A technique encompassing morphometry, densitometry, neural networks, and expert systems that has numerous clinical and research applications and is particularly useful in anatomic pathology for the study of malignant lesions. The most common current application of image cytometry is for DNA analysis, followed by quantitation of immunohistochemical staining.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
The degree of replication of the chromosome set in the karyotype.
Methods for using more than one primer set in a polymerase chain reaction to amplify more than one segment of the target DNA sequence in a single reaction.
The chromosomal constitution of cells which deviate from the normal by the addition or subtraction of CHROMOSOMES, chromosome pairs, or chromosome fragments. In a normally diploid cell (DIPLOIDY) the loss of a chromosome pair is termed nullisomy (symbol: 2N-2), the loss of a single chromosome is MONOSOMY (symbol: 2N-1), the addition of a chromosome pair is tetrasomy (symbol: 2N+2), the addition of a single chromosome is TRISOMY (symbol: 2N+1).
DNA present in neoplastic tissue.
A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.
A technique of inputting two-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
Small uniformly-sized spherical particles, of micrometer dimensions, frequently labeled with radioisotopes or various reagents acting as tags or markers.
The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
The application of genetic analyses and MOLECULAR DIAGNOSTIC TECHNIQUES to legal matters and crime analysis.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
A country consisting of the eastern half of the island of New Guinea and adjacent islands, including New Britain, New Ireland, the Admiralty Islands, and New Hanover in the Bismarck Archipelago; Bougainville and Buka in the northern Solomon Islands; the D'Entrecasteaux and Trobriand Islands; Woodlark (Murua) Island; and the Louisiade Archipelago. It became independent on September 16, 1975. Formerly, the southern part was the Australian Territory of Papua, and the northern part was the UN Trust Territory of New Guinea, administered by Australia. They were administratively merged in 1949 and named Papua and New Guinea, and renamed Papua New Guinea in 1971.
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
MOLECULAR BIOLOGY techniques used in the diagnosis of disease.
The study of the structure, growth, function, genetics, and reproduction of viruses, and VIRUS DISEASES.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
A scanning microscope-based, cytofluorimetry technique for making fluorescence measurements and topographic analysis on individual cells. Lasers are used to excite fluorochromes in labeled cellular specimens. Fluorescence is detected in multiple discrete wavelengths and the locational data is processed to quantitatively assess APOPTOSIS; PLOIDIES; cell proliferation; GENE EXPRESSION; PROTEIN TRANSPORT; and other cellular processes.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Discussions, descriptions or catalogs of public displays or items representative of a given subject.
Transmission of live or pre-recorded audio or video content via connection or download from the INTERNET.
Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)
Laws and regulations pertaining to the field of dentistry, proposed for enactment or recently enacted by a legislative body.
The principles of proper professional conduct concerning the rights and duties of the dentist, relations with patients and fellow practitioners, as well as actions of the dentist in patient care and interpersonal relations with patient families. (From Stedman, 25th ed)
Time period from 1801 through 1900 of the common era.
Individuals responsible for fabrication of dental appliances.
The educational process of instructing.
Assistants to a veterinarian, biological or biomedical researcher, or other scientist who are engaged in the care and management of animals, and who are trained in basic principles of animal life processes and routine laboratory and animal health care procedures. (Facts on File Dictionary of Health Care Management, 1988)
An adrenocortical steroid that has modest but significant activities as a mineralocorticoid and a glucocorticoid. (From Goodman and Gilman's The Pharmacological Basis of Therapeutics, 8th ed, p1437)
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Organic or inorganic compounds that contain the -N3 group.
Antibodies produced by a single clone of cells.
A genus in the family ORTHOMYXOVIRIDAE causing influenza and other diseases in humans and animals. It contains many strains as well as antigenic subtypes of the integral membrane proteins hemagglutinin (HEMAGGLUTININS) and NEURAMINIDASE. The type species is INFLUENZA A VIRUS.
A cytochrome oxidase inhibitor which is a nitridizing agent and an inhibitor of terminal oxidation. (From Merck Index, 12th ed)
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Proteins prepared by recombinant DNA technology.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Large collections of small molecules (molecular weight about 600 or less), of similar or diverse nature which are used for high-throughput screening analysis of the gene function, protein interaction, cellular processing, biochemical pathways, or other chemical interactions.
Tissue, organ, or gamete donation intended for a designated recipient.
A technology, in which sets of reactions for solution or solid-phase synthesis, is used to create molecular libraries for analysis of compounds on a large scale.
Rapid methods of measuring the effects of an agent in a biological or chemical assay. The assay usually involves some form of automation or a way to conduct multiple assays at the same time using sample arrays.
Preclinical testing of drugs in experimental animals or in vitro for their biological and toxic effects and potential clinical applications.
The process of finding chemicals for potential therapeutic use.
Communication between animals involving the giving off by one individual of some chemical or physical signal, that, on being received by another, influences its behavior.
Flow-FISH uses flow cytometry to perform FISH automatically using per-cell fluorescence measurements. Microfluidics-assisted ... Separate but compatible signal amplification systems enable the multiplex assay (up to two targets per assay). Signal ... Single-molecule RNA FISH assays can be performed in simplex or multiplex, and can be used as a follow-up experiment to ... or imaged simultaneously with a fluorescent antibody assay. The technology has potential applications in cancer diagnosis, ...
... optic Flow cytometric cell or particle counters, or coulter/impedance principle based cell counters Imaging assays, that ... Usual assays are simple or single target assays which is usually the default unless it is called multiplex. Multiplex assays ... involve image analysis manually or by software: Cytometry: When the size statistics of cells is assessed by an image processor ... DNase footprinting assay Filter binding assay Gel shift assay Bicinchoninic acid assay (BCA assay) Bradford protein assay Lowry ...
"Standardization of cytokine flow cytometry assays". BMC Immunology. 6: 13. doi:10.1186/1471-2172-6-13. PMC 1184077. PMID ... "Multiplexed mass cytometry profiling of cellular states perturbed by small-molecule regulators". Nature Biotechnology. 30 (9): ... Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI. 6859: 17. Bibcode:2008SPIE.6859E..17L. CiteSeerX ... coupled with the development of high-throughput robotic platforms capable of assaying hundreds to thousands of samples ...
