Mycobacterium smegmatis
Mycobacterium
Mycobacterium tuberculosis
Mycobacterium bovis
Mycobacterium avium
Nontuberculous Mycobacteria
So-called atypical species of the genus MYCOBACTERIUM that do not cause tuberculosis. They are also called tuberculoid bacilli, i.e.: M. buruli, M. chelonae, M. duvalii, M. flavescens, M. fortuitum, M. gilvum, M. gordonae, M. intracellulare (see MYCOBACTERIUM AVIUM COMPLEX;), M. kansasii, M. marinum, M. obuense, M. scrofulaceum, M. szulgai, M. terrae, M. ulcerans, M. xenopi.
Mycobacteriophages
Mycobacterium Infections, Nontuberculous
Infections with nontuberculous mycobacteria (atypical mycobacteria): M. kansasii, M. marinum, M. scrofulaceum, M. flavescens, M. gordonae, M. obuense, M. gilvum, M. duvali, M. szulgai, M. intracellulare (see MYCOBACTERIUM AVIUM COMPLEX;), M. xenopi (littorale), M. ulcerans, M. buruli, M. terrae, M. fortuitum (minetti, giae), M. chelonae.
Mycobacterium leprae
Mycobacterium fortuitum
Mycobacterium avium Complex
A complex that includes several strains of M. avium. M. intracellulare is not easily distinguished from M. avium and therefore is included in the complex. These organisms are most frequently found in pulmonary secretions from persons with a tuberculous-like mycobacteriosis. Strains of this complex have also been associated with childhood lymphadenitis and AIDS; M. avium alone causes tuberculosis in a variety of birds and other animals, including pigs.
Mycobacterium chelonae
Antitubercular Agents
Drugs used in the treatment of tuberculosis. They are divided into two main classes: "first-line" agents, those with the greatest efficacy and acceptable degrees of toxicity used successfully in the great majority of cases; and "second-line" drugs used in drug-resistant cases or those in which some other patient-related condition has compromised the effectiveness of primary therapy.
Ethambutol
An antitubercular agent that inhibits the transfer of mycolic acids into the cell wall of the tubercle bacillus. It may also inhibit the synthesis of spermidine in mycobacteria. The action is usually bactericidal, and the drug can penetrate human cell membranes to exert its lethal effect. (From Smith and Reynard, Textbook of Pharmacology, 1992, p863)
Mycobacterium marinum
Tuberculosis
Mycobacterium kansasii
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Mycobacterium avium subsp. paratuberculosis
Isoniazid
Galactans
Gene Expression Regulation, Bacterial
Mycobacterium avium-intracellulare Infection
Viomycin
Mycobacterium ulcerans
Cell Wall
Glycopeptides
Rifampin
A semisynthetic antibiotic produced from Streptomyces mediterranei. It has a broad antibacterial spectrum, including activity against several forms of Mycobacterium. In susceptible organisms it inhibits DNA-dependent RNA polymerase activity by forming a stable complex with the enzyme. It thus suppresses the initiation of RNA synthesis. Rifampin is bactericidal, and acts on both intracellular and extracellular organisms. (From Gilman et al., Goodman and Gilman's The Pharmacological Basis of Therapeutics, 9th ed, p1160)
Amino Acid Sequence
Microbial Sensitivity Tests
Glycolipids
Base Sequence
Alanine Racemase
Inositol
An isomer of glucose that has traditionally been considered to be a B vitamin although it has an uncertain status as a vitamin and a deficiency syndrome has not been identified in man. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1379) Inositol phospholipids are important in signal transduction.
Cord Factors
Drug Resistance, Bacterial
Mutation
Culture Media
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Polysorbates
Microbial Viability
Genetic Complementation Test
DNA Transposable Elements
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
Rhamnose
Drug Resistance, Microbial
Methylmannosides
Cloning, Molecular
Gene Deletion
RNA, Bacterial
Streptomycin
Sequence Analysis, DNA
Mycobacterium scrofulaceum
Antibiotics, Antitubercular
BCG Vaccine
Chromatography, Thin Layer
Macrophages
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Mutagenesis, Insertional
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
Species Specificity
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Mycobacteriaceae
Mycobacterium xenopi
Paratuberculosis
Leprosy
Porins
Porins are protein molecules that were originally found in the outer membrane of GRAM-NEGATIVE BACTERIA and that form multi-meric channels for the passive DIFFUSION of WATER; IONS; or other small molecules. Porins are present in bacterial CELL WALLS, as well as in plant, fungal, mammalian and other vertebrate CELL MEMBRANES and MITOCHONDRIAL MEMBRANES.
Sequence Homology, Amino Acid
Mannosides
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Oxazoles
Sequence Alignment
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Dihydrostreptomycin Sulfate
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Genes, Essential
Transformation, Bacterial
Tuberculosis, Bovine
Leprostatic Agents
Anti-Infective Agents
Plasmids
Mannosyltransferases
Alanine Dehydrogenase
An NAD-dependent enzyme that catalyzes the reversible DEAMINATION of L-ALANINE to PYRUVATE and AMMONIA. The enzyme is needed for growth when ALANINE is the sole CARBON or NITROGEN source. It may also play a role in CELL WALL synthesis because L-ALANINE is an important constituent of the PEPTIDOGLYCAN layer.
Nicotinamidase
Mycobacterium haemophilum
Colony Count, Microbial
Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.
Open Reading Frames
Acyltransferases
RNA, Ribosomal, 16S
Operon
DNA Gyrase
A bacterial DNA topoisomerase II that catalyzes ATP-dependent breakage of both strands of DNA, passage of the unbroken strands through the breaks, and rejoining of the broken strands. Gyrase binds to DNA as a heterotetramer consisting of two A and two B subunits. In the presence of ATP, gyrase is able to convert the relaxed circular DNA duplex into a superhelix. In the absence of ATP, supercoiled DNA is relaxed by DNA gyrase.
Substrate Specificity
Kanamycin Resistance
Sputum
Promoter Regions, Genetic
Glycine Dehydrogenase
Dianisidine
Phagosomes
Mannose
Gene Knockout Techniques
Armadillos
Siderophores
Bacterial Typing Techniques
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.
Fatty Acid Synthases
Tuberculosis, Multidrug-Resistant
Tuberculosis resistant to chemotherapy with two or more ANTITUBERCULAR AGENTS, including at least ISONIAZID and RIFAMPICIN. The problem of resistance is particularly troublesome in tuberculous OPPORTUNISTIC INFECTIONS associated with HIV INFECTIONS. It requires the use of second line drugs which are more toxic than the first line regimens. TB with isolates that have developed further resistance to at least three of the six classes of second line drugs is defined as EXTENSIVELY DRUG-RESISTANT TUBERCULOSIS.
Virulence
RecA-Mediated gene conversion and aminoglycoside resistance in strains heterozygous for rRNA. (1/838)
Clinical resistance to aminoglycosides in general is due to enzymatic drug modification. Mutational alterations of the small ribosomal subunit rRNA have recently been found to mediate acquired resistance in bacterial pathogens in vivo. In this study we investigated the effect of 16S rRNA heterozygosity (wild-type [wt] and mutant [mut] operons at position 1408 [1408wt/1408mut]) on aminoglycoside resistance. Using an integrative vector, we introduced a single copy of a mutated rRNA operon (1408 A-->G) into Mycobacterium smegmatis, which carries two chromosomal wild-type rRNA operons; the resultant transformants exhibited an aminoglycoside-sensitive phenotype. In contrast, introduction of the mutated rRNA operon into an M. smegmatis rrnB knockout strain carrying a single functional chromosomal wild-type rRNA operon resulted in aminoglycoside-resistant transformants. Subsequent analysis by DNA sequencing and RNase protection assays unexpectedly demonstrated a homozygous mutant genotype, rRNAmut/rRNAmut, in the resistant transformants. To investigate whether RecA-mediated gene conversion was responsible for the aminoglycoside-resistant phenotype in the rRNAwt/rRNAmut strains, recA mutant strains were generated by allelic exchange techniques. Transformation of the recA rrnB M. smegmatis mutant strains with an integrative vector expressing a mutated rRNA operon (Escherichia coli position 1408 A-->G) resulted in transformants with an aminoglycoside-sensitive phenotype. Subsequent analysis showed stable heterozygosity at 16S rRNA position 1408 with a single wild-type allele and a single resistant allele. These results demonstrate that rRNA-mediated mutational resistance to aminoglycosides is recessive. (+info)Genetic evidence that InhA of Mycobacterium smegmatis is a target for triclosan. (2/838)
Three Mycobacterium smegmatis mutants selected for resistance to triclosan each had a different mutation in InhA, an enoyl reductase involved in fatty acid synthesis. Two expressed some isoniazid resistance. A mutation originally selected on isoniazid also mediated triclosan resistance, as did the wild-type inhA gene on a multicopy plasmid. Replacement of the mutant chromosomal inhA genes with wild-type inhA eliminated resistance. These results suggest that M. smegmatis InhA, like its Escherichia coli homolog FabI, is a target for triclosan. (+info)Role of acid pH and deficient efflux of pyrazinoic acid in unique susceptibility of Mycobacterium tuberculosis to pyrazinamide. (3/838)
Pyrazinamide (PZA) is an important antituberculosis drug. Unlike most antibacterial agents, PZA, despite its remarkable in vivo activity, has no activity against Mycobacterium tuberculosis in vitro except at an acidic pH. M. tuberculosis is uniquely susceptible to PZA, but other mycobacteria as well as nonmycobacteria are intrinsically resistant. The role of acidic pH in PZA action and the basis for the unique PZA susceptibility of M. tuberculosis are unknown. We found that in M. tuberculosis, acidic pH enhanced the intracellular accumulation of pyrazinoic acid (POA), the active derivative of PZA, after conversion of PZA by pyrazinamidase. In contrast, at neutral or alkaline pH, POA was mainly found outside M. tuberculosis cells. PZA-resistant M. tuberculosis complex organisms did not convert PZA into POA. Unlike M. tuberculosis, intrinsically PZA-resistant M. smegmatis converted PZA into POA, but it did not accumulate POA even at an acidic pH, due to a very active POA efflux mechanism. We propose that a deficient POA efflux mechanism underlies the unique susceptibility of M. tuberculosis to PZA and that the natural PZA resistance of M. smegmatis is due to a highly active efflux pump. These findings may have implications with regard to the design of new antimycobacterial drugs. (+info)A mutant of Mycobacterium smegmatis defective in the biosynthesis of mycolic acids accumulates meromycolates. (4/838)
Mycolic acids are a major constituent of the mycobacterial cell wall, and they form an effective permeability barrier to protect mycobacteria from antimicrobial agents. Although the chemical structures of mycolic acids are well established, little is known on their biosynthesis. We have isolated a mycolate-deficient mutant strain of Mycobacterium smegmatis mc2-155 by chemical mutagenesis followed by screening for increased sensitivity to novobiocin. This mutant also was hypersensitive to other hydrophobic compounds such as crystal violet, rifampicin, and erythromycin. Entry of hydrophobic probes into mutant cells occurred much more rapidly than that into the wild-type cells. HPLC and TLC analysis of fatty acid composition after saponification showed that the mutant failed to synthesize full-length mycolic acids. Instead, it accumulated a series of long-chain fatty acids, which were not detected in the wild-type strain. Analysis by 1H NMR, electrospray and electron impact mass spectroscopy, and permanganate cleavage of double bonds showed that these compounds corresponded to the incomplete meromycolate chain of mycolic acids, except for the presence of a beta-hydroxyl group. This direct identification of meromycolates as precursors of mycolic acids provides a strong support for the previously proposed pathway for mycolic acid biosynthesis involving the separate synthesis of meromycolate chain and the alpha-branch of mycolic acids, followed by the joining of these two branches. (+info)Integron-mediated rifampin resistance in Pseudomonas aeruginosa. (5/838)
A new rifampin resistance gene, arr-2, has been found in Pseudomonas aeruginosa. The ARR-2 protein shows 54% amino acid identity to the rifampin ADP-ribosylating transferase encoded by the arr gene from Mycobacterium smegmatis. This arr-2 gene is located on a gene cassette within a class I integron. (+info)Apoptosis of Mycobacterium avium-infected macrophages is mediated by both tumour necrosis factor (TNF) and Fas, and involves the activation of caspases. (6/838)
