Antigens, Polyomavirus Transforming
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Antigens, Viral, Tumor
Receptors, Antigen, T-Cell
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
Proliferating Cell Nuclear Antigen
Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.
A group of antigens that includes both the major and minor histocompatibility antigens. The former are genetically determined by the major histocompatibility complex. They determine tissue type for transplantation and cause allograft rejections. The latter are systems of allelic alloantigens that can cause weak transplant rejection.
Histocompatibility Antigens Class II
Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.
The lipopolysaccharide-protein somatic antigens, usually from gram-negative bacteria, important in the serological classification of enteric bacilli. The O-specific chains determine the specificity of the O antigens of a given serotype. O antigens are the immunodominant part of the lipopolysaccharide molecule in the intact bacterial cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Receptors, Antigen, B-Cell
Antigens, Tumor-Associated, Carbohydrate
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Enzyme-Linked Immunosorbent Assay
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Blood Group Antigens
Hepatitis B Surface Antigens
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
Polymorphic class I human histocompatibility (HLA) surface antigens present on almost all nucleated cells. At least 20 antigens have been identified which are encoded by the A locus of multiple alleles on chromosome 6. They serve as targets for T-cell cytolytic responses and are involved with acceptance or rejection of tissue/organ grafts.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Histocompatibility Antigens Class I
Membrane glycoproteins consisting of an alpha subunit and a BETA 2-MICROGLOBULIN beta subunit. In humans, highly polymorphic genes on CHROMOSOME 6 encode the alpha subunits of class I antigens and play an important role in determining the serological specificity of the surface antigen. Class I antigens are found on most nucleated cells and are generally detected by their reactivity with alloantisera. These antigens are recognized during GRAFT REJECTION and restrict cell-mediated lysis of virus-infected cells.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
Human immune-response or Class II antigens found mainly, but not exclusively, on B-lymphocytes and produced from genes of the HLA-D locus. They are extremely polymorphic families of glycopeptides, each consisting of two chains, alpha and beta. This group of antigens includes the -DR, -DQ and -DP designations, of which HLA-DR is most studied; some of these glycoproteins are associated with certain diseases, possibly of immune etiology.
High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.
Hepatitis B Antigens
55-kDa antigens found on HELPER-INDUCER T-LYMPHOCYTES and on a variety of other immune cell types. CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MAJOR HISTOCOMPATIBILITY COMPLEX class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as INTERLEUKIN-15 receptors and bind to the HIV receptors, binding directly to the HIV ENVELOPE PROTEIN GP120.
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Amino Acid Sequence
Class I human histocompatibility (HLA) surface antigens encoded by more than 30 detectable alleles on locus B of the HLA complex, the most polymorphic of all the HLA specificities. Several of these antigens (e.g., HLA-B27, -B7, -B8) are strongly associated with predisposition to rheumatoid and other autoimmune disorders. Like other class I HLA determinants, they are involved in the cellular immune reactivity of cytolytic T lymphocytes.
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CTLA-4 ANTIGEN with high specificity and to CD28 ANTIGEN with low specificity. The interaction of CD80 with CD28 ANTIGEN provides a costimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
Epstein-Barr Virus Nuclear Antigens
Hepatitis B Core Antigens
A member of the tumor necrosis factor receptor superfamily with specificity for CD40 LIGAND. It is found on mature B-LYMPHOCYTES and some EPITHELIAL CELLS, lymphoid DENDRITIC CELLS. Evidence suggests that CD40-dependent activation of B-cells is important for generation of memory B-cells within the germinal centers. Mutations of the gene for CD40 antigen result in HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 3. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
Complement Fixation Tests
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CD28 ANTIGEN with high specificity and to CTLA-4 ANTIGEN with low specificity. The interaction of CD86 with CD28 ANTIGEN provides a stimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
Simian virus 40
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
Receptors, Antigen, T-Cell, alpha-beta
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
Electrophoresis, Polyacrylamide Gel
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
gp100 Melanoma Antigen
Lewis Blood-Group System
A group of dominantly and independently inherited antigens associated with the ABO blood factors. They are glycolipids present in plasma and secretions that may adhere to the erythrocytes. The phenotype Le(b) is the result of the interaction of the Le gene Le(a) with the genes for the ABO blood groups.
Mice, Inbred Strains
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
Sensitivity and Specificity
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
Hepatitis B e Antigens
A tumor necrosis factor receptor subtype found in a variety of tissues and on activated LYMPHOCYTES. It has specificity for FAS LIGAND and plays a role in regulation of peripheral immune responses and APOPTOSIS. Multiple isoforms of the protein exist due to multiple ALTERNATIVE SPLICING. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.
Minor Histocompatibility Antigens
Allelic alloantigens often responsible for weak graft rejection in cases when (major) histocompatibility has been established by standard tests. In the mouse they are coded by more than 500 genes at up to 30 minor histocompatibility loci. The most well-known minor histocompatibility antigen in mammals is the H-Y antigen.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Major Histocompatibility Complex
The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.
Class I human histocompatibility (HLA) antigens encoded by a small cluster of structural genes at the C locus on chromosome 6. They have significantly lower immunogenicity than the HLA-A and -B determinants and are therefore of minor importance in donor/recipient crossmatching. Their primary role is their high-risk association with certain disease manifestations (e.g., spondylarthritis, psoriasis, multiple myeloma).
ABO Blood-Group System
The major human blood type system which depends on the presence or absence of two antigens A and B. Type O occurs when neither A nor B is present and AB when both are present. A and B are genetic factors that determine the presence of enzymes for the synthesis of certain glycoproteins mainly in the red cell membrane.
Tumor Cells, Cultured
Glycoproteins expressed on all mature T-cells, thymocytes, and a subset of mature B-cells. Antibodies specific for CD5 can enhance T-cell receptor-mediated T-cell activation. The B-cell-specific molecule CD72 is a natural ligand for CD5. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
A member of the tumor necrosis factor receptor superfamily found on most T-LYMPHOCYTES. Activation of the receptor by CD70 ANTIGEN results in the increased proliferation of CD4-POSITIVE T-LYMPHOCYTES and CD8-POSITIVE T-LYMPHOCYTES. Signaling by the activated receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
Binding Sites, Antibody
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Substances that augment, stimulate, activate, potentiate, or modulate the immune response at either the cellular or humoral level. The classical agents (Freund's adjuvant, BCG, Corynebacterium parvum, et al.) contain bacterial antigens. Some are endogenous (e.g., histamine, interferon, transfer factor, tuftsin, interleukin-1). Their mode of action is either non-specific, resulting in increased immune responsiveness to a wide variety of antigens, or antigen-specific, i.e., affecting a restricted type of immune response to a narrow group of antigens. The therapeutic efficacy of many biological response modifiers is related to their antigen-specific immunoadjuvanticity.
Dose-Response Relationship, Immunologic
Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.
Recombinant Fusion Proteins
Receptors, Antigen, T-Cell, gamma-delta
T-cell receptors composed of CD3-associated gamma and delta polypeptide chains and expressed primarily in CD4-/CD8- T-cells. The receptors appear to be preferentially located in epithelial sites and probably play a role in the recognition of bacterial antigens. The T-cell receptor gamma/delta chains are separate and not related to the gamma and delta chains which are subunits of CD3 (see ANTIGENS, CD3).
Tumor Markers, Biological
Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445)
Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Herpesvirus 4, Human
A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.
Cytotoxicity Tests, Immunologic
A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.
Genes, MHC Class II
Ovarian follicle activation
It was shown that two proliferation markers, Ki-67 and proliferating cell nuclear antigen (PCNA), are regulated by these ...
Similarly, proliferating cell nuclear antigen (PCNA) is a protein associated with cell proliferation that is upregulated in ... "Assessment of Ki67 in Breast Cancer: Recommendations from the International Ki67 in Breast Cancer Working Group". JNCI Journal ... Ki-67 is a nuclear antigen expressed in proliferating cells that is coded by the MKI67 gene on chromosome 10, and is expressed ... The Ki-67 score closely correlates with other proliferation markers, and has been shown to have prognostic and predictive value ...
