Nucleic acid hybridization. Medical search

Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)

Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.

Deoxyribonucleic acid that makes up the genetic material of viruses.

Ribonucleic acid that makes up the genetic material of viruses.

A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).

The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.

Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible.

A genus of RETROVIRIDAE comprising endogenous sequences in mammals, related RETICULOENDOTHELIOSIS VIRUSES, AVIAN, and a reptilian virus. Many species contain oncogenes and cause leukemias and sarcomas.

Deoxyribonucleic acid that makes up the genetic material of bacteria.

High molecular weight polymers containing a mixture of purine and pyrimidine nucleotides chained together by ribose or deoxyribose linkages.

DNA analogs containing neutral amide backbone linkages composed of aminoethyl glycine units instead of the usual phosphodiester linkage of deoxyribose groups. Peptide nucleic acids have high biological stability and higher affinity for complementary DNA or RNA sequences than analogous DNA oligomers.

Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.

A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.

A series of steps taken in order to conduct research.

Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.

Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)

A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.

Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).

Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.

The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.

In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.

The functional hereditary units of VIRUSES.

An enzyme that synthesizes DNA on an RNA template. It is encoded by the pol gene of retroviruses and by certain retrovirus-like elements. EC 2.7.7.49.

Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.

The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.

Substances elaborated by viruses that have antigenic activity.

The genetic process of crossbreeding between genetically dissimilar parents to produce a hybrid.

A membrane or barrier with micrometer sized pores used for separation purification processes.

The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.

Established cell cultures that have the potential to propagate indefinitely.

A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)

Nucleic acid which complements a specific mRNA or DNA molecule, or fragment thereof; used for hybridization studies in order to identify microorganisms and for genetic studies.

RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.

Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.

A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.

Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.

Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)

The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.

Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)

Presence of warmth or heat or a temperature notably higher than an accustomed norm.

The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.

The rate dynamics in chemical or physical systems.

The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies.

A method for comparing two sets of chromosomal DNA by analyzing differences in the copy number and location of specific sequences. It is used to look for large sequence changes such as deletions, duplications, amplifications, or translocations.

RNA, usually prepared by transcription from cloned DNA, which complements a specific mRNA or DNA and is generally used for studies of virus genes, distribution of specific RNA in tissues and cells, integration of viral DNA into genomes, transcription, etc. Whereas DNA PROBES are preferred for use at a more macroscopic level for detection of the presence of DNA/RNA from specific species or subspecies, RNA probes are preferred for genetic studies. Conventional labels for the RNA probe include radioisotope labels 32P and 125I and the chemical label biotin. RNA probes may be further divided by category into plus-sense RNA probes, minus-sense RNA probes, and antisense RNA probes.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.

The relationships of groups of organisms as reflected by their genetic makeup.

Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.

Identification and characterization of the human orthologue of yeast Pex14p. (1/18626)

Pex14p is a central component of the peroxisomal protein import machinery, which has been suggested to provide the point of convergence for PTS1- and PTS2-dependent protein import in yeast cells. Here we describe the identification of a human peroxisome-associated protein (HsPex14p) which shows significant similarity to the yeast Pex14p. HsPex14p is a carbonate-resistant peroxisomal membrane protein with its C terminus exposed to the cytosol. The N terminus of the protein is not accessible to exogenously added antibodies or protease and thus might protrude into the peroxisomal lumen. HsPex14p overexpression leads to the decoration of tubular structures and mislocalization of peroxisomal catalase to the cytosol. HsPex14p binds the cytosolic receptor for the peroxisomal targeting signal 1 (PTS1), a result consistent with a function as a membrane receptor in peroxisomal protein import. Homo-oligomerization of HsPex14p or interaction of the protein with the PTS2-receptor or HsPex13p was not observed. This distinguishes the human Pex14p from its counterpart in yeast cells and thus supports recent data suggesting that not all aspects of peroxisomal protein import are conserved between yeasts and humans. The role of HsPex14p in mammalian peroxisome biogenesis makes HsPEX14 a candidate PBD gene for being responsible for an unrecognized complementation group of human peroxisome biogenesis disorders. (+info)

The LIM-only protein PINCH directly interacts with integrin-linked kinase and is recruited to integrin-rich sites in spreading cells. (2/18626)

PINCH is a widely expressed and evolutionarily conserved protein comprising primarily five LIM domains, which are cysteine-rich consensus sequences implicated in mediating protein-protein interactions. We report here that PINCH is a binding protein for integrin-linked kinase (ILK), an intracellular serine/threonine protein kinase that plays important roles in the cell adhesion, growth factor, and Wnt signaling pathways. The interaction between ILK and PINCH has been consistently observed under a variety of experimental conditions. They have interacted in yeast two-hybrid assays, in solution, and in solid-phase-based binding assays. Furthermore, ILK, but not vinculin or focal adhesion kinase, has been coisolated with PINCH from mammalian cells by immunoaffinity chromatography, indicating that PINCH and ILK associate with each other in vivo. The PINCH-ILK interaction is mediated by the N-terminal-most LIM domain (LIM1, residues 1 to 70) of PINCH and multiple ankyrin (ANK) repeats located within the N-terminal domain (residues 1 to 163) of ILK. Additionally, biochemical studies indicate that ILK, through the interaction with PINCH, is capable of forming a ternary complex with Nck-2, an SH2/SH3-containing adapter protein implicated in growth factor receptor kinase and small GTPase signaling pathways. Finally, we have found that PINCH is concentrated in peripheral ruffles of cells spreading on fibronectin and have detected clusters of PINCH that are colocalized with the alpha5beta1 integrins. These results demonstrate a specific protein recognition mechanism utilizing a specific LIM domain and multiple ANK repeats and suggest that PINCH functions as an adapter protein connecting ILK and the integrins with components of growth factor receptor kinase and small GTPase signaling pathways. (+info)

Isolation of human transcripts expressed in hamster cells from YACs by cDNA representational difference analysis. (3/18626)

Gene isolation methods used during positional cloning rely on physical contigs consisting of bacterial artificial chromosomes, P1, or cosmid clones. However, in most instances, the initial framework for physical mapping consists of contigs of yeast artificial chromosome (YACs), large vectors that are suboptimal substrates for gene isolation. Here we report a strategy to identify gene sequences contained within a YAC by using cDNA representational difference analysis (RDA) to directly isolate transcripts expressed from the YAC in mammalian cells. The RDA tester cDNAs were generated from a previously reported hamster cell line derived by stable transfer of a 590-kb YAC (911D5) that expressed NPC1, the human gene responsible for Niemann-Pick type C (NP-C). The driver cDNAs were generated from a control hamster cell line that did not contain the YAC that expressed NPC1. Among the gene fragments obtained by RDA, NPC1 was the most abundant product. In addition, two non-NPC1 fragments were isolated that were mapped to and expressed from 911D5. One of these RDA gene fragments (7-R) spans more than one exon and has 98% sequence identity with a human cDNA clone reported previously as an expressed sequence tag (EST), but not mapped to a chromosomal region. The other fragment (2-R) that had no significant sequence similarities with known mammalian genes or ESTs, was further localized to the region of overlap between YACs 911D5 and 844E3. The latter YAC is part of a contig across the NP-C candidate region, but does not contain NPC1. This two-part approach in which stable YAC transfer is followed by cDNA RDA should be a useful adjunct strategy to expedite the cloning of human genes when a YAC contig is available across a candidate interval. (+info)

Single atom modification (O-->S) of tRNA confers ribosome binding. (4/18626)

Escherichia coli tRNALysSUU, as well as human tRNALys3SUU, has 2-thiouridine derivatives at wobble position 34 (s2U*34). Unlike the native tRNALysSUU, the full-length, unmodified transcript of human tRNALys3UUU and the unmodified tRNALys3UUU anticodon stem/loop (ASLLys3UUU) did not bind AAA- or AAG-programmed ribosomes. In contrast, the completely unmodified yeast tRNAPhe anticodon stem/loop (ASLPheGAA) had an affinity (Kd = 136+/-49 nM) similar to that of native yeast tRNAPheGmAA (Kd = 103+/-19 nM). We have found that the single, site-specific substitution of s2U34 for U34 to produce the modified ASLLysSUU was sufficient to restore ribosomal binding. The modified ASLLysSUU bound the ribosome with an affinity (Kd = 176+/-62 nM) comparable to that of native tRNALysSUU (Kd = 70+/-7 nM). Furthermore, in binding to the ribosome, the modified ASLLys3SUU produced the same 16S P-site tRNA footprint as did native E. coli tRNALysSUU, yeast tRNAPheGmAA, and the unmodified ASLPheGAA. The unmodified ASLLys3UUU had no footprint at all. Investigations of thermal stability and structure monitored by UV spectroscopy and NMR showed that the dynamic conformation of the loop of modified ASLLys3SUU was different from that of the unmodified ASLLysUUU, whereas the stems were isomorphous. Based on these and other data, we conclude that s2U34 in tRNALysSUU and in other s2U34-containing tRNAs is critical for generating an anticodon conformation that leads to effective codon interaction in all organisms. This is the first example of a single atom substitution (U34-->s2U34) that confers the property of ribosomal binding on an otherwise inactive tRNA. (+info)

