Viral proteins found in either the NUCLEOCAPSID or the viral core (VIRAL CORE PROTEINS).
A protein-nucleic acid complex which forms part or all of a virion. It consists of a CAPSID plus enclosed nucleic acid. Depending on the virus, the nucleocapsid may correspond to a naked core or be surrounded by a membranous envelope.
A species of CORONAVIRUS causing atypical respiratory disease (SEVERE ACUTE RESPIRATORY SYNDROME) in humans. The organism is believed to have first emerged in Guangdong Province, China, in 2002. The natural host is the Chinese horseshoe bat, RHINOLOPHUS sinicus.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Peptides whose amino and carboxy ends are linked together with a peptide bond forming a circular chain. Some of them are ANTI-INFECTIVE AGENTS. Some of them are biosynthesized non-ribosomally (PEPTIDE BIOSYNTHESIS, NON-RIBOSOMAL).
A viral disorder characterized by high FEVER, dry COUGH, shortness of breath (DYSPNEA) or breathing difficulties, and atypical PNEUMONIA. A virus in the genus CORONAVIRUS is the suspected agent.
Proteins found mainly in icosahedral DNA and RNA viruses. They consist of proteins directly associated with the nucleic acid inside the NUCLEOCAPSID.
The type species of the genus HANTAVIRUS infecting the rodent Apodemus agrarius and humans who come in contact with it. It causes syndromes of hemorrhagic fever associated with vascular and especially renal pathology.
The outer protein protective shell of a virus, which protects the viral nucleic acid.
An acute viral infection in humans involving the respiratory tract. It is marked by inflammation of the NASAL MUCOSA; the PHARYNX; and conjunctiva, and by headache and severe, often generalized, myalgia.
The type species of the genus INFLUENZAVIRUS A that causes influenza and other diseases in humans and animals. Antigenic variation occurs frequently between strains, allowing classification into subtypes and variants. Transmission is usually by aerosol (human and most non-aquatic hosts) or waterborne (ducks). Infected birds shed the virus in their saliva, nasal secretions, and feces.
The type species of LENTIVIRUS and the etiologic agent of AIDS. It is characterized by its cytopathic effect and affinity for the T4-lymphocyte.
Ribonucleic acid that makes up the genetic material of viruses.
Proteins encoded by the GAG GENE of the HUMAN IMMUNODEFICIENCY VIRUS.
The domestic cat, Felis catus, of the carnivore family FELIDAE, comprising over 30 different breeds. The domestic cat is descended primarily from the wild cat of Africa and extreme southwestern Asia. Though probably present in towns in Palestine as long ago as 7000 years, actual domestication occurred in Egypt about 4000 years ago. (From Walker's Mammals of the World, 6th ed, p801)
Proteins prepared by recombinant DNA technology.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Spherical RNA viruses, in the order NIDOVIRALES, infecting a wide range of animals including humans. Transmission is by fecal-oral and respiratory routes. Mechanical transmission is also common. There are two genera: CORONAVIRUS and TOROVIRUS.
The medical science concerned with the prevention, diagnosis, and treatment of diseases in animals.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
A species of CORONAVIRUS causing a fatal disease to pigs under 3 weeks old.
Component of the NATIONAL INSTITUTES OF HEALTH. It conducts and supports basic biomedical research that is not targeted to specific diseases and funds studies on genes, proteins, and cells, as well as on fundamental processes like communication within and between cells and metabolism. It was established in 1962.

Crystal structures of two H-2Db/glycopeptide complexes suggest a molecular basis for CTL cross-reactivity. (1/841)

Two synthetic O-GlcNAc-bearing peptides that elicit H-2Db-restricted glycopeptide-specific cytotoxic T cells (CTL) have been shown to display nonreciprocal patterns of cross-reactivity. Here, we present the crystal structures of the H-2Db glycopeptide complexes to 2.85 A resolution or better. In both cases, the glycan is solvent exposed and available for direct recognition by the T cell receptor (TCR). We have modeled the complex formed between the MHC-glycopeptide complexes and their respective TCRs, showing that a single saccharide residue can be accommodated in the standard TCR-MHC geometry. The models also reveal a possible molecular basis for the observed cross-reactivity patterns of the CTL clones, which appear to be influenced by the length of the CDR3 loop and the nature of the immunizing ligand.  (+info)

Interferon-induced human MxA GTPase blocks nuclear import of Thogoto virus nucleocapsids. (2/841)

Interferon-induced human MxA protein belongs to the dynamin superfamily of large GTPases. It exhibits antiviral activity against a variety of RNA viruses, including Thogoto virus, an influenza virus-like orthomyxovirus transmitted by ticks. Here, we report that MxA blocks the transport of Thogoto virus nucleocapsids into the nucleus, thereby preventing transcription of the viral genome. This interaction can be abolished by a mAb that neutralizes the antiviral activity of MxA. Our results reveal an antiviral mechanism whereby an interferon-induced protein traps the incoming virus and interferes with proper transport of the viral genome to its ultimate target compartment within the infected cell.  (+info)

DNA vaccination with hantavirus M segment elicits neutralizing antibodies and protects against seoul virus infection. (3/841)

Seoul virus (SEOV) is one of four known hantaviruses causing hemorrhagic fever with renal syndrome (HFRS). Candidate naked DNA vaccines for HFRS were constructed by subcloning cDNA representing the medium (M; encoding the G1 and G2 glycoproteins) or small (S; encoding the nucleocapsid protein) genome segment of SEOV into the DNA expression vector pWRG7077. We vaccinated BALB/c mice with three doses of the M or S DNA vaccine at 4-week intervals by either gene gun inoculation of the epidermis or needle inoculation into the gastrocnemius muscle. Both routes of vaccination resulted in antibody responses as measured by ELISA; however, gene gun inoculation elicited a higher frequency of seroconversion and higher levels of antibodies in individual mice. We vaccinated Syrian hamsters with the M or S construct using the gene gun and found hantavirus-specific antibodies in five of five and four of five hamsters, respectively. Animals vaccinated with the M construct developed a neutralizing antibody response that was greatly enhanced in the presence of guinea pig complement. Immunized hamsters were challenged with SEOV and, after 28 days, were monitored for evidence of infection. Hamsters vaccinated with M were protected from infection, but hamsters vaccinated with S were not protected.  (+info)

The effect of template RNA structure on elongation by HIV-1 reverse transcriptase. (4/841)

Reverse transcription of the RNA genome of retroviruses has to proceed through some highly structured regions of the template. The RNA genome of the human immunodeficiency virus type 1 (HIV-1) contains two hairpin structures within the repeat (R) region at the 5' end of the viral RNA (Fig. 1Fig. 1Template RNA structure of the HIV-1 R region and the position of reverse transcription pause sites. The HIV-1 R region (nucleotides +1/97) encodes two stable RNA structures, the TAR and polyA hairpins [5]. The latter hairpin contains the AAUAAA hexamer motif (marked by a box) that is involved in polyadenylation. The lower panel shows the predicted structures of the wild-type and two mutant forms of the polyA hairpin that were used in this study. Nucleotide substitutions are boxed, deletions are indicated by black triangle. The thermodynamic stability (free energy or DeltaG, in kcal/mol) was calculated according to the Zucker algorithm [71]. The TAR hairpin has a DeltaG of -24.8 kcal/mol. Minus-strand DNA synthesis on these templates was initiated by a DNA primer annealed to the downstream PBS. The position of reverse transcription pause sites observed in this study are summarized. All numbers refer to nucleotide positions on the wild-type HIV-1 transcript. Filled arrows represent stops observed on the wild-type template, and open arrows mark the pause sites that are specific for the structured A-mutant template. The sizes of the arrows correspond to the relative frequency of pausing. Little pausing was observed on the B-mutant template with the destabilized polyA hairpin.). These structures, the TAR and polyA hairpins, fulfil important functions in the viral life cycle. We analyzed the in vitro elongation properties of the HIV-1 reverse transcriptase (RT) enzyme on the wild-type RNA template and mutants thereof with either a stabilized or a destabilized polyA hairpin. Stable RNA structure was found to interfere with efficient elongation of the RT enzyme, as judged by the appearance of pause cDNA products. A direct relation was measured between the stability of template RNA structure and the extent of RT pausing. However, the position of structure-induced pause sites is rather diverse, with significant stops at a position approximately 6 nt ahead of the basepaired stem of the TAR and polyA hairpins. This suggests that the RT enzyme is stalled when its most forward domain contacts the RNA duplex. Addition of the viral nucleocapsid protein (NC) to the in vitro assay was found to overcome such structure-induced RT stops. These results indicate that the RT polymerase has problems penetrating regions of the template with stable RNA structure. This effect was more pronounced at high Mg2+ concentrations, which is known to stabilize RNA secondary structure. Such a structure-induced defect was not apparent in reverse transcription assays performed in virus-infected cells, which is either caused by the NC protein or other components of the virion particle. Thus, retroviruses can use relatively stable RNA structures to control different steps in the viral life cycle without interfering with the process of reverse transcription.  (+info)

The nucleocapsid protein of murine hepatitis virus type 3 induces transcription of the novel fgl2 prothrombinase gene. (5/841)

Using a set of parental and recombinant murine hepatitis virus strains, we demonstrate that the nucleocapsid protein induces transcription of the novel fgl2 prothrombinase gene and elevated procoagulant activity in those strains that produce fulminant hepatitis. Chinese hamster ovary cells cotransfected with a construct expressing nucleocapsid protein from susceptible strains and with a luciferase reporter construct containing the fgl2 promoter showed a 6-fold increase in luciferase activity compared with nontransfected cells or cells cotransfected with a construct expressing nucleocapsid protein from resistant strains. Two deletions found at coding sites 111-123 and 1143-1145 of structural domains I and III, respectively, of the nucleocapsid gene may account for the differences between pathogenic and nonpathogenic strains. Preliminary mapping of the fgl2 promoter has defined a region from -372 to -306 upstream from the ATG translation initiation site to be responsive to nucleocapsid protein. Hence, mapping of genetic determinants in parental and recombinant strains demonstrates that the nucleocapsid protein of strains that induce fulminant hepatitis is responsible for transcription of the fgl2 prothrombinase gene. These studies provide new insights into the role of the nucleocapsid gene in the pathogenesis of viral hepatitis.  (+info)

Comparative analysis of evolutionary mechanisms of the hemagglutinin and three internal protein genes of influenza B virus: multiple cocirculating lineages and frequent reassortment of the NP, M, and NS genes. (6/841)

Phylogenetic profiles of the genes coding for the hemagglutinin (HA) protein, nucleoprotein (NP), matrix (M) protein, and nonstructural (NS) proteins of influenza B viruses isolated from 1940 to 1998 were analyzed in a parallel manner in order to understand the evolutionary mechanisms of these viruses. Unlike human influenza A (H3N2) viruses, the evolutionary pathways of all four genes of recent influenza B viruses revealed similar patterns of genetic divergence into two major lineages. Although evolutionary rates of the HA, NP, M, and NS genes of influenza B viruses were estimated to be generally lower than those of human influenza A viruses, genes of influenza B viruses demonstrated complex phylogenetic patterns, indicating alternative mechanisms for generation of virus variability. Topologies of the evolutionary trees of each gene were determined to be quite distinct from one another, showing that these genes were evolving in an independent manner. Furthermore, variable topologies were apparently the result of frequent genetic exchange among cocirculating epidemic viruses. Evolutionary analysis done in the present study provided further evidence for cocirculation of multiple lineages as well as sequestering and reemergence of phylogenetic lineages of the internal genes. In addition, comparison of deduced amino acid sequences revealed a novel amino acid deletion in the HA1 domain of the HA protein of recent isolates from 1998 belonging to the B/Yamagata/16/88-like lineage. It thus became apparent that, despite lower evolutionary rates, influenza B viruses were able to generate genetic diversity among circulating viruses through a combination of evolutionary mechanisms involving cocirculating lineages and genetic reassortment by which new variants with distinct gene constellations emerged.  (+info)

Production, characterization, and uses of monoclonal antibodies against recombinant nucleoprotein of elk coronavirus. (7/841)

This is the first report of the production of monoclonal antibodies against elk coronavirus. The nucleoprotein gene of elk coronavirus was amplified by PCR and was cloned and expressed in a prokaryotic expression vector. Recombinant nucleocapsid protein was used to immunize mice for the production of hybridomas. Twelve hybridomas that produced monoclonal antibodies against the nucleocapsid protein of elk coronavirus were selected by an indirect fluorescent-antibody test, an enzyme-linked immunosorbent assay, and a Western blot assay. Ten of the monoclonal antibodies were of the immunoglobulin G1 (IgG1) isotype, one was IgG2a, and one was IgM. All had kappa light chains. By immunohistochemistry four monoclonal antibodies detected bovine coronavirus and elk coronavirus in formalin-fixed intestinal tissues. Antinucleoprotein monoclonal antibodies were found to be better at ruminant coronavirus detection than the anti-spike protein monoclonal antibodies. Because nucleoprotein is a more abundant antigen than spike protein in infected cells, this was not an unexpected finding.  (+info)

Recognition of an MHC class I-restricted antigenic peptide can be modulated by para-substitution of its buried tyrosine residues in a TCR-specific manner. (8/841)

Conformational dependence of TCR contact residues of the H-2Kb molecule on the two buried tyrosine side chains of the vesicular stomatitis virus (VSV)-8 peptide was investigated by systematic substitutions of the tyrosines with phenylalanine, p-fluorophenylalanine (pFF), or p-bromophenylalanine (pBrF). The results of peptide competition CTL assays revealed that all of the peptide variants, except for the pBrF analogues, had near-native binding to the H-2Kb molecule. Epitope-mapped anti-H-2Kb mAbs detected conformational differences among H-2Kb molecules stabilized with these VSV-8 variants on RMA-S cells. Selective recognition of the VSV-8 analogues was displayed by a panel of three H-2Kb-restricted, anti-VSV-8 TCRs. Thus, these substitutions result in an antigenically significant conformational change of the MHC molecular surface structure at both C and D pockets, and the effect of this change on cognate T cell recognition is dependent on the TCR structure. Our results confirm that the structure of buried peptide side chains can determine the surface conformation of the MHC molecule and demonstrate that even a very subtle structural nuance of the buried side chain can be incorporated into the surface conformation of the MHC molecule. The ability of buried residues to modulate this molecular surface augments the number of residues on the MHC-peptide complex that can be recognized as "foreign" by the CD8+ T cell repertoire and allows for a higher level of antigenic discrimination. This may be an important mechanism to expand the total number of TCR specificities that can respond to a single peptide determinant.  (+info)

