A rapid-growing, nonphotochromogenic species of MYCOBACTERIUM originally isolated from human smegma and found also in soil and water. (From Dorland, 28th ed)
A genus of gram-positive, aerobic bacteria. Most species are free-living in soil and water, but the major habitat for some is the diseased tissue of warm-blooded hosts.
A species of gram-positive, aerobic bacteria that produces TUBERCULOSIS in humans, other primates, CATTLE; DOGS; and some other animals which have contact with humans. Growth tends to be in serpentine, cordlike masses in which the bacilli show a parallel orientation.
The bovine variety of the tubercle bacillus. It is called also Mycobacterium tuberculosis var. bovis.
A bacterium causing tuberculosis in domestic fowl and other birds. In pigs, it may cause localized and sometimes disseminated disease. The organism occurs occasionally in sheep and cattle. It should be distinguished from the M. avium complex, which infects primarily humans.
Infections with bacteria of the genus MYCOBACTERIUM.
So-called atypical species of the genus MYCOBACTERIUM that do not cause tuberculosis. They are also called tuberculoid bacilli, i.e.: M. buruli, M. chelonae, M. duvalii, M. flavescens, M. fortuitum, M. gilvum, M. gordonae, M. intracellulare (see MYCOBACTERIUM AVIUM COMPLEX;), M. kansasii, M. marinum, M. obuense, M. scrofulaceum, M. szulgai, M. terrae, M. ulcerans, M. xenopi.
Viruses whose host is one or more Mycobacterium species. They include both temperate and virulent types.
Infections with nontuberculous mycobacteria (atypical mycobacteria): M. kansasii, M. marinum, M. scrofulaceum, M. flavescens, M. gordonae, M. obuense, M. gilvum, M. duvali, M. szulgai, M. intracellulare (see MYCOBACTERIUM AVIUM COMPLEX;), M. xenopi (littorale), M. ulcerans, M. buruli, M. terrae, M. fortuitum (minetti, giae), M. chelonae.
A species of gram-positive, aerobic bacteria that causes LEPROSY in man. Its organisms are generally arranged in clumps, rounded masses, or in groups of bacilli side by side.
Proteins found in any species of bacterium.
A rapid-growing, nonphotochromogenic species that is potentially pathogenic, producing lesions of lung, bone, or soft tissue following trauma. It has been found in soil and in injection sites of humans, cattle, and cold-blooded animals. (Dorland, 28th ed)
A complex that includes several strains of M. avium. M. intracellulare is not easily distinguished from M. avium and therefore is included in the complex. These organisms are most frequently found in pulmonary secretions from persons with a tuberculous-like mycobacteriosis. Strains of this complex have also been associated with childhood lymphadenitis and AIDS; M. avium alone causes tuberculosis in a variety of birds and other animals, including pigs.
A species of gram-positive, aerobic bacteria commonly found in soil and occasionally isolated from sputum. It causes postoperative wound infections as well as gluteal abscesses.
Drugs used in the treatment of tuberculosis. They are divided into two main classes: "first-line" agents, those with the greatest efficacy and acceptable degrees of toxicity used successfully in the great majority of cases; and "second-line" drugs used in drug-resistant cases or those in which some other patient-related condition has compromised the effectiveness of primary therapy.
An antitubercular agent that inhibits the transfer of mycolic acids into the cell wall of the tubercle bacillus. It may also inhibit the synthesis of spermidine in mycobacteria. The action is usually bactericidal, and the drug can penetrate human cell membranes to exert its lethal effect. (From Smith and Reynard, Textbook of Pharmacology, 1992, p863)
A saprophytic bacterium widely distributed in soil and dust and on plants.
A moderate-growing, photochromogenic species found in aquariums, diseased fish, and swimming pools. It is the cause of cutaneous lesions and granulomas (swimming pool granuloma) in humans. (Dorland, 28th ed)
Any of the infectious diseases of man and other animals caused by species of MYCOBACTERIUM.
A slow-growing, photochromogenic species that is the etiologic agent of a tuberculosis-like disease in humans and is frequently isolated from human pulmonary secretions or tubercles. The incidence of infection is sharply increased among immunocompromised individuals. (Dorland, 28th ed)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A subspecies of gram-positive, aerobic bacteria. It is the etiologic agent of Johne's disease (PARATUBERCULOSIS), a chronic GASTROENTERITIS in RUMINANTS.
Antibacterial agent used primarily as a tuberculostatic. It remains the treatment of choice for tuberculosis.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
The functional hereditary units of BACTERIA.
Polysaccharides composed of repeating galactose units. They can consist of branched or unbranched chains in any linkages.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
A nontuberculous infection when occurring in humans. It is characterized by pulmonary disease, lymphadenitis in children, and systemic disease in AIDS patients. Mycobacterium avium-intracellulare infection of birds and swine results in tuberculosis.
A strongly basic peptide, antibiotic complex from several strains of Streptomyces. It is allergenic and toxic to kidneys and the labyrinth. Viomycin is used in tuberculosis as several different salts and in combination with other agents.
A slow-growing mycobacterium that infects the skin and subcutaneous tissues, giving rise to indolent BURULI ULCER.
The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.
Proteins which contain carbohydrate groups attached covalently to the polypeptide chain. The protein moiety is the predominant group with the carbohydrate making up only a small percentage of the total weight.
A semisynthetic antibiotic produced from Streptomyces mediterranei. It has a broad antibacterial spectrum, including activity against several forms of Mycobacterium. In susceptible organisms it inhibits DNA-dependent RNA polymerase activity by forming a stable complex with the enzyme. It thus suppresses the initiation of RNA synthesis. Rifampin is bactericidal, and acts on both intracellular and extracellular organisms. (From Gilman et al., Goodman and Gilman's The Pharmacological Basis of Therapeutics, 9th ed, p1160)
Substances elaborated by bacteria that have antigenic activity.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A naturally occurring metabolite of HISTIDINE that has antioxidant properties.
Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).
A second-line antitubercular agent that inhibits mycolic acid synthesis.
MYCOBACTERIUM infections of the lung.
Any compound containing one or more monosaccharide residues bound by a glycosidic linkage to a hydrophobic moiety such as an acylglycerol (see GLYCERIDES), a sphingoid, a ceramide (CERAMIDES) (N-acylsphingoid) or a prenyl phosphate. (From IUPAC's webpage)
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A pyridoxal-phosphate protein that reversibly catalyzes the conversion of L-alanine to D-alanine. EC 5.1.1.1.
An isomer of glucose that has traditionally been considered to be a B vitamin although it has an uncertain status as a vitamin and a deficiency syndrome has not been identified in man. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1379) Inositol phospholipids are important in signal transduction.
Toxic glycolipids composed of trehalose dimycolate derivatives. They are produced by MYCOBACTERIUM TUBERCULOSIS and other species of MYCOBACTERIUM. They induce cellular dysfunction in animals.
The ability of bacteria to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
Antibiotic substance produced by Streptomyces garyphalus.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Sorbitan mono-9-octadecanoate poly(oxy-1,2-ethanediyl) derivatives; complex mixtures of polyoxyethylene ethers used as emulsifiers or dispersing agents in pharmaceuticals.
Ability of a microbe to survive under given conditions. This can also be related to a colony's ability to replicate.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
Cyclic peptide antibiotic similar to VIOMYCIN. It is produced by Streptomyces capreolus.
A methylpentose whose L- isomer is found naturally in many plant glycosides and some gram-negative bacterial lipopolysaccharides.
The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
Mannosides formed by the reaction of the hydroxyl group on the anomeric carbon atom of mannose with methyl alcohol. They include both alpha- and beta-methylmannosides.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
Substances that reduce the growth or reproduction of BACTERIA.
An antibiotic produced by the soil actinomycete Streptomyces griseus. It acts by inhibiting the initiation and elongation processes during protein synthesis.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Vaccines or candidate vaccines used to prevent or treat TUBERCULOSIS.
The etiologic agent of rat leprosy, also known as murine leprosy.
A non-tuberculous mycobacterium causing cervical lymphadenitis in children. It very rarely causes pulmonary disease, and is believed to be non-pathogenic in animals.
Substances obtained from various species of microorganisms that are, alone or in combination with other agents, of use in treating various forms of tuberculosis; most of these agents are merely bacteriostatic, induce resistance in the organisms, and may be toxic.
An active immunizing agent and a viable avirulent attenuated strain of Mycobacterium tuberculosis, var. bovis, which confers immunity to mycobacterial infections. It is used also in immunotherapy of neoplasms due to its stimulation of antibodies and non-specific immunity.
Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
Oligosaccharides containing two monosaccharide units linked by a glycosidic bond.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
A family of gram-positive bacteria found in soil and dairy products and as parasites on animals and man. Several are important pathogens.
Techniques used in studying bacteria.
A slow-growing, scotochromogenic species occurring usually harmlessly in human secretions but occasionally associated with chronic pulmonary disease. (Dorland, 28th ed)
A chronic GASTROENTERITIS in RUMINANTS caused by MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS.
A chronic granulomatous infection caused by MYCOBACTERIUM LEPRAE. The granulomatous lesions are manifested in the skin, the mucous membranes, and the peripheral nerves. Two polar or principal types are lepromatous and tuberculoid.
Porins are protein molecules that were originally found in the outer membrane of GRAM-NEGATIVE BACTERIA and that form multi-meric channels for the passive DIFFUSION of WATER; IONS; or other small molecules. Porins are present in bacterial CELL WALLS, as well as in plant, fungal, mammalian and other vertebrate CELL MEMBRANES and MITOCHONDRIAL MEMBRANES.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Glycosides formed by the reaction of the hydroxyl group on the anomeric carbon atom of mannose with an alcohol to form an acetal. They include both alpha- and beta-mannosides.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Five-membered heterocyclic ring structures containing an oxygen in the 1-position and a nitrogen in the 3-position, in distinction from ISOXAZOLES where they are at the 1,2 positions.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
A semi-synthetic aminoglycoside antibiotic that is used in the treatment of TUBERCULOSIS.
The genetic complement of a BACTERIA as represented in its DNA.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Those genes found in an organism which are necessary for its viability and normal function.
The heritable modification of the properties of a competent bacterium by naked DNA from another source. The uptake of naked DNA is a naturally occuring phenomenon in some bacteria. It is often used as a GENE TRANSFER TECHNIQUE.
An infection of cattle caused by MYCOBACTERIUM BOVIS. It is transmissible to man and other animals.
Substances that suppress Mycobacterium leprae, ameliorate the clinical manifestations of leprosy, and/or reduce the incidence and severity of leprous reactions.
Substances that prevent infectious agents or organisms from spreading or kill infectious agents in order to prevent the spread of infection.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Enzymes that catalyze the transfer of mannose from a nucleoside diphosphate mannose to an acceptor molecule which is frequently another carbohydrate. The group includes EC 2.4.1.32, EC 2.4.1.48, EC 2.4.1.54, and EC 2.4.1.57.
An NAD-dependent enzyme that catalyzes the reversible DEAMINATION of L-ALANINE to PYRUVATE and AMMONIA. The enzyme is needed for growth when ALANINE is the sole CARBON or NITROGEN source. It may also play a role in CELL WALL synthesis because L-ALANINE is an important constituent of the PEPTIDOGLYCAN layer.
An enzyme that catalyzes the hydrolysis of nicotinamide to nicotinate and ammonia. EC 3.5.1.19.
A species of gram-positive, aerobic bacteria that causes granulomatous or ulcerating skin lesions in immunosuppressed persons. This organism owes its name to its requirement for growth of high levels of iron, conveniently supplied as blood, heme, or ferric ammonium citrate.
Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.
A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).
Enzymes from the transferase class that catalyze the transfer of acyl groups from donor to acceptor, forming either esters or amides. (From Enzyme Nomenclature 1992) EC 2.3.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
A pyrazine that is used therapeutically as an antitubercular agent.
A bacterial DNA topoisomerase II that catalyzes ATP-dependent breakage of both strands of DNA, passage of the unbroken strands through the breaks, and rejoining of the broken strands. Gyrase binds to DNA as a heterotetramer consisting of two A and two B subunits. In the presence of ATP, gyrase is able to convert the relaxed circular DNA duplex into a superhelix. In the absence of ATP, supercoiled DNA is relaxed by DNA gyrase.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Nonsusceptibility of bacteria to the antibiotic KANAMYCIN, which can bind to their 70S ribosomes and cause misreading of messenger RNA.
Material coughed up from the lungs and expectorated via the mouth. It contains MUCUS, cellular debris, and microorganisms. It may also contain blood or pus.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
An oxidoreductase that catalyzes the oxidative DEAMINATION of GLYCINE to glyoxylate and AMMONIA in the presence of NAD. In BACTERIA lacking transaminating pathways the enzyme can act in the reverse direction to synthesize glycine from glyoxylate and ammonia and NADH.
Highly toxic compound which can cause skin irritation and sensitization. It is used in manufacture of azo dyes.
Membrane-bound cytoplasmic vesicles formed by invagination of phagocytized material. They fuse with lysosomes to form phagolysosomes in which the hydrolytic enzymes of the lysosome digest the phagocytized material.
A hexose or fermentable monosaccharide and isomer of glucose from manna, the ash Fraxinus ornus and related plants. (From Grant & Hackh's Chemical Dictionary, 5th ed & Random House Unabridged Dictionary, 2d ed)
Techniques to alter a gene sequence that result in an inactivated gene, or one in which the expression can be inactivated at a chosen time during development to study the loss of function of a gene.
Burrowing, chiefly nocturnal mammals of the family Dasypodidae having bodies and heads encased in small bony plates. They are widely distributed in the warmer parts of the Americas.
Low-molecular-weight compounds produced by microorganisms that aid in the transport and sequestration of ferric iron. (The Encyclopedia of Molecular Biology, 1994)
Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.
Proteins prepared by recombinant DNA technology.
A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.
Enzymes that catalyze the synthesis of FATTY ACIDS from acetyl-CoA and malonyl-CoA derivatives.
Tuberculosis resistant to chemotherapy with two or more ANTITUBERCULAR AGENTS, including at least ISONIAZID and RIFAMPICIN. The problem of resistance is particularly troublesome in tuberculous OPPORTUNISTIC INFECTIONS associated with HIV INFECTIONS. It requires the use of second line drugs which are more toxic than the first line regimens. TB with isolates that have developed further resistance to at least three of the six classes of second line drugs is defined as EXTENSIVELY DRUG-RESISTANT TUBERCULOSIS.
The rate dynamics in chemical or physical systems.
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
Enzymes which reduce nitro groups (NITRO COMPOUNDS) and other nitrogenous compounds.

RecA-Mediated gene conversion and aminoglycoside resistance in strains heterozygous for rRNA. (1/838)

Clinical resistance to aminoglycosides in general is due to enzymatic drug modification. Mutational alterations of the small ribosomal subunit rRNA have recently been found to mediate acquired resistance in bacterial pathogens in vivo. In this study we investigated the effect of 16S rRNA heterozygosity (wild-type [wt] and mutant [mut] operons at position 1408 [1408wt/1408mut]) on aminoglycoside resistance. Using an integrative vector, we introduced a single copy of a mutated rRNA operon (1408 A-->G) into Mycobacterium smegmatis, which carries two chromosomal wild-type rRNA operons; the resultant transformants exhibited an aminoglycoside-sensitive phenotype. In contrast, introduction of the mutated rRNA operon into an M. smegmatis rrnB knockout strain carrying a single functional chromosomal wild-type rRNA operon resulted in aminoglycoside-resistant transformants. Subsequent analysis by DNA sequencing and RNase protection assays unexpectedly demonstrated a homozygous mutant genotype, rRNAmut/rRNAmut, in the resistant transformants. To investigate whether RecA-mediated gene conversion was responsible for the aminoglycoside-resistant phenotype in the rRNAwt/rRNAmut strains, recA mutant strains were generated by allelic exchange techniques. Transformation of the recA rrnB M. smegmatis mutant strains with an integrative vector expressing a mutated rRNA operon (Escherichia coli position 1408 A-->G) resulted in transformants with an aminoglycoside-sensitive phenotype. Subsequent analysis showed stable heterozygosity at 16S rRNA position 1408 with a single wild-type allele and a single resistant allele. These results demonstrate that rRNA-mediated mutational resistance to aminoglycosides is recessive.  (+info)

Genetic evidence that InhA of Mycobacterium smegmatis is a target for triclosan. (2/838)

Three Mycobacterium smegmatis mutants selected for resistance to triclosan each had a different mutation in InhA, an enoyl reductase involved in fatty acid synthesis. Two expressed some isoniazid resistance. A mutation originally selected on isoniazid also mediated triclosan resistance, as did the wild-type inhA gene on a multicopy plasmid. Replacement of the mutant chromosomal inhA genes with wild-type inhA eliminated resistance. These results suggest that M. smegmatis InhA, like its Escherichia coli homolog FabI, is a target for triclosan.  (+info)

Role of acid pH and deficient efflux of pyrazinoic acid in unique susceptibility of Mycobacterium tuberculosis to pyrazinamide. (3/838)

Pyrazinamide (PZA) is an important antituberculosis drug. Unlike most antibacterial agents, PZA, despite its remarkable in vivo activity, has no activity against Mycobacterium tuberculosis in vitro except at an acidic pH. M. tuberculosis is uniquely susceptible to PZA, but other mycobacteria as well as nonmycobacteria are intrinsically resistant. The role of acidic pH in PZA action and the basis for the unique PZA susceptibility of M. tuberculosis are unknown. We found that in M. tuberculosis, acidic pH enhanced the intracellular accumulation of pyrazinoic acid (POA), the active derivative of PZA, after conversion of PZA by pyrazinamidase. In contrast, at neutral or alkaline pH, POA was mainly found outside M. tuberculosis cells. PZA-resistant M. tuberculosis complex organisms did not convert PZA into POA. Unlike M. tuberculosis, intrinsically PZA-resistant M. smegmatis converted PZA into POA, but it did not accumulate POA even at an acidic pH, due to a very active POA efflux mechanism. We propose that a deficient POA efflux mechanism underlies the unique susceptibility of M. tuberculosis to PZA and that the natural PZA resistance of M. smegmatis is due to a highly active efflux pump. These findings may have implications with regard to the design of new antimycobacterial drugs.  (+info)

A mutant of Mycobacterium smegmatis defective in the biosynthesis of mycolic acids accumulates meromycolates. (4/838)

Mycolic acids are a major constituent of the mycobacterial cell wall, and they form an effective permeability barrier to protect mycobacteria from antimicrobial agents. Although the chemical structures of mycolic acids are well established, little is known on their biosynthesis. We have isolated a mycolate-deficient mutant strain of Mycobacterium smegmatis mc2-155 by chemical mutagenesis followed by screening for increased sensitivity to novobiocin. This mutant also was hypersensitive to other hydrophobic compounds such as crystal violet, rifampicin, and erythromycin. Entry of hydrophobic probes into mutant cells occurred much more rapidly than that into the wild-type cells. HPLC and TLC analysis of fatty acid composition after saponification showed that the mutant failed to synthesize full-length mycolic acids. Instead, it accumulated a series of long-chain fatty acids, which were not detected in the wild-type strain. Analysis by 1H NMR, electrospray and electron impact mass spectroscopy, and permanganate cleavage of double bonds showed that these compounds corresponded to the incomplete meromycolate chain of mycolic acids, except for the presence of a beta-hydroxyl group. This direct identification of meromycolates as precursors of mycolic acids provides a strong support for the previously proposed pathway for mycolic acid biosynthesis involving the separate synthesis of meromycolate chain and the alpha-branch of mycolic acids, followed by the joining of these two branches.  (+info)

Integron-mediated rifampin resistance in Pseudomonas aeruginosa. (5/838)

A new rifampin resistance gene, arr-2, has been found in Pseudomonas aeruginosa. The ARR-2 protein shows 54% amino acid identity to the rifampin ADP-ribosylating transferase encoded by the arr gene from Mycobacterium smegmatis. This arr-2 gene is located on a gene cassette within a class I integron.  (+info)

Apoptosis of Mycobacterium avium-infected macrophages is mediated by both tumour necrosis factor (TNF) and Fas, and involves the activation of caspases. (6/838)

Mycobacterium avium causes disseminated infection in AIDS patients and several forms of infection in immunocompetent hosts. Recent studies have shown that M. avium infection of macrophages in vitro leads to apoptosis of significant numbers of infected cells. Several strains of M. avium used to infect human macrophages for 5 days (multiplicity of infection of 10) triggered 28-46% higher levels of apoptosis than observed with uninfected macrophages at the same time points. Mycobacterium avium strains unable to replicate intracellularly (rep-) resulted in a 15% rate of apoptosis, while M. smegmatis-infected monolayers showed the same percentage of apoptotic cells as the uninfected macrophage control. The presence of anti-TNF-alpha antibody reduced apoptosis to 17% and the presence of anti-Fas antibody reduced apoptosis to 10%. When both antibodies were used together, the apoptosis level was 5% above the control. Treatment with TGF-beta also reduced the number of apoptotic cells in infected monolayers. If intracellular growth was inhibited, apoptosis of macrophages decreased significantly. It was also shown that apoptosis was associated with IL-1 beta-converting enzyme (ICE) activation and was significantly reduced by a caspase inhibitor. Gaining understanding of the mechanisms of M. avium-associated apoptosis of macrophages will provide important insight into M. avium pathogenesis.  (+info)

Enhanced gene replacement in mycobacteria. (7/838)

Allelic replacement will be a vital tool for understanding gene function in mycobacteria. Disruption of the chromosomal hisD gene of Mycobacterium smegmatis by standard gene replacement methods was surprisingly difficult, with most products being caused by illegitimate recombination (IR) events. A recombination assay was therefore developed and used to optimize conditions for homologous recombination (HR) in M. smegmatis. Treatment of competent cells with UV, hydrogen peroxide or mitomycin C did not improve the frequency of HR; however, treatment of the DNA with alkali or UV enhanced recombination frequency, while boiling did not. Applying these observations to allele replacement, UV and alkali treatment of transforming DNA increased HR events with pyrF and hisD, while the level of IR was unchanged. The introduction of ss phagemid DNA improved the level of HR and abolished IR. In Mycobacterium intracellulare the use of alkali-denatured DNA increased the numbers of recombinants obtained with an inactivated 19Ag gene, while in Mycobacterium tuberculosis, inactivation of a putative haemolysin gene, tlyA, was achieved using both UV-irradiated DNA and ss phagemid DNA. Significantly, IR, which has been reported to be a problem in this species, was not observed. Thus, four genes in three species were successfully knocked-out using non-replicating DNA pretreated with alkali, UV or in an ss form. The use of these methods to enhance HR will greatly facilitate experiments to inactivate other genes in these important species.  (+info)

Enhancing the immunotherapeutic potential of mycobacteria by transfection with tumour necrosis factor-alpha. (8/838)

In an attempt to enhance the anti-tumour properties of mycobacteria we have developed recombinant forms of Mycobacterium smegmatis which express and secrete biologically active human tumour necrosis factor-alpha (TNF-alpha). This was achieved by transfecting M. smegmatis using shuttle plasmids incorporating the cDNA sequence for the human TNF-alpha mature peptide. In vitro experiments on a panel of human bladder tumour cell lines (EJ18, MGH-U1, RT4, RT112) indicate that our genetically modified mycobacteria are more effective than wild-type at inducing or up-regulating the expression of intracellular adhesion molecule-1 and the secretion of an array of proinflammatory cytokines [interleukin-1 (IL-1), IL-6, IL-8, granulocyte-macrophage colony-stimulating factor]. We have also demonstrated increased adhesion molecule and cytokine expression in response to mycobacteria transfected with vector containing no gene insert. However, this was not as pronounced as that observed following tumour cell stimulation by the TNF-alpha-transfected strain. In contrast, in three out of four tumour cell lines all M. smegmatis strains were found to down-regulate the secretion of the anti-inflammatory cytokine transforming growth factor-beta1. Our studies have also confirmed that M. smegmatis is a powerful inhibitor of bladder tumour cell growth and revealed that its antiproliferative potency is enhanced by transfecting with human TNF-alpha and, to a lesser extent, with vector alone. All M. smegmatis strains were effective in the activation of peripheral blood leucocyte cultures. However, no differences were observed in the ability of the TNF-alpha-transfected, mock-transfected and wild-type mycobacteria to induce tumour cell killing activity. These results suggest that the immunomodulatory effects of M. smegmatis can be enhanced by transfection with vectors which allow the secretion of human TNF-alpha, thus increasing mycobacterial immunotherapeutic potential.  (+info)

FIG. 1. Developing Mycobacterium smegmatis biofilm (5 days old) in 12-well cell culture cluster plates using modified M63 liquid media. FIG. 2. Mature Mycobacterium smegmatis biofilm (7 days old) in 12-well cell culture cluster plates using modified M63 liquid media. Introduction Microorganisms have many ways to ensure their survival, such as sporulation and biofilm formation. In nature, most microbes live as communities in biofilms, a conglomeration of bacteria and other microbes embedded in a self-produced and secreted matrix of extracellular polymeric substances (EPS) (1). The EPS can be composed of polysaccharides, proteins, nucleic acids, and lipids. The biofilm functions as a protective hydrated barrier between the bacterial cells and their environment. It facilitates survival under harsh conditions and environmental insults such as ultraviolet radiation, physicochemical stresses, desiccation, and insufficient supply of nutritive resources (3). Mycobacterium smegmatis are aerobic
Wissenschaftlicher Name Mycobacterium smegmatis (Trevisan 1889) Lehmann & Neumann 1899 Mycobacterium smegmatis ist ein Bakterium aus der Gruppe der Mykobakterien. Es handelt sich um stäbchenförmige Bakterien mit einer Länge von 3 bis 5 µm.[1] Es zählt zur Normalflora des Smegmas im menschlichen Genitalbereich, also sowohl im Bereich der weiblichen Vulva und der Klitorisvorhaut, Präputium clitoridis, als auch beim Mann unter dem Praeputium penis. Auch in Bodenproben wurde es häufig gefunden.[2] Es ist nur in seltenen Fällen pathogen[3] und zählt zu den sogenannten nichttuberkulösen Mykobakterien (MOTT). Mycobacterium smegmatis zählt hier zu den schnellwachsenden Mykobakterien, die auf agarhaltigen Nährmedien schon innerhalb einer Woche gut sichtbare Kolonien bilden. Deshalb gilt es auch als Modellorganismus für Mykobakterien und wird oft in Labors für Stoffwechseluntersuchungen verwendet.[4] Die elektronenmikroskopische Charakterisierung erfolgte unter Einsatz der ...
Mycobacterium smegmatis is 3.0 to 5.0 µm long with a bacillus shape, an acid-fast bacterial species in the phylum Actinobacteria. It can be stained by Ziehl-Neelsen method and the auramine-rhodamine fluorescent method. It was first reported in 1884. Alvarez and Tavel found organisms similar to Lustgarten, who first discovered Mycobacterium. This organism was later named M. smegmatis. It is considered a non-pathogenic microorganism although, in rare cases, it can cause disease. M. smegmatis is useful for the research analysis of other Mycobacteria species in laboratory experiments. Since it is a fast grower and non-pathogenic it makes a simple model that is easy to work with. It shares more than 2000 homologs with M. tuberculosis and shares the same unusual cell wall structure of M. tuberculosis and other mycobacterial species. The discovery of plasmids, phages, and mobile elements has enabled the construction of dedicated gene-inactivation and gene reporter systems. The M. smegmatis strain is ...
Mycobacterium smegmatis LfrA protein: proton antiporter isolated from Mycobacterium smegmatis; amino acid sequence in first source; GenBank U40487
Members of the VapC family of proteins cleave RNA at specific sites in order to regulate biological processes with a cell. Characterization of the sites targeted by a specific protein using conventional biochemical techniques is resource intensive. This study explores the potential use of computational models to characterize the sites targeted by VapC in Mycobacterium smegmatis. Previous work has reported the impact of VapC upon each gene in the M. smegmatis genome and produced a hypothesis model for the specific motif targeted by the enzyme. However, this model has been shown to be insufficient for the differentiation of sites cleaved by VapC from those not cleaved. This study aims to extend this model to accurately describe the features which influence VapC activity at a site. A model capable of accurately predicting the VapC target sites could supplement the existing biochemical techniques. Furthermore, a process developed to train such a model could potentially be generalized and applied to ...
The Mycobacterium tuberculosis exported repetitive protein (RvErp) is a crucial virulence-associated factor as determined by its role in the survival and multiplication of mycobacteria in cultured macrophages and in vivo Although attempts have been made to understand the function of Erp protein, its exact role in Mycobacterium pathogenesis is still elusive. One way to determine this is by searching for novel interactions of RvErp. Using a yeast two-hybrid assay, an adenylyl cyclase (AC), Rv2212, was found to interact with RvErp. The interaction between RvErp and Rv2212 is direct and occurs at the endogenous level. The Erp protein of Mycobacterium smegmatis (MSMEG_6405, or MsErp) interacts neither with Rv2212 nor with Ms_4279, the M. smegmatis homologue of Rv2212. Deletion mutants of Rv2212 revealed its adenylyl cyclase domain to be responsible for the interaction. RvErp enhances Rv2212-mediated cyclic AMP (cAMP) production. Also, the biological significance of the interaction between RvErp and ...
Several bacterial species have been shown to accumulate DNA damages upon desiccation. Deinococcus radiodurans is extremely resistant to ionizing radiation. The functions necessary to survive ionizing radiation are also necessary to survive prolonged desiccation.[3] Radiation resistance is considered to be an incidental consequence of the organisms evolutionary adaptation to dehydration, a common physiological stress in nature.[3] The chromosomal DNA from desiccated D. radiodurans revealed increased DNA double-strand breaks.[4] DNA double-strand breaks are repaired principally by a RecA-dependent recombination process that requires the presence of two genome copies.[4] By this process D. radiodurans can survive thousands of double-strand breaks per cell.[4] Mycobacterium smegmatis mutant strains that are deficient in the ability to repair double-strand breaks by the non-homologous enjoining (NHEJ) pathway are more sensitive to prolonged desiccation during stationary phase than wild-type ...
bacteriaceae36 and is well conserved at the DNA se- In contrast to the plasmid-mediated antiseptic/ quence level in S. typhimurium21. Expression of disinfectant resistance of Gram-positive and Gram- E. coli MarA in Mycobacterium smegmatis mc2155 negative organisms6, E. coli mutants resistant to pine produces a mar phenotype37, suggesting that MarA oil or formulations containing this natural product are can activate cognate promoters in a heterologous mar mutants6. Inactivation of AcrAB in these mutants host, and that a mar-like system(s) exists in M. smeg- increases susceptibility to compounds containing matis. Exposure of Burkholderia (Pseudomonas)cepacia38 and Staphylococcus aureus39 to salicylate,as well as serial exposure of Pseudomonas aeruginosa40to fluoroquinolones, confers resistance to multiple antibiotics. Whether there is a chromosomal region in • Is MarR activity or expression controlled by other genes in these organisms that is homologous to the E. coli mar locus and has a similar ...
Pathogenic mycobacteria use virulence factors, including mannose-capped lipoarabinomannan (ManLAM), to survive in host phagocytic cells, such as neutrophils. We assessed the roles of lactosylceramide (LacCer, CDw17)-enriched lipid rafts in the phagocytosis of mycobacteria by human neutrophils and in the intracellular fate of phagocytosed mycobacteria. We showed that the association of the Src family kinase (SFK) Lyn with C24 fatty acid chain-containing LacCer was essential for the phagocytosis of mycobacteria by neutrophils. Assays with LacCer-containing liposomes, LacCer-coated plastic plates, and LAM-coated beads demonstrated that the phagocytosis of mycobacteria was mediated through the binding of LacCer to LAM. Both ManLAM from pathogenic species and phosphoinositol-capped LAM (PILAM) from nonpathogenic Mycobacterium smegmatis bound equivalently to LacCer to stimulate phagocytosis. However, PILAM from an M. smegmatis α1,2-mannosyltransferase deletion mutant (ΔMSMEG_4247), lacking the ...
The HIV pandemic raised the potential for facultative-pathogenic mycobacterial species like, Mycobacterium kansasii, to cause disseminating disease in humans with immune deficiencies. In contrast, non-pathogenic mycobacterial species, like M. smegmatis, are not known to cause disseminating disease even in immunocompromised individuals. We hypothesized that this difference in phenotype could be explained by the strong induction of an innate immune response by the non-pathogenic mycobacterial species.. A comparison of two rapid-growing, non-pathogenic species (M. smegmatis and M. fortuitum) with two facultative-pathogenic species (M. kansasii and M. bovis BCG) demonstrated that only the non-pathogenic bacteria induced strong apoptosis in human THP-1 cells and murine bone marrow-derived macrophages (BMDM) and dendritic cells (BMDD). The phospho-myo-inositol modification of lipoarabinomannan (PI-LAM) isolated from non-pathogenic species may be one of the cell wall components responsible for the ...
Microorganisms must maintain equilibrium and intracellular availability of ions for growth and persistence in a range of environments, either in the face of scarce resources or excesses, whereby they must buffer, exclude or export these ions preventing accumulation to harmful levels. Ion homeostasis is essential for energy generation, metabolism, regulation of internal pH and osmotic homeostasis. Mycobacteria are part of the native microflora found in soils and aquatic environments and harbour an extensive repertoire of uptake and efflux and secretion pathways to modulate ions and metabolites including, sodium, potassium, calcium, magnesium, iron, nickel, manganese, zinc, copper and cobalt. There remains a paucity of information regarding ion homeostasis networks and pathways in mycobacteria. The overarching aim of this thesis was to characterise ion homeostasis and energetics in the saprophyte Mycobacterium smegmatis, under normoxia and hypoxia, under ion chelation and deficiency, with ...
Gene target information for MSMEG_6441 - TetR family transcriptional regulator (Mycobacterium smegmatis str. MC2 155). Find diseases associated with this biological target and compounds tested against it in bioassay experiments.
The mechanism of glutathione (GSH) depletion by isoniazid (INH) was studied inM. smegmatis. INH increased the activity of γ-glutamyl transferase (GGT) whether added before medium inoculation or to actively growing cells. The activity of GGT in cells grown from the beginning in INH-containing medium increased significantly on growth days 2-6. Three-day oldM. smegmatis cells treated with INH exhibited a 30-65 % increase in the activity of GGT. The activities of γ-glutamyl-cysteine synthase (GGCS) and GSH synthase (GS) were lowered by 50 and 56 % respectively on the second day of growth whenM. smegmatis was grown in a medium supplemented with 1.5 mg INH per L. In 3-day oldM. smegmatis, INH significantly inhibited the activities of GSH biosynthetic enzymes. The results demonstrate that the increased activity of GGT and decreased activities of GSH biosynthetic enzymes are responsible for GSH depletion by INH inM. smegmatis.
Polar elongating mycobacteria (Mycobacterium smegmatis) require specific cell wall chemistries, those catalyzed by targets of critical antibiotics, to maintain rod shape at aging sites of the bacillus.
BioAssay record AID 562238 submitted by ChEMBL: Antimycobacterial activity against Mycobacterium smegmatis assessed as growth inhibition in mid-logarithmic phase by spectrophotometry.
The irreversible dodecamerization of native Dps trimers from Mycobacterium smegmatis, inVitro, is known to be directly associated with the bimodal function of this protein. Hence it is importantto explore this pathway at the molecular level. Two types of trimers, Trimer A (tA)and Trimer B (tB),can be derived from the dodecamer due to the inherent 3-fold symmetry of the spherical crystal structure.These derived trimers were expressed as protein structure graphs (PSGs) using the computed interaction strength among the residues. Interface clusters which were identified from PSGs allowed us to convincingly predict E146 and F47 for further mutation studies. Various single and double mutants were constructedand characterized. We were finally able to generate a single mutant F47 impaired in dodecamerization and a double mutant E146AF47E as native monomer in solution. These two observed results suggest that the two trimers are important for dodecamerization and that the residues selected are important ...
The recent resurgence of drug resistant strains of Mycobacterium tuberculosis, the causal agent of tuberculosis (TB) has strengthened the need for new anti-TB drugs. However, the accompanying experiments are lengthy due to the slow growth rate and pathogenic nature of the tubercle bacillus. Consequently a faster growing, nonpathogenic Mycobacterium would be ideal as a first screen in drug discovery. This project investigated metabolic features of M smegmatis to establish its utility as a first screen in the discovery of metabolic drug targets ...
Sikder, Devanjan and Unniraman, Shyam and Bhaduri, Tisha and Nagaraja, Valakunja (2001) Functional Cooperation Between Topoisomerase I and Single Strand DNA-binding Protein. In: Journal of Molecular Biology, 306 . pp. 669-679. Bhaduri, Tisha and Basak, Shashwati and Sikder, Devanjan and Nagaraja, Valakunja (2000) Inhibition of Mycobacterium smegmatis topoisomerase I by specific oligonucleotides. In: FEBS Letters, 486 (2). pp. 126-130. Bhaduri, Tisha and Baguis, Tapan Kumar and Sikder, Devanjan and Nagaraja, Valakunja (1998) DNA Topoisomerase I from Mycobacterium smegmatis - An Enzyme with Distinct Features. In: The Journal of Biological Chemistry, 273 (22). pp. 13925-13935. Bhaduri, Tisha and Nagaraja, V (1994) DNA topoisomerase I from Mycobacterium smegmatis. In: Indian Journal of Biochemistry & Biophysics, 31 . pp. 339-343. ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Background - The bacterial GlgE pathway is the third known route to glycogen and is the only one present in mycobacteria. It contributes to the virulence of…
Background - The bacterial GlgE pathway is the third known route to glycogen and is the only one present in mycobacteria. It contributes to the virulence of…
As part of an international effort and a national programme, structural analysis of mycobacterial proteins involved in recombination and repair, stringent response and protein synthesis has been undertaken, and work on proteins in a couple of metabolic pathways has been initiated. Already X-ray analysed are Mycobacterium tuberculosis and Mycobacterium smegmatis RecA and their nucleotide complexes, and different crystal forms of M. tuberculosis single-stranded DNA binding protein, M. smegmatis DNA binding protein from stationary phase cells and M. tuberculosis ribosome recycling factor. A comparative study involving these structures and those of similar proteins from other sources brings out the special features of the mycobacterial proteins, which are likely to be useful in selective inhibitor design. The structures provide insights into the plasticity of the molecules and its biological implications, and yield valuable information on their assembly and quaternary structure. They also provide ...
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. May play a role in the intracellular survival of mycobacteria.
Protected from host immune attack and antibiotic penetration by their unique cell envelope, mycobacterial pathogens cause devastating human diseases such as tuberculosis. Seamless coordination of cell growth with cell envelope elongation at the pole maintains this barrier. Unraveling this spatiotemporal regulation is a potential strategy for controlling mycobacterial infections. Our biochemical analysis previously revealed two functionally distinct membrane fractions in Mycobacterium smegmatis cell lysates: plasma membrane tightly associated with the cell wall (PM-CW) and a distinct fraction of pure membrane free of cell wall components (PMf). To provide further insight into the functions of these membrane fractions, we took the approach of comparative proteomics and identified more than 300 proteins specifically associated with the PMf, including essential enzymes involved in cell envelope synthesis such as a mannosyltransferase, Ppm1, and a galactosyltransferase, GlfT2. Furthermore, comparative
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Sigma-Aldrich offers abstracts and full-text articles by [Ritu Sharma, Anisha Zaveri, Jayashree Gopalakrishnapai, Thiruneelakantan Srinath, Srinath Thiruneelakantan, Umesh Varshney, Sandhya S Visweswariah].
expression of MsParA DsRed2 fusion proteins. A linker was placed among MsParA and DsRed2 to stop achievable difficulties with protein folding. The recombinant plasmid pMV261MsTAG GFP/MsParA DsRed2 was electroporated into M. smegmatis. The resulting recombinant M. smegmatis stains were grown in 7H9 Kan Tw media at 37uC for two d, then cultured at 42uC for 2 h to improve the level of protein expression. Next, cells were collected and visualized by vibrant area and fluorescence microscopy using a Zeiss Axio Scope A1 microscope by using a CoolSnap ES CCD camera and a substantial strain mercury lamp. The MsTAG GFP fusion proteins have been imaged employing a GFP filter and MsParA DsRed2 fusion pro teins had been imaged working with a TRITC filter .. Digital photos were acquired and analyzed together with the Image Pro Plus computer software . M. smegmatis cells Ms/pMV261, Ms/pMV261MsTAG and Ms/ pMV261 MsTAG E46A have been cultured at 37uC in 7H9 media with 0. 012% MMS, and MsParA deleted mutant ...
BACKGROUND: Mycobacterium smegmatis is fast growing non-pathogenicmycobacteria. This organism has been widely used as a model organism tostudy the biology of other virulent and extremely slow growing specieslike Mycobacterium tuberculosis. Based on the homology of the N-terminalDNA binding domain, the recently sequenced genome of M. smegmatis has beenshown to possess several putative GntR regulators. A strikingcharacteristic feature of this family of regulators is that they possess aconserved N-terminal DNA binding domain and a diverse C-terminal domaininvolved in the effector binding and/or oligomerization. Since thephysiological role of these regulators is critically dependent uponeffector binding and operator sites, we have analysed and classified theseregulators into their specific subfamilies and identified their potentialbinding sites. RESULTS: The sequence analysis of M. smegmatis putativeGntRs has revealed that FadR, HutC, MocR and the YtrA-like regulators areencoded by 45, 8, 8 and 1 ...
Peptidoglycan (PG), a polymer cross-linked by d-amino acid-containing peptides, is an essential component of the bacterial cell wall. We found that a fluorescent d-alanine analog (FDAA) incorporates chiefly at one of the two poles in Mycobacterium smegmatis but that polar dominance varies as a function of the cell cycle in Mycobacterium tuberculosis: immediately after cytokinesis, FDAAs are incorporated chiefly at one of the two poles, but just before cytokinesis, FDAAs are incorporated comparably at both. These observations suggest that mycobacterial PG-synthesizing enzymes are localized in functional compartments at the poles and septum and that the capacity for PG synthesis matures at the new pole in M ...
Our laboratory studies regulatory networks in Mycobacteria that control intrinsic resistance to a variety of stresses imposed by its environment, including oxidative and genotoxic stress, hypoxia, nutrient starvation, and exposure to multiple antibiotics which are common to both pathogenic and saprophytic species. Although a number of pathways have been described to account for the observed resistances, the mechanisms that control the expression of genes required in these processes remain poorly defined.. The ability of M. tuberculosis, the causative agent of tuberculosis, to persist for many years in its human host, and the requirement for lengthy antibiotic regimens to eliminate drug sensitive strains reflects the effectiveness of the responses to stressful environments, many of which are likely to be adapted from stress responses common to mycobacteria, including saprophytes such as Mycobacterium smegmatis, our model organism. The detailed pathways, signals, regulatory responses and molecular ...
Little is known about iron efflux transporters within bacterial systems. Recently, the participation of Bacillus subtilis PfeT, a P1B4-ATPase, in cytoplasmic Fe(2+) efflux has been proposed. We report here the distinct roles of mycobacterial P1B4-ATPases in the homeostasis of Co(2+) and Fe(2+) Mutation of Mycobacterium smegmatis ctpJ affects the homeostasis of both ions. Alternatively, an M. tuberculosis ctpJ mutant is more sensitive to Co(2+) than Fe(2+), whereas mutation of the homologous M. tuberculosis ctpD leads to Fe(2+) sensitivity but no alterations in Co(2+) homeostasis. In vitro, the three enzymes are activated by both Fe(2+) and Co(2+) and bind 1 eq of either ion at their transport site. However, equilibrium binding affinities and activity kinetics show that M. tuberculosis CtpD has higher affinity for Fe(2+) and twice the Fe(2+)-stimulated activity than the CtpJs. These parameters are paralleled by a lower activation and affinity for Co(2+) Analysis of Fe(2+) and Co(2+) binding to CtpD by x
SWISS-MODEL Repository entry for A0R025 (MRAZ_MYCS2), Transcriptional regulator MraZ. Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacteriumsmegmatis)
years ago Their partnership last year resulted in projects that allowed two Ferrum College students Terry Hall and Kevin Reynolds to present their research at a recent national meeting of the American Chemical Society It is wonderful to be able to involve students in meaningful research to benefit a local corporation and also to allow the students to present their work at technical conferences said Powell Dr Gazdik will be joining the collaboration this year bringing her expertise in microbiology to promote and monitor bacterial biofilm growth I am excited to join Dr Powell and International Scientific Technologies as they begin Phase 2 of this project It is a new line of research for me and I am looking forward to the diverse engaging and innovative research opportunities this will provide for our students said Gazdik who recently completed a project funded by the National Institute of Allergy and Infectious Diseases studying Mycobacterium smegmatis as a model organism for tuberculosis Her ...
4rs3: crystal structure of carbohydrate transporter a0qyb3 from mycobacterium smegmatis str. mc2 155, target efi-510969, in complex with xylitol
Koenzim F420 (8-hidroksi-5-deazaflavin) je koenzim koji učestvuje u redoks reakcijama u metanogenima,[1] u mnogim aktinobakterijama, i sporadično u drugim bakterijskim rodovima. On je flavinski derivat. Koenzim je supstrat za koenzim F420 hidrogenazu,[2] 5,10-metilentetrahidrometanopterin reduktazu i metilentetrahidrometanopterin dehidrogenazu.[3][4] Posebno bogat prirodni izvor F420 je Mycobacterium smegmatis, u kojoj nekoliko desetina enzima koristi F420 umesto srodnog kofaktora FMN koji koriste homologni enzimi većine drugih vrsta.[5] ...
Throughout this experiment, the phages were incubated in the M. smegmatis culture for at least 24 hours but up to 48 hours. Plaques formed by this phage in M. smegmatis culture are consistently round and clear and approximately 3-4 mm in diameter ...
Mycobacteriophage L5 is a temperate phage with a broad host range among the fast- and slow-growing mycobacteria such as Mycobacterium smegmatis, Mycobacterium tuberculosis, Mycobacterium avium and Mycobacterium ulcerans. L5 switches off host protein synthesis during the early stage of lytic growth, as was previously shown by protein expression profiling. Also, lethal genetic elements have been identified in L5 based on the fact that transformants could not be obtained with these genes. Using an inducible mycobacterial shuttle vector, we have identified three ORFs within an early operon of mycobacteriophage L5 which encode gene products (gp) toxic to the host M. smegmatis when expressed. These ORFs, coding for gp77, gp78 and gp79, presumably function as shut-off genes during early stages of phage replication. There is evidence that cell division is affected by one of the proteins (gp79). The transcription of the cytotoxic polypeptides is directed by a promoter situated in ORF83 and transcription control
TY - JOUR. T1 - Mycobacterium smegmatis L-alanine dehydrogenase (Ald) is required for proficient utilization of alanine as a sole nitrogen source and sustained anaerobic growth. AU - Feng, Zhengyu. AU - Cáceres, Nancy E.. AU - Sarath, Gautam. AU - Barletta, Raúl G.. PY - 2002/9. Y1 - 2002/9. N2 - NAD(H)-dependent L-alanine dehydrogenase (EC 1.4.1.1) (Aid) catalyzes the oxidative deamination of L-alanine and the reductive amination of pyruvate. To assess the physiological role of Aid in Mycobacterium smegmatis, we cloned the ald gene, identified its promoter, determined the protein expression levels, and analyzed the combined effects of nutrient supplementation, oxygen availability, and growth stage on enzyme activity. High Ald activities were observed in cells grown in the presence of L- or D-alanine regardless of the oxygen availability and growth stage. In exponentially growing cells under aerobic conditions, supplementation with alanine resulted in a 25- to 50-fold increase in the enzyme ...
Pathological infection caused by Mycobacterium tuberculosis is still a major global health concern. Traditional diagnostic methods are time-consuming, less sensitive, and lack high specificity. Due to an increase in the pathogenic graph of mycobacterial infections especially in developing countries, there is an urgent requirement for a rapid, low cost, and highly sensitive diagnostic method. D29 mycobacteriophage, which is capable of infecting and killing M. tuberculosis, projects itself as a potential candidate for the development of novel diagnostic methods and phage therapy of mycobacterial infections. In our previous study, we showed that the cell wall binding domain [C-terminal domain (CTD)] located at the C-terminal end of the D29 mycobacteriophage LysA endolysin very selectively binds to the peptidoglycan (PG) of Mycobacterium smegmatis and M. tuberculosis. Here, by using M. smegmatis as model organism and by exploiting the PG binding ability of CTD, we have developed a method to isolate M.
Bacteriophages, or phages, are viruses that infect bacteria. Mycobacteriophages are bacteriophages that specifically infect the genus Mycobacterium. This genus of bacteria includes human pathogens such as Mycobacterium tuberculosis, Mycobacterium leprae and Mycobacterium ulcerans, which cause tuberculosis, leprosy and Buruli ulcer, respectively. The full genome sequences of 654 mycobacteriophages are currently available. Collectively, these 654 phages encode 69,581 genes. Only 20.25% of these genes have at least one known homologue in NCBI, the National Center for Biotechnology Information, leaving roughly 80% of all known mycobacteriophage genes without even a predicted function. Bacteriophages are highly host-specific and typically only infect a small number of bacterial hosts. The host range of 204 mycobacteriophages, initially isolated on Mycobacterium smegmatis strain mc2 155, was recently determined on three other bacterial hosts: M. tuberculosis and two M. smegmatis strains, Jucho and MKD8. The
Aeras, a Rockville, MD-based non-profit development partnership dedicated to preventing TB, has licensed the technology described in this study and is using it to develop a new TB vaccine. The technology could also provide the basis for vaccines that eliminate leprosy and other virulent mycobacteria from infected tissues.. The groups paper is titled A recombinant Mycobacterium smegmatis induces potent bactericidal immunity against M. tuberculosis. Other Einstein researchers involved in the study were lead author Kari Sweeney, Ph.D.; Dee Dao, Ph.D.; Michael Goldberg, M.S.; Tsungda Hsu, Ph.D.; Manjunatha Venkataswamy, Ph.D.; Rani Sellers, Ph.D., DVM; Paras Jain, Ph.D.; Bing Chen, M.D.; Mei Chen; John Kim, Regy Lukose, John Chan, M.D.; and Steven Porcelli, M.D.. Diane Ordway, Ph.D., and Ian Orme, Ph.D., of Colorado State University, Fort Collins, CO were also co-authors of the study. The research was funded by the National Institute of Allergy and Infectious Diseases, part of the National ...
TY - JOUR. T1 - Positional isotope exchange analysis of the Mycobacterium smegmatis cysteine ligase (MshC). AU - Williams, La Kenya. AU - Fan, Fan. AU - Blanchard, John S.. AU - Raushel, Frank M.. PY - 2008/4/22. Y1 - 2008/4/22. N2 - MshC catalyzes the ATP-dependent condensation of GlcN-Ins and cysteine to form Cys-GlcN-Ins, which is an intermediate in the biosynthetic pathway of mycothiol, i.e., 1-D-myo-inosityl-2-(N-acetyl-L-cysteinyl)amido-2-deoxy-α- D-glucopyranoside (MSH or AcCys-GlcN-Ins). MSH is produced by Mycobacterium tuberculosis, members of the Actinomycetes family, to maintain an intracellular reducing environment and protect against oxidative and antibiotic induced stress. The biosynthesis of MSH is essential for cell growth, and therefore, the MSH biosynthetic enzymes present potential targets for inhibitor design. The formation of kinetically competent adenylated intermediates was suggested by the observation of positional isotope exchange (PIX) reaction using [βγ-18O6]-ATP in ...
The activity of ornithine decarboxylase in Mycobacterium smegmatis is regulated by a novel macromolecular inhibitor-a ribonucleic acid. Addition of polyamines to the growth medium enhances the level of this inhibitor, suggesting that the level of this negative modulator changes in response to the intracellular concentration of polyamines. Thus, while other modes of regulation may be operational, the control by polyamines at the transcriptional level leading to the generation of a specific RNA inhibitor seems to be a key element in the regulation of ornithine decarboxylase in mycobacteria. ...
Mycobacterium smegmatis ATCC ® 607™ Designation: TypeStrain=False Application: Assay of bleomycin Bacteriophage host Pharmaceutical and Personal Care
Mycobacterium smegmatis ATCC ® 607™ Designation: TypeStrain=False Application: Assay of bleomycin Bacteriophage host Pharmaceutical and Personal Care
Mycobacteriophage L5 gp71 protein: confers immunity to L5 superinfection; 183 amino acid residues; amino acid sequence given in first source
Tuberculosis caused by Mycobacterium tuberculosis continues to pose a serious global health threat. The attenuated Mycobacterium bovis bacillus Calmette-Guérin, as the only licensed vaccine, has limited protective efficacy against TB. The development of more effective antituberculosis vaccines is urgent and demands for further identification and understanding of M. tuberculosis Ags. MPT83 (Rv2873), a secreted mycobacterial lipoprotein, has been applied into subunit vaccine development and shown protective effects against M. tuberculosis infection in animals; however, the understanding of the underlying mechanism is limited. In present study, we systematically studied the effect of MPT83 on macrophage apoptosis by constructing Mycobacterium smegmatis strain overexpressing MPT83 (MS_MPT83) and purifying rMPT83 protein. We found that MPT83 induced apoptosis in both human and mouse macrophages. MPT83 induced cyclooxygenase-2 (COX-2) expression at both the transcriptional and protein levels in ...
Mycobacteriophages are viruses that infect mycobacterial hosts such as Mycobacterium smegmatis and Mycobacterium tuberculosis. All mycobacteriophages characterized to date are dsDNA tailed phages, and have either siphoviral or myoviral morphotypes. However, their genetic diversity is considerable, and although sixty-two genomes have been sequenced and comparatively analyzed, these likely represent only a small portion of the diversity of the mycobacteriophage population at large. Here we report the isolation, sequencing and comparative genomic analysis of 18 new mycobacteriophages isolated from geographically distinct locations within the United States. Although no clear correlation between location and genome type can be discerned, these genomes expand our knowledge of mycobacteriophage diversity and enhance our understanding of the roles of mobile elements in viral evolution. Expansion of the number of mycobacteriophages grouped within Cluster A provides insights into the basis of immune specificity
134 23. Geier H, et al. (2008) Autoinducer-2 triggers the oxidative stress response in Mycobacterium avium, leading to biofilm formation. Appl. Environ. Microbiol. 74, 1798-1804. 24. Gopalaswamy R, et al. (2008) Mycobacterium smegmatis biofilm formation and sliding motility are affected by the serine/threonine protein kinase PknF. FEMS Microbiol. Lett. 278, 121-127. 25. Hanlon WA, et al. (1997) Pkn9, a Ser/Thr protein kinase involved in the development of Myxococcus xanthus. Mol. Microbiol. 23, 459-471. 26. Harper C, et al. (2008) Regulation of nitrogen metabolism in Mycobacterium tuberculosis: a comparison with mechanisms in Corynebacterium glutamicum and Streptomyces coelicolor. IUBMB life 60, 643-650. 27. Houben EN, et al. (2009) Differential expression of a virulence factor in pathogenic and non-pathogenic mycobacteria. Mol. Microbiol. 72, 41-52. 28. Hunter T (1995) Protein kinases and phosphatases: the yin and yang of protein phosphorylation and signaling. Cell 80, 225-236. 29. Jefferson KK ...
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Bacteria are exposed to oxidative stress as an unavoidable consequence of their aerobic lifestyle. Reactive oxygen species (ROS) are generated in the stepwise one-electron reduction of molecular oxygen during the respiration. Pathogens encounter ROS during the oxidative burst of macrophages as part of the host immune defense. Besides ROS, bacteria also have to cope with reactive chlorine, electrophilic and nitrogen species (RCS, RES, RNS). To cope with these reactive species, bacteria have evolved different defense and repair mechanisms. To maintain the reduced state of the cytoplasm, they utilize low molecular weight (LMW) thiols. LMW thiols are small thiol-containing compounds that can undergo post-translational thiolmodifications with protein thiols, termed as S-thiolations. S-thiolations function as major redox regulatory and thiol-protection mechanism under oxidative stress conditions. In eukaryotes and Gram-negative bacteria, the tripeptide glutathione (GSH) functions as major LMW thiol, which is
We found evidence that the chromosomal parA and parB genes of M. bovis BCG and M. smegmatis are expressed from multiple promoters. To identify the promoter sequences that regulate the expression of the par genes, we mapped the transcription start sites of the par-mRNAs by primer extension and confirmed the activity of the identified promoters by transcriptional fusions to a fluorescent reporter. We also demonstrated that in M. bovis BCG the parA and parB genes are differentially expressed during the exponential and stationary growth phases.. In all microorganisms studied thus far, plasmid and chromosome-encoded partitioning genes are arranged in an operon. Transcription of the par genes is driven by one (in F and R1 plasmids, P1 prophage and C. crescentus) or two (in S. coelicolor) promoters located upstream of the gene encoding the ATPase (parA or sopA) [5, 7, 13, 15, 32]. The jag, gidB, parA and parB genes of M. bovis BCG and M. smegmatis shared orientation and close spacing, suggesting that ...
Nearly 1.7 billion people are infected with Mycobacterium tuberculosis. Its ability to survive intracellularly is thought to be central to its success as a pathogen, but how it does this is poorly understood. Using a Drosophila model of infection, we identify three host cell activities, Rab7, CG8743, and the ESCRT machinery, that modulate the mycobacterial phagosome. In the absence of these factors the cell no longer restricts growth of the non-pathogen Mycobacterium smegmatis. Hence, we identify factors that represent unique vulnerabilities of the host cell, because manipulation of any one of them alone is sufficient to allow a nonpathogenic mycobacterial species to proliferate. Furthermore, we demonstrate that, in mammalian cells, the ESCRT machinery plays a conserved role in restricting bacterial growth. ...
The genus Mycobacterium comprises clinically important pathogens such as M. tuberculosis, which has reemerged as a major cause of morbidity and mortality world-wide especially with the emergence of multidrug-resistant strains. The use of fast-growing species such as Mycobacterium smegmatis has allow …
Although DNA is surprisingly fluid, there are enzymes that mediate recombination--by initiating DNA binding, strand invasion, and stabilizing ssDNA intermediates. Also, of important note, is that organisms have varying degrees of recombination levels. A classical example occurs within the Mycobacteria. Mycobacterium smegmatis has relatively low levels of illegitimate recombination (IR), while M. tuberculosis is notorious for high levels of IR compared to homologous recombination. This raises a question that can be phrased in a few ways. What enzymes are responsible for IR? or perhaps, What enzymes for homologous recombination are lacking ...
