons Sexy ladies have that cock thrilling appearance that you simply can t but enjoy. Don t deny yourself the pleasure of devastatingly hot females in slutty pantyhose and stockings. Hundreds of HQ pics will inevitably harden the joy in your pants as you watch the spectacular shaped legs ... ...
Liquid Nylons
Posts about Liquid Nylons written by Noreen Young ... Tag Archives: Liquid Nylons. Post navigation. Want to look like ... Liquid Nylons is a shimmery skin treatment lotion that is more like a magic potion.Used on the legs in the bedroom or when you ... Dont like your legs but love the no hose fashion? TRY Liquid Nylons OR jojoba…your legs will look amazing.. Keep Hydrated and ... Liquid Nylons, MAKEUP, moisturizer, pineapple, pores, pretty, skin, sleep, water Post navigation. ...
Michelle Nylons
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Nylons Forum
... First name: Laouna, Age: 39 yo, City: Vista (CA) We would like to arrange a meeting with you in the woods. Were ... Nylons Forum. First name: Zofja, Age: 21 yo, City: Richmond (CA) Hi there, Im a very beautiful and sexy 21-year-old woman. My ... Nylons Forum. First name: Cyanne, Age: 50 yo, City: Caguas (PR) Welcome men, I am a nymphomaniac girl who is 50 years old and ... Nylons Forum. First name: Sheryane, Age: 26 yo, City: Murrieta (CA) We want a respectful guy or couple for this hot plan, ...
StockingsSexyCappellaNylonTightsTitsPussyLaceHoseBlackMakeBrandWearLingerieMadeWomanShowLeftOriginalDemandPantyhoseTightsSkipLacy NylonsFetishSheerNude in nylonsKnee high nylonsSocksPair of nylonsGirls in nylonsWhite nylonsHuge titsDenierDessousGartersSecretaryBlackUnderwearPolypropyleneHighPairsBeigeSensualLeatherLegsFabric2018TransformLuxuriousModelsWomen
Stockings6
- Welcome to www.layered-nylons.com - the site dedicated to the sexiest UK and European glamour models teasing you wearing both stockings and pantyhose worn together. (tease-pics.com)
- It can be stockings and suspenders worn over pantyhose, or pantyhose worn over holdup nylons. (tease-pics.com)
- Wet nylons, pantyhose, stockings and socks. (muddyhighheels.com)
- Nylons 4k features Ashley Sinclair and her friends who wearing sexy stockings and pantyhose on exclusive 4K fetish videos! (hugescock.com)
- Sock Fixation - Socks, Nylons, Stockings More! (pantybitches.com)
- Afterwards thin stockings called nylons were in great demand by women, as well as for other garments, easy to wash and dry, with no need for ironing. (chemengevolution.org)
Sexy2
- Sexy passionate nylons are an excellent addition to your wardrobe if you want to highlight the beauty of your legs and, of course, also attract your partner with something original, thanks to which you are a little more special for him. (johny-shop.sk)
- Very Sexy Nylons Hot Young. (tubegaylove.com)
Cappella2
- The Nylons are an a cappella group that formed in 1978, based in Toronto. (vancouversignaturesounds.com)
- The Nylons is the debut album from the Canadian a cappella quartet The Nylons released on Attic Records . (disquairegeneralnostalgie.ca)
Nylon3
- However, fluctuating prices of nylon polymers and availability of substitutes with better qualities such as bio-absorbable polymers and polyvinylidenefluoride have gained acceptance in the market act as a threat to the global nylons market. (alliedmarketresearch.com)
- Home Nylon Stylish nylons! (footfetished.com)
- Our woven straps are made using premium nylons and utilise a base and alternate interweaving nylon thread that provides extreme comfort. (stirlingtimepieces.com)
Tights1
- Old nylons or tights make great substitutes for resistance bands. (medlineplus.gov)
Tits1
- White Lycra nylons and big tits! (kittynylons.com)
Pussy1
- Women in nylons sucking dick, nude escorts trf Mount Clemens, mature single males and no joining local women opens pussy girl. (bihadarecipe.net)
Lace1
- Garter lace nylons with a pattern. (johny-shop.sk)
Hose1
- Liquid Nylons is a shimmery skin treatment lotion that is more like a magic potion.Used on the legs in the bedroom or when you don't feel like wearing hose. (wordpress.com)
Black1
- Add a set of black nylons, a few heels, jeans, properties along with a couple of 100 % ralph lauren full zip racing jacket cotton tops plus you've got the essential elements needed to weather any fashion tornado. (mixonline.com)
Make1
- If you see that the nylons inside the package do not have holes, make the holes yourself. (iranpiarom.com)
Brand2
- The brand is also known for reviving the industry's use of nylons. (bagborroworsteal.com)
- My son was naked wearing my brand new expensive Nylons! (jackoffwithmommy.com)
Wear3
- You will want to wear a pair of your mother's nylons. (latterdaysaintmag.com)
