Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.
Deoxyribonucleic acid that makes up the genetic material of viruses.
Ribonucleic acid that makes up the genetic material of viruses.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible.
A genus of RETROVIRIDAE comprising endogenous sequences in mammals, related RETICULOENDOTHELIOSIS VIRUSES, AVIAN, and a reptilian virus. Many species contain oncogenes and cause leukemias and sarcomas.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
High molecular weight polymers containing a mixture of purine and pyrimidine nucleotides chained together by ribose or deoxyribose linkages.
DNA analogs containing neutral amide backbone linkages composed of aminoethyl glycine units instead of the usual phosphodiester linkage of deoxyribose groups. Peptide nucleic acids have high biological stability and higher affinity for complementary DNA or RNA sequences than analogous DNA oligomers.
Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.
A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.
A series of steps taken in order to conduct research.
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)
A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.
Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
The functional hereditary units of VIRUSES.
An enzyme that synthesizes DNA on an RNA template. It is encoded by the pol gene of retroviruses and by certain retrovirus-like elements. EC 2.7.7.49.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Substances elaborated by viruses that have antigenic activity.
The genetic process of crossbreeding between genetically dissimilar parents to produce a hybrid.
A membrane or barrier with micrometer sized pores used for separation purification processes.
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
Established cell cultures that have the potential to propagate indefinitely.
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Nucleic acid which complements a specific mRNA or DNA molecule, or fragment thereof; used for hybridization studies in order to identify microorganisms and for genetic studies.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
The rate dynamics in chemical or physical systems.
The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies.
A method for comparing two sets of chromosomal DNA by analyzing differences in the copy number and location of specific sequences. It is used to look for large sequence changes such as deletions, duplications, amplifications, or translocations.
RNA, usually prepared by transcription from cloned DNA, which complements a specific mRNA or DNA and is generally used for studies of virus genes, distribution of specific RNA in tissues and cells, integration of viral DNA into genomes, transcription, etc. Whereas DNA PROBES are preferred for use at a more macroscopic level for detection of the presence of DNA/RNA from specific species or subspecies, RNA probes are preferred for genetic studies. Conventional labels for the RNA probe include radioisotope labels 32P and 125I and the chemical label biotin. RNA probes may be further divided by category into plus-sense RNA probes, minus-sense RNA probes, and antisense RNA probes.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
The relationships of groups of organisms as reflected by their genetic makeup.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.

Identification and characterization of the human orthologue of yeast Pex14p. (1/18626)

Pex14p is a central component of the peroxisomal protein import machinery, which has been suggested to provide the point of convergence for PTS1- and PTS2-dependent protein import in yeast cells. Here we describe the identification of a human peroxisome-associated protein (HsPex14p) which shows significant similarity to the yeast Pex14p. HsPex14p is a carbonate-resistant peroxisomal membrane protein with its C terminus exposed to the cytosol. The N terminus of the protein is not accessible to exogenously added antibodies or protease and thus might protrude into the peroxisomal lumen. HsPex14p overexpression leads to the decoration of tubular structures and mislocalization of peroxisomal catalase to the cytosol. HsPex14p binds the cytosolic receptor for the peroxisomal targeting signal 1 (PTS1), a result consistent with a function as a membrane receptor in peroxisomal protein import. Homo-oligomerization of HsPex14p or interaction of the protein with the PTS2-receptor or HsPex13p was not observed. This distinguishes the human Pex14p from its counterpart in yeast cells and thus supports recent data suggesting that not all aspects of peroxisomal protein import are conserved between yeasts and humans. The role of HsPex14p in mammalian peroxisome biogenesis makes HsPEX14 a candidate PBD gene for being responsible for an unrecognized complementation group of human peroxisome biogenesis disorders.  (+info)

The LIM-only protein PINCH directly interacts with integrin-linked kinase and is recruited to integrin-rich sites in spreading cells. (2/18626)

PINCH is a widely expressed and evolutionarily conserved protein comprising primarily five LIM domains, which are cysteine-rich consensus sequences implicated in mediating protein-protein interactions. We report here that PINCH is a binding protein for integrin-linked kinase (ILK), an intracellular serine/threonine protein kinase that plays important roles in the cell adhesion, growth factor, and Wnt signaling pathways. The interaction between ILK and PINCH has been consistently observed under a variety of experimental conditions. They have interacted in yeast two-hybrid assays, in solution, and in solid-phase-based binding assays. Furthermore, ILK, but not vinculin or focal adhesion kinase, has been coisolated with PINCH from mammalian cells by immunoaffinity chromatography, indicating that PINCH and ILK associate with each other in vivo. The PINCH-ILK interaction is mediated by the N-terminal-most LIM domain (LIM1, residues 1 to 70) of PINCH and multiple ankyrin (ANK) repeats located within the N-terminal domain (residues 1 to 163) of ILK. Additionally, biochemical studies indicate that ILK, through the interaction with PINCH, is capable of forming a ternary complex with Nck-2, an SH2/SH3-containing adapter protein implicated in growth factor receptor kinase and small GTPase signaling pathways. Finally, we have found that PINCH is concentrated in peripheral ruffles of cells spreading on fibronectin and have detected clusters of PINCH that are colocalized with the alpha5beta1 integrins. These results demonstrate a specific protein recognition mechanism utilizing a specific LIM domain and multiple ANK repeats and suggest that PINCH functions as an adapter protein connecting ILK and the integrins with components of growth factor receptor kinase and small GTPase signaling pathways.  (+info)

Isolation of human transcripts expressed in hamster cells from YACs by cDNA representational difference analysis. (3/18626)

Gene isolation methods used during positional cloning rely on physical contigs consisting of bacterial artificial chromosomes, P1, or cosmid clones. However, in most instances, the initial framework for physical mapping consists of contigs of yeast artificial chromosome (YACs), large vectors that are suboptimal substrates for gene isolation. Here we report a strategy to identify gene sequences contained within a YAC by using cDNA representational difference analysis (RDA) to directly isolate transcripts expressed from the YAC in mammalian cells. The RDA tester cDNAs were generated from a previously reported hamster cell line derived by stable transfer of a 590-kb YAC (911D5) that expressed NPC1, the human gene responsible for Niemann-Pick type C (NP-C). The driver cDNAs were generated from a control hamster cell line that did not contain the YAC that expressed NPC1. Among the gene fragments obtained by RDA, NPC1 was the most abundant product. In addition, two non-NPC1 fragments were isolated that were mapped to and expressed from 911D5. One of these RDA gene fragments (7-R) spans more than one exon and has 98% sequence identity with a human cDNA clone reported previously as an expressed sequence tag (EST), but not mapped to a chromosomal region. The other fragment (2-R) that had no significant sequence similarities with known mammalian genes or ESTs, was further localized to the region of overlap between YACs 911D5 and 844E3. The latter YAC is part of a contig across the NP-C candidate region, but does not contain NPC1. This two-part approach in which stable YAC transfer is followed by cDNA RDA should be a useful adjunct strategy to expedite the cloning of human genes when a YAC contig is available across a candidate interval.  (+info)

Single atom modification (O-->S) of tRNA confers ribosome binding. (4/18626)

Escherichia coli tRNALysSUU, as well as human tRNALys3SUU, has 2-thiouridine derivatives at wobble position 34 (s2U*34). Unlike the native tRNALysSUU, the full-length, unmodified transcript of human tRNALys3UUU and the unmodified tRNALys3UUU anticodon stem/loop (ASLLys3UUU) did not bind AAA- or AAG-programmed ribosomes. In contrast, the completely unmodified yeast tRNAPhe anticodon stem/loop (ASLPheGAA) had an affinity (Kd = 136+/-49 nM) similar to that of native yeast tRNAPheGmAA (Kd = 103+/-19 nM). We have found that the single, site-specific substitution of s2U34 for U34 to produce the modified ASLLysSUU was sufficient to restore ribosomal binding. The modified ASLLysSUU bound the ribosome with an affinity (Kd = 176+/-62 nM) comparable to that of native tRNALysSUU (Kd = 70+/-7 nM). Furthermore, in binding to the ribosome, the modified ASLLys3SUU produced the same 16S P-site tRNA footprint as did native E. coli tRNALysSUU, yeast tRNAPheGmAA, and the unmodified ASLPheGAA. The unmodified ASLLys3UUU had no footprint at all. Investigations of thermal stability and structure monitored by UV spectroscopy and NMR showed that the dynamic conformation of the loop of modified ASLLys3SUU was different from that of the unmodified ASLLysUUU, whereas the stems were isomorphous. Based on these and other data, we conclude that s2U34 in tRNALysSUU and in other s2U34-containing tRNAs is critical for generating an anticodon conformation that leads to effective codon interaction in all organisms. This is the first example of a single atom substitution (U34-->s2U34) that confers the property of ribosomal binding on an otherwise inactive tRNA.  (+info)

Suppression subtractive hybridization identifies high glucose levels as a stimulus for expression of connective tissue growth factor and other genes in human mesangial cells. (5/18626)

Accumulation of mesangial matrix is a pivotal event in the pathophysiology of diabetic nephropathy. The molecular triggers for matrix production are still being defined. Here, suppression subtractive hybridization identified 15 genes differentially induced when primary human mesangial cells are exposed to high glucose (30 mM versus 5 mM) in vitro. These genes included (a) known regulators of mesangial cell activation in diabetic nephropathy (fibronectin, caldesmon, thrombospondin, and plasminogen activator inhibitor-1), (b) novel genes, and (c) known genes whose induction by high glucose has not been reported. Prominent among the latter were genes encoding cytoskeleton-associated proteins and connective tissue growth factor (CTGF), a modulator of fibroblast matrix production. In parallel experiments, elevated CTGF mRNA levels were demonstrated in glomeruli of rats with streptozotocin-induced diabetic nephropathy. Mannitol provoked less mesangial cell CTGF expression in vitro than high glucose, excluding hyperosmolality as the key stimulus. The addition of recombinant CTGF to cultured mesangial cells enhanced expression of extracellular matrix proteins. High glucose stimulated expression of transforming growth factor beta1 (TGF-beta1), and addition of TGF-beta1 to mesangial cells triggered CTGF expression. CTGF expression induced by high glucose was partially suppressed by anti-TGF-beta1 antibody and by the protein kinase C inhibitor GF 109203X. Together, these data suggest that 1) high glucose stimulates mesangial CTGF expression by TGFbeta1-dependent and protein kinase C dependent pathways, and 2) CTGF may be a mediator of TGFbeta1-driven matrix production within a diabetic milieu.  (+info)

Human geranylgeranyl diphosphate synthase. cDNA cloning and expression. (6/18626)

Geranylgeranyl diphosphate (GGPP) synthase (GGPPSase) catalyzes the synthesis of GGPP, which is an important molecule responsible for the C20-prenylated protein biosynthesis and for the regulation of a nuclear hormone receptor (LXR.RXR). The human GGPPSase cDNA encodes a protein of 300 amino acids which shows 16% sequence identity with the known human farnesyl diphosphate (FPP) synthase (FPPSase). The GGPPSase expressed in Escherichia coli catalyzes the GGPP formation (240 nmol/min/mg) from FPP and isopentenyl diphosphate. The human GGPPSase behaves as an oligomeric molecule with 280 kDa on a gel filtration column and cross-reacts with an antibody directed against bovine brain GGPPSase, which differs immunochemically from bovine brain FPPSase. Northern blot analysis indicates the presence of two forms of the mRNA.  (+info)

The biosynthesis of transfer RNA in insects. II. Isolation of transfer RNA precursors from the posterior silk gland of Bombyx mori. (7/18626)

The occurrence of precursors to tRNA in the post-polysomal fraction of the posterior silk gland of Bombyx mori was demonstrated by pulse-chase labeling and DNA-RNA hybridization competition experiments. These precursors had molecular sizes ranging from 4S to 5S on polyacrylamide gel electrophoresis. Analysis of the incorporation of the methyl group from [methyl-14C]methionine revealed that a radioactive peak on polyacrylamide gel appeared in the 4.5S region during brief labeling. This suggested that some methylation occurred at the 4.5S precursor step.  (+info)

Burkholderia cocovenenans (van Damme et al. 1960) Gillis et al. 1995 and Burkholderia vandii Urakami et al. 1994 are junior synonyms of Burkholderia gladioli (Severini 1913) Yabuuchi et al. 1993 and Burkholderia plantarii (Azegami et al. 1987) Urakami et al. 1994, respectively. (8/18626)

Reference strains of Burkholderia cocovenenans and Burkholderia vandii were compared with strains of other Burkholderia species using SDS-PAGE of whole-cell proteins, DNA-DNA hybridization and extensive biochemical characterization. Burkholderia gladioli and B. cocovenenans were indistinguishable in the chemotaxonomic and biochemical analyses. Burkholderia plantarii and B. vandii had indistinguishable whole-cell protein patterns but the B. vandii type strain differed from B. plantarii strains in several biochemical tests. The DNA-DNA binding levels (higher than 70%) indicated that (i) B. gladioli and B. cocovenenans, and (ii) B. plantarii and B. vandii each represent a single species. It is concluded that B. cocovenenans and B. vandii are junior synonyms of B. gladioli and B. plantarii, respectively.  (+info)