Mycobacterium avium causes disseminated infection in AIDS patients and several forms of infection in immunocompetent hosts. Recent studies have shown that M. avium infection of macrophages in vitro leads to apoptosis of significant numbers of infected cells. Several strains of M. avium used to infect human macrophages for 5 days (multiplicity of infection of 10) triggered 28-46% higher levels of apoptosis than observed with uninfected macrophages at the same time points. Mycobacterium avium strains unable to replicate intracellularly (rep-) resulted in a 15% rate of apoptosis, while M. smegmatis-infected monolayers showed the same percentage of apoptotic cells as the uninfected macrophage control. The presence of anti-TNF-alpha antibody reduced apoptosis to 17% and the presence of anti-Fas antibody reduced apoptosis to 10%. When both antibodies were used together, the apoptosis level was 5% above the control. Treatment with TGF-beta also reduced the number of apoptotic cells in infected monolayers. If intracellular growth was inhibited, apoptosis of macrophages decreased significantly. It was also shown that apoptosis was associated with IL-1 beta-converting enzyme (ICE) activation and was significantly reduced by a caspase inhibitor. Gaining understanding of the mechanisms of M. avium-associated apoptosis of macrophages will provide important insight into M. avium pathogenesis. (+info)Enhanced gene replacement in mycobacteria. (7/838)
Allelic replacement will be a vital tool for understanding gene function in mycobacteria. Disruption of the chromosomal hisD gene of Mycobacterium smegmatis by standard gene replacement methods was surprisingly difficult, with most products being caused by illegitimate recombination (IR) events. A recombination assay was therefore developed and used to optimize conditions for homologous recombination (HR) in M. smegmatis. Treatment of competent cells with UV, hydrogen peroxide or mitomycin C did not improve the frequency of HR; however, treatment of the DNA with alkali or UV enhanced recombination frequency, while boiling did not. Applying these observations to allele replacement, UV and alkali treatment of transforming DNA increased HR events with pyrF and hisD, while the level of IR was unchanged. The introduction of ss phagemid DNA improved the level of HR and abolished IR. In Mycobacterium intracellulare the use of alkali-denatured DNA increased the numbers of recombinants obtained with an inactivated 19Ag gene, while in Mycobacterium tuberculosis, inactivation of a putative haemolysin gene, tlyA, was achieved using both UV-irradiated DNA and ss phagemid DNA. Significantly, IR, which has been reported to be a problem in this species, was not observed. Thus, four genes in three species were successfully knocked-out using non-replicating DNA pretreated with alkali, UV or in an ss form. The use of these methods to enhance HR will greatly facilitate experiments to inactivate other genes in these important species. (+info)Enhancing the immunotherapeutic potential of mycobacteria by transfection with tumour necrosis factor-alpha. (8/838)
In an attempt to enhance the anti-tumour properties of mycobacteria we have developed recombinant forms of Mycobacterium smegmatis which express and secrete biologically active human tumour necrosis factor-alpha (TNF-alpha). This was achieved by transfecting M. smegmatis using shuttle plasmids incorporating the cDNA sequence for the human TNF-alpha mature peptide. In vitro experiments on a panel of human bladder tumour cell lines (EJ18, MGH-U1, RT4, RT112) indicate that our genetically modified mycobacteria are more effective than wild-type at inducing or up-regulating the expression of intracellular adhesion molecule-1 and the secretion of an array of proinflammatory cytokines [interleukin-1 (IL-1), IL-6, IL-8, granulocyte-macrophage colony-stimulating factor]. We have also demonstrated increased adhesion molecule and cytokine expression in response to mycobacteria transfected with vector containing no gene insert. However, this was not as pronounced as that observed following tumour cell stimulation by the TNF-alpha-transfected strain. In contrast, in three out of four tumour cell lines all M. smegmatis strains were found to down-regulate the secretion of the anti-inflammatory cytokine transforming growth factor-beta1. Our studies have also confirmed that M. smegmatis is a powerful inhibitor of bladder tumour cell growth and revealed that its antiproliferative potency is enhanced by transfecting with human TNF-alpha and, to a lesser extent, with vector alone. All M. smegmatis strains were effective in the activation of peripheral blood leucocyte cultures. However, no differences were observed in the ability of the TNF-alpha-transfected, mock-transfected and wild-type mycobacteria to induce tumour cell killing activity. These results suggest that the immunomodulatory effects of M. smegmatis can be enhanced by transfection with vectors which allow the secretion of human TNF-alpha, thus increasing mycobacterial immunotherapeutic potential. (+info)
ASMscience | Developing and Mature Biofilms Produced by Mycobacterium smegmatis
Mycobacterium smegmatis (Trevisan 1889) Lehmann & Neumann 1899 articles
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Encyclopedia of Life
Mycobacterium smegmatis - Redorbit
Mycobacterium smegmatis LfrA protein
Summary Report | CureHunter
Characterisation of VapC in Mycobacterium smegmatis
Interaction of Erp Protein of Mycobacterium tuberculosis with Rv2212 Enhances Intracellular Survival of Mycobacterium smegmatis...
Desiccation - Wikipedia
Pii: s0966-842x(99)01589-9
Lipoarabinomannan binding to lactosylceramide in lipid rafts is essential for the phagocytosis of mycobacteria by human...
The Non-Pathogenic Mycobacteria M. Smegmatis and M. Fortuitum Induce Rapid Host Cell Apoptosis Via a Caspase-3 and TNF...
Ion homeostasis in Mycobacterium smegmatis
MSMEG 6441 (Mycobacterium smegmatis str. MC2 155) | Gene Target - PubChem
NAVER Academic | Effect of isoniazid on glutathione biosynthesis and degradation inMycobacterium smegmatis
Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape | eLife
AID 562238 - Antimycobacterial activity against Mycobacterium smegmatis assessed as growth inhibition in mid-logarithmic phase...
Molecular Mechanism of in Vitro oligomerization of Dps from Mycobacterium smegmatis:mutations of the residues identified by...
British Library EThOS: Comparing the metabolism and metabolic capabilities of Mycobacterium smegmatis vs Mycobacterium bovis...
Browse by Author - [email protected]
MSMEG 4202 - Uncharacterized protein - Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) - MSMEG 4202 gene & protein
A temperature-sensitive Mycobacterium smegmatis glgE mutation leads to a loss of GlgE enzyme activity and thermostability and...
A temperature-sensitive Mycobacterium smegmatis glgE mutation leads to a loss of GlgE enzyme activity and thermostability and...
Structural biology of mycobacterial proteins: The Bangalore effort - [email protected]
RCSB PDB - 4V12: Crystal structure of the MSMEG 6754 dehydratase from Mycobacterium smegmatis
katG2 - Catalase-peroxidase 2 - Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) - katG2 gene & protein
Spatially distinct and metabolically active membrane domain in mycobac by Jennifer M. Hayashi, John D. Leszyk et al.
The Transport of Iron from Ferriexochelin by Mycobacterium smegmatis | Biochemical Society Transactions
Paralogous cAMP receptor proteins in Mycobacterium smegmatis show biochemical and functional divergence. | Sigma-Aldrich
2012 August | microrna inhibitor
SMART: Secondary literature for UTRA domain
Most recent papers with the keyword Cell cycle tuberculosis | Read by QxMD
Ghosh, Pallavi - University at Albany-SUNY
Fine-tuning of Substrate Affinity Leads to Alternative Roles of Mycoba by , Brianne E. Lewis et al.
A0R025 | SWISS-MODEL Repository
archive-edu.com: ferrum.edu - Franklin County Ninth-graders learn teamwork
4rs3 - Summary - PDBj Mine PDB Explorer
Koenzim F420 - Википедија, слободна енциклопедија
The Actinobacteriophage Database | Phage PhrodoBaggins
Identification of three cytotoxic early proteins of mycobacteriophage L5 leading to growth inhibition in Mycobacterium...
Mycobacterium smegmatis L-alanine dehydrogenase (Ald) is required for proficient utilization of alanine as a sole nitrogen...
Frontiers | Separation of Mycobacterium smegmatis From a Mixed Culture Using the Cell Wall Binding Domain of D29...
A Bioinformatics Approach to Revealing the Genetic Basis for Host Range Specificity by Hayley A. Norian
TB vaccine candidate shows early promise
Positional isotope exchange analysis of the Mycobacterium smegmatis cysteine ligase (MshC)<...
Regulation of ornithine decarboxylase from Mycobacterium smegmatis - Publications of the IAS Fellows
Mycobacterium smegmatis (Trevisan) Lehmann and Neumann ATCC ® 607&
Mycobacterium smegmatis (Trevisan) Lehmann and Neumann ATCC ® 607&
Mycobacteriophage L5 gp71 protein
Summary Report | CureHunter
Mycobacterium tuberculosis Lipoprotein MPT83 Induces Apoptosis of Infected Macrophages by Activating the TLR2/p38/COX-2...
PLOS ONE: Expanding the Diversity of Mycobacteriophages: Insights into Genome Architecture and Evolution
The role of pknG in mycobacterial physiology, including M. tuberculosis :: University of Southern California Dissertations and...
Overexpression of inhA, but not kasA, confers resistance to isoniazid and ethionamide in Mycobacterium smegmatis, M. bovis BCG...
OPUS 4 | Thiol-redox proteomics of Mycobacterium smegmatis in response to ROS, RNS and antibiotics
par genes in Mycobacterium bovis and Mycobacterium smegmatisare arranged in an operon transcribed from SigGC promoters | BMC...
ESCRT factors restrict mycobacterial growth.
Aminoglycoside 2-N-acetyltransferase genes are universally present in mycobacteria: characterization of the aac(2)-Ic gene...
The Times Microbial: RecQs Role in Illegitimate Recombination
A0R5E1 | SWISS-MODEL Repository
HHMI at William & Mary - Travel Awards 2007-2010
Malate oxidase
Irradiation of cytoplasm membranes of Mycobacterium smegmatis with ultraviolet light (360 nm) for 10 minutes resulted in about ... "Respiratory chains of Mycobacterium smegmatis". Indian Journal of Biochemistry & Biophysics. 12 (3): 255-259. ISSN 0301-1208. ... "Variations in the pathways of malate oxidation and phosphorylation in different species of Mycobacteria". Biochimica et ...
Mycobacteriophage
While originally isolated from the bacterial species Mycobacterium smegmatis and Mycobacterium tuberculosis, the causative ... A bacteriophage found to infect Mycobacterium smegmatis in 1947 was the first documented example of a mycobacteriophage. It was ... Thousands of mycobacteriophage have been isolated using a single host strain, Mycobacterium smegmatis mc2155, over 1400 of ... Gardner, Grace M.; Weiser, Russell S. (1947). "A Bacteriophage for Mycobacterium smegmatis". Proceedings of the Society for ...
T. A. Venkitasubramanian
214-. ISBN 978-3-540-36488-7. Hugh J O'Neill, Leon D. Gershbein (1976). "Fatty Acids of Mycobacterium Smegmatis Lipids" (PDF). ... "Purification and properties of aspartate transcarbamylase from Mycobacterium smegmatis". Biochim Biophys Acta. 953 (1): 106-113 ... 34 (1): 1-4. doi:10.1016/0378-4274(86)90138-4. PMID 3097877.CS1 maint: multiple names: authors list (link) Mycobacterium ... of Delhi people India portal Biology portal His research findings have been used by others in their researches on mycobacterium ...
Hypothiocyanite
Mycobacterium smegmatis • Mycobacterium abscessus • Neisseria species • Pseudomonas aeruginosa • Pseudomonas pyocyanea • ...
Giles (bacteriophage)
Giles is a bacteriophage that infects Mycobacterium smegmatis bacteria. The genome of this phage is very different from that of ...
Palmitoyl-CoA hydrolase
Yabusaki KK; Ballou CE (1981). Long-chain fatty acyl-CoA thioesterases from Mycobacterium smegmatis. Methods in Enzymology. 71 ...
Beta-mannosylphosphodecaprenol-mannooligosaccharide 6-mannosyltransferase
Yokoyama K, Ballou CE (December 1989). "Synthesis of alpha 1----6-mannooligosaccharides in Mycobacterium smegmatis. Function of ... This enzyme is involved in the formation of mannooligosaccharides in the membrane of Mycobacterium smegmatis. ...
Cofactor engineering
"Metabolic engineering of cofactor F420 production in Mycobacterium smegmatis". PLOS One. 5 (12): e15803. doi:10.1371/journal. ...
Glutamate racemase
Sengupta S, Nagaraja V (February 2008). "Inhibition of DNA gyrase activity by Mycobacterium smegmatis MurI". FEMS Microbiol. ... Sengupta S, Shah M, Nagaraja V (2006). "Glutamate racemase from Mycobacterium tuberculosis inhibits DNA gyrase by affecting its ... Sengupta S, Ghosh S, Nagaraja V (September 2008). "Moonlighting function of glutamate racemase from Mycobacterium tuberculosis ...
Trans,polycis-decaprenyl diphosphate synthase
"Polyprenyl phosphate biosynthesis in Mycobacterium tuberculosis and Mycobacterium smegmatis". Journal of Bacteriology. 182 (20 ... Kaur D, Brennan PJ, Crick DC (November 2004). "Decaprenyl diphosphate synthesis in Mycobacterium tuberculosis". Journal of ...
Dieter Koch-Weser
Glucose Metabolism by Mycobacterium Smegmatis: Evidence for the Pentose Cycle. American Review of Respiratory Diseases. Vol. 86 ... Koch-Weser D. Book Review of Rifampin in the Treatment of Drug-Resistant Mycobacterium tuberculosis Infections" by Vall-Spinosa ...
