High molecular weight mucoproteins that protect the surface of EPITHELIAL CELLS by providing a barrier to particulate matter and microorganisms. Membrane-anchored mucins may have additional roles concerned with protein interactions at the cell surface.
Mucins that are found on the surface of the gastric epithelium. They play a role in protecting the epithelial layer from mechanical and chemical damage.
A gel-forming mucin that is predominantly expressed by submucosal glands of airway tissues and the SUBLINGUAL GLAND. It is one of the principal components of high molecular weight salivary mucin.
Carbohydrate antigen elevated in patients with tumors of the breast, ovary, lung, and prostate as well as other disorders. The mucin is expressed normally by most glandular epithelia but shows particularly increased expression in the breast at lactation and in malignancy. It is thus an established serum marker for breast cancer.
A transmembrane mucin that is found in a broad variety of epithelial tissue. Mucin-4 may play a role in regulating cellular adhesion and in cell surface signaling from the ERBB-2 RECEPTOR PROTEIN-TYROSINE KINASE. Mucin-4 is a heterodimer of alpha and beta chains. The alpha and beta chains result from the proteolytic cleavage of a precursor protein.
A membrane-bound mucin subtype that is primarily found in INTESTINAL MUCOSA. Two closely-related subtypes of this protein have been identified in humans.
A gel-forming mucin that is predominantly associated with the gastric epithelium.
A glandular epithelial cell or a unicellular gland. Goblet cells secrete MUCUS. They are scattered in the epithelial linings of many organs, especially the SMALL INTESTINE and the RESPIRATORY TRACT.
The viscous secretion of mucous membranes. It contains mucin, white blood cells, water, inorganic salts, and exfoliated cells.
A subcategory of mucins that contain SIALIC ACID.
The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.
Carbohydrate antigens expressed by malignant tissue. They are useful as tumor markers and are measured in the serum by means of a radioimmunoassay employing monoclonal antibodies.
Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.
The cartilaginous and membranous tube descending from the larynx and branching into the right and left main bronchi.
A histochemical technique for staining carbohydrates. It is based on PERIODIC ACID oxidation of a substance containing adjacent hydroxyl groups. The resulting aldehydes react with Schiff reagent to form a colored product.
The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.
Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.
The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.
The mucous membrane that covers the posterior surface of the eyelids and the anterior pericorneal surface of the eyeball.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
A biomineral consisting of layers of crystalized ARAGONITE, separated by interlamellar layers of organic matrix containing beta-CHITIN, and surrounded by an intercrystalline organic membrane of silk fibroin. Nacre is the iridescent substance better known as mother-of-pearl that is secreted by MOLLUSCS.
The application of LUBRICANTS to diminish FRICTION between two surfaces.
A plant genus of the family Orobanchaceae that is parasitic on the roots of other plants. Members contain the flavones, APIGENIN and LUTEOLIN.
A genus of pearl oysters in the family Pteriidae, class BIVALVIA. Both cultured and natural pearls are obtained from species in the genus. They are distinct from the distantly related, edible true oysters of the family OSTREIDAE.
The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.
A gel-forming mucin found predominantly in SMALL INTESTINE and variety of mucous membrane-containing organs. It provides a protective, lubricating barrier against particles and infectious agents.
Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.
A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.
The procedures involved in combining separately developed modules, components, or subsystems so that they work together as a complete system. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Tumors or cancer of the PANCREAS. Depending on the types of ISLET CELLS present in the tumors, various hormones can be secreted: GLUCAGON from PANCREATIC ALPHA CELLS; INSULIN from PANCREATIC BETA CELLS; and SOMATOSTATIN from the SOMATOSTATIN-SECRETING CELLS. Most are malignant except the insulin-producing tumors (INSULINOMA).
Carcinoma that arises from the PANCREATIC DUCTS. It accounts for the majority of cancers derived from the PANCREAS.
A lesion with cytological characteristics associated with invasive carcinoma but the tumor cells are confined to the epithelium of origin, without invasion of the basement membrane.
Non-optimal interval of time between onset of symptoms, identification, and initiation of treatment.
Pathological processes that tend eventually to become malignant. (From Dorland, 27th ed)
A segment of the LOWER GASTROINTESTINAL TRACT that includes the CECUM; the COLON; and the RECTUM.
The portion of the GASTROINTESTINAL TRACT between the PYLORUS of the STOMACH and the ILEOCECAL VALVE of the LARGE INTESTINE. It is divisible into three portions: the DUODENUM, the JEJUNUM, and the ILEUM.
A strain of mice widely studied as a model for cystic fibrosis. These mice are generated from embryonic stem cells in which the CFTR (cystic fibrosis transmembrane conductance regulator) gene is inactivated by gene targeting. As a result, all mice have one copy of this altered gene in all their tissues. Mice homozygous for the disrupted gene exhibit many features common to young cystic fibrosis patients, including failure to thrive, meconium ileus, and alteration of mucous and serous glands.
An autosomal recessive genetic disease of the EXOCRINE GLANDS. It is caused by mutations in the gene encoding the CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR expressed in several organs including the LUNG, the PANCREAS, the BILIARY SYSTEM, and the SWEAT GLANDS. Cystic fibrosis is characterized by epithelial secretory dysfunction associated with ductal obstruction resulting in AIRWAY OBSTRUCTION; chronic RESPIRATORY INFECTIONS; PANCREATIC INSUFFICIENCY; maldigestion; salt depletion; and HEAT PROSTRATION.
A chloride channel that regulates secretion in many exocrine tissues. Abnormalities in the CFTR gene have been shown to cause cystic fibrosis. (Hum Genet 1994;93(4):364-8)
Any pathological condition where fibrous connective tissue invades any organ, usually as a consequence of inflammation or other injury.
Infections with bacteria of the genus PSEUDOMONAS.
A natural high-viscosity mucopolysaccharide with alternating beta (1-3) glucuronide and beta (1-4) glucosaminidic bonds. It is found in the UMBILICAL CORD, in VITREOUS BODY and in SYNOVIAL FLUID. A high urinary level is found in PROGERIA.
Coloring matter from the insect Coccus cacti L. It is used in foods, pharmaceuticals, toiletries, etc., as a dye, and also has use as a microscopic stain and biological marker.
The inner membrane of a joint capsule surrounding a freely movable joint. It is loosely attached to the external fibrous capsule and secretes SYNOVIAL FLUID.
A protective layer of firm, flexible cartilage over the articulating ends of bones. It provides a smooth surface for joint movement, protecting the ends of long bones from wear at points of contact.
Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.
The clear, viscous fluid secreted by the SALIVARY GLANDS and mucous glands of the mouth. It contains MUCINS, water, organic salts, and ptylin.
The oval-shaped oral cavity located at the apex of the digestive tract and consisting of two parts: the vestibule and the oral cavity proper.
One of a set of bone-like structures in the mouth used for biting and chewing.
A solution used for irrigating the mouth in xerostomia and as a substitute for saliva.

Complete sequence of the human mucin MUC4: a putative cell membrane-associated mucin. (1/2610)

The MUC4 gene, which encodes a human epithelial mucin, is expressed in various epithelial tissues, just as well in adult as in poorly differentiated cells in the embryo and fetus. Its N-terminus and central sequences have previously been reported as comprising a 27-residue peptide signal, followed by a large domain varying in length from 3285 to 7285 amino acid residues. The present study establishes the whole coding sequence of MUC4 in which the C-terminus is 1156 amino acid residues long and shares a high degree of similarity with the rat sialomucin complex (SMC). SMC is a heterodimeric glycoprotein complex composed of mucin (ascites sialoglycoprotein 1, ASGP-1) and transmembrane (ASGP-2) subunits. The same organization is found in MUC4, where the presence of a GlyAspProHis proteolytic site may cleave the large precursor into two subunits, MUC4alpha and MUC4beta. Like ASGP-2, which binds the receptor tyrosine kinase p185(neu), MUC4beta possesses two epidermal growth factor-like domains, a transmembrane sequence and a potential phosphorylated site. MUC4, the human homologue of rat SMC, may be a heterodimeric bifunctional cell-surface glycoprotein of 2.12 micrometers. These results confer a new biological role for MUC4 as a ligand for ErbB2 in cell signalling.  (+info)

Role of retinoid receptors in the regulation of mucin gene expression by retinoic acid in human tracheobronchial epithelial cells. (2/2610)

To investigate which retinoid receptors are critical in the regulation by all-trans-retinoic acid (RA) of the mucin genes MUC2, MUC5AC and MUC5B in cultured normal human tracheobronchial epithelial (NHTBE) cells, we used pan-RAR-, pan-RXR- and RAR- isotype (alpha, beta and gamma)-selective agonists and RARalpha- and RARgamma-selective antagonists (RAR is RA receptor and RXR is retinoid X receptor). RAR-, RARalpha- and RARgamma-selective agonists strongly induced mucin mRNAs in a dose-dependent manner, while the RARbeta-selective retinoid only weakly induced mucin gene expression at very high concentrations (1 microM). The pan-RXR-selective agonist by itself did not induce mucin gene expression, but acted synergistically with suboptimal concentrations of the pan-RAR agonist. A retinoid with selective anti-activator-protein-1 activity only marginally induced mucin gene expression. The RARalpha antagonist strongly inhibited mucin gene induction and mucous cell differentiation caused by RA and by the RARalpha- and RARgamma-selective retinoids. In contrast, the RARgamma antagonist only weakly inhibited RARalpha-selective-retinoid-induced mucin gene expression, but completely blocked mucin gene expression induced by the RARgamma-selective retinoid. Our studies indicate that RARalpha is the major retinoid receptor subtype mediating RA-dependent mucin gene expression and mucous cell differentiation, but that the RARgamma isotype can also induce mucin genes. Furthermore these studies suggest that RARbeta is probably not (directly) involved in RA-induced mucin gene expression.  (+info)

Physical characterization of a low-charge glycoform of the MUC5B mucin comprising the gel-phase of an asthmatic respiratory mucous plug. (3/2610)

We have previously noted that sequential extraction of an asthmatic mucous exudate with 6 M guanidinium chloride yielded a fraction of the mucins that were most resistant to solubilization and of high Mr [Sheehan, Richardson, Fung, Howard and Thornton (1995) Am. J. Respir. Cell Mol. Biol. 13, 748-756]. Here we show that this mucin fraction is dominated (at least 96% of the total) by the low-charge glycoform of the MUC5B gene product. Seen in the electron microscope the mucins appeared mainly as compact 'island' structures composed of linear threads often emanating from globular 'nodes' rather than the discrete linear threads more typical of mucins that we have previously described. The effect of reducing agents was as expected for other gel-forming mucins, i.e. reduced subunits or monomers of Mr 3x10(6)) were produced within 15 min of treatment. Kinetic experiments on the cleavage of the intact mucins with the proteinase trypsin indicated two clear regimes of fragmentation. An initial rapid cleavage generated mucins ranging from Mr=4x10(6) to 30x10(6) that in the electron microscope appeared as polydisperse threads (500-3000 nm in length), similar to normal and other respiratory mucins that we have previously characterized. A subsequent slower fragmentation over many hours yielded a major fragment of Mr 3x10(6) and length 200-600 nm, very similar in size and Mr to the subunits obtained by reduction. The results suggest that the MUC5B mucin is assembled, first into polydisperse linear threads, which are then linked together via a protein-mediated process. This might involve part of the mucin polypeptide or an as yet unidentified protein(s). The high proteinase susceptibility of the linkage suggests that it might be a point of control for mucin size and thus mucus rheology.  (+info)

Salivary mucin MG1 is comprised almost entirely of different glycosylated forms of the MUC5B gene product. (4/2610)

The MG1 population of mucins was isolated from human whole salivas by gel chromatography followed by isopycnic density gradient centrifugation. The reduced and alkylated MG1 mucins, separated by anion exchange chromatography, were of similar size (radius of gyration 55-64 nm) and molecular weight (2.5-2.9 x 10(6) Da). Two differently-charged populations of MG1 subunits were observed which showed different reactivity with monoclonal antibodies to glycan epitopes. Monosaccharide and amino acid compositional analyses indicated that the MG1 subunits had similar glycan structures on the same polypeptide. An antiserum recognizing the MUC5B mucin was reactive across the entire distribution, whereas antisera raised against the MUC2 and MUC5AC mucins showed no reactivity. Western blots of agarose gel electrophoresis of fractions across the anion exchange distribution indicated that the polypeptide underlying the mucins was the product of the MUC5B gene. Amino acid analysis and peptide mapping performed on the fragments produced by trypsin digestion of the two MG1 populations yielded data similar to that obtained for MUC5B mucin subunits prepared from respiratory mucus (Thornton et al., 1997) and confirmed that the MUC5B gene product was the predominant mucin polypeptide present. Isolation of the MG1 mucins from the secretions of the individual salivary glands (palatal, sublingual, and submandibular) indicate that the palatal gland is the source of the highly charged population of the MUC5B mucin.  (+info)

The sialylation of bronchial mucins secreted by patients suffering from cystic fibrosis or from chronic bronchitis is related to the severity of airway infection. (5/2610)

Bronchial mucins were purified from the sputum of 14 patients suffering from cystic fibrosis and 24 patients suffering from chronic bronchitis, using two CsBr density-gradient centrifugations. The presence of DNA in each secretion was used as an index to estimate the severity of infection and allowed to subdivide the mucins into four groups corresponding to infected or noninfected patients with cystic fibrosis, and to infected or noninfected patients with chronic bronchitis. All infected patients suffering from cystic fibrosis were colonized by Pseudomonas aeruginosa. As already observed, the mucins from the patients with cystic fibrosis had a higher sulfate content than the mucins from the patients with chronic bronchitis. However, there was a striking increase in the sialic acid content of the mucins secreted by severely infected patients as compared to noninfected patients. Thirty-six bronchial mucins out of 38 contained the sialyl-Lewis x epitope which was even expressed by subjects phenotyped as Lewis negative, indicating that at least one alpha1,3 fucosyltransferase different from the Lewis enzyme was involved in the biosynthesis of this epitope. Finally, the sialyl-Lewis x determinant was also overexpressed in the mucins from severely infected patients. Altogether these differences in the glycosylation process of mucins from infected and noninfected patients suggest that bacterial infection influences the expression of sialyltransferases and alpha1,3 fucosyltransferases in the human bronchial mucosa.  (+info)

Sulphation and secretion of the predominant secretory human colonic mucin MUC2 in ulcerative colitis. (6/2610)