Recombinant MERS-CoV Coronavirus Nucleocapsid Protein (Met1-Asp413) 40068-V08B with a fusion His Tag, is expressed in Baculovirus-Insect Cells. With high purity, high biological activity, high stability, and other superior features, you can use this MERS-CoV Coronavirus Nucleocapsid protein for relevant bioassay and related research.
We have professional and advanced research and production capacity for Coronavirus Nucleocapsid reagents production, including Proteins, Antibodies, cDNA Clones, ELISA Kits,etc. All NP-CoV products are produced in house and quality controlled.
IF: 4.606] GENOME REPLICATION AND REGULATION OF VIRAL GENE EXPRESSION: Porcine Reproductive and Respiratory Syndrome Virus Nucleocapsid Protein Interacts with Nsp9 and Cellular DHX9 To Regulate Viral RNA ...
The authors show the highly homologous N proteins from SARS-CoV, SARS-CoV2 and MERS interact with MASP-2. MASP-2 is a protease critical to complement activation by the lectin pathway. In an in vivo murine infection model the N protein of SARS-CoV expressed from an adenoviral vector enhances LPS induced lung injury; a phenotype lost by blocking N protein: MASP2 interaction and by suppressing complement activation. Serum C5a levels were significantly increased in severe COVID-19 patients. 2 of these COVID-19 patients treated with recombinant anti-C5a antibody show clinical improvement. This indicates that targeting complement pathways offers therapeutic potential for pneumonia induced by coronaviruses. ...
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. ...
Publications related to biomolecular condensates, phase separation, llps and more. Tightly packed complexes of nucleocapsid protein and genomic RNA form the core of viruses and may assemble within viral factories, dynamic compartments formed within the host cells. Here, we examine the possibility that the multivalent RNA-binding nucleocapsid protein (N) from the severe acute respiratory syndrome coronavirus (SARS-CoV-2) compacts RNA via protein-RNA liquid-liquid phase separation (LLPS) and that N interactions with host RNA-binding proteins are mediated by phase separation. To this end, we created a construct expressing recombinant N fused to a N-terminal maltose binding protein tag which helps keep the oligomeric N soluble for purification. Using in vitro phase separation assays, we find that N is assembly-prone and phase separates avidly. Phase separation is modulated by addition of RNA and changes in pH and is disfavored at high concentrations of salt. Furthermore, N enters into in vitro phase
SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) Monoclonal Antibody IgG2b. Purified mouse monoclonal antibody (IgG2b) raised against recombinant Nucleocapsid Protein (NP) from SARS-CoV-2 (COVID-19).. Concentration: ≥1 μg/μl. Clonality: Monoclonal. Clone: 1C7. ...
Rabbit polyclonal H1N1 Influenza A virus Nucleocapsid protein antibody validated for WB, ELISA, ICC/IF and tested in InfA. Immunogen corresponding to synthetic…
SARS Nucleocapsid Protein Antibodies available through Novus Biologicals. Browse our SARS Nucleocapsid Protein Antibody catalog backed by our Guarantee+.
HIV nucleocapsid protein. Molecular model of the nucleocapsid protein (blue) from HIV-1 (human immunodeficiency virus-type 1) complexed with the Psi RNA (ribonucleic acid) packaging element (pink). The Psi element is involved in the packaging of viral RNA into the capsid during replication. - Stock Image C025/1461
Recombinant DNA techniques were used to delete regions of a cDNA clone of the phosphoprotein NS gene of vesicular stomatitis virus. The complete NS gene and four mutant genes containing internal or terminal deletions were inserted into a modified pGem4 vector under the transcriptional control of the phage T7 promoter. Run-off transcripts were synthesized and translated in vitro to provide [35S]methionine-labeled complete NS or deletion mutant NS proteins. Immune coprecipitation assays involving these proteins were developed to map the regions of the NS protein responsible for binding to the structural viral nucleocapsid protein N and the catalytic RNA polymerase protein L. The data indicate the NS protein is a bivalent protein consisting of two discrete functional domains. Contrary to previous suggestions, the negatively charged amino-terminal half of NS protein binds to L protein, while the carboxyl-terminal half of NS protein binds to both soluble recombinant nucleocapsid protein N and viral ...
Recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein is produced by our E.coli expression system and the target gene encoding Met1-Ala419 is expressed with a 6His tag at the N-terminus. Bon Opus Cat.# DRA31
Recombinant SARS-CoV-2 Nucleocapsid (N) Protein - Cat. No. 10-067 | ProSci can be used to detect Virus Recombinant SARS-CoV-2, Nucleocapsid (N) Protein - 100 µg in ELISA, WB and other applications.
Scientists have uncovered the first full structure of the coronavirus nucleocapsid (N) protein and discovered how antibodies from COVID-19 patients interact with it. They also determined that the structure appears similar across many coronaviruses, including recent COVID-19 variants - making it an ideal target for advanced treatments and vaccines.
The conserved N-terminal region of Sendai virus nucleocapsid protein NP is required for nucleocapsid assemblyBUCHHOLZ, C. J; SPEHNER, D; DRILLIEN, R et al.Journal of virology. 1993, Vol 67, Num 10, pp 5803-5812, issn 0022-538XArticle ...
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Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
DNA sequence encoding the COVID-19 Nucleocapsid protein domain ,amino acids[1-419] (accession# YP_009724397.2) including a C-terminal His tag was expressed in CHO Cells.
Bonjour mon ordi est infecté par le virus N039. Voici le rapport dhijackthis: Logfile of Trend Micro HijackThis v2.0.0 (BETA) Scan saved at 14:46:31, on 02/10/2007 Platform: Windows XP SP2 (WinN
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2/COVID-19) Nucleocapsid Protein (NP) Antibody is a Mouse Monoclonal Antibody against SARS-CoV-2/COVID-19 NP. |p||/p| The SARS-CoV-2 Nucleoprotein (also known as Nucleocapsid Protein or
Summary Antigenic and structural variation in the major nucleocapsid protein, VPN41, from different strains of respiratory syncytial (RS) virus was observed using a combination of monoclonal antibodies and two-dimensional peptide mapping. Limited trypsin treatment of intact nucleocapsids produced two peptide fragments M r 27K and M r 14K. Two monoclonal antibodies, N1 and N2, reactive with primary sequence epitopes located on intact nucleocapsids also reacted with either the 27K fragment (N2) or the 14K fragment (N1). Competitive radioimmunoassay studies using N1 and N2 antibodies revealed two discrete antigenic groups among the seven human strains of RS virus examined. A bovine strain of RS virus, although antigenically similar to the human strain of RS virus, was placed in a separate group. Two-dimensional peptide mapping of 125I-labelled VPN41 purified by SDS-PAGE revealed extensive structural homology between all strains. However, several unique tryptic/chymotryptic peptides supported the grouping
Gentaur molecular products has all kinds of products like :search , GenWay \ Hepatitis C Virus Nucleocapsid Genotype 6a - N_A N_A \ 10-663-45387 for more molecular products just contact us
A Sandwich ELISA kit to Quantitatively measure Nucleocapsid protein in bronchoalveolar lavage fluid and nasopharyngeal swab samples
1DSQ: The NMR structure of the nucleocapsid protein from the mouse mammary tumor virus reveals unusual folding of the C-terminal zinc knuckle.
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Viral Antigen ID: Performed on specimens obtained via nasopharyngeal or nasal swabs.. The test methodology is a well-established, widely used Fluorescent Immunoassay (FIA), provided under FDA-granted EUA for the detection and identification of specific protein structures associated with SARS-CoV-2, namely: the spike protein, and the nucleocapsid protein (both known as viral antigens), is highly specific for confirming COVID-19. The FDA has authorized one. (1) testing system EUA in determining the presence of SARS-Co-V-2 Antigen. That test, ...
Purified Recombinant COVID-19 N protein truncated N04, His-tagged from Creative Biomart. Recombinant COVID-19 N protein truncated N04, His-tagged can be used for research.
SARS-CoV-2 N protein ELISA Kit from Proteintech (96 tests) . Quantitate SARS-CoV-2 N protein in Serum, Plasma, Cell culture supernatant, Urine. Cat.No. KE30007.
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
INgezim COVID 19 DR The INgezim COVID assays use the SARS-CoV-2 nucleoprotein (N protein) as the antigen for the detection of antibodies to SARS-CoV-2. The N protein is one of the most abundant early-onset proteins of the virus.
Shi, Y.; Yi, Y.; Li, P.; Kuang, T.; Li, L.; Dong, M.; Ma, Q.; Cao, C., 2003: Diagnosis of severe acute respiratory syndrome (SARS) by detection of SARS coronavirus nucleocapsid antibodies in an antigen-capturing enzyme-linked immunosorbent assay
TY - JOUR. T1 - TBK1 mediates critical effects of measles virus nucleocapsid protein (MVNP) on pagetic osteoclast formation. AU - Sun, Quanhong. AU - Sammut, Bénédicte. AU - Wang, Feng Ming. AU - Kurihara, Noriyoshi. AU - Windle, Jolene J.. AU - Roodman, G. David. AU - Galson, Deborah L.. PY - 2014/1. Y1 - 2014/1. N2 - Pagets disease of bone (PDB) is characterized by abnormal osteoclasts with unique characteristics that include increased sensitivity of osteoclast progenitors to 1,25(OH)2D3, receptor activator of NF-κB ligand (RANKL), and TNF-α; increased osteoclast numbers; and increased expression of IL-6 and several transcription factors. We recently reported that measles virus nucleocapsid protein (MVNP) plays a key role in the development of these abnormal osteoclasts. MVNP can induce the pagetic osteoclast phenotype in vitro and in vivo in TRAP-MVNP transgenic mice. However, the molecular mechanisms by which MVNP generates pagetic osteoclasts have not been determined. TANK-binding ...
Porcine reproductive and respiratory syndrome virus (PRRSV) is an arterivirus responsible for a widespread contagious disease of domestic pigs with high economic impact. Switzerland is one of the rare PRRSV-free countries in Europe, although sporadic outbreaks have occurred in the past. The PRRSV isolate IVI-1173 from the short outbreak in Switzerland in 2012 was entirely sequenced, and a functional full-length cDNA clone was constructed. Genetic and antigenic characterization of IVI-1173 revealed the importance of amino acid 90 of the nucleocapsid protein N as part of a conformational epitope. IVI-1173 was not detected by SDOW17, a monoclonal antibody against N widely used to detect PRRSV-infected cells. Substitution of alanine at position 90 of N [N(A90)] with a threonine [N(T90)] restored reactivity of vIVI1173-N(T90) to SDOW17 completely. The relevance of this amino acid for the conformational SDOW17 epitope of PRRSV N was further confirmed by the opposite substitution in a functional cDNA clone of
The details of the mechanism by which severe acute respiratory syndrome-associated coronavirus (SARS-CoV) causes severe pneumonia are unclear. We investigated the immune responses and pathologies of SARS-CoV-infected BALB/c mice that were immunized intradermally with recombinant vaccinia virus (VV) …
Fingerprint Dive into the research topics of Targeted RNA recombination of the membrane and nucleocapsid protein genes between mouse hepatitis virus and bovine coronavirus.. Together they form a unique fingerprint. ...
Coronaviruslike viruses previously have been identified in feces of diarrheic foals and adult horses (1, 7, 8, 13, 17). In the present study, a coronavirus associated with diarrhea in a young foal was serially propagated in cell culture and partially characterized. The virus was identified as a coronavirus based on (i) virion size and morphology, (ii) antigenic relatedness to BCV and HEV as determined by serological procedures, and (iii) genetic relatedness to BCV, HCV strain OC43, and MHV as determined by N gene sequence analysis. The virus tentatively is identified as equine coronavirus (ECV) based on the origin of the virus.. The coronavirus N protein has been shown to be highly variable in amino acid composition between the viruses that comprise the three coronavirus antigenic groups but highly conserved within these groups (27, 30). In the present study, a high degree of identity (66.7 to 90.1%) was observed between the N protein sequences of ECV strain NC99 and N protein sequences of group ...
Hong Kong Med J 2009;15(Suppl 2):S33-6 Mouse studies of SARS coronavirus-specific immune responses to recombinant replication-defective adenovirus expressing SARS coronavirus N protein FWY Sin, SCS Ch
Serum samples from 317 patients with patients with severe acute respiratory syndrome (SARS) were tested for the nucleocapsid (N) protein of SARS-associated coronavirus, with sensitivities of 94% and 78% for the first 5 days and 6-10 days after onset, respectively. The specificity was 99.9%. N protein can be used as an early diagnostic maker for SARS ...
TY - JOUR. T1 - Nucleocapsid and glycoprotein organization in an enveloped virus. AU - Holland Cheng, R.. AU - Kuhn, Richard J.. AU - Olson, Norman H.. AU - Rossmann^Hok-Kin Choi, Michael G.. AU - Smith, Thomas J.. AU - Baker, Timothy S.. PY - 1995/2/24. Y1 - 1995/2/24. N2 - Alphaviruses are a group of icosahedral, positive-strand RNA, enveloped viruses. The membrane bilayer, which surrounds the ∼ 400 Å diameter nucleocapsid, is penetrated by 80 spikes arranged in a T = 4 lattice. Each spike is a trimer of heterodimers consisting of glycoproteins Ell and E2. Cryoelectron microscopy and image reconstruction of Ross River virus showed that the T=4 quaternary structure of the nucleocapsid consists of pentamerand hexamer clusters of the capsid protein, but not dimers, as have been observed in several crystallographic studies. The E1-E2 heterodimers form one-to-one associations with the nucleocapsid monomers across the lipid bilayer. Knowledge of the atomic structure of the capsid protein and our ...
Porcine deltacoronavirus (PDCoV) is a newly detected porcine coronavirus causing serious vomiting and diarrhea in piglets, especially newborn piglets. There has been an outbreak of PDCoV in worldwide since 2014, causing significant economic losses in the pig industry. The interferon (IFN)-mediated antiviral response is an important component of virus-host interactions and plays an essential role in inhibiting virus infection. However, the mechanism of PDCoV escaping the porcine immune surveillance is unclear. In the present study, we demonstrated that the PDCoV nucleocapsid (N) protein antagonizes porcine IFN-β production after vesicular stomatitis virus (VSV) infection or poly(I:C) stimulation. PDCoV N protein also suppressed the activation of porcine IFN-β promoter when it was stimulated by porcine RLR signaling molecules. PDCoV N protein targeted porcine retinoic acid-inducible gene I (pRIG-I) and porcine TNF receptor associated factor 3 (pTRAF3) by directly interacting with them. The N-terminal