Manjula Reddy
She uses the model organisms Mycobacterium smegmatis and Escherechia coli. She was part of the team of scientists who patented ...
Trehalase
The trehalase enzyme of Mycobacterium smegmatis is a membrane bound protein. Periplasmic trehalase of Escherichia coli K12 is ...
Decaprenylphospho-beta-D-ribofuranose 2-oxidase
FADH2 This enzyme from the bacterium Mycobacterium smegmatis participates in epimerization of trans,octacis-decaprenylphospho- ... "Analogous mechanisms of resistance to benzothiazinones and dinitrobenzamides in Mycobacterium smegmatis". PLOS ONE. 6 (11): ...
Ribose isomerase
... and properties of D-ribose isomerase from Mycobacterium smegmatis". J. Biol. Chem. 250 (20): 8085-7. PMID 240851. Biology ...
Ashish Arora
"Structural characterization of peptidyl-tRNA hydrolase from Mycobacterium smegmatis by NMR spectroscopy". Biochimica et ...
Penicillin-binding proteins
"Purification and partial characterization of a penicillin-binding protein from Mycobacterium smegmatis". Journal of ...
Lipoarabinomannan
Gilleron M, Himoudi N, Adam O, Constant P, Venisse A, Rivière M, Puzo G (January 1997). "Mycobacterium smegmatis ... purified from Mycobacterium chelonae and Mycobacterium kansasii induce TNF-alpha and IL-8 secretion by a CD14-toll-like ... These types of LAMs are most commonly found in more pathogenic Mycobacterium species such as M. tuberculosis, M. leprae, and M ... The inactivation of macrophages allows for the dissemination of mycobacteria to other parts of the body. The destruction of ...
Methylisocitrate lyase
"Role of the methylcitrate cycle in propionate metabolism and detoxification in Mycobacterium smegmatis". Microbiology. 153 (Pt ... In fact, in some bacteria such as Mycobacterium tuberculosis, isocitrate lyase actually plays the role of methylisocitrate ... "Dual role of isocitrate lyase 1 in the glyoxylate and methylcitrate cycles in Mycobacterium tuberculosis". Molecular ... "Acetyl-CoA-Mediated Activation of Mycobacterium tuberculosis Isocitrate Lyase 2". Nature Communications. 10 (1): 4639. Bibcode: ...
Daisy Roulland-Dussoix
"Conjugative transfer of a shuttle plasmid from Escherichia coli to Mycobacterium smegmatis [corrected]". FEMS Microbiol. Lett. ... Her publications from these years focused on mycobacterium and mycoplasmas, specifically genetic and molecular characterization ... "Restriction endonuclease mapping and cloning of Mycobacterium fortuitum var. fortuitum plasmid pAL5000". Ann Inst Pasteur ...
Trehalose O-mycolyltransferase
"Purification and characterization of a novel mycolic acid exchange enzyme from Mycobacterium smegmatis". J. Biol. Chem. 262 (28 ...
ClpX
... from Mycobacterium smegmatis". Scientific Reports. 9 (1): 18019. Bibcode:2019NatSR...918019N. doi:10.1038/s41598-019-53736-8. ... In Mycobacteria, the ClpXP protease is composed of the ATPase component (ClpX) and two ClpP proteins (ClpP1 and ClpP2). ... Schmitz KR, Carney DW, Sello JK, Sauer RT (October 2014). "Crystal structure of Mycobacterium tuberculosis ClpP1P2 suggests a ... Leodolter J, Warweg J, Weber-Ban E (2015-05-01). Zeth K (ed.). "The Mycobacterium tuberculosis ClpP1P2 Protease Interacts ...
Propionyl-CoA
"Role of the methylcitrate cycle in propionate metabolism and detoxification in Mycobacterium smegmatis". Microbiology. 153 (Pt ... In Mycobacterium tuberculosis, it has been suggested that the metabolism of propionyl-CoA is involved in cell wall biogenesis. ... It is also attributed as a resulting pathway due to the catabolism of fatty acids in mycobacteria. In order to proceed, the ... Muñoz-Elías EJ, Upton AM, Cherian J, McKinney JD (June 2006). "Role of the methylcitrate cycle in Mycobacterium tuberculosis ...
Umesh Varshney
... from Mycobacterium smegmatis". Journal of Structural Biology. 196 (3): 448-454. doi:10.1016/j.jsb.2016.09.012. PMID 27659385. ... His researches are known to have assisted in a wider understanding of the biology of mycobacterium, more specifically the ... Science and Technology and is known for his studies on protein synthesis and DNA repair in Escherichia coli and Mycobacterium ... researches are primarily focused in the area of protein synthesis and DNA repair in Escherichia coli and Mycobacterium ...
Low complexity regions in proteins
Kushwaha, Ambuj K.; Grove, Anne (2013-02-01). "C-terminal low-complexity sequence repeats of Mycobacterium smegmatis Ku ...
Globin
... of Mycobacterium smegmatis". FEBS Lett. 580 (17): 4031-41. doi:10.1016/j.febslet.2006.06.037. PMID 16814781. Yeh DC, ... Pathania R, Navani NK, Rajamohan G, Dikshit KL (May 2002). "Mycobacterium tuberculosis hemoglobin HbO associates with membranes ...
N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase
... a key gene in the mycothiol biosynthesis pathway in Mycobacterium smegmatis". Microbiology. 149 (Pt 5): 1341-9. doi:10.1099/mic ... from Mycobacterium tuberculosis reveals a zinc hydrolase with a lactate dehydrogenase fold". The Journal of Biological ...
Undecaprenyl-phosphate mannosyltransferase
Sources of this enzyme includes Micrococcus luteus, Phaseolus aureus, Mycobacterium smegmatis and cotton fibers. Lahav M, Chiu ...
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase
Tremblay LW, Fan F, Vetting MW, Blanchard JS (December 2008). "The 1.6 A crystal structure of Mycobacterium smegmatis MshC: the ... "Steady-state and pre-steady-state kinetic analysis of Mycobacterium smegmatis cysteine ligase (MshC)". Biochemistry. 46 (40): ... glutathione-S-transferase and maltose binding protein fusion proteins in Mycobacterium smegmatis" (PDF). Protein Expression and ...
DNA sequencing
Protein nanopore sequencing utilizes membrane protein complexes such as α-hemolysin, MspA (Mycobacterium smegmatis Porin A) or ...
Mycobacterium bovis
M. smegmatis group. *M. agri/M. thermoresistibile. *M. duvalii/M. flavescens. *M. monacense ... Mycobacterium bovis. Karlson & Lessel 1970,[1] ATCC 19210. Mycobacterium bovis (M. bovis) is a slow-growing (16- to 20-hour ... Mycobacterium bovis can be transmitted from human to human; there was an outbreak in Birmingham, England in 2004,[33] and from ... Karlson, A. G.; Lessel, E. F. (1970). "Mycobacterium bovis nom. nov". International Journal of Systematic Bacteriology. 20 (3 ...
Desiccation
Mycobacterium smegmatis mutant strains that are deficient in the ability to repair double-strand breaks by the non-homologous ... "NHEJ protects mycobacteria in stationary phase against the harmful effects of desiccation" (PDF). DNA Repair (Amst.). 6 (9): ...
Liksoza - Википедија, слободна енциклопедија
Chemistry of the Lyxose-Containing Mycobacteriophage Receptors of Mycobacterium phlei/Mycobacterium smegmatis, Biochemistry, 35 ...
Microbial genetics
... but also occurs in other bacteria such as Mycobacterium smegmatis. Conjugation requires stable and extended contact between a ... "Distributive conjugal transfer in mycobacteria generates progeny with meiotic-like genome-wide mosaicism, allowing mapping of ...
Bacterial morphological plasticity
... not only in this species but also in Mycobacterium smegmatis). The bacterial filament ultrastructure is consistent with ... A similar mechanism may occur in Mycobacterium tuberculosis,which also elongates after being phagocytized. Ssd encoded by ... England, Kathleen; Rebecca Crew; Richard A Slayden (2011). "Mycobacterium tuberculosis septum site determining protein, Ssd ...
Syzygium samarangense
They show weak antibiotic action against Staphylococcus aureus, Mycobacterium smegmatis, and Candida albicans.[5] ...
分枝杆菌属 - 维基百科,自由的百科全
M. smegmatis(英语:Mycobacterium smegmatis) *M. goodii(英语:Mycobacterium goodii) ... M. canetti(英语:Mycobacterium canetti). M. caprae(英语:Mycobacterium caprae). M. microti(英语:Mycobacterium microti). M. pinnipedii(英 ... M. indicus pranii(英语:Mycobacterium indicus pranii). 戈登分枝杆菌 Mycobacterium gordonae clade编辑. *M. asiaticum(英语:Mycobacterium ... M. kansasii(英语:Mycobacterium kansasii). Mycobacterium nonchromogenicum/terrae clade编辑. *M. hiberniae(英语:Mycobacterium
Mycobacterium avium complex
M. smegmatis group. *M. agri/M. thermoresistibile. *M. duvalii/M. flavescens. *M. monacense ... Mycobacterium avium complex is a group of mycobacteria comprising Mycobacterium intracellulare, Mycobacterium avium, and ... Pathogenic mycobacteria. Advances in Tuberculosis Research, 14, 235-287.. *^ "Mycobacterium Avium Complex. MAI; MAC Information ... These bacteria cause disease in humans called Mycobacterium avium-intracellulare infection or Mycobacterium avium complex ...
Terapevtska glina - Wikipedija, prosta enciklopedija
... smegmatis, in to kar za tisočkrat v primerjavi s celičnimi kulturami brez dodanih mineralnih produktov.«[12] ... povzroča pa je bakterija Mycobacterium ulcerans. ...
Mycobacterium lepromatosis
M. smegmatis group. *M. agri/M. thermoresistibile. *M. duvalii/M. flavescens. *M. monacense ... Mycobacterium lepromatosis is a bacterium that, along with Mycobacterium leprae, causes leprosy (Hansen's disease). It was only ... "Comparative Sequence Analysis of Mycobacterium leprae and the New Leprosy-Causing Mycobacterium lepromatosis". Journal of ... "A New Mycobacterium Species Causing Diffuse Lepromatous Leprosy". American Journal of Clinical Pathology. 130 (6): 856-64. doi ...
Koenzim F420 - Википедија, слободна енциклопедија
Posebno bogat prirodni izvor F420 je Mycobacterium smegmatis, u kojoj nekoliko desetina enzima koristi F420 umesto srodnog ... Selengut JD, Haft DH (2010). „Unexpected abundance of coenzyme F(420)-dependent enzymes in Mycobacterium tuberculosis and other ...
Acid-fastness
All Mycobacteria - M. tuberculosis, M. leprae, M. smegmatis and atypical Mycobacterium. *Actinomycetes (especially some aerobic ... The acid-fastness of Mycobacteria is due to the high mycolic acid content of their cell walls, which is responsible for the ... Some, such as Mycobacteria, can be stained with the Gram stain, but they do not take the crystal violet well and thus appear ... Acid-fast Mycobacteria can also be visualized by fluorescence microscopy using specific fluorescent dyes (auramine-rhodamine ...
Mycobacterium goodii
... was previously known as Mycobacterium smegmatis group 2. Brown et al. 1999. Mycobacterium wolinskyi sp. ... and Mycobacterium goodii sp. nov., two new rapidly growing species related to Mycobacterium smegmatis and associated with human ... Mycobacterium goodii is an acid-fast bacterial species in the phylum Actinobacteria and the genus Mycobacterium. M. goodii ... M. goodii is found in many of the same settings as M. smegmatis and members of the M. fortuitum complex. It can cause post- ...
Handkea utriformis
... and Mycobacterium smegmatis. On the other hand, H. utriformis has low antifungal activity against the species Candida albicans ...
Starch synthase (maltosyl-transferring)
2 The enzyme from the bacterium Mycobacterium smegmatis is specific for maltose. Elbein AD, Pastuszak I, Tackett AJ, Wilson T, ...
Cyclic di-AMP
... is a cyclic di-AMP-responsive repressor in Mycobacterium smegmatis". The Journal of Biological Chemistry. 288 (5): 3085-96. doi ... Cyclic di-AMP has been linked to fatty acid synthesis regulation in Myobacterium smegmatis, the growth of S. aureus in ...
Desiccation
Mycobacterium smegmatis mutant strains that are deficient in the ability to repair double-strand breaks by the non-homologous ... Pitcher RS, Green AJ, Brzostek A, Korycka-Machala M, Dziadek J, Doherty AJ (2007). "NHEJ protects mycobacteria in stationary ...
Mycobacterium
... smegmatis M. goodii M. wolinskyi M. sphagni M. thermoresistibile M. vanbaalenii M. arosiense M. aubagnense M. chlorophenolicum ... Structures of Mycobacterium tuberculosis proteins MycDB: Mycobacterium database TBDB: Tuberculosis database Mycobacterium ... Mycobacteria appear phenotypically most closely related to members of Nocardia, Rhodococcus, and Corynebacterium. Mycobacteria ... Runyon's group I, II and III Also see main article about Mycobacterium tuberculosis complex Mycobacterium tuberculosis complex ...
Ms1 small RNA
Mycobacterium smegmatis small RNA 1 (Ms1 small RNA) is highly expressed during stationary phase of growth, Ms1 RNA directly ... There is evidence that Ms1 RNA may function similar to 6S RNA in M. smegmatis which does not have 6S RNA. Hnilicová J, Jirát ... a novel sRNA interacting with the RNA polymerase core in mycobacteria". Nucleic Acids Research. 42 (18): 11763-11776. doi: ...
Mycofactocin
Bruender NA, Bandarian V (2017). "The Creatininase Homolog MftE from Mycobacterium smegmatis Catalyzes a Peptide Cleavage ... The name "mycofactocin" is derived from three words, the genus name "Mycobacterium" (across which it is nearly universal), " ... However, its species distribution is heavily skewed towards the Actinobacteria, including Mycobacterium tuberculosis, which is ... family alone in Mycobacterium avium. The mycofactocin biosynthesis pathway is one of the most abundant of any RiPP system in ...
Mycobacterium smegmatis - Redorbit
Mycobacterium smegmatis is 3.0 to 5.0 µm long with a bacillus shape, an acid-fast bacterial species in the phylum ... M. smegmatis is useful for the research analysis of other Mycobacteria species in laboratory experiments. Since it is a fast ... The M. smegmatis strain is now the work-horse of mycobacterial genetics. It is readily available in most laboratory media and ... This organism was later named M. smegmatis. It is considered a non-pathogenic microorganism although, in rare cases, it can ...
Mycobacterium smegmatis - Wikipedia
Mycobacterium smegmatis is an acid-fast bacterial species in the phylum Actinobacteria and the genus Mycobacterium. It is 3.0 ... M. smegmatis is useful for the research analysis of other Mycobacteria species in laboratory experiments. M. smegmatis is ... Information and photo from NCBI MicrobeWiki page on M. smegmatis Type strain of Mycobacterium smegmatis at BacDive - the ... I. Species descriptions of Mycobacterium phlei Lehmann and Neumann and Mycobacterium smegmatis (Trevisan) Lehmann and Neumann ...
RCSB PDB - 4XVO: L,D-transpeptidase from Mycobacterium smegmatis
Mycobacterium smegmatis (Trevisan) Lehmann and Neumann ATCC ® 607&
Mycobacterium smegmatis ATCC ® 607™ Designation: TypeStrain=False Application: Assay of bleomycin Bacteriophage host ... Nucleotide (GenBank) : X60513 M.smegmatis gltA gene for citrate synthase. Nucleotide (GenBank) : U07954 Mycobacterium smegmatis ... I. Species descriptions of Mycobacterium phlei Lehmann and Neumann and Mycobacterium smegmatis (Trevisan) Lehmann and Neumann ... Mycobacterium smegmatis (Trevisan) Lehmann and Neumann (ATCC® 607™) Type Strain: no / Biosafety Level: 1 ...
Cytokine-modified Mycobacterium smegmatis as a novel anticancer immunotherapy. - PubMed - NCBI
Mycobacterium smegmatis, and a TNFalpha gene-modified recombinant M. smegmatis. When wild-type M. smegmatis were delivered to ... Cytokine-modified Mycobacterium smegmatis as a novel anticancer immunotherapy.. Young SL1, Murphy M, Zhu XW, Harnden P, ... Recombinant M. smegmatis secreting TNFalpha, however, gave a 70% durable tumour-free survival. Lymphocytes from draining lymph- ... No toxicity was observed with wild-type or recombinant M. smegmatis in immunocompetent, T-deficient or NK-deficient models. We ...
Mycobacterium smegmatis (Trevisan 1889) Lehmann & Neumann 1899 articles
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Encyclopedia of Life
Mycobacterium smegmatis ( German ) provided by wikipedia DE Wissenschaftlicher Name Mycobacterium smegmatis (Trevisan 1889) ... Mycobacterium smegmatis: Brief Summary ( German ) provided by wikipedia DE Mycobacterium smegmatis ist ein Bakterium aus der ... Mycobacterium smegmatis ist eine Art in der Gattung Mycobacterium in der Familie der Mycobacteriaceae, die zur Abteilung ... Zusammen mit Mycobacterium goodii wird es in die Mycobacterium smegmatis-Clade gestellt.[1] ...
pckG - Phosphoenolpyruvate carboxykinase [GTP] - Mycobacterium smegmatis - pckG gene & protein
Mycobacterium vaccae ATCC 25954. Mycobacterium sp. SWH-M1. Mycobacterium peregrinum. Mycobacterium mucogenicum. Mycobacterium ... Mycobacterium boenickei. Mycobacterium septicum. Mycobacterium sp. NAZ190054. Mycobacterium bacteremicum. Mycobacterium ... Mycobacterium sp. UNC267MFSha1.1M11. Mycobacterium bacteremicum. Mycobacterium sp. Soil538. Mycobacterium sp. NAZ190054. And ... Mycobacterium gilvum (strain DSM 45189 / LMG 24558 / Spyr1). Mycobacterium vanbaalenii (strain DSM 7251 / PYR-1). Mycobacterium ...
New tRNA contacts facilitate ligand binding in a Mycobacterium smegmatis T box riboswitch | PNAS
New tRNA contacts facilitate ligand binding in a Mycobacterium smegmatis T box riboswitch. Anna V. Sherwood, Jane K. Frandsen, ... In this study, the role of the conserved elements of the Mycobacterium smegmatis ileS T box riboswitch in tRNAIle binding was ... In this study, structural elements of a Mycobacterium smegmatis ileS T box riboswitch variant with an Ultrashort (US) Stem I ... New tRNA contacts facilitate ligand binding in a Mycobacterium smegmatis T box riboswitch ...
RCSB PDB - 3NDO: Crystal structure of deoxyribose phosphate aldolase from mycobacterium smegmatis
Crystal structure of deoxyribose phosphate aldolase from mycobacterium smegmatis. *DOI: 10.2210/pdb3NDO/pdb ... Mycolicibacterium smegmatis. Mutation(s): 0 Gene Names: deoC, MSMEG_0922. EC: 4.1.2.4. ... Find proteins for A0QQY7 (Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)) ... High-resolution three-dimensional structures of essential Mycobacterium tuberculosis (Mtb) proteins provide templates for TB ...
gyrB - DNA gyrase subunit B - Mycobacterium smegmatis - gyrB gene & protein
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155). Mycobacterium smegmatis. Mycobacterium smegmatis (strain MKD8). 675. ... Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155). Mycobacterium smegmatis. Mycobacterium smegmatis (strain MKD8). ... Mycobacterium sp. 1274761.0. Mycobacterium sp. 1482268.1. Mycobacterium sp.. Mycobacterium flavescens. Mycobacterium sp. GA- ... Mycobacterium smegmatis. ,p>This subsection of the Names and taxonomy section shows the unique identifier assigned by the , ...
The dUTPase Enzyme Is Essential in Mycobacterium smegmatis
Using Mycobacterium smegmatis as a fast growing model for Mycobacterium tuberculosis, we demonstrate that dUTPase knock-out ... In addition, here we demonstrated that Mycobacterium tuberculosis dUTPase is fully functional in Mycobacterium smegmatis as it ... We also show that deletion of the mycobacteria-specific loop has no major effect on dUTPase enzymatic properties in vitro and ... All mycobacterial genomes encode dUTPase with a mycobacteria-specific surface loop absent in the human dUTPase. ...
Isolation problems and structural organization of membrane units in Mycobacterium sp. smegmatis | SpringerLink
smegmatis were isolated in a biological active state by diff ... spheroplasts induced by lysozyme or glycine from Mycobacterium ... Isolation problems and structural organization of membrane units in Mycobacterium sp. smegmatis *P. Mišoň1. , ... Membrane units from lysed spheroplasts induced by lysozyme or glycine from Mycobacterium spec. smegmatis were isolated in a ... Mišoň, P., Trnka, L., Mohelská, H. et al. Isolation problems and structural organization of membrane units in Mycobacterium sp ...
KEGG PATHWAY: Nucleotide excision repair - Mycobacterium smegmatis MC2 155
Efflux pump of the proton antiporter family confers low-level fluoroquinolone resistance in Mycobacterium smegmatis | PNAS
By using the rapid-growing Mycobacterium smegmatis as a model genetic system, a gene was selected that confers low-level FQ ... Efflux pump of the proton antiporter family confers low-level fluoroquinolone resistance in Mycobacterium smegmatis. H E Takiff ... Efflux pump of the proton antiporter family confers low-level fluoroquinolone resistance in Mycobacterium smegmatis ... Efflux pump of the proton antiporter family confers low-level fluoroquinolone resistance in Mycobacterium smegmatis ...
IS1549 from Mycobacterium smegmatis Forms Long Direct Repeats upon Insertion | Journal of Bacteriology
IS1549 from Mycobacterium smegmatis Forms Long Direct Repeats upon Insertion. Bonnie B. Plikaytis, Jack T. Crawford, Thomas M. ... IS1549 from Mycobacterium smegmatis Forms Long Direct Repeats upon Insertion. Bonnie B. Plikaytis, Jack T. Crawford, Thomas M. ... IS1549 from Mycobacterium smegmatis Forms Long Direct Repeats upon Insertion. Bonnie B. Plikaytis, Jack T. Crawford, Thomas M. ... IS1549 from Mycobacterium smegmatis Forms Long Direct Repeats upon Insertion Message Subject (Your Name) has forwarded a page ...
The specialized secretory apparatus ESX-1 is essential for DNA transfer in Mycobacterium smegmatis. - PubMed - NCBI
The specialized secretory apparatus ESX-1 is essential for DNA transfer in Mycobacterium smegmatis.. Coros A1, Callahan B, ... Conjugal DNA transfer in Mycobacterium smegmatis occurs by a mechanism distinct from plasmid-mediated DNA transfer. Previously ... The Specialized secretory apparatus ESX-1 is essential for DNA transfer in Mycobacterium smegmatis ... The Specialized secretory apparatus ESX-1 is essential for DNA transfer in Mycobacterium smegmatis ...
Paralogous cAMP receptor proteins in Mycobacterium smegmatis show biochemical and functional divergence. | Sigma-Aldrich
Mycobacterium smegmatis LfrA protein
Summary Report | CureHunter
... proton antiporter isolated from Mycobacterium smegmatis; amino acid sequence in first source; GenBank U40487 ... Mycobacterium smegmatis LfrA protein. Subscribe to New Research on Mycobacterium smegmatis LfrA protein ... proton antiporter isolated from Mycobacterium smegmatis; amino acid sequence in first source; GenBank U40487 ...
Frontiers | Mycobacterium smegmatis PhoU Proteins Have Overlapping Functions in Phosphate Signaling and Are Essential |...
Mycobacterium smegmatis serves as a model for studying mycobacterial pathogens including M. tuberculosis. M. smegmatis encodes ... M. smegmatis serves as a model for studying mycobacterial pathogens including M. tuberculosis. M. smegmatis encodes two ... Mycobacterium tuberculosis, the causative agent of tuberculosis, requires its phosphate sensing signal transduction system for ... Our results suggest that M. smegmatis can serve as a tractable model for further characterization of the molecular mechanism of ...
NADH Dehydrogenase Defects Confer Isoniazid Resistance and Conditional Lethality in Mycobacterium smegmatis | Journal of...
... smegmatis and Mycobacterium bovisBCG and the integrative cosmid pYUB412 (AprHygr) (4). The M. smegmatis library was a gift from ... NADH Dehydrogenase Defects Confer Isoniazid Resistance and Conditional Lethality in Mycobacterium smegmatis Lynn Miesel, Torin ... 1990) Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis. Mol. Microbiol. 4: ... 1994) Effects of peroxides on susceptibilities of Escherichia coli and Mycobacterium smegmatis to isoniazid. Antimicrob. Agents ...
Search results for: 'Mycobacterium smegmatis'
MSMEG_2964 Recombinant Protein (Mycobacterium smegmatis) (OPCA13159) Catalog #: OPCA13159 Predicted Species: Mycobacterium ... MSMEG_4483 Recombinant Protein (Mycobacterium smegmatis) (OPCA186814) Catalog #: OPCA186814 Predicted Species: Mycobacterium ... MSMEG_6896 Recombinant Protein (Mycobacterium smegmatis) (OPCA321113) Catalog #: OPCA321113 Predicted Species: Mycobacterium ... GLTA Recombinant Protein (Mycobacterium smegmatis) (OPCA16873) Catalog #: OPCA16873 Predicted Species: Mycobacterium smegmatis ...
