Nih 3t3 cells. Medical search

A continuous cell line of high contact-inhibition established from NIH Swiss mouse embryo cultures. The cells are useful for DNA transfection and transformation studies. (From ATCC [Internet]. Virginia: American Type Culture Collection; c2002 [cited 2002 Sept 26]. Available from http://www.atcc.org/)

Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.

An operating division of the US Department of Health and Human Services. It is concerned with the overall planning, promoting, and administering of programs pertaining to health and medical research. Until 1995, it was an agency of the United States PUBLIC HEALTH SERVICE.

Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.

Articles on conferences sponsored by NIH presenting summary statements representing the majority agreement of physicians, scientists, and other professionals convening for the purpose of reaching a consensus on a subject of interest. This heading is used for NIH consensus conferences as a means of scientific communication. In indexing it is viewed as a type of review article and as a tag for any article appearing in any publication of the NIH Office of Medical Applications of Research (OMAR).

Financial support of research activities.

The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.

Family of retrovirus-associated DNA sequences (ras) originally isolated from Harvey (H-ras, Ha-ras, rasH) and Kirsten (K-ras, Ki-ras, rasK) murine sarcoma viruses. Ras genes are widely conserved among animal species and sequences corresponding to both H-ras and K-ras genes have been detected in human, avian, murine, and non-vertebrate genomes. The closely related N-ras gene has been detected in human neuroblastoma and sarcoma cell lines. All genes of the family have a similar exon-intron structure and each encodes a p21 protein.

Genes whose gain-of-function alterations lead to NEOPLASTIC CELL TRANSFORMATION. They include, for example, genes for activators or stimulators of CELL PROLIFERATION such as growth factors, growth factor receptors, protein kinases, signal transducers, nuclear phosphoproteins, and transcription factors. A prefix of "v-" before oncogene symbols indicates oncogenes captured and transmitted by RETROVIRUSES; the prefix "c-" before the gene symbol of an oncogene indicates it is the cellular homolog (PROTO-ONCOGENES) of a v-oncogene.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

Established cell cultures that have the potential to propagate indefinitely.

The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.

Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.

The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.

A family of transforming proteins isolated from retroviruses such as MOUSE SARCOMA VIRUSES. They are viral-derived members of the raf-kinase family of serine-theonine kinases.

Transforming protein encoded by ras oncogenes. Point mutations in the cellular ras gene (c-ras) can also result in a mutant p21 protein that can transform mammalian cells. Oncogene protein p21(ras) has been directly implicated in human neoplasms, perhaps accounting for as much as 15-20% of all human tumors. This enzyme was formerly listed as EC 3.6.1.47.

Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.

An agency of the NATIONAL INSTITUTES OF HEALTH concerned with overall planning, promoting, and administering programs pertaining to advancement of medical and related sciences. Major activities of this institute include the collection, dissemination, and exchange of information important to the progress of medicine and health, research in medical informatics and support for medical library development.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

Research that involves the application of the natural sciences, especially biology and physiology, to medicine.

A ubiquitously expressed raf kinase subclass that plays an important role in SIGNAL TRANSDUCTION. The c-raf Kinases are MAP kinase kinase kinases that have specificity for MAP KINASE KINASE 1 and MAP KINASE KINASE 2.

Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.

Retroviral proteins that have the ability to transform cells. They can induce sarcomas, leukemias, lymphomas, and mammary carcinomas. Not all retroviral proteins are oncogenic.

An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus.

Normal cellular genes homologous to viral oncogenes. The products of proto-oncogenes are important regulators of biological processes and appear to be involved in the events that serve to maintain the ordered procession through the cell cycle. Proto-oncogenes have names of the form c-onc.

Cellular proteins encoded by the H-ras, K-ras and N-ras genes. The proteins have GTPase activity and are involved in signal transduction as monomeric GTP-binding proteins. Elevated levels of p21 c-ras have been associated with neoplasia. This enzyme was formerly listed as EC 3.6.1.47.

The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.

The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.

DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.

A strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) arising during the propagation of S37 mouse sarcoma, and causing lymphoid leukemia in mice. It also infects rats and newborn hamsters. It is apparently transmitted to embryos in utero and to newborns through mother's milk.

The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.

Species of GAMMARETROVIRUS, containing many well-defined strains, producing leukemia in mice. Disease is commonly induced by injecting filtrates of propagable tumors into newborn mice.

The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.

A replication-defective mouse sarcoma virus (SARCOMA VIRUSES, MURINE) first described by J.J. Harvey in 1964.

Small, monomeric GTP-binding proteins encoded by ras genes (GENES, RAS). The protooncogene-derived protein, PROTO-ONCOGENE PROTEIN P21(RAS), plays a role in normal cellular growth, differentiation and development. The oncogene-derived protein (ONCOGENE PROTEIN P21(RAS)) can play a role in aberrant cellular regulation during neoplastic cell transformation (CELL TRANSFORMATION, NEOPLASTIC). This enzyme was formerly listed as EC 3.6.1.47.

Protein kinases that catalyze the PHOSPHORYLATION of TYROSINE residues in proteins with ATP or other nucleotides as phosphate donors.

A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).

Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.

Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).

RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.

Proteins prepared by recombinant DNA technology.

Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.

Mitogenic peptide growth hormone carried in the alpha-granules of platelets. It is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication.

Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.

The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.

Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.

Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.

Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.

Federal, state, or local government organized methods of financial assistance.

A group of replication-defective viruses, in the genus GAMMARETROVIRUS, which are capable of transforming cells, but which replicate and produce tumors only in the presence of Murine leukemia viruses (LEUKEMIA VIRUS, MURINE).

Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.

Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.

Those individuals engaged in research.

Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.

The evaluation by experts of the quality and pertinence of research or research proposals of other experts in the same field. Peer review is used by editors in deciding which submissions warrant publication, by granting agencies to determine which proposals should be funded, and by academic institutions in tenure decisions.

Detailed financial plans for carrying out specific activities for a certain period of time. They include proposed income and expenditures.

A replication-defective murine sarcoma virus (SARCOMA VIRUSES, MURINE) capable of transforming mouse lymphoid cells and producing erythroid leukemia after superinfection with murine leukemia viruses (LEUKEMIA VIRUS, MURINE). It has also been found to transform cultured human fibroblasts, rat liver epithelial cells, and rat adrenocortical cells.

Retrovirus-associated DNA sequences (src) originally isolated from the Rous sarcoma virus (RSV). The proto-oncogene src (c-src) codes for a protein that is a member of the tyrosine kinase family and was the first proto-oncogene identified in the human genome. The human c-src gene is located at 20q12-13 on the long arm of chromosome 20.

Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.

A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors.

The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.

A CALMODULIN-dependent enzyme that catalyzes the phosphorylation of proteins. This enzyme is also sometimes dependent on CALCIUM. A wide range of proteins can act as acceptor, including VIMENTIN; SYNAPSINS; GLYCOGEN SYNTHASE; MYOSIN LIGHT CHAINS; and the MICROTUBULE-ASSOCIATED PROTEINS. (From Enzyme Nomenclature, 1992, p277)

Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.

DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.

A superfamily of PROTEIN-SERINE-THREONINE KINASES that are activated by diverse stimuli via protein kinase cascades. They are the final components of the cascades, activated by phosphorylation by MITOGEN-ACTIVATED PROTEIN KINASE KINASES, which in turn are activated by mitogen-activated protein kinase kinase kinases (MAP KINASE KINASE KINASES).

Experimentally induced new abnormal growth of TISSUES in animals to provide models for studying human neoplasms.

Proteins coded by oncogenes. They include proteins resulting from the fusion of an oncogene and another gene (ONCOGENE PROTEINS, FUSION).

The rate dynamics in chemical or physical systems.

A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.

Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.

Adherence of cells to surfaces or to other cells.

Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.

The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.

The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.

Transforming proteins coded by mos oncogenes. The v-mos proteins were originally isolated from the Moloney murine sarcoma virus (Mo-MSV).

CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)

Proteins that activate the GTPase of specific GTP-BINDING PROTEINS.

A serine-threonine protein kinase family whose members are components in protein kinase cascades activated by diverse stimuli. These MAPK kinases phosphorylate MITOGEN-ACTIVATED PROTEIN KINASES and are themselves phosphorylated by MAP KINASE KINASE KINASES. JNK kinases (also known as SAPK kinases) are a subfamily.

Retrovirus-associated DNA sequences (mos) originally isolated from the Moloney murine sarcoma virus (Mo-MSV). The proto-oncogene mos (c-mos) codes for a protein which is a member of the serine kinase family. There is no evidence as yet that human c-mos can become transformed or has a role in human cancer. However, in mice, activation can occur when the retrovirus-like intracisternal A-particle inserts itself near the c-mos sequence. The human c-mos gene is located at 8q22 on the long arm of chromosome 8.

An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.

A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.

A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.

Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.

Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)

Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.

CULTURE MEDIA free of serum proteins but including the minimal essential substances required for cell growth. This type of medium avoids the presence of extraneous substances that may affect cell proliferation or unwanted activation of cells.

Transport proteins that carry specific substances in the blood or across cell membranes.

A non-essential amino acid. In animals it is synthesized from PHENYLALANINE. It is also the precursor of EPINEPHRINE; THYROID HORMONES; and melanin.

A tyrosine-specific protein kinase encoded by the v-src oncogene of ROUS SARCOMA VIRUS. The transforming activity of pp60(v-src) depends on both the lack of a critical carboxy-terminal tyrosine phosphorylation site at position 527, and the attachment of pp60(v-src) to the plasma membrane which is accomplished by myristylation of its N-terminal glycine.

The parts of a macromolecule that directly participate in its specific combination with another molecule.

Protein factors that promote the exchange of GTP for GDP bound to GTP-BINDING PROTEINS.

Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-.

PROTEINS that specifically activate the GTP-phosphohydrolase activity of RAS PROTEINS.

Leukemia induced experimentally in animals by exposure to leukemogenic agents, such as VIRUSES; RADIATION; or by TRANSPLANTATION of leukemic tissues.

Specific receptors on cell membranes that react with PLATELET-DERIVED GROWTH FACTOR, its analogs, or antagonists. The alpha PDGF receptor (RECEPTOR, PLATELET-DERIVED GROWTH FACTOR ALPHA) and the beta PDGF receptor (RECEPTOR, PLATELET-DERIVED GROWTH FACTOR BETA) are the two principle types of PDGF receptors. Activation of the protein-tyrosine kinase activity of the receptors occurs by ligand-induced dimerization or heterodimerization of PDGF receptor types.

Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)

Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.

Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.

Substances that stimulate mitosis and lymphocyte transformation. They include not only substances associated with LECTINS, but also substances from streptococci (associated with streptolysin S) and from strains of alpha-toxin-producing staphylococci. (Stedman, 25th ed)

An oligopeptide produced by various bacteria which acts as a protease inhibitor.

Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.

A member of the Rho family of MONOMERIC GTP-BINDING PROTEINS. It is associated with a diverse array of cellular functions including cytoskeletal changes, filopodia formation and transport through the GOLGI APPARATUS. This enzyme was formerly listed as EC 3.6.1.47.

Elements of limited time intervals, contributing to particular results or situations.

Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.

Transforming proteins coded by sis oncogenes. Transformation of cells by v-sis is related to its interaction with the PDGF receptor and also its ability to alter other transcription factors.

A class of cellular receptors that have an intrinsic PROTEIN-TYROSINE KINASE activity.

Component of the NATIONAL INSTITUTES OF HEALTH. Through basic and clinical biomedical research and training, it conducts and supports research with the objective of cancer prevention, early stage identification and elimination. This Institute was established in 1937.