List of MeSH codes (D23)
... tissue polypeptide antigen MeSH D23.050.290.249 - epstein-barr virus nuclear antigens MeSH D23.050.290.500 - ki-67 antigen MeSH ... htlv-i antigens MeSH D23.050.327.150.510 - htlv-ii antigens MeSH D23.050.327.300 - epstein-barr virus nuclear antigens MeSH ... antigens, cd15 MeSH D18.104.22.1680.131 - antigens, cd31 MeSH D22.214.171.1240 - antigens, ly MeSH D126.96.36.1990 - antigens, ... forssman antigen MeSH D23.050.285.018 - antigens, cd24 MeSH D23.050.285.025 - antigens, cd30 MeSH D23.050.285.040 - antigens, ...
Gerdes J, Schwab U, Lemke H, Stein H (1983). "Production of a mouse monoclonal antibody reactive with a human nuclear antigen ... Antigen KI-67 also known as Ki-67 or MKI67 (Marker Of Proliferation Ki-67) is a protein that in humans is encoded by the MKI67 ... Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis. The Ki-67 protein (also known as MKI67) is a ... "Entrez Gene: Antigen identified by monoclonal antibody Ki-67". Schonk DM, Kuijpers HJ, van Drunen E, van Dalen CH, Geurts van ...
At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded ... 2002). "The Ki-67 protein interacts with members of the heterochromatin protein 1 (HP1) family: a potential role in the ... This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding ... CBX3 has been shown to interact with PIM1, Ki-67, Lamin B receptor, CBX5 and CBX1. Heterochromatin protein 1 GRCh38: Ensembl ...
Proliferating cell nuclear antigen
Antibodies against proliferating cell nuclear antigen (PCNA) or monoclonal antibody termed Ki-67 can be used for grading of ... Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic ... "Entrez Gene: PCNA proliferating cell nuclear antigen". Leonardi E, Girlando S, Serio G, Mauri FA, Perrone G, Scampini S, Dalla ... Webb G, Parsons P, Chenevix-Trench G (1991). "Localization of the gene for human proliferating nuclear antigen/cyclin by in ...
Glandular odontogenic cyst
Proliferating cell nuclear antigen readings were established to have no role in the differentiation process. The TGF-beta ... Similarly, Ki-67 was seen less in the central MEC compared to the other lesions, though this discovery is not essential to the ... Past studies observed Ki-67, p53, and PCNA expression in common jaw cysts that shared similar characteristics. There was a lack ...
Imai Y, Soda M, Hatakeyama S, Akagi T, Hashikawa T, Nakayama KI, Takahashi R (Jul 2002). "CHIP is associated with Parkin, a ... "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ... "Identification of tumor-associated antigens in chronic lymphocytic leukemia by SEREX". Blood. 100 (6): 2123-31. doi:10.1182/ ... Imai Y, Soda M, Hatakeyama S, Akagi T, Hashikawa T, Nakayama KI, Takahashi R (Jul 2002). "CHIP is associated with Parkin, a ...
"Strong association between IgA nephropathy and HLA-DR4 antigen". Kidney Int. 22 (4): 377-82. doi:10.1038/ki.1982.185. PMID ... an analysis of 622 nuclear families". Tissue Antigens. 62 (2): 162-9. doi:10.1034/j.1399-0039.2003.00071.x. PMID 12889996. ... Kaibe M, Takakuwa K, Murakawa H, Ishii K, Tamura M, Tanaka K (2006). "Studies on the human leukocyte antigens in patients with ... Matsuyama M, Hashimoto K, Yamasaki Y, Shirakura R, Higuchi R, Miyajima T, Amemiya H (1981). "HLA-DR antigens in pemphigus among ...
... cytosolic thymidine kinase as compared to proliferating cell nuclear antigen in patients with colorectal carcinoma". Anticancer ... The highest number of patients identified was obtained by combination of thymidine kinase and Ki-67 staining. The technique has ... immunohistochemical detection of cytosolic thymidine kinase and proliferating cell nuclear antigen in breast cancer". Cancer ... Lee MH, Wang L, Chang ZF (April 2014). "The contribution of mitochondrial thymidylate synthesis in preventing the nuclear ...
Primary testicular diffuse large B-cell lymphoma
They may also express CD10 (30-60% of cases), IRF4 (35-65% of cases), and nuclear BCL6 (60-90% of cases); ~80% of cases are " ... They are rapidly proliferating cells as determined by testing for Ki-67 staining. In at least seventy-five percent of cases, ... Immune privileged sites are sanctuaries where abnormal antigens that occur in them, such as those that are expressed on cancer ... 52 (1): 53-67. doi:10.1016/j.pathol.2019.08.013. PMID 31735345. Twa DD, Mottok A, Savage KJ, Steidl C (May 2018). "The ...
Cluster of Excellence Frankfurt Macromolecular Complexes
CEF scientists evaluated members of the SR protein family (SRSF1-7) for their potential to act as adaptors for nuclear export ... PMID 31732457.CS1 maint: multiple names: authors list (link) Okazaki KI, Wöhlert D, Warnau J, Jung H, Yildiz O, Kühlbrandt W, ... Their NMR data also provided unparalleled insights into the nature of the interactions between the side chains of the antigen ... The conformational coupling and trans-inhibition in the human antigen transporter ortholog TmrAB was resolved with the aid of ...
Primary biliary cholangitis
March 2006). "Increased expression of Nuclear Envelope gp210 Antigen in Small Bile Ducts in Primary Biliary Cirrhosis". Journal ... Mells GF, Floyd JA, Morley KI, et al. (April 2011). "Genome-Wide Association study Identifies 12 New Susceptibility Loci for ... Such autoreactivity may also be the case with other proteins, including the gp210 and p62 nuclear pore proteins. Gp210 has ... Fibric acid derivatives, or fibrates, are agonists of the peroxisome proliferator activator receptor (PPAR), a nuclear receptor ...
... this protein enhances antigen-specific tumor immunity by facilitating more efficient antigen presentation to cytotoxic T cells ... Imai Y, Soda M, Hatakeyama S, Akagi T, Hashikawa T, Nakayama KI, Takahashi R (July 2002). "CHIP is associated with Parkin, a ... nuclear condensation, and fragmentation of the DNA and nucleus. This is followed by fragmentation into apoptotic bodies that ... forming a number of plasma membrane-bounded apoptotic bodies which contain nuclear and or cytoplasmic elements. The ...
Anaplastic lymphoma kinase
"Entrez Gene: ALK anaplastic lymphoma kinase (Ki-1)". Shiota M, Fujimoto J, Semba T, Satoh H, Yamamoto T, Mori S (June 1994). " ... March 2002). "ALK as a novel lymphoma-associated tumor antigen: identification of 2 HLA-A2.1-restricted CD8+ T-cell epitopes". ... August 2003). "Identification and characterization of a nuclear interacting partner of anaplastic lymphoma kinase (NIPA)". The ... Yaakup H, Sagap I, Fadilah SA (October 2008). "Primary oesophageal Ki (CD30)-positive ALK+ anaplastic large cell lymphoma of T- ...
سرطان پروستات - ویکیپدیا، دانشنامهٔ آزاد
Prostate specific membrane antigen is a transmembrane carboxypeptidase and exhibits folate hydrolase activity. This protein ... The expression of Ki-67 by immunohistochemistry may be a significant predictor of patient outcome for men with prostate cancer. ... AR, an androgen-activated transcription factor, belongs to the steroid nuclear receptor family. Development of the prostate is ... Prostate cancer screening is controversial. Prostate-specific antigen (PSA) testing increases cancer detection but does ...
doi:10.1038/ki.2015.178. PMID 26108068. Mreich E, Chen XM, Zaky A, Pollock CA, Saad S (June 2015). "The role of Krüppel-like ... It has two nuclear localization sequences that signals it to localize to the nucleus. In embryonic stem cells (ESCs), KLF4 has ... Mao Z, Song S, Zhu Y, Yi X, Zhang H, Shang Y, Tong T (July 2003). "Transcriptional regulation of A33 antigen expression by gut- ... Shields JM, Yang VW (July 1997). "Two potent nuclear localization signals in the gut-enriched Krüppel-like factor define a ...
doi:10.1038/ki.2012.414. PMID 23302715. Castillo JJ (2016). "Plasma Cell Disorders". Primary Care. 43 (4): 677-691. doi:10.1016 ... a monoclonal antibody against B-cell maturation antigen (BCMA), also known as CD269, indicated for the treatment of adults with ... an orally available selective inhibitor of nuclear export indicated in combination with dexamethasone in people who have ... 67. Switzerland: S. Karger AG. p. 105. ISBN 978-3-8055-6460-1. Archived from the original on 2016-05-27. Kyle RA, Rajkumar SV ( ...
Prostate specific membrane antigen is a transmembrane carboxypeptidase and exhibits folate hydrolase activity. This protein ... The expression of Ki-67 by immunohistochemistry may be a significant predictor of patient outcome for men with prostate cancer. ... AR, an androgen-activated transcription factor, belongs to the steroid nuclear receptor family. Development of the prostate is ... Options include the digital rectal exam (DRE) and the prostate-specific antigen (PSA) blood test. Such screening is ...
Primary biliary cholangitis
March 2006). "Increased expression of Nuclear Envelope gp210 Antigen in Small Bile Ducts in Primary Biliary Cirrhosis". Journal ... Mells GF, Floyd JA, Morley KI, et al. (April 2011). "Genome-Wide Association study Identifies 12 New Susceptibility Loci for ... Nesher G, Margalit R, Ashkenazi YJ (April 2001). "Anti-Nuclear Envelope Antibodies: Clinical Associations". Seminars in ... including the gp210 and p62 nuclear pore proteins. Gp210 has increased expression in the bile duct of anti-gp210 positive ...
Prostaglandin EP4 receptor
... antigen-presenting cells located primarily in the skin and mucus membranes) to mature, migrate, and direct the early stage of ... thereby suppressing a cells ability to activate nuclear factor kappa B, a transcription factor that controls genes coding for ... Ki=3 nM) (http://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=343). Several synthetic compounds, e.g. 1- ... suppressor T cells that modulate the immune system to maintain tolerance to self-antigens and prevent autoimmune disease); b) ...
Hahn JY, Cho HJ, Bae JW, Yuk HS, Kim KI, Park KW, Koo BK, Chae IH, Shin CS, Oh BH, Choi YS, Park YB, Kim HS (October 2006). " ... Increased nuclear β-catenin levels have also been noted in basal cell carcinoma (BCC), head and neck squamous cell carcinoma ( ... Ren J, Li Y, Kufe D (May 2002). "Protein kinase C delta regulates function of the DF3/MUC1 carcinoma antigen in beta-catenin ... Kobayashi M, Honma T, Matsuda Y, Suzuki Y, Narisawa R, Ajioka Y, Asakura H (May 2000). "Nuclear translocation of beta-catenin ...
Beta-catenina, a enciclopedia libre
Hahn JY, Cho HJ, Bae JW, Yuk HS, Kim KI, Park KW, Koo BK, Chae IH, Shin CS, Oh BH, Choi YS, Park YB, Kim HS (Oct 2006). "Beta- ... O incremento dos niveis de β-catenina nuclear tamén se detectou no carcinoma de células basais, carcinoma de células ... Ren J, Li Y, Kufe D (May 2002). "Protein kinase C delta regulates function of the DF3/MUC1 carcinoma antigen in beta-catenin ... En conxunto, os resultados sinalan que o aumento da localización nuclear da beta-catenina pode ser importante no progreso da ...
Impact of the COVID-19 pandemic on politics
The Campaign for Nuclear Disarmament's general secretary Kate Hudson criticised the exercise, saying "it jeopardises the lives ... "Megszavazta az Országgyűlés a koronavírus-törvényt, Áder pedig ki is hirdette". 30 March 2020. "Áder János már alá is írta a ... were tested positive for COVID-19 from a rapid antigen test after being exposed to people who were not wearing a mask while ... 67-85, doi:10.3998/mpub.11927713.6, ISBN 978-0-472-90246-0, JSTOR 10.3998/mpub.11927713.6 "China is winning the coronavirus ...
... and a molecule called proliferating cell nuclear antigen, or PCNA, which speeds DNA replication and repair by helping to attach ... Wang H, Bauzon F, Ji P, Xu X, Sun D, Locker J, Sellers RS, Nakayama K, Nakayama KI, Cobrinik D, Zhu L (January 2010). "Skp2 is ... Welch PJ, Wang JY (November 1993). "A C-terminal protein-binding domain in the retinoblastoma protein regulates nuclear c-Abl ... Craven RJ, Cance WG, Liu ET (September 1995). "The nuclear tyrosine kinase Rak associates with the retinoblastoma protein pRb ...
The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated...
The antigen defined by mAb Ki-67 is a human nuclear protein the expression of which is strictly associated with cell ... The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated ... The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated ... Ki-67 detects a double band with apparent molecular weights of 395 and 345 kD in immunoblots of proteins from proliferating ...
Augmentation of type IV collagenase, laminin receptor, and Ki67 proliferation antigen associated with human colon, gastric, and...
... and Ki67 proliferation-associated antigen increased during the progression of human colon, gastric, and breast carcinomas. ... Nuclear Proteins / metabolism* * Receptors, Immunologic / metabolism* * Receptors, Laminin * Stomach Neoplasms / metabolism* * ... Augmentation of type IV collagenase, laminin receptor, and Ki67 proliferation antigen associated with human colon, gastric, and ... and Ki67 proliferation-associated antigen increased during the progression of human colon, gastric, and breast carcinomas. ...
Ki-67 | ALZFORUM
Detection of the Ki-67 antigen in fixed and wax-embedded sections with the monoclonal antibody MIB1
One such antibody, MIB1, recognizes a fixation and embedding resistant epitope on the Ki-67 protein if sections are previously ... Novel antibodies have been generated by immunizing with bacterially expressed fragments of the repetitive motif of the Ki-67 ... Other markers of proliferation (e.g. proliferating cell nuclear antigen) have been shown to be expressed in DNA repair, thus we ... Detection of the Ki-67 antigen in fixed and wax-embedded sections with the monoclonal antibody MIB1 Histopathology. 1993 Apr;22 ...
Gamma-Tocotrienol Stimulates the Proliferation, Differentiation, and Mineralization in Osteoblastic MC3T3-E1 Cells
S. E. Johnson and R. E. Allen, "Proliferating cell nuclear antigen (PCNA) is expressed in activated rat skeletal muscle ... mRNA levels of Ki67 and PCNA were determined by real-time PCR. (d) Protein levels of PCNA were determined by Western blot. Data ... The nuclear morphological alteration of MC3T3-E1 cells was shown in Figure 1(d); there were no nuclear morphological alteration ... The results were shown in Figures 2(b) and 2(c); mRNA levels of Ki-67 and PCNA were obviously upregulated by treatment with 2-8 ...
Ki-67 (7B11) | ALZFORUM
September 1995 - Volume 16 - Issue 5 : Otology & Neurotology
Low Power Laser Irradiation Stimulates Cell Proliferation via Proliferating Cell Nuclear Antigen and Ki-67 Expression during...
Low Power Laser Irradiation Stimulates Cell Proliferation via Proliferating Cell Nuclear Antigen and Ki-67 Expression during ... Low Power Laser Irradiation Stimulates Cell Proliferation via Proliferating Cell Nuclear Antigen and Ki-67 Expression during ... potential of these laser doses under study were evaluated by monitoring proliferating cell nuclear antigen and Ki-67 following ... The percentages of Ki-67 or PCNA positive cells were determined by counting positive nuclei (Ki-67/PCNA) and total nuclei in ...
Expression of Ki67 Nuclear Antigen in IELs. Nonneoplastic mammary tissue had immunoreactivity for Ki67 in a few cells (Fig. 3A2 ... Nuclear staining was scored for expression of ER, PR, and Ki67. ER, PR, and Ki67 expression in IELs and tumor tissues was ... neu receptors and Ki67 nuclear antigen (DakoCytomation). Some IELs could not be evaluated immunohistochemically because ... Expression of ER, Ki67, and HER-2 proteins in IELs in canine mammary tissues. A1 to E1, ER. A2 to E2, Ki67. A3 to E3, HER-2. A1 ...