- Plus, he shouldn't wear only nylons and no panties or bras! (jackoffwithmommy.com)
- I didn't want to wear nylons, because I would die from the heat outside. (balancevzivote.cz)
Lingerie1
- We are pleased that Vicky's nylons has established itself as one of the few sources of supply when it comes to high quality nylons, garters and lingerie. (vickysnylons.de)
Made1
- The print proposal is rich in sophisticated basics, with almost iridescent organzas made textural by a metallic thread, crepe-paper effect nylons, and sophisticated cloqué ajoure silks. (clericitessuto.it)
Woman1
- Horny woman wearing nylons. (uawlocal145.com)
Show1
- Kitty loves to show her shiny Lycra nylons. (kittynylons.com)
Left1
- The original bass vocalist for The Nylons, he left the band to appear in the Broadway musical Indigo before they became commercially successful. (simonogden.com)
Original1
- By the time the Nylons released their first album, Arnold Robinson was the newcomer joining the other original group members. (vancouversignaturesounds.com)
Demand1
- The economic slowdown caused by the pandemic has led to decline in demand for automobiles, which has led to decline in the use of nylons. (alliedmarketresearch.com)
Pantyhose11
- Welcome to www.layered-nylons.com - the site dedicated to the sexiest UK and European glamour models teasing you wearing both stockings and pantyhose worn together. (tease-pics.com)
- It can be stockings and suspenders worn over pantyhose, or pantyhose worn over holdup nylons. (tease-pics.com)
- Nylons World features fine and elegant European ladies wearing expensive nylons and pantyhose. (discountmags.com)
- Blonde women jeans nylons pantyhose #498170 shows raunchy lust and dreams. (gleb.website)
- Slinky cheeks in pantyhose coed cherry and blonde women jeans nylons pantyhose 36164 belongs to panties xxx images in Full HD format. (gleb.website)
- Here you can find erotic pics for every taste similar to the blonde women jeans nylons pantyhose & save as ophelia pantyhose below. (gleb.website)
- Wet nylons, pantyhose, stockings and socks. (muddyhighheels.com)
- LilyWOW: The Hottest Leggy MILF worldwide in Stockings Nylons and Pantyhose! (anylons.com)
- ASTRID FISHNET SHEERS Luxury Black Pantyhose Micro-net Nylons #ad - Finally stockings that last - our 3d-knitted eco-care recycled yarn pantyhose may cost a little more but saves money because it lasts longer than other brands and creates less disposable waste. (smaerstart900.com)
- Costumes with either nylons, pantyhose or socks with 4 new and exclusive updates each week. (superstockings.com)
- Sexy secretaries in nylons and pantyhose with a new exclusive photo set or video every day. (superstockings.com)
Tights5
- They weren't as thick as the tights/nylons would be, but work perfectly and I have a TON of old t-shirts with lots of fun colors! (howdoesshe.com)
- Pretty Polly Nylons - 10 denier glossy tights, Created today to evoke the vintage glamour of. (tights-store-online.com)
- Beautiful sheer glossy tights developed to give the look of traditional nylons with the fit and feel demanded by women today. (tights-store-online.com)
- Classic elegant pattern - step out and get noticed in trendy micro-net patterned tights with a broad non-sliding waistband, your perfect go-to for everyday patterned nylons to enhance any outfit. (smaerstart900.com)
- Old nylons or tights make great substitutes for resistance bands. (medlineplus.gov)
Skip1
- In a first for Nylons, I am actually going to skip the Good section here. (prowrestlingstudies.org)
Lacy Nylons2
- These pictures provided by Lacy Nylons ! (moms-lingerie.com)
- Sign up Lacy Nylons Now! (moms-lingerie.com)
Fetish2
- This site is not officially affiliated with, associated with, or endorsed by Nylons World Adult Fetish Photo or the publisher. (discountmags.com)
- Glamour and Fetish girls live chat explicit nylons lingerie heels fun! (nhlpcentral.tv)
Sheer3
- Dorothy just rocks in her delicate and sensual stockings and there ain't too many women in seductive sheer nylons that mirror can hold. (girlslovemature.com)
- She is not scared of some fat wet rod and this hawt long legged is just what you want in sexy svelt sheer nylons sex. (girlslovemature.com)
- Luxurious Support Nylons, Full Support Sheer. (jtights.com)
Nude in nylons1
- If you like mature ladies getting nude in nylons, you have come to the right magazine. (discountmags.com)
Knee high nylons1
- Found knee high nylons at Walgreens that come in the candy machine plastic balls. (howdoesshe.com)
Socks1
- Unlike RHT socks, the seam nylons are made on very old flat knitting machines and then sewn. (stocking-society-shop.de)
Pair of nylons2