Suppression subtractive hybridization identifies high glucose levels as a stimulus for expression of connective tissue growth factor and other genes in human mesangial cells. (5/18626)

Accumulation of mesangial matrix is a pivotal event in the pathophysiology of diabetic nephropathy. The molecular triggers for matrix production are still being defined. Here, suppression subtractive hybridization identified 15 genes differentially induced when primary human mesangial cells are exposed to high glucose (30 mM versus 5 mM) in vitro. These genes included (a) known regulators of mesangial cell activation in diabetic nephropathy (fibronectin, caldesmon, thrombospondin, and plasminogen activator inhibitor-1), (b) novel genes, and (c) known genes whose induction by high glucose has not been reported. Prominent among the latter were genes encoding cytoskeleton-associated proteins and connective tissue growth factor (CTGF), a modulator of fibroblast matrix production. In parallel experiments, elevated CTGF mRNA levels were demonstrated in glomeruli of rats with streptozotocin-induced diabetic nephropathy. Mannitol provoked less mesangial cell CTGF expression in vitro than high glucose, excluding hyperosmolality as the key stimulus. The addition of recombinant CTGF to cultured mesangial cells enhanced expression of extracellular matrix proteins. High glucose stimulated expression of transforming growth factor beta1 (TGF-beta1), and addition of TGF-beta1 to mesangial cells triggered CTGF expression. CTGF expression induced by high glucose was partially suppressed by anti-TGF-beta1 antibody and by the protein kinase C inhibitor GF 109203X. Together, these data suggest that 1) high glucose stimulates mesangial CTGF expression by TGFbeta1-dependent and protein kinase C dependent pathways, and 2) CTGF may be a mediator of TGFbeta1-driven matrix production within a diabetic milieu. (+info)

Human geranylgeranyl diphosphate synthase. cDNA cloning and expression. (6/18626)

Geranylgeranyl diphosphate (GGPP) synthase (GGPPSase) catalyzes the synthesis of GGPP, which is an important molecule responsible for the C20-prenylated protein biosynthesis and for the regulation of a nuclear hormone receptor (LXR.RXR). The human GGPPSase cDNA encodes a protein of 300 amino acids which shows 16% sequence identity with the known human farnesyl diphosphate (FPP) synthase (FPPSase). The GGPPSase expressed in Escherichia coli catalyzes the GGPP formation (240 nmol/min/mg) from FPP and isopentenyl diphosphate. The human GGPPSase behaves as an oligomeric molecule with 280 kDa on a gel filtration column and cross-reacts with an antibody directed against bovine brain GGPPSase, which differs immunochemically from bovine brain FPPSase. Northern blot analysis indicates the presence of two forms of the mRNA. (+info)

The biosynthesis of transfer RNA in insects. II. Isolation of transfer RNA precursors from the posterior silk gland of Bombyx mori. (7/18626)

The occurrence of precursors to tRNA in the post-polysomal fraction of the posterior silk gland of Bombyx mori was demonstrated by pulse-chase labeling and DNA-RNA hybridization competition experiments. These precursors had molecular sizes ranging from 4S to 5S on polyacrylamide gel electrophoresis. Analysis of the incorporation of the methyl group from [methyl-14C]methionine revealed that a radioactive peak on polyacrylamide gel appeared in the 4.5S region during brief labeling. This suggested that some methylation occurred at the 4.5S precursor step. (+info)

Burkholderia cocovenenans (van Damme et al. 1960) Gillis et al. 1995 and Burkholderia vandii Urakami et al. 1994 are junior synonyms of Burkholderia gladioli (Severini 1913) Yabuuchi et al. 1993 and Burkholderia plantarii (Azegami et al. 1987) Urakami et al. 1994, respectively. (8/18626)

Reference strains of Burkholderia cocovenenans and Burkholderia vandii were compared with strains of other Burkholderia species using SDS-PAGE of whole-cell proteins, DNA-DNA hybridization and extensive biochemical characterization. Burkholderia gladioli and B. cocovenenans were indistinguishable in the chemotaxonomic and biochemical analyses. Burkholderia plantarii and B. vandii had indistinguishable whole-cell protein patterns but the B. vandii type strain differed from B. plantarii strains in several biochemical tests. The DNA-DNA binding levels (higher than 70%) indicated that (i) B. gladioli and B. cocovenenans, and (ii) B. plantarii and B. vandii each represent a single species. It is concluded that B. cocovenenans and B. vandii are junior synonyms of B. gladioli and B. plantarii, respectively. (+info)

"Nucleic Acid Hybridizations". DNA - Basics of Structure and Analysis. Retrieved 26 May 2017. Beckman, Mary. "Hybridization". ... In molecular biology, hybridization (or hybridisation) is a phenomenon in which single-stranded deoxyribonucleic acid (DNA) or ... Felsenfeld, G; Miles, HT (1967). "The physical and chemical properties of nucleic acids". Annual Review of Biochemistry. 36: ... Southern hybridization & Northern hybridization (Articles needing additional references from February 2015, All articles ...

https://en.wikipedia.org/wiki/Nucleic_acid_hybridization

... the quantitative annealing of two complementary strands of nucleic acids, known as nucleic acid hybridization. In the context ... in the case of nucleic acid hybridization, monovalent salt concentration and temperature are controlled for hybridization and ... In the sandwich hybridization ELISA assay format, the antigen ligand and antibodies in ELISA are replaced with a nucleic acid ... "Nucleic Acids: Hybridisation". wiley. Retrieved 4 February 2017. Efler, S.M.; Zhang, L.; Noll, B.O.; Uhlmann, E.; Davis, H.L. ( ...

https://en.wikipedia.org/wiki/Hybridization_assay

"Nucleic Acid Hybridization - MeSH - NCBI". www.ncbi.nlm.nih.gov. Retrieved 16 November 2020. Wang, Qiaochun; Mawassi, Munir; Li ... The fifth ORF codes for nucleic-acid binding protein. This is the protein that helps the DNA or RNA connect with amino acids. ... Nucleic acid hybridizationis also used to detect GVA. In this method, a small sample of the infected plant is taken and then ... "Nucleic Acid Binding Protein - an overview , ScienceDirect Topics". www.sciencedirect.com. Retrieved 16 November 2020. "RPO132 ...

https://en.wikipedia.org/wiki/Grapevine_virus_A

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https://en.wikipedia.org/wiki/Hybridization_probe

In 2012, Yin's research group published a paper about optimizing the specificity of nucleic acid hybridization. They introduced ... A nucleic acid test (NAT) is a technique used to detect a particular nucleic acid sequence and thus usually to detect and ... Peng Yin, David Zhang (2012). "Optimizing the specificity of nucleic acid hybridization". Nature Chemistry. 4 (3): 208-214. ... Nucleic acid tests use a "probe" which is a long strand with a short strand stuck to it. The long primer strand has a ...

https://en.wikipedia.org/wiki/Nucleic_acid_test

Markham NR, Zuker M (2008). UNAFold: software for nucleic acid folding and hybridization. Methods in Molecular Biology. Vol. ... "Sfold web server for statistical folding and rational design of nucleic acids". Nucleic Acids Research. 32 (Web Server issue): ... software to identify motifs and short-range interactions in trajectories of nucleic acids". Nucleic Acids Research. 43 (17): ... Wilm A, Higgins DG, Notredame C (May 2008). "R-Coffee: a method for multiple alignment of non-coding RNA". Nucleic Acids ...

https://en.wikipedia.org/wiki/List_of_RNA_structure_prediction_software

Markham NR, Zuker M (2008). UNAFold: software for nucleic acid folding and hybridization. Methods in Molecular Biology. Vol. ... Nucleic Acids Research. 25 (17): 3389-3402. doi:10.1093/nar/25.17.3389. PMC 146917. PMID 9254694. Yi Z, Ouyang J, Sun W, Li S, ... Nucleic Acids Research. 45 (W1): W98-W102. doi:10.1093/nar/gkx247. PMC 5570223. PMID 28407145. Cerami E, Gao J, Dogrusoz U, ... Nucleic Acids Research. 43 (Database issue): D512-D520. doi:10.1093/nar/gku1267. PMC 4383998. PMID 25514926. Altschul SF, ...

https://en.wikipedia.org/wiki/CCDC188

Noyes, Barbara E.; Stark, George R. (July 1975). "Nucleic acid hybridization using DNA covalently coupled to cellulose". Cell. ... and thus crucial for oxidizing ascorbic acid. Through inhibition studies with p-chloro-mercuribenzoic acid, Stark came to the ... Essentially, cyanate reacts with the amino groups and exposes them in order for them to react with acid and form hydantoins, ... Using chromatography, Stark was able to detect a change in the amino acid sequence of ribonuclease, specifically the loss of ...