SWISS-MODEL Repository entry for A0R5E1 (CSPA_MYCS2), Probable cold shock protein A. Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacteriumsmegmatis)
Isolation and Characterization of 16 Novel Bacteriophages Infecting Mycobacterium smegmatis; Complete Genome Sequences of Three Isolates, Doom, Fascinus, and Nemo.. 110th General Meeting of the American Society for Microbiology; 05/23 - 05/27/2010 (Mark, Forsyth, Biology). Kaeser, Gwen ...
Irradiation of cytoplasm membranes of Mycobacterium smegmatis with ultraviolet light (360 nm) for 10 minutes resulted in about ... "Respiratory chains of Mycobacterium smegmatis". Indian Journal of Biochemistry & Biophysics. 12 (3): 255-259. ISSN 0301-1208. ... "Variations in the pathways of malate oxidation and phosphorylation in different species of Mycobacteria". Biochimica et ...
While originally isolated from the bacterial species Mycobacterium smegmatis and Mycobacterium tuberculosis, the causative ... A bacteriophage found to infect Mycobacterium smegmatis in 1947 was the first documented example of a mycobacteriophage. It was ... Thousands of mycobacteriophage have been isolated using a single host strain, Mycobacterium smegmatis mc2155, over 1400 of ... Gardner, Grace M.; Weiser, Russell S. (1947). "A Bacteriophage for Mycobacterium smegmatis". Proceedings of the Society for ...
214-. ISBN 978-3-540-36488-7. Hugh J O'Neill, Leon D. Gershbein (1976). "Fatty Acids of Mycobacterium Smegmatis Lipids" (PDF). ... "Purification and properties of aspartate transcarbamylase from Mycobacterium smegmatis". Biochim Biophys Acta. 953 (1): 106-113 ... 34 (1): 1-4. doi:10.1016/0378-4274(86)90138-4. PMID 3097877.CS1 maint: multiple names: authors list (link) Mycobacterium ... of Delhi people India portal Biology portal His research findings have been used by others in their researches on mycobacterium ...
Mycobacterium smegmatisMycobacterium abscessus • Neisseria species • Pseudomonas aeruginosa • Pseudomonas pyocyanea • ...
Giles is a bacteriophage that infects Mycobacterium smegmatis bacteria. The genome of this phage is very different from that of ...
Yabusaki KK; Ballou CE (1981). Long-chain fatty acyl-CoA thioesterases from Mycobacterium smegmatis. Methods in Enzymology. 71 ...
Yokoyama K, Ballou CE (December 1989). "Synthesis of alpha 1----6-mannooligosaccharides in Mycobacterium smegmatis. Function of ... This enzyme is involved in the formation of mannooligosaccharides in the membrane of Mycobacterium smegmatis. ...
"Metabolic engineering of cofactor F420 production in Mycobacterium smegmatis". PLOS One. 5 (12): e15803. doi:10.1371/journal. ...
Sengupta S, Nagaraja V (February 2008). "Inhibition of DNA gyrase activity by Mycobacterium smegmatis MurI". FEMS Microbiol. ... Sengupta S, Shah M, Nagaraja V (2006). "Glutamate racemase from Mycobacterium tuberculosis inhibits DNA gyrase by affecting its ... Sengupta S, Ghosh S, Nagaraja V (September 2008). "Moonlighting function of glutamate racemase from Mycobacterium tuberculosis ...
"Polyprenyl phosphate biosynthesis in Mycobacterium tuberculosis and Mycobacterium smegmatis". Journal of Bacteriology. 182 (20 ... Kaur D, Brennan PJ, Crick DC (November 2004). "Decaprenyl diphosphate synthesis in Mycobacterium tuberculosis". Journal of ...
Glucose Metabolism by Mycobacterium Smegmatis: Evidence for the Pentose Cycle. American Review of Respiratory Diseases. Vol. 86 ... Koch-Weser D. Book Review of Rifampin in the Treatment of Drug-Resistant Mycobacterium tuberculosis Infections" by Vall-Spinosa ...
She uses the model organisms Mycobacterium smegmatis and Escherechia coli. She was part of the team of scientists who patented ...
The trehalase enzyme of Mycobacterium smegmatis is a membrane bound protein. Periplasmic trehalase of Escherichia coli K12 is ...
FADH2 This enzyme from the bacterium Mycobacterium smegmatis participates in epimerization of trans,octacis-decaprenylphospho- ... "Analogous mechanisms of resistance to benzothiazinones and dinitrobenzamides in Mycobacterium smegmatis". PLOS ONE. 6 (11): ...
... and properties of D-ribose isomerase from Mycobacterium smegmatis". J. Biol. Chem. 250 (20): 8085-7. PMID 240851. Biology ...
"Structural characterization of peptidyl-tRNA hydrolase from Mycobacterium smegmatis by NMR spectroscopy". Biochimica et ...
"Purification and partial characterization of a penicillin-binding protein from Mycobacterium smegmatis". Journal of ...
Gilleron M, Himoudi N, Adam O, Constant P, Venisse A, Rivière M, Puzo G (January 1997). "Mycobacterium smegmatis ... purified from Mycobacterium chelonae and Mycobacterium kansasii induce TNF-alpha and IL-8 secretion by a CD14-toll-like ... These types of LAMs are most commonly found in more pathogenic Mycobacterium species such as M. tuberculosis, M. leprae, and M ... The inactivation of macrophages allows for the dissemination of mycobacteria to other parts of the body. The destruction of ...
"Role of the methylcitrate cycle in propionate metabolism and detoxification in Mycobacterium smegmatis". Microbiology. 153 (Pt ... In fact, in some bacteria such as Mycobacterium tuberculosis, isocitrate lyase actually plays the role of methylisocitrate ... "Dual role of isocitrate lyase 1 in the glyoxylate and methylcitrate cycles in Mycobacterium tuberculosis". Molecular ... "Acetyl-CoA-Mediated Activation of Mycobacterium tuberculosis Isocitrate Lyase 2". Nature Communications. 10 (1): 4639. Bibcode: ...
"Conjugative transfer of a shuttle plasmid from Escherichia coli to Mycobacterium smegmatis [corrected]". FEMS Microbiol. Lett. ... Her publications from these years focused on mycobacterium and mycoplasmas, specifically genetic and molecular characterization ... "Restriction endonuclease mapping and cloning of Mycobacterium fortuitum var. fortuitum plasmid pAL5000". Ann Inst Pasteur ...
"Purification and characterization of a novel mycolic acid exchange enzyme from Mycobacterium smegmatis". J. Biol. Chem. 262 (28 ...
... from Mycobacterium smegmatis". Scientific Reports. 9 (1): 18019. Bibcode:2019NatSR...918019N. doi:10.1038/s41598-019-53736-8. ... In Mycobacteria, the ClpXP protease is composed of the ATPase component (ClpX) and two ClpP proteins (ClpP1 and ClpP2). ... Schmitz KR, Carney DW, Sello JK, Sauer RT (October 2014). "Crystal structure of Mycobacterium tuberculosis ClpP1P2 suggests a ... Leodolter J, Warweg J, Weber-Ban E (2015-05-01). Zeth K (ed.). "The Mycobacterium tuberculosis ClpP1P2 Protease Interacts ...
"Role of the methylcitrate cycle in propionate metabolism and detoxification in Mycobacterium smegmatis". Microbiology. 153 (Pt ... In Mycobacterium tuberculosis, it has been suggested that the metabolism of propionyl-CoA is involved in cell wall biogenesis. ... It is also attributed as a resulting pathway due to the catabolism of fatty acids in mycobacteria. In order to proceed, the ... Muñoz-Elías EJ, Upton AM, Cherian J, McKinney JD (June 2006). "Role of the methylcitrate cycle in Mycobacterium tuberculosis ...
... from Mycobacterium smegmatis". Journal of Structural Biology. 196 (3): 448-454. doi:10.1016/j.jsb.2016.09.012. PMID 27659385. ... His researches are known to have assisted in a wider understanding of the biology of mycobacterium, more specifically the ... Science and Technology and is known for his studies on protein synthesis and DNA repair in Escherichia coli and Mycobacterium ... researches are primarily focused in the area of protein synthesis and DNA repair in Escherichia coli and Mycobacterium ...
Kushwaha, Ambuj K.; Grove, Anne (2013-02-01). "C-terminal low-complexity sequence repeats of Mycobacterium smegmatis Ku ...
... of Mycobacterium smegmatis". FEBS Lett. 580 (17): 4031-41. doi:10.1016/j.febslet.2006.06.037. PMID 16814781. Yeh DC, ... Pathania R, Navani NK, Rajamohan G, Dikshit KL (May 2002). "Mycobacterium tuberculosis hemoglobin HbO associates with membranes ...
... a key gene in the mycothiol biosynthesis pathway in Mycobacterium smegmatis". Microbiology. 149 (Pt 5): 1341-9. doi:10.1099/mic ... from Mycobacterium tuberculosis reveals a zinc hydrolase with a lactate dehydrogenase fold". The Journal of Biological ...
Sources of this enzyme includes Micrococcus luteus, Phaseolus aureus, Mycobacterium smegmatis and cotton fibers. Lahav M, Chiu ...
Tremblay LW, Fan F, Vetting MW, Blanchard JS (December 2008). "The 1.6 A crystal structure of Mycobacterium smegmatis MshC: the ... "Steady-state and pre-steady-state kinetic analysis of Mycobacterium smegmatis cysteine ligase (MshC)". Biochemistry. 46 (40): ... glutathione-S-transferase and maltose binding protein fusion proteins in Mycobacterium smegmatis" (PDF). Protein Expression and ...
Protein nanopore sequencing utilizes membrane protein complexes such as α-hemolysin, MspA (Mycobacterium smegmatis Porin A) or ...
M. smegmatis group. *M. agri/M. thermoresistibile. *M. duvalii/M. flavescens. *M. monacense ... Mycobacterium bovis. Karlson & Lessel 1970,[1] ATCC 19210. Mycobacterium bovis (M. bovis) is a slow-growing (16- to 20-hour ... Mycobacterium bovis can be transmitted from human to human; there was an outbreak in Birmingham, England in 2004,[33] and from ... Karlson, A. G.; Lessel, E. F. (1970). "Mycobacterium bovis nom. nov". International Journal of Systematic Bacteriology. 20 (3 ...
Mycobacterium smegmatis mutant strains that are deficient in the ability to repair double-strand breaks by the non-homologous ... "NHEJ protects mycobacteria in stationary phase against the harmful effects of desiccation" (PDF). DNA Repair (Amst.). 6 (9): ...
Chemistry of the Lyxose-Containing Mycobacteriophage Receptors of Mycobacterium phlei/Mycobacterium smegmatis, Biochemistry, 35 ...
... but also occurs in other bacteria such as Mycobacterium smegmatis. Conjugation requires stable and extended contact between a ... "Distributive conjugal transfer in mycobacteria generates progeny with meiotic-like genome-wide mosaicism, allowing mapping of ...
... not only in this species but also in Mycobacterium smegmatis). The bacterial filament ultrastructure is consistent with ... A similar mechanism may occur in Mycobacterium tuberculosis,which also elongates after being phagocytized. Ssd encoded by ... England, Kathleen; Rebecca Crew; Richard A Slayden (2011). "Mycobacterium tuberculosis septum site determining protein, Ssd ...
They show weak antibiotic action against Staphylococcus aureus, Mycobacterium smegmatis, and Candida albicans.[5] ...
M. smegmatis(英语:Mycobacterium smegmatis) *M. goodii(英语:Mycobacterium goodii) ... M. canetti(英语:Mycobacterium canetti). M. caprae(英语:Mycobacterium caprae). M. microti(英语:Mycobacterium microti). M. pinnipedii(英 ... M. indicus pranii(英语:Mycobacterium indicus pranii). 戈登分枝杆菌 Mycobacterium gordonae clade编辑. *M. asiaticum(英语:Mycobacterium ... M. kansasii(英语:Mycobacterium kansasii). Mycobacterium nonchromogenicum/terrae clade编辑. *M. hiberniae(英语:Mycobacterium
M. smegmatis group. *M. agri/M. thermoresistibile. *M. duvalii/M. flavescens. *M. monacense ... Mycobacterium avium complex is a group of mycobacteria comprising Mycobacterium intracellulare, Mycobacterium avium, and ... Pathogenic mycobacteria. Advances in Tuberculosis Research, 14, 235-287.. *^ "Mycobacterium Avium Complex. MAI; MAC Information ... These bacteria cause disease in humans called Mycobacterium avium-intracellulare infection or Mycobacterium avium complex ...
... smegmatis, in to kar za tisočkrat v primerjavi s celičnimi kulturami brez dodanih mineralnih produktov.«[12] ... povzroča pa je bakterija Mycobacterium ulcerans. ...
M. smegmatis group. *M. agri/M. thermoresistibile. *M. duvalii/M. flavescens. *M. monacense ... Mycobacterium lepromatosis is a bacterium that, along with Mycobacterium leprae, causes leprosy (Hansen's disease). It was only ... "Comparative Sequence Analysis of Mycobacterium leprae and the New Leprosy-Causing Mycobacterium lepromatosis". Journal of ... "A New Mycobacterium Species Causing Diffuse Lepromatous Leprosy". American Journal of Clinical Pathology. 130 (6): 856-64. doi ...
Posebno bogat prirodni izvor F420 je Mycobacterium smegmatis, u kojoj nekoliko desetina enzima koristi F420 umesto srodnog ... Selengut JD, Haft DH (2010). „Unexpected abundance of coenzyme F(420)-dependent enzymes in Mycobacterium tuberculosis and other ...
All Mycobacteria - M. tuberculosis, M. leprae, M. smegmatis and atypical Mycobacterium. *Actinomycetes (especially some aerobic ... The acid-fastness of Mycobacteria is due to the high mycolic acid content of their cell walls, which is responsible for the ... Some, such as Mycobacteria, can be stained with the Gram stain, but they do not take the crystal violet well and thus appear ... Acid-fast Mycobacteria can also be visualized by fluorescence microscopy using specific fluorescent dyes (auramine-rhodamine ...
... was previously known as Mycobacterium smegmatis group 2. Brown et al. 1999. Mycobacterium wolinskyi sp. ... and Mycobacterium goodii sp. nov., two new rapidly growing species related to Mycobacterium smegmatis and associated with human ... Mycobacterium goodii is an acid-fast bacterial species in the phylum Actinobacteria and the genus Mycobacterium. M. goodii ... M. goodii is found in many of the same settings as M. smegmatis and members of the M. fortuitum complex. It can cause post- ...
... and Mycobacterium smegmatis. On the other hand, H. utriformis has low antifungal activity against the species Candida albicans ...
2 The enzyme from the bacterium Mycobacterium smegmatis is specific for maltose. Elbein AD, Pastuszak I, Tackett AJ, Wilson T, ...
... is a cyclic di-AMP-responsive repressor in Mycobacterium smegmatis". The Journal of Biological Chemistry. 288 (5): 3085-96. doi ... Cyclic di-AMP has been linked to fatty acid synthesis regulation in Myobacterium smegmatis, the growth of S. aureus in ...
Mycobacterium smegmatis mutant strains that are deficient in the ability to repair double-strand breaks by the non-homologous ... Pitcher RS, Green AJ, Brzostek A, Korycka-Machala M, Dziadek J, Doherty AJ (2007). "NHEJ protects mycobacteria in stationary ...
... smegmatis M. goodii M. wolinskyi M. sphagni M. thermoresistibile M. vanbaalenii M. arosiense M. aubagnense M. chlorophenolicum ... Structures of Mycobacterium tuberculosis proteins MycDB: Mycobacterium database TBDB: Tuberculosis database Mycobacterium ... Mycobacteria appear phenotypically most closely related to members of Nocardia, Rhodococcus, and Corynebacterium. Mycobacteria ... Runyon's group I, II and III Also see main article about Mycobacterium tuberculosis complex Mycobacterium tuberculosis complex ...
Mycobacterium smegmatis small RNA 1 (Ms1 small RNA) is highly expressed during stationary phase of growth, Ms1 RNA directly ... There is evidence that Ms1 RNA may function similar to 6S RNA in M. smegmatis which does not have 6S RNA. Hnilicová J, Jirát ... a novel sRNA interacting with the RNA polymerase core in mycobacteria". Nucleic Acids Research. 42 (18): 11763-11776. doi: ...
Bruender NA, Bandarian V (2017). "The Creatininase Homolog MftE from Mycobacterium smegmatis Catalyzes a Peptide Cleavage ... The name "mycofactocin" is derived from three words, the genus name "Mycobacterium" (across which it is nearly universal), " ... However, its species distribution is heavily skewed towards the Actinobacteria, including Mycobacterium tuberculosis, which is ... family alone in Mycobacterium avium. The mycofactocin biosynthesis pathway is one of the most abundant of any RiPP system in ...
Mycobacterium smegmatis is 3.0 to 5.0 µm long with a bacillus shape, an acid-fast bacterial species in the phylum ... M. smegmatis is useful for the research analysis of other Mycobacteria species in laboratory experiments. Since it is a fast ... The M. smegmatis strain is now the work-horse of mycobacterial genetics. It is readily available in most laboratory media and ... This organism was later named M. smegmatis. It is considered a non-pathogenic microorganism although, in rare cases, it can ...
Mycobacterium smegmatis is an acid-fast bacterial species in the phylum Actinobacteria and the genus Mycobacterium. It is 3.0 ... M. smegmatis is useful for the research analysis of other Mycobacteria species in laboratory experiments. M. smegmatis is ... Information and photo from NCBI MicrobeWiki page on M. smegmatis Type strain of Mycobacterium smegmatis at BacDive - the ... I. Species descriptions of Mycobacterium phlei Lehmann and Neumann and Mycobacterium smegmatis (Trevisan) Lehmann and Neumann ...
L,D-transpeptidase from Mycobacterium smegmatis. Osipiuk, J., Wu, R., Endres, M., Joachimiak, A., Midwest Center for Structural ... Mycolicibacterium smegmatis MC2 155. Mutation(s): 0 Gene Names: MSMEI_3449. Find proteins for I7G323 (Mycolicibacterium ...
Mycobacterium smegmatis ATCC ® 607™ Designation: TypeStrain=False Application: Assay of bleomycin Bacteriophage host ... Nucleotide (GenBank) : X60513 M.smegmatis gltA gene for citrate synthase. Nucleotide (GenBank) : U07954 Mycobacterium smegmatis ... I. Species descriptions of Mycobacterium phlei Lehmann and Neumann and Mycobacterium smegmatis (Trevisan) Lehmann and Neumann ... Mycobacterium smegmatis (Trevisan) Lehmann and Neumann (ATCC® 607™) Type Strain: no / Biosafety Level: 1 ...
Mycobacterium smegmatis, and a TNFalpha gene-modified recombinant M. smegmatis. When wild-type M. smegmatis were delivered to ... Cytokine-modified Mycobacterium smegmatis as a novel anticancer immunotherapy.. Young SL1, Murphy M, Zhu XW, Harnden P, ... Recombinant M. smegmatis secreting TNFalpha, however, gave a 70% durable tumour-free survival. Lymphocytes from draining lymph- ... No toxicity was observed with wild-type or recombinant M. smegmatis in immunocompetent, T-deficient or NK-deficient models. We ...
Mycobacterium smegmatis ( German ) provided by wikipedia DE Wissenschaftlicher Name Mycobacterium smegmatis (Trevisan 1889) ... Mycobacterium smegmatis: Brief Summary ( German ) provided by wikipedia DE Mycobacterium smegmatis ist ein Bakterium aus der ... Mycobacterium smegmatis ist eine Art in der Gattung Mycobacterium in der Familie der Mycobacteriaceae, die zur Abteilung ... Zusammen mit Mycobacterium goodii wird es in die Mycobacterium smegmatis-Clade gestellt.[1] ...
Mycobacterium vaccae ATCC 25954. Mycobacterium sp. SWH-M1. Mycobacterium peregrinum. Mycobacterium mucogenicum. Mycobacterium ... Mycobacterium boenickei. Mycobacterium septicum. Mycobacterium sp. NAZ190054. Mycobacterium bacteremicum. Mycobacterium ... Mycobacterium sp. UNC267MFSha1.1M11. Mycobacterium bacteremicum. Mycobacterium sp. Soil538. Mycobacterium sp. NAZ190054. And ... Mycobacterium gilvum (strain DSM 45189 / LMG 24558 / Spyr1). Mycobacterium vanbaalenii (strain DSM 7251 / PYR-1). Mycobacterium ...
New tRNA contacts facilitate ligand binding in a Mycobacterium smegmatis T box riboswitch. Anna V. Sherwood, Jane K. Frandsen, ... In this study, the role of the conserved elements of the Mycobacterium smegmatis ileS T box riboswitch in tRNAIle binding was ... In this study, structural elements of a Mycobacterium smegmatis ileS T box riboswitch variant with an Ultrashort (US) Stem I ... New tRNA contacts facilitate ligand binding in a Mycobacterium smegmatis T box riboswitch ...
Crystal structure of deoxyribose phosphate aldolase from mycobacterium smegmatis. *DOI: 10.2210/pdb3NDO/pdb ... Mycolicibacterium smegmatis. Mutation(s): 0 Gene Names: deoC, MSMEG_0922. EC: 4.1.2.4. ... Find proteins for A0QQY7 (Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)) ... High-resolution three-dimensional structures of essential Mycobacterium tuberculosis (Mtb) proteins provide templates for TB ...
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155). Mycobacterium smegmatis. Mycobacterium smegmatis (strain MKD8). 675. ... Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155). Mycobacterium smegmatis. Mycobacterium smegmatis (strain MKD8). ... Mycobacterium sp. 1274761.0. Mycobacterium sp. 1482268.1. Mycobacterium sp.. Mycobacterium flavescens. Mycobacterium sp. GA- ... Mycobacterium smegmatis. ,p>This subsection of the Names and taxonomy section shows the unique identifier assigned by the , ...
Using Mycobacterium smegmatis as a fast growing model for Mycobacterium tuberculosis, we demonstrate that dUTPase knock-out ... In addition, here we demonstrated that Mycobacterium tuberculosis dUTPase is fully functional in Mycobacterium smegmatis as it ... We also show that deletion of the mycobacteria-specific loop has no major effect on dUTPase enzymatic properties in vitro and ... All mycobacterial genomes encode dUTPase with a mycobacteria-specific surface loop absent in the human dUTPase. ...
smegmatis were isolated in a biological active state by diff ... spheroplasts induced by lysozyme or glycine from Mycobacterium ... Isolation problems and structural organization of membrane units in Mycobacterium sp. smegmatis *P. Mišoň1. , ... Membrane units from lysed spheroplasts induced by lysozyme or glycine from Mycobacterium spec. smegmatis were isolated in a ... Mišoň, P., Trnka, L., Mohelská, H. et al. Isolation problems and structural organization of membrane units in Mycobacterium sp ...
Nucleotide excision repair - Mycobacterium smegmatis MC2 155 [ Pathway menu , Organism menu , Pathway entry , Download KGML , ...
By using the rapid-growing Mycobacterium smegmatis as a model genetic system, a gene was selected that confers low-level FQ ... Efflux pump of the proton antiporter family confers low-level fluoroquinolone resistance in Mycobacterium smegmatis. H E Takiff ... Efflux pump of the proton antiporter family confers low-level fluoroquinolone resistance in Mycobacterium smegmatis ... Efflux pump of the proton antiporter family confers low-level fluoroquinolone resistance in Mycobacterium smegmatis ...
IS1549 from Mycobacterium smegmatis Forms Long Direct Repeats upon Insertion. Bonnie B. Plikaytis, Jack T. Crawford, Thomas M. ... IS1549 from Mycobacterium smegmatis Forms Long Direct Repeats upon Insertion. Bonnie B. Plikaytis, Jack T. Crawford, Thomas M. ... IS1549 from Mycobacterium smegmatis Forms Long Direct Repeats upon Insertion. Bonnie B. Plikaytis, Jack T. Crawford, Thomas M. ... IS1549 from Mycobacterium smegmatis Forms Long Direct Repeats upon Insertion Message Subject (Your Name) has forwarded a page ...
The specialized secretory apparatus ESX-1 is essential for DNA transfer in Mycobacterium smegmatis.. Coros A1, Callahan B, ... Conjugal DNA transfer in Mycobacterium smegmatis occurs by a mechanism distinct from plasmid-mediated DNA transfer. Previously ... The Specialized secretory apparatus ESX-1 is essential for DNA transfer in Mycobacterium smegmatis ... The Specialized secretory apparatus ESX-1 is essential for DNA transfer in Mycobacterium smegmatis ...
Paralogous cAMP receptor proteins in Mycobacterium smegmatis show biochemical and functional divergence.. [Ritu Sharma, Anisha ... Here, we identify two paralogous CRPs in the genome of Mycobacterium smegmatis that have 78% identical sequences and ...
... proton antiporter isolated from Mycobacterium smegmatis; amino acid sequence in first source; GenBank U40487 ... Mycobacterium smegmatis LfrA protein. Subscribe to New Research on Mycobacterium smegmatis LfrA protein ... proton antiporter isolated from Mycobacterium smegmatis; amino acid sequence in first source; GenBank U40487 ...
Mycobacterium smegmatis serves as a model for studying mycobacterial pathogens including M. tuberculosis. M. smegmatis encodes ... M. smegmatis serves as a model for studying mycobacterial pathogens including M. tuberculosis. M. smegmatis encodes two ... Mycobacterium tuberculosis, the causative agent of tuberculosis, requires its phosphate sensing signal transduction system for ... Our results suggest that M. smegmatis can serve as a tractable model for further characterization of the molecular mechanism of ...
... smegmatis and Mycobacterium bovisBCG and the integrative cosmid pYUB412 (AprHygr) (4). The M. smegmatis library was a gift from ... NADH Dehydrogenase Defects Confer Isoniazid Resistance and Conditional Lethality in Mycobacterium smegmatis Lynn Miesel, Torin ... 1990) Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis. Mol. Microbiol. 4: ... 1994) Effects of peroxides on susceptibilities of Escherichia coli and Mycobacterium smegmatis to isoniazid. Antimicrob. Agents ...
MSMEG_2964 Recombinant Protein (Mycobacterium smegmatis) (OPCA13159) Catalog #: OPCA13159 Predicted Species: Mycobacterium ... MSMEG_4483 Recombinant Protein (Mycobacterium smegmatis) (OPCA186814) Catalog #: OPCA186814 Predicted Species: Mycobacterium ... MSMEG_6896 Recombinant Protein (Mycobacterium smegmatis) (OPCA321113) Catalog #: OPCA321113 Predicted Species: Mycobacterium ... GLTA Recombinant Protein (Mycobacterium smegmatis) (OPCA16873) Catalog #: OPCA16873 Predicted Species: Mycobacterium smegmatis ...
MspA is the major porin of Mycobacterium smegmatis. In this study we showed that three paralogues of MspA, namely MspB, MspC ... The growth rate of Mycobacterium smegmatis depends on sufficient porin-mediated influx of nutrients.. [Joachim Stephan, ... Mycobacteria have a unique outer membrane (OM) that is thicker than any other known biological membrane. Nutrients cross this ... It is concluded that mspB and mspD provide backup porins for M. smegmatis. This also indicated that a minimal porin-mediated OM ...
Mycobacterium bovis BCG and Mycobacterium smegmatis (M. smegmatis), by damaging the bacterial cell membrane. Here, we have ... Mycobacterium bovis BCG and Mycobacterium smegmatis (M.smegmatis), by damaging the bacterial cell membrane. Here, we have ... Our results demonstrated that exogenous PAF C-16 inhibited the growth of M.smegmatis inside phagocytic cells of monocytic cell ... Our results demonstrated that exogenous PAF C-16 inhibited the growth of M. smegmatis inside phagocytic cells of monocytic cell ...
The PNAs targeting the pncA, polA and rpoC genes were found to exhibit strong growth inhibition against M. smegmatis but only ... No growth inhibition of M. smegmatis was seen in pure culture when treated with PNAs targeting gyrA and a mismatch PNA ... Results: In Middlebrook broth, the strong growth inhibitory effect against M. smegmatis was observed by PNAs targeting the inhA ... the efficacy of several antisense PNAs in inhibiting extracellular and intracellular growth of Mycobacterium smegmatis. Methods ...
Mycobacterium smegmatis possesses two such transporters, the widely distributed PstSCAB system an … ... Mycobacterium smegmatis possesses two such transporters, the widely distributed PstSCAB system and PhnDCE, a transporter that ... Crystal Structure of PhnF, a GntR-family Transcriptional Regulator of Phosphate Transport in Mycobacterium Smegmatis J ... In contrast, pathogenic mycobacteria were not found to encode Phn-like systems but instead were found to possess multiple ...
Mycobacterium smegmatis str. MC2 155). Find diseases associated with this biological target and compounds tested against it in ...
Biochemical characterization of the 49 kDa penicillin-binding protein of Mycobacterium smegmatis Tapan MUKHERJEE Tapan ... The 49 kDa penicillin-binding protein (PBP) of Mycobacterium smegmatis catalyses the hydrolysis of the peptide or S-ester bond ... Biochemical characterization of the 49 kDa penicillin-binding protein of Mycobacterium smegmatis. Biochem J 15 November 1996; ...
To evaluate the intracellular accumulation of norfloxacin in mycobacteria, two methods were used with Mycobacterium smegmatis. ... Intracellular accumulation of norfloxacin in Mycobacterium smegmatis. Message Subject (Your Name) has forwarded a page to you ... Intracellular accumulation of norfloxacin in Mycobacterium smegmatis.. S Corti, J Chevalier, A Cremieux ... Thus, the same mechanisms of quinolone accumulation that occur in eubacteria seem to occur in mycobacteria, at least in M. ...
Ribosylative inactivation of rifampin by Mycobacterium smegmatis is a principal contributor to its low susceptibility to this ... Intermediates of Rifampicin-ribosylation by Mycobacterium smegmatis DSM43756 * * MORISAKI Naoko ... and characterization of a new intermediate in the ribosylative inactivation pathway of rifampin by Mycobacterium smegmatis IMAI ...
Ergothioneine Is a Secreted Antioxidant in Mycobacterium smegmatis. Carine Sao Emani, Monique J. Williams, Ian J. Wiid, ... Ergothioneine Is a Secreted Antioxidant in Mycobacterium smegmatis. Carine Sao Emani, Monique J. Williams, Ian J. Wiid, ... Ergothioneine Is a Secreted Antioxidant in Mycobacterium smegmatis. Carine Sao Emani, Monique J. Williams, Ian J. Wiid, ... Mycothiol import by Mycobacterium smegmatis and function as a resource for metabolic precursors and energy production. J. ...
  • Mycobacterium smegmatis: an absurd model for tuberculosis? (eol.org)
  • High-resolution three-dimensional structures of essential Mycobacterium tuberculosis (Mtb) proteins provide templates for TB drug design, but are available for only a small fraction of the Mtb proteome. (rcsb.org)
  • Is the Subject Area "Mycobacterium tuberculosis" applicable to this article? (plos.org)
  • Due to the resurgence of tuberculosis and the emergence of multidrug-resistant strains, fluoroquinolones (FQ) are being used in selected tuberculosis patients, but FQ-resistant strains of Mycobacterium tuberculosis have rapidly begun to appear. (pnas.org)
  • DNA transfer therefore provides a simple molecular genetic assay to characterize ESX-1, which, in Mycobacterium tuberculosis, is necessary for full virulence. (nih.gov)
  • Moreover, our observation that ESX-1 has such diametrically opposed effects on transfer in the donor and recipient, forces us to consider how proteins secreted by the ESX-1 apparatus can function so as to modulate two seemingly disparate processes, M. smegmatis DNA transfer and M. tuberculosis virulence. (nih.gov)
  • A genetic map comparing the esx-1 loci of M. smegmatis and M. tuberculosis . (nih.gov)
  • Mycobacterium tuberculosis , the causative agent of tuberculosis, requires its phosphate sensing signal transduction system for virulence and antibiotic tolerance, but the molecular mechanisms of phosphate sensing remain poorly characterized. (frontiersin.org)
  • Isoniazid (INH) is a highly effective drug used in the treatment and prophylaxis of Mycobacterium tuberculosis infections. (asm.org)
  • Mycobacterium tuberculosis is exquisitely sensitive to the drug isoniazid (INH), and this sensitivity has made INH the core of tuberculosis chemotherapy and prophylaxis since the early 1950s ( 1 , 37 ). (asm.org)
  • In our effort to develop better tuberculosis drugs, we are trying to understand how INH kills sensitive mycobacteria and how mycobacteria become resistant. (asm.org)
  • The InhA enzyme catalyzes an essential step in fatty acid elongation and mycolic acid synthesis and is a target for INH action in M. tuberculosis and the soil saprophyte Mycobacterium smegmatis ( 3 , 36 ). (asm.org)
  • Mycobacterium tuberculosis ( M.tb ) infection results in approximately 1.3 million human deaths each year. (frontiersin.org)
  • Mycobacterium tuberculosis ( M.tb ) belongs to the acid-fast group of bacteria and causes an infectious disease in humans, known as Tuberculosis (TB), which mostly affects the respiratory system. (frontiersin.org)
  • Mycobacterium smegmatis is an avirulent bacterial genus present in the soil and smegma that shows many features similar to Mycobacterium tuberculosis ( Mtb ), responsible for causing tuberculosis in humans [1]. (scirp.org)
  • The MSH-deficient Δ mshA mutant of Mycobacterium tuberculosis requires catalase during in vitro growth, implicating MSH in detoxifying reactive oxygen species ( 10 ). (asm.org)
  • Because of this, Mycobacterium tuberculosis (Mtb) is more rapidly killed using a combination of drugs capable of PBP- and LDT- inhibition. (elifesciences.org)
  • However, in a group of bacteria called mycobacteria, which cause tuberculosis and other diseases, the units are added at the tips. (elifesciences.org)
  • But, drugs that treat tuberculosis do not attack this layer, partly because we know very little about the cell walls of mycobacteria. (elifesciences.org)
  • In fact, there are important differences between model bacteria and Actinobacteria like mycobacteria, a genus of rod-shaped bacteria that includes the major human pathogen Mycobacterium tuberculosis (Mtb). (elifesciences.org)
  • It contributes to the virulence of Mycobacterium tuberculosis. (jic.ac.uk)
  • The recent resurgence of drug resistant strains of Mycobacterium tuberculosis, the causal agent of tuberculosis (TB) has strengthened the need for new anti-TB drugs. (bl.uk)
  • Evaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studies. (sld.cu)
  • Preliminary studies of humoral immunogenicity and cross-reactivity with M. tuberculosis in mice using two M. smegmatis- derived experimental vaccines were carried out, demonstrating the immunogenicity of M. smegmatis proteoliposomes and the recognition of M. tuberculosis proteins by the sera of animals immunized with this vaccine candidate. (sld.cu)
  • The conjunction of in silico and in vivo studies suggested the potential for future evaluation of M. smegmatis as vaccine candidate against tuberculosis using different strategies. (sld.cu)
  • En este estudio se revisaron múltiples bases de datos publicadas, relacionadas con experimentos de expresión de genes de M. tuberculosis in vivo en diferentes estadios de la infeccción en humanos y animales. (sld.cu)
  • Se llevaron a cabo estudios de inmunogenicidad humoral y reactividad cruzada con M. tuberculosis en ratones inmunizados con dos vacunas experimentales obtenidas a partir de M. smegmatis , demostrándose la immunogenicidad de los proteoliposomas y el reconocimiento de proteínas de M. tuberculosis por el suero de ratones vacunados con este candidato vacunal. (sld.cu)
  • Los resultados obtenidos con los estudios in sílico e in vivo sugieren la potencialidad para evaluación futura de candidatos vacunales obtenidos a partir de M. smegmatis para la prevención de la tuberculosis. (sld.cu)
  • Mycobacterium tuberculosis , the main agent of tuberculosis (TB) in humans caused 9.2 million new cases and 1.7 million deaths in 2006. (sld.cu)
  • With the complete genome sequence of M. tuberculosis and the advances in the sequencing of BCG and other mycobacteria, an enormous flow of valuable information has been made available for new vaccine strategies (2, 3). (sld.cu)
  • We investigated the use of a tetracycline-inducible expression system in Mycobacterium tuberculosis and Mycobacterium smegmatis . (asm.org)
  • The chromosomal regions surrounding the origins of DNA replication from M. smegmatis, M. tuberculosis, and M. leprae have been sequenced, and show very few differences. (epfl.ch)
  • By means of subcloning experiments, the putative chromosomal origin of replication of M. smegmatis, containing the dnaA-dnaN region, was shown to promote autonomous replication in M. smegmatis, unlike the corresponding regions from M. tuberculosis or M. leprae. (epfl.ch)
  • The truncated Rv2820c of Mycobacterium tuberculosis Beijing family augments intracellular. (deepdyve.com)
  • Bao, Lang 2018-04-01 00:00:00 Genetic variations among genes of Mycobacterium tuberculosis may be associated with antigenic variation and immune evasion, which complicates the pathogenesis of M. tuberculosis. (deepdyve.com)
  • It has been reported that Rv2820c-Bj correlated with enhanced intracellular survival of M. smegmatis in macrophages when compared to its full-length counterpart Rv2820c in M. tuberculosis, however, the respective contribution of the truncation and the new 3′-end of Rv2820c-Bj to this enhancement was unclear. (deepdyve.com)
  • Recombinant Mycobacterium smegmatis expressing an ESAT6-CFP10 fusion protein induces anti-mycobacterial immune responses and protects against Mycobacterium tuberculosis challenge in mice. (semanticscholar.org)
  • Recombinant Bacillus subtilis spores for the delivery of Mycobacterium tuberculosis Ag85B-CFP10 secretory antigens. (semanticscholar.org)
  • The mc2-CMX vaccine induces an enhanced immune response against Mycobacterium tuberculosis compared to Bacillus Calmette-Guérin but with similar lung inflammatory effects. (semanticscholar.org)
  • Prime-boost vaccination with Bacillus Calmette Guerin and a recombinant adenovirus co-expressing CFP10, ESAT6, Ag85A and Ag85B of Mycobacterium tuberculosis induces robust antigen-specific immune responses in mice. (semanticscholar.org)
  • Mycobacterium tuberculosis (Mtb) and Mycobacterium smegmatis (Msmeg) can grow on cholesterol as the sole carbon source. (escholarship.org)
  • The serine/threonine kinase PknB of Mycobacterium tuberculosis phosphorylates PBPA, a penicillin-binding protein required for cell division. (microbiologyresearch.org)
  • Contribution of the Mycobacterium tuberculosis MmpL protein family to virulence and drug resistance. (microbiologyresearch.org)
  • Inhibition of mycolic acid transport across the Mycobacterium tuberculosis plasma membrane. (microbiologyresearch.org)
  • Therapeutic potential of the Mycobacterium tuberculosis mycolic acid transporter, MmpL3. (microbiologyresearch.org)
  • Mycobacterium smegmatis is a saprophytic bacterium frequently used as a genetic surrogate to study pathogenic Mycobacterium tuberculosis . (biomedcentral.com)
  • In this study, transcriptomic analysis (RNA-seq) of an M. smegmatis Δ prrAB mutant was used to define the PrrAB regulon and provide insights into the essential nature of PrrAB in M. tuberculosis . (biomedcentral.com)
  • PrrAB induced transcription of dosR ( devR ), a response regulator gene that promotes latent infection in M. tuberculosis and 21 of the 25 M. smegmatis DosRS regulon homologues. (biomedcentral.com)
  • Mycobacterium tuberculosis , the causative agent of tuberculosis, is an ancient disease of mankind and the leading cause of death from an infectious agent [ 12 ]. (biomedcentral.com)
  • The role of several energy-driven transport systems and the effects of anti- tubercular compounds on cellular and ion homeostasis in M. smegmatis and Mycobacterium tuberculosis were also studied. (otago.ac.nz)
  • MSH is produced by Mycobacterium tuberculosis, members of the Actinomycetes family, to maintain an intracellular reducing environment and protect against oxidative and antibiotic induced stress. (elsevier.com)
  • However, porins that could mediate the diffusion of phosphates across the OM of M. bovis BCG and Mycobacterium tuberculosis are unknown. (uab.edu)
  • 3. Rao V , Gao F , Chen B , Jacobs WR , Glickman MS . Trans-cyclopropanation of mycolic acids on trehalose dimycolate suppresses Mycobacterium tuberculosis -induced inflammation and virulence. (microbiologyresearch.org)
  • The anti-tuberculosis drug, ethambutol (Emb), was previously shown to inhibit the synthesis of arabinans of both the cell wall arabinogalactan (AG) and lipoarabinomannan (LAM) of Mycobacterium tuberculosis and other mycobacteria. (semanticscholar.org)
  • In this report the characterization of complexes of Ugi with UDGs from Mycobacterium tuberculosis, a pathogenic bacterium, and Mycobacterium smegmatis, a widely used model organism for the former, is described. (iisc.ernet.in)
  • However, the M. tuberculosis (Mtu) UDG-Ugi complex was seen when MtuUDG was incubated with M. smegmatis (Msm) UDG-Ugi or EcoUDG(L191G)-Ugi complexes. (iisc.ernet.in)
  • The ESAT-6 (early secreted antigenic target, 6 kDa) family collects small mycobacterial proteins secreted by Mycobacterium tuberculosis , particularly in the early phase of growth. (beds.ac.uk)
  • In this research, we performed EMSA experiments and transcriptional analysis of ESAT-6 cluster 3 in Mycobacterium smegmatis ( msmeg0615 - msmeg0625 ) and M. tuberculosis . (beds.ac.uk)
  • In contrast to what we had observed in M. tuberculosis , we found that in M. smegmatis ESAT-6 cluster 3 responds only to iron and not to zinc. (beds.ac.uk)
  • In contrast with what has been reported for M. tuberculosis , our results suggest that in M. smegmatis only IdeR-dependent regulation is retained, while zinc has no effect on gene expression. (beds.ac.uk)
  • Differences in expression between cluster 3 genes can be explained by the presence of internal promoters, both in M. smegmatis and M. tuberculosis . (beds.ac.uk)
  • The gene encoding methionine adenosyltransferase has been cloned from Mycobacterium tuberculosis and the model organism M. smegmatis . (beds.ac.uk)
  • While the M. smegmatis enzyme could be functionally expressed, the M. tuberculosis homologue was insoluble and inactive under a large variety of expression conditions. (beds.ac.uk)
  • Mycobacterium tuberculosis is difficult to kill for a number of reasons. (beds.ac.uk)
  • In the examination of biochemical pathways in Mycobacterium tuberculosis , it would be ideal to identify processes where an enzyme plays a role in both active and chronic phase survival. (beds.ac.uk)
  • Interaction of Erp Protein of Mycobacterium tuberculosis with Rv2212 Enhances Intracellular Survival of Mycobacterium smegmatis. (osdd.net)
  • The Mycobacterium tuberculosis exported repetitive protein (RvErp) is a crucial virulence-associated factor as determined by its role in the survival and multiplication of mycobacteria in cultured macrophages and in vivo Although attempts have been made to understand the function of Erp protein, its exact role in Mycobacterium pathogenesis is still elusive. (osdd.net)
  • Tuberculosis, caused by Mycobacterium tuberculosis, is a highly infectious disease. (wpi.edu)
  • Therefore, immediate discovery of new antibiotic compounds is crucial for counteracting the evolving antibiotic resistance in strains of Mycobacterium tuberculosis and related species. (smu.ca)
  • In previous studies, liposomes obtained from lipids of Mycobacterium smegmatis (LMs) demonstrated their immunogenicity and protective capacity against Mycobacterium tuberculosis in mice. (who.int)
  • Sutherlandia is prescribed for people with tuberculosis but it is still not known what compounds in this plant act against Mycobacterium tuberculosis and its mode of action. (satnt.ac.za)
  • these genes are related to M. smegmatis genes Msmeg_0055-0058 as indicated by their colours. (nih.gov)
  • MSMEG_6441 (Mycobacterium smegmatis str. (nih.gov)
  • A mutant of M. smegmatis lacking egtD ( MSMEG_6247 ) was unable to synthesize ERG, confirming its role in ERG biosynthesis. (asm.org)
  • Mycobacterium smegmatis (MSMEG) is an environmental, nonpathogenic Mycobacterium that is widely used as a model organism to study mycobacterial metabolism and pathogenicity. (asm.org)
  • The Erp protein of Mycobacterium smegmatis (MSMEG_6405, or MsErp) interacts neither with Rv2212 nor with Ms_4279, the M. smegmatis homologue of Rv2212. (osdd.net)
  • This organism was later named M. smegmatis. (redorbit.com)
  • As any immunotherapy involving administration of live BCG organisms (wild-type or recombinant) is likely to have associated toxicity (notably in the immunocompromised), we examined the anti-tumour potential of the closely related nonpathogenic organism, Mycobacterium smegmatis, and a TNFalpha gene-modified recombinant M. smegmatis. (nih.gov)
  • Two possible candidates that could be responsible for maintaining high DNA fidelity in this MMR-deficient organism are the ancestral-like DNA polymerase DnaE1, which contains a highly efficient DNA proofreading histidinol phosphatase (PHP) domain, and/or the existence of a uracil-DNA glycosylase B (UdgB) homolog that might protect the GC-rich M. smegmatis genome against DNA damage arising from oxidation or deamination. (g3journal.org)
  • However, since Mycobacterium is a slower growing organism, the differences between the 5-day and the 7-day biofilms are difficult to ascertain. (asmscience.org)
  • Mycobacterium smegmatis is 3.0 to 5.0 µm long with a bacillus shape, an acid-fast bacterial species in the phylum Actinobacteria. (redorbit.com)
  • M. smegmatis is useful for the research analysis of other Mycobacteria species in laboratory experiments. (redorbit.com)
  • Mycobacterium smegmatis is an acid-fast bacterial species in the phylum Actinobacteria and the genus Mycobacterium. (wikipedia.org)
  • The time and heavy infrastructure needed to work with pathogenic species prompted researchers to use M. smegmatis as a model for mycobacterial species. (wikipedia.org)
  • This suggests that transformation in M. smegmatis is a DNA repair process, presumably a recombinational repair process, as it is in other bacterial species. (wikipedia.org)
  • I. Species' descriptions of Mycobacterium phlei Lehmann and Neumann and Mycobacterium smegmatis (Trevisan) Lehmann and Neumann. (atcc.org)
  • None of 13 other species of Mycobacterium tested by PCR with two sets of primers specific for IS 1549 were positive for the expected amplified product. (asm.org)
  • The genus Mycobacterium is composed of species with widely differing growth rates ranging from approximately three hours in Mycobacterium smegmatis to two weeks in Mycobacterium leprae. (epfl.ch)
  • The HIV pandemic raised the potential for facultative-pathogenic mycobacterial species like, Mycobacterium kansasii, to cause disseminating disease in humans with immune deficiencies. (www.gov.uk)
  • In contrast, non-pathogenic mycobacterial species, like M. smegmatis, are not known to cause disseminating disease even in immunocompromised individuals. (www.gov.uk)
  • A comparison of two rapid-growing, non-pathogenic species (M. smegmatis and M. fortuitum) with two facultative-pathogenic species (M. kansasii and M. bovis BCG) demonstrated that only the non-pathogenic bacteria induced strong apoptosis in human THP-1 cells and murine bone marrow-derived macrophages (BMDM) and dendritic cells (BMDD). (www.gov.uk)
  • Several species of Mycobacteria can cause diseases in animals and humans. (asmscience.org)
  • Since mycolic acid biosynthesis was affected, we cloned and expressed Mycobacterium smegmatis mycolic acid methyltransferases, postulated as targets for thiacetazone in other mycobacterial species. (microbiologyresearch.org)
  • In mycobacteria, these regulators are encoded by an operon, whereas in other bacterial species, SmtB and Zur are encoded on separate loci. (asm.org)
  • We show here, using Mycobacterium smegmatis as a model species, that a significant proportion of these ICDSs result from sequencing errors. (biomedcentral.com)
  • We used M. smegmatis mc 2 155 as the model species for this study. (biomedcentral.com)
  • The Actinomycetes, such as Mycobacterium and Corynebacterium species do not produce GSH and utilize instead mycothiol (MSH) as their alternative LMW thiol. (uni-greifswald.de)
  • 7 days after the last exposure to able to infection by rapidly growing idly growing Mycobacterium species by sick or dead poultry ( 1-4 ). (cdc.gov)
  • The complete genome of M. smegmatis has been sequenced by TIGR, and microarrays have been produced by PFGRC program (http://pfgrc.tigr.org/descriptionPages.shtml), adding further to its use as a model system to study mycobacteria. (wikipedia.org)
  • In addition to esx-1 genes, our screen identifies novel non-esx-1 loci in the M. smegmatis genome that are required for both DNA transfer and ESX-1 activity. (nih.gov)
  • Here, we identify two paralogous CRPs in the genome of Mycobacterium smegmatis that have 78% identical sequences and characterize them biochemically and functionally. (sigmaaldrich.com)
  • Conclusion: PitA is the only low-affinity phosphate transport system annotated in the genome of M. smegmatis. (uni-muenchen.de)
  • A transposon mutant collection with an estimated 1.1-fold genome coverage (7680 mutants) was constructed in Mycobacterium smegmatis and screened in high-throughput against isoniazid. (nih.gov)
  • Mycobacteria have a GC-rich genome and are slow growing and difficult to lyse because of their lipid-rich cell wall. (asm.org)
  • Mutation accumulation experiments of M. smegmatis yielded a base-substitution mutation rate of 5.27 × 10 −10 per site per generation, or 0.0036 per genome per generation, which is surprisingly similar to the mutation rate in MMR-functional unicellular organisms. (g3journal.org)
  • Previous work has reported the impact of VapC upon each gene in the M. smegmatis genome and produced a hypothesis model for the specific motif targeted by the enzyme. (waikato.ac.nz)
  • I found 26 transcriptional regulators that mediated the global transcriptomic response of Mycobacterium smegmatis to nitrogen limitation and I identified several non-coding RNAs that might be involved in the regulation of nitrogen- regulated gene expression. (otago.ac.nz)
  • Convit, J.: Electron microscope study of Mycobacterium leprae and its environment in a vesicular leprous lesion. (springer.com)
  • U07955 Mycobacterium smegmatis ATCC 607 intergenic region between 16S and 23S rRNA genes. (atcc.org)
  • Only the Δ phoU1 Δ phoU2 mutant exhibited constitutive activation of all the RegX3-regulated genes examined, suggesting that M. smegmatis PhoU1 and PhoU2 have overlapping functions in inhibiting activity of the SenX3-RegX3 two-component system when phosphate is readily available. (frontiersin.org)
  • In Middlebrook broth, the strong growth inhibitory effect against M. smegmatis was observed by PNAs targeting the inhA and rpsL genes at all four concentrations. (scirp.org)
  • The PNAs targeting the pncA , polA and rpoC genes were found to exhibit strong growth inhibition against M. smegmatis but only at 20 μM concentration. (scirp.org)
  • To the best of our knowledge, no study has been performed so far to evaluate the growth inhibitory functions of anti-sense PNAs against Mycobacterium targeting one of its essential genes inside the macrophage. (scirp.org)
  • Here we show that the phnDCE genes are common among environmental mycobacteria, where they are often associated with phnF-like genes. (nih.gov)
  • In contrast, pathogenic mycobacteria were not found to encode Phn-like systems but instead were found to possess multiple copies of the pst genes. (nih.gov)
  • Four genes are involved in MSH biosynthesis in mycobacteria, namely, mshA , mshB , mshC , and mshD , and mutants harboring deletions in mshB , mshC , and mshD produce different levels of MSH due to the ability of other enzymes to partially compensate for their loss ( 6 , 7 ). (asm.org)
  • ERG biosynthetic genes ( egtA , egtB , egtC , egtD , and egtE ) were recently identified in M. smegmatis ( 11 ). (asm.org)
  • We have demonstrated that GlnR is the global nitrogen response regulator in M. smegmatis, directly regulating the expression of more than 100 genes. (biomedcentral.com)
  • A number of essential genes have been identified in mycobacteria, but methods to study these genes have not been developed, leaving us unable to determine the function or biology of the genes. (asm.org)
  • Thus, methods for studying essential genes in mycobacteria are urgently required. (asm.org)
  • In M. smegmatis , the expression of msmeg0615 and msmeg0620 genes is broadly similar in differing growth phases and in stress conditions, with the exception of acid stress (pH 4.2). (beds.ac.uk)
  • Analysis of the genomic sequence of Mycobacterium smegmatis suggests the presence of several putative thiolase genes. (iisc.ac.in)
  • We have recently shown that PAF C-16 can directly inhibit the growth of two representative non-pathogenic mycobacteria, Mycobacterium bovis BCG and Mycobacterium smegmatis ( M. smegmatis ), by damaging the bacterial cell membrane. (frontiersin.org)
  • The current vaccine, Mycobacterium bovis Bacillus Calmette-Guerin (BCG) is the most widely used in humans. (sld.cu)
  • The phosphatase activity in whole cells of Mycobacterium bovis BCG was significantly less than that in lysed cells, indicating a similar uptake pathway for phosphates in slow-growing mycobacteria. (uab.edu)
  • 10. Sprott GD, Dicaire CJ, Gurnani K, Sad S, Krishnan L. Activation of dendritic cells by liposomes prepared from phosphatidylinositol mannosides from Mycobacterium bovis bacillus Calmette‑Guerin and adjuvant activity in vivo. (who.int)
  • Mycobacterium smegmatis possesses two such transporters, the widely distributed PstSCAB system and PhnDCE, a transporter that in other bacteria mediates the uptake of alternative phosphorus sources. (nih.gov)
  • Molecular techniques have been at the forefront of the methods for studying bacteria, and the mycobacteria are no exception. (asm.org)
  • These problems partially explain why the development of genetic systems for mycobacteria has lagged behind the development of genetic systems for other bacteria. (asm.org)
  • Mycobacterium smegmatis are aerobic nonmotile bacteria, which are characteristically acid fast and grow well as biofilms (6). (asmscience.org)
  • We performed co-culture experiments using marine-derived Aspergillus niger with Mycobacterium smegmatis, a mycolic acid-containing bacteria. (olmdiagnostics.com)
  • inhA (a fatty acid elongase), rpsL (ribosomal S12 protein), gyrA (DNA gyrase), pncA (pyrazinamidase), polA (DNA polymerase I) and rpoC (RNA polymerase β subunit) of M. smegmatis . (scirp.org)
  • In this study, we evaluated a recombinant vaccine prepared from non-pathogenic Mycobacterium smegmatis (rMS) that expresses a fusion of early secreted antigenic target 6-kDa antigen (ESAT6) and culture filtrate protein 10 (CFP10). (semanticscholar.org)
  • IL-17A Autoantibody Induced by Recombinant Mycobacterium smegmatis Expressing Ag85A-IL-17A Fusion Protein. (semanticscholar.org)
  • Interaction between FtsW and penicillin-binding protein 3 (PBP3) directs PBP3 to mid-cell, controls cell septation and mediates the formation of a trimeric complex involving FtsZ, FtsW and PBP3 in mycobacteria. (microbiologyresearch.org)
  • Summary: Treatment of growing Mycobacterium smegmatis with hydroxyurea decreased the DNA/protein ratio and markedly increased the specific activities of DNA polymerase and ATP-dependent deoxyribonuclease. (microbiologyresearch.org)
  • Treating M. smegmatis with nalidixic acid also decreased the DNA/protein ratio but had no effect on the activity of either enzyme. (microbiologyresearch.org)
  • A similar result was obtained for M. smegmatis that overexpressed endogenous alkaline phosphatase, indicating that PhoA is not a surface protein, contrary to a previous report. (uab.edu)
  • For the M. smegmatis enzyme, the Vmax for S-adenosylmethionine formation was 1.30 μmol/min/mg protein and the Km for methionine and ATP was 288 μM and 76 μM respectively. (beds.ac.uk)
  • Here, the cloning, expression, purification and crystallization of this putative SCP-x protein from M. smegmatis are reported. (iisc.ac.in)
  • Paralogous cAMP receptor proteins in Mycobacterium smegmatis show biochemical and functional divergence. (sigmaaldrich.com)
  • For each proteins, the regions containing T and B epitopes were selected at the same time to look for identical epitopes on M. smegmatis based on sequence alignments. (sld.cu)
  • The role of FtsZ-associated proteins in the regulation of the assembly dynamics of Mycobacterium smegmatis FtsZ is not clear. (biochemj.org)
  • X60513 M.smegmatis gltA gene for citrate synthase. (atcc.org)
  • U07954 Mycobacterium smegmatis ATCC 607 16S rRNA gene, 5' untranslated region. (atcc.org)
  • U87307 Mycobacterium smegmatis extracytoplasmic function alternative sigma factor (sigE) gene, complete cds. (atcc.org)
  • By using the rapid-growing Mycobacterium smegmatis as a model genetic system, a gene was selected that confers low-level FQ resistance when present on a multicopy plasmid. (pnas.org)
  • A new insertion element, IS 1549 , was identified serendipitously from Mycobacterium smegmatis LR222 during experiments using a vector designed to detect the excision of IS 6110 from between the promoter region and open reading frame (ORF) of an aminoglycoside phosphotransferase gene. (asm.org)
  • Only strains with a 65-kDa antigen gene with a PCR-restriction fragment length polymorphism type identical to that of M. smegmatis 607 contain IS 1549 . (asm.org)
  • A previous study showed antisense PNA targeting only inh A gene inhibited the growth of M . smegmatis in pure culture [9]. (scirp.org)
  • In addition, all mycobacteria also contain at least one gene encoding the low-affinity phosphate transporter, Pit. (uni-muenchen.de)
  • The involvement of GlgE in glycogen biosynthesis was discovered twenty years ago when the phenotype of a temperature-sensitive Mycobacterium smegmatis mutation was rescued by the glgE gene. (jic.ac.uk)
  • Using a reporter gene which encodes an unstable variant of GFP, we showed that tetracycline-inducible expression occurred in M. smegmatis and that expression levels were titratable to some extent by varying the concentration of tetracycline. (asm.org)
  • Using an inducible mycobacterial shuttle vector, we have identified three ORFs within an early operon of mycobacteriophage L5 which encode gene products (gp) toxic to the host M. smegmatis when expressed. (microbiologyresearch.org)
  • Conditional expression of Mycobacterium smegmatis ftsZ , an essential cell division gene. (microbiologyresearch.org)
  • In further work, I demonstrated that deletion of the gene cadC in M. smegmatis resulted in a severe growth defect manifested as cell lysis during growth on rich medium. (otago.ac.nz)
  • We report here a new plasmid construct designated as pMSuia from pBAD-TOPO that harbors a 1.1 kb phytase gene (phyMS) from Mycobacterium smegmatis, and expression as well as characterization of the purified recombinant M. smegmatis phytase. (iium.edu.my)
  • The global variations in the gene expression pattern of drug treated (½X) and laboratory evolved drug-resistant strains (2XR and 4XR) of Mycobacterium smegmatis were obtained and compared with the M. smegmatis mc2 155 (WT) strain. (ias.ac.in)
  • Some of the Ndh mutants have a T s lethal phenotype, which indicates that Ndh is essential for the viability of M. smegmatis . (asm.org)
  • The growth rate of M. smegmatis dropped drastically with its porin-mediated OM permeability in contrast to porin mutants of Escherichia coli. (sigmaaldrich.com)
  • The ERG- and MSH-deficient double mutant was significantly more sensitive to peroxide than either of the single mutants lacking either ERG or MSH, suggesting that both thiols play a role in protecting M. smegmatis against oxidative stress and that ERG is able to partly compensate for the loss of MSH. (asm.org)
  • Mycobacterium smegmatis alr insertion mutants are not dependent on d-alanine for growth and display a metabolic pattern consistent with an alternative pathway for d-alanine biosynthesis. (unl.edu)
  • Inductively coupled plasma mass spectroscopy (ICP-MS) analysis of wild-type M. smegmatis and energy-driven ion transport mutants revealed the molecular inventory of cations in M. smegmatis under normoxia and hypoxia. (otago.ac.nz)
  • Cellular and humoral immunogenicity of recombinant Mycobacterium smegmatis expressing Ag85B epitopes in mice. (semanticscholar.org)
  • Objective To observe humoral and cellular immune responses induced in mice by a TSOL18 recombinant Mycobacterium smegmatis (MS) vaccine of Taenia solium . (bvsalud.org)
  • Truncated structural variants of lipoarabinomannan in ethambutol drug-resistant strains of Mycobacterium smegmatis. (semanticscholar.org)
  • Mycobacterium smegmatis is a bacterium that is naturally devoid of known postreplicative DNA mismatch repair (MMR) homologs, mutS and mutL , providing an opportunity to investigate how the mutation rate and spectrum has evolved in the absence of a highly conserved primary repair pathway. (g3journal.org)
  • We also found that the transition-mutation rate of M. smegmatis is significantly lower than that of other naturally MMR-devoid or MMR-knockout organisms. (g3journal.org)
  • The mutation features of M. smegmatis provide further evidence that genomes harbor alternative routes for improving replication fidelity, even in the absence of major repair pathways. (g3journal.org)
  • M. smegmatis and M. avium are both opportunistic pathogens (8). (asmscience.org)
  • Our results suggest that M. smegmatis can serve as a tractable model for further characterization of the molecular mechanism of phosphate sensing in mycobacteria and to screen for compounds that would interfere with signal transduction and thereby increase the efficacy of existing anti-tubercular antibiotics. (frontiersin.org)
  • expression in Mycobacterium smegmatis and biochemical characterization. (microbiologyresearch.org)
  • Moreover, the inhibition of phospholipase C and phospholipase A 2 activities, involved in PAF C-16 signaling pathway, increased survival of intracellular M. smegmatis . (frontiersin.org)
  • Use of a number of PAF C-16 structural analogs, including Lyso-PAF, 2-O-methyl PAF, PAF C-18 and Hexanolamino PAF, revealed that the presence of acetyl group (CH 3 CO) at sn -2 position of the glycerol backbone of PAF is important for the intracellular growth inhibition activity against M. smegmatis . (frontiersin.org)
  • No growth inhibition of M. smegmatis was seen in pure culture when treated with PNAs targeting gyrA and a mismatch PNA targeting dnaG (DNA primase). (scirp.org)
  • Non-mutagenic epigallocatechin gallate inhibits the growth of M. smegmatis and warrants further investigation as an adjunct therapy for pathogenic mycobacteria. (ijpsonline.com)
  • Indeed, PI-LAM induces high levels of apoptosis and IL-12 expression compared to the mannosyl modification of LAM isolated from facultative-pathogenic mycobacteria. (www.gov.uk)
  • Consistently, BALB/c BMDM responded by secreting large amounts of TNF upon infection with non-pathogenic but not facultative-pathogenic mycobacteria. (www.gov.uk)
  • Interestingly, C57Bl/6 BMDM do not undergo apoptosis upon infection with non-pathogenic mycobacteria despite the fact that they still induce an increase in TNF secretion. (www.gov.uk)
  • These results demonstrate a much stronger induction of the innate immune response by non-pathogenic versus facultative-pathogenic mycobacteria as measured by host cell apoptosis, IL-12 and TNF cytokine induction. (www.gov.uk)
  • Briken, V. The Non-Pathogenic Mycobacteria M. Smegmatis and M. Fortuitum Induce Rapid Host Cell Apoptosis Via a Caspase-3 and TNF Dependent Pathway. (www.gov.uk)
  • Impairment of D-alanine biosynthesis in Mycobacterium smegmatis " by Ofelia Barletta-Chacon, Luiz E. Bermudez et al. (unl.edu)
  • In the soil dwelling saprophyte Mycobacterium smegmatis the OmpR-type response regulator GlnR is thought to mediate the transcriptomic response to nitrogen limitation. (biomedcentral.com)
  • The overarching aim of this thesis was to characterise ion homeostasis and energetics in the saprophyte Mycobacterium smegmatis, under normoxia and hypoxia, under ion chelation and deficiency, with heavy-metal exposure and in response to anti-tubercular compounds with putative disruptive effects on ion energetics, including bedaquiline and amiloride derivatives. (otago.ac.nz)
  • However, a complex plasmid swapping strategy was required to generate the Δ phoU1 Δ phoU2 mutant, suggesting that either phoU1 or phoU2 is essential for in vitro growth of M. smegmatis . (frontiersin.org)
  • Deletion of egtD from wild-type M. smegmatis and an MSH-deficient mutant did not affect their susceptibility to antibiotics tested in this study. (asm.org)
  • An unmarked deletion mutant in pitA of M. smegmatis was created. (uni-muenchen.de)
  • However, increased expression of the remaining phosphate transporters in the mutant indicates a compensatory mechanism and implies that PitA is indeed used for the uptake of phosphate in M. smegmatis. (uni-muenchen.de)
  • We investigated the role of GlnR in the nitrogen limitation response and determined the entire GlnR regulon , by combining expression profiling of M. smegmatis wild type and glnR deletion mutant, with GlnR-specific chromatin immunoprecipitation and high throughput sequencing. (biomedcentral.com)
  • In this study, we demonstrate that the M. smegmatis alr insertion mutant TAM23 can synthesize d-alanine at lower levels than the parental strain. (unl.edu)
  • Here we show that Mycobacterium smegmatis MmpL3 mutant strains had an altered cell wall hydrophobicity, disrupted membrane potential and growth defects in liquid media. (microbiologyresearch.org)
  • M. smegmatis MmpL3 mutant strains were resistant to two anti-tubercular agents, SQ109 and AU1235, but were more sensitive to rifampicin, erythromycin and ampicillin. (microbiologyresearch.org)
  • Disruption of potassium homeostasis in the ΔtrkA mutant of M. smegmatis resulted in the reduction of (~10-50%) intracellular potassium concentrations and elevated (~10-30%) intracellular sodium concentrations, coupled with reductions in viability and persistence under hypoxia. (otago.ac.nz)
  • My data suggest that a ∆cadC mutant is defective in the generation or replenishment of intracellular lysine and DAP levels that are essential for growth and survival in mycobacteria. (otago.ac.nz)
  • The uptake of phosphate by a mutant lacking the porins MspA and MspC was twofold lower than that by wild-type M. smegmatis. (uab.edu)
  • M. smegmatis is commonly used in work on the Mycobacterium genus due to it being a "fast grower" and non-pathogenic. (wikipedia.org)
  • The M. smegmatis strain is now the work-horse of mycobacterial genetics. (redorbit.com)
  • However, in contrast to the well-known E. coli Hfr conjugation system, in M. smegmatis all regions of the chromosome are transferred with comparable efficiencies and mycobacterial conjugation is chromosome, rather than plasmid based. (wikipedia.org)
  • Background: Mycobacteria have been shown to contain an apparent redundancy of high-affinity phosphate uptake systems, with two to four copies of such systems encoded in all mycobacterial genomes sequenced to date. (uni-muenchen.de)
  • Unlike other bacterial biofilms which synthesize EPS composed predominantly of polysaccharides, mycobacterial EPS contains large amounts of mycolic acids (waxes), which are also found in the cell envelope of mycobacteria. (asmscience.org)
  • Furthermore, it is shown that even though mycobacterial UDG-Ugi complexes dissociate in 5-6 M urea, Ugi is still a potent inhibitor of UDG activity in mycobacteria. (iisc.ernet.in)
  • The deletion did not affect in vitro growth or phosphate uptake of M. smegmatis. (uni-muenchen.de)
  • used genetic manipulation and microscopy to study how mycobacteria build their cell wall. (elifesciences.org)
  • Superinfection immunity of mycobacteriophage L5: applications for genetic transformation of mycobacteria. (microbiologyresearch.org)
  • The HR pathway of M. smegmatis is the major determinant of resistance to ionizing radiation and oxidative DNA damage. (wikipedia.org)
  • NHEJ is the preferred pathway during stationary phase, and it protects M. smegmatis against the harmful effects of desiccation. (wikipedia.org)
  • Arachidonic acid, a downstream metabolite of PAF C-16 signaling pathway, directly inhibited the growth of M. smegmatis in vitro . (frontiersin.org)
  • Background - The bacterial GlgE pathway is the third known route to glycogen and is the only one present in mycobacteria. (jic.ac.uk)
  • General Significance - This study highlights how the lowering of flux through the GlgE pathway can slow the growth mycobacteria. (jic.ac.uk)
  • Nitrogen metabolism in Mycobacterium smegmatis (Thesis, Doctor of Philosophy). (otago.ac.nz)
  • In this thesis, I have developed a nitrogen-limited continuous culture system to gain further insights into nitrogen metabolism in mycobacteria and their response to nitrogen limitation. (otago.ac.nz)
  • I conclude that M. smegmatis has a broad network of regulatory systems that together enable M. smegmatis to adapt its nitrogen metabolism to rapidly changing environments. (otago.ac.nz)
  • However, in mycobacteria, knowledge of zinc metabolism is incomplete. (asm.org)
  • Consequently a faster growing, nonpathogenic Mycobacterium would be ideal as a first screen in drug discovery. (bl.uk)
  • Growth of M. smegmatis under conditions of zinc limitation did not diminish the specific activity of DNA polymerase thus not favouring a role for zinc in the action of this enzyme. (microbiologyresearch.org)
  • The kinetic properties of the M. smegmatis enzyme were also similar to known prokaryotic methionine adenosyltransferases. (beds.ac.uk)
  • Ni-NTA affinity purified recombinant M. smegmatis phytase exhibited specific activity of 233.51 U/mg, optimal pH of 3 and 7 and optimal temperature of 60˚C. The purified enzyme retains almost 30% of the initial activity after incubation at 90˚C for 60 min. (iium.edu.my)
  • Here, by infecting THP-1 macrophages with Ms_Rv2820c-Bj, Ms_Rv2820c and MS_Rv2820c-Tr (expressing the truncated Rv2820c without five amino acid mutations at 3′-end), we found only Ms_Rv2820c-Bj was responsible for the enhancement of survival of M. smegmatis in macrophages. (deepdyve.com)
  • Interrupted coding sequences in Mycobacterium smegmatis : authentic mutations or sequencing errors? (biomedcentral.com)
  • Addgene: The Phn system of Mycobacterium smegmatis: a second high-affinity ABC-transporter for phosphate. (addgene.org)
  • Furthermore, I showed that M. smegmatis has a high-affinity lysine uptake system that exhibited high rates of lysine transport during growth in minimal medium, which was significantly reduced during growth in rich medium. (otago.ac.nz)
  • The present study was directed at investigating the role of the low-affinity phosphate transporter in a bacterium containing at least two high-affinity systems, using the model of M. smegmatis . (biomedcentral.com)
  • M. smegmatis relies on DNA repair pathways to resist DNA damage. (wikipedia.org)
  • The M. smegmatis PrrAB two-component system regulates respiratory and oxidative phosphorylation pathways, potentially to provide tolerance against the dynamic environmental conditions experienced in its natural ecological niche. (biomedcentral.com)
  • Mycobacteria are part of the native microflora found in soils and aquatic environments and harbour an extensive repertoire of uptake and efflux and secretion pathways to modulate ions and metabolites including, sodium, potassium, calcium, magnesium, iron, nickel, manganese, zinc, copper and cobalt. (otago.ac.nz)
  • There remains a paucity of information regarding ion homeostasis networks and pathways in mycobacteria. (otago.ac.nz)
  • The growth rate of Mycobacterium smegmatis depends on sufficient porin-mediated influx of nutrients. (sigmaaldrich.com)
  • These results show that porin-mediated influx of nutrients is a major determinant of the growth rate of M. smegmatis. (sigmaaldrich.com)
  • Here, we have examined the effect of PAF C-16 on M. smegmatis residing within macrophages, and identified mechanisms involved in their growth inhibitory function. (frontiersin.org)
  • Taken together, these results suggest that exogenous PAF C-16 treatment inhibits intracellular M. smegmatis growth, at least partially, in a nitric oxide and TNF-α dependent manner. (frontiersin.org)
  • A study was carried out to determine the efficacy of several antisense PNAs in inhibiting extracellular and intracellular growth of Mycobacterium smegmatis . (scirp.org)
  • In this study, quantification of ERG at various points in the growth cycle of Mycobacterium smegmatis revealed that a significant portion of ERG is found in the culture media, suggesting that it is actively secreted. (asm.org)
  • Strikingly, the loss of these porins resulted in a severe growth defect of M. smegmatis on low-phosphate plates. (uab.edu)
  • Also, the biological significance of the interaction between RvErp and Rv2212 was demonstrated by the enhanced survival of M. smegmatis within THP-1 macrophages. (osdd.net)
  • Strains of M. smegmatis that have particularly efficient DNA repair machinery, as indicated by their greater resistance to the DNA damaging effects of agents such as UV and mitomycin C, proved to be the most capable of undergoing transformation. (wikipedia.org)
  • In this report, we describe a new mechanism for INH resistance in Mycobacterium smegmatis . (asm.org)
  • Overexpression of nudC, as well as inhA which encodes a known target of isoniazid, increased M. smegmatis resistance to isoniazid, but failed to increase resistance to three of the analogues, NSC27607, NSC33759, and NSC40350. (nih.gov)
  • Conjugal DNA transfer in M. smegmatis requires stable and extended contact between a donor and a recipient strain, is DNase resistant, and the transferred DNA is incorporated into the recipient's chromosome by homologous recombination. (wikipedia.org)
  • A detailed biochemical analysis of PhnF binding to its identified binding sites in the phnD-phnF intergenic region of M. smegmatis has allowed us to propose a quantitative model for repressor binding, which shows that a PhnF dimer binds independently to each site. (nih.gov)
  • Collectively, our data demonstrate that the two M. smegmatis PhoU orthologs have overlapping functions in both controlling SenX3-RegX3 activity in response to phosphate availability and regulating phosphate transport by the Pst system. (frontiersin.org)
  • The specialized secretory apparatus ESX-1 is essential for DNA transfer in Mycobacterium smegmatis. (nih.gov)
  • This also indicated that a minimal porin-mediated OM permeability is essential for survival of M. smegmatis. (sigmaaldrich.com)
  • This study has demonstrated the molecular inventory of intracellular ions in M. smegmatis under several key laboratory conditions and has characterised essential components of ion homeostasis and more specifically, potassium homeostasis and energetics in mycobacteria. (otago.ac.nz)
  • This study explores the potential use of computational models to characterize the sites targeted by VapC in Mycobacterium smegmatis. (waikato.ac.nz)
  • Epigallocatechin gallate, a green tea catechin with significant antimicrobial properties, and its derivatives have been studied for their inhibitory effects on Mycobacterium smegmatis . (ijpsonline.com)
  • Conjugal DNA transfer in Mycobacterium smegmatis occurs by a mechanism distinct from plasmid-mediated DNA transfer. (nih.gov)
  • SmtB and Zur are important antagonistically acting bacterial regulators in mycobacteria. (asm.org)
  • To date, the acetamide-inducible system of Mycobacterium smegmatis has been the most widely used controllable promoter in mycobacteria ( 7 , 20 ). (asm.org)
  • When wild-type M. smegmatis were delivered to immunocompetent C57Bl/6 mice bearing the transplantable MB49 bladder tumour, efficacy comparable to live BCG was observed with 10-20% long-term survival. (nih.gov)
  • Recombinant M. smegmatis secreting TNFalpha, however, gave a 70% durable tumour-free survival. (nih.gov)
  • Accordingly, we concluded that the new 3′-end of Rv2820c-Bj was important to dampen host defense and enhance the intracellular survival of M. smegmatis. (deepdyve.com)
  • Mycobacterium smegmatis ist eine Art in der Gattung Mycobacterium in der Familie der Mycobacteriaceae , die zur Abteilung Actinobacteria gezählt wird. (eol.org)