The growth rate of Mycobacterium smegmatis depends on sufficient porin-mediated influx of nutrients. | Sigma-Aldrich
MspA is the major porin of Mycobacterium smegmatis. In this study we showed that three paralogues of MspA, namely MspB, MspC ... The growth rate of Mycobacterium smegmatis depends on sufficient porin-mediated influx of nutrients.. [Joachim Stephan, ... Mycobacteria have a unique outer membrane (OM) that is thicker than any other known biological membrane. Nutrients cross this ... It is concluded that mspB and mspD provide backup porins for M. smegmatis. This also indicated that a minimal porin-mediated OM ...
Frontiers | Dissecting the Mechanism of Intracellular Mycobacterium smegmatis Growth Inhibition by Platelet Activating Factor C...
Mycobacterium bovis BCG and Mycobacterium smegmatis (M. smegmatis), by damaging the bacterial cell membrane. Here, we have ... Mycobacterium bovis BCG and Mycobacterium smegmatis (M.smegmatis), by damaging the bacterial cell membrane. Here, we have ... Our results demonstrated that exogenous PAF C-16 inhibited the growth of M.smegmatis inside phagocytic cells of monocytic cell ... Our results demonstrated that exogenous PAF C-16 inhibited the growth of M. smegmatis inside phagocytic cells of monocytic cell ...
Essential Gene(s) Targeted by Peptide Nucleic Acids Kills Mycobacterium smegmatis in Culture and in Infected Macrophages
The PNAs targeting the pncA, polA and rpoC genes were found to exhibit strong growth inhibition against M. smegmatis but only ... No growth inhibition of M. smegmatis was seen in pure culture when treated with PNAs targeting gyrA and a mismatch PNA ... Results: In Middlebrook broth, the strong growth inhibitory effect against M. smegmatis was observed by PNAs targeting the inhA ... the efficacy of several antisense PNAs in inhibiting extracellular and intracellular growth of Mycobacterium smegmatis. Methods ...
Crystal Structure of PhnF, a GntR-family Transcriptional Regulator of Phosphate Transport in Mycobacterium Smegmatis
Mycobacterium smegmatis possesses two such transporters, the widely distributed PstSCAB system an … ... Mycobacterium smegmatis possesses two such transporters, the widely distributed PstSCAB system and PhnDCE, a transporter that ... Crystal Structure of PhnF, a GntR-family Transcriptional Regulator of Phosphate Transport in Mycobacterium Smegmatis J ... In contrast, pathogenic mycobacteria were not found to encode Phn-like systems but instead were found to possess multiple ...
MSMEG 6441 (Mycobacterium smegmatis str. MC2 155) | Gene Target - PubChem
Biochemical characterization of the 49 kDa penicillin-binding protein of Mycobacterium smegmatis | Biochemical Journal |...
Biochemical characterization of the 49 kDa penicillin-binding protein of Mycobacterium smegmatis Tapan MUKHERJEE Tapan ... The 49 kDa penicillin-binding protein (PBP) of Mycobacterium smegmatis catalyses the hydrolysis of the peptide or S-ester bond ... Biochemical characterization of the 49 kDa penicillin-binding protein of Mycobacterium smegmatis. Biochem J 15 November 1996; ...
Intracellular accumulation of norfloxacin in Mycobacterium smegmatis. | Antimicrobial Agents and Chemotherapy
To evaluate the intracellular accumulation of norfloxacin in mycobacteria, two methods were used with Mycobacterium smegmatis. ... Intracellular accumulation of norfloxacin in Mycobacterium smegmatis. Message Subject (Your Name) has forwarded a page to you ... Intracellular accumulation of norfloxacin in Mycobacterium smegmatis.. S Corti, J Chevalier, A Cremieux ... Thus, the same mechanisms of quinolone accumulation that occur in eubacteria seem to occur in mycobacteria, at least in M. ...
CiNii 論文 -
Structures of ADP-Ribosylated Rifampicin and Its Metabolite : Intermediates of...
Ribosylative inactivation of rifampin by Mycobacterium smegmatis is a principal contributor to its low susceptibility to this ... Intermediates of Rifampicin-ribosylation by Mycobacterium smegmatis DSM43756 * * MORISAKI Naoko ... and characterization of a new intermediate in the ribosylative inactivation pathway of rifampin by Mycobacterium smegmatis IMAI ...
Ergothioneine Is a Secreted Antioxidant in Mycobacterium smegmatis | Antimicrobial Agents and Chemotherapy
Ergothioneine Is a Secreted Antioxidant in Mycobacterium smegmatis. Carine Sao Emani, Monique J. Williams, Ian J. Wiid, ... Ergothioneine Is a Secreted Antioxidant in Mycobacterium smegmatis. Carine Sao Emani, Monique J. Williams, Ian J. Wiid, ... Ergothioneine Is a Secreted Antioxidant in Mycobacterium smegmatis. Carine Sao Emani, Monique J. Williams, Ian J. Wiid, ... Mycothiol import by Mycobacterium smegmatis and function as a resource for metabolic precursors and energy production. J. ...
TuberculosisMsmegOrganismSpeciesGenomeResponse of Mycobacterium smegmatis to nitrogenLepraeGenesBovisBacteriaProteinProteinsGeneMutantsRecombinant MycobacteDrug resistant strainsMutationPathogensCharacterizationInhibitionPathogenic mycobacteriaBiosynthesisSaprophyteMutantGenusMycobacterialDeletionGeneticPathwayMetabolismNonpathogenicEnzymeMutationsHigh-affinityPathwaysGrowthMacrophagesResistanceStrainBiochemicalPhosphate TransportEssentialCharacterizeInhibitory effectsMechanismBacterialWidelySurvivalActinobacteria
Tuberculosis71
- Mycobacterium smegmatis: an absurd model for tuberculosis? (eol.org)
- High-resolution three-dimensional structures of essential Mycobacterium tuberculosis (Mtb) proteins provide templates for TB drug design, but are available for only a small fraction of the Mtb proteome. (rcsb.org)
- Is the Subject Area "Mycobacterium tuberculosis" applicable to this article? (plos.org)
- Due to the resurgence of tuberculosis and the emergence of multidrug-resistant strains, fluoroquinolones (FQ) are being used in selected tuberculosis patients, but FQ-resistant strains of Mycobacterium tuberculosis have rapidly begun to appear. (pnas.org)
- DNA transfer therefore provides a simple molecular genetic assay to characterize ESX-1, which, in Mycobacterium tuberculosis, is necessary for full virulence. (nih.gov)
- Moreover, our observation that ESX-1 has such diametrically opposed effects on transfer in the donor and recipient, forces us to consider how proteins secreted by the ESX-1 apparatus can function so as to modulate two seemingly disparate processes, M. smegmatis DNA transfer and M. tuberculosis virulence. (nih.gov)
- A genetic map comparing the esx-1 loci of M. smegmatis and M. tuberculosis . (nih.gov)
- Mycobacterium tuberculosis , the causative agent of tuberculosis, requires its phosphate sensing signal transduction system for virulence and antibiotic tolerance, but the molecular mechanisms of phosphate sensing remain poorly characterized. (frontiersin.org)
- Isoniazid (INH) is a highly effective drug used in the treatment and prophylaxis of Mycobacterium tuberculosis infections. (asm.org)
- Mycobacterium tuberculosis is exquisitely sensitive to the drug isoniazid (INH), and this sensitivity has made INH the core of tuberculosis chemotherapy and prophylaxis since the early 1950s ( 1 , 37 ). (asm.org)
- In our effort to develop better tuberculosis drugs, we are trying to understand how INH kills sensitive mycobacteria and how mycobacteria become resistant. (asm.org)
- The InhA enzyme catalyzes an essential step in fatty acid elongation and mycolic acid synthesis and is a target for INH action in M. tuberculosis and the soil saprophyte Mycobacterium smegmatis ( 3 , 36 ). (asm.org)
- Mycobacterium tuberculosis ( M.tb ) infection results in approximately 1.3 million human deaths each year. (frontiersin.org)
- Mycobacterium tuberculosis ( M.tb ) belongs to the acid-fast group of bacteria and causes an infectious disease in humans, known as Tuberculosis (TB), which mostly affects the respiratory system. (frontiersin.org)
- Mycobacterium smegmatis is an avirulent bacterial genus present in the soil and smegma that shows many features similar to Mycobacterium tuberculosis ( Mtb ), responsible for causing tuberculosis in humans [1]. (scirp.org)
- The MSH-deficient Δ mshA mutant of Mycobacterium tuberculosis requires catalase during in vitro growth, implicating MSH in detoxifying reactive oxygen species ( 10 ). (asm.org)
- Because of this, Mycobacterium tuberculosis (Mtb) is more rapidly killed using a combination of drugs capable of PBP- and LDT- inhibition. (elifesciences.org)
- However, in a group of bacteria called mycobacteria, which cause tuberculosis and other diseases, the units are added at the tips. (elifesciences.org)
- But, drugs that treat tuberculosis do not attack this layer, partly because we know very little about the cell walls of mycobacteria. (elifesciences.org)
- In fact, there are important differences between model bacteria and Actinobacteria like mycobacteria, a genus of rod-shaped bacteria that includes the major human pathogen Mycobacterium tuberculosis (Mtb). (elifesciences.org)
- It contributes to the virulence of Mycobacterium tuberculosis. (jic.ac.uk)
- The recent resurgence of drug resistant strains of Mycobacterium tuberculosis, the causal agent of tuberculosis (TB) has strengthened the need for new anti-TB drugs. (bl.uk)
- Evaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studies. (sld.cu)
- Preliminary studies of humoral immunogenicity and cross-reactivity with M. tuberculosis in mice using two M. smegmatis- derived experimental vaccines were carried out, demonstrating the immunogenicity of M. smegmatis proteoliposomes and the recognition of M. tuberculosis proteins by the sera of animals immunized with this vaccine candidate. (sld.cu)
- The conjunction of in silico and in vivo studies suggested the potential for future evaluation of M. smegmatis as vaccine candidate against tuberculosis using different strategies. (sld.cu)
- En este estudio se revisaron múltiples bases de datos publicadas, relacionadas con experimentos de expresión de genes de M. tuberculosis in vivo en diferentes estadios de la infeccción en humanos y animales. (sld.cu)
- Se llevaron a cabo estudios de inmunogenicidad humoral y reactividad cruzada con M. tuberculosis en ratones inmunizados con dos vacunas experimentales obtenidas a partir de M. smegmatis , demostrándose la immunogenicidad de los proteoliposomas y el reconocimiento de proteínas de M. tuberculosis por el suero de ratones vacunados con este candidato vacunal. (sld.cu)
- Los resultados obtenidos con los estudios in sílico e in vivo sugieren la potencialidad para evaluación futura de candidatos vacunales obtenidos a partir de M. smegmatis para la prevención de la tuberculosis. (sld.cu)
- Mycobacterium tuberculosis , the main agent of tuberculosis (TB) in humans caused 9.2 million new cases and 1.7 million deaths in 2006. (sld.cu)
- With the complete genome sequence of M. tuberculosis and the advances in the sequencing of BCG and other mycobacteria, an enormous flow of valuable information has been made available for new vaccine strategies (2, 3). (sld.cu)
- We investigated the use of a tetracycline-inducible expression system in Mycobacterium tuberculosis and Mycobacterium smegmatis . (asm.org)
- The chromosomal regions surrounding the origins of DNA replication from M. smegmatis, M. tuberculosis, and M. leprae have been sequenced, and show very few differences. (epfl.ch)
- By means of subcloning experiments, the putative chromosomal origin of replication of M. smegmatis, containing the dnaA-dnaN region, was shown to promote autonomous replication in M. smegmatis, unlike the corresponding regions from M. tuberculosis or M. leprae. (epfl.ch)
- The truncated Rv2820c of Mycobacterium tuberculosis Beijing family augments intracellular. (deepdyve.com)
- Bao, Lang 2018-04-01 00:00:00 Genetic variations among genes of Mycobacterium tuberculosis may be associated with antigenic variation and immune evasion, which complicates the pathogenesis of M. tuberculosis. (deepdyve.com)
- It has been reported that Rv2820c-Bj correlated with enhanced intracellular survival of M. smegmatis in macrophages when compared to its full-length counterpart Rv2820c in M. tuberculosis, however, the respective contribution of the truncation and the new 3′-end of Rv2820c-Bj to this enhancement was unclear. (deepdyve.com)
- Recombinant Mycobacterium smegmatis expressing an ESAT6-CFP10 fusion protein induces anti-mycobacterial immune responses and protects against Mycobacterium tuberculosis challenge in mice. (semanticscholar.org)
- Recombinant Bacillus subtilis spores for the delivery of Mycobacterium tuberculosis Ag85B-CFP10 secretory antigens. (semanticscholar.org)
- The mc2-CMX vaccine induces an enhanced immune response against Mycobacterium tuberculosis compared to Bacillus Calmette-Guérin but with similar lung inflammatory effects. (semanticscholar.org)
- Prime-boost vaccination with Bacillus Calmette Guerin and a recombinant adenovirus co-expressing CFP10, ESAT6, Ag85A and Ag85B of Mycobacterium tuberculosis induces robust antigen-specific immune responses in mice. (semanticscholar.org)