https://en.wikipedia.org/wiki/George_Stark

Web server for nucleic acid folding and hybridization prediction. Retrieved on 2020-8-01. RBPmap: mapping binding sites of RNA ... December 2014). "Chk2 and REGγ-dependent DBC1 regulation in DNA damage induced apoptosis". Nucleic Acids Research. 42 (21): ... Isoform 1 has 199 amino acid residues and a domain named DUF4517. Isoform 2 has 174 amino acid residues, and isoform X1 has 154 ... Overall, the positively charged amino acid residues in human protein C20orf27 outnumbers the negatively charged amino acid ...

https://en.wikipedia.org/wiki/C20orf27

Zuker M (2003). "Mfold web server for nucleic acid folding and hybridization prediction". Nucleic Acids Research. 31 (13): 3406 ... "Modeling Unusual Nucleic Acid Structures". Modeling unusual nucleic acid structures. In Molecular Modeling of Nucleic Acids. ... Nucleic acid structure prediction is a computational method to determine secondary and tertiary nucleic acid structure from its ... Nucleic Acids Research, 32(Web Server issue), W142-145. Touzet H (2007). Comparative analysis of RNA genes: the caRNAc software ...

https://en.wikipedia.org/wiki/Nucleic_acid_structure_prediction

Zuker, M (2003). "Mfold web server for nucleic acid folding and hybridization prediction". Nucleic Acids Research. 13 (31): ... Nucleic Acids Research. 45 (D1): D200-D203. doi:10.1093/nar/gkw1129. PMC 5210587. PMID 27899674. "Predict Location of ... Chou, P. Y.; Fasman, G. D. (1978). "Prediction of the secondary structure of proteins from their amino acid sequence". Advances ... The longest polypeptide of transmembrane protein 217 consists of 229 amino acids. This protein isoform has a predicted weight ...

https://en.wikipedia.org/wiki/Transmembrane_protein_217

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https://en.wikipedia.org/wiki/C7orf25

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https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_sRNA

Zuker, M. (1 July 2003). "Mfold web server for nucleic acid folding and hybridization prediction". Nucleic Acids Research. 31 ( ... Ferre, F.; Clote, P. (1 July 2005). "DiANNA: a web server for disulfide connectivity prediction". Nucleic Acids Research. 33 ( ... Nucleic Acids Research. 36 (Web Server): W35-W41. doi:10.1093/nar/gkn211. PMC 2447719. PMID 18442995. Luck, Katja; Kim, Dae- ... Nucleic Acids Research. 49 (D1): D344-D354. doi:10.1093/nar/gkaa977. PMC 7778928. PMID 33156333. Sigrist, Christian J. A.; de ...

https://en.wikipedia.org/wiki/TMEM211

Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res. 31 (13), 3406-15, (2003) " ... Nucleic Acids Res. 25:3389-3402. "TimeTree :: The Timescale of Life". www.timetree.org. Retrieved 2017-05-12. (Orphaned ... Amino acids serine and lysine are highly represented in the protein at a higher frequency than observed in most proteins in ... BPS : A. W. Burgess and P. K. Ponnuswamy and H. A. Sheraga, Analysis of conformations of amino acid residues and prediction of ...

https://en.wikipedia.org/wiki/Tex36

Zuker M (July 2003). "Mfold web server for nucleic acid folding and hybridization prediction". Nucleic Acids Research. 31 (13 ... July 2019). "The EMBL-EBI search and sequence analysis tools APIs in 2019". Nucleic Acids Research. 47 (W1): W636-W641. doi: ... Isoform 1 is 419 amino acids long and is the most abundant form. Isoform 2 is 225 amino acids, containing only 11 exons and a ... The amino acid composition of BZW2 has a higher amount of lysines and a lower amount of prolines in humans but a higher ...

https://en.wikipedia.org/wiki/Basic_leucine_zipper_and_W2_domain-containing_protein_2

Zuker M (July 2003). "Mfold web server for nucleic acid folding and hybridization prediction". Nucleic Acids Research. 31 (13 ... Zhang Y, Skolnick J (2005-04-11). "TM-align: a protein structure alignment algorithm based on the TM-score". Nucleic Acids ... January 2020). "ELM-the eukaryotic linear motif resource in 2020". Nucleic Acids Research. 48 (D1): D296-D306. doi:10.1093/nar/ ... Yang J, Zhang Y (July 2015). "I-TASSER server: new development for protein structure and function predictions". Nucleic Acids ...

https://en.wikipedia.org/wiki/SLC46A3

2008 using fluorescence in situ hybridization and peptide nucleic acids. They developed and employed this technique for ... In molecular biology, ligation is the joining of two nucleic acid fragments through the action of an enzyme. It is an essential ... Nucleic Acids Research. 9 (1): 85-93. doi:10.1093/nar/9.1.85. PMC 326670. PMID 6259621. Dugaiczyk A, Boyer HW, Goodman HM (July ... Nucleic Acids Research. 13 (6): 1997-2008. doi:10.1093/nar/13.6.1997. PMC 341130. PMID 4000951. K Hayashi; M Nakazawa; Y ...

https://en.wikipedia.org/wiki/Ligation_(molecular_biology)

The common nucleic acid detection method includes direct DNA hybridization. The direct DNA hybridization approach is the ... Dunbar, Sherry A. (2006). "Applications of Luminex xMAP technology for rapid, high-throughput multiplexed nucleic acid ... Hybridization between the capture probe and the target DNA is achieved by melting and annealing complementary target DNA ... Probe-target hybridization is usually detected by optically labeled targets, which determines the relative abundance of each ...

https://en.wikipedia.org/wiki/Suspension_array_technology

Nucleic acid hybridization tests (DNA probe test) also find Chlamydia DNA. A probe test is very accurate but is not as ... A polymerase chain reaction (PCR) test is an example of a nucleic acid amplification test. This test can also be done on a ... Nucleic acid amplification tests (NAATs) tests find the genetic material (DNA) of Chlamydia bacteria. These tests are the most ... Fox, A., Rogers, J. C., Gilbart, J., Morgan, S., Davis, C. H., Knight, S., & Wyrick, P. B. (1990). Muramic acid is not ...

https://en.wikipedia.org/wiki/Chlamydia_trachomatis

Ng PS, Bergstrom DE (January 2005). "Alternative nucleic acid analogues for programmable assembly: hybridization of LNA to PNA ... Peptide nucleic acid (PNA) is an artificially synthesized polymer similar to DNA or RNA. Synthetic peptide nucleic acid ... Clicked peptide polymer Glycol nucleic acid Oligonucleotide synthesis Peptide synthesis Threose nucleic acid Nielsen PE, Egholm ... Zhao XL, Chen BC, Han JC, Wei L, Pan XB (November 2015). "Delivery of cell-penetrating peptide-peptide nucleic acid conjugates ...

https://en.wikipedia.org/wiki/Peptide_nucleic_acid

"Implications of High-Affinity Hybridization by Locked Nucleic Acid Oligomers for Inhibition of Human Telomerase †". ... A locked nucleic acid (LNA), also known as bridged nucleic acid (BNA), and often referred to as inaccessible RNA, is a modified ... Kurreck, J. (2002-05-01). "Design of antisense oligonucleotides stabilized by locked nucleic acids". Nucleic Acids Research. 30 ... C-Ethylene-bridged nucleic acids (ENA) with nuclease-resistance and high affnity for RNA". Nucleic Acids Symposium Series. 1 (1 ...

https://en.wikipedia.org/wiki/Locked_nucleic_acid

His work played a pivotal role in the discovery of nucleic acid hybridization. In 1955, Rich and Crick solved the structure of ... the importance of RNA and the development of nucleic acid hybridization A Conversation with Alex Rich (10/03/2007) Archived 21 ... the importance of RNA and the development of nucleic acid hybridization". MIT Department of Biology. 31 May 2018. Retrieved 21 ... The Discovery of Polynucleotide Hybridization Archived 12 November 2014 at the Wayback Machine MIT Article: Alexander Rich, ...

https://en.wikipedia.org/wiki/Alexander_Rich

Khodakov D, Wang C, Zhang DY (October 2016). "Diagnostics based on nucleic acid sequence variant profiling: PCR, hybridization ... For example, because cell-free nucleic acids exist in human plasma, a simple blood sample can be enough to sample genetic ... Tong CY, Mallinson H (May 2002). "Moving to nucleic acid-based detection of genital Chlamydia trachomatis". Expert Review of ... to vastly increase the number of nucleic acid molecules, thereby amplifying the target sequence(s) in the patient sample.: ...

https://en.wikipedia.org/wiki/Molecular_diagnostics

This dark tonality only appears when nucleic acid hybridisation occurs. Coconut cadang-cadang disease has no treatment yet. ... The first step is the purification to obtain the nucleic acids of the plant cells. The leaves of the plant located four or more ... Coconuts from Asia and South Pacific have been found to have viroids with similar nucleic acid sequences of CCCVd. The ... the nucleic acids can be extracted by chloroform procedures, for example. When approximately 1 g of coconut tissue has been ...

https://en.wikipedia.org/wiki/Cadang-cadang

"Programmable release of multiple protein drugs from aptamer-functionalized hydrogels via nucleic acid hybridization". Journal ... Chemical modifications of nucleic acid bases or backbones increase the chemical diversity of standard nucleic acid bases. The ... "Splitting aptamers and nucleic acid enzymes for the development of advanced biosensors". Nucleic Acids Research. 48 (7): 3400- ... but the nucleic acid-based structure of aptamers, which are mostly oligonucleotides, is very different from the amino acid- ...

https://en.wikipedia.org/wiki/Aptamer

"Alexander Rich, the importance of RNA and the development of nucleic acid hybridization". MIT Department of Biology. 2018-05-31 ... Cohn, E.J.; Edsall, J.T. (1943). Proteins, Amino Acids and Peptides as Ions and Dipolar Ions. New York: Reinhold. Edsall, John ... Subsequently, in 1943, they published a book Proteins, Amino Acids and Peptides. This had a profound influence on the next ... "Ultraviolet difference spectra of tyrosine groups in proteins and amino acids". J. Biol. Chem. 233 (6): 1421-1428. doi:10.1016/ ...

https://en.wikipedia.org/wiki/John_Tileston_Edsall

"Label-free detection of DNA hybridization based on hydration-induced tension in nucleic acid films". Nature Nanotechnology. 3 ( ...

https://en.wikipedia.org/wiki/Montserrat_Calleja_Gómez

The virus can be detected with ELISA, immunodiffusion tests, or a nucleic acid hybridization assay. The Cymbidium mosaic virus ... "Detection and localization of viruses in orchids by tissue-print hybridization". Plant Pathology. 41 (3): 355-361. doi:10.1111/ ...

https://en.wikipedia.org/wiki/Cymbidium_mosaic_virus

... but can also arise from polyploidisation or nucleic acid hybridization. Over evolutionary time, if the function of the new ... In particular, amino acid substitutions that change the electric charge of the enzyme are simple to identify by gel ... Alternatively, if the amino acid residue that is changed is in a relatively unimportant part of the enzyme (e.g., a long way ... The enzyme is a monomer, the isoenzymes are due to the differences in the carbohydrate content (sialic acid residues). The most ...

https://en.wikipedia.org/wiki/Isozyme

Progress in Nucleic Acid Research and Molecular Biology. Vol. 53. pp. 1-78. doi:10.1016/s0079-6603(08)60142-7. ISBN ... "Localization of the gene for uridine monophosphate synthase to human chromosome region 3q13 by in situ hybridization". Genomics ... Traut TW, Jones ME (1996). Uracil metabolism--UMP synthesis from orotic acid or uridine and conversion of uracil to beta- ... In Mycobacterium tuberculosis, two of the most promising inhibitors are 2,6-dihydroxipyridine-4-carboxylic acid and 3- ...

https://en.wikipedia.org/wiki/Uridine_monophosphate_synthase

2006). "miRBase: microRNA sequences, targets and gene nomenclature". Nucleic Acids Res. 34 (90001): D140-4. doi:10.1093/nar/ ... In particular, in situ hybridisation showed an enhanced expression of miR-137 within the dentate gyrus and the molecular layer ...

https://en.wikipedia.org/wiki/MiR-137

Nucleic Acids Res. 23 (19): 3822-30. doi:10.1093/nar/23.19.3822. PMC 307297. PMID 7479023. Di Marzio P, Choe S, Ebright M, et ... of human cell cycle regulatory genes CDC25C to 5q31 and WEE1 to 11p15.3-11p15.1 by fluorescence in situ hybridization". ...

https://en.wikipedia.org/wiki/CDC25C

Vassetzky NS, Kramerov DA (January 2013). "SINEBase: a database and tool for SINE analysis". Nucleic Acids Research. 41 ( ... The genes that had high hybridization E-values were genes particularly involved in metabolic and signaling pathways. Almost all ... was found that genes which were predicted to possess V-SINEs were targeted by microRNAs with significantly higher hybridization ...

https://en.wikipedia.org/wiki/Short_interspersed_nuclear_element

J Nucleic Acids. 2012: 295719. doi:10.1155/2012/295719. PMC 3444042. PMID 22991651. "Creative Biolabs Announced Phage Display ... Sequencing services such as Database Assisted Shotgun Sequencing (DASS) technology Analysis of microarray hybridization data ...

https://en.wikipedia.org/wiki/Creative_Biolabs

"Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplification". Nucleic Acids Res. ... so that the correct hybridization can occur. And a primer sequence at the end, it is a sequence whose design varies and is what ... "Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplification". Nucleic Acids ... The method was first described in 2002 in the scientific journal Nucleic Acid Research. The first applications included the ...

https://en.wikipedia.org/wiki/Multiplex_ligation-dependent_probe_amplification

... is a molecular cytogenetic technique that uses fluorescent probes that bind to only particular parts of a nucleic acid sequence ... The hybridization signals for each probe when a nucleic abnormality is detected. Each probe for the detection of mRNA and ... Wikimedia Commons has media related to Fluorescence in situ hybridization. Fluorescent+in+Situ+Hybridization at the US National ... The process is done in 3 main procedures: tissue preparation (pre-hybridization), hybridization, and washing (post- ...

https://en.wikipedia.org/wiki/Fluorescence_in_situ_hybridization

This is also known as self-hybridization). The Nussinov-Jacobson algorithm is used to predict secondary structures and also to ... DNA code construction refers to the application of coding theory to the design of nucleic acid systems for the field of DNA- ... i.e. these algorithms satisfy some or all the design requirements for DNA oligonucleotides at the time of hybridization (which ... DNA computing requires that the self-assembly of the oligonucleotide strands happen in such a way that hybridization should ...

https://en.wikipedia.org/wiki/Coding_theory_approaches_to_nucleic_acid_design

Nucleic Acids Res. online ahead of print. doi:10.1093/nar/gkac576. PMID 35882385. The Enzymes. books.google.co.uk. 15 December ... Plaque hybridization "Explain briefly the clover leaf model of tRNA". Preservearticle.com. Retrieved June 2, 2015. Prabhakar A ... the amino acids acceptor stem, usually attaches to amino acids and such reactions are often catalyzed by a specific enzymes, ... For example, if the amino acid that attach to the end is alanine, the reaction will be catalyzed by alanine-tRNA synthase to ...

https://en.wikipedia.org/wiki/Cloverleaf_model_of_tRNA

Bhatt R, Scott B, Whitney S, Bryan RN, Cloney L, Lebedev A (1999). "Detection of nucleic acids by cycling probe technology on ... Cycling probe technology utilizes a cyclic, isothermal process that begins with the hybridization of the chimeric probe with ... Cycling probe technology makes use of a chimeric nucleic acid probe to detect the presence of a particular DNA sequence. The ... Wolcott MJ (October 1992). "Advances in nucleic acid-based detection methods". Clinical Microbiology Reviews. 5 (4): 370-86. ...

https://en.wikipedia.org/wiki/Cycling_probe_technology

... nucleic acid - nucleic acid regulatory sequence - nucleic acid repetitive sequence - nucleic acid sequence homology - nucleon ... hybridization - hydrocarbon - hydrogen - hydrogen bond - hydrogenation - hydrogen-deuterium exchange - hydrolysis - hydrolytic ... amino acid - amino acid receptor - amino acid sequence - amino acid sequence homology - aminobutyric acid - ammonia - AMPA ... It deals with the structure and function of cellular components such as proteins, carbohydrates, lipids, nucleic acids and ...

https://en.wikipedia.org/wiki/Index_of_biochemistry_articles

... dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization". PCR ... Tyagi, Sanjay; Kramer, Fred Russell (March 1996). "Molecular Beacons: Probes that Fluoresce upon Hybridization". Nature ...

https://en.wikipedia.org/wiki/Black_Hole_Quencher_1

Nucleic Acids Res. 31 (19): 5568-75. doi:10.1093/nar/gkg765. PMC 206465. PMID 14500819. Lehner B, Semple JI, Brown SE, Counsell ... to human chromosome 12q24.3 and rat chromosome 12 by somatic cell hybrid panels and fluorescence in situ hybridization". ... Nucleic Acids Res. England. 31 (19): 5568-75. doi:10.1093/nar/gkg765. PMC 206465. PMID 14500819. Popanda O, Thielmann HW (1992 ...

https://en.wikipedia.org/wiki/POLE_(gene)

Diagnostic assays using DNA hybridization are limited by the dissociation of antiparallell duplex helices. This can be improved ... "Enhanced anti-HIV-1 activity of G-quadruplexes comprising locked nucleic acids and intercalating nucleic acids". Nucleic Acids ... Twisted intercalating nucleic acid (TINA) is a nucleic acid molecule that, when added to triplex-forming oligonucleotides (TFOs ... Géci, Imrich; Filichev, Vyacheslav V; Pedersen, Erik B (2006). "Synthesis of Twisted Intercalating Nucleic Acids Possessing ...

https://en.wikipedia.org/wiki/Twisted_intercalating_nucleic_acid

The amplification refractory mutation system (ARMS)". Nucleic Acids Res. 17 (7): 2503-2516. doi:10.1093/nar/17.7.2503. PMC ... Shorter oligos have lower hybridization energies, resulting in decreased stability of the oligo to the chromosomal target. Of ... Nucleic Acids Research. 41 (22): e204. doi:10.1093/nar/gkt1075. ISSN 1362-4962. PMC 3905872. PMID 24203710. Sharan, Shyam K.; ... Nucleic Acids Research. 41 (Database issue): D605-612. doi:10.1093/nar/gks1027. ISSN 1362-4962. PMC 3531154. PMID 23143106. ...

https://en.wikipedia.org/wiki/No-SCAR_(Scarless_Cas9_Assisted_Recombineering)_Genome_Editing

Nucleic Acids Research. 24 (7): 1294-303. doi:10.1093/nar/24.7.1294. PMC 145771. PMID 8614633. Bilaud T, Brun C, Ancelin K, ... which can be visualized by a combination of a DAPI stain and fluorescence in situ hybridization (FISH) technique. TERF2 is also ... These client proteins also contain a TRFH binding motif, which consists of a conserved 6-amino acid sequence of the following ... where the amino acid Y (tyrosine) is replaced with F (phenylalanine). TERF2 has also been shown to interact with: Ku70, MRE11A ...

https://en.wikipedia.org/wiki/Telomeric_repeat-binding_factor_2

MNase-seq and DNase-seq both follow the same principles, as they employ lytic enzymes that target nucleic acids to cut the DNA ... The DNA hybridization technique used in DNA assays, in which radioactive probes were used to map and identify DNA sequences, ... December 2012). "The methylomes of six bacteria". Nucleic Acids Research. 40 (22): 11450-62. doi:10.1093/nar/gks891. PMC ... Nucleic Acids Research. 28 (8): 32e-0. doi:10.1093/nar/28.8.e32. PMC 102836. PMID 10734209. Russell 2010, pp. 552-3. van der ...

https://en.wikipedia.org/wiki/Epigenomics

A.C.Wilson and N.O.Kaplan (1963) Enzymes and nucleic acids in systematics. Proceedings of the XVI International Congress of ... Two techniques, mtDNA and hybridization converge on a single answer, chimps as a species are most closely related to humans. ... Nucleic Acids Res. 15 (2): 529-42. doi:10.1093/nar/15.2.529. PMC 340450. PMID 2881260. Vigilant L, Stoneking M, Harpending H, ... Sibley CG, Ahlquist JE (1984). "The phylogeny of the hominoid primates, as indicated by DNA-DNA hybridization". J. Mol. Evol. ...

https://en.wikipedia.org/wiki/Molecular_anthropology

... with nucleic acids and proteins in silico. It allowed researchers to, for example, plan a DNA cloning experiment on the ... sequencing or hybridisation experiments plan cloning and run gels in silico align multiple protein or DNA sequences search ...

https://en.wikipedia.org/wiki/Vector_NTI

Nucleic Acids Research, 34 (4): 1166-73, doi:10.1093/nar/gkj517, PMC 1380255, PMID 16493140 Rota, Paul A.; Oberste, M. Steven; ... and pioneering virus discovery using gene hybridization array and DNA sequencing technologies. DeRisi uses microarrays ...

https://en.wikipedia.org/wiki/Joseph_DeRisi

Nucleic Acids Res. England. 39 (Database issue): D883-8. doi:10.1093/nar/gkq1101. PMC 3013693. PMID 21051338. http://tiara.gmi. ... information obtained from next generation sequencing techniques and ultra-high-resolution comparative genomic hybridization. ...

https://en.wikipedia.org/wiki/TIARA_(database)

Nucleic Acids Research. 16 (7): 3109. doi:10.1093/nar/16.7.3109. PMC 336462. PMID 3368322. MacLeod AR, Houlker C, Reinach FC, ... to band 9p13 by fluorescence in situ hybridisation". Cytogenetics and Cell Genetics. 71 (1): 94-5. doi:10.1159/000134070. PMID ... β-tropomyosin is roughly 32 kDa in molecular weight (284 amino acids), but multiple splice variants exist. Tropomysin is a ... which is one amino acid away from the C-terminus. β-tropomyosin also has a Serine residue at position 283, thus, it is likely ...

https://en.wikipedia.org/wiki/TPM2

McCormick-Graham M, Romero DP (April 1995). "Ciliate telomerase RNA structural features". Nucleic Acids Research. 23 (7): 1091- ... to chromosome 3 by fluorescence in situ hybridization and mouse chromosome painting". Genomics. 41 (2): 293-4. doi:10.1006/geno ... Nucleic Acids Research. 30 (2): 559-68. doi:10.1093/nar/30.2.559. PMC 99832. PMID 11788719. Zhang RG, Zhang RP, Wang XW, Xie H ... Nucleic Acids Research. 24 (3): 532-3. doi:10.1093/nar/24.3.532. PMC 145649. PMID 8602368. Soder AI, Hoare SF, Muire S, Balmain ...

https://en.wikipedia.org/wiki/Telomerase_RNA_component

Nucleic Acids Research. 41 (D1): D142-D151. doi:10.1093/nar/gks1041. PMC 3531201. PMID 23143107. Kim, P.; Yoon, S.; Kim, N.; ... Using Southern blotting and fluorescence in situ hybridization (FISH) on the genome, the researchers found no evidence of DNA ... Nucleic Acids Research. 38 (Database): D81-D85. doi:10.1093/nar/gkp982. PMC 2808913. PMID 19906715. Kim, Dae-Soo; Huh, Jae-Won ... Nucleic Acids Research. 40 (16): e123. doi:10.1093/nar/gks394. PMC 3439881. PMID 22570408. Iyer, M. K.; Chinnaiyan, A. M.; ...

https://en.wikipedia.org/wiki/Chimeric_RNA

... and nucleic acid hybridization has allowed for rapid and inexpensive detection of known viroids in biosecurity inspections, ... Although viroids are composed of nucleic acid, they do not code for any protein. The viroid's replication mechanism uses RNA ... Adkar-Purushothama CR, Perreault JP (August 2020). "Impact of Nucleic Acid Sequencing on Viroid Biology". International Journal ...

https://en.wikipedia.org/wiki/Viroid

Nucleic Acids Research (Epub ahead of print)Bargaje R, Alam MP, Patowary A, et al. (October 2012). "Proximity of H2A.Z ... "Chromosomal mapping of the human histone gene H2AZ to 4q24 by fluorescence in situ hybridization". Genomics (Submitted ... Nucleic Acids Res. 16 (3): 1113-24. doi:10.1093/nar/16.3.1113. PMC 334740. PMID 3344202. Kato S, Sekine S, Oh SW, et al. (1995 ... Nucleic Acids Research. 40 (18): 8965-78. doi:10.1093/nar/gks665. PMC 3467062. PMID 22821566. Rege M (2015). "Chromatin ...

https://en.wikipedia.org/wiki/Histone_H2A.Z

January 2015). "STRING v10: protein-protein interaction networks, integrated over the tree of life". Nucleic Acids Research. 43 ... February 2011). "High-resolution comparative genomic hybridization of inflammatory breast cancer and identification of ... DUF4509 is located at amino acid 45 to amino acid 228. In this domain of unknown function there is a conserved WLL sequence ... DUF4510 is located at amino acid 263 to amino acid 425. In this domain of unknown function there are two conserved sequence ...

https://en.wikipedia.org/wiki/C14orf80

2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415-8. doi:10.1093/nar/gkj139. PMC 1347501. PMID ... fusion gene in the blood and/or bone marrow cells of sufferers by cytogenic analysis using Fluorescence in situ hybridization ... The fusion gene may produce a protein consisting of the first 233 amino acids of FIP1L1 joined to the last 523 amino acids of ... RARA, the Retinoic acid receptor alpha gene, is located on human chromosome 17 at position q21.2 (i.e. 17q21.2), consists of 17 ...

https://en.wikipedia.org/wiki/FIP1L1

Nucleic Acid Hybridization ✖ Remove constraint Subject: Nucleic Acid Hybridization Subject RNA ✖ Remove constraint Subject: RNA ...

https://profiles.nlm.nih.gov/spotlight/pd/catalog?f%5Breadonly_format_ssim%5D%5B%5D=Text&f%5Breadonly_language_ssim%5D%5B%5D=English&f%5Breadonly_subject_ssim%5D%5B%5D=Nucleic+Acid+Hybridization&f%5Breadonly_subject_ssim%5D%5B%5D=RNA&per_page=20&search_field=all_fields&sort=readonly_title_ssim+asc&view=slideshow

Dabpna: Design, Synthesis And Nucleic Acids Binding Studies (430 views). Nucleosides Nucleotides Nucleic Acids (ISSN: 1525-7770 ... Gangamani, B.P., Kumar, V.A., Ganesh, K.N., Chiral analogues of peptide nucleic acids: Synthesis of 4-aminoprolyl nucleic acids ... Peptide nucleic acids (PNAs) containing thymine monomers derived from chiral amino acids: Hybridization and solubility ... Chiral analogues of peptide nucleic acids: Synthesis of 4-aminoprolyl nucleic acids and DNA complementation studies using UV/CD ...

https://www.ibb.cnr.it/?command=search&table=bibliography&idb=50106

Nucleic acid hybridization/probe tests. Two nucleic acid hybridization assays are FDA-cleared to detect C. trachomatis or N. ... gonorrhoeae nucleic acid have better performance characteristics than the Gonostat test. The gonorrhea nucleic acid genetic ... Nucleic acid amplification tests (NAATs) are the recommended test method.. *A self- or clinician-collected vaginal swab is the ... Nucleic acid amplification tests (NAATs) are the recommended test method.. *A first catch urine is the recommended sample type ...

https://www.cdc.gov/std/laboratory/2014labrec/recommendations2.htm

Visualization of the mycelia of wood-rotting fungi by fluorescence in situ hybridization using a peptide nucleic acid probe.. ... were visualized by fluorescence in situ hybridization (FISH) using a peptide nucleic acid (PNA) probe. Mycelia grown on wood ...

https://pesquisa.bvsalud.org/perinatal/resource/es/mdl-23391931

Mfold web server for nucleic acid folding and hybridization prediction.. Nucleic Acids Res. 2003; 31: 3406-3415. View in ... Mfold web server for nucleic acid folding and hybridization prediction.. Nucleic Acids Res. 2003; 31: 3406-3415 ... Thermodynamic analysis of nucleic acids and proteins in purified form and in cellular extracts.. Biophys. Chem. 1987; 26: 235- ... Nucleic Acid Res. Mol. Biol. 1983; 28: 101-142. View in Article *Scopus (224) ...

https://www.cell.com/molecular-cell/fulltext/S1097-2765

Nucleic Acid Hybridization Tests (DNA Probe). Nucleic acid hybridization methods can be used for the diagnosis of chlamydial ... The technical requirements and the necessary expertise to perform nucleic acid hybridization tests are similar to those of the ... nucleic acid hybridization tests, and rapid (stat) tests. Because nonculture tests do not require strict handling of specimens ... Perform a second nonculture test that identifies a C. trachomatis antigen or a nucleic acid sequence that is different from ...

https://wonder.cdc.gov/wonder/prevguid/p0000222/p0000222.asp

Detection of the rRNA amplification product sequences is achieved using nucleic acid hybridization (HPA). A single-stranded ... During the hybridization step, the sequence specific regions of the capture oligomers bind to specific regions of the target ... This temperature reduction allows hybridization to occur between the deoxyadenosine region on the capture oligomer and the poly ... of complementary oligonucleotide primers to specifically anneal and allow enzymatic amplification of the target nucleic acid ...

https://wwwn.cdc.gov/Nchs/Nhanes/limited_access/TRIC_J_R.htm

In this review, we will summarise the latest developments in peptide-based cellular delivery of nucleic acid cargos. We will ... CPPs are capable of mediating the cellular uptake of hydrophilic macromolecules like peptides and nucleic acids (e.g. siRNAs, ... Despite the fact that non-viral nucleic acid delivery systems are generally considered to be less efficient than viral vectors ... latter represent an assortment of fairly unrelated sequences essentially characterised by a high content of basic amino acids ...

https://www.mdpi.com/1422-0067/9/7/1276

Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplification. Nucleic Acids Res. 30 ... Comparative genomic hybridization using oligonucleotide microarrays and total genomic DNA. Proc. Natl Acad. Sci. USA 101, 17765 ... High resolution analysis of DNA copy number variation using comparative genomic hybridization to microarrays. Nature Genet. 20 ... Fluorescence in situ hybridization. A technique in which fluorescently labelled DNA probes are hybridized to interphase cells, ...

https://www.nature.com/articles/nrg1767?error=cookies_not_supported&code=b5905365-fca5-4932-875c-41c8de208e8e

Application of locked nucleic acid-based probes in fluorescence in situ hybridization. Applied Microbiology and Biotechnology, ... Mismatch discrimination in fluorescent in situ hybridization using different types of nucleic acids (Applied Microbiology and ... Fontenete, S., Leite, M., Guimarães, N., Madureira, P., et al. (2015). Towards fluorescence in vivo hybridization (FIVH) ...

https://www.ibmc.up.pt/research/publications?page=1&s=author&f%5Bag%5D=F

Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA ...

https://www.jove.com/v/52195/high-throughput-fluorometric-technique-for-assessment-macrophage?language=Norwegian

... the peptide nucleic acid-locked nucleic acid PCR clamp. Cancer Res. 65:7276-7282. 2005. View Article : Google Scholar : PubMed/ ... Ikeda S, Kubota T, Yuki M and Okamoto A: Exciton-controlled hybridization-sensitive fluorescent probes: Multicolor detection of ... Ludyga N, Grünwald B, Azimzadeh O, Englert S, Höfler H, Tapio S and Aubele M: Nucleic acids from long-term preserved FFPE ... Usefulness of peptide nucleic acid (PNA)-clamp smart amplification process version 2 (SmartAmp2) for clinical diagnosis of KRAS ...

https://www.spandidos-publications.com/10.3892/or.2015.3883/abstract

Peptide Nucleic Acid-Fluorescent in Situ Hybridization, Syndromic Panel-Based Testing, Microarrays}, Flow Cytometry, ... Acid-Fluorescent in Situ Hybridization, Syndromic Panel-Based Testing, Microarrays}, Flow Cytometry, Microfluidics, Immunoassay ... Acids, Acetic Acid, Phosphate, Histidine, Others), By Buffer Preparation (Liquid, Powder), By Material Form (Dry, Liquid), By ... Nucleic Acid Applications and Proteomics Applications), by End User (Research Centers, Pharmaceutical and Biotechnology ...

https://www.marketresearch.com/seek/Biotechnology-Oceania/57/1356/1.html

BVD-PI (Persistent Infection): All animals must have a negative Antigen Detection test (ear notch, nucleic acid hybridization, ...

https://www.usjersey.com/AJCA-NAJ-JMS/JMS/JMSHealthRequirements/DairylandProteinSaleHealthLetter.aspx

... dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR ... Deoxyribonucleic acid relatedness between Bacillus anthracis, Bacillus cereus and Bacillus thuringiensis. Microbiol Immunol. ...

https://wwwnc.cdc.gov/eid/article/8/10/02-0393-t1

Detecting Nucleic Acids through Hybridization and Amplification Assays. Commentary: J Appl Bioinforma Comput Biol. DOI: Robert ... Analysis of Amino Acid Decarboxylases Using Bioinformatics. Perspective: J Appl Bioinforma Comput Biol. DOI: David Bordes *Full ... Sequence Analysis of Holins by Reduced Amino Acid Alphabet Model and Permutation Approaches. Research Article: J Appl ...

https://www.scitechnol.com/editor-profile/Daniel_Peterson/

... revealing the presence of nucleic acids from anelloviruses, adenovirus-associated virus and 12 known respiratory viral ... Zuker M. 2003; Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 31:3406-3415 [View ... revealing the presence of nucleic acids from anelloviruses, adenovirus-associated virus and 12 known respiratory viral ...

https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.061143-0

"Interfacial Nucleic. Acid Hybridization Studied by Random Primer 32P Labeling. and Liquid-Phase Acoustic Network Analysis.", 66 ... and Hybridization of DNA Studied by the Use of Quartz. Crystal Oscillators." Anal. Chem., 65, 1925－1927.. [61] Su, H., K,M. R ... A Coated Piezoelectric Crystal Sensor for Acetic-Acid Vapor. Determination." Talanta, 40, 1725-1729.. [54] Okahata ,Y., H, ...

https://ndltd.ncl.edu.tw/cgi-bin/gs32/gsweb.cgi/login?o=dnclcdr&s=id=%22102TTU05106011%22.&searchmode=basic

S. Doughan, M.O. Noor, Y. Han and U.J. Krull, Resonance Energy Transfer-Based Nucleic Acid Hybridization Assays on Paper-Based ... F. Zhou, M.O. Noor and U.J. Krull, A paper-based sandwich format hybridization assay for unlabeled nucleic acid detection using ... S. Doughan, U. Uddayasankar and U.J. Krull, A paper-based resonance energy transfer nucleic acid hybridization assay using ... F508 gene mutation using a paper-based nucleic acid hybridization assay and a smartphone camera, The Analyst, 143: 3049-3058, ...

https://www.utm.utoronto.ca/cps/people/ulrich-j-krull

The Genomics Core in the CRI will offer nucleic acid preparation, sequencing, real-time PCR and microarray hybridisation ...

https://openwetware.org/wiki/CRI_Genomics_Core

Hybridization Incubators Market 2020 by Manufacturers, Regions, Type and Application, Regional Forecast to 2025 ... Nucleic Acid Hybridizations. Molecular Biology Assays. Other. The in-depth report also focuses on various segments, such as ... Hybridization Incubators Market (Updated Version Available) Hybridization Incubators Market 2020 by Manufacturers, Regions, ... In Market segmentation by types of Hybridization Incubators, the report covers-. Bench-top Type. Floor-standing Type. Other. In ...

https://www.reportsinsights.com/industry-forecast/hybridization-incubators-market-growth-3962

Sustainable antibacterial hydrogel from hybridization mediated self-assembly of DNA Quantum Dot and genomic DNA extracted from ... physical chemistry and biophysics of nucleic acids, new structures and new technologies involving nucleic acid modelling and ... Point-of-care nucleic acid tests: assays and devices Ofer I. Wilner, Doron Yesodi and Yossi Weizmann ... Stimuli-responsive nucleic acid nanostructures for efficient drug delivery Changping Yang, Xiaohui Wu, Jianbing Liu and Baoquan ...

https://pubs.rsc.org/en/journals/articlecollectionlanding?sercode=nr&themeid=7fc996e0-a9f4-4f22-807e-edb991f88db0

Peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay for specific detection of mycobacterium immunogenum ... and acids during metal conduit manufacturing, Republic Conduit, Louisville, Kentucky. ...

http://www2a.cdc.gov/nioshtic-2/BuildQyr.asp?s1=metalworking&t3=1&f8=KW&f1=TI&s5=metalworking&t7=1&Startyear=&t1=1&f6=KW&s2=%27metal+working%27&t4=1&f9=%2A&terms=12&Adv=1&ct=&B1=Search&f2=TI&s6=%27metal+working%27&t8=0&t2=1&f7=KW&Limit=500&Sort=DP+DESC&s3=cutting&t5=1&f3=TI&s7=cutting&s4=machining&t6=1&EndYear=&f4=TI&s8=machining&f5=KW&s9=%27oil%2A%27+or+%27fluid%2A%27+or+%27coolant%2A%27+or+%27liquid%2A%27&D1=10&PageNo=104&RecNo=104&View=b&

... specific nucleic acid sequences, peptides, proteins or metal ions. One of the main advantages of using nucleic acids as ... The review reports on the function mode of nucleic acid biosensors and introduces recent developments and applications in the ... Nucleic acids are well suited to serve as biosensors for the fast and reliable detection of small organic molecules, such as a ... dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridisation. PCR ...

https://digital-library.theiet.org/content/journals/10.1049/ip-nbt_20050047

Acid Hybridization, Nucleic Acid Hybridizations, Nucleic Hybridization, Nucleic Acid Hybridizations, Nucleic Acid Nucleic Acid ... Hybridization, Nucleic Acid. Hybridizations, Genomic. Hybridizations, Nucleic Acid. Nucleic Acid Hybridizations. Tree number(s ... Acid Hybridization, Nucleic. Acid Hybridizations, Nucleic. Genomic Hybridization. Genomic Hybridizations. Hybridization, ... Nucleic Acid Hybridization - Preferred Concept UI. M0015052. Scope note. Widely used technique which exploits the ability of ...

https://decs.bvsalud.org/en/ths/resource/?id=9880&filter=ths_exact_term&q=HIBRIDACI%C3%93N+DE+%C3%81CIDO+NUCLEICO

Nucleic Acid Based Assay Involving A Hybridization Step With A Nucleic Acid Probe, Involving A Single Nucleotide Polymorphism ( ... hybridization of a nucleic acid probe to a nucleic acid target, under specified conditions. Methods for conducting ... The term "high stringency hybridization conditions," when used in connection with nucleic acid hybridization, means those ... wherein said determining comprises isolating nucleic acid from said sample and analyzing said nucleic acid to determine the ...

https://patents.justia.com/patent/20130079423

Nucleic acid amplification tests (NAATs) are the recommended test method. (cdc.gov)
Target amplification assays are based on the ability of complementary oligonucleotide primers to specifically anneal and allow enzymatic amplification of the target nucleic acid strands. (cdc.gov)
Detection of the rRNA amplification product sequences is achieved using nucleic acid hybridization (HPA). (cdc.gov)
This test, using Hybrid Capture 2 technology, is a nucleic acid hybridization microplate assay with signal amplification. (cdc.gov)
After amplification the samples are typed by hybridization to the typing strips followed by colorimetric detection. (cdc.gov)
All animals must have a negative Antigen Detection test (ear notch, nucleic acid hybridization, polymerase chain reaction amplification (PCR), viral isolation or Elisa). (usjersey.com)
Nucleic acid sequence-based amplification (NASBA) was employed with a set of universal primers and probe based on the 3' non-coding region of the dengue viral RNA sequence. (who.int)
A person with laboratory isolation of typical gram-negative, oxidase-positive diplococcic by culture (presumptive Neisseria gonorrhoeae ) from a clinical specimen, or demonstration of N. gonorrhoeae in a clinical specimen by detection of antigen or detection of nucleic acid via nucleic acid amplification (e.g. (cdc.gov)

Visualization of the mycelia of wood-rotting fungi by fluorescence in situ hybridization using a peptide nucleic acid probe. (bvsalud.org)
White rot fungus , Phanerochaete chrysosporium , and brown rot fungus , Postia placenta , grown on agar plates, were visualized by fluorescence in situ hybridization (FISH) using a peptide nucleic acid (PNA) probe. (bvsalud.org)
2015). Erratum to: Mismatch discrimination in fluorescent in situ hybridization using different types of nucleic acids (Applied Microbiology and Biotechnology, 10.1007/s00253-015-6389-4) . (up.pt)
2016). Application of locked nucleic acid-based probes in fluorescence in situ hybridization . (up.pt)
Peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay for specific detection of mycobacterium immunogenum and DNA-FISH assay for analysis of pseudomonads in metalworking fluid s and sputum. (cdc.gov)

S. Doughan, M.O. Noor, Y. Han and U.J. Krull , Resonance Energy Transfer-Based Nucleic Acid Hybridization Assays on Paper-Based Platforms Using Emissive Nanoparticles as Donors, Methods in Molecular Biology , 1571: 301-326, 2017 (invited). (utoronto.ca)

Reaction To Detect hybridizations or polymerase chain reaction (PCR), apply a single detection method to a diversity of organisms. (cdc.gov)
2015). Towards fluorescence in vivo hybridization (FIVH) detection of H. pylori in gastric mucosa using advanced LNA probes . (up.pt)
A. Shahmuradyan, M. Moazami-Goudarzi, F. Litazume, G.S. Espie and U.J. Krull , Paper-based platform for detection by hybridization using intrinsically labeled fluorescent oligonucleotide probes on quantum dots, The Analyst , 144: 1223-1229, 2019. (utoronto.ca)
K. Malhotra, M. Omair Noor and U.J. Krull , Detection of Cystic Fibrosis transmembrane conductance regulator ∆F508 gene mutation using a paper-based nucleic acid hybridization assay and a smartphone camera, The Analyst , 143: 3049-3058, 2018. (utoronto.ca)
Nucleic acids are well suited to serve as biosensors for the fast and reliable detection of small organic molecules, such as a number of metabolites or antibiotics, specific nucleic acid sequences, peptides, proteins or metal ions. (theiet.org)

Gangamani, B.P., Kumar, V.A., Ganesh, K.N., Chiral analogues of peptide nucleic acids: Synthesis of 4-aminoprolyl nucleic acids and DNA complementation studies using UV/CD spectroscopy (1999) Tetrahedron, 55 (1), pp. 177-192. (cnr.it)
Nelson, K.E., Levy, M., Miller, S.L., Peptide nucleic acids rather than RNA may have been the first genetic molecule (2000) Proceedings of the National Academy of Sciences of the United States of America, 97 (8), pp. 3868-3871. (cnr.it)
Peptide nucleic acids have high biological stability and higher affinity for complementary DNA or RNA sequences than analogous DNA oligomers. (lookformedical.com)

Surface plasmon field-enhanced fluorescence spectroscopy in PCR product analysis by peptide nucleic acid probes. (mpg.de)

CPPs are capable of mediating the cellular uptake of hydrophilic macromolecules like peptides and nucleic acids (e.g. siRNAs, aptamers and antisense-oligonucleotides), which are internalised by cells at a very low rate when applied alone. (mdpi.com)
Selectivity can be established using immobilized biomolecules on nanoparticles for interaction with proteins, peptides and nucleic acid sequences. (utoronto.ca)