Matrine inhibits production and actions of fibrogenic cytokines released by mouse peritoneal macrophages. (1/5011)

AIM: To study the effects of matrine (Mat) on production and actions of fibrogenic cytokines from mouse peritoneal macrophages. METHODS: Mouse peritoneal macrophages were primed with calcimycin 1 micromol/L for 8 h then elicited by lipopolysaccharides (LPS) 100 microg/L for 6 h to induce fibrogenic cytokines. Proliferative and collagen stimulating activity in the macrophage culture supernatants was determined by crystal violet staining assay and [3H]-proline incorporation assay using rat hepatic stellate HSC-T6 cell or mouse fibroblast NIH3T3 cell. Transforming growth factor beta (TGFbeta) activity was measured by [3H]-thymidine incorporation assay using Mv-1-Lu mink lung epithelial cell. RESULTS: Mat (0.5-2 mmol/L) was shown to significantly inhibit LPS-induced collagen stimulating activities and TGFbeta production (P < 0.01) whereas did not inhibit proliferative activities induced by macrophages. Macrophage conditioned medium (MCM)-driven proliferation and collagen synthesis of HSC-T6 cells as well as NIH3T3 cells were attenuated by Mat (0.5-2 mmol/L) in a concentration-dependent manner. CONCLUSION: Antifibrotic effects of Mat on hepatic stellate cells may be related to reduction of fibrogenic cytokine production and blockade of their actions. (+info)

In vivo destabilization of dynamic microtubules by HDAC6-mediated deacetylation. (2/5011)

Trichostatin A (TSA) inhibits all histone deacetylases (HDACs) of both class I and II, whereas trapoxin (TPX) cannot inhibit HDAC6, a cytoplasmic member of class II HDACs. We took advantage of this differential sensitivity of HDAC6 to TSA and TPX to identify its substrates. Using this approach, alpha-tubulin was identified as an HDAC6 substrate. HDAC6 deacetylated alpha-tubulin both in vivo and in vitro. Our investigations suggest that HDAC6 controls the stability of a dynamic pool of microtubules. Indeed, we found that highly acetylated microtubules observed after TSA treatment exhibited delayed drug-induced depolymerization and that HDAC6 overexpression prompted their induced depolymerization. Depolymerized tubulin was rapidly deacetylated in vivo, whereas tubulin acetylation occurred only after polymerization. We therefore suggest that acetylation and deacetylation are coupled to the microtubule turnover and that HDAC6 plays a key regulatory role in the stability of the dynamic microtubules. (+info)

The formin-homology-domain-containing protein FHOD1 enhances cell migration. (3/5011)

Formin-homology-domain-containing proteins interact with Rho-family GTPases and regulate actin cytoskeleton organization and gene transcription. FHOD1 is a member of this family, interacts with Rac1 and induces transcription from the serum response element. In this study, we examined the effects of FHOD1 expression on cytoskeletal organization and function in mammalian cells. FHOD1 proteins were stably expressed in WM35 melanoma cells and NIH-3T3 fibroblasts. Cells expressing full-length FHOD1 demonstrated an elongated phenotype compared with vector-transfected cells and cells expressing a truncated FHOD1 (1-421) that lacks the conserved FH1 and FH2 domains. Full-length FHOD1 co-localized with filamentous actin at cell peripheries. Cells transiently expressing a C-terminal FHOD1 truncation mutant (DeltaC, residues 1-1010), which lacks an autoinhibitory protein-protein interaction domain, displayed prominent stress fibers. FHOD1 (1-421) did not induce stress fibers but localized to membrane ruffles in a manner similar to the full-length protein, indicating that the FH1 and FH2 domains are required for stress fiber appearance. FHOD1 DeltaC (1-1010)-dependent stress fibers were sensitive to dominant-negative RacN17 and the RhoA and ROCK inhibitors, C3 transferase and Y-27632. Stable overexpression of full-length FHOD1 enhanced the migration of WM35 and NIH-3T3 cells to type-I collagen and fibronectin, respectively. Cells expressing FHOD1 (1-421) migrated similar to control cells. Integrin expression and activation were not affected by FHOD1 expression. Moreover, FHOD1 overexpression did not alter integrin usage during adhesion or migration. These data demonstrate that FHOD1 interacts with and regulates the structure of the cytoskeleton and stimulates cell migration in an integrin-independent manner. (+info)

Dynamic association of RNA-editing enzymes with the nucleolus. (4/5011)

ADAR1 and ADAR2 are editing enzymes that deaminate adenosine to inosine in long double stranded RNA duplexes and specific pre-mRNA transcripts. Here, we show that full-length and N-terminally truncated forms of ADAR1 are simultaneously expressed in HeLa and COS7 cells owing to the usage of alternative starting methionines. Because the N-terminus of ADAR1 contains a nuclear export signal, the full-length protein localizes predominantly in the cytoplasm, whereas the N-terminally truncated forms are exclusively nuclear and accumulate in the nucleolus. ADAR2, which lacks a region homologous to the N-terminal domain of ADAR1, localizes exclusively to the nucleus and similarly accumulates in the nucleolus. Within the nucleolus, ADAR1 and ADAR2 co-localize in a novel compartment. Photobleaching experiments demonstrate that, in live cells, ADAR1 and ADAR2 are in constant flux in and out of the nucleolus. When cells express the editing-competent glutamate receptor GluR-B RNA, endogenous ADAR1 and ADAR2 de-localize from the nucleolus and accumulate at sites where the substrate transcripts accumulate. This suggests that ADAR1 and ADAR2 are constantly moving through the nucleolus and might be recruited onto specific editing substrates present elsewhere in the cell. (+info)

Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail. (5/5011)

Snail is a transcription repressor that plays a central role in the epithelium-mesenchyme transition (EMT), by which epithelial cells lose their polarity. Claudins and occludin are integral membrane proteins localized at tight junctions, which are responsible for establishing and maintaining epithelial cell polarity. We examined the relationship between Snail and the promoter activity of claudins and occludin. When Snail was overexpressed in cultured mouse epithelial cells, EMT was induced with concomitant repression of the expression of claudins and occludin not only at the protein but also at the mRNA level. We then isolated the promoters of genes encoding claudins and occludin, in which multiple E-boxes were identified. Transfection experiments with various promoter constructs as well as electrophoretic mobility assays revealed that Snail binds directly to the E-boxes of the promoters of claudin/occludin genes, resulting in complete repression of their promoter activity. Because the gene encoding E-cadherin was also reported to be repressed by Snail, we concluded that EMT was associated with the simultaneous repression of the genes encoding E-cadherin and claudins/occludin (i.e. the expression of adherens and tight junction adhesion molecules, respectively). (+info)

Antitumor effects of ZD6474, a small molecule vascular endothelial growth factor receptor tyrosine kinase inhibitor, with additional activity against epidermal growth factor receptor tyrosine kinase. (6/5011)

PURPOSE: Vascular endothelial growth factor (VEGF) is a major mitogen for endothelial cells and enhances vascular permeability. Enhanced VEGF secretion is found in human cancers and correlates with increased tumor neovascularization. ZD6474 is a p.o. bioavailable, VEGF flk-1/KDR receptor (VEGFR-2) tyrosine kinase inhibitor with antitumor activity in many human cancer xenografts and is currently in Phase I clinical development. EXPERIMENTAL DESIGN: We tested the effects of ZD6474 on EGFR phosphorylation in cell expressing functional epidermal growth factor receptor (EGFR) and the antiproliferative and the proapoptotic activity of ZD6474 alone or in combination taxanes in human cancer cell lines with functional EGFR but lacking VEGFR-2. The antitumor activity of this drug was also tested in nude mice bearing established GEO colon cancer xenografts. RESULTS: ZD6474 causes a dose-dependent inhibition of EGFR phosphorylation in mouse NIH-EGFR fibroblasts and human MCF-10A ras breast cancer cells, two cell lines that overexpress the human EGFR. ZD6474 treatment resulted in a dose-dependent inhibition of soft agar growth in seven human cell lines (breast, colon, gastric, and ovarian) with functional EGFR but lacking VEGFR-2. A dose-dependent supra-additive effect in growth inhibition and in apoptosis in vitro was observed by the combined treatment with ZD6474 and paclitaxel or docetaxel. ZD6474 treatment of nude mice bearing palpable GEO colon cancer xenografts (which are sensitive to inhibition of EGFR signaling) induced dose-dependent tumor growth inhibition. Immunohistochemical analysis revealed a significant dose-dependent reduction of neoangiogenesis. The antitumor activity of ZD6474 in GEO tumor xenografts was also found to be enhanced when combined with paclitaxel. Tumor regression was observed in all mice after treatment with ZD6474 plus paclitaxel, and it was accompanied by a significant potentiation in inhibition of angiogenesis. Six of 20 mice had no histological evidence of tumors after treatment with ZD6474 plus paclitaxel. CONCLUSIONS: This study suggests that in addition to inhibiting endothelial cell proliferation by blocking VEGF-induced signaling, ZD6474 may also be able to inhibit cancer cell growth by blocking EGFR autocrine signaling. These results provide also a rationale for the clinical evaluation of ZD6474 combined with taxanes in cancer patients. (+info)

Specific inhibition of the Akt1 pleckstrin homology domain by D-3-deoxy-phosphatidyl-myo-inositol analogues. (7/5011)

Activation of Akt (protein kinase B), a Ser/Thr protein kinase that promotes cell survival, has been linked to tumorigenesis. Akt is activated by phosphorylation after binding of its pleckstrin homology (PH) domain to plasma membrane phosphatidyl-myo-inositol-3-phosphates, formed by phosphoinositide-3-kinase. We report a novel strategy to inhibit Akt activation based on the use of D-3-deoxy-phosphatidyl-myo-inositols (DPIs) that cannot be phosphorylated on the 3-position of the myo-inositol ring. We have studied the DPIs, DPI 1-[(R)-2,3-bis(hexadecanoyloxy)propyl hydrogen phosphate], its ether lipid derivative DPI 1-[(R)-2-methoxy-3-octadecyloxypropyl hydrogen phosphate] (DPIEL), and its carbonate derivative DPI 1-[(R)-2-methoxy-3-octadecyloxypropyl carbonate]. We demonstrate in platelet-derived growth factor-stimulated mouse NIH3T3 cells that the DPIs bind to the PH domain of Akt, trapping it in the cytoplasm and thus preventing Akt activation. DPIEL did not inhibit myristylated-Akt, a constitutively active membrane-bound Akt expressed in NIH3T3 cells, and cell growth was not inhibited, unlike in wild-type NIH3T3 cells. Molecular modeling and docking studies show that DPIEL binds with much higher affinity to Akt's PH domain as compared with DPI and DPI 1-[(R)-2-methoxy-3-octadecyloxypropyl carbonate]. This study shows that the DPIs are a novel class of growth inhibitory agents with a novel mechanism of action through binding to the PH domain of Akt and inhibition of Akt activation. (+info)

Bfk: a novel weakly proapoptotic member of the Bcl-2 protein family with a BH3 and a BH2 region. (8/5011)

Proteins of the Bcl-2 family are critical regulators of apoptosis. Proapoptotic members, like Bax, contain three of the four Bcl-2 homology regions (BH1-3), while BH3-only proteins, like Bim, possess only the short BH3 motif. Database searches revealed Bfk, an unusual novel member of the Bcl-2 family that contains a BH2 and BH3 region but not BH1 or BH4. Bfk is thus most closely related to Bcl-G(L). It lacks a C-terminal membrane anchor and is cytosolic. Enforced expression of Bfk weakly promoted apoptosis and antagonized Bcl-2's prosurvival function. Like Bcl-G(L), Bfk did not bind to any Bcl-2 family members, even though its BH3 motif can mediate association with prosurvival proteins. Low amounts of Bfk were found in stomach, ovary, bone marrow and spleen, but its level in the mammary gland rose markedly during pregnancy, suggesting that Bfk may play a role in mammary development. (+info)

"NIH3T3 General Information". NIH 3T3 Cell Line. Retrieved 12 March 2021. Kristian Björnstad; Anders Helander; Peter Hultén; ... detects the viability of 3T3 cells after exposure to a chemical in the presence or absence of light. The 3T3 cell line was ... Cell culture can be an alternative to animal use in some cases. For example, cultured cells have been developed to create ... Tumoroids-3D cell cultures derived from cells biopsied from human patients-can be used in studying the genomics and drug ...

https://en.wikipedia.org/wiki/Alternatives_to_animal_testing

Effects on the Cytoskeleton in NIH/3T3 Cells". The Journal of Biochemistry. 125 (5): 960-965. doi:10.1093/oxfordjournals.jbchem ... growth factor of the cell such as to induce the formation of mitogen-activated protein interaction and playing a role in cell ...

https://en.wikipedia.org/wiki/Phoslactomycin_B

Expression of the mutant enzyme in NIH 3T3 cells". The Journal of Clinical Investigation. 86 (1): 351-5. doi:10.1172/JCI114708 ... Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell. 128 (1): 9-13. doi:10.1016/j.cell.2006.12.018. ... doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131. "Infection and Immunity Immunophenotyping (3i) Consortium". Tanoue ... July 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2 ...

https://en.wikipedia.org/wiki/PEPD

Enhanced motility in NIH 3T3 fibroblasts that overexpress gelsolin. Science. 1991; 251: 1233-1236. 113. Ohta, Y, Stossel, TP, ... Dev Cell, 2003, 4: 444-445. 77. Stossel, TP, Fenteany G, Hartwig, JH. Cell surface actin remodeling at a glance. J Cell Sci, ... The machinery of cell crawling. Sci Am. 1994; 271: 54-63. 69. Stossel, TP. The machinery of cell movements. The 1993 E. Donnall ... Cell crawling 20 years after Abercrombie, In, Cell Behavior: Control and Mechanism of Motility, JM Lackie, GA Dunn, GE Jones, ...

https://en.wikipedia.org/wiki/Thomas_P._Stossel

Cohen BD, Green JM, Foy L, Fell HP (Mar 1996). "HER4-mediated biological and biochemical properties in NIH 3T3 cells. Evidence ... Cell. 127 (1): 185-97. doi:10.1016/j.cell.2006.07.037. PMID 17018285. S2CID 18779079. "Entrez Gene: ERBB4 v-erb-a ... Culouscou JM, Plowman GD, Carlton GW, Green JM, Shoyab M (Sep 1993). "Characterization of a breast cancer cell differentiation ... Carpenter G (Mar 2003). "ErbB-4: mechanism of action and biology". Experimental Cell Research. 284 (1): 66-77. doi:10.1016/ ...

https://en.wikipedia.org/wiki/ERBB4

These cells developed into the commonly used cell line NIH 3T3. MEFs treated by mitomycin or gamma rays (such treatment makes ... Finally digest the remains by enzymes to obtain single isolated cells and culture the cells in a tissue culture dishes. MEF ... Cell. 126 (4): 663-676. doi:10.1016/j.cell.2006.07.024. hdl:2433/159777. PMID 16904174. (All articles with dead external links ... "Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines". J. Cell Biol ...

https://en.wikipedia.org/wiki/Mouse_embryonic_fibroblast

Kolch W, Heidecker G, Lloyd P, Rapp UR (January 1991). "Raf-1 protein kinase is required for growth of induced NIH/3T3 cells". ... Romero F, Martínez-A C, Camonis J, Rebollo A (June 1999). "Aiolos transcription factor controls cell death in T cells by ... cell migration, inhibition of apoptosis, and cell differentiation. Hereditary gain-of-function mutations of c-Raf are ... "X-linked and cellular IAPs modulate the stability of C-RAF kinase and cell motility". Nat. Cell Biol. 10 (12): 1447-55. doi: ...

https://en.wikipedia.org/wiki/C-Raf

"Sorting of tropomyosin isoforms in synchronised NIH 3T3 fibroblasts: evidence for distinct microfilament populations". Cell ... In the initial studies, transformation of rat embryo fibroblast cell line REF-52 and of normal rat kidney cells led to ... Nonmuscle tropomyosin isoforms function in all cells, both muscle and nonmuscle cells, and are involved in a range of cellular ... These studies have been extended to a number of cell types with similar results. Extensive studies in neuronal cells, ...

https://en.wikipedia.org/wiki/Tropomyosin

"Requirement for ras proto-oncogene function during serum-stimulated growth of NIH 3T3 cells". Nature. 313 (5999): 241-3. ... Cyclin D1 is expressed in all adult human tissues with the exception of cells derived from bone marrow stem cell lines (both ... Amplification of the CCND1 gene is present in: non-small cell lung cancers (30-46%) head and neck squamous cell carcinomas (30- ... Chromosomal translocation around the cyclin D1 gene locus is often seen in B mantle cell lymphoma. In mantle cell lymphoma, ...

https://en.wikipedia.org/wiki/Cyclin_D1

Ram, P. T.; Horvath, C. M.; Iyengar, R (2000). "Stat3-mediated transformation of NIH-3T3 cells by the constitutively active ... "Stat3-mediated transformation of NIH-3T3 cells by the constitutively active Q205L Galphao protein". Science. 287 (5450): 142-4 ... His laboratory focuses on how cell signals are routed and processed through cellular signaling networks within cells to ... Cell. 133 (4): 666-80. doi:10.1016/j.cell.2008.04.025. PMC 2728678. PMID 18485874. Bhalla, U. S.; Iyengar, R (1999). "Emergent ...

https://en.wikipedia.org/wiki/Ravi_Iyengar

"Human spectrin Src homology 3 domain binding protein 1 regulates macropinocytosis in NIH 3T3 cells". J. Cell Sci. 113 (21): ... Cell. 15 (1): 91-8. doi:10.1091/mbc.E03-06-0427. PMC 307530. PMID 14565974. Lanzetti L, Rybin V, Malabarba MG, Christoforidis S ... Cell. Biol. 20 (20): 7591-601. doi:10.1128/MCB.20.20.7591-7601.2000. PMC 86315. PMID 11003655. Xu J, Ziemnicka D, Merz GS, ... Cell Biol. 34 (10): 1178-83. doi:10.1016/S1357-2725(02)00064-X. PMID 12127568. Cardwell C (1977). "Student or worker? ...

https://en.wikipedia.org/wiki/ABI1

"Signaling through transforming G protein-coupled receptors in NIH 3T3 cells involves c-Raf activation. Evidence for a protein ... Ashkenazi A, Ramachandran J, Capon DJ (July 1989). "Acetylcholine analogue stimulates DNA synthesis in brain-derived cells via ... "Muscarinic m1 receptor-stimulated adenylate cyclase activity in Chinese hamster ovary cells is mediated by Gs alpha and is not ... when express in CHO cells, activates and downregulates both Gq alpha and G11 alpha equally and non-selectively". FEBS Letters. ...

https://en.wikipedia.org/wiki/Muscarinic_acetylcholine_receptor_M1

"The discovery of a 3-phosphomonoesterase that hydrolyzes phosphatidylinositol 3-phosphate in NIH 3T3 cells". J. Biol. Chem. 264 ...

https://en.wikipedia.org/wiki/Phosphatidylinositol-3-phosphatase

"Signaling through transforming G protein-coupled receptors in NIH 3T3 cells involves c-Raf activation. Evidence for a protein ... These receptors are highly expressed on pancreatic beta cells and are critical regulators of glucose homoestasis by modulating ... However, with respect to vasculature, activation of M3 on vascular endothelial cells causes increased synthesis of nitric oxide ... June 2006). "A critical role for beta cell M3 muscarinic acetylcholine receptors in regulating insulin release and blood ...

https://en.wikipedia.org/wiki/Muscarinic_acetylcholine_receptor_M3

"Signaling through transforming G protein-coupled receptors in NIH 3T3 cells involves c-Raf activation. Evidence for a protein ... "Identification and molecular characterization of a m5 muscarinic receptor in A2058 human melanoma cells. Coupling to inhibition ... receptor subtypes and neuronal nicotinic acetylcholine receptor subunits in human mononuclear leukocytes and leukemic cell ...

https://en.wikipedia.org/wiki/Muscarinic_acetylcholine_receptor_M5

Kochhar KS, Johnson ME, Volpert O, Iyer AP (1995). "Evidence for autocrine basis of transformation in NIH-3T3 cells transfected ... It is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, but also acts ... As well as the well-characterised effects of HGF on epithelial cells, endothelial cells and haemopoietic progenitor cells, HGF ... Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial ...

https://en.wikipedia.org/wiki/Hepatocyte_growth_factor

In one study, mutation of the SH3 domain disrupted SGEF-dependent functions in NIH-3T3 fibroblasts. In endothelial cells SGEF ... SGEF is also expressed in endothelial cells of the vasculature. Several widely used cell lines express this protein, these ... Cell. 15 (7): 3309-19. doi:10.1091/mbc.E04-02-0146. PMC 452585. PMID 15133129. Samson et al 2013 "The Guanine-Nucleotide ... SGEF was discovered during a screen for androgen-responsive genes in human prostate cancer cells. Subsequent northern blot ...

https://en.wikipedia.org/wiki/SGEF

Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to ... Cell Biol. 3 (4): 346-52. doi:10.1038/35070019. PMID 11283607. S2CID 36187702. Murányi A, Zhang R, Liu F, et al. (2001). " ... PPP1R12A Info with links in the Cell Migration Gateway Archived 2014-12-11 at the Wayback Machine v t e (Genes on human ... which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine ...

https://en.wikipedia.org/wiki/PPP1R12A

... is expressed at lower levels in NIH-3T3 fibroblasts, C2C12 myoblasts and Neuro-2A neuroblastoma cells. Dock11 mRNA has ... Côté JF, Vuori K (August 2007). "GEF what? Dock180 and related proteins help Rac to polarize cells in new ways". Trends in Cell ... Meller N, Merlot S, Guda C (November 2005). "CZH proteins: a new family of Rho-GEFs". Journal of Cell Science. 118 (Pt 21): ... Cell Cycle. 6 (6): 750-7. doi:10.4161/cc.6.6.3986. PMC 2040307. PMID 17361101. Lin Q, Yang W, Baird D, Feng Q, Cerione RA ( ...

https://en.wikipedia.org/wiki/Dock11

Type 3 protein kinase C localization to the nuclear envelope of phorbol ester-treated NIH 3T3 cells. J. Cell. Biol., 109:685- ... "Type 3 protein kinase C localization to the nuclear envelope of phorbol ester-treated NIH 3T3 cells". The Journal of Cell ... Molecular Cell 26: 393-402, 2007. Nagiec, Eva E.; Wu, Luping; Swaney, Steve M.; Chosay, John G.; Ross, Daniel E.; Brieland, ... By showing mitochondrial protein synthesis was the link to Zyvox cytotoxicity in human cells, this research led to important ...