For two members of the order Mononegavirales, i.e., vesicular stomatitis virus (VSV), a rhabdovirus, and RSV, a paramyxovirus, it has been determined that the L polymerase is capable of RNA synthesis in vitro, using naked RNA as a substrate, with L initiating RNA synthesis de novo at the 3′ terminus of the leader (Le) RNA (40, 43). For VSV, it was also demonstrated that P serves as a processivity factor for L, even with naked RNA, and that full processivity of the P-L heterocomplex additionally requires the template-associated N protein (43). In living cells, the genomic RNA of RSV is not naked but wrapped by the N protein, forming an N-RNA complex in which RNA is sequestered and hidden. Reading of the RNA sequence by the polymerase requires (i) specific targeting to this template, which is mediated by the interaction between the nucleocapsid and the C terminus of P (31), and (ii) the local opening of the N-RNA complex, since RNA is sequestered in a basic groove located between the two ...
Genome packaging is a key step in retrovirus replication. Two copies of the virus RNA are specifically selected and packaged into an assembling virion. The nucleocapsid (NC) domain of the Gag polyprotein and the packaging signal (?-site) region of the genomic RNA are critical to this process. Different packaging mechanisms have been proposed for HIV-1 and HIV-2. In HIV-1, the ?-site is downstream of the major splice donor (SD), and any splicing removes both NC binding sites. The proposed HIV-2 ?-site is upstream of the SD. A co-translational packaging hypothesis was presented explaining this contradiction. In this mechanism, NC binds the full-length RNA from which it was translated. Other studies indicated the region between the SD and the Gag initiation codon is critical for NC binding and genome packaging. This finding supports the hypothesis that HIV-1 and HIV-2 package their genomes in a similar manner. However, the minimal NC binding domain(s) in HIV-2 had not been determined. The work ...
Profiles of antibodies towards the nucleocapsid proteins from the severe acute respiratory symptoms (SARS)-associated coronavirus in 445 possible SARS sufferers and 3,749 healthy people or non-SARS sufferers were analyzed by antigen-capturing enzyme-linked immunosorbent assay. and non-SARS sufferers, just seven (0.187%) were weakly positive. The novel serious acute respiratory symptoms (SARS)-linked coronavirus (CoV) continues to be defined as the etiologic Rabbit polyclonal to MAP1LC3A. agent of SARS (1, 3, 5). Its been confirmed that, at least in early replies, the antibodies towards the nucleocapsid proteins (N proteins) predominate, as assayed by Traditional western blotting and proteomic evaluation. To comprehend the humoral immunity towards the N proteins of SARS CoV and the chance of using the N proteins in MP470 SARS medical diagnosis, antibodies towards the N proteins from 445 sufferers who acquired SARS most likely, as diagnosed based on World Health Firm requirements, from four ...
CD83 was first described for Langerhans cells and activated lymphocytes. It is a conserved member of the immunoglobulin superfamily and is also known as one of the most characteristic cell surface markers for fully mature DCs in peripheral circulation (31, 50, 51). Although CD83 plays essential roles in both the central and peripheral immune systems, the underlying mechanisms by which CD83 regulates immune responses remain enigmatic. Two different protein isoforms of CD83 have been reported in vivo: a membrane-bound form (mCD83) and a soluble form (sCD83). Enhancement of CD83 expression inhibits the surface expression of other molecules and influences DC function by dramatically reducing T cell activation, underlining and confirming the critical function of mCD83 (51). sCD83 is a potent inhibitor of anti-CD3-induced proliferation of T cells and production of IL-2 and IFN-γ by PBMCs, but the molecular mechanism by which CD83 suppresses the immune system is not understood (52-54). Virus-mediated ...
Structure of the equine arteritis virus nucleocapsid protein reveals a dimer-dimer arrangement. Acta Crystallographica Section D: Biological Crystallography. 63:581-586. 2007 ...
The FORA COVID-19 Antigen Rapid Test is a lateral flow chromatographic immunoassay for the qualitative detection of nucleocapsid protein antigen from SARS-CoV-2 in fresh swab specimens collected directly from the nose and throat region of persons in whom caregivers suspect they have COVID-19. The FORA COVID-19 Antigen
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Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. Nucleocapsid assembly is concommitant with replication, therefore viral replication depends on the intracellular concentration of free N, termed N(0). All replicative products are resistant to nucleases.
Other ORFs on the one-third of the genome near the 3-terminus encodes at least four main structural protein: spike (S), membrane (M), envelope (E) and nucleocapsid (N) proteins. Besides these four main structural proteins, such as HE protein, 3a/b protein, and 4a/b protein. All the structural and accessory proteins are translated from the from the…
Retrotransposon TYA Gag And TYB Pol Genes; Transcribed/translated As One Unit; Polyprotein Is Processed To Make A Nucleocapsid-like Protein (Gag), Reverse Transcriptase (RT), Protease (PR), And Integrase (IN); Similar To Retroviral Genes
The nucleocapsid consisting of a protein shell and viral nucleic acids has a helical symmetry. Nucleocapsids have a diameter of ... M1 - Matrix protein. Facilitates nucleocapsid and envelope interactions. M2-1 - Matrix protein. Intragenic and intergenic ... N - Nucleocapsid protein. Essential for viral replication and transcription. Plays a major role in forming a capsid around the ... Then, through the action of the fusion protein, the virus fuses to the host plasma membrane and the nucleocapsid is released. ...
Gag encodes a polyprotein with a capsid and a nucleocapsid domain. Gag proteins form virus-like particles in the cytoplasm ... Sandmeyer, Suzanne B; Clemens, Kristina A (2010). "Function of a retrotransposon nucleocapsid protein". RNA Biology. 7 (6): 642 ... Based on structure, presence/absence of specific protein domains, and conserved protein sequence motifs, they can be subdivided ... Occasional ribosomal frameshifting allows the production of both proteins, while ensuring that much more Gag protein is ...
Another important structural protein is the phosphoprotein N, which is responsible for the helical symmetry of the nucleocapsid ... McBride R, van Zyl M, Fielding BC (August 2014). "The coronavirus nucleocapsid is a multifunctional protein". Viruses. 6 (8): ... They are enveloped viruses with a positive-sense single-stranded RNA genome and a nucleocapsid of helical symmetry. The genome ... protein species, S and M. Glycoprotein S comprises the large surface projections, while M is a triple-spanning transmembrane ...
Inside the envelope, there is the nucleocapsid, which is formed from multiple copies of the nucleocapsid (N) protein, which are ... There, the M proteins direct most protein-protein interactions required for assembly of viruses following its binding to the ... and nucleocapsid. The later ORFs also encode for eight unique proteins (orf3a to orf9b), known as the accessory proteins, many ... Chang CK, Hou MH, Chang CF, Hsiao CD, Huang TH (March 2014). "The SARS coronavirus nucleocapsid protein--forms and functions". ...
Nevertheless, viral particles have been found to have a nucleocapsid. The source of the nucleocapsid protein remains unknown. ... proteins. GB-C virus does not appear to encode a C (core or nucleocapsid) protein like, for instance, hepatitis C virus. ... About 2% of healthy US blood donors are viraemic with GBV-C, and up to 13% of blood donors have antibodies to E2 protein, ... This beneficial effect may be related to action of several GBV-C viral proteins, including NS5A phosphoprotein and E2 envelope ...
ORF6 encodes the membrane protein. The nucleocapsid (N) protein is encoded by ORF7. The N protein is composed of 123 amino ... At the end of replication, the nucleocapsid proteins surround the newly made genome. The new nucleocapsid complex buds from the ... When researchers knocked out DHX36, the activation of NF-κB signaling by PRSSV and nucleocapsid (N) protein was inhibited. ... ORF2a, 3, 4, 5, encode glycoprotein 2,2a, 3, 4, and 5. ORF2b encodes the envelope protein. There is a newly discovered protein ...
FDV is not enveloped but has a nucleocapsid. The capsid has two layers and is icosahedral. The two known cell surface proteins ... There are at least six structural proteins coded for in the genome. Replication occurs in the host cell cytoplasm or ...
Typical nucleoproteins include ribosomes, nucleosomes and viral nucleocapsid proteins. Nucleoproteins tend to be positively ... Bank, RCSB Protein Data. "RCSB Protein Data Bank - RCSB PDB". Archived from the original on 2015-04-18. Retrieved 2018-04-14. ... DNA-binding protein RNA-binding protein Nucleoproteins at the US National Library of Medicine Medical Subject Headings (MeSH) ... Currently, over 2000 RNPs can be found in the RCSB Protein Data Bank (PDB). Furthermore, the Protein-RNA Interface Data Base ( ...
The HIV-1 nucleocapsid protein 7 (NCp7) is the protein targeted by zinc ejectors. NCp7 is initially formed as part of the gag ... HIV Agents That Selectively Target Retroviral Nucleocapsid Protein Zinc Fingers without Affecting Cellular Zinc Finger Proteins ... Musah, Rabi Ann (2004). "The HIV-1 Nucleocapsid Zinc Finger Protein as a Target of Antiretroviral Therapy" (PDF). Current ... the compounds interact with an 18-residue polypeptide on the N terminal zinc knuckle region of the HIV nucleocapsid protein ...
They also share similar capsid and nucleocapsid proteins/domains. Caulimoviruses also share some features with belpaoviruses, ... Their polymerase proteins are similar in structure and include aspartic protease (retroviral aspartyl protease) and an ... Their reverse transcriptase proteins share a common origin. Moreover, belpaoviruses, metaviruses, pseudoviruses, and ... "Homologous Capsid Proteins Testify to the Common Ancestry of Retroviruses, Caulimoviruses, Pseudoviruses, and Metaviruses". ...
L protein), the M RNA segment encodes two surface glycoproteins (Gc and Gn), and the S RNA segment encodes a nucleocapsid ... The N protein is the most abundant viral protein in Bunyaviridae virus particles and infected cells and, therefore, the main ... "The crystal structure of the Hazara virus nucleocapsid protein". BMC Structural Biology. 15: 24. doi:10.1186/s12900-015-0051-3 ... protein (N). The three genomic RNA segments are encapsidated by copies of the N protein in the form of ribonucleoprotein (RNP) ...
The nucleocapsid contains ~2000 copies of the capsid protein. The symmetry of the nucleocapsid is helical with a pitch of 3.4 ... capsid protein (CP) and two small putative proteins known as 6K1 and 6K2. The P3 cistron also encodes a second protein-P3N-PIPO ... The capsid protein ranges between 30 and 35 kDa in weight. The VPg protein interacts with eukaryotic initiation factor 4E ( ... end a protein is covalently linked (the Vg protein). It encodes a single open reading frame (ORF) expressed as a 350kDa ...
The structural proteins include capsid (C), premembrane/membrane (prM), and envelope (E). The non-structural proteins include ... A nucleocapsid is formed and takes up viral glycoproteins. However, very little is understood about the assembly process of ... of 3425 amino acid polyprotein that encodes three structural proteins and eight non-structural proteins. ... Protein 2K is not found in many other flaviviruses. NS3 functions as a protease and helicase. NS5 functions as the RNA- ...
The proteins making up the capsid are called capsid proteins or viral coat proteins (VCP). The capsid and inner genome is ... called the nucleocapsid. Capsids are broadly classified according to their structure. The majority of the viruses have capsids ... The bacteriophage encoded gp31 protein appears to be functionally homologous to E. coli chaparone protein GroES and able to ... The recruitment of cellular proteins appears to have occurred at different stages of evolution so that some cellular proteins ...
The virus is made up of four ssRNA segments, seven open reading frames, a nucleocapsid protein, and an RNA polymerase protein. ... RNA 3: Encodes for gene silencing suppressor proteins and nucleocapsid proteins. This table from KnowledgeBank.irri.org ... RNA 1 is negative sense and encodes a protein that is part of the RNA polymerase. RNA 2 and RNA 4: These strands are ambisense ... Xiong, Ruyi; Jianxiang Wu; Yijun Zhou; Xueping Zhou (2008). "Identification of a Movement Protein of the Tenuvirus Rice Stripe ...
FMV vcRNA3 (1490 nt) encodes a 35 kDa nucleocapsid (N) protein. FMV vcRNA4 (1472 nt) encodes a 40.5 kDa protein with function ... The protein encoded by the fourth segment on the viral genome, RNA4, still has an unknown function. Additionally, the exact ... Like all plant viruses, FMV encodes a movement protein (MP) to enable it to move between cells, usually by increasing the size ... FMV vcRNA 1 codes for RNA dependent RNA polymerase protein (RdRp), which has endonuclease function at its N terminus and is ...
These structures interact with amino acids located in the nucleocapsid, or NC domain, of the Gag protein. Once the ... Qualley DF, Sokolove VL, Ross JL (March 2015). "Bovine leukemia virus nucleocapsid protein is an efficient nucleic acid ... "Retrovirus-specific differences in matrix and nucleocapsid protein-nucleic acid interactions: implications for genomic RNA ... If the encapsidation (packaging) signal is not essential for this particle assembly, then the protein interactions among Gag ...
... segments which generally encode the nucleocapsid, envelope protein and the polymerase protein, respectively. The size of the S ... It has been demonstrated in previous studies that in mammalian cells, the NSs protein will induce a shut-off of host protein ... The S segment encodes the nucleocapsid (N) and the non-structural (NSs) proteins. The M segment encodes the virion surface ... "Bunyamwera virus possesses a distinct nucleocapsid protein to facilitate genome encapsidation". Proc Natl Acad Sci U S A. 110 ( ...
Levin JG, Mitra M, Mascarenhas A, Musier-Forsyth K. Role of HIV-1 nucleocapsid protein in HIV-1 reverse transcription. RNA Biol ... Levin, Judith G; Mitra, Mithun; Mascarenhas, Anjali; Musier-Forsyth, Karin (2010). "Role of HIV-1 nucleocapsid protein in HIV-1 ... Berkhout B, Mouland AJ, Musier-Forsyth K. Retroviral nucleocapsid protein and assembly. Foreword. Virus Res. 2014;193:1. ... biophysical and cell-based approaches to understand the interactions of proteins and RNAs involved in protein synthesis and ...
The major capsid protein VP39 together with some minor proteins forms the nucleocapsid (21 nm x 260 nm) that encloses the DNA ... The nucleocapsid assembly is not dependent on the Ac83 protein product. During periods of evolution, the baculoviral envelope ... This protein forms structures called peplomers on one end of the budded virus particle but is not found on ODV (although ... The protein matrix dissolves in the alkaline environment of the host midgut (stomach), releasing ODV that then fuse to the ...
