AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesTechnology, Industry, AgricultureInformation Science
NocodazoleMicrotubulesTubulin ModulatorsColchicineTubulinDemecolcineCytochalasin DMitosisSpindle ApparatusPaclitaxelBenzimidazolesBrefeldin ACytoskeletonCytochalasinsGolgi ApparatusSalamandridaeMicrotubule-Associated ProteinsMetaphaseKinetochoresCell CycleCell LineMad2 ProteinsCentrosomeHeLa CellsPrometaphaseFluorescent Antibody TechniqueActinsAneugensCytochalasin BMicroscopy, FluorescencePolyploidyAlkaloidsG2 PhaseCarbamatesAnaphasePodophyllotoxinCells, CulturedAntineoplastic AgentsMicroscopy, VideoCyclin B1VinblastineActin CytoskeletonDyneinsMicroscopy, ElectronMonensinCyclin BCell Cycle ProteinsCell MembraneNucleic Acid Synthesis InhibitorsTelophaseCDC2 Protein KinaseCyclopentanesThiazolidinesCentriolesProtein TransportEndocytosis
Nocodazole
Nocodazole is an antineoplastic agent which exerts its effect by depolymerizing microtubules.
Microtubules
Slender, cylindrical filaments found in the cytoskeleton of plant and animal cells. They are composed of the protein TUBULIN and are influenced by TUBULIN MODULATORS.
Tubulin Modulators
Agents that interact with TUBULIN to inhibit or promote polymerization of MICROTUBULES.
Colchicine
A major alkaloid from Colchicum autumnale L. and found also in other Colchicum species. Its primary therapeutic use is in the treatment of gout, but it has been used also in the therapy of familial Mediterranean fever (PERIODIC DISEASE).
Tubulin
A microtubule subunit protein found in large quantities in mammalian brain. It has also been isolated from SPERM FLAGELLUM; CILIA; and other sources. Structurally, the protein is a dimer with a molecular weight of approximately 120,000 and a sedimentation coefficient of 5.8S. It binds to COLCHICINE; VINCRISTINE; and VINBLASTINE.
Demecolcine
An alkaloid isolated from Colchicum autumnale L. and used as an antineoplastic.
Cytochalasin D
A fungal metabolite that blocks cytoplasmic cleavage by blocking formation of contractile microfilament structures resulting in multinucleated cell formation, reversible inhibition of cell movement, and the induction of cellular extrusion. Additional reported effects include the inhibition of actin polymerization, DNA synthesis, sperm motility, glucose transport, thyroid secretion, and growth hormone release.
Mitosis
A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.
Spindle Apparatus
A microtubule structure that forms during CELL DIVISION. It consists of two SPINDLE POLES, and sets of MICROTUBULES that may include the astral microtubules, the polar microtubules, and the kinetochore microtubules.
Paclitaxel
A cyclodecane isolated from the bark of the Pacific yew tree, TAXUS BREVIFOLIA. It stabilizes MICROTUBULES in their polymerized form leading to cell death.
Benzimidazoles
Compounds with a BENZENE fused to IMIDAZOLES.
Brefeldin A
A fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity.
Cytoskeleton
The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.
Cytochalasins
11- to 14-membered macrocyclic lactones with a fused isoindolone. Members with INDOLES attached at the C10 position are called chaetoglobosins. They are produced by various fungi. Some members interact with ACTIN and inhibit CYTOKINESIS.
Golgi Apparatus
A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Salamandridae
A family of Urodela consisting of 15 living genera and about 42 species and occurring in North America, Europe, Asia, and North Africa.
Microtubule-Associated Proteins
High molecular weight proteins found in the MICROTUBULES of the cytoskeletal system. Under certain conditions they are required for TUBULIN assembly into the microtubules and stabilize the assembled microtubules.
Metaphase
The phase of cell nucleus division following PROMETAPHASE, in which the CHROMOSOMES line up across the equatorial plane of the SPINDLE APPARATUS prior to separation.
Kinetochores
Large multiprotein complexes that bind the centromeres of the chromosomes to the microtubules of the mitotic spindle during metaphase in the cell cycle.
Cell Cycle
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
Cell Line
Established cell cultures that have the potential to propagate indefinitely.
Mad2 Proteins
Mad2 is a component of the spindle-assembly checkpoint apparatus. It binds to and inhibits the Cdc20 activator subunit of the anaphase-promoting complex, preventing the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Mad2 is required for proper microtubule capture at KINETOCHORES.
Centrosome
The cell center, consisting of a pair of CENTRIOLES surrounded by a cloud of amorphous material called the pericentriolar region. During interphase, the centrosome nucleates microtubule outgrowth. The centrosome duplicates and, during mitosis, separates to form the two poles of the mitotic spindle (MITOTIC SPINDLE APPARATUS).
HeLa Cells
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Prometaphase
The phase of cell nucleus division following PROPHASE, when the breakdown of the NUCLEAR ENVELOPE occurs and the MITOTIC SPINDLE APPARATUS enters the nuclear region and attaches to the KINETOCHORES.
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Actins
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
Aneugens
Agents which affect CELL DIVISION and the MITOTIC SPINDLE APPARATUS resulting in the loss or gain of whole CHROMOSOMES, thereby inducing an ANEUPLOIDY.
Cytochalasin B
A cytotoxic member of the CYTOCHALASINS.
Microscopy, Fluorescence
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
Polyploidy
The chromosomal constitution of a cell containing multiples of the normal number of CHROMOSOMES; includes triploidy (symbol: 3N), tetraploidy (symbol: 4N), etc.
Alkaloids
Organic nitrogenous bases. Many alkaloids of medical importance occur in the animal and vegetable kingdoms, and some have been synthesized. (Grant & Hackh's Chemical Dictionary, 5th ed)
G2 Phase
The period of the CELL CYCLE following DNA synthesis (S PHASE) and preceding M PHASE (cell division phase). The CHROMOSOMES are tetraploid in this point.
Carbamates
Derivatives of carbamic acid, H2NC(=O)OH. Included under this heading are N-substituted and O-substituted carbamic acids. In general carbamate esters are referred to as urethanes, and polymers that include repeating units of carbamate are referred to as POLYURETHANES. Note however that polyurethanes are derived from the polymerization of ISOCYANATES and the singular term URETHANE refers to the ethyl ester of carbamic acid.
Anaphase
The phase of cell nucleus division following METAPHASE, in which the CHROMATIDS separate and migrate to opposite poles of the spindle.
Podophyllotoxin
A lignan (LIGNANS) found in PODOPHYLLIN resin from the roots of PODOPHYLLUM plants. It is a potent spindle poison, toxic if taken internally, and has been used as a cathartic. It is very irritating to skin and mucous membranes, has keratolytic actions, has been used to treat warts and keratoses, and may have antineoplastic properties, as do some of its congeners and derivatives.
Cells, Cultured
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Antineoplastic Agents
Substances that inhibit or prevent the proliferation of NEOPLASMS.
Microscopy, Video
Microscopy in which television cameras are used to brighten magnified images that are otherwise too dark to be seen with the naked eye. It is used frequently in TELEPATHOLOGY.
Cyclin B1
A cyclin B subtype that colocalizes with MICROTUBULES during INTERPHASE and is transported into the CELL NUCLEUS at the end of the G2 PHASE.
Vinblastine
Antitumor alkaloid isolated from Vinca rosea. (Merck, 11th ed.)
Actin Cytoskeleton
Fibers composed of MICROFILAMENT PROTEINS, which are predominately ACTIN. They are the smallest of the cytoskeletal filaments.
Dyneins
A family of multisubunit cytoskeletal motor proteins that use the energy of ATP hydrolysis to power a variety of cellular functions. Dyneins fall into two major classes based upon structural and functional criteria.
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Monensin
An antiprotozoal agent produced by Streptomyces cinnamonensis. It exerts its effect during the development of first-generation trophozoites into first-generation schizonts within the intestinal epithelial cells. It does not interfere with hosts' development of acquired immunity to the majority of coccidial species. Monensin is a sodium and proton selective ionophore and is widely used as such in biochemical studies.
Cyclin B
A cyclin subtype that is transported into the CELL NUCLEUS at the end of the G2 PHASE. It stimulates the G2/M phase transition by activating CDC2 PROTEIN KINASE.
Cell Cycle Proteins
Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS.
Cell Membrane
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Nucleic Acid Synthesis Inhibitors
Compounds that inhibit cell production of DNA or RNA.
Telophase
The final phase of cell nucleus division following ANAPHASE, in which two daughter nuclei are formed, the CYTOPLASM completes division, and the CHROMOSOMES lose their distinctness and are transformed into CHROMATIN threads.
CDC2 Protein Kinase
Phosphoprotein with protein kinase activity that functions in the G2/M phase transition of the CELL CYCLE. It is the catalytic subunit of the MATURATION-PROMOTING FACTOR and complexes with both CYCLIN A and CYCLIN B in mammalian cells. The maximal activity of cyclin-dependent kinase 1 is achieved when it is fully dephosphorylated.
Cyclopentanes
A group of alicyclic hydrocarbons with the general formula R-C5H9.
Thiazolidines
Reduced (protonated) form of THIAZOLES. They can be oxidized to THIAZOLIDINEDIONES.
Centrioles
Self-replicating, short, fibrous, rod-shaped organelles. Each centriole is a short cylinder containing nine pairs of peripheral microtubules, arranged so as to form the wall of the cylinder.
Protein Transport
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
Endocytosis
Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.

EB1, a protein which interacts with the APC tumour suppressor, is associated with the microtubule cytoskeleton throughout the cell cycle. (1/1483)

The characteristics of the adenomatous polyposis coli (APC) associated protein EB1 were examined in mammalian cells. By immunocytochemistry EB1 was shown to be closely associated with the microtubule cytoskeleton throughout the cell cycle. In interphase cells EB1 was associated with microtubules along their full length but was often particularly concentrated at their tips. During early mitosis, EB1 was localized to separating centrosomes and associated microtubules, while at metaphase it was associated with the spindle poles and associated microtubules. During cytokinesis EB1 was strongly associated with the midbody microtubules. Treatment with nocodazole caused a diffuse redistribution of EB1 immunoreactivity, whereas treatment with cytochalasin D had no effect. Interestingly, treatment with taxol abolished the EB1 association with microtubules. In nocodazole washout experiments EB1 rapidly became associated with the centrosome and repolymerizing microtubules. In taxol wash-out experiments EB1 rapidly re-associated with the microtubule cytoskeleton, resembling untreated control cells within 10 min. Immunostaining of SW480 cells, which contain truncated APC incapable of interaction with EB1, showed that the association of EB1 with microtubules throughout the cell cycle was not dependent upon an interaction with APC. These results suggest a role for EB1 in the control of microtubule dynamics in mammalian cells.  (+info)

Microtubule-dependent plus- and minus end-directed motilities are competing processes for nuclear targeting of adenovirus. (2/1483)

Adenovirus (Ad) enters target cells by receptor-mediated endocytosis, escapes to the cytosol, and then delivers its DNA genome into the nucleus. Here we analyzed the trafficking of fluorophore-tagged viruses in HeLa and TC7 cells by time-lapse microscopy. Our results show that native or taxol-stabilized microtubules (MTs) support alternating minus- and plus end-directed movements of cytosolic virus with elementary speeds up to 2.6 micrometer/s. No directed movement was observed in nocodazole-treated cells. Switching between plus- and minus end-directed elementary speeds at frequencies up to 1 Hz was observed in the periphery and near the MT organizing center (MTOC) after recovery from nocodazole treatment. MT-dependent motilities allowed virus accumulation near the MTOC at population speeds of 1-10 micrometer/min, depending on the cell type. Overexpression of p50/dynamitin, which is known to affect dynein-dependent minus end-directed vesicular transport, significantly reduced the extent and the frequency of minus end-directed migration of cytosolic virus, and increased the frequency, but not the extent of plus end-directed motility. The data imply that a single cytosolic Ad particle engages with two types of MT-dependent motor activities, the minus end- directed cytoplasmic dynein and an unknown plus end- directed activity.  (+info)

CLIP-170 highlights growing microtubule ends in vivo. (3/1483)

A chimera with the green fluorescent protein (GFP) has been constructed to visualize the dynamic properties of the endosome-microtubule linker protein CLIP170 (GFP-CLIP170). GFP-CLIP170 binds in stretches along a subset of microtubule ends. These fluorescent stretches appear to move with the growing tips of microtubules at 0.15-0.4 microm/s, comparable to microtubule elongation in vivo. Analysis of speckles along dynamic GFP-CLIP170 stretches suggests that CLIP170 treadmills on growing microtubule ends, rather than being continuously transported toward these ends. Drugs affecting microtubule dynamics rapidly inhibit movement of GFP-CLIP170 dashes. We propose that GFP-CLIP170 highlights growing microtubule ends by specifically recognizing the structure of a segment of newly polymerized tubulin.  (+info)

Distribution of 5-chloromethylfluorescein diacetate staining during meiotic maturation and fertilization in vitro of mouse oocytes. (4/1483)

The aim of this confocal microscopy study was to determine whether the pattern of CellTracker Green 5-chloromethylfluorescein diacetate (CMFDA) staining changes during meiotic maturation and fertilization in vitro of mouse oocytes. At different times during meiotic maturation and fertilization, oocytes, zygotes and two-cell embryos were stained with CMFDA to demonstrate intracellular glutathione S-transferase activity. After washing in CMFDA-free medium, most oocytes, zygotes and embryos were stained with dihydroethidium (HE) to visualize DNA structures. Meiotic maturation and fertilization in vitro of mouse oocytes were associated with changes in the pattern of intracellular CMFDA staining. In particular, accumulations of CMFDA-positive membranes were observed around the nucleus of germinal vesicle (GV) oocytes, overlaying the sperm nucleus as well as overlaying the first mitotic spindle if this approached the plasma membrane. Staining of oocytes and zygotes with the probes 3,3'-dihexyloxacarbocyanine iodine [DiOC6(3)], which stains all the intracellular membranes, and rhodamine 123, which stains active mitochondria, demonstrated that the intracellular structures evidenced by CMFDA staining did not correspond to accumulations of mitochondria. Exposure of oocytes and zygotes to the microtubule-disrupting agent nocodazole or the actin-depolymerizing drug cytochalasin D revealed an autonomous microfilament-dependent transport and relocation of CMFDA-positive membranes during meiotic maturation and fertilization. Such a transport of CMFDA-positive membranes may be envisaged as a protective shield built to prevent damage to DNA from endogenous and exogenous mutagen metabolites.  (+info)

Binding of Gal4p and bicoid to nucleosomal sites in yeast in the absence of replication. (5/1483)

The yeast transcriptional activator Gal4p can bind to sites in nucleosomal DNA in vivo which it is unable to access in vitro. One event which could allow proteins to bind to otherwise inaccessible sites in chromatin in living cells is DNA replication. To determine whether replication is required for Gal4p to bind to nucleosomal sites in yeast, we have used previously characterized chromatin reporters in which Gal4p binding sites are incorporated into nucleosomes. We find that Gal4p is able to perturb nucleosome positioning via nucleosomal binding sites in yeast arrested either in G1, with alpha-factor, or in G2/M, with nocodazole. Similar results were obtained whether Gal4p synthesis was induced from the endogenous promoter by growth in galactose medium or by an artificial, hormone-inducible system. We also examined binding of the Drosophila transcriptional activator Bicoid, which belongs to the homeodomain class of transcription factors. We show that Bicoid, like Gal4p, can bind to nucleosomal sites in SWI+ and swi1Delta yeast and in the absence of replication. Our results indicate that some feature of the intracellular environment other than DNA replication or the SWI-SNF complex permits factor access to nucleosomal sites.  (+info)

Intracellular trafficking pathways in the assembly of connexins into gap junctions. (6/1483)

Trafficking pathways underlying the assembly of connexins into gap junctions were examined using living COS-7 cells expressing a range of connexin-aequorin (Cx-Aeq) chimeras. By measuring the chemiluminescence of the aequorin fusion partner, the translocation of oligomerized connexins from intracellular stores to the plasma membrane was shown to occur at different rates that depended on the connexin isoform. Treatment of COS-7 cells expressing Cx32-Aeq and Cx43-Aeq with brefeldin A inhibited the movement of these chimera to the plasma membrane by 84 +/- 4 and 88 +/- 4%, respectively. Nocodazole treatment of the cells expressing Cx32-Aeq and Cx43-Aeq produced 29 +/- 16 and 4 +/- 7% inhibition, respectively. In contrast, the transport of Cx26 to the plasma membrane, studied using a construct (Cx26/43T-Aeq) in which the short cytoplasmic carboxyl-terminal tail of Cx26 was replaced with the extended carboxyl terminus of Cx43, was inhibited 89 +/- 5% by nocodazole and was minimally affected by exposure of cells to brefeldin A (17 +/-11%). The transfer of Lucifer yellow across gap junctions between cells expressing wild-type Cx32, Cx43, and the corresponding Cx32-Aeq and Cx43-Aeq chimeras was reduced by nocodazole treatment and abolished by brefeldin A treatment. However, the extent of dye coupling between cells expressing wild-type Cx26 or the Cx26/43T-Aeq chimeras was not significantly affected by brefeldin A treatment, but after nocodazole treatment, transfer of dye to neighboring cells was greatly reduced. These contrasting effects of brefeldin A and nocodazole on the trafficking properties and intercellular dye transfer are interpreted to suggest that two pathways contribute to the routing of connexins to the gap junction.  (+info)

The growth-related, translationally controlled protein P23 has properties of a tubulin binding protein and associates transiently with microtubules during the cell cycle. (7/1483)

The translationally controlled protein P23 was discovered by the early induction of its rate of synthesis after mitogenic stimulation of mouse fibroblasts. P23 is expressed in almost all mammalian tissues and it is highly conserved between animals, plants and yeast. Based on its amino acid sequence, P23 cannot be attributed to any known protein family, and its cellular function remains to be elucidated. Here, we present evidence that P23 has properties of a tubulin binding protein that associates with microtubules in a cell cycle-dependent manner. (1) P23 is a cytoplasmic protein that occurs in complexes of 100-150 kDa, and part of P23 can be immunoprecipitated from HeLa cell extracts with anti-tubulin antibodies. (2) In immunolocalisation experiments we find P23 associated with microtubules during G1, S, G2 and early M phase of the cell cycle. At metaphase, P23 is also bound to the mitotic spindle, and it is detached from the spindle during metaphase-anaphase transition. (3) A GST-P23 fusion protein interacts with alpha- and beta-tubulin, and recombinant P23 binds to taxol-stabilised microtubules in vitro. The tubulin binding domain of P23 was identified by mutational analysis; it shows similarity to part of the tubulin binding domain of the microtubule-associated protein MAP-1B. (4) Overexpression of P23 results in cell growth retardation and in alterations of cell morphology. Moreover, elevation of P23 levels leads to microtubule rearrangements and to an increase in microtubule mass and stability.  (+info)

Golgi structure correlates with transitional endoplasmic reticulum organization in Pichia pastoris and Saccharomyces cerevisiae. (8/1483)

Golgi stacks are often located near sites of "transitional ER" (tER), where COPII transport vesicles are produced. This juxtaposition may indicate that Golgi cisternae form at tER sites. To explore this idea, we examined two budding yeasts: Pichia pastoris, which has coherent Golgi stacks, and Saccharomyces cerevisiae, which has a dispersed Golgi. tER structures in the two yeasts were visualized using fusions between green fluorescent protein and COPII coat proteins. We also determined the localization of Sec12p, an ER membrane protein that initiates the COPII vesicle assembly pathway. In P. pastoris, Golgi stacks are adjacent to discrete tER sites that contain COPII coat proteins as well as Sec12p. This arrangement of the tER-Golgi system is independent of microtubules. In S. cerevisiae, COPII vesicles appear to be present throughout the cytoplasm and Sec12p is distributed throughout the ER, indicating that COPII vesicles bud from the entire ER network. We propose that P. pastoris has discrete tER sites and therefore generates coherent Golgi stacks, whereas S. cerevisiae has a delocalized tER and therefore generates a dispersed Golgi. These findings open the way for a molecular genetic analysis of tER sites.  (+info)

Dose-dependent effect of nocodazole on endothelial cell cytoskeleton<...
TY - JOUR. T1 - Dose-dependent effect of nocodazole on endothelial cell cytoskeleton. AU - Smurova, K. M.. AU - Birukova, A. A.. AU - Verin, A. D.. AU - Alieva, Irina B.. PY - 2008/6/1. Y1 - 2008/6/1. N2 - The endothelium lining the inner surface of blood vessels fulfils an important barrier function and specifically, it controls vascular membrane permeability as well as nutrient and metabolite exchange in circulating blood and tissue fluids. Disturbances in vascular endothelium barrier function (vascular endothelium dysfunction) are coupled to cytoskeleton rearrangements, actomyosin contractility, and as a consequence, formation of paracellular gaps between endothelial cells. Microtubules constitute the first effector link in the reaction cascade resulting in vascular endothelium dysfunction. Increased vascular permeability associated with many human diseases is also manifested as a side effect in anticancer mitosis-blocking therapy. The aim of this study was to examine the possibility of ...
https://augusta.pure.elsevier.com/en/publications/dose-dependent-effect-of-nocodazole-on-endothelial-cell-cytoskele
The role of microtubules in neutrophil polarity and migration in live zebrafish | Journal of Cell ScienceThe role of microtubules in neutrophil polarity and migration in live zebrafish | Journal of Cell Science
Here we investigated microtubule dynamics and function during neutrophil migration in vivo. In accordance with reported in vitro findings, we found that microtubule disruption impairs neutrophil directed migration to wounds but induces cell motility and polarity in a PI(3)K-independent manner. We also found that Rho mediates nocodazole-induced neutrophil motility. These findings are consistent with previous findings in vitro, but further provide a physiological context for neutrophil migration. In addition, we elucidated two previously unidentified roles of microtubules during neutrophil motility in vivo. First, microtubule arrays nucleate in front of the nucleus and mainly radiate towards the uropod. Second, Rac is activated by microtubule depolymerization in primary human neutrophils and is necessary for nocodazole-induced neutrophil motility in vivo.. Leukocytes are often described as having the microtubule cytoskeleton and MTOC behind the nucleus at the uropod (Friedl and Weigelin, 2008; ...
http://jcs.biologists.org/content/125/23/5702
CLIP-170 staining after nocodazole treatment. CLIP-170 | Open-iCLIP-170 staining after nocodazole treatment. CLIP-170 | Open-i
CLIP-170 staining after nocodazole treatment. CLIP-170 appears in green and chromosomes in red. 10-15 optical sections were merged for the final images. Oocyt
https://openi.nlm.nih.gov/detailedresult.php?img=PMC2199729_JCB9812023.f9&req=4
MAP Kinase Is Required for the Spindle Assembly Checkpoint but Is Dispensable for the Normal M Phase Entry and Exit in Xenopus...MAP Kinase Is Required for the Spindle Assembly Checkpoint but Is Dispensable for the Normal M Phase Entry and Exit in Xenopus...
A previous report has shown that microtubule depolymerization by nocodazole treatment arrests cell cycle extracts in M phase if containing very high densities of sperm nuclei. The use of MKP-1 further suggested that MAP kinase, the most likely target of MKP-1, may have a role in the spindle assembly checkpoint (20). However, whether classical MAP kinase is involved in the mitotic arrest remains to be determined. To address this question, we used MAP kinase-depleted extracts. We monitored the level of MPF as histone H1 kinase activity in the extracts supplemented with 9,000 sperm nuclei/μl. In the absence of nocodazole, the extracts returned to interphase after the first M phase, irrespective of whether MAP kinase was depleted or not (Fig. 3,A). In the presence of nocodazole, mock-treated extracts were arrested at the first M phase and the high MPF activity was sustained (Fig. 3,B, mock), whereas MAP kinase-depleted extracts returned to interphase after the first M phase (Fig. 3,B, αMAPK). In ...
https://rupress.org/jcb/article/136/5/1091/573/MAP-Kinase-Is-Required-for-the-Spindle-Assembly
Sport stars are not role models anymoreSport stars are not role models anymore
Lasers have made the 21st century even more technologically advanced with the are not role models, implementation at the cellular level. How To. Confocal microscopy alternatively to ultraviolent mercury lamps, are higher in wavelength and are more detailed in illumination in regards to shapes of sport stars role anymore, organelles (Katula). In this experiment, using confocal microscopy, two organelles were visualized to history understand the drug effect of nocodazole on NIH3T3 cells. Using two fluorescent stains, Mitotracker Red (accumulates in mitochondria) and role Hoechst 33342 (accumulates in nuclei) were used to test what microtubules would look like resulting from the drug. Nocodazole is a drug that penetrates the microtubules to where it can be synchronized. Ap World Essay 2007. (Karbowski). Microtubules are made up of polarized tubulin that are important for cytoskeletal rigidity, mitotic division, and act as a branch in sport role models anymore transporting molecules to other parts ...
http://carpetrent.com/topic/essays-2016175229/
A genetic screen identifies BRCA2 and PALB2 as key regulators of G2 checkpoint maintenance | EMBO ReportsA genetic screen identifies BRCA2 and PALB2 as key regulators of G2 checkpoint maintenance | EMBO Reports
Figure 1. High‐throughput siRNA screen identifies kinases that regulate the ionizing‐radiation‐induced G2 checkpoint. (A) Schematic overview of the robot‐automated high‐throughput siRNA screen. p53 dominant‐negative U2OS (U2OS p53dneg) cells were reverse transfected and, after incubation for 2 days, treated with 6 Gy of IR and, 2 h later, with nocodazole for 8 h and subsequently stained with H3Ser10p antibody to stain mitotic cells, and Hoechst to stain the nuclei. (B) Flow cytometric analysis of U2OS p53dneg cells. Where indicated, cells were treated with IR (6 Gy) and 2 h later with nocodazole for 8 h. Cells were collected and stained with phospho H3ser10 (H3Ser10p) antibody and propidium iodide (PI). (C) CHK1 depletion abrogates the G2 checkpoint. U2OS p53dneg cells were transfected with CHK1 siRNA and treated with IR (6 Gy) and 2 h later with nocodazole for 8 h. Top panel: immunofluorescence microscopy images obtained from the robot‐automated screen. Bottom panel: cells were ...
http://embor.embopress.org/content/12/7/705
Effect of nocodazole on vesicular traffic to the apical and basolateral surfaces of polarized MDCK cells.Effect of nocodazole on vesicular traffic to the apical and basolateral surfaces of polarized MDCK cells.
Author(s): Breitfeld, PP; McKinnon, WC; Mostov, KE | Abstract: A polarized cell, to maintain distinct basolateral and apical membrane domains, must tightly regulate vesicular traffic terminating at either membrane domain. In this study we have examined the extent to which microtubules regulate such traffic in polarized cells. Using the polymeric immunoglobulin receptor expressed in polarized MDCK cells, we have examined the effects of nocodazole, a microtubule-disrupting agent, on three pathways that deliver proteins to the apical surface and two pathways that deliver proteins to the basolateral surface. The biosynthetic and transcytotic pathways to the apical surface are dramatically altered by nocodazole in that a portion of the protein traffic on each of these two pathways is misdirected to the basolateral surface. The apical recycling pathway is slowed in the presence of nocodazole but targeting is not disrupted. In contrast, the biosynthetic and recycling pathways to the basolateral surface are
https://escholarship.org/uc/item/6ht8741s
Tubulin-specific chaperone ETubulin-specific chaperone E
Hypoparathyroidism, mental retardation and facial dysmorphism (HRD) is a fatal developmental disease caused by mutations in tubulin-specific chaperone E (TBCE). A mouse Tbce mutation causes progressive motor neuronopathy. To dissect the functions of TBCE and the pathogenesis of HRD, mutations were generated in Drosophila tbce, and its expression was manipulated in a tissue-specific manner. Drosophila tbce nulls are embryonic lethal. Tissue-specific knockdown and overexpression of tbce in the neuromusculature resulted in disrupted and increased microtubules, respectively. Alterations in TBCE expression also affected neuromuscular synapses. Genetic analyses revealed an antagonistic interaction between TBCE and the microtubule-severing protein Spastin. Moreover, treatment of muscles with the microtubule-depolymerizing drug nocodazole implicated TBCE as a tubulin polymerizing protein. Taken together, these results demonstrate that TBCE is required for the normal development and function of ...
http://www.sdbonline.org/sites/fly/genebrief/tbce_tubulinchaperone.htm
Phosphorylation regulates targeting of cytoplasmic dynein to kinetochores during mitosis | JCBPhosphorylation regulates targeting of cytoplasmic dynein to kinetochores during mitosis | JCB
The contributions of MT attachment and kinetochore tension in the SAC are still under investigation (Nicklas et al., 1995; Waters et al., 1998; King et al., 2000; Shannon et al., 2002; Pinsky and Biggins, 2005), so the importance of tension was tested using brief treatment with taxol. This approach maintains MT attachment but reduces tension at the kinetochores of aligned chromosomes (Waters et al., 1998). Interkinetochore distance measurements confirmed decreased tension but not the completely relaxed kinetochore state observed in nocodazole (Fig. S4). PT89 signal was retained at the kinetochores of taxol-treated cells (Fig. 6 A) at levels similar to nocodazole-treated cells. As in nocodazole-treated cells, the retention of PT89 was linked to the retention of BubR1. Together, these results suggest that effective dynein dephosphorylation requires both MT attachment and kinetochore tension.. The requirement for both MT attachment and tension was reminiscent of previous work on kinetochore ...
http://jcb.rupress.org/content/183/5/819
Development of porcine embryos reconstituted with somatic cells and enucleated metaphase I and II oocytes matured in a protein...Development of porcine embryos reconstituted with somatic cells and enucleated metaphase I and II oocytes matured in a protein...
Abstract Background Many cloned animals have been created by transfer of differentiated cells at G0/G1 or M phase of the cell cycle into enucleated M II oocytes having high maturation/meiosis/mitosis-promoting factor activity. Because maturation/meiosis/mitosis-promoting factor activity during oocyte maturation is maximal at both M I and M II, M I oocytes may reprogram differentiated cell nuclei as well. The present study was conducted to examine the developmental ability in vitro of porcine embryos reconstructed by transferring somatic cells (ear fibroblasts) into enucleated M I or M II oocytes. Results Analysis of the cell cycle stages revealed that 91.2 ± 0.2% of confluent cells were at the G0/G1 phase and 54.1 ± 4.4% of nocodazole-treated cells were at the G2/M phase, respectively. At 6 h after activation, nuclear swelling was observed in 50.0-88.9% and 34.4-39.5% of embryos reconstituted with confluent cells and nocodazole-treated cells regardless of the recipient oocytes, ...
https://athenaeum.libs.uga.edu/handle/10724/19833
Live-cell monitoring of tyrosine phosphorylation in focal adhesions following microtubule disruption | Journal of Cell ScienceLive-cell monitoring of tyrosine phosphorylation in focal adhesions following microtubule disruption | Journal of Cell Science
Tyrosine phosphorylation is implicated in the formation, maintenance and turnover of cell-matrix adhesions (Barry and Critchley, 1994; Ridley and Hall, 1994; Chrzanowska-Wodnicka and Burridge, 1994; Bershadsky et al., 1996; Retta et al., 1996; Ayalon and Geiger, 1997; Schneider et al., 1998), yet the mechanism underlying this involvement is still obscure. In this study, we addressed this issue by comparing local changes in PY levels to the recruitment of several FA proteins induced by the microtubule-disrupting drug nocodazole. For this purpose, we have combined quantitative immunofluorescence microscopy with a novel approach for monitoring kinetics of tyrosine phosphorylation in live cells.. FA assembly is a multistage process that involves the transformation of small, dot-like focal complexes into large FAs (see Geiger et al., 2001). Previous studies have shown that application of mechanical force to focal complexes, either by increasing cytoskeletal contractility or by external perturbation, ...
https://jcs.biologists.org/content/116/6/975?ijkey=a7ce20a3540403f6782961e891964522c2d441ea&keytype2=tf_ipsecsha
Mechanism of cell death in Burkitt lymphomasMechanism of cell death in Burkitt lymphomas
Apoptosis resistance is the major cause of chemotherapy failure in most kinds of cancers, including Burkitt lymphomas (BL). To elucidate molecular mechanisms regulating the development of apoptosis resistance, a panel of 15 BL cell lines was investigated for apoptosis induction upon treatment with microtubule inhibitors taxol, nocodazole and vincristine. Significant differences were observed in the extent of apoptosis induction among BL cell lines examined. Interestingly, cell lines exhibiting resistance to taxol- or nocodazole-induced apoptosis, showed development of polyploidy (,4N) and vice versa, displaying an inverse relationship between apoptosis and polyploidy induction. Further, in sensitive cell lines taxol-induced apoptosis was accompanied by caspase activation, Bid cleavage and Mcl-1 down-regulation. In contrast, most apoptosis resistant cell lines exhibited a loss of Bax and Bak expression and showed prolonged mitotic arrest with ,4N DNA content upon treatment. To gain mechanistic ...
https://edoc.hu-berlin.de/handle/18452/16738
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We hypothesized that the effects of Centrin2 inactivation could be associated with transient microtubule disruption. In support of this idea, we found that the treatment of freshly isolated E16 mouse neurons with the microtubule-destabilizing agent nocodazole (6 μm, 2 h) lead to disruption of the cytoplasmic polarity, as evidenced by the dispersion of the Golgi apparatus and late endosomes (see supplemental Fig. 3C,D, available at www.jneurosci.org as supplemental material) (n = 90 cells from 3 brains) as well as neurite retraction (data not shown) (de Anda et al., 2005). To confirm the specificity of the Centrin2 inactivation using CALI, we imaged neurons in irradiated slices that did not express the Centrin2-KR and found normal neuronal differentiation with the polarized cytoplasm oriented toward the medial/apical neurite (Fig. 3C,D) (8 h) that would become the axon (Fig. 3D, 42 h, black arrowheads and Inset 1) (for the whole time-lapse sequence and detailed z-stack analysis of the L1 ...
http://www.jneurosci.org/content/30/31/10391
Move it or lose it: axis specification in Xenopus | DevelopmentMove it or lose it: axis specification in Xenopus | Development
Initially, MTs in the vegetal shear zone are disorganized and short, but as cortical rotation progresses, they lengthen into parallel bundles, which have their plus-ends oriented away from the site of sperm entry (Fig. 2C,F) (Houliston and Elinson, 1991a). The subcortical MTs depolymerize near the end of the first cell cycle, ending the process of cortical rotation (Schroeder and Gard, 1992; Marrari et al., 2003). During rotation, the cortex (and the dorsalizing activity) moves towards the plus-ends of the MTs, explaining why the direction of cortical rotation depends on the site of fertilization and why the dorsal organizer forms on the side of the embryo opposite the sperm entry point (reviewed by Houliston and Elinson, 1992).. The proper formation of the MT array is crucial for the development of the dorsal axis. If MT polymerization is blocked during the first cell cycle by exposure to UV irradiation or to the MT-depolymerizing drug nocodazole, or with inhibitory antibodies to the ...
https://dev.biologists.org/content/131/15/3491
Stabilization of microtubules through the expression of | Open-iStabilization of microtubules through the expression of | Open-i
Stabilization of microtubules through the expression of Syk. (A)MDA-MB-231 cells lacking Syk (−Syk) or expressing Syk (+Syk)and treated with nocodazole were s
https://openi.nlm.nih.gov/detailedresult.php?img=PMC4295795_bi-2014-00325n_0001&req=4
Recognizing Chromosomes in Trouble: Association of the Spindle Checkpo by Oliver Kerscher, Luciana B. Crotti et al.Recognizing Chromosomes in Trouble: Association of the Spindle Checkpo by Oliver Kerscher, Luciana B. Crotti et al.
Spindle checkpoint proteins monitor the interaction of the spindle apparatus with the kinetochores, halting anaphase even if the microtubule attachment of only a single chromosome is altered. In this study, we show that Bub3p of Saccharomyces cerevisiae, an evolutionarily conserved spindle checkpoint protein, exhibits distinct interactions with an altered or defective kinetochore(s). We show for the first time that green fluorescent protein-tagged S. cerevisiae Bub3p (Bub3-GFP) exhibits not only a diffuse nuclear localization pattern but also forms distinct nuclear foci in unperturbed growing and G2/M-arrested cells. As Bub3-GFP foci overlap only a subset of kinetochores, we tested a model in which alterations or defects in kinetochore or spindle integrity lead to the distinct enrichment of Bub3p at these structures. In support of our model, kinetochore-associated Bub3-GFP is enriched upon activation of the spindle checkpoint due to nocodazole-induced spindle disassembly, overexpression of the
https://publish.wm.edu/aspubs/19/
A centrosomal protein FOR20 regulates microtubule assembly dynamics and plays a role in cell migration | Biochemical JournalA centrosomal protein FOR20 regulates microtubule assembly dynamics and plays a role in cell migration | Biochemical Journal
Here, we report that a centrosomal protein FOR20 [FOP (FGFR1 (fibroblast growth factor receptor 1) oncogene protein)-like protein of molecular mass of 20 kDa; also named as C16orf63, FLJ31153 or PHSECRG2] can regulate the assembly and stability of microtubules. Both FOR20 IgG antibody and GST (glutathione S-transferase)-tagged FOR20 could precipitate tubulin from the HeLa cell extract, indicating a possible interaction between FOR20 and tubulin. FOR20 was also detected in goat brain tissue extract and it cycled with microtubule-associated proteins. Furthermore, FOR20 bound to purified tubulin and inhibited the assembly of tubulin in vitro. The overexpression of FOR20 depolymerized interphase microtubules and the depletion of FOR20 prevented nocodazole-induced depolymerization of microtubules in HeLa cells. In addition, the depletion of FOR20 suppressed the dynamics of individual microtubules in live HeLa cells. FOR20-depleted MDA-MB-231 cells displayed zigzag motion and migrated at a slower rate ...
http://www.biochemj.org/content/474/16/2841
Visualizing Mutation-Specific Differences in the Trafficking-Deficient by Allison R. Hall, Corey L. Anderson et al.Visualizing Mutation-Specific Differences in the Trafficking-Deficient by Allison R. Hall, Corey L. Anderson et al.
KCNH2 encodes the Kv11.1 α-subunit that underlies the rapidly activating delayed-rectifier K+ current in the heart. Loss-of-function KCNH2 mutations cause long QT syndrome type 2 (LQT2), and most LQT2-linked missense mutations inhibit the trafficking of Kv11.1 channel protein to the cell surface membrane. Several trafficking-deficient LQT2 mutations (e.g., G601S) generate Kv11.1 proteins that are sequestered in a microtubule-dependent quality control (QC) compartment in the transitional endoplasmic reticulum (ER). We tested the hypothesis that the QC mechanisms that regulate LQT2-linked Kv11.1 protein trafficking are mutation-specific. Confocal imaging analyses of HEK293 cells stably expressing the trafficking-deficient LQT2 mutation F805C showed that, unlike G601S-Kv11.1 protein, F805C-Kv11.1 protein was concentrated in several transitional ER subcompartments. The microtubule depolymerizing drug nocodazole differentially affected G601S- and F805C-Kv11.1 protein immunostaining. Nocodazole caused G601S
https://uknowledge.uky.edu/physiology_facpub/121/
The microtubule-depolymerizing agent ansamitocin P3 programs dendritic cells toward enhanced anti-tumor immunityThe microtubule-depolymerizing agent ansamitocin P3 programs dendritic cells toward enhanced anti-tumor immunity
In addition to direct tumor cell cytotoxicity, chemotherapy can mediate tumor reduction through immune modulation of the tumor microenvironment to promote anti-tumor immunity. Mature dendritic cells (DCs) play key roles in priming robust immune responses in tumor-bearing hosts. Here, we screened a p …
https://pubmed.ncbi.nlm.nih.gov/24906866/?dopt=Abstract
order Nocodazoleorder Nocodazole
Analysis and theory development in cognitive psychology and science education study remain mainly isolated. AP biology programs in high school and accomplished a score of 4 or 5 5 on the AP Biology examination. These college students participated as part of their normal classroom activities. The nonmajors group (= 68) was composed of students enrolled …Read More. ...
http://researchhunt.com/tag/order-nocodazole/
Enhanced tumor cell killing by ultrasound after microtubule depolymerization - Fingerprint - UTMB Health Research Expert...Enhanced tumor cell killing by ultrasound after microtubule depolymerization - Fingerprint - UTMB Health Research Expert...
Powered by Pure, Scopus & Elsevier Fingerprint Engine™ © 2021 Elsevier B.V We use cookies to help provide and enhance our service and tailor content. By continuing you agree to the use of cookies. ...
https://researchexperts.utmb.edu/en/publications/enhanced-tumor-cell-killing-by-ultrasound-after-microtubule-depol/fingerprints/
CIL:17448, Homo sapiens, cervical carcinoma. CIL. DatasetCIL:17448, Homo sapiens, cervical carcinoma. CIL. Dataset
Fluorescence images of control and Si-RNA transfected HeLa cells treated with nocodazole and allowed to recover. Mitotic cells were stained for alpha-...
http://cellimagelibrary.org/images/17448
The role of microfilaments and microtubules in apical growth and dimorphism of Candida albicans | Microbiology SocietyThe role of microfilaments and microtubules in apical growth and dimorphism of Candida albicans | Microbiology Society
Cytoskeleton inhibitors were used to study morphogenesis in the pathogenic and dimorphic fungus Candida albicans Nocodazole is a specific microtubule inhibitor and chloropropham (CIPC), at high concentrations, is an inhibitor of microtubules and microfilaments. Distribution of microtubules and microfilaments was studied by immunofluorescence techniques using anti-tubulin antibody with FITC-conjugated secondary antibody, and by staining with Rh-phalloidin. Nocodazole did not arrest apical cell elongation at a concentration (20 µg ml−1) that inhibited nuclear division and migration. Cytoplasmic and nuclear microtubules disappeared within 30 min in filamentous cells under these conditions. However, the Rh-phalloidin-stained actin granules which were localized in the tips of filamentous cells, and the microfilaments, were arranged normally at this concentration of nocodazole. Growth, and normal distribution of microtubules and microfilaments, were inhibited by a high concentration (200 µg ml−1) of
https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-136-6-1067
Type 1 protein phosphatase acts in opposition to IpL1 protein kinase in regulating yeast chromosome segregation. | Molecular...Type 1 protein phosphatase acts in opposition to IpL1 protein kinase in regulating yeast chromosome segregation. | Molecular...
The IPL1 gene is required for high-fidelity chromosome segregation in the budding yeast Saccharomyces cerevisiae. Conditional ipl1ts mutants missegregate chromosomes severely at 37 degrees C. Here, we report that IPL1 encodes an essential putative protein kinase whose function is required during the later part of each cell cycle. At 26 degrees C, the permissive growth temperature, ipl1 mutant cells are defective in the recovery from a transient G2/M-phase arrest caused by the antimicrotubule drug nocodazole. In an effort to identify additional gene products that participate with the Ipl1 protein kinase in regulating chromosome segregation in yeast, a truncated version of the previously identified DIS2S1/GLC7 gene was isolated as a dosage-dependent suppressor of ipl1ts mutations. DIS2S1/GLC7 is predicted to encode a catalytic subunit (PP1C) of type 1 protein phosphatase. Overexpression of the full-length DIS2S1/GLC7 gene results in chromosome missegregation in wild-type cells and exacerbates the ...
https://mcb.asm.org/content/14/7/4731?ijkey=15bde2785944937fd898c3da1f81395f125f79b8&keytype2=tf_ipsecsha
Cortical and cytoplasmic flow polarity in early embryonic cells of Caenorhabditis elegans. | Journal of Cell Biology |...Cortical and cytoplasmic flow polarity in early embryonic cells of Caenorhabditis elegans. | Journal of Cell Biology |...
We have examined the cortex of Caenorhabditis elegans eggs during pseudocleavage (PC), a period of the first cell cycle which is important for the generation of asymmetry at first cleavage (Strome, S. 1989. Int. Rev. Cytol. 114: 81-123). We have found that directed, actin dependent, cytoplasmic, and cortical flow occurs during this period coincident with a rearrangement of the cortical actin cytoskeleton (Strome, S. 1986. J. Cell Biol. 103: 2241-2252). The flow velocity (4-7 microns/min) is similar to previously determined particle movements driven by cortical actin flows in motile cells. We show that directed flows occur in one of the daughters of the first division that itself divides asymmetrically, but not in its sister that divides symmetrically. The cortical and cytoplasmic events of PC can be mimicked in other cells during cytokinesis by displacing the mitotic apparatus with the microtubule polymerization inhibitor nocodazole. In all cases, the polarity of the resulting cortical and ...
https://rupress.org/jcb/article/121/6/1343/59451/Cortical-and-cytoplasmic-flow-polarity-in-early
Polewards chromosome movement driven by microtubule depolymerization in vitro.
We constructed complexes between isolated chromosomes and microtubules made from purified tubulin to study the movement of chromosomes towards the minus end of microtubules in vitro, a process analogous to the movement of chromosomes towards the pole of the spindle at anaphase of mitosis. Our results show that the energy for this movement is derived solely from microtubule depolymerization, and indicate that anaphase movement of chromosomes is both powered and regulated by microtubule depolymerization at the kinetochore ...
http://vivo.mblwhoilibrary.org/display/publication43883
Posttranslational modifications of alpha tubulin: detyrosination and acetylation differentiate populations of interphase...Posttranslational modifications of alpha tubulin: detyrosination and acetylation differentiate populations of interphase...
Subsets of microtubules enriched in posttranslationally detyrosinated (Gundersen, G. G., M. H. Kalnoski, and J. C. Bulinski. 1984. Cell. 38:779) or acetylated (Piperno, G., M. Le Dizet, and X. Chang. 1987. J. Cell Biol. 104:298), alpha tubulin have previously been described in interphase cultured cells. In this study an immunofluorescence comparison of these minor populations of microtubules revealed that, in African green monkey kidney epithelial cells (TC-7 line), the population of microtubules enriched in detyrosinated tubulin was virtually coincident with the population enriched in acetylated alpha tubulin. In some cell types, however, such as human HeLa or marsupial PtK-2 cells, only one posttranslationally modified form of tubulin, i.e., acetylated or detyrosinated, respectively, was detectable in microtubules. In TC-7 cells, although both modifications were present, dissimilar patterns and kinetics of reappearance of microtubules enriched in detyrosinated and acetylated tubulin were ...
http://jcb.rupress.org/content/106/4/1213
CiNii 論文 - Mitochondria use actin filaments as rails for fast translocation in Arabidopsis...
Although mitochondria are known to move actively in plant cells, little is known about how they move. In higher plants, actin filaments have been reported to be involved in the movement of organelles, such as chloroplasts, peroxisomes, endoplasmic reticulum and Golgi apparatus. Mitochondria were visualized in living ,i,Arabidopsis thaliana,/i, plants using fluorescent proteins fused to a mitochondria targeting signal. To compare the movement of mitochondria to the movements of another organelle, we also examined peroxisomes because of their similarity in size. Velocities of individual mitochondria and peroxisomes, as measured by time-lapse laser scanning microscopy, varied, although the average velocities of the two organelles were similar. Latrunculin B, an actin-depolymerizing drug, stopped movement of mitochondria and peroxisomes, demonstrating that movement of these organelles depends on actin filaments. On the other hand, propyzamide, a microtubule-depolymerizing drug, did not affect the ...
https://ci.nii.ac.jp/naid/10021912042
Phosphoregulation of Rad51/Rad52 by CDK1 functions as a molecular switch for cell cycle-specific activation of homologous...Phosphoregulation of Rad51/Rad52 by CDK1 functions as a molecular switch for cell cycle-specific activation of homologous...
Similar to that described in previous studies (6, 7), our examination into cell cycle-dependent regulation of homologous recombination was undertaken by α-factor treatment to induce G1 arrest and nocodazole treatment to induce G2/M arrest. It has been reported that activation of homologous recombination is also observed in the S phase in S. cerevisiae (1, 20). Because replication stress is a major source of DSBs, DNA damage repair by homologous recombination is critically required to maintain genome stability in the S phase. Thus, it is reasonable to assume that a mechanism exists whereby homologous recombination is also activated in the S phase. In this study, we found that the phosphorylation of Rad51 and Rad52 for the progression of homologous recombination is catalyzed by Cdc28 combined with either Clb2 or Clb3. Given that Clb2 and Clb3 are redundantly expressed in the S and G2/M phases (fig. S2D), it is presumable that Cdc28 combined with either Clb2 or Clb3 induces Rad51 and Rad52 to ...
https://advances.sciencemag.org/content/6/6/eaay2669?rss=1
How rotenone destroys the dopamine neurons
Based on these findings, we believe that microtubules are a critical target of PD environmental toxins such as rotenone, said Feng. Since many microtubule-depolymerizing agents are compounds naturally produced in many plants, our research points to the need to examine their possible link to Parkinsons disease. In addition, PD has a higher incidence in rural areas and is associated with pesticides and insecticides frequently used in farming practices ...
http://www.rxpgnews.com/rotenone/How_rotenone_destroys_the_dopamine_neurons_2152_printer.shtml
Novel mechanistic link between microtubule disruption and inhibition of tumor angiogenesisNovel mechanistic link between microtubule disruption and inhibition of tumor angiogenesis
El nostre treball demostra que el 2ME2 inhibeix langiogènesis tumoral mitjançant la inhibició dels nivells proteics del factor HIF-1 així com la seva activitat transcripcional, incloent la transcripció del VEGF, en diverses línies cel.lulars. El 2ME2 no indueix la degradació proteica a través del proteosoma ni tampoc redueix els nivells dARNm del HIF-1, sinó que més aviat inhibeix la síntesi proteica de novo. Notablement, hem mostrat que el 2ME2 inhibeix el factor HIF-1 amb posterioritat a la disrupció del citoesquelet de microtúbuls aportant sòlides proves que afavoreixen una relació funcional entre els efectes anti-angiogènics i la disrupció dels microtúbuls. Aquests efectes no són una propietat única del 2ME2 sinó que és compartida per altres agents que actuen sobre els microtúbuls, com ara el taxol i la vincristina, suggerint un mecanisme dacció semblant per a tots aquests agents. Finalment, la nostra investigació demostra per primera vegada que el 2ME2 ...
http://www.tdx.cat/handle/10803/4458
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Immunocompromisation is a major cause of opportunistic infections, activation of latent viruses, and the development of certain carcinomas such as Kaposis sarcoma (1, 2). This is particularly evident in many diseases that weaken the immune surveillance system, including AIDS and various cancer treatment modalities (e.g., radiation therapy, chemotherapy, and, in particular, antimicrotubule therapy), primarily because of severe myelosuppression and immunosuppression (3, 4). Finally, the resolution of these infections is dependent on the treatment modality of the malignancy and the ability of the host to mount an adequate immune response to fight off the infection (2).. Although antimicrotubule drugs constitute an important major class of anticancer drugs mainly typified by agents that either overpolymerize or bundle microtubules (such as the taxane family, ref. 5) or those that depolymerize them (such as the vincas; ref. 6, recently reviewed in refs. 7, 8), these agents, due to their gross ...
http://mct.aacrjournals.org/content/6/11/2891
Anti-Tubulin 抗体 (ab56676) | アブカムAnti-Tubulin 抗体 (ab56676) | アブカム
マウス・モノクローナル抗体 ab56676 交差種: Rat,Rb,Hu 適用: WB,IHC-P,Flow Cyt…Tubulin抗体一覧…画像、プロトコール、文献などWeb上の情報が満載のアブカムの Antibody 製品。国内在庫と品質保証制度も充実。
http://www.abcam.co.jp/tubulin-antibody-ab56676.html
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The incidence and mortality of breast cancer have increased throughout the world (Althuis et al., 2005), and it remains the most common cancer in women (Murray et al., 2012). In recent years, more and more drugs explored in clinical trials have provided promising advances in breast cancer treatments. Microtubule-binding agents are among the efficacious chemotherapeutic drugs that are commonly used for the treatment of breast cancer. Although antimicrotubule drugs, such as PTX and docetaxel, have been successfully applied in the clinic, drug resistance often occurred (Kavallaris, 2010) and acted as an obstacle for first-line chemotherapy. Augmented efflux transporters and diverse β-tubulin isotypes were involved in the development of drug resistance (Dumontet and Sikic, 1999). Thus, there is a pressing need to develop new microtubule inhibitors that can escape from the efflux of transporters related to MDR.. As a promising microtubule inhibitor candidate, DPT possesses various pharmacologic ...
http://dmd.aspetjournals.org/content/46/5/542
Anti-Tubulin Antibody Products | Biocompare.comAnti-Tubulin Antibody Products | Biocompare.com
44 reviews. Compare Anti-Tubulin Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more.
https://www.biocompare.com/pfu/110447/soids/4626/Antibodies/Tubulin?rlpi_8764=2
AMPK activation promotes lipid droplet dispersion on detyrosinated microtubules to increase mitochondrial fatty acid oxidationAMPK activation promotes lipid droplet dispersion on detyrosinated microtubules to increase mitochondrial fatty acid oxidation
Lipid droplets (LDs) are intracellular organelles that provide fatty acids (FAs) to cellular processes including synthesis of membranes and production of metabolic energy. While known to move bidirectionally along microtubules (MTs), the role of LD motion and whether it facilitates interaction with other organelles are unclear. Here we show that during nutrient starvation, LDs and mitochondria relocate on detyrosinated MT from the cell centre to adopt a dispersed distribution. In the cell periphery, LD-mitochondria interactions increase and LDs efficiently supply FAs for mitochondrial beta-oxidation. This cellular adaptation requires the activation of the energy sensor AMPK, which in response to starvation simultaneously increases LD motion, reorganizes the network of detyrosinated MTs and activates mitochondria. In conclusion, we describe the existence of a specialized cellular network connecting the cellular energetic status and MT dynamics to coordinate the functioning of LDs and mitochondria ...
http://www.recercat.cat/handle/2072/302351
北京大学医学部机构知识库([email protected]): Embelin derivatives and their anticancer activity through microtubule disassembly
Biological activities of the 1,4-benzoquinone derivatives 5-O-ethylembelin (1) and 5-O-methylembelin (2) were investigated. Both of them showed anti proliferative activity against a panel of human tumor cell lines upon comparison to normal marsupial kidney cells (PtK2). They arrested HL-60 cells in the G(0)/G(1) phase of the cell cycle in a dose- and time-dependent manner. In HeLa cells, exposure to 100 mu M of 1 or 2 for 6 h induced a complete disassembly of the microtubule network and an increased number of cells blocked in mitotic stages. Treatment with 10 mu M of 1 and 2 for 24 h induced apoptosis in HL-60 cells. This evidence suggests that both 1 and 2 are promising novel antimitotic and anticancer molecules targeting microtubular proteins. ...
http://ir.bjmu.edu.cn/handle/400002259/62601
SID-530 | CAS# | microtubule inhibitor | MedKoo BiosciencesSID-530 | CAS# | microtubule inhibitor | MedKoo Biosciences
SID-530 is an intravenous formulation containing docetaxel, a semi-synthetic, second-generation taxane derived from a compound found in the European yew tree, Taxus baccata, with potential antineoplastic activity. Taxol analogue SID 530 binds to and stabilizes tubulin, inhibiting microtubule disassembly, which results in cell-cycle arrest at the G2/M phase and cell death. Check for active clinical trials or closed clinical trials using this agent. (NCI Thesaurus) (last updated: 6/23/2015)
http://www.medkoo.com/products/5838
SMCC-DM1 (DM1-SMCC) | Drug-Linker Conjugates for ADC | MedChemExpressSMCC-DM1 (DM1-SMCC) | Drug-Linker Conjugates for ADC | MedChemExpress
SMCC-DM1 (DM1-SMCC) is a drug-linker conjugate composed of a potent microtubule-disrupting agent DM1 and a linker SMCC to make antibody drug conjugate (ADC). - Mechanism of Action & Protocol.
https://www.medchemexpress.com/SMCC-DM1.html
Kinesin-8 motors act cooperatively to mediate length-dependent microtubule depolymerization • Research - Institut PasteurKinesin-8 motors act cooperatively to mediate length-dependent microtubule depolymerization • Research - Institut Pasteur
Motor proteins in the kinesin-8 family depolymerize microtubules in a length-dependent manner that may be crucial for controlling the length of organelles
https://research.pasteur.fr/en/publication/kinesin-8-motors-act-cooperatively-to-mediate-length-dependent-microtubule-depolymerization/
Browse our Dynamitin ELISA KitsBrowse our Dynamitin ELISA Kits
Order Dynamitin ELISA Kits for many Reactivities. Human, Mouse, Rat and more. Compare Dynamitin ELISA Kits and find the right product on antibodies-online.com.
http://www.antibodies-online.com/m-phase-pathway-68/dynamitin-elisa-kit-901/
JCI - G1 and G2 cell-cycle arrest following microtubule depolymerization in human breast cancer cellsJCI - G1 and G2 cell-cycle arrest following microtubule depolymerization in human breast cancer cells
for 8 hours (by this time most MCF-7 cells had not yet accumulated in mitosis and thus remained adherent), fixed, stained with anti-α-tubulin, and visualized by fluorescence microscopy ...
https://sdjiaheng.net.insight.mobile.jci.org/articles/view/13275/figure/6
Tubulin-tirozinska ligaza - Википедија, слободна енциклопедијаTubulin-tirozinska ligaza - Википедија, слободна енциклопедија
Rudiger, M., Wehland, J., Weber, K. (1994). „The carboxy-terminal peptide of detyrosinated α tubulin provides a minimal system to study the substrate specificity of tubulin-tyrosine ligase. Eur. J. Biochem. 220: 309-320. PMID 7510228 ...
https://sr.wikipedia.org/wiki/Tubulin-tirozinska_ligaza
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It is well known that c-Jun N-terminal kinase (JNK) plays pivotal roles in various mitotic events, but its function in mammalian oocyte meiosis remains unknown. In this study, we found that no specific JNK2 signal was detected in germinal vesicle stage. JNK2 was associated with the spindles especially the spindle poles and cytoplasmic microtubule organizing centers at prometaphase I, metaphase I, and metaphase II stages. JNK2 became diffusely distributed and associated with the midbody at telophase I stage. Injection of myc-tagged JNK2α1 mRNA into oocytes also revealed its localization on spindle poles. The association of JNK2 with spindle poles was further confirmed by colocalization with the centrosomal proteins, γ-tubulin and Plk1. Nocodazole treatment showed that JNK2 may interact with Plk1 to regulate the spindle assembly. Then we investigated the possible function of JNK2 by JNK2 antibody microinjection and JNK specific inhibitor SP600125 treatment. These two manipulations caused ...
http://core-cms.prod.aop.cambridge.org/core/search?filters%5BauthorTerms%5D=Hai-Xin%20Bo&eventCode=SE-AU
Plus itPlus it
Eribulin mesylate (eribulin), an analogue of the marine natural product halichondrin B, is a microtubule-depolymerizing drug that has utility in the treatment of patients with breast cancer. Clinical trial results have demonstrated that eribulin treatment provides a survival advantage to patients with metastatic or locally advanced breast cancer previously treated with an anthracycline and a taxane. Furthermore, a pooled analysis of two pivotal phase III trials has demonstrated that eribulin also improves overall survival in several patient subgroups, including in women with HER2-negative disease and triple-negative breast cancer. This review covers the preclinical research that led to the clinical testing and approval of eribulin, as well as subsequent research that was prompted by distinct and unexpected effects of eribulin in the clinic. Initial studies with halichondrin B demonstrated unique effects on tubulin binding that resulted in distinct microtubule-dependent events and antitumor ...
http://clincancerres.aacrjournals.org/content/early/2015/05/07/1078-0432.CCR-14-3252
Examination of actin and microtubule dependent APC localisations in living mammalian cells | BMC Molecular and Cell Biology |...Examination of actin and microtubule dependent APC localisations in living mammalian cells | BMC Molecular and Cell Biology |...
The trafficking of the adenomatous polyposis coli (APC) tumour suppressor protein in mammalian cells is a perennially controversial topic. Immunostaining evidence for an actin-associated APC localisation at intercellular junctions has been previously presented, though live imaging of mammalian junctional APC has not been documented. Using live imaging of transfected COS-7 cells we observed intercellular junction-associated pools of GFP-APC in addition to previously documented microtubule-associated GFP-APC and a variety of minor localisations. Although both microtubule and junction-associated populations could co-exist within individual cells, they differed in their subcellular location, dynamic behaviour and sensitivity to cytoskeletal poisons. GFP-APC deletion mutant analysis indicated that a protein truncated immediately after the APC armadillo repeat domain retained the ability to localise to adhesive membranes in transfected cells. Supporting this, we also observed junctional APC immunostaining in
https://bmcmolcellbiol.biomedcentral.com/articles/10.1186/1471-2121-7-3/figures/6
Molecular characterisation of microtubule dependent organelle motility in the squid giant axon and optic lobe. :: Dartmouth...Molecular characterisation of microtubule dependent organelle motility in the squid giant axon and optic lobe. :: Dartmouth...
In Copyright. This material is protected by copyright and/or related rights. You are free to use this material in any way that is permitted by copyright law that applies to your use. For other uses, you need to obtain permission from the rights-holder(s). http://rightsstatements.org/page/InC/1.0/ ...
https://libarchive.dartmouth.edu/cdm/compoundobject/collection/dcdis/id/369446/rec/34
Pol κ: A DNA Polymerase Required for Sister Chromatid Cohesion | SciencePol κ: A DNA Polymerase Required for Sister Chromatid Cohesion | Science
To determine whether TRF function was required to maintain cohesion, we examined cohesion in the trf4-ts trf5double mutant. If TRF function were required to maintain cohesion, then cells arrested with nocodazole at a permissive temperature and then shifted to the nonpermissive temperature should show an increased cohesion defect. After nocodazole arrest at the permissive temperature of 30°C, the trf4-ts trf5double mutant was able to maintain cohesion as monitored by the GFP-marked chromosome assay. However, when mutant cells were shifted to 37°C for 3 hours, a marked increase in cohesion-defective cells was observed (Fig. 3B). This demonstrates that TRF function is indeed required to maintain cohesion.. The ability of cells lacking TRF function to duplicate the genome was then examined as follows. Synchronous populations of either wild-type or otherwise isogenic trf4-ts trf5double-mutant G1 daughter cells were obtained by centrifugal elutriation. Wild-type and mutant cells were then released ...
https://science.sciencemag.org/content/289/5480/774?ijkey=5c5585ae2c3bcbe35e790a929735e48f0cd27b1c&keytype2=tf_ipsecsha
4SC-207 | CAS#871015-11-1 | microtubule inhibitor | MedKoo Biosciences4SC-207 | CAS#871015-11-1 | microtubule inhibitor | MedKoo Biosciences
4SC-207 is a novel microtubule inhibitor, which shows strong anti-proliferative activity in a large panel of tumor cell lines with an average GI50 of 11 nM. In particular, 4SC-207 is active in multi-drug resistant cell lines, such as HCT-15 and ACHN, suggesting that it is a poor substrate for drug efflux pumps. 4SC-207 inhibits microtubule growth in vitro and in vivo and promotes, in a dose dependent manner, a mitotic delay/arrest, followed by apoptosis or aberrant divisions due to chromosome alignment defects and formation of multi-polar spindles. Furthermore, preliminary data from preclinical studies suggest low propensity towards bone marrow toxicities at concentrations that inhibit tumor growth in paclitaxel-resistant xenograft models. 4SC-207 may be a potential anti-cancer agent.
https://www.medkoo.com/products/5988
Organization of non-centrosomal microtubules in epithelial cells<...
TY - JOUR. T1 - Organization of non-centrosomal microtubules in epithelial cells. AU - Toya, Mika. AU - Takeichi, Masatoshi. PY - 2016/1/1. Y1 - 2016/1/1. N2 - Polarized epithelial cells contain a characteristic array of microtubules in which non-centrosomal microtubules are aligned along the apical-to-basal axis of the cell with their minus ends oriented towards the apical pole. Although this unique orientation of microtubules was discovered in the late 1980s, how this orientation is established remains unresolved partly because of limited information about molecular factors that regulate the minus ends of non-centrosomal microtubules. Recent studies, however, identified novel minus end- associated proteins, revealing mechanisms by which the polarized arrays of microtubules are established in epithelial cells. These studies have also demonstrated the importance of apico-basally orientated microtubules in intra-structural organization of cells. This review focuses on recent progress of our ...
https://waseda.pure.elsevier.com/en/publications/organization-of-non-centrosomal-microtubules-in-epithelial-cells
Cell structure and Microscopy - Revision Cards in A Level and IB BiologyCell structure and Microscopy - Revision Cards in A Level and IB Biology
The cytoskeleton is responsible for cell shape, motility (movement) of the cell as a whole, and motility of organelles within a cell. There are three types of filaments in the cytoplasm of most vertebrate cells: microfilaments, microtubules, and intermediate filaments.. filament systems are able to lengthen or shorten very rapidly. This dynamic nature of the cytoskeleton is necessary for cells to be able to change shape, complete cell division, or migrate.. The microfilament system is a network of filaments 6 nanometers (nm) in diameter that are important for anchoring plasma membrane proteins, for producing cell movement, and for cell division. The base filament is composed of a protein called actin.. Microtubules are the largest of the cytoskeletal filaments with a diameter of 25 nm. There are many parallels between the microfilament cytoskeletal system and the microtubule system. Like microfilaments, microtubules are produced by the self-assembly of a subunit, which in the case of ...
https://getrevising.co.uk/revision-cards/cell-structure-and-microscopy
Plus itPlus it
MLN0264 is an investigational antibody-drug conjugate (ADC) that consists of the human anti-guanylyl cyclase C (GCC) antibody linked to a microtubule-disrupting agent (monomethyl auristatin). As ADCs have a very long clearance half-life, the potential exists for a highly infrequent dosing schedule. A quantitative understanding of the relationship between exposure and preclinical antitumor biological activity is thus applied to support dose schedule selection in the clinic.. In this study, we develop a pharmacokinetic/efficacy (PK/E) relationship in xenograft models to evaluate the predictive contributions of exposure and xenograft characteristics to MLN0264 biological activity. Single dose pharmacokinetic (PK) data were obtained for a range of time points, and a linear two-compartment PK model was built. Xenograft biological activity studies were conducted in which MLN0264 was administered at various dose levels and dosing schedules to mice bearing one of six different xenograft models. We used ...
http://cancerres.aacrjournals.org/content/74/19_Supplement/4649
Main - Gremlin is a Key Pro-fibrogenic Factor in Chronic PancreatitisMain - Gremlin is a Key Pro-fibrogenic Factor in Chronic Pancreatitis
Mitotic spindle position defines the cell cleavage site during cytokinesis. cortex. In elongating cells asymmetrically, dynein-dependent spindle anchoring at the fixed cell cortex guarantees correct spindle setting. Our outcomes reveal the anaphase-specific spindle centering systems that obtain equal-sized cell department. syncytial embryos (Silverman-Gavrila et al., 2008). To evaluate the contribution of chromosome-derived Ran-GTP indicators in mammalian cells, we utilized tsBN2 cells, which include a heat range delicate mutation in RCC1 that stops the formation of Ran-GTP at the restricted heat range (Nishitani et al., 1991). In nocodazole treated tsBN2 cells, Anillin was decreased from the cell cortex in the location of the chromosome herd at the permissive heat range (Fig. 7A, still left) equivalent to HeLa cells (Fig. 6E). Nevertheless, at the restricted heat range, Anillin localised to the cell cortex also in the location of chromosomes (Fig. 7A, correct). The heat range change do not ...
http://bus2226w16.com/category/main/
Gremlin is a Key Pro-fibrogenic Factor in Chronic PancreatitisGremlin is a Key Pro-fibrogenic Factor in Chronic Pancreatitis
Mitotic spindle position defines the cell cleavage site during cytokinesis. cortex. In elongating cells asymmetrically, dynein-dependent spindle anchoring at the fixed cell cortex guarantees correct spindle setting. Our outcomes reveal the anaphase-specific spindle centering systems that obtain equal-sized cell department. syncytial embryos (Silverman-Gavrila et al., 2008). To evaluate the contribution of chromosome-derived Ran-GTP indicators in mammalian cells, we utilized tsBN2 cells, which include a heat range delicate mutation in RCC1 that stops the formation of Ran-GTP at the restricted heat range (Nishitani et al., 1991). In nocodazole treated tsBN2 cells, Anillin was decreased from the cell cortex in the location of the chromosome herd at the permissive heat range (Fig. 7A, still left) equivalent to HeLa cells (Fig. 6E). Nevertheless, at the restricted heat range, Anillin localised to the cell cortex also in the location of chromosomes (Fig. 7A, correct). The heat range change do not ...
http://bus2226w16.com/?document_srl=1443993
Pattern Analysis of Microtubule-Polymerizing and -Depolymerizing Agent by Carol A. Heckman, S. O. Uppal et al.Pattern Analysis of Microtubule-Polymerizing and -Depolymerizing Agent by Carol A. Heckman, S. O. Uppal et al.
Subcellular distribution of mass can be analyzed by a technique that involves culturing cells on interferometers and digitizing their interference contours. Contour sampling resulted in 102 variables per cell, which were predictors of oncogenic transformation. Cell phenotypes can be deconstructed by use of latent factors, which represent the covariance of the real variables. The reversal of the cancertype phenotype by a combination of microtubule- stabilizing and -depolymerizing agents was described previously. The implications of these results have been explored by clinicians who treated patients with the combination of docetaxel and vinorelbine (Navelbine®). The current study was performed to determine the effects of different combinations on phenotype and in phases of the cell cycle other than mitosis. Combinations of paclitaxel with either colchicine, podophyllotoxin, nocodazole, or vinblastine caused phenotype reversal. Paclitaxel analogue, 7-deoxytaxol, by itself caused reversal. Factors #4,
https://scholarworks.bgsu.edu/bio_sci_pub/8/
DNA repair and Chromatin remodeling genes
The table lists cell cycle regulated chromatin remodeling genes. Five transcriptome data sets, in which the genes that are periodically expressed in at least one data set, and one ribosomal profiling (translationally regulated) data set are included. Periodically expressed genes are indicated with cell cycle phase in which they show the highest expression level. Gene sets in which a given gene is not significantly cell cycle regulated is indicated as NR (not transcriptionally regulated in the cell cycle) or NTR (not translationally regulated in the cell cycle) in transcriptome and ribosomal profiling data sets, respectively. Four experiments are supplemented with expression profiles which can be found by clicking the gene name. Synchronization methods are abbreviated as: SS=serum starvation, TT= double thymidine block, NZ=nocodazole, MS=mitotic shake-off ...
http://dnarepairgenes.com/cc_chrommod.html
NocodazoleNocodazole
As nocodazole affects the cytoskeleton, it is often used in cell biology experiments as a control: for example, some dominant ... Cells treated with nocodazole arrest with a G2- or M-phase DNA content when analyzed by flow cytometry. Microscopy of ... Nocodazole is an antineoplastic agent which exerts its effect in cells by interfering with the polymerization of microtubules. ... Nocodazole has been shown to decrease the oncogenic potential of cancer cells via another microtubules-independent mechanisms. ...
https://en.wikipedia.org/wiki/Nocodazole
Induced cell cycle arrestInduced cell cycle arrest
Nocodazole is a chemical agent that interferes with the polymerization of microtubules. Cells treated with nocodazole arrest ... Taxol works in the opposite way of nocodazole, instead stabilizing the microtubule polymer and preventing it from disassembly. ... Xu K, Schwarz PM, Ludueña RF (Feb 2002). "Interaction of nocodazole with tubulin isotypes". Drug Development Research. 55 (2): ... Kuhn M (March 1998). "The microtubule depolymerizing drugs nocodazole and colchicine inhibit the uptake of Listeria ...
https://en.wikipedia.org/wiki/Induced_cell_cycle_arrest
FenbendazoleFenbendazole
Oxfendazole Nocodazole Praziquantel Plumb DC (2005). Plumb's veterinary drug handbook (Fifth ed.). Stockholm, Wis.: PhrmaVet. ...
https://en.wikipedia.org/wiki/Fenbendazole
PraziquantelPraziquantel
Fenbendazole Oxfendazole Nocodazole "Farnam Pet Press Release. TRUSTED D-WORM offers product for tapeworm management". Farnam ...
https://en.wikipedia.org/wiki/Praziquantel
Asymmetric cell divisionAsymmetric cell division
Experiments using nocodazole and taxol support this observation. Taxol, which stabilized microtubules, forced a significant ... Moreover, disruption of microtubule polymerization with nocodazole, and of actin polymerization with cytochalisin B, shows the ... Moreover, embryos treated with nocodazole, which sequesters tubulin dimers and promotes microtubule depolymerization, similarly ...
https://en.wikipedia.org/wiki/Asymmetric_cell_division
Cell synchronizationCell synchronization
Cells will remain arrested until the nocodazole has been washed out. Nocodazole does not appear to disrupt interphase ... "Production of large numbers of mitotic mammalian cells by use of the reversible microtubule inhibitor Nocodazole: Nocodazole ... Nocodazole is a rapidly-reversible inhibitor of microtubule polymerization that can be used to arrest cells before Anaphase at ... Because microtubules are vital in other cellular functions, sustained use of nocodazole can result in disruption of those ...
https://en.wikipedia.org/wiki/Cell_synchronization
PurinosomePurinosome
However, nocodazole also blocks formation of aggresomes, complicating interpretation of these observations. Partial inhibition ... Inhibition of microtubule polymerization with nocodazole blocks formation of the purinosome macrobodies, and reduces the flux ...
https://en.wikipedia.org/wiki/Purinosome
Cytoskeletal drugsCytoskeletal drugs
Vasquez RJ, Howell B, Yvon AM, Wadsworth P, Cassimeris L (June 1997). "Nanomolar concentrations of nocodazole alter microtubule ...
https://en.wikipedia.org/wiki/Cytoskeletal_drugs
MicrotubuleMicrotubule
Vinorelbine, Nocodazole, vincristine, and colchicine have the opposite effect, blocking the polymerization of tubulin into ... microtubule dynamics and lamellipodium formation revealed by direct imaging of microtubules in cells treated with nocodazole or ...
https://en.wikipedia.org/wiki/Microtubule
DRG2DRG2
2005). "Overexpression of DRG2 increases G2/M phase cells and decreases sensitivity to nocodazole-induced apoptosis". J. ...
https://en.wikipedia.org/wiki/DRG2
Synchronous cultureSynchronous culture
Nocodazole, for example, has been used in biological research for synchronization, although some evidence suggests it may lack ... 2006). "Nocodazole does not synchronize cells: implications for cell-cycle control and whole-culture synchronization" (PDF). ...
https://en.wikipedia.org/wiki/Synchronous_culture
VinblastineVinblastine
Microtubule-disruptive drugs like vinblastine, colcemid, and nocodazole have been reported to act by two mechanisms. At very ...
https://en.wikipedia.org/wiki/Vinblastine
Anterograde tracingAnterograde tracing
Effects of substances interacting with microtubular function and axonal flow [nocodazole, taxol and erythro-9-3-(2-hydroxynonyl ...
https://en.wikipedia.org/wiki/Anterograde_tracing
Aurora kinase CAurora kinase C
When cells overexpressing Aurora C were treated with nocodazole to turn on the SAC, Aurora B protein stability and activity ...
https://en.wikipedia.org/wiki/Aurora_kinase_C
Spindle checkpointSpindle checkpoint
Using drugs such as nocodazole and colchicine, the mitotic spindle disassembles and the cell cycle is blocked at the metaphase- ... Wang Y, Burke DJ (December 1995). "Checkpoint genes required to delay cell division in response to nocodazole respond to ... laevis meiosis II extracts without the addition of sperm of nuclei and nocodazole to prevent spindle assembly. The leading ...
https://en.wikipedia.org/wiki/Spindle_checkpoint
Stress granuleStress granule
This hypothesis is based on the fact that disruption of microtubules with the chemical nocodazole blocks the appearance of the ...
https://en.wikipedia.org/wiki/Stress_granule
Nuclear transferNuclear transfer
Microtubule inhibitors, such as nocodazole, are used to arrest the oocyte in M phase, during which its nuclear membrane is ...
https://en.wikipedia.org/wiki/Nuclear_transfer
HSPA1BHSPA1B
... proteins and is proteolytically activated in response to arachidonic acid and the microtubule-depolymerizing drug nocodazole". ...
https://en.wikipedia.org/wiki/HSPA1B
Mad2Mad2
In fact, when metaphase mammalian cells are treated with the spindle-depolymerizing agent nocodazole, Mad2 proteins become ... it was essential to execute a block in the metaphase-to-anaphase transition in response to the microtubule poison nocodazole. ...
https://en.wikipedia.org/wiki/Mad2
Control of chromosome duplicationControl of chromosome duplication
Evidence that CDKs regulate the localization of Mcm2-7 is that inactivation of CDKs in nocodazole arrested cells induced ...
https://en.wikipedia.org/wiki/Control_of_chromosome_duplication
Cingulin-like protein 1Cingulin-like protein 1
... cells and its localization at the apical junctional complex is perturbed by treatment with the microtubule drug nocodazole. ...
https://en.wikipedia.org/wiki/Cingulin-like_protein_1
HT-29HT-29
Accordingly, studies of HT-29 cells have shown induced differentation as a result of forskolin, Colchicine, nocodazole, and ...
https://en.wikipedia.org/wiki/HT-29
EpibolyEpiboly
Treating embryos with the microtubule depolymerizing agent nocodazole completely blocks epiboly of the YSL and partially blocks ...
https://en.wikipedia.org/wiki/Epiboly
List of MeSH codes (D03)List of MeSH codes (D03)
... nocodazole MeSH D03.438.103.675 - omeprazole MeSH D03.438.103.732 - pimozide MeSH D03.438.103.850 - thiabendazole MeSH D03.438. ...
https://en.wikipedia.org/wiki/List_of_MeSH_codes_(D03)
List of drugs: Nj-NzList of drugs: Nj-Nz
... nocodazole (INN) nofecainide (INN) nogalamycin (INN) Nogenic HC nolatrexed (INN) nolinium bromide (INN) nolpitantium besilate ( ...
https://en.wikipedia.org/wiki/List_of_drugs:_Nj–Nz
Telomeres in the cell cycleTelomeres in the cell cycle
They found that when the cells were released and concurrently treated with nocodazole, a G2/M phase cell cycle inhibitor, ...
https://en.wikipedia.org/wiki/Telomeres_in_the_cell_cycle
MAP6MAP6
They partially overlap the calmodulin binding domain and stabilize microtubules against both cold and nocodazole-induced ...
https://en.wikipedia.org/wiki/MAP6
https://www.selleckchem.com/products/nocodazole.ht..https://www.selleckchem.com/products/nocodazole.ht..
... www.selleckchem.com/products/nocodazole.html 5] kPa; after: 235.4 [163.5 : 349.9] kPa). Discomfort scores along with little ... https://www.selleckchem.com/pr....oducts/nocodazole.ht 5] kPa; after: 235.4 [163.5 : 349.9] kPa). Discomfort scores along with ...
https://uchatoo.com/post/208247_https-www-ttkan-co-novel-chapters-zuodaoqingtian-fenglingtianxia-https-www-ttkan.html
Podophyllotoxin and nocodazole counter the effect of IKP104 on tubulin decay<...Podophyllotoxin and nocodazole counter the effect of IKP104 on tubulin decay<...
... in the presence of nocodazole, IKP104 caused little or no stabilization of tubulin. Since colchicine, nocodazole, and ... in the presence of nocodazole, IKP104 caused little or no stabilization of tubulin. Since colchicine, nocodazole, and ... in the presence of nocodazole, IKP104 caused little or no stabilization of tubulin. Since colchicine, nocodazole, and ... in the presence of nocodazole, IKP104 caused little or no stabilization of tubulin. Since colchicine, nocodazole, and ...
https://scholars.uthscsa.edu/es/publications/podophyllotoxin-and-nocodazole-counter-the-effect-of-ikp104-on-tu
APC(FZR1) prevents nondisjunction in mouse oocytes by controlling meiotic spindle assembly timingAPC(FZR1) prevents nondisjunction in mouse oocytes by controlling meiotic spindle assembly timing
Nocodazole / pharmacology * Nondisjunction, Genetic* / drug effects * Nuclear Proteins / metabolism * Oocytes / cytology* * ...
https://pubmed.ncbi.nlm.nih.gov/22918942/
Plus itPlus it
The second set of nocodazole-treated cells was rinsed with nocodazole-free medium and cultured without nocodazole for 20 min at ... Because binding of nocodazole to tubulin is reversible, removal of nocodazole allows MT repolymerization, and as a result, a ... After 15 min of depolymerization in the presence of 2 μg/ml nocodazole, cells were rinsed and cultured for 20 min in nocodazole ... After 15 min of depolymerization in the presence of 2 μg/ml nocodazole, cells were rinsed and cultured for 20 min in nocodazole ...
https://www.jneurosci.org/content/34/5/1856
Microtubules tune mechanosensitive cell responses | Nature MaterialsMicrotubules tune mechanosensitive cell responses | Nature Materials
Then, 1 μM nocodazole was added and images were acquired every 2 min for 1 h. Acquisitions were performed with a Nikon Eclipse ... in cells treated with or without nocodazole. Scale bar (a, b, d, e, k, l): 10 μm; Number of independent experiments = 3 (for a- ... GFP-αTAT1 localization at FAs upon nocodazole treatment. Cells were transfected with GFP-αTAT1 and mCherry-vinculin. First, ...
https://www.nature.com/articles/s41563-021-01108-x?error=cookies_not_supported&code=04557934-8ef7-4888-bacc-933a5d1f5991
New ISO Standards for liquid handling instrumentsNew ISO Standards for liquid handling instruments
Watch this presentation by Dr. A. Bjoern Carle and Dr. George Rodrigues, on new standards for liquid handling
https://www.selectscience.net/SelectScience-TV/Videos/new-and-revised-iso-standards-for-manual-and-automated-liquid-handling-instruments/?videoID=5989
Frontiers | Immunoglobulin free light chains and GAGs mediate Multiple Myeloma Extracellular Vesicles uptake and secondary NfkB...Frontiers | Immunoglobulin free light chains and GAGs mediate Multiple Myeloma Extracellular Vesicles uptake and secondary NfkB...
C or with nocodazole 20 μM at 37°C followed by 4 h of exposure to EVs at 4°C or in the presence of nocodazole. Cells were then ... Furthermore, to block endocytosis cells were pre-incubated for 30 min at 4°C or with nocodazole 20 μM (Sigma) at 37°C. After ... Similar results were obtained after treating cells with the endocytosis inhibitor nocodazole (data not shown). It will be ... with nocodazole 20 μM. Internalization rate of PKH67 centrifuged 2 h at 100,000 × g without EVs. (100 Cells each experimental ...
https://www.frontiersin.org/articles/10.3389/fimmu.2014.00517/full
Tim Stearns' Profile | Stanford Profiles
Microtubules repolymerized randomly in the cytoplasm after nocodazole treatment and reversal. gamma-Tubulin dissociates from ... nocodazole. This phenotype is recessive for all alleles isolated. The CIN1, CIN2 and CIN4 genes were cloned by complementation ...
https://profiles.stanford.edu/tim-stearns
Rac1 activation can generate untemplated, lamellar membrane ruffles | BMC Biology | Full TextRac1 activation can generate untemplated, lamellar membrane ruffles | BMC Biology | Full Text
g Ruffle formation in nocodazole-treated cells (yellow arrows). Scale bar = 3 μm. h Ruffle lengths and widths show no ... Microtubule depolymerization was carried out by incubating the cells with 25 μM nocodazole for 2 h in DMEM and imaged in the ... 2f, Additional file 7: Movie S7). Inhibiting microtubule polymerization using nocodazole had no effect on the process of ... LLSM with media containing 25 μM nocodazole.. Microscopy. Cells were imaged using a lattice light-sheet microscope (3i, Denver ...
https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-021-00997-3
Antibody Purification (Affinity)
Nocodazole is one of anticancer drugs that synchronize cells at M-phase. It can interfere with the structure and function of ... Cell Synchronization with Nocodazole at Mitosis. Cell Synchronization is a process by which cells at different stages of the ...
http://www.abnova.com/abvideo/Antibody-Purification-Affinity.html
Profile | Biosciences | University of Exeter
Nocodazole stimulates RhoA activity, and inhibition of the RhoA target ROCK allows nocodazole-treated cells to re-establish ... Nocodazole stimulates RhoA activity, and inhibition of the RhoA target ROCK allows nocodazole-treated cells to re-establish ... METHODOLOGY/PRINCIPAL FINDINGS: Here we report that T cells treated with nocodazole to disrupt MTs are unable to form a stable ... METHODOLOGY/PRINCIPAL FINDINGS: Here we report that T cells treated with nocodazole to disrupt MTs are unable to form a stable ...
https://biosciences.exeter.ac.uk/staff/profile/index.php?web_id=aya_takesono
TRPV1 Agonist, Capsaicin, Induces Axon Outgrowth after Injury via Ca2+/PKA Signaling | eNeuroTRPV1 Agonist, Capsaicin, Induces Axon Outgrowth after Injury via Ca2+/PKA Signaling | eNeuro
... we used nocodazole as a positive control (Fig. 1A-D). Nocodazole controls were normalized to DMSO controls from the same plate ... Nocodazole consistently induced robust growth on each plate, giving a mean value of 3.9-fold over DMSO for the entire screen ( ... Nocodazole-treated wells were normalized to DMSO-treated wells from the same plate to control for plate-to-plate variability. ... If nocodazole failed to induce robust neurite outgrowth, all data from that plate were excluded, and those compounds were ...
https://www.eneuro.org/content/5/3/ENEURO.0095-18.2018?utm_source=EN&utm_medium=Webpage&utm_campaign=eneuro_blog
Polo-like Kinase 1 Inhibition as a Therapeutic Approach to Selectively Target BRCA1-Deficient Cancer Cells by Synthetic...Polo-like Kinase 1 Inhibition as a Therapeutic Approach to Selectively Target BRCA1-Deficient Cancer Cells by Synthetic...
Left, control of the M-phase arrest triggered by 10 ng/mL nocodazole. Statistical analysis was performed using two-way ANOVA ... Left, control of the M-phase arrest triggered by 10 ng/mL nocodazole. Statistical analysis was performed using two-way ANOVA ... Interestingly, experiments using the well-characterized mitotic poisons nocodazole and colcemide showed that these drugs also ... Dose-response experiment that shows the SL induction triggered by the mitotic poisons nocodazole and colcemide in BRCA1- ...
https://aacrjournals.org/clincancerres/article/25/13/4049/81880/Polo-like-Kinase-1-Inhibition-as-a-Therapeutic
Microtubules do not promote mitotic slippage when the spindle assembly checkpoint cannot be satisfied | Journal of Cell Biology...Microtubules do not promote mitotic slippage when the spindle assembly checkpoint cannot be satisfied | Journal of Cell Biology...
Unlike nocodazole or colcemid, vinblastine prevents MT assembly by binding to the β-tubulin subunit of the α/β-tubulin ... Video 4 is the same as video 1, except the culture was treated with 50 nM nocodazole and the framing rate was once every 10 min ... In our final study, we examined the kinetics of cyclin B/GFP degradation in RPE-1 cells treated with 50 nM nocodazole. If it ... As expected, spindle structure in mitotic RPE-1 cells fixed after 12-16 h in 50 nM nocodazole, 5 nM vinblastine, or 5 nM taxol ...
https://rupress.org/jcb/article/182/4/623/45392/Microtubules-do-not-promote-mitotic-slippage-when
In vitro comparison of liposomal drug delivery systems targeting the o | IJNIn vitro comparison of liposomal drug delivery systems targeting the o | IJN
Nocodazole was used to inhibit macropinocytosis, and cytochalasin D was used to inhibit both phagocytosis and macropinocytosis ... nocodazole (M1404), cytochalasin D (C8273), MTT-based assay kit (CGD1), and all buffer reagents were from Sigma-Aldrich Co. L- ... Nocodazole (pharmacological inhibitor of macropinocytosis) was unable to block the cellular uptake of both OTR-targeted ...
https://www.dovepress.com/in-vitro-comparison-of-liposomal-drug-delivery-systems-targeting-the-o-peer-reviewed-fulltext-article-IJN
SignalSilence® Bcl-xL siRNA I | Cell Signaling TechnologySignalSilence® Bcl-xL siRNA I | Cell Signaling Technology
... and nocodazole (4,5).. * Adams, J.M. and Cory, S. (1998) Science. 281, 1322-6. ...
https://www.cellsignal.com/products/sirna/bcl-xl-sirna-i/6362
CIL advanced searchCIL advanced search
A novel benzodiazepine derivative that suppresses microtubule dynamics and impairs mitotic progression | Journal of Cell...A novel benzodiazepine derivative that suppresses microtubule dynamics and impairs mitotic progression | Journal of Cell...
For comparison, we used in parallel 20 µM taxol and 10 µM nocodazole treatments (Fig. 4A; Fig. S1). Control neuroblasts formed ... The Mps1 inhibitor AZ3146 was from Calbiochem, the CDK1 inhibitor RO-3306 was from Merck, taxol and nocodazole were from Sigma- ... As expected, following prolonged mitotic arrest, neuroblasts treated with taxol or nocodazole underwent slippage (Fig. S1D,E). ... Whereas neuroblasts treated with taxol or nocodazole (which severely impair spindle assembly) remained arrested and underwent ...
https://journals.biologists.com/jcs/article/133/7/jcs239244/224866/A-novel-benzodiazepine-derivative-that-suppresses
Guttorm Alendal | Universitetet i BergenGuttorm Alendal | Universitetet i Bergen
Model to Assess Synergistic Mixture Effects between the Aromatic Hydrocarbon β-Naphthoflavone and the Azole Nocodazole on the ...
https://www.uib.no/personer/Guttorm.Alendal
PUB - Publikationen an der Universität Bielefeld
Non-invasive method to assess genotoxicity of nocodazole interfering with spindle formation in mammalian oocytes. Shen Y, ...
https://pub.uni-bielefeld.de/person/22132
NIOSHTIC-2 Search Results - Full View
Final Report Summary - HERACLES (Human cystic Echinococcosis ReseArch in CentraL and Eastern Societies) | FP7 | CORDIS |...Final Report Summary - HERACLES (Human cystic Echinococcosis ReseArch in CentraL and Eastern Societies) | FP7 | CORDIS |...
... nocodazole (NCZ), the use and process for preparation thereof". According to the present invention, therefore, new salts of the ...
https://cordis.europa.eu/project/rcn/110277/reporting/en?rcn=257759
Examination of the Chromosomes ofPolysphondylium pallidum Following Metaphase Arrest by Benzimidazole Derivatives and...Examination of the Chromosomes ofPolysphondylium pallidum Following Metaphase Arrest by Benzimidazole Derivatives and...
... ben late and nocodazole) caused the arrest of cell division, mitotic indices greater than 50%, chromosome doubling and lowered ... Welker D. L., Williams K. L. 1980; Mitotic arrest and chromosome doubling using thiabendazole, cambendazole, nocodazole and ben ... ben late and nocodazole) caused the arrest of cell division, mitotic indices greater than 50%, chromosome doubling and lowered ...
https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-116-2-409
Other - Generation of a selective small molecule inhibitor of the CBP/p300 bromodomain
We statement finding in Brazil in 9. among ticks in Uruguay (where Nocodazole tyrosianse inhibitor is the most common human- ... identical to the corresponding sequences of Nocodazole tyrosianse inhibitor Maculatum strain (GenBank accession nos. "type":" ... ":"U43802″U43802, respectively). The frequency of infection among ticks Nocodazole tyrosianse inhibitor examined within this ...
http://www.columbiagypsy.net/category/other/
MH DELETED MN ADDED MNMH DELETED MN ADDED MN
Nocodazole D3.438.103.637 D3.633.100.103.637 Nod Signaling Adaptor Proteins D12.644.360.24.307 D12.644.360.24.313 D12.776. ...
https://decs.bvs.br/E/mnchg2017.txt
ALG6 Phenotypes | SGDALG6 Phenotypes | SGD
... and is sensitive to HU and nocodazole; in large-scale studies, the null mutant requires myo-inositol, displays rounded cell ...
https://www.yeastgenome.org/locus/S000005528/phenotype
Untangling the Role of Tau in Alzheimers DiseaseUntangling the Role of Tau in Alzheimer's Disease
Importantly, the researchers found that a drug called nocodazole, which blocks new microtubule assembly, prevented injected tau ...
https://www.technologynetworks.com/neuroscience/news/untangling-the-role-of-tau-in-alzheimers-disease-362993
Combined effect of cell geometry and polarity domains determines the orientation of unequal division | eLifeCombined effect of cell geometry and polarity domains determines the orientation of unequal division | eLife
Embryo were placed in ASW with 15 µM of Cytochalasin B (Sigma-Aldrich, C6762), 10 µM Nocodazole (Sigma-Aldrich, M1414), and ... Embryos were treated with Cytochalasin B (15µM) and Nocodazole (10µM) from Nuclear Envelop Break Down to soften the cell cortex ... B-treated embryos were dependent on microtubules since they were completely abolished when embryos were treated with Nocodazole ... B-treated embryos were dependent on microtubules since they were completely abolished when embryos were treated with Nocodazole ...
https://elifesciences.org/articles/75639
Inhibition of RHO-kinase depends on factors that modify endothelial permeability<...
Under the suppression of Rho-kinase activity, unlike thrombin, nocodazole destroys both dynamic and stable microtubules. Thus, ... Under the suppression of Rho-kinase activity, unlike thrombin, nocodazole destroys both dynamic and stable microtubules. Thus, ... Under the suppression of Rho-kinase activity, unlike thrombin, nocodazole destroys both dynamic and stable microtubules. Thus, ... Under the suppression of Rho-kinase activity, unlike thrombin, nocodazole destroys both dynamic and stable microtubules. Thus, ...
https://augusta.pure.elsevier.com/en/publications/inhibition-of-rho-kinase-depends-on-factors-that-modify-endotheli
A Validated HPLC Method For The Determination Biology Essay | FreebookSummaryA Validated HPLC Method For The Determination Biology Essay | FreebookSummary
Nocodazole (NCD) is an anti-neoplastic agent which exerts its consequence in cells by interfering with the polymerisation of ... Nocodazole (NCD) is an anti-neoplastic agent which exerts its consequence in cells by interfering with the polymerisation of ... Determination of nocodazole in SLN. Aproximately 20 milligram of SLN suspension was assorted with DMSO to obtain a 10 ... Theriogenology 73: 429-436.Li D, Li P, Li G, Wang J, Wang E (2009) The consequence of nocodazole on the transfection efficiency ...
https://freebooksummary.com/a-validated-hplc-method-for-the-determination-biology-essay-essay
MicrotubulesPaclitaxelMitoticVinblastineColchicineTubulinProteinProteinsArrestStableCellsHttpsEffects
Microtubules4
  • Under the suppression of Rho-kinase activity, unlike thrombin, nocodazole destroys both dynamic and stable microtubules. (elsevier.com)
  • Nocodazole (NCD) is an anti-neoplastic agent which exerts its consequence in cells by interfering with the polymerisation of microtubules. (freebooksummary.com)
  • Nocodazole is an antineoplastic agent which exerts its effect by depolymerizing microtubules. (bvsalud.org)
  • the resistance of microtubules to nocodazole. (conferencedequebec.org)
Paclitaxel3
  • Bcl-xL is phosphorylated by JNK following treatment with microtubule-damaging agents, such as paclitaxel, vinblastine, and nocodazole (4,5). (cellsignal.com)
  • With regard to the predictive role of Aurora-A, it has been shown that its overexpression disrupts the spindle checkpoint activated by paclitaxel (Taxol) or nocodazole treatment, thus inducing the cells to become resistant to these drugs. (unipa.it)
  • The development therefore of small molecules with an Aurora-A inhibition function may make it possible to reduce or block the oncogenic activity of Aurora-A and in addition may improve the survival of oncological patients showing resistance to paclitaxel or nocodazole treatment. (unipa.it)
Mitotic1
  • Low concentrations of benzimidazole derivatives (thiabendazole, cambendazole, ben late and nocodazole) caused the arrest of cell division, mitotic indices greater than 50%, chromosome doubling and lowered plating efficiency. (microbiologyresearch.org)
Vinblastine1
  • At 37°C, the duration of mitosis in nocodazole, colcemid, or vinblastine concentrations that inhibit MT assembly varied from 20 to 30 h, revealing that different MT poisons differentially depress the cyclin B destruction rate during slippage. (rupress.org)
Colchicine2
  • To see if this is due to conformational effects specific to colchicine or simply to occupancy at the colchicine site, we examined the effects of nocodazole and podophyllotoxin, two well-known competitive inhibitors of colchicine for binding to tubulin, on IKP104's acceleration of decay. (uthscsa.edu)
  • Since colchicine, nocodazole, and podophyllotoxin have very different interactions with tubulin, but all inhibit the IKP104-induced enhancement of decay, our findings suggest that this inhibition arises from occupancy of the colchicine site rather than from a direct conformational effect of these two drugs. (uthscsa.edu)
Tubulin2
  • however, in the presence of nocodazole, IKP104 caused little or no stabilization of tubulin. (uthscsa.edu)
  • The studies suggested that 8j has a good binding affinity of −7.949 towards nocodazole binding site of tubulin while nocodazole has −7.462. (researcher-app.com)
Protein1
  • Total cell lysates (15 µg total protein) from HeLa cells untreated (-) or treated (+) with nocodazole (200 ng/mL, 16 hours) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL (1:1000 dilution) purified anti-STAT1 phospho (Ser727) antibody (clone A15158B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. (biolegend.com)
Proteins2
  • However, uropod-localized receptors and ezrin/radixin/moesin proteins still cluster in nocodazole-treated cells, indicating that MTs are required specifically for uropod stability. (exeter.ac.uk)
  • Noc, GLP-1 Receptor Proteins Formulation Nocodazole.Eur J Immunol. (gardos-channel.com)
Arrest1
  • 4th LMW-E-overexpressing cells neglect to arrest in the current presence of nocodazole. (immune-source.com)
Stable2
  • METHODOLOGY/PRINCIPAL FINDINGS: Here we report that T cells treated with nocodazole to disrupt MTs are unable to form a stable uropod or lamellipodium, and instead often move by membrane blebbing with reduced migratory persistence. (exeter.ac.uk)
  • Our analyses indicate that micronuclei in Kif18a deficient cells form stable nuclear envelopes characterized by (1) increased recruitment of nuclear envelope components, and (2) successful expansion of chromatin, which must decondense as cells exit mitosis, compared to the envelopes of micronuclei which form following nocodazole washout or radiation exposure. (uvm.edu)
Cells4
  • Nocodazole stimulates RhoA activity, and inhibition of the RhoA target ROCK allows nocodazole-treated cells to re-establish lamellipodia and uropods and persistent migratory polarity. (exeter.ac.uk)
  • REF-52 cells were treated with nocodazole to depolymerize MTs and induce collapse of most IFs to the cell center. (ucsd.edu)
  • Here we show that Slk19 is required for kinetochore clustering when yeast cells are treated with the microtubule-depolymerizing agent nocodazole. (omicsdi.org)
  • mutant cells exhibit delayed kinetochore capture and chromosome bipolar attachment after the disruption of the kinetochore-microtubule interaction by nocodazole, which is likely attributed to defective kinetochore clustering. (omicsdi.org)
Https1
  • https://www.selleckchem.com/products/nocodazole.ht. (uchatoo.com)
Effects1
  • New Conceptual Toxicokinetic Model to Assess Synergistic Mixture Effects between the Aromatic Hydrocarbon β-Naphthoflavone and the Azole Nocodazole on the CYP1A Biomarker in a Fish Cell Line. (uib.no)
Plus it
Plus it (jneurosci.org)
Microtubules tune mechanosensitive cell responses | Nature Materials
Microtubules tune mechanosensitive cell responses | Nature Materials (nature.com)
New ISO Standards for liquid handling instruments
New ISO Standards for liquid handling instruments (selectscience.net)
DMSO-based Sample Solutions from Artel for Low Volume Dispensing Verification | SelectScience
DMSO-based Sample Solutions from Artel for Low Volume Dispensing Verification | SelectScience (selectscience.net)
Tim Stearns' Profile | Stanford Profiles
Tim Stearns' Profile | Stanford Profiles (profiles.stanford.edu)
Rac1 activation can generate untemplated, lamellar membrane ruffles | BMC Biology | Full Text
Rac1 activation can generate untemplated, lamellar membrane ruffles | BMC Biology | Full Text (bmcbiol.biomedcentral.com)
Microtubules do not promote mitotic slippage when the spindle assembly checkpoint cannot be satisfied | Journal of Cell Biology...
Microtubules do not promote mitotic slippage when the spindle assembly checkpoint cannot be satisfied | Journal of Cell Biology... (rupress.org)
In vitro comparison of liposomal drug delivery systems targeting the o | IJN
In vitro comparison of liposomal drug delivery systems targeting the o | IJN (dovepress.com)
CIL advanced search
CIL advanced search (cellimagelibrary.org)
Absolute quantification of cohesin, CTCF and their regulators in human cells | eLife
Absolute quantification of cohesin, CTCF and their regulators in human cells | eLife (elifesciences.org)
A novel benzodiazepine derivative that suppresses microtubule dynamics and impairs mitotic progression | Journal of Cell...
A novel benzodiazepine derivative that suppresses microtubule dynamics and impairs mitotic progression | Journal of Cell... (journals.biologists.com)
PUB - Publikationen an der Universität Bielefeld
PUB - Publikationen an der Universität Bielefeld (pub.uni-bielefeld.de)
Examination of the Chromosomes ofPolysphondylium pallidum Following Metaphase Arrest by Benzimidazole Derivatives and...
Examination of the Chromosomes ofPolysphondylium pallidum Following Metaphase Arrest by Benzimidazole Derivatives and... (microbiologyresearch.org)
Conditional mutation of Smc5 in mouse embryonic stem cells perturbs condensin localization and mitotic progression | Journal of...
Conditional mutation of Smc5 in mouse embryonic stem cells perturbs condensin localization and mitotic progression | Journal of... (journals.biologists.com)
Podophyllotoxin and nocodazole counter the effect of IKP104 on tubulin decay<...
Podophyllotoxin and nocodazole counter the effect of IKP104 on tubulin decay<... (scholars.uthscsa.edu)
Inhibition of RHO-kinase depends on factors that modify endothelial permeability<...
Inhibition of RHO-kinase depends on factors that modify endothelial permeability<... (augusta.pure.elsevier.com)
A Validated HPLC Method For The Determination Biology Essay | FreebookSummary
A Validated HPLC Method For The Determination Biology Essay | FreebookSummary (freebooksummary.com)
Lilianna Solnica-Krezel - Research output - Research Profiles at Washington University School of Medicine
Lilianna Solnica-Krezel - Research output - Research Profiles at Washington University School of Medicine (profiles.wustl.edu)
Overexpression of the reduced molecular-weight isoforms (LMW-E) of cyclin E induces | immune-source.com
Overexpression of the reduced molecular-weight isoforms (LMW-E) of cyclin E induces | immune-source.com (immune-source.com)
PhosphoPair STAT1 Ser727 Antibody Set, STAT1, 10C4B40 A15158B
PhosphoPair STAT1 Ser727 Antibody Set, STAT1, 10C4B40 A15158B (biolegend.com)
The Human Homologue of Bub3 Is Required for Kinetochore Localization of Bub1 and a Mad3/Bub1-related Protein Kinase | Journal...
The Human Homologue of Bub3 Is Required for Kinetochore Localization of Bub1 and a Mad3/Bub1-related Protein Kinase | Journal... (rupress.org)
Bio2Vec
Bio2Vec (bio2vec.cbrc.kaust.edu.sa)
Wei-Hsin Chiu - 研究成果 - 國立成功大學
Wei-Hsin Chiu - 研究成果 - 國立成功大學 (researchoutput.ncku.edu.tw)
Raltitrexed | http://www.ck2inhibitor.com
Raltitrexed | http://www.ck2inhibitor.com (ck2inhibitor.com)
Genotype analysis of colorectal carcinomas through laser pressare catapulting (LPC)</em>...
Genotype analysis of colorectal carcinomas through laser pressare catapulting (LPC)</em>... (pure.unipa.it)