https://en.wikipedia.org/wiki/Karen_Leach

Chen M, She H, Kim A, Woodley DT, Li W (November 2000). "Nckbeta adapter regulates actin polymerization in NIH 3T3 fibroblasts ... In vitro studies using cultured cells indicate that endothelial cells secrete PDGF, which recruits PDGFRβ-expressing pericytes ... Olson LE, Soriano P (2011). "PDGFRβ signaling regulates mural cell plasticity and inhibits fat development". Developmental Cell ... Cell. Biol. 12 (12): 5824-33. doi:10.1128/MCB.12.12.5824. PMC 360522. PMID 1333047. Chen M, She H, Davis EM, Spicer CM, Kim L, ...

https://en.wikipedia.org/wiki/PDGFRB

"Activation of mitogen-activated protein kinase kinase by v-Raf in NIH 3T3 cells and in vitro". Science. 257 (5075): 1404-7. ... Cell. Biol. 16 (3): 1094-102. doi:10.1128/mcb.16.3.1094. PMC 231092. PMID 8622654. Zinck R, Hipskind RA, Pingoud V, Nordheim A ... Cell. Biol. 13 (8): 4539-48. doi:10.1128/mcb.13.8.4539. PMC 360070. PMID 8393135. Moodie SA, Willumsen BM, Weber MJ, Wolfman A ... Given its role as a transcription factor, Elk1 is expressed in the nuclei of non-neuronal cells. The protein is present in the ...

https://en.wikipedia.org/wiki/ELK1

Expression of a splice variant of the receptor for GHRH in 3T3 fibroblasts activates cell proliferation responses to GHRH ... www.ncbi.nlm.nih.gov/pmc/articles/PMC27064/. *Salvatori R, Fan X, Phillips JA, et al. (2001). „Three new mutations in the gene ... www.ncbi.nlm.nih.gov/pmc/articles/PMC117538/. *Salvatori R, Fan X, Mullis PE, et al. (2002). „Decreased expression of the GHRH ... Gaylinn BD (1999). „Molecular and cell biology of the growth hormone-releasing hormone receptor.". Growth Horm. IGF Res. 9 ...

https://sh.wikipedia.org/wiki/Hormon_rasta_oslobađajući_hormonski_receptor

"Increased growth of NIH/3T3 cells by transfection with human p120 complementary DNA and inhibition by a p120 antisense ... Overexpression of p120 leads to malignant transformation of 3T3 cells while treatment with antisense p120 mRNA causes the ... "A region of antisense RNA from human p120 cDNA with high homology to mouse p120 cDNA inhibits NIH 3T3 proliferation". Cancer ... Cell. 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. v t e (Genes on human chromosome 12, All ...

https://en.wikipedia.org/wiki/NOL1

... factor-1 and the heat shock cognate 70 protein associate in high molecular weight complexes in the cytoplasm of NIH-3T3 cells ... Cell. 122 (6): 957-68. doi:10.1016/j.cell.2005.08.029. hdl:11858/00-001M-0000-0010-8592-0. PMID 16169070. S2CID 8235923. Imai Y ... an apoptotic cell undergoes structural changes including cell shrinkage, plasma membrane blebbing, nuclear condensation, and ... "Intracellular localization and partial amino acid sequence of a stress-inducible 40-kDa protein in HeLa cells". Cell Structure ...

https://en.wikipedia.org/wiki/HSPA8

DNA from a rhabdomyosarcoma cell line and a fibrosarcoma cell line transformed a NIH/3T3 mouse fibroblast cell line. After ... He first realised that the addition of bovine fetal calf serum (FCS) to Swiss 3T3 cells increased the polymerisation of actin ... "Prominent Cell Biologist Dies". In Memoriam: Alan Hall, Pioneer in Rho GTPases and Chair of Cell Biology at Sloan-Kettering "At ... cells. Downregulation of RhoA in the HBE cell lines using siRNAs showed a lack of apical junction formation in contrast with ...

https://en.wikipedia.org/wiki/Alan_Hall

These cells are similar to human primary preadipocytes, and may or may not become a popular model instead of Mouse 3T3-L1 cells ... Simpson-Golabi-Behmel syndrome at NIH's Office of Rare Diseases GeneReview/NCBI/NIH/UW entry on Simpson-Golabi-Behmel Syndrome ... It has been suggested that SGBS type II may be caused by duplication of the GPC4 gene, which helps to regulate cell division ... The function of this gene is to produce a protein that acts as a cell surface receptor that binds to transcription factors. ...

https://en.wikipedia.org/wiki/Simpson–Golabi–Behmel_syndrome

"Gene expression analysis suggests that EBF-1 and PPARgamma2 induce adipogenesis of NIH-3T3 cells with similar efficiency and ... EBF1 stands for Early B-Cell Factor 1. EBF1 controls the expression of key proteins required for B cell differentiation, signal ... "Early B-cell factor 1 (EBF1) is critical for transcriptional control of SLAMF1 gene in human B cells". Biochimica et Biophysica ... "Early B Cell Factor 1 Regulates B Cell Gene Networks by Activation, Repression, and Transcription- Independent Poising of ...

https://en.wikipedia.org/wiki/EBF1

... and downmodulation of CSF-1 receptors in NIH 3T3 cells transformed by cotransfection of the human CSF-1 and c-fms (CSF-1 ... and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By ... is a secreted cytokine which causes hematopoietic stem cells to differentiate into macrophages or other related cell types. ... Eukaryotic cells also produce M-CSF in order to combat intercellular viral infection. It is one of the three experimentally ...

https://en.wikipedia.org/wiki/Macrophage_colony-stimulating_factor

doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. v t e (Genes on human chromosome 11, All stub articles, Human ... 2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome ... "Role of EHD1 and EHBP1 in perinuclear sorting and insulin-regulated GLUT4 recycling in 3T3-L1 adipocytes". J. Biol. Chem. 279 ( ... Cell. 15 (5): 2410-22. doi:10.1091/mbc.E03-10-0733. PMC 404033. PMID 15020713. Guilherme A, Soriano NA, Furcinitti PS, Czech MP ...

https://en.wikipedia.org/wiki/EHD1

It also promotes the transition through G1 during the cell cycle and causes tumorgenic transformation of NIH/3T3 fibroblasts. ... enabling the cells to migrate. FGD1 also activates the c-Jun N-terminal kinase (JNK) signaling cascade, important in cell ... a key player in the establishment of cell polarity in all eukaryotic cells. The GEF activity of FGD1, which activates Cdc42, is ... Cell. 20 (9): 2413-27. doi:10.1091/mbc.E08-11-1136. PMC 2675621. PMID 19261807. Olson MF, Pasteris NG, Gorski JL, Hall A ( ...

https://en.wikipedia.org/wiki/FGD1

... l cells MeSH A11.329.228.900 - 3t3 cells MeSH A11.329.228.900.080 - balb 3t3 cells MeSH A11.329.228.900.550 - nih 3t3 cells ... llc-pk1 cells MeSH A11.251.210.700 - 3t3 cells MeSH A11.251.210.700.080 - balb 3t3 cells MeSH A11.251.210.700.550 - nih 3t3 ... cells MeSH A11.251.210.700.775 - Swiss 3t3 cells MeSH A11.251.210.700.775.800 - 3t3-l1 cells MeSH A11.251.210.955 - vero cells ... Swiss 3t3 cells MeSH A11.329.228.900.775.800 - 3t3-l1 cells MeSH A11.329.228.950 - mesangial cells MeSH A11.329.372.300 - ...

https://en.wikipedia.org/wiki/List_of_MeSH_codes_(A11)

Studies on cultured immortalized human dermal keratinocyte (i.e. HDK) and mouse embryonic fibroblast NIH-3T3 cell lines found ... that the YAP1-NUTM1 and WWTR1-NUTM1 fusion genes stimulated the anchorage-independent growth of NIH-3T3 cells and activated a ... Overexpression of the MYC and SOX2 genes can also act to maintain cells in an undifferentiated stem cell-like state similar to ... round cell, and epithelioid cell-like features which are regarded as sarcomas. Sarcomas with NUTM1 fusion genes typically a) ...

https://en.wikipedia.org/wiki/Nuclear_protein_in_testis_gene

Two alternative splicing products of mouse Naa10, mNaa10235 and mNaa10225, were reported in NIH-3T3 and JB6 cells that may have ... including cell migration, cell cycle control, DNA damage control, caspase-dependent cell death, p53 dependent apoptosis, cell ... Cell. 146 (4): 607-20. doi:10.1016/j.cell.2011.06.050. PMC 3182480. PMID 21854985. Kuo HP, Lee DF, Chen CT, Liu M, Chou CK, Lee ... Whiteway M, Szostak JW (December 1985). "The ARD1 gene of yeast functions in the switch between the mitotic cell cycle and ...

https://en.wikipedia.org/wiki/N-alpha-acetyltransferase_10

The novel catenin p120cas binds classical cadherins and induces an unusual morphological phenotype in NIH 3T3 fibroblasts. ( ... Kaiso depletion attenuates the growth and survival of triple negative breast cancer cells. (2017) Cell Death and Disease 8, ... Kaiso differentially regulates components of the Notch signaling pathway in intestinal cells. (2017) Cell Communication and ... promotes cell migration and invasion of prostate cancer cells through regulation of miR-31 expression. (2016) Oncotarget 7(5): ...

https://en.wikipedia.org/wiki/Juliet_Daniel

A similar in vitro decrease in proliferation occurred in NIH 3T3 cells and a human melanoma cell line expressing antisense ... RT101 cells with perlecan knocked down by antisense did not show tumor formation in this system, however cells expressing the ... MeWo cells are characteristically less invasive than their clonal variant cell line 70W. One lab studied perlecan expression in ... This was first shown in colon cancer cell lines, and subsequently in cell lines of other tissue origins, but in each case ...

https://en.wikipedia.org/wiki/Perlecan

Aoyama A, Fröhli E, Schäfer R, Klemenz R (Mar 1993). "Alpha B-crystallin expression in mouse NIH 3T3 fibroblasts: ... "Moonlighting cell-surface GAPDH recruits apotransferrin to effect iron egress from mammalian cells" (PDF). Journal of Cell ... In wild type cells, this enzyme is present as enzymatically active AO octamers in the peroxisomal matrix. However, in cells ... The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a ...

https://en.wikipedia.org/wiki/Protein_moonlighting

Polybrene in combination with DMSO shock is used to transfect some cell types such as NIH-3T3 and CHO. It has other uses, ... Currently, it is primarily used to increase the efficiency of transduction of certain cells with retrovirus in cell culture. ... Hexadimethrine bromide acts by neutralizing the charge repulsion between virions and sialic acid on the cell surface. Use of ... "Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent virus adsorption on the target cell ...

https://en.wikipedia.org/wiki/Hexadimethrine_bromide

The expression of calponin 2 in NIH/3T3 cells was decreased when cytoskeleton tension was reduced after blebbstatin inhibition ... lung alveolar cells, endothelial cells, myeloid white blood cells, platelet, B lymphocyte, and myoblasts. These cell types can ... epithelial and endothelial cells), b) cells that have high rates of proliferation (e.g., myoblasts), and c) cells that are ... "Diminished expression of h2-calponin in prostate cancer cells promotes cell proliferation, migration and the dependence of cell ...

https://en.wikipedia.org/wiki/Calponin_2

Mitogen-activated protein kinase activation resulting from selective oncogene expression in NIH 3T3 and Rat 1a cells. Proc Natl ... In his work with stem cells, they defined a mutation that captured self-renewing tissue stem cell in a permanent state of EMT. ... 20: 2516-2522 PMC4024346 My Bibliography - NCBI (nih.gov) "NIGMS MERIT Awards". www.nigms.nih.gov. Retrieved 2020-12-01. Gary L ... Mol Cell Biol 33, 3011-3025. PMC3719671 Johnson, G.L., Stuhlmiller, T.J., Angus, S.P., Zawistowski, J.S., Graves, L.M. (2014) ...