BACKGROUND: Decreased synthesis of the predominant secretory human colonic mucin (MUC2) occurs during active ulcerative colitis. AIMS: To study possible alterations in mucin sulphation and mucin secretion, which could be the cause of decreased mucosal protection in ulcerative colitis. METHODS: Colonic biopsy specimens from patients with active ulcerative colitis, ulcerative colitis in remission, and controls were metabolically labelled with [35S]-amino acids or [35S]-sulphate, chase incubated and analysed by SDS-PAGE, followed by quantitation of mature [35S]-labelled MUC2. For quantitation of total MUC2, which includes non-radiolabelled and radiolabelled MUC2, dot blotting was performed, using a MUC2 monoclonal antibody. RESULTS: Between patient groups, no significant differences were found in [35S]-sulphate content of secreted MUC2 or in the secreted percentage of either [35S]-amino acid labelled MUC2 or total MUC2. During active ulcerative colitis, secretion of [35S]-sulphate labelled MUC2 was significantly increased twofold, whereas [35S]-sulphate incorporation into MUC2 was significantly reduced to half. CONCLUSIONS: During active ulcerative colitis, less MUC2 is secreted, because MUC2 synthesis is decreased while the secreted percentage of MUC2 is unaltered. Furthermore, sulphate content of secreted MUC2 is unaltered by a specific compensatory mechanism, because sulphated MUC2 is preferentially secreted while sulphate incorporation into MUC2 is reduced.  (+info)

Mucin expression and function in the female reproductive tract. (7/2610)

Reproductive tract epithelia are characterized by the presence of a thick, apical glycocalyx. This glycoprotein coat is drastically reduced in the uterus of many species during the time of embryo implantation. Recent studies indicate that mucin glycoproteins constitute a large proportion of the apical glycocalyx. One of these mucins, Muc-1, has particularly important functions at the luminal surface of the uterus and other female reproductive tract tissues. Muc-1 appears to play a dominant role in maintaining a functionally non-receptive uterine surface with regard to blastocyst attachment. Conversion to a receptive uterine state is brought about by the concerted actions of ovarian steroid hormones that in several species also strongly modulate Muc-1 protein and mRNA expression. Muc-1 also appears to serve a general function in protecting reproductive tract mucosa since Muc-1 null mice are particularly prone to bacterial infection. Collectively, these studies indicate that mucins, including Muc-1, play important barrier roles in reproductive processes and protection from bacterial pathogenesis in the female reproductive tract.  (+info)

pH-dependent conformational change of gastric mucin leads to sol-gel transition. (8/2610)

We present dynamic light scattering (DLS) and hydrophobic dye-binding data in an effort to elucidate a molecular mechanism for the ability of gastric mucin to form a gel at low pH, which is crucial to the barrier function of gastric mucus. DLS measurements of dilute mucin solutions were not indicative of intermolecular association, yet there was a steady fall in the measured diffusion coefficient with decreasing pH, suggesting an apparent increase in size. Taken together with the observed rise in depolarized scattering ratio with decreasing pH, these results suggest that gastric mucin undergoes a conformational change from a random coil at pH >/= 4 to an anisotropic, extended conformation at pH < 4. The increased binding of mucin to hydrophobic fluorescent with decreasing pH indicates that the change to an extended conformation is accompanied by exposure of hydrophobic binding sites. In concentrated mucin solutions, the structure factor S(q, t) derived from DLS measurements changed from a stretched exponential decay at pH 7 to a power-law decay at pH 2, which is characteristic of a sol-gel transition. We propose that the conformational change facilitates cross-links among mucin macromolecules through hydrophobic interactions at low pH, which in turn leads to a sol-gel transition when the mucin solution is sufficiently concentrated.  (+info)