- Mycobacterium tuberculosis (Mtb) and Mycobacterium smegmatis (Msmeg) can grow on cholesterol as the sole carbon source. (escholarship.org)
- The serine/threonine kinase PknB of Mycobacterium tuberculosis phosphorylates PBPA, a penicillin-binding protein required for cell division. (microbiologyresearch.org)
- Contribution of the Mycobacterium tuberculosis MmpL protein family to virulence and drug resistance. (microbiologyresearch.org)
- Inhibition of mycolic acid transport across the Mycobacterium tuberculosis plasma membrane. (microbiologyresearch.org)
- Therapeutic potential of the Mycobacterium tuberculosis mycolic acid transporter, MmpL3. (microbiologyresearch.org)
- Mycobacterium smegmatis is a saprophytic bacterium frequently used as a genetic surrogate to study pathogenic Mycobacterium tuberculosis . (biomedcentral.com)
- In this study, transcriptomic analysis (RNA-seq) of an M. smegmatis Δ prrAB mutant was used to define the PrrAB regulon and provide insights into the essential nature of PrrAB in M. tuberculosis . (biomedcentral.com)
- PrrAB induced transcription of dosR ( devR ), a response regulator gene that promotes latent infection in M. tuberculosis and 21 of the 25 M. smegmatis DosRS regulon homologues. (biomedcentral.com)
- Mycobacterium tuberculosis , the causative agent of tuberculosis, is an ancient disease of mankind and the leading cause of death from an infectious agent [ 12 ]. (biomedcentral.com)
- The role of several energy-driven transport systems and the effects of anti- tubercular compounds on cellular and ion homeostasis in M. smegmatis and Mycobacterium tuberculosis were also studied. (otago.ac.nz)
- MSH is produced by Mycobacterium tuberculosis, members of the Actinomycetes family, to maintain an intracellular reducing environment and protect against oxidative and antibiotic induced stress. (elsevier.com)
- However, porins that could mediate the diffusion of phosphates across the OM of M. bovis BCG and Mycobacterium tuberculosis are unknown. (uab.edu)
- 3. Rao V , Gao F , Chen B , Jacobs WR , Glickman MS . Trans-cyclopropanation of mycolic acids on trehalose dimycolate suppresses Mycobacterium tuberculosis -induced inflammation and virulence. (microbiologyresearch.org)
- The anti-tuberculosis drug, ethambutol (Emb), was previously shown to inhibit the synthesis of arabinans of both the cell wall arabinogalactan (AG) and lipoarabinomannan (LAM) of Mycobacterium tuberculosis and other mycobacteria. (semanticscholar.org)
- In this report the characterization of complexes of Ugi with UDGs from Mycobacterium tuberculosis, a pathogenic bacterium, and Mycobacterium smegmatis, a widely used model organism for the former, is described. (iisc.ernet.in)
- However, the M. tuberculosis (Mtu) UDG-Ugi complex was seen when MtuUDG was incubated with M. smegmatis (Msm) UDG-Ugi or EcoUDG(L191G)-Ugi complexes. (iisc.ernet.in)
- The ESAT-6 (early secreted antigenic target, 6 kDa) family collects small mycobacterial proteins secreted by Mycobacterium tuberculosis , particularly in the early phase of growth. (beds.ac.uk)
- In this research, we performed EMSA experiments and transcriptional analysis of ESAT-6 cluster 3 in Mycobacterium smegmatis ( msmeg0615 - msmeg0625 ) and M. tuberculosis . (beds.ac.uk)
- In contrast to what we had observed in M. tuberculosis , we found that in M. smegmatis ESAT-6 cluster 3 responds only to iron and not to zinc. (beds.ac.uk)
- In contrast with what has been reported for M. tuberculosis , our results suggest that in M. smegmatis only IdeR-dependent regulation is retained, while zinc has no effect on gene expression. (beds.ac.uk)
- Differences in expression between cluster 3 genes can be explained by the presence of internal promoters, both in M. smegmatis and M. tuberculosis . (beds.ac.uk)
- The gene encoding methionine adenosyltransferase has been cloned from Mycobacterium tuberculosis and the model organism M. smegmatis . (beds.ac.uk)
- While the M. smegmatis enzyme could be functionally expressed, the M. tuberculosis homologue was insoluble and inactive under a large variety of expression conditions. (beds.ac.uk)
- Mycobacterium tuberculosis is difficult to kill for a number of reasons. (beds.ac.uk)
- In the examination of biochemical pathways in Mycobacterium tuberculosis , it would be ideal to identify processes where an enzyme plays a role in both active and chronic phase survival. (beds.ac.uk)
- Interaction of Erp Protein of Mycobacterium tuberculosis with Rv2212 Enhances Intracellular Survival of Mycobacterium smegmatis. (osdd.net)
- The Mycobacterium tuberculosis exported repetitive protein (RvErp) is a crucial virulence-associated factor as determined by its role in the survival and multiplication of mycobacteria in cultured macrophages and in vivo Although attempts have been made to understand the function of Erp protein, its exact role in Mycobacterium pathogenesis is still elusive. (osdd.net)
- Tuberculosis, caused by Mycobacterium tuberculosis, is a highly infectious disease. (wpi.edu)
- Therefore, immediate discovery of new antibiotic compounds is crucial for counteracting the evolving antibiotic resistance in strains of Mycobacterium tuberculosis and related species. (smu.ca)
- In previous studies, liposomes obtained from lipids of Mycobacterium smegmatis (LMs) demonstrated their immunogenicity and protective capacity against Mycobacterium tuberculosis in mice. (who.int)
- Sutherlandia is prescribed for people with tuberculosis but it is still not known what compounds in this plant act against Mycobacterium tuberculosis and its mode of action. (satnt.ac.za)
Msmeg5
- these genes are related to M. smegmatis genes Msmeg_0055-0058 as indicated by their colours. (nih.gov)
- MSMEG_6441 (Mycobacterium smegmatis str. (nih.gov)
- A mutant of M. smegmatis lacking egtD ( MSMEG_6247 ) was unable to synthesize ERG, confirming its role in ERG biosynthesis. (asm.org)
- Mycobacterium smegmatis (MSMEG) is an environmental, nonpathogenic Mycobacterium that is widely used as a model organism to study mycobacterial metabolism and pathogenicity. (asm.org)
- The Erp protein of Mycobacterium smegmatis (MSMEG_6405, or MsErp) interacts neither with Rv2212 nor with Ms_4279, the M. smegmatis homologue of Rv2212. (osdd.net)
Organism4
- This organism was later named M. smegmatis. (redorbit.com)
- As any immunotherapy involving administration of live BCG organisms (wild-type or recombinant) is likely to have associated toxicity (notably in the immunocompromised), we examined the anti-tumour potential of the closely related nonpathogenic organism, Mycobacterium smegmatis, and a TNFalpha gene-modified recombinant M. smegmatis. (nih.gov)
- Two possible candidates that could be responsible for maintaining high DNA fidelity in this MMR-deficient organism are the ancestral-like DNA polymerase DnaE1, which contains a highly efficient DNA proofreading histidinol phosphatase (PHP) domain, and/or the existence of a uracil-DNA glycosylase B (UdgB) homolog that might protect the GC-rich M. smegmatis genome against DNA damage arising from oxidation or deamination. (g3journal.org)
- However, since Mycobacterium is a slower growing organism, the differences between the 5-day and the 7-day biofilms are difficult to ascertain. (asmscience.org)
Species18
- Mycobacterium smegmatis is 3.0 to 5.0 µm long with a bacillus shape, an acid-fast bacterial species in the phylum Actinobacteria. (redorbit.com)
- M. smegmatis is useful for the research analysis of other Mycobacteria species in laboratory experiments. (redorbit.com)
- Mycobacterium smegmatis is an acid-fast bacterial species in the phylum Actinobacteria and the genus Mycobacterium. (wikipedia.org)
- The time and heavy infrastructure needed to work with pathogenic species prompted researchers to use M. smegmatis as a model for mycobacterial species. (wikipedia.org)
- This suggests that transformation in M. smegmatis is a DNA repair process, presumably a recombinational repair process, as it is in other bacterial species. (wikipedia.org)
- I. Species' descriptions of Mycobacterium phlei Lehmann and Neumann and Mycobacterium smegmatis (Trevisan) Lehmann and Neumann. (atcc.org)
- None of 13 other species of Mycobacterium tested by PCR with two sets of primers specific for IS 1549 were positive for the expected amplified product. (asm.org)
- The genus Mycobacterium is composed of species with widely differing growth rates ranging from approximately three hours in Mycobacterium smegmatis to two weeks in Mycobacterium leprae. (epfl.ch)
- The HIV pandemic raised the potential for facultative-pathogenic mycobacterial species like, Mycobacterium kansasii, to cause disseminating disease in humans with immune deficiencies. (www.gov.uk)
- In contrast, non-pathogenic mycobacterial species, like M. smegmatis, are not known to cause disseminating disease even in immunocompromised individuals. (www.gov.uk)
- A comparison of two rapid-growing, non-pathogenic species (M. smegmatis and M. fortuitum) with two facultative-pathogenic species (M. kansasii and M. bovis BCG) demonstrated that only the non-pathogenic bacteria induced strong apoptosis in human THP-1 cells and murine bone marrow-derived macrophages (BMDM) and dendritic cells (BMDD). (www.gov.uk)
- Several species of Mycobacteria can cause diseases in animals and humans. (asmscience.org)
- Since mycolic acid biosynthesis was affected, we cloned and expressed Mycobacterium smegmatis mycolic acid methyltransferases, postulated as targets for thiacetazone in other mycobacterial species. (microbiologyresearch.org)
- In mycobacteria, these regulators are encoded by an operon, whereas in other bacterial species, SmtB and Zur are encoded on separate loci. (asm.org)
- We show here, using Mycobacterium smegmatis as a model species, that a significant proportion of these ICDSs result from sequencing errors. (biomedcentral.com)
- We used M. smegmatis mc 2 155 as the model species for this study. (biomedcentral.com)
- The Actinomycetes, such as Mycobacterium and Corynebacterium species do not produce GSH and utilize instead mycothiol (MSH) as their alternative LMW thiol. (uni-greifswald.de)
- 7 days after the last exposure to able to infection by rapidly growing idly growing Mycobacterium species by sick or dead poultry ( 1-4 ). (cdc.gov)
Genome8
- The complete genome of M. smegmatis has been sequenced by TIGR, and microarrays have been produced by PFGRC program (http://pfgrc.tigr.org/descriptionPages.shtml), adding further to its use as a model system to study mycobacteria. (wikipedia.org)
- In addition to esx-1 genes, our screen identifies novel non-esx-1 loci in the M. smegmatis genome that are required for both DNA transfer and ESX-1 activity. (nih.gov)
- Here, we identify two paralogous CRPs in the genome of Mycobacterium smegmatis that have 78% identical sequences and characterize them biochemically and functionally. (sigmaaldrich.com)
- Conclusion: PitA is the only low-affinity phosphate transport system annotated in the genome of M. smegmatis. (uni-muenchen.de)
- A transposon mutant collection with an estimated 1.1-fold genome coverage (7680 mutants) was constructed in Mycobacterium smegmatis and screened in high-throughput against isoniazid. (nih.gov)
- Mycobacteria have a GC-rich genome and are slow growing and difficult to lyse because of their lipid-rich cell wall. (asm.org)
- Mutation accumulation experiments of M. smegmatis yielded a base-substitution mutation rate of 5.27 × 10 −10 per site per generation, or 0.0036 per genome per generation, which is surprisingly similar to the mutation rate in MMR-functional unicellular organisms. (g3journal.org)
- Previous work has reported the impact of VapC upon each gene in the M. smegmatis genome and produced a hypothesis model for the specific motif targeted by the enzyme. (waikato.ac.nz)
Response of Mycobacterium smegmatis to nitrogen1
- I found 26 transcriptional regulators that mediated the global transcriptomic response of Mycobacterium smegmatis to nitrogen limitation and I identified several non-coding RNAs that might be involved in the regulation of nitrogen- regulated gene expression. (otago.ac.nz)
Leprae1
- Convit, J.: Electron microscope study of Mycobacterium leprae and its environment in a vesicular leprous lesion. (springer.com)
Genes14
- U07955 Mycobacterium smegmatis ATCC 607 intergenic region between 16S and 23S rRNA genes. (atcc.org)
- Only the Δ phoU1 Δ phoU2 mutant exhibited constitutive activation of all the RegX3-regulated genes examined, suggesting that M. smegmatis PhoU1 and PhoU2 have overlapping functions in inhibiting activity of the SenX3-RegX3 two-component system when phosphate is readily available. (frontiersin.org)
- In Middlebrook broth, the strong growth inhibitory effect against M. smegmatis was observed by PNAs targeting the inhA and rpsL genes at all four concentrations. (scirp.org)
- The PNAs targeting the pncA , polA and rpoC genes were found to exhibit strong growth inhibition against M. smegmatis but only at 20 μM concentration. (scirp.org)