... stemming off of the work done to sort cells in Flow Cytometry. Within the realm of droplet sorting there are two main types, ... This multiplexing was made possible with immobilized DNA primer fragments placed in the base of the individual wells of the ... Gu S, Lu Y, Ding Y, Li L, Zhang F, Wu Q (September 2013). "Droplet-based microfluidics for dose-response assay of enzyme ... January 2017). "An on-chip imaging droplet-sorting system: a real-time shape recognition method to screen target cells in ...
ViewRNA assay for CTCs characterization is the only in situ hybridization technology that allows multiplex, single-molecule RNA ... enrichment-free assays that identify all CTC subtypes in sufficiently high definition to satisfy diagnostic pathology image- ... Cytometry Part A. 71A (3): 132-142. doi:10.1002/cyto.a.20369. PMID 17200956.. ... "Computational Analysis of Microfluidic Immunomagnetic Rare Cell Separation from a Particulate Blood Flow". Analytical ...
... flow cytometry, and molecular-pathologic analysis.[9] One of the greatest challenges of dermatopathology is its scope. More ... multiplex PCR, DNA microarray, in situ hybridization, DNA sequencing, antibody based immunofluorescence tissue assays, ... Examples of important subdivisions in medical imaging include radiology (which uses the imaging technologies of X-ray ... tactile imaging, thermography, medical photography, nuclear medicine and functional imaging techniques such as positron ...
Microfluidic flow enables fast sample throughput, automated imaging of large sample populations, as well as 3D capabilities. or ... "Simultaneous Acoustic and Photoacoustic Microfluidic Flow Cytometry for Label-free Analysis". Sci. Rep. 9 (1): 1585. Bibcode: ... Uram, J. D.; Ke, K.; Hunt, A. J.; Mayer, M. (2006). "Label-free affinity assays by rapid detection of immune complexes in ... Yetisen AK, Jiang L, Cooper JR, Qin Y, Palanivelu R, Zohar Y (May 2011). "A microsystem-based assay for studying pollen tube ...
... which display defined fluorescence properties and can be identified using standard flow cytometry techniques. , USA ... MACSPlex Cytokine Assays are designed for determining concentrations of up to 7 soluble analytes in a single sample. The ... The MACSPlex Cytokine Basic Kit contains the supplementary components necessary to perform a MACSPlex Cytokine assay. It has to ... Antibodies for flow cytometry. *Antibodies for microscopy and imaging. *Antibodies for functional assays ...
We offer microplate assays for whole cells and assays performed on disrupted cells or cell lysates. Metabolic activity is ... Microplate assays provide information on entire cell populations rather than tracking the behavior of individual cells. ... Also see imaging assays for cell viability. *Also see flow cytometry assays for cell viability. ... Our colorimetric viability assays the Vybrant MTT assay and CyQUANT XTT assay include all the reagents necessary to generate ...
An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry… ... Everley, R. A., Kunz, R. C., McAllister, F. E., Gygi, S. P. Increasing Throughput in Targeted Proteomics Assays: 54-Plex ... Gu, L., Evans, A. R., Robinson, R. A. S. Sample Multiplexing with Cysteine-Selective Approaches: cysDML and cPILOT. J. Am. Soc ... Hebert, A. S., et al. Neutron-encoded mass signatures for multiplexed proteome quantification. Nat Meth. 10, (4), 332-334 (2013 ...
Quantitative immunoassays and multiplex assays. *Immunology and flow cytometry. About Abcam. As an innovator in reagents and ... considerable expertise in multiple technologies commonly used in molecular biology such as cellular imaging and flow cytometry. ... Prior to joining Abcam she held various positions at EMD-Millipore, latterly as R&D Director, leading the Antibody and Assay ... An Introduction to Flow Cytometry with Troubleshooting... Speaker: Ina Schulte, PhD Presented At: Abcam virtual exhibition: ...
Flow Cytometry-Based Assays.. Control or YAPS127A-expressing cells that also expressed either mCherry or ZSgreen were mixed in ... 105 cells of the indicated mixtures were assayed by tail-vein metastasis assay. (Left) Fluorescent images of whole lungs after ... 3 and 4). Luminex-based assays also can be used to multiplex in vitro assays for transformation and cell proliferation (Fig. 5 ... The percentages of red and green cells were quantified using the LSR II flow cytometer (BD). In all flow cytometry-based ...
... which is powerful enough to run complex assays. ... Flow Cytometry and Imaging. Luminex flow cytometers and ... easyCyte Flow Cytometers use novel microcapillary technology that enables a compact user-friendly format, ... Performance of a High Sensitivity Multiplexed Assay for Malarial Antigen Detection on Multiple Cytometry Platforms - Poster ... Guava® easyCyte™ Systems - Expanding the Potential of Flow Cytometry. Flexible, intuitive, and affordable cytometry for all ...
Biochemical Assays * Cellular Assays * ELISpot * Flow Cytometry Kits * Independently validated ELISA Kits ... This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. ... ELISA & Assay Kits * antibodies-online™ ELISA Kits * ELISA Kits * Annexin V-FITC Apoptosis Detection Kits ... Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and ...
Multiplexed cellular readouts - Flow cytometry, fluorescence microscopy including live imaging of tissue sections. ... To better model how IO drugs interact with a patients cancer cells and immune system, we have developed an in vitro assay ... Biochemical assays - protein analysis, cell viability readouts, antibody conjugation.. *Molecular biology - mRNA extraction, ... Specifically, use of a unique in vitro assay system, the tissue slice culture, will be central to the role. ...
This product will provide 200 assay if using flow cytometry or 300 assays for fluorescent imaging.. For additional protocol ... Multiplex for cell death with FLICA and DRAQ7. Jurkat cells were treated with either a placebo or staurosporine for 4 hours. ... Using Flow Cytometry to Quantify Immune Response and Cell-Mediated Cytotoxicity from Drug Treatments and Viral Infections ... Home / Cell Viability Assays / Cellular Imaging / DRAQ7 Far-Red Live-Cell Impermeant DNA Dye. ...
Cell images were acquired with imaging flow cytometry.By means of imaging flow cytometry, we demonstrate a strong and quick ... We used bead-based multiplex assays and quantitative PCR for cytokine quantification. HRV accumulation and replication inside ... A novel, dual cytokine-secretion assay for the purification of human Th22 cells that do not co-produce IL-17A. Allergy ... The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated ...
High-Throughput Flow Cytometry Bead-Based Multiplex Assay for Identification of Rho GTPase Inhibitors ... Biochemical Assays to Characterize Rho GTPases Mamta Jaiswal, Badri N. Dubey, Katja T. Koessmeier, Lothar Gremer, Mohammad R. ... FRET-Based Imaging of Rac and Cdc42 Activation During Fc-Receptor-Mediated Phagocytosis in Macrophages ... Quantitative and Robust Assay to Measure Cell-Cell Contact Assembly and Maintenance ...
Flow cytometry. For staining the following culture, cells were harvested and transferred onto V-bottomed 96-well plates. Cells ... We performed a fluorescence-based multiplex immunohistochemistry (IHC) assay, as previously described (19), with slight ... Ten-fold dilution series of these assays on monocyte cDNA showed no alteration in efficiency with assays performed in single- ... In merged image panels, colocalization of VISTA (green) and CD11b (magenta) signal seems white. A consecutive tissue slide was ...
... immunological assays such as commercial or customized ELISA, multiplexed bead array (Luminex), NK cell assay, total and ... flow cytometry; luminescent imaging using mice with reporter genes (NF-kappaB); microarray analysis (transcriptomics); ... specific antibodies, differential cell counts; general assays for, apoptosis; cellular viability; cell cycle analysis; cellular ...
Multiplexed binding assays (96-well plate in 5 min). *Identify targets in their native conformation ... Get information on cell health, isotype and titer from a single assay ... Visit the Sartorius booth #B5-6, at Elrig Flow Cytometry & Imaging 2018, to learn the latest on the IncuCyte® S3 Live-Cell ... Powerful imaging & analysis tools for real-time decision-making. Also showcased is the Intellicyt iQue Screener PLUS. Discover ...
... facilitation of seamless downstream assaying in 96-well format plate reader, imaging, and flow cytometry experiments. ... such an approach minimizes costs per sample and handsontime and maximizes results by multiplexing different assays from the ... Essentially, the combination of multiplate reader, microscopy, and flow cytometry multiplexes information on cellular responses ... many other questions can be addressed with this approach linking metabolic activity with imaging and flow cytometry results. ...
Core personnel are continuously working with clients to develop new assays or multiplex existing assays to satisfy user ... Current instrumentation allows simultaneous quantitative analysis of up to 12 parameters, each of which is assayed at the ... Flow Cytometry. Use of functional dyes as well as antibody-coupled fluorophores provides indispensable tools for the study of ... While similar to traditional fluorescence based flow cytometry the use of metal labels removes the need for compensation and ...
... allowing researchers to multiplex immunology markers in a single assay on a single slide and really get at the picture of how ... "And image analysis…can look at morphometry, can look at biomarker distribution, it can look at many different things. And we ... developing the assays, understanding what matters and what doesnt matter." ... throughput in flow cytometry experiments is reportedly increased by tenfold to twentyfold over other plate-based flow cytometry ...
... functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. ... Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.. Notes. ICC. Use at an assay dependent ... Multiplex miRNA assays. Multiplex Assays. By research area. Cancer. Cardiovascular. Cell Biology. Epigenetics. Metabolism. ... Cell and tissue imaging tools. Cellular and biochemical assays. Proteins and Peptides. By product type. Proteomics tools. ...
Validated in WB, IP, ICC/IF, Flow Cyt (Intra) and tested in Mouse, Rat, Human. ... functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. ... Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 86 kDa). ... Multiplex miRNA assays. Multiplex Assays. By research area. Cancer. Cardiovascular. Cell Biology. Epigenetics. Metabolism. ...
Solicitation of Assays for High Throughput Screening (HTS) in the Molecular Libraries Probe Production Centers Network (MLPCN ... multiplexed flow cytometry, ion channel assays, and biocontainment assays) may not fit well in the comprehensive center format ... protein binding assay, RT-PCR and Western assay vs. reporter gene assay, cell based assay vs. biochemical assay, etc.); ... Assays that require long-time kinetic recordings, high content imaging, multiple washing and aspiration steps, whole organism ...
These flexible assays are compatible with all flow cytometry platforms, regardless of size or functionality. The assays are ... ER-ID® Green assay kit. ER-ID® Red assay kit (GFP-CERTIFIED®). GOLGI ID® Green assay kit. LYSO-ID® Green detection kit. LYSO-ID ... Optimized for reproducibility on microplate, flow cytometry, or fluorescent imaging platforms. *Widely published in peer- ... Compatible with common dyes and fluorescent markers (i.e., GFP) for multiplex analysis ...
Automated Washing of Multiplex Bead Based Assays for the Luminex MAGPIX® Reader System - Comparison of Luminex 100® and MAGPIX ... Both beads can be read using a flow cytometry based reader that interprets the bead type as well as quantitate the analyte. ... The multiplex cancer biomarker assay was found to be very repeatable in multiple assay runs carried out on separate days. ... Imaging & microscopy-focused Visual Abstracts explain the Augmented Microscopy workflow for a wide range of assays, with key ...
Cytokine multiplex assay. Flow-cytometry sorted Ly-6C- and Ly-6C+ CD4 TN cells from LNs of C57BL/6 Foxp3-GFP mice were ... Cell culture and in vitro polarization assays. Flow-cytometry sorted Ly-6C- and Ly-6C+ CD4 TN cells from LNs of C57BL/6 Foxp3- ... Imaging flow cytometry. LNs cells of C57BL/6 mice were harvested and fixed in 4% paraformaldehyde, immediately or after 30 min ... I) Cells were analyzed by imaging flow cytometry and NFAT1 nuclear localization assessed as in F. Data are representative of ...
... available from endpoint assays and greatly simplify assay protocols compared to using flow cytometry or high content imaging. ... The reagents are well tolerated by live cells which enables subsequent multiplexing of other assays such as extraction of RNA ... The real-time viability assay also provides a new tool to enable repeated dose toxicity experiments extending for weeks. Assay ... These new luciferase assay technologies to monitor cell viability and apoptosis in real-time from the same samples provide ...
... and IHC validated antibodies for imaging mass cytometry (IMC) ... CST offers flow validated antibodies for single cell mass ... The ability to analyze multiple markers in techniques such as flow cytometry and fluorescent multiplex immunohistochemistry ( ... Spectral overlap limits the number of fluorochromes that can be concurrently used in a cell or tissue fluorescent assay. In ... addition, tissue autofluorescence and weak signal intensity can make fluorescent IHC assays more challenging. Cycling amplified ...