K67LI - Ki-67 antigen-labeling index | AcronymAttic
K67LI is defined as Ki-67 antigen-labeling index rarely. ... How is Ki-67 antigen-labeling index abbreviated? K67LI stands ... Serum SCC antigen may be a useful biologic marker in patients with sinonasal IP [205-207]. Proliferating cell nuclear antigen ... n.d.) Acronym Attic. (2019). Retrieved August 24 2019 from https://www.acronymattic.com/Ki_67-antigen_labeling-index-(K67LI). ... S.v. "K67LI." Retrieved August 24 2019 from https://www.acronymattic.com/Ki_67-antigen_labeling-index-(K67LI).html ...
Ki-67 (protein) - Wikipedia
Gerdes J, Schwab U, Lemke H, Stein H (1983). "Production of a mouse monoclonal antibody reactive with a human nuclear antigen ... Antigen KI-67 also known as Ki-67 or MKI67 (Marker Of Proliferation Ki-67) is a protein that in humans is encoded by the MKI67 ... Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis. The Ki-67 protein (also known as MKI67) is a ... "Entrez Gene: Antigen identified by monoclonal antibody Ki-67". Schonk DM, Kuijpers HJ, van Drunen E, van Dalen CH, Geurts van ...
The tetraspanin CD9 controls migration and proliferation of parietal epithelial cells and glomerular disease progression |...
To assess cell proliferation, we used the nuclear antigen Ki67 staining. After overnight starvation in ECBM + 1% FBS, cells ... PV represents total podocyte volume and PA the combination of nuclear (p57+) and cytoplasmic (SNP+) areas per glomerular cross- ... The CD9 antigen is strongly expressed on platelets26 and platelets are activated in CGN, raising the hypothesis of a role for ... Molecular cloning of the CD9 antigen. A new family of cell surface proteins. J. Biol. Chem. 266, 117-122 (1991). ...
The diagnostic and prognostic value of genetic aberrations in resectable distal bile duct cancer
Anti-Ki-67 antibodies, human and mouse - Primary antibodies - Antibodies - MACS Flow Cytometry - Products - Miltenyi Biotec - 日本
... a nuclear and nucleolar protein, which is strictly associated with cell proliferation. Two Ki-67 isoforms with molecular weight ... Molecular mass of antigen [kDa]: 359 * Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium ... a nuclear and nucleolar protein, which is strictly associated with cell proliferation. Two Ki-67 isoforms with molecular weight ... Takagi, M. et al. (2001) A novel nucleolar protein, NIFK, interacts with the forkhead associated domain of Ki-67 antigen in ...
Anti-Ki-67 antibodies, human and mouse - Primary antibodies - Antibodies - MACS Flow Cytometry - Products - Miltenyi Biotec -...
... a nuclear and nucleolar protein, which is strictly associated with cell proliferation. Two Ki-67 isoforms with molecular weight ... Molecular mass of antigen [kDa]: 359 * Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The ... a nuclear and nucleolar protein, which is strictly associated with cell proliferation. Two Ki-67 isoforms with molecular weight ... Takagi, M. et al. (2001) A novel nucleolar protein, NIFK, interacts with the forkhead associated domain of Ki-67 antigen in ...
Frontiers | Altered methylation and expression of ER-associated degradation factors in long-term alcohol and constitutive ER...
... liver immunohistochemistry with anti-proliferative cell nuclear antigen Ki-67 antibodies. Original magnification: ×200. (D) ... nuclear protein 1), Chop, Trib3 (tribbles homolog 3), Gadd45, and FoxO, some nuclear factor-kappaB (NFκB) targeted genes ... The number of Ki-67 positive hepatocytes was significantly increased in the KO compared to the WT (Figures 2C,D). More ... Proteins (whole or nuclear) were extracted respectively from WT liver tissues, KO without liver tumors and the normal liver ...
Induction of solid tumor differentiation by the peroxisome proliferator-activated receptor-γ ligand troglitazone in patients...
The percentage of cells with strong nuclear staining for Ki-67 was quantified by immunohistochemical staining with the MIB-1 ... Standard quantification was performed by assessing the percentage of cells with positive nuclear staining for this antigen. ... Expression of the Ki-67 nuclear antigen, a reliable marker of cellular proliferation (22), was assayed by immunohistochemical ... Nuclear expression of PPARγ protein in liposarcoma tissue. Immunohistochemical staining of a biopsy sample of liposarcoma ...
Ma CK[au] - PubMed - NCBI
Prognostic value of proliferating cell nuclear antigen index in gastric stromal tumors. Correlation with mitotic count and ... Ki-67 and proliferating cell nuclear antigen tumor proliferative indices in DNA diploid colorectal adenocarcinomas. Correlation ... study of 20 cases with immunohistochemical assessment of cell differentiation and the prognostic role of proliferation antigens ...
Asxl1 exerts an antiproliferative effect on mouse lung maturation via epigenetic repression of the E2f1-Nmyc axis | Cell Death ...
a Cell proliferation was determined by immunostaining with Ki-67 and PCNA at E18.5. Scale bar, 100 μm. b mRNA expression levels ... Sections were processed for antigen retrieval in 0.01 M citric acid solution (pH 6.0) for 15 min. After blocking with blocking ... We previously demonstrated that ASXL1 is primarily located in the nucleus and regulates nuclear hormone receptors in ... Cell proliferation was measured by staining for the two proliferation markers Ki-67 and proliferating cell nuclear antigen ( ...
Embryonic stem cells develop into functional dopaminergic neurons after transplantation in a Parkinson rat model | PNAS
... mouse anti-proliferating cell nuclear antigen and goat anti-Ki 67 (both from Santa Cruz Biotechnology; 1:100), rat anti-M6 ( ... was consistent with absence of staining against proliferating cellular nuclear antigen or the proliferation marker Ki 67 in the ... Hybridoma Bank, Iowa City, Iowa; 1:1,000), and mouse anti-stem cell-specific embryonic antigen 1 (Hybridoma Bank, 2.5 μg/ml) ... both from the host and the grafted ES cells as indicated by the absence or presence of colabeling with mouse-specific nuclear ...
Ovarian follicle activation - Wikipedia
Dilated Cardiomyopathy Pathology: Overview, Etiology, Epidemiology
17] along with regeneration of myocytes with the expression of proliferating cell nuclear antigen (PCNA) and Ki-67.  ... Note the variation in nuclear size. The change is nonspecific and can be seen in heart failure from any cause. View Media ... The most commonly identified are mutations of the LMNA gene, encoding the lamin-A and -C nuclear envelope proteins; these ... Note the variation in nuclear size. The change is nonspecific and can be seen in heart failure from any cause. ...
Penile Cancer: Background, History of the Procedure, Problem
PerCP/Cyanine5.5 anti-human Ki-67 Antibody anti-Ki-67 - Ki-67
Antigen Ki-67 is a nuclear protein expressed as two isoforms with molecular weights of 395 and 345 kD. ... Nuclear Markers Antigen References 1. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.. 2. Yerushalmi R, et al. 2010. ... Antigen Ki-67 Isotype Mouse IgG1, κ Ave. Rating Submit a Review Product Citations publications PHA-activated human peripheral ... Antigen Ki-67 is a nuclear protein expressed as two isoforms with molecular weights of 395 and 345 kD. Both isoforms contain ...
Identification of a novel plant MAR DNA binding protein localized on chromosomal surfaces, Plant Molecular Biology | 10.1007...
Changes in distribution of nuclear matrix antigens during the mitotic cell cycle ... Ki-67 detects a nuclear matrix-associated proliferationrelated antigen. II. Localization in mitotic cells and association with ... Purification and immunological detection of pea nuclear intermediate laments: evidence for plant nuclear lamins ... Nucleic-acid-binding properties of hnRNP-U/SAF-A, a nuclear-matrix protein which binds DNA and RNA in vivo and in vitro ...
Activation of Postnatal Neural Stem Cells Requires Nuclear Receptor TLX | Journal of Neuroscience
Ki67, PCNA (proliferating cell nuclear antigen), and MCM2 are endogenous markers that were used to label the status of Nes-GFP+ ... 1984) Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J ... 4A). However, 7 d later the number of Ki67+GFP+ cells dropped ,8-fold to 2.4% in the mutant brains, while Ki67+GFP+ cells from ... 1991) Proliferating cell nuclear antigen (PCNA)/cyclin expression during the cell cycle in normal and leukemic cells. Leuk Res ...
KAKEN - Research Projects | 放射線感受性予測の基礎研究 (KAKENHI-PROJECT-09255102)
Publications] Okuno, Y. et al.: Prognostic values of proliferating cell nuclear antigen (PCNA) and Ki-67 for radiotherapy of ... Analysis of results with Ki 67 index.Int. J. Radiat. Oncol. Biol. Phys.. 45(in press). (2000). *. Related Report. 1999 Annual ... Publications] Ogawa,Y.et al.: Expression of fas(CD95/APO-1)antigen induced by radiation therapy for diffuse B-cell lymphoma: ... Publications] Nishioka, A. et al.: An augmentation of Fas(CD95/APO-1) antigen induced by radiation : Flow cytometry analysis ...
CiNii 論文 - Prognostic and Predictive Factors in the Adjuvant Treatment of Breast Cancer ...
Cell cycle analysis of cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67 GERDES J. ... Ki-67 immunostaining node-negative stage I/ II breast carcinoma. Significant correlation with prognosis SAHIN AA. ... Long-term prognostic value of growth fraction determination by Ki-67 immunostaining in primary operable breast cancer PIERGA J ... The efficacy and limitations of repeated slide conferences for improving interobserver agreement when judging nuclear atypia of ...
KEGG BRITE: KEGG Orthology (KO) - Falco cherrug (Saker falcon)
... antigen KI-67 K14559 MPP10; U3 small nucleolar RNA-associated protein MPP10 K10356 MYO1; myosin I K10356 MYO1; myosin I K10356 ... 102048903 CSRNP1; cysteine/serine-rich nuclear protein 1 102045809 CSRNP3; cysteine/serine-rich nuclear protein 3 isoform X2 ... cysteine/serine-rich nuclear protein K17494 CSRNP; cysteine/serine-rich nuclear protein K08889 FER; tyrosine-protein kinase Fer ... 102045741 PPP1R8; nuclear inhibitor of protein phosphatase 1 102050984 neurabin-1-like isoform X2 102046573 PPP1R9A; neurabin-1 ...
Sabine Girod | Stanford Medicine Profiles
... activity is possible by immunohistochemical staining with monoclonal antibodies against proliferating nuclear antigen and Ki-67 ... P53 AND KI-67 EXPRESSION IN PRENEOPLASTIC AND NEOPLASTIC LESIONS OF THE ORAL-MUCOSA INTERNATIONAL JOURNAL OF ORAL AND ... Ki 67 was used as a proliferation marker. The results imply that expression of mutated p53 is an indicator for potential ... A complete network of nuclear receptors has now been identified that mediate the action of retinoids and can interfere directly ...
AntibodyProliferationIsotypePCNAImmunohistochemistryAnti-Ki-67ProteinsHigh Ki-67 expressionAntibodiesImmunohistochemical expressionExpressionAssociation of Ki-67ReactivityTumorsRecombinantRetrievalEpitopeSpecificityFormalin-fixedOverexpressionCloneReceptorsCellMarkersLocalizationPrognostic significanceCDNACellsCorrelationReceptorIsoformsGrowth fractionFractionSynthesisTissueIndexRecurrenceHuman0.01
- The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins. (rupress.org)
- One such antibody, MIB1, recognizes a fixation and embedding resistant epitope on the Ki-67 protein if sections are previously microwaved in a citrate buffer. (nih.gov)
- Thus, MIB1 represents a new anti-Ki-67 antibody which appears to be a robust marker of cell proliferation easily applicable to archival material. (nih.gov)
- Antigen KI-67 also known as Ki-67 or MKI67 (Marker Of Proliferation Ki-67) is a protein that in humans is encoded by the MKI67 gene (antigen identified by monoclonal antibody Ki-67). (wikipedia.org)
- One of its primary advantages over the original Ki-67 antibody (and the reason why it has essentially supplanted the original antibody for clinical use) is that it can be used on formalin-fixed paraffin-embedded sections, after heat-mediated antigen retrieval (see next section below). (wikipedia.org)
- The Ki-67 protein was originally defined by the prototype monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the Hodgkin lymphoma cell line L428. (wikipedia.org)
- Immunofluorescent antibody staining against neurofilament (green) and Ki-67 (red) in a mouse embryo 12.5 days after fertilization. (wikipedia.org)
- Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. (biolegend.com)
- 7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes. (biolegend.com)
- A molecule that is capable of binding to an antibody or to an antigen receptor on a T cell, especially one that induces an immune response. (thefreedictionary.com)
- Baisch, H., Gerdes, J. Simultaneous staining of exponentially growing versus plateau phase cells with the proliferation-associated antibody Ki-67 and propidium iodide: analysis by flow cytometry. (protocol-online.org)
- The Ki-67 antigen is a human nuclear protein defined by its reactivity with monoclonal antibody from the Ki-67 clone. (beckman.com)
- The nanoparticles were coated with 3-aminopropyl triethoxysilane (APTES) and further modified with biomolecules, such as Biotin-Anti-rabbit (mouse IgG) and rabbit antibody-AntiKi-67, through a conjugation method. (spiedigitallibrary.org)
- There are currently no images for Ki-67/MKI67 Antibody (NB500-170F). (novusbio.com)
- This Ki67 antibody is useful for Immunohistochemistry on frozen and paraffin-embedded sections and Immunocytochemistry/Immunofluorescence. (novusbio.com)
- This antibody will not work without optimal antigen retrieval. (novusbio.com)
- Originally discovered employing mouse monoclonal antibody against a nuclear antigen from Hodgkin's lymphoma-derived cell line, this non-histone protein was named Ki67 after researcher's location (Gerdes and colleagues), Ki for Kiel University in Germany and 67 referring to the clone number on the 96-well plate. (novusbio.com)
- Brown DC, Gatter KC (1990) Monoclonal antibody Ki-67: its use in histopathology. (springer.com)
- The mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells. (exbio.cz)
- Schlüter C, Duchrow M, Wohlenberg C, Becker MH, Key G, Flad HD, Gerdes J: The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins. (exbio.cz)
- Duchrow M, Schlüter C, Key G, Kubbutat MH, Wohlenberg C, Flad HD, Gerdes J: Cell proliferation-associated nuclear antigen defined by antibody Ki-67: a new kind of cell cycle-maintaining proteins. (exbio.cz)
- Gerdes J, Lemke H, Baisch H, Wacker HH, Schwab U, Stein H: Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. (exbio.cz)
- Gerdes J, Schwab U, Lemke H, Stein H: Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. (exbio.cz)
- HT-29 xenograft tumor in mouse 20x pretreatment w/ citrate pH 6.0 using anti-Ki67 antibody GTX20833 at 5 ug/mL for 30 min at RT. (genetex.com)
- Immunohistochemical analysis of formalin-fixed paraffin-embedded human tonsil using Ki-67 antibody. (genetex.com)
- The antigen defined by mAb Ki-67 is a human nuclear protein the expression of which is strictly associated with cell proliferation and which is widely used in routine pathology as a "proliferation marker" to measure the growth fraction of cells in human tumors. (rupress.org)
- Ki-67 antigen-specific antisense oligonucleotides inhibit the proliferation of IM-9 cell line cells, indicating that the Ki-67 antigen may be an absolute requirement for maintaining cell proliferation. (rupress.org)
- The proportion of neoplastic cells immunocytochemically positive for type IV collagenase (IVase), laminin receptor (LR), and Ki67 proliferation-associated antigen increased during the progression of human colon, gastric, and breast carcinomas. (nih.gov)
- Other markers of proliferation (e.g. proliferating cell nuclear antigen) have been shown to be expressed in DNA repair, thus we investigated expression of MIB1 immunoreactivity in situations of DNA repair in vivo--ultraviolet irradiated human skin. (nih.gov)
- Clone REA183 recognizes the Ki-67 antigen, a nuclear and nucleolar protein, which is strictly associated with cell proliferation. (miltenyibiotec.com)
- Antigen KI-67 is a nuclear protein that is associated with cellular proliferation. (wikipedia.org)
- Altering Ki-67 expression levels did not significantly affect cell proliferation in vivo. (wikipedia.org)
- The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation, and can be used in immunohistochemistry. (wikipedia.org)
- Ki-67 and MIB-1 monoclonal antibodies are directed against different epitopes of the same proliferation-related antigen. (wikipedia.org)
- There was also a marked reduction in immunohistochemical expression of Ki-67, a marker of cell proliferation. (pnas.org)
- Other nuclear receptors that regulate cellular differentiation and proliferation pathways also may represent promising targets for novel therapeutic strategies in cancer treatment. (pnas.org)
- Chapter 3 Prognostic value of cell proliferation (Ki-67 antigen) and nuclear DNA content in clinically resectable, distal bile duct carcinoma. (uva.nl)
- Ki-67 is required for cell proliferation and its expression is restricted to the phases G 1 , S, G 2 , and M of the cell cycle. (biolegend.com)
- Ki-67 has also been used to study myocyte proliferation after myocardial infarction as well as lymphocyte proliferation during infection, and has been used in neurons of patients with different neuropathologies. (biolegend.com)
- It was shown that two proliferation markers, Ki-67 and proliferating cell nuclear antigen (PCNA), are regulated by these factors. (wikipedia.org)
- Thus, Ki-67 reactivity, defined as percent tumor cells staining positive as measured by immunohistochemical (IHC) staining, is a specific nuclear marker for cell proliferation. (questdiagnostics.com)
- Landberg, G., Roos, G. Flow cytometric analysis of proliferation associated nuclear antigens using washless staining of unfixed cells. (protocol-online.org)
- Tabaczka P. A flow cytometric method for measuring lymphocyte proliferation directly from tissue culture plates using Ki-67 and propidium iodide. (protocol-online.org)
- The cell proliferation antigen Ki-67 organises heterochromatin. (abcam.com)
- Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. (biolegend.com)
- Ki-67 nuclear antigen is associated with cell proliferation and is found throughout the cell cycle (G1, S, G2, M phases). (beckman.com)
- RESEARCH DESIGN AND METHODS- Changes in islet protein levels of cyclins and of two critical cell cycle regulators cyclin kinase inhibitor p27 and S-phase kinase-associated protein 2 (Skp2) were assessed in mice treated with exendin-4 and in a mouse model with specific upregulation of nuclear cAMP signaling exhibiting increased β-cell proliferation (CBP-S436A mouse). (diabetesjournals.org)
- Transgenic β-cell-specific overexpression of phosphorylation-defective, nuclear FoxO1 results in reduced nuclear PDX-1, reduced β-cell number and islet size, and diminished β-cell proliferation response to exendin-4 ( 5 , 6 ). (diabetesjournals.org)
- In addition, we have used a mouse model with specific nuclear upregulation of cAMP-CREB-CREB binding protein (CBP) action and enhanced in vivo and in vitro β-cell proliferation ( 9 ) to specifically assess the role of cAMP-CREB-regulated transcription on cell cycle regulation. (diabetesjournals.org)
- Due to its association with cell divison process, Ki-67 is routinely used as cellular proliferation marker of solid tumors as well as certain hematological malignancies, and a correlation has been demonstrated between Ki-67 index and the histopathological grade of cancers. (novusbio.com)
- AEG-1-mediated regulation of astrocyte proliferation was assayed by quantifying the levels of cell proliferation markers, Ki67 and proliferation cell nuclear antigen, using immunocytochemistry. (biomedcentral.com)
- Ki67 is routinely used as a neuronal marker of cell cycling and proliferation. (biomol.com)
- Cell proliferation was quantitated by direct cell counting and by immunocytochemistry for the nuclear Ki-67 antigen. (arvojournals.org)
- In this study, the clinical utility of proliferation indices (labeling index, Li) based on immunohistochemistry targeted at antigens Ki-67 and High-mobility group A1 (HMGA-1) for prediction of NFPA prognosis was investigated. (springer.com)
- Gerdes J: Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies. (exbio.cz)
- Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes were fixed and permeabilized with 70% ethanol, and then stained with Ki-67 (clone 16A8) Brilliant Violet 605™ (filled histogram) or rat IgG2a, κ Brilliant Violet 605™ isotype control (open histogram). (biolegend.com)
- The percentages of Ki-67 or PCNA positive cells were determined by counting positive nuclei (Ki-67/PCNA) and total nuclei in five random fields per tissue sections. (manipal.edu)
- PCNA - Proliferating Cell Nuclear Antigen, expressed during the DNA synthesis. (wikipedia.org)
- A decrease of Ki67 and proliferating cell nuclear antigen (PCNA) as well as an increase of cleaved caspase-3 were observed in HA-treated tumors, along with increasing apoptotic cells, obtained by TUNEL assay. (mdpi.com)
- The results obtained indicate that PCNA may be helpful to evaluate the tumor grade, better than Ki-67, for feline fibrosarcomas. (biomedcentral.com)
- These similarities with incomplete intestinal metaplasia are present on histology, mucin histochemistry, and immunohistochemistry with various differentiation markers (cytokeratins and MUC antigens). (bmj.com)
- Ki-67 detects a double band with apparent molecular weights of 395 and 345 kD in immunoblots of proteins from proliferating cells. (rupress.org)
- We conclude that the Ki-67 antigen defines a new category of cell cycle-associated nuclear nonhistone proteins. (rupress.org)
- The N-terminal portion of Ki-67 contains a forkhead associated (FHA)1 domain, which is involved in interaction with proteins such as Hklp2 and NIFK. (miltenyibiotec.com)
- Nuclear translocation and regulation of intranuclear distribution of cytoplasmic poly(A)-binding protein are distinct processes mediated by two Epstein Barr virus proteins. (abcam.com)
- The expression of p53, MDM2, and p21 proteins and the value of the Ki-67 index were analyzed for 244 patients. (aacrjournals.org)
High Ki-67 expression4
- High Ki-67 expression in an invasive breast cancer, with cancer nuclei being stained (brown). (wikipedia.org)
- Prognostic and clinicopathological role of high Ki-67 expression in patients with renal cell carcinoma: a systematic review and meta-analysis. (questdiagnostics.com)
- Sensitivity analysis and cumulative meta-analysis further demonstrated that the association between high Ki-67 expression and poor overall survival of ovarian cancer patients was stable and reliable. (springermedizin.de)
- High Ki-67 expression is significantly related to poor overall survival and may serve as a prognostic biomarker for ovarian cancer patients. (springermedizin.de)
- Novel antibodies have been generated by immunizing with bacterially expressed fragments of the repetitive motif of the Ki-67 gene. (nih.gov)
- An avidin-biotin peroxidase method was performed using monoclonal antibodies against p53, MDM2, p21, and Ki-67 antigens after antigen retrieval treatment of formalin-fixed specimens. (aacrjournals.org)
- Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms. (biomol.com)
- Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67-positive cells among the total number of resting cells = Ki67 index). (genetex.com)
- Cattoretti G, Becker MH, Key G, Duchrow M, Schluter C, Galle J et al (1992) Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MIB 1 and MIB 3) detect proliferating cells in microwave-processed formalin-fixed paraffin sections. (springermedizin.de)
- The clinicopathological features and immunohistochemical expression of FAS and Ki-67 antigen were studied. (aacrjournals.org)
- Finally, the morphological and cell cycle distribution of MIB1 expression is identical to that of Ki-67. (nih.gov)
- Ki67 expression was significantly higher in high-grade DCIS than in hyperplasia or low-grade DCIS. (aacrjournals.org)
- Clinicopathological and prognostic value of Ki-67 expression in bladder cancer: a systematic review and meta-analysis. (questdiagnostics.com)
- Ki-67 expression in pulmonary tumors. (questdiagnostics.com)
- Takahashi M. The cell cycle associated change of the Ki-67 reactive nuclear antigen expression. (protocol-online.org)
- Nuclear bodies reorganize during myogenesis in vitro and are differentially disrupted by expression of FSHD-associated DUX4. (abcam.com)
- Expanded GAA repeats impair FXN gene expression and reposition the FXN locus to the nuclear lamina in single cells. (abcam.com)
- The aim of the study was to determine whether the expression of the cell cycle markers p53, MDM2, p21, and Ki-67 was predictive of superficial bladder cancer recurrence and to compare the relative predictive power for tumor recurrence of a cell cycle index based on the number of abnormally expressed cell cycle markers with a clinicopathological index based on primary clinical tumor characteristics. (aacrjournals.org)
- The χ 2 test was performed to describe the correlation between the Ki-67 index and p53, MDM2, and p21 protein expression. (aacrjournals.org)
- Expression of Ki-67 occurs during G1, S, G2, and M phase, while in G0 phase the Ki-67 protein is not detectable. (biolegend.com)
- The level of Ki-67 antigen expression varies during cell cycle and it has been correlated to several cell pathways: The Ki-67 decrease pathway is characterized by a declining Ki-67 staining and leads eventually to the exit from the active cell cycle (G0). (beckman.com)
- Its expression occurs specially during late G1, S, G2 and M phases of the cell cycle, while in cells undergoing G0 phase, Ki67 remains undetectable. (novusbio.com)
- Ki67 undergoes phosphorylation/dephosphorylation during mitosis, is susceptible to proteases and its structure implies that its expression is regulated by proteolytic pathways. (novusbio.com)
- Aberrant expression of minichromosome maintenance protein-2 and Ki67 in laryngeal squamous epithelial lesions. (nih.gov)
- Importantly, there was minimal expression of Mcm-2 or Ki67 in the most superficial layers of normal larynx and abnormal or atypical hyperplasia, in contrast to carcinoma in situ and SCC. (nih.gov)
- The prognostic significance of Ki-67 expression in patients with ovarian cancer was controversial in various studies. (springermedizin.de)
- The pooled hazard ratios and 95% confidence intervals were calculated to assess the prognostic significance of Ki-67 expression for overall survival in ovarian cancer patients. (springermedizin.de)
- Proliferative Activity of Canine Mast Cell Tumours Evaluated by Bromodeoxyuridine Incorporation and Ki-67 Expression," Journal of Comparative Pathology, Vol. 127, No. 4, 2002, pp. 233-238. (scirp.org)
- The aim of this study was to examine the expression rate of EGFR and Ki-67 to clarify their role in the biological behavior of the lesions. (rroij.com)
- Ki-67 expression was close to that of EGFR. (rroij.com)
Association of Ki-671
- Due to strict association of Ki-67 with proliferating cells, Ki-67 is often used as an indicator of the "growth fraction" of a given cell population. (miltenyibiotec.com)
- In addition, Ki-67 reactivity is included among other parameters in the World Health Organization's recommended grading system for neuroendocrine tumors of the pancreas and gastrointestinal tract. (questdiagnostics.com)
- Higher Ki-67 reactivity in tumor tissue is associated with adverse outcomes. (questdiagnostics.com)
- Gastric carcinomas classified as well-differentiated advanced stage contained a significantly higher proportion of tumor cells positive for IVase (P less than 0.001), LR (P less than 0.001), and Ki67 (P less than 0.001) compared with well-differentiated superficial tumors. (nih.gov)
- Gastric carcinomas classified as poorly differentiated superficial had a significantly higher proportion of cells positive for Ki67 (P less than 0.016), but not IVase (P less than 0.069) or LR (P less than 0.075), compared with poorly differentiated advanced tumors. (nih.gov)
- Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors. (biolegend.com)
- The correlation between low Ki67 index and histologically low-grade tumors is strong. (biomol.com)
- Growing residual tumors showed a trend towards higher Ki-67 Li compared with stable ones ( p = 0.104). (springer.com)
- In residual adenomas with a Ki-67 Li above 2.2 %, regrowth should be expected, and these tumors may require shorter intervals of follow-up magnetic resonance imaging (MRI) and/or early adjuvant therapy. (springer.com)
- J. J. Abadie, M. A. Amardeilh and M. E. Delverdier, "Immunohistochemical Detection of Proliferating Cell Nuclear Antigen and Ki-67 in Mast Cell Tumors from Dogs," Journal of the American Animal Hospital Association, Vol. 215, No. 11, 1999, pp. 1629-1634. (scirp.org)
- E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa. (biolegend.com)
- Here we examined the effects of various antigen retrieval methods, including microwave irradiation and autoclave treatment, for immunohistochemical detection of AR in cultured LNCaP cells and paraffin embedded sections of human prostate, and found beneficial effects of autoclave treatment over microwave treatment. (nii.ac.jp)
- The central part of the Ki-67 antigen cDNA contains a large 6,845-bp exon with 16 tandemly repeated 366-bp elements, the "Ki-67 repeats", each including a highly conserved new motif of 66 bp, the "Ki-67 motif", which encodes for the epitope detected by Ki-67. (rupress.org)
- Both isoforms contain one forkhead-associated domain and 16 concatenated "Ki-67 repeats," each containing the epitope recognized by the mAb Ki-67. (biolegend.com)
- The recommended ELISA Kit will likely detect the antigen in question with higher specificity in approved samples than the available alternatives. (antibodies-online.com)
- Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. (novusbio.com)
- 11 Ki-67 overexpression is associated with worse survival of individuals with a neuroendocrine tumor. (questdiagnostics.com)
- Surface staining of CD25 (clone MEM-181 APC) was followed by permeabilization and nuclear staining of Ki-67 (clone Ki-67 PE). (exbio.cz)
- Finally, 0.01M EDTA (pH7.4) gave us the most intense nuclear signal for the steroid hormone receptors, though the best soaking solution was 0.01M citrate buffer (pH6.0) considering the lost of tissue morphology. (nii.ac.jp)
- Subsequently, tissue regeneration potential of these laser doses under study were evaluated by monitoring proliferating cell nuclear antigen and Ki-67 following the laser treatment and comparing it with the un-illuminated controls. (manipal.edu)
- Proliferating cell nuclear antigen labeling index . (acronymattic.com)
- During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. (wikipedia.org)
- Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent in resting (quiescent) cells (G0). (wikipedia.org)
- Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. (wikipedia.org)
- In breast cancer Ki67 identifies a high proliferative subset of patients with ER-positive breast cancer who derive greater benefit from adjuvant chemotherapy Ki-67 is an excellent marker to determine the growth fraction of a given cell population. (wikipedia.org)
- There is tumor cell positivity in 70% of the cells: Ki-67 labelling index = 70% Counting positive versus negative nuclei with Ki-67 labeling, in this case in a neuroendocrine tumor of the small intestine. (wikipedia.org)
- Dividing cells show strong Ki-67 staining in cell nuclei while all cells contain large amounts of tubulin, the major component of microtubules. (wikipedia.org)
- Ki-67 immunostaining and other prognostic factors including tobacco smoking in patients with resected nonsmall cell lung carcinoma. (questdiagnostics.com)
- LCs and dermal DCs are both well equipped to capture environmental antigens, migrate to the draining lymph nodes, and initiate specific T cell immune responses playing a critical role in skin immunity ( 1 , 10 , 11 ). (rupress.org)
- The Ki-67 antigen is expressed at active phases of the cell cycle (G1, S, G2 and M phases), but it is absent in resting cells (G0 phase). (beckman.com)
- The cellular localization of the Ki-67 protein is cell cycle phase dependent. (beckman.com)
- The antigen is rapidly degraded as the cell enters the non proliferative state. (beckman.com)
- Strikingly, the majority of the P/P divisions that formed the retinal four-cell clones were found to be asymmetric, with paired mitotic daughters differing in the trajectory of interkinetic nuclear movement, cell cycle length, and the composition of their daughter cells (i.e. granddaughter cells). (biologists.org)
- Ki67 is a large protein with expected molecular weight of about 395 kD and has a very complex localization pattern within the nucleus, one which changes during cell cycle progression. (novusbio.com)
- Ki67 is associated with nucleolar DFC (dense fibrillary component) and its regulation appears to be tightly controlled (estimated half life is 60-90 min, regardless of the cell position in the cell cycle), presumably by precise synthesis and degradation systems involving proteasome, a protease complex. (novusbio.com)
- Twenty patients were evaluated with immunophenotyping, intracellular Ki67 staining, T-cell receptor excision circle (TREC) quantitation in sorted CD4 and CD8 cells, and thymic computed tomography scans prior to and ∼6 and ∼18 months after initiation of HAART. (asm.org)
- Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating. (biomol.com)
- Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). (biomol.com)
- The Ki-67 is a nucle-ar anti-gen -expressed in -the G1, S, G2, -and M phas-es of -the -cell -cycle rec-og-niz-able by -the mono-clo-nal anti-body -MIB-1. (minervamedica.it)
- Sections of normal larynx (n=10), laryngeal dysplasia (n=20) and laryngeal squamous cell carcinoma (SCC) (n=10) were classified according to the Ljubljana classification and stained for markers of cell cycle entry, minichromosome maintenance protein-2 (Mcm-2) and Ki67. (nih.gov)
- Study of immunohistochemical markers (CK-19, CD-56, Ki-67, p53) in differentiating benign and malignant solitary thyroid nodules with special reference to papillary thyroid carcinomas. (questdiagnostics.com)
- The AT-hook motif was essential for matrix attachment region (MAR) binding, and the hydrophobic region of the PPC was indispensable for nuclear localization. (deepdyve.com)
- New mAbs against a bacterially expressed part and a synthetic polypeptide deduced from the isolated cDNA react with the native Ki-67 antigen, thus providing a circle of evidence that we have cloned the authentic Ki-67 antigen cDNA. (rupress.org)
- Computer analysis of the nucleic acid and the deduced amino acid sequence of the Ki-67 antigen confirmed that the cDNA encodes for a nuclear and short-lived protein without any significant homology to known sequences. (rupress.org)
- The percentage of positive cells increased significantly (P less than 0.001) for all three antigens comparing carcinomas with adenomas and Dukes' C stage compared with Dukes' A/B stage. (nih.gov)
- In a wide range of normal tissues the distribution and number of MIB1 immunoreactive cells matched that of cryostat sections stained with Ki-67. (nih.gov)
- Ki-67 mutant mice developed normally and cells lacking Ki-67 proliferated efficiently. (wikipedia.org)
- The fraction of Ki-67-positive tumor cells (the Ki-67 labeling index) is often correlated with the clinical course of cancer. (wikipedia.org)
- Protein Ki-67 in human MCF-7 cells. (wikipedia.org)
- Ki-67 protein (red), tubulin (green) and DNA (blue) in HeLa cells. (wikipedia.org)
- Agonist ligands for the nuclear receptor peroxisome proliferator-activated receptor-γ have been shown to induce terminal differentiation of normal preadipocytes and human liposarcoma cells in vitro . (pnas.org)
- This characteristic makes Ki-67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. (biolegend.com)
- Ki-67 is a nuclear non-histone protein that is present at low levels in quiescent cells but is increased in proliferating cells, especially in the G2, M, and latter half of the S phase. (questdiagnostics.com)
- Immune cells are distributed on both sides of the basement membrane and participate in the defense against pathogens through professional antigen-presenting cells. (rupress.org)
- If cells on this pathway get stimulated by growth factors, they can enter the Ki-67 increase pathway that brings the cells back into S phase. (beckman.com)
- Cells following the Ki-67 stable pathway exhibit a constant intensity of Ki-67 staining during the G1 phase. (beckman.com)
- The Ki-67 nuclear antigen was detected in 10%-15% of cells located in the basal zone of the nodule. (dovepress.com)
- In this study, we investigated the association between breast cancer risk and the frequency of mammary epithelial cells expressing p27, estrogen receptor (ER), and Ki67 in normal breast tissue. (aacrjournals.org)
- We found that the frequency of Ki67 + cells was positively associated with breast cancer risk among premenopausal women [OR = 10.1, 95% confidence interval (CI) = 2.12-48.0]. (aacrjournals.org)
- We counted Ki67-positive and Ki67-negative (outlined) cells in the VZ+SVZ at E13, E14 and E15 to obtain percentage Ki67-positive/Nissl-positive (see Table 1 ). (biologists.org)
- Because these cells are likely to be critical for the genesis and perpetuation of leukemic disease, the present studies sought to characterize unique molecular properties of the LSC population, with particular emphasis on the transcription factor, nuclear factor-κB (NF-κB). (bloodjournal.org)
- Higher magnification (inset) demonstrated a pleomorphic population of medium- to large-sized discohesive round cells containing variable amounts of slightly eosinophilic cytoplasm, irregular nuclear contours, and prominent nucleoli. (mdedge.com)
- Ki-67 is a molecule that can be easily detected in proliferating cells in order to gain an understanding of the rate at which the cells within a tumor are growing. (rroij.com)
- The same pattern of antigen correlation with progression was found with 40 human gastric carcinomas. (nih.gov)
- A positive correlation (P less than 0.001) was found for the immunoreactivity of all three antigens in the invasive carcinomas compared with the normal epithelium. (nih.gov)
- Ekramullah SM, Saitoh Y, Arita N, Ohnishi T, Hayakawa T (1996) The correlation of Ki-67 staining indices with tumour doubling times in regrowing non-functioning pituitary adenomas. (springer.com)
- A critical regulator of terminal differentiation for the adipocytic lineage is a nuclear receptor known as peroxisome proliferator-activated receptor-γ (PPARγ) ( 3 - 9 ). (pnas.org)
- Here, we show that nuclear receptor TLX (NR2E1) controls the activation status of postnatal NSCs in mice. (jneurosci.org)
- We showed previously that the orphan nuclear receptor Tlx is required for the correct establishment of the pallio-subpallial boundary. (jneurosci.org)
- In nude mouse xenografts in which the growth fraction had been defined using a fraction of labelled mitosis method, the labelling index with MIB1 matched that previously determined for Ki-67 and correlated well with the growth fraction. (nih.gov)
- Huber H. The biological and clinical significance of the KI-67 growth fraction in multiple myeloma. (protocol-online.org)
- Biochemically, AHL1 was also found in the nuclear matrix fraction. (deepdyve.com)
- Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis. (wikipedia.org)
- Immunofluorescence for p27, ER, and Ki67 was performed on tissue microarrays constructed from benign biopsies containing normal mammary epithelium and scored by computational image analysis. (aacrjournals.org)
- MIB-1 is used in clinical applications to determine the Ki-67 labelling index. (wikipedia.org)
- The clinical value of Ki-67/MIB-1 labeling index in human astrocytomas. (questdiagnostics.com)
- Prognostic and predictive value of centrally reviewed Ki-67 labeling index in postmenopausal women with endocrine-responsive breast cancer: results from Breast International Group Trial 1-98 comparing adjuvant tamoxifen with letrozole. (questdiagnostics.com)
- The Ki-67 index a prognostic marker in medullary thyroid carcinoma. (questdiagnostics.com)
- The clinical findings, lack of infiltrative border, low Ki-67 index, and low proliferative ability support a diagnosis of sebaceous adenoma. (dovepress.com)
- The Ki-67 labeling index (LI) is con-sid-ered as a mark-er of pro-life-ra-tion (-growth frac-tion -rate), -even if -its -use as prog-nos-tic indi-ca-tor of cere-bral -high--grade gli-o-mas is -still debat-ed. (minervamedica.it)
- In rela-tion to -the -index, -patients -have -been divid-ed -into 3 -groups, -with dif-fer-ent sur-vi-val -rates: L) Ki-67 LI ≤10%, -with -mean sur-vi-val -rate of 30.7 -months, M) -from 10.1% to 30%, -with -mean sur-vi-val -rate of 15.8 -months, -and H) >30%, -with -mean sur-vi-val -rate of 20.2 -months. (minervamedica.it)
- Dubois S, Guyétant S, Menei P, Rodien P, Illouz F, Vielle B, Rohmer V (2007) Relevance of Ki-67 and prognostic factors for recurrence/progression of gonadotropic adenomas after first surgery. (springer.com)
- Synthetic peptide from the human Ki-67 allantigen. (genetex.com)
- The relationship between residual tumor growth and Ki-67 Li exceeding the cutoff value of 2.2 % was significant ( p = 0.01 in univariate, p = 0.044 in multivariate analysis). (springer.com)