- To the best of our knowledge, no study has been performed so far to evaluate the growth inhibitory functions of anti-sense PNAs against Mycobacterium targeting one of its essential genes inside the macrophage. (scirp.org)
- Here we show that the phnDCE genes are common among environmental mycobacteria, where they are often associated with phnF-like genes. (nih.gov)
- In contrast, pathogenic mycobacteria were not found to encode Phn-like systems but instead were found to possess multiple copies of the pst genes. (nih.gov)
- Four genes are involved in MSH biosynthesis in mycobacteria, namely, mshA , mshB , mshC , and mshD , and mutants harboring deletions in mshB , mshC , and mshD produce different levels of MSH due to the ability of other enzymes to partially compensate for their loss ( 6 , 7 ). (asm.org)
- ERG biosynthetic genes ( egtA , egtB , egtC , egtD , and egtE ) were recently identified in M. smegmatis ( 11 ). (asm.org)
- We have demonstrated that GlnR is the global nitrogen response regulator in M. smegmatis, directly regulating the expression of more than 100 genes. (biomedcentral.com)
- A number of essential genes have been identified in mycobacteria, but methods to study these genes have not been developed, leaving us unable to determine the function or biology of the genes. (asm.org)
- Thus, methods for studying essential genes in mycobacteria are urgently required. (asm.org)
- In M. smegmatis , the expression of msmeg0615 and msmeg0620 genes is broadly similar in differing growth phases and in stress conditions, with the exception of acid stress (pH 4.2). (beds.ac.uk)
- Analysis of the genomic sequence of Mycobacterium smegmatis suggests the presence of several putative thiolase genes. (iisc.ac.in)
Bovis4
- We have recently shown that PAF C-16 can directly inhibit the growth of two representative non-pathogenic mycobacteria, Mycobacterium bovis BCG and Mycobacterium smegmatis ( M. smegmatis ), by damaging the bacterial cell membrane. (frontiersin.org)
- The current vaccine, Mycobacterium bovis Bacillus Calmette-Guerin (BCG) is the most widely used in humans. (sld.cu)
- The phosphatase activity in whole cells of Mycobacterium bovis BCG was significantly less than that in lysed cells, indicating a similar uptake pathway for phosphates in slow-growing mycobacteria. (uab.edu)
- 10. Sprott GD, Dicaire CJ, Gurnani K, Sad S, Krishnan L. Activation of dendritic cells by liposomes prepared from phosphatidylinositol mannosides from Mycobacterium bovis bacillus Calmette‑Guerin and adjuvant activity in vivo. (who.int)
Bacteria5
- Mycobacterium smegmatis possesses two such transporters, the widely distributed PstSCAB system and PhnDCE, a transporter that in other bacteria mediates the uptake of alternative phosphorus sources. (nih.gov)
- Molecular techniques have been at the forefront of the methods for studying bacteria, and the mycobacteria are no exception. (asm.org)
- These problems partially explain why the development of genetic systems for mycobacteria has lagged behind the development of genetic systems for other bacteria. (asm.org)
- Mycobacterium smegmatis are aerobic nonmotile bacteria, which are characteristically acid fast and grow well as biofilms (6). (asmscience.org)
- We performed co-culture experiments using marine-derived Aspergillus niger with Mycobacterium smegmatis, a mycolic acid-containing bacteria. (olmdiagnostics.com)
Protein9
- inhA (a fatty acid elongase), rpsL (ribosomal S12 protein), gyrA (DNA gyrase), pncA (pyrazinamidase), polA (DNA polymerase I) and rpoC (RNA polymerase β subunit) of M. smegmatis . (scirp.org)
- In this study, we evaluated a recombinant vaccine prepared from non-pathogenic Mycobacterium smegmatis (rMS) that expresses a fusion of early secreted antigenic target 6-kDa antigen (ESAT6) and culture filtrate protein 10 (CFP10). (semanticscholar.org)
- IL-17A Autoantibody Induced by Recombinant Mycobacterium smegmatis Expressing Ag85A-IL-17A Fusion Protein. (semanticscholar.org)
- Interaction between FtsW and penicillin-binding protein 3 (PBP3) directs PBP3 to mid-cell, controls cell septation and mediates the formation of a trimeric complex involving FtsZ, FtsW and PBP3 in mycobacteria. (microbiologyresearch.org)
- Summary: Treatment of growing Mycobacterium smegmatis with hydroxyurea decreased the DNA/protein ratio and markedly increased the specific activities of DNA polymerase and ATP-dependent deoxyribonuclease. (microbiologyresearch.org)
- Treating M. smegmatis with nalidixic acid also decreased the DNA/protein ratio but had no effect on the activity of either enzyme. (microbiologyresearch.org)
- A similar result was obtained for M. smegmatis that overexpressed endogenous alkaline phosphatase, indicating that PhoA is not a surface protein, contrary to a previous report. (uab.edu)
- For the M. smegmatis enzyme, the Vmax for S-adenosylmethionine formation was 1.30 μmol/min/mg protein and the Km for methionine and ATP was 288 μM and 76 μM respectively. (beds.ac.uk)
- Here, the cloning, expression, purification and crystallization of this putative SCP-x protein from M. smegmatis are reported. (iisc.ac.in)
Proteins3
- Paralogous cAMP receptor proteins in Mycobacterium smegmatis show biochemical and functional divergence. (sigmaaldrich.com)
- For each proteins, the regions containing T and B epitopes were selected at the same time to look for identical epitopes on M. smegmatis based on sequence alignments. (sld.cu)
- The role of FtsZ-associated proteins in the regulation of the assembly dynamics of Mycobacterium smegmatis FtsZ is not clear. (biochemj.org)
Gene15
- X60513 M.smegmatis gltA gene for citrate synthase. (atcc.org)
- U07954 Mycobacterium smegmatis ATCC 607 16S rRNA gene, 5' untranslated region. (atcc.org)
- U87307 Mycobacterium smegmatis extracytoplasmic function alternative sigma factor (sigE) gene, complete cds. (atcc.org)
- By using the rapid-growing Mycobacterium smegmatis as a model genetic system, a gene was selected that confers low-level FQ resistance when present on a multicopy plasmid. (pnas.org)
- A new insertion element, IS 1549 , was identified serendipitously from Mycobacterium smegmatis LR222 during experiments using a vector designed to detect the excision of IS 6110 from between the promoter region and open reading frame (ORF) of an aminoglycoside phosphotransferase gene. (asm.org)
- Only strains with a 65-kDa antigen gene with a PCR-restriction fragment length polymorphism type identical to that of M. smegmatis 607 contain IS 1549 . (asm.org)
- A previous study showed antisense PNA targeting only inh A gene inhibited the growth of M . smegmatis in pure culture [9]. (scirp.org)
- In addition, all mycobacteria also contain at least one gene encoding the low-affinity phosphate transporter, Pit. (uni-muenchen.de)
- The involvement of GlgE in glycogen biosynthesis was discovered twenty years ago when the phenotype of a temperature-sensitive Mycobacterium smegmatis mutation was rescued by the glgE gene. (jic.ac.uk)
- Using a reporter gene which encodes an unstable variant of GFP, we showed that tetracycline-inducible expression occurred in M. smegmatis and that expression levels were titratable to some extent by varying the concentration of tetracycline. (asm.org)
- Using an inducible mycobacterial shuttle vector, we have identified three ORFs within an early operon of mycobacteriophage L5 which encode gene products (gp) toxic to the host M. smegmatis when expressed. (microbiologyresearch.org)
- Conditional expression of Mycobacterium smegmatis ftsZ , an essential cell division gene. (microbiologyresearch.org)
- In further work, I demonstrated that deletion of the gene cadC in M. smegmatis resulted in a severe growth defect manifested as cell lysis during growth on rich medium. (otago.ac.nz)
- We report here a new plasmid construct designated as pMSuia from pBAD-TOPO that harbors a 1.1 kb phytase gene (phyMS) from Mycobacterium smegmatis, and expression as well as characterization of the purified recombinant M. smegmatis phytase. (iium.edu.my)
- The global variations in the gene expression pattern of drug treated (½X) and laboratory evolved drug-resistant strains (2XR and 4XR) of Mycobacterium smegmatis were obtained and compared with the M. smegmatis mc2 155 (WT) strain. (ias.ac.in)
Mutants5
- Some of the Ndh mutants have a T s lethal phenotype, which indicates that Ndh is essential for the viability of M. smegmatis . (asm.org)
- The growth rate of M. smegmatis dropped drastically with its porin-mediated OM permeability in contrast to porin mutants of Escherichia coli. (sigmaaldrich.com)
- The ERG- and MSH-deficient double mutant was significantly more sensitive to peroxide than either of the single mutants lacking either ERG or MSH, suggesting that both thiols play a role in protecting M. smegmatis against oxidative stress and that ERG is able to partly compensate for the loss of MSH. (asm.org)
- Mycobacterium smegmatis alr insertion mutants are not dependent on d-alanine for growth and display a metabolic pattern consistent with an alternative pathway for d-alanine biosynthesis. (unl.edu)
- Inductively coupled plasma mass spectroscopy (ICP-MS) analysis of wild-type M. smegmatis and energy-driven ion transport mutants revealed the molecular inventory of cations in M. smegmatis under normoxia and hypoxia. (otago.ac.nz)
Recombinant Mycobacte2
- Cellular and humoral immunogenicity of recombinant Mycobacterium smegmatis expressing Ag85B epitopes in mice. (semanticscholar.org)
- Objective To observe humoral and cellular immune responses induced in mice by a TSOL18 recombinant Mycobacterium smegmatis (MS) vaccine of Taenia solium . (bvsalud.org)
Drug resistant strains1
- Truncated structural variants of lipoarabinomannan in ethambutol drug-resistant strains of Mycobacterium smegmatis. (semanticscholar.org)
Mutation3
- Mycobacterium smegmatis is a bacterium that is naturally devoid of known postreplicative DNA mismatch repair (MMR) homologs, mutS and mutL , providing an opportunity to investigate how the mutation rate and spectrum has evolved in the absence of a highly conserved primary repair pathway. (g3journal.org)
- We also found that the transition-mutation rate of M. smegmatis is significantly lower than that of other naturally MMR-devoid or MMR-knockout organisms. (g3journal.org)
- The mutation features of M. smegmatis provide further evidence that genomes harbor alternative routes for improving replication fidelity, even in the absence of major repair pathways. (g3journal.org)
Pathogens1
- M. smegmatis and M. avium are both opportunistic pathogens (8). (asmscience.org)
Characterization2
- Our results suggest that M. smegmatis can serve as a tractable model for further characterization of the molecular mechanism of phosphate sensing in mycobacteria and to screen for compounds that would interfere with signal transduction and thereby increase the efficacy of existing anti-tubercular antibiotics. (frontiersin.org)
- expression in Mycobacterium smegmatis and biochemical characterization. (microbiologyresearch.org)
Inhibition3
- Moreover, the inhibition of phospholipase C and phospholipase A 2 activities, involved in PAF C-16 signaling pathway, increased survival of intracellular M. smegmatis . (frontiersin.org)
- Use of a number of PAF C-16 structural analogs, including Lyso-PAF, 2-O-methyl PAF, PAF C-18 and Hexanolamino PAF, revealed that the presence of acetyl group (CH 3 CO) at sn -2 position of the glycerol backbone of PAF is important for the intracellular growth inhibition activity against M. smegmatis . (frontiersin.org)
- No growth inhibition of M. smegmatis was seen in pure culture when treated with PNAs targeting gyrA and a mismatch PNA targeting dnaG (DNA primase). (scirp.org)
Pathogenic mycobacteria6
- Non-mutagenic epigallocatechin gallate inhibits the growth of M. smegmatis and warrants further investigation as an adjunct therapy for pathogenic mycobacteria. (ijpsonline.com)
- Indeed, PI-LAM induces high levels of apoptosis and IL-12 expression compared to the mannosyl modification of LAM isolated from facultative-pathogenic mycobacteria. (www.gov.uk)
- Consistently, BALB/c BMDM responded by secreting large amounts of TNF upon infection with non-pathogenic but not facultative-pathogenic mycobacteria. (www.gov.uk)
- Interestingly, C57Bl/6 BMDM do not undergo apoptosis upon infection with non-pathogenic mycobacteria despite the fact that they still induce an increase in TNF secretion. (www.gov.uk)
- These results demonstrate a much stronger induction of the innate immune response by non-pathogenic versus facultative-pathogenic mycobacteria as measured by host cell apoptosis, IL-12 and TNF cytokine induction. (www.gov.uk)
- Briken, V. The Non-Pathogenic Mycobacteria M. Smegmatis and M. Fortuitum Induce Rapid Host Cell Apoptosis Via a Caspase-3 and TNF Dependent Pathway. (www.gov.uk)
Biosynthesis1
- Impairment of D-alanine biosynthesis in Mycobacterium smegmatis " by Ofelia Barletta-Chacon, Luiz E. Bermudez et al. (unl.edu)
Saprophyte2
- In the soil dwelling saprophyte Mycobacterium smegmatis the OmpR-type response regulator GlnR is thought to mediate the transcriptomic response to nitrogen limitation. (biomedcentral.com)
- The overarching aim of this thesis was to characterise ion homeostasis and energetics in the saprophyte Mycobacterium smegmatis, under normoxia and hypoxia, under ion chelation and deficiency, with heavy-metal exposure and in response to anti-tubercular compounds with putative disruptive effects on ion energetics, including bedaquiline and amiloride derivatives. (otago.ac.nz)
Mutant11
- However, a complex plasmid swapping strategy was required to generate the Δ phoU1 Δ phoU2 mutant, suggesting that either phoU1 or phoU2 is essential for in vitro growth of M. smegmatis . (frontiersin.org)
- Deletion of egtD from wild-type M. smegmatis and an MSH-deficient mutant did not affect their susceptibility to antibiotics tested in this study. (asm.org)
- An unmarked deletion mutant in pitA of M. smegmatis was created. (uni-muenchen.de)
- However, increased expression of the remaining phosphate transporters in the mutant indicates a compensatory mechanism and implies that PitA is indeed used for the uptake of phosphate in M. smegmatis. (uni-muenchen.de)
- We investigated the role of GlnR in the nitrogen limitation response and determined the entire GlnR regulon , by combining expression profiling of M. smegmatis wild type and glnR deletion mutant, with GlnR-specific chromatin immunoprecipitation and high throughput sequencing. (biomedcentral.com)
- In this study, we demonstrate that the M. smegmatis alr insertion mutant TAM23 can synthesize d-alanine at lower levels than the parental strain. (unl.edu)
- Here we show that Mycobacterium smegmatis MmpL3 mutant strains had an altered cell wall hydrophobicity, disrupted membrane potential and growth defects in liquid media. (microbiologyresearch.org)
- M. smegmatis MmpL3 mutant strains were resistant to two anti-tubercular agents, SQ109 and AU1235, but were more sensitive to rifampicin, erythromycin and ampicillin. (microbiologyresearch.org)
- Disruption of potassium homeostasis in the ΔtrkA mutant of M. smegmatis resulted in the reduction of (~10-50%) intracellular potassium concentrations and elevated (~10-30%) intracellular sodium concentrations, coupled with reductions in viability and persistence under hypoxia. (otago.ac.nz)
- My data suggest that a ∆cadC mutant is defective in the generation or replenishment of intracellular lysine and DAP levels that are essential for growth and survival in mycobacteria. (otago.ac.nz)
- The uptake of phosphate by a mutant lacking the porins MspA and MspC was twofold lower than that by wild-type M. smegmatis. (uab.edu)
Genus1
- M. smegmatis is commonly used in work on the Mycobacterium genus due to it being a "fast grower" and non-pathogenic. (wikipedia.org)
Mycobacterial5
- The M. smegmatis strain is now the work-horse of mycobacterial genetics. (redorbit.com)
- However, in contrast to the well-known E. coli Hfr conjugation system, in M. smegmatis all regions of the chromosome are transferred with comparable efficiencies and mycobacterial conjugation is chromosome, rather than plasmid based. (wikipedia.org)
- Background: Mycobacteria have been shown to contain an apparent redundancy of high-affinity phosphate uptake systems, with two to four copies of such systems encoded in all mycobacterial genomes sequenced to date. (uni-muenchen.de)
- Unlike other bacterial biofilms which synthesize EPS composed predominantly of polysaccharides, mycobacterial EPS contains large amounts of mycolic acids (waxes), which are also found in the cell envelope of mycobacteria. (asmscience.org)
- Furthermore, it is shown that even though mycobacterial UDG-Ugi complexes dissociate in 5-6 M urea, Ugi is still a potent inhibitor of UDG activity in mycobacteria. (iisc.ernet.in)
Deletion1
- The deletion did not affect in vitro growth or phosphate uptake of M. smegmatis. (uni-muenchen.de)
Genetic2
- used genetic manipulation and microscopy to study how mycobacteria build their cell wall. (elifesciences.org)
- Superinfection immunity of mycobacteriophage L5: applications for genetic transformation of mycobacteria. (microbiologyresearch.org)
Pathway5
- The HR pathway of M. smegmatis is the major determinant of resistance to ionizing radiation and oxidative DNA damage. (wikipedia.org)
- NHEJ is the preferred pathway during stationary phase, and it protects M. smegmatis against the harmful effects of desiccation. (wikipedia.org)
- Arachidonic acid, a downstream metabolite of PAF C-16 signaling pathway, directly inhibited the growth of M. smegmatis in vitro . (frontiersin.org)
- Background - The bacterial GlgE pathway is the third known route to glycogen and is the only one present in mycobacteria. (jic.ac.uk)
- General Significance - This study highlights how the lowering of flux through the GlgE pathway can slow the growth mycobacteria. (jic.ac.uk)
Metabolism4
- Nitrogen metabolism in Mycobacterium smegmatis (Thesis, Doctor of Philosophy). (otago.ac.nz)
- In this thesis, I have developed a nitrogen-limited continuous culture system to gain further insights into nitrogen metabolism in mycobacteria and their response to nitrogen limitation. (otago.ac.nz)
- I conclude that M. smegmatis has a broad network of regulatory systems that together enable M. smegmatis to adapt its nitrogen metabolism to rapidly changing environments. (otago.ac.nz)
- However, in mycobacteria, knowledge of zinc metabolism is incomplete. (asm.org)
Nonpathogenic1
- Consequently a faster growing, nonpathogenic Mycobacterium would be ideal as a first screen in drug discovery. (bl.uk)
Enzyme3
- Growth of M. smegmatis under conditions of zinc limitation did not diminish the specific activity of DNA polymerase thus not favouring a role for zinc in the action of this enzyme. (microbiologyresearch.org)
- The kinetic properties of the M. smegmatis enzyme were also similar to known prokaryotic methionine adenosyltransferases. (beds.ac.uk)
- Ni-NTA affinity purified recombinant M. smegmatis phytase exhibited specific activity of 233.51 U/mg, optimal pH of 3 and 7 and optimal temperature of 60˚C. The purified enzyme retains almost 30% of the initial activity after incubation at 90˚C for 60 min. (iium.edu.my)
Mutations2
- Here, by infecting THP-1 macrophages with Ms_Rv2820c-Bj, Ms_Rv2820c and MS_Rv2820c-Tr (expressing the truncated Rv2820c without five amino acid mutations at 3′-end), we found only Ms_Rv2820c-Bj was responsible for the enhancement of survival of M. smegmatis in macrophages. (deepdyve.com)
- Interrupted coding sequences in Mycobacterium smegmatis : authentic mutations or sequencing errors? (biomedcentral.com)
High-affinity3
- Addgene: The Phn system of Mycobacterium smegmatis: a second high-affinity ABC-transporter for phosphate. (addgene.org)
- Furthermore, I showed that M. smegmatis has a high-affinity lysine uptake system that exhibited high rates of lysine transport during growth in minimal medium, which was significantly reduced during growth in rich medium. (otago.ac.nz)
- The present study was directed at investigating the role of the low-affinity phosphate transporter in a bacterium containing at least two high-affinity systems, using the model of M. smegmatis . (biomedcentral.com)
Pathways4
- M. smegmatis relies on DNA repair pathways to resist DNA damage. (wikipedia.org)
- The M. smegmatis PrrAB two-component system regulates respiratory and oxidative phosphorylation pathways, potentially to provide tolerance against the dynamic environmental conditions experienced in its natural ecological niche. (biomedcentral.com)
- Mycobacteria are part of the native microflora found in soils and aquatic environments and harbour an extensive repertoire of uptake and efflux and secretion pathways to modulate ions and metabolites including, sodium, potassium, calcium, magnesium, iron, nickel, manganese, zinc, copper and cobalt. (otago.ac.nz)
- There remains a paucity of information regarding ion homeostasis networks and pathways in mycobacteria. (otago.ac.nz)
Growth7
- The growth rate of Mycobacterium smegmatis depends on sufficient porin-mediated influx of nutrients. (sigmaaldrich.com)
- These results show that porin-mediated influx of nutrients is a major determinant of the growth rate of M. smegmatis. (sigmaaldrich.com)
- Here, we have examined the effect of PAF C-16 on M. smegmatis residing within macrophages, and identified mechanisms involved in their growth inhibitory function. (frontiersin.org)
- Taken together, these results suggest that exogenous PAF C-16 treatment inhibits intracellular M. smegmatis growth, at least partially, in a nitric oxide and TNF-α dependent manner. (frontiersin.org)
- A study was carried out to determine the efficacy of several antisense PNAs in inhibiting extracellular and intracellular growth of Mycobacterium smegmatis . (scirp.org)
- In this study, quantification of ERG at various points in the growth cycle of Mycobacterium smegmatis revealed that a significant portion of ERG is found in the culture media, suggesting that it is actively secreted. (asm.org)
- Strikingly, the loss of these porins resulted in a severe growth defect of M. smegmatis on low-phosphate plates. (uab.edu)
Macrophages1
- Also, the biological significance of the interaction between RvErp and Rv2212 was demonstrated by the enhanced survival of M. smegmatis within THP-1 macrophages. (osdd.net)
Resistance3
- Strains of M. smegmatis that have particularly efficient DNA repair machinery, as indicated by their greater resistance to the DNA damaging effects of agents such as UV and mitomycin C, proved to be the most capable of undergoing transformation. (wikipedia.org)
- In this report, we describe a new mechanism for INH resistance in Mycobacterium smegmatis . (asm.org)
- Overexpression of nudC, as well as inhA which encodes a known target of isoniazid, increased M. smegmatis resistance to isoniazid, but failed to increase resistance to three of the analogues, NSC27607, NSC33759, and NSC40350. (nih.gov)
Strain1
- Conjugal DNA transfer in M. smegmatis requires stable and extended contact between a donor and a recipient strain, is DNase resistant, and the transferred DNA is incorporated into the recipient's chromosome by homologous recombination. (wikipedia.org)
Biochemical1
- A detailed biochemical analysis of PhnF binding to its identified binding sites in the phnD-phnF intergenic region of M. smegmatis has allowed us to propose a quantitative model for repressor binding, which shows that a PhnF dimer binds independently to each site. (nih.gov)
Phosphate Transport1
- Collectively, our data demonstrate that the two M. smegmatis PhoU orthologs have overlapping functions in both controlling SenX3-RegX3 activity in response to phosphate availability and regulating phosphate transport by the Pst system. (frontiersin.org)
Essential3
- The specialized secretory apparatus ESX-1 is essential for DNA transfer in Mycobacterium smegmatis. (nih.gov)
- This also indicated that a minimal porin-mediated OM permeability is essential for survival of M. smegmatis. (sigmaaldrich.com)
- This study has demonstrated the molecular inventory of intracellular ions in M. smegmatis under several key laboratory conditions and has characterised essential components of ion homeostasis and more specifically, potassium homeostasis and energetics in mycobacteria. (otago.ac.nz)
Characterize1
- This study explores the potential use of computational models to characterize the sites targeted by VapC in Mycobacterium smegmatis. (waikato.ac.nz)
Inhibitory effects1
- Epigallocatechin gallate, a green tea catechin with significant antimicrobial properties, and its derivatives have been studied for their inhibitory effects on Mycobacterium smegmatis . (ijpsonline.com)
Mechanism1
- Conjugal DNA transfer in Mycobacterium smegmatis occurs by a mechanism distinct from plasmid-mediated DNA transfer. (nih.gov)
Bacterial1
- SmtB and Zur are important antagonistically acting bacterial regulators in mycobacteria. (asm.org)
Widely1
- To date, the acetamide-inducible system of Mycobacterium smegmatis has been the most widely used controllable promoter in mycobacteria ( 7 , 20 ). (asm.org)
Survival3
- When wild-type M. smegmatis were delivered to immunocompetent C57Bl/6 mice bearing the transplantable MB49 bladder tumour, efficacy comparable to live BCG was observed with 10-20% long-term survival. (nih.gov)
- Recombinant M. smegmatis secreting TNFalpha, however, gave a 70% durable tumour-free survival. (nih.gov)
- Accordingly, we concluded that the new 3′-end of Rv2820c-Bj was important to dampen host defense and enhance the intracellular survival of M. smegmatis. (deepdyve.com)
Actinobacteria1
- Mycobacterium smegmatis ist eine Art in der Gattung Mycobacterium in der Familie der Mycobacteriaceae , die zur Abteilung Actinobacteria gezählt wird. (eol.org)