Mucins are large glycoproteins that cover epithelial surfaces of the body. All mucins contain at least one PTS domain, a region rich in proline, threonine and serine. Mucins are also characterized by von Willebrand D (VWD) domains or SEA domains. We have developed computational methods to identify mucin genes and proteins based on these properties of the proteins. Using such methods we are able to characterize different organisms where genome sequence is available with respect to their mucin repertoire. We have here made a comprehensive analysis of potential mucins encoded by the chicken (Gallus gallus) genome. Three transmembrane mucins (Muc4, Muc13, and Muc16) and four gel-forming mucins (Muc6, Muc2, Muc5ac, and Muc5b) were identified. The gel-forming mucins are encoded within a locus similar to the corresponding human mucins. However, the chicken has an additional gene inserted between Muc2 and Muc5ac that encodes the the α-subunit of ovomucin, a protein similar to Muc2, but it is lacking a PTS
There is intense interest in how bacteria interact with mucin glycoproteins in order to colonise mucosal surfaces. In this study, we have assessed the feasibility of using recombinant mucin glycoproteins to study the interaction of the gastric pathogen Helicobacter pylori with MUC5AC, a mucin which the organism exhibits a distinct tropism for. Stable clonal populations of cells expressing a construct encoding for a truncated version of MUC5AC containing N- and C-termini interspersed with two native tandem repeat sequences (N + 2TR + C) were generated. Binding of H. pylori to protein immunoprecipitated from cell lysates and supernatants was assessed. High molecular weight mucin could be detected in both cell lysates and supernatants of transfected cells. Recombinant protein formed high molecular weight oligomers, was both N and O glycosylated, underwent cleavage similar to native MUC5AC and was secreted from the cell. H. pylori bound better to secreted mucin than intracellular mucin suggesting that
McGuckin M.A., Wright G. and Ward B.G. (1991) Expression of a polymorphic epithelial mucin antigen defined by the monoclonal antibody BC2 in ovarian carcinoma: Use of the BC2 antibody for the detection of micrometastases. American Journal of Clinical Pathology, 96 1: 46-52. ...
0005] The glycocalyx of the corneal epithelium contains the transmembrane mucin (glycosylated glycoproteins present in the glycocalyx), MUC1, MUC4, and MUC16. Mucins are high molecular weight Glycoproteins that provide a protective layer on corneal epithelial surfaces and are involved in cell-cell interactions, signaling, and metastasis. MUCIN 1, a cell surface associated (MUC 1) or polymorphic epithelial mucin (PEM) is a mucin, the protein part of which is encoded by the MUC1 gene in humans. Mucin proteins penetrate the membranes of epithelial cells, on the inner surface of the intestine and other organs. Mucin protects the body from infection by binding to pathogens. These membrane mucins interact with soluble, secreted, gel-forming mucin produced by the goblet cells (MUC5AC) and also with others like MUC2. The lacrimal gland also secretes MUC7 into the tear film. These soluble mucin in the tear film moves freely, (made possible by blinking and electrostatic repulsion from the negatively ...
The sustained delivery of both hydrophobic and hydrophilic drugs from hydrogels remains a challenge that requires the design of complex multifunctional synthetic polymers. In this work we exploit mucin glycoproteins, the gel-forming building blocks of natural mucus to deliver both hydrophobic and hydrophilic drugs. Mucins are a family of high molecular weight proteins which are densely glycosylated. Their central protein backbone contains hydrophobic and charged domains, while the mucin-associated glycans provide hydrogen bonding capabilities, high hydration, and negative charges. The biochemical versatility of mucins represents potential binding sites for certain drugs. If assembled into hydrogels, mucins could prevent drugs from freely diffusing out, leading to their sustained delivery. We generated methacrylated mucins which assembled into a covalently crosslinked mucin hydrogel when exposed to UV light. The rheological properties of the mucin gels were dominated by an elastic component, and ...
In the present study, we examined whether eosinophils induce MUC5AC mucin synthesis in airway epithelial cells. Our results showed that activated human eosinophils caused MUC5AC mucin synthesis in NCI-H292 cells. Similarly, the supernatant of activated eosinophils induced MUC5AC gene and protein synthesis in NCI-H292 cells, indicating that this effect of activated eosinophils was related to a secreted product.. Because EGFR activation is reported to cause mucin synthesis (13), we examined whether EGFR activation is required for activated eosinophil-induced mucin synthesis. Activated eosinophil supernatant induced EGFR phosphorylation in NCI-H292 cells, and selective inhibitors of EGFR tyrosine kinase blocked activated eosinophil-induced EGFR phosphorylation and MUC5AC synthesis completely; a selective PDGFR inhibitor (AG1295) and a negative control for tyrphostins (AG9) were without effect. These findings implicate EGFR tyrosine kinase phosphorylation in mucin synthesis induced by activated ...
Goblet cells reside throughout the gastrointestinal (GI) tract and are responsible for the production and preservation of a protective mucus blanket by synthesizing and secreting high molecular weight glycoproteins known as mucins. The concept of the mucus layer functioning as a dynamic protective barrier is suggested by studies showing changes in mucins in inflammatory conditions of the GI tract, by the altered goblet cell response in germ-free animals, and by the enhanced mucus secretion seen in response to infections. The mucin-containing mucus layer coating the GI epithelium is the front line of innate host defense. Mucins are likely to be the first molecules that invading pathogens interact with at the cell surface and thus, can limit binding to other glycoproteins and neutralize the pathogen. This review will focus on what is known about goblet cell response in various GI infections and the regulatory networks that mediate goblet cell function and mucin production in response to intestinal insults
TY - JOUR. T1 - Single molecule study of heterotypic interactions between mucins possessing the Tn cancer antigen. AU - Haugstad, Kristin E.. AU - Stokke, Bjørn T.. AU - Brewer, Curtis F.. AU - Gerken, Thomas A.. AU - Sletmoen, Marit. PY - 2015. Y1 - 2015. N2 - Mucins are linear, heavily O-glycosylated proteins with physiological roles that include cell signaling, cell adhesion, inflammation, immune response and tumorgenesis. Cancer-associated mucins often differ from normal mucins by presenting truncated carbohydrate chains. Characterization of the binding properties of mucins with truncated carbohydrate side chains could thus prove relevant for understanding their role in cancer mechanisms such as metastasis and recognition by the immune system. In this work, heterotypic interactions of model mucins that possess the Tn (GalNA-cαThr/Ser) and T (Galβ1-3GalNAcαThr/Ser) cancer antigens derived from porcine submaxillary mucin (PSM) were studied using atomic force microscopy. PSM possessing only ...
TY - JOUR. T1 - Interaction of bile salt and phospholipids with bovine submaxillary mucin. AU - Wiedmann, T. S.. AU - Deye, C.. AU - Kallick, D.. PY - 2001. Y1 - 2001. N2 - Purpose. The purpose of this study was to determine the distribution and diffusion of sodium taurocholate-phospholipid micelles with mucin in order to provide the foundation for understanding the transport of ingested fat and poorly water-soluble drugs through the intestinal mucous layer. Methods. Sodium taurocholate NaTC was dispersed with egg phosphatidylcholines PC to yield mixed micelles of a specific size and concentration. A preliminary study was conducted to determine the time required for equilibration of PC/TC micellar solutions with mucin. PC/TC micellar solutions were dialyzed against fixed and variable concentrations of bovine submaxillary mucin after which the concentration of PC and NaTC was measured by an assay for total phosphorus and by HPLC, respectively. In addition, a quantitative assay of TC and PC by ...
Figure 1. Yearly number of publication related to mucins as reported by scicurve.com, using the keyword mucins.. This is because mucin research spans over many fields. Biologists have been intrigued by mucins since long. But more recently, microbiologists studying our microbiome have new reasons to find this class of biopolymers particularly exciting. For instance, they realized that intestinal mucus plays key roles in the behavior of both pathogenic and commensal bacteria. This will surely lead to many more microbiology studies with mucus/mucins as a key component (1,2). Similarly, recent findings in immunology describing interactions between dendritic cells and mucin-associated glycans will also attract attention to secreted mucins in that field (3).. Some physicists and biophysicists are also fascinated by mucins. Tribologists are trying to understand and recreate mucins efficient lubricity that is so precious for a healthy mouth and gastrointestinal tract (4,5). The field of oral drug ...
The mucin-type glycoprotein podoplanin is specifically expressed by lymphatic but not blood vascular endothelial cells in culture and in tumor-associated lymphangiogenesis, and podoplanin deficiency results in congenital lymphedema and impaired lymphatic vascular patterning. indicated by granulosa cells in regular ovarian follicles highly, and by ovarian granulosa and dysgerminomas cell tumors. Although podoplanin was absent from regular human being epidermis mainly, its manifestation was induced in 22 of 28 squamous cell carcinomas studied strongly. These findings recommend a potential part of podoplanin in tumor development, plus they also determine the 1st commercially obtainable antibody for the precise staining of a precise lymphatic marker in archival human being tissue sections, allowing more widespread research of tumor lymphangiogenesis in human cancers thereby. Lymphatic vessels play a significant part in the maintenance of cells homeostasis1 and in the transportation of immune system ...
Mucins (/ˈmjuːsɪn/) are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by epithelial tissues in most animals.[1] Mucins key characteristic is their ability to form gels; therefore they are a key component in most gel-like secretions, serving functions from lubrication to cell signalling to forming chemical barriers.[1] They often take an inhibitory role.[1] Some mucins are associated with controlling mineralization, including nacre formation in mollusks,[2] calcification in echinoderms[3] and bone formation in vertebrates.[4] They bind to pathogens as part of the immune system. Overexpression of the mucin proteins, especially MUC1, is associated with many types of cancer.[5] Although some mucins are membrane-bound due to the presence of a hydrophobic membrane-spanning domain that favors retention in the plasma membrane, most mucins are secreted as principal components of mucus by mucous membranes or are secreted to become a component of saliva. ...
TY - JOUR. T1 - Pattern of mucin gene expression in normal and neoplastic lung tissues. AU - Seregni, E.. AU - Botti, C.. AU - Lombardo, C.. AU - Cantoni, A.. AU - Bogni, A.. AU - Cataldo, I.. AU - Bombardieri, E.. PY - 1996. Y1 - 1996. N2 - This work evaluates the expression in lung cancer of the most well characterized mucin genes (MUC1, MUC2, MUC3) and of the recently described MUC4 in lung tissues, to check a correlation between the expression of any particular gene and this tumor. Hybridization with synthetic oligonucleotides obtained from a part of the sequences of MUC1, MUC2, MUC3 and MUC4, was performed on blotted RNA from 18 lung cancer tissue specimens and from 10 normal tissues samples taken, when possible, from the normal lung counterpart. By means of Northern blot analysis MUC1 revealed to be the most expressed mucin gene in lung cancer, followed by MUC4; by contrast, the expression of MUC2 and MUC3 was almost undetectable in all cancer specimens. The intensity of expression of MUC1 ...
Liu, C., Comparison of structural characteristics of major membrane-bound mucins from porcine milk and intestinal brush borders. (2003). Dairy Science Publication Database. 336 ...
Salivary secretions contain two distinct mucins, a high molecular weight mucin named MG1 and a low molecular weight mucin named MG2. Mucins protect oral surface...
Mucins are high-molecular weight glycoproteins (0.25-20 MDa) containing one or more domains that are heavily O-glycosylated. Their implications as targets for cancer treatment have increased the interest in these glycoproteins, mainly in the fields of vaccines and antibodies. However, mucins present high heterogeneity, posing challenges that affect purification processes and quality control analysis. In that sense, it is necessary to develop and improve downstream processes and analytical methods to characterize these products. Here a tool based on biolayer interferometry analysis to improve mucins detection and quantification in a fast, simple and label free-way is presented. Taking advantage of lectin recognition of mucins carbohydrate structures, several lectins were evaluated and immobilized on streptavidin biosensors. Different assay conditions were optimized and the most suitable lectin, Aleuria aurantia lectin (AAL), was selected. Bovine Submaxillary Gland and human MUC5B mucins were ...
TY - JOUR. T1 - Enhanced self-association of mucins possessing the T and Tn carbohydrate cancer antigens at the single-molecule level. AU - Haugstad, Kristin E.. AU - Gerken, Thomas A.. AU - Stokke, Bjørn T.. AU - Dam, Tarun K.. AU - Brewer, C. Fred. AU - Sletmoen, Marit. PY - 2012/5/14. Y1 - 2012/5/14. N2 - Mucins are linear O-glycosylated glycoproteins involved in inflammation, cell adhesion, and tumorigenesis. Cancer-associated mucins often possess increased expression of the T (Galβ1,3GalNAcαThr/Ser) and Tn (GalNAcαThr/Ser) cancer antigens, which are diagnostic markers for several cancers, including colon cancer. We have used AFM based single-molecule forced unbinding under near physiological conditions to investigate the self-interactions between porcine submaxillary mucin (PSM) as well as between PSM analogs possessing various carbohydrates including the T- and Tn-antigen. Distributions of unbinding forces and corresponding force loading rates were determined for force loading rates ...
We have previously reported the establishment of cytotoxic T-cell lines from pancreatic cancer patients, by continuously stimulating tumor-draining lymph node cells with allogeneic pancreatic tumor cell lines. After the preliminary characterization of their phenotype and tumor specificity, detailed studies performed with one of the cell lines, W.D., show that it recognizes a specific antigen, a large and heavily glycosylated mucin molecule, expressed on pancreatic and breast tumors and tumor cell lines. Although this recognition appears major histocompatibility complex (MHC)-unrestricted, the antigen receptor used by the cytotoxic T cell is the alpha/beta heterodimer, typically found on MHC-restricted T cells. The target antigen is atypical, however, in its ability to directly bind and activate the T cells in the absence of self MHC, presumably by abundant and regularly repeated antigenic epitopes. These findings are important because they demonstrate a specific T-cell response against a human ...
LAA413Hu71, Biotin-Linked Polyclonal Antibody to Mucin 1 (MUC1), Homo sapiens (Human), Labelled antibody, CD227, EMA, H23AG, KL-6, PEM, PEMT, PUM, CA15-3, Cancer antigen 15-3, Carcinoma-associated mucin, Episialin, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Designed by Cloud-Clone Corp.
Mucins are high molar mass glycoproteins that assume an extended conformation and can assemble into mucus hydrogels that protect our mucosal epithelium. In nature, the challenging task of generating a mucus layer, several hundreds of micrometers in thickness, from micrometer-sized cells is elegantly solved by the condensation of mucins inside vesicles and their on-demand release from the cells where they suddenly expand to form the extracellular mucus hydrogel. We aimed to recreate and control the process of compaction for mucins, the first step toward a better understanding of the process and creating biomimetic in vivo delivery strategies of macromolecules. We found that by adding glycerol to the aqueous solvent, we could induce drastic condensation of purified mucin molecules, reducing their size by an order of magnitude down to tens of nanometers in diameter. The condensation effect of glycerol was fully reversible and could be further enhanced and partially stabilized by cationic ...
In gene-targeted mouse models for cystic fibrosis (CF), the disease is mainly manifested by mucus obstruction in the intestine. To explore the mucus composition, mucins insoluble and soluble in 6 M guanidinium chloride were purified by three rounds of isopycnic ultracentrifugation from the small and large intestines of CF mice (Cftr(m1UNC)/Cftr(m1UNC)) and compared with wild-type mice. The amino acid composition was typical of that for mucins and showed increased amounts of the insoluble (2.5-fold increase) and soluble (7-fold increase) mucins in the small intestine of the CF mice compared with wild-type mice. Mucins from the large intestine of both wild-type and CF mice showed a high but constant level of fucosylation. In contrast, the insoluble and soluble mucins of the small intestine in CF mice revealed a large increase in fucose, whereas those of wild-type mice contained only small amounts of fucose. This increased fucosylation was analysed by releasing the O-linked oligosaccharides ...
