High molecular weight mucoproteins that protect the surface of EPITHELIAL CELLS by providing a barrier to particulate matter and microorganisms. Membrane-anchored mucins may have additional roles concerned with protein interactions at the cell surface.
Mucins that are found on the surface of the gastric epithelium. They play a role in protecting the epithelial layer from mechanical and chemical damage.
A gel-forming mucin that is predominantly expressed by submucosal glands of airway tissues and the SUBLINGUAL GLAND. It is one of the principal components of high molecular weight salivary mucin.
Carbohydrate antigen elevated in patients with tumors of the breast, ovary, lung, and prostate as well as other disorders. The mucin is expressed normally by most glandular epithelia but shows particularly increased expression in the breast at lactation and in malignancy. It is thus an established serum marker for breast cancer.
A transmembrane mucin that is found in a broad variety of epithelial tissue. Mucin-4 may play a role in regulating cellular adhesion and in cell surface signaling from the ERBB-2 RECEPTOR PROTEIN-TYROSINE KINASE. Mucin-4 is a heterodimer of alpha and beta chains. The alpha and beta chains result from the proteolytic cleavage of a precursor protein.
A membrane-bound mucin subtype that is primarily found in INTESTINAL MUCOSA. Two closely-related subtypes of this protein have been identified in humans.
A gel-forming mucin that is predominantly associated with the gastric epithelium.
A glandular epithelial cell or a unicellular gland. Goblet cells secrete MUCUS. They are scattered in the epithelial linings of many organs, especially the SMALL INTESTINE and the RESPIRATORY TRACT.
The viscous secretion of mucous membranes. It contains mucin, white blood cells, water, inorganic salts, and exfoliated cells.
A subcategory of mucins that contain SIALIC ACID.
The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.
Carbohydrate antigens expressed by malignant tissue. They are useful as tumor markers and are measured in the serum by means of a radioimmunoassay employing monoclonal antibodies.
Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.
The cartilaginous and membranous tube descending from the larynx and branching into the right and left main bronchi.
A histochemical technique for staining carbohydrates. It is based on PERIODIC ACID oxidation of a substance containing adjacent hydroxyl groups. The resulting aldehydes react with Schiff reagent to form a colored product.
The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.
Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.
The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.
The mucous membrane that covers the posterior surface of the eyelids and the anterior pericorneal surface of the eyeball.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
A biomineral consisting of layers of crystalized ARAGONITE, separated by interlamellar layers of organic matrix containing beta-CHITIN, and surrounded by an intercrystalline organic membrane of silk fibroin. Nacre is the iridescent substance better known as mother-of-pearl that is secreted by MOLLUSCS.
The application of LUBRICANTS to diminish FRICTION between two surfaces.
A plant genus of the family Orobanchaceae that is parasitic on the roots of other plants. Members contain the flavones, APIGENIN and LUTEOLIN.
A genus of pearl oysters in the family Pteriidae, class BIVALVIA. Both cultured and natural pearls are obtained from species in the genus. They are distinct from the distantly related, edible true oysters of the family OSTREIDAE.
The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.
A gel-forming mucin found predominantly in SMALL INTESTINE and variety of mucous membrane-containing organs. It provides a protective, lubricating barrier against particles and infectious agents.
Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.
A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.
The procedures involved in combining separately developed modules, components, or subsystems so that they work together as a complete system. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Tumors or cancer of the PANCREAS. Depending on the types of ISLET CELLS present in the tumors, various hormones can be secreted: GLUCAGON from PANCREATIC ALPHA CELLS; INSULIN from PANCREATIC BETA CELLS; and SOMATOSTATIN from the SOMATOSTATIN-SECRETING CELLS. Most are malignant except the insulin-producing tumors (INSULINOMA).
Carcinoma that arises from the PANCREATIC DUCTS. It accounts for the majority of cancers derived from the PANCREAS.
A lesion with cytological characteristics associated with invasive carcinoma but the tumor cells are confined to the epithelium of origin, without invasion of the basement membrane.
Non-optimal interval of time between onset of symptoms, identification, and initiation of treatment.
Pathological processes that tend eventually to become malignant. (From Dorland, 27th ed)
A segment of the LOWER GASTROINTESTINAL TRACT that includes the CECUM; the COLON; and the RECTUM.
The portion of the GASTROINTESTINAL TRACT between the PYLORUS of the STOMACH and the ILEOCECAL VALVE of the LARGE INTESTINE. It is divisible into three portions: the DUODENUM, the JEJUNUM, and the ILEUM.
A strain of mice widely studied as a model for cystic fibrosis. These mice are generated from embryonic stem cells in which the CFTR (cystic fibrosis transmembrane conductance regulator) gene is inactivated by gene targeting. As a result, all mice have one copy of this altered gene in all their tissues. Mice homozygous for the disrupted gene exhibit many features common to young cystic fibrosis patients, including failure to thrive, meconium ileus, and alteration of mucous and serous glands.
An autosomal recessive genetic disease of the EXOCRINE GLANDS. It is caused by mutations in the gene encoding the CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR expressed in several organs including the LUNG, the PANCREAS, the BILIARY SYSTEM, and the SWEAT GLANDS. Cystic fibrosis is characterized by epithelial secretory dysfunction associated with ductal obstruction resulting in AIRWAY OBSTRUCTION; chronic RESPIRATORY INFECTIONS; PANCREATIC INSUFFICIENCY; maldigestion; salt depletion; and HEAT PROSTRATION.
A chloride channel that regulates secretion in many exocrine tissues. Abnormalities in the CFTR gene have been shown to cause cystic fibrosis. (Hum Genet 1994;93(4):364-8)
Any pathological condition where fibrous connective tissue invades any organ, usually as a consequence of inflammation or other injury.
Infections with bacteria of the genus PSEUDOMONAS.
A natural high-viscosity mucopolysaccharide with alternating beta (1-3) glucuronide and beta (1-4) glucosaminidic bonds. It is found in the UMBILICAL CORD, in VITREOUS BODY and in SYNOVIAL FLUID. A high urinary level is found in PROGERIA.
Coloring matter from the insect Coccus cacti L. It is used in foods, pharmaceuticals, toiletries, etc., as a dye, and also has use as a microscopic stain and biological marker.
The inner membrane of a joint capsule surrounding a freely movable joint. It is loosely attached to the external fibrous capsule and secretes SYNOVIAL FLUID.
A protective layer of firm, flexible cartilage over the articulating ends of bones. It provides a smooth surface for joint movement, protecting the ends of long bones from wear at points of contact.
Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.
The clear, viscous fluid secreted by the SALIVARY GLANDS and mucous glands of the mouth. It contains MUCINS, water, organic salts, and ptylin.
The oval-shaped oral cavity located at the apex of the digestive tract and consisting of two parts: the vestibule and the oral cavity proper.
One of a set of bone-like structures in the mouth used for biting and chewing.
A solution used for irrigating the mouth in xerostomia and as a substitute for saliva.

Complete sequence of the human mucin MUC4: a putative cell membrane-associated mucin. (1/2610)

The MUC4 gene, which encodes a human epithelial mucin, is expressed in various epithelial tissues, just as well in adult as in poorly differentiated cells in the embryo and fetus. Its N-terminus and central sequences have previously been reported as comprising a 27-residue peptide signal, followed by a large domain varying in length from 3285 to 7285 amino acid residues. The present study establishes the whole coding sequence of MUC4 in which the C-terminus is 1156 amino acid residues long and shares a high degree of similarity with the rat sialomucin complex (SMC). SMC is a heterodimeric glycoprotein complex composed of mucin (ascites sialoglycoprotein 1, ASGP-1) and transmembrane (ASGP-2) subunits. The same organization is found in MUC4, where the presence of a GlyAspProHis proteolytic site may cleave the large precursor into two subunits, MUC4alpha and MUC4beta. Like ASGP-2, which binds the receptor tyrosine kinase p185(neu), MUC4beta possesses two epidermal growth factor-like domains, a transmembrane sequence and a potential phosphorylated site. MUC4, the human homologue of rat SMC, may be a heterodimeric bifunctional cell-surface glycoprotein of 2.12 micrometers. These results confer a new biological role for MUC4 as a ligand for ErbB2 in cell signalling.  (+info)

Role of retinoid receptors in the regulation of mucin gene expression by retinoic acid in human tracheobronchial epithelial cells. (2/2610)

To investigate which retinoid receptors are critical in the regulation by all-trans-retinoic acid (RA) of the mucin genes MUC2, MUC5AC and MUC5B in cultured normal human tracheobronchial epithelial (NHTBE) cells, we used pan-RAR-, pan-RXR- and RAR- isotype (alpha, beta and gamma)-selective agonists and RARalpha- and RARgamma-selective antagonists (RAR is RA receptor and RXR is retinoid X receptor). RAR-, RARalpha- and RARgamma-selective agonists strongly induced mucin mRNAs in a dose-dependent manner, while the RARbeta-selective retinoid only weakly induced mucin gene expression at very high concentrations (1 microM). The pan-RXR-selective agonist by itself did not induce mucin gene expression, but acted synergistically with suboptimal concentrations of the pan-RAR agonist. A retinoid with selective anti-activator-protein-1 activity only marginally induced mucin gene expression. The RARalpha antagonist strongly inhibited mucin gene induction and mucous cell differentiation caused by RA and by the RARalpha- and RARgamma-selective retinoids. In contrast, the RARgamma antagonist only weakly inhibited RARalpha-selective-retinoid-induced mucin gene expression, but completely blocked mucin gene expression induced by the RARgamma-selective retinoid. Our studies indicate that RARalpha is the major retinoid receptor subtype mediating RA-dependent mucin gene expression and mucous cell differentiation, but that the RARgamma isotype can also induce mucin genes. Furthermore these studies suggest that RARbeta is probably not (directly) involved in RA-induced mucin gene expression.  (+info)

Physical characterization of a low-charge glycoform of the MUC5B mucin comprising the gel-phase of an asthmatic respiratory mucous plug. (3/2610)

We have previously noted that sequential extraction of an asthmatic mucous exudate with 6 M guanidinium chloride yielded a fraction of the mucins that were most resistant to solubilization and of high Mr [Sheehan, Richardson, Fung, Howard and Thornton (1995) Am. J. Respir. Cell Mol. Biol. 13, 748-756]. Here we show that this mucin fraction is dominated (at least 96% of the total) by the low-charge glycoform of the MUC5B gene product. Seen in the electron microscope the mucins appeared mainly as compact 'island' structures composed of linear threads often emanating from globular 'nodes' rather than the discrete linear threads more typical of mucins that we have previously described. The effect of reducing agents was as expected for other gel-forming mucins, i.e. reduced subunits or monomers of Mr 3x10(6)) were produced within 15 min of treatment. Kinetic experiments on the cleavage of the intact mucins with the proteinase trypsin indicated two clear regimes of fragmentation. An initial rapid cleavage generated mucins ranging from Mr=4x10(6) to 30x10(6) that in the electron microscope appeared as polydisperse threads (500-3000 nm in length), similar to normal and other respiratory mucins that we have previously characterized. A subsequent slower fragmentation over many hours yielded a major fragment of Mr 3x10(6) and length 200-600 nm, very similar in size and Mr to the subunits obtained by reduction. The results suggest that the MUC5B mucin is assembled, first into polydisperse linear threads, which are then linked together via a protein-mediated process. This might involve part of the mucin polypeptide or an as yet unidentified protein(s). The high proteinase susceptibility of the linkage suggests that it might be a point of control for mucin size and thus mucus rheology.  (+info)

Salivary mucin MG1 is comprised almost entirely of different glycosylated forms of the MUC5B gene product. (4/2610)

The MG1 population of mucins was isolated from human whole salivas by gel chromatography followed by isopycnic density gradient centrifugation. The reduced and alkylated MG1 mucins, separated by anion exchange chromatography, were of similar size (radius of gyration 55-64 nm) and molecular weight (2.5-2.9 x 10(6) Da). Two differently-charged populations of MG1 subunits were observed which showed different reactivity with monoclonal antibodies to glycan epitopes. Monosaccharide and amino acid compositional analyses indicated that the MG1 subunits had similar glycan structures on the same polypeptide. An antiserum recognizing the MUC5B mucin was reactive across the entire distribution, whereas antisera raised against the MUC2 and MUC5AC mucins showed no reactivity. Western blots of agarose gel electrophoresis of fractions across the anion exchange distribution indicated that the polypeptide underlying the mucins was the product of the MUC5B gene. Amino acid analysis and peptide mapping performed on the fragments produced by trypsin digestion of the two MG1 populations yielded data similar to that obtained for MUC5B mucin subunits prepared from respiratory mucus (Thornton et al., 1997) and confirmed that the MUC5B gene product was the predominant mucin polypeptide present. Isolation of the MG1 mucins from the secretions of the individual salivary glands (palatal, sublingual, and submandibular) indicate that the palatal gland is the source of the highly charged population of the MUC5B mucin.  (+info)

The sialylation of bronchial mucins secreted by patients suffering from cystic fibrosis or from chronic bronchitis is related to the severity of airway infection. (5/2610)

Bronchial mucins were purified from the sputum of 14 patients suffering from cystic fibrosis and 24 patients suffering from chronic bronchitis, using two CsBr density-gradient centrifugations. The presence of DNA in each secretion was used as an index to estimate the severity of infection and allowed to subdivide the mucins into four groups corresponding to infected or noninfected patients with cystic fibrosis, and to infected or noninfected patients with chronic bronchitis. All infected patients suffering from cystic fibrosis were colonized by Pseudomonas aeruginosa. As already observed, the mucins from the patients with cystic fibrosis had a higher sulfate content than the mucins from the patients with chronic bronchitis. However, there was a striking increase in the sialic acid content of the mucins secreted by severely infected patients as compared to noninfected patients. Thirty-six bronchial mucins out of 38 contained the sialyl-Lewis x epitope which was even expressed by subjects phenotyped as Lewis negative, indicating that at least one alpha1,3 fucosyltransferase different from the Lewis enzyme was involved in the biosynthesis of this epitope. Finally, the sialyl-Lewis x determinant was also overexpressed in the mucins from severely infected patients. Altogether these differences in the glycosylation process of mucins from infected and noninfected patients suggest that bacterial infection influences the expression of sialyltransferases and alpha1,3 fucosyltransferases in the human bronchial mucosa.  (+info)

Sulphation and secretion of the predominant secretory human colonic mucin MUC2 in ulcerative colitis. (6/2610)

BACKGROUND: Decreased synthesis of the predominant secretory human colonic mucin (MUC2) occurs during active ulcerative colitis. AIMS: To study possible alterations in mucin sulphation and mucin secretion, which could be the cause of decreased mucosal protection in ulcerative colitis. METHODS: Colonic biopsy specimens from patients with active ulcerative colitis, ulcerative colitis in remission, and controls were metabolically labelled with [35S]-amino acids or [35S]-sulphate, chase incubated and analysed by SDS-PAGE, followed by quantitation of mature [35S]-labelled MUC2. For quantitation of total MUC2, which includes non-radiolabelled and radiolabelled MUC2, dot blotting was performed, using a MUC2 monoclonal antibody. RESULTS: Between patient groups, no significant differences were found in [35S]-sulphate content of secreted MUC2 or in the secreted percentage of either [35S]-amino acid labelled MUC2 or total MUC2. During active ulcerative colitis, secretion of [35S]-sulphate labelled MUC2 was significantly increased twofold, whereas [35S]-sulphate incorporation into MUC2 was significantly reduced to half. CONCLUSIONS: During active ulcerative colitis, less MUC2 is secreted, because MUC2 synthesis is decreased while the secreted percentage of MUC2 is unaltered. Furthermore, sulphate content of secreted MUC2 is unaltered by a specific compensatory mechanism, because sulphated MUC2 is preferentially secreted while sulphate incorporation into MUC2 is reduced.  (+info)

Mucin expression and function in the female reproductive tract. (7/2610)

Reproductive tract epithelia are characterized by the presence of a thick, apical glycocalyx. This glycoprotein coat is drastically reduced in the uterus of many species during the time of embryo implantation. Recent studies indicate that mucin glycoproteins constitute a large proportion of the apical glycocalyx. One of these mucins, Muc-1, has particularly important functions at the luminal surface of the uterus and other female reproductive tract tissues. Muc-1 appears to play a dominant role in maintaining a functionally non-receptive uterine surface with regard to blastocyst attachment. Conversion to a receptive uterine state is brought about by the concerted actions of ovarian steroid hormones that in several species also strongly modulate Muc-1 protein and mRNA expression. Muc-1 also appears to serve a general function in protecting reproductive tract mucosa since Muc-1 null mice are particularly prone to bacterial infection. Collectively, these studies indicate that mucins, including Muc-1, play important barrier roles in reproductive processes and protection from bacterial pathogenesis in the female reproductive tract.  (+info)

pH-dependent conformational change of gastric mucin leads to sol-gel transition. (8/2610)

We present dynamic light scattering (DLS) and hydrophobic dye-binding data in an effort to elucidate a molecular mechanism for the ability of gastric mucin to form a gel at low pH, which is crucial to the barrier function of gastric mucus. DLS measurements of dilute mucin solutions were not indicative of intermolecular association, yet there was a steady fall in the measured diffusion coefficient with decreasing pH, suggesting an apparent increase in size. Taken together with the observed rise in depolarized scattering ratio with decreasing pH, these results suggest that gastric mucin undergoes a conformational change from a random coil at pH >/= 4 to an anisotropic, extended conformation at pH < 4. The increased binding of mucin to hydrophobic fluorescent with decreasing pH indicates that the change to an extended conformation is accompanied by exposure of hydrophobic binding sites. In concentrated mucin solutions, the structure factor S(q, t) derived from DLS measurements changed from a stretched exponential decay at pH 7 to a power-law decay at pH 2, which is characteristic of a sol-gel transition. We propose that the conformational change facilitates cross-links among mucin macromolecules through hydrophobic interactions at low pH, which in turn leads to a sol-gel transition when the mucin solution is sufficiently concentrated.  (+info)

Mucins are large glycoproteins that cover epithelial surfaces of the body. All mucins contain at least one PTS domain, a region rich in proline, threonine and serine. Mucins are also characterized by von Willebrand D (VWD) domains or SEA domains. We have developed computational methods to identify mucin genes and proteins based on these properties of the proteins. Using such methods we are able to characterize different organisms where genome sequence is available with respect to their mucin repertoire. We have here made a comprehensive analysis of potential mucins encoded by the chicken (Gallus gallus) genome. Three transmembrane mucins (Muc4, Muc13, and Muc16) and four gel-forming mucins (Muc6, Muc2, Muc5ac, and Muc5b) were identified. The gel-forming mucins are encoded within a locus similar to the corresponding human mucins. However, the chicken has an additional gene inserted between Muc2 and Muc5ac that encodes the the α-subunit of ovomucin, a protein similar to Muc2, but it is lacking a PTS
There is intense interest in how bacteria interact with mucin glycoproteins in order to colonise mucosal surfaces. In this study, we have assessed the feasibility of using recombinant mucin glycoproteins to study the interaction of the gastric pathogen Helicobacter pylori with MUC5AC, a mucin which the organism exhibits a distinct tropism for. Stable clonal populations of cells expressing a construct encoding for a truncated version of MUC5AC containing N- and C-termini interspersed with two native tandem repeat sequences (N + 2TR + C) were generated. Binding of H. pylori to protein immunoprecipitated from cell lysates and supernatants was assessed. High molecular weight mucin could be detected in both cell lysates and supernatants of transfected cells. Recombinant protein formed high molecular weight oligomers, was both N and O glycosylated, underwent cleavage similar to native MUC5AC and was secreted from the cell. H. pylori bound better to secreted mucin than intracellular mucin suggesting that
Ulcerative colitis (UC), a common form of inflammatory bowel disease, is a multifactorial disorder with significant genetic influence. Recently, evidence of linkage on chromosome 7q near the intestinal mucin gene MUC3 was reported by an affected sib-pair analysis. Previous reports indicate a possible mucin abnormality in UC patients, but whether genetic differences in a specific mucin gene are associated with UC is unknown. Here we analysed polymorphisms of variable number of tandem repeats (VNTRs) within this gene using DNAs obtained from 243 Japanese (75 patients with UC and 168 controls), and to confirm the result we undertook a two-stage examination using 328 Caucasian samples (72 and 85 with UC in the first and second stages, respectively, and 171 controls). When the frequency of patients carrying one or two rare VNTR alleles was compared with that of controls, a significant increase was found first in Japanese patients (odds ratio 2.72, 95% CI 1.17-6.32, P = 0. 0308). In Caucasians, the odds ratio
McGuckin M.A., Wright G. and Ward B.G. (1991) Expression of a polymorphic epithelial mucin antigen defined by the monoclonal antibody BC2 in ovarian carcinoma: Use of the BC2 antibody for the detection of micrometastases. American Journal of Clinical Pathology, 96 1: 46-52. ...
0005] The glycocalyx of the corneal epithelium contains the transmembrane mucin (glycosylated glycoproteins present in the glycocalyx), MUC1, MUC4, and MUC16. Mucins are high molecular weight Glycoproteins that provide a protective layer on corneal epithelial surfaces and are involved in cell-cell interactions, signaling, and metastasis. MUCIN 1, a cell surface associated (MUC 1) or polymorphic epithelial mucin (PEM) is a mucin, the protein part of which is encoded by the MUC1 gene in humans. Mucin proteins penetrate the membranes of epithelial cells, on the inner surface of the intestine and other organs. Mucin protects the body from infection by binding to pathogens. These membrane mucins interact with soluble, secreted, gel-forming mucin produced by the goblet cells (MUC5AC) and also with others like MUC2. The lacrimal gland also secretes MUC7 into the tear film. These soluble mucin in the tear film moves freely, (made possible by blinking and electrostatic repulsion from the negatively ...
TY - JOUR. T1 - Intestinal trefoil factor (TFF-3) and extracellular signal-regulated kinase (ERK) in cholangiocarcinoma. AU - Ailawadhi, Sikander. AU - Nagase, Hiroki. AU - Khoury, Thaer. AU - Yu, Jihnhee. AU - Tan, Dongfeng. AU - Black, Jennifer. AU - Brattain, Michael. AU - Javle, Milind. PY - 2007/7. Y1 - 2007/7. N2 - Background/Aims: The mucin-associated trefoil factor (TFF) peptides are integral to cytoprotection. TFF-3 is aberrantly expressed in colorectal and hepatocellular cancer and associated with an invasive phenotype. TFF-3 is also expressed in normal biliary epithelium. However, its role in biliary cancers is unknown. The biological effects of TFFs may result from EGFR, PI3 kinase, COX-2 and STAT-mediated signaling. We investigated the expression of TFF-3, Erk, Akt, EGFR and COX-2 in biliary cancer. Methodology: Twenty-four consecutive cases of cholangiocarcinoma treated from 1996-2002 were studied. Immunohistochemistry was performed using monoclonal antibodies to TFF-3, EGFR, ...
The sustained delivery of both hydrophobic and hydrophilic drugs from hydrogels remains a challenge that requires the design of complex multifunctional synthetic polymers. In this work we exploit mucin glycoproteins, the gel-forming building blocks of natural mucus to deliver both hydrophobic and hydrophilic drugs. Mucins are a family of high molecular weight proteins which are densely glycosylated. Their central protein backbone contains hydrophobic and charged domains, while the mucin-associated glycans provide hydrogen bonding capabilities, high hydration, and negative charges. The biochemical versatility of mucins represents potential binding sites for certain drugs. If assembled into hydrogels, mucins could prevent drugs from freely diffusing out, leading to their sustained delivery. We generated methacrylated mucins which assembled into a covalently crosslinked mucin hydrogel when exposed to UV light. The rheological properties of the mucin gels were dominated by an elastic component, and ...
In the present study, we examined whether eosinophils induce MUC5AC mucin synthesis in airway epithelial cells. Our results showed that activated human eosinophils caused MUC5AC mucin synthesis in NCI-H292 cells. Similarly, the supernatant of activated eosinophils induced MUC5AC gene and protein synthesis in NCI-H292 cells, indicating that this effect of activated eosinophils was related to a secreted product.. Because EGFR activation is reported to cause mucin synthesis (13), we examined whether EGFR activation is required for activated eosinophil-induced mucin synthesis. Activated eosinophil supernatant induced EGFR phosphorylation in NCI-H292 cells, and selective inhibitors of EGFR tyrosine kinase blocked activated eosinophil-induced EGFR phosphorylation and MUC5AC synthesis completely; a selective PDGFR inhibitor (AG1295) and a negative control for tyrphostins (AG9) were without effect. These findings implicate EGFR tyrosine kinase phosphorylation in mucin synthesis induced by activated ...
Goblet cells reside throughout the gastrointestinal (GI) tract and are responsible for the production and preservation of a protective mucus blanket by synthesizing and secreting high molecular weight glycoproteins known as mucins. The concept of the mucus layer functioning as a dynamic protective barrier is suggested by studies showing changes in mucins in inflammatory conditions of the GI tract, by the altered goblet cell response in germ-free animals, and by the enhanced mucus secretion seen in response to infections. The mucin-containing mucus layer coating the GI epithelium is the front line of innate host defense. Mucins are likely to be the first molecules that invading pathogens interact with at the cell surface and thus, can limit binding to other glycoproteins and neutralize the pathogen. This review will focus on what is known about goblet cell response in various GI infections and the regulatory networks that mediate goblet cell function and mucin production in response to intestinal insults
TY - JOUR. T1 - Single molecule study of heterotypic interactions between mucins possessing the Tn cancer antigen. AU - Haugstad, Kristin E.. AU - Stokke, Bjørn T.. AU - Brewer, Curtis F.. AU - Gerken, Thomas A.. AU - Sletmoen, Marit. PY - 2015. Y1 - 2015. N2 - Mucins are linear, heavily O-glycosylated proteins with physiological roles that include cell signaling, cell adhesion, inflammation, immune response and tumorgenesis. Cancer-associated mucins often differ from normal mucins by presenting truncated carbohydrate chains. Characterization of the binding properties of mucins with truncated carbohydrate side chains could thus prove relevant for understanding their role in cancer mechanisms such as metastasis and recognition by the immune system. In this work, heterotypic interactions of model mucins that possess the Tn (GalNA-cαThr/Ser) and T (Galβ1-3GalNAcαThr/Ser) cancer antigens derived from porcine submaxillary mucin (PSM) were studied using atomic force microscopy. PSM possessing only ...
TY - JOUR. T1 - Interaction of bile salt and phospholipids with bovine submaxillary mucin. AU - Wiedmann, T. S.. AU - Deye, C.. AU - Kallick, D.. PY - 2001. Y1 - 2001. N2 - Purpose. The purpose of this study was to determine the distribution and diffusion of sodium taurocholate-phospholipid micelles with mucin in order to provide the foundation for understanding the transport of ingested fat and poorly water-soluble drugs through the intestinal mucous layer. Methods. Sodium taurocholate NaTC was dispersed with egg phosphatidylcholines PC to yield mixed micelles of a specific size and concentration. A preliminary study was conducted to determine the time required for equilibration of PC/TC micellar solutions with mucin. PC/TC micellar solutions were dialyzed against fixed and variable concentrations of bovine submaxillary mucin after which the concentration of PC and NaTC was measured by an assay for total phosphorus and by HPLC, respectively. In addition, a quantitative assay of TC and PC by ...
Figure 1. Yearly number of publication related to mucins as reported by scicurve.com, using the keyword mucins.. This is because mucin research spans over many fields. Biologists have been intrigued by mucins since long. But more recently, microbiologists studying our microbiome have new reasons to find this class of biopolymers particularly exciting. For instance, they realized that intestinal mucus plays key roles in the behavior of both pathogenic and commensal bacteria. This will surely lead to many more microbiology studies with mucus/mucins as a key component (1,2). Similarly, recent findings in immunology describing interactions between dendritic cells and mucin-associated glycans will also attract attention to secreted mucins in that field (3).. Some physicists and biophysicists are also fascinated by mucins. Tribologists are trying to understand and recreate mucins efficient lubricity that is so precious for a healthy mouth and gastrointestinal tract (4,5). The field of oral drug ...
The mucin-type glycoprotein podoplanin is specifically expressed by lymphatic but not blood vascular endothelial cells in culture and in tumor-associated lymphangiogenesis, and podoplanin deficiency results in congenital lymphedema and impaired lymphatic vascular patterning. indicated by granulosa cells in regular ovarian follicles highly, and by ovarian granulosa and dysgerminomas cell tumors. Although podoplanin was absent from regular human being epidermis mainly, its manifestation was induced in 22 of 28 squamous cell carcinomas studied strongly. These findings recommend a potential part of podoplanin in tumor development, plus they also determine the 1st commercially obtainable antibody for the precise staining of a precise lymphatic marker in archival human being tissue sections, allowing more widespread research of tumor lymphangiogenesis in human cancers thereby. Lymphatic vessels play a significant part in the maintenance of cells homeostasis1 and in the transportation of immune system ...
TY - JOUR. T1 - Cystic fibrosis and the relationship between mucin and chloride secretion by cultures of human airway gland mucous cells. AU - Finkbeiner, Walter E.. AU - Zlock, Lorna T.. AU - Morikawa, Masatoshi. AU - Lao, Anna Y.. AU - Dasari, Vijay. AU - Widdicombe, Jonathan. PY - 2011/10. Y1 - 2011/10. N2 - We investigated how cystic fibrosis (CF) alters the relationship between Cl - and mucin secretion in cultures of non-CF and CF human tracheobronchial gland mucous (HTGM and CFTGM, respectively) cells. Biochemical studies showed that HTMG cells secreted typical airway mucins, and immunohistochemical studies showed that these cells expressed MUC1, MUC4, MUC5B, MUC8, MUC13, MUC16, and MUC20. Effects of cumulative doses of methacholine (MCh), phenylephrine (Phe), isoproterenol (Iso), and ATP on mucin and Cl - secretion were studied on HTGM and CFTGM cultures. Baseline mucin secretion was not significantly altered in CFTGM cells, and the increases in mucin secretion induced by mediators were ...
Mucins (/ˈmjuːsɪn/) are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by epithelial tissues in most animals.[1] Mucins key characteristic is their ability to form gels; therefore they are a key component in most gel-like secretions, serving functions from lubrication to cell signalling to forming chemical barriers.[1] They often take an inhibitory role.[1] Some mucins are associated with controlling mineralization, including nacre formation in mollusks,[2] calcification in echinoderms[3] and bone formation in vertebrates.[4] They bind to pathogens as part of the immune system. Overexpression of the mucin proteins, especially MUC1, is associated with many types of cancer.[5] Although some mucins are membrane-bound due to the presence of a hydrophobic membrane-spanning domain that favors retention in the plasma membrane, most mucins are secreted as principal components of mucus by mucous membranes or are secreted to become a component of saliva. ...
TY - JOUR. T1 - Pattern of mucin gene expression in normal and neoplastic lung tissues. AU - Seregni, E.. AU - Botti, C.. AU - Lombardo, C.. AU - Cantoni, A.. AU - Bogni, A.. AU - Cataldo, I.. AU - Bombardieri, E.. PY - 1996. Y1 - 1996. N2 - This work evaluates the expression in lung cancer of the most well characterized mucin genes (MUC1, MUC2, MUC3) and of the recently described MUC4 in lung tissues, to check a correlation between the expression of any particular gene and this tumor. Hybridization with synthetic oligonucleotides obtained from a part of the sequences of MUC1, MUC2, MUC3 and MUC4, was performed on blotted RNA from 18 lung cancer tissue specimens and from 10 normal tissues samples taken, when possible, from the normal lung counterpart. By means of Northern blot analysis MUC1 revealed to be the most expressed mucin gene in lung cancer, followed by MUC4; by contrast, the expression of MUC2 and MUC3 was almost undetectable in all cancer specimens. The intensity of expression of MUC1 ...
Liu, C., Comparison of structural characteristics of major membrane-bound mucins from porcine milk and intestinal brush borders. (2003). Dairy Science Publication Database. 336 ...
Salivary secretions contain two distinct mucins, a high molecular weight mucin named MG1 and a low molecular weight mucin named MG2. Mucins protect oral surface...
TY - JOUR. T1 - Effects of a novel nonantibiotic macrolide, EM900, on cytokine and mucin gene expression in a human airway epithelial cell line. AU - Otsu, Kazuya. AU - Ishinaga, Hajime. AU - Suzuki, Shinya. AU - Sugawara, Akihiro. AU - Sunazuka, Toshiaki. AU - Omura, Satoshi. AU - Jono, Hirofumi. AU - Takeuchi, Kazuhiko. PY - 2011/12. Y1 - 2011/12. N2 - Background/Aims: Long-term macrolide therapy is an effective treatment for chronic sinusitis and diffuse panbronchiolitis. However, long-term use of macrolides may promote the growth of drug-resistant bacteria; therefore, development of macrolides with no antibacterial action is desirable. A new erythromycin (EM) derivative, (8R,9S)- 8,9-dihydro-6,9-epoxy-8,9- anhydropseudoerythromycin A (EM900), does not possess antibacterial action. Methods: To determine whether EM900 induced a clinically relevant anti-inflammatory response and repressed mucin gene expression in cells derived from human airway epithelia, we assessed the effects of EM900 on ...
Mucins are high-molecular weight glycoproteins (0.25-20 MDa) containing one or more domains that are heavily O-glycosylated. Their implications as targets for cancer treatment have increased the interest in these glycoproteins, mainly in the fields of vaccines and antibodies. However, mucins present high heterogeneity, posing challenges that affect purification processes and quality control analysis. In that sense, it is necessary to develop and improve downstream processes and analytical methods to characterize these products. Here a tool based on biolayer interferometry analysis to improve mucins detection and quantification in a fast, simple and label free-way is presented. Taking advantage of lectin recognition of mucins carbohydrate structures, several lectins were evaluated and immobilized on streptavidin biosensors. Different assay conditions were optimized and the most suitable lectin, Aleuria aurantia lectin (AAL), was selected. Bovine Submaxillary Gland and human MUC5B mucins were ...
TY - JOUR. T1 - Enhanced self-association of mucins possessing the T and Tn carbohydrate cancer antigens at the single-molecule level. AU - Haugstad, Kristin E.. AU - Gerken, Thomas A.. AU - Stokke, Bjørn T.. AU - Dam, Tarun K.. AU - Brewer, C. Fred. AU - Sletmoen, Marit. PY - 2012/5/14. Y1 - 2012/5/14. N2 - Mucins are linear O-glycosylated glycoproteins involved in inflammation, cell adhesion, and tumorigenesis. Cancer-associated mucins often possess increased expression of the T (Galβ1,3GalNAcαThr/Ser) and Tn (GalNAcαThr/Ser) cancer antigens, which are diagnostic markers for several cancers, including colon cancer. We have used AFM based single-molecule forced unbinding under near physiological conditions to investigate the self-interactions between porcine submaxillary mucin (PSM) as well as between PSM analogs possessing various carbohydrates including the T- and Tn-antigen. Distributions of unbinding forces and corresponding force loading rates were determined for force loading rates ...
We have previously reported the establishment of cytotoxic T-cell lines from pancreatic cancer patients, by continuously stimulating tumor-draining lymph node cells with allogeneic pancreatic tumor cell lines. After the preliminary characterization of their phenotype and tumor specificity, detailed studies performed with one of the cell lines, W.D., show that it recognizes a specific antigen, a large and heavily glycosylated mucin molecule, expressed on pancreatic and breast tumors and tumor cell lines. Although this recognition appears major histocompatibility complex (MHC)-unrestricted, the antigen receptor used by the cytotoxic T cell is the alpha/beta heterodimer, typically found on MHC-restricted T cells. The target antigen is atypical, however, in its ability to directly bind and activate the T cells in the absence of self MHC, presumably by abundant and regularly repeated antigenic epitopes. These findings are important because they demonstrate a specific T-cell response against a human ...
LAA413Hu71, Biotin-Linked Polyclonal Antibody to Mucin 1 (MUC1), Homo sapiens (Human), Labelled antibody, CD227, EMA, H23AG, KL-6, PEM, PEMT, PUM, CA15-3, Cancer antigen 15-3, Carcinoma-associated mucin, Episialin, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Designed by Cloud-Clone Corp.
Mucins are high molar mass glycoproteins that assume an extended conformation and can assemble into mucus hydrogels that protect our mucosal epithelium. In nature, the challenging task of generating a mucus layer, several hundreds of micrometers in thickness, from micrometer-sized cells is elegantly solved by the condensation of mucins inside vesicles and their on-demand release from the cells where they suddenly expand to form the extracellular mucus hydrogel. We aimed to recreate and control the process of compaction for mucins, the first step toward a better understanding of the process and creating biomimetic in vivo delivery strategies of macromolecules. We found that by adding glycerol to the aqueous solvent, we could induce drastic condensation of purified mucin molecules, reducing their size by an order of magnitude down to tens of nanometers in diameter. The condensation effect of glycerol was fully reversible and could be further enhanced and partially stabilized by cationic ...
In gene-targeted mouse models for cystic fibrosis (CF), the disease is mainly manifested by mucus obstruction in the intestine. To explore the mucus composition, mucins insoluble and soluble in 6 M guanidinium chloride were purified by three rounds of isopycnic ultracentrifugation from the small and large intestines of CF mice (Cftr(m1UNC)/Cftr(m1UNC)) and compared with wild-type mice. The amino acid composition was typical of that for mucins and showed increased amounts of the insoluble (2.5-fold increase) and soluble (7-fold increase) mucins in the small intestine of the CF mice compared with wild-type mice. Mucins from the large intestine of both wild-type and CF mice showed a high but constant level of fucosylation. In contrast, the insoluble and soluble mucins of the small intestine in CF mice revealed a large increase in fucose, whereas those of wild-type mice contained only small amounts of fucose. This increased fucosylation was analysed by releasing the O-linked oligosaccharides ...
View more ,Efficient separation of mucins (200 kDa-2 MDa) was demonstrated using gradient SDS agarose/polyacrylamide composite gel electrophoresis (SDS-AgPAGE). Inclusion of urea (SDS-UAgPAGE) in the gels casting were shown to have no effect on the migration of mucins in the gel and allowed casting of gel at room temperature. This simplified the procedure for multiple casting of agarose polyacrylamide gradients and increased reproducibility of these gels. Hence, the implementation of urea makes the technique applicable for high throughput isolation and screening of mucin oligosaccharides by LC-MS after releasing the oligosaccharides from isolated, blotted mucin subpopulations. It was also shown that the urea addition had no effect on other supporting applications such as western and lectin blotting. In addition, identification of the mucin protein after tryptic digestion and LC-MS was possible and no protein carbamylation due to the presence of urea in the gel was detected. LC-MS software ...
An unusual case of a mucin secreting benign microfollicular adenoma of the thyroid in a 30 year old euthyroid woman is reported. Histologically, the lesion was characterised by follicular cells with the appearance of signet ring cells. Histochemistry showed the mucin content of these cells to consist uniformly of sulphated acid mucins; positive thyroglobulin immunostaining was also shown. The published work on primary mucin secreting tumours of the thyroid gland is reviewed. Dual differentiation is thought to be responsible for combined mucin secretion and hormone production in this type of neoplasm.. ...
Efficient separation of mucins (200 kDa-2 MDa) was demonstrated using gradient SDS agarose/polyacrylamide composite gel electrophoresis (SDS-AgPAGE). Inclusion of urea (SDS-UAgPAGE) in the gels casting were shown to have no effect on the migration of mucins in the gel and allowed casting of gel at room temperature. This simplified the procedure for multiple casting of agarose polyacrylamide gradients and increased reproducibility of these gels. Hence, the implementation of urea makes the technique applicable for high throughput isolation and screening of mucin oligosaccharides by LC-MS after releasing the oligosaccharides from isolated, blotted mucin subpopulations. It was also shown that the urea addition had no effect on other supporting applications such as western and lectin blotting. In addition, identification of the mucin protein after tryptic digestion and LC-MS was possible and no protein carbamylation due to the presence of urea in the gel was detected. LC-MS software developed for ...
Author(s): Ren, Binhui; Azzegagh, Zoulikha; Jaramillo, Ana M; Zhu, Yunxiang; Pardo-Saganta, Ana; Bagirzadeh, Rustam; Flores, Jose R; Han, Wei; Tang, Yong-Jun; Tu, Jing; Alanis, Denise M; Evans, Christopher M; Guindani, Michele; Roche, Paul A; Rajagopal, Jayaraj; Chen, Jichao; Davis, C William; Tuvim, Michael J; Dickey, Burton F | Abstract: Airway mucin secretion is important pathophysiologically and as a model of polarized epithelial regulated exocytosis. We find the trafficking protein, SNAP23, selectively expressed in secretory cells compared to ciliated and basal cells of airway epithelium by immunohistochemistry and fluorescence activated cell sorting, suggesting that SNAP23 functions in regulated but not constitutive epithelial secretion. Heterozygous SNAP23 deletant mutant mice show spontaneous accumulation of intracellular mucin, indicating a defect in baseline secretion. However mucins are release from perfused tracheas of mutant and wild-type mice at the same rate, suggesting that increased
The selectin family of cell adhesion molecules mediates the tethering and rolling of leukocytes on blood vessel endothelium. It has been postulated that the molecular basis of this highly dynamic adhesion is the low affinity and rapid kinetics of selectin interactions. However, affinity and kinetic analyses of monomeric selectins binding their natural ligands have not previously been reported. Leukocyte selectin (L-selectin, CD62L) binds preferentially to O-linked carbohydrates present on a small number of mucin-like glycoproteins, such as glycosylation-dependent cell adhesion molecule-1 (GlyCAM-1), expressed in high endothelial venules. GlyCAM-1 is a soluble secreted protein which, following binding to CD62L, stimulates beta2-integrin-mediated adhesion of lymphocytes. Using surface plasmon resonance, we show that a soluble monomeric form of CD62L binds to purified immobilized GlyCAM-1 with a dissociation constant (Kd) of 108 microM. CD62L dissociates from GlyCAM-1 with a very fast dissociation rate
Latest reports claim that autoantibodies directed to glycosylated mucins aberrantly, specifically MUC4 and MUC1, are located in individuals with colorectal cancer. Rosetta2 (Novagen), nickel purified using NiNTA agarose (Qiagen, Venlo, NL) accompanied by HPLC purification before and after O-glycosylation. Items were analyzed by MALDI-TOF and SDS-PAGE mass spectrometry on the Voyager-DE? PRO workstation (Applied Biosystems, Lifestyle Technology, Carlsbad, CA) using 2,5-dihydroxybenzoic acidity (Sigma, St. Louis, Missouri) as matrix. AB1010 Microarray MUC1, MUC4 and MUC2 glycopeptides and glycoproteins were printed on Schott Nexterion? Glide H MPX 48 (Schott AG, Mainz, Germany) as previously defined.18 In brief, triplicates of most peptides, in 150 mM sodium phosphate pH 8.5 with 0.005% CHAPS, were printed on the BioRobotics MicroGrid II spotter (Genomics Solution) having a 0.21-mm pitch using Stealth 3B Micro Spotting Pins (Telechem International ArrayIt Division). After printing, slides had been ...
TY - JOUR. T1 - Mechanistic and functional shades of mucins and associated glycans in colon cancer. AU - Pothuraju, Ramesh. AU - Krishn, Shiv Ram. AU - Gautam, Shailendra K.. AU - Pai, Priya. AU - Ganguly, Koelina. AU - Chaudhary, Sanjib. AU - Rachagani, Satyanarayana. AU - Kaur, Sukhwinder. AU - Batra, Surinder K.. PY - 2020/3. Y1 - 2020/3. N2 - Mucus serves as the chief protective barrier against pathogenic and mechanical insults in respiratory, gastrointestinal, and urogenital tracts. Altered mucin expression, the major component of mucus, in conjunction with differential glycosylation has been strongly associated with both benign and malignant pathologies of colon. Mucins and their associated glycans arbitrate their impact sterically as well as mechanically by altering molecular and microbial spectrum during pathogenesis. Mucin expression in normal and pathological conditions is regulated by nonspecific (dietary factors and gut microbiota) and specific (epigenetic and transcriptional) ...
To investigate the genetic identities of the mucins secreted in cystic fibrosis (CF) airways, sputum was collected from five individuals. Samples were separated into gel and sol phases by high-speed centrifugation and the gel phase was extracted in 6 M guanidinium chloride. The insoluble residue remaining after extraction of the gel phase was brought into solution by reduction/alkylation. Density-gradient centrifugation in CsCl revealed polydisperse distributions of sialic acid-containing mucins in the gel phase, insoluble residue and sol phase fractions and the degree of variation between the different individuals was low. Antibodies recognizing MUC5AC and MUC5B identified these mucins in each of the fractions. MUC2, however, was present only as a component of the insoluble residue from the gel which accounted for less than 4% by mass of the total mucins. MUC5B and MUC5AC from the gel phase were large oligomeric species composed of disulphide-bond linked subunits and MUC5B was present as two ...
Mucins are the primary macromolecular component of mucus-natures natural lubricant-although they are poorly characterised heterogeneous substances. Recent advances in hydrodynamic methodology now offer the opportunity for gaining a better understanding of their solution properties. In this study a combination of such methods was used to provide increased understanding of a preparation of porcine intestinal mucin (PIM), MUC2 mucin, in terms of both heterogeneity and quantification of conformational flexibility. The new sedimentation equilibrium algorithm SEDFIT-MSTAR is applied to yield a weight average (over the whole distribution) molar mass of 7.1 × 106 g mol−1, in complete agreement with size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS), which yielded a value of 7.2 × 106 g mol−1. Sedimentation velocity profiles show mucin to be very polydisperse, with a broad molar mass distribution obtained using the Extended Fujita algorithm, consistent with the ...
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The aim of this study was to assess the profile of mucins (MUC1, MUC2, MUC5AC) in the intestinal metaplasia (IM) of the gastric mucosa through the immunohistochemical method.. Methods: To identify the metaplastic areas in the gastric mucosa, chromoendoscopy was employed using 0.5% solution of methylene blue. The expression of the profile of the mucins was determined using immunohistochemistry with MUC1, MUC5AC, and MUC2 antibodies (clone Ma695, clone CLH2, Ccp58 and CLH5, Novocastra Great Britain).. Results: In the regions adjacent to the adenocarcinoma and neoplastic modified cells, a visible weak expression of MUC2 and MUC5AC was observed. In the case of complete IM, a visibly maximum MUC2 expression was observed in the goblet cells; thus, the MUC5AC, MUC1, and MUC6 marking were absent in the columnar epitheliocytes with the brush border. In the case of incomplete IM, along with the positive MUC2 markings of the goblet cells, the presence of gastric mucin (MUC5AC) has been observed in 25% of ...
Looking for mucin clot test? Find out information about mucin clot test. see glycoprotein glycoprotein , organic compound composed of both a protein and a carbohydrate joined together in covalent chemical linkage. These... Explanation of mucin clot test
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From repairing skin damage and providing anti-aging benefits to soothing irritated skin and fading acne scars, snail mucin, or snail secretion filtrate, offers so many benefits. Its truly a versatile ingredient, suitable for all skin types and concerns, and plays well with other ingredients. Studies have shown that snail mucin even helps with atopic dermatitis. In fact, even before snail mucin became popular as a skin care ingredient, the main component of snail mucin, Chondroiton sulfate, was recognized for its ability to help improve arthritis, liver diseases, nephritis, and fatigue.. But have you ever wondered where snail mucin comes from? You might be thinking that snails are plucked from their natural habitats and forced to produce mucin, which eventually ends up in pretty packaging on store shelves. Or you might have assumed that snail mucin is artificially created in a petri dish and then mass produced to meet the high demand for snail mucin. The answer is none of the above. Read on for ...
MUC5AC (mucin 5AC, oligomeric mucus/gel-forming), Authors: Raquel Mejías-Luque, Lara Cobler, Carme de Bolós. Published in: Atlas Genet Cytogenet Oncol Haematol.
TY - JOUR. T1 - Mucins in pancreatic cancer and its microenvironment. AU - Kaur, Sukwinder. AU - Kumar, Sushil. AU - Momi, Navneet. AU - Sasson, Aaron R.. AU - Batra, Surinder Kumar. PY - 2013/10/1. Y1 - 2013/10/1. N2 - Pancreatic cancer remains a lethal malignancy with poor prognosis owing to therapeutic resistance, frequent recurrence and the absence of treatment strategies that specifically target the tumour and its supporting stroma. Deregulated cell-surface proteins drive neoplastic transformations and are envisioned to mediate crosstalk between the tumour and its microenvironment. Emerging studies have elaborated on the role of mucins in diverse biological functions, including enhanced tumorigenicity, invasiveness, metastasis and drug resistance through their characteristic O-linked and N-linked oligosaccharides (glycans), extended structures and unique domains. Multiple mucin domains differentially interact and regulate different components of the tumour microenvironment. This Review ...
Mucin 1 (MUC1) is a heterodimeric protein that is aberrantly expressed by diverse human carcinomas and certain hematological malignancies (Kufe, 2009). The overexpression of MUC1, as found in human cancers, is associated with the induction of anchorage-independent growth and tumorigenicity (Li et al., 2003). Based on these findings, MUC1 has emerged as an attractive target for the development of anticancer agents. However, the identification of drugs that block MUC1 has been limited by the lack of sufficient information regarding how MUC1 contributes to the growth and survival of malignant cells. In this regard, the MUC1 protein is translated by a single mRNA and then undergoes autocleavage into two subunits that in turn form a heterodimer (Kufe, 2009). The MUC1 N-terminal subunit is the mucin component of the heterodimer that contains the characteristic glycosylated tandem repeats and is expressed on the cell surface in a complex with the MUC1 C-terminal transmembrane subunit (MUC1-C) (Kufe, ...
TFF-2, human recombinant protein, Trefoil Factor 2, Spasmolytic polypeptide (SP), Spasmolysin validated in (PBV10395r-100), Abgent
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Natural fragmentation of polypeptide chains by autoproteolysis occurs in a number of protein families. It is a vital step in the maturation of several enzymes and in the formation of membrane-associated mucins that constitute a part of the protective mucus barrier lining epithelial cells. These reactions follow similar routes involving an initial N-O or N-S acyl shift starting with a nucleophilic attack by a hydroxyl or thiol group on a carbonyl carbon followed by resolution of the ester intermediate. Previous studies indicate that distortion of the scissile peptide bond may play a role in autoproteolysis. Our structural, biochemical and molecular dynamics studies of the autoproteolyzed SEA domains from human membrane-bound mucin MUC1 and human orphan receptor GPR116 confirmed this by revealing a novel biochemical mechanism where the folding free energy accelerates cleavage by imposing conformational strain in the precursor structure. This mechanism may well be general for autoproteolysis. The ...
臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。. To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of NTU Repository with Academic Hub to form NTU Scholars.. ...
Extraction, characterization, and use of mucin biopolymers for biomedical applications.. For thousands of years, and still today, humans have exploited natural materials for their benefit. For instance, wood-extracted cellulose fibers are used to make paper and animal-extracted hyaluronic acid is used in cosmetic anti wrinkle creams. In this proposal we will develop the use of mucins, i.e. a molecules extracted from mucus, for biomedical applications. Mucins are the main components of mucus, the gel that covers the wet epithelium of all mammals. Commonly secreted by animal tissues, they can be extracted from abundant sources such as pig stomachs and bovine submaxillary glands.. The study and technological use of mucins are hampered by the poor control over the quality of the mucins available. In this proposal, we first aim to develop a quality-control benchmark by measuring a series of physical and biochemical properties. Using this benchmark, we will then identify new processes to efficiently ...
TY - JOUR. T1 - Chromosomal Localization of a Human Mucin Gene (MUC8) and Cloning of the cDNA Corresponding to the Carboxy Terminus. AU - Shankar, Viswanathan. AU - Pichan, Prabhasankar. AU - Eddy, Roger L.. AU - Tonk, Vijay. AU - Nowak, Norma. AU - Sait, Sheila N J. AU - Shows, Thomas B.. AU - Schultz, Roger E.. AU - Gotway, Garrett. AU - Elkins, Ronald C.. AU - Gilmore, Michael S.. AU - Sachdev, Goverdhan P.. PY - 1997. Y1 - 1997. N2 - A partial cDNA (pAM1) encoding a major airway mucin glycoprotein with novel tandem repetitive sequence has recently been cloned. In this article, we report additional new sequence derived by 3′-rapid amplification of cDNA ends technique. The sequence corresponds to a stop codon, 3′-untranslated region of 458 bp, a polyadenylation signal, and poly A+ tail, and represents the extreme carboxy terminus of MUC8. A plasmid construct (pAM3) in pBluescript was generated by in-frame ligation of pAM1 to the 479-bp 3′UTR of MUC8. A 5′-end 325-bp fragment of this ...
Mucin glycoproteins are secreted in large amounts by the intestinal epithelium and constitute an efficient component of innate immune defenses to promote homeostasis and protect against enteric pathogens. In this study, our objective was to investigate how the bacterial enteropathogen Shigella flexneri, which causes bacillary dysentery, copes with the mucin defense barrier. We report that upon in vitro infection of mucin-producing polarized human intestinal epithelial cells, virulent S. flexneri manipulates the secretion of gel-forming mucins. This phenomenon, which is triggered only by virulent strains, results in accumulation of mucins at the cell apical surface, leading to the appearance of a gel-like structure that favors access of bacteria to the cell surface and the subsequent invasion process. We identify MUC5AC, a gel-forming mucin, as a component of this structure. Formation of this gel does not depend on modifications of electrolyte concentrations, induction of trefoil factor expression,
1. Human gallbladder mucin has been implicated to play a role in gallstone disease. In spite of this fact relatively little is known about the structure of human gallbladder mucin. In this study we have investigated the possible heterogeneity of mucin. For this purpose polyclonal and monoclonal antibodies against gallbladder mucin were raised. All antibodies reacted primarily with carbohydrate antigenic determinants. With these antibodies the immunoreactivity of gallbladder mucin from 60 patients with cholesterol gallstones and 20 subjects without stones was screened. In addition, reactivity with several lectins was studied.. 2. Considerable heterogeneity was found with both antibody and lectin typing, but there was no significant difference in heterogeneity between mucin from patients with gallstones and control subjects. Immunoblotting revealed that there was similarity between the reaction of the polyclonal antibody and the Helix pomatia agglutinin. All mucin preparations reacting with the ...
The commensal bacteria in colon live and thrive in the outer loose mucus layer. This is possible after the Muc2 mucin network has expanded in volume, such that it allows the bacteria to penetrate into the mucin network. Once inside the mucus gel, the commensal bacteria can use its large number of glycan-degrading enzymes that release one monosaccharide at a time from the mucin glycans. In this way, it will take some time for the bacterial enzymes to reach and expose the mucin protein core for proteolysis that will degrade the mucin protein core. In this way the mucin polymeric network of the loose mucus is maintained for some time to give a relatively thick outer mucus layer. Although difficult to visualize, the outer loose mucus will probably be increasingly degraded the closer it comes to the luminal content. It is further indicated that bacteria contribute to dissolving the outer loose mucus as germ-free mice have, relative to the inner layer, a thick outer loose mucus layer (16). As ...
Purpose : Terminally differentiated stratified epithelial cells maintain barrier function at the ocular surface through a specialized protective structure composed of highly O-glycosylated transmembrane mucins. Relatively low levels of N-glycans are found on transmembrane mucins, and their structure and function remains poorly characterized. The purpose of this study was to define the contribution of mucin N-glycans to the ocular surface glycocalyxl barrier. Methods : Transmembrane mucins were purified from stratified corneal epithelial cell cultures using size exclusion chromatography and isopycnic density centrifugation. N-glycans released from mucin isolates were analyzed by liquid chromatography and electrospray ionization mass spectroscopy. N-glycosylation was inhibited using chemical, enzymatic and siRNA (MGAT1) approaches. Plasma membrane proteins were labeled by biotinylation. Lectin blot, immunoblot, and qPCR were used to determine levels of carbohydrate, protein, and mRNA, ...
BACKGROUND: Bacterial gastroenteritis causes morbidity and mortality in humans worldwide. Murine Citrobacter rodentium infection is a model for gastroenteritis caused by the human pathogens enteropathogenic Escherichia coli and enterohaemorrhagic E. coli. Mucin glycoproteins are the main component of the first barrier that bacteria encounter in the intestinal tract. METHODOLOGY/PRINCIPAL FINDINGS: Using Immunohistochemistry, we investigated intestinal expression of mucins (Alcian blue/PAS, Muc1, Muc2, Muc4, Muc5AC, Muc13 and Muc3/17) in healthy and C. rodentium infected mice. The majority of the C. rodentium infected mice developed systemic infection and colitis in the mid and distal colon by day 12. C. rodentium bound to the major secreted mucin, Muc2, in vitro, and high numbers of bacteria were found in secreted MUC2 in infected animals in vivo, indicating that mucins may limit bacterial access to the epithelial surface. In the small intestine, caecum and proximal colon, the mucin expression ...
Abstract: : Purpose: Our previous investigations proved that conjunctival impression cytology (CIC) is a valid, non-invasive technique that is useful to detect mRNA levels of ocular mucin genes in healthy individuals. This study was designed to perform the relative quantitation of MUC1, 2, 4, 5AC and 7 mucin genes in CIC samples from healthy males and females, younger and older of 60 years of age. Methods: Two polyethersulfone filters were applied to the superior bulbar conjunctiva of 38 healthy donors. Two age categories were considered: less that 60 years (7 males, 7 females), and 60 years or older (15 males, 9 females). Real time RT-PCR was performed using total RNA to examine the expression of MUC1, 2, 4, 5AC and 7. Their relative quantity was determined by SYBR® Green I dye detection. The housekeeping gene GAPDH was used to normalize the amount of expression levels. Data of mucin genes were analyzed using a logarithmic transformation to obtain a distribution closer to the normal ...
Mucin glycoproteins play a key role in the normal function of the airway epithelium. We examined the expression of mucin genes, MUC3, 4, 5AC, 5B, 6, 7, and 8 in human fetal tissues to establish the localization and age of onset of expression of each mucin gene during human development. We detected expression of MUC4, 5AC, 5B, and 7 in the mid-trimester airway epithelium but did not detect expression of MUC3, 6, or 8. MUC4 was expressed in the trachea and large airways in the majority of cells in the airway epithelium. Expression of MUC5AC was only seen in individual goblet cells in the trachea, while MUC5B was expressed in the surface epithelium of the trachea at 13 wk but was largely restricted to submucosal glands by 23 wk of gestation.
DESCRIPTION (provided by applicant): Mucin-type glycans are conjugated glycans linked through N-acetylgalactosamine at the reducing end to ser or thr in the peptide. They are found primarily in secreted mucins and membrane-bound glycoproteins, including some mucins. One major function of membrane-associated mucin-type glycans is to guide the migration of circulating leukocytes to site of injury and lymphoid tissues through interaction of sialyl Lewis x- containing glycans located at the non-reducing termini with selectins. The major function of mucin-type glycans in secreted mucins is to protect mucus-secetroy tissues by retention of water, and trapping and clearance of airborne and injected pathogens. Functions of both types of glycans are controlled to a large extent by 6GlcNAc branch structures;for membrane-associated mucin-type glycans, it is core 2 and for mucin-type glycans found in secreted mucins, it is core 2, core 4, and blood group I antigen. Core 2 can be synthesized by core 2 ...
TY - JOUR. T1 - Mucin expression profile in Barretts, dysplasia, adenocarcinoma sequence in the esophagus. AU - Burjonrappa, S. C.. AU - Reddimasu, S.. AU - Nawaz, Z.. AU - Gao, X.. AU - Sharma, Poonam K. AU - Loggie, Brian W.. PY - 2007/1/1. Y1 - 2007/1/1. N2 - BACKGROUND: The molecular events that accompany the progression to adenocarcinoma (ADC) of the esophagus are poorly understood. Aberrant mucin receptor expression can contribute to increased cell growth and metastatic ability. AIM: The aim of this study was to establish a pattern for mucin (MUC) gene expression in the esophageal mucosa under normal and pathological conditions. SETTING: University Hospital Cancer Center Laboratory. Archived tissue samples studied in a retrospective fashion. MATERIALS AND METHODS: Tissue samples were obtained from the archives of patients with histological evidence of Barretts esophagus (BE) progressing to ADC. Immunohistochemical analysis was performed using mouse monoclonal antibodies for MUC1, MUC2, ...
Snail mucin is pretty much exactly what it sounds like: it is a slimy secretion that comes from everyones favorite garden critter, snails.. According to popular K-beauty blog The Klog, snail mucin is harvested from species of edible snails like the Roman snail (the kind most often used in escargot), the garden snail, and the giant African snail. By only using snail mucin harvested from edible snails, beauty brands can ensure that the snail mucin used in their products are safe for topical application.. The snails are grown in captivity for sanitary and sustainability purposes, and snail farmers from Chile to Italy have developed ways to extract the magic goo without harming the snails. As featured in a short documentary by Great Big Story, Italian scientist and snail farmer Simone Sampo created a machine that he calls a spa for snails that helps the snails produce batches of mucin before they are re-released into Sampos garden.. It is worth noting that the snail mucin used in beauty products ...
The objective of this study was to investigate the effect of alkali treatment of mucin on the mucoadhesive properties and tablet parameters of metronidazole tablets. Mucin was extracted from the African giant snails (Archachatina maginata) by differential precipitation using acetone, air-dried and pulverized. Different portion of the mucin powder was treated with mineral bases (sodium and ammonium hydroxides) and organic base (pyridine) at high and low concentrations and at varied times of 1 and 12 h. Ten batches of metronidazole tablets (B1-B10) were prepared with the modified mucin by direct compression. Their granules and tablets were evaluated for flow properties and tablets parameters, respectively. Tablet mucoadhesion was determined using the mass flow rate method. All the batches of granules gave good flow characteristics with their angles of repose , 30°. The formulated tablets passed the weight variation test and had hardness values within the range of 3.2 to 5.0 kp and friability of ...
Genomic organization of the 3′-region of the human MUC5AC mucin gene: additional evidence for a common ancestral gene for the 11p15.5 mucin gene family ...
Changes in the expression of mucin genes in gastrointestinal cancers is thought to contribute to the development of the disease. In our laboratory we have shown previously that MUC5AC is aberrantly expressed in rectosigmoid villous adenomas. However, the regulatory mechanisms underlying that altered profile of expression is unknown. In order to study its regulation at the transcriptional level, we have isolated and characterized 5.5 kb of the 5´-flanking region of the mouse Muc5ac mucin gene. The promoter is flanked by a TATA box and a transcriptional start site is located 22 bp downstream of the TATA box. Analysis of the sequence showed a high density of binding sites for Smad4, an essential factor in the signalling cascade activated by TGF-β (transforming growth factor-β), and Sp1, an important factor in the regulation of MUC5AC. This led us to study Muc5ac regulation by TGF-β. We show that exogenous addition of TGF-β to the cells induces Muc5ac endogenous expression, promoter activity ...
TY - JOUR. T1 - Molecular basis of antibody binding to mucin glycopeptides in lung cancer. AU - Qu, Jin. AU - Yu, Hongtao. AU - Li, Fenge. AU - Zhang, Chunlei. AU - Trad, Ahmad. AU - Brooks, Cory. AU - Zhang, Bin. AU - Gong, Ting. AU - Guo, Zhi. AU - Li, Yunsen. AU - Ragupathi, Govind. AU - Lou, Yanyan. AU - Hwu, Patrick. AU - Huang, Wei. AU - Zhou, Dapeng. PY - 2016/2. Y1 - 2016/2. N2 - Glycopeptides bearing Tn epitopes are emerging targets for cancer diagnosis and immunotherapy. In this study, we analyzed membrane proteins containing O-glycosylated tandem repeat (TR) sequences in lung cancer patients of different types and stages, using gene microarray data in public domain. The expression of Tn and glycopeptide epitopes on the surface of lung cancer cell lines were studied by monoclonal IgG antibodies 14A, 16A, and B72.3. The binding of mAbs to synthetic glycopeptides were studied by surface plasmon resonance. Nine mucin mRNAs were found to be expressed in lung cancer patients but at similar ...
Mucous membranes are covered with mucus, a viscoelastic hydrogel that plays an essential role in their protection from shear and pathogens. The viscoelasticity of mucus is owing to mucins, a group of densely glycosylated proteins. Mucins can interact with a wide range of surfaces; thus, there is big interest in exploring and manipulating such interactions for biomedical applications. This thesis presents investigations of mucin interactions with hydrophobic surfaces in order to identify the key features of mucin lubricity, as well as describes the development of materials that are optimized to interact with mucins.. In Paper I we investigated the domains which make mucins outstanding boundary lubricants. The results showed that the hydrophobic terminal domains of mucins play a crucial role in the adsorption and lubrication on hydrophobic surfaces. Specifically, protease digestion of porcine gastric mucins and salivary mucins resulted in the cleavage of these domains and the loss of lubricity and ...
O-glycans are a class of glycans that modify serine or threonine residues of proteins. Biosynthesis of O-glycans starts from the transfer of N-acetylgalactosamine (GalNAc) to serine or threonine. The first GalNAc may be extended with sugars including galactose, N-acetylglucosamine, fucose, or sialic acid, but not mannose, glucose, or xylose. Depending on the sugars added, there are four common O-glycan core structures, cores 1 through 4, and an additional four, cores 5 though 8. Mucins are highly O-glycosylated glycoproteins ubiquitous in mucous secretions on cell surfaces and in body fluids. Mucin O-glycans can be branched, and many sugars or groups of sugars are antigenic. Important modifications of mucin O-glycans include O-acetylation of sialic acid and O-sulfation of galactose and N-acetylglucosamine ...
O-glycans are a class of glycans that modify serine or threonine residues of proteins. Biosynthesis of O-glycans starts from the transfer of N-acetylgalactosamine (GalNAc) to serine or threonine. The first GalNAc may be extended with sugars including galactose, N-acetylglucosamine, fucose, or sialic acid, but not mannose, glucose, or xylose. Depending on the sugars added, there are four common O-glycan core structures, cores 1 through 4, and an additional four, cores 5 though 8. Mucins are highly O-glycosylated glycoproteins ubiquitous in mucous secretions on cell surfaces and in body fluids. Mucin O-glycans can be branched, and many sugars or groups of sugars are antigenic. Important modifications of mucin O-glycans include O-acetylation of sialic acid and O-sulfation of galactose and N-acetylglucosamine ...
TY - JOUR. T1 - IL-13 induces mucin production by stimulating epidermal growth factor receptors and by activating neutrophils. AU - Shim, Jae Jeong. AU - Dabbagh, Karim. AU - Ueki, Iris F.. AU - Dao-Pick, Trang. AU - Burgel, Pierre Regis. AU - Takeyama, Kiyoshi. AU - Tam, Dominic Cheng Wei. AU - Nadel, Jay A.. PY - 2001/1. Y1 - 2001/1. N2 - Mucus hypersecretion contributes to the morbidity and mortality in acute asthma. Both T helper 2 (Th2) cytokines and epidermal growth factor receptor (EGFR) signaling have been implicated in allergen-induced goblet cell (GC) metaplasia. Present results show that a cascade of EGFR involving neutrophils is implicated in interleukin (IL)-13-induced mucin expression in GC. Treatment with a selective EGFR tyrosine kinase inhibitor prevented IL-13-induced GC metaplasia dose dependently and completely. Instillation of IL-13 also induced tumor necrosis factor-α protein expression, mainly in infiltrating neutrophils. Control airway epithelium contained few ...
TY - JOUR. T1 - Mucin staining is of limited value in addition to basic immunohistochemical analyses in the diagnostics of non-small cell lung cancer. AU - Micke, Patrick. AU - Botling, Johan. AU - Mattsson, Johanna Sofia Margareta. AU - Planck, Maria. AU - Tran, Lena. AU - Vidarsdottir, Halla. AU - Nodin, Björn. AU - Jirström, Karin. AU - Brunnström, Hans. PY - 2019/12/1. Y1 - 2019/12/1. N2 - Accurate diagnosis of histological type is important for therapy selection in lung cancer. Immunohistochemical (IHC) and histochemical stains are often used to complement morphology for definite diagnosis and are incorporated in the WHO classification. Our main aim was to compare different mucin stains and assess their value in relation to common IHC analyses in lung cancer diagnostics. Using tissue microarrays from 657 surgically treated primary lung cancers, we evaluated the mucin stains periodic acid-Schiff with diastase (PASD), alcian blue-periodic acid-Schiff (ABPAS) and mucicarmine, and compared ...
Background: Repair of bone damage represents a fundamental issue in the treatment of periodontitis. The important indicator employed to monitor the bone damage repair process is the number of osteoblast cells. Achatina Fulica snail mucin (SM) contains glycosaminoglycans which have the potential to increase their number. However, the use of SM in dentistry remains limited. Purpose: To determine and prove the effect of SM gel in increasing the number of osteoblasts in rat models suffering from periodontitis. Methods: This study used 36 rat models divided into three groups, namely; a treatment group (T: 20% snail mucin gel, n = 12), a positive-control group (P: hyaluronic acid gel, n = 12) and a negative-control group (N: CMC-Na gel, n = 12). 0.2 ml of all material was applied to a pocket by means of a tuberculin syringe once a day for 14 days. Histologic observations using Haematoxylin-Eosin staining were carried out on days 3, 5, 7 and 14. Data was analyzed by two-way ANOVA followed by a post-hoc LSD.
The present study was undertaken to analyze the role of the MUC4 mucin in pancreatic tumor development and progression. Mucins are generally known to protect surface epithelia from injuries and infections under normal physiological conditions but often display deregulated expression in malignant cells (4 , 6 , 10) . We have previously reported that the human mucin gene MUC4 is aberrantly expressed in pancreatic tumors and cell lines but has no detectable expression in the normal pancreas (10) . The present in vitro and in vivo data reveal that the silencing of MUC4 expression results in altered tumor cell behavior, decreased growth, and a marked reduction in metastatic incidences in an orthotopic mouse model of pancreatic cancer. These results are in support of the previous observations and establish a direct association of MUC4 expression with the disease development.. Down-regulating the expression is one of the convenient and reliable approaches for functional analyses of genes (reviewed in ...
MUC5AC (Mucin 5AC / Gastric Mucin) Antibody, Mouse Monoclonal Antibody [Clone MUC5AC/917 ] validated in IHC, IF, FC (AH11914-7), Abgent
The MUC-1 antigen is a membrane-anchored mucin-type glycoprotein present in malignant and normal epithelial cells of certain organs, e.g. breast, lung, ovary, pancreas and colon (1). The apoprotein of the MUC-1 mucin contains a transmembrane domain, a cytoplasmic domain, and an extracellular carbohydrate rich domain. The extracellular domain is characterized by polymorphism with respect to the number of a 20 amino acid tandem repeat (VNTR polymorphism). The CanAg CA15-3 EIA is based on two mouse monoclonal antibodies, Ma695 as catcher antibody recognizing a sialylated carbohydrate epitope expressed on the MUC-1 antigen and Ma552 as tracer antibody targeting the PDTRPAPG region of the protein core (2-5).The MUC-1 breast cancer mucin (CA15-3 antigen) is secreted from tumor cells and is a well-established serological marker for monitoring the clinical course of breast cancer patients (6). ...
This graph shows the total number of publications written about Gastric Mucins by people in this website by year, and whether Gastric Mucins was a major or minor topic of these publications ...
Trousseau described spontaneous, recurrent superficial migratory thrombophlebitis associated with occult cancers, and this was later correlated with disseminated microangiopathy (platelet-rich clots in small blood vessels). Trousseau syndrome often occurs with mucinous adenocarcinomas, which secrete abnormally glycosylated mucins and mucin fragments into the bloodstream. Since carcinoma mucins can have binding sites for selectins, we hypothesized that selectin-mucin interactions might trigger this syndrome. When highly purified, tissue-factor free carcinoma mucin preparations were intravenously injected into mice, platelet-rich microthrombi were rapidly generated. This pathology was markedly diminished in P- or L-selectin-deficient mice. Heparin (an antithrombin-potentiating agent that can also block P- and L-selectin recognition of ligands) ameliorated this platelet aggregation, but had no additional effect in P- or L-selectin-deficient mice. Inhibition of endogenous thrombin by recombinant ...
TY - JOUR. T1 - Virtual pancreatoscopy of mucin-producing pancreatic tumors. AU - Nakagohri, Toshio. AU - Jolesz, Ferenc A.. AU - Okuda, Shigeo. AU - Asano, Takehide. AU - Kenmochi, Takashi. AU - Kainuma, Osamu. AU - Tokoro, Yoshiharu. AU - Aoyama, Hiromichi. AU - Lorensen, William E.. AU - Kikinis, Ron. N1 - Funding Information: Information in this article was presented at the First International Conference on Medical Image Computing and Computer-Assisted Intervention (MIC- CAI 98), Massachusetts Institute of Technology, Cambridge, Massachusetts, October I 1-1 3, 1998. This study was supported by the Japan Association for the Advancement of Medical Equipments. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.. PY - 1998. Y1 - 1998. N2 - We used computer-based virtual endoscopy techniques as a novel approach to clarify the three-dimensional (3D) surgical anatomy of the pancreas and of mucin-producing pancreatic tumors. Thirteen cases (18 lesions) of mucin- producing pancreatic ...
Healthy individuals have few goblet cells in their airways, but in patients with hypersecretory diseases goblet-cell upregulation results in mucus hypersecretion, airway plugging, and death. Multiple stimuli produce hypersecretion via epidermal growth factor receptor (EGFR) expression and activation, causing goblet-cell metaplasia from Clara cells by a process of cell differentiation. These cells are also believed to be the cells of origin of non-small-cell lung cancer, but this occurs via cell multiplication. The mechanisms that determine which pathway is chosen are critical but largely unknown. Although no effective therapy exists for hypersecretion at present, the EGFR cascade suggests methods for effective therapeutic intervention.
TY - JOUR. T1 - Clinical presentation of mucin-secreting tumors of the pancreas. AU - Tibayan, Frederick (Fred). AU - Vierra, Mark. AU - Mindelzun, Bob. AU - Tsang, Don. AU - McClenathan, James. AU - Young, Harvey. AU - Trueblood, H. Ward. PY - 2000/5/15. Y1 - 2000/5/15. N2 - Background: Pancreatitis and jaundice secondary to ductal obstruction are common in intraductal papillary mucinous tumors (IPMT) of the pancreas. However, the incidence and severity of the complications of obstruction are not well documented. The aim of the study was to investigate the clinical presentation and outcome of 10 patients with IPMT. Methods: All cases of IPMT diagnosed between 1994 and 1999 were reviewed. Results: Four of the 10 patients developed severe acute illness with systemic complications resulting from ductal obstruction. Three suffered acute cholangitis with sepsis, and 1 developed necrotizing pancreatitis and ARDS. There was 1 postoperative death in a patient with adenocarcinoma. All other patients are ...
Experiment with focus on mucin. In 2009 Yana Znamenskaya Falk moved to Sweden for a PhD study at Malmö University. In her PhD Yana chose to focus on the effect of hydration on the structural, thermodynamic and rheological properties of biopolymer mucin, which is a main component of mucus. Mucus is a slippery secretion produced by, and covering, mucous membranes. Yana is currently working at Biofilms, Research Center for Biointerfaces, at Malmö University where she has continued her research study on mucin. In 2016 Yana participated in an experiment project within MAX4ESSFUN.. Yana explains that mucus consists primarily of water (95%), the glycoprotein mucin and other components and it can be found in all internal tracts of the body, f. ex. the mouth, nose, throat, sinuses, stomach and lungs. One of the essential functions of the mucous gel is protection of tissues against dehydration (drying). In order to fulfil this function, the mucous gel should be strongly hydrated, i.e. it should contain ...
Description: Muc1 is a heavily O-glycosylated transmembrane protein expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed in ,90% breast carcinomas and metastases. In normal mammary gland it is expressed in apical surface of glandular epithelium. In breast cancer Muc1 is overexpressed; is underglycosylated and the apical localization is lost. Muc1 is transcribed as a larger precursor which is cleaved to form a larger mucin like subunit (265-400kD) and a smaller subunit (14-28kD) noncovalently associated with each other. Transgenic Muc1 has been shown to associate with all four erbB receptors and localize with erbB1 (EGFR) in lactating glands1. Muc1 can act as a ligand for ICAM-1 on HUVEC cells; it can bind beta-catenin, GSK3beta and it associates with Grb2-SOS upon phosphorylation. Epithelial Membrane Antigen, also referred to as EMA, CA15-3, Polymorphic Epithelial Mucin, PEM, ...
We reported recently that interleukin (IL)-1β exposure resulted in a prolonged increase in MUC5AC mucin production in normal, well differentiated, human tracheobronchial epithelial (NHTBE) cell cultures, without significantly increasing MUC5AC mRNA (Am J Physiol 286:L320-L330, 2004). The goal of the present study was to elucidate the signaling pathways involved in IL-1β-induced MUC5AC production. We found that IL-1β increased cyclooxygenase-2 (COX-2) mRNA expression and prostaglandin (PG) E2 production and that the COX-2 inhibitor celecoxib suppressed IL-1β-induced MUC5AC production. Addition of exogenous PGE2 to NHTBE cultures also increased MUC5AC production and IL-1β-induced Muc5ac hypersecretion in tracheas from wild-type but not from COX-2-/- mice. NHTBE cells expressed all four E-prostanoid (EP) receptor subtypes and misoprostol, an EP2 and EP4 agonist, increased MUC5AC production, whereas sulprostone, an EP1 and EP3 agonist, did not. Furthermore, specific protein kinase A (PKA) ...
Mucin production by human colonic carcinoma cells correlates with their metastatic potential in animal models of colon cancer metastasis.: Patients with mucinou
A lambda gt11 cDNA library constructed from human tracheo-bronchial mucosa was screened with a polyclonal antiserum raised to chemically deglycosylated pronase glycopeptides from human bronchial mucins. Out of 20 positives clones, one partial cDNA clone was isolated and allowed to map a novel human …
A slimy layer of mucus serves as the first line of defense against problematic microbes like the bacterial pathogen Pseudomonas aeruginosa.. We have identified mucins, the major gel-forming components of mucus, and their complex sugar structures (glycans) as protective molecules that suppress microbial virulence traits including toxin secretion, bacterial communication, and surface attachment.. This virulence reduction is associated with less host cell killing in vitro (Figure) and less colonization of burn wounds.. Significance: By attenuating the virulence of pathogens rather than eradicating them, mucin glycans are attractive candidates for novel therapeutics.. ...
TY - JOUR. T1 - Distinctive roles of vitamin A in the regulation of mucin-like glycoprotein and keratin protein synthesis in cultured rabbit tracheal epithelial cells. AU - Wu, Reen. PY - 1982. Y1 - 1982. UR - http://www.scopus.com/inward/record.url?scp=0020465996&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0020465996&partnerID=8YFLogxK. M3 - Article. AN - SCOPUS:0020465996. VL - 95. JO - Journal of Cell Biology. JF - Journal of Cell Biology. SN - 0021-9525. IS - 2 II. ER - ...
Objectives. To characterise mucins in cancer of the colon and compare these with controls using stringent biochemical measures to avoid endogenous proteolysis. Design. Crude mucus scrapings were collected from 12 specimens obtained by colectomy. Specimens from 3 traumatic colectomies and 1 sigmoid volvulus were used as controls, and compared with 6 specimens from colons resected for adenocarcinoma and 2 irradiated colons. Subjects. The median age of the 4 female patients was 76 years (range 49 - 82 years), and of the 8 male patients 46.5 years (range 16 - 74 years). Results and conclusions. The crude mucus scrapings in the 9 specimens ranged in weight from 353 mg to 7 697 mg (median 4 928 mg). The median of purified mucin in the 9 specimens was 0.72 mg/mg wet weight of scraped material. Eight samples gave non-extractable pellet material, and were treated with DTT to reduce disulphide bonds for further analysis. One of these 8 pellets was resistant to reduction and had to be digested with papain
Acute kidney injury (AKI) arises from multiple forms of renal damage, including ischemia, toxin exposure, and urinary tract obstruction. AKI-associated inflammation exacerbates damage and can lead to development of chronic kidney disease (CKD). Kidney injury molecule-1 (KIM-1) is a mucin and immunoglobulin domain-containing transmembrane protein that is highly upregulated in the proximal tubule after kidney injury. While chronic KIM-1 expression promotes renal fibrosis and kidney failure, Li Yang, Craig Brooks, and colleagues at Harvard Medical School reveal that KIM-1 dampens early inflammatory responses and protects the kidney following injury. Mice expressing a mutant form of KIM-1 that lacks the mucin domain (KIM-1Δmucin) were more susceptible to both ischemia-induced and toxin-induced AKI compared to control animals. Loss of the mucin domain disrupted KIM-1-mediated phagocytosis in proximal tubular cells. This reduction in phagocytosis in KIM-1Δmucin mice reduced clearance of apoptotic ...
Snail Mucin (yes, snail slime) What I want to know is- Who was the first person to put this on their face? Why should YOU be interested? Below are some of the claims Ive found for snail mucin through articles, cosmetic brands & ingredient companies. However, I only found one clinical study, and the one…
Enriched with 96% snail mucin, this COSRX essence is the best thing that ever happened to your skin. Anti-aging and anti-acne Healing and revitalising For all skin types Free of parabens, alcohols or synthetic dyes COSRX Advanced Snail 96 Mucin Power Essence is a pure formula crafted from
Among several genes identified by SST-REX cloning, Endomucin is noteworthy, as it encodes a protein that belongs to the sialomucin family. CD34 is the prototypic member of this family and has been widely used as a marker of HSCs and/or progenitor cells and of endothelial cells. The highly conserved amino acid sequence, protein structure, and genomic organization of CD34, endoglycan, and podocalyxin suggest that they are closely related to each other (29). These molecules reportedly are expressed on HSCs and/or progenitor cells and on endothelial cells and are supposed to have redundant functions (29, 30). Although Endomucin exhibits a protein structure highly related to that of these three molecules, its genomic structure is distant, indicating a different phylogeny (19).. Endomucin was originally identified as an endothelium-specific protein (19), but we and others have reported possible involvement of Endomucin in HSC development in the embryo (24, 31). Identification of Endomucin on SST-REX ...
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Gastric cancer (GC) is one of the most common malignancies worldwide. Better knowledge of the changes in gene expression that occur during gastric carcinogenesis may lead to improvements in diagnosis, treatment and prevention. In this study, we screened for genes upregulated in GC by comparing gene expression profiles from microarray and serial analysis of gene expression and identified the HOXA10 gene. The aim of the present study was to investigate the significance of HOXA10 in GC. Immunohistochemical analysis demonstrated that 221 (30%) of 749 GC cases were positive for HOXA10, whereas HOXA10 was scarcely expressed in non-neoplastic gastric mucosa except in the case of intestinal metaplasia. Next, we analyzed the relationship between HOXA10 expression and clinicopathological characteristics. HOXA10 expression showed a significant inverse correlation with the depth of invasion and was observed more frequently in the differentiated type of GC than in the undifferentiated type of GC. HOXA10 ...
TY - JOUR. T1 - New trends in the study of podoplanin as a cell morphological regulator. AU - Sawa, Yoshihiko. N1 - Copyright: Copyright 2010 Elsevier B.V., All rights reserved.. PY - 2010/8. Y1 - 2010/8. N2 - Podoplanin is a mucin-type glycoprotein firstly identified in podocytes, which is homologous to the type I alveolar cell specific T1α-2 antigen and to the oncofetal antigen M2A recognized by the D2-40 antibody. Podoplanin possesses a platelet aggregation-stimulating domain causes the platelet aggregation on cancer cells by the binding activity to CLEC-2. Podoplanin also contributes to the formation of membrane-actin structures. The increased podoplanin expression is found in squamous cell carcinomas at the invasive edge. It has been reported that the podoplanin induces an actin cytoskeleton rearrangement dependent on the RhoA GTPase activation to phosphorylate ezrin and facilitates an epithelial-mesenchymal transition (EMT) which induces the single cell migration of cancer cells. However, ...
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Mucin 4 (MUC4) in samples from Tissue homogenates, cell lysates, cell culture supernates and other biological fluids. with no significant corss-reactivity with analogues from other species ...
They analysed gastrointestinal mucins initially because the oligosaccharides were similar to those of cell membranes but mucins ... In addition, mucins were an important part of cellular defences so of interest in their own right. They were able to define the ... Hounsell, E F; Feizi, T (1982). "Gastrointestinal mucins. Structures and antigenicities of their carbohydrate chains in health ... Biochemical Society Transactions 12 (4) 591-596 E F Hounsell and T Feizi (1982) Gastrointestinal mucins - structures and ...
Mucins thicken mucus, and their concentration has been found to be high in cases of chronic bronchitis, and also to correlate ... Chronic bronchitis is a respiratory disease marked by overproduction of mucus and mucins. The excess mucus is produced by ... Effective mucociliary clearance depends on airway hydration, ciliary beating, and the rates of mucin secretion. Each of these ... Voynow, J (Feb 2009). "Mucins, mucus, and sputum". Chest. 135 (2): 505-512. doi:10.1378/chest.08-0412. PMID 19201713. Vestbo, J ...
Analysis of substrates and products for four N-acetyl-D-glucosaminyl-transferases involved in mucin synthesis". Carbohydrate ... I. Detection in canine submaxillary glands of an N-acetylglucosaminyltransferase which acts on mucin substrates". The Journal ... Williams D, Longmore G, Matta KL, Schachter H (December 1980). "Mucin synthesis. II. Substrate specificity and product ... uridine diphosphoacetylglucosamine-mucin beta-(1->6)-acetylglucosaminyltransferase, core 2 acetylglucosaminyltransferase, core ...
I. Detection in canine submaxillary glands of an N-acetylglucosaminyltransferase which acts on mucin substrates". J Biol Chem. ...
One example of glycoproteins found in the body is mucins, which are secreted in the mucus of the respiratory and digestive ... The sugars when attached to mucins give them considerable water-holding capacity and also make them resistant to proteolysis by ...
Mucin: The stomach has a priority to destroy the bacteria and viruses using its highly acidic environment but also has a duty ... The way that the stomach achieves this is by secreting mucin and bicarbonate via its mucous cells, and also by having a rapid ... Mucous neck and pit cells: Produce mucin and bicarbonate to create a "neutral zone" to protect the stomach lining from the acid ...
... glycolipids and mucins in tissues. The reaction of periodic acid oxidizes the vicinal diols in these sugars, usually breaking ... which often secrete neutral mucins. Paget disease of the breast. Alveolar soft part sarcoma. Staining macrophages in Whipple's ...
Mucin 16 is a membrane associated mucin that possesses a single transmembrane domain. A unique property of MUC16 is its large ... The expression of mucin 16 has been shown to be altered in dry eye, cystic fibrosis, and several types of cancers. MUC16 (CA- ... All mucins contain a tandem repeat domain that has repeating amino acid sequences high in serine, threonine and proline. The C- ... MUC16 is a member of the mucin family glycoproteins. CA-125 has found application as a tumor marker or biomarker that may be ...
The major mucins secreted - MUC5AC, and MUC5B are large polymers that give the mucus its rheologic, or viscoelastic properties ... MUC5AC is the main gel-forming mucin secreted by goblet cells, in the form of threads, and thin sheets. MUC5B is a polymeric ... Mucins, a kind of polyelectrolyte proteoglycans, are the main component of mucus, which provides the polyelectrolyte effect in ... For instance, the charged residue on mucin is protonated at a normal pH value of the stomach, approximately pH 2. In this case ...
Mucins line the apical surface of epithelial cells in the lungs, stomach, intestines, eyes and several other organs. Mucins ... Mucin 1, cell surface associated (MUC1), also called polymorphic epithelial mucin (PEM) or epithelial membrane antigen or EMA, ... The release of mucins is performed by sheddases. The extracellular domain includes a 20 amino acid variable number tandem ... MUC1 is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein. MUC1 has a core protein mass of ...
They also secrete mucin. The intercalated ducts take the bicarbonate to intralobular ducts which become lobular ducts. These ...
nov., a new member of the family Chitinophagaceae producing mucin". Journal of Microbiology. 57 (5): 356-361. doi:10.1007/ ... Mucibacter soli produces mucin. "Genus: Mucibacter". LPSN.DSMZ.de. Kim, Min-Kyeong; Park, Sewook; Kim, Tae-Su; Joung, Yochan; ...
Naresh, Ram; Hampson, David J. (1 January 2010). "Attraction of Brachyspira pilosicoli to mucin". Microbiology (Reading, ... Ogata, Sho; Shimizu, Ken; Tominaga, Susumu; Nakanishi, Kuniaki (2017). "Immunohistochemical study of mucins in human intestinal ... mucin layers thanks to its spiroid shape and flagellum (see film ), attach to colonocytes apically and to each other laterally ... the body with mucins to invade, epithelia to attach and glucose available. Chapot F. (2020) hypothesises that Brachyspira may ...
Die Mucin-Polysaccharide der Eiweißdrüse. 1. Mitteil.: Die Identität von Galaktogen und Sinistrin. Hoppe-Seyler's Zeitschrift ...
... stains acid mucins to yellow color. It also stains mast cell granules brown. It can be used with live cells. ...
Naughton J, Duggan G, Bourke B, Clyne M (2014). "Interaction of microbes with mucus and mucins: recent developments". Gut ...
The main component of mucus is a glycoprotein called mucin and the type secreted varies according to the region involved. Mucin ... It covers the cheeks, inner surfaces of the lips, and floor of the mouth, and the mucin produced is highly protective against ... Frenkel, Erica Shapiro; Ribbeck, Katharina (2014-10-24). "Salivary Mucins Protect Surfaces from Colonization by Cariogenic ...
The target might be Mucin 5AC. The antibody was discovered by scientists at Neogenix Oncology using tumor antigens that had ...
They secrete neutral mucin that protects the esophagus from acidic gastric juices. They are simple tubular or branched tubular ... They secrete acid mucin for lubrication.[citation needed] Esophageal cardiac glands- mucous glands located near the cardiac ...
Acinar adenocarcinoma with intraluminal blue mucin. In uncertain cases, a diagnosis of malignancy can be discarded by ... Prominent nucleoli Intraluminal eosinophilic secretion Intraluminal blue mucin Adenocarcinoma with two mitoses in reactive ...
... with special emphasis on its mucins. He discovered that the mucins MUC1 and MUC-X, which are transferred to the milk fat ... Patton, Stuart (2001). "MUC1 and MUC-X, Epithelial Mucins of Breast and Milk". Bioactive Components of Human Milk. Advances in ... "Polymorphic forms of the epithelial mucin, PAS-I (MUC1), in milk of Holstein cows (Bos taurus)". Comparative Biochemistry and ...
These include inflammatory cytokines, carbonic anyhydrase, E-cadherin and mucins; however, their direct implications in LPR are ...
Occurrence, partial purification, and action on linkages in submaxillary mucins". Biochemistry. 8 (5): 2034-43. doi:10.1021/ ...
Mucin and cadherin-like protein is a protein that in humans is encoded by the MUPCDH gene. This gene is a novel mucin-like gene ... "Entrez Gene: MUCDHL mucin and cadherin-like". Yagi T, Takeichi M (2000). "Cadherin superfamily genes: functions, genomic ... While encoding nonpolymorphic tandem repeats rich in proline, serine and threonine similar to mucin proteins, the gene also ... a novel developmentally regulated protocadherin with mucin-like domains". J Biol Chem. 275 (32): 24622-9. doi:10.1074/jbc. ...
Its type strain is MucT (=ATCC BAA-835T =CIP 107961T). A. muciniphila is able to use mucin as its sole source of carbon and ... Akkermansia muciniphila is a species of human intestinal mucin-degrading bacterium, the type species for a new genus, ... Derrien, Muriel; Collado, M. Carmen; Ben-Amor, Kaouther; Salminen, Seppo; de Vos, Willem M. (2008). "The Mucin Degrader ... nov., a human intestinal mucin-degrading bacterium". International Journal of Systematic and Evolutionary Microbiology. 54 (5 ...
Goblet cells are filled with mucin (MUC). MUC5AC Mucin 5AC is a gel-like mucin product of goblet cells. Interleukin-13 induces ... Fahy JV (2002). "Goblet cell and mucin gene abnormalities in asthma". Chest. 122 (6 Suppl): 320S-326S. doi:10.1378/chest.122.6_ ...
Mucin 5AC is a useful immunohistochemistry marker. Characteristic genetic alterations are those of KRAS and GNAS. Further ... In 1982, IPMN was reported as a "mucin-producing tumor" by Kazuhiko Ohashi of the Japanese Foundation for Cancer Research. ... IPMNs are lined with mucin-secreting columnar epithelium. In most cases, IPMNs are diagnosed based on clinical and radiographic ...
Mucin-like protein 1 is a protein that in humans is encoded by the MUCL1 gene. GRCh38: Ensembl release 89: ENSG00000172551 - ... Hubé F, Mutawe M, Leygue E, Myal Y (2005). "Human small breast epithelial mucin: the promise of a new breast tumor biomarker". ... "Entrez Gene: SBEM small breast epithelial mucin". Dias Neto E, Correa RG, Verjovski-Almeida S, et al. (2000). "Shotgun ... 2006). "Identification of an octamer-binding site controlling the activity of the small breast epithelial mucin gene promoter ...
Mucins are large glycoproteins that give the mucus its gel-like properties. As mucins are lost during routine histology ... Below the mucin granules, surface mucous cells have a Golgi apparatus, the nucleus, and small amounts of rough endoplasmic ... Surface mucous cells have large quantities of mucin granules in their apical (top) surface and project short microvilli into ... These are shorter than their surface counterpart and contain lesser quantities of mucin granules in their apical surface. The ...
2004, (Type species of the genus).; New Latin neuter gender noun mucinum, mucin; New Latin adjective philus from Greek ... nov., a human intestinal mucin-degrading bacterium Jangi, Sushrut; Gandhi, Roopali; Cox, Laura M.; Li, Ning; von Glehn, Felipe ... nov., a human intestinal mucin-degrading bacterium". International Journal of Systematic and Evolutionary Microbiology. 54 (Pt ... adjective philos (φίλος) meaning friend, loving; New Latin feminine gender adjective muciniphila, mucin-loving.) However, de ...
Identification of trans Protein QTL for Secreted Airway Mucins in Mice and a Causal Role for Bpifb1.. Donoghue LJ1,2, Livraghi- ... The mucin proteins MUC5AC and MUC5B are the major glycoprotein components of mucus and have critical roles in airway defense. ... To identify genes that underlie variation in airway mucin levels, we performed genetic analyses in founder strains and ... Identification of trans Protein QTL for Secreted Airway Mucins in Mice and a Causal Role for Bpifb1 ...
The vast majority of mucin glycosylation is constituted by O... ... MUC Mucins (MUC) are high-molecular-weight glycoproteins ... There are two main classes of mucins: membrane-bound mucins, contributing to the composition of the cellular glycocalyx, and ... of the mucin molecular mass is composed of carbohydrates. The primary functions of mucins are to hydrate, protect, and ... Mucins share a common structural feature which is a tandem repeatdomain comprising a variable number of tandem repeats (VNTRs) ...
Mucin genes encode mucin monomers that are synthesized as rod-shape apomucin cores that are post-translationally modified by ... Upon stimulation, MARCKS (myristylated alanine-rich C kinase substrate) protein coordinates the secretion of mucin from mucin- ... Mucins (/ˈmjuːsɪn/) are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by ... Mature mucins are composed of two distinct regions: *The amino- and carboxy-terminal regions are very lightly glycosylated, but ...
Mucin (sv); mucin (nn); מוצין (he); Mucin (nl); Mucin (sh); Муцин (uk); Mucyny (pl); 뮤신 (ko); Mucina (gl); ميوسين (ar); Mucin ( ... mucina (it); Mucine (fr); Mutsiinid (et); Mucin (sr-el); Муцин (sr-ec); Մուցիններ (hy); mucina (ca); mucin (en); Mucine (de); ... Mucin (de); Mutsiin (et); mucins (en); Mucyna (pl); 黏蛋白 (zh); Mucini (sr) ... File nella categoria "Mucins". Questa categoria contiene 14 file, indicati di seguito, su un totale di 14. ...
Mucins in gastrointestinal cancers.. Turner MS1, McKolanis JR, Ramanathan RK, Whitcomb DC, Finn OJ. ... The mucin family has been under study by molecular biologists, biochemists, pathologists and immunologists interested in cancer ... For example, specific knowledge of cancer-associated changes in the expression and glycosylation of various mucins, which can ... Similarly, many more opportunities exist for using mucin-based therapies than are currently being considered in the clinic. ...
... to weigh in on the benefits of snail mucin for skin and polled them on their recommendations for the best snail mucin products ... Snail mucin was a gateway into K-beauty for our beauty writer Rio Viera-Newton, whose first Google doc included a Cosrx snail- ... A fixture among Rios skin-care stories is this Cosrx snail-mucin essence, which she says is a cheaper alternative to SK-II. ... Most of the snail mucin used for skin care involves the Cryptomphalus aspersa species, a.k.a. the common garden snail. Their ...
Gel-Forming Mucins in Nonvertebrate Chordates.. We identified mucins and mucin-related proteins in a variety of vertebrates as ... Other proteins with shorter mucin domains, like IgA and several receptors, are not called mucins. The mucin domains often ... "NIDO-AMOP-VWD mucins" (or "MUC4′") for these categories of mucins that previously were listed under transmembrane mucins. ... The Muc4 mucins form a group distinct from the other VWD-containing mucins, because they do not contain cysteines and have NIDO ...
Mucin-20 (MUC20) was identifiied as a gene that is up-regulated in the renal tissues of patients with immunoglobulin A ... Molecular cloning, genomic structure, and expression analysis of MUC20, a novel mucin protein, up-regulated in injured kidney. ...
... or non mucin-secreting cells [PMID: 15190477]. There are two main mucin subfamilies: secreted mucins or gel-forming mucins ( ... Mucin-2 (MUC2) belongs to the mucin family. They are high molecular weight proteins that are components of mucus and are ... MUC genes: mucin or not mucin? That is the question].. Med Sci (Paris) 20 569-74 2004 ... MUC genes: mucin or not mucin? That is the question].. Med Sci (Paris) 20 569-74 2004 ...
Mucins belong to an ever increasing family of O-glycoproteins. Secreted mucins are the main component of mucus protecting the ... Moreover, we will discuss the recent advances about the biology of both secreted and membrane-bound mucins and their key roles ... In this review, we will focus on the altered expression pattern of mucins in pancreatic cancer, from the early neoplastic ... Finally, we will discuss exciting opportunities that mucins offer as potential therapeutic targets in pancreatic cancer. ...
Mucin 4 (MUC 4) is a mucin protein that in humans is encoded by the MUC4 gene. Like other mucins, MUC-4 is a high-molecular ... As a mucin, MUC-4 also alters adhesive properties of the cell. When overexpressed, the disorganization of mucins may reduce ... "Entrez Gene: MUC-4 mucin 4, cell surface associated". "MUC4 (mucin 4, cell surface associated)". Atlas of Genetics and ... MUC-4 mucins consist of a large extracellular alpha subunit that is heavily glycosylated and a beta subunit that is anchored in ...
... mucins in the small intestine of the CF mice compared with wild-type mice. Mucins from the large intestine of both wild-type ... Intestinal mucins from cystic fibrosis mice show increased fucosylation due to an induced Fucalpha1-2 glycosyltransferase.. ... In contrast, the insoluble and soluble mucins of the small intestine in CF mice revealed a large increase in fucose, whereas ... The amino acid composition was typical of that for mucins and showed increased amounts of the insoluble (2.5-fold increase) and ...
UNC School of Medicine researchers have provided the first quantitative evidence that mucins -- the protein framework of mucus ... This is where mucins come into play. Mucins give mucus its gel-like thickness and elasticity. "Without mucins, mucus would have ... And so it looked as if there were fewer mucins. "But by using more accurate methods, we clearly saw the increase of mucins. In ... increased-mucins-pinned-to-worsening-cystic-fibrosis-symptoms More in Medicine & Health. * Newborn babies who suffered stroke ...
JOINT synovial fluid is considered to be a dialysate of blood with added mucin, which consists of hyaluronic acid1. The source ... The Stainable Mucin of Joint Tissues and Synovial Fluid. *B. F. MARTIN1. & ... JOINT synovial fluid is considered to be a dialysate of blood with added mucin, which consists of hyaluronic acid1. The source ... MARTIN, B., SHAW, N. The Stainable Mucin of Joint Tissues and Synovial Fluid. Nature 190, 835-836 (1961). https://doi.org/ ...
... Hans-Peter Hauber,1,2 Susan C Foley,1 and Qutayba Hamid1 ... Hans-Peter Hauber, Susan C Foley, and Qutayba Hamid, "Mucin Overproduction in Chronic Inflammatory Lung Disease," Canadian ...
The mucus layer is mainly comprised by large glycoproteins called mucins. Our goal is to develop methods to improve the mucins ... The mechanisms for the functioning of the mucin-based defense system are likely to be similar for many of the disease-producing ... By extension, it is therefore likely that treatment strategies based on the bodys own mucin defense could be effective both in ... The mucus layer is mainly comprised by large glycoproteins called mucins. Our goal is to develop methods to improve the mucins ...
... up-regulate mucin expression and secretion. Recently, downstream signaling causing mucin MUC2 expression induced by Pseudomonas ... Epidermal growth factor system regulates mucin production in airways. Kiyoshi Takeyama, Karim Dabbagh, Heung-Man Lee, Carlos ... Epidermal growth factor system regulates mucin production in airways. Kiyoshi Takeyama, Karim Dabbagh, Heung-Man Lee, Carlos ... We investigated the role of EGF-R and its ligands in mucin production in airway epithelium by using NCI-H292 cells (23) and by ...
... Peter L. Devine,1 Rachel J. Quin,1 Paul W. Shield,2 Yew Wah Liew,3 ... "Monoclonal Antibody Recognizing a Core Epitope on Mucin," Disease Markers, vol. 14, no. 2, pp. 99-112, 1998. https://doi.org/ ...
Frequently in tumors of the pancreas there are changes in the structure of mucin carbohydrates and/or levels of apomucin types ... Mucins are large glycoproteins that form a protective layer along the lumens of the organs of the gastrointestinal and ... Mucins in the diagnosis and therapy of pancreatic cancer Curr Pharm Des. 2000 Dec;6(18):1881-96. doi: 10.2174/1381612003398609 ... The carbohydrates of mucins on the surface of tumor cells have been reported to inhibit cells of the immune system. These ...
In, Mucins: Methods and Protocols expert researchers in the field detail many of the ... Epithelial mucins are large complex cell surface and secreted glycoproteins produced by mucosal epithelial cells. ... Mucin Methods: Genes Encoding Mucins and Their Genetic Variation with a Focus on Gel-Forming Mucins ... Authoritative and practical, Mucins: Methods and Protocols is designed to be a useful resource for those entering the mucin ...
... used options and get the best deals for COSRX Advanced Snail 96 Mucin Power Essence - 100ml at the best online prices at eBay! ... item 5 COSRX Advanced Snail 96 Mucin Power Essence 100ml 5 -. COSRX Advanced Snail 96 Mucin Power Essence 100ml. $16.39. ... item 7 [ COSRX ] Advanced Snail 96 Mucin Power Essence 100ml 7 -. [ COSRX ] Advanced Snail 96 Mucin Power Essence 100ml. $16.80 ... item 6 Cosrx Advanced Snail 96 Mucin Power Essence 100ml US Seller Free Ship EXP 2022 6 -. Cosrx Advanced Snail 96 Mucin Power ...
Repeat Mucin Ball Former Non-repeat Mucin Ball Former Arm/Group Description: Subjects that were classified as be... Subjects ... Repeat Mucin Ball Former Non-repeat Mucin Ball Former Arm/Group Description: Subjects that were classified as be... Subjects ... Subjects that were classified as being repeat Mucin Ball former. Subjects that were classified as Non-repeat Mucin Ball Former ... Szczotka-Flynn LB, Jiang Y, Stiegemeier MJ, Mutti DO, Walline J, Wilson T, Debanne S; Mucin Ball Study Group. Mucin Balls ...
Mucin also lubricates the tissues and teeth in the mouth and protects them against pathogens and... ... A critical function of mucin in saliva is to protect the mouth from drying out. ... There are two forms of salivary mucins: high or low molecular weight mucins. Low molecular weight mucin is found more often in ... Mucin is a glycosated protein, which means that it is coated with carbohydrates. This coating allows mucin to hold on to water ...
In this study, we liberated and purified O-linked glycans from porcine gastric mucin and assessed their efficacy in shaping the ... Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes ... We found that porcine mucin glycans (PMGs) and HMOs enrich for taxonomically similar resident microbes. We demonstrate that ... 3c) harbor very few mucin-degrading CGCs (Fig. S7).. Fig. 5: Prevalent gut commensals harbor high numbers of mucin-degrading ...
The O-glycan structures present in mucin are diverse and complex, consisting predominantly of core 1-4 mucin-type O-glycans ... Mucin glycan foraging in the human gut microbiome Front Genet. 2015 Mar 19;6:81. doi: 10.3389/fgene.2015.00081. eCollection ... Due to their proximity to the immune system, mucin-degrading bacteria are in a prime location to influence the host response. ... The ability to metabolize these mucin O-linked oligosaccharides is likely to be a key factor in determining which bacterial ...
Rabbit polyclonal Mucin 5AC antibody. Validated in IHC, ICC/IF and tested in Human. Cited in 4 publication(s). Independently ... Mucins are high molecular weight glycoproteins with 80% carbohydrates and 20% core protein. Gastric Mucin 5AC antigen is found ... Anti-Mucin 5AC antibody [EPR16904] (ab198294) *Research with confidence - consistent and reproducible results with every batch ... IHC image of Mucin 5AC staining in Human normal stomach formalin fixed paraffin embedded tissue section, performed on a Leica ...
The purpose of this study is to determine if the formation of mucin balls is a marker for protection from inflammatory events ... The percentage of Subjects that experienced Corneal Inflammatory Events within their Mucin Ball classification. ... The percentage of Subjects that experienced Corneal Inflammatory Events within their Mucin Ball classification. ... to the cornea and if mucin balls play a role in protection during extended contact lens wear. ...
Mucin-4. Mucin-4, MUC-4 (Ascites sialoglycoprotein, ASGP) (Pancreatic adenocarcinoma mucin) (Testis mucin) (Tracheobronchial ... mucin) [Cleaved into: Mucin-4 alpha chain (Ascites sialoglycoprotein 1, ASGP-1) ; Mucin-4 beta chain (Ascites sialoglycoprotein ... Mucin-4Imported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p>,a href="/ ... tr,A0A0G2JRU8,A0A0G2JRU8_HUMAN Mucin-4 OS=Homo sapiens OX=9606 GN=MUC4 PE=1 SV=1 ...
Mucin-4. Mucin-4, MUC-4 (Ascites sialoglycoprotein, ASGP) (Pancreatic adenocarcinoma mucin) (Testis mucin) [Cleaved into: Mucin ... Mucin-4Imported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p>,a href="/ ... R-MMU-913709 O-linked glycosylation of mucins. R-MMU-977068 Termination of O-glycan biosynthesis. ... R-MMU-913709 O-linked glycosylation of mucins. R-MMU-977068 Termination of O-glycan biosynthesis. ...
  • The mucin proteins MUC5AC and MUC5B are the major glycoprotein components of mucus and have critical roles in airway defense. (nih.gov)
  • Although some mucins are membrane -bound due to the presence of a hydrophobic membrane-spanning domain that favors retention in the plasma membrane , most mucins are secreted as principal components of mucus by mucous membranes or are secreted to become a component of saliva . (wikipedia.org)
  • All mucosal membranes of the body are covered by mucus, largely made up of the family of large glycoproteins called mucins. (pnas.org)
  • In insects, the peritrophic matrix of the gut has a protective role similar to that of the mucus of higher animals, but the insect matrix is made up of chitin and a number of chitin-binding proteins, of which some also contain mucin domains ( 6 ). (pnas.org)
  • They are high molecular weight proteins that are components of mucus and are synthesised and secreted by specialised cells of the epithelium (goblet cells, cells of mucous glands) or non mucin-secreting cells [ PMID: 15190477 ]. (ebi.ac.uk)
  • Proteomic analyses of the two mucus layers of the colon barrier reveal that their main component, the Muc2 mucin, is strongly bound to the Fcgbp protein. (ebi.ac.uk)
  • The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. (wikipedia.org)
  • Secreted mucins are the main component of mucus protecting the epithelia whereas membrane-bound mucins are thought to play important biological roles in cell-cell and cell-matrix interactions, in cell signaling and in modulating biological properties of cancer cells. (mdpi.com)
  • To explore the mucus composition, mucins insoluble and soluble in 6 M guanidinium chloride were purified by three rounds of isopycnic ultracentrifugation from the small and large intestines of CF mice (Cftr(m1UNC)/Cftr(m1UNC)) and compared with wild-type mice. (gu.se)
  • This image shows mucins, large proteins that form a network through bonds to give mucus its thickness and elasticity. (eurekalert.org)
  • CHAPEL HILL, N.C. - UNC School of Medicine researchers have provided the first quantitative evidence that mucins - the protein framework of mucus - are significantly increased in cystic fibrosis patients and play a major role in failing lung function. (eurekalert.org)
  • The research, published today in the Journal of Clinical Investigation , shows that a three-fold increase of mucins dramatically increases the water-draining power of the mucus layer. (eurekalert.org)
  • Our finding suggests that diluting the concentration of mucins in CF mucus is a key to better treatments," said Mehmet Kesimer, PhD, associate professor of pathology and laboratory medicine and co-senior author of the JCI paper. (eurekalert.org)
  • Also, by measuring mucin concentration in patient mucus, doctors could learn whether therapies are working and to what degree. (eurekalert.org)
  • Mucins give mucus its gel-like thickness and elasticity. (eurekalert.org)
  • Without mucins, mucus would have the viscosity of blood," Kesimer said. (eurekalert.org)
  • The vast majority of mucus is water, but 30 to 35 percent of the remaining solid material is made up of mucins. (eurekalert.org)
  • They set out to conduct various novel experiments to physically measure the amount of mucins in CF secretions and normal mucus. (eurekalert.org)
  • The mucus layer is mainly comprised by large glycoproteins called mucins. (gu.se)
  • To protect the epithelial cells, the mucosal surfaces are covered by a layer of secreted mucins that have a decoy/cleaning function, and underneath the mucus layer the cell-surface mucins are a dominant feature of the apical surface of mucosal epithelial cells. (gu.se)
  • We are interested in the role of mucus in veterinary (salmonids (fish) and pigs) and human medicine, as mucins and mucus have potential both to aid in earlier healing of infection and healing of chronic infections and to limit spreading of infection between individuals. (gu.se)
  • Secreted by host goblet cells, mucus is largely composed of highly glycosylated mucin proteins. (nature.com)
  • Gastric Mucin 5AC antigen is found in columnar mucus cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon but not in normal colon. (abcam.com)
  • Goblet cells reside throughout the gastrointestinal (GI) tract and are responsible for the production and preservation of a protective mucus blanket by synthesizing and secreting high molecular weight glycoproteins known as mucins. (mdpi.com)
  • The concept of the mucus layer functioning as a dynamic protective barrier is suggested by studies showing changes in mucins in inflammatory conditions of the GI tract, by the altered goblet cell response in germ-free animals, and by the enhanced mucus secretion seen in response to infections. (mdpi.com)
  • The mucin-containing mucus layer coating the GI epithelium is the front line of innate host defense. (mdpi.com)
  • The mucus lining the stomachs of pigs may well be a much awaited, copious source of 'mucins' being regarded for use as wide-ranging anti-viral agents to supplement baby formula and for use in personal hygiene and other consumer products to protect against a range of viral infections, report scientists. (medindia.net)
  • That has led to consideration of mucin, the main component of mucus, for use as an anti-viral agent in a variety of products. (medindia.net)
  • Mucins are high molar mass glycoproteins that assume an extended conformation and can assemble into mucus hydrogels that protect our mucosal epithelium. (diva-portal.org)
  • In nature, the challenging task of generating a mucus layer, several hundreds of micrometers in thickness, from micrometer-sized cells is elegantly solved by the condensation of mucins inside vesicles and their on-demand release from the cells where they suddenly expand to form the extracellular mucus hydrogel. (diva-portal.org)
  • We have identified mucins, the major gel-forming components of mucus, and their complex sugar structures (glycans) as protective molecules that suppress microbial virulence traits including toxin secretion, bacterial communication, and surface attachment. (mrsec.org)
  • Nevertheless, one of the major components of mucus is mucins. (biology-online.org)
  • This mechanism of lung defence is reliant upon a flowing mucus gel, and the major macromolecular components that determine the rheological properties of mucus are the polymeric mucins, MUC5AC and MUC5B. (biochemsoctrans.org)
  • Mucus with abnormal biophysical properties is a feature of muco-obstructive respiratory disease and can result from many different mechanisms including alterations in mucin polymer assembly, mucin concentration and the macromolecular form in mucus, as well as changes in airway surface hydration, pH and ion composition. (biochemsoctrans.org)
  • the major macromolecular components of mucus are the O-linked glycoproteins, mucins. (biochemsoctrans.org)
  • whereas, the polymeric mucins, which are the major focus of this brief review, underpin the structure and organisation of the airways mucus gel and dictate its viscoelastic properties. (biochemsoctrans.org)
  • Secreted mucins, produced by goblet cells and the gastric mucosa, provide the scaffold for a micrometers-thick mucus layer that lines the epithelia of the gut and respiratory tract 3,4 . (jove.com)
  • In addition to mucins, mucus layers also contain antimicrobial peptides, cytokines, and immunoglobulins 5-9 . (jove.com)
  • As such, the mucus is subject to numerous interactions with microbes, both pathogens and symbionts, and secreted mucins form an important interface for these interactions. (jove.com)
  • Therefore this approach is sub-optimal for studies focusing on the nature and distribution of mucins and mucus in vivo . (jove.com)
  • This allows for better preservation of the hydrated mucus layer, and thus permits the study of the numerous roles of mucins in epithelial biology. (jove.com)
  • The principal "non-water" component of mucus is mucin, a macromolecular glycoprotein largely responsible for the viscoelastic gel nature of mucus. (bl.uk)
  • Mucus barrier, mucins and gut microbiota: the expected slimy partners? (onmedica.com)
  • Mucin 3 is a major com-ponent of various mucus gels and is broadly expressed in normal and tumor cells. (scbt.com)
  • Intestinal mucin, a family of glycoproteins secreted by goblet cells, is the main constituent of the mucus protecting the gastrointestinal tract. (europa.eu)
  • The effects of sodium butyrate and sodium bromo-octanoate (an inhibitor of beta oxidation) on colonic mucus glycoprotein (mucin) synthesis have been assessed using tissue from colonic resection samples. (bmj.com)
  • 1993. Additionally we are shipping Mucin 6, Oligomeric Mucus/gel-Forming Antibodies (99) and Mucin 6, Oligomeric Mucus/gel-Forming Proteins (2) and many more products for this protein. (antibodies-online.com)
  • MUC5AC (gastric mucin) belongs to the gel-forming mucin family of glycoproteins, the major components of the protective mucus layer on the mucosal surfaces of epithelial tissues. (novusbio.com)
  • Mucin 5B Antibody (5B#19-2E) is a high quality monoclonal Mucin 5B antibody (also designated mucin 5B oligomeric mucus/gel-forming antibody or MUC5B antibody) suitable for the detection of the Mucin 5B protein of mouse, rat and human origin. (scbt.com)
  • In this work we exploit mucin glycoproteins, the gel-forming building blocks of natural mucus to deliver both hydrophobic and hydrophilic drugs. (prolibraries.com)
  • To address this challenge, the intrinsic bioactivity of the mucin biopolymer, a heavily glycosylated protein that forms the protective mucus gel covering mucosal epithelia, is leveraged. (diva-portal.org)
  • Eosinophil recruitment and mucus hypersecretion are characteristic of asthmatic airway inflammation, but eosinophils have not been shown to induce mucin production. (jimmunol.org)
  • Mucins (MUC) are high-molecular-weight glycoproteins constituted by a protein backbone (apomucin) and carbohydrate side chains. (springer.com)
  • Their mucin contains ingredients like glycoproteins, hyaluronic acid, and glycolic acid (which all have long-documented skin benefits), though snail mucin itself has not been the subject of rigorous studies. (nymag.com)
  • Mucins belong to an ever increasing family of O -glycoproteins. (mdpi.com)
  • Mucins are large glycoproteins that form a protective layer along the lumens of the organs of the gastrointestinal and reproductive tracts. (nih.gov)
  • Epithelial mucins are large complex cell surface and secreted glycoproteins produced by mucosal epithelial cells. (springer.com)
  • Mucins are high molecular weight glycoproteins with 80% carbohydrates and 20% core protein. (abcam.com)
  • Mucins are likely to be the first molecules that invading pathogens interact with at the cell surface and thus, can limit binding to other glycoproteins and neutralize the pathogen. (mdpi.com)
  • Mucins are highly O-glycosylated glycoproteins ubiquitous in mucous secretions on cell surfaces and in body fluids. (genome.jp)
  • This is because many antibodies selected for epithelial or tumor specificity have been found to react with high molecular weight glycoproteins which are produced by simple epithelial cells and have the properties of mucins. (springer.com)
  • S. J. Gendler, J. M Burchell, T. Duhig, D. Lamport, R. White, M. Parker and J. Taylor-Papadimitriou, Cloning of partial cDNA encoding differentiation and tumor-associated mucin glycoproteins expressed by human mammary epithelium, Proc. (springer.com)
  • M. Shimizu and K. Yamauchi, Isolation and characterisation of mucin-like glycoproteins in human milk fat globule membranes. (springer.com)
  • 25]G.J. Strous & J. Dekker, Mucin-type glycoproteins,Critical reviews in biochemistry and molecular biology27, 1992, 57 - 92. (actapress.com)
  • Mucins are complex and heavily glycosylated O -linked glycoproteins, which contain more than 70% carbohydrate by weight 1-3 . (jove.com)
  • Mucins are a family of glycoproteins form a protective gel layer on the surface of the mucosa under normal circumstances. (spandidos-publications.com)
  • Mucins are a group of high molecular weight glycoproteins consisting of a mucin core protein and O-linked carbohydrates. (scbt.com)
  • Cloning of partial cDNA encoding differentiation and tumor-associated mucin glycoproteins expressed by human mammary epithelium", Proc. (freepatentsonline.com)
  • Mucin-like glycoproteins, such as heparan sulfate, serve as ligands for the leukocyte adhesion molecule called CD44. (humpath.com)
  • In this study, we liberated and purified O -linked glycans from porcine gastric mucin and assessed their efficacy in shaping the recovery of a perturbed microbiota in a mouse model. (nature.com)
  • Native mucin extract from normal gastric mucosa and from gastric diffuse carcinoma. (abcam.com)
  • Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric mucin in the adult colon or due to changes in the glycosylation pattern of mucin, which expose the hidden Mucin 5AC antigen. (abcam.com)
  • We envision porcine gastric mucins to be promising antiviral components for future biomedical applications," the report says. (medindia.net)
  • A gel-forming mucin that is predominantly associated with the gastric epithelium. (jove.com)
  • 20] S. Lee, M. Müller, K. Rezwan, & N.D. Spencer,Porcine Gastric Mucin (PGM) at the Water/Poly(dimethylsiloxane) (PDMS) Interface: Influence ofpH and ionic strength on its conformation, adsorption, andaqueous lubrication properties, Langmuir 21, 2005, 8344 -8353. (actapress.com)
  • Lysozyme and mucins in gastric adenomas. (bmj.com)
  • A method for the simultaneous demonstration of lysozyme and mucins in 39 cases of gastric adenomas differentiated two intermediate cell types. (bmj.com)
  • This MAb recognizes the peptide core of gastric mucin M1 (>1,000kDa) (recently identified as Mucin 5AC). (novusbio.com)
  • Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. (novusbio.com)
  • Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins. (novusbio.com)
  • gastric mucin manufacturers and suppliers with contacts and product range are mentioned in the study. (marketpublishers.com)
  • Furthermore, gastric mucin prices in regional markets can be found in the report with regards to countries and companies. (marketpublishers.com)
  • The report also focuses on gastric mucin consumers by providing data on companies that use it. (marketpublishers.com)
  • Gastric mucin (CAS 84082-64-4) Market Research Report 2018 contents were prepared and placed on the website in February, 2018. (marketpublishers.com)
  • Please note that Gastric mucin (CAS 84082-64-4) Market Research Report 2018 is a half ready publication and contents are subject to change. (marketpublishers.com)
  • This thesis focussed upon the mucin binding of a panel of epithelial sodium channel (ENaC) blockers that have been studied as experimental therapeutic agents for cystic fibrosis (CF). Using porcine gastric mucin (PGM) as a model system the directional transport of FL-Na, FITC dextran probes (4-70 kDa) and two ENaC blockers was determined using Franz diffusion cells. (bl.uk)
  • We obtained seven monoclonal antibodies (MAb) against a gastric mucin of an ALe b patient. (aacrjournals.org)
  • Our results therefore suggest that the expression of M1 epitopes in precancerous colonic mucosae cannot be due exclusively to alterations in mucin glycosylation but may be related to the reexpression of antigens associated with native gastric mucin which is normally produced by the fetal colon during the sixth month of gestation. (aacrjournals.org)
  • The MUC6 gene encodes gastric mucin, a secreted glycoprotein that plays an essential role in epithelial cytoprotection from acid, proteases, pathogenic microorganisms, and mechanical trauma in the gastrointestinal tract (summary by Toribara et al. (antibodies-online.com)
  • Mucin 5B is abnormally expressed in gastric carcinomatous tissues. (scbt.com)
  • Upon stimulation, MARCKS (myristylated alanine-rich C kinase substrate) protein coordinates the secretion of mucin from mucin-filled vesicles within the specialized epithelial cells. (wikipedia.org)
  • Cosrx uses a similar process , as does Mizon, which - their rep tells me - heats their mesh nets to increase humidity for a terrarium effect (it ramps up mucin secretion). (nymag.com)
  • The aim of this study was to evaluate the effect of gefarnate on mucin-like glycoprotein secretion in isolated rabbit conjunctival tissue, and on corneal epithelial damage in rabbit and cat dry-eye models. (dovepress.com)
  • Gefarnate stimulated mucin-like glycoprotein secretion in conjunctival tissue in a dose-dependent manner. (dovepress.com)
  • These results suggest that gefarnate stimulates in vitro secretion of mucin-like glycoprotein in conjunctival tissue and ameliorates corneal epithelial damage in animal dry-eye models. (dovepress.com)
  • Interestingly, the absence of mucin resulted in the upregulation of 79 genes encoding secreted protein candidates, including Amuc-1100 as well as members of major protein secretion systems. (frontiersin.org)
  • Some of these gut microbes degrade mucin glycans via secretion of glycosyl hydrolases, and the resulting monosaccharides are used as additional energy and carbon sources. (frontiersin.org)
  • In airway lumens, staining for mucins was also increased in cystic fibrosis, indicating increased mucin secretion. (bmj.com)
  • Recruited neutrophils may be involved in mucin secretion in the plugs. (bmj.com)
  • Dual differentiation is thought to be responsible for combined mucin secretion and hormone production in this type of neoplasm. (bmj.com)
  • Like other mucins, MUC-4 is a high-molecular weight glycoprotein. (wikipedia.org)
  • Among the growth factors, a possible candidate is epidermal growth factor (EGF) and its receptor (EGF-R). EGF-R, a 170-kDa membrane glycoprotein, is expressed on the surface of various cells, and may be related to mucin production in stomach ( 7 ), urothelium ( 8 ), and other epithelia ( 9 ). (pnas.org)
  • Mucin-like glycoprotein was detected in the culture supernatant by an enzyme-linked lectin assay. (dovepress.com)
  • Aberrant mucin glycoprotein patterns of chronic rhinosinusitis patients with bacterial biofilms. (biomedsearch.com)
  • 4) Mucin glycoprotein MUC7 that has known bactericidal activity displays an overall reduced terminal sugar profile in biofilm positive CRS patients. (biomedsearch.com)
  • The human mucin 1 (MUC1)-type glycoprotein is expressed on the apical borders of normal secretory mammary epithelial cells ( 1 ). (aacrjournals.org)
  • This antibody recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). (biotium.com)
  • Both are recombinant mucin-type fusion proteins, produced by fusing the glycosylated mucin part of P-selectin glycoprotein ligand-1 (PSLG-1) to the Fc part of a mouse immunoglobulin in two different cells. (diva-portal.org)
  • The vast majority of mucin glycosylation is constituted by O -glycans which are linked to serine (Ser) or threonine (Thr) residues on the protein backbone, and, in fact, more than 50% of the mucin molecular mass is composed of carbohydrates. (springer.com)
  • Mucin genes encode mucin monomers that are synthesized as rod-shape apomucin cores that are post-translationally modified by exceptionally abundant glycosylation . (wikipedia.org)
  • For example, specific knowledge of cancer-associated changes in the expression and glycosylation of various mucins, which can aid in the diagnosis as well as prognosis of GI cancers, has not yet led to the use of a panel of anti-mucin antibodies as a standard diagnostic tool. (nih.gov)
  • MUC-4 belongs to the human mucin family that is membrane-anchored and can range in molecular weight from 550 to 930 kDa for the actual protein, and up to 4,650 kDa with glycosylation. (wikipedia.org)
  • We investigate the role of mucins during host defense against bacteria, as well as the biological function of changes in mucosal mucin expression and glycosylation associated with disease. (gu.se)
  • Using this method, heterogeneous glycosylation of mucins and mucin-type molecules isolated by SDS-AgPAGE and SDS-UAgPAGE was shown to consist of more than 80 different components in a single band, and in the extreme cases, up to 300-500 components (MUC5B/AC from saliva and sputum and). (gu.se)
  • MUC13 is a membrane bound mucin exhibiting abundant O- and N-glycosylation. (atlasgeneticsoncology.org)
  • O-glycosylation with complex oligosaccharides is crucial to mucin structure and function. (atlasgeneticsoncology.org)
  • The central mucin domain is dominated by proline-, serine- and threonine-rich repetitive and non-repetitive sequences that undergo O-glycosylation and account for up to 80% of the mucin molecular mass [ 2 , 9 ]. (biochemsoctrans.org)
  • While sharing common glycans, MUC5AC is more fucosylated, whereas MUC5B is more sialylated [ 10 ], and the extensive O-glycosylation results in a stiffened mucin backbone, important for gel formation [ 11 ]. (biochemsoctrans.org)
  • The mucin glycans also play a central role in host-pathogen interactions, and alteration in the pattern of mucin glycans may lead to bacterial colonisation and infection, although there are conflicting reports regarding mucin glycosylation and bacterial interactions [ 9 , 12 - 15 ]. (biochemsoctrans.org)
  • Alterations in mucin expression and glycosylation are associated with cancer development. (sigmaaldrich.com)
  • These results suggest that the mucCEST MRI signal can be used as a label-free surrogate marker to non-invasively assess mucin glycosylation and tumour malignancy. (sigmaaldrich.com)
  • Glycosylation-dependent cell adhesion molecule 1 (GlyCAM 1) mucin is expressed by lactating mammary gland epithelial cells and is present in milk. (jci.org)
  • We discuss our results in terms of the differences in the glycosylation composition of these two mucins. (diva-portal.org)
  • Mucins ( / ˈ m juː s ɪ n / ) are a family of high molecular weight , heavily glycosylated proteins ( glycoconjugates ) produced by epithelial tissues in most animals . (wikipedia.org)
  • Overexpression of the mucin proteins, especially MUC1 , is associated with many types of cancer. (wikipedia.org)
  • [9] [10] In the mouth, mucins can also recruit anti-microbial proteins such as statherins and histatine 1 , which further reduces risk of infection. (wikipedia.org)
  • Mucins are secreted as massive aggregates of proteins with molecular masses of roughly 1 to 10 million Da . (wikipedia.org)
  • Mucins (/ˈmjuːsɪn/) are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by epithelial tissues in most animals. (wikipedia.org)
  • Mucins are proteins that cover and protect epithelial cells and are characterized by domains rich in proline, threonine, and serine that are heavily glycosylated (PTS or mucin domains). (pnas.org)
  • A number of animal genomes were examined for these domains to identify mucin homologues, and domains of the resulting proteins were used in phylogenetic studies. (pnas.org)
  • This animal also has a unique family of secreted mucin-like proteins with alternating PTS and SEA domains, a type of protein also identified in the fishes. (pnas.org)
  • The evolution of the Muc4 mucin seems to have occurred by recruitment of a PTS domain to AMOP, NIDO, and VWD domains from a sushi domain-containing family of proteins present in lower animals, and Xenopus is the most deeply branching animal where a protein similar to the mammalian Muc4 was identified. (pnas.org)
  • In contrast, proteins with properties of the gel-forming mucins were identified also in the starlet sea anemone Nematostella vectensis , demonstrating an early origin of this group of mucins. (pnas.org)
  • With this definition of mucins, 14 proteins have been assigned to the MUC gene family according to the HUGO nomenclature ( www.genenames.org ), although this family contains proteins that differ considerably. (pnas.org)
  • Other proteins with shorter mucin domains, like IgA and several receptors, are not called mucins. (pnas.org)
  • All of the transmembrane mucins are type 1 proteins with one membrane spanning domain but are also characterized by other protein domains. (pnas.org)
  • Smaller proteins could enter the pores while mucins could not. (eurekalert.org)
  • Mucins are glycosylated proteins capable of forming gels. (biology-online.org)
  • Mucins are heavily glycosylated proteins. (biology-online.org)
  • Mucin coatings affect the conformation and neutrophil-activating properties of adsorbed host proteins-Toward amucosal mimic, Journal of Biomedical MaterialsResearch 91A, 2009, 773 - 785. (actapress.com)
  • The mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). (scbt.com)
  • Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. (scbt.com)
  • Mucins are glycoconjugated proteins which are secreted by vesicles and discharged on the surface of the conjunctival epithelium of the eye. (google.com)
  • In the case of contact lenses, mucins are often viewed as a debris that, like other proteins, should not accumulate on the contact lens surface. (google.com)
  • Mucins are a family of high molecular weight proteins which are densely glycosylated. (prolibraries.com)
  • Expression of mucins (MUC1, MUC2, MUC5AC, and MUC6) and type 1 Lewis antigens in cases with and without Helicobacter pylori colonization in metaplastic glands of the human stomach. (springer.com)
  • Two membrane mucins, MUC1 and MUC4 have been extensively studied in relation to their pathological implication in the disease process. (wikipedia.org)
  • All transmembrane mucins seem to have appeared in the vertebrate lineage, and the MUC1 mucin is restricted to mammals. (pnas.org)
  • However, both their cytoplasmic tails as well as the structure of the SEA domain suggest that they are involved in various signaling events as best studied for the MUC1 mucin ( 2 , 3 ). (pnas.org)
  • The major mucins produced in the airways are the secreted polymeric mucins MUC5AC and MUC5B [ 2 ] and the cell-tethered mucins MUC1, MUC4, MUC16 and MUC20 [ 3 ]. (biochemsoctrans.org)
  • In colorectal cancer (CRC), pathological factors that correlate with negative prognosis include, among others, overexpression of cyclooxygenase‑2 (COX‑2) and abundant expression of mucin 1 (MUC1). (spandidos-publications.com)
  • Mucin 1 (MUC1) is a structural membrane-bound mucin that is normally present only on the apical borders of the secretory epithelium. (spandidos-publications.com)
  • The mucin 1 (MUC1) heterodimeric protein is aberrantly overexpressed in human breast cancers and induces transformation. (aacrjournals.org)
  • Importantly, overexpression of MUC1-C, and not the MUC1-N mucin component, is sufficient to induce transformation and resistance to stress-induced apoptosis ( 11 , 13 , 16 - 18 ). (aacrjournals.org)
  • Analysis of gene and protein expression of Muc5ac and Muc5b in incipient CC lines ( n = 154) suggested that post-transcriptional events were important regulators of mucin protein content in the airways. (nih.gov)
  • The major secreted airway mucins are MUC5AC and MUC5B , while MUC2 is secreted mostly in the intestine but also in the airway. (wikipedia.org)
  • MUC5B, the predominate mucin in the mouth and female genital tract, has been shown to significantly reduce attachment and biofilm formation of Streptococcus mutans , a bacteria with the potential to form cavities. (wikipedia.org)
  • [8] Similar effects of MUC5B and other mucins have been demonstrated with other pathogens, such as Candida albicans , Helicobacter pylori , and even HIV . (wikipedia.org)
  • Tissue sections were stained with Alcian blue (AB)/periodic acid-Schiff (PAS), for mucins MUC5B and MUC5AC, and for neutrophils and its chemoattractant interleukin (IL) 8. (bmj.com)
  • Given the importance of MUC5AC and MUC5B in muco-obstructive airway diseases, we sought to identify key regulators of these secreted mucins using an unbiased, genome-wide search. (genetics.org)
  • Methods Sections were stained with H&E, Alcian blue-periodic acid Schiff (AB-PAS), polyclonal MUC5B antibody, and specific MUC5B riboprobe for histological, histochemical, immunohistochemical, and mucin mRNA analