A common neoplasm of early childhood arising from neural crest cells in the sympathetic nervous system, and characterized by diverse clinical behavior, ranging from spontaneous remission to rapid metastatic progression and death. This tumor is the most common intraabdominal malignancy of childhood, but it may also arise from thorax, neck, or rarely occur in the central nervous system. Histologic features include uniform round cells with hyperchromatic nuclei arranged in nests and separated by fibrovascular septa. Neuroblastomas may be associated with the opsoclonus-myoclonus syndrome. (From DeVita et al., Cancer: Principles and Practice of Oncology, 5th ed, pp2099-2101; Curr Opin Oncol 1998 Jan;10(1):43-51)
A cell line derived from cultured tumor cells.
A benign neoplasm that usually arises from the sympathetic trunk in the mediastinum. Histologic features include spindle cell proliferation (resembling a neurofibroma) and the presence of large ganglion cells. The tumor may present clinically with HORNER SYNDROME or diarrhea due to ectopic production of vasoactive intestinal peptide. (From DeVita et al., Cancer: Principles and Practice of Oncology, 5th ed, p966)
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Proteins coded by oncogenes. They include proteins resulting from the fusion of an oncogene and another gene (ONCOGENE PROTEINS, FUSION).
A guanidine analog with specific affinity for tissues of the sympathetic nervous system and related tumors. The radiolabeled forms are used as antineoplastic agents and radioactive imaging agents. (Merck Index, 12th ed) MIBG serves as a neuron-blocking agent which has a strong affinity for, and retention in, the adrenal medulla and also inhibits ADP-ribosyltransferase.
Family of retrovirus-associated DNA sequences (myc) originally isolated from an avian myelocytomatosis virus. The proto-oncogene myc (c-myc) codes for a nuclear protein which is involved in nucleic acid metabolism and in mediating the cellular response to growth factors. Truncation of the first exon, which appears to regulate c-myc expression, is crucial for tumorigenicity. The human c-myc gene is located at 8q24 on the long arm of chromosome 8.
A moderately malignant neoplasm composed of primitive neuroectodermal cells dispersed in myxomatous or fibrous stroma intermixed with mature ganglion cells. It may undergo transformation into a neuroblastoma. It arises from the sympathetic trunk or less frequently from the adrenal medulla, cerebral cortex, and other locations. Cervical ganglioneuroblastomas may be associated with HORNER SYNDROME and the tumor may occasionally secrete vasoactive intestinal peptide, resulting in chronic diarrhea.
A malignant olfactory neuroblastoma arising from the olfactory epithelium of the superior nasal cavity and cribriform plate. It is uncommon (3% of nasal tumors) and rarely is associated with the production of excess hormones (e.g., SIADH, Cushing Syndrome). It has a high propensity for multiple local recurrences and bony metastases. (From Holland et al., Cancer Medicine, 3rd ed, p1245; J Laryngol Otol 1998 Jul;112(7):628-33)
A selective increase in the number of copies of a gene coding for a specific protein without a proportional increase in other genes. It occurs naturally via the excision of a copy of the repeating sequence from the chromosome and its extrachromosomal replication in a plasmid, or via the production of an RNA transcript of the entire repeating sequence of ribosomal RNA followed by the reverse transcription of the molecule to produce an additional copy of the original DNA sequence. Laboratory techniques have been introduced for inducing disproportional replication by unequal crossing over, uptake of DNA from lysed cells, or generation of extrachromosomal sequences from rolling circle replication.
Benign and malignant neoplastic processes arising from or involving components of the central, peripheral, and autonomic nervous systems, cranial nerves, and meninges. Included in this category are primary and metastatic nervous system neoplasms.
A subclass of ACIDIC GLYCOSPHINGOLIPIDS. They contain one or more sialic acid (N-ACETYLNEURAMINIC ACID) residues. Using the Svennerholm system of abbrevations, gangliosides are designated G for ganglioside, plus subscript M, D, or T for mono-, di-, or trisialo, respectively, the subscript letter being followed by a subscript arabic numeral to indicated sequence of migration in thin-layer chromatograms. (From Oxford Dictionary of Biochemistry and Molecular Biology, 1997)
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.
Neoplasms located in the bone marrow. They are differentiated from neoplasms composed of bone marrow cells, such as MULTIPLE MYELOMA. Most bone marrow neoplasms are metastatic.
Tumors or cancer of the ADRENAL GLANDS.
An important regulator of GENE EXPRESSION during growth and development, and in NEOPLASMS. Tretinoin, also known as retinoic acid and derived from maternal VITAMIN A, is essential for normal GROWTH; and EMBRYONIC DEVELOPMENT. An excess of tretinoin can be teratogenic. It is used in the treatment of PSORIASIS; ACNE VULGARIS; and several other SKIN DISEASES. It has also been approved for use in promyelocytic leukemia (LEUKEMIA, PROMYELOCYTIC, ACUTE).
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.
Established cell cultures that have the potential to propagate indefinitely.
Any cell, other than a ZYGOTE, that contains elements (such as NUCLEI and CYTOPLASM) from two or more different cells, usually produced by artificial CELL FUSION.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Cellular DNA-binding proteins encoded by the c-myc genes. They are normally involved in nucleic acid metabolism and in mediating the cellular response to growth factors. Elevated and deregulated (constitutive) expression of c-myc proteins can cause tumorigenesis.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
A group of highly cellular primitive round cell neoplasms which occur extracranially in soft tissue and bone and are derived from embryonal neural crest cells. These tumors occur primarily in children and adolescents and share a number of characteristics with EWING SARCOMA.
Substances that inhibit or prevent the proliferation of NEOPLASMS.
A specific pair of human chromosomes in group A (CHROMOSOMES, HUMAN, 1-3) of the human chromosome classification.
In tissue culture, hairlike projections of neurons stimulated by growth factors and other molecules. These projections may go on to form a branched tree of dendrites or a single axon or they may be reabsorbed at a later stage of development. "Neurite" may refer to any filamentous or pointed outgrowth of an embryonal or tissue-culture neural cell.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
Neoplasms of the intracranial components of the central nervous system, including the cerebral hemispheres, basal ganglia, hypothalamus, thalamus, brain stem, and cerebellum. Brain neoplasms are subdivided into primary (originating from brain tissue) and secondary (i.e., metastatic) forms. Primary neoplasms are subdivided into benign and malignant forms. In general, brain tumors may also be classified by age of onset, histologic type, or presenting location in the brain.
Methods which attempt to express in replicable terms the extent of the neoplasm in the patient.
A synthetic retinoid that is used orally as a chemopreventive against prostate cancer and in women at risk of developing contralateral breast cancer. It is also effective as an antineoplastic agent.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations.
A topical dermatologic agent that is used in the treatment of ACNE VULGARIS and several other skin diseases. The drug has teratogenic and other adverse effects.
The systematic study of the complete DNA sequences (GENOME) of organisms.
Techniques of nucleotide sequence analysis that increase the range, complexity, sensitivity, and accuracy of results by greatly increasing the scale of operations and thus the number of nucleotides, and the number of copies of each nucleotide sequenced. The sequencing may be done by analysis of the synthesis or ligation products, hybridization to preexisting sequences, etc.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
A fibrous cord that connects the muscles in the back of the calf to the HEEL BONE.
A subspecialty of pathology concerned with the molecular basis (e.g., mutations) of various diseases.
The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell.
The thoracolumbar division of the autonomic nervous system. Sympathetic preganglionic fibers originate in neurons of the intermediolateral column of the spinal cord and project to the paravertebral and prevertebral ganglia, which in turn project to target organs. The sympathetic nervous system mediates the body's response to stressful situations, i.e., the fight or flight reactions. It often acts reciprocally to the parasympathetic system.
Cells from adult organisms that have been reprogrammed into a pluripotential state similar to that of EMBRYONIC STEM CELLS.
Cells that can give rise to cells of the three different GERM LAYERS.

Glycopeptides from the surgace of human neuroblastoma cells. (1/5127)

Glycopeptides suggesting a complex oligosaccharide composition are present on the surface of cells from human neuroblastoma tumors and several cell lines derived from the tumors. The glycopeptides, labeled with radioactive L-fucose, were removed from the cell surface with trypsin, digested with Pronase, and examined by chromatography on Sephadex G-50. Human skin fibroblasts, brain cells, and a fibroblast line derived from neuroblastoma tumor tissue show less complex glycopeptides. Although some differences exist between the cell lines and the primary tumor cells, the similarities between these human tumors and animal tumors examined previously are striking.  (+info)

Expression of the naturally occurring truncated trkB neurotrophin receptor induces outgrowth of filopodia and processes in neuroblastoma cells. (2/5127)

We have investigated the effects of the truncated trkB receptor isoform T1 (trkB.T1) by transient transfection into mouse N2a neuroblastoma cells. We observed that expression of trkB.T1 leads to a striking change in cell morphology characterized by outgrowth of filopodia and processes. A similar morphological response was also observed in SH-SY5Y human neuroblastoma cells and NIH3T3 fibroblasts transfected with trkB.T1. N2a cells lack endogenous expression of trkB isoforms, but express barely detectable amounts of its ligands, brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT-4). The morphological change was ligand-independent, since addition of exogenous BDNF or NT-4 or blockade of endogenous trkB ligands did not influence this response. Filopodia and process outgrowth was significantly suppressed when full-length trkB.TK+ was cotransfected together with trkB.T1 and this inhibitory effect was blocked by tyrosine kinase inhibitor K252a. Transfection of trkB.T1 deletion mutants showed that the morphological response is dependent on the extracellular, but not the intracellular domain of the receptor. Our results suggest a novel ligand-independent role for truncated trkB in the regulation of cellular morphology.  (+info)

p73 at chromosome 1p36.3 is lost in advanced stage neuroblastoma but its mutation is infrequent. (3/5127)

p73, a novel p53 family member, is a recently identified candidate neuroblastoma (NBL) suppressor gene mapped at chromosome 1p36.33 and was found to inhibit growth and induce apoptosis in cell lines. To test the hypothesis that p73 is a NBL suppressor gene, we analysed the p73 gene in primary human NBLs. Loss of heterozygosity (LOH) for p73 was observed in 19% (28/151) of informative cases which included 92 mass-screening (MS) tumors. The high frequency of p73 LOH was significantly associated with sporadic NBLs (9% vs 34%, P<0.001), N-myc amplification (10% vs 71%, P<0.001), and advanced stage (14% vs 28%, P<0.05). Both p73alpha and p73beta transcripts were detectable in only 46 of 134 (34%) NBLs at low levels by RT-PCR methods, while they were easily detectable in most breast cancers and colorectal cancers under the same conditions. They found no correlation between p73 LOH and its expression levels (P>0.1). We found two mutations out of 140 NBLs, one somatic and one germline, which result in amino acid substitutions in the C-terminal region of p73 which may affect transactivation functions, though, in the same tumor samples, no mutation of the p53 gene was observed as reported previously. These results suggest that allelic loss of the p73 gene may be a later event in NBL tumorigenesis. However, p73 is infrequently mutated in primary NBLs and may hardly function as a tumor suppressor in a classic Knudson's manner.  (+info)

Cadmium-mediated activation of the metal response element in human neuroblastoma cells lacking functional metal response element-binding transcription factor-1. (4/5127)

Metal response element-binding transcription factor-1 (MTF-1) binds specifically to metal response elements (MREs) and transactivates metallothionein (MT) gene expression in response to zinc and cadmium. This investigation contrasts the mechanism of mouse MT gene (mMT-I) promoter activation by cadmium and zinc in IMR-32 human neuroblastoma cells to determine whether MTF-1 binding to the MRE is necessary for activation by these metals. Cadmium activated a mMT-1 promoter (-150 base pairs) luciferase reporter 20-25-fold through a MRE-dependent mechanism. In contrast, zinc had little effect on the mMT-1 luciferase reporter. IMR-32 cells lacked MRE binding activity, and treatment with zinc in vitro or in vivo did not generate a MTF-1. MRE complex, suggesting that IMR-32 cells lack functional MTF-1. Overexpression of mMTF-1 regenerated a zinc-mediated induction of the MRE without affecting cadmium activation. Because no other transition metals tested activated the MRE, this effect appeared to be cadmium-specific. These data demonstrate that in IMR-32 human neuroblastoma cells, zinc and cadmium can use independent mechanisms for activation of the mMT-I promoter and cadmium-mediated MRE activation is independent of MTF-1 and zinc.  (+info)

Inhibition of angiogenesis induces chromaffin differentiation and apoptosis in neuroblastoma. (5/5127)

Inhibition of angiogenesis has been shown to reduce tumor growth, metastasis, and tumor microvascular density in experimental models. To these effects we would now like to add induction of differentiation, based on biological analysis of xenografted human neuroblastoma (SH-SY5Y, WAG rnu/rnu) treated with the angiogenesis inhibitor TNP-470. Treatment with TNP-470 (10 mg/kg s.c., n = 15) reduced the tumor growth by 66% and stereological vascular parameters (Lv, Vv, Sv) by 36-45%. The tumor cell apoptotic fraction increased more than threefold, resulting in a decrease in viable tumor cells by 33%. In contrast, the mean vascular diameter (29 microm) and the mean tumor cell proliferative index (49%) were unaffected. TNP-470-treated tumors exhibited striking chromaffin differentiation of neuroblastoma cells, observed as increased expression of insulin-like growth factor II gene (+88%), tyrosine hydroxylase (+96%), chromogranin A, and cellular processes. Statistical analysis revealed an inverse correlation between differentiation and angiogenesis. It is suggested that by inhibiting angiogenesis, TNP-470 induces metabolic stress, resulting in chromaffin differentiation and apoptosis in neuroblastoma. Such agonal differentiation may be the link between angiostatic therapy and tumor cell apoptosis.  (+info)

Binding partners for the myelin-associated glycoprotein of N2A neuroblastoma cells. (6/5127)

The myelin-associated glycoprotein (MAG) has been proposed to be important for the integrity of myelinated axons. For a better understanding of the interactions involved in the binding of MAG to neuronal axons, we performed this study to identify the binding partners for MAG on neuronal cells. Experiments with glycosylation inhibitors revealed that sialylated N-glycans of glycoproteins represent the major binding sites for MAG on the neuroblastoma cell line N2A. From extracts of [3H]glucosamine-labelled N2A cells several glycoproteins with molecular weights between 20 and 230 kDa were affinity-precipitated using immobilised MAG. The interactions of these proteins with MAG were sialic acid-dependent and specific for MAG.  (+info)

Comparison of two in vitro activation systems for protoxicant organophosphorous esterase inhibitors. (7/5127)

In order to perform in vitro testing of esterase inhibition caused by organophosphorous (OP) protoxicants, simple, reliable methods are needed to convert protoxicants to their esterase-inhibiting forms. Incubation of parathion or chlorpyrifos with 0.05% bromine solution or uninduced rat liver microsomes (RLM) resulted in production of the corresponding oxygen analogs of these OP compounds and markedly increased esterase inhibition in SH-SY5Y human neuroblastoma cells. Neither activation system affected cell viability or the activity of AChE or NTE in the absence of OP compounds. Although parathion and chlorpyrifos were activated by RLM, bromine activation required fewer steps and produced more esterase inhibition for a given concentration of chlorpyrifos. However, RLM activation of OP protoxicants produced metabolites other than oxygen analogs and may, therefore, be more relevant as a surrogate for OP biotransformation in vivo. This methodology makes the use of intact cells for in vitro testing of esterase inhibition caused by protoxicant organophosphate compounds a viable alternative to in vivo tests.  (+info)

MycN sensitizes neuroblastoma cells for drug-induced apoptosis. (8/5127)

Amplification of the MYCN gene is found in a large proportion of neuroblastoma and considered as an adverse prognostic factor. To investigate the effect of ectopic MycN expression on the susceptibility of neuroblastoma cells to cytotoxic drugs we used a human neuroblastoma cell line harboring tetracycline-controlled expression of MycN. Neither conditional expression of MycN alone nor low drug concentrations triggered apoptosis. However, when acting in concert, MycN and cytotoxic drugs efficiently induced cell death. Apoptosis depended on mitochondrial permeability transition and activation of caspases, since the mitochondrion-specific inhibitor bongkrekic acid and the caspase inhibitor zVAD-fmk almost completely abrogated apoptosis. Loss of mitochondrial transmembrane potential and release of cytochrome c from mitochondria preceded activation of caspase-8 and caspase-3 and cleavage of PARP. CD95 expression was upregulated by treatment with cytotoxic drugs, while MycN cooperated with cytotoxic drugs to increase sensitivity to CD95-induced apoptosis and enhancing CD95-L expression. MycN overexpression and cytotoxic drugs also synergized to induce p53 and Bax protein expression, while Bcl-2 and Bcl-X(L) protein levels remained unchanged. Since amplification of MYCN is usually associated with a poor prognosis, these findings suggest that dysfunctions in apoptosis pathways may be a mechanism by which MycN-induced apoptosis of neuroblastoma cells is inhibited.  (+info)

Neuroblastoma is the most common extracranial solid tumor in infants and children. Our lab and others have shown trophic actions of gastrin-releasing peptide (GRP), and its analogue bombesin (BBS), in neuroblastomas. Our lab also found that undifferentiated neuroblastomas express increased levels of GRP receptor (GRPR). Activation of the phosphatidylinositol-3-kinase (PI3K)/Akt pathway, a crucial regulator of cell survival, is associated with poor outcome in neuroblastomas and our lab¡¦s previous work has shown that GRPR regulates the expression of PI3K/Akt pathway components. However, the signaling mechanisms involved in this process are not clearly defined. Therefore, the objective of this project was to determine how GRP/GRPR, by way of PI3K pathway, regulates neuroblastoma growth. \r\n\r\nGRP/BBS treatment rapidly increased phosphorylation of both Akt and GSK-3ƒÒ in neuroblastoma cells. Antagonism or silencing of GRPR attenuated BBS-induced phosphorylation of Akt. PI3K inhibition also ...
Neuroblastoma is the most frequently diagnosed extracranial solid tumour in childhood. While a subset of tumours show spontaneous regression or complete remission following conventional treatment, a substantial number remain resistant to intensive multimodal therapies. Survival rates approaching 40% place high-risk neuroblastoma as one of the greatest challenges in paediatric oncology. This contemporary review provides an update on the diagnosis, risk stratification and management for this enigmatic tumour.. Neuroblastoma is the most common extracranial solid tumour in childhood and the most frequently diagnosed neoplasm during infancy.1 This malignant tumour consists of undifferentiated and/or differentiating cells originating from neural crest-derived sympathoadrenal precursors. Neuroblastoma is often described as enigmatic and unpredictable because of the broad spectrum of clinical behaviour ranging from life-threatening progression despite intensive treatment to complete spontaneous ...
TY - JOUR. T1 - Reversal of the effect of 12-O-tetradecanoyl-phorbol-13-acetate, TPA, on human neuroblastoma cells by nerve growth factor and mitomycin-C. AU - Goldstein, M. N.. AU - Malter, J. S.. PY - 1980. Y1 - 1980. UR - http://www.scopus.com/inward/record.url?scp=17544389097&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=17544389097&partnerID=8YFLogxK. M3 - Article. AN - SCOPUS:17544389097. VL - 87. JO - Journal of Cell Biology. JF - Journal of Cell Biology. SN - 0021-9525. IS - 2 II. ER - ...
TY - JOUR. T1 - Chromosomal aberrations and common fragile sites in neuroblastoma patients. AU - Vernole, P.. AU - Tedeschi, B.. AU - Pianca, C.. AU - Nicoletti, B.. AU - Riccardi, R.. AU - Melino, G.. PY - 1990. Y1 - 1990. N2 - We analyzed cytogenetically blood cells and bone marrow cells from 20 neuroblastoma patients. Chromosome common fragile sites were induced by aphidicolin in normal peripheral blood lymphocytes. All neuroblastoma patients showed a higher increase of aberrations after aphidicolin treatment as compared to that found in normal controls. In some cases it was possible to correlate the increase of the expression of a specific fragile site, 1p32, with deletions in the same area in bone marrow cells.. AB - We analyzed cytogenetically blood cells and bone marrow cells from 20 neuroblastoma patients. Chromosome common fragile sites were induced by aphidicolin in normal peripheral blood lymphocytes. All neuroblastoma patients showed a higher increase of aberrations after aphidicolin ...
Stage IV-S and International Neuroblastoma Staging System stage 4S were 98% concordant. MYCN was not amplified in any of the tumors tested (n = 58), and Shimada histopathologic classification was favorable in 96% (n = 68/71). The 5-year event-free survival (EFS) rate for all infants was 86% and the survival rate was 92%. Supportive care was the only treatment provided for 44 (55%) of 80 infants, and their 5-year survival rate was 100%, compared with 81% survival for those requiring cytotoxic therapy for symptoms (P =.005). Five of six deaths were in infants younger than 2 months of age at diagnosis and were due to complications of extensive abdominal involvement with respiratory compromise or disseminated intravascular coagulation. Although age ,/= 3 months at diagnosis was significant for EFS (P =. 043), it was less significant for survival (P =.077). The only other significant factor predictive for improved survival was favorable Shimada histopathologic classification. Sites of metastatic ...
Neuroblastoma is the most common extracranial solid tumor in childhood, with nearly 50% of patients presenting with widespread metastatic disease. The c
TY - JOUR. T1 - Regulation of tyrosine hydroxylase activity in mouse neuroblastoma clone N1E 115. AU - Richelson, E.. PY - 1973. Y1 - 1973. N2 - Mouse neuroblastoma (clone NIE 115) cells in the logarithmic growth phase were incubated for 12 days. From early log phase to late stationary phase, the specific activity of tyrosine hydroxylase (EC 1.14.3a) increased by more than 30 fold. The increase in tyrosine hydroxylase per cell and per dish was 12 and 2700 fold, respectively. When cell division was stopped by removing serum or by adding 0.1 mM 5 fluorodeoxyuridine and 0.1 mM uridine, the enzyme activity was also found to increase. These results show that tyrosine hydroxylase is regulated in neuroblastoma clone N1E 115.. AB - Mouse neuroblastoma (clone NIE 115) cells in the logarithmic growth phase were incubated for 12 days. From early log phase to late stationary phase, the specific activity of tyrosine hydroxylase (EC 1.14.3a) increased by more than 30 fold. The increase in tyrosine hydroxylase ...
TY - JOUR. T1 - Functional dissection of HOXD cluster genes in regulation of neuroblastoma cell proliferation and differentiation. AU - Zha, Yunhong. AU - Ding, Emily. AU - Yang, Liqun. AU - Mao, Ling. AU - Wang, Xiangwei. AU - McCarthy, Brian A.. AU - Huang, Shuang. AU - Ding, Hanfei. PY - 2012/8/7. Y1 - 2012/8/7. N2 - Retinoic acid (RA) can induce growth arrest and neuronal differentiation of neuroblastoma cells and has been used in clinic for treatment of neuroblastoma. It has been reported that RA induces the expression of several HOXD genes in human neuroblastoma cell lines, but their roles in RA action are largely unknown. The HOXD cluster contains nine genes (HOXD1, HOXD3, HOXD4, and HOXD8-13) that are positioned sequentially from 3′ to 5′, with HOXD1 at the 3′ end and HOXD13 the 5′ end. Here we show that all HOXD genes are induced by RA in the human neuroblastoma BE(2)-C cells, with the genes located at the 3′ end being activated generally earlier than those positioned more ...
eng] Stage 4S neuroblastoma (NB) is a special type of NB found in infants with metastases at diagnosis and is associated with an excellent outcome due to its remarkable capacity to undergo spontaneous regression. As genomics have not been able to explain this intriguing clinical presentation, we here aimed at profiling the DNA methylome of stage 4S NB to better understand this phenomenon. To this purpose, differential methylation analyses between International Neuroblastoma Staging System (INSS) stage 4S, stage 4 and stage 1/2 were performed, using methyl-CpG-binding domain (MBD) sequencing data of 14 stage 4S, 14 stage 4, and 13 stage 1/2 primary NB tumors (all MYCN non-amplified in order not to confound results). Stage 4S-specific hyper- and hypomethylated promoters were determined and further characterized for genomic localization and function by cytogenetic band enrichment, gene set enrichment, transcription factor target enrichment and differential RNA expression analyses. We show that ...
TY - JOUR. T1 - Detection of metastatic neuroblastoma in bone marrow biopsy specimens with an antibody to neuron-specific enolase.. AU - Crary, G. S.. AU - Singleton, T. P.. AU - Neglia, J. P.. AU - Swanson, P. E.. AU - Strickler, J. G.. PY - 1992/5. Y1 - 1992/5. N2 - To verify the practical utility of immunohistochemical analysis of bone marrow biopsy specimens in patients with neuroblastoma, we compared the results of routine histologic examination of 68 specimens with the results of immunohistochemical detection of tumor cells using an antibody to neuron-specific enolase (NSE). A commercially available polyclonal antibody to this enolase isoform consistently reacted with the neoplastic cells in biopsy specimens with histologic features diagnostic of (24 specimens) or suspicious for (one specimen) metastatic neuroblastoma. Immunohistochemical double-staining techniques documented that the NSE-positive neoplastic cells also reacted with antibodies to chromogranin and synaptophysin. Notably, ...
TY - JOUR. T1 - IGF-I receptor inhibition combined with rapamycin or temsirolimus inhibits neuroblastoma cell growth. AU - Coulter, Don W.. AU - Blatt, Julie. AU - DErcole, A. Joseph. AU - Moats-Staats, Billie M.. PY - 2008/5/1. Y1 - 2008/5/1. N2 - Background: Neuroblastoma is the third most common solid tumor in children. Treatment continues to be challenging. The pathogenesis of neuroblastoma has been related to expression of the type 1 insulin-like growth factor receptor (IGF1R) and to transcription factor MYC-N amplification. Previous studies have shown that MYC-N expression is disrupted by blockade of the IGF1R with a specific monoclonal antibody, αIR3. Inhibition of IGF1R signaling can be accomplished by other agents, including rapamycin or temsirolimus, which target mTOR (mammalian target of rapamycin). Materials and Methods: BE-2(c) and IMR-32 neuroblastoma cell lines were treated with varying concentrations of αIR3, rapamycin and temsirolimus alone or in combination and the viable ...
Abstract. Neuroblastoma (NBL) is the most common extracranial solid tumor of childhood, with about 700 new cases of neuroblastoma seen each year in the United States. The 5-year survival rate for children with high-risk NBL is only 50-60%, and this survival rate has not improved over the last 10 years. High-risk patients receive multimodality treatment, including chemotherapy, surgery, radiation therapy, biologic therapy and immunotherapy, all of which are associated with significant morbidity. Recent years have seen many advances in treatment of neuroblastoma, including therapeutic MIBG, immunotherapy, and personalized targeted therapy based on the genetic alterations seen in the tumor. The primary objective of this book is to provide the readers with a comprehensive review of neuroblastoma, from clinical aspects and the currently available treatment to recent advancements and future directions in the field of NBL treatment. The topics and chapters have been compiled keeping in mind a diverse ...
Neuroblastoma is the most common extracranial solid tumor of infancy. It is an embryonal malignancy of the sympathetic nervous system arising from neuroblasts (pluripotent sympathetic cells).
Melphalan could prolong the survival of children with advanced neuroblastoma and is currently used in many high-dose protocols for this patient group (20). The present study shows for the first time that J1, a prodrug of melphalan, is highly active against human neuroblastoma cell lines in vitro and in vivo. The cytotoxic activity of J1 in vitro was found to be superior compared with melphalan, and statistical comparisons also favored J1 in terms of antiproliferative, proapoptotic, and antiangiogenic activity in vivo. These findings are particularly interesting in view of the demonstrated clinical activity of Peptichemio in neuroblastoma (4, 5).. Despite different levels of absolute sensitivity, the seven human neuroblastoma cell lines displayed a similar pattern of sensitivity against the tested drugs (Pearson correlation of log IC50, 0.85-0.99), reflecting the importance of a common cytopathologic origin. The in vitro activity of J1 was, on average, 270-fold that of melphalan, with a range ...
VACCINE DOSING: Vaccine components SJNB-JF-IL2 and SJNB-JF-Lptn will each be dosed at 1x10e7 cells/m2. This will be given in conjunction with an escalating dose of SKNLP vaccine in the phase I portion of this study. In the phase II portion of this study, the same dose of SJNB-JF-IL2 and SJNB-JF-Lptn will be given in conjunction with the highest dose of SKNLP determined in the phase I portion. Vaccination will be administered on an inpatient or outpatient basis. Patient will be notified of which dose of vaccine cells they will receive if enrolled in the study.. Phase I Dose Escalation Component: While the investigators do not suspect that addition of a second irradiated, unmodified neuroblastoma tumor cell line to the previously tested SJNB-JF gene modified cell line will affect the safety profile of the vaccine, as the SKNLP has not been tested previously in vaccine studies, the investigators will perform an abbreviated dose escalation study of the combined vaccine to assess safety. The ...
TY - JOUR. T1 - Glucose metabolism in mixed glioblastoma and neuroblastoma cultures. AU - Newburgh, R. W.. AU - Rosenberg, Roger N.. PY - 1973/5/15. Y1 - 1973/5/15. N2 - When glioblastoma and neuroblastoma cells are mixed, an inhibition of 14CO2 evolution from [1-14C]-glucose occurs. This does not occur when Hela and Glioblastoma cells or Hela and neuroblastoma cells are mixed. Mixing the cells has no effect on the incorporation of [1-14C] glucose.. AB - When glioblastoma and neuroblastoma cells are mixed, an inhibition of 14CO2 evolution from [1-14C]-glucose occurs. This does not occur when Hela and Glioblastoma cells or Hela and neuroblastoma cells are mixed. Mixing the cells has no effect on the incorporation of [1-14C] glucose.. UR - http://www.scopus.com/inward/record.url?scp=0015789342&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0015789342&partnerID=8YFLogxK. U2 - 10.1016/0006-291X(73)90757-2. DO - 10.1016/0006-291X(73)90757-2. M3 - Article. C2 - 4351142. AN - ...
In the present study, we provide evidence that freshly isolated neuroblastoma cells are susceptible to NK-mediated lysis. More importantly, we show that a key role in the lytic process is played by PVR, a molecule expressed at the tumor cell surface that is recognized by the DNAM-1 receptor. We analyzed highly purified, fresh neuroblastoma cells isolated from bone marrow aspirates (22) . As compared with cultured neuroblastoma cell lines (20) , freshly isolated neuroblasts were generally more resistant to lysis. Remarkably, a certain degree of variability existed among different tumors. In particular, we show that tumor cells displaying maximal susceptibility to lysis were characterized by high surface expression of PVR. This molecule was recently recognized as a ligand for DNAM-1, a surface receptor mediating NK cell activation and tumor cell killing (17) . In line with these findings, we now demonstrate that mAb-mediated disruption of DNAM-1-PVR interactions inhibits NK-mediated killing of ...
Rhabdomyosarcoma (RMS) is the third most common extracranial solid tumor of childhood. Approximately 350 new cases are diagnosed in the United States each year accounting for 3 percent of childhood cancers. RMS is derived from primitive myoblasts and morphologically resembles early stages of prenatal skeletal muscle differentiation. However, a large percentage of RMS tumors occur in locations normally lacking skeletal muscle, with the head and neck, genitourinary tract and retroperitoneum being frequent sites of tumor localization. Development of RMS has been associated with genetic tumor predisposition syndromes including Li-Fraumeni syndrome, neurofibromatosis and Costello syndrome. Childhood RMS is subdivided into two major subtypes, embryonal and alveolar, which have distinct histological features and genetic alterations. Adult RMS is largely a third histological subtype, namely pleomorphic RMS. The alveolar RMS subtype carries a poorer prognosis and is strongly myogenin positive by ...
Rhabdomyosarcoma (RMS) is the third most common extracranial solid tumor of childhood. Approximately 350 new cases are diagnosed in the United States each year accounting for 3 percent of childhood cancers. RMS is derived from primitive myoblasts and morphologically resembles early stages of prenatal skeletal muscle differentiation. However, a large percentage of RMS tumors occur in locations normally lacking skeletal muscle, with the head and neck, genitourinary tract and retroperitoneum being frequent sites of tumor localization. Development of RMS has been associated with genetic tumor predisposition syndromes including Li-Fraumeni syndrome, neurofibromatosis and Costello syndrome. Childhood RMS is subdivided into two major subtypes, embryonal and alveolar, which have distinct histological features and genetic alterations. Adult RMS is largely a third histological subtype, namely pleomorphic RMS. The alveolar RMS subtype carries a poorer prognosis and is strongly myogenin positive by ...
Purpose: Neuroblastoma is the most common and deadly solid tumor of childhood. Cyclooxygenase-2 is expressed in clinical neuroblastoma tumors and cell lines and inhibitors of this enzyme induce apoptosis in human neuroblastoma cells in vitro and in neuroblastoma xenografts in vivo. We hypothesized that the cyclooxygenase-2- specific inhibitor celecoxib could enhance the cytotoxic effect of chemotherapeutic drugs currently used in neuroblastoma treatment. Furthermore, we investigated if prophylactic treatment with celecoxib could prevent neuroblastoma tumor development in vivo. Experimental Design: Neuroblastoma cell cytotoxicity of chemotherapeutic drugs in combination with celecoxib was examined. In vivo, athymic rats carrying established SH-SY5Y xenografts were treated with celecoxib in combination with irinotecan, doxorubicin or etoposide, or with either drug alone. For prevention studies, rats received celecoxib in the diet, 250 to 2,500 ppm, from the time of tumor cell injection. Results: ...
Neuroblastoma is a childhood extracranial solid tumour that is associated with a number of genetic changes. Included in these genetic alterations are mutations in the kinase domain of the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase (RTK), which have been found in both somatic and familial neuroblastoma. In order to treat patients accordingly requires characterisation of these mutations in terms of their response to ALK tyrosine kinase inhibitors (TKIs). Here, we report the identification and characterisation of two novel neuroblastoma ALK mutations (A1099T and R1464STOP), which we have investigated together with several previously reported but uncharacterised ALK mutations (T1087I, D1091N, T1151M, M1166R, F1174I and A1234T). In order to understand the potential role of these ALK mutations in neuroblastoma progression, we have employed cell culture-based systems together with the model organism Drosophila as a readout for ligand-independent activity. Mutation of ALK at position 1174 (F1174I
Epidemiological and preclinical studies have revealed that omega-3 fatty acids have anticancer properties. We have previously shown that the omega-3 fatty acid docosahexaenoic acid (DHA) induces apoptosis of neuroblastoma cells in vitro by mechanisms involving intracellular peroxidation of DHA by means of 15-lipoxygenase or autoxidation. In our study, the effects of DHA supplementation on neuroblastoma tumor growth in vivo were investigated using two complementary approaches. For the purpose of prevention, DHA as a dietary supplement was fed to athymic rats before the rats were xenografted with human neuroblastoma cells. For therapeutic purposes, athymic rats with established neuroblastoma xenografts were given DHA daily by gavage and tumor growth was monitored. DHA levels in plasma and tumor tissue were analyzed by gas liquid chromatography. DHA delayed neuroblastoma xenograft development and inhibited the growth of established neuroblastoma xenografts in athymic rats. A revised version of the ...
OUTLINE: This is a multicenter study.. Previously collected samples are analyzed to define the genome-wide DNA copy number and allelic status; to define the genome-wide methylation profile of high-risk neuroblastoma cases; to define the genome-wide microRNA expression profile of high-risk neuroblastoma cases; to define the genome-wide RNA expression and relating gene expression to DNA copy number and gene polymorphisms, DNA methylation, and microRNA expression; to resequence three genomes: the neuroblastoma genome, the transcriptome, and the paired constitutional genome; and to characterize the relapsed high-risk neuroblastoma genome and epigenome.. PROJECTED ACCRUAL: A total of 300 tumor samples from patients with high-risk disease, 50 tumor samples from patients with low-risk primary neuroblastoma, and 30 human neuroblastoma-derived cell lines will be accrued for this study. ...
At the ASCO Plenary Session where the HR-NBL1/SIOPEN trial was presented, formal discussant Julie R. Park, MD, of the University of Washington, Seattle, said, Large randomized trials have previously shown that myeloablative therapy improves outcomes in high-risk neuroblastoma, and it is now considered standard of care.. Dr. Park explained that the COJEC regimen used in this study may have contributed to the lower-than-expected 3-year event-free survival in the CEM arm. Previous studies conducted by the Childrens Oncology Group achieved a 3-year event-free survival of 46% with CEM, but those studies used a different induction regimen with lower doses of cisplatin and etoposide and did not include carboplatin.. It is possible that rapid COJEC had a negative interaction with CEM, but not BuMel, she said.. The SIOPEN trial is a great achievement and shows the unity of 20 European nations. This study confirms the importance of myeloablative therapy in a cohort of high-risk neuroblastoma with a ...
TY - JOUR. T1 - Treatment of high-risk neuroblastoma with triple-tandem high-dose therapy and stem-cell rescue. T2 - Results of the Chicago Pilot II study. AU - Kletzel, Morris. AU - Katzenstein, Howard M.. AU - Haut, Paul R.. AU - Yu, Alice L.. AU - Morgan, Elaine. AU - Reynolds, Marleta. AU - Geissler, Grant. AU - Marymount, Maryanne H.. AU - Liu, Dachao. AU - Kalapurakal, John A.. AU - Shore, Richard M.. AU - Bardo, Diana M.E.. AU - Schmoldt, Jennifer. AU - Rademaker, Alfred W.. AU - Cohn, Susan L.. PY - 2002/5/1. Y1 - 2002/5/1. N2 - Purpose: To investigate whether intensive induction therapy followed by triple-tandem cycles of high-dose therapy with peripheral-blood stem-cell rescue and local irradiation will improve event-free survival for patients with high-risk neuroblastoma. Patients and Methods: From August 1995 to January 2000, 25 consecutive newly diagnosed high-risk neuroblastoma patients and one child with recurrent MYCN-amplified disease were enrolled onto the Chicago Pilot II ...
Vella, S.; Conti, M.; Tasso, R.; Cancedda, R.; Pagano, A., 2012: Dichloroacetate inhibits neuroblastoma growth by specifically acting against malignant undifferentiated cells
Prenylated Rab acceptor 1 domain family, member 2 (PRAF2) is a novel 19-kDa protein with four transmembrane-spanning domains that belongs to the PRAF protein family. Neuroblastoma (NB) is the most common malignant extracranial solid tumor of childhood that originates in primitive cells of the developing sympathetic nervous system. We investigated the correlation of PRAF2 mRNA expression to NB clinical and genetic parameters using Affymetrix expression analysis of a series of 88 NB tumors and examined the functional role of PRAF2 in an NB cell line using RNA interference. We found that high PRAF2 expression is significantly correlated to several unfavorable NB characteristics: MYCN amplification, high age at diagnosis, poor outcome and high INSS stage. The shRNA-mediated PRAF2 downregulation in the SK-N-SH NB cell line resulted in decreased cellular proliferation, migration and matrix-attachment. These findings were confirmed in NB patient tumor samples, where high PRAF2 expression is ...
Recently, we showed that high T-cell infiltration correlates with favorable clinical outcome in high-risk neuroblastoma patients (13). Herein, we demonstrate that TILs express PD-1 and LAG3, two negative regulators of T-cell function (33), and that neuroblastoma cells either express PD-L1 or HLA-I. According to the density of PD-L1+ and HLA-I+ tumor cells and regardless of infiltrating T-cell density, MYCN amplification status, INSS stage, and age at diagnosis, we could distinguish two PD-L1/HLA-I combinations: one associated with good prognosis (high HLA-I and low or no PD-L1) and the other associated with poor prognosis (low HLA-I and high or no PD-L1). Our findings provide a proof of principle that PD-L1 in combination with HLA-I expression may serve as a biomarker in neuroblastoma, although this has to be further confirmed by a prospective study with a larger number of samples.. Of note, a good PD-L1/HLA-I combination was also associated with favorable clinical outcome in intrahepatic ...
July 5, 2011 - A new treatment option may soon be available for children with neuroblastoma, according to research published in the July issue of The Journal of Nuclear Medicine. The study tested the principle that combined positron emission tomography and X-ray computed tomography (PET/CT) may be used to select children with primary refractory or relapsed high-risk neuroblastoma for treatment with a molecular radiotherapy known as 177Lu-DOTATATE. This therapeutic option was found to be a viable option for children with neuroblastomas. Neuroblastoma is a cancerous tumor that develops from nerve tissue in infants and children. Accounting for six to 10 percent of all childhood cancers, it does not always follow the same pattern, with some patients regressing spontaneously and other progressing, despite aggressive therapy. The long-term survival rate for neuroblastoma is below 40 percent. We know that peptide receptor radionuclide therapy in adults with somatostatin-positive neuroendocrine tumors ...
TY - JOUR. T1 - Phase II study of temozolomide in combination with topotecan (TOTEM) in relapsed or refractory neuroblastoma. T2 - A European Innovative Therapies for Children with Cancer-SIOP-European Neuroblastoma study. AU - Di Giannatale, Angela. AU - Dias-Gastellier, Nathalie. AU - Devos, Annick. AU - Mc Hugh, Kieran. AU - Boubaker, Ariane. AU - Courbon, Frederic. AU - Verschuur, Arnaud. AU - Ducassoul, Stéphane. AU - Malekzadeh, Katty. AU - Casanova, Michela. AU - Amoroso, Loredana. AU - Chastagner, Pascal. AU - Zwaan, Christian M.. AU - Munzer, Caroline. AU - Aerts, Isabelle. AU - Landman-Parker, Judith. AU - Riccardi, Riccardo. AU - Le Deley, Marie Cecile. AU - Geoerger, Birgit. AU - Rubie, Hervé. PY - 2014/1. Y1 - 2014/1. N2 - Purpose To assess objective response rate (ORR) after two cycles of temozolomide in combination with topotecan (TOTEM) in children with refractory or relapsed neuroblastoma. Patients and Methods This multicenter, non-randomised, phase II study included children ...
The nature of the signal transducing the change in cell volume to the activation of osmolyte efflux pathways for RVD in osmotically swollen cells is not clear. Two major models have been proposed, one involving Ca2+ as an intracellular second messenger and the other in which RVD is mediated through Ca2+-independent mechanisms. According to the first model, cell swelling causes an increase in cytosolic Ca2+ that activates volume regulatory mechanisms. The increase in [Ca2+]i may result from Ca2+ influx, via plasma membrane channels (Christensen, 1987), or Ca2+ release from intracellular stores (McCarty and ONeil, 1992; Wu et al., 1997). The proposed effectors are mostly Ca2+-activated cation and anion channels and carriers. This model has been widely accepted as a paradigm for the involvement of Ca2+ as a transducing signal for RVD. Nevertheless, the existing evidence backing up this model is often weak and fragmentary, and the comprehensive analysis necessary to establish an active role of ...
TY - JOUR. T1 - Transplantation of a temperature-sensitive, nerve growth factor-secreting, neuroblastoma cell line into adult rats with fimbria-fornix lesions rescues cholinergic septal neurons. AU - Whittemore, S. R.. AU - Holets, V. R.. AU - Keane, Robert. AU - Levy, D. J.. AU - McKay, R. D G. PY - 1991/1/1. Y1 - 1991/1/1. N2 - The HT4 cell line was derived from infection of a mouse neuroblastoma cell line with a retrovirus that encoded the temperature-sensitive (ts) mutant of SV40 large T antigen. At nonpermissive temperature, HT4 cells differentiated with neuronal morphology, expressed neuronal antigens, synthesized nerve growth factor (NGF) mRNA, and secreted biologically active NGF in vitro. We sought to establish whether transplanted HT4 cells expressed class I major histocompatibility to complex (MHC) antigens, a partial requirement for recognition by cytotoxic T lymphocytes (CTL), and thus be susceptible to xenograft rejection. Differentiated HT4 cells expressed marginally detectable ...
Purpose: Neuroblastoma is the most common extracranial solid cancer type in childhood and high-risk patients have poor prognosis despite aggressive multimodal treatment. Neuroblastoma-driven inflammation contributes to the induction of suppressive myeloid cells that hamper efficient anti-tumor immune responses. Therefore, we sought to enhance anti-tumor immunity by removing immunosuppression mediated by myeloid cells. Experimental Design: The prognostic values of myeloid cells are demonstrated by analyzing genomic datasets of neuroblastoma patients. The impact of tumor-derived factors on myelopoiesis and local induction of suppressive myeloid cells are dissected by in vitro culture models using freshly isolated human CD34+ hematopoietic stem cells, primary human monocytes and murine bone marrow cells. To test the therapeutic efficacy of BLZ945 as a monotherapy or in combination with checkpoint inhibitors, we employed a transgenic murine model (TH-MYCN) that develops aggressive spontaneous ...
Neuroblastomas are the most common extracranial solid childhood malignancies and the third commonest childhood tumors after leukemia and brain malignancies. The tumors typically occur in infants and very young children (mean age of presentation being ~22 months) with 95% of cases diagnosed before the age of 10 years.. Clinical presentation. Typically with pain or a palpable mass and abdominal distension, although numerous other presentations may be encountered due to local mass effect.. Location. Neuroblastomas arise from the sympathetic nervous system 2-3:. Intra-abdominal disease (two-thirds of cases) is more prevalent than the intrathoracic disease. Specific sites include:. ...
Ewing sarcoma is an aggressive bone tumor that occurs in children and young adults. Cure rates, particularly when disease has spread, are low with currently available treatments. Dr. Guenther aims to identify critical genes on which Ewing sarcoma cells are dependent for survival, with the goal of discovering weaknesses in these cancer cells that may be exploited to stop cancer growth. CITED2 is of particular interest as a Ewing sarcoma-specific dependency gene based on a genome-wide screen in hundreds of cancer cell lines. In some other cancers, CITED2 is described as important for helping cells repair damage and survive stress, such as when they are exposed to chemotherapy. She has found that CITED2 is present in higher levels in Ewing sarcoma cells than in other types of cancer, and when CITED2s function is turned off in Ewing sarcoma cells, they grow more slowly. She aims to first confirm that CITED2 is critical for Ewing sarcoma survival. She will also investigate what makes CITED2 ...
Hydroxybutyrate (GHB) is an endogenous metabolite synthesized in the brain. There is strong evidence to suggest that GHB has an important role as a neurotransmitter or neuromodulator.. The human aldo-keto reductase AKR7A2 has been proposed previously to catalyze the NADPH-dependent reduction of succinic semialdehyde (SSA) to GHB in human brain. In this study we have used RNA interference to evaluate the role of AKR7A2 in GHB biosynthesis in human neuroblastoma SH-SY5Y cells. Quantitative reverse transcription-PCR analysis and immunoblotting revealed that short interfering RNA molecules directed against AKR7A2 led to a significant reduction in both AKR7A2 transcript and protein levels 72 h post-transfection. We have shown that reduced expression of AKR7A2 results in a 90% decrease in SSA reductase activity of cell extracts. Furthermore, we have shown using gas chromatography-mass spectrometry that a decrease in the level of AKR7A2 was paralleled with a significant reduction in intracellular GHB ...
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Neuroblastoma is a cancerous tumor found in young children and infants, most commonly in children under age 5 and rarely in those older than 10. Approximately 650 children in the United States are diagnosed with neuroblastoma each year.. What causes neuroblastoma?. The cause of neuroblastoma is not certain, but researchers believe it forms from neuroblasts, immature nerve cells, that grow abnormally in the fetus due to a genetic mutation. In most neuroblastoma cases, scientists cannot identify the genetic abnormality or its cause.. Neuroblastoma often begins in the adrenal glands on top of the kidneys, but it also can begin in the chest, neck, or pelvis. In more than 70 percent of diagnosed cases, neuroblastoma tumors have metastasized, or spread, to other areas of the body such as the bones, bone marrow, lymph nodes, and liver.. ...
Histone methylation plays an important role in gene transcription and chromatin organization and is linked to the silencing of a number of critical tumor suppressor genes in tumorigenesis. G9a is a histone methyltransferase (HMTase) for histone H3 lysine 9. In this study, we investigated the role of G9a in neuroblastoma tumor growth together with the G9a inhibitor BIX01294. The exposure of neuroblastoma cells to BIX01294 resulted in the inhibition of cell growth and proliferation, and BIX01294 treatment resulted in the inhibition of the tumorigenicity of neuroblastoma cells in NOD/SCID mice. Therefore, G9a may be a potential therapeutic target in neuroblastoma. Moreover, we found several specific characteristics of autophagy after BIX01294 treatment, including the appearance of membranous vacuoles and microtubule-associated protein light chain 3 (LC3B). Similar results were observed in G9a-knockdown cells. In conclusion, our results demonstrated that G9a is a prognostic marker in neuroblastoma, and
Survival and distribution of transplanted human IMR-32 neuroblastoma cells. Article date: 1991/12/24 PubMed ID: 1814581 Journal name: Brain research (ISSN: 0006-8993) ABSTRACT The visualisation of transplanted cell lines is essential to determine both their viability and possible functional properties. Fluorescent latex microspheres were used to label cultured human neuroblastoma IMR-32 cells prior to transplantation. IMR-32 cells were first rendered amitotic by treatment with mitom…