A nucleic acid hybridization assay employing an immobilized or immobilizable polynucleotide probe selected to form DNA.RNA or RNA.RNA hybrids with the particular polynucleotide sequence to be determined. Resulting hybrids are detected by binding of an antibody reagent, preferably labeled with a detectable chemical group, selective for binding the hybrids in the presence of the single stranded sample and probe nucleic acids. No immobilization or labeling of sample nucleic acids is necessary and hybridization can be performed entirely in solution.
You searched for: Exhibit Tags phage Remove constraint Exhibit Tags: phage Format Text Remove constraint Format: Text Subject Nucleic Acid Hybridization Remove constraint Subject: Nucleic Acid Hybridization Subject Simian virus 40 Remove constraint Subject: Simian virus 40 ...
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Definition of Nucleic acid hybridization with photos and pictures, translations, sample usage, and additional links for more information.
Nucleic acid hybridization has been used in an attempt to determine the fraction of spleen DNA which is homologous to immunoglobulin messenger RNA (mRNA). Microsomes prepared from the mouse myeloma MOPC 104E and from spleens of mice hyperimmunized with Salmonella typhi were used as a source of RNA rich in immunoglobulin mRNA. It was shown that RNA extracted from microsomes contained a very limited number of RNA species and was essentially uncontaminated by other cytoplasmic and nuclear RNAs. Hyperimmune spleen DNA was hybridized with radiolabeled RNA from spleen and myeloma microsomes in the presence of unlabeled liver RNA. It was assumed that liver RNA should compete for the binding of most or all microsomal RNA species other than immunoglobulin mRNA. The amount of presumptive immunoglobulin mRNA bound indicated that spleen cells contain about 6 to 14 × 103 sequences per haploid genome for immunoglobulins of the size of the variable region of immunoglobulin polypeptide chains. The data favor ...
Background Optical imaging (OI) techniques such as bioluminescence and fluorescence imaging have been widely used to track diseases in a non-invasive manner within living subjects. These techniques generally require bioluminescent and fluorescent probes. Here we demonstrate the feasibility of using radioactive probes for in vivo molecular OI. Methodology/Principal Findings By taking the advantages of low energy window of light (1.2-3.1 eV, 400-1000 nm) resulting from radiation, radionuclides that emit charged particles such as β+ and β− can be successfully imaged with an OI instrument. In vivo optical images can be obtained for several radioactive probes including 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG), Na18F, Na131I, 90YCl3 and a 90Y labeled peptide that specifically target tumors. Conclusions/Significance These studies demonstrate generalizability of radioactive OI technique. It provides a new molecular imaging strategy and will likely have significant impact on both small animal and
Buy or Rent Chapter 07 - Nucleic Acid Hybridization: Principles and Applications (Human Molecular Genetics) as an eTextbook and get instant access.
Single-molecule measurements of DNA hybridization kinetics are mostly performed on a surface or inside a trap. Here we demonstrate a time-resolved, 3D single-molecule tracking (3D-SMT) method that allows us to follow a freely diffusing ssDNA molecule in solution for hundreds of milliseconds or even seconds a
Lesions in genetic sequences, for example, the sickle cell anemia mutation in the beta globin gene, are detected by means of interactive labels in a nucleic acid hybridization assay. A signal is gener
The Suppression Subtractive Hybridization (SSH) method has previously been used to generate tissue-specific cDNA libraries and to compare gene expression in different types of tissues. We propose that the SSH method can be modified to serve as a mutation detection method. This modified SSH method would scan the entire genome for sequence disruptions in DNA. Two plasmids have been chosen to serve as a model system. One plasmid is a PGL-2 plasmid, and the second plasmid is a PGL-2 plasmid that contains a 935 base pair (bp) segment of the G6PDH gene. The goal of this project is to detect the 935 bp insert in the PGL-2 plasmid. The purpose of the plasmid model system is to demonstrate that the modified SSH method is feasible, and successful as a mutation detection method.
complementary nucleotide sequences in a hybridization process. Tools in molecular biology relying on such a hybridization process include the polymerase chain reaction (PCR; and all methods based thereon), subtractive hybridisation, random primer extension, huclease S1 mapping, primer extension, reverse transcription, cDNA synthesis; differential display of RNAs, and DMA sequence determination, Northern blotting (RNA blotting), Southern blotting (DNA blotting). The hybridisation process can also occur with one of the complementary nucleic acids immobilised to a matrix such as magnetic beads, Sepharose beads or any other resin; Tools in molecular biology relying on such a process include the isolation of poly (A+) mRNA. The hybridisation process can furthermore occur with one of the complementary nucleic acids immobilised to a solid support such as a nitro -cellulose or nylon membrane or immobilised by e.g. photolithography to, for example, a siliceous glass support (the latter known as nucleic ...
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TY - JOUR. T1 - Novel differential gene expression in human cirrhosis detected by suppression subtractive hybridization. AU - Shackel, N. AU - McGuinness, Peter. AU - Abbott, Catherine. AU - Gorrell, M. AU - McCaughan, Geoffrey. PY - 2003. Y1 - 2003. M3 - Article. VL - 38. SP - 577. EP - 588. JO - Hepatology. JF - Hepatology. SN - 0270-9139. IS - 3. ER - ...
Nucleic acid fragments have been obtained from the genome of mycobacteria, and applications of the nucleic acid fragments in the diagnosis of mycobacterial infections are described. More particularly, the present invention concerns an isolated polynucleotide of the formula: 5-(SEQ ID NO: 1)-(formula III)-(SEQ ID NO: 2)-3 where formula III represents a polynucleotide containing nucleotides 343-1152 of SEQ ID NO: 3. Primers and probes based on the isolated polynucleotide, DNA complementary to any of the polynucleotides, primers or probes, a method of detecting and identifying at least one species or group of mycobacteria, and a kit, box, or coordinated set for conducting the method are also described.
Notice that A pairs with T and G pairs with C when a DNA strand hybridizes with another DNA strand. An RNA molecule can also form a base-paired DNA-RNA duplex molecule with a DNA that has complementary base pairing. The most common source of DNA complementary to an mRNA is the DNA coding strand that was the template for synthesis of the RNA. In DNA-RNA hybrid formation, G base pairs with C, A of the RNA pairs with T of the DNA, and U or the RNA pairs with A of the DNA. The DNA-RNA hybridization reaction is illustrated below: ...
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Looking for hybridization probe? Find out information about hybridization probe. A small molecule of deoxyribonucleic acid or ribonucleic acid that is radioactively labeled and used to identify complementary nucleic acid sequences by... Explanation of hybridization probe
A signal amplification method for detecting a target nucleic acid analyte having a homopolymeric region and a target sequence includes steps of: contacting an analyte under hybridizing conditions with a multiplicity of reporter probes, each reporter probe including a signal region and an oligonucleotide sequence which is complementary to and capable of forming a stable hybrid with the analyte homopolymeric region to form an analyte:reporter probe hybrid; and forming an analyte:capture probe hybrid by contacting the analyte target sequence with a capture probe under hybridizing conditions. The analyte:reporter probe hybrid may formed prior to contacting the analyte target sequence with the capture probe, so the result of contacting the analyte target sequence with the capture probe results in formation of an analyte:reporter probe:capture probe complex. The analyte:capture probe hybrid may be immobilized on a solid generally planar surface in an array format. Multiple reporter probes may form triple
The improvement of existing serological techniques, development of monoclonal antibody technology and the development of new serological approaches are all working together to provide new tools for the detection of disease-causing organisms in fish and crustaceans. Following the introduction of nucleic acid hybridization technique and PCR, it was recognized that the methods offered a sensitive approach to the detection and identification of specific microorganisms as in the case of a bacterial or viral infection in a variety of sample types. Potentially, a characteristic DNA sequence from a single virus particle or cell of a particular organism can be amplified to detectable levels within a short period of time. Conventional diagnostic methods that involve the culture of microorganisms can take days or weeks to complete or very tedious to perform. PCR offers a rapid, very sensitive, very specific and simple alternative. Further developments in immunodiagnostics and emerging technologies such as ...
Hello! Im using an in situ hybridization protocol utilizing DIG-labeled probes and alkaline phosphatase visualization to detect genomic sequences in tissue sections and blood spins. This is working excellently. Now Id like to do the same thing with FITC-labeled probes. The probe sequence is the same, the protocol is basically the same (before visualization steps, of course) - but I dont get any signal. I havent done FISH before but I have used fluorescently labeled antibodies, so there should be no problems in the basic handling of fluorescent components. The probe is commercially made and directly labeled with FITC. I have tried with two separately prepared lots, with no success. The in situ hybridization protocol is pretty standard. Any ideas? How about stability of the FITC label in denaturation, hybridization and washing steps? And how about sensitivity as compared with enzymatic detection? I get a strong signal with the AP system. For FISH, I have one FITC label per an oligonucleotide ...
i. To 3ug aRNA, add 3uL pdN15 (use PCR tubes).. 1. Or 2uL of pdN9 (5ug/uL).. 2. pdN15 = random pentadecamer.. 3. pdN9 = random nonamer.. 4. If you need to re-suspend a new tube of pdN15, use 350uL of water - Poly N (15-mer) 50 OD from Operon (Item # SP180-1) has a conc. of 31.7ug/OD, so total mass of 1585ug. Re-suspending in 350uL water gives a final conc. of 4.5ug/uL.. ii. Bring final volume to 19uL with water.. iii. Mix well and incubate at 70oC for 10min in PCR machine.. 1. Program 70 in Main folder (MJ PCR machine).. iv. Place on ice immediately and leave for at least 5min.. ...
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TY - JOUR. T1 - A hibridizáció és alkalmazása DNS-array rendszerekben. AU - Gyorffy, András. AU - Gyorffy, Balázs. AU - Molnár, Béla. AU - Tulassay, Zsolt. PY - 2005/12/1. Y1 - 2005/12/1. N2 - DNA hybridization arrays measure simultaneously the expression of several genes. First, a known DNA sequence (probe) is fixed on a firm basis. Then the complementer sequence (target sequence) is linked to it during the hybridization process. The target sequence extracted from biological samples is fluorescently, enzimatically or radioactivelly labeled before detection. Higher expression results in higher signal in the detection system. Unlabeled DNA strands can also be detected, as the electronical and optical characteristics of the DNA is altered after complementer hybridization. In this review we summarize the basics of hybridisation and its newest application area in the DNA array systems.. AB - DNA hybridization arrays measure simultaneously the expression of several genes. First, a known DNA ...
To exploit fully the potential of current sequencing technologies for population-based studies, one must enrich for loci from the human genome. Here we evaluate the hybridization-based approach by using oligonucleotide capture probes in solution to enrich for approximately 3.9 Mb of sequence target. We demonstrate that the tiling probe frequency is important for generating sequence data with high uniform coverage of targets. We obtained 93% sensitivity to detect SNPs, with a calling accuracy greater than 99%.
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A laboratory techniques|technique used to determine how similar two strands of single-stranded nucleic acids are to each other by putting them with a th...
SSC Hybridization Solution SSC solution is used for the hybridization of probes to nucleic acids. Contains: 6X SSC, 10% Dextran sulfate, 0.2% SDS, 5X Denhardts solution and sheared salmon sperm DNA... DNA/RNA Hybridization
2.2 Procedure. 1. Liquid hybridization was performed as the LHCD method, that is, isolated RNAs were hybridized in hybridization buffer (1 X Exo I buffer) with double-hairpin probe (aslo named as two-hairpin DNA probes) at 42°C (Li et al. 2012). The hybridization mixture was further incubated with FastDigest BamH I (Fermentas) for 1 h at 37°C according to the manufacturers instructions. 2. The mixture was digested with exonuclease I (NEB) for 5 min at 50°C and then for 1 h at 37°C. The short 50°C step must be performed to disrupt the short five-base-pair stem that remains on the capture oligo after Bam H I cleavage without disrupting hybridization of the miRNA to the capture oligo. 3. The digested mixture was used as the template for PCR, which was performed with KOD-Plus DNA polymerase according to the suppliers instructions. The PCR procedure was for 5 min at 94°C, then for 20 s at 94°C, for 20 s at 65°C, for 10 s at 68°C, X Cycles, and for 5 min at 68°C. The value of X was ...
2.2 Procedure. 1. Liquid hybridization was performed as the LHCD method, that is, isolated RNAs were hybridized in hybridization buffer (1 X Exo I buffer) with double-hairpin probe (aslo named as two-hairpin DNA probes) at 42°C (Li et al. 2012). The hybridization mixture was further incubated with FastDigest BamH I (Fermentas) for 1 h at 37°C according to the manufacturers instructions. 2. The mixture was digested with exonuclease I (NEB) for 5 min at 50°C and then for 1 h at 37°C. The short 50°C step must be performed to disrupt the short five-base-pair stem that remains on the capture oligo after Bam H I cleavage without disrupting hybridization of the miRNA to the capture oligo. 3. The digested mixture was used as the template for PCR, which was performed with KOD-Plus DNA polymerase according to the suppliers instructions. The PCR procedure was for 5 min at 94°C, then for 20 s at 94°C, for 20 s at 65°C, for 10 s at 68°C, X Cycles, and for 5 min at 68°C. The value of X was ...
The unsurpassed affinity, specificity, and rapid binding of yPNA make it the molecule of choice for demanding hybridization assays. Whether your lab is conducting basic research, developing tools or diagnostics, or involved in drug discovery and therapeutics, you should consider yPNA. The benefits of γPNA are being realized in fluorescence in situ hybridization (FISH) to measure chromosomal elements such as telomeres, to detect mRNA and miRNA, for antisense knockdown and gene silencing, as well as viral genotyping. Additionally, researchers have shown that γPNA can be used as to efficiently capture and pull out minute quantities of DNA and RNA from blood and other complex matrices for subsequent PCR and sequence analysis. There is great potential for γPNA in virtually any assay or application that relies on nucleic acid hybridization ...
DNA hybridization experiments demonstrated that the gene clusters encoding the F8 fimbriae (fei) as well as the type I fimbriae (pi/) exist in a single copy on the chromosome of E. coli 018:K5 strain 2980. In conjugation experiments with appropriate donors, the chromosomal site of these gene clusters was determined. The pil genes were mapped close to the gene clusters thr and Jeu controlling the biosynthesis of threonine and leucine, respectively. The fei genes were found to be located close to the galactose operon (gal) between the position 17 and 21 of the E. coli chromosomallinkage map ...
Sweet, R; Goodman, N; Cho, J; Ruprecht, R; Redfield, R; and Spiegelman, S, The presence of unique dna sequences after viral induction of leukemia in mice. (1974). Subject Strain Bibliography 1974. 32 ...
Advanced Cell Diagnostics team of in situ hybridization experts are ready to support you in getting superior results using our RNAscope® hybridization assays.
TY - JOUR. T1 - Evaluation of DNA sequencing ambiguities using tetramethyl-ammonium chloride hybridization conditions. AU - Connors, T. D.. AU - Burn, T. C.. AU - VanRaay, T.. AU - Germino, G. G.. AU - Klinger, K. W.. AU - Landes, G. M.. PY - 1997/6. Y1 - 1997/6. UR - http://www.scopus.com/inward/record.url?scp=0030914895&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0030914895&partnerID=8YFLogxK. M3 - Article. C2 - 9187758. AN - SCOPUS:0030914895. VL - 22. SP - 1088. EP - 1090. JO - BioTechniques. JF - BioTechniques. SN - 0736-6205. IS - 6. ER - ...