An unusual case of a mucin secreting benign microfollicular adenoma of the thyroid in a 30 year old euthyroid woman is reported. Histologically, the lesion was characterised by follicular cells with the appearance of signet ring cells. Histochemistry showed the mucin content of these cells to consist uniformly of sulphated acid mucins; positive thyroglobulin immunostaining was also shown. The published work on primary mucin secreting tumours of the thyroid gland is reviewed. Dual differentiation is thought to be responsible for combined mucin secretion and hormone production in this type of neoplasm.. ...
Efficient separation of mucins (200 kDa-2 MDa) was demonstrated using gradient SDS agarose/polyacrylamide composite gel electrophoresis (SDS-AgPAGE). Inclusion of urea (SDS-UAgPAGE) in the gels casting were shown to have no effect on the migration of mucins in the gel and allowed casting of gel at room temperature. This simplified the procedure for multiple casting of agarose polyacrylamide gradients and increased reproducibility of these gels. Hence, the implementation of urea makes the technique applicable for high throughput isolation and screening of mucin oligosaccharides by LC-MS after releasing the oligosaccharides from isolated, blotted mucin subpopulations. It was also shown that the urea addition had no effect on other supporting applications such as western and lectin blotting. In addition, identification of the mucin protein after tryptic digestion and LC-MS was possible and no protein carbamylation due to the presence of urea in the gel was detected. LC-MS software developed for ...
Author(s): Ren, Binhui; Azzegagh, Zoulikha; Jaramillo, Ana M; Zhu, Yunxiang; Pardo-Saganta, Ana; Bagirzadeh, Rustam; Flores, Jose R; Han, Wei; Tang, Yong-Jun; Tu, Jing; Alanis, Denise M; Evans, Christopher M; Guindani, Michele; Roche, Paul A; Rajagopal, Jayaraj; Chen, Jichao; Davis, C William; Tuvim, Michael J; Dickey, Burton F | Abstract: Airway mucin secretion is important pathophysiologically and as a model of polarized epithelial regulated exocytosis. We find the trafficking protein, SNAP23, selectively expressed in secretory cells compared to ciliated and basal cells of airway epithelium by immunohistochemistry and fluorescence activated cell sorting, suggesting that SNAP23 functions in regulated but not constitutive epithelial secretion. Heterozygous SNAP23 deletant mutant mice show spontaneous accumulation of intracellular mucin, indicating a defect in baseline secretion. However mucins are release from perfused tracheas of mutant and wild-type mice at the same rate, suggesting that increased
The selectin family of cell adhesion molecules mediates the tethering and rolling of leukocytes on blood vessel endothelium. It has been postulated that the molecular basis of this highly dynamic adhesion is the low affinity and rapid kinetics of selectin interactions. However, affinity and kinetic analyses of monomeric selectins binding their natural ligands have not previously been reported. Leukocyte selectin (L-selectin, CD62L) binds preferentially to O-linked carbohydrates present on a small number of mucin-like glycoproteins, such as glycosylation-dependent cell adhesion molecule-1 (GlyCAM-1), expressed in high endothelial venules. GlyCAM-1 is a soluble secreted protein which, following binding to CD62L, stimulates beta2-integrin-mediated adhesion of lymphocytes. Using surface plasmon resonance, we show that a soluble monomeric form of CD62L binds to purified immobilized GlyCAM-1 with a dissociation constant (Kd) of 108 microM. CD62L dissociates from GlyCAM-1 with a very fast dissociation rate
Latest reports claim that autoantibodies directed to glycosylated mucins aberrantly, specifically MUC4 and MUC1, are located in individuals with colorectal cancer. Rosetta2 (Novagen), nickel purified using NiNTA agarose (Qiagen, Venlo, NL) accompanied by HPLC purification before and after O-glycosylation. Items were analyzed by MALDI-TOF and SDS-PAGE mass spectrometry on the Voyager-DE? PRO workstation (Applied Biosystems, Lifestyle Technology, Carlsbad, CA) using 2,5-dihydroxybenzoic acidity (Sigma, St. Louis, Missouri) as matrix. AB1010 Microarray MUC1, MUC4 and MUC2 glycopeptides and glycoproteins were printed on Schott Nexterion? Glide H MPX 48 (Schott AG, Mainz, Germany) as previously defined.18 In brief, triplicates of most peptides, in 150 mM sodium phosphate pH 8.5 with 0.005% CHAPS, were printed on the BioRobotics MicroGrid II spotter (Genomics Solution) having a 0.21-mm pitch using Stealth 3B Micro Spotting Pins (Telechem International ArrayIt Division). After printing, slides had been ...
TY - JOUR. T1 - Mechanistic and functional shades of mucins and associated glycans in colon cancer. AU - Pothuraju, Ramesh. AU - Krishn, Shiv Ram. AU - Gautam, Shailendra K.. AU - Pai, Priya. AU - Ganguly, Koelina. AU - Chaudhary, Sanjib. AU - Rachagani, Satyanarayana. AU - Kaur, Sukhwinder. AU - Batra, Surinder K.. PY - 2020/3. Y1 - 2020/3. N2 - Mucus serves as the chief protective barrier against pathogenic and mechanical insults in respiratory, gastrointestinal, and urogenital tracts. Altered mucin expression, the major component of mucus, in conjunction with differential glycosylation has been strongly associated with both benign and malignant pathologies of colon. Mucins and their associated glycans arbitrate their impact sterically as well as mechanically by altering molecular and microbial spectrum during pathogenesis. Mucin expression in normal and pathological conditions is regulated by nonspecific (dietary factors and gut microbiota) and specific (epigenetic and transcriptional) ...
To investigate the genetic identities of the mucins secreted in cystic fibrosis (CF) airways, sputum was collected from five individuals. Samples were separated into gel and sol phases by high-speed centrifugation and the gel phase was extracted in 6 M guanidinium chloride. The insoluble residue remaining after extraction of the gel phase was brought into solution by reduction/alkylation. Density-gradient centrifugation in CsCl revealed polydisperse distributions of sialic acid-containing mucins in the gel phase, insoluble residue and sol phase fractions and the degree of variation between the different individuals was low. Antibodies recognizing MUC5AC and MUC5B identified these mucins in each of the fractions. MUC2, however, was present only as a component of the insoluble residue from the gel which accounted for less than 4% by mass of the total mucins. MUC5B and MUC5AC from the gel phase were large oligomeric species composed of disulphide-bond linked subunits and MUC5B was present as two ...
Mucins are the primary macromolecular component of mucus-natures natural lubricant-although they are poorly characterised heterogeneous substances. Recent advances in hydrodynamic methodology now offer the opportunity for gaining a better understanding of their solution properties. In this study a combination of such methods was used to provide increased understanding of a preparation of porcine intestinal mucin (PIM), MUC2 mucin, in terms of both heterogeneity and quantification of conformational flexibility. The new sedimentation equilibrium algorithm SEDFIT-MSTAR is applied to yield a weight average (over the whole distribution) molar mass of 7.1 × 106 g mol−1, in complete agreement with size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS), which yielded a value of 7.2 × 106 g mol−1. Sedimentation velocity profiles show mucin to be very polydisperse, with a broad molar mass distribution obtained using the Extended Fujita algorithm, consistent with the ...
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The aim of this study was to assess the profile of mucins (MUC1, MUC2, MUC5AC) in the intestinal metaplasia (IM) of the gastric mucosa through the immunohistochemical method.. Methods: To identify the metaplastic areas in the gastric mucosa, chromoendoscopy was employed using 0.5% solution of methylene blue. The expression of the profile of the mucins was determined using immunohistochemistry with MUC1, MUC5AC, and MUC2 antibodies (clone Ma695, clone CLH2, Ccp58 and CLH5, Novocastra Great Britain).. Results: In the regions adjacent to the adenocarcinoma and neoplastic modified cells, a visible weak expression of MUC2 and MUC5AC was observed. In the case of complete IM, a visibly maximum MUC2 expression was observed in the goblet cells; thus, the MUC5AC, MUC1, and MUC6 marking were absent in the columnar epitheliocytes with the brush border. In the case of incomplete IM, along with the positive MUC2 markings of the goblet cells, the presence of gastric mucin (MUC5AC) has been observed in 25% of ...
Looking for mucin clot test? Find out information about mucin clot test. see glycoprotein glycoprotein , organic compound composed of both a protein and a carbohydrate joined together in covalent chemical linkage. These... Explanation of mucin clot test
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From repairing skin damage and providing anti-aging benefits to soothing irritated skin and fading acne scars, snail mucin, or snail secretion filtrate, offers so many benefits. Its truly a versatile ingredient, suitable for all skin types and concerns, and plays well with other ingredients. Studies have shown that snail mucin even helps with atopic dermatitis. In fact, even before snail mucin became popular as a skin care ingredient, the main component of snail mucin, Chondroiton sulfate, was recognized for its ability to help improve arthritis, liver diseases, nephritis, and fatigue.. But have you ever wondered where snail mucin comes from? You might be thinking that snails are plucked from their natural habitats and forced to produce mucin, which eventually ends up in pretty packaging on store shelves. Or you might have assumed that snail mucin is artificially created in a petri dish and then mass produced to meet the high demand for snail mucin. The answer is none of the above. Read on for ...
MUC5AC (mucin 5AC, oligomeric mucus/gel-forming), Authors: Raquel Mejías-Luque, Lara Cobler, Carme de Bolós. Published in: Atlas Genet Cytogenet Oncol Haematol.
TY - JOUR. T1 - Mucins in pancreatic cancer and its microenvironment. AU - Kaur, Sukwinder. AU - Kumar, Sushil. AU - Momi, Navneet. AU - Sasson, Aaron R.. AU - Batra, Surinder Kumar. PY - 2013/10/1. Y1 - 2013/10/1. N2 - Pancreatic cancer remains a lethal malignancy with poor prognosis owing to therapeutic resistance, frequent recurrence and the absence of treatment strategies that specifically target the tumour and its supporting stroma. Deregulated cell-surface proteins drive neoplastic transformations and are envisioned to mediate crosstalk between the tumour and its microenvironment. Emerging studies have elaborated on the role of mucins in diverse biological functions, including enhanced tumorigenicity, invasiveness, metastasis and drug resistance through their characteristic O-linked and N-linked oligosaccharides (glycans), extended structures and unique domains. Multiple mucin domains differentially interact and regulate different components of the tumour microenvironment. This Review ...
Mucin 1 (MUC1) is a heterodimeric protein that is aberrantly expressed by diverse human carcinomas and certain hematological malignancies (Kufe, 2009). The overexpression of MUC1, as found in human cancers, is associated with the induction of anchorage-independent growth and tumorigenicity (Li et al., 2003). Based on these findings, MUC1 has emerged as an attractive target for the development of anticancer agents. However, the identification of drugs that block MUC1 has been limited by the lack of sufficient information regarding how MUC1 contributes to the growth and survival of malignant cells. In this regard, the MUC1 protein is translated by a single mRNA and then undergoes autocleavage into two subunits that in turn form a heterodimer (Kufe, 2009). The MUC1 N-terminal subunit is the mucin component of the heterodimer that contains the characteristic glycosylated tandem repeats and is expressed on the cell surface in a complex with the MUC1 C-terminal transmembrane subunit (MUC1-C) (Kufe, ...
TFF-2, human recombinant protein, Trefoil Factor 2, Spasmolytic polypeptide (SP), Spasmolysin validated in (PBV10395r-100), Abgent
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Natural fragmentation of polypeptide chains by autoproteolysis occurs in a number of protein families. It is a vital step in the maturation of several enzymes and in the formation of membrane-associated mucins that constitute a part of the protective mucus barrier lining epithelial cells. These reactions follow similar routes involving an initial N-O or N-S acyl shift starting with a nucleophilic attack by a hydroxyl or thiol group on a carbonyl carbon followed by resolution of the ester intermediate. Previous studies indicate that distortion of the scissile peptide bond may play a role in autoproteolysis. Our structural, biochemical and molecular dynamics studies of the autoproteolyzed SEA domains from human membrane-bound mucin MUC1 and human orphan receptor GPR116 confirmed this by revealing a novel biochemical mechanism where the folding free energy accelerates cleavage by imposing conformational strain in the precursor structure. This mechanism may well be general for autoproteolysis. The ...
臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。. To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of NTU Repository with Academic Hub to form NTU Scholars.. ...
Extraction, characterization, and use of mucin biopolymers for biomedical applications.. For thousands of years, and still today, humans have exploited natural materials for their benefit. For instance, wood-extracted cellulose fibers are used to make paper and animal-extracted hyaluronic acid is used in cosmetic anti wrinkle creams. In this proposal we will develop the use of mucins, i.e. a molecules extracted from mucus, for biomedical applications. Mucins are the main components of mucus, the gel that covers the wet epithelium of all mammals. Commonly secreted by animal tissues, they can be extracted from abundant sources such as pig stomachs and bovine submaxillary glands.. The study and technological use of mucins are hampered by the poor control over the quality of the mucins available. In this proposal, we first aim to develop a quality-control benchmark by measuring a series of physical and biochemical properties. Using this benchmark, we will then identify new processes to efficiently ...
TY - JOUR. T1 - Trefoil factor(s) and CA19.9. T2 - A promising panel for early detection of pancreatic cancer. AU - Jahan, Rahat. AU - Ganguly, Koelina. AU - Smith, Lynette M.. AU - Atri, Pranita. AU - Carmicheal, Joseph. AU - Sheinin, Yuri. AU - Rachagani, Satyanarayana. AU - Natarajan, Gopalakrishnan. AU - Brand, Randall E.. AU - Macha, Muzafar A.. AU - Grandgenett, Paul M.. AU - Kaur, Sukhwinder. AU - Batra, Surinder K.. PY - 2019/4. Y1 - 2019/4. N2 - Background: Trefoil factors (TFF1, TFF2, and TFF3) are small secretory molecules that recently have gained significant attention in multiple studies as an integral component of pancreatic cancer (PC) subtype-specific gene signature. Here, we comprehensively investigated the diagnostic potential of all the member of trefoil family, i.e., TFF1, TFF2, and TFF3 in combination with CA19.9 for detection of PC. Methods: Trefoil factors (TFFs) gene expression was analyzed in publicly available cancer genome datasets, followed by assessment of their ...
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Introduction: Wood smoke (WS) exposure is associated with increased prevalence of chronic bronchitis and rapid decline in lung function. However, potency of WS and cigarette smoke (CS) in activating mucin gene expression has not been compared.. Aims and objectives: We tested the hypothesis that WS is more potent than CS in causing mucous cell metaplasia (MCM).. Methods: C57BL/6 mice were exposed for 30 d to filtered air (FA) or CS for 5 days/week and 6 hours/day at 250 mg/m3 total particulate matter (TPM) or to WS for 2 h/d at 10 mg TPM/m3. Extent of MCM was determined in stained lung sections. Mucin gene expression was analyzed by Western blot, qRT-PCR, and immunofluorescent staining. Findings were replicated in differentiated primary airway epithelial cultures from human (HAEC) and murine cells (MAECs).. Results: WS exposure at 25-fold lower TPM concentrations than CS increased Muc5ac, Muc5b, SPDEF and Bcl-2 mRNAs in the lungs of mice, while these mRNAs were not significantly increased by FA ...
Beatson, Richard, Tajadura-Ortega, Virginia, Achkova, Daniela, Picco, Gianfranco, Tsourouktsoglou, Theodora-Dorita, Klausing, Sandra, Hillier, Matthew, Maher, John, Noll, Thomas, Crocker, Paul R, Taylor-Papadimitriou, Joyce, and Burchell, Joy M. The mucin MUC1 modulates the tumor immunological microenvironment through engagement of the lectin Siglec-9. Nature Immunology 17.11 (2016): 1273-1281 ...
TY - JOUR. T1 - Chromosomal Localization of a Human Mucin Gene (MUC8) and Cloning of the cDNA Corresponding to the Carboxy Terminus. AU - Shankar, Viswanathan. AU - Pichan, Prabhasankar. AU - Eddy, Roger L.. AU - Tonk, Vijay. AU - Nowak, Norma. AU - Sait, Sheila N J. AU - Shows, Thomas B.. AU - Schultz, Roger E.. AU - Gotway, Garrett. AU - Elkins, Ronald C.. AU - Gilmore, Michael S.. AU - Sachdev, Goverdhan P.. PY - 1997. Y1 - 1997. N2 - A partial cDNA (pAM1) encoding a major airway mucin glycoprotein with novel tandem repetitive sequence has recently been cloned. In this article, we report additional new sequence derived by 3′-rapid amplification of cDNA ends technique. The sequence corresponds to a stop codon, 3′-untranslated region of 458 bp, a polyadenylation signal, and poly A+ tail, and represents the extreme carboxy terminus of MUC8. A plasmid construct (pAM3) in pBluescript was generated by in-frame ligation of pAM1 to the 479-bp 3′UTR of MUC8. A 5′-end 325-bp fragment of this ...