Cancer therapy development has improved the survival rate of patients over the past few decades. The major limitation of this approach is the inevitable emergence of resistance upon patient relapse. Acquired drug resistance can develop over the course of treatment causing tumours to no longer respond to therapy. There are several mechanisms that have been proposed to contribute to this development. Platinum compounds, a major class of chemotherapy agents, are used in approximately half of all chemotherapy schedules. Cisplatin is an alkylating-like agent that causes DNA damage by binding to purine bases and forming DNA lesions, eventually leading to cell death. Regardless of cisplatins efficacy, there two major limitations for its use, severe cytotoxic side effects and the inevitable formation of drug resistance. For this project, two neuroblastoma cell lines UKF-NB-3 and UKF-NB-6, derived from late-stage high-risk neuroblastoma patients, have been adapted to cisplatin in order to investigate ...
Two Baylor College of Medicine physicians have received support from the nonprofit group Cookies for Kids Cancer for their research on neuroblastoma - a common type of solid tumor in children.. Dr. Leonid Metelitsa, associate professor of pediatrics at BCM and a pediatric oncologist at Texas Childrens Cancer Center, and Dr. Jed Nuchtern, professor of pediatrics and of surgery at BCM, who is a surgical oncologist at the center, each received $100,000 from the organization.. The funding supports their work to find new treatments for neuroblastoma. The survival rate for neuroblastoma is currently less than 40 percent, and it accounts for 15 percent of all pediatric cancer deaths.. Still difficult to treat. The overall survival rate of childrens cancers has improved to about 80 percent today from 10 percent 30 years ago but the fact remains that some cancers, including some forms of neuroblastoma, are still very hard to treat, said Dr. David Poplack, director of Texas Childrens Cancer Center, ...
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Green, R D. and Stanberry, L R., Elevation of cyclic amp in c-1300 murine neuroblastoma by adenosine and related compounds and the antagonism of this response by methylxanthines. (1977). Subject Strain Bibliography 1977. 1535 ...
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PubMed journal article: Chlorpyrifos and cypermethrin induce apoptosis in human neuroblastoma cell line SH-SY5Y. Download Prime PubMed App to iPhone, iPad, or Android
TY - JOUR. T1 - Effects of β-sheet breaker peptide polymers on scrapie-infected mouse neuroblastoma cells and their affinities to prion protein fragment PrP(81-145). AU - Oishi, Takehisa. AU - Hagiwara, Ken Ichi. AU - Kinumi, Tomoya. AU - Yamakawa, Yoshio. AU - Nishijima, Masahiro. AU - Nakamura, Kazuhiko. AU - Arimoto, Hirokazu. PY - 2003/8/7. Y1 - 2003/8/7. N2 - Effects of β-sheet breaker peptide polymers on scrapie-infected mouse neuroblastoma cells and their affinities to prion protein fragment PrP (81-145) were studied. The dissociation constant was found to be fairly large, which provided evidence against the initial hypothesis. Surface plasmon resonance study indicated that direct binding between PrP(81-145) and the β-sheet breaker peptide was not specific and may not play a major role in the inhibition of PrP formation.. AB - Effects of β-sheet breaker peptide polymers on scrapie-infected mouse neuroblastoma cells and their affinities to prion protein fragment PrP (81-145) were ...
Periodical: McGee, Richard, Paul Simpson, Clifford Christian, Marina Mata, Phillip Nelson, and Marshall W. Nirenberg. Regulation of Acetylcholine Release from Neuroblastoma x Glioma Hybrid Cells. Proceedings of the National Academy of Sciences of the United States of America 75, 3 (March 1978): 1314-1318. Article. 5 Images ...
Neuroblastoma is an extracranial solid tumor that mainly occurs in children. It is derived from embryonic neural crest cells of the peripheral sympathetic nervous system. The overall survival rate of malignant neuroblastoma patients is very poor despite multi-modal therapy including surgery, radiotherapy, and chemotherapy. Efficacy of chemotherapy is often compromised due to presence of autophagy, which is a survival mechanism in solid tumors. Autophagy is a catablolic process for lysosomal degradation of cytoplasmic contents for recycling and it is activated during stress such as nutrient starvation and growth factor deprivation. The hallmark of autophagy is generation of double membrane structure called autophagosome that contains the microtubule-associated protein light chain 3 form II (LC3 II). In this study, we used rapamycin to mimic starvation-induced autophagy in human malignant neuroblastoma SK-N-BE2 and IMR32 cells and then investigated capability of the combination of LC3 II knockdown ...
TY - JOUR. T1 - Age, Diagnostic Category, Tumor Grade, and Mitosis-Karyorrhexis Index Are Independently Prognostic in Neuroblastoma. T2 - An INRG Project. AU - Sokol, Elizabeth. AU - Desai, Ami V.. AU - Applebaum, Mark A.. AU - Valteau-Couanet, Dominique. AU - Park, Julie R.. AU - Pearson, Andrew D.J.. AU - Schleiermacher, Gudrun. AU - Irwin, Meredith S.. AU - Hogarty, Michael. AU - Naranjo, Arlene. AU - Volchenboum, Samuel. AU - Cohn, Susan L.. AU - London, Wendy B.. PY - 2020. Y1 - 2020. N2 - PURPOSE The Childrens Oncology Group (COG) stratifies the treatment of patients with neuroblastoma on the basis of a combination of biomarkers that include age and tumor histology classified by age-linked International Neuroblastoma Pathology Classification (INPC) criteria. By definition, this leads to a duplication of the prognostic contribution of age. The individual histologic features underlying the INPC have prognostic strength and are incorporated in the International Neuroblastoma Risk Group ...
TY - JOUR. T1 - Synergy of topotecan in combination with vincristine for treatment of pediatric solid tumor xenografts. AU - Thompson, Joyce. AU - George, E. Olusegun. AU - Poquette, Catherine A.. AU - Cheshire, Pamela J.. AU - Richmond, Lois B.. AU - De Graaf, Siebold S.N.. AU - Ma, Margaret. AU - Stewart, Clinton F.. AU - Houghton, Peter J.. PY - 1999/11/1. Y1 - 1999/11/1. N2 - Topotecan and vincristine were evaluated alone or in combination against 13 independent xenografts and 1 vincristine-resistant derivative, representing childhood neuroblastoma (n = 6), rhabdomyosarcoma (n = 5), or brain tumors (n = 3). Topotecan was given by i.v. bolus on a schedule found previously to be optimal. Drug was administered daily for 5 days on 2 consecutive weeks with cycles repeated every 21 days over a period of 8 weeks. Doses of topotecan ranged from 0.16 to 1.5 mg/kg to simulate clinically achievable topotecan lactone plasma systemic exposures. Vincristine was administered i.v. every 7 days at a fixed ...
Paraspinal Neuroblastoma vs. Adrenal Gland Neuroblastoma. Paraspinal Neuroblastoma vs. Adrenal Gland Neuroblastoma Left picture: From the coarse and irregular calcifications in the x-ray a retroperitoneal teratoma can be expected; in fact, a paraspinal neuroblastoma was present in this case. Right picture: The diagnosis in this 11-month-old infant is a neuroblastoma of the right adrenal gland with skull metastasis. The operative preparation shows multiple scattered calcifications corresponding to the multiple fine and wispy calcifications of the tumor x-rays, and which are pathognomonic for a retroperitoneal neuroblastoma, which is illustrated best by the third picture from the top. Left picture: Lateral picture of a contrast enema in a girl just after infancy; the colon is displaced forward, and some relatively large, coarse calcifications are recognizable in the retroperitoeal space. Right picture: 11-month-old boy with a palpable mass in the right upper belly and a protrusion of the bulbi. In ...
This clone is cross reactive with non-human primate neuroblastoma, including inositol triphosphate receptors I and III (< 0.01; < 0.001) (Physique 1Ai). IMR-32 neuroblastoma cells were more sensitive to CDDP, showing a significant decrease in cell viability after treatment with 10 M CDDP for 24 h (< 0.05); 1 and 10 M CDDP for 48 h (< 0.05 and < 0.001) and 72 h (< 0.001 and < 0.001) (Physique 1Bi). A third neuroblastoma cell line, NLF, was less sensitive to CDDP, i.e., exhibited a significant decrease in cell viability only after 48h treatment with 10 M CDDP (< 0.001; Supplementary Physique 1). Physique 1 Cell survival and apoptosis in neuroblastoma cells following CDDP or TOPO treatment TOPO (0.1 nM to 1 1 M) demonstrated a stronger cytotoxic effect compared to CDDP in all neuroblastoma cell lines tested and cell viability was significantly reduced in SH-SY5Y cell after 24 h, 48 h and 72 h of exposure (Determine 1Ai). The cytotoxic effects of TOPO were stronger in IMR-32 cells as compared with ...
There is currently no curative treatment for children with relapsed/refractory neuroblastoma, and for these children the 5 year survival rate is
Neuroblastomas express increased levels of gastrin-releasing peptide receptor (GRP-R). However, the exact molecular mechanisms involved in GRP-R-mediated cell signaling in neuroblastoma growth and metastasis are unknown. Here, we report that focal adhesion kinase (FAK), as a critical downstream target of GRP-R, is an important regulator of neuroblastoma tumorigenicity. We found that FAK expression correlates with GRP-R expression in human neuroblastoma sections and cell lines. GRP-R overexpression in SK-N-SH cells increased FAK, integrin α3 and β1 expressions and cell migration. These cells demonstrated flatter cell morphology with broad lamellae, in which intense FAK expression was localized to the leading edges of lamellipodia. Interestingly, FAK activation was, in part, dependent on integrin α3 and β1 expression. Conversely, GRP-R silencing decreased FAK as well as Mycn levels in BE(2)-C cells, which displayed a denser cellular morphology. Importantly, rescue experiments in GRP-R silenced BE(2)-C
Human neuroblastoma (NB) cell lines may present with either one of the so-called S-and N-subtypes. We have previously reported a strong correlation between protein expression levels of vimentin, an S-subtype marker, and the p21Waf1 cyclin-dependent kinase inhibitor. We here investigated whether this correlation extend to the mRNA level in NB cell lines as well as in patients tumors. We also further explored the relationship between expression of vimentin and p21, by asking whether vimentin could regulate p21 expression. Vimentin and p21 mRNA levels in NB cell lines as well as in patients tumors (n = 77) were quantified using Q-PCR. Q-PCR data obtained from tumors of high risk NB patients (n = 40) were analyzed in relation with the overall survival using the Log-rank Kaplan-Meier estimation. siRNA-mediated depletion or overexpression of vimentin in highly or low expressing vimentin cell lines, respectively, followed by protein expression and promoter activation assays were used to assess the role of
Looking for online definition of neuroblastoma in the Medical Dictionary? neuroblastoma explanation free. What is neuroblastoma? Meaning of neuroblastoma medical term. What does neuroblastoma mean?
Neuroblastoma x glioma hybrid NG108-15 cells endogenously express at least three receptors which activate adenylate cyclase via the intermediacy of the stimulatory G-protein, Gs. Sustained exposure of the cells to agonists at the IP prostanoid receptor results in a substantial decrease in cellular levels of the alpha-subunit of Gs (Gs alpha) [McKenzie and Milligan (1990) J. Biol. Chem. 265, 17084-17093; Adie, Mullaney, McKenzie and Milligan (1992) Biochem J. 285, 529-536]. By contrast, equivalent treatments of the cells with agonists at either the A2 adenosine receptor or the secretin receptor have no measurable effect on cellular amounts of Gs alpha. To examine whether this is a feature specific to the IP prostanoid receptor or is related to the level of expression of the individual receptors, NG108-15 cells were transfected with a construct containing a human beta 2-adrenoceptor cDNA under the control of the beta-actin promoter. Two clones of these cells were examined in detail, beta N22, ...
TY - JOUR. T1 - Mitochondria produce reactive nitrogen species via an arginine-independent pathway. AU - Lacza, Zsombor. AU - Kozlov, Andrey V.. AU - Pankotai, Eszter. AU - Csordás, Attila. AU - Wolf, Gerald. AU - Redl, Heinz. AU - Kollai, Márk. AU - Szabo, Csaba. AU - Busija, David W.. AU - Horn, Thomas F W. PY - 2006/4. Y1 - 2006/4. N2 - We measured the contribution of mitochondrial nitric oxide synthase (mtNOS) and respiratory chain enzymes to reactive nitrogen species (RNS) production. Diaminofluorescein (DAF) was applied for the assessment of RNS production in isolated mouse brain, heart and liver mitochondria and also in a cultured neuroblastoma cell line by confocal microscopy and flow cytometry. Mitochondria produced RNS, which was inhibited by catalysts of peroxynitrite decomposition but not by nitric oxide (NO) synthase inhibitors. Disrupting the organelles or withdrawing respiratory substrates markedly reduced RNS production. Inhibition of complex I abolished the DAF signal, which ...
undecylenic acid.. Now scientists from Poland have done an experiment to improve the efficiency of neuroprotective effects of undecylenic acid by targeting it using polyelectrolyte coated nanocapsules. Core shell nanocapsules were prepared using nanoemulsification and the layer by layer technique. The average size of nanocapsules was around 80nm and the concentration was 2.5×1010 particles/ml. The nanocapsules were coated with either polycation or polyanion layers.. Biocompatibility of the synthesized nanocarriers was evaluated in SH-SY5Y human neuroblastoma cell line using cell viability/toxicity assays.. Results showed that nanocapsules coated with PLL and PGA were non-toxic to the human neuroblastoma cell line, thereby showing its potential to be used as a carrier of undecylenic acid. Further research showed that nanoencapsulated form of undecylenic acid was biocompatible and more effective against staurosporine-induced damage in lower concentrations than those of the same drug added ...
Its primary physiological function is unclear. Has cytoprotective activity against internal or environmental stresses. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or ZN(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains (By similarity).
Its primary physiological function is unclear. Has cytoprotective activity against internal or environmental stresses. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or ZN(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains (By similarity).
In most mammalian cells, regulatory volume decrease (RVD) is mediated by swelling-activated Cl(-) and K(+) channels. Previous studies in the human neuroblastoma cell line CHP-100 have demonstrated that exposure to hypoosmotic solutions activates Cl(-) channels which are sensitive to Ca(2+). Whether a Ca(2+)-dependent K(+) conductance is activated after cell swelling was investigated in the present studies. Reducing the extracellular osmolarity from 290 to 190 mOsm/kg H(2)O rapidly activated 86Rb effluxes. Hypoosmotic stress also increased cytosolic Ca(2+) in fura-2 loaded cells.
The role of reactive oxygen species (ROS) in the regulation of signal transduction processes has been well established in many cell types and recently the fine tuning of redox signalling in neurons received increasing attention. With regard to this, the involvement of NADPH oxidase (NOX) in neuronal pathophysiology has been proposed but deserves more investigation. In the present study, we used SH-SY5Y neuroblastoma cells to analyse the role of NADPH oxidase in retinoic acid (RA)-induced differentiation, pointing out the involvement of protein kinase C (PKC) delta in the activation of NOX. Retinoic acid induces neuronal differentiation as revealed by the increased expression of MAP2, the decreased cell doubling rate, and the gain in neuronal morphological features and these events are accompanied by the increased expression level of PKC delta and p67(phox), one of the components of NADPH oxidase. Using DPI to inhibit NOX activity we show that retinoic acid acts through this enzyme to induce ...
TY - JOUR. T1 - Leflunomide Reduces Proliferation and Induces Apoptosis in Neuroblastoma Cells In Vitro and In Vivo. AU - Zhu, Shunqin. AU - Yan, Xiaomin. AU - Xiang, Zhonghuai. AU - Ding, Han Fei. AU - Cui, Hongjuan. PY - 2013/8/9. Y1 - 2013/8/9. N2 - Leflunomide as an immunosuppressive drug is generally used in the treatment of rheumatoid arthritis. It inhibits DHODH (dihydroorotate dehydrogenase), which is one of the essential enzymes in the de novo pyrimidine biosynthetic pathway. Here we showed that leflunomide significantly reduced cell proliferation and self-renewal activity. Annexin V-FITC/PI staining assay revealed that leflunomide induced S-phase cell cycle arrest, and promoted cell apoptosis. In vivo xenograft study in SCID mice showed that leflunomide inhibited tumor growth and development. We also observed that DHODH was commonly expressed in neuroblastoma. When treated with leflunomide, the neuroblastoma cell lines BE(2)-C, SK-N-DZ, and SK-N-F1 showed dramatic inhibition of DHODH ...
Publisher: University of Delaware. Date Issued: 2014. Abstract: This study was designed to examine the mechanism by which inhibition of lysosomal proteases causes cell death in neuroblastoma. The major lysosomal proteases are two cysteine proteases, cathepsins B and L, and an aspartic protease, cathepsin D. Inhibition of these three proteases was found to cause cellular accumulation of fragments of the IGF-1 receptor. The fragments were located in dense organelles that were characterized as autophagosomes. This novel discovery provides the first clear link between lysosIGF-1omal function, autophagy and IGF-1 mediated cell proliferation. It provides a mechanistic explanation for enhanced cytotoxicity of chemotherpautic agents when combined with inhibitors of lysosomal function and autophagy. A more in depth analysis of the IGF1 signaling pathway revealed that the MAPK pathway was particularly impaired in inhibitor treated cells, while the PKB cell survival pathway remained functional. It was ...
TY - JOUR. T1 - Identification and characterization of a 500-kb homozygously deleted region at 1p36.2-p36.3 in a neuroblastoma cell line. AU - Ohira, Miki. AU - Kageyama, Hajime. AU - Mihara, Motohiro. AU - Furuta, Shigeyuki. AU - Machida, Taiichi. AU - Shishikura, Tomotane. AU - Takayasu, Hajime. AU - Islam, Ashraful. AU - Nakamura, Yohko. AU - Takahashi, Masato. AU - Tomioka, Nobumoto. AU - Sakiyama, Shigeru. AU - Kaneko, Yasuhiko. AU - Toyoda, Atsushi. AU - Hattori, Masahira. AU - Sakaki, Yoshiyuki. AU - Ohki, Misao. AU - Horii, Akira. AU - Soeda, Eiichi. AU - Inazawa, Johji. AU - Seki, Naohiko. AU - Kuma, Hidekazu. AU - Nozawa, Iwao. AU - Nakagawara, Akira. PY - 2000/8/31. Y1 - 2000/8/31. N2 - Loss of heterozygosity of the distal region of chromosome 1p where tumor suppressor gene(s) might harbor is frequently observed in many human cancers including neuroblastoma (NBL) with MYCN amplification and poor prognosis. We have identified for the first time a homozygously deleted region at the ...
The cell signaling docking protein p130cas became tyrosine-phosphorylated in SH-SY5Y human neuroblastoma cells during induced differentiation with 12-O-tetradecanoylphorbol-13-acetate (TPA) and serum or a combination of basic fibroblast growth factor (bFGF) and insulin-like growth factor-I (IGF-I). The differentiating cells develop a neuronal phenotype with neurites and growth cones and sustained activation of protein kinase C (PKC) and pp60c-src. The TPA-induced p130cas phosphorylation increased within 5 min of stimulation and persisted for at least 4 days, whereas bFGF/IGF-I-induced p130cas phosphorylation was biphasic. However, the increase in tyrosine phosphorylation of p130cas was not restricted to differentiation inducing stimuli. The phosphorylation was blocked by the specific PKC inhibitor GF 109203X, and transient transfection with active PKC-epsilon induced p130cas tyrosine phosphorylation. pp60c-src, known to directly phosphorylate p130cas in other cell systems, was not activated ...
Hello! The term neuroblastoma is commonly used to refer to a spectrum of neuroblastic tumors (including neuroblastomas, ganglioneuroblastomas, and ganglioneuromas) that arise from primitive sympathetic ganglion cells and, like paragangliomas and pheochromocytomas, have the capacity to synthesize and secrete catecholamines.. Remember the N myc mnemonic for neuroblastoma :. N - Neuroblastoma, N myc ...
NEUROBLASTOMA IS THE MOST COMMON, HIGHLY MALIGNANT, SOLID TUMOR FOUND IN CHILDREN. WHEN DIAGNOSED IN ASYMPTOMATIC CHILDREN, THE PROGNOSIS IS OFTEN POOR. HOWEVER, WHEN DETECTED IN ASYMPTOMATIC CHILDREN AS A RESULT OF THE URINE TUMOR MARKERS SCREENING PROGRAM, THE NEUROBLASTOMA IS USUALLY LOCALIZED AND TREATABLE, AND THE OUTCOME IS FAVORABLE. MOST NEUROBLASTOMA CASES HAVE HIGH URINE LEVELS OF HOMOVANILLIC ACID (HVA) AND/OR VANILLYLMANDELIC ACID (VMA). THESE TWO TUMOR MARKERS CAN BE MEASURED ACCURATELY AND PRECISELY BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY OR GAS CHROMATOGRAPHY, BUT THESE PROCEDURES ARE NOT PRACTICAL FOR DETERMINING HVA AND VMA IN LARGE NUMBERS OF URINE SPECIMENS. THIS PROJECT WILL DEVELOP A UNIQUE ENZYME IMMUNOASSAY (EIA) METHOD WITH MONOCLONAL ANTIBODIES TO DETECT HVA AND VMA. THE TECHNIQUE WILL BE AUTOMATED, COMPUTERIZED, AND COST EFFECTIVE, PERMITTING THE SCREENING OF A LARGE NUMBER OF SPECIMENS. THE EIA METHOD FOR HVA AND VMA WILL BE SPECIFIC AND SENSITIVE ENOUGH TO DETECT ...
International Neuroblastoma Research and Collaboration for Effective Delivery (INBRACED) is a new collaboration between charities, researchers and clinicians with the shared aim of accelerating the development of new, more effective therapies for the childhood cancer neuroblastoma.Central to the groups activities is the introduction of standardised, international clinical trials.
Sigma-Aldrich offers abstracts and full-text articles by [Ivan S Alferiev, Radhika Iyer, Jamie L Croucher, Richard F Adamo, Kehan Zhang, Jennifer L Mangino, Venkatadri Kolla, Ilia Fishbein, Garrett M Brodeur, Robert J Levy, Michael Chorny].
BACKGROUND: Although disease remission can frequently be achieved for patients with neuroblastoma, relapse is common. The cancer stem cell theory suggests that rare tumorigenic cells, resistant to conventional therapy, are responsible for relapse. If true for neuroblastoma, improved cure rates may only be achieved via identification and therapeutic targeting of the neuroblastoma tumor initiating cell. Based on cues from normal stem cells, evidence for tumor populating progenitor cells has been found in a variety of cancers. METHODOLOGY/PRINCIPAL FINDINGS: Four of eight human neuroblastoma cell lines formed tumorspheres in neural stem cell media, and all contained some cells that expressed neurogenic stem cell markers including CD133, ABCG2, and nestin. Three lines tested could be induced into multi-lineage differentiation. LA-N-5 spheres were further studied and showed a verapamil-sensitive side population, relative resistance to doxorubicin, and CD133+ cells showed increased sphere formation ...
Chemotherapy is one of the major modalities in treating cancers. However, its effectiveness is limited by the acquisition of multidrug resistance (MDR). Several mechanisms could explain the up-regulation of MDR genes/proteins in cancer after chemotherapy. It is known that cancer stem cells (CSCs) play a role as master regulators. Therefore, understanding the mechanisms that regulate some traits of CSCs may help design efficient strategies to overcome chemoresistance. Different CSC phenotypes have been identified, including those found in some pediatric malignancies. As solid tumors in children significantly differ from those observed in adults, this review aims at providing an overview of the mechanistic relationship between MDR and CSCs in common solid tumors, and, in particular, focuses on clinical as well as experimental evidence of the relations between CSCs and MDR in neuroblastoma and hepatoblastoma. Finally, some novel approaches, such as concomitant targeting of multiple key transcription
In this study, an efficient purification method for the polyphenols of Pinus koraiensis pinecone (PPP) has been developed. AB-8 resin was verified to offer good adsorption and desorption ratio for PPP. Response surface methodology (RSM) indicated that the optimized purification parameters for PPP were 1.70 mg GAE/mL phenolic sample concentration, 22.00 mL sample volume, and 63.00% ethanol concentration. Under these conditions, the experimental purity of PPP was 27.93 ± 0.14% (n = 3), which matched well with the predicted purity of 28.17%. Next, the antiproliferative effects of PPP on seven cancer cell lines, including A375 (human skin melanoma cancer cell line), A549 (human lung cancer cell line), SH-SY5Y (human neuroblastoma cell line), LOVO (human colon cancer stem cell line), MCF-7 (human breast cancer cell line), HeLa (human cervical cancer line), and HT29 (human colon cancer line), were examined by MTT assays. The results indicated that PPP had the highest capacity for inhibiting LOVO cells growth
Neuroblastoma Cell Lines Contain Pluripotent Tumor Initiating Cells That Are Susceptible to a Targeted Oncolytic Virus. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Attaching curcumin, a component of the common spice turmeric, to nanoparticles can be used to target and destroy treatment-resistant neuroblastoma tumor cells, according to a new study published in Nanoscale.
Villablanca JG, Volchenboum SL, Cho H, Kang MH, Cohn SL, Anderson CP, Marachelian A, Groshen S, Tsao-Wei D, Matthay KK, Maris JM, Hasenauer CE, Czarnecki S, Lai H, Goodarzian F, Shimada H, Reynolds CP. A Phase I New Approaches to Neuroblastoma Therapy Study of Buthionine Sulfoximine and Melphalan With Autologous Stem Cells for Recurrent/Refractory High-Risk Neuroblastoma. Pediatr Blood Cancer. 2016 08; 63(8):1349-56 ...
The purpose of this research study is to evaluate an investigational drug (DFMO) in combination with bortezomib, for relapsed and refractory neuroblastoma. DFMO is an investigational drug because it has not been approved by the U.S. Food and Drug Administration (FDA). This study will look at the safety and tolerability of DFMO in combination with bortezomib as well as the tumors response to this study drug.
Some organophosphorus compounds (OP), including the pesticide mipafox, produce late onset distal axonal degeneration, known as organophosphorus-induced delayed neuropathy (OPIDN). The underlying mechanism involves irreversible inhibition of neuropathy target esterase (NTE) activity, elevated intracellular calcium levels, increased activity of calcium-activated proteases and impaired neuritogenesis. Voltage-gated calcium channels (VGCC) appear to play a role in several neurologic disorders, including OPIDN. Therefore, this study aimed to examine and compare the neuroprotective effects of T-type (amiloride) and L-type (nimodipine) VGCC blockers induced by the inhibitory actions of mipafox on neurite outgrowth and axonal proteins of retinoic-acid-stimulated SH-SY5Y human neuroblastoma cells, a neuronal model widely employed to determine the neurotoxicity attributed to OP ...
Background. The Quebec Neuroblastoma Screening Project was initiated to assess clinical and biologic aspects of neuroblastomas detected by screening infants born in the province of Quebec from May 1,1989, to April 30,1994. Methods. Infants were screened for preclinical detection of neuroblastoma by determination of catecholamine metabolites,...
The investigation of nuclear encoded mitochondrial proteins has been simplified due to an abundance of methods for manipulating the nuclear genome. However, methods for studying the effects of mutations found in mitochondrial DNA (mtDNA) are limited and indirect, resulting in a significant gap in our ability to address fundamental questions about mitochondrial contribution to disease. In an attempt to bridge this gap, we used an RNA localization sequence derived from H1 RNA, previously discovered by Koehler and Teitell to deliver mRNA of native mtCO2 and mtCO3 to mitochondria for translation in situ. Our results indicate successful import and translation of these mRNAs within the mitochondrial matrix of SH-SY5Y human neuroblastoma cells. We are currently developing this method as a means for functional rescue of mtDNA-encoded neurological and mitochondrial defects in mtATP8 mutant mice and cells by targeting nucleus-transcribed mitochondrial mRNA transcripts to mitochondria for translation and ...
Euxanthone, a potent neuritogenic compound isolated from the roots of the medicinal herb POLYGALA CAUDATA, has recently been shown to induce the differentiation of murine neuroblastoma Neuro 2A (BU-1) cells. In this study, the role of protein kinase C (PKC) and the expression of various PKC... mehr ...
Cells of the murine neuroblastoma clone N1E-115 possess muscarinic receptors that influence the intracellular level of cyclic nucleotides. The stimulation of [3H]cyclic GMP levels occurs only with intact cells and has an EC50 near the low-affinity agonist equilibrium dissociation constant (KL) determined by radioligand binding assays. The inhibition of prostaglandin E1-stimulated [3H]cyclic AMP formation has an EC50 close to the value for the high-affinity agonist equilibrium dissociation constant (KH). During sequential subculturing in medium supplemented with newborn bovine serum, the inhibition of [3H]cyclic AMP was maintained, but the [3H]cyclic GMP response declined dramatically, and after 7 subculturings it was essentially absent. The time course for [3H]cyclic GMP formation in a late subculture with an 88% loss of the response was identical with the time course in early subcultures. A normal [3H]cyclic GMP response to bradykinin and histamine was demonstrated to be present in cells ...
The most common form of extra-cranial solid tumor in children is neuroblastoma, which represents 8% to 10% of all childhood ... The median age for the diagnosis of neuroblastoma is 22 months, being rare in adolescence and adulthood but showing poor ... The degree of differentiation of neuroblastoma is correlated to the prognosis, with a wide variety of outcomes (from tumor ... Colon NC, Chung DH (January 2011). "Neuroblastoma". Advances in Pediatrics. 58 (1): 297-311. doi:10.1016/j.yapd.2011.03.011. ...
Thompson LD (2009). Olfactory neuroblastoma. Head and neck pathology. 3: 252-259 Jiang, G. Y., Li, F. C., Chen, W. K., Liu, A. ... A report from the TREP project in cooperation with the Italian Neuroblastoma and Soft tissue sarcoma Committees. BMC Cancer ... Ghaffar, S., & Salahuddin, I. (2005). Olfactory neuroblastoma: a case report and review of the literature. Ear, nose, & throat ... Unger F, Walch C, Stammberger H, Papaefthymiou G, Haselsberger K, Pendl G (2001). "Olfactory neuroblastoma ( ...
Taylorville was known (in the early to mid-1990s) to have had a high rate of neuroblastoma, a cancer affecting the adrenal ... Neuroblastoma Coal Tar Cancer. Clutterinvestigations.com. Retrieved on November 20, 2015. The case files for the case against ...
Neuroblastoma (aggressive) Jedynak, Andrzej. L Gill Naul (ed.). "Imaging in Ganglioneuroma and Ganglioneuroblastoma". Medscape ... recommendations by the International Neuroblastoma Pathology Committee". Cancer. 86 (2): 349-63. doi:10.1002/(sici)1097-0142( ...
"NEUROBLASTOMA RAS VIRAL ONCOGENE HOMOLOG; NRAS". OMIM. Retrieved 8 April 2014. CS1 maint: discouraged parameter (link) de Vos ...
... or neuroblastoma. Cases of secondary cystathioninuria are not responsive to vitamin B6 administration. Under primary ... "Cystathioninuria in patients with neuroblastoma". Medical and Pediatric Oncology. 12 (2): 81-4. doi:10.1002/mpo.2950120203. ...
Sharp, Susan E.; Trout, Andrew T.; Weiss, Brian D.; Gelfand, Michael J. (January 2016). "MIBG in Neuroblastoma Diagnostic ... 131I-mIBG (metaiodobenzylguanidine) is used for the treatment of phaeochromocytoma and neuroblastoma. 177Lu is bound with a ...
A prior phase I clinical trial for patients with refractory or recurrent neuroblastoma designed to decrease toxicity found safe ... 2014 Jan 21;588(2):288-97 3. Neuroblastoma: developmental biology, cancer genomics and immunotherapy. Cheung NK, Dyer MA. Nat ... GD2 is a disialoganglioside expressed on tumors of neuroectodermal origin, including human neuroblastoma and melanoma, with ... GD2 antibody is currently undergoing a phase II clinical trial in the treatment of previously untreated high risk neuroblastoma ...
Neuroblastoma is a rare form of a malignant cancer which can start in the olfactory nerve, which is responsible for smell ... "Side effects of radiation for olfactory neuroblastoma". Livestrong.com. Retrieved July 3, 2012. Leopold DA, Loehrl TA, Schwob ...
Neuroblastoma screening at one year of age. N Engl J Med 2002 346:1047-1053. Yamamoto K, Hanada R, Kikuchi A, et al. ... Spontaneous regression of localized neuroblastoma detected by mass screening. J Clin Oncol 1998;16:1265-69. Welch HG, Woloshin ... including neuroblastoma, melanoma, and thyroid cancer. In fact, some degree of overdiagnosis in cancer early detection is ...
CS1 maint: discouraged parameter (link) Matthay KK (Jan 2013). "Targeted isotretinoin in neuroblastoma: kinetics, genetics, or ...
Neuroblastoma breakpoint family, member 15, also known as NBPF15, is a protein which in humans is encoded by the NBPF15 gene. ... "Entrez Gene: NBPF16 neuroblastoma breakpoint family, member 16". Retrieved 13 May 2009. "SDSC Biology Workbench". San Diego ...
Neuroblastoma breakpoint family, member 3, also known as NBPF3, is a human gene of the neuroblastoma breakpoint family, which ... "Entrez Gene: NBPF3 neuroblastoma breakpoint family, member 3". "UniProt". Retrieved 14 May 2009. Vandepoele K, Van Roy N, Staes ... It has also been suggested that members of the neuroblastoma breakpoint family are tumor suppressor genes, due to a loss of ... It has also been suggested that there is a connection between the neuroblastoma breakpoint family and oncogenesis. Due to the ...
These include extraspinal ependymoma, ependymoblastoma, neuroblastoma and rhabdomyosarcoma. Smaller SCTs with an external ...
Preclinical studies are investigating lorlatinib for treatment of neuroblastoma. In 2017, Pfizer announced that lorlatinib was ...
A tumor arising from these cells is called neuroblastoma. 2) Chromaffin cells (or pheochromocytes): These cells will migrate to ...
The organization has become the largest single funder of neuroblastoma research at MSKCC. Neuroblastoma is a form of childhood ... Help Fight Childhood Cancer Neuroblastoma Children's Cancer Alliance UK The Neuroblastoma Society "Father of Talia Castellano ... Even with aggressive therapy, stage IV neuroblastoma carries a poor prognosis, with a three-year survival rate of 30-40%. ... In addition to surgery, chemotherapy and radiation, many neuroblastoma patients at MSKCC were treated with a murine (mouse- ...
"Neuroblastoma-derived secretory protein is a novel secreted factor overexpressed in neuroblastoma". Mol. Cancer Ther. 8 (8): ... "Neuroblastoma-derived secretory protein messenger RNA levels correlate with high-risk neuroblastoma". J. Pediatr. Surg. 42 (1 ... mRNA levels were tested in 45 neuroblastoma tumor samples; in 43 of these samples, elevated levels of NDSP were found, as well ... FAM163A, also known as cebelin and neuroblastoma-derived secretory protein (NDSP) is a protein that in humans is encoded by the ...
Chemotherapy for neuroblastoma may be effective, although data is contradictory and unconvincing at this point in time. ... After a diagnosis of OMS is made, an associated neuroblastoma is discovered in half of cases, with median delay of 3 months. ... It affects 2 to 3% of children with neuroblastoma and has been reported to occur with celiac disease and diseases of neurologic ... One study concludes that: "Patients with OMA and neuroblastoma have excellent survival but a high risk of neurologic sequelae. ...
"Containment and growth of neuroblastoma cells on chemically patterned substrates". Journal of Neuroscience Methods. 50 (2): 253 ...
March 2016). "Entrectinib is a potent inhibitor of Trk-driven neuroblastomas in a xenograft mouse model". Cancer Letters. 372 ( ... It has an EU orphan designation for neuroblastoma. FDA approved entrectinib for people with ROS1-positive, metastatic non-small ... entrectinib has orphan drug designation and rare pediatric disease designation for the treatment of neuroblastoma and orphan ... "Entrectinib and other ALK/TRK inhibitors for the treatment of neuroblastoma". Drug Design, Development and Therapy. 12: 3549- ...
MYCN amplification in neuroblastoma has been shown to deplete the abundance of its miRNA regulators, supporting MYCN's role as ... "Multiple mechanisms disrupt the let-7 microRNA family in neuroblastoma". Nature. 535 (7611): 246-51. doi:10.1038/nature18632. ... a master ceRNA regulator in neuroblastoma. Highly Up-regulated in liver cancer (HULC) is one of the most upregulated of all ...
Neuroblastoma is a cancer more frequently affecting children, and despite the relatively low rates of anti-Hu among children ... The cancer-produced Hu proteins are found in nearly all small-cell lung carcinomas, 70 percent of neuroblastomas, and a small ... Meena JP, Seth R, Chakrabarty B, Gulati S, Agrawala S, Naranje P (2016). "Neuroblastoma presenting as opsoclonus-myoclonus: A ... The cancers most associated with anti-Hu encephalitis are neuroblastoma and ganglioneuroblastoma. Opsoclonus-myoclonus syndrome ...
It is also found in Trifolium pratense (red clover). In isolated neuroblastoma cells, clovamide has in vitro neuroprotective ...
... from which it was subcloned was isolated from a bone marrow biopsy taken from a four-year-old female with neuroblastoma. SH- ... this will aid in differention and dendrification of the neuroblastoma. In general the cells are quite robust and will grow in ... "Nuclear RXRα and RXRβ receptors exert distinct and opposite effects on RA-mediated neuroblastoma differentiation". Biochimica ... "Molecular mechanisms involved in the adenosine A1 and A2A receptor-induced neuronal differentiation in neuroblastoma cells and ...
She died of neuroblastoma on August 2, 2014, aged 31. "CLARK Chelsea". Paralympic.org. "Obituary: Chelsea Clark's spirit ...
Juniper extracts induce calcium signalling and apoptosis in neuroblastoma cells. Journal of Pharma and Pharmaeutical Sciences. ... Scrophularia orientalis extract induces calcium signaling and apoptosis in neuroblastoma cells. International Journal of ...
June 2013). "Dependence receptor UNC5D mediates nerve growth factor depletion-induced neuroblastoma regression". The Journal of ... the spontaneous regression characteristic of type IV-S neuroblastoma, neurodegenerative cell death, inhibition of new tumor ... highlighted the role of the dependence receptor UNC5D in the phenomenon of spontaneous regression of type IV-S neuroblastoma. ...
In vitro work with DCA on neuroblastomas (which have fewer recognised mitochondrial abnormalities) showed activity against ... "Dichloroacetate inhibits neuroblastoma growth by specifically acting against malignant undifferentiated cells". International ...
Such screens have been used to identify cancer-essential genes in acute myeloid leukemia and neuroblastoma, and to describe ... January 2018). "CRISPR-Cas9 screen reveals a MYCN-amplified neuroblastoma dependency on EZH2". The Journal of Clinical ...
Neuroblastoma is the most common extracranial solid tumor of infancy. It is an embryonal malignancy of the sympathetic nervous ... Histologic subtypes of neuroblastoma are shown in the image below.. Histologic subtypes of neuroblastoma. Top right panel, ... Histologic subtypes of neuroblastoma. Top right panel, neuroblastoma: A monotonous population of hyperchromatic cells with ... Improved Survival of Children with Neuroblastoma Between 1979 and 2005: A Report of the Italian Neuroblastoma Registry. J Clin ...
The funding supports their work to find new treatments for neuroblastoma. The survival rate for neuroblastoma is currently less ... Previous studies by his research group have shown that NKTs localize to the tumor site in neuroblastoma patients and attack ... The data gained from the study will help researchers determine the mechanisms of treatment resistance in neuroblastoma and ... including some forms of neuroblastoma, are still very hard to treat," said Dr. David Poplack, director of Texas Childrens ...
Treatments and Tools for neuroblastoma. Find neuroblastoma information, treatments for neuroblastoma and neuroblastoma symptoms ... neuroblastoma - MedHelps neuroblastoma Center for Information, Symptoms, Resources, ... I was born with a neuroblastoma tumor located on the right side of my neck. It was removed ... ... My 10month old baby boy has neuroblastoma a lump 9cm in abdomen. How long before the chemo ... ...
Cell Line, Tumor, Exome, Genetic Predisposition to Disease, Genome, Human, Humans, Mutation, Neuroblastoma, Polymorphism, ... Neuroblastoma is a malignancy of the developing sympathetic nervous system that often presents with widespread metastatic ... The relative paucity of recurrent somatic mutations in neuroblastoma challenges current therapeutic strategies that rely on ... To determine the spectrum of somatic mutation in high-risk neuroblastoma, we studied 240 affected individuals (cases) using a ...
Moreover, compared with neuroblastomas without MYCN amplification, MYCN-amplified neuroblastomas expressed higher levels of ... Pharmacological inhibition of EZH2 inhibits neuroblastoma growth in vitro. (A and B) Neuroblastoma cells treated with the EZH2 ... To study neuroblastoma-specific, EZH2-regulated transcriptional programs, we profiled 2 MYCN-amplified neuroblastoma cell lines ... Amplified DNA with limited homology to myc cellular oncogene is shared by human neuroblastoma cell lines and a neuroblastoma ...
Pediatric neuroblastoma in its advanced stage (st. IV) is usually lethal. 70% of the affected children die. 50% of the children ... Therefore a new treatment of neuroblastoma with a different antitumoral mode of action than the traditional cytotoxics is ... Bone Morphogenetic Protein 4 (BMP4) for Treating Advanced Stage Neuroblastoma. 09.07.2013 ...
... human neuroblastoma cell line) whole cell lysate. ab3956 has been validated in western blot. Abcam now offers a 12-month ... Cell line: SK-N-SH (Human neuroblastoma).. Growth media: Minimum essential medium Eagle with 2 mM L-glutamine and Earles BSS ...
The Neuroblastoma Society, About us The Neuroblastoma Society, Befriending Support The Neuroblastoma Society. ... The Neuroblastoma Society is a UK charity that helps families affected by neuroblastoma by funding research and running a ... The charity was set up in 1982 by a group of parents whose children had suffered from or died of neuroblastoma. ...
Neuroblastoma often spreads to other parts of the body before any symptoms are apparent, and 50 to 60% of all neuroblastoma ... Another way to detect neuroblastoma is the meta-iodobenzylguanidine scan, which is taken up by 90 to 95% of all neuroblastomas ... The "International Neuroblastoma Staging System" (INSS) established in 1986 and revised in 1988 stratifies neuroblastoma ... The new INRG risk assignment will classify neuroblastoma at diagnosis based on a new International Neuroblastoma Risk Group ...
Neuroblastoma is a common solid tumor of infancy and childhood. It is uniquely characterized by a distinct natural history and ... Neuroblastoma in the newborn. A study of the Italian Neuroblastoma Registry. Eur J Cancer. 2009;45:3220-7.CrossRefPubMedGoogle ... Neurocytoma or neuroblastoma, a kind of tumor not generally recognized. J Exp Med. 1910;12:556-61.PubMedPubMedCentralCrossRef ... Neonatal neuroblastoma. Arch Dis Child Fetal Neonatal Ed. 1999;81:F134-7.PubMedPubMedCentralCrossRefGoogle Scholar ...
Neuroblastoma is a type of cancer that most often affects children. Explore symptoms, inheritance, genetics of this condition. ... Neuroblastoma is a type of cancer that most often affects children. Neuroblastoma occurs when immature nerve cells called ... When neuroblastoma is associated with somatic mutations, it is called sporadic neuroblastoma. It is thought that somatic ... Most people with neuroblastoma have sporadic neuroblastoma, meaning the condition arose from somatic mutations in the bodys ...
Neuroblastoma Definition Neuroblastoma is a type of cancer that usually originates either in the tissues of the adrenal gland [ ... Neuroblastoma Gale Encyclopedia of Cancer COPYRIGHT 2002 The Gale Group Inc.. Neuroblastoma. Definition. Neuroblastoma is a ... Neuroblastoma Gale Encyclopedia of Medicine, 3rd ed. COPYRIGHT 2006 Thomson Gale. Neuroblastoma. Definition. Neuroblastoma is a ... The causes of neuroblastoma are not precisely known. Current research holds that neuroblastomas develop when cells produced by ...
Neuroblastoma is difficult to detect in the early stages and the disease is often not identified until it is more advanced. In ... High-risk neuroblastomas are more likely to recur after treatment.. Those who survive neuroblastoma may develop certain ... Neuroblastoma is difficult to detect in the early stages and the disease is often not identified until it is more advanced. In ... Study identifies improved treatment option for neuroblastoma. *Causes of disease including infant cancer can be revealed with ...
Treatment of neuroblastoma is aimed at increasing a childs survival, reducing the risk of recurrence and ensuring an as near- ... Chemotherapy for neuroblastoma. A variety of different chemotherapy agents may be used in combination to treat neuroblastoma. ... Surgery for neuroblastoma. Surgical techniques may be employed to confirm a diagnosis and/or to remove a tumor. For smaller ... Treatment of neuroblastoma is aimed at increasing a childs survival, reducing the risk of recurrence and ensuring an as near- ...
... including neuroblastoma, ganglioneuroblastoma, and ganglioneuroma are composed of biologically favorable and biologically ... International consensus for neuroblastoma molecular diagnostics: report from the International Neuroblastoma Risk Group (INRG) ... MYC family-driven neuroblastoma (including large-cell neuroblastoma) defined by augmented expression of MYCN and/or MYC protein ... Recent advances in neuroblastoma. N Engl J Med. 2010;362:2202-11.CrossRefPubMedPubMedCentralGoogle Scholar ...
The signs and symptoms of neuroblastoma vary widely, depending on the size of the tumor, where it is, how far it has spread, ... Neuroblastoma often spreads to bones. A child who can talk may complain of bone pain. The pain may be so bad that the child ... Neuroblastoma. In: Pizzo PA, Poplack DG, eds. Principles and Practice of Pediatric Oncology. 7th ed. Philadelphia Pa: ... Neuroblastomas that press on certain nerves in the chest or neck can sometimes cause other symptoms, such as a drooping eyelid ...
Researchers have studied whether screening infants for neuroblastoma might result in earlier diagnosis and better treatment ... Can Neuroblastoma Be Found Early?. Researchers have studied whether screening infants for neuroblastoma might find these tumors ... One way to screen for neuroblastoma is to test childrens urine for certain substances made by neuroblastoma tumors. (For more ... In rare instances, neuroblastoma is found before birth during an ultrasound, a test that uses sound waves to create an image of ...
A sarcoma which is made up of primitive neural tissue, developing most often in the brain or in the adrenal glands above the kidneys and frequently spre...
... neuroblastoma arises in the adrenal glands, located in the abdominal area near the kidneys, and along the sympathetic nerve ... What is Neuroblastoma? Found only in children, neuroblastoma arises in the adrenal glands, located in the abdominal area near ... Neuroblastoma spreads quickly, and often is discovered only after the disease is widespread. Early stages are curable by ... What are the Treatment Options for Neuroblastoma?. The types of treatment used most often to treat childhood cancer are surgery ...
... radiation and diagnostic care for children with neuroblastoma. Learn how we can provide the latest in care for your child. ... Weiss leads national studies on neuroblastoma therapies and is a leader in the Childrens Oncology Groups (COG) neuroblastoma ... refractory neuroblastoma. Our Neuroblastoma Advanced Therapies Center offers patients and families a multidisciplinary team ... The director of the Neuroblastoma Advanced Therapies Center is Brian Weiss, MD. Weiss is a national leader in the study of ...
Division of Neuroblastoma Genomics. PD Dr. Frank Westermann. Hierarchical cluster analysis with clinical covariates of 702 ... Neuroblastoma is the most common pediatric single-entity solid cancer derived from primitive cells of the peripheral ... Malfunction of the MYCN transcription factor is a key feature of neuroblastoma biology and is involved in almost every aspect ... In addition, we use high-throughput si/shRNA screens to disclose neuroblastoma-specific vulnerabilities associated with ...
... neuroblastoma is the most common type that occurs outside of the brain and skull. Still, only about 700 cases of the tumor are ... What is Pediatric Neuroblastoma?. If a neuroblastoma is diagnosed in a young patient, or a possible tumor is detected in a ... Pediatric Neuroblastoma. Neuroblastoma is the most common pediatric solid tumor occurring outside the brain and it is the most ... How is Pediatric Neuroblastoma diagnosed?. Neurological and physical exams can detect the effects of a neuroblastoma on spinal ...
Neuroblastoma is the most common cancer among infants, suggesting an etiologic role for prenatal factors. In this case-cohort ... Perinatal risk factors for neuroblastoma.. McLaughlin CC1, Baptiste MS, Schymura MJ, Zdeb MS, Nasca PC. ... Generally, more risk factors were identified as associated with neuroblastoma among younger infants relative to older ages, ... study, neuroblastoma cases (n = 529) diagnosed between 1985 and 2001 were identified from the New York State Cancer Registry ...
... is the most common solid tumor, other than brain tumors, in children. The great majority of cases affect young ... Neuroblastoma. Make An Appointment: 410-955-8751. About Neuroblastoma. Neuroblastoma is the most common solid tumor, other than ... No environmental factors are known to cause neuroblastoma, and neuroblastoma rarely runs in families.. Neuroblastoma tends to ... Neuroblastoma Staging. There is an international staging system for neuroblastoma:. Stage 1 - A single tumor that is removed ...
3.1.5. MMP Expression in Human Neuroblastoma. In advanced stages of neuroblastoma, tumor cell secrets MMP favoring degradation ... HGF and c-Met are upregulated in many human cancers including neuroblastoma [84] and highly responsible for neuroblastoma ... Biological effects of TrkA and TrkB expression on neuroblastoma angiogenesis were examined in human neuroblastoma SH-SY5Y cell ... are expressed in high-risk neuroblastoma [45]. The binding of the integrin 𝛼. 𝑣. 𝛽. 3. to its receptor provides a signal that ...
Learn more about pediatric research studies related to neuroblastoma relapse / refractory at Cincinnati Childrens Hospital ... Conditions: Neuroblastoma Relapse - Refractory, Sarcoma Osteosarcoma Relapse, Sarcoma Ewing Relapse, Sarcoma Rhabdomyosarcoma ... To learn more about available neuroblastoma clinical trials, contact us at [email protected] or 513-636-2799. ... Neuroblastoma Relapse - Refractory, Liver Relapse - Refractory, Kidney Relapse - Refractory, Solid Tumors - All Other, Solid ...
... A neuroblastoma is a malignant (cancerous) tumour, which develops in the nerve cells that run in a chain down ... Treatment of Neuroblastoma. Treatment for neuroblastoma depends on the age, size and position of the tumour and whether it has ... Who gets neuroblastoma?. Less than 100 children develop neuroblastoma in the UK each year. It most often affects children under ... Neuroblastoma can present in many different ways, but usually it presents as a lump in the tummy (abdomen). Other symptoms ...
Neuroblastoma can occur anywhere along the peripheral nervous system, so symptoms vary with the location. Learn about the ... Neuroblastoma in the abdomen or pelvis. Neuroblastoma in the abdomen or pelvis can cause:* a lump or swelling in the abdomen ... Symptoms of neuroblastoma. Neuroblastoma may not cause any signs or symptoms in its early stages. Signs and symptoms often ... Neuroblastoma affecting the spinal cord. Neuroblastoma can sometimes affect the spinal cord and cause:* weakness in the legs ...
Neuroblastoma starts in immature nerve cells, developing mostly in infants and young children. Learn about neuroblastoma in our ... What is neuroblastoma?. Neuroblastoma is a type of childhood cancer that starts in immature nerve cells in the sympathetic ... Neuroblastoma most commonly develops in babies and young children. In rare cases, changes to neuroblasts can lead to ... In some cases, changes to neuroblasts can cause neuroblastoma. It can develop anywhere in the sympathetic nervous system, but ...
If your child has been diagnosed with neuroblastoma, were ready to help. Find a doctor or a clinical trial and learn about our ... The Most Experienced Neuroblastoma Program in the Country. Hearing that your child has neuroblastoma can be frightening and ... In addition to the worlds best neuroblastoma therapies, the neuroblastoma program at MSK Kids has services that nurture your ... In fact, neuroblastoma is the cancer most often found in infants. Tumors typically begin in the belly, neck, chest, pelvis, or ...
According to a mutation model, two classes of individuals could acquire neuroblastoma. One (prezygotic) was a rare class that ... Case reports of neuroblastoma revealed that some individuals are genetically predisposed and that this genetic predisposition ... The phenomena of aganglionosis, neuroblastoma in situ, maturation of neuroblastoma to ganglioneuroma, and spontaneous ... Developmental genetics of neuroblastoma J Natl Cancer Inst. 1976 Sep;57(3):675-82. doi: 10.1093/jnci/57.3.675. ...
Samantha was in Stage IV, with a Neuroblastoma Cancer and given a 30% chance of survival. ...
Neuroblastoma: Overview Video Neuroblastoma: Overview Video. This video covers key aspects of neuroblastoma, including the ... New Drug Approved for Neuroblastoma Based on MSKCC Research New Drug Approved for Neuroblastoma Based on MSKCC Research. ... New method for monitoring residual disease after treatment in children with neuroblastoma. (Kobe University) Neuroblastoma is a ... Danyelza Gains Accelerated Approval to Treat Neuroblastoma Neuroblastoma is a solid tumor of childhood that occurs in the ...
Pixdaus.com is an environmental photo-bookmarking and sharing service. All the pictures are uploaded by users and the copyrights belong to the rightholders. You can request a removal using the Copyright flag link below each image if you find an image that shouldnt belong here ...
... The Neuroblastoma Program is dedicated to providing state-of-the-art care for pediatric patients ...
  • Neuroblastoma is the most common extracranial solid tumor of infancy. (medscape.com)
  • I was born with a neuroblastoma tumor located on the right side of my neck. (medhelp.org)
  • Neuroblastoma is a cancerous tumor that develops from nerve tissue in infants and children. (itnonline.com)
  • Neuroblastoma is an aggressive, relapse-prone childhood tumor of the sympathetic nervous system that accounts for 15% of pediatric cancer deaths. (elsevier.com)
  • A distal portion of human chromosome 3p is often deleted in neuroblastoma, this region may contain one or more putative tumor suppressor genes. (elsevier.com)
  • Over-expression of CHL1 induced neurite-like outgrowth and markers of neuronal differentiation in neuroblastoma cells, halted tumor progression, inhibited anchorage-independent colony formation, and suppressed the growth of human tumor xenografts. (elsevier.com)
  • CHL1 is a novel candidate tumor suppressor in neuroblastoma, and its associated pathways may represent a promising target for future therapeutic interventions. (elsevier.com)
  • This CT scan of the upper abdomen shows a large neuroblastoma tumor on a person's right side (lower left side of picture). (diseasefix.com)
  • Neuroblastoma was one of the tumor types on which DCA was considered ineffective as it is composed of cells with few recognized mitochondrial anomalies. (geoscience.net)
  • Neuroblastoma is the most common extracranial solid tumor in infants and children. (tdl.org)
  • In MYCN amplified neuroblastoma, elevated expression of the factor is crucial to maintain tumor stemness and is associated with increased proliferation and aberrant cell cycle progression, as these tumors lack the ability to arrest in G1 in response to irradiation. (omicsdi.org)
  • MYCN down-regulation broadly reverses these oncogenic phenotypes in a variety of neuroblastoma models and recent thereapeutic strategies to indirectly target MYCN production or protein stability have reduced tumor growth in vivo. (omicsdi.org)
  • Background: Neuroblastoma is the third most common solid tumor in children. (elsevier.com)
  • To verify the practical utility of immunohistochemical analysis of bone marrow biopsy specimens in patients with neuroblastoma, we compared the results of routine histologic examination of 68 specimens with the results of immunohistochemical detection of tumor cells using an antibody to neuron-specific enolase (NSE). (umn.edu)
  • Notably, anti-NSE detected small foci of metastatic neuroblastoma in two of 43 biopsy specimens that showed no evidence of metastatic tumor in the initial histologic sections. (umn.edu)
  • Rare NSE-reactive hematopoietic cells were present in approximately a third of the specimens with and those without neuroblastoma and were easily distinguished from metastatic tumor by morphologic examination. (umn.edu)
  • We conclude that this antibody to NSE consistently detects neuroblastoma cells in routinely processed bone marrow specimens, including small foci of tumor cells not evident in initial histologic sections. (umn.edu)
  • Neuroblastomas are the most common extracranial solid childhood malignancies and the third commonest childhood tumors after leukemia and brain malignancies. (radiopaedia.org)
  • Stage 4S neuroblastoma tumors show a characteristic DNA methylation portrait. (uclouvain.be)
  • To this purpose, differential methylation analyses between International Neuroblastoma Staging System (INSS) stage 4S, stage 4 and stage 1/2 were performed, using methyl-CpG-binding domain (MBD) sequencing data of 14 stage 4S, 14 stage 4, and 13 stage 1/2 primary NB tumors (all MYCN non-amplified in order not to confound results). (uclouvain.be)
  • These observations motivate an investigation of MYCN binding in MYCN amplified tumors as it remains fundamentally unclear how elevated levels of the factor occupy the genome and alter transcriptional programs in neuroblastoma. (omicsdi.org)
  • In the study, eight children with relapsed or primary refractory neuroblastoma were imaged with a 68Ga-DOTATATE PET/CT scan. (itnonline.com)
  • In neuroblastoma, amplification of the oncogenic basic helix-loop-helix (bHLH) transcription factor (TF) MYCN is the defining prognosticator of high-risk disease, occurs in one-third of neuroblastoma, and drastically reduces overall survival rates. (omicsdi.org)
  • Here we present the first dynamic chromatin and transcriptional landscape of direct MYCN perturbation in neuroblastoma. (omicsdi.org)
  • These data suggest that MYCN enhancer invasion helps shape transcriptional amplification of the neuroblastoma gene expression program to promote tumorigenesis. (omicsdi.org)
  • One of many down-stream MYCN targets is miR-184, which is either directly or indirectly repressed by this transcription factor, possibly due to its pro-apoptotic effects when ectopically over-expressed in neuroblastoma cells. (rcsi.ie)
  • As an inhibitor of AKT2, miR-184 could be of potential benefit in miRNA mediated therapeutics of MYCN amplified neuroblastoma and other forms of cancer. (rcsi.ie)
  • The patterns of distribution of these cells correlates with the sites of primary neuroblastoma presentation. (medscape.com)
  • Top right panel, neuroblastoma: A monotonous population of hyperchromatic cells with scant cytoplasm. (medscape.com)
  • Here we showed that N-Myc upregulated HDAC5 expression in neuroblastoma cells. (garvan.org.au)
  • Moreover, HDAC5 blocked differentiation and induced proliferation in neuroblastoma cells. (garvan.org.au)
  • A multidisciplinary team from diverse research centres in Madrid, including various hospitals, Universidad Politécnica de Madrid (UPM) and Complutense (UCM), has developed synthetic molecules which are able to specifically attach to neuroblastoma cells, therefore being a new form of neuroblastoma treatment. (healtheuropa.eu)
  • Researchers have synthesised a family of molecules which are able to specifically bind a protein found in the cell membrane of more than 90% of neuroblastoma cells, the norepinephrine transporter (NET). (healtheuropa.eu)
  • This way, the process of recognition is highly selective since the binding only occurs with the suitable "padlock" which is only located in the surface of neuroblastoma cells avoiding thus the healthy cells. (healtheuropa.eu)
  • We analyzed cytogenetically blood cells and bone marrow cells from 20 neuroblastoma patients. (elsevier.com)
  • Our findings demonstrate unambiguously that CHL1 acts as a regulator of proliferation and differentiation of neuroblastoma cells through inhibition of the MAPKs and Akt pathways. (elsevier.com)
  • Neuroblastoma is a type of cancer that occurs in the immature nerve cells of several areas of the body. (diseasefix.com)
  • Neuroblastoma most commonly develops in and around the adrenal glands, which have similar origins to nerve cells and are located on top of the kidneys. (diseasefix.com)
  • Survival and distribution of transplanted human IMR-32 neuroblastoma cells. (muscimol.xyz)
  • Fluorescent latex microspheres were used to label cultured human neuroblastoma IMR-32 cells prior to transplantation. (muscimol.xyz)
  • r\n\r\nGRP/BBS treatment rapidly increased phosphorylation of both Akt and GSK-3ƒÒ in neuroblastoma cells. (tdl.org)
  • When glioblastoma and neuroblastoma cells are mixed, an inhibition of 14 CO 2 evolution from [1- 14 C]-glucose occurs. (elsevier.com)
  • This does not occur when Hela and Glioblastoma cells or Hela and neuroblastoma cells are mixed. (elsevier.com)
  • Materials and Methods: BE-2(c) and IMR-32 neuroblastoma cell lines were treated with varying concentrations of αIR3, rapamycin and temsirolimus alone or in combination and the viable cells were counted. (elsevier.com)
  • Conclusion: The combination of rapamycin or temsirolimus with αIR3 blocks the IGF1R signaling pathway and has an antiproliferative effect on neuroblastoma cells warranting further investigations using inhibitors of IGF1R signaling as novel combination therapy for neuroblastoma. (elsevier.com)
  • A commercially available polyclonal antibody to this enolase isoform consistently reacted with the neoplastic cells in biopsy specimens with histologic features diagnostic of (24 specimens) or suspicious for (one specimen) metastatic neuroblastoma. (umn.edu)
  • Side effects of neuroblastoma cancer treatable with surgery? (medhelp.org)
  • Neuroblastoma is a rare type of cancer that mostly affects babies and young children. (diseasefix.com)
  • This mode of treatment has great potential for children whose treatment options are limited, as neuroblastoma often becomes resistant to chemotherapy and success is limited by poor bone marrow reserve," said Bomanji. (itnonline.com)
  • Patients with low-risk and intermediate-risk neuroblastoma have excellent prognosis and outcome. (medscape.com)
  • Deletion of the short arm of chromosome 1 is the most common chromosomal abnormality present in neuroblastoma and confers a poor prognosis. (medscape.com)
  • Moats-Staats, Billie M. / IGF-I receptor inhibition combined with rapamycin or temsirolimus inhibits neuroblastoma cell growth . (elsevier.com)
  • MicroRNA-184 inhibits neuroblastoma cell survival through targeting th" by Niamh H. Foley, Isabella Bray et al. (rcsi.ie)
  • MicroRNA-184 inhibits neuroblastoma cell survival through targeting the serine/threonine kinase AKT2. (rcsi.ie)
  • The pro-apoptotic effects of miR-184 ectopic over-expression in neuroblastoma cell lines is reproduced by siRNA inhibition of AKT2, while a positive effect on cell numbers similar to that obtained by the knock-down of endogenous miR-184 can be achieved by ectopic up-regulation of AKT2. (rcsi.ie)
  • Neuroblastoma, however, is composed of different cell types in terms of metabolism, phenotype and malignant potential. (geoscience.net)
  • The differences in outcome for patients with neuroblastoma are striking. (medscape.com)
  • Over the last 2 decades, many chromosomal and molecular abnormalities have been identified in patients with neuroblastoma. (medscape.com)
  • The treatment was feasible, practical and well-tolerated in the small group of patients with high-risk neuroblastoma. (itnonline.com)
  • All neuroblastoma patients showed a higher increase of aberrations after aphidicolin treatment as compared to that found in normal controls. (elsevier.com)
  • We found that low CHL1 expression predicted poor outcome in neuroblastoma patients. (elsevier.com)
  • For this project, two neuroblastoma cell lines UKF-NB-3 and UKF-NB-6, derived from late-stage high-risk neuroblastoma patients, have been adapted to cisplatin in order to investigate the emergence of resistance. (kent.ac.uk)
  • This study looks at how well response and biology-based risk factor-guided therapy works in treating younger patients with non-high risk neuroblastoma. (studypages.com)
  • Response and biology-based risk factor-guided therapy may be effective in treating patients with non-high risk neuroblastoma and may help to avoid some of the risks and side effects related to standard treatment. (studypages.com)
  • Signs and symptoms of neuroblastoma may include fatigue, loss of appetite, and fever. (diseasefix.com)
  • The genetic landscape of high-risk neuroblastoma. (broadinstitute.org)
  • To determine the spectrum of somatic mutation in high-risk neuroblastoma, we studied 240 affected individuals (cases) using a combination of whole-exome, genome and transcriptome sequencing as part of the Therapeutically Applicable Research to Generate Effective Treatments (TARGET) initiative. (broadinstitute.org)
  • The study tested the principle that combined positron emission tomography and X-ray computed tomography (PET/CT) may be used to select children with primary refractory or relapsed high-risk neuroblastoma for treatment with a molecular radiotherapy known as 177Lu-DOTATATE. (itnonline.com)
  • Taken together, our data identify HDAC5 as a novel co-factor in N-Myc oncogenesis, and provide the evidence for the potential application of HDAC5 inhibitors in the therapy of N-Myc-induced neuroblastoma and potentially other c-Myc-induced malignancies. (garvan.org.au)
  • The relative paucity of recurrent somatic mutations in neuroblastoma challenges current therapeutic strategies that rely on frequently altered oncogenic drivers. (broadinstitute.org)
  • Since some neuroblastomas express somatostatin receptors, we felt this approach could be beneficial to children as well," said Jamshed B. Bomanji, MBBS, PhD, FRCR, FRCP, one of the authors of the study "177Lu-DOTATATE Molecular Radiotherapy for Childhood Neuroblastoma. (itnonline.com)
  • Detection of metastatic neuroblastoma in bone marrow biopsy specimens with an antibody to neuron-specific enolase. (umn.edu)
  • is an oncogene that is overexpressed in approximately one quarter of cases of neuroblastoma via the amplification of the distal arm of chromosome 2. (medscape.com)
  • The pathogenesis of neuroblastoma has been related to expression of the type 1 insulin-like growth factor receptor (IGF1R) and to transcription factor MYC-N amplification. (elsevier.com)
  • The HT4 cell line was derived from infection of a mouse neuroblastoma cell line with a retrovirus that encoded the temperature-sensitive (ts) mutant of SV40 large T antigen. (elsevier.com)
  • [eng] Stage 4S neuroblastoma (NB) is a special type of NB found in infants with metastases at diagnosis and is associated with an excellent outcome due to its remarkable capacity to undergo spontaneous regression. (uclouvain.be)
  • According to researchers, newly developed synthetic molecules that deliver drugs to an aggressive tumour in children could be an improved version of neuroblastoma treatment. (healtheuropa.eu)
  • The researchers are involved in the development of specific molecular scaffolds which can deliver drugs and diagnostic agents to the neuroblastoma, an aggressive tumour in children. (healtheuropa.eu)
  • The neuroblastoma is a very aggressive paediatric tumour with a complex diagnosis when detected in the metastatic state. (healtheuropa.eu)
  • In order to determine the in vivo significance of GRP/GRPR, the effects of BBS treatment in nude mice with human neuroblastoma xenografts were assessed. (tdl.org)
  • The image shows stage IV-S neuroblastoma with multiple cutaneous metastases presenting as mobile blue-gray nodules. (diseasefix.com)
  • The long-term survival rate for neuroblastoma is below 40 percent. (itnonline.com)
  • Here we have used two inducible cell models to analyze the impact of CHL1 on neuroblastoma biology. (elsevier.com)
  • Activation of the phosphatidylinositol-3-kinase (PI3K)/Akt pathway, a crucial regulator of cell survival, is associated with poor outcome in neuroblastomas and our lab¡¦s previous work has shown that GRPR regulates the expression of PI3K/Akt pathway components. (tdl.org)
  • These findings identify PI3K/Akt as an important signaling pathway for GRP-mediated neuroblastoma cell growth. (tdl.org)
  • We demonstrate that the knock-down of endogenous miR-184 has the opposite effect of ectopic up-regulation, leading to enhanced neuroblastoma cell numbers. (rcsi.ie)
  • This therapeutic option was found to be a viable option for children with neuroblastomas. (itnonline.com)