At the time of publication of a paper by Hozumi and Tonegawa (1) in 1976, a controversy was raging about the number of germline variable (V) genes and how Ab diversity was generated (2). Protein sequencing had clearly shown that Abs were divided into two contiguous regions: a V region and a constant (C) region. Dreyer and Bennett (3) had earlier proposed the two gene-one polypeptide theory, which implied that the genes would somehow be joined at the DNA or RNA level. A plethora of papers were swimming in the literature, which tried to estimate the number of V genes based on liquid hybridization of RNA to DNA, all of which gave inconclusive results because the RNA was not pure, etc. It was widely accepted that there were a handful of C genes, and probably many handfuls of V genes. If so, how would a V gene find its way next to a C gene?. Out of the blue, Hozumi and Tonegawa (1) published a stunning paper that used newly discovered restriction enzymes to digest DNA. They showed by hybridization ...
The invention discloses and claims a signal amplification method for detecting a target nucleic acid analyte having a homopolymeric region and a target sequence. The method comprises (a) contacting an analyte under hybridizing conditions with a multiplicity of reporter probes, each probe including a signal region and an oligonucleotide sequence which is complementary to, and capable of forming a stable hybrid with the analyte homopolymeric region, whereby the hybridization of multiple reporter probes to the homopolymeric region provides for signal amplification; and (b) forming an analyte:capture probe hybrid by contacting the analyte target sequence with a capture probe under hybridizing conditions.
TSG-6 protein and functional derivatives thereof, DNA coding therefor, expression vehicles, such as a plasmids, and host cells transformed or transfected with the DNA molecule, and methods for producing the protein and the DNA are provided, as well as antibodies specific for the TSG-6 protein; a method for detecting the presence of TSG-6 protein in a biological sample; a method for detecting the presence of nucleic acid encoding a normal or mutant TSG-6 protein; a method for measuring induction of expression of TSG-6 in a cell using either nucleic acid hybridization or immunoassay; a method for identifying a compound capable of inducing the expression of TSG-6 in a cell; and a method for measuring the ability of a cell to respond to TNF.
Unlike optical trapping or electrical trapping, our method doesnt require perturbative force fields such as a strong electric field. It only uses gentle fluid flow to confine and manipulate particles. Were using it now to look at interactions between soft materials like vesicles or cells, such as how does the process of vesicle collision or adhesion happen? Our work tends to be focused on these fundamental questions, but these are materials that are used in foods, personal care products, detergents, and liquid fabric softeners, he said.. Looking ahead to new projects, Schroeder is excited about combining elements of biopolymers with synthetic polymers to look at the additive or new properties that arise. Specifically, theyre using elements from nucleic acid hybridization or base pairing to guide the structure of optoelectronic materials.. For the project in collaboration with Northwestern Universitys Michael Jewett and funded by the Department of Defenses Multidisciplinary University ...
Methods of detecting, probing, mapping and directed sequencing of target nucleic acids are provided using a guide RNA and a Cas9 protein. Methods for detecting the binding of the guide RNA/Cas9 complex to a target nucleic acid where the guide RNA includes a 3 tail sequence that can hybridize to a probe are provided. Methods for detecting the binding of the guide RNA/Cas9 complex to a target nucleic acid where the complex is physically detected are provided.
The ability of labeled DNA or RNA probes to bind with high affinity and specificity to complementary nucleic acid sequences forms the basis of nucleic acid hybridization assays. Immunoassays, on the other hand, exploit the strong and specific interaction between antigens and antibodies. One aspect of this dissertation deals with enhancement of the sensitivity of bioluminescence hybridization assays based on the photoprotein aequorin. This is achieved by introducing, enzymatically, multiple aequorin labels per DNA hybrid. The bound aequorin is determined by its characteristic bioluminescence. An 8-fold improvement in sensitivity is observed with the amplified assay as compared to an assay without the amplification step. Another aspect of this dissertation involves the development of two novel microtiter well-based DNA hybridization assays in which an expressible cDNA fragment encoding firefly luciferase serves as a reporter molecule. The reporter molecule undergoes in vitro expression through coupled
TY - JOUR. T1 - Poliovirus detection in water by cell culture and nucleic acid hybridization. AU - Enriquez, Carlos E.. AU - Abbaszadegan, Morteza. AU - Pepper, Ian L.. AU - Richardson, Kenneth J.. AU - Gerba, Charles P.. N1 - Copyright: Copyright 2015 Elsevier B.V., All rights reserved.. PY - 1993/7. Y1 - 1993/7. N2 - Nucleic acid hybridization has been used to detect viral nucleic acid in water. This type of assay, in contrast with tissue culture assays, may not distinguish between viable and non-viable viruses. We evaluated, by comparison with tissue culture infectivity assay (plaque forming method), the ability of the gene probe assay to detect viable poliovirus 1 (LSc) in well water, autoclaved well water, filter-sterilized well water and autoclaved phosphate buffered saline kept at 37 and 15°C for 75 days, and in dechlorinated tap water held at room temperature. A gradual decline in numbers of poliovirus was observed in all of the samples by cell culture assay. With the exception of ...
Comparative genomic hybridization analysis of adrenocortical tumors.: Comparative genomic hybridization (CGH) is a molecular cytogenetic technique that allows t
TY - JOUR. T1 - Comparison of nucleic acid hybridization and fluorometry for measurement of the relationship between RNA/DNA ratio and growth rate in a marine bacterium. AU - Kerkhof, L.. AU - Ward, B. B.. PY - 1993. Y1 - 1993. N2 - Continuous culture of Pseudomonas stutzeri Zobell, a marine denitrifying bacterium, was used to determine the relationship between growth rate and nucleic acid content. The trend of decreasing RNA content with decreasing growth rate, well known for enteric organisms, was found to occur in P. stutzeri Zobell as well, even at very long generation times such as those thought to occur in the oligotrophic ocean. When assayed by ethidium bromide fluorescence, the total RNA/DNA ratio was linear for generation times between 6 and 60 h. We also developed a 200-bp nucleic acid probe (with species- specific potential) for a portion of the 23S rRNA gene of P. stutzeri Zobell, which was used to quantify rRNA and rDNA by hybridization in the same continuous cultures. The rRNA/rDNA ...
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The accessibility and binding affinity of DNA are two key parameters affecting the hybridization efficiency in surface-based biosensor technologies. Better accessibility will result in a higher hybridization efficiency. Often, mixed ssDNA and mercaptohexanol monolayers are used to increase the hybridization efficiency and accessibility of surface-bound oligonucleotides to complementary target DNA. Here, no mercaptohexanol monolayer was used. We demonstrate by differential microcantilever deflection measurements at different pH that the hybridization efficiency peaks between pH 7.5 and 8.5. At low pH 4.5, hydration and electrostatic forces led to tensile surface stress, implying the reduced accessibility of the bound ssDNA probe for hybridization. In contrast, at high pH 8.5, the steric interaction between neighboring ssDNA strands was decreased by higher electrostatic repulsive forces, bending the microcantilever away from the gold surface to provide more space for the target DNA. Cantilever deflection
Nucleic acid sandwich hybridization assays are provided that incorporate one or a combination of background reduction steps. Those steps include use of a separate capture probe and separation from immobilized capture probes by cleavage and isolation. A very sensitive assay for RNA targets includes both of those steps, plus RNA binary probes, an RNA-directed RNA ligase and amplification by an RNA-directed RNA polymerase. Kits of reagents for performing assays according to this invention are also provided.
Looking for online definition of molecular hybridization in the Medical Dictionary? molecular hybridization explanation free. What is molecular hybridization? Meaning of molecular hybridization medical term. What does molecular hybridization mean?
17. S. Bhuckory, E. Hemmer, Y.-T. Wu, A. Yahia-Ammar, F. Vetrone, and N. Hildebrandt. Core or shell ? Er3+ FRET donors in upconversion nanoparticles. European Journal of Inorganic Chemistry 2017, 5186-5195.. 18. E. Porret, L. Sancey, A. Martín-Serrano, M. Montañez, R. Seemann, A. Yahia-Ammar, H. Okuno, F. Gomez, A. Ariza, N. Hildebrandt, J.-B. Fleury, J.-L. Coll, X. Le Guével. Hydrophobicity of Gold Nanoclusters Influences their Interactions with Biological Barriers. Chemistry of Materials 2017, 29 (17), 7497-7506.. 19. S. Díaz, G. Lasarte Aragones, S. Buckhout-White, X. Qiu, E. Oh, K. Susumu, J. Melinger, A. Huston, N. Hildebrandt, and I.L. Medintz. Bridging Lanthanide to Quantum Dot Energy Transfer with a Short Lifetime Organic Dye. The Journal of Physical Chemistry Letters 2017, 8 (10), 2182-2188.. 20. X. Qiu, J. Guo, Z. Jin, A. Petreto, I. L. Medintz, N. Hildebrandt. Multiplexed Nucleic Acid Hybridization Assays Using Single-FRET-Pair Distance-Tuning. Small 2017, 13, 1700332.. 21. M. ...
TY - CHAP. T1 - Comparative genomic hybridization. AU - Albertson, Donna G.. AU - Pinkel, Daniel. PY - 2015/1/1. Y1 - 2015/1/1. N2 - Cells progress to cancer by the acquisition of genetic and epigenetic alterations that promote growth and survival. The genomic alterations range from mutations in individual nucleotides to rearrangements and changes in the copy number of chromosomal segments or whole chromosomes. The former result in point mutations that inactivate tumor suppressor genes or activate oncogenes, and the latter produce novel fusion genes and/or alter the expression of one or more genes that may individually or co-operatively modify cell behavior. The technique of comparative genomic hybridization (CGH) was first introduced in 1992 as a means to assess copy number changes in genomes (1). In the original implementation of CGH, a test genomic DNA, such as DNA extracted from a tumor, and a reference DNA, typically genomic DNA from normal cells, were differentially labeled and then ...
TY - JOUR. T1 - Suppression subtractive hybridization. AU - Ghorbel, Mohamed T. AU - Murphy, David. PY - 2011. Y1 - 2011. N2 - Comparing two RNA populations that differ from the effects of a single independent variable, such as a drug treatment or a specific genetic defect, can establish differences in the abundance of specific transcripts that vary in a population dependent manner. There are different methods for identifying differentially expressed genes. These methods include microarray, Serial Analysis of Gene Expression (SAGE), and quantitative Reverse-Transcriptase Polymerase Chain Reaction (qRT-PCR). Herein, the protocol describes an easy and cost-effective alternative that does not require prior knowledge of the transcriptomes under examination. It is specifically relevant when low levels of RNA starting material are available. This protocol describes the use of Switching Mechanism At RNA Termini Polymerase Chain Reaction (SMART-PCR) to amplify cDNA from small amounts of RNA. The ...
Summary of Facts and Submissions. I. European patent no. 0 497 784 was granted with 39 claims on the basis of European patent application 90913621.0 (published as WO 91/02817, referred to in this decision as the application as filed) and was opposed on the grounds of Article 100(a) EPC, for lack of novelty and inventive step (Articles 54 and 56 EPC), Article 100(b) EPC and Article 100(c) EPC.. II. Independent claims 1, 12 and 30 as granted read:. 1. Use of an internal standard for the quantitation of at least one target nucleic acid segment contained within a sample in an amplification method, said internal standard comprising on one strand a nucleic acid segment comprising a 5 sequence and a 3 sequence, which sequences provide an upstream primer hybridization site and the complement of a downstream primer hybridization site which are identical to an upstream primer hybridization site and the complement of a downstream primer hybridization site within said target nucleic acid segment, ...
Background: Genome-wide analysis of sequence divergence among species offers profound insights into the evolutionary processes that shape lineages. When full-genome sequencing is not feasible for a broad comparative study, we propose the use of array-based comparative genomic hybridization (aCGH) in order to identify orthologous genes with high sequence divergence. Here we discuss experimental design, statistical power, success rate, sources of variation and potential confounding factors. We used a spotted PCR product microarray platform from Drosophila melanogaster to assess sequence divergence on a gene-by-gene basis in three fully sequenced heterologous species (D. sechellia, D. simulans, and D. yakuba). Because complete genome assemblies are available for these species this study presents a powerful test for the use of aCGH as a tool to measure sequence divergence. Results: We found a consistent and linear relationship between hybridization ratio and sequence divergence of the sample to the ...
Biological RNA generally comprises secondary structure motifs which cause a problem for target RNA detection by isothermal amplification methods. The complexity of the secondary structures makes RNA targets inaccessible for probe hybridization, resulting in decreased sensitivity and selectivity. This is particularly important because the hybridization step of the isothermal amplification method re ...
Biological RNA generally comprises secondary structure motifs which cause a problem for target RNA detection by isothermal amplification methods. The complexity of the secondary structures makes RNA targets inaccessible for probe hybridization, resulting in decreased sensitivity and selectivity. This is particularly important because the hybridization step of the isothermal amplification method re ...
A fluorescently labeled nucleic acid having a hairpin structure between the fluorophore label and a point of attachment to a solid phase is useful as a probe to detect nucleic acid from a sample. The solid phase quenches the fluorophore label when the hairpin structure exists but this quenching is relieved by duplex formation between probe and a sample oligonucleotide. Probes for specific nucleic acid sequences can be immobilized as arrays on solid phase surfaces for detection of multiple nucleic acid sequences simultaneously from electrophoresis gels and from aqueous solutions. These probes and methods for their use can be combined with known solid phases, particularly those used for plasmon surface detection and electron transfer detection of nucleic acid. The probes can be washed and reused, and have other advantageous features over known probe methods.
An in situ molecular hybridization system which will detect retrovirus RNA in the cytoplasm of individual virus-infected cells has been developed. The technique was applied to cells infected with simian sarcoma-leukemia virus, where the virus-specific RNA was detected by hybridization to simian sarcoma-leukemia virus 3H-labeled complementary DNA. The system is useful for detecting viral RNA-containing cells in the presence of an excess of virus-negative cells and for determining which type of cell in a heterogenous population is expressing viral RNA.
TY - JOUR. T1 - Enhancement of surface plasmon resonance sensing for DNA hybridization using colloidal Au attached probe DNA. AU - Yamaguchi, Akira. AU - Juodkazis, Saulius. AU - Matsuo, Shigeki. AU - Misawa, Hiroaki. PY - 2002/2/5. Y1 - 2002/2/5. N2 - In this study, we demonstrate that the Au particle modified probe DNA monolayer can enhance the surface plasmon resonance (SPR) signal for measuring hybridization of unlabeled DNA molecules. The Au particles adsorbed on single stranded (ss)- and double stranded (ds)-DNA monolayers have different optical interaction with surface of Au thin film, and this difference induces the enhancement of the SPR signal.. AB - In this study, we demonstrate that the Au particle modified probe DNA monolayer can enhance the surface plasmon resonance (SPR) signal for measuring hybridization of unlabeled DNA molecules. The Au particles adsorbed on single stranded (ss)- and double stranded (ds)-DNA monolayers have different optical interaction with surface of Au thin ...
TY - JOUR. T1 - Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. AU - Melton, D. A.. AU - Krieg, P. A.. AU - Rebagliati, M. R.. AU - Maniatis, T.. AU - Zinn, K.. AU - Green, M. R.. N1 - Funding Information: ACKNOWLEDGEMENTS We are grateful to D. Ward and B. Mierendorf for sharing their unpublished results. We thank K. Breakey for help in preparing figures. P. K. acknowledges support from the Fogarty International Fellowship program. M.R. is grateful for support from an NSF Predoctoral Fellowship. This work was supported by a grant from the NIH to T.M. and grants from the NIH and the Chicago Community Trust/Searle Scholars Program to D.M. Copyright: Copyright 2010 Elsevier B.V., All rights reserved.. PY - 1984/9/25. Y1 - 1984/9/25. N2 - A simple and efficient method for synthesizing pure single stranded RNAs of virtually any structure is described. This in vitro transcription system is based on the ...
