Suture Techniques
Caprolactam
Polydioxanone
Aminocaproates
Enzymes, Immobilized
Flavobacterium
Aminocaproic Acid
Filtration
DNA Probes
Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.
Polyglactin 910
Surgical Staplers
Surgical Wound Dehiscence
Nucleic Acid Hybridization
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
T-Lymphocytes
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Tissue Adhesives
Cyanoacrylates
Nylons
Abdominal Wall
The outer margins of the ABDOMEN, extending from the osteocartilaginous thoracic cage to the PELVIS. Though its major part is muscular, the abdominal wall consists of at least seven layers: the SKIN, subcutaneous fat, deep FASCIA; ABDOMINAL MUSCLES, transversalis fascia, extraperitoneal fat, and the parietal PERITONEUM.
Plastics
Polymeric materials (usually organic) of large molecular weight which can be shaped by flow. Plastic usually refers to the final product with fillers, plasticizers, pigments, and stabilizers included (versus the resin, the homogeneous polymeric starting material). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Polyethylene Terephthalates
Concanavalin A
Rosette Formation
The in vitro formation of clusters consisting of a cell (usually a lymphocyte) surrounded by antigenic cells or antigen-bearing particles (usually erythrocytes, which may or may not be coated with antibody or antibody and complement). The rosette-forming cell may be an antibody-forming cell, a memory cell, a T-cell, a cell bearing surface cytophilic antibodies, or a monocyte possessing Fc receptors. Rosette formation can be used to identify specific populations of these cells.
Lymphocytes
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Glutaral
Specimen Handling
Rumen
The first stomach of ruminants. It lies on the left side of the body, occupying the whole of the left side of the abdomen and even stretching across the median plane of the body to the right side. It is capacious, divided into an upper and a lower sac, each of which has a blind sac at its posterior extremity. The rumen is lined by mucous membrane containing no digestive glands, but mucus-secreting glands are present in large numbers. Coarse, partially chewed food is stored and churned in the rumen until the animal finds circumstances convenient for rumination. When this occurs, little balls of food are regurgitated through the esophagus into the mouth, and are subjected to a second more thorough mastication, swallowed, and passed on into other parts of the compound stomach. (From Black's Veterinary Dictionary, 17th ed)
Polyethylene
A vinyl polymer made from ethylene. It can be branched or linear. Branched or low-density polyethylene is tough and pliable but not to the same degree as linear polyethylene. Linear or high-density polyethylene has a greater hardness and tensile strength. Polyethylene is used in a variety of products, including implants and prostheses.
Oligonucleotide Array Sequence Analysis
Plasmapheresis
Glucose Oxidase
An enzyme of the oxidoreductase class that catalyzes the conversion of beta-D-glucose and oxygen to D-glucono-1,5-lactone and peroxide. It is a flavoprotein, highly specific for beta-D-glucose. The enzyme is produced by Penicillium notatum and other fungi and has antibacterial activity in the presence of glucose and oxygen. It is used to estimate glucose concentration in blood or urine samples through the formation of colored dyes by the hydrogen peroxide produced in the reaction. (From Enzyme Nomenclature, 1992) EC 1.1.3.4.
B-Lymphocytes
Lymphocyte Activation
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
Membranes, Artificial
Oligonucleotide Probes
Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.
Tensile Strength
Poaceae
Astigmatism
Unequal curvature of the refractive surfaces of the eye. Thus a point source of light cannot be brought to a point focus on the retina but is spread over a more or less diffuse area. This results from the radius of curvature in one plane being longer or shorter than the radius at right angles to it. (Dorland, 27th ed)
Cytotoxicity, Immunologic
Granulocytes
Biodegradation, Environmental
Cytotoxicity Tests, Immunologic
Leukocytes
Neutrophils
Gene Expression Profiling
Cornea
The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)
Antilymphocyte Serum
Cattle
Chemotactic Factors
Immunosuppression
Deliberate prevention or diminution of the host's immune response. It may be nonspecific as in the administration of immunosuppressive agents (drugs or radiation) or by lymphocyte depletion or may be specific as in desensitization or the simultaneous administration of antigen and immunosuppressive drugs.
Lymphocyte Culture Test, Mixed
Measure of histocompatibility at the HL-A locus. Peripheral blood lymphocytes from two individuals are mixed together in tissue culture for several days. Lymphocytes from incompatible individuals will stimulate each other to proliferate significantly (measured by tritiated thymidine uptake) whereas those from compatible individuals will not. In the one-way MLC test, the lymphocytes from one of the individuals are inactivated (usually by treatment with MITOMYCIN or radiation) thereby allowing only the untreated remaining population of cells to proliferate in response to foreign histocompatibility antigens.
Dose-Response Relationship, Immunologic
Anopheles gambiae
Antigens, Thy-1
Immunization, Passive
Microscopy, Electron, Scanning
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
Lectins
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
Killer Cells, Natural
Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.
Polymers
Mice, Inbred Strains
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
Mitogens
Isoantigens
Cells, Cultured
Mosquito Control
Macrophages
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Immunity, Cellular
Gene Library
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Base Sequence
Hypersensitivity, Delayed
Dietary Proteins
Luminescent Measurements
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Arterial Occlusive Diseases
Catheterization
Leukocyte Count
Materials Testing
Sensitivity and Specificity
Antigens, Surface
T-Lymphocytes, Regulatory
Surface Properties
Chemotaxis, Leukocyte
Serum Albumin, Bovine
DNA
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Rabbits
Random Allocation
Phagocytosis
Indomethacin
Thymus Gland
A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.
Sheep
Rats, Wistar
Antibody Formation
Disease Models, Animal
Reproducibility of Results
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
Plasmids
Lymph Nodes
Rats, Inbred Strains
Temperature
Culture Media
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Blotting, Southern
Monocytes
Immune Tolerance
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
Swine
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Polymorphism, Restriction Fragment Length
DNA, Complementary
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Postoperative Complications
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Species Specificity
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Immunoglobulin G
Gene Expression
Brain
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
Interleukin-10 and antigen-presenting cells actively suppress Th1 cells in BALB/c mice infected with the filarial parasite Brugia pahangi. (1/451)
Infection with the third-stage larvae (L3) of the filarial nematode Brugia results in a Th2-biased immune response in mice and humans. Previously we have shown that the production of interleukin 4 (IL-4) is critical for down-regulating polyclonal Th1 responses in L3-infected mice. However, the in vitro neutralization of IL-4 did not fully recover the defective polyclonal Th1 responses, nor did it result in the production of any antigen (Ag)-specific Th1 cytokines, suggesting that perhaps infection with L3 does not result in priming of Th1 cells in vivo. In this study, we analyzed the role of IL-10 and Ag-presenting cells (APCs) in the spleen as additional factors controlling the Th2 bias in infected mice. Our data show that IL-10 and APCs also contribute to the suppression of mitogen-driven Th1 responses of spleen cells from infected mice. In addition, the neutralization of IL-10 or the replacement of the resident APC population from spleen cell cultures resulted in the production of Ag-specific Th1 cytokines. Irradiated spleen cells from either L3-infected or uninfected mice were able to restore Ag-specific Th1 responses in vitro. Therefore, it appears that Brugia-reactive Th1 cells are primed following infection with L3, but are actively suppressed in vivo by a mechanism that involves IL-10 and the resident APC population, but not IL-4. These results indicate that a complex interplay of cytokines and cell populations underscores the Th2-polarized response in L3-infected mice. (+info)Inhibition of Ets-1 DNA binding and ternary complex formation between Ets-1, NF-kappaB, and DNA by a designed DNA-binding ligand. (2/451)
Sequence-specific pyrrole-imidazole polyamides can be designed to interfere with transcription factor binding and to regulate gene expression, both in vitro and in living cells. Polyamides bound adjacent to the recognition sites for TBP, Ets-1, and LEF-1 in the human immunodeficiency virus, type 1 (HIV-1), long terminal repeat inhibited transcription in cell-free assays and viral replication in human peripheral blood lymphocytes. The DNA binding activity of the transcription factor Ets-1 is specifically inhibited by a polyamide bound in the minor groove. Ets-1 is a member of the winged-helix-turn-helix family of transcription factors and binds DNA through a recognition helix bound in the major groove with additional phosphate contacts on either side of this major groove interaction. The inhibitory polyamide possibly interferes with phosphate contacts made by Ets-1, by occupying the adjacent minor groove. Full-length Ets-1 binds the HIV-1 enhancer through cooperative interactions with the p50 subunit of NF-kappaB, and the Ets-inhibitory polyamide also blocks formation of ternary Ets-1. NF-kappaB.DNA complexes on the HIV-1 enhancer. A polyamide bound adjacent to the recognition site for NF-kappaB also inhibits NF-kappaB binding and ternary complex formation. These results broaden the application range of minor groove-binding polyamides and demonstrate that these DNA ligands are powerful inhibitors of DNA-binding proteins that predominantly use major groove contacts and of cooperative protein-DNA ternary complexes. (+info)Sensitivity issues in DNA array-based expression measurements and performance of nylon microarrays for small samples. (3/451)
DNA or oligonucleotide arrays are widely used for large-scale expression measurements, using various implementations: macroarrays in which DNA is spotted onto nylon membranes of relatively large dimensions (with radioactive detection) on the one hand; microarrays on glass slides and oligonucleotide chips, both used with fluorescent probes, on the other hand. Nylon micro-arrays with colourimetric detection have also been described recently. The small physical dimensions of miniaturized systems allow small hybridization volumes (2-100 microl) and provide high probe concentrations, in contrast to macroarrays. We show, however, that actual sensitivity (defined as the amount of sample necessary for detection of a given mRNA species) is in fact similar for all these systems and that this is mostly due to the very different amounts of target material present on the respective arrays. We then demonstrate that the combination of nylon microarrays with(33)P-labelled radioactive probes provides 100-fold better sensitivity, making it possible to perform expression profiling experiments using submicrogram amounts of unamplified total RNA from small biological samples. This has important implications in basic and clinical research and makes this alternative approach particularly suitable for groups operating in an academic context. (+info)Functional and morphologic characteristics of the leukemic cells of a patient with acute monocytic leukemia: correlation with clinical features. (4/451)
The clinical course of a patient with acute monocytic leukemia and prominent infiltration of the skin and testes is described. In vitro studies demonstrated that the circulating monocyte precursors were capable of adherence to nylon fibers, and phagocytosis of bacteria and latex particles. In vivo, migration of leukemic cells to skin windows was observed. Extreme nuclear folding, marked surface activity, and morphologic features suggesting nuclear and cytoplasmic maturation were seen by light and electron microscopy. The presence of morphologically and functionally more differentiated monocytic cells may account for the marked tiuuse invasion in this patient and, possibly, in other patients with monocytic leukemia. (+info)In vitro cytotoxicity of textile paint components linked to the "Ardystil syndrome". (5/451)