High-throughput flow cytometry bead-based multiplex assay for identification of Rho GTPase inhibitors -- Functional analysis of ... Multiplex imaging of Rho family GTPase activities in living cells -- FRET-based imaging of rac and cdc42 activation during fc- ... Infectivity assays of human rhinovirus-A and -B serotypes / Wai-Ming Lee ... [et al.] -- Application of FCS in studies of ... Autofluorescent proteins for flow cytometry / Charles G. Bailey and John E. J. Rasko -- Fluorescent protein reporter systems ...
Image and distinguish cell cycle phases using Propidium Iodide (PI), BrdU/DAPI and EdU/DAPI using the Celigo cytometer from ... cell imaging cytometer allows users to conduct cell cycle analysis on adherent cells using assays typically developed for flow ... Results: Cell Cycle Assay using A375 Cells with Staurosporine-Treated Positive Control. ... It has the potential to be multiplexed with a marker for mitosis such as histone H3 phosphorylation. ...
Flow-FISH uses flow cytometry to perform FISH automatically using per-cell fluorescence measurements. Microfluidics-assisted ... Separate but compatible signal amplification systems enable the multiplex assay (up to two targets per assay). Signal ... Single-molecule RNA FISH assays can be performed in simplex or multiplex, and can be used as a follow-up experiment to ... or imaged simultaneously with a fluorescent antibody assay. The technology has potential applications in cancer diagnosis, ...
... optic Flow cytometric cell or particle counters, or coulter/impedance principle based cell counters Imaging assays, that ... Usual assays are simple or single target assays which is usually the default unless it is called multiplex. Multiplex assays ... involve image analysis manually or by software: Cytometry: When the size statistics of cells is assessed by an image processor ... DNase footprinting assay Filter binding assay Gel shift assay Bicinchoninic acid assay (BCA assay) Bradford protein assay Lowry ...
  • Mass cytometry facilitates high-dimensional, quantitative analysis of the effects of bioactive molecules on samples at single-cell resolution utilizing metal labeled antisera. (mdanderson.org)
  • Mass cytometry employs antibodies covalently labeled with non-radioactive isotopes of the lanthanide and noble metals as tags to perform massively multiparametric flow cytometry in a quantitative manner. (mdanderson.org)
  • This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. (abcam.com)
  • Mass cytometry offers advantages for multi-parameter analysis over flow and IHC applications, using metal-tagged antibodies (e.g. antibodies conjugated to rare metals using MaxPar ® conjugation kits). (cellsignal.com)
  • Specifically, mass cytometry or CyTOF ® uses time-of-flight mass spectrometry to phenotype cells and analyze cell functions, such as signaling. (cellsignal.com)
  • Imaging Mass Cytometry ™ combines CyTOF ® capabilities with imaging for spatial context. (cellsignal.com)
  • For single-cell mass cytometry, such as Helios ™ (from Fluidigm), consider selecting from CST's catalog of flow cytometry-validated antibodies. (cellsignal.com)
  • For Imaging Mass Cytometry ™ , the antibody you choose should be matched to your sample preparation method. (cellsignal.com)
  • Commonly Occurring Cell Subsets in High-Grade Serous Ovarian Tumors Identified by Single-Cell Mass Cytometry. (cellsignal.com)
  • Simultaneous Multiplexed Imaging of mRNA and Proteins with Subcellular Resolution in Breast Cancer Tissue Samples by Mass Cytometry. (cellsignal.com)
  • Mass Cytometry) and imagining (MIBI-Multiplexed Ion Beam Imaging) we are creating templates of ?normal? (stanford.edu)
  • Unlike other immunoassays, i.e. flow cytometry, ELISA and bead assays, which solely quantify the biomarker in a sample, a multiplex immunoassay quantifies the biomarker, identifies the location of the cell in the tissue and identifies the spatial relationship of the biomarkers with respect to each other. (labroots.com)
  • Recognition reactions of several analytes, such as immunoassays or hybridisation assays, take place on the surface of the beads, while a reporter fluorophore is used for the detection of the interaction between the recognition molecules and the analyte. (els.net)
  • Quickly and easily find protocols, webinars and guides across a wide range of topics and research areas, or learn about our latest products -- including recombinant antibodies, protein and miRNA assays, and custom services -- in our virtual exhibition hall. (labroots.com)
  • In addition, Enzo Life Sciences offers more than 600 antibodies that have been validated for flow cytometry. (enzolifesciences.com)
  • Cell Signaling Technology (CST) offers carrier-free antibodies to be used in highly multiplexed single-cell analysis and imaging. (cellsignal.com)
  • The ability to analyze multiple markers in techniques such as flow cytometry and fluorescent multiplex immunohistochemistry (IHC) requires the use of antibodies. (cellsignal.com)
  • The Opal method allows use of any number of unlabeled primary antibodies from the same species in multiplexed tissue assays, with no fear of cross-reactivity. (perkinelmer.com)
  • Berger SS, Lauritsen KT, Boas U, Lind P and Andresen LO (2017) Simultaneous detection of antibodies to five Actinobacillus pleuropneumoniae serovars using bead‐based multiplex analysis. (els.net)
  • During each assay cycle, the cells are stained with fluorescent- labeled antibodies, followed by image recording, and then fluorescence bleaching. (slideshare.net)
  • Cytotoxic T Cells can be cultured with activating antibodies such as anti-CD3 and tested for their ability to kill tumor targets in an IncuCyte based assay. (criver.com)
  • The panels' four dry, premixed antibody tubes use the company's Dry Unitized Reagent Assays Innovations technology, which eliminates the need to pipette antibodies. (aacc.org)
  • The alamarBlue and PrestoBlue assays are designed to be simple and convenient by providing the reagents in a proprietary stabilizing formulation with a "add, incubate, and read" protocol that is scalable from single wells to high-throughput screening (HTS). (thermofisher.com)
  • Our colorimetric viability assays the Vybrant MTT assay and CyQUANT XTT assay include all the reagents necessary to generate accurate and consistent colorimetric viability results. (thermofisher.com)
  • Multiplexing CellEvent and PrestoBlue reagents using high-throughput screening (HTS). (thermofisher.com)
  • In addition to a wide selection of gold standard labeling dyes such as DAPI, Hoechst, and JC-1, Enzo Life Sciences has translated its expertise in fluorescent probe chemistry and cellular analysis into our CELLestial® portfolio of unique probe-based assays and reagents to meet the emerging needs of the life sciences and drug discovery markets. (enzolifesciences.com)