- You know you have a pair of nylons rolled up in your closet that have a run in them or a hole in the toe, or maybe they're just so brightly colored that you're scared to wear them. (howdoesshe.com)
- If you're facing a time crunch and happen to have a pair of nylons on hand, use them. (thestar.com)
Girls in nylons2
- Naughty girls in nylons on Desktop, Smartphone and Tablet. (nylonup.com)
- Horny girls in nylons are waiting for you. (nylonup.com)
White nylons1
- Possibly some white nylons and shoes but again, not really necessary. (petit-animal.com)
Huge tits1
- Miss Hybrid Sybian ride, huge tits and sexy nylons. (misshybrid.net)
Denier1
- Nylons are classified in the denier system and come in a wide range of weights with 200 denier being our lightest and our heaviest being our ballistic nylon which has many industrial uses. (fabrictime.com)
Dessous1
- Wir freuen uns, dass Vicky´s Nylons sich als eine der wenigen Bezugsquellen etabliert hat, wenn es um hochwertige Nylons, Strumpfhaltern und Dessous geht. (vickysnylons.de)
Garters1
- We are pleased that Vicky's nylons has established itself as one of the few sources of supply when it comes to high quality nylons, garters and lingerie. (vickysnylons.de)
Secretary3
- Last night, my Secretary prepared for her work day by washing her black fully-fashioned Secrets In Lace nylons and air-drying them, along with her Kiss Me Deadly garter belt. (substack.com)
- I gave her a thorough performance review this morning, and those nylons (and my Secretary) are now well-used. (substack.com)
- Hot secretary Jenny reveals her gorgeous black nylons and sexy high heel shoes. (dressed-ladies.com)
Black4
- Most Recent Black Nylons Films Videos! (deadsexyclips.com)
- Elegant legs in black nylons. (deadsexyclips.com)
- Black Nylons. (deadsexyclips.com)
- The 28-year-old singer sported a furry white coat with matching bottoms and a pair of black fishnet nylons while heading into the concert venue located off the famed Sunset Boulevard. (thegirlsun.com)
Underwear1
- One woman even packed her nylons and underwear in plastic bags. (uexpress.com)
Polypropylene1
- A fantastic range of Nylons, Solution Dyed Nylons, Wools and Polypropylene Carpets. (accentcarpets.net.au)
High6
- Increasing demand for Nylons in high performance segment coupled with high investment in R&D of Nylons is fuelling the market growth. (makenix.com)
- The market is rising rapidly in the global market due to the increase in high demand for Nylons in various highly productive applications. (makenix.com)
- You can't be wearing high-heel shoes with your toes in nylons," he added. (ktar.com)
- Extensive collection of Latex/Leather, High Heels & Boots, Nylons. (kitteens.org)
- frauen männer, pasewalk Dildo selber machen sex mannheim Layaspot 2, Tantramassage in Sie in berlin pornos spanking geschichten nylons in high heels kostenlos. (heritagebangladesh.co)
- I cater for all activities in foot worship, whether it be trampling with or without shoes, foot massage, toe sucking, face smothering, sniffing the aroma after a shopping trip or gym session, seeing them in nylons and high heels and so much more. (londonmistresszone.co.uk)
Pairs1
- They have great sustain and volume but they may drown out nylons because of this :P Pairs really nice with fluorocarbon strings. (tapestrymusic.com)
Beige1
- With my hot beige nylons, I tell you in this Jerk Off Instruction video how to fuck me anal! (anny-aurora.com)
Sensual1
- Fully Fashioned Nylon Stockings - The sensual classics among the real nylons! (stocking-society-shop.de)
Leather2
Legs1
- Do you like beautiful slim legs in sexy nylons? (anylons.com)
Fabric1
- Taking a pair of old nylons, I rubbed the nylon fabric over top of the deodorant mark for about 20 seconds. (thestar.com)
20181
- Nylons had a market share of 8.2% in 2018 and is expected to grow with a CAGR of 9.6% throughout the forecast period. (makenix.com)
Transform2
- A Nigerian identified as Emeka Nelson took to Facebook to share photos of a machine he invented that can transform plastics,nylons and more into kerosene,petrol and more.He wrote. (nairaland.com)
- Emily is back to show us how to transform your old nylons into a new head accessory! (howdoesshe.com)
Luxurious2
- Nylons without seam - luxurious ladies stockings for every day! (stocking-society-shop.de)
- Long before the nylon stocking was invented, silk stockings were the luxurious predecessors of the nylons. (stocking-society-shop.de)
Models2
- Hot models in nylons updated daily for your enjoyment. (superstockings.com)
- Then Only Opaques is just for you, with hot models in nylons updated daily for your enjoyment. (pantyhosehouse.com)
Women2
- Women in nylons make me cum. (samui-select.com)
- Women in nylons wanting sex. (billiger-einkaufen.info)