The spraying of a paint formula (Acramin F system) had led to severe pulmonary disease in textile printing sprayers in Spain and Algeria (Ardystil syndrome). In order to elucidate the underlying mechanisms of the toxicity of this paint and its main polymeric components, Acramin FWR, Acramin FWN, Acrafix FHN, and Acramoll W, we have undertaken studies using a battery of different cell-types and assessing in vitro cytotoxicity by measuring LDH leakage. This study shows that, as in in vivo studies, the three polycationic paint components, Acramin FWR (a polyurea), Acramin FWN (a polyamide-amine), and Acrafix FHN (a polyamine) exhibited considerable cytotoxicity (LC50 generally below 100 microg/ml for an incubation of 20-24 h) in vitro, while Acramoll W, which is not a polycation, was almost non-toxic (in the concentration range tested). The cytotoxicity was comparable in primary cultures of rat and human type II pneumocytes and alveolar macrophages as well as in the pulmonary cell line A549 and the hepatic cell line HepG2. In human erythrocytes, the toxicity was less pronounced. We speculate that the multiple positive charges play an important role in the toxic mechanism. It is concluded that Acramin FWR and Acramin FWN have similar intrinsic toxicity and that these polymeric compounds, which have no irritant properties or systemic toxicity when given orally, exert a high, unexpected, degree of cytotoxicity. (+info)Local anaesthetic effect of topical amethocaine gel in neonates: randomised controlled trial. (6/451)
AIM: To assess the efficacy of amethocaine as a topical local anaesthetic in neonates. METHODS: A randomised, double blind controlled trial compared 4% amethocaine gel (Ametop) with placebo in 60 healthy neonates (29 to 42 weeks of gestation) in the first week after birth. Either 1.5 g 4% w/w amethocaine (gel) or 1.5 g placebo gel were applied to the dorsum of one foot. No gel was applied to the other foot. Each foot was occluded and left for one hour. Local anaesthesia was then assessed by eliciting the cutaneous withdrawal reflex in response to stimulation with a series of graded nylon filaments (von Frey hairs). The reflex was first elicited from the control and then the treated foot. The difference in filament thickness and deforming weight required to elicit the reflex was recorded. RESULTS: In infants treated with amethocaine, 17 of 31 (54. 8%) showed evidence of local anaesthetic action compared with five of 29 (17.2%) in the placebo group (p=0.003). The mean difference in deforming weight required to elicit the reflex was 18.8 g in the amethocaine group compared with 3.9 g in the placebo group (p=0.02). The apparent local anaesthetic action of the placebo can be explained by habituation to repeated stimulation. CONCLUSIONS: It is concluded that topical amethocaine gel has a local anaesthetic action on neonatal skin which merits further investigation. An effective and safe surface local anaesthetic would be valuable for the relief of procedure related pain in neonates. (+info)In-source decay of hyperbranched polyesteramides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. (7/451)
Hyperbranched polyesteramides (DA2), prepared from hexahydrophthalic anhydride (D) and diisopropanolamine (A) have been characterized, by use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), field desorption (FD)-MS, and electrospray ionization (ESI)-MS. MALDI of polyesteramides produces protonated molecules. The spectra show a complex chemical composition distribution and end-group distribution which are mainly composed of two series of homologous oligomers DnA(n)+1 - mH2O and DnA(n) - mH2O, where m = 1-2. Signals from protonated molecules DnAn+1 and DnAn are almost absent in the MALDI spectrum, whereas these ions are responsible for the base peak of DnA(n)+1 - mH2O and DnA(n) - mH2O (m = 1-2) clusters in the ESI spectrum. The absence of -OH end-groups signals in the MALDI spectrum is due to a metastable decay of protonated DnA(n)+1 and DnAn ions in the ion source of the MALDI mass spectrometer prior to ion extraction. In-source decay results in the formation of protonated lower DnA(n)+1 - mH2O and DnA(n) - mH2O oligomers and their corresponding neutrals, leading to wrong conclusions concerning the relative end-group distribution as a function of the degree of polymerization and the chemical composition. (+info)Sequence specific alkylation of DNA by hairpin pyrrole-imidazole polyamide conjugates. (8/451)