The major coat proteins of both lipothrixviruses and rudiviruses have an unusual four-helix bundle topology. The genome is non- ... The virons are filamentous with a helical nucleocapsid. At either end are attached either fibers or more complex structures ... In both groups of viruses, the major capsid proteins form a claw-like dimer (homodimer in rudiviruses and heterodimer in ... Furthermore, whereas the capsid of rudiviruses is constructed from a single major capsid protein, that of lipothrixviruses is ...
"NMR structure of the HIV-1 nucleocapsid protein bound to stem-loop SL2 of the psi-RNA packaging signal. Implications for genome ... "Determination of the structure of the nucleocapsid protein NCp7 from the human immunodeficiency virus type 1 by 1H NMR". EMBO J ... "First glimpses at structure-function relationships of the nucleocapsid protein of retroviruses". Journal of Molecular Biology. ... Warui, DM; Baranger, AM (May 10, 2012). "Identification of small molecule inhibitors of the HIV-1 nucleocapsid-stem-loop 3 RNA ...
... haphazardly placed and usually involve scaffolding proteins in their formation around the nucleocapsid. Non-essential proteins ... is a cluster of proteins that lines the space between the envelope and nucleocapsid of all herpesviruses. The tegument ... "Herpes Simplex Virus Tegument Protein V1 Elucidation and Formation Around the Nucleocapsid". Journal of Virology. American ... These proteins are usually[citation needed] formed within the late phase of the viral infectious cycle, after viral genes have ...
HIV Tat protein, HIV TAR RNA, human CCR5 Antiviral. ALN-RSV01. RSV. RSV nucleocapsid ... RSV nucleocapsid Main challenge[edit]. As with conventional manufactured drugs, the main challenge in developing successful ... RNA has been largely investigated within its role as an intermediary in the translation of genes into proteins.[18] More active ... Such 3'-UTRs often contain both binding sites for microRNAs (miRNAs) as well as for regulatory proteins. By binding to specific ...
The nucleocapsid consists of a core of nucleic acid enclosed in a protein coat. Although they are categorized as negative-sense ... The S-segment RNA is approximately 3.5 kb, and encodes the viral nucleocapsid protein (NP) and glycoprotein (GPC). The L- ... Loureiro ME, D'Antuono A, Levingston Macleod JM, López N (September 2012). "Uncovering viral protein-protein interactions and ... and a small RING-domain containing protein (Z). The Z protein forms homo oligomers and a structural component of the virions. ...
The virions contain a thin, dense central nucleocapsid that encases the DNA-protein core. The nucleocapsid core is surrounded ... The nucleocapsids exit the nucleus into the cytoplasm through the nuclear pore complex, after which they associate with the ... protein composition, morphogenesis and cytopathology of Glossina pallidipes salivary gland hypertrophy virus". Journal of ... outer surface of the virions is studded with helical polymeric structure composed of virally-encoded and host-derived protein ...
... that is coded by the nucleocapsid protein, and the L polymerase function together to create a replication of the viral genome. ... "Diagnosis of Oropouche Virus Infection Using a Recombinant Nucleocapsid Protein-Based Enzyme Immunoassay". Journal of Clinical ... Through phylogenetic analysis of nucleocapsid genes in different oropouche virus strains, it has been revealed that there are ... These segments function to encode nucleocapsids, glycoproteins and the RNA polymerase in that sequential order. ...
... and a nonstructural protein (NSm), while the S RNA segment encodes a nucleocapsid protein (N) and, in an alternative ... The N protein is the most abundant protein in virus particles and infected cells and, therefore, the main target in many ... "Nucleocapsid protein structures from orthobunyaviruses reveal insight into ribonucleoprotein architecture and RNA ... The genomic RNA segments are encapsidated by copies of the N protein in the form of ribonucleoprotein (RNP) complexes. ...
"Localization of a T-cell epitope within the nucleocapsid protein of avian coronavirus". Immunology. 74 (1): 8-13. ISSN 0019- ... These particles contain Gag protein and HIV-Env surface protein. These particles can be recognized by B cells and subsequently ... Activities of Env-Specific Human Monoclonal Antibodies Generated from Volunteers Receiving the DNA Prime-Protein Boost HIV ...
The envelope proteins form small projections ~5-10 nm long. The nucleocapsids are filamentous and circular with a length of 200 ... October 2012). "Structure, function, and evolution of the Crimean-Congo hemorrhagic fever virus nucleocapsid protein". Journal ... and the S segment encodes the nucleocapsid protein. The mutation rates for the three parts of the genome were estimated to be: ... The virus might enter a cell using the cell surface protein nucleolin. The genome is circular, negative sense RNA in three ...
... the virus to bind to cellular proteins enabling it to fuse with internal cellular membranes and release the viral nucleocapsid. ... which code for proteins with antiviral properties.[51] EBOV's V24 protein blocks the production of these antiviral proteins by ... which are then translated into structural and nonstructural proteins. The most abundant protein produced is the nucleoprotein, ... EBOV replication overwhelms protein synthesis of infected cells and the host immune defences. The GP forms a trimeric complex, ...
... proteins - protocol - protozoa - provirus - pruritus - pseudo-Cushing's syndrome - pseudovirion - PUBMED - pulmonary - purified ... nucleocapsid - nucleoli - nucleoside - nucleoside analog - nucleoside reverse transcriptase inhibitors (NRTI) - nucleotide - ... core protein - correlates of immunity/correlates of protection - creatinine - cross-resistance - cryotherapy - cryptococcal ...
The virus consists of four nonstructural proteins and three structural proteins.[12] The structural proteins are the capsid and ... dissociates from E2 and initiates membrane fusion that allows the release of nucleocapsids into the host cytoplasm, promoting ... monocyte chemoattractant protein 1 (MCP-1), monokine induced by gamma interferon (MIG), and interferon gamma-induced protein 10 ... a nonstructural protein that degrades RBP1 and turns off the host cell's ability to transcribe DNA.[51] NS2 interferes with the ...
Envelope fusion with the plasma membrane of the host cell causes separation of the nucleocapsid from viral DNA and proteins. ... DNA and proteins enter the host cell nucleus and turn-off host cell synthesis of nucleic acids, proteins, and other macro ... Similar Alphaviruses use gC protein to aid in binding the virion to the cell and gD to stabilize it, if required. gB, gD, gH, ... L genes are transcribed "after the synthesis of DNA and viral protein onset". Virion DNA maturation occurs as the nucleocapids ...
In fission yeast this complex contains argonaute, a chromodomain protein Chp1, and a protein called Tas3 of unknown function.[ ... RSV nucleocapsid Naked siRNA Respiratory syncytial virus infections II Completed Alnylam Pharmaceuticals NCT00658086 ... This ancestral RNAi system probably contained at least one dicer-like protein, one argonaute, one PIWI protein, and an RNA- ... Left: A full-length argonaute protein from the archaea species Pyrococcus furiosus. Right: The PIWI domain of an argonaute ...
The S RNA encodes GP and NP (viral nucleocapsid protein) proteins, while L RNA encodes Z and L proteins. The L protein most ... This ensures an adequate supply of viral proteins for subsequent steps of replication, as the NP and L proteins are translated ... The mRNA synthesized from vcRNA are translated to make the GP and Z proteins. This temporal control allows the spike proteins ... for a total of four viral proteins.[15] The large segment encodes a small zinc-binding protein (Z) that regulates transcription ...
Proteins found to be encoded in this virus family genome include reverse transcriptase, proteases, nucleocapsids and ... Virus particles contain nucleocapsids one of two forms; either bacilliform or isometric. The type of nucleocapsid incorporated ... Bacilliform nucleocapsid viruses are approximately 35-50 nm diameter and can be 900 nm in length. Isometric nucleocapsid ... It can either be used as a template for viral protein synthesis, or it can undergo reverse transcription by viral encoded ...
SPP also plays a pathophysiological role; it cleaves the structural nucleocapsid protein (also known as core protein) of the ... In molecular biology, the Signal Peptide Peptidase (SPP) is a type of protein that specifically cleaves parts of other proteins ... In mice, a nonamer peptide originating from the SPP protein serves as minor histocompatibility antigen HM13 that plays a role ... Type IV pilin is a protein found on the surface of Pseudomonas aeruginosa, Neisseria gonorrhoeae and other Gram-negative ...
Receptor Activator of Nuclear Factor κ B (RANK), which is a type I membrane protein that is expressed on the surface of ... January 2002). "Sequence analysis of measles virus nucleocapsid transcripts in patients with Paget's disease". J. Bone Miner. ... which encodes a protein, called p62, that is involved in regulating the function of osteoclasts (bone cells).[5] However, about ...
... envelope proteins (E) Orange deposits: membrane proteins (M) ... അവ S (spike), E (envelope), M (membrane), and N (nucleocapsid) ... Illustration of a SARS-CoV-2 virion Red protrusions: spike proteins (S) Grey coating: the envelope, composed mainly of lipids, ...
... membrane protein; E, envelope protein; N, nucleocapsid protein; structural proteins of coronavirus. ... ရုပ်ဓဇက် ဗီုလဒက်ပတန် ကဝ်ရဝ်နာဝဳရုသ် မထ္ၜးလဝ် အရာမနွံ ပ္ဍဲကဵု ဝဳရုသ်မွဲမ မပ္တံကဵု S, spike protein; M, ... Hamming, I. (2004). "Tissue distribution of ACE2 protein, the functional receptor for SARS coronavirus. A first step in ...
The crystal structure of the Hazara virus nucleocapsid protein. BMC Structural Biology. 2015-01-01, 15: 24. ISSN 1472-6807. PMC ... RadA protein is an archaeal RecA protein homolog that catalyzes DNA strand exchange. Genes Dev. 1998, 12 (9): 1248-53. PMC ... Bank, RCSB Protein Data. RCSB Protein Data Bank - RCSB PDB. (原始内容存档于2012年12月27日).. ... and Evolution of the Crimean-Congo Hemorrhagic Fever Virus Nucleocapsid Protein. Journal of Virology. 2012-10-15, 86 (20): ...
... and the association of viral capsid proteins with viral nucleic acid is called a nucleocapsid. The capsid and entire virus ... The same protein may act as the subunit of both the pentamers and hexamers or they may be composed of different proteins.[81]. ... These proteins go on to become either new virions within the cell, helper proteins, which help assembly of new virions, or ... Transport of viral proteins to the apical membranes and interaction of matrix protein with glycoproteins in the assembly of ...
... capsid and nucleocapsid of the mature virus particles. Like in HIV's replication cycle, these proteins are needed for viral ... When this type of gene is translated to a protein, the protein is called a "transforming protein". Note that since the viral ... Rous sarcoma virus Fusion protein Fusion gene Fusion transcript Chimeric gene Bcr-abl fusion protein Oncovirus Retrovirus ... a Gag-v-Onc fusion protein from the Rous sarcoma virus is useful in illustrating the dual role that the fusion protein plays in ...
Pollard, V. W. and Malim, M. H. (1998). "The HIV-1 Rev protein". Annu. Rev. Microbiol. 52: 491-532. doi:10.1146/annurev.micro. ... Ang isang-hiblang RNA ay masikip na binibigkis sa mga protinang nucleocapsid na p7 at mga ensaym na kailangan sa kaunlaran ng ... 2008). "HIV-1 envelope protein binds to and signals through integrin alpha (4)beta (7), the gut mucosal homing receptor for ... "Endocytosis of major histocompatibility complex class I molecules is induced by the HIV-1 Nef protein". Nat. Med. 2 (3): 338-42 ...
... consists of an outer lipid envelope and an icosahedral nucleocapsid core composed of protein. These virions are 30-42 nm in ... The nucleocapsid encloses the viral DNA and a DNA polymerase that has reverse transcriptase activity.[37] The outer envelope ... Thomas, Bruce (2002). Production of Therapeutic Proteins in Plants. p. 4. ISBN 9781601072542. Archived from the original on 28 ... There are four known genes encoded by the genome, called C, X, P, and S. The core protein is coded for by gene C (HBcAg), and ...
... proteins are targets for antiviral drugs.[43] These proteins are also recognised by antibodies, i.e. they are antigens.[16] The ... Cholesterol-laden membranes with protruding glycoproteins enclose the nucleocapsids; nucleoproteins of different size classes ... accessory proteins and RNA-dependent RNA polymerase into the cytoplasm (Stage 2).[48] These proteins and vRNA form a complex ... The ribonuclear proteins are filamentous and fall in the range of 50 to 130 nm long and 9 to 15 nm in diameter. They have a ...
"NMR structure of the HIV-1 nucleocapsid protein bound to stem-loop SL2 of the psi-RNA packaging signal. Implications for genome ... It has been shown that SL1 may provide a secondary binding site for the viral Rev protein.[15] The Rev protein is an essential ... "Rev protein of human immunodeficiency virus type 1 affects the stability and transport of the viral mRNA". Proceedings of the ... HIV regulatory protein which increases the stability and transport of the unspliced viral RNA.[16] ...
Amacker M, Hottiger M, Mossi R, Hübscher U (1997). „HIV-1 nucleocapsid protein and replication protein A influence the strand ... Replikacioni protein A, 70 kDa DNK vezujuća podjedinica je protein koji je kod ljudi kodiran RPA1 genom.[2] ... Interaction of human rad51 recombination protein with single-stranded DNA binding protein, RPA.". Nucleic Acids Res. 26 (23): ... Replication protein A physically interacts with the Bloom's syndrome protein and stimulates its helicase activity". J. Biol. ...
The RNA genome forms a nucleocapsid along with copies of the 12-kDa capsid protein. The nucleocapsid, in turn, is enveloped ... the RNA genome encodes seven nonstructural proteins and three structural proteins.[24] One of the structural proteins ... Cross-section of Zika virus, showing the viral envelope composed of envelope proteins (red) and membrane proteins (purple) ... This protein is the flavivirus envelope glycoprotein, that binds to the endosomal membrane of the host cell to initiate ...
... capsid and nucleocapsid proteins. In pol, the information for the reverse transcription and integration enzymes are stored. In ... DNA oncoviruses typically impair two families of tumor suppressor proteins: tumor proteins p53 and the retinoblastoma proteins ... the HPV protein E6 binds to a cellular protein called the E6-associated protein (E6-AP, also known as UBE3A), forming a complex ... SV40 uses the same protein for inactivating Rb, LT, to inactivate p53.[39] HPV contains a protein, E7, which can bind to Rb in ...
The most abundant protein produced is the nucleoprotein, whose concentration in the cell determines when L switches from gene ... The virus RdRp partially uncoats the nucleocapsid and transcribes the genes into positive-stranded mRNAs, which are then ... Newly synthesized structural proteins and genomes self-assemble and accumulate near the inside of the cell membrane. Virions ... CryoEM reconstruction of a section of the Marburg virus nucleocapsid. EMDB entry EMD-1986[47] ...