https://en.wikipedia.org/wiki/Gary_L._Johnson

... evidence that its overexpression is sufficient to transform NIH/3T3 cells". The EMBO Journal. 7 (8): 2465-73. doi:10.1002/j. ... Cytogenetics and Cell Genetics. 60 (2): 114-6. doi:10.1159/000133316. PMID 1611909. "Entrez Gene: MCF2 MCF.2 cell line derived ... The commonly-used name DBL is derived from "diffuse B-cell lymphoma", the cancer type where this gene was first identified as ... April 1991). "A region of proto-dbl essential for its transforming activity shows sequence similarity to a yeast cell cycle ...

https://en.wikipedia.org/wiki/MCF2

"Purified plasma membranes inhibit polypeptide growth factor-induced DNA synthesis in subconfluent 3T3 cells". Journal of Cell ... He then spent one year as an NIH staff scientist in Tom Reese's laboratory at the Marine Biological Laboratory at Woods Hole, ... Cell. 126 (2): 335-348. doi:10.1016/j.cell.2006.05.046. PMC 2851639. PMID 16873064. Carter, Andrew P.; Garbarino, Joan E.; ... He was the president of the American Society for Cell Biology in 2012. He has also been an investigator at the Howard Hughes ...

https://en.wikipedia.org/wiki/Ronald_Vale

... to do this they did the study on NIH/3T3 cells and in the lung tissue of rats. They found that the fumes that the NIH/3T3 cells ... 1α through PI3K/ERK pathway in NIH/3T3 cells". Journal of Occupational Health. 58 (5): 413-424. doi:10.1539/joh.15-0287-OA. ... that high exposure to Ni-smelting fumes may be harmful as Ni-smelting fumes may be a potential carcinogen in mammalian cells. ...

https://en.wikipedia.org/wiki/Nickel_mine

SH3 domain-binding site in SLP-76 required for T-cell receptor-mediated activation of PLC-gamma1 and NFAT". Mol. Cell. Biol. 21 ... GeneReviews/NIH/NCBI/UW entry on Myofibrillar Myopathy Human BAG3 genome location and BAG3 gene details page in the UCSC Genome ... "Potential role of Gab1 and phospholipase C-gamma in osmotic shock-induced glucose uptake in 3T3-L1 adipocytes". Horm. Metab. ... linker for activation of T cells) required for recruitment and activation of signalling proteins in T cells". Biochem. J. 356 ( ...

https://en.wikipedia.org/wiki/BAG3

"Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells". Genes Dev. 5 (9): 1538-52. doi:10.1101 ... GeneReviews/NIH/NCBI/UW entry on Familial Acute Myeloid Leukemia (AML) with Mutated CEBPA CEBPA+protein,+human at the US ... Cell. 8 (4): 817-28. doi:10.1016/S1097-2765(01)00366-5. PMID 11684017. Ho PA, Alonzo TA, Gerbing RB, Pollard J, Stirewalt DL, ... The encoded protein is a key regulator of adipogenesis (the process of forming new fat cells) and the accumulation of lipids in ...

https://en.wikipedia.org/wiki/CEBPA

Kochhar KS, Johnson ME, Volpert O, Iyer AP (1995). "Evidence for autocrine basis of transformation in NIH-3T3 cells transfected ... Normally, only stem cells and progenitor cells express MET, which allows these cells to grow invasively in order to generate ... phagocytes and NK cells), thus forming a bridge between an effector and a target cells. This induces the effector cell ... However, cancer stem cells are thought to hijack the ability of normal stem cells to express MET, and thus become the cause of ...

https://en.wikipedia.org/wiki/C-Met

"Cineromycin B isolated from Streptomyces cinerochromogenes inhibits adipocyte differentiation of 3T3-L1 cells via Krüppel-like ... "Cineromycin B". pubchem.ncbi.nlm.nih.gov. Matsuo, Hirotaka; Kondo, Yoshiyuki; Kawasaki, Takashi; Imamura, Nobutaka (August 2015 ...

https://en.wikipedia.org/wiki/Cineromycin_B

The phenotypes of NIH 3T3 cells transfected with basic fibroblast growth factor (bFGF) cDNAs that express only the high ... Selective expression of high molecular weight basic fibroblast growth factor confers a unique phenotype to NIH 3T3 cells. In: ... Selective expression of high molecular weight basic fibroblast growth factor confers a unique phenotype to NIH 3T3 cells. ... Selective expression of high molecular weight basic fibroblast growth factor confers a unique phenotype to NIH 3T3 cells. / ...

https://moh-it.pure.elsevier.com/en/publications/selective-expression-of-high-molecular-weight-basic-fibroblast-gr-2

This is part of a triplicate data set of non-overlapping fields of NIH 3T3 fibroblasts cultured on polystyrene. Each set is ... Cell Type. permanent cell line cell Cell Line. NIH/3T3 Cellular Component. cell nucleus extracellular matrix part ... This is part of a triplicate data set of non-overlapping fields of NIH 3T3 fibroblasts cultured on polystyrene. Each set is ... Protocol: The cells were seeded on TCPS dishes at ~1000 cells/well during a passage cycle. The test cultures were incubated ...

http://www.cellimagelibrary.org/images/7829

We discovered that exposure of cell or tissue lysates to a biotinylated isoxazole (b-iso … ... Eukaryotic cells contain assemblies of RNAs and proteins termed RNA granules. Many proteins within these bodies contain KH or ... NIH 3T3 Cells * RNA / metabolism* * RNA-Binding Proteins / analysis* * RNA-Binding Proteins / chemistry ... DOI: 10.1016/j.cell.2012.04.017 Abstract Eukaryotic cells contain assemblies of RNAs and proteins termed RNA granules. Many ...

https://pubmed.ncbi.nlm.nih.gov/22579281/?dopt=Abstract

NIH-3T3 cells exposed to normal mouse brain and passaged 30 times (lane 3); NIH-3T3... ... Finally, we used NIH-3T3 (ATCC-CRL1658) and L929 (ATCC-CCL1), both mouse embryo fibroblast cells, known to be infectable with ... The same protocol was used with NIH-3T3 and L929 murine cells exposed to mouse-adapted 22L scrapie agent (as positive controls ... We exposed monolayers of NIH-3T3 and L929 murine fibroblast cells to the mouse-adapted 22L strain of scrapie agent and observed ...

https://wwwnc.cdc.gov/eid/article/17/12/11-0607_article

Cell spreading and cell expression depended on the porosity and pore-size. ... The attachment of human articular cartilage cells were investigated on these bioprotein matrices. The chondrocytes produced an ... Interactions between Spider Silk and Cells - NIH/3T3 Fibroblasts Seeded on Miniature Weaving Frames. *J. W. Kuhbier, C. ... Its non-homogeneous surface can explain the existence of cells spreading in specific directions and the presence of cell ...

https://www.semanticscholar.org/paper/Silkworm-and-spider-silk-scaffolds-for-chondrocyte-Gellynck-Verdonk/9efd3625bcd4dc3ebe088b0b53929ff09c00b034

NIH 3T3 Cells. 1. 2013. 701. 0.030. Why? Protein Interaction Domains and Motifs. 1. 2014. 522. 0.030. Why? ... Funded by the NIH National Center for Advancing Translational Sciences through its Clinical and Translational Science Awards ...

https://connects.catalyst.harvard.edu/Profiles/display/Person/154084/Network/ResearchAreas/details

http://www.cellimagelibrary.org/images/7909

http://cellimagelibrary.org/images/9004

Cytotoxicity was performed against Artemia salina and mouse embryonic fibroblast NIH/3T3 cell line following contact toxicity ... In MTT cells viability assay, Ph.Chf, Ph.EtAc, Ph.Sp were most active causing 79.00, 72.50, and 71.50% cytotoxicity, respectiv ... In MTT cells viability assay, Ph.Chf, Ph.EtAc, Ph.Sp were most active causing 79.00, 72.50 and 71.50% cytotoxicity respectively ... and MTT cells viability assays, respectively. The GC-MS analysis of Ph.Cr, Ph.Hex, Ph.Chf, Ph.Bt, and Ph.EtAc identified 126, ...

https://www.frontiersin.org/articles/10.3389/fphar.2016.00074/full

Visualization of Intracellular Hydrogen Peroxide with the Genetically Encoded Fluorescent Probe HyPer in NIH-3T3 Cells. Methods ... Photolysis of cell-permeant caged inositol pyrophosphates controls oscillations of cytosolic calcium in a β-cell line. Chem Sci ... Regulation of Calcium Oscillations in β-Cells by Co-activated Cannabinoid Receptors. Cell Chem Biol. 2020 Nov 2:S2451-9456(20) ... "MiniVIPER is a peptide tag for imaging and translocating proteins in cells." Biochemistry 59(33), 3051-3059 (2020). ...

https://www.ohsu.edu/school-of-medicine/chemical-physiology-and-biochemistry/publications

MCMV was grown and purified on NIH 3T3 cells. Briefly, NIH 3T3 cells were seeded in 15-cm dishes in complete DMEM and were ... C and viral titers were measured by plaque assay in NIH 3T3 cells. Ten-fold serial dilutions of MCMV were added to NIH 3T3 ... NIH 3T3 and MLE12 cell lines were cultured in DMEM and DMEM/F12, supplemented with 10% fetal bovine serum (FBS) (Gibco, USA), 1 ... MLE-12 cells were seeded into confocal culture dishes at a density of 1 × 105 cells per well. When the cells grow to about 80% ...

https://www.researchsquare.com/article/rs-1046275/v1

C6 and NIH/3T3 cells, using PAK1 Antibody.. Western blot analysis of extracts from HeLa cells transfected with 100 nM control ... Western blot analysis of extracts from SH-SY5Y, C6 and NIH/3T3 cells, using PAK1 Antibody.. Show Less Show More ... 10X Cell Lysis Buffer: (#9803) To prepare 10 ml of 1X cell lysis buffer, add 1 ml cell lysis buffer to 9 ml dH2O, mix. ... Cell Lysate Pre-Clearing (Optional). *Vortex to mix beads.. *Add 10-30 µl of 50% Protein A agarose bead slurry to 200 µl cell ...

https://www.cellsignal.com/products/primary-antibodies/pak1-antibody/2602?N=102236+4294960382+4294956287&fromPage=plp

Invasiveness and metastasis of NIH 3T3 cells induced by Met-hepatocyte growth factor/scatter factor autocrine stimulation. Proc ... Cell Culture. Flasks containing cells were stored in cell incubators maintained at 37°C and a 5% CO2 concentration. Cell lines ... Western Blot Analysis of Cell Lines for MET Expression. For immunoblot assays, cells were harvested and cell pellets were ... C) Western blot results from MET/HGF-producing U87MG cells, MET-producing MKN45 cells, SKOV3 ovarian cancer cells for reference ...

https://jnm.snmjournals.org/content/58/9/1386

Reorganization of actomyosin is an essential process for cell migration and myosin regulatory light chain (MLC20) ... B) Immunodepletion of ZIP kinase from COS 7 and NHI3T3 cell extracts. Cell extracts from COS 7 and NIH3T3 cells were subjected ... Calyculin-A increases the level of protein phosphorylation and changes the shape of 3T3 fibroblasts. Cell Motil. Cytoskeleton. ... B) Immunodepletion of ZIP kinase from COS 7 and NHI3T3 cell extracts. Cell extracts from COS 7 and NIH3T3 cells were subjected ...

https://rupress.org/jcb/article/165/2/243/33868/ZIP-kinase-is-responsible-for-the-phosphorylation

NIH/3T3 cell line stained with HM34 biotin, followed by Sav-PE * NIH/3T3 cell line stained with HM34 biotin, followed by Sav-PE ... Cell Type Endothelial cells, Hematopoietic stem and progenitors Biology Area Cell Biology, Immunology, Neuroinflammation, ... CD34 is thought to function as an adhesion molecule for attachment of stem cells to extracellular matrix or stromal cells. ... Mouse CD34 transfected BHK cells Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Preparation ...

https://www.biolegend.com/fr-fr/products/biotin-anti-mouse-cd34-antibody-5069

erbB-2 is a potent oncogene when overexpressed in NIH/3T3 cells. Science 1987;237:178-82. [PUBMED] [FULLTEXT] ... Mol Cell Biol 1986;6:955-8. 4.. Ullrich A, Schlesinger J. Signal transduction by receptors with tyrosine kinase activity. Cell ... of the tumor cells is negative (Score 1). No staining or staining in less than 10% of the cells is negative (Score 0). ... Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore - 12 India. Source of Support: KV is ...

https://www.indianjcancer.com/article.asp?issn=0019-509X

... expressed in HEK 293 cells; Synonyms: EC: 2.7.10.1,Epidermal growth factor receptor,Proto-oncogene c-ErbB-1,Receptor tyrosine- ... Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected EGFR in HeLa, A431, HUVEC, and NIH 3T3 cells. ... Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected EGFR in A431 cell lysate.. ... 250 dilution from a representative lot detected EGFR lysates from wild type and EGFR knockout HeLa cells. ...

https://www.sigmaaldrich.com/HK/en/product/sigma/zrb04338

... significantly inhibited LPS-induced inflammatory responses in RAW264.7 cells and that anti-inflammatory activity of coffee ... coffee extract did not affect the TNFα-induced activation of NF-κB in NIH-3T3 cells stably expressing the NF-κB-dependent ... Cell culture and the treatment with LPS. The murine macrophage cell line, RAW264.7 was purchased from the Riken Cell Bank ( ... Measurement of cell viability. RAW264.7 cells (5 × 105 cells) were seeded in a 24-well plate and preincubated with coffee ...

https://www.nature.com/articles/s41598-020-59380-x?error=cookies_not_supported&code=970d1222-7b9c-4f10-a5de-fa36a01afc50

Finally, agonist efficacy was assessed by measurement of phosphoinositide hydrolysis in NIH 3T3 cells expressing the rat 5-HT2A ... In addition, these compounds were evaluated for their ability to compete for agonist and antagonist binding to cells expressing ...

https://erowid.org/references/refs_view.php?ID=6485&S=Monte_AP&SField=Author

In this in vitro burn model, using the cell line NIH-3T3 was observed drastic cellular injury with significant changes in cell ... METHODS: Induction of thermal injury in cultures of mouse fibroblast - NIH-3T3- cell line and determination of cell viability ... CONCLUSION: The burn model in vitro using the cell line NIH-3T3 was reproductive and efficient. This burn model was possible to ... PURPOSE: To propose an experimental burn model in NIH-3T3 cell line. ...

https://www.scielo.br/j/acb/i/2014.v29suppl2/

2 can be achieved by transiently transfecting cells in culture with standard transfection protocols ... pACP-ADRβ2 has performed well in transient transfection of CHO-K1, COS-7, U-2 0S and NIH 3T3 cells. In most cases, ACP-ADRβ2 ... It is possible to use pools of stable cell populations for initial cell labeling to test for the presence of ACP-tag expression ... depending on the cell line. It is recommended that a kill curve be established for each cell line to determine optimal ...

https://www.neb.com/protocols/0001/01/01/expression-of-acp-adr2-fusions-n9321

... converts the ST5 p70 protein from an inhibitor of RAS signaling to an activator with transforming activity in NIH-3T3 cells. ... Cell Growth Differ, 1992 Aug. PMID 1390339 * DENND2B activates Rab13 at the leading edge of migrating cells and promotes ... Expression of an isoform of the novel signal transduction protein ST5 is linked to cell morphology. Hubbs AE, et al. Oncogene, ... This gene was identified by its ability to suppress the tumorigenicity of Hela cells in nude mice. The protein encoded by this ...

https://www.ncbi.nlm.nih.gov/gene/6764

Primary CECs and mouse NIH 3T3 cells treated with the two FGF1 derivatives showed equivalent EC50 ranges (3.3-24 vs.1.9-16. ng/ ... This chapter gives an overview on cell-cell and cell-matrix interactions. Cell signaling pathways form extensive interactive ... Here the proteome of the brain metastatic breast cancer cell line 231-BR has been compared with that of the parental cell line ... Human Embryonic Kidney Cells (HEK-293 Cells): Characterization and Dose-Response Relationship for Modulated Release of Nerve ...

https://www.researchgate.net/profile/Ralph-Bradshaw-2

HeLa human carcinoma cells (ATCC CCL-2), Jurkat T leukemia cells (ATCC TIB-152) and NIH 3T3 mouse fibroblast cells (ATCC CRL- ... Cells were seeded in a 96 well imaging plate (Cat. No. 353219) at ~10,000 cells per well. After overnight incubation, cells ... Cells were seeded in a 96 well imaging plate (Cat. No. 353219) at ~10,000 cells per well. After overnight incubation, cells ... Cells were seeded in a 96 well imaging plate (Cat. No. 353219) at ~10,000 cells per well. After overnight incubation, cells ...

https://www.bdbiosciences.com/en-be/products/reagents/microscopy-imaging-reagents/immunofluorescence-reagents/purified-mouse-anti-cytochrome-c.556432

Thesis: Activation-Induced Changes in the Histamine Content of Murine Mast Cells and their Adhesion to NIH-3T3 Fibroblasts: PhD ... Thesis: STK38L Kinase Ablation Promotes Loss of Cell Viability in a Subset of KRAS-Dependent Pancreatic Cancer Cell Lines: PhD ... Thesis: Sustained CA2+ mobilizations: a quantitative approach to predict their importance in cell-cell communication: PhD in ... Thesis: The role of PKC-theta in IL 12-induced IFN-gamma production by natural killer cells: PhD in Pharmacology. Advisor: ...

https://www.bumc.bu.edu/busm-pm/people/alumni/

CD140a-transfected NIH 3T3 cells. Concentration 1 mg/ml. Preparation Purified by protein-A affinity chromatography. ... Mol Cell Biol. 2008 Sep;28(18):5646-57. PubMed Andrae J, Gallini R, Betsholtz C: Role of platelet-derived growth factors in ... Separation of cells stained using anti-human CD140a (16A1) purified antibody (concentration in sample 0,33 μg/ml, GAM APC, red- ... CD140a / PDGF-RA (platelet-derived growth factor receptor alpha) is a cell surface receptor for members of platelet-derived ...

https://www.exbio.cz/research-product/antibodies/cd-and-related-antigens/anti-hu-cd140a-purified

D2.948.681.202.550 NIH 3T3 Cells A11.251.210.700.550 A11.251.210.100.550 A11.329.228.900.550 A11.329.228.100.550 Nucleic Acid ... D8.811.682.47.820.193 3T3 Cells A11.251.210.700 A11.251.210.100 A11.329.228.900 A11.329.228.100 3T3-L1 Cells A11.251.210.700. ... A15.145.229.637.555.567.562.200.300 BALB 3T3 Cells A11.251.210.700.80 A11.251.210.100.80 A11.329.228.900.80 A11.329.228.100.80 ... G16.100.57.500.535.693 Swiss 3T3 Cells A11.251.210.700.775 A11.251.210.100.775 A11.329.228.900.775 A11.329.228.100.775 ...

https://decs.bvs.br/P/mnchg2014.txt

T1 cells had been grown up in Y-10 moderate, and COS-1 and NIH 3T3 cells early grown up in DMEM/Y-12 (1:1 mix) supplemented ... NIH 3T3 and CHO-K1 cells had been treated with 10 g/ml Taxol (Sigma-Aldrich), 20 Meters forskolin, and 100 nM or 1 Meters OA ... Cells had been transfected by FuGENE 6 (Applied Research). Medication 207679-81-0 treatment was utilized in some trials before ... COS-1 cells had been incubated with 1 Meters okadaic acidity (OA) or 50 Meters staurosporine (Calbiochem, San Diego, California ...

http://www.sciencepop.org/2018/02/cytoplasmic-linker-protein-clip-170-is-definitely-a-microtubule-mt-plus-end-tracking-protein/

https://wwwnc-origin.cdc.gov/eid/article/17/12/11-0607_article

This is part of a triplicate data set of non-overlapping fields of NIH 3T3 fibroblasts cultured on polystyrene. (cellimagelibrary.org)
These results suggest that ZIP kinase is critical for myosin phosphorylation and necessary for cell motile processes in mammalian fibroblasts. (rupress.org)
We found that adherence and spreading of NIH-3T3 fibroblasts was confined to PLL and collagen areas of the micropatterns. (strath.ac.uk)
The differing affinity of hepatocytes and NIH-3T3 fibroblasts for collagen and PLL patterns was used to develop a simple technique for creating a co-culture of the two cell types. (strath.ac.uk)
that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)

Western blot analysis of extracts from HeLa cells transfected with 100 nM control siRNA #6201 (-) or PAK1 siRNA, using PAK1 Antibody #2602 and p42 MAP Kinase (Erk2) Antibody #9108. (cellsignal.com)
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected EGFR in HeLa, A431, HUVEC, and NIH 3T3 cells. (sigmaaldrich.com)
RNAi/siRNA/Gene Knockdown Analysis: A 1:250 dilution from a representative lot detected EGFR lysates from wild type and EGFR knockout HeLa cells. (sigmaaldrich.com)
This gene was identified by its ability to suppress the tumorigenicity of Hela cells in nude mice. (nih.gov)
Differential expression of the human ST5 gene in HeLa-fibroblast hybrid cell lines mediated by YY1: evidence that YY1 plays a part in tumor suppression. (nih.gov)
Identification of a human chromosome 11 gene which is differentially regulated in tumorigenic and nontumorigenic somatic cell hybrids of HeLa cells. (nih.gov)
No. 556432) was used at 2 µg/ml to immunoprecipitate Cytochrome c from P388D1 mouse lymphoma (lane 2), HeLa human carcinoma (lane 3), Jurkat T leukemia (lane 4), and NIH/3T3 mouse fibroblast (lane 5) cell lysates. (bdbiosciences.com)
Immunofluorescent staining of HeLa (ATCC CCL-2) cells. (bdbiosciences.com)

Studies showing that murine fibroblast cell lines are susceptible to infection with mouse-adapted scrapie agent ( 11 , 12 ) increased concern that nonneuronal cell substrates used to propagate viruses for vaccine production might become infected with a TSE agent contaminating some component of culture medium, especially bovine serum ( 13 ). (cdc.gov)
The in vitro cytotoxicity studies of CS-IONPs and Chloramb-CS-IONPs nanocomposite were tested on the normal fibroblast cell lines (3T3) and leukemia cancer cell lines (WEHI). (dovepress.com)

Cytotoxicity was performed against Artemia salina and mouse embryonic fibroblast NIH/3T3 cell line following contact toxicity and MTT cells viability assays, respectively. (frontiersin.org)
An MTT assay was performed on two different cell lines (NIH-3T3 and MCF-7) to investigate the cytotoxicity profile. (elsevier.com)
Investigating the cytotoxicity profile of the target compound showed that all compounds 5a-o have IC 50 values higher than 50 µM for both tested cell lines. (elsevier.com)

Enhanced osteogenic potential of human mesenchymal stem cells on electrospun nanofibrous scaffolds prepared from eri-tasar silk fibroin. (semanticscholar.org)
The antiviral drugs G CV and P FA were loaded into the pH-responsive nanoparticles fabricated by poly(lactic-co-glycolic acid) ( P LGA) and 1,2-dioleoyl-3-trimethylammonium-propane ( D OTAP), and further coated with cell m embranes derived from bone marrow mesenchymal stem cells to form artificial stem cells, namely MPDGP . (researchsquare.com)
We also induced their adipogenic, osteogenic and chondrogenic differentiation, verifying their mesenchymal stem cells potentiality in vitro. (scielo.br)

This protein preferentially binds to the SH3 domain of c-Abl kinase, and acts as a regulator of MAPK1/ERK2 kinase, which may contribute to its ability to reduce the tumorigenic phenotype in cells. (nih.gov)

From reproduction and embryogenesis to cellular and tissular homeostasis in the adult, the control of metabolism and general physiology, as well as the dysregulations in pathological processes, cell communication is germane to all as. (researchgate.net)
To investigate whether a ketogenic diet might selectively impair energy metabolism in tumor cells, we characterized in vitro effects of the principle ketone body 3-hydroxybutyrate in rat hippocampal neurons and five glioma cell lines. (biomedcentral.com)
However, no activation of the hypoxia-inducible factor-1α (HIF-1α) pathway was observed in glioma cells, consistent with the absence of substantial 3-hydroxybutyrate metabolism and subsequent accumulation of succinate. (biomedcentral.com)

We demonstrate a single-step method for the generation of collagen and poly-l-Lysine (PLL) micropatterns on a poly(ethylene glycol) (PEG) functionalized glass surface for cell based assays. (strath.ac.uk)
The method involves establishing a reliable silanization method to create an effective non-adhesive PEG layer on glass that inhibits cell attachment, followed by the spotting of collagen or PLL solutions using non-contact piezoelectric printing. (strath.ac.uk)
In contrast, primary rat hepatocytes adhered and spread only on collagen micropatterns, where they formed uniform, well defined functionally active cell arrays. (strath.ac.uk)

We discovered that exposure of cell or tissue lysates to a biotinylated isoxazole (b-isox) chemical precipitated hundreds of RNA-binding proteins with significant overlap to the constituents of RNA granules. (nih.gov)

CIL:7829, Mus musculus, permanent cell line cell. (cellimagelibrary.org)
No PrP TSE or infectivity has been detected in any exposed cell line under study so far. (cdc.gov)
Note that the intensity of the fluorescence may vary, depending on the cell line and labeling substrate used. (neb.com)
Twenty four to 48 hours after transfection, begin selecting mammalian cultures in 600-1,200 µg/ml G418 (geneticin) depending on the cell line. (neb.com)
It is recommended that a kill curve be established for each cell line to determine optimal selection conditions. (neb.com)
PURPOSE: To propose an experimental burn model in NIH-3T3 cell line. (scielo.br)
Western Blot analysis of SP110 expression in transfected 293T cell line ( H00003431-T01 ) by SP110 MaxPab polyclonal antibody. (abnova.com)
A continuous cell line of high contact-inhibition established from NIH Swiss mouse embryo cultures. (bvsalud.org)

J Cell Biol (2004) 165 (2): 243-254. (rupress.org)
Mol Cell Biol. (exbio.cz)

In vitro transformation of C3H/lOTl/2 and NIH/3T3 cells by acrylonitrile and acrylamide. (cdc.gov)
CONCLUSION: The cells demonstrated adipogenic, osteogenic and chondrogenic differentiation potential in vitro. (scielo.br)
In summary, glioma cells are incapable of compensating for glucose restriction by metabolizing ketone bodies in vitro , suggesting a potential disadvantage of tumor cells compared to normal cells under a carbohydrate-restricted ketogenic diet. (biomedcentral.com)

ACP-ADRβ2 fusion protein gives a cell surface localized signal when labeled with CoA substrates. (neb.com)
It has been challenging to study the mechanism that drives the preferential migration of cells towards stiffer substrates at a rigidity border due to difficulties in capturing cells as they transiently encounter a rigidity interface. (cmu.edu)

Using mouse embryonic stem cells (mESCs) with a conditional knockout allele of Smc5 , we showed that Smc5 protein depletion resulted in destabilization of the Smc5/6 complex, accumulation of cells in G2 phase of the cell cycle and apoptosis. (biologists.com)

abstract = "The phenotypes of NIH 3T3 cells transfected with basic fibroblast growth factor (bFGF) cDNAs that express only the high molecular weight (HMW) forms of bFGF, the 18-kDa form, or all forms were examined. (elsevier.com)

The HM34 antibody does not stain bone marrow cells like some other mouse CD34 antibodies, probably because the antibody recognizes a different epitope from other mAbs. (biolegend.com)
Possible adhesion molecules thought to function in early hematopoiesis by mediating attachment of stem cells to bone marrow extracellular matrix or stromal cells. (biolegend.com)
Leukemia is one of the hematologic malignancies diseases which is most common in adults which affects the bone marrow, lymphatic system and blood cells. (dovepress.com)
1 Leukemia is clonal hematopoiesis created in the bone marrow and is discovered by the uncontrollable generation of poorly differentiated white blood cells. (dovepress.com)

Purpose: Caveolin (Cav) regulates various aspect of endothelial cell signaling and cell-permeable peptides (CPPs) fused to domains of Cav can reduce retinal damage and inflammation in vivo. (researchgate.net)
Diabetic patients are especially susceptible to oxidative stress and inflammatory diseases, because excessive glucose increases expression of endothelial cell nitric oxide synthase (eNOS) and the production of superoxide, leading to increased generation of hydroxyl radicals, hydrogen peroxide, and peroxynitrite [7-9]. (justia.com)

Primary CECs and mouse NIH 3T3 cells treated with the two FGF1 derivatives showed equivalent EC50 ranges (3.3-24 vs.1.9-16. (researchgate.net)
The mouse monoclonal antibody 16A1 recognizes an extracellular epitope of CD140a / PDGF-RA, the 170 kDa alpha chain of platelet-derived growth factor receptor, which is widely expressed on a variety of mesenchymal-derived cells and plays pro-proliferative or anti-proliferative roles in various tumours. (exbio.cz)

Additionally, tumor hypoxia results in constitutive upregulation of glycolysis and is considered to substantially contribute to the resistance of tumor cells to therapeutic strategies [ 4 , 5 ]. (biomedcentral.com)

We tested the global assays for their sensitivity to detect changes in signaling pathways in cells expressing cytoplasmic PrP mutants. (biomedcentral.com)

Approximately 200 MTs were analyzed in 10C20 control or exhausted cells for each experimental condition. (sciencepop.org)
The model experimental system allows efficient analyses of conditions affecting rigidity sensing of cells. (cmu.edu)

It is demonstrated that spider silk without any additional biological coating supports adhesion and growth of primary human urothelial cells (HUCs), which are multipotent bladder cells able to differentiate into the various epithelial layers of the bladder. (semanticscholar.org)
The sole receptor for the HGF ligand, MET (c-MET, mesenchymal-epithelial transition factor), is a transmembrane tyrosine kinase that is normally expressed in epithelial cells ( 1 ). (snmjournals.org)
CD140a has early developmental functions, mediates mesodermal cell migration, and later acts in signaling associated in epithelial-mesenchymal interactions. (exbio.cz)

This antibody also stained U-2 OS (ATCC HTB-96) and A549 (ATCC CCL-185) cells and worked with both the Triton™ X-100 and alcohol perm protocols (see Recommended Assay Procedure). (bdbiosciences.com)

2021. Cell Reports. (biolegend.com)

The present study develops a novel therapeutic intervention using artificial stem cells to deliver antiviral drugs at inflammatory sites, which shows great potential for the targeted treatment of pneumonia. (researchsquare.com)
To our best knowledge, we are the first to fabricate this kind of artificial stem cells to deliver the antiviral drugs for pneumonia treatment. (researchsquare.com)
The advances in stem cell therapy offer an alternative strategy for treating pneumonia. (researchsquare.com)
However, MSCs therapy also showed some limitations, such as the risk of pulmonary embolism induced by the micron-scale size, lack of large-scale supply of MSCs with "stem" properties, and when considering live cells as a delivery system, a limited drug loading capacity. (researchsquare.com)
CD34 is thought to function as an adhesion molecule for attachment of stem cells to extracellular matrix or stromal cells. (biolegend.com)
PURPOSE: To evaluate the effects of the adipose-derived stem cells (ADSC) in the viability of random skin flap in rats. (scielo.br)
The administration of adipose-derived stem cells was effective to increase the viability of the random skin flaps in rats. (scielo.br)
Correct duplication of stem cell genetic material and its appropriate segregation into daughter cells are requisites for tissue, organ and organism homeostasis. (biologists.com)
Disruption of stem cell genomic integrity can lead to developmental abnormalities and cancer. (biologists.com)
Roles of the Smc5/6 structural maintenance of chromosomes complex in pluripotent stem cell genome maintenance have not been investigated, despite its important roles in DNA synthesis, DNA repair and chromosome segregation as evaluated in other model systems. (biologists.com)
Our study reveals crucial requirements of the Smc5/6 complex during cell cycle progression and for stem cell genome maintenance. (biologists.com)
Pluripotent stem cells (PSCs) possess unlimited differentiation potential and are considered to be a source of cells for regenerative medicine. (biologists.com)

Western blot analysis of extracts from SH-SY5Y, C6 and NIH/3T3 cells, using PAK1 Antibody. (cellsignal.com)
After overnight incubation, cells were stained using the Triton™ X-100 perm protocol and the anti-Cytochrome C antibody. (bdbiosciences.com)
Separation of cells stained using anti-human CD140a (16A1) purified antibody (concentration in sample 0,33 μg/ml, GAM APC, red-filled) from cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining) of CD140a transfected cell suspension. (exbio.cz)
Western Blot: p19ARF/CDKN2A Antibody [NB200-106] - Analysis of p19ARF/CDKN2A in (A) NIH/3T3 and (B) MEF cells. (novusbio.com)
Immunocytochemistry/ Immunofluorescence: p19ARF/CDKN2A Antibody [NB200-106] - Antibody was tested in MEF cells with DyLight 488 (green). (novusbio.com)
YFP-tagged proteins were recognized by anti-GFP antibody on Western blot, and tubulin was discolored by silver-staining kit (Bio-Rad Laboratories, 207679-81-0 Hercules, CA). Measurements of Fluorescence in Cell Components Fluorescence resonance energy transfer (Stress) measurements were performed. (sciencepop.org)

Purpose: The purpose of the dataset was to measure the distribution of EGFP fluorescence intensities within individual cells in the population. (cellimagelibrary.org)
Labeling of transfected cells with a fluorescent CoA substrate should show strong surface fluorescence. (neb.com)

Expression of ACP-ADRβ2 can be achieved by transiently transfecting cells in culture with standard transfection protocols. (neb.com)
pACP-ADRβ2 has performed well in transient transfection of CHO-K1, COS-7, U-2 0S and NIH 3T3 cells. (neb.com)
Cell Lifestyle, Transfection, and Remedies Chinese language hamster ovary (CHO)-T1 cells had been grown up in Y-10 moderate, and COS-1 and NIH 3T3 cells early grown up in DMEM/Y-12 (1:1 mix) supplemented with 10% fetal bovine serum and antibiotics. (sciencepop.org)
The cells are useful for DNA transfection and transformation studies. (bvsalud.org)

NIH 3T3 and CHO-K1 cells had been treated with 10 g/ml Taxol (Sigma-Aldrich), 20 Meters forskolin, and 100 nM or 1 Meters OA for 1 l or with 40 Meters forskolin, 200 nM L-89, and 10 g/ml Taxol for 2 l. (sciencepop.org)

Hepatocyte growth factor (HGF), also called scatter factor, is a heterodimer protein that is normally synthesized by mesenchymal cells, including cancers such as sarcomas and high-grade gliomas ( 1 - 7 ). (snmjournals.org)
Deletion of the COOH terminus converts the ST5 p70 protein from an inhibitor of RAS signaling to an activator with transforming activity in NIH-3T3 cells. (nih.gov)
Expression of an isoform of the novel signal transduction protein ST5 is linked to cell morphology. (nih.gov)
To this end, we used as a model cytoplasmic mutants of the cellular prion protein (PrP C ), which are toxic to N2a neuroblastoma cells. (biomedcentral.com)
The mTOR signaling pathway regulates global protein synthesis, which is inhibited in cells expressing cytoplasmic PrP. (biomedcentral.com)
In addition, it has been suggested that the protein may play a role in ribosome biogenesis and in the induction of myeloid cell differentiation. (abnova.com)
The ketone body metabolizing enzymes 3-hydroxybutyrate dehydrogenase 1 and 2 (BDH1 and 2), 3-oxoacid-CoA transferase 1 (OXCT1) and acetyl-CoA acetyltransferase 1 (ACAT1) were expressed at the mRNA and protein level in all glioma cell lines. (biomedcentral.com)
The EVC2 protein is found in primary cilia, which are microscopic, finger-like projections that stick out from the surface of cells and are involved in signaling pathways that transmit information between cells. (medlineplus.gov)
In particular, the EVC2 protein is thought to help regulate a signaling pathway known as Sonic Hedgehog, which plays roles in cell growth, cell specialization, and the normal shaping (patterning) of many parts of the body. (medlineplus.gov)

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected EGFR in A431 cell lysate. (sigmaaldrich.com)

Utilising rabbit corneal endothelial cells (CEC) in three different paradigms, two human FGF1 derivatives (TTHX1001 and TTHX1114), engineered to exhibit greater stability, were tested as proliferative agents. (researchgate.net)

Western Blot analysis of SP110 expression in NIH/3T3. (abnova.com)

These cells tended to form multinucleated giant cells, did not grow in soft agar, were nontumorigenic, had a normal bFGF receptor number, and had a nontransformed morphology. (elsevier.com)
CD140a / PDGF-RA (platelet-derived growth factor receptor alpha) is a cell surface receptor for members of platelet-derived growth factor family, whose intracellular part contains a tyrosine kinase domain. (exbio.cz)

Cells expressing high levels of HMW bFGFs had a transformed morphology and were tumorigenic. (elsevier.com)

Native spider silk does not require any modification to its application as a biomaterial that can rival any artificial material in terms of cell growth promoting properties, and adhesion mechanics on intracellular level are shown. (semanticscholar.org)
Normally, ErbB-2 is expressed in a wide variety of tissues and is involved in normal growth, cell adhesion and development. (indianjcancer.com)
Focal adhesion kinase null (FAK -/-) cells, known to be defective in durotaxis, are able to readily cross the rigidity border, while reexpression of focal adhesion kinase (FAK) rescues rigidity sensing. (cmu.edu)

In addition, clonal cell lines can be isolated and characterized if desired. (neb.com)

Hierarchical and functional clustering of the relative expression levels identified an mTOR signaling pathway as potentially dysregulated in N2a cells expressing cytoplasmic PrP. (biomedcentral.com)

RESULTS: The ethanolic extract of Morinda citrifolia L. fruit (10mg/mL) decreased cellular activity and inhibited 45% the rate of cell proliferation of B16-F10 melanoma treated during period studied. (scielo.br)
Whereas CD140b induces in different cell types their proliferation and migration, the role of CD140a is more controversial, with pro-proliferative or anti-proliferative effects. (exbio.cz)

The third image is Texas Red C2-maleimide (to stain cell body), and the fourth image is Dapi (to stain nuclei). (cellimagelibrary.org)
3.) The number of nuclei in each ROI is determined from the DAPI image and the integrated intensity of the EGFP signal in the cell is determined from ROI in the EGFP image. (cellimagelibrary.org)
When this data is placed in a spread sheet, you can use the results to identify cell clusters (i.e. have more than 1 nuclei), debris or partial cell (i.e. no nuclei), poor EGFP/cell measurements (i.e. high background intensity). (cellimagelibrary.org)

Many investigators once believed that TSE agents infected mainly, if not exclusively, cells of neuronal and lymphoid lineages. (cdc.gov)

doi: 10.1016/j.cell.2012.04.017. (nih.gov)

Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)
genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)

The attachment of human articular cartilage cells were investigated on these bioprotein matrices. (semanticscholar.org)
In addition, these compounds were evaluated for their ability to compete for agonist and antagonist binding to cells expressing cloned human 5-HT2A, 5-HT2B, and 5-HT2C receptors. (erowid.org)

Cells producing the 18 kDa or all forms of bFGF were transformed at high levels of growth factor expression but were nontransformed at low levels. (elsevier.com)
It has become clear, however, that the susceptibility of cells to infection with TSE agents cannot be reliably predicted either from their tissue of origin or level of expression of PrP C ( 11 , 12 ). (cdc.gov)
and (c) disruption of ZIP kinase expression by RNA interference diminished myosin phosphorylation, and resulted in the defect of cell polarity and migration efficiency. (rupress.org)
It is possible to use pools of stable cell populations for initial cell labeling to test for the presence of ACP-tag expression. (neb.com)
This process is self-propagating, as the ROS released by inflammatory cells can increase the expression of leukocyte adhesive factors on the endothelium, resulting in local extravasation of additional leukocytes that produce additional ROS [5, 6]. (justia.com)

Obviously, other therapeutic approaches are needed that, on the one hand, impair tumor cell growth and, on the other hand, permit an adequate quality of life. (biomedcentral.com)

J Cell Sci (2016) 129 (8): 1619-1634. (biologists.com)

The staining solution was removed, cells were rinsed with PBS/3% BSA, and 0.05% sodium azide and PBS. (cellimagelibrary.org)

Reorganization of actomyosin is an essential process for cell migration and myosin regulatory light chain (MLC 20 ) phosphorylation plays a key role in this process. (rupress.org)
Additionally, I show that probing mechanisms at the front of a cell are used not only for probing rigidity but for sensing the state of migration. (cmu.edu)

In this study, we found that coffee extract at more than 2.5%(v/v) significantly inhibited LPS-induced inflammatory responses in RAW264.7 cells and that anti-inflammatory activity of coffee required the roasting process. (nature.com)
Using a model system developed for testing cellular responses at a simulated rigidity border, I find that NIH 3T3 cells preferentially localize to the rigid portion of the model system. (cmu.edu)

CONCLUSION: What these results indicate that the ethanolic extract of the fruit of Morinda citrifolia L. showed antitumor activity with inhibition of viability and growth of B16-F10 cells and also showed antibacterial activity as induced inhibition of growth of Staphylococcus aureus and Escherichia coli. (scielo.br)

Culture of melanoma cells B16-F10 for treatment with ethanolic extract of Morinda citrifolia L. fruit to determine cell viability by MTT and determination temporal effect of ethanolic extract fruit on the cell growth B16-F10 for 8 days. (scielo.br)

1.) The Txred images (which is a general purpose cell body stain) were segmented by manual thresholding. (cellimagelibrary.org)

These results suggest that in migrating cells continuous protrusion and mechanical probing directly in front of existing adhesions modulates traction force build up and serves as a key mechanism for regulating mechanical output in response to physical cues. (cmu.edu)

Cells use filopodia extensions to probe substrate rigidity in front of the leading edge and use substrate strain to determine whether the filopodia protrusions retract or expand to occupy the area. (cmu.edu)

Mechanical properties of the extracellular environment provide important cues that regulate cell behavior. (cmu.edu)

Anatomy8

Organisms8

Diseases4

Chemicals and Drugs41

Analytical, Diagnostic and Therapeutic Techniques and Equipment7

Psychiatry and Psychology1

Phenomena and Processes30

Disciplines and Occupations3

Anthropology, Education, Sociology and Social Phenomena4

Humanities1

Information Science8

Named Groups1

Health Care8

Publication Characteristics1

Geographicals1

Matrine inhibits production and actions of fibrogenic cytokines released by mouse peritoneal macrophages. (1/5011)

In vivo destabilization of dynamic microtubules by HDAC6-mediated deacetylation. (2/5011)

The formin-homology-domain-containing protein FHOD1 enhances cell migration. (3/5011)

Dynamic association of RNA-editing enzymes with the nucleolus. (4/5011)

Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail. (5/5011)

Antitumor effects of ZD6474, a small molecule vascular endothelial growth factor receptor tyrosine kinase inhibitor, with additional activity against epidermal growth factor receptor tyrosine kinase. (6/5011)

Specific inhibition of the Akt1 pleckstrin homology domain by D-3-deoxy-phosphatidyl-myo-inositol analogues. (7/5011)

Bfk: a novel weakly proapoptotic member of the Bcl-2 protein family with a BH3 and a BH2 region. (8/5011)

NIH 3T3 Cells

3T3 Cells

National Institutes of Health (U.S.)

Cell Transformation, Neoplastic

Consensus Development Conferences, NIH as Topic

Research Support as Topic

Transfection

Genes, ras

Oncogenes

Molecular Sequence Data

Cell Line

Base Sequence

Cell Line, Transformed

Cell Division

United States

Awards and Prizes

Oncogene Proteins v-raf

Oncogene Protein p21(ras)

Proto-Oncogene Proteins

National Library of Medicine (U.S.)

Amino Acid Sequence

Biomedical Research

Proto-Oncogene Proteins c-raf

Gene Expression Regulation

Retroviridae Proteins, Oncogenic

Cell Transformation, Viral

Proto-Oncogenes

Proto-Oncogene Proteins p21(ras)

Cloning, Molecular

Signal Transduction

Promoter Regions, Genetic

Moloney murine leukemia virus

Transcription, Genetic

Leukemia Virus, Murine

Phosphorylation

Harvey murine sarcoma virus

ras Proteins

Protein-Tyrosine Kinases

DNA

Mice, Inbred Strains

Retroviridae

RNA, Messenger

Recombinant Proteins

Mutation

Platelet-Derived Growth Factor

Enzyme Activation

Gene Expression

DNA-Binding Proteins

Plasmids

Cells, Cultured

Financing, Government

Sarcoma Viruses, Murine

Proteins

Transcription Factors

Research Personnel

Mice, Nude

Peer Review, Research

Budgets

Kirsten murine sarcoma virus

Genes, src

DNA, Complementary

Protein-Serine-Threonine Kinases

Consensus Development Conference, NIH

Cell Cycle

Calcium-Calmodulin-Dependent Protein Kinases

Tumor Cells, Cultured

Genetic Vectors

Mitogen-Activated Protein Kinases

Neoplasms, Experimental

Oncogene Proteins

Kinetics

Tetradecanoylphorbol Acetate

Nuclear Proteins

Cell Adhesion

Transformation, Genetic

Phenotype

Protein Binding

Oncogene Proteins v-mos

COS Cells

GTPase-Activating Proteins