BACKGROUND: Pyrrole-imidazole polyamides are synthetic ligands that recognize predetermined sequences in the minor groove of DNA with affinities and specificities comparable to those of DNA-binding proteins. As a result of their DNA-binding properties, polyamides could deliver reactive moieties for covalent reaction at specific DNA sequences and thereby inhibit DNA-protein interactions. Site-specific alkylation of DNA could be a useful tool for regulating gene expression. As a minimal first step, we set out to design and synthesize a class of hairpin polyamides equipped with DNA alkylating agents and characterize the specificity and yield of covalent modification. RESULTS: Bis(dichloroethylamino)benzene derivatives of the well-characterized chlorambucil (CHL) were attached to the gamma turn of an eight-ring hairpin polyamide targeted to the HIV-1 promoter. We found that a hairpin polyamide-CHL conjugate binds and selectively alkylates predetermined sites in the HIV promoter at subnanomolar concentrations. Cleavage sites were determined on both strands of a restriction fragment containing the HIV-1 promoter, revealing good specificity and a high yield of alkylation. CONCLUSIONS: The ability of polyamide-CHL conjugates to sequence specifically alkylate double-stranded DNA in high yield and at low concentrations sets the stage for testing their use as regulators of gene expression in cell culture and ultimately in complex organisms. (+info)
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119050020 - DURATOOL - Machine Screw, M5, Nylon 6.6 (Polyamide 6.6), 20 mm, Cheese Head Slotted | Farnell element14
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Patent US5061763 - Stain resistant treatment for polyamide fibers - Google Patents
Indian Patents. 215443:A METHOD OF MASS-COLOURING POLYAMIDE MATERIAL AND A POLYAMIDE MATERIAL COLOURED THEREBY
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US Patent for Method for producing polyamides Patent (Patent # 7,470,765 issued December 30, 2008) - Justia Patents Search
BASF Patents Titania-pigmented Polyamide
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Global Bio-Polyamide, Specialty Polyamide & Precursors Sales... - Chemical Industry News
The Nylons - Wikipedia
The Nylons are an a cappella group founded in 1978 in Toronto, Ontario, Canada, best known for their covers of pop songs such ... The Nylons appeared in Season 3 of the show on the "Treasure Island" episode, singing "The Lion Sleeps Tonight". These songs ... The Nylons original lineup consisted of Claude Morrison (tenor), Paul Cooper (baritone; born James Paul Cooper in Pikeville, ... In October 24, 1992, The Nylons sang "O Canada" at Atlanta-Fulton County Stadium in Atlanta, Georgia, preceding Game 6 of the ...
The Nylons (album) - Wikipedia
REMEMBER Lyrics - NYLONS | eLyrics.net
Nylons Remember lyrics & video : It seems like the other day That my baby went away Went away across the sea It seems like a ... Top Nylons LyricsYoure The One I Turn ToBlue RadioBecauseBake PotatoBack On TrackAnother Night Like ThisAmazonAi No CorridaA ... NYLONS. Remember Lyrics. It seems like the other day. That my baby went away. Went away across the sea. It seems like a year ...
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Nylons, light blue/white/pink Smart stockings for riders made of high elastic material that adapts perfectly to the foot. ... Caro socks Nylons. Product no.: UG-11200004-334 Be the first to rate this product ... Caro socks "Nylons", light blue/white/pink Smart stockings for riders made of high elastic material that adapts perfectly to ...
Ur-Turn: Not Your Grandmother's Nylons - The Truth About Cars
Most of the readers here probably know that this is not the first time that plastic coolant fittings have failed in a spectacular fashion. In the early 2000s Ford settled a class action lawsuit brought by owners of various vehicles that had 4.6L V-8s with intake manifolds made of Nylon. These intake manifolds failed at the coolant passage. The intake manifold was replaced with a new design in which the coolant passage was made of… you guessed it, aluminum.. DuPont was at the forefront of the drive to use Nylon, specifically Glass Fiber reinforced Nylon 6/6 for engine parts starting with radiator end caps which were first adopted by Ford in 1986. Later, in 1996, the first intake manifolds manufactured from the same material started appearing on cars. Nylon intake manifolds have since become ubiquitous and I think that the technology is proven at this point. There are several advantages of using plastic in place of steel or aluminum for intake manifolds. The plastic intake manifolds are ...
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Wearing nylons to an interview?
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Handbook of Engineering and Speciality Thermoplastics: Volume 4: Nylons / Edition 1 by Sabu Thomas | 9780470639252 | Hardcover ...
This final volume in the Handbook of Engineering and Speciality Thermoplastics covers Nylons and details the ... This final volume in the Handbook of Engineering and Speciality Thermoplastics covers Nylons and details the developments of ... 1. Engineering and Specialty Thermoplastics: Nylons 1. P. M. Visakh and Sabu Thomas. 1.1 Polyamide-imides 1. 1.2 Polyetherimide ... Handbook of Engineering and Speciality Thermoplastics: Volume 4: Nylons / Edition 1. by Sabu Thomas, Visakh P. M.Sabu Thomas ...
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Synchrotron radiation studies on even-odd and odd-even nylons
Real-time temperature dependence of X-ray diffraction patterns for nylons 65 and 56 was studied by synchrotron radiation to ... CitacióFranco, M. [et al.]. Synchrotron radiation studies on even-odd and odd-even nylons. "IOP Conference Series: Materials ... Significant differences were found between the temperature dependence of both nylons, although a transition towards a ... fiber diffraction patterns of this sheet structure were observed in several polyamides derived from odd units such as nylons 65 ...
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Happy birthday nylons and 5 other female fashion fails | Lifestyle | DW | 15.05.2015
... but do nylons really need to stick around for another 75 years? DWs Kate Müser cleans out her closet. ... Happy birthday nylons and 5 other female fashion fails. They may have revolutionized womens fashion, but do nylons really need ... While Im grateful for the ways nylons may have opened doors for my gender, why did it have to be sweaty, scratchy nylons? ... Keywords nylon stockings, fashion, style, nylons, women, lifestyle * Share Send Facebook Twitter Google+ More Whatsapp Tumblr ...
KAKEN - Research Projects | Development of Biodegradable Nylons (KAKENHI-PROJECT-07555673)
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Lost in the Ozone...: Giuliani's Rudy-in-Nylons Skit Starred Some Witless Crooks by Tom Robbins - Village Voice
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Volume 4 on Nylons is a unique compilation and covers many of the recent technical research accomplishments in the area of ... 1. Engineering and Specialty Thermoplastics: Nylons 1. P. M. Visakh and Sabu Thomas. 1.1 Polyamide-imides 1. 1.2 Polyetherimide ... This final volume in the Handbook of Engineering and Speciality Thermoplastics covers Nylons and details the developments of ... nylons). Recent advances in the development and characterization of multi component polymer blends are discussed in detail. ...
Fully Fashioned Nylons | The Official Louise Jenson
All images on this site are original content or licensed material. We hold model releases and IDs for every model and are compliant to 18 USC U2257. The records required by Section 2257 of Title 18, United States Code, with respect to this site, and all graphical materials associated therewith on which this label appears, are kept by the custodian of records: USC 18 Title 2257 regulations. Billing Support: CCBill , Login / Password Errors: email me. Home , Latest Updates , Support , Members , Privacy , Terms © Copyright theofficiallouisejenson.com, All Rights Reserved. Site developed by Fusion Webmasters ...