The genome rests within a helical nucleocapsid and is surrounded by matrix protein and an envelope containing viral ... G proteinEdit. Surface protein G (glycoprotein) is primarily responsible for viral attachment to host cells.[23] This protein ... F proteinEdit. Surface protein F (fusion protein) is responsible for fusion of viral & host cell membranes, as well as ... RSV proteins and their function and location in the virion[2][23]. Location in the Virion Protein Alternative Name Function ...
The most abundant protein produced is the nucleoprotein, whose concentration in the cell determines when L switches from gene ... The virus RdRp partially uncoats the nucleocapsid and transcribes the genes into positive-stranded mRNAs, which are then ... Newly synthesized structural proteins and genomes self-assemble and accumulate near the inside of the cell membrane. Virions ... and are not covalently linked to a protein.[22] Marburgvirus genomes are approximately 19 kb long and contain seven genes in ...
For example, in Rhabdoviruses, binding of M proteins to nucleocapsids is accountable for the formation of its "bullet" shaped ... Viral matrix proteins, like many other viral proteins, can exert different functions during the course of the infection. ... Structural proteins linking the viral envelope with the virus core. They play a crucial role in virus assembly, and interact ... An example is the M1 protein of the influenza virus, showing affinity to the glycoproteins inserted in the host cell membrane ...
The DNA is surrounded by a protein nucleocapsid, which is surrounded by a tegument made of protein, which in turn is surrounded ... Protein. Protein. Protein. Protein. Protein. Protein. Protein. Protein. Protein. ncRNAs Latency I. +. -. -. -. -. -. -. -. -. ... Protein/genes[edit]. Protein/gene/antigen Stage Description EBNA-1 latent+lytic EBNA-1 protein binds to a replication origin ( ... These genes also bind the host RBP-Jκ protein. LMP-1 latent LMP-1 is a six-span transmembrane protein that is also essential ...
A large number of nucleocapsids are produced in the neurons and the glial cells. In these cells the viral genes that encode ... envelope proteins have restricted expression.[4] As a result, infectious particles like the M protein are not produced, and the ...
HIV-1 nucleocapsid protein (NC) is a small basic protein generated by the cleavage of the Gag structural polyprotein precusor ... fraying and annealing properties of the HIV nucleocapsid protein. Top, from left to right: NC protein is represented by an oval ... Properties and functions of the nucleocapsid protein in virus assembly.. Muriaux D1, Darlix JL. ... HIV-1 NC and more generally retroviral NC proteins are nucleic acid binding proteins possessing potent nucleic acid condensing ...
Characterization of expression of Puumala virus nucleocapsid protein in transgenic plants.. Khattak S1, Darai G, Süle S, Rösen- ... In the present study, the expression of the nucleocapsid (N) protein of hantavirus serotype Puumala in tobacco and potato ... The antigens were expressed at a level of 1 ng of protein/5 microg of dried leaves. The hantaviral recombinant N proteins ... In order to investigate the distribution of the expression of the foreign gene in transgenic plants, the proteins of leaves and ...
Retroviral nucleocapsid proteins (NCPs) are CCHC-type zinc finger proteins that mediate virion RNA binding activities ... Retroviral nucleocapsid proteins (NCPs) are CCHC-type zinc finger proteins that mediate virion RNA binding activities ... nucleocapsid protein.. Gao, Y., Kaluarachchi, K., Giedroc, D.P.. (1998) Protein Sci 7: 2265-2280 ... NUCLEOCAPSID PROTEIN FROM MASON-PFIZER MONKEY VIRUS (MPMV). *DOI: 10.2210/pdb1CL4/pdb ...
Despite such importance, no proteins of tospoviruses have been elucidated so far. Among TSWV-encoded proteins, the N protein is ... Asymmetric Trimeric Ring Structure of the Nucleocapsid Protein of Tospovirus.. Komoda, K., Narita, M., Yamashita, K., Tanaka, I ... Similar to those of all other negative-strand RNA viruses, the nucleocapsid (N) protein plays very important roles in its viral ... Unlike N proteins of other negative-strand RNA viruses, the TSWV N protein forms an asymmetric trimeric ring. To form the ...
Opportunity For Licensing: HIV-1 Nucleocapsid Protein (p7nc) Capture Assay. A Notice by the Health and Human Services ...
Browse our SARS Nucleocapsid Protein Antibody catalog backed by our Guarantee+. ... SARS Nucleocapsid Protein Antibodies available through Novus Biologicals. ... anti-Nucleocapsid protein antibody, anti-SARS coronavirus N protein antibody, anti-SARS coronavirus nucleocapsid protein ... SARS Nucleocapsid Protein Antibodies. We offer SARS Nucleocapsid Protein Antibodies for use in common research applications: ...
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Rabbit polyclonal H1N1 Influenza A virus Nucleocapsid protein antibody validated for WB, ELISA, ICC/IF and tested in InfA. ... Anti-H1N1 Influenza A virus Nucleocapsid protein antibody. See all H1N1 Influenza A virus Nucleocapsid protein primary ... Synthetic peptide derived from internal part of H1N1 Influenza A virus Nucleocapsid protein. ... Proteins and Peptides. Proteomics tools. Agonists, activators, antagonists and inhibitors. Lysates. Multiplex miRNA assays. By ...
Rabbit polyclonal H1N1 Influenza A virus Nucleocapsid protein antibody validated for ICC/IF and tested in InfA. Immunogen ... Anti-H1N1 Influenza A virus Nucleocapsid protein antibody. See all H1N1 Influenza A virus Nucleocapsid protein primary ... Proteins and Peptides. Proteomics tools. Agonists, activators, antagonists and inhibitors. Lysates. Multiplex miRNA assays. By ... Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. ...
Recently, we have shown that wild-type MeVs induce the suppression of protein synthesis in host cells (referred to as ... Furthermore, recombinant MeV lacking the La-binding motif in the N-5UTR displayed delayed viral protein synthesis and growth ... of nucleocapsid (N) mRNA. The La/SSB autoantigen (La) was found to specifically bind to an N-5UTR probe. Recombinant La ... Recently, we have shown that wild-type MeVs induce the suppression of protein synthesis in host cells (referred to as shutoff ...
Computer model showing the hexameric structure of the toscana virus nucleocapsid protein. - Stock Image C035/6329 ... Toscana virus nucleocapsid protein. Computer model showing the hexameric structure of the toscana virus nucleocapsid protein. ... nucleocapsid, protein, space-fill, space-filled, spacefill, spacefilled, structure, toscana, virus ...
Molecular model of the nucleocapsid protein (blue) from HIV-1 (human immunodeficiency virus-type 1) complexed with the Psi RNA ... Caption: HIV nucleocapsid protein. Molecular model of the nucleocapsid protein (blue) from HIV-1 (human immunodeficiency virus- ... nucleocapsid, nucleocapsid protein, plain background, protein, psi element, psi rna, retroviral, retrovirus, ribonucleic acid, ...
Recombinant Truncated Nucleocapsid Protein as Antigen in a Novel Immunoglobulin M Capture Enzyme-Linked Immunosorbent Assay for ... Comparison of Immunoglobulin G Responses to the Spike and Nucleocapsid Proteins of Severe Acute Respiratory Syndrome (SARS) ... Characterization of Monoclonal Antibodies to Junin Virus Nucleocapsid Protein and Application to the Diagnosis of Hemorrhagic ... Use of Saccharomyces cerevisiae-Expressed Recombinant Nucleocapsid Protein To Detect Hantaan Virus-Specific Immunoglobulin G ( ...
The nucleocapsid (N) gene of turkey coronavirus (TCV) was amplified by reverse transcriptase-polymerase chain reaction, cloned ... one of these proteins, p52, was comparable in size to native TCV N protein. Baculovirus-expressed N proteins were used as ... Baculovirus expression of turkey coronavirus nucleocapsid protein Avian Dis. Jan-Mar 2001;45(1):136-43. ... The nucleocapsid (N) gene of turkey coronavirus (TCV) was amplified by reverse transcriptase-polymerase chain reaction, cloned ...
We observed an increase in caspase-7 and -8, but not -9 in cells expressing HTNV N protein mutants lacking amino acids 270-330 ... nucleocapsid (N) protein and the modulation of apoptosis. ... nucleocapsid (N) protein and the modulation of apoptosis. We ... Modulation of apoptosis and immune signaling pathways by the Hantaan virus nucleocapsid protein Virology. 2010 Jun 5;401(2):165 ... Further, TNFR stimulated cells expressing HTNV N protein inhibited caspase activation. In contrast, cells expressing N protein ...
Among these proteins, the HIV-1 nucleocapsid protein (NC) is a highly conserved protein in diverse HIV subtypes that plays a ... Final Report Summary - THINPAD (Targeting the HIV-1 Nucleocapsid Protein to fight Antiretroviral Drug Resistance). Executive ... The optimization process derived from the in silico studies has led to the discovery of new inhibitors of the HIV-NC protein ... The optimization process in the pyrimidine series has led to the discovery of new inhibitors of the HIV-NC protein that show ...
In this study, we focus on the SARS-CoV-2 nucleocapsid protein as a suitable candidate target for vaccine formulations. Major B ... Most notably there is a region of potential T cell cross-reactivity within the SARS-CoV-2 N protein position 102-110 amino ... Major B and T cell epitopes of the SARS-CoV-2 N protein are predicted and resulting sequences compared with the homologue ... Most notably there is a region of potential T cell cross reactivity within the SARS-CoV-2 N protein position 102-110 amino ...
Locate proteins, assay kits, reagents, custom services. Get complete supplier details here. ... Need SARS-CoV-2 Nucleocapsid protein for research? Find and compare commercial and governmental sources for immunological and ... SARS-CoV-2 Nucleocapsid protein. Description. Recombinant SARS-CoV-2 Nucleocapsid protein. MW: 46 kDa. Purity >90% by SDS-PAGE ... SARS-CoV-2 Nucleocapsid protein from BIORBYT LTD.. Search, find, compare suppliers for SARS-CoV-2 Nucleocapsid protein products ...
... indicating that the nucleocapsid shell (protein P8) has undergone partial disassembly exposing the proteins antigenic sites. ... The location of P7 in the post-packaged nucleocapsid (NC) remains unknown. P7 may translocate closer to the five-fold axis of a ... In the unpackaged procapsid, the protein is situated in close proximity to the viral directed RNA polymerase, P2. Cryo-electron ... It is further demonstrated that within ϕ6 nucleocapsids that are primed for early-phase transcription, P7 is partially ...
Protein - Cat. No. 10-067 , ProSci can be used to detect Virus Recombinant SARS-CoV-2, Nucleocapsid (N) Protein - 100 µg in ... Purified SARS-CoV-2 Nucleocapsid (N) Protein. Lane 1 - Nucleocaspid protein under reducing conditions loaded at 10 μg. Lane 2 ... and nucleocapsid protein (N) 2. The S protein is a transmembrane, homotrimeric, class I fusion glycoprotein that mediates viral ... The predicted molecular weight of Recombinant SARS-CoV-2, Nucleocapsid (N) Protein is Mr 47 kDa. ...
The Nucleocapsid Protein of SARS-CoV-2: a Target for Vaccine Development. Noton K. Dutta, Kaushiki Mazumdar, James T. Gordy ... The SARS coronavirus nucleocapsid protein-forms and functions. Antiviral Res 103:39-50. doi:10.1016/j.antiviral.2013.12.009. ... Nucleocapsid protein recruitment to replication-transcription complexes plays a crucial role in coronaviral life cycle. J Virol ... New reports have additionally shown that the crystal structure of the SARS-CoV-2 nucleocapsid protein is similar to those of ...
... and nucleocapsid (N) proteins, are also well annotated (7). According to previous reports, the M and E proteins are necessary ... nucleocapsid protein (rN) and spike protein (rS) were used for detecting IgM and IgG antibodies, and their diagnostic ... The nucleocapsid protein of coronaviruses acts as a viral suppressor of RNA silencing in mammalian cells. J Virol 89:9029-9043. ... Identification of in vivo-interacting domains of the murine coronavirus nucleocapsid protein. J Virol 83:7221-7234. doi:10.1128 ...
... are small highly basic proteins, characterized by the presence of two CCHC ZF (zinc finger) domains. NCps, closely associated ... but the essential residues responsible for the interaction with oligonucleotides adopt the same orientation in the two proteins ... nucleocapsid proteins) of HIV-1 (HIV type 1), HIV-2 and SIV (simian immunodeficiency virus) ... nucleocapsid proteins) of HIV-1 (HIV type 1), HIV-2 and SIV (simian immunodeficiency virus) are small highly basic proteins, ...
... from NURSING 170114110 at Arab American University ... capsid proteins = Nucleocapsids  Some viruses have envelope surround the capsid composed of lipid, carbohydrates and proteins ... Viral nucleocapsids have two forms of symmetry:  Polyhedral : in which the capsomers are arranged in 20 triangles that form a ... All human viruses that have a helical nucleocapsid are enclosed by envelope(outer membrane).  There are no naked helical ...
Rescue of Recombinant Marburg Virus from cDNA Is Dependent on Nucleocapsid Protein VP30. Sven Enterlein, Viktor Volchkov, ... Rescue of Recombinant Marburg Virus from cDNA Is Dependent on Nucleocapsid Protein VP30 ... Rescue of Recombinant Marburg Virus from cDNA Is Dependent on Nucleocapsid Protein VP30 ... Rescue of Recombinant Marburg Virus from cDNA Is Dependent on Nucleocapsid Protein VP30 ...
Sequence analysis of the porcine transmissible gastroenteritis coronavirus nucleocapsid protein gene. KAPKE P. A. ... Comparison of the Amino Acid Sequence and Phylogenetic Analysis of the Peplomer, Integral Membrane and Nucleocapsid Proteins of ... Primary structure of the membrane and nucleocapsid protein genes of feline infectious peritonitis virus and immunogenicity of ... cDNA cloning and sequence analysis of the gene encoding the peplomer protein of feline infectious peritonitis virus. GROOT R. J ...
Recombinant protein - Proteins. SARS - Recombinant protein - Proteins. Recombinant protein - for Western blotting - Proteins. ... Recombinant protein - tag: Tagless - Proteins. Recombinant protein - from E. coli - Proteins. SARS - Proteins. SARS - for ... SARS-CoV - Proteins. SARS-CoV - Recombinant protein - Proteins. SARS - SARS-CoV - Proteins. SARS-CoV - for Western blotting - ... You are here: Home Products and Services Proteins SARS-CoV Nucleocapsid Protein E. coli ...
Antigenicity of severe fever with thrombocytopenia syndrome virus nucleocapsid protein and its potential application in the ... Antigenicity of severe fever with thrombocytopenia syndrome virus nucleocapsid protein and its potential application in the ...
Nucleocapsid Protein ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate. The plate is ... Anti-N, Anti-Nucleocapsid, SARS-CoV-2 Antigen, Nucleocapsid phosphoprotein, Nucleoprotein, SARS-CoV Nucleocapsid Protein Ave. ... BioLegends LEGEND MAX™ SARS-CoV2- Nucleocapsid Protein ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with ... This kit is specifically designed for the accurate quantitation of SARS-CoV-2 Nucleocapsid Protein from cell culture ...
... binding domain in the nucleocapsid (N) protein of hantavirus, the RNA virus responsible for causing haemorrhagic fever and ... The authors performed structural and functional analysis to identify and characterize the ribosomal protein S19 (RPS-19) ... Ribosomal Protein S19 Binding Domain Provides Insights into Hantavirus Nucleocapsid Protein-mediated Translation Initiation ... binding domain in the nucleocapsid (N) protein of hantavirus, the RNA virus responsible for causing haemorrhagic fever and ...
  • Each SARS Nucleocapsid Protein Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
  • Choose from our SARS Nucleocapsid Protein polyclonal antibodies and browse our SARS Nucleocapsid Protein monoclonal antibody catalog. (novusbio.com)
  • Most vaccine studies so far have focused on antibody responses generated against the S protein, the most exposed protein of SARS-CoV-2 ( 10 , 11 ). (frontiersin.org)
  • Shin GC, Chung YS, Kim IS, Cho HW, Kang C. Preparation and characterization ofa novel monoclonal antibody specific to severe acute respiratorysyndrome-coronavirus nucleocapsid protein. (biovendor.com)
  • HSV-1 Nucleocapsid Protein antibody LS-C546713 is an HRP-conjugated mouse monoclonal antibody to herpes simplex virus 1 HSV-1 Nucleocapsid Protein. (lsbio.com)
  • Search, find, compare suppliers for anti-Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2/COVID-19) Nucleocapsid Protein antibody, protein, ELISA kits. (linscottsdirectory.com)
  • SARS-CoV-2/COVID-19 Nucleocapsid Protein Antibody (FITC). (linscottsdirectory.com)
  • Site-directed mutagenesis, CD spectroscopy, and immunocytochemistry reveal that a point mutation in the conserved basic surface formed by Arg22 or Lys26 lead to antibody recognition based on the subcellular localization of the N protein. (ku.edu)
  • In order to determine if the nucleocapsid protein of MERS-CoV (MERS-N) plays a role in viral-host interactions, a murine monoclonal antibody was generated so as to allow detection of the protein in infected cells as well as in overexpression system. (bioscirep.org)
  • In the infected cell, N was the only viral protein that was phosphorylated as shown by 32P-orthophosphate labeling and Western blot analysis and with IBV specific polyclonal chicken antibody. (tamu.edu)
  • One-hour pulse-chase analysis followed by immunoprecipitation of IBV N using rabbit anti-IBV N polyclonal antibody showed that the phosphate on the protein did not fall below 70% of the maximum and remained stable. (tamu.edu)
  • The highly conserved IBV N protein was also used as a diagnostic tool in an ELISA for detecting anti-IBV antibody in chicken serum using a specialized microwave called the BIOWAVE. (tamu.edu)
  • All Nucleocapsid/NP reagents are produced in house and quality controlled, including 12 Nucleocapsid/NP Antibody, 75 Nucleocapsid/NP Gene, 7 Nucleocapsid/NP IP Kit, 9 Nucleocapsid/NP Lysate, 11 Nucleocapsid/NP Protein. (sinobiological.com)
  • No. NUN-C52Hr ) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG1 (Cat. (acrobiosystems.com)
  • The purity of Anti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG1 (Cat. (acrobiosystems.com)
  • Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2/COVID-19) Nucleocapsid Protein (NP) Antibody is a Mouse Monoclonal Antibody against SARS-CoV-2/COVID-19 NP. (abbexa.com)
  • Purified mouse monoclonal antibody (IgG2b) raised against recombinant Nucleocapsid Protein (NP) from SARS-CoV-2 (COVID-19). (bioservuk.com)
  • Immobilized SARS-CoV-2 (2019-nCoV) NP Antibody (6G9)(Cat#DA027) at 5μg/ml (100 μl/well) showing the binding to SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein-His (Cat#DRA31). (bonopusbio.com)
  • Persistence and decay of human antibody responses to the receptor binding domain of SARS-CoV-2 spike protein in COVID-19 patients. (cdc.gov)
  • In a subset of 15 individuals with samples collected up to 75 days post-symptom onset, neutralizing antibody responses against the spike protein were measured. (cdc.gov)
  • Over 280,000 products but you can't find the right antibody for your protein or application? (acris-antibodies.com)
  • Our SARS Nucleocapsid Protein Antibodies can be used in a variety of model species: Human, Virus. (novusbio.com)
  • Baculovirus-expressed N proteins were used as antigen in an indirect enzyme-linked immunosorbent assay (ELISA) for detection of TCV-specific antibodies. (nih.gov)
  • These findings indicate that baculovirus-expressed TCV N protein is a suitable source of antigen for ELISA-based detection of TCV-specific antibodies in turkeys. (nih.gov)
  • High levels of IgG antibodies against N have been detected in sera from SARS patients ( 18 ), and the N protein is a representative antigen for the T-cell response in a vaccine setting, inducing SARS-specific T-cell proliferation and cytotoxic activity ( 19 , 20 ). (asm.org)
  • We have already shown that the middle or C-terminal region of the SARS-CoV N protein is important for eliciting antibodies against SARS-CoV during the immune response ( 21 - 23 ). (asm.org)
  • Two enzyme-linked immunosorbent assay (ELISA) kits based on recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid protein (rN) and spike protein (rS) were used for detecting IgM and IgG antibodies, and their diagnostic feasibility was evaluated. (asm.org)
  • non-neutralizing antibodies against N protein were found to protect mice against lethal infection. (biovendor.com)
  • Nine monoclonal antibodies (MAB) against nucleocapsid protein (NP) of Newcastle disease virus (NDV) have been prepared and characterized. (eurekamag.com)
  • SARS coronavirus nucleocapsid protein monoclonal antibodies de. (mysciencework.com)
  • SARS coronavirus nucleocapsid protein monoclonal antibodies developed using a prokaryotic expressed protein. (mysciencework.com)
  • An indirect enzyme-linked immunosorbent assay (ELISA) based on the recombinant nucleocapsid (rN) protein which has been developed for antibodies detection against Winter Dysentery Disease was produced by an Escherichia coli protein expression system. (scitechnol.com)
  • IgA and IgM antibodies to the SARS-CoV-2 receptor-binding domain (RBD) of the spike protein were short-lived. (cdc.gov)
  • Study used enzyme-linked immunosorbent assays (ELISAs) to monitor levels of IgG, IgA and IgM antibodies to the SARS-CoV-2 spike protein and RBD in 343 patients with RT-PCR-confirmed SARS-CoV-2 infection (with 93% requiring hospitalization), up to 122 days post-symptom onset. (cdc.gov)
  • IgG antibodies to both the SARS-CoV-2 spike protein and its receptor-binding domain (RBD) peaked in serum by 16-30 days post-symptom onset and were sustained through 105-115 days (Figure). (cdc.gov)
  • Study used enzyme-linked immunosorbent assays (ELISAs) to monitor IgG, IgA and IgM antibodies levels to the SARS-CoV-2 spike protein and RBD in acute and convalescent serum from 439 persons and saliva from 128 persons, 3-115 days post-symptom onset. (cdc.gov)
  • Antigenic and structural variation in the major nucleocapsid protein, VPN41, from different strains of respiratory syncytial (RS) virus was observed using a combination of monoclonal antibodies and two-dimensional peptide mapping. (microbiologyresearch.org)
  • Two monoclonal antibodies, N1 and N2, reactive with primary sequence epitopes located on intact nucleocapsids also reacted with either the 27K fragment (N2) or the 14K fragment (N1). (microbiologyresearch.org)
  • Recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein is produced by our E.coli expression system and the target gene encoding Met1-Ala419 is expressed with a 6His tag at the N-terminus. (bonopusbio.com)
  • The ED50 of SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein-His (Cat#DRA31) is 68 ng/ml. (bonopusbio.com)
  • A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (YP_009724397.2(335Gly/Ala)) (Met1-Ala419) was expressed with a polyhistidine tag at the C-terminus. (sinobiological.com)
  • The recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (His tag) consists of 430 amino acids and predicts a molecular mass of 47.08 kDa. (sinobiological.com)
  • Major B and T cell epitopes of the SARS-CoV-2 N protein are predicted and resulting sequences compared with the homolog immunological domains of other coronaviruses that infect human beings. (frontiersin.org)
  • N proteins of many coronaviruses are highly immunogenic and are expressed abundantly during infection ( 17 ). (asm.org)
  • Like other known coronaviruses, SARS-CoV is an enveloped virus containing three outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. (biovendor.com)
  • Even though nidovirus replication is restricted to the cytoplasm, the nucleocapsid protein (N) of several coronaviruses and arteriviruses, localize to the nucleolus during infection. (k-state.edu)
  • The sequences contained a 1323 nucleotide (nt) open reading frame (ORF), which showed moderate identity to the nucleocapsid (N) gene of other coronaviruses. (eurekamag.com)
  • The N protein of MERS-CoV (to be referred to as MERS-N herein) is a 413 amino acids protein and has been reported to share some characteristics with N of other coronaviruses. (bioscirep.org)
  • The structural proteins include spike (S), envelope (E), membrane (M) and nucleocapsid (N) proteins.Nucleocapsid protein is one of the most crucial structural components of coronaviruses;hence major attention has been focused on characterization of this protein. (uwc.ac.za)
  • Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. (sinobiological.com)
  • Nucleocapsid protein (NP) is the most abundant protein on the helical nucleocapsid of coronaviruses, which envelopes the entire genomic RNA. (immunodiagnostics.com.hk)
  • MERS-CoV, which causes the Middles East Respiratory Syndrome (MERS), belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). (bio-techne.com)
  • Toroviruses and a select subset of coronaviruses (in particular the members of subgroup A in the genus Betacoronavirus) possess, in addition to the peplomers composed of S, a second type of surface projections composed of the hemagglutinin-esterase protein. (wikipedia.org)
  • MeV has six genes on its genome [nucleocapsid (N), phospho (P), matrix (M), fusion (F), hemagglutinin (H), and large (L)] and produces eight proteins (V and C proteins are produced from the P gene). (frontiersin.org)
  • The coronaviral genome encodes a relatively small number of proteins, classified as either structural or non-structural. (frontiersin.org)
  • The SARS-CoV-2 genome, which shares 79.6% identity with SARS-CoV, encodes four essential structural proteins: the spike (S), envelope (E), membrane (M), and nucleocapsid protein (N) 2. (prosci-inc.com)
  • SARS-CoV-2 has a single-stranded, plus-sense, RNA genome of approximately 30 kb, which includes five major open reading frames encoding nonstructural replicase polyproteins and structural proteins ( 1 ), namely, spike (S) ( 4 - 6 ), envelope (E), membrane (M), and nucleocapsid (N) ( 7 ), and they are in the same order and of approximately the same sizes as those in SARS-CoV. (asm.org)
  • 17 ) used a mouse hepatitis virus (MHV) model to show that the viral nucleocapsid (N) protein contributes to forming helical ribonucleoproteins during the packaging of the RNA genome, regulating viral RNA synthesis during replication and transcription and modulating metabolism in infected subjects. (asm.org)
  • The nucleocapsid (N) protein together with the viral RNA genome form a helical core located within the viral envelope. (biovendor.com)
  • The highly basic 55 amino acid nucleocapsid protein (NC) that coats the HIV-1 genome has two zinc fingers that differ by five amino acids (strain pNL4-3). (umd.edu)
  • The nucleocapsid (N) is the most abundant viral protein, and during viral assembly, the N protein forms trimers and packages the viral RNA genome. (ku.edu)
  • The nucleocapsid (N) protein of the pneumovirus respiratory syncytial virus (RSV) is a major structural protein which encapsidates the RNA genome and is essential for replication and transcription of the RSV genome. (microbiologyresearch.org)
  • MERS-CoV is an enveloped, single-stranded, positive-sense RNA virus, approximately 30 kb in length with two-thirds of its genome encoding 15-16 non-structural proteins (nsp) [ 6 , 7 ]. (bioscirep.org)
  • Influenza A viral nucleoprotein (NP) plays a critical role in virus replication and host adaptation.The influenza A virus nucleoprotein (NP) is an essential multifunctional protein that encapsidates the viral genome and functions as an adapter between the virus and the host cell machinery. (sinobiological.com)
  • The 5′-two thirds of the genome encodes for a polyprotein that contains all the enzymes necessary for replication, whereas the 3′-one third encodes for all the structural proteins that mediate viral entry into the host cell. (uwc.ac.za)
  • Importantly, we demonstrated that the interaction between VP24 and NP was required for both nucleocapsid assembly and genome packaging. (nature.com)
  • Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. (sinobiological.com)
  • CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. (acrobiosystems.com)
  • The nucleocapsid protein (NP) packages the positive strand viral genome RNA of the virus into a helical ribonucleocapsid (RNP) and helps with virion assembly. (cellecta.com)
  • It has a tripartite genome consisting of negative sense RNA segments called large (L), medium (M) and small (S). The S segment encodes the nucleocapsid protein (N) of 233 amino acids. (st-andrews.ac.uk)
  • The nucleocapsid (N) protein is the structural component of the viral structure and it primarily functions to bind coronavirus RNA genome. (exonbio.com)
  • The human immunodeficiency virus type-1 (HIV-1) nucleocapsid protein (NC) is an essential and multifunctional protein involved in multiple stages of the viral life cycle such as reverse transcription, integration of proviral DNA, and especially genome RNA packaging. (biomedcentral.com)
  • While the S, E and M proteins build up the viral envelope, the N protein is involved transcription, replication and packaging of the viral RNA genome into a helical ribonucleocapsid (RNP) (1, 2). (bio-techne.com)
  • N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. (bonopusbio.com)
  • nonglycosolated virion envelope protein (24).The complete nucleotide sequence for the RNA genome of yellow fever virus has been determined (24). (cdc.gov)
  • A unique feature of the genome is the M2 gene, which encodes proteins M2-1 and M2-2. (wikipedia.org)
  • Once sufficient P, N, L, and M2 proteins are available to create a capsid around the newly replicated genome, the virus undergoes replication. (wikipedia.org)
  • The SARS-CoV-2 Nucleoprotein (also known as Nucleocapsid Protein or N Protein) is a protein that binds to the RNA in the viral particle. (linscottsdirectory.com)
  • The nucleoprotein is the second most-common protein in SARS-CoV-2 targeted by the immune system, after the spike protein. (linscottsdirectory.com)
  • In some cases it depends on the extent of nucleoprotein modification (phosphorylation, etc), however also different degree of oligomerization is often important for higher reactivity of these recombinant proteins. (baltymas.lt)
  • A DNA sequence encoding the Influenza A virus (A/Shanghai/2/2013(H7N9)) nucleoprotein protein (AGL44439.1) (Met1-Asn498) was expressed with a C-terminal polyhistidine tag. (sinobiological.com)
  • The recombinant nucleoprotein protein of Influenza A virus (A/Shanghai/1/2013(H7N9)) comprises 509 amino acids and has a predicted molecular mass of 57.8 kDa. (sinobiological.com)
  • HIV-1 nucleocapsid protein (NC) is a small basic protein generated by the cleavage of the Gag structural polyprotein precusor by the viral protease during virus assembly in the infected cell. (nih.gov)
  • As such NC protein drives critical structural rearrangements of the genomic RNA, notably RNA dimerization in the course of virus assembly and viral nucleic acid annealing required for genomic RNA replication by the viral reverse transcriptase (RT). (nih.gov)
  • Among structural proteins, the spike glycoprotein (S), and the nucleocapsid protein (N) are the major ones, while the envelope protein (E) and membrane protein (M) are smaller structural components ( 3 , 4 ). (frontiersin.org)
  • This first reported structure of a simian NCp8 compared with that of NCp7 shows that the main structural differences occur at the flexible linker between the two ZFs but the essential residues responsible for the interaction with oligonucleotides adopt the same orientation in the two proteins. (archives-ouvertes.fr)
  • As the most abundantly expressed structural protein during infection, SARS-CoV NP is highly detectable in SARS patients. (biovendor.com)
  • The authors performed structural and functional analysis to identify and characterize the ribosomal protein S19 (RPS-19) binding domain in the nucleocapsid (N) protein of hantavirus, the RNA virus responsible for causing haemorrhagic fever and hantavirus cardiopulmonary syndrome. (moleculardevices.com)
  • Functional and structural characterization of 2-amino-4-phenylthiazole inhibitors of the HIV-1 nucleocapsid protein with antiviral activity. (semanticscholar.org)
  • HIV-1 nucleocapsid protein as a nucleic acid chaperone: spectroscopic study of its helix-destabilizing properties, structural binding specificity, and annealing activity. (semanticscholar.org)
  • Sequence of the major nucleocapsid protein gene of pneumonia virus of mice: sequence comparisons suggest structural homology between nucleocapsid proteins of pneumoviruses, paramyxoviruses, rhabdoviruses and filoviruses. (microbiologyresearch.org)
  • The remaining one-third encodes for structural proteins interspersed with accessory proteins. (bioscirep.org)
  • The structural proteins include the spike (S), matrix (M), envelope (E), and nucleocapsid (N) proteins [ 6 , 7 ]. (bioscirep.org)
  • The coronavirus nucleocapsid (N) is a structural protein that forms complexes with genomic RNA, interacts with the viral membrane protein during virion assembly, and plays a critical role in enhancing the efficiency of virus transcription and assembly. (sinobiological.com)
  • The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. (acrobiosystems.com)
  • NP also interacts with other viron structural proteins to play important roles during host cell entry and virus particle assembly and release. (immunodiagnostics.com.hk)
  • The MERS-CoV N protein is a ~45 kDa protein composed of two independent structural domains connected by a linker region. (bio-techne.com)
  • Another important structural protein is the phosphoprotein N, which is responsible for the helical symmetry of the nucleocapsid that encloses the genomic RNA. (wikipedia.org)
  • The L gene encodes the L protein, an RNA-dependent RNA polymerase that transcribes the template RNA into new genomic RNA and viral mRNAs, together with the N and P proteins ( Griffin, 2007 ). (frontiersin.org)
  • It is about 15 kbp in size, and encodes eleven proteins. (wikipedia.org)
  • All human viruses that have a helical nucleocapsid are enclosed by envelope(outer membrane). (coursehero.com)
  • The type III secretion apparatus (TTSA) functions like a syringe to inject proteins through an external needle and into a target cell's membrane and cytosol. (ku.edu)
  • Targeted RNA recombination of the membrane and nucleocapsid protein genes between mouse hepatitis virus and bovine coronavirus. (illinois.edu)
  • Fingerprint Dive into the research topics of 'Targeted RNA recombination of the membrane and nucleocapsid protein genes between mouse hepatitis virus and bovine coronavirus. (illinois.edu)
  • Then, through the action of the fusion protein, the virus fuses to the host plasma membrane and the nucleocapsid is released. (wikipedia.org)
  • Retroviral nucleocapsid proteins (NCPs) are CCHC-type zinc finger proteins that mediate virion RNA binding activities associated with retrovirus assembly and genomic RNA encapsidation. (rcsb.org)
  • Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. (abcam.com)
  • Briedis, D. J. 1997-02-01 00:00:00 The currently accepted model for measles virus (MV) transcription and replication assumes the nucleocapsid (NP) protein to possess the ability to bind to RNA, to other NP molecules, and to the phosphoprotein (P) during ribonucleocapsid (RNP) assembly, as well as to the matrix protein (M) during virion assembly. (deepdyve.com)
  • Using pulse-labeling with 32Porthophosphate, the IBV N protein was found to be phosphorylated in the virion, as well as at all times during infection of Vero cells. (tamu.edu)
  • Simultaneous labeling with 32Porthophosphate and 3H-leucine of infected CEK cells indicated a 3.5-fold increase in the ratio of the 32P:3H counts per minute (cpm) on the virion N protein as compared to the 32P:3H cpm ratio of the N protein from lysates at 7 h p.i. (tamu.edu)
  • The 32P:3H cpm ratio of the N protein from virion from infected-Vero cell lysates was 10.5X more than the 32P:3H cpm ratio of the N protein obtained at 7 h p.i. (tamu.edu)
  • Indeed, we directly demonstrate here that the interaction between NP and VP24 is essential for nucleocapsid formation and packaging into the virion. (nature.com)
  • During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. (sinobiological.com)
  • In contrast, N protein homodimers isolated from extracellular virions were shown to have formed NEM-resistant intermolecular disulfide linkages, the function of which is probably to impart stability to the virion. (illinois.edu)
  • During virion assembly, it packages the viral RNA into a helical nucleocapsid and is highly associated with transcription and replication of the virus. (bonopusbio.com)
  • The SARS-CoV-2 S protein is being used as the leading target antigen in vaccine development ( 8 , 9 ). (asm.org)
  • The epitope antigen N protein was designed using almost total N gene fragments (8-430 aa, N gene). (scitechnol.com)
  • The rN protein was standardized with a coating antigen concentration of 5 μg/well. (scitechnol.com)
  • The spike (S) protein is arrayed on the surface of the virus particles, giving the characteristic 'crown' appearance ( 5 ). (frontiersin.org)
  • Additionally, the spike protein has several hotspots for mutations ( 15 ), whereas the nucleocapsid gene is more stable and has acquired fewer mutations to date ( 16 ). (frontiersin.org)
  • A) The location of the NTD protein on the Middle East respiratory syndrome coronavirus MERS-CoV spike (S) protein. (bioz.com)
  • Spike Mutant Proteins-UK, South African, and Brazilian Variants. (rndsystems.com)
  • Our SARS-CoV-2 Spike proteins exhibit high affinity binding to human ACE-2 in both ELISA and SPR. (rndsystems.com)
  • Spike proteins from the U.K., South African, and Brazilian variants are now available. (rndsystems.com)
  • View our available Spike protein mutants and those in development in the table below. (rndsystems.com)
  • IgG and IgM levels against the spike protein and RBD were correlated across 71 paired serum and saliva samples. (cdc.gov)
  • In order to investigate the distribution of the expression of the foreign gene in transgenic plants, the proteins of leaves and roots of the individual transgenic tobacco and potato plants were examined by Western blot analyses. (nih.gov)
  • The nucleocapsid (N) gene of turkey coronavirus (TCV) was amplified by reverse transcriptase-polymerase chain reaction, cloned, and expressed in the baculovirus expression system. (nih.gov)
  • A recombinant baculovirus containing the TCV N gene (rBTCV/N) was identified by polymerase chain reaction and expression of TCV N protein as determined by western immunoblot analysis. (nih.gov)
  • The SARS-CoV-2 S gene has 76% amino acid similarity to the SARS-CoV S gene ( 11 ), and nonsynonymous mutations developed in the S protein as the SARS-CoV epidemic progressed ( 12 , 13 ). (asm.org)
  • The nucleotide sequence of the peplomer gene of porcine transmissible gastroenteritis virus(TGEV):comparison with the sequence of the peplomer protein of feline infectious peritonitis virus(FIPV). (nii.ac.jp)
  • Nucleotide sequence of the porcine transmissible gastroenteritis coronavirus matrix protein gene. (nii.ac.jp)
  • Confocal microscopy of N protein localization in Vero cells infected with the SARS-CoV or transfected with the SARS-CoV N gene failed to show presence of N in the nucleoplasm or nucleolus. (k-state.edu)
  • A second 336 nt ORF, which might encode a leucine-rich protein similar to, but shorter than, the bovine coronavirus internal protein was found within the PEDV N gene. (eurekamag.com)
  • To express and purify the nucleocapsid protein (N protein) of SARS-CoV and generate MAbs against the N protein, gene encoding N protein was separated into two parts according to the prediction of epitopes and cloned into pET32a(+), respectively. (mysciencework.com)
  • In the present study, the N195-Sc fusion protein was highly expressed in insect (Sf9) cells infected with a recombinant baculovirus bearing the hybrid gene under the control of a polyhedrin promoter. (nus.edu.sg)
  • All SFTSV Nucleocapsid reagents are produced in house and quality controlled, including 15 SFTSV Nucleocapsid Gene. (348702.live)
  • Protein labelling and western blotting showed that mutations in the N gene did not affect expression of the other viral genes as much as affecting N protein expression. (st-andrews.ac.uk)
  • Synthetic peptide derived from internal part of H1N1 Influenza A virus Nucleocapsid protein. (abcam.com)
  • Synthetic peptide from the H1N1 Influenza A virus Nucleocapsid protein (ACQ76305). (abcam.com)
  • Analysis of NCp7-dependent activation of HIV-1 cDNA integration and its conservation among retroviral nucleocapsid proteins. (semanticscholar.org)
  • SFTSV Nucleocapsid cDNA clones are full length sequence confirmed and expression validated. (348702.live)
  • To investigate residues in BUNV N crucial for its functionality, random and site- specific mutagenesis were performed on a cDNA clone encoding the BUNV N protein. (st-andrews.ac.uk)
  • Facilitates nucleocapsid and envelope interactions. (wikipedia.org)
  • The viral envelope, obtained by budding through membranes of the endoplasmic reticulum (ER) or Golgi apparatus, invariably contains two virus-specified (glyco)protein species, S and M. Glycoprotein S comprises the large surface projections, while M is a triple-spanning transmembrane protein. (wikipedia.org)
  • BioLegend's LEGEND MAX™ SARS-CoV2- Nucleocapsid Protein ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate. (biolegend.com)
  • The N protein of the related virus pneumonia virus of mice (PVM) is functionally unable to replace the RSV N protein in a minigenome replication assay. (microbiologyresearch.org)
  • The recombinant nucleocapsid (N) protein of RVF virus was tested for diagnostic applicability in an indirect enzyme-linked immunosorbent assay (I-ELISA), using sera from experimentally infected sheep (n = 128), vaccinated sheep (n = 240), and field-collected sera from sheep (n = 251), goats (n = 362) and cattle (n = 100). (up.ac.za)
  • They were characterized by (i) their activity in the BUNV minigenome system, (ii) their activity in BUNV packaging assay, (iii) their ability to form multimers, (iv) their ability to interact with L protein, and (v) their impact on RNA synthesis. (st-andrews.ac.uk)
  • Non-disulfide-mediated N protein interactions were subsequently demonstrated both in vitro by the glutathione S-transferase (GST) pull-down assay and in vivo by the mammalian two-hybrid assay. (illinois.edu)
  • RESULTS We developed a fluorescence immunochromatographic assay for detecting nucleocapsid protein of SARS-CoV-2 in nasopharyngeal swab sample and urine within 10 minutes. (cdc.gov)
  • CONCLUSIONS Those findings indicate that nucleocapsid protein assay is an accurate, rapid, early and simple method for diagnosis of COVID-19. (cdc.gov)
  • We determined the NMR structure of the N-terminal domain (residues 1-74, called N1-74) of the Andes hantavirus N protein. (ku.edu)
  • Synthetic peptides were made to overlap amino acid residues (aa 414 to 524) in the carboxy terminus of RPV N protein. (asm.org)
  • Using chimeric proteins, in which the immediate C-terminal part of the RSV N protein was replaced with the equivalent region of the PVM N protein, it was shown that six amino acid residues near the C terminus of the N protein (between residues 352-369) are essential for its function in replication and for the ability of the N protein to bind to the viral phosphoprotein, P. (microbiologyresearch.org)
  • The high affinity binding of pp12 to viral RNA can be restored by phosphorylation of the protein with a protein kinase, protease-activated kinase I. The same serine residues phosphorylated in vivo are phosphorylated by protease kinase I in vitro. (northwestern.edu)
  • The recombinant (r)NTD protein consists of 336 amino acid (aa) residues (18-353) of S protein. (bioz.com)
  • Mutational analysis indicated that of the three conserved cysteine residues in the N protein, only the cysteine at position 23 was involved in the formation of disulfide linkages. (illinois.edu)
  • The severe acute respiratory syndrome coronavirus nucleocapsid protein (SARS-CoV NP), a phosphoprotein of 48 kDa, is the most abundant protein in the virus-infected cells. (biovendor.com)
  • Protein interaction domains of the measles virus nucleocapsid protein (NP) Liston, P. (deepdyve.com)
  • It has been shown that the N proteins from the measles and rabies viruses form helical nucleocapsid-like structures when expressed in bacteria (Schoehn et al. (tamu.edu)
  • Electron microscopy image of purified yeast-expressed measles nucleocapsid protein product. (baltymas.lt)
  • BALTYMAS produces a range of human virus nucleocapsid proteins including measles, mumps, respiratory syncytial virus, different types of parainfluenza and influenza virus. (baltymas.lt)
  • Measles protein can be used in ELISA and Western Blots. (acris-antibodies.com)
  • Measles virus nucleocapsid protein modulates the Signal Regulatory Pro" by Kumaran Sundaram, Yuvaraj Sambandam et al. (henryford.com)
  • Measles virus nucleocapsid protein modulates the Signal Regulatory Protein-β1 (SIRPβ1) to enhance osteoclast differentiation in Paget's disease of bone. (henryford.com)
  • Measles virus nucleocapsid protein modulates the Signal Regulatory Protein-beta1 (SIRPbeta1) to enhance osteoclast differentiation in Paget's disease of bone. (henryford.com)
  • Environmental factors such as paramyxovirus are implicated in PDB and measles virus nucleocapsid protein (MVNP) has been shown to induce pagetic osteoclasts (OCLs). (henryford.com)
  • The nucleocapsid consisting of a protein shell and viral nucleic acids has a helical symmetry. (wikipedia.org)
  • Most notably there is a region of potential T cell cross-reactivity within the SARS-CoV-2 N protein position 102-110 amino acids that traverses multiple human alpha and betacoronaviruses. (frontiersin.org)
  • Cross-reactivity: HSV2 nuclear protein. (lsbio.com)
  • Reactivity of full-length, amino-terminal, and carboxy-terminal recombinant N proteins with hyperimmune RPV or PPRV sera in Western immunoblotting. (asm.org)
  • These results suggest that the HTNV circumvents host antiviral signaling and apoptotic response mediated by the TNFR pathway through host interactions with the N protein. (nih.gov)
  • however, the molecular determinants that govern the interactions and functions of these three proteins are still unknown. (nature.com)
  • In summary, BUNV N protein was shown to be multi-functional and involved in the regulation of virus transcription and replication, RNA synthesis and assembly, via interactions with the viral L polymerase, RNA backbone, itself or the viral glycoproteins. (st-andrews.ac.uk)
  • The N protein dimer was shown to be stable both in the presence and absence of intermolecular disulfide linkages, indicating that noncovalent interactions also play a role in dimerization. (illinois.edu)
  • Using a series of N protein deletion mutants fused to GST, amino acids 30 to 37 were shown to be essential for N-N interactions. (illinois.edu)
  • Furthermore, since RNase A treatment markedly decreased N protein-binding affinity, it appears that at least in vitro, RNA may be involved in bridging N-N interactions. (illinois.edu)
  • The recombinant nucleocapsid protein ELISA method might be helpful for bovine coronavirus diagnosis and surveillance. (scitechnol.com)
  • In order to capture the IgM and IgG induced by SARS-CoV-2 , the recombinant nucleocapsid protein of SARS-CoV-2 was successfully expressed from E. coli. (news-medical.net)
  • The coronavirus nucleocapsid protein (N) encapsidates the genomic RNA to form a helical nucleocapsid. (elsevier.com)
  • The N protein packages the viral genomic RNA to form the helical nucleocapsid that is incorporated into the budding particle but also fulfills additional roles during the viral infection. (sinobiological.com)
  • abstract = "The major nucleocapsid protein of avian retroviruses, pp12, preferentially binds to the single-stranded regions of 60 S viral RNA with an apparent binding constant (K(app)) of 1.2 x 1011 M-1. (northwestern.edu)
  • Schematic illustration of the nucleic acids binding, fraying and annealing properties of the HIV nucleocapsid protein. (nih.gov)
  • By solving the crystal structures of the TSWV N protein with nucleic acids, we showed that an inner cleft of the asymmetric trimeric ring is an RNA-binding site. (rcsb.org)
  • This study revealed the structure of the N protein, with or without nucleic acids, of TSWV as the first virus of the genus Tospovirus , so it completed our view of the N proteins of the family Bunyaviridae . (rcsb.org)
  • Since the N protein is involved in viral RNA packaging to form a helical core, it is suffice to say that both NL63-N and CypA are possibly within the HCoV-NL63 replication/transcription complex and NL63-N/human CypA interaction might function in the regulation of HCoV-NL63 RNA synthesis. (uwc.ac.za)
  • A synthetic peptide corresponding to the center region of MERS-CoV (NCoV / Novel coronavirus) Nucleocapsid Protein (NP protein). (bioz.com)
  • In parallel experiments, the remaining mice were challenged with MERS-CoV to detect the protective effect elicited by the rNTD protein. (bioz.com)
  • Recombinant MERS-CoV Nucleocapsid His-tag Protein SDS-PAGE 2 μg/lane of Recombinant MERS-CoV Nucleocapsid His-tag Protein ( 10521-CV ) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 49-55 kDa. (bio-techne.com)
  • The MERS-CoV N protein shares 46.3% and 4.5% amino acid sequence identity with SARS-CoV-1 and SARS-CoV-2 N protein, respectively. (bio-techne.com)
  • MERS-CoV N proteins have been shown to inhibit Type I Interferon(IFN) production(1). (bio-techne.com)
  • There are 10 Nucleocapsid / NP protein produced in house with high quality which are covering various species. (sinobiological.com)
  • There are 13 kinds of tags for each SFTSV Nucleocapsid of different species, especially GFP tag, OFP tag, FLAG tag and so on. (348702.live)
  • Nucleocapsid proteins are the major antigenic determinants of infectious disease causing viruses. (baltymas.lt)
  • Pools of overlapping peptides from antigenic proteins for T-cell stimulation, T-cell expansion in T-cell assays such as ELISPOT, ICS or Flow Cytometry, e.g. for immune monitoring. (jpt.com)
  • Two forms (~50 kDa and 55 kDa) of the bovine coronavirus (BCV) N protein were detected in infected cells. (elsevier.com)
  • Hogue, BG 1995, ' Bovine coronavirus nucleocapsid protein processing and assembly ', Advances in experimental medicine and biology , vol. 380, pp. 259-263. (elsevier.com)
  • It was identified to be approximately 48 kDa as the rN protein and can bind bovine coronavirus dairy cattle positive serum by western blot analysis. (scitechnol.com)
  • Reactivities of synthetic peptides to hyperimmune RPV and N protein-specific antisera. (asm.org)
  • 1H-NMR studies with the nucleocapsid protein and analogous synthetic peptides showed that all of the compounds mentioned above have zinc-ejecting capabilities. (umaryland.edu)
  • The N-terminal zinc finger and flanking basic domains represent the minimal region of the human immunodeficiency virus type-1 nucleocapsid protein for targeting chaperone function. (semanticscholar.org)
  • Human immunodeficiency virus type 1 nucleocapsid zn(2+) fingers are required for efficient reverse transcription, initial integration processes, and protection of newly synthesized viral DNA. (semanticscholar.org)
  • The prion protein has DNA strand transfer properties similar to retroviral nucleocapsid protein. (semanticscholar.org)
  • Properties and functions of the nucleocapsid protein in virus assembly. (nih.gov)
  • Characterization of expression of Puumala virus nucleocapsid protein in transgenic plants. (nih.gov)
  • These transgenic plants expressed the N protein of Puumala virus strain CG-1820. (nih.gov)
  • Among TSWV-encoded proteins, the N protein is required for assembling the viral genomic RNA into the viral ribonucleoprotein (vRNP), which is involved in various steps of the life cycle of these viruses, such as RNA replication, virus particle formation, and cell-to-cell movement. (rcsb.org)
  • Computer model showing the hexameric structure of the toscana virus nucleocapsid protein. (sciencephoto.com)
  • Molecular model of the nucleocapsid protein (blue) from HIV-1 (human immunodeficiency virus-type 1) complexed with the Psi RNA (ribonucleic acid) packaging element (pink). (sciencephoto.com)
  • Herein, we show a direct relationship between the Hantaan virus (HTNV) nucleocapsid (N) protein and the modulation of apoptosis. (nih.gov)
  • Among these proteins, the HIV-1 nucleocapsid protein (NC) is a highly conserved protein in diverse HIV subtypes that plays a central role in virus replication. (europa.eu)
  • The NCps (nucleocapsid proteins) of HIV-1 (HIV type 1), HIV-2 and SIV (simian immunodeficiency virus) are small highly basic proteins, characterized by the presence of two CCHC ZF (zinc finger) domains. (archives-ouvertes.fr)
  • Leonard, V.H.J. , Kohl, A. , Osborne, J.C. , McLees, A. and Elliott, R.M. (2005) Homotypic interaction of Bunyamwera virus nucleocapsid protein. (gla.ac.uk)
  • Herpes Simplex Virus I (HSV I), Nucleocapsid Protein, 155kD. (lsbio.com)
  • Results from two other forms of HIV-1 NC (NCp9 and NCp15) and NC proteins from Simian Immunodeficiency Virus and Murine Leukemia Virus are also reported. (umd.edu)
  • A chimeric protein composed of the 369-389 peptide substituted for the NLS of equine infectious anemia virus (EIAV) Rev protein (ERev) showed no nuclear localization activity. (k-state.edu)
  • The nucleocapsid (NC) protein NCp7 of the immunodeficiency virus type 1 is a small basic protein with two zinc finger motifs. (semanticscholar.org)
  • Because phosphorylation of the infectious bronchitis virus (IBV) nucleocapsid (N) protein may regulate its multiple roles in viral replication, the dynamics of N phosphorylation were examined. (tamu.edu)
  • Some laboratories have demonstrated that this protein interferes with different cellular pathways, thus implying it to be a key regulatory component of the virus (Zakhartchouk, Viswanathan et al. (uwc.ac.za)
  • A method for purifying the nucleocapsid protein of Nipah virus. (upm.edu.my)
  • The method leads to the recovery of the nucleocapsid of Nipah virus in high yields, biologically active and free from other host cell proteins. (upm.edu.my)
  • Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. (sinobiological.com)
  • The SARS-COV-2 nucleocapsid protein (N protein) is the most abundant protein within the virus particle. (sanyoubio.com)
  • Because of the strong immunogenicity of coronavirus Nucleocapsid, it is believed that SARS-CoV-2 Nucleocapsid Protein has potential value for the diagnosis of the virus. (elisakitgs.com)
  • The human immunodeficiency virus type-1 (HIV-1) nucleocapsid protein (NC) is derived from the Gag polyprotein precursor by the viral protease during viral assembly [ 1 ]. (biomedcentral.com)
  • As a step toward understanding the assembly pathway of the porcine reproductive and respiratory syndrome virus (PRRSV), the oligomeric properties of the nucleocapsid (N) protein were investigated. (illinois.edu)
  • Pulse-chase analysis revealed that N protein homodimers become specifically disulfide linked within the virus-infected cell, albeit at the later stages of infection, conceivably when the virus particle buds into the oxidizing environment of the endoplasmic reticulum. (illinois.edu)
  • Wootton, SK & Yoo, D 2003, ' Homo-oligomerization of the porcine reproductive and respiratory syndrome virus nucleocapsid protein and the role of disulfide linkages ', Journal of virology , vol. 77, no. 8, pp. 4546-4557. (illinois.edu)
  • Consistent with the G0/G1-phase accumulation, the amount of hyperphosphorylated retinoblastoma protein, a necessary active form for cell cycle progression through late G1 into S phase, decreased after infection with A/WSN/33 (H1N1) virus. (psu.edu)
  • In addition, SARS-CoV-2 Nucleocapsid protein has the potential to become the diagnosis target of the virus due to its strong immunogenicity. (bonopusbio.com)
  • The pneumovirus M2-1 protein is distinctive, and no homologue has been found in any other virus families. (wikipedia.org)
  • Furthermore, recombinant MeV lacking the La-binding motif in the N-5′UTR displayed delayed viral protein synthesis and growth kinetics at an early phase of infection. (frontiersin.org)
  • Among these Nucleocapsid / NP proteins, there are 3 H1N1 Nucleocapsid / NP protein, 1 H2N2 Nucleocapsid / NP protein, 2 H7N9 Nucleocapsid / NP protein, 3 H3N2 Nucleocapsid / NP protein, 1 Influenza B Nucleocapsid / NP protein. (sinobiological.com)
  • The major nucleocapsid protein of avian retroviruses, pp12, preferentially binds to the single-stranded regions of 60 S viral RNA with an apparent binding constant (K(app)) of 1.2 x 10 11 M -1 . (northwestern.edu)
  • The N protein encapsidates all three segments to form transcriptionally active ribonucleoproteins (RNPs). (st-andrews.ac.uk)
  • Because of the conservation of the N protein sequence, the expanding knowledge of its genetics and biochemistry, and its strong immunogenicity, the N protein of SARS-CoV-2 should be strongly considered as a vaccine candidate for SARS-CoV-2. (asm.org)
  • Destabilization of the HIV-1 complementary sequence of TAR by the nucleocapsid protein through activation of conformational fluctuations. (semanticscholar.org)
  • HIV-1 nucleocapsid protein activates transient melting of least stable parts of the secondary structure of TAR and its complementary sequence. (semanticscholar.org)
  • Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. (sinobiological.com)
  • This recombinant protein is isolated from HEK293 cells expressing a DNA sequence encoding SARS-CoV2 (COVID-19) NP and a polyhistidine-SUMO tag at the N-terminus. (cellecta.com)
  • In total, 102 single amino acid substitutions were generated in the BUNV N protein sequence. (st-andrews.ac.uk)
  • In the present study, the expression of the nucleocapsid (N) protein of hantavirus serotype Puumala in tobacco and potato plants was investigated. (nih.gov)
  • Although strategies to fight DR may span from the development of an effective vaccine to target host proteins essential for HIV-1 replication, the most commonly and realistic adopted strategy is still the design of potent and selective inhibitors of target proteins. (europa.eu)
  • In this context, the objective of the THINPAD project is to discover and develop novel anti-HIV-1 agents targeting the HIV-1 Nucleocapsid protein (NC), which is one of the most conserved sequences within HIV strains and is highly required for HIV-1 replication, being therefore a primary target to overcome antiretroviral DR. (europa.eu)
  • Not only does this study underscore the critical role that these proteins play in the viral replication cycle, but it also identifies a key interaction interface on VP24 that may serve as a novel target for antiviral therapeutic intervention. (nature.com)
  • This study is the first to identify how VP24 interacts with NP, and it underscores the critical role that these proteins play in the EBOV replication cycle. (nature.com)
  • The highly conserved and mutationally intolerant retroviral zinc finger motif of the HIV-1 nucleocapsid protein is an attractive target for drug therapy due to its participation in multiple stages of the viral replication cycle. (umaryland.edu)
  • Prior to undergoing replication, mRNA is transcribed and viral proteins are translated. (wikipedia.org)
  • Numbers following R indicate the corresponding position of the first amino acid residue of each peptide in the native N protein. (asm.org)
  • a The amino acid position in the native N protein corresponding to the first amino acid of the peptide is indicated in parentheses. (asm.org)
  • It was demonstrated that single amino acid substitutions could alter N protein electrophoretic mobility in SDS- PAGE (e.g. (st-andrews.ac.uk)
  • These findings increase our understanding of the Ntail of NP protein and the virulence factors associated with PPMV-1. (microbiologyresearch.org)
  • The predicted molecular weight of Recombinant SARS-CoV-2, Nucleocapsid (N) Protein is Mr 47 kDa. (prosci-inc.com)
  • However, the complex molecular details of viral entry may lead to complications with the vaccine response, similar to those seen with HIV type 1 (HIV-1) Env protein vaccine efforts ( 10 ). (asm.org)
  • Thus, Arg22 and Lys26 are likely involved in a conformational change or molecular recognition when the N protein is trafficked from the cytoplasm to the Golgi, the site of viral assembly and maturation. (ku.edu)
  • Indeed, the molecular determinants that govern the interaction between these two proteins are unknown, as is the precise functional relevance of the interaction. (nature.com)
  • No. NUN-S95) was more than 85% and the molecular weight of this protein is around 135-145 kDa verified by SEC-MALS. (acrobiosystems.com)
  • The apparent molecular mass of the protein is approximately 57 kDa in SDS-PAGE under reducing conditions. (sinobiological.com)