1. Human gallbladder mucin has been implicated to play a role in gallstone disease. In spite of this fact relatively little is known about the structure of human gallbladder mucin. In this study we have investigated the possible heterogeneity of mucin. For this purpose polyclonal and monoclonal antibodies against gallbladder mucin were raised. All antibodies reacted primarily with carbohydrate antigenic determinants. With these antibodies the immunoreactivity of gallbladder mucin from 60 patients with cholesterol gallstones and 20 subjects without stones was screened. In addition, reactivity with several lectins was studied.. 2. Considerable heterogeneity was found with both antibody and lectin typing, but there was no significant difference in heterogeneity between mucin from patients with gallstones and control subjects. Immunoblotting revealed that there was similarity between the reaction of the polyclonal antibody and the Helix pomatia agglutinin. All mucin preparations reacting with the ...
The commensal bacteria in colon live and thrive in the outer loose mucus layer. This is possible after the Muc2 mucin network has expanded in volume, such that it allows the bacteria to penetrate into the mucin network. Once inside the mucus gel, the commensal bacteria can use its large number of glycan-degrading enzymes that release one monosaccharide at a time from the mucin glycans. In this way, it will take some time for the bacterial enzymes to reach and expose the mucin protein core for proteolysis that will degrade the mucin protein core. In this way the mucin polymeric network of the loose mucus is maintained for some time to give a relatively thick outer mucus layer. Although difficult to visualize, the outer loose mucus will probably be increasingly degraded the closer it comes to the luminal content. It is further indicated that bacteria contribute to dissolving the outer loose mucus as germ-free mice have, relative to the inner layer, a thick outer loose mucus layer (16). As ...
Purpose : Terminally differentiated stratified epithelial cells maintain barrier function at the ocular surface through a specialized protective structure composed of highly O-glycosylated transmembrane mucins. Relatively low levels of N-glycans are found on transmembrane mucins, and their structure and function remains poorly characterized. The purpose of this study was to define the contribution of mucin N-glycans to the ocular surface glycocalyxl barrier. Methods : Transmembrane mucins were purified from stratified corneal epithelial cell cultures using size exclusion chromatography and isopycnic density centrifugation. N-glycans released from mucin isolates were analyzed by liquid chromatography and electrospray ionization mass spectroscopy. N-glycosylation was inhibited using chemical, enzymatic and siRNA (MGAT1) approaches. Plasma membrane proteins were labeled by biotinylation. Lectin blot, immunoblot, and qPCR were used to determine levels of carbohydrate, protein, and mRNA, ...
Abstract: : Purpose: Our previous investigations proved that conjunctival impression cytology (CIC) is a valid, non-invasive technique that is useful to detect mRNA levels of ocular mucin genes in healthy individuals. This study was designed to perform the relative quantitation of MUC1, 2, 4, 5AC and 7 mucin genes in CIC samples from healthy males and females, younger and older of 60 years of age. Methods: Two polyethersulfone filters were applied to the superior bulbar conjunctiva of 38 healthy donors. Two age categories were considered: less that 60 years (7 males, 7 females), and 60 years or older (15 males, 9 females). Real time RT-PCR was performed using total RNA to examine the expression of MUC1, 2, 4, 5AC and 7. Their relative quantity was determined by SYBR® Green I dye detection. The housekeeping gene GAPDH was used to normalize the amount of expression levels. Data of mucin genes were analyzed using a logarithmic transformation to obtain a distribution closer to the normal ...
Mucin glycoproteins play a key role in the normal function of the airway epithelium. We examined the expression of mucin genes, MUC3, 4, 5AC, 5B, 6, 7, and 8 in human fetal tissues to establish the localization and age of onset of expression of each mucin gene during human development. We detected expression of MUC4, 5AC, 5B, and 7 in the mid-trimester airway epithelium but did not detect expression of MUC3, 6, or 8. MUC4 was expressed in the trachea and large airways in the majority of cells in the airway epithelium. Expression of MUC5AC was only seen in individual goblet cells in the trachea, while MUC5B was expressed in the surface epithelium of the trachea at 13 wk but was largely restricted to submucosal glands by 23 wk of gestation.
TY - JOUR. T1 - Mucin expression profile in Barretts, dysplasia, adenocarcinoma sequence in the esophagus. AU - Burjonrappa, S. C.. AU - Reddimasu, S.. AU - Nawaz, Z.. AU - Gao, X.. AU - Sharma, Poonam K. AU - Loggie, Brian W.. PY - 2007/1/1. Y1 - 2007/1/1. N2 - BACKGROUND: The molecular events that accompany the progression to adenocarcinoma (ADC) of the esophagus are poorly understood. Aberrant mucin receptor expression can contribute to increased cell growth and metastatic ability. AIM: The aim of this study was to establish a pattern for mucin (MUC) gene expression in the esophageal mucosa under normal and pathological conditions. SETTING: University Hospital Cancer Center Laboratory. Archived tissue samples studied in a retrospective fashion. MATERIALS AND METHODS: Tissue samples were obtained from the archives of patients with histological evidence of Barretts esophagus (BE) progressing to ADC. Immunohistochemical analysis was performed using mouse monoclonal antibodies for MUC1, MUC2, ...
Changes in the expression of mucin genes in gastrointestinal cancers is thought to contribute to the development of the disease. In our laboratory we have shown previously that MUC5AC is aberrantly expressed in rectosigmoid villous adenomas. However, the regulatory mechanisms underlying that altered profile of expression is unknown. In order to study its regulation at the transcriptional level, we have isolated and characterized 5.5 kb of the 5´-flanking region of the mouse Muc5ac mucin gene. The promoter is flanked by a TATA box and a transcriptional start site is located 22 bp downstream of the TATA box. Analysis of the sequence showed a high density of binding sites for Smad4, an essential factor in the signalling cascade activated by TGF-β (transforming growth factor-β), and Sp1, an important factor in the regulation of MUC5AC. This led us to study Muc5ac regulation by TGF-β. We show that exogenous addition of TGF-β to the cells induces Muc5ac endogenous expression, promoter activity ...
TY - JOUR. T1 - Molecular basis of antibody binding to mucin glycopeptides in lung cancer. AU - Qu, Jin. AU - Yu, Hongtao. AU - Li, Fenge. AU - Zhang, Chunlei. AU - Trad, Ahmad. AU - Brooks, Cory. AU - Zhang, Bin. AU - Gong, Ting. AU - Guo, Zhi. AU - Li, Yunsen. AU - Ragupathi, Govind. AU - Lou, Yanyan. AU - Hwu, Patrick. AU - Huang, Wei. AU - Zhou, Dapeng. PY - 2016/2. Y1 - 2016/2. N2 - Glycopeptides bearing Tn epitopes are emerging targets for cancer diagnosis and immunotherapy. In this study, we analyzed membrane proteins containing O-glycosylated tandem repeat (TR) sequences in lung cancer patients of different types and stages, using gene microarray data in public domain. The expression of Tn and glycopeptide epitopes on the surface of lung cancer cell lines were studied by monoclonal IgG antibodies 14A, 16A, and B72.3. The binding of mAbs to synthetic glycopeptides were studied by surface plasmon resonance. Nine mucin mRNAs were found to be expressed in lung cancer patients but at similar ...
Mucous membranes are covered with mucus, a viscoelastic hydrogel that plays an essential role in their protection from shear and pathogens. The viscoelasticity of mucus is owing to mucins, a group of densely glycosylated proteins. Mucins can interact with a wide range of surfaces; thus, there is big interest in exploring and manipulating such interactions for biomedical applications. This thesis presents investigations of mucin interactions with hydrophobic surfaces in order to identify the key features of mucin lubricity, as well as describes the development of materials that are optimized to interact with mucins.. In Paper I we investigated the domains which make mucins outstanding boundary lubricants. The results showed that the hydrophobic terminal domains of mucins play a crucial role in the adsorption and lubrication on hydrophobic surfaces. Specifically, protease digestion of porcine gastric mucins and salivary mucins resulted in the cleavage of these domains and the loss of lubricity and ...
O-glycans are a class of glycans that modify serine or threonine residues of proteins. Biosynthesis of O-glycans starts from the transfer of N-acetylgalactosamine (GalNAc) to serine or threonine. The first GalNAc may be extended with sugars including galactose, N-acetylglucosamine, fucose, or sialic acid, but not mannose, glucose, or xylose. Depending on the sugars added, there are four common O-glycan core structures, cores 1 through 4, and an additional four, cores 5 though 8. Mucins are highly O-glycosylated glycoproteins ubiquitous in mucous secretions on cell surfaces and in body fluids. Mucin O-glycans can be branched, and many sugars or groups of sugars are antigenic. Important modifications of mucin O-glycans include O-acetylation of sialic acid and O-sulfation of galactose and N-acetylglucosamine ...
O-glycans are a class of glycans that modify serine or threonine residues of proteins. Biosynthesis of O-glycans starts from the transfer of N-acetylgalactosamine (GalNAc) to serine or threonine. The first GalNAc may be extended with sugars including galactose, N-acetylglucosamine, fucose, or sialic acid, but not mannose, glucose, or xylose. Depending on the sugars added, there are four common O-glycan core structures, cores 1 through 4, and an additional four, cores 5 though 8. Mucins are highly O-glycosylated glycoproteins ubiquitous in mucous secretions on cell surfaces and in body fluids. Mucin O-glycans can be branched, and many sugars or groups of sugars are antigenic. Important modifications of mucin O-glycans include O-acetylation of sialic acid and O-sulfation of galactose and N-acetylglucosamine ...
TY - JOUR. T1 - IL-13 induces mucin production by stimulating epidermal growth factor receptors and by activating neutrophils. AU - Shim, Jae Jeong. AU - Dabbagh, Karim. AU - Ueki, Iris F.. AU - Dao-Pick, Trang. AU - Burgel, Pierre Regis. AU - Takeyama, Kiyoshi. AU - Tam, Dominic Cheng Wei. AU - Nadel, Jay A.. PY - 2001/1. Y1 - 2001/1. N2 - Mucus hypersecretion contributes to the morbidity and mortality in acute asthma. Both T helper 2 (Th2) cytokines and epidermal growth factor receptor (EGFR) signaling have been implicated in allergen-induced goblet cell (GC) metaplasia. Present results show that a cascade of EGFR involving neutrophils is implicated in interleukin (IL)-13-induced mucin expression in GC. Treatment with a selective EGFR tyrosine kinase inhibitor prevented IL-13-induced GC metaplasia dose dependently and completely. Instillation of IL-13 also induced tumor necrosis factor-α protein expression, mainly in infiltrating neutrophils. Control airway epithelium contained few ...
TY - JOUR. T1 - Mucin staining is of limited value in addition to basic immunohistochemical analyses in the diagnostics of non-small cell lung cancer. AU - Micke, Patrick. AU - Botling, Johan. AU - Mattsson, Johanna Sofia Margareta. AU - Planck, Maria. AU - Tran, Lena. AU - Vidarsdottir, Halla. AU - Nodin, Björn. AU - Jirström, Karin. AU - Brunnström, Hans. PY - 2019/12/1. Y1 - 2019/12/1. N2 - Accurate diagnosis of histological type is important for therapy selection in lung cancer. Immunohistochemical (IHC) and histochemical stains are often used to complement morphology for definite diagnosis and are incorporated in the WHO classification. Our main aim was to compare different mucin stains and assess their value in relation to common IHC analyses in lung cancer diagnostics. Using tissue microarrays from 657 surgically treated primary lung cancers, we evaluated the mucin stains periodic acid-Schiff with diastase (PASD), alcian blue-periodic acid-Schiff (ABPAS) and mucicarmine, and compared ...
Background: Repair of bone damage represents a fundamental issue in the treatment of periodontitis. The important indicator employed to monitor the bone damage repair process is the number of osteoblast cells. Achatina Fulica snail mucin (SM) contains glycosaminoglycans which have the potential to increase their number. However, the use of SM in dentistry remains limited. Purpose: To determine and prove the effect of SM gel in increasing the number of osteoblasts in rat models suffering from periodontitis. Methods: This study used 36 rat models divided into three groups, namely; a treatment group (T: 20% snail mucin gel, n = 12), a positive-control group (P: hyaluronic acid gel, n = 12) and a negative-control group (N: CMC-Na gel, n = 12). 0.2 ml of all material was applied to a pocket by means of a tuberculin syringe once a day for 14 days. Histologic observations using Haematoxylin-Eosin staining were carried out on days 3, 5, 7 and 14. Data was analyzed by two-way ANOVA followed by a post-hoc LSD.
The present study was undertaken to analyze the role of the MUC4 mucin in pancreatic tumor development and progression. Mucins are generally known to protect surface epithelia from injuries and infections under normal physiological conditions but often display deregulated expression in malignant cells (4 , 6 , 10) . We have previously reported that the human mucin gene MUC4 is aberrantly expressed in pancreatic tumors and cell lines but has no detectable expression in the normal pancreas (10) . The present in vitro and in vivo data reveal that the silencing of MUC4 expression results in altered tumor cell behavior, decreased growth, and a marked reduction in metastatic incidences in an orthotopic mouse model of pancreatic cancer. These results are in support of the previous observations and establish a direct association of MUC4 expression with the disease development.. Down-regulating the expression is one of the convenient and reliable approaches for functional analyses of genes (reviewed in ...
MUC5AC (Mucin 5AC / Gastric Mucin) Antibody, Mouse Monoclonal Antibody [Clone MUC5AC/917 ] validated in IHC, IF, FC (AH11914-7), Abgent
The MUC-1 antigen is a membrane-anchored mucin-type glycoprotein present in malignant and normal epithelial cells of certain organs, e.g. breast, lung, ovary, pancreas and colon (1). The apoprotein of the MUC-1 mucin contains a transmembrane domain, a cytoplasmic domain, and an extracellular carbohydrate rich domain. The extracellular domain is characterized by polymorphism with respect to the number of a 20 amino acid tandem repeat (VNTR polymorphism). The CanAg CA15-3 EIA is based on two mouse monoclonal antibodies, Ma695 as catcher antibody recognizing a sialylated carbohydrate epitope expressed on the MUC-1 antigen and Ma552 as tracer antibody targeting the PDTRPAPG region of the protein core (2-5).The MUC-1 breast cancer mucin (CA15-3 antigen) is secreted from tumor cells and is a well-established serological marker for monitoring the clinical course of breast cancer patients (6). ...
This graph shows the total number of publications written about Gastric Mucins by people in this website by year, and whether Gastric Mucins was a major or minor topic of these publications ...
Trousseau described spontaneous, recurrent superficial migratory thrombophlebitis associated with occult cancers, and this was later correlated with disseminated microangiopathy (platelet-rich clots in small blood vessels). Trousseau syndrome often occurs with mucinous adenocarcinomas, which secrete abnormally glycosylated mucins and mucin fragments into the bloodstream. Since carcinoma mucins can have binding sites for selectins, we hypothesized that selectin-mucin interactions might trigger this syndrome. When highly purified, tissue-factor free carcinoma mucin preparations were intravenously injected into mice, platelet-rich microthrombi were rapidly generated. This pathology was markedly diminished in P- or L-selectin-deficient mice. Heparin (an antithrombin-potentiating agent that can also block P- and L-selectin recognition of ligands) ameliorated this platelet aggregation, but had no additional effect in P- or L-selectin-deficient mice. Inhibition of endogenous thrombin by recombinant ...
Healthy individuals have few goblet cells in their airways, but in patients with hypersecretory diseases goblet-cell upregulation results in mucus hypersecretion, airway plugging, and death. Multiple stimuli produce hypersecretion via epidermal growth factor receptor (EGFR) expression and activation, causing goblet-cell metaplasia from Clara cells by a process of cell differentiation. These cells are also believed to be the cells of origin of non-small-cell lung cancer, but this occurs via cell multiplication. The mechanisms that determine which pathway is chosen are critical but largely unknown. Although no effective therapy exists for hypersecretion at present, the EGFR cascade suggests methods for effective therapeutic intervention.