Polyamides3
- However, typical fiber diffraction patterns of this sheet structure were observed in several polyamides derived from odd units such as nylons 65 and 56. (upc.edu)
- Real-time temperature dependence of X-ray diffraction patterns for nylons 65 and 56 was studied by synchrotron radiation to gain understanding of the Brill transition usually occurring in polyamides. (upc.edu)
- Synthetic polyamides, nylons, especially those having relatively simple repeating units, are generally hard to be decomposed under natural environmental conditions either through biological or through hydrolytic processes. (nii.ac.jp)
Vintage nylons1
- Vintage Nylons - Free Vintage XXX Images! (zitar-metiz.ru)
Ferroelectric nylons2
- Although nylon polymers have found over the years significant commercial applications in fabrics and fibers, their application in electronic devices was hindered because it was impossible to create high quality thin films of ferroelectric nylons by solution processing. (mpg.de)
- The scientists subjected the prototypes of the capacitors to extended stress cycles and demonstrated robustness of ferroelectric nylons under millions of operation cycles. (mpg.de)
Polymers4
- Volume 4 on Nylons is a unique compilation and covers many of the recent technical research accomplishments in the area of engineering polymers, such as nitrogen containing main chain polymers (Nylons). (barnesandnoble.com)
- This book summarizes many of the recent technical research accomplishments in the area of engineering polymers, such as oxygen containing main chain polymers (nylons). (wiley-vch.de)
- Nylons, a family of synthetic polymers, were first introduced in the 1940s' for women's stockings and are nowadays among the most widely used synthetic fibers in textiles. (mpg.de)
- The most important amide polymers are the nylons, an extremely versatile class of material that is an indispensable fibre and plastic . (britannica.com)
Sheer10
- Wearing a pair of sheer nylons can't hurt anything, but if they are super conservative not wearing them could have them question if you're the right fit for the workplace environment. (weddingbee.com)
- Them you need a pair of sheer champagne seamed nylons to finish your look. (whatkatiedid.com)
- Watch full-lenght movie in HDV quality!Barbara looks like a doll in her electric blue satin gown, but soon this wicked prankster rides it up to show off the tops of her sheer gartered nylons and bare buttocks. (girlslovemature.com)
- Our sheer compression socks blend in with any outfit, just like regular nylons. (spressowear.ca)
- Dead Stock 50's60's Sheer Nylons,50's60's Sheer Nylons Dead Stock,americanvintageclassics,com RWL305,Label: Round The Clock - They Fit Dead Stock Sheer Nylons - Rhapsody Size: 9 - Trim Slim Condition: Unworn -See more Vintage Lingerie at: www,Get Great Savings,Discounted price,Quality products,Get the best choice,Enjoy GREAT PRICES and FREE SHIPPING! (ladolcevitaathens.com)
- Dead Stock 50's/60's Sheer Nylons. (ladolcevitaathens.com)
- Label: Round The Clock - They Fit Dead Stock Sheer Nylons - Rhapsody Size: 9 - Trim Slim Condition: Unworn -See more Vintage Lingerie at: www.americanvintageclassics.com RWL305. (ladolcevitaathens.com)
- EASY TO INSTALL : Since it's solar powered and requires no wiring, This sun visor have a vanity light , ✐ Material: Primary: Gold, Dead Stock 50's60's Sheer Nylons . (ladolcevitaathens.com)
- To ensure authenticity of items being purchased, Dead Stock 50's60's Sheer Nylons . (ladolcevitaathens.com)
- I think my sheer white panties are so cute and I know how much you like panties under my nylons. (sadiespanties.com)
Wear nylons5
- poll: Should I wear nylons to a big interview? (weddingbee.com)
- However, should I wear nylons? (weddingbee.com)
- where I am from is pretty conservative and I dont think I have ever seen a woman wear nylons. (weddingbee.com)
- I'd definitely wear nylons since I know a few people who work for hospitals and nylons are hospital policy. (weddingbee.com)
- OK I'm going to wear nylons then! (weddingbee.com)
Heels1
- Having my wife dress in great nylons and high heels. (experienceproject.com)
20161
- In fall and winter 2016, The Nylons toured and performed their farewell show throughout Canada. (wikipedia.org)
Sexy2
- 2,5 kg Mega Sexy Nylons Rock'n'Roll Latex Fotografie vom Goliath. (burlesque-fashion.de)
- Watch full-lenght movie in HDV quality!When it comes to sexy soft stockings no floozy in nylons looks better inthem than Stella here and that should make you pop a boner right away. (girlslovemature.com)
PLASTICS2