A. Shahmuradyan and U.J. Krull , Intrinsically Labeled Fluorescent Oligonucleotide Probes on Quantum Dots for Transduction of Nucleic Acid Hybridization, Analytical Chemistry , 88, 6: 3186-3193, 2016. (utoronto.ca)

The latter represent an assortment of fairly unrelated sequences essentially characterised by a high content of basic amino acids and a length of 10-30 residues. (mdpi.com)
Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. (bvsalud.org)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)
Antisense ácido nucleico de fita única que se ligam via hibridização oligonucleotides are short sequences of single-stranded de Watson-Crick no seu RNA complementar, levando à nucleic acids which bind via Watson-Crick hybridization inibição da síntese da proteína que seria codificada por essa to its complementary RNA, leading to protein synthesis região do RNA. (bvsalud.org)

Surface density dependence of PCR amplicon hybridization on PNA/DNA probe layers. (mpg.de)
Polymerase Chain Reaction [PCR]) or hybridization with a nucleic acid probe. (cdc.gov)
Any specific DNA present on the blot will combine labeled DNA to neutral dimethylsulphate acid, with the labeled probe and is detected auto hydrazine NaCl piperidine or hydrazine piperidine. (who.int)

Diverse Loci cation of nucleic acid amplifications requires selecting appro- priate oligonucleotide primers and optimizing conditions to Associated with maximize sensitivity and specificity. (cdc.gov)
Oligonucleotide hybridization studied by a surface plasmon diffraction sensor (SPDS). (mpg.de)

In this minireview, we summarized the recent progress of controllable reconfigurations of complex DNA nanostructures induced by nucleic acid strands, environmental stimuli and enzymatic treatments. (rsc.org)

Health hazard evaluation report: HETA-2006-0332-3058, evaluation of worker exposures to noise, metalworking fluid s, welding fumes, and acids during metal conduit manufacturing, Republic Conduit, Louisville, Kentucky. (cdc.gov)

The review reports on the function mode of nucleic acid biosensors and introduces recent developments and applications in the field. (theiet.org)

During the hybridization step, the sequence specific regions of the capture oligomers bind to specific regions of the target molecules. (cdc.gov)

Despite the fact that non-viral nucleic acid delivery systems are generally considered to be less efficient than viral vectors, they have gained much interest in recent years due to their superior safety profile compared to their viral counterpart. (mdpi.com)
Nasopharyngeal aspirates (NPAs) from 120 Chilean infants from Santiago with acute lower respiratory tract infections were analysed by viral metagenomics, revealing the presence of nucleic acids from anelloviruses, adenovirus-associated virus and 12 known respiratory viral pathogens. (microbiologyresearch.org)

This temperature reduction allows hybridization to occur between the deoxyadenosine region on the capture oligomer and the poly-deoxythymidine molecules that are covalently attached to the magnetic particles. (cdc.gov)
The order of amino acids as they occur in a polypeptide chain. (lookformedical.com)

One of the main advantages of using nucleic acids as biosensors is that they can be modulated to respond allosterically to specific effectors. (theiet.org)

used for hybridization studies in order to identify microorganisms and for genetic studies. (lookformedical.com)

The Genomics Core in the CRI will offer nucleic acid preparation, sequencing, real-time PCR and microarray hybridisation services for CRI researchers. (openwetware.org)

The results showed that those 2 isolates were multi-drug resistant and the DNA sequencing analysis showed that the alignment of nucleic acid of DNA in isolates of mycobacteria other than M. tuberculosis was different from that of M. tuberculosis complex. (who.int)

S. Doughan, U. Uddayasankar, A. Peri and U.J. Krull , A Paper-Based Multiplexed Resonance Energy Transfer Nucleic Acid Hybridization Assay Using a Single Form of Upconversion Nanoparticle as Donor and Quantum Dots as Acceptors, Analytica Chimica Acta , 962: 88-96, 2017. (utoronto.ca)

This minireview summarizes the recent advances in stimuli-responsive nucleic acid nanostructures for efficient drug delivery in response to endogenous and exogenous stimuli (redox gradient, pH, nuclease, biomacromolecule, and light). (rsc.org)

DNA computing has emerged as a promising alternative to achieve programmable behaviors in chemistry by repurposing the nucleic acid molecules into chemical hardware upon which synthetic chemical programs can be executed. (rsc.org)
Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. (lookformedical.com)

In some embodiments determining the amount of LOH in a sample comprises isolating nucleic acids from the sample and analyzing the nucleic acid to determine the amount of LOH. (justia.com)

The latest Market report on the Hybridization Incubators Market highlights detailed study on the emerging trends, drivers, and opportunities for the forecast period 2020 - 2025. (reportsinsights.com)
What is the estimated growth rate and Market share and size of the Hybridization Incubators Market for the forecast period 2020 - 2025? (reportsinsights.com)
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Anatomy1

Organisms4

Chemicals and Drugs22

Analytical, Diagnostic and Therapeutic Techniques and Equipment14

Phenomena and Processes15

Information Science4

Health Care3

Identification and characterization of the human orthologue of yeast Pex14p. (1/18626)

The LIM-only protein PINCH directly interacts with integrin-linked kinase and is recruited to integrin-rich sites in spreading cells. (2/18626)

Isolation of human transcripts expressed in hamster cells from YACs by cDNA representational difference analysis. (3/18626)

Single atom modification (O-->S) of tRNA confers ribosome binding. (4/18626)

Suppression subtractive hybridization identifies high glucose levels as a stimulus for expression of connective tissue growth factor and other genes in human mesangial cells. (5/18626)

Human geranylgeranyl diphosphate synthase. cDNA cloning and expression. (6/18626)

The biosynthesis of transfer RNA in insects. II. Isolation of transfer RNA precursors from the posterior silk gland of Bombyx mori. (7/18626)

Burkholderia cocovenenans (van Damme et al. 1960) Gillis et al. 1995 and Burkholderia vandii Urakami et al. 1994 are junior synonyms of Burkholderia gladioli (Severini 1913) Yabuuchi et al. 1993 and Burkholderia plantarii (Azegami et al. 1987) Urakami et al. 1994, respectively. (8/18626)

Nucleic Acid Hybridization

DNA Probes

DNA, Viral

RNA, Viral

DNA

Base Sequence

Nucleic Acid Denaturation

Gammaretrovirus

DNA, Bacterial

Nucleic Acids

Peptide Nucleic Acids

Oligonucleotide Probes

Biotin

Methods

RNA, Bacterial

Indicators and Reagents

In Situ Hybridization

Retroviridae

Fluorescent Dyes

Tritium

Molecular Sequence Data

In Situ Hybridization, Fluorescence

Cloning, Molecular

Polymerase Chain Reaction

Genes, Viral

RNA-Directed DNA Polymerase

DNA Restriction Enzymes

Species Specificity

Antigens, Viral

Hybridization, Genetic

Micropore Filters

Nucleic Acid Conformation

Cell Line

RNA

Nucleic Acid Probes

RNA, Messenger

Nucleic Acid Amplification Techniques

Escherichia coli

Centrifugation, Density Gradient

Plasmids

Sensitivity and Specificity

Transcription, Genetic

Oligonucleotides

Hot Temperature

Temperature

Kinetics

Herpesvirus 4, Human

Comparative Genomic Hybridization

RNA Probes

Amino Acid Sequence

Sequence Analysis, DNA

Phylogeny

RNA, Ribosomal, 16S

Identification and characterization of the human orthologue of yeast Pex14p. (1/18626)

The LIM-only protein PINCH directly interacts with integrin-linked kinase and is recruited to integrin-rich sites in spreading cells. (2/18626)

Isolation of human transcripts expressed in hamster cells from YACs by cDNA representational difference analysis. (3/18626)

Single atom modification (O-->S) of tRNA confers ribosome binding. (4/18626)

Suppression subtractive hybridization identifies high glucose levels as a stimulus for expression of connective tissue growth factor and other genes in human mesangial cells. (5/18626)

Human geranylgeranyl diphosphate synthase. cDNA cloning and expression. (6/18626)

The biosynthesis of transfer RNA in insects. II. Isolation of transfer RNA precursors from the posterior silk gland of Bombyx mori. (7/18626)

Burkholderia cocovenenans (van Damme et al. 1960) Gillis et al. 1995 and Burkholderia vandii Urakami et al. 1994 are junior synonyms of Burkholderia gladioli (Severini 1913) Yabuuchi et al. 1993 and Burkholderia plantarii (Azegami et al. 1987) Urakami et al. 1994, respectively. (8/18626)

Nucleic acid hybridization

Hybridization assay

Grapevine virus A

Hybridization probe

Nucleic acid test

List of RNA structure prediction software

CCDC188

George Stark

C20orf27

Nucleic acid structure prediction

Transmembrane protein 217

C7orf25

Mycobacterium tuberculosis sRNA

TMEM211

Tex36

Basic leucine zipper and W2 domain-containing protein 2

SLC46A3

Ligation (molecular biology)

Suspension array technology