TY - JOUR. T1 - Clinical presentation of mucin-secreting tumors of the pancreas. AU - Tibayan, Frederick (Fred). AU - Vierra, Mark. AU - Mindelzun, Bob. AU - Tsang, Don. AU - McClenathan, James. AU - Young, Harvey. AU - Trueblood, H. Ward. PY - 2000/5/15. Y1 - 2000/5/15. N2 - Background: Pancreatitis and jaundice secondary to ductal obstruction are common in intraductal papillary mucinous tumors (IPMT) of the pancreas. However, the incidence and severity of the complications of obstruction are not well documented. The aim of the study was to investigate the clinical presentation and outcome of 10 patients with IPMT. Methods: All cases of IPMT diagnosed between 1994 and 1999 were reviewed. Results: Four of the 10 patients developed severe acute illness with systemic complications resulting from ductal obstruction. Three suffered acute cholangitis with sepsis, and 1 developed necrotizing pancreatitis and ARDS. There was 1 postoperative death in a patient with adenocarcinoma. All other patients are ...
Experiment with focus on mucin. In 2009 Yana Znamenskaya Falk moved to Sweden for a PhD study at Malmö University. In her PhD Yana chose to focus on the effect of hydration on the structural, thermodynamic and rheological properties of biopolymer mucin, which is a main component of mucus. Mucus is a slippery secretion produced by, and covering, mucous membranes. Yana is currently working at Biofilms, Research Center for Biointerfaces, at Malmö University where she has continued her research study on mucin. In 2016 Yana participated in an experiment project within MAX4ESSFUN.. Yana explains that mucus consists primarily of water (95%), the glycoprotein mucin and other components and it can be found in all internal tracts of the body, f. ex. the mouth, nose, throat, sinuses, stomach and lungs. One of the essential functions of the mucous gel is protection of tissues against dehydration (drying). In order to fulfil this function, the mucous gel should be strongly hydrated, i.e. it should contain ...
We reported recently that interleukin (IL)-1β exposure resulted in a prolonged increase in MUC5AC mucin production in normal, well differentiated, human tracheobronchial epithelial (NHTBE) cell cultures, without significantly increasing MUC5AC mRNA (Am J Physiol 286:L320-L330, 2004). The goal of the present study was to elucidate the signaling pathways involved in IL-1β-induced MUC5AC production. We found that IL-1β increased cyclooxygenase-2 (COX-2) mRNA expression and prostaglandin (PG) E2 production and that the COX-2 inhibitor celecoxib suppressed IL-1β-induced MUC5AC production. Addition of exogenous PGE2 to NHTBE cultures also increased MUC5AC production and IL-1β-induced Muc5ac hypersecretion in tracheas from wild-type but not from COX-2-/- mice. NHTBE cells expressed all four E-prostanoid (EP) receptor subtypes and misoprostol, an EP2 and EP4 agonist, increased MUC5AC production, whereas sulprostone, an EP1 and EP3 agonist, did not. Furthermore, specific protein kinase A (PKA) ...
Mucin production by human colonic carcinoma cells correlates with their metastatic potential in animal models of colon cancer metastasis.: Patients with mucinou
A lambda gt11 cDNA library constructed from human tracheo-bronchial mucosa was screened with a polyclonal antiserum raised to chemically deglycosylated pronase glycopeptides from human bronchial mucins. Out of 20 positives clones, one partial cDNA clone was isolated and allowed to map a novel human …
A slimy layer of mucus serves as the first line of defense against problematic microbes like the bacterial pathogen Pseudomonas aeruginosa.. We have identified mucins, the major gel-forming components of mucus, and their complex sugar structures (glycans) as protective molecules that suppress microbial virulence traits including toxin secretion, bacterial communication, and surface attachment.. This virulence reduction is associated with less host cell killing in vitro (Figure) and less colonization of burn wounds.. Significance: By attenuating the virulence of pathogens rather than eradicating them, mucin glycans are attractive candidates for novel therapeutics.. ...
TY - JOUR. T1 - Distinctive roles of vitamin A in the regulation of mucin-like glycoprotein and keratin protein synthesis in cultured rabbit tracheal epithelial cells. AU - Wu, Reen. PY - 1982. Y1 - 1982. UR - http://www.scopus.com/inward/record.url?scp=0020465996&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0020465996&partnerID=8YFLogxK. M3 - Article. AN - SCOPUS:0020465996. VL - 95. JO - Journal of Cell Biology. JF - Journal of Cell Biology. SN - 0021-9525. IS - 2 II. ER - ...
Objectives. To characterise mucins in cancer of the colon and compare these with controls using stringent biochemical measures to avoid endogenous proteolysis. Design. Crude mucus scrapings were collected from 12 specimens obtained by colectomy. Specimens from 3 traumatic colectomies and 1 sigmoid volvulus were used as controls, and compared with 6 specimens from colons resected for adenocarcinoma and 2 irradiated colons. Subjects. The median age of the 4 female patients was 76 years (range 49 - 82 years), and of the 8 male patients 46.5 years (range 16 - 74 years). Results and conclusions. The crude mucus scrapings in the 9 specimens ranged in weight from 353 mg to 7 697 mg (median 4 928 mg). The median of purified mucin in the 9 specimens was 0.72 mg/mg wet weight of scraped material. Eight samples gave non-extractable pellet material, and were treated with DTT to reduce disulphide bonds for further analysis. One of these 8 pellets was resistant to reduction and had to be digested with papain
Acute kidney injury (AKI) arises from multiple forms of renal damage, including ischemia, toxin exposure, and urinary tract obstruction. AKI-associated inflammation exacerbates damage and can lead to development of chronic kidney disease (CKD). Kidney injury molecule-1 (KIM-1) is a mucin and immunoglobulin domain-containing transmembrane protein that is highly upregulated in the proximal tubule after kidney injury. While chronic KIM-1 expression promotes renal fibrosis and kidney failure, Li Yang, Craig Brooks, and colleagues at Harvard Medical School reveal that KIM-1 dampens early inflammatory responses and protects the kidney following injury. Mice expressing a mutant form of KIM-1 that lacks the mucin domain (KIM-1Δmucin) were more susceptible to both ischemia-induced and toxin-induced AKI compared to control animals. Loss of the mucin domain disrupted KIM-1-mediated phagocytosis in proximal tubular cells. This reduction in phagocytosis in KIM-1Δmucin mice reduced clearance of apoptotic ...
Snail Mucin (yes, snail slime) What I want to know is- Who was the first person to put this on their face? Why should YOU be interested? Below are some of the claims Ive found for snail mucin through articles, cosmetic brands & ingredient companies. However, I only found one clinical study, and the one…
Among several genes identified by SST-REX cloning, Endomucin is noteworthy, as it encodes a protein that belongs to the sialomucin family. CD34 is the prototypic member of this family and has been widely used as a marker of HSCs and/or progenitor cells and of endothelial cells. The highly conserved amino acid sequence, protein structure, and genomic organization of CD34, endoglycan, and podocalyxin suggest that they are closely related to each other (29). These molecules reportedly are expressed on HSCs and/or progenitor cells and on endothelial cells and are supposed to have redundant functions (29, 30). Although Endomucin exhibits a protein structure highly related to that of these three molecules, its genomic structure is distant, indicating a different phylogeny (19).. Endomucin was originally identified as an endothelium-specific protein (19), but we and others have reported possible involvement of Endomucin in HSC development in the embryo (24, 31). Identification of Endomucin on SST-REX ...
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Gastric cancer (GC) is one of the most common malignancies worldwide. Better knowledge of the changes in gene expression that occur during gastric carcinogenesis may lead to improvements in diagnosis, treatment and prevention. In this study, we screened for genes upregulated in GC by comparing gene expression profiles from microarray and serial analysis of gene expression and identified the HOXA10 gene. The aim of the present study was to investigate the significance of HOXA10 in GC. Immunohistochemical analysis demonstrated that 221 (30%) of 749 GC cases were positive for HOXA10, whereas HOXA10 was scarcely expressed in non-neoplastic gastric mucosa except in the case of intestinal metaplasia. Next, we analyzed the relationship between HOXA10 expression and clinicopathological characteristics. HOXA10 expression showed a significant inverse correlation with the depth of invasion and was observed more frequently in the differentiated type of GC than in the undifferentiated type of GC. HOXA10 ...
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Glycosylation in S2 cells is very similar to that of SF9, Sf21, and High Five cells. The nature of Drosophila N-linked glycosylation is less complex than mammalian glycosylation - it is generally of the paucimannose type and is not trimmed and sialylated.. O-linked glycosylation is similar, although not identical, to that obtained in mammalian cells. However, human glycosylation profiles are difficult to obtain, also glycosylation from CHO and HEK293 differ from human glycosylation. Human O-glycosylation can be divided into N-acetylGalactosamine linked (mucin type), N-acetylGlucosamine lined (O-GlcNAc type) and xylose linked (proteoglycans) families. The most abundant form is the mucin type, while O-GlcNac has only been found for cytoplasmic and nuclear proteins, the proteoglycans are of less interest here.. In CHO cells, O-glycosylation results in terminally sialated mucin type glycans, with a low percentage core-1 structure (T-antigen) reported. In Drosophila S2, cell O-glycosylation is less ...
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JOINT synovial fluid is considered to be a dialysate of blood with added mucin, which consists of hyaluronic acid1. The source of the latter is uncertain, but the belief is current that the synovial membrane is the likely site of formation. Articular cartilage is said to contain no hyaluronic acid, and the sulphated mucopolysaccharide it does contain is not found in appreciable quantity in synovial fluid. Synovial membrane is not lined by an epithelium, but is composed of fine, compact connective tissue of variable cellularity2, the cells being indistinguishable from fibroblasts by electron microscopy3. Claims have been made that the membrane, especially in its superficial part, stains with mucicarmine4-6, and also reacts with the periodic acid - Schiff technique3. Metachromasia, which might indicate the presence of hyaluronic acid in the membrane, has not been unequivocally demonstrated; some claim that the membrane is metachromatic6,7, but others deny it3,5.
Lung diseases that involve dysfunctional development and regeneration of the pseudostratified proximal airway epithelium are a leading cause of morbidity and mortality. A hallmark of the response of the proximal airways to both injury and disease is an increase in the goblet cell lineage with a concomitant increase in mucin production and altered morphology of the pseudostratified epithelium. We show that Foxp1/4 play a central role in restricting secretory cell fate in the proximal airways of the lung by regulation of a novel pathway involving direct repression of the protein disulfide isomerase Agr2. Ectopic expression of Agr2 alone can promote the goblet cell fate in the secretory epithelium of the lung. This result, along with previous findings that Agr2 is essential for intestinal goblet cell development (Zhao et al., 2010), suggests that Agr2 is a central player in defining goblet cell fate and function in the epithelium of multiple tissues including the lung and intestine. Given that ...
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Introduction: The presence of MUC5AC (M1 antigen) and MUC6 have previously been found in ovarian mucinous cyst. We characterized the mucins in the crude mucus and tissue of a mature ovarian tera...
UNC School of Medicine researchers have provided the first quantitative evidence that mucins -- the protein framework of mucus -- are significantly increased in cystic fibrosis patients and play a major role in failing lung function.
They analysed gastrointestinal mucins initially because the oligosaccharides were similar to those of cell membranes but mucins ... In addition, mucins were an important part of cellular defences so of interest in their own right. They were able to define the ... Hounsell, E F; Feizi, T (1982). "Gastrointestinal mucins. Structures and antigenicities of their carbohydrate chains in health ... Biochemical Society Transactions 12 (4) 591-596 E F Hounsell and T Feizi (1982) Gastrointestinal mucins - structures and ...
The major mucins secreted - MUC5AC, and MUC5B are large polymers that give the mucus its rheologic, or viscoelastic properties ... MUC5AC is the main gel-forming mucin secreted by goblet cells, in the form of threads, and thin sheets. MUC5B is a polymeric ... Mucins, a kind of polyelectrolyte proteoglycans, are the main component of mucus, which provides the polyelectrolyte effect in ... For instance, the charged residue on mucin is protonated at a normal pH value of the stomach, approximately pH 2. In this case ...
Die Mucin-Polysaccharide der Eiweißdrüse. 1. Mitteil.: Die Identität von Galaktogen und Sinistrin. Hoppe-Seyler's Zeitschrift ...
... stains acid mucins to yellow color. It also stains mast cell granules brown. It can be used with live cells. ...
The main component of mucus is a glycoprotein called mucin and the type secreted varies according to the region involved. Mucin ... It covers the cheeks, inner surfaces of the lips, and floor of the mouth, and the mucin produced is highly protective against ... Frenkel, Erica Shapiro; Ribbeck, Katharina (2014-10-24). "Salivary Mucins Protect Surfaces from Colonization by Cariogenic ...
Hanisch FG (February 2001). "O-glycosylation of the mucin type". Biological Chemistry. 382 (2): 143-9. doi:10.1515/BC.2001.022 ...
The target might be Mucin 5AC. The antibody was discovered by scientists at Neogenix Oncology using tumor antigens that had ...
They secrete neutral mucin that protects the esophagus from acidic gastric juices. They are simple tubular or branched tubular ... They secrete acid mucin for lubrication.[citation needed] Esophageal cardiac glands- mucous glands located near the cardiac ...
Acinar adenocarcinoma with intraluminal blue mucin. In uncertain cases, a diagnosis of malignancy can be discarded by ... Prominent nucleoli Intraluminal eosinophilic secretion Intraluminal blue mucin Adenocarcinoma with two mitoses in reactive ...
These include inflammatory cytokines, carbonic anyhydrase, E-cadherin and mucins; however, their direct implications in LPR are ...
Occurrence, partial purification, and action on linkages in submaxillary mucins". Biochemistry. 8 (5): 2034-43. doi:10.1021/ ...
Mucin-like protein 1 is a protein that in humans is encoded by the MUCL1 gene. GRCh38: Ensembl release 89: ENSG00000172551 - ... Hubé F, Mutawe M, Leygue E, Myal Y (2005). "Human small breast epithelial mucin: the promise of a new breast tumor biomarker". ... "Entrez Gene: SBEM small breast epithelial mucin". Dias Neto E, Correa RG, Verjovski-Almeida S, et al. (2000). "Shotgun ... 2006). "Identification of an octamer-binding site controlling the activity of the small breast epithelial mucin gene promoter ...
Mucins are large glycoproteins that give the mucus its gel-like properties. As mucins are lost during routine histology ... Below the mucin granules, surface mucous cells have a Golgi apparatus, the nucleus, and small amounts of rough endoplasmic ... Surface mucous cells have large quantities of mucin granules in their apical (top) surface and project short microvilli into ... These are shorter than their surface counterpart and contain lesser quantities of mucin granules in their apical surface. The ...
Mucin production diminishes in the presence of inflammation. Only when some inflammatory condition co-occurs with E. coli ...
Microscopically, mucoceles appears as granulation tissue surrounding mucin. Since inflammation occurs concurrently, neutrophils ...
In 1950 it was used by Steedman as a selective dye for mucins. While the popularity of Alcian blue expanded exponentially, the ... GB 586340 GB 587636 Steedman H. F. (1950). "Alcian blue 8GS: a new stain for mucin". Q. J. Microsc. Sci. 91 (4): 477-479. PMID ... Green G Luxol fast blue Isothiouronium Alcian Yellow Imperial Chemical Industries Glycosaminoglycan Proteoglycan Mucin ...
Gipson IK, Hori Y, Argüeso P (April 2004). "Character of ocular surface mucins and their alteration in dry eye disease". Ocul ...
The cells produce mucin, and exhibit an epithelial morphology. BxPC-3 cells lack a KRAS mutation, though it is commonly found ...
The layer of mucin allows tears to glide evenly across the eye's surface. The crypts of Henle are named after German anatomist ... They are responsible for secreting mucin, a proteinous substance that makes up the inner layer of tears. It coats the cornea to ... They also are responsible for secreting mucin into tears. Essay on "Dry Eye" v t e. ...
Central necrosis with eosinophilic, granular features may be also present, as well as mucin. The heavy aggregates of mucin ... Such clefts are caused by shrinkage of mucin during tissue fixation and staining. ...
... secrete the gel-forming mucin protein MUC5AC. They might secrete proteins such as lactoferrin, lysozyme, ... "Aquaporin pathways and mucin secretion of olfactory glands might protect the olfactory mucosa.", Chemical Senses, 37 (1): 35-46 ...