There is merit in each of these predictions. For example, profiling has re-emerged in clinical testing in the form of protein, tissue and nucleic acid arrays (e.g., cytokine profiles, array comparative genomic hybridization analysis) [49-52]. Computers and automation have played an increasingly important role in improving the efficiency and effectiveness of testing. More recently, pharmacogenetics, popularized by the slogan right patient, right drug, right time [53], has moved into mainstream testing (e.g., CYP2C9 for warfarin dosing) [54]. Since 1969, there have been many predictions and views of the future development of laboratory medicine and its sub-specialties. A summary of these predictions is provided in Table 1 [12-48]. A number of common themes and buzz-words can be identified in the prognostications such as nanotechnology, biosensors, microchips, genomics, and proteomics. These topics, together with the more specific predictions, are discussed in greater detail below. ...
TY - JOUR. T1 - A double labeling technique for performing immunocytochemistry and in situ hybridization in virus infected cell cultures and tissues. AU - Gendelman, Howard E.. AU - Moench, Thomas R.. AU - Narayan, Opendra. AU - Griffin, Diane E.. AU - Clements, Janice E.. PY - 1985/1/1. Y1 - 1985/1/1. N2 - This report describes a combined immunocytochemical and in situ hybridization procedure which allows visualization of cellular or viral antigens and viral RNA in the same cell. Cultures infected with visna or measles virus were fixed in periodate-lysine-paraformaldehyde-glutaraldehyde, stained by the avidin-biotin-peroxidase technique using antibodies to viral or cellular proteins and then incubated with radiolabeled specific DNA probes (in situ hybridization). The immunoperoxidase stain was preserved through the hybridization procedure. Nonspecific sticking of probes over peroxidase stained cells was prevented by incorporation of 0.1% Triton X-100 into the hybridization solution and the ...
We have confirmed and better defined the presence of a potentially important marker for malignant progression of BE. Furthermore, we have narrowed the area from 30 Mb, i.e., 7q33-q35 in our previous comparative genomic hybridization study (7) , to ∼2 Mb, i.e., the region between markers D7S2439 and D7S483. It implies the presence of a possible biomarker, which, in addition, has tumor suppressive activities. The 7q32.3-q36.1 region has not been reported frequently to be lost in human cancers. Thus far, it has been observed in gallbladder tumors, oral and oropharyngeal epithelial carcinomas, and leukemia (8, 9, 10) . In gallbladder tumors ,60% of allelic imbalance was seen for marker D7S798. Interestingly, it is located between D7S483 and D7S2465. We screened the critical area for known genes 7 with tumor suppressive potential and selected two possible candidates. Caspase 2 is known to stimulate apoptosis and is involved in shedding of intestinal epithelium (11) . Loss of these functions could ...
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Comparative genomic hybridization (CGH) is a modern genetic method which enables a genome‐wide survey of chromosomal imbalances. For each chromosome region, one obtains the information whether there is a loss or gain of genetic material, or whether t
Array hybridization devices and methods for their use are provided. The subject devices are characterized by having a substantially planar bottom surface, a cover, at least one fluid port and at least one adjustable spacing element for adjusting the spacing between an array and the bottom surface. In using the subject devices, an array is placed on the at least one adjustable spacing element in the chamber and the space between the array and the bottom surface is adjusted by moving the at least one adjustable spacing element. The adjusted array is contacted with at least one biological sample introduced into the chamber. The subject inventions find use in a variety of array-based applications, including nucleic acid array hybridizations.
|p>Decrease any non-specific hybridization of a probe to a substrate in Northern, Southern, or other nucleic acid hybridisation protocols. The Placentas used for obtaining the DNA come from healthy donors negative for the following: Hep-B & Hep C; HIV-1 and HIV-2; HTLV-1 and HTLV-1; Treponema Pallidum|/p>
The present invention includes polynucleotides encoding an MCP1 (e.g., a mature MCP1 polypeptide), an MCP1 multimer or a fusion thereof (e.g., fused to an in vivo half-life extending moiety (e.g., Ig)) (e.g., any of SEQ ID NOs: 1, 2, 8-12) as well as nucleic acids which hybridize to the polynucleotides. Preferably, the nucleic acids hybridize under low stringency conditions, more preferably under moderate stringency conditions and most preferably under high stringency conditions. A nucleic acid molecule is hybridizable to another nucleic acid molecule, such as a cDNA, genomic DNA, or RNA, when a single stranded form of the nucleic acid molecule can anneal to the other nucleic acid molecule under the appropriate conditions of temperature and solution ionic strength (see Sambrook, et a/., supra). The conditions of temperature and ionic strength determine the stringency of the hybridization. Typical low stringency hybridization conditions are 55°C, 5X SSC, 0.1% SDS, 0.25% milk, and no ...
Paraffin sections of human skeletal tissues were studied in order to identify cells responsible for production of types I, II, and III collagens by in situ hybridization. Northern hybridization and sequence information were used to select restriction fragments of cDNA clones for the corresponding mRNAs to obtain probes with a minimum of cross-hybridization. The specificity of the probes was proven in hybridizations to sections of developing fingers: osteoblasts and chondrocytes, known to produce only one type of fibrillar collagen each (I and II, respectively) were only recognized by the corresponding cDNA probes. Smooth connective tissues exhibited variable hybridization intensities with types I and III collagen cDNA probes. The technique was used to localize the activity of type II collagen production in the different zones of cartilage during the growth of long bones. Visual inspection and grain counting revealed the highest levels of pro alpha 1(II) collagen mRNAs in chondrocytes of the ...
This Decision Document has been prepared to explain the regulatory decision reached under the guidelines Dir94-08 Assessment Criteria for Determining Environmental Safety of Plants with Novel Traits and its companion document Dir94-09 The Biology of Brassica napus L. (Canola/Rapeseed), and the proposed guidelines Pro94-04 Guidelines for the Assessment of Plants with Novel Traits as Livestock Feed.
This Decision Document has been prepared to explain the regulatory decision reached under the guidelines Dir94-08 Assessment Criteria for Determining Environmental Safety of Plants with Novel Traits and its companion document Dir94-09 The Biology of Brassica napus L. (Canola/Rapeseed) and the guidelines Dir95-03 Guidelines for the Assessment of Livestock Feed from Plants with Novel Traits.
This Decision Document has been prepared to explain the regulatory decision reached under the guidelines Dir94-08 Assessment Criteria for Determining Environmental Safety of Plants with Novel Traits and its companion document Dir94-09 The Biology of Brassica napus L. (Canola/Rapeseed) and the guidelines Dir95-03 Guidelines for the Assessment of Livestock Feed from Plants with Novel Traits.
В немецком издательстве Springer Verlag вышла книга Fluorescence In Situ Hybridization (FISH). Application Guide (с годом издания 2017). Это второе издание сборника протоколов, который был выпущен в 2009 году. Три главы из этой книги написаны сотрудниками Отдела разнообразия и эволюции геномов ИМКБ или при их участии.. Yang F, Trifonov V, Ng BL, Kosyakova N, Carter NP. Generation of paint probes from flow-sorted and microdissected chromosomes. pp. 63-79. Trifonov VA, Vorobieva NV, Serdyukova NA, Rens W. FISH with and without COT1 DNA. pp. 123-132. Yang F, Graphodatsky AS. Animal probes and ZOO-FISH. pp. 395-415. This manual offers detailed protocols for fluorescence in situ hybridization (FISH) and comparative genomic hybridization approaches, which have been successfully used to study various aspects of ...
TY - JOUR. T1 - Drugging the R-loop interactome. T2 - RNA-DNA hybrid binding proteins as targets for cancer therapy. AU - Boros-Oláh, Beáta. AU - Dobos, Nikoletta. AU - Hornyák, Lilla. AU - Szabó, Zoltán. AU - Karányi, Zsolt. AU - Halmos, Gábor. AU - Roszik, Jason. AU - Székvölgyi, Lóránt. PY - 2019/12. Y1 - 2019/12. N2 - Unravelling the origin of genetic alterations from point mutations to chromosomal rearrangements was greatly enhanced by the discovery of RNA-DNA hybrids (R-loops) that behave as hotspots of genomic instability in a variety of organisms. Current models suggest that uncontrolled R-loops are a hazard to genome integrity, therefore, identifying proteins that are involved in recognising and signalling R-loop structures are of key importance. Herein we analysed key RNA-DNA hybrid binding proteins in humans taking advantage of large-scale gene expression, survival rate, and drug-sensitivity data from cancer genomics databases. We show that expression of RNA-DNA hybrid ...
TY - JOUR. T1 - Kinetics of spontaneous displacement of RNA from heteroduplexes by DNA. AU - Landgraf, Ralf. AU - Ramamurthi, Kumaran S.. AU - Sigman, David S.. PY - 1996/9/4. Y1 - 1996/9/4. N2 - We have used R-loop formation and direct hybridization techniques to analyze the kinetics by which RNA is displaced from a heteroduplex by DNA of identical sequence. Using random walk simulations we were able to calculate the step times for a single displacement reaction. For RNA with a GC content of 57-60% the data indicate an RNA exchange probability of 50.06%, which is indicative of a modest destabilization of the heteroduplex compared with a DNA duplex in the presence of magnesium. The average step time for the reversible exchange of a single nucleotide is 345.0 (± 1.3) ms/step. An acceleration of the displacement reaction was observed in the absence of magnesium. A comparison with step times for elongation shows that RNA displacement would not be rate limiting to transcription elongation under two ...
Rare DNA-sequence variants hold important clinical and biological information, but existing detection techniques are expensive, complex, allele-specific, or dont allow for significant multiplexing. Here, we report a temperature-robust polymerase-chain-reaction method, which we term blocker displacement amplification (BDA), that selectively amplifies all sequence variants, including single-nucleotide variants (SNVs), within a roughly 20-nucleotide window by 1,000-fold over wild-type sequences. This allows for easy detection and quantitation of hundreds of potential variants originally at ≤0.1% in allele frequency. BDA is compatible with inexpensive thermocycler instrumentation and employs a rationally designed competitive hybridization reaction to achieve comparable enrichment performance across annealing temperatures ranging from 56 °C to 64 °C. To show the sequence generality of BDA, we demonstrate enrichment of 156 SNVs and the reliable detection of single-digit copies. We also show that the BDA
In article ,1996Feb14.150838 at opal.tufts.edu,, kmorris1 at opal.tufts.edu (KELLY THOME) wrote: , Im hoping someone can help me with remedial Southern analysis technique :-). , Imtrying, without much success, to do low stringency Southerns (ie, hunting for , family members in one species/looking for homologs in other species). My hyb , and washing conditions for high stringency blotting are pretty standard, I , guess... hyb is 6X SSC/10X Dextran Sulfate/ 0.5% SDS/ 50micrograms/ml ssDNA at , 65 degrees, washing is 1X SSC/0.5% SDS at 65 degrees. I tried lowering the , SDS to 0.1% and hybing at 42, but then I got NO signal, not even the signal I , see at high stringency. Im getting sort of frustrated at blank blots and , would appreciate advice from someone with experience in this sort of thing. , Thanks in advance! , -Kelly I guess the next thing to do is to keep lowering the hybridisation temperature until you do get some hybridisation. I have successfully done low stringency Southerns using ...
Generation of bovine RPE/retina-subtracted cDNA library. Detailed description of the library will be published elsewhere (J. T. Chang, N. Della, C. Chew, S. Zhang, P. A. Campochiaro, and D. J. Zack, unpublished data). In brief, a library was constructed in Uni-ZAP XR (Strategene, La Jolla, CA) using cDNA that was generated from bovine RPE RNA; the library was in vivo excised and made single-stranded, hybridized in several rounds with an excess of biotinylated heart and liver RNA; the resulting RNA-DNA hybrids and unhybridized RNA were removed by phenol extraction after the addition of streptavidin; and the remaining unhybridized plasmid DNA was electroporated into MC1061 cells.. Fluorescent in situ hybridization. Fluorescentin situ hybridization (FISH) mapping was performed by standard methods (Lichter et al., 1990). Identical results were obtained with two independent but overlapping P1 clones. The clones were identified from high-density filters and were processed according to the suppliers ...
Advertisement Molecular Pathology. Microarray. Variations Tissue microarray. Author: Rodney E. Shackelford, DO, Ph.D. (see Reviewers page). Revised: 3 July 2010, last major update July 2010. Copyright: (c) 2008-2010, PathologyOutlines.com, Inc.. General. =========================================================================. ● Tissue microarray (TMA) significantly differs from the other techniques discussed in this chapter because the hybridization step involves antibody binding to one target protein, or nucleic acid hybridization to one target gene. ● TMA allows for the simultaneous analysis of protein expression in up to 500 different tissue samples. Advantages. =========================================================================. ● The relative level of a specific protein s expression can be compared on the same slide, allowing uniform analysis. ● A small amount of tissue is used per sample analyzed, allowing more analyses per same tissue volume and lowering the amount of ...
Fingerprint Dive into the research topics of Identification of underexpressed genes in early- and late-stage primary ovarian tumors by suppression subtraction hybridization. Together they form a unique fingerprint. ...
Hybrid organism is produced by interbreeding between two animals or plants of same or different taxa. This process of hybrid formation is hybridization.
A method of detecting a target nucleic acid A is disclosed, comprising hybridizing the target nucleic acid A with a probe nucleic acid B which contains a sequence B1 which base pairs with a part of the target nucleic acid A and a sequence B2, cleaving the hybridized probe nucleic acid B to produce a cleavage product B containing the sequence B2, hybridizing the cleavage product B with a template nucleic acid C containing a sequence C2 which base pairs with a part of the cleavage product B and a sequence C1 which does not hybridize with the sequence B1 of the probe nucleic acid B, extending the hybridized cleavage product B with an extension sequence B3 which is template-specific to a part of the sequence C1, hybridizing a probe D with the extension product, wherein the probe D contains a sequence D1 which base pairs with the extension sequence B3 and a sequence D2, and detecting any of the various products formed throughout the method. Products for performing the method are also disclosed.
Researchers have for the first time determined that hybridization between two bird species can give rise to several novel and fully functional hybrid genomic combinations. This could potentially be because hybrid species emerged through independent hybridisation events between the same parent species on different islands.
"Nucleic Acid Hybridization - MeSH - NCBI". www.ncbi.nlm.nih.gov. Retrieved 16 November 2020. Wang, Qiaochun; Mawassi, Munir; Li ... The fifth ORF codes for nucleic-acid binding protein. This is the protein that helps the DNA or RNA connect with amino acids. ... Nucleic acid hybridizationis also used to detect GVA. In this method, a small sample of the infected plant is taken and then ... "Nucleic Acid Binding Protein - an overview , ScienceDirect Topics". www.sciencedirect.com. Retrieved 16 November 2020. "RPO132 ...
Markham NR, Zuker M (2008). UNAFold: software for nucleic acid folding and hybridization. Methods Mol Biol. Methods in ... "Sfold web server for statistical folding and rational design of nucleic acids". Nucleic Acids Res. 32 (Web Server issue): W135- ... software to identify motifs and short-range interactions in trajectories of nucleic acids". Nucleic Acids Research. 43 (17): ... Wilm A, Higgins DG, Notredame C (May 2008). "R-Coffee: a method for multiple alignment of non-coding RNA". Nucleic Acids Res. ...
Noyes, Barbara E.; Stark, George R. (July 1975). "Nucleic acid hybridization using DNA covalently coupled to cellulose". Cell. ... and thus crucial for oxidizing ascorbic acid. Through inhibition studies with p-chloro-mercuribenzoic acid, Stark came to the ... Essentially, cyanate reacts with the amino groups and exposes them in order for them to react with acid and form hydantoins, ... Using chromatography, Stark was able to detect a change in the amino acid sequence of ribonuclease, specifically the loss of ...
Web server for nucleic acid folding and hybridization prediction. Retrieved on 2020-8-01. RBPmap: mapping binding sites of RNA ... December 2014). "Chk2 and REGγ-dependent DBC1 regulation in DNA damage induced apoptosis". Nucleic Acids Research. 42 (21): ... Isoform 1 has 199 amino acid residues and a domain named DUF4517. Isoform 2 has 174 amino acid residues, and isoform X1 has 154 ... Overall, the positively charged amino acid residues in human protein C20orf27 outnumbers the negatively charged amino acid ...
Zuker, M (2003). "Mfold web server for nucleic acid folding and hybridization prediction". Nucleic Acids Research. 13 (31): ... Nucleic Acids Research. 45 (D1): D200-D203. doi:10.1093/nar/gkw1129. PMID 27899674. "Predict Location of Transmembrane Helices ... Chou, P. Y.; Fasman, G. D. (1978). "Prediction of the secondary structure of proteins from their amino acid sequence". Advances ... The longest polypeptide of transmembrane protein 217 consists of 229 amino acids. This protein isoform has a predicted weight ...
Zuker, M. (1 July 2003). "Mfold web server for nucleic acid folding and hybridization prediction". Nucleic Acids Research. 31 ( ... "Factors determining peripheral vein tolerance to amino acid infusions". Archives of Surgery. 114 (8): 897-900. doi:10.1001/ ...
Zuker M (July 2003). "Mfold web server for nucleic acid folding and hybridization prediction". Nucleic Acids Research. 31 (13 ... Nucleic Acids Research. 34 (12): 3484-3493. doi:10.1093/nar/gkl453. PMC 1524904. PMID 16870723.[dead link] Sun X, Zhulin I, ... Nucleic Acids Research. 30 (17): 3662-3671. doi:10.1093/nar/gkf508. PMC 137430. PMID 12202750. Pelly S, Bishai WR, Lamichhane G ... Nucleic Acids Research. 31 (22): 6435-6443. doi:10.1093/nar/gkg867. PMC 275561. PMID 14602901. Kawano M, Reynolds AA, Miranda- ...
Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res. 31 (13), 3406-15, (2003) " ... Nucleic Acids Res. 25:3389-3402. "TimeTree :: The Timescale of Life". www.timetree.org. Retrieved 2017-05-12.. ... Amino acids serine and lysine are highly represented in the protein at a higher frequency than observed in most proteins in ... BPS : A. W. Burgess and P. K. Ponnuswamy and H. A. Sheraga, Analysis of conformations of amino acid residues and prediction of ...
Zuker M (July 2003). "Mfold web server for nucleic acid folding and hybridization prediction". Nucleic Acids Research. 31 (13 ... July 2019). "The EMBL-EBI search and sequence analysis tools APIs in 2019". Nucleic Acids Research. 47 (W1): W636-W641. doi: ... Isoform 1 is 419 amino acids long and is the most abundant form. Isoform 2 is 225 amino acids, containing only 11 exons and a ... The amino acid composition of BZW2 has a higher amount of lysines and a lower amount of prolines in humans but a higher ...
Zuker M (July 2003). "Mfold web server for nucleic acid folding and hybridization prediction". Nucleic Acids Research. 31 (13 ... Zhang Y, Skolnick J (2005-04-11). "TM-align: a protein structure alignment algorithm based on the TM-score". Nucleic Acids ... January 2020). "ELM-the eukaryotic linear motif resource in 2020". Nucleic Acids Research. 48 (D1): D296-D306. doi:10.1093/nar/ ... Yang J, Zhang Y (July 2015). "I-TASSER server: new development for protein structure and function predictions". Nucleic Acids ...
The common nucleic acid detection method includes direct DNA hybridization. The direct DNA hybridization approach is the ... Dunbar, Sherry A. (2006). "Applications of Luminex xMAP technology for rapid, high-throughput multiplexed nucleic acid ... Hybridization between the capture probe and the target DNA is achieved by melting and annealing complementary target DNA ... Probe-target hybridization is usually detected by optically labeled targets, which determines the relative abundance of each ...
Nucleic acid hybridization tests (DNA probe test) also find Chlamydia DNA. A probe test is very accurate, but is not as ... Nucleic acid amplification tests (NAATs) tests find the genetic material (DNA) of Chlamydia bacteria. These tests are the most ... A polymerase chain reaction (PCR) test is an example of a nucleic acid amplification test. This test can also be done on a ... Fox, A., Rogers, J. C., Gilbart, J., Morgan, S., Davis, C. H., Knight, S., & Wyrick, P. B. (1990). Muramic acid is not ...
關於Nucleic acid hybridization. 的圖書館資源 *Resources in your library ... Nucleic Acids Research. 1987, 15 (13): 5069-5083. PMC 305948. PMID 3601667. doi:10.1093/nar/15.13.5069.. ... Nucleic Acids Res. 1990, 18 (21): 6409-6412. PMC 332522. PMID 2243783. doi:10.1093/nar/18.21.6409.. ... The Phylogeny of the Hominoid Primates, as Indicated by DNA-DNA Hybridization. Journal of Molecular Evolution. 1984, 20 (1): 2- ...
His work played a pivotal role in the discovery of nucleic acid hybridization. In 1955, Rich and Crick solved the structure of ... the importance of RNA and the development of nucleic acid hybridization A Conversations with Alex Rich (10/03/2007) Cold Spring ... the importance of RNA and the development of nucleic acid hybridization". MIT Department of Biology. 31 May 2018. Retrieved 21 ... The Discovery of Polynucleotide Hybridization MIT Article: Alexander Rich, ...
Khodakov D, Wang C, Zhang DY (October 2016). "Diagnostics based on nucleic acid sequence variant profiling: PCR, hybridization ... Khodakov D, Wang C, Zhang DY (October 2016). "Diagnostics based on nucleic acid sequence variant profiling: PCR, hybridization ... For example, because cell-free nucleic acids exist in human plasma, a simple blood sample can be enough to sample genetic ... Many, but not all, molecular diagnostics methods based on nucleic acids detection use polymerase chain reaction (PCR) to vastly ...
This dark tonality only appears when nucleic acid hybridisation occurs. Coconut cadang-cadang disease has no treatment yet. ... The first step is the purification to obtain the nucleic acids of the plant cells. The leaves of the plant located four or more ... Coconuts from Asia and South Pacific have been found to have viroids with similar nucleic acid sequences of CCCVd. The ... the nucleic acids can be extracted by chloroform procedures, for example. When approximately 1 g of coconut tissue has been ...
"Label-free detection of DNA hybridization based on hydration-induced tension in nucleic acid films". Nature Nanotechnology. 3 ( ...
The virus can be detected with ELISA, immunodiffusion tests, or a nucleic acid hybridization assay. The Cymbidium mosaic virus ... "Detection and localization of viruses in orchids by tissue-print hybridization". Plant Pathology. 41 (3): 355-361. doi:10.1111/ ...
Fusion protein Gene pool Gene flow Introgression Nucleic acid hybridization Mouse models of breast cancer metastasis "Transgene ... They were persistent over a 6-year study period, without herbicide selection pressure and despite hybridization with the wild ... The escape of genetically-engineered plant genes via hybridization with wild relatives was first discussed and examined in ... sympatry and weedcrop in situ hybridization". Canadian Journal of Botany. 84 (12): 1842-1851. doi:10.1139/b06-135. Warwick, S.I ...
... but can also arise from polyploidisation or nucleic acid hybridization. Over evolutionary time, if the function of the new ... In particular, amino acid substitutions that change the electric charge of the enzyme are simple to identify by gel ... Alternatively, if the amino acid residue that is changed is in a relatively unimportant part of the enzyme (e.g., a long way ... The enzyme is a monomer, the isoenzymes are due to the differences in the carbohydrate content (sialic acid residues). The most ...
Genetic Relatedness among Strains of Acholeplasma laidlawii and of Acholeplasma axanthum by Nucleic Acid Hybridization" (PDF). ...
... the importance of RNA and the development of nucleic acid hybridization". MIT Department of Biology. 2018-05-31. Retrieved 2020 ... In 1943, Edsall and Cohn published a physical chemistry book Proteins, Amino Acids and Peptides, that had a profound influence ...
"Simplified high throughput protocol for Northern hybridization". Nucleic Acids Research. 21 (14): 3337-3338. doi:10.1093/nar/ ... Probes for northern blotting are composed of nucleic acids with a complementary sequence to all or part of the RNA of interest ... In this procedure, the substrate nucleic acid (that is affixed to the membrane) is a collection of isolated DNA fragments, and ... Acids Research. 32: e175. Gortner, G.; Pfenninger, M.; Kahl, G.; Weising, K. (1996). "Northern blot analysis of simple ...
Among his best-known work is his research on nucleic acid hybridization, much of which was conducted in along with Kim Atwood ... His research in this time focused on nucleic acids and particularly on the enzymes associated with nucleic acid synthesis, ... He developed the technique of nucleic acid hybridization, which helped to lay the groundwork for advances in recombinant DNA ... Rich A; Davies DR (1956). "A new, 2-stranded helical structure, polyadenylic acid and polyuridylic acid". J. Am. Chem. Soc. 78 ...
Enzymes are used to indicate the extent of hybridization but are not used to manipulate the nucleic acids. Thus, small amounts ... "A branched DNA signal amplification assay for quantification of nucleic acid targets below 100 molecules/ml". Nucleic Acids ... The branched DNA binds to the sample nucleic acid by specific hybridization in areas which are not occupied by capture hybrids ... The design of the branched DNA and the way it is hybridized to the nucleic acid to be investigated differs between different ...
In 1966 Max L. Birnstiel and collaborators showed via nucleic acid hybridization experiments that DNA within nucleoli code for ... Nucleic Acids and Protein Synthesis. 114 (2): 296-310. doi:10.1016/0005-2787(66)90311-x. PMID 5943882. Sirri V, Urcuqui-Inchima ...
"Direct selection of human genomic loci by microarray hybridization". Nucleic Acids Res. 33 (21): e183. doi:10.1093/nar/gni177. ... Nucleic Acids Res. 33 (8): e71. doi:10.1093/nar/gni070. PMC 1087789. PMID 15860768. Bashiardes S, Veile R, Helms C, Mardis ER, ... Nucleic Acids Res. 35 (7): e47. doi:10.1093/nar/gkm078. PMC 1874629. PMID 17317684. Dahl F, Gullberg M, Stenberg J, Landegren U ... fluorescence in situ hybridization (FISH) or array comparative genomic hybridization (aCGH). MIP has been used extensively in ...
Differential characteristics Commercially available nucleic acid hybridisation assays are widely used to identify members of ... This mycobacterium species was first described as a pathogen of field voles in England Gram-positive, nonmotile, acid-fast rods ...
"Candida bracarensis Detected among Isolates of Candida glabrata by Peptide Nucleic Acid Fluorescence In Situ Hybridization: ...
Nucleic Acids Res. November 2002, 30 (21): 4634-42. PMC 135794. PMID 12409453. doi:10.1093/nar/gkf587.. ... Chromosomal assignment of human nuclear envelope protein genes LMNA, LMNB1, and LBR by fluorescence in situ hybridization. ... a 50-amino acid deletion in prelamin A (amino acids 607-656) removes the site for the second endoproteolytic cleavage. ...
... is a molecular cytogenetic technique that uses fluorescent probes that bind to only those parts of a nucleic acid sequence with ... Wikimedia Commons has media related to Fluorescence in situ hybridization.. *Fluorescent+in+Situ+Hybridization at the US ... Comparative genomic hybridization[edit]. Comparative genomic hybridization can be described as a method that uses FISH in a ... The hybridization signals for each probe when a nucleic abnormality is detected.[9] Each probe for the detection of mRNA and ...
"Nucleic Acids Research 22 (17).. *↑ Un bo recurso de introdución a EMBnet é a páxina What is EMBnet? Arquivado 07 de setembro ... Olshen, A. B.; Venkatraman, E. S. (2002). "Change-point analysis of array-based comparative genomic hybridization data". ... "Nucleic Acids Research 4 (11). Páxs. 4037-4051.. *↑ 33,0 33,1 Sanger, F.; et al. (1978). "The nucleotide sequence of ... "Nucleic Acids Research 22 (22). Páxs. 4673-80.. *↑ Altschul, S. F.; et al. (1997). "Gapped BLAST and PSI-BLAST: a new ...
After hybridization, excess probe is washed from the membrane (typically using SSC buffer), and the pattern of hybridization is ... If some of the DNA fragments are larger than 15 kb, then prior to blotting, the gel may be treated with an acid, such as dilute ... Modification of the hybridization conditions (for example, increasing the hybridization temperature or decreasing salt ... Gel electrophoresis of nucleic acids. *Restriction fragment. *Genetic fingerprint. *Northern blot. *Western blot ...
"Nucleic Acids Res. 27 (11): 2291-8. PMC 148793 . PMID 10325416. doi:10.1093/nar/27.11.2291.. ... fluorescent in situ hybridization, methylation-sensitive restriction enzymes, DNA adenine methyltransferase identification ( ... "Nucleic Acids Research. 40 (22): 11450-62. PMC 3526280 . PMID 23034806. doi:10.1093/nar/gks891.. ... Histone proteins are made up of long chains of amino acids. If the amino acids that are in the chain are changed, the shape of ...
"Nucleic Acids Res. 17 (4): 1733-43. doi:10.1093/nar/17.4.1733. PMC 331831. PMID 2522186.. ... SNRPN-methylation is used to detect uniparental disomy of chromosome 15.[6] After fluorescent-in-situ-hybridization has ... "Nucleic Acids Res. 27 (23): 4577-84. doi:10.1093/nar/27.23.4577. PMC 148745. PMID 10556313.. ... Although individual snRNPs are believed to recognize specific nucleic acid sequences through RNA-RNA base pairing, the specific ...
"Nucleic Acids Research. 42 (14): 8845-60. doi:10.1093/nar/gku555. PMC 4132710. PMID 25053837.. ... by employing fluorescent in situ hybridization (FISH) and/or post-sequencing confirmation.[10] The bias of MDA against high %GC ... "Nucleic Acids Research. 40 (17): e136. doi:10.1093/nar/gks454. PMC 3458524. PMID 22649061.. ... In addition, due to the picogram level of the amount of nucleic acids used,[4] heavy amplification is often needed during ...
Nucleic acid denaturationEdit. Main article: Nucleic acid thermodynamics. Nucleic acids (including RNA and DNA) are nucleotide ... "Characterization of denaturation and renaturation of DNA for DNA hybridization". Environmental Health and Toxicology Environ ... of formamide denatured nucleic acids are similar to those of heat-denatured nucleic acids.[22][24][25] Therefore, depending on ... "Calculation of hydrodynamic properties of small nucleic acids from their atomic structure". Nucleic Acids Research. 30 (8): ...
"Nucleic Acids Research. 10 (8): 2709-21. doi:10.1093/nar/10.8.2709. PMC 320645 . PMID 7079182.. ... Sequencing by hybridization[edit]. Sequencing by hybridization is a non-enzymatic method that uses a DNA microarray. A single ... "Nucleic Acids Research. 1 (3): 331-53. doi:10.1093/nar/1.3.331. PMC 344020 . PMID 10793670.. ... "Nucleic Acids Research. 6 (7): 2601-10. doi:10.1093/nar/6.7.2601. PMC 327874 . PMID 461197.. ...
... essentially it must amplify its own nucleic acids in order to cause a disease. This amplification of nucleic acid in infected ... Acids, alcohols and gases are usually detected in these tests when bacteria are grown in selective liquid or solid media. ... The acid-fast staining procedure identifies the Actinobacterial genera Mycobacterium and Nocardia. ... Two methods, the Gram stain and the acid-fast stain, are the standard approaches used to classify bacteria and to diagnosis of ...
Hasler J, Strub K, (2006). "Alu elements as regulators of gene expression". Nucleic Acids Research 34 (19): 5491-5497 ... to human chromosome 2q14.1 by in situ hybridization". Cytogenet Cell Genet 84 (1-2): 91-2 ...
"Molecular structure of nucleic acids; a structure for deoxyribose nucleic acid" (PDF). Nature. 171 (4356): 737-8. Bibcode: ... In his paper "Versuche über Pflanzenhybriden" ("Experiments on Plant Hybridization"), presented in 1865 to the Naturforschender ... "Independent functions of viral protein and nucleic acid in growth of bacteriophage". The Journal of General Physiology. 36 (1 ... corresponds either to one of the twenty possible amino acids in a protein or an instruction to end the amino acid sequence; ...
"Nucleic Acids Res. (Maskos U, Southern EM.) 20 (7): 1679-84. PMC 312256. PMID 1579459. doi:10.1093/nar/20.7.1679. Check date ... "Oligonucleotide hybridizations on glass supports: a novel linker for oligonucleotide synthesis and hybridization properties of ...
"Nucleic Acids Research. 32 (20): e164. doi:10.1093/nar/gnh163. PMC 534642. PMID 15561999.. ... A detection system that records and interprets the hybridization signal.. The ASO probes are often chosen based on sequencing ... Sheils, O; Finn, S; O'Leary, J (2003). "Nucleic acid microarrays: An overview". Current Diagnostic Pathology. 9 (3): 155-8. doi ... Nucleic Acids Research. 37 (13): 4181-4193. doi:10.1093/nar/gkp552.. *^ Rapley, Ralph; Harbron, Stuart (2004). Molecular ...
"Nucleic Acids Res. 26 (14): 3372-3378. doi:10.1093/nar/26.14.3372. PMC 147699. PMID 9649621.. ... which employs a hybridization protection assay to distinguish Chlamydia trachomatis infections.[14] Various detection and ... 1996). Progress in Nucleic Acid Research and Molecular Biology. Academic Press. pp. 280-287. ISBN 978-0-12-540054-1.. ...
Nucleic Acids Res.. . 17, Nr. 22, 1990, S. 9113-26. doi:10.1093/nar/17.22.9113. PMID 2587254. PMC 335118 (freier Volltext). ... Chromosomal localization of the human histone H2A.X gene to 11q23.2-q23.3 by fluorescence in situ hybridization. . In: Hum. ...
Nucleic Acids Research, 1974. *0. EKWUEME ja A. P. M. FORREST, Release of an Immunologically Active Humoral Factor from the ... PCR in situ hybridization, and RNA in situ hybridization, The Journal of Pathology, 197. väljaanne, nr 5, lk 684-688, august ... 1975 Nucleic Acids Research, 1975. *Senelar R, Escola MJ, Escola R, Serrou B, Serre A., Relationship between Hassall's ... Mulder GB, Manley N, Maggio-Price L., Retinoic acid-induced thymic abnormalities in the mouse are associated with altered ...
"Nucleic Acids Research. 37 (Database issue): D141-D145. doi:10.1093/nar/gkn879. PMC 2686447. PMID 19004872.. ... DNA-DNA hybridisation,[84] which corresponds to less than 97% 16S DNA sequence identity.[85] It has been noted that if this ... "Nucleic Acids Research. 19 Suppl: 2017-2021. doi:10.1093/nar/19.suppl.2017. PMC 331344. PMID 2041798.. ... "Nucleic Acids Research. 35 (21): 7188-7196. doi:10.1093/nar/gkm864. PMC 2175337. PMID 17947321.. ...
"Nucleic Acids Res. 26 (3): 847-53. doi:10.1093/nar/26.3.847. PMC 147327. PMID 9443979.. ... by multi-capillary electrophoresis or also dedicated oligonucleotides array based on comparative genomic hybridization (array- ... In their unanimous decision on October 7, 2015 the "high court found that an isolated nucleic acid, coding for a BRCA1 protein ... fatty acid metabolic process. • positive regulation of gene expression. • negative regulation of histone H3-K4 methylation. • ...
A structure for deoxyribose nucleic acids. Nature 171:737-738. doi:10.1038/171737a0 ... Gregor Mendel (1865). "Experiments in Plant Hybridization". *↑ Watson, J.D. and F.H. Crick. 1953. ...
"Nucleic Acids Research. 38 (Database issue): D190-5. doi:10.1093/nar/gkp951. PMC 2808932. PMID 19900971.. ... Subsequent hybridisation of those species generates a hybrid genome with a homoeolog copy of each gene from both species. ... "Nucleic Acids Research. 36 (Database issue): D991-8. doi:10.1093/nar/gkm934. PMC 2238940. PMID 17986457.. ... "Nucleic Acids Research. 38 (Database issue): D196-203. doi:10.1093/nar/gkp931. PMC 2808972. PMID 19892828.. ...
16S ribosomal RNA - an intensively studied nucleic acid that has been useful in phylogenetics ... Factors such as mutations, genetic divergence, and hybridization all are considered evolutionary units.[1] ...
sensitive non-radioactive in situ hybridization probes to detect nucleic acids in plants, able to detect 1 µg of plasmid DNA.[6 ... Typically, digoxigenin is introduced chemically (conjugation) into biomolecules (proteins, nucleic acids) to be detected in ... 2002). DIG Application Manual for Nonradioactive in situ Hybridization (3rd ed.). Penzberg: Roche Diagnostics.. ... Their best binder, DIG10.3, was a 141 amino acid protein that bound DIG with a dissociation constant (Kd) of 541 (+/- 193) pM.[ ...
Watson JD, Crick FHC (১৯৫৩)। "[[Molecular structure of Nucleic Acids]]: A Structure for Deoxyribose Nucleic Acid" (PDF)। Nature ... Hybridization (the cross-breeding of genera or species), the cross-breeding of varieties, and general plant breeding। London: ... Hershey AD, Chase M (১৯৫২)। "Independent functions of viral protein and nucleic acid in growth of bacteriophage"। The Journal ... উদ্ধৃতি শৈলী রক্ষণাবেক্ষণ: একাধিক নাম: লেখকগণের তালিকা (link) I. 5. DNA, RNA, and the Flow of Genetic Information: Amino Acids ...
"Nucleic Acids Research. 13 (12): 4401-10. doi:10.1093/nar/13.12.4401. PMC 321795. PMID 2409535.. ... 2012). "Review of techniques and methods in replication and hybridization of transposable elements in vitro". Journal of ... "Nucleic Acids Research. 36 (7): 2284-94. doi:10.1093/nar/gkn064. PMC 2367713. PMID 18287116.. ...
If propionic acid, butyric acid, and longer monocarboxylic acids are produced (see mixed acid fermentation), the amount of ... Strain selection and hybridization developed as well, affecting most modern food fermentations. ... Lactic acid[edit]. Main article: Lactic acid fermentation. Homolactic fermentation (producing only lactic acid) is the simplest ... These lactic acid bacteria can carry out either homolactic fermentation, where the end-product is mostly lactic acid, or ...
"Molecular structure of nucleic acids: A structure for deoxyribose nucleic acid". Nature 171 (4356): 737-738. Bibcode 1953Natur. ... Ang mga hybridization na ito ay artipisyal na isinasagawa sa mga laboratoryo mula 2004 hanggang sa kasaulukuyan. ... Ang DNA, RNA, amino acids, at lipid bilayer na matatagpuan sa lahat ng mga umiiral na organismo ay sumusuporta sa karaniwang ... at mga amino acid ay naingatan sa lahat ng mga alam na buhay. Dahil walang kapakinabangang pantungkulin sa kaliwa o kanang ...
"Nucleic Acids Research. 7 (2): 321-34. doi:10.1093/nar/7.2.321. PMC 328020. PMID 493147.. ... In the laboratory, R-loops may also be created by the hybridization of mature mRNA with double-stranded DNA under conditions ... "Nucleic Acids Research. 6 (7): 2483-97. doi:10.1093/nar/6.7.2483. PMC 327867. PMID 379820.. ... An R-loop is a three-stranded nucleic acid structure, composed of a DNA:RNA hybrid and the associated non-template single- ...
... such as nucleic acids and larger proteins, from inappropriately entering or exiting the nucleus. These large molecules must be ... are stained with fluorescent in situ hybridization. ... from the cytoplasm to the nucleus contain short amino acid ... Mis-expression of a protein due to incomplete excision of exons or mis-incorporation of amino acids could have negative ...
"Nucleic Acid Hybridizations". DNA - Basics of Structure and Analysis. Retrieved 26 May 2017. Beckman, Mary. "Hybridization". ... In molecular biology, hybridization (or hybridisation) is a phenomenon in which single-stranded deoxyribonucleic acid (DNA) or ... Felsenfeld, G; Miles, HT (1967). "The physical and chemical properties of nucleic acids". Annual Review of Biochemistry. 36: ... Fluorescence in situ hybridization (FISH) is a laboratory method used to detect and locate a DNA sequence, often on a ...
Results for nucleic acid hybridization (dna and rna diagnostics) equipment from Analytik Jena and other leading brands. Compare ... nucleic acid hybridization (DNA and RNA Diagnostics) equipment in Canada In Canada Available in Canada Near Canada ... The unit is ideal for Southern, Northern, and Western blots; in situ hybridization; and binding nucleic acids to nitrocellulose ... The UVP Hybrilink Oven portion is ideal for Southern, Northern, and Western blots; in situ hybridization; and binding nucleic ...
Nucleic Acids Res. 2003 Jul 1;31(13):3406-15. Research Support, U.S. Govt, P.H.S. ... Nucleic Acids Res. 2003 Jul 1;31(13):3406-15.. Mfold web server for nucleic acid folding and hybridization prediction.. Zuker M ... for the prediction of the secondary structure of single stranded nucleic acids. The objective of this web server is to provide ... easy access to RNA and DNA folding and hybridization software to the scientific community at large. By making use of ...
No immobilization or labeling of sample nucleic acids is necessary and hybridization can be performed entirely in solution. ... selective for binding the hybrids in the presence of the single stranded sample and probe nucleic acids. ... A nucleic acid hybridization assay employing an immobilized or immobilizable polynucleotide probe selected to form DNA.RNA or ... The state-of-the-art nucleic acid hybridization assay techniques generally involve immobilization of the sample nucleic acid on ...
... and applications of the nucleic acid fragments in the diagnosis of mycobacterial infections are described. More particularly, ... Nucleic acid fragments have been obtained from the genome of mycobacteria, ... Nucleic acid fragments have been obtained from the genome of mycobacteria, and applications of the nucleic acid fragments in ... Nucleic acid fragments have been obtained from the genome of mycobacteria, and applications of the nucleic acid fragments in ...
... for specific nucleic acid sequences can be immobilized as arrays on solid phase surfaces for detection of multiple nucleic acid ... particularly those used for plasmon surface detection and electron transfer detection of nucleic acid. The probes can be washed ... structure between the fluorophore label and a point of attachment to a solid phase is useful as a probe to detect nucleic acid ... A fluorescently labeled nucleic acid having a hairpin ... Immobilized nucleic acid hybridization reagent and method. US ...
Diagnostics based on nucleic acid sequence variant profiling: PCR, hybridization, and NGS approaches.. Khodakov D1, Wang C1, ... Nucleic acid analysis technologies being translated into the clinic can broadly be classified into hybridization, PCR, and ... Nucleic acid sequence variations have been implicated in many diseases, and reliable detection and quantitation of DNA/RNA ...
The methodology is able to detect even a single-copy of a specific nucleic acid of interest under controlled conditions ... or whether the nucleic acid sequence of interest is localized in the chromosomes, nucleus, or cytoplasm of a cell. The methods ... such that a relatively unskilled person can accurately and reproducibly detect even a single-copy of a specific nucleic acid of ... Improved methodologies for in-situ hybridization and non-isotopic detection of nucleic acid sequences are provided which offer ...
Buy the Paperback Book Nucleic Acids Hybridization by Anton Buzdin at Indigo.ca, Canadas largest bookstore. + Get Free ... Nucleic Acids Hybridization: Modern Applications. EditorAnton Buzdin, Sergey Lukyanov. Paperback , October 19, 2010. ... Title:Nucleic Acids Hybridization: Modern ApplicationsFormat:PaperbackDimensions:336 pagesPublished:October 19, 2010Publisher: ... in solution for mutation detection Anton BuzdinCurrent attempts to improve the specificity of nucleic acids hybridization Anton ...
Nucleic acid hybridization has been used in an attempt to determine the fraction of spleen DNA which is homologous to ... Quantitation of Immunoglobulin Genes by Nucleic Acid Hybridization with RNA from Myeloma and Spleen Microsomes. Ursula Storb ... Quantitation of Immunoglobulin Genes by Nucleic Acid Hybridization with RNA from Myeloma and Spleen Microsomes ... Quantitation of Immunoglobulin Genes by Nucleic Acid Hybridization with RNA from Myeloma and Spleen Microsomes ...
Hybridization Biosensors Relying on Electrical Properties of Nucleic Acids. Research output: Contribution to journal/Conference ...
Nucleic Acid Hybridization Nucleic acids Complementary bases Hybridization Complementary strands from any sources Reversible ... Nucleic Acid Hybridization. Description:. Nucleic Acid Hybridization Nucleic acids Complementary bases Hybridization ... Nucleic Acid Hybridization - Nucleic Acid Hybridization. Nucleic acid hybridization is a fundamental tool in ... Benton-Davis. ... Nucleic Acid Hybridization. 1. Nucleic Acid Hybridization. Nucleic acids Complementary bases Hybridization Complementary ...
Enhanced Signals and Fast Nucleic Acid Hybridization By Microfluidic Chaotic Mixing. Angewandte Chemie International Edition, ... Enhanced Signals and Fast Nucleic Acid Hybridization By Microfluidic Chaotic Mixing. Angewandte Chemie International Edition, ... Order from chaos: Microfluidic chaotic mixing shows a significant improvement in the equilibration time of hybridization in DNA ...
Explore Nucleic Acid Detection and Hybridization [Molecular Biology] Categories. Nucleic Acid Detection Substrates and Reagents ... Nucleic Acid Detection and Hybridization [Molecular Biology]. Show Product Brochures Hide Product Brochures *. Chromogenic β- ...
Detection of aquareovirus RNA in fish tissues by nucleic acid hybridization with a cloned cDNA probe.. K Subramanian, B Lupiani ... Detection of aquareovirus RNA in fish tissues by nucleic acid hybridization with a cloned cDNA probe. ... Detection of aquareovirus RNA in fish tissues by nucleic acid hybridization with a cloned cDNA probe. ... Detection of aquareovirus RNA in fish tissues by nucleic acid hybridization with a cloned cDNA probe. ...
... in situ nucleic acid hybridization and an immunocytochemical [alkaline phosphatase-antialkaline phosphatase (APAAP)] technique ... whereas RNA-RNA hybridization is a sensitive technique which can be applied to material stored for years. ... Detection of Yellow Fever Viral RNA by Nucleic Acid Hybridization and Viral Antigen by Immunocytochemistry in Fixed Human Liver ... We therefore compared 2 virus-specific assays applicable to fixed liver, in situ nucleic acid hybridization and an ...
... ... "Diagnostics based on nucleic acid sequence variant profiling: PCR, hybridization, and NGS approaches." Advanced Drug Delivery ... Nucleic acid analysis technologies being translated into the clinic can broadly be classified into hybridization, PCR, and ... Nucleic acid sequence variations have been implicated in many diseases, and reliable detection and quantitation of DNA/RNA ...
Nucleic Acid Hybridization: Principles and Applications (Human Molecular Genetics) as an eTextbook and get instant access. ... Chapter 07 - Nucleic Acid Hybridization: Principles and Applications (Human Molecular Genetics) by Tom Strachan, Andrew Read ... Chapter 07 - Nucleic Acid Hybridization: Principles and Applications (Human Molecular Genetics) 4th Edition by Tom Strachan, ...
Arrays of nucleic acids are formed by techniques, preferably high resolution, light-directe ... Devices and techniques for hybridization of nucleic acids and for determining the sequence of nucleic acids. ... Devices and techniques for hybridization of nucleic acids and for determining the sequence of nucleic acids. Arrays of nucleic ... target nucleic acid is determined by hybridization to an array of nucleic acid probes on a substrate. Each probe is located at ...
The present invention relates to the detection of a target nucleic acid sequence using a target hybridization and detection ... The term used herein "target nucleic acid", "target nucleic acid sequence" or "target sequence" refers to a nucleic acid ... the target nucleic acid sequence is a pre-amplified nucleic acid sequence. The utilization of the pre-amplified nucleic acid ... hybridization of the primer with the target nucleic acid sequence and incubation with the template-dependent nucleic acid ...
plasmid RSF2124 nucleic acids; bacteriophage φX174 nucleic acids; Escherichia coli nucleic acids. ... Studies on Nucleic Acid Reassociation Kinetics: Retarded Rate of Hybridization of RNA with Excess DNA ... Studies on Nucleic Acid Reassociation Kinetics: Retarded Rate of Hybridization of RNA with Excess DNA. Proceedings of the ... We gratefully acknowledge the gifts of φX174 nucleic acids by Drs. Lloyd H. Smith, Amy S. Lee, Paul H. Johnson, and Robert L. ...
Toward an on-chip multiplexed nucleic acid hybridization assay using immobilized quantum dot-oligonucleotide conjugates and ... Target nucleic acid sequences hybridized with QD-probe conjugates and were labeled with Cy3 or Alexa Fluor 647 as acceptor dyes ... A new strategy is presented for the development of multiplexed DNA hybridization assays using immobilized QDs in a microfluidic ...
Implications for DNA hybridization. Einert, T R and Orland, H and Netz, R R - 2011. ... Secondary structure formation of homopolymeric single-stranded nucleic acids including force and loop entropy: Implications for ... Secondary structure formation of homopolymeric single-stranded nucleic acids including force and loop entropy: Implications for ... Secondary structure formation of homopolymeric single-stranded nucleic acids including force and loop entropy: ...
Broder, Graham Richard (2011) The adaption of an encoded microparticle array for multiplexing nucleic acid hybridisation assays ... The adaption of an encoded microparticle array for multiplexing nucleic acid hybridisation assays ... The adaption of an encoded microparticle array for multiplexing nucleic acid hybridisation assays ... The adaption of an encoded microparticle array for multiplexing nucleic acid hybridisation assays ...
Nucleic acid hybridization was used for detection of adenovirus DNA directly in clinical specimens and enterovirus RNA in ... Stool Specimen Hybridization Assay Nitrocellulose Filter Viral Nucleic Acid Hybridization Test These keywords were added by ... Nucleic acid hybridization was used for detection of adenovirus DNA directly in clinical specimens and enterovirus RNA in ... Nucleic acid spot hybridization: rapid quantitative screening of lymphoid cell lines for Epstein-Barr viral DNA. Proc. Natl. ...
The peptide nucleic acid fluorescent in situ hybridization (PNA FISH) test for Enterococcus faecalis and other enterococci ( ... Peptide Nucleic Acid Fluorescent In Situ Hybridization for Hospital-Acquired Enterococcal Bacteremia: Delivering Earlier ... Peptide Nucleic Acid Fluorescent In Situ Hybridization for Hospital-Acquired Enterococcal Bacteremia: Delivering Earlier ... Peptide Nucleic Acid Fluorescent In Situ Hybridization for Hospital-Acquired Enterococcal Bacteremia: Delivering Earlier ...
Hybridization, Dna Sequencing And Other Technologies.Covering: Roche Diagnostics, Abbott - Market research report and industry ... Markets Covered: Polymerase Chain Reaction, Microarrays, Isothermal Nucleic Acid Amplification Technology, Hybridization, Dna ... Isothermal Nucleic Acid Amplification Technology, Hybridization, Dna Sequencing And Other Technologies.Covering: Roche ... Isothermal Nucleic Acid Amplification Technology, Hybridization, Dna Sequencing And Other Technologies.Covering: Roche ...
In Situ Hybridization Detection of Low Copy Nucleic Acid Sequences Using Catalyzed Reporter Deposition and Its Usefulness in ... To determine the sensitivity of CARD-ISH to detect nucleic acids in routinely processed specimens, we analyzed the detection of ... In situ hybridization (ISH) detection of low copy DNA and RNA sequences using nonisotopic probes has been difficult in the past ... the signal-generating potential of labeled hybridized probes and allows the detection of low copy sequences of nucleic acids in ...
  • Probes for specific nucleic acid sequences can be immobilized as arrays on solid phase surfaces for detection of multiple nucleic acid sequences simultaneously from electrophoresis gels and from aqueous solutions. (google.es)
  • These probes and methods for their use can be combined with known solid phases, particularly those used for plasmon surface detection and electron transfer detection of nucleic acid. (google.es)
  • In situ hybridization (ISH) detection of low copy DNA and RNA sequences using nonisotopic probes has been difficult in the past because of a lack of sensitivity. (ovid.com)
  • Catalyzed reporter deposition (CARD) combined with ISH (CARD-ISH) increases the signal-generating potential of labeled hybridized probes and allows the detection of low copy sequences of nucleic acids in formalin-fixed, paraffin-embedded tissue sections. (ovid.com)
  • A new class of homogeneous nucleic acid probes based on specific displacement hybridization. (semanticscholar.org)
  • We have developed a new class of probes for homogeneous nucleic acid detection based on the proposed displacement hybridization. (semanticscholar.org)
  • The probes on their own are quenched, but they become fluorescent upon displacement hybridization with the target. (semanticscholar.org)
  • Cyclicons' as hybridization-based fluorescent primer-probes: synthesis, properties and application in real-time PCR. (semanticscholar.org)
  • Homogeneous amplification and variant detection by fluorescent hybridization probes. (semanticscholar.org)
  • NEW YORK, NY, August 5, 2019 -- Enzo Biochem, Inc. (NYSE: ENZ), an integrated diagnostics and therapeutics company, today announced the issuance of U.S. Patent No. 10,323,272 entitled Nucleic Acid Probes for In Situ Hybridization. (enzo.com)
  • Fluorescence in situ Hybridization method using Peptide Nucleic Acid probes for rapid detection of Lactobacillus and Gardnerella spp. (biomedcentral.com)
  • However, as the application of nucleic acid hybridization for diagnostic and epidemiological purposes becomes almost unavoidable, the logistic problems of keeping numerous individually labeled hybridization probes increase considerably and may reach prohibitory levels in less well-equipped laboratories. (uni-regensburg.de)
  • Peptide nucleic acids can be used in many of the same hybridization applications as natural or synthetic DNA probes but with the added advantages of tighter binding and higher specificity. (barnardhealth.us)
  • 15] Labeled PNAs are then used as probes, allowing hybridization to a denatured dsDNA sample at low ionic strength before loading on the gel. (barnardhealth.us)
  • Because of their neutral backbone, PNA probes present in situ high specificity and require low concentrations and short hybridization time. (barnardhealth.us)
  • However, the hybridization timing of PNA probes (i.e., 45 min) appeared to be significantly shortened in comparison with FISH reaction on sperm. (barnardhealth.us)
  • In this study, we demonstrate remarkable improvement in in situ hybridization efficiency by applying locked-nucleic-acid (LNA)-incorporated oligodeoxynucleotide probes (LNA/DNA probes) without compromising specificity. (elsevier.com)
  • These results suggest that the introduction of LNA residues in DNA probes will be a useful approach for effectively enhancing probe hybridization efficiency. (elsevier.com)
  • The potential of a solution-based hybridization assay using peptide nucleic acid (PNA) molecular beacon (MB) probes to quantify 16S rRNA of specific populations in RNA extracts of environmental samples was evaluated by designing PNA MB probes for the genera Dechloromonas and Dechlorosoma. (nebraska.edu)
  • 8. The LOC device according to claim 7 further comprising an array of the probes, the probes being fluorescence resonance energy transfer (FRET) probes and an array of hybridization chambers containing different types of the FRET probes configured for hybridization with different target nucleic acid sequences, and an array of the photodiodes such that each of the hybridization chambers has a respective one of the photodiodes. (patentsencyclopedia.com)
  • 9. The LOC device according to claim 8 further comprising a polymerase chain reaction (PCR) section for amplifying the target nucleic acid sequences in the fluid prior to hybridization with the FRET probes. (patentsencyclopedia.com)
  • 11. The LOC device according to claim 8 further comprising bond-pads for electrical connection to an external device wherein the CMOS circuitry is configured to convert output from the photodiodes into a signal indicative of the FRET probes that hybridized with the target nucleic acid sequences, and provide the signal to the bond-pads for transmission to the external device. (patentsencyclopedia.com)
  • To compare the efficiency of hybridization methods for the detection of HPV genome, 22 cases of invasive squamous cell carcinoma of the uterine cervix were analyzed by Southern blot analysis and in situ hybridization carried out with 35S- and biotin-labeled probes. (biomedsearch.com)
  • Results showed that in situ hybridization performed with isotopic probes is as sensitive as Southern blot analysis and is more sensitive than that performed with biotin-labeled probe. (biomedsearch.com)
  • Preparation of nucleic acid probes. (springer.com)
  • Detection of enterotoxigenic Escherichia coli by dot blot hybridization with biotinylated DNA probes. (springer.com)
  • A molecular hybridization technique with radiolabeled, strand-specific RNA probes was developed to detect dengue virus type 2 RNA in pools of infected Aedes albopictus mosquitoes. (meta.org)
  • Dot hybridisation with 32 P- and biotin-labelled probes prepared from leptospiral DNA was performed to develop a sensitive and specific diagnostic method for early infection with leptospires. (microbiologyresearch.org)
  • Garrett, KL, Grounds, MD & Beilharz, M 1992, ' Nonspecific binding of nucleic acid probes to Paneth cells in the gastrointestinal tract with in situ hybridization ', Journal of Histochemistry and Cytochemistry , vol. 40, pp. 1613-1618. (edu.au)
  • Our protocol includes details on procedures to isolate mitotic chromosomes from adherent cell cultures, FISH using peptide nucleic acid (PNA) probes on chromosomes in suspension and flow cytometry setup and data acquisition. (scienceexchange.com)
  • Peptide nucleic acid (PNA) probes are artificial DNA analogues with a hydrophobic nature that can penetrate the mycobacterial cell wall . (bvsalud.org)
  • Hybridization probes used in DNA microarrays refer to DNA covalently attached to an inert surface, such as coated glass slides or gene chips, to which a mobile cDNA target is hybridized. (wikipedia.org)
  • Molecular DNA- or RNA-based probes are now routinely used in screening gene libraries, detecting nucleotide sequences with blotting methods, and in other gene technologies, such as nucleic acid and tissue microarrays. (wikipedia.org)
  • Scorpion® probes Molecular Beacon probes TaqMan® probes LNA® (Locked Nucleic Acid) probes Cycling Probe Technology (CPT) Within the field of microbial ecology, oligonucleotide probes are used in order to determine the presence of microbial species, genera, or microorganisms classified on a more broad level, such as bacteria, archaea, and eukaryotes via fluorescence in situ hybridization (FISH). (wikipedia.org)
  • In forensic science, hybridization probes are used, for example, for detection of short tandem repeats (microsatellite) regions and in restriction fragment length polymorphism (RFLP) methods, all of which are widely used as part of DNA profiling analysis. (wikipedia.org)
  • Improved methodologies for in-situ hybridization and non-isotopic detection of nucleic acid sequences are provided which offer major increases of resolution, sensitivity, and simplicity unavailable in previously known techniques. (google.com)
  • We therefore compared 2 virus-specific assays applicable to fixed liver, in situ nucleic acid hybridization and an immunocytochemical [alkaline phosphatase-antialkaline phosphatase (APAAP)] technique. (ajtmh.org)
  • The peptide nucleic acid fluorescent in situ hybridization (PNA FISH) test for Enterococcus faecalis and other enterococci (EFOE) is a multicolor probe that differentiates E. faecalis from other enterococcal species within 3 h directly from blood cultures demonstrating gram-positive cocci in pairs and chains (GPCPC). (asm.org)
  • With its superior sensitivity, simplicity of manufacturing and use, and superb performance in combination with Enzo's PolyView® line of detection reagents, we believe this new probe design will further drive Enzo's business in existing in situ hybridization markets, such as HPV testing. (enzo.com)
  • Dr. Rabbani continued: "Because of its high-sensitivity signal amplification feature, we are also exploring non-in situ uses of this new probe design for the direct detection and quantification of nucleic acids of interest, including very low quantity targets where previously only nucleic acid amplification based techniques that copy the target, such as the Polymerase-mediated Chain Reaction (PCR), were practical. (enzo.com)
  • Fluorescence in situ hybridization (FISH) is a laboratory method used to detect and locate a DNA sequence, often on a particular chromosome. (wikipedia.org)
  • In the 1960s, researchers Joseph Gall and Mary Lou Pardue found that molecular hybridization could be used to identify the position of DNA sequences in situ (i.e., in their natural positions within a chromosome). (wikipedia.org)
  • Since those original observations, many refinements have increased the versatility and sensitivity of the procedure to the extent that in situ hybridization is now considered an essential tool in cytogenetics. (wikipedia.org)
  • The data were obtained by an in situ hybridization technique adapted for use with fixed, plastic-embedded materials. (uni-regensburg.de)
  • In situ hybridization is a method for detecting specific nucleic acid sequences within individual cells. (elsevier.com)
  • In situ hybridization is based on the complementary binding of a labeled nucleic acid probe to complementary sequences in cells or tissue sections, followed by visualization of target sequences within the cells. (elsevier.com)
  • This review will define the technical approaches of in situ hybridization and its current application to detect viral nucleic acids within formalin-fixed, paraffin-embedded tissue samples, with special reference to the Epstein-Barr virus. (elsevier.com)
  • Mabruk, MJEMF 2004, ' In situ hybridization: Detecting viral nucleic acid in formalin-fixed, paraffin-embedded tissue samples ', Expert Review of Molecular Diagnostics , vol. 4, no. 5, pp. 653-661. (elsevier.com)
  • Low signal intensity due to poor probe hybridization efficiency is one of the major drawbacks of rRNA-targeted in situ hybridization. (elsevier.com)
  • In this study, we present the chromosomal imbalances detected in a series of 16 chordomas (10 sacrococcyeal, five sphenooccipital, and one spinal) from 13 patients using comparative genomic hybridization (CGH) and fluorescence in situ hybridization (FISH). (nih.gov)
  • Distribution of type II collagen mRNA in Xenopus embryos visualized by whole-mount in situ hybridization. (biomedsearch.com)
  • Comparison of Southern blot analysis with isotopic and nonisotopic in situ hybridization for the detection of human papillomavirus sequences in invasive carcinoma of the uterine cervix. (biomedsearch.com)
  • Therefore, we quantified adherence of G. vaginalis and other BV-associated bacteria to an inert surface pre-coated with Lactobacillus crispatus using a new Peptide Nucleic Acid (PNA) Fluorescence In Situ Hybridization (FISH) methodology. (mdpi.com)
  • Our goal was to develop and optimize a novel Peptide Nucleic Acid (PNA) Fluorescence in situ Hybridization assay (PNA FISH) for the detection of Lactobacillus spp. (biomedcentral.com)
  • Fluorescence in situ Hybridization (FISH) is a molecular method used to identify and quantify microorganisms in a wide range of samples. (biomedcentral.com)
  • This protocol describes a method developed in our laboratory to analyze repetitive DNA in chromosomes using fluorescence in situ hybridization (FISH) and flow cytometry (chromosome flow FISH or CFF). (scienceexchange.com)
  • A nucleic acid hybridization assay employing an immobilized or immobilizable polynucleotide probe selected to form DNA.RNA or RNA.RNA hybrids with the particular polynucleotide sequence to be determined. (google.ca)
  • This invention relates to nucleic acid hybridization assay methods and reagent systems for detecting specific polynucleotide sequences. (google.ca)
  • The work herein documents the adaption of this technology for the multiplexed analysis of DNA samples in the form of a suspension/hybridisation assay, a design which may offer advantages over current analysis technologies including reduced assay time and increased array flexibility. (soton.ac.uk)
  • Cells infected with coxsackieviruses A and B, echo and polioviruses gave positive signals in the hybridization assay, whereas cells infected with other viruses did not. (springer.com)
  • A dot hybridization assay for detection of rotavirus. (springer.com)
  • The detection limit of the PNA MB hybridization assay was determined to be 1.6 nM of 16S rRNA. (nebraska.edu)
  • To establish proof for the application of PNA MB hybridization assays in complex systems, target 16S rRNA from Dechlorosoma suillum was spiked at different levels to RNA isolated from an environmental (bioreactor) sample, and the PNA MB assay enabled effective quantification of the D. suillum RNA in this complex mixture. (nebraska.edu)
  • For another environmental sample, the quantitative results from the PNA MB hybridization assay were compared with those from clone libraries. (nebraska.edu)
  • Li, X , Morgenroth, E & Raskin, L 2008, ' Quantitative rRNA-targeted solution-based hybridization assay using peptide nucleic acid molecular beacons ', Applied and environmental microbiology , vol. 74, no. 23, pp. 7297-7305. (nebraska.edu)
  • A nucleic acid hybridization assay was assembled onto a robust and readily addressable silicon-based chip using polysaccharide chitosan as a scaffold for the covalent coupling of probe DNA to the chip's surface. (umd.edu)
  • Finally, transcriptional upregulation of the Escherichia coli chaperone, DnaK, which is an indicator of cellular stress, was observed using the hybridization chip sandwich assay. (umd.edu)
  • The principle of nucleic acid hybridization assays was developed by workers in the recombinant DNA field as a means for determining and isolating particular polynucleotide base sequences of interest. (google.ca)
  • A new strategy is presented for the development of multiplexed DNA hybridization assays using immobilized QDs in a microfluidic system. (spie.org)
  • abstract = "Selection and adjustment of proper physical parameters enables rapid DNA transport, site selective concentration, and accelerated hybridization reactions to be carried out on active microelectronic arrays. (elsevier.com)
  • abstract = "Continuous culture of Pseudomonas stutzeri Zobell, a marine denitrifying bacterium, was used to determine the relationship between growth rate and nucleic acid content. (princeton.edu)
  • Amino acids are added to the growing protein chain and are held together by peptide bonds. (reference.com)
  • To the N-terminus of a nonamer peptide nucleic acid sequence, H-GCACGACTT-NH2, was attached a number of lipophilic conjugate molecules including three synthetic tocopherol (vitamin E) analogues. (edu.au)
  • The binding of a mixed-sequence pentadecamer PNA (peptide nucleic acid) containing all four nucleobases to the fully complementary as well as various singly mismatched RNA and DNA oligonucleotides has been systematically investigated using thermal denaturation and BIAcore surface-interaction techniques. (chalmers.se)
  • The abbreviated name, 'mfold web server', describes a number of closely related software applications available on the World Wide Web (WWW) for the prediction of the secondary structure of single stranded nucleic acids. (nih.gov)
  • Resulting hybrids are detected by binding of an antibody reagent, preferably labeled with a detectable chemical group, selective for binding the hybrids in the presence of the single stranded sample and probe nucleic acids. (google.ca)
  • It was found that single stranded nucleic acids, e.g. (google.ca)
  • DNA and RNA, such as obtained by denaturing their double stranded forms, will hybridize or recombine under appropriate conditions with complementary single stranded nucleic acids. (google.ca)
  • By labeling such complementary probe nucleic acids with some readily detectable chemical group, it was then made possible to detect the presence of any polynucleotide sequence of interest in a test medium containing sample nucleic acids in single stranded form. (google.ca)
  • In molecular biology, hybridization (or hybridisation) is a phenomenon in which single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) molecules anneal to complementary DNA or RNA. (wikipedia.org)
  • Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. (lexic.us)
  • Likewise, hybridization PNA-based biosensor procedures have been developed in which a single-stranded PNA probe is immobilized onto optical or mass-sensitive transducers to detect the complementary strand or corresponding mismatch in a DNA sample solution. (barnardhealth.us)
  • Hybridisation is a term used to describe the specific complementary association due to hydrogen bonding, under experimental conditions, of single-stranded nucleic acids. (uct.ac.za)
  • One may make ones nucleic acid single-stranded for the purpose of annealing - if it is not single-stranded already, like most RNA viruses - by heating it in 0.01M NaCl to a point above the 'melting temperature' of the double- or partially-double-stranded form, and then flash-cooling to + 0oC: this ensures the 'denatured' or separated strands do not re-anneal. (uct.ac.za)
  • One immobilises 'target' nucleic acid - denatured so as to be effectively single-stranded - on an absorptive, porous membrane, and then anneals to it an appropriately 'tagged' or 'labelled' single-stranded probe nucleic acid. (uct.ac.za)
  • The probe thereby hybridizes to single-stranded nucleic acid (DNA or RNA) whose base sequence allows probe-target base pairing due to complementarity between the probe and target. (wikipedia.org)
  • Diagnostics based on nucleic acid sequence variant profiling: PCR, hybridization, and NGS approaches. (nih.gov)
  • Nucleic acid sequence variations have been implicated in many diseases, and reliable detection and quantitation of DNA/RNA biomarkers can inform effective therapeutic action, enabling precision medicine. (nih.gov)
  • or whether the nucleic acid sequence of interest is localized in the chromosomes, nucleus, or cytoplasm of a cell. (google.com)
  • Devices and techniques for hybridization of nucleic acids and for determining the sequence of nucleic acids. (patents.com)
  • Devices and techniques are proposed to determine the sequence of a target nucleic acid more efficiently and more quickly through such synthesis and detection techniques. (patents.com)
  • Nick Translation Nick Translation: Involves the synthesis and labeling of a Probe A piece of Nucleic Acid labeled (incorporated) with a Tracer that allows one to 'track' the hybridization of the Probe to an unknown sequence of DNA A Tracer could be: 1. (coursehero.com)
  • In an effort to provide an attractive alternative to conventional methods, which are typically unsuitable for routine scenarios, this work builds on a unique, bead-based technique for sequence-specific DNA detection known as Hybridization-Induced Aggregation (HIA). (virginia.edu)
  • Thus, the association of two nucleic acid molecules - presumed to be at least a few hundred bases long - is an extremely sequence-specific process, far more so than the widely-used specificity of monoclonal antibodies in binding to specific antigenic determinants. (uct.ac.za)
  • BDA is compatible with inexpensive thermocycler instrumentation and employs a rationally designed competitive hybridization reaction to achieve comparable enrichment performance across annealing temperatures ranging from 56 °C to 64 °C. To show the sequence generality of BDA, we demonstrate enrichment of 156 SNVs and the reliable detection of single-digit copies. (nature.com)
  • 7. The LOC device according to claim 1 wherein the primer-linked, linear probe further comprises a fluorophore and a quencher such that the quencher is removed when the nucleic acid sequence matching the target nucleic acid sequence anneals to the complementary sequence. (patentsencyclopedia.com)
  • 1. An isolated and substantially pure polypeptide, comprising a polypeptide having a purity of greater than 75% by weight and a molecular weight of about 700 to about 4000 daltons and having the amino acid sequence Gln-Gln-Ser-Tyr-Ser-Ile-Met-Ser-Ile-Ile-Lys-Glu-Glu-Val-Leu-Ala-Tyr-Val-Val-Gln-Leu-Pro-Leu-Tyr-Gly-Val-Ile-Asp-Thr-Pro-Cys-Trp-Lys. (google.co.uk)
  • 3. An isolated and substantially pure polypeptide, comprising a polypeptide having a purity of greater than 75% by weight and a molecular weight between about 700 to about 2500 daltons and having the amino acid sequence Glu-Glu-Val-Leu-Ala-Tyr-Val. (google.co.uk)
  • Objective To establish a method of gold nanoprobe-based solution hybridization (GNBSH) to detect nucleic acid sequence-based amplification ( NASBA ) products for the rapid diagnosis of invasive aspergillosis (IA). (bvsalud.org)
  • In this case, the slowdown of DNA during electrophoretic passage is related to its hybridization with a complementary PNA probe entrapped in the gel matrix. (barnardhealth.us)
  • Figure 9.6 Nucleic Acid Hybridization Can Be Used to Locate Homologous Sequences perature is lowered and the pH is neutral, the two strands will anneal because of the base-pairing interactions of the complementary strands. (alpfmedical.info)
  • Two complementary strands from different sources will also anneal, but the process may then be called hybridization to reflect the fact that each strand originated from a different source to create a hybrid molecule. (alpfmedical.info)
  • DNA replication and transcription of DNA into RNA both rely upon nucleotide hybridization, as do molecular biology techniques including Southern blots and Northern blots, the polymerase chain reaction (PCR), and most approaches to DNA sequencing. (wikipedia.org)
  • A variety of different methods use hybridization to pinpoint the origin of a DNA sample, including the polymerase chain reaction (PCR). (wikipedia.org)
  • Combining nucleic acid amplification and detection. (semanticscholar.org)
  • Significantly, the new probe design permits the detection of such targets without the disadvantages encompassed in competing high-sensitivity methods such as nucleic acid amplification-based detection and branched DNA (bDNA) probe technologies, which can involve high cost, high complexity, time consuming processes and disruptions of sample integrity. (enzo.com)
  • The performance of nucleic acid amplification tests (NAATs) with respect to overall sensitivity, specificity, and ease of specimen transport is better than that of any of the other tests available for the diagnosis of chlamydial and gonococcal infections. (cdc.gov)
  • The present invention relates to nucleic acid fragments derived from the genome of an appropriate mycobacterium, in particular Mycobacterium tuberculosis, to their applications in the diagnosis of mycobacterial infections, and to plasmids containing the said fragments. (google.com)
  • Novel test for rapid viral diagnosis: Detection of adenovirus in nasopharyngeal mucus aspirates by means of nucleic-acid sandwich hybridization. (springer.com)
  • In a new sandwich technique, the first step involves hybridization with an unlabeled recombinant m 13 DNA carrying an insert of the desired specificity. (uni-regensburg.de)
  • The effects of probe-target identity, continuous stretch, mismatch position, and hybridization free energy on specificity were tested. (psu.edu)
  • The sensitivity and accuracy of the two methods are compared, and the potential for species specificity in future hybridizations is discussed. (princeton.edu)
  • No immobilization or labeling of sample nucleic acids is necessary and hybridization can be performed entirely in solution. (google.ca)
  • 2. The method for analyzing nucleic acid mutation according to claim 1, wherein at least two kinds among n kinds of the sample nucleic acids are analyte nucleic acids. (patentsencyclopedia.com)
  • 4. The method for analyzing nucleic acid mutation according to claim 1, wherein at least one kind among n kinds of the sample nucleic acids is a standard nucleic acid. (patentsencyclopedia.com)
  • 6. The method for analyzing nucleic acid mutation according to claim 1, wherein n kinds of the sample nucleic acids are nucleic acids derived from different individuals belonging to taxonomically the same species. (patentsencyclopedia.com)
  • 8. The method for analyzing nucleic acid mutation according to claim 1, wherein the sample nucleic acids are all labeled with the same label. (patentsencyclopedia.com)
  • Nucleic acid spot hybridization: rapid quantitative screening of lymphoid cell lines for Epstein-Barr viral DNA. (springer.com)
  • Hyypia T., Stalhandske P., Vainionpaa R., Halonen P., Pettersson U. (1984) Detection of Viral Nucleic Acids in Cell Cultures and in Clinical Specimens by Spot Hybridization. (springer.com)
  • This technique permits visualization of viral nucleic acid or gene expression in individual cells within their histologic context. (elsevier.com)
  • It has been used widely for the detection of viral nucleic acid sequences within individual cells. (elsevier.com)
  • Nucleic acid hybridization has been used to detect viral nucleic acid in water. (elsevier.com)
  • Analyzing and Comparing Nucleic Acid Sequences by Hybridization to Arrays of Oligonucleotides: Evaluation Using Experimental Models," Genomics (1992) 13:1008-1017. (patents.com)
  • Reversible DNA hybridization can be used as a new mechanism to control the sustained and triggered release of therapeutic oligonucleotides from hydrogels. (elsevier.com)
  • Order from chaos: Microfluidic chaotic mixing shows a significant improvement in the equilibration time of hybridization in DNA microarrays over conventional techniques. (caltech.edu)
  • The fast hybridization kinetics of PNA was similar to the kinetics of PRINS reaction. (barnardhealth.us)
  • Chapter 07 - Nucleic Acid Hybridization: Principles and Applications (Human Molecular Genetics) 4th Edition by Tom Strachan, Andrew Read and Publisher Garland Science. (vitalsource.com)
  • A fluorescently labeled nucleic acid having a hairpin structure between the fluorophore label and a point of attachment to a solid phase is useful as a probe to detect nucleic acid from a sample. (google.es)
  • Nucleic acid fragments have been obtained from the genome of mycobacteria, and applications of the nucleic acid fragments in the diagnosis of mycobacterial infections are described. (google.com)
  • 7. The method according to claim 1, wherein the target nucleic acid is a deoxyribonucleic acid. (google.com)
  • Labelling deoxyribonucleic acid to high specific activity by nick translation with DNA-polymerase I. J. Mol. (springer.com)
  • In 1962 James Watson (b. 1928), Francis Crick (1916-2004), and Maurice Wilkins (1916-2004) jointly received the Nobel Prize in physiology or medicine for their 1953 determination of the structure of deoxyribonucleic acid (DNA). (wikipedia.org)
  • The control of ribonucleic acid synthesis in bacteria. (portlandpress.com)
  • Changes in the cell content and rate of synthesis of mRNA were studied in auxotrophs of Escherichia coli recovering from a period of amino acid deprivation. (portlandpress.com)
  • The complexity of the secondary structures makes RNA targets inaccessible for probe hybridization, resulting in decreased sensitivity and selectivity. (usda.gov)
  • Upon target-probe hybridization, the beads become tethered together, resulting in optically-detectable bead aggregation. (virginia.edu)
  • In contrast, buffers such as glycine, β-alanine and γ-amino-butyric acid (GABA) produce rapid transport and site selective concentration but do not facilitate hybridization. (elsevier.com)
  • Hybridization temperatures and formamide concentrations in hybridization buffers were optimized to minimize signals from hybridizations of PNA MB to nontarget 16S rRNAs. (nebraska.edu)
  • Detection of sequences with moderate or high similarity depends on how stringent the hybridization conditions were applied-high stringency, such as high hybridization temperature and low salt in hybridization buffers, permits only hybridization between nucleic acid sequences that are highly similar, whereas low stringency, such as lower temperature and high salt, allows hybridization when the sequences are less similar. (wikipedia.org)
  • There is provided a method for analyzing nucleic acid mutation using an array comparative genomic hybridization technique, which reduces the false positive and the false negative and improves the reliability of the analysis results. (patentsencyclopedia.com)
  • 9. The method for analyzing nucleic acid mutation according to claim 1, wherein the label intensities F1 to Fn in the step (b) are corrected values. (patentsencyclopedia.com)
  • 10. A method according to claim 8 , wherein said oligonucleotide is indirectly linked to said solid phase by hybridization with said nucleic acid. (google.es)
  • Chetverin, A.B. and Kramer, F.R., "Novel Oligonucleotide Arrays and Their Use for Sorting, Isolating, Sequencing and Manipulating Nucleic Acids," pp. 1-67 and attached Figures (publication journal title and date unknown). (patents.com)
  • Elder, "Analysis of DNA Oligonucleotide Hybridization Data by Maximum Entropy," Maximum Entropy and Bayesian Methods Paris, 1992, pp. 1-10. (patents.com)
  • An oligonucleotide hybridization approach to DNA sequencing, FEBS Letters, 256:118-122 (1989). (patents.com)
  • The clones are of insufficient sensitivity to detect infection-specific RNA in dot and northern blots of crude nucleic acid extracts. (herts.ac.uk)
  • Ribonucleic acid, known as RNA, is composed of a combination of four different nucleotides: adenine, uracil, guanine and cytosine. (reference.com)
  • The three types of ribonucleic acid are messenger RNA, ribosomal RNA and transfer RNA. (reference.com)
  • Detection of aquareovirus RNA in fish tissues by nucleic acid hybridization with a cloned cDNA probe. (asm.org)
  • Nucleic acid analysis technologies being translated into the clinic can broadly be classified into hybridization, PCR, and sequencing, as well as their combinations. (nih.gov)
  • An alternative to Southern analysis is also the PNA pregel hybridization process, which significantly simplifies the procedure of Southern hybridization. (barnardhealth.us)
  • The practical translation of these findings into our mainstream medical regime requires nucleic acid analysis technology that is affordable, time-efficient, and simple to execute. (virginia.edu)
  • Overall, this work represents practical steps forward in the development of nucleic acid analysis technology that is amenable for routine clinical care, in order to ultimately reap the rewards of a personalized medical regime. (virginia.edu)
  • Here, we show that, through electric-field-induced reconfiguration of a network of gold-coated magnetic nanoparticles modified by probe DNA ([email protected]), it is possible to create a highly sensitive sensor for direct analysis of nucleic acids in samples as complex as whole blood. (crcm-marseille.fr)
  • There are two major factors affecting the hybridization efficiency: probe accessibility and affinity to the targeted rRNA molecules. (elsevier.com)
  • In a kinetic study with 16S rRNA from pure cultures, the hybridization of PNA MB to target 16S rRNA exhibited a higher final hybridization signal and a lower apparent rate constant than the hybridizations to nontarget 16S rRNAs. (nebraska.edu)
  • We also developed a 200-bp nucleic acid probe (with species- specific potential) for a portion of the 23S rRNA gene of P. stutzeri Zobell, which was used to quantify rRNA and rDNA by hybridization in the same continuous cultures. (princeton.edu)
  • The turnover rate for newly formed mRNA and rRNA was virtually the same in "stepped-down" rel+ and rel- strains and was similar to that of the same fraction in amino acid-starved rel+ cells. (portlandpress.com)
  • The global standard library of rRNA sequences is constantly becoming larger and continuously being updated, and thus the possibility of a random hybridization event between a specifically-designed probe (based on complete and current data from a range of test organisms) and an undesired/unknown target organism cannot be easily dismissed. (wikipedia.org)
  • The protein product of FDH shows similarity to condensing enzymes involved in lipid biosynthesis, particularly those of the FATTY ACID ELONGATION family. (plantcell.org)
  • Identification of DNA viruses by membrane filter hybridization. (springer.com)
  • 8.) Add your 100 microlitre of the boiled probe DNA hybridization mixture to your nylon membrane (DNA side up still). (coursehero.com)
  • The answer is simple: nucleic acid hybridisation on membrane filters is a simple, sensitive, and specific means of detecting nucleic acid sequences of interest. (uct.ac.za)
  • A concentration of 10 mM NaCl in the hybridization buffer was found to be optimal for maximizing the difference between final hybridization signals from target and nontarget 16S rRNAs. (nebraska.edu)
  • 10. The LOC device according to claim 9 wherein the PCR section and the hybridization chambers are incorporated in a microsystems technology (MST) layer having a plurality of channels configured to draw the fluid through the PCR section and into the hybridization chambers by capillary action. (patentsencyclopedia.com)
  • c) detecting the probe hybridized to said target nucleic acid if present. (google.com)
  • Positions of hybridization of a target nucleic acid are determined by, e.g., epifluorescence microscopy. (patents.com)
  • Target nucleic acid sequences hybridized with QD-probe conjugates and were labeled with Cy3 or Alexa Fluor 647 as acceptor dyes for the QD donors, where FRET-sensitized dye emission provided a signal for the detection of picomolar quantities of target. (spie.org)
  • However, this is accompanied by a decrease in the rate of molecular beacon±target hybridization. (psu.edu)
  • 2. The method of claim 1 wherein before step(b) the resulting immobilized hybridized nucleic acids from the test medium are separated from the remainder of the reaction mixture. (google.ca)
  • Several approaches, such as microarray hybridization, have become extremely popular tools for specialists in biochemistry and biomedicine, while the potential of many other advantageous techniques seems to be underestimated. (indigo.ca)
  • 12. A method according to claim 11 , wherein said array comprises a plurality of different nucleic acids. (google.es)