- It you have a seepage pit for your septic system, you have to make sure that plastics and nylons do not make it in there or you will have issues to deal with that may be require costly replacements or repairs. (septictankcare.net)
- As plastics the nylons still find employment as an engineering plastic-for example, in bearings, pulleys, gears, zippers, and automobile fan blades. (britannica.com)
Cappella1
- The Nylons is the debut album from the Canadian a cappella quartet The Nylons released on Attic Records. (wikipedia.org)
Nylon3
- Molybdenum disulphide-filled and oil-filled nylons have enhanced wear properties, while heat stabilized nylon will withstand higher operating temperatures. (skjdindia.com)
- Nylons unterm rock pornokino in berlin, beliebte Suchen Sm Sex Sex Gratis Nylon. (buffaloqtl.org)
- Nylon was first used commercially in a nylon- bristled toothbrush in 1938, [11] [12] followed more famously in women's stockings or "nylons" which were shown at the 1939 New York World's Fair and first sold commercially in 1940. (wikipedia.org)
Stockings1
- Caro socks "Nylons", light blue/white/pink Smart stockings for riders made of high elastic material that adapts perfectly to the foot. (strato.de)
Women's1
- They may have revolutionized women's fashion, but do nylons really need to stick around for another 75 years? (dw.com)
Nitrogen1
- For example, polyesters have oxygen atoms and nylons have nitrogen atoms in the backbone in addition to carbon atoms (Figure 4). (encyclopedia.com)
Handbook of Engineering1
- This final volume in the Handbook of Engineering and Speciality Thermoplastics covers Nylons and details the developments of the last decade with respect to their polymerization, properties, synthesis, and applications. (barnesandnoble.com)
Exhibit1
- Products using Shakespeare® Nylons exhibit excellent dimensional stability and thermal properties, suitable for either large or micro precision parts. (shakespearenylons.com)
Fatigue resistance1
- Metalon MC is better impact strength and fatigue resistance compared with other cast nylons in the range. (metalonplastics.com)
Panties1
- well do what you love to do when you see a pair of panties under nylons. (sadiespanties.com)
Properties1
- Our injection molding grade nylons are suitable for large and micro precision parts, and are designed to offer exceptional dimensional stability, optimal viscosity and prime thermal properties. (shakespearenylons.com)
Good1
- Watch her spread-eagling in her good nylons while drilling her insane little pooper! (girlslovemature.com)
Pair1
- There were less conservative firms but if you are right and this is a conservative area then I wouldn't risk it for the cost of a pair of nylons. (weddingbee.com)
Models1
- The way this hottie models these silky stocking nylons is enough to make you want to jump in there and start wild hardcore fucking her until you break a sweat. (girlslovemature.com)
Female1
- Having spent my early life in single sex boarding schools (I'm male), I had always fantasised about the tactile mystery of women in nylons, as well as the female anatomy in general. (experienceproject.com)
Girls3
- The hottest girls getting off in their nylons in front of your eyes. (nylonup.com)
- Naughty girls in nylons on Desktop, Smartphone and Tablet. (nylonup.com)
- Horny girls in nylons are waiting for you. (nylonup.com)
Dress1
- I've worked in Bay street (Canada's version of Wall Street) law firms and part of our dress code always included nylons with skirts. (weddingbee.com)
Clean2
- She makes him worship her tan nylons, lick and suck them clean. (bratperversions.com)
- Use those nylons to clean around the house . (familyhandyman.com)
High1
- Significant differences were found between the temperature dependence of both nylons, although a transition towards a monoclinic structure characterized by a single equatorial reflection around 0.425-0.420 nm was observed to occur at high temperatures. (upc.edu)
Happy1
- The Nylons (1982) One Size Fits All (1982) - #59 AUS Seamless (1984) - #133 Billboard 200 Happy Together (1987) - #43 Billboard 200 Rockapella (1989) 4 on the Floor (1991, live) Live to Love (1992) The Best of the Nylons (1993) Illustrious (1993) Another Fine Mesh (1994) Harmony: The Christmas Songs (1994) Because. (wikipedia.org)
Canada1
- In October 24, 1992, The Nylons sang "O Canada" at Atlanta-Fulton County Stadium in Atlanta, Georgia, preceding Game 6 of the 1992 World Series, in which their hometown team, the Toronto Blue Jays, won their first championship. (wikipedia.org)
Show1
- On May 30, 2014, the Nylons performed a show in Toronto which included the participation of every living past or present member of the band. (wikipedia.org)