  • Our preference is to begin working with VDC investigators early in the study design process so that we can provide expertise and experience in assay and sampling selection (both time points and tissue selection), perform QA/QC for sample processing (collecting, storing and shipping), and assure the timely procurement and validation of assay reagents. (cavd.org)
  • Intellicyt iQue® Screener PLUS - platform integrates a flow based instrument with enabling and easy to use software and reagents designed to address key challenges across the suspension-cell screening workflow. (sartorius.com)
  • If the assay involves exogenous reactants (the reagents), then their quantities are kept fixed (or in excess) so that the quantity and quality of the target are the only limiting factors. (wikipedia.org)
  • We have come a long way from the less sensitive complement based cytotoxic assays and ELISA to the newer and more sensitive multiplexed particle based flow cytometry (e.g. (blogspot.com)
  • Supernatants were collected after 4 days and assayed for IFNγ secretion by ELISA. (creativebiolabs.net)
  • Biochemical assays - protein analysis, cell viability readouts, antibody conjugation. (thebigchoice.com)
  • 1993). Multiplexed biochemical assays with biological chips. (freepatentsonline.com)
  • Some assays (e.g., biochemical assays) may be similar to chemical analysis and titration. (wikipedia.org)
  • Using a Luminex-based approach to multiplex in vivo assays, we determined that the domain of YAP that interacts with the TEAD/TEF family of transcription factors but not the WW domains or PDZ-binding motif, is essential for YAP-mediated tumor growth and metastasis. (pnas.org)
  • Luminex, see image). (blogspot.com)
  • Knowing that high throughput would be important for the gut dysbiosis test, scientists once again turned to Luminex as they designed the new 50-probe assay. (luminexcorp.com)
  • Complement binding DSA, as judged by C1q fixing on single bead assays, is associated with a worse allograft prognosis, with some evidence that C3d fixation may also be important (previous RFN post here ). (blogspot.com)
  • Usually expressed as the Mean Fluorescence Intensity (MFI) on the solid phase (single bead) assay. (blogspot.com)
  • Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. (antibodies-online.com)
  • We developed a quantitative immunofluorescence assay to measure stromal expression of CD3, CD8, and CD20 on one slide. (aacrjournals.org)
  • The lymphocyte percentage and CD3, CD8, and CD20 proportions were similar between flow cytometry and quantitative immunofluorescence on tonsil specimens. (aacrjournals.org)
  • 3. Incurred sample reanalysis (ISR) in cytometry The implementation of standardized cytometry-based biomarker assays in clinical trials remains a challenge due to the limited stability of clinical specimens and inter-instrument variations. (slideshare.net)
  • This includes validated biomarker assays, utilizing multiplex readout technologies such as MSD's MESO QuickPlex SQ120 for rapid highly-sensitive results and Akoya Biosciences ® Vectra ® Polaris™ Automated Quantitative Pathology Imaging System. (malvernpanalytical.com)
  • Imaging cytometers, such as the Hyperion ™ (from Fluidigm), are able to simultaneously image dozens of biomarkers in FFPE or frozen tissue samples to provide information on tissue architecture, protein co-localization, and cellular interactions. (cellsignal.com)
  • Opal ™ is a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image. (perkinelmer.com)
  • 1. HIGH-CONTENT CYTOMETRY _______________________________________________________________________________________________ High-Content Cytometry Volume 12 D Clinical Immunology Five Things to Consider when Developing Clinical Biomarkers eveloping cellular biomarkers is particularly challenging when it comes to biomarker validation, because flow cytometry has considerable limitations with respect to sample stability and multiplexing. (slideshare.net)
  • Contrary to flow cytometry, the cells are immobilized inside a microfluidic channel, thus allowing the stepwise quantitative analysis of unlimited numbers of biomarkers on the same cells with very little hands-on time [1, 2, 3, 4]. (slideshare.net)
  • Akoya Biosciences® Vectra® Polaris™ Automated Quantitative Pathology Imaging System to create high-quality fluorescent images of multiple biomarkers on a single section. (malvernpanalytical.com)
  • Concept's highly experienced bioanalytical team have decades of know-how in developing and validating novel chromatographic assays to detect and quantify small molecule biomarkers. (malvernpanalytical.com)
  • This allows us to create stunning high-quality fluorescent images of multiple biomarkers on a single section. (malvernpanalytical.com)
  • Our highly experienced immunologists are able to deliver a wide range of customizable multiplex analyses of protein biomarkers. (malvernpanalytical.com)
  • Lab testing for new cancer biomarkers - hormone, receptor and protein assays - improve the detection of cancer in patients that present with symptoms. (gii.co.jp)
  • There are currently no images for IgG (H+L) Antibody (NB120-7137). (novusbio.com)
  • There are currently no images for Human Kappa Light Chain Antibody (NBP1-73598). (novusbio.com)
  • IL-2-activated human PBMCs (effector cells) were incubated with activated human CD4+ T cells (target cells) in an effector-to-target cell ratio of 50:1 in the presence of serial dilutions of nivolumab or a positive control anti-MHC class I antibody for 3 hour at 37°C. A-C, data from three individual ADCC assays using cells from different donors are shown. (creativebiolabs.net)
  • April 27, 2009 - See Notice NOT-RM-09-011 Notice of Opportunity for Fast Track Entry of Assay Projects for High Throughput Screening into the NIH Roadmap Molecular Libraries Probe Production Centers Network. (nih.gov)
  • In this application note from PromoCell , explore the cytotoxic and cytostatic effects of drug treatment on a variety of primary human cell types and find out how these clinically-relevant effects can be monitored with high throughput-suitable cell-based assays that can closely reproduce in vivo physiological conditions. (selectscience.net)
  • Flow cytometry offers high multiplexing potential and a high‐throughput format. (els.net)
  • Armstrong B, Stewart M and Mazumder A (2000) Suspension arrays for high throughput multiplexed single nucleotide polymorphism genotyping. (els.net)
  • Highlights of Genedata Screener 17.0 include the embedding of experimental data in its biological context for advanced decision support and reporting, increased time savings through further automation of data capture and analysis, new biomolecule quantification and characterization methods for experiments using biolayer interferometry or high throughput flow cytometry, and extension of the unmatched technology coverage of the software by adding analyses for transcriptomic screening assays. (genedata.com)
  • Enhanced quality control and interactive visualization capabilities in high throughput flow cytometry-a critical technique for large molecule discovery-add further value to the automated analysis of large molecule campaigns with Genedata Screener. (genedata.com)
  • The software quantifies high throughput multiplexed gene expression data after compound treatment. (genedata.com)
  • easyCyte Flow Cytometers use novel microcapillary technology that enables a compact user-friendly format, which is powerful enough to run your most complex assays. (luminexcorp.com)
  • The CCVIMC provides both standardized, and a variety of research, assays-including an expanding repertoire of B cell, tissue imaging, and immune complex assays-to CAVD investigators for both human clinical trials and NHP studies. (cavd.org)
  • From simple to complex assays, Assay Catalog consistently captures assay biology and experimental conditions and links screening data to them. (genedata.com)
  • Opal Multiplex IHC Kits make multiplex methods accessible to researchers who work with standard immunohistochemistry (IHC). (perkinelmer.com)
  • Flow cytometry is a laboratory analytical technique that can rapidly measure multiple parameters of individual cells or particles as they pass through a beam of light, typically a laser. (trimarkpublications.com)
  • Unlike bulk cell separation techniques such as immuno-panning or magnetic column separation, flow cytometry-based cell sorting instruments measure, classify and then sort individual cells or particles serially at rates of several thousand cells per second or higher. (google.com)
  • Flow cytometry-based cell sorting has been successfully applied to hundreds of cell types, cell constituents and microorganisms, as well as many types of inorganic particles of comparable size. (google.com)
  • Blazer LL, Roman DL, Muxlow MR and Neubig RR (2010) Use of flow cytometric methods to quantify protein-protein interactions. (els.net)
  • We developed a highly multiplexed screen to quantify the co-expression of 351 surface molecules on millions of human B cells. (stanford.edu)
  • The release of soluble cytosolic enzymes, such as lactate dehydrogenase (LDH) or glucose 6-phosphate dehydrogenase release assay (G6PD) assays, into the supernatant can be easily quantified. (thermofisher.com)
  • It has long been used in cell counting and cell sorting but more recently, researchers have been using flow cytometry in a broad range of applications including: biomarker detection, cell signaling, pathway screening, protein engineering, and systems biology. (enzolifesciences.com)
  • Thus, these iQdots are potentially useful as optical probes for sensitive, multiplexed detection of surface markers on tumor cells. (pubmedcentralcanada.ca)
  • In recent years optical imaging has emerged as a sensitive detection method for diagnostic and therapeutic purposes. (pubmedcentralcanada.ca)
  • The characterisation and classification of beads in different groups, as well as the detection of the fluorescence emitted from the reporter molecules is accomplished by a flow cytometer, as the beads pass one by one in front of one or two laser beams. (els.net)
  • Flow cytometry is applied for the detection of various analytes. (els.net)
  • Binder SR (2006) Autoantibody detection using multiplex technologies. (els.net)
  • The huge potential of aptamer-nanoparticle-based detection of cancer cells and biomedical in - vivo imaging has been shown, with some examples. (smw.ch)
  • Aptamer-modified nanoparticles for medical applications are developed mostly for the detection of analytes, for biomedical in-vivo imaging and for in-vivo drug delivering. (smw.ch)
  • This is the precondition for provision of these new detection, imaging and therapy methods. (smw.ch)
  • Assays can be very diverse, but generally involve the following general steps: Sample processing and manipulation in order to selectively present the target in a discernible or measurable form to a discrimination/identification/detection system. (wikipedia.org)
  • The quantitative assay demonstrates improved reproducibility and similar specificity and sensitivity compared with traditional TIL classification. (aacrjournals.org)
  • The analysis is based on MACSPlex Capture Beads, which display defined fluorescence properties and can be identified using standard flow cytometry techniques. (miltenyibiotec.com)
  • As a result, easyCyte™ Flow Cytometers are uniquely amenable to on-demand use in the laboratory environment and have helped many scientists achieve insightful cellular analysis since 1998. (luminexcorp.com)
  • This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. (antibodies-online.com)
  • Visit the Sartorius booth #B5-6, at Elrig Flow Cytometry & Imaging 2018, to learn the latest on the IncuCyte® S3 Live-Cell Analysis System and the Intellicyt® iQue Screener PLUS. (essenbioscience.com)
  • Downstream assays for liquid analysis of redox-active molecules are shown, and target cells are plasma-treated. (jove.com)
  • Current instrumentation allows simultaneous quantitative analysis of up to 12 parameters, each of which is assayed at the individual cell level, facilitating high content analysis of mixed cell populations and rare cell types. (mdanderson.org)
  • The Core facility provides researchers with technical expertise in assay development, data acquisition and various data analysis techniques. (mdanderson.org)
  • Enzo Life Sciences offers a variety of flow cytometry-based assays for cell viability, proliferation and death analysis, available in a wide range of formats to meet individual research needs. (enzolifesciences.com)
  • The Celigo cell imaging cytometer allows users to conduct cell cycle analysis on adherent cells using assays typically developed for flow cytometry. (nexcelom.com)
  • The Intellicyt iQue3 from Sartorius is designed to be a comprehensive solution for the multiplexed analysis of cells and beads. (selectscience.net)
  • This advanced flow cytometry platform promises rapid setup, analysis, and sample acquisition, plus a newly integrated Enhanced Rinse Station. (selectscience.net)
  • Download this white paper to find out how the NoviSight™ 3D software from Olympus can perform quantitative analysis of PDOs, evaluating their morphology and pharmacological responses, using images captured by Olympus' FLUOVIEW ® FV3000 confocal laser scanning microscope. (selectscience.net)
  • Furthermore, since the emission wavelength is readily tuned by controlling the size of the Qdots, they can be synthesized to emit different colors, allowing multiplex imaging which is increasingly important in the analysis of complex biological systems ( 2 ). (pubmedcentralcanada.ca)
  • This approach provides information not available from alternative techniques like flow cytometry or analysis of single markers in serial sections. (perkinelmer.com)
  • The purpose of this TriMark Publications report is to provide a detailed analysis of the global flow cytometry market, including size, growth, technology platforms, applications, new instrumentation, industry trends and the internal structure of the sector. (trimarkpublications.com)
  • Principle of the analysis of beads by flow cytometry. (els.net)
  • NanoString service for multiplex analysis of up to 800 RNA, DNA or protein targets. (malvernpanalytical.com)
  • Up to nine markers (8 + DAPI) (9-plex) can be visualized at 10-40x, allowing for image analysis, quantification and phenotyping of samples. (malvernpanalytical.com)
  • The team will position the portfolio peer to peer via technical talks, scientific conferences and technical poster presentations highlighting multiplex protein analysis assays in 384 well HTS format and 96 well screening formats along with multiplex assays. (evolutionexec.com)
  • New imaging modalities and digital analysis of x-ray mammograms are looking to improve the accuracy of breast cancer screening. (gii.co.jp)
  • This volume highlights recent developments in flow cytometry, affinity assays, imaging, mass spectrometry, microfluidics and other technologies that enable analysis of proteins at the single cell level. (springer.com)
  • The data generated from these assays are provided to the Vaccine Immunology Statistical Center (VISC) for statistical analysis and reported directly to the Vaccine Discovery Consortia (VDC) for the evaluation of the impact of data on the results of the trial. (cavd.org)
  • Genedata, the leading provider of enterprise software solutions for biopharmaceutical R&D, today announced the release of Genedata Screener ® 17.0, the latest version of its enterprise software for the automated analysis, visualization, and management of screening data from all in-vitro screening assay technologies. (genedata.com)
  • The new Assay Catalog in Genedata Screener 17.0 allows the definition of terminology for assays, annotations, and result types, registers assays, and connects experimental data to them, thus enabling automated and AI-supported downstream analysis. (genedata.com)
  • An important infrastructure complement to the data analysis and management modules in Genedata Screener, Assay Catalog places rich experimental data into biological context, straight out of the box. (genedata.com)
  • A fully automated workflow-from image capture, through image analysis and data analysis, to reporting to a corporate data warehouse-can be set up by combining Genedata Screener 17.0 with the recently released Genedata Imagence ® 2.0, both parts of the Genedata Biopharma Platform. (genedata.com)
  • For assay of currency coins this literally meant analysis of the purity of the gold or silver (or whatever the precious component) that represented the true value of the coin. (wikipedia.org)
  • An assay (analysis) is never an isolated process, as it must be accompanied with pre- and post-analytic procedures. (wikipedia.org)
  • What is Multiplex and what is the role of multiplex in Digital Pathology? (labroots.com)
  • While similar to traditional fluorescence based flow cytometry the use of metal labels removes the need for compensation and alleviates the limitations on the detectible number of parameters. (mdanderson.org)
  • The global flow cytometry market remains one of the fastest-growing segments of life sciences and clinical diagnostics markets. (trimarkpublications.com)
  • In the current life sciences research, pharmaceutical drug discovery and development, and clinical diagnostics markets, flow cytometry offers some of the brightest promise for growth and innovation. (trimarkpublications.com)
  • 2. Low sample consumption maximizes the information you get out of each clinical sample During conventional flow cytometry, the samples are lost with the flowing solution. (slideshare.net)
  • The Comprehensive Cellular Vaccine Immune Monitoring Consortium (CCVIMC) is a Central Service Facility (CSF) within the CAVD providing a variety of cellular assays in support of pre-clinical and clinical HIV vaccine trials. (cavd.org)
  • 4. Develop and optimize exploratory cellular assays and technologies for monitoring cellular immune responses in clinical and pre-clinical trials. (cavd.org)
  • A standardized IFN-g ELISpot assay is also available from our core of clinical and NHP labs. (cavd.org)
  • Clinical Virus Inhibition Assay for measuring CD8-mediated virus replication inhibition. (cavd.org)
  • The CCVIMC also provides CAVD investigators with a variety of clinical and NHP PBMC specimens, and some NHP tissue specimens to support assay development initiatives. (cavd.org)
  • Standardized assays as well as research assays have been provided for both clinical and pre-clinical (NHP) vaccine trials within the CAVD. (cavd.org)
  • Beckman Coulter has received Food and Drug Administration (FDA) clearance for both the Early Sepsis Indicator and the ClearLLab 10C system for clinical flow cytometry labs. (aacc.org)
  • IncuCyte based tumor killing assay enables quantification of target number that can be multiplexed with target specific apoptotic readout. (criver.com)
  • The new release supports biolayer interferometry (BLI) quantification assays in large molecule workflows, computing sample concentrations from this technique. (genedata.com)
  • From simple organelle specific dyes for imaging cell structure and determining cell viability, to more complex dyes and reporter assays for monitoring cell signaling, death pathways, and toxicity, every product is developed and reliably manufactured to provide sensitivity, specificity, and convenience. (enzolifesciences.com)
  • All of these iQdots retain the photoluminescence properties of the unconjugated Qdot 800 as well as the antigen binding specificity as demonstrated by flow cytometry. (pubmedcentralcanada.ca)
  • Multiplexed cellular readouts - Flow cytometry, fluorescence microscopy including live imaging of tissue sections. (thebigchoice.com)
  • Quantum dots (Qdots), nanometer scale semiconductor materials, represent an important class of fluorescent probe for biomolecular and cellular imaging ( 1 ). (pubmedcentralcanada.ca)
  • Beyond providing exploratory, qualified, standardized and GCLP-compliant cellular assays, the CCVIMC consults with the Vaccine Discovery Consortia (VDCs) on both pre-trial design and as well as post-trial/assay assessments. (cavd.org)
  • These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. (stanford.edu)
  • These assays retrieve content-rich biological information about plasma effects from a single plate. (jove.com)
  • However, assays typically involve biological material or phenomena that are intrinsically more complex in composition or behavior, or both. (wikipedia.org)
  • PD-1 expression on activated CD4+ T cells in each of the ADCC assays is shown in the right (solid line for PD-1, gray line for isotype control). (creativebiolabs.net)
  • MACSPlex Cytokine Assays are designed for determining concentrations of up to 7 soluble analytes in a single sample. (miltenyibiotec.com)
  • To better model how IO drugs interact with a patient's cancer cells and immune system, we have developed an in vitro assay based on the culture of human tumour samples. (thebigchoice.com)
  • Conventional flow cytometry requires fresh samples for biomarker development, and the cryopreservation of immune cells leads to significant changes in biomarker signatures. (slideshare.net)
  • In ChipCytometry, the original samples are stored on small microfluidic chips, where they can be assayed over and over again (Fig. 2). (slideshare.net)
  • These are now available in multiplexed tests that make better use of tissue samples. (gii.co.jp)
  • 6. Supply the CAVD community with quality-controlled, well characterized PBMC samples for use in the assay development and standardization. (cavd.org)
  • At the end of the assay the tissue samples are visualized under a fluorescence microscope. (wikipedia.org)
  • Microplate assays provide information on entire cell populations rather than tracking the behavior of individual cells. (thermofisher.com)
  • We offer microplate assays for whole cells and assays performed on disrupted cells or cell lysates. (thermofisher.com)
  • To complete or enhance your BioTek imager, microplate reader, liquid handling or robotics system, we offer a wide range of accessories, from tubing and bottles to imaging filter cubes. (biotek.com)
  • These simple fluidics enable a self-aligning flow cell that can be removed for cleaning by the user. (luminexcorp.com)
  • The flow cell is self-analyzing, and can be easily removed for cleaning-so you can say goodbye to having your acquisition delayed by a clogged flow cell. (luminexcorp.com)
  • DRAQ7 far-red live-cell impermeant DNA dye differentially stains both the nucleus and cytoplasm in dead, permeabilized or fixed mammalian cells, making it ideal for a HCS translocation (or Redistribution™ / Transfluor™) assay. (immunochemistry.com)
  • Because DRAQ7 is a far-red emitting DNA dye, this can be easily multiplexed with other parameters, like cell surface phenotype. (immunochemistry.com)
  • Multiplex for cell death with FLICA and DRAQ7. (immunochemistry.com)
  • Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). (stanford.edu)
  • Specifically, melanoma cells are analyzed in an efficient sequence of different consecutive assays but using the same cells: measurement of metabolic activity, total cell area, and surface marker expression of calreticulin, a molecule important for the immunogenic cell death of cancer cells. (jove.com)
  • To analyze the immune cell makeup, the predominant tool has been flow cytometry, he continues. (drugdiscoverynews.com)
  • Spectral overlap limits the number of fluorochromes that can be concurrently used in a cell or tissue fluorescent assay. (cellsignal.com)
  • Flow cytometry-based cell sorting was first introduced to the research community more than 20 years ago. (google.com)
  • This rapid "one-by-one" processing of single cells has made flow cytometry a unique and valuable tool for extracting highly pure sub-populations of cells from otherwise heterogeneous cell suspensions. (google.com)
  • B cell phenotyping is a standardized assay for NHP trial. (cavd.org)
  • This assay can be tailored to the anticipated mechanism of action by selecting from a panel of validated target cell lines. (criver.com)
  • in vitro 3D assays incorporate live cell imaging to predict in vivo responses to matched PDX material. (criver.com)
  • They will develop and execute a strategy to promote and implement the Company's multiplex assays at key pharma accounts and Key Opinion Leader laboratories. (evolutionexec.com)
  • Time-lapse images of cultured cancer and immune cells in 96-well microplates were automatically collected using an IncuCyte S3 live analyzer (Essen Bioscience). (sartorius.com)
  • Buranda T, Wu Y and Sklar LA (2009) Subsecond analyses of G‐protein coupled‐receptor ternary complex dynamics by rapid mix flow cytometry. (els.net)
  • Immunologic changes were monitored using flow cytometry and serum analyses. (diabetesjournals.org)
  • They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. (stanford.edu)
  • Owing to their multiple talents, aptamers combined with nanoparticles are nanosystems well qualified for the development of new biomedical devices for analytical, imaging, drug delivery and many other medical applications. (smw.ch)
  • Aptamer modified magnetic nanoparticles offer the possibility of magnetic resonance imaging as well as application of magnetic fluid hypothermia therapy [64]. (smw.ch)
  • Additional assay development efforts include multiplexed confocal imaging to assess the anatomic and structural features of germinal centers in response to vaccine strategies or SIV/SHIV infections. (cavd.org)
  • Schematic illustration of (a) an immunoassay and (b) a hybridisation assay on the bead's surface. (els.net)
  • Bellisario R, Colinas RJ and Pass KA (2000) Simultaneous measurement of thyroxine and thyrotropin from newborn dried blood‐spot specimens using a multiplexed fluorescent microsphere immunoassay. (els.net)
  • This driven scientific professional will lead a global team who evaluate and test laboratory equipment such as flow cytometers, high content imagers and offer automation support. (evolutionexec.com)
  • Using new segmentation algorithms, the boundaries of individual cells (typically 300-1000 per image) were identified in each image of the sequence. (sartorius.com)
  • The three parts of a flow cytometer: fluidics, optics and electronics. (els.net)
  • 10. The method of claim 1, wherein the one or more thioaptamers are attached to beads and the step of selecting one or more beads to which one or more proteins has bound is defined further as being fluorescent sorting using a flow-cytometer. (freepatentsonline.com)
  • We validated this assay by comparison with flow cytometry on tonsil specimens and assessed predictive value in breast cancer on a neoadjuvant cohort ( n = 95). (aacrjournals.org)
  • Metabolic activity is commonly used as a viability indicator, but for some applications it can be important to assess viability independent of metabolic state, and appropriate assays are listed below. (thermofisher.com)
  • The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. (stanford.edu)
  • The NIH Molecular Libraries Roadmap Initiative wishes to encourage HTS assay applications from investigators who have the interest and capability to work with the Molecular Libraries Probe Production Centers Network (MLPCN) for chemical probe development. (nih.gov)
  • BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek's instrumentation and software. (biotek.com)
  • Specifically, use of a unique in vitro assay system, the tissue slice culture, will be central to the role. (thebigchoice.com)
  • Figure 1: PBMC - mediated tumor killing in 2D format, and example image, demonstrating killing over time with Pembrolizumab/Ipilimumab with or without depletion of CD8+ cytotoxic T cells. (criver.com)
  • Separate but compatible signal amplification systems enable the multiplex assay (up to two targets per assay). (wikipedia.org)