"Helicobacter pylori moves through mucus by reducing mucin viscoelasticity". Proceedings of the National Academy of Sciences. ...
"Ueber die Bildung von Elweiss und Mucin durch Tuberkelbacillen". Klinische Wochenschrift (Clinical Weekly). 28: 1-8 Berlin, ...
Mucin Kresge, Nicole, Robert D. Simoni, and Robert L. Hill. "Hepatic Carbohydrate Binding Proteins and Glycoprotein Catabolism ...
This research furthered mucin knowledge regarding its structure and properties. With the Bertozzi lab, Kramer explored design ... Chem Commun (Camb) 2013 Jun 7;49(45):5144-6 Kramer JR, Onoa B, Bustamante C, Bertozzi CR, Chemically tunable mucin chimeras ... Kramer designed a synthetic route to produce mucin glycodomain constructs using NCA polymerization. ...
These include acinar cells, which secrete bicarbonate solution and mucin. → Main article: Exocrine pancreas. Exocrine pancreas ...
Kitazaki T, Soda H, Doi S, Nakano H, Nakamura Y, Kohno S (2005). "Gefitinib inhibits MUC5AC synthesis in mucin-secreting non- ... Kitazaki T, Fukuda M, Soda H, Kohno S (2005). "Novel effects of gefitinib on mucin production in bronchioloalveolar carcinoma; ...
de Galantha, E. (1936). "A new stain for connective tissue, mucin, and allied substances". Am. J. Clin. Path. 6, 196-197. de ... de Galantha, E. (1936). "A new stain for connective tissue, mucin, and allied substances". Am. J. Clin. Path. 6, 196-197. Cain ...
Mucins are negatively charged, which allows them to interact with water and prevent it from evaporating. This is important in ... Mucins are a group of heavily O-glycosylated proteins that line the gastrointestinal and respiratory tracts to protect these ... Changes in mucins are important in numerous diseases, including cancer and inflammatory bowel disease. Absence of O-glycans on ... mucin proteins changes their 3D shape dramatically and often prevents correct function. Addition of N-acetylglucosamine (O- ...
CS1 maint: discouraged parameter (link) Ward AM (July 1971). "Glandular metaplasia and mucin production in transitional cell ...
Overexpression of the mucin proteins, especially MUC1, is associated with many types of cancer. Although some mucins are ... Mucin genes encode mucin monomers that are synthesized as rod-shapedapomucin cores that are post-translationally modified by ... Mucins at the US National Library of Medicine Medical Subject Headings (MeSH) "Mucin" at Dorlands Medical Dictionary. ... N-linked oligosaccharides are also found on mucins, but in less abundance than O-linked sugars. Mucins have been found to have ...
Mucins belong to an ever increasing family of O-glycoproteins. Secreted mucins are the main component of mucus protecting the ... Moreover, we will discuss the recent advances about the biology of both secreted and membrane-bound mucins and their key roles ... In this review, we will focus on the altered expression pattern of mucins in pancreatic cancer, from the early neoplastic ... Finally, we will discuss exciting opportunities that mucins offer as potential therapeutic targets in pancreatic cancer. ...
... mucins in the small intestine of the CF mice compared with wild-type mice. Mucins from the large intestine of both wild-type ... Intestinal mucins from cystic fibrosis mice show increased fucosylation due to an induced Fucalpha1-2 glycosyltransferase.. ... In contrast, the insoluble and soluble mucins of the small intestine in CF mice revealed a large increase in fucose, whereas ... The amino acid composition was typical of that for mucins and showed increased amounts of the insoluble (2.5-fold increase) and ...
Mucin also lubricates the tissues and teeth in the mouth and protects them against pathogens and... ... A critical function of mucin in saliva is to protect the mouth from drying out. ... There are two forms of salivary mucins: high or low molecular weight mucins. Low molecular weight mucin is found more often in ... Mucin is a glycosated protein, which means that it is coated with carbohydrates. This coating allows mucin to hold on to water ...
Mouse monoclonal Mucin 5AC antibody [CLH2] validated for WB, IP, IHC, Flow Cyt, I-ELISA, ICC/IF and tested in Human. Immunogen ... Mucins are high molecular weight glycoproteins with 80% carbohydrates and 20% core protein. Gastric Mucin 5AC antigen is found ... Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric ... mucin in the adult colon or due to changes in the glycosylation pattern of mucin, which expose the hidden Mucin 5AC antigen. ...
The mucin-containing mucus layer coating the GI epithelium is the front line of innate host defense. Mucins are likely to be ... In addition, we describe the current knowledge on the role of mucins in intestinal innate defense. It is the aim of this review ... The concept of the mucus layer functioning as a dynamic protective barrier is suggested by studies showing changes in mucins in ... to provide the readers with an update on goblet cell biology and current understanding on the role of mucins in host defense in ...
... Yan, Hongji KTH, School of Engineering Sciences in Chemistry, ... Mucins are high molar mass glycoproteins that assume an extended conformation and can assemble into mucus hydrogels that ... We aimed to recreate and control the process of compaction for mucins, the first step toward a better understanding of the ... The change of structure of mucins from extended molecules to nano-sized particles in the presence of glycerol translated into ...
Using this method, heterogeneous glycosylation of mucins and mucin-type molecules isolated by SDS-AgPAGE and SDS-UAgPAGE was ... glycomics, mucin, o-linked glycosylation, software Subject categories. Medical Biotechnology (with a focus on Cell Biology ( ... Analysis of mucosal mucins separated by SDS-urea agarose polyacrylamide composite gel electrophoresis. Journal article ... Efficient separation of mucins (200 kDa-2 MDa) was demonstrated using gradient SDS agarose/polyacrylamide composite gel ...
Monoclonal Anti-Mucin Gastric antibody produced in mouse for your research needs. Find product specific information including ... mucin from human ovarian cyst fluid. General description Mucin has a netlike, branched structure and is a secreted protein. It ... The antibody recognizes the mucin epitope g located in the peptide core of gastric mucin (,1,000 kDa). This epitope is ... Monoclonal Anti-Mucin Gastric antibody produced in mouse clone 45M1, ascites fluid ...
GPI-Linked Surface Mucin) Ab-1, Mouse 1mL; 200µg/mL; Unlabeled; Purified with ... Thermo Scientific Lab Vision CD24 (GPI-Linked Surface Mucin) Ab-1, Mouse Monoclonal Antibody ... Thermo Scientific Lab Vision CD24 (GPI-Linked Surface Mucin) Ab-1, Mouse Monoclonal Antibody ...
Mouse Monoclonal Anti-Mucin 5AC Antibody (SPM297) [DyLight 405]. Validated: WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP. Tested ... Home » Mucin 5AC » Mucin 5AC Antibodies » Mucin 5AC Antibody (SPM297) [DyLight 405] ... Mucin 5AC Antibody (SPM297) [DyLight 405] Summary. Immunogen. M1 mucin preparation from the fluid of an ovarian mucinous cyst ... Reviews for Mucin 5AC Antibody (NBP2-34758V) (0) There are no reviews for Mucin 5AC Antibody (NBP2-34758V). By submitting a ...
Mouse Monoclonal Anti-Mucin 5AC Antibody (45M1) - Azide and BSA Free. Validated: WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP, ... Home » Mucin 5AC » Mucin 5AC Antibodies » Mucin 5AC Antibody (45M1) - Azide and BSA Free ... Mucin 5AC Antibody (45M1) - Azide and BSA Free Summary. Immunogen. M1 mucin preparation from the fluid of an ovarian mucinous ... Blogs on Mucin 5AC. There are no specific blogs for Mucin 5AC, but you can read our latest blog posts. ...
Expression of Mucin-associated Sulfo-Le^a Carbohydrate Epitopes on Human Colon Carcinoma Cells * * TSUIJI Hitomi ... Inhibition of mucin glycosylation by aryl-N-acetyl-a-galactosaminidases in human colon cancer cells. KUAN S. F. ... Sialylation and sulfation of the carbohydrate chains in respiratory mucins from a patient with cystic fibrosis LO-GUIDICE J.-M ... Sulfated Lewis^x determinants-a major structural motif in glycans from LS174T HM7 human colon carcinoma mucin CAPON C. ...
Identification of trans Protein QTL for Secreted Airway Mucins in Mice and a Causal Role for Bpifb1.. Donoghue LJ1,2, Livraghi- ... The mucin proteins MUC5AC and MUC5B are the major glycoprotein components of mucus and have critical roles in airway defense. ... To identify genes that underlie variation in airway mucin levels, we performed genetic analyses in founder strains and ... Identification of trans Protein QTL for Secreted Airway Mucins in Mice and a Causal Role for Bpifb1 ...
"Entrez Gene: MUC20 mucin 20, cell surface associated". Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: ... Mucin-20 is a protein that in humans is encoded by the MUC20 gene. ENSG00000281630, ENSG00000277753, ENSG00000275501, ... a novel mucin protein, up-regulated in injured kidney". J Biol Chem. 279 (3): 1968-79. doi:10.1074/jbc.M304558200. PMID ...
The vast majority of mucin glycosylation is constituted by O... ... MUC Mucins (MUC) are high-molecular-weight glycoproteins ... There are two main classes of mucins: membrane-bound mucins, contributing to the composition of the cellular glycocalyx, and ... of the mucin molecular mass is composed of carbohydrates. The primary functions of mucins are to hydrate, protect, and ... Mucins share a common structural feature which is a tandem repeatdomain comprising a variable number of tandem repeats (VNTRs) ...
Mucin (sv); mucin (nn); מוצין (he); Mucin (nl); Mucin (sh); Муцин (uk); Mucyny (pl); 뮤신 (ko); Mucina (gl); ميوسين (ar); Mucin ( ... mucina (it); Mucine (fr); Mutsiinid (et); Mucin (sr-el); Муцин (sr-ec); Մուցիններ (hy); mucina (ca); mucin (en); Mucine (de); ... Mucin (de); Mutsiin (et); mucins (en); Mucyna (pl); 黏蛋白 (zh); Mucini (sr) ... File nella categoria "Mucins". Questa categoria contiene 14 file, indicati di seguito, su un totale di 14. ...
Mucins in gastrointestinal cancers.. Turner MS1, McKolanis JR, Ramanathan RK, Whitcomb DC, Finn OJ. ... The mucin family has been under study by molecular biologists, biochemists, pathologists and immunologists interested in cancer ... For example, specific knowledge of cancer-associated changes in the expression and glycosylation of various mucins, which can ... Similarly, many more opportunities exist for using mucin-based therapies than are currently being considered in the clinic. ...
... to weigh in on the benefits of snail mucin for skin and polled them on their recommendations for the best snail mucin products ... Snail mucin was a gateway into K-beauty for our beauty writer Rio Viera-Newton, whose first Google doc included a Cosrx snail- ... A fixture among Rios skin-care stories is this Cosrx snail-mucin essence, which she says is a cheaper alternative to SK-II. ... Most of the snail mucin used for skin care involves the Cryptomphalus aspersa species, a.k.a. the common garden snail. Their ...
Gel-Forming Mucins in Nonvertebrate Chordates.. We identified mucins and mucin-related proteins in a variety of vertebrates as ... Other proteins with shorter mucin domains, like IgA and several receptors, are not called mucins. The mucin domains often ... "NIDO-AMOP-VWD mucins" (or "MUC4′") for these categories of mucins that previously were listed under transmembrane mucins. ... The Muc4 mucins form a group distinct from the other VWD-containing mucins, because they do not contain cysteines and have NIDO ...
Mucin-20 (MUC20) was identifiied as a gene that is up-regulated in the renal tissues of patients with immunoglobulin A ... Molecular cloning, genomic structure, and expression analysis of MUC20, a novel mucin protein, up-regulated in injured kidney. ...
... or non mucin-secreting cells [PMID: 15190477]. There are two main mucin subfamilies: secreted mucins or gel-forming mucins ( ... Mucin-2 (MUC2) belongs to the mucin family. They are high molecular weight proteins that are components of mucus and are ... MUC genes: mucin or not mucin? That is the question].. Med Sci (Paris) 20 569-74 2004 ... MUC genes: mucin or not mucin? That is the question].. Med Sci (Paris) 20 569-74 2004 ...
UNC School of Medicine researchers have provided the first quantitative evidence that mucins -- the protein framework of mucus ... This is where mucins come into play. Mucins give mucus its gel-like thickness and elasticity. "Without mucins, mucus would have ... And so it looked as if there were fewer mucins. "But by using more accurate methods, we clearly saw the increase of mucins. In ... increased-mucins-pinned-to-worsening-cystic-fibrosis-symptoms More in Medicine & Health. * Newborn babies who suffered stroke ...
JOINT synovial fluid is considered to be a dialysate of blood with added mucin, which consists of hyaluronic acid1. The source ... The Stainable Mucin of Joint Tissues and Synovial Fluid. *B. F. MARTIN1. & ... JOINT synovial fluid is considered to be a dialysate of blood with added mucin, which consists of hyaluronic acid1. The source ... MARTIN, B., SHAW, N. The Stainable Mucin of Joint Tissues and Synovial Fluid. Nature 190, 835-836 (1961). https://doi.org/ ...
... Hans-Peter Hauber,1,2 Susan C Foley,1 and Qutayba Hamid1 ... Hans-Peter Hauber, Susan C Foley, and Qutayba Hamid, "Mucin Overproduction in Chronic Inflammatory Lung Disease," Canadian ...
... up-regulate mucin expression and secretion. Recently, downstream signaling causing mucin MUC2 expression induced by Pseudomonas ... Epidermal growth factor system regulates mucin production in airways. Kiyoshi Takeyama, Karim Dabbagh, Heung-Man Lee, Carlos ... Epidermal growth factor system regulates mucin production in airways. Kiyoshi Takeyama, Karim Dabbagh, Heung-Man Lee, Carlos ... We investigated the role of EGF-R and its ligands in mucin production in airway epithelium by using NCI-H292 cells (23) and by ...
... Peter L. Devine,1 Rachel J. Quin,1 Paul W. Shield,2 Yew Wah Liew,3 ... "Monoclonal Antibody Recognizing a Core Epitope on Mucin," Disease Markers, vol. 14, no. 2, pp. 99-112, 1998. https://doi.org/ ...
Frequently in tumors of the pancreas there are changes in the structure of mucin carbohydrates and/or levels of apomucin types ... Mucins are large glycoproteins that form a protective layer along the lumens of the organs of the gastrointestinal and ... Mucins in the diagnosis and therapy of pancreatic cancer Curr Pharm Des. 2000 Dec;6(18):1881-96. doi: 10.2174/1381612003398609 ... The carbohydrates of mucins on the surface of tumor cells have been reported to inhibit cells of the immune system. These ...
In, Mucins: Methods and Protocols expert researchers in the field detail many of the ... Epithelial mucins are large complex cell surface and secreted glycoproteins produced by mucosal epithelial cells. ... Mucin Methods: Genes Encoding Mucins and Their Genetic Variation with a Focus on Gel-Forming Mucins ... Authoritative and practical, Mucins: Methods and Protocols is designed to be a useful resource for those entering the mucin ...
Repeat Mucin Ball Former Non-repeat Mucin Ball Former Arm/Group Description: Subjects that were classified as be... Subjects ... Repeat Mucin Ball Former Non-repeat Mucin Ball Former Arm/Group Description: Subjects that were classified as be... Subjects ... Subjects that were classified as being repeat Mucin Ball former. Subjects that were classified as Non-repeat Mucin Ball Former ... Szczotka-Flynn LB, Jiang Y, Stiegemeier MJ, Mutti DO, Walline J, Wilson T, Debanne S; Mucin Ball Study Group. Mucin Balls ...
In this study, we liberated and purified O-linked glycans from porcine gastric mucin and assessed their efficacy in shaping the ... Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes ... We found that porcine mucin glycans (PMGs) and HMOs enrich for taxonomically similar resident microbes. We demonstrate that ... 3c) harbor very few mucin-degrading CGCs (Fig. S7).. Fig. 5: Prevalent gut commensals harbor high numbers of mucin-degrading ...
The O-glycan structures present in mucin are diverse and complex, consisting predominantly of core 1-4 mucin-type O-glycans ... Mucin glycan foraging in the human gut microbiome Front Genet. 2015 Mar 19;6:81. doi: 10.3389/fgene.2015.00081. eCollection ... Due to their proximity to the immune system, mucin-degrading bacteria are in a prime location to influence the host response. ... The ability to metabolize these mucin O-linked oligosaccharides is likely to be a key factor in determining which bacterial ...
The purpose of this study is to determine if the formation of mucin balls is a marker for protection from inflammatory events ... The percentage of Subjects that experienced Corneal Inflammatory Events within their Mucin Ball classification. ... The percentage of Subjects that experienced Corneal Inflammatory Events within their Mucin Ball classification. ... to the cornea and if mucin balls play a role in protection during extended contact lens wear. ...
The aim of this group is to expand the knowledge of mucus and mucins in relation to diseases of the intestine and respiratory ... This group, headed by Prof Gunnar C. Hansson, is a central part of the Mucin Biology Groups which now consists of a number of ... The aim of this group is to expand the knowledge of mucus and mucins in relation to diseases of the intestine and respiratory ... For detailed and updated descriptions of the groups research as well as more information on all the Mucin Biology Groups, ...
Mucin-4. Mucin-4, MUC-4 (Ascites sialoglycoprotein, ASGP) (Pancreatic adenocarcinoma mucin) (Testis mucin) (Tracheobronchial ... mucin) [Cleaved into: Mucin-4 alpha chain (Ascites sialoglycoprotein 1, ASGP-1) ; Mucin-4 beta chain (Ascites sialoglycoprotein ... Mucin-4Imported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p>,a href="/ ... tr,A0A0G2JRU8,A0A0G2JRU8_HUMAN Mucin-4 OS=Homo sapiens OX=9606 GN=MUC4 PE=1 SV=1 ...
Mucin-4. Mucin-4, MUC-4 (Ascites sialoglycoprotein, ASGP) (Pancreatic adenocarcinoma mucin) (Testis mucin) [Cleaved into: Mucin ... Mucin-4Imported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p>,a href="/ ... R-MMU-913709 O-linked glycosylation of mucins. R-MMU-977068 Termination of O-glycan biosynthesis. ... R-MMU-913709 O-linked glycosylation of mucins. R-MMU-977068 Termination of O-glycan biosynthesis. ...
  • MUC5AC (gastric mucin) belongs to the gel-forming mucin family of glycoproteins, the major components of the protective mucus layer on the mucosal surfaces of epithelial tissues. (novusbio.com)
  • Mucins are high-molecular weight glycoproteins (0.25-20 MDa) containing one or more domains that are heavily O-glycosylated. (manchester.ac.uk)
  • Mucins are linear O-glycosylated glycoproteins involved in inflammation, cell adhesion, and tumorigenesis. (elsevier.com)
  • There are currently no images for Mucin 5AC Antibody (NBP2-34758V). (novusbio.com)
  • Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. (novusbio.com)
  • Immunohistochemistry-Paraffin: Mucin 5AC Antibody (45M1) - Azide and BSA Free [NBP2-32732] - Formalin-fixed, paraffin-embedded Rat Stomach stained with MUC5AC Monoclonal Antibody (45M1). (novusbio.com)
  • Immunohistochemistry-Paraffin: Mucin 5AC Antibody (45M1) [Azide Free] [NBP2-32732] - Human stomach. (novusbio.com)
  • Mouse IgG monoclonal antibody for Mucin Gastric, mucin 5AC, oligomeric mucus/gel-forming (MUC5AC) detection. (bosterbio.com)
  • Mucins (/ˈmjuːsɪn/) are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by epithelial tissues in most animals. (wikipedia.org)
  • Overexpression of the mucin proteins, especially MUC1, is associated with many types of cancer. (wikipedia.org)
  • In the mouth, mucins can also recruit anti-microbial proteins such as statherins and histatine 1, which further reduces risk of infection. (wikipedia.org)
  • Mucins are secreted as massive aggregates of proteins with molecular masses of roughly 1 to 10 million Da. (wikipedia.org)
  • Mucins ( / ˈ m juː s ɪ n / ) are a family of high molecular weight , heavily glycosylated proteins ( glycoconjugates ) produced by epithelial tissues in most animals . (wikipedia.org)
  • Skin mucin essence is said to be rich with ingredients that aid in skin health, including peptides, proteins, antioxidants, and hyaluronic acid. (themixseattle.com)
  • Mucins are linear, heavily O-glycosylated proteins with physiological roles that include cell signaling, cell adhesion, inflammation, immune response and tumorgenesis. (elsevier.com)
  • Mucin genes encode mucin monomers that are synthesized as rod-shapedapomucin cores that are post-translationally modified by exceptionally abundant glycosylation. (wikipedia.org)
  • Using this method, heterogeneous glycosylation of mucins and mucin-type molecules isolated by SDS-AgPAGE and SDS-UAgPAGE was shown to consist of more than 80 different components in a single band, and in the extreme cases, up to 300-500 components (MUC5B/AC from saliva and sputum and). (gu.se)
  • The major secreted airway mucins are MUC5AC and MUC5B, while MUC2 is secreted mostly in the intestine but also in the airway. (wikipedia.org)
  • To know the role played by the MUC2 and MUC4 mucins genes in cancers of the upper aerodigestve tract. (alliedacademies.org)
  • For the study of mucins "MUC2 et MUC4" the molecular biology technique used was immunohistochemistry on pieces of laryngeal biopsies embedded in parafn. (alliedacademies.org)
  • On immunohistochemistry, the mucins MUC2 and MUC4 were present in the normal mucosa of the upper aero-digestve tract and gradually lost their expression from the metaplastc mucosa to the tumor mucosa (P=0.03). (alliedacademies.org)
  • These mucins "MUC2 and MUC4" were absent on tumor mucosa in both smoking and non-smoking patents with a statstcally signifcant di?erence (P=0.01). (alliedacademies.org)
  • This work evaluates the expression in lung cancer of the most well characterized mucin genes (MUC1, MUC2, MUC3) and of the recently described MUC4 in lung tissues, to check a correlation between the expression of any particular gene and this tumor. (elsevier.com)
  • This MAb recognizes the peptide core of gastric mucin M1 (>1,000kDa) (recently identified as Mucin 5AC). (novusbio.com)
  • Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins. (novusbio.com)
  • Two membrane mucins, MUC1 and MUC4 have been extensively studied in relation to their pathological implication in the disease process. (wikipedia.org)
  • We have shown that MG1 is actually a mixture of mucin gene products, containing MUC4, MUC5B and a novel salivary mucin (NSM), whereas MG2 is a single gene product encoded in the MUC7 gene. (grantome.com)
  • determine the structural organization and sequence of mucin gene products comprising MG1 by characterization of a novel salivary mucin (NSM) and elucidating the C-terminal sequence of MUC4. (grantome.com)
  • 2. investigate the biosynthesis and assembly of MUC4, MUC5B and NSM using mucin constructs expressed in COS-7 cells. (grantome.com)
  • As expected, the highest reactivity for MUC1 and MUC4 expression was observed mainly in the adenocarcinoma histotype which is mucin secreting. (elsevier.com)
  • These findings represent a contribution to the study of mucin gene pattern in lung cancer, and, in particular, indicate that MUC4, in association with the MUC1 gene, seems to be strongly expressed in this neoplastic disease. (elsevier.com)
  • A recently identified human mucin gene is MUC21, previously known as chromosome 6 open reading frame 205. (wikipedia.org)
  • Eosinophils were isolated from the peripheral blood of allergic patients, and their effects on MUC5AC mucin gene and protein synthesis were assessed using in situ hybridization and ELISAs. (jimmunol.org)
  • EGF, TGF-α) resulted in MUC5AC mucin gene and protein synthesis in human airway epithelial (NCI-H292) cells in vitro, and selective inhibitors of EGFR tyrosine kinase phosphorylation prevented ligand-induced mucin synthesis in vitro and prevented allergen-induced goblet cell metaplasia in pathogen-free rats ( 13 ). (jimmunol.org)
  • MUC5B, the predominate mucin in the mouth and female genital tract, has been shown to significantly reduce attachment and biofilm formation of Streptococcus mutans, a bacteria with the potential to form cavities. (wikipedia.org)
  • Similar effects of MUC5B and other mucins have been demonstrated with other pathogens, such as Candida albicans, Helicobacter pylori, and even HIV. (wikipedia.org)
  • Bovine Submaxillary Gland and human MUC5B mucins were used as proof of concept and were successfully detected and quantified at different stages of purification. (manchester.ac.uk)
  • Intestinal mucins from cystic fibrosis mice show increased fucosylation due to an induced Fucalpha1-2 glycosyltransferase. (gu.se)
  • Comparison of structural characteristics of major membrane-bound mucins from porcine milk and intestinal brush borders. (sdstate.edu)
  • Liu, C., "Comparison of structural characteristics of major membrane-bound mucins from porcine milk and intestinal brush borders. (sdstate.edu)
  • The purpose of this study was to determine the distribution and diffusion of sodium taurocholate-phospholipid micelles with mucin in order to provide the foundation for understanding the transport of ingested fat and poorly water-soluble drugs through the intestinal mucous layer. (umn.edu)
  • Upon stimulation, MARCKS (myristylated alanine-rich C kinase substrate) protein coordinates the secretion of mucin from mucin-filled vesicles within the specialized epithelial cells. (wikipedia.org)
  • Snail mucin, or snail secretion filtrate, is that gooey, secretion that snails leave on their trail. (themixseattle.com)
  • SNAP23 Is Selectively Expressed in Airway Secretory Cells and Mediates Baseline and Stimulated Mucin Secretion. (escholarship.org)
  • Airway mucin secretion is important pathophysiologically and as a model of polarized epithelial regulated exocytosis. (escholarship.org)
  • Heterozygous SNAP23 deletant mutant mice show spontaneous accumulation of intracellular mucin, indicating a defect in baseline secretion. (escholarship.org)
  • 5. examine MG1 and MG2 levels in salivary secretions and whole saliva and determine secretion profiles using mucin-specific capture ELISAs. (grantome.com)
  • Made with all-natural 96% snail secretion filtrate, Advanced Snail 96 Mucin Power Essence is an essence that transforms the skin. (cupidrop.com)
  • Made with all-natural domestic 96% snail secretion filtrate, the Advanced Snail 96 Mucin Power Essence is a Real Power Essence that transform the skin while keeping in the moisture and encouraging the real power of skin in a healthy way. (ibuycrapsoyoudonthaveto.com)
  • Bovine submaxillary mucin coatings Verotoxin-producing Escherichia coli Marin F, Luquet G, Marie B, Medakovic D (2007). (wikipedia.org)
  • We have used AFM based single-molecule forced unbinding under near physiological conditions to investigate the self-interactions between porcine submaxillary mucin (PSM) as well as between PSM analogs possessing various carbohydrates including the T- and Tn-antigen. (elsevier.com)
  • PC/TC micellar solutions were dialyzed against fixed and variable concentrations of bovine submaxillary mucin after which the concentration of PC and NaTC was measured by an assay for total phosphorus and by HPLC, respectively. (umn.edu)
  • In this work, heterotypic interactions of model mucins that possess the Tn (GalNA-cαThr/Ser) and T (Galβ1-3GalNAcαThr/Ser) cancer antigens derived from porcine submaxillary mucin (PSM) were studied using atomic force microscopy. (elsevier.com)
  • These different pathways of M1 antigen degradation suggest the occurrence of different epitopes located in separate regions of the mucin molecules. (mysciencework.com)
  • in particular, the epithelial mucin surface antigen 1 is overexpressed in adenocarcinomas in an aberrant form [ 2 - 5 ]. (biomedcentral.com)
  • All mucin samples investigated showed self-interaction, but the tendency was greatest for PSM displaying only the Tn-antigen (Tn-PSM) or a mixture of Tn-, T-antigen, and the trisaccharide Fucα1,2Galβ1,3GalNAc (Tri-PSM). (elsevier.com)
  • This suggests common carbohydrate epitope interactions for the Tn cancer antigen with the above mucin analogs, a finding that may be important to the role of the Tn antigen in cancer cells. (elsevier.com)
  • Along with Hyaluronic acid, snail mucin essence also contains copper, antimicrobial peptides, and glycoprotein that protects and soothes irritated skin. (themixseattle.com)
  • Eosinophil recruitment and mucus hypersecretion are characteristic of asthmatic airway inflammation, but eosinophils have not been shown to induce mucin production. (jimmunol.org)
  • Because an epidermal growth factor receptor (EGFR) cascade induces MUC5AC mucin in airways, and because EGFR is up-regulated in asthmatic airways, we examined the effect of eosinophils on MUC5AC mucin production in NCI-H292 cells (a human airway epithelial cell line that produces mucins). (jimmunol.org)
  • These results show that activated eosinophils induce mucin synthesis in human airway epithelial cells via EGFR activation, and they implicate TGF-α produced by eosinophils and epithelial cells in the EGFR activation that results in mucin production in human airway epithelium. (jimmunol.org)
  • Parameter f* varied in the range 27 to 50 pN at force loading rates of about 2 nN/s among the various mucins. (elsevier.com)
  • Low molecular weight mucin is found more often in saliva than high molecular weight mucin. (reference.com)
  • It is also abundant in people who are resistant to cavities, and can even change high molecular weight mucin into the lower molecular form. (reference.com)
  • Salivary secretions contain two distinct mucins, a high molecular weight mucin named MG1 and a low molecular weight mucin named MG2. (grantome.com)
  • Emerging studies have elaborated on the role of mucins in diverse biological functions, including enhanced tumorigenicity, invasiveness, metastasis and drug resistance through their characteristic O-linked and N-linked oligosaccharides (glycans), extended structures and unique domains. (elsevier.com)
  • The data are consistent with the truncated Tn and T glycans enhancing self-interaction of the mucins. (elsevier.com)
  • Mucin 1 (MUC1) is a highly glycosylated transmembrane protein expressed on the apical surface of epithelial cells and is aberrantly overexpressed in the majority of malignant epithelial tumors, including breast, lung, ovarian, prostate and pancreatic cancer and several types of malignant hematological tumor ( 1 ). (spandidos-publications.com)
  • Therefore, mucin 1 is a specific and appropriate target as an immunotherapy for EOC, whereby the immune system is stimulated to target and destroy tumor cells. (biomedcentral.com)
  • N-linked oligosaccharides are also found on mucins, but in less abundance than O-linked sugars. (wikipedia.org)
  • Hence, the implementation of urea makes the technique applicable for high throughput isolation and screening of mucin oligosaccharides by LC-MS after releasing the oligosaccharides from isolated, blotted mucin subpopulations. (gu.se)
  • Metabolomic software was also used to show that the migration of mucin isoforms within the gel is due to heterogeneous size distribution of the oligosaccharides, with the slower migrating bands enriched in high-molecular-weight oligosaccharides. (gu.se)
  • Cancer-associated mucins often possess increased expression of the T (Galβ1,3GalNAcαThr/Ser) and Tn (GalNAcαThr/Ser) cancer antigens, which are diagnostic markers for several cancers, including colon cancer. (elsevier.com)
  • These carbohydrate cancer antigens may, thus, play an active role in the disease by constitutively activating mucin and mucin-type receptors by self-association on cells. (elsevier.com)
  • Salivary mucin also plays a role in the protection of tooth enamel. (reference.com)
  • There are two forms of salivary mucins: high or low molecular weight mucins. (reference.com)
  • Salivary mucins are also crucial to the balance of calcium in the mucous membranes. (reference.com)
  • The dense "sugar coating" of mucins gives them considerable water-holding capacity and also makes them resistant to proteolysis, which may be important in maintaining mucosal barriers. (wikipedia.org)
  • What's Snail Mucin Essence? (themixseattle.com)
  • From being mere garden dwellers, snails have become an abundant source of one of the most raved about ingredients in skincare products - snail mucin essence. (themixseattle.com)
  • Cosrx, a renowned Korean skincare brand , distributes a skincare serum infused with a whopping 96% snail mucin essence. (themixseattle.com)
  • Snail mucin essence help in improving over-all skin health, making it feel smoother and look younger. (themixseattle.com)
  • Continued use of skincare products rich in snail mucin essence aids in fading acne scars, rosacea, burns, razor bumps, and flat warts. (themixseattle.com)
  • Rich in Hyaluronic acid, snail mucin essence has the ability to retain the skin's natural moisture without making it feel oily. (themixseattle.com)
  • With its ability to help treat a variety of skin conditions, from slowing down the signs of aging to helping boost the skin's healing process, there's no wonder why snail mucin essence is considered a trending powerhouse ingredient in skincare. (themixseattle.com)
  • In the morning, apply Cosrx Snail Mucin Essence, and in the evenings apply Cosrx Snail All-in-One Cream . (glamourflare.com)
  • Mucin also lubricates the tissues and teeth in the mouth and protects them against pathogens and toxins. (reference.com)
  • Mucins protect oral surfaces against desiccation, form a selectively permeable diffusion barrier between underlying surfaces and the external environment, lubricate hard and soft tissues to minimize mechanical injury, facilitate speech and swallowing and provide a physical barrier to protect against colonization of potentially harmful microbes and viruses. (grantome.com)
  • Overall, this information should help to delineate the intricacies of pancreatic cancer by exploring the family of mucins, which, through various mechanisms in both tumour cells and the microenvironment, worsen disease outcome. (elsevier.com)
  • Characterization of the binding properties of mucins with truncated carbohydrate side chains could thus prove relevant for understanding their role in cancer mechanisms such as metastasis and recognition by the immune system. (elsevier.com)
  • In contrast, acute stimulated release of intracellular mucin from mutant mice is impaired whether measured by a static imaging assay 5 minutes after exposure to the secretagogue ATP or by kinetic analysis of mucins released from perfused tracheas during the first 10 minutes of ATP exposure. (escholarship.org)
  • Taking advantage of lectin recognition of mucins' carbohydrate structures, several lectins were evaluated and immobilized on streptavidin biosensors. (manchester.ac.uk)
  • Cancer-associated mucins often differ from normal mucins by presenting truncated carbohydrate chains. (elsevier.com)
  • Although some mucins are membrane-bound due to the presence of a hydrophobic membrane-spanning domain that favors retention in the plasma membrane, most mucins are secreted as principal components of mucus by mucous membranes or are secreted to become a component of saliva. (wikipedia.org)
  • Fusion of the vesicles to the plasma membrane causes release of the mucin, which as it exchanges Ca2+ for Na+ expands up to 600 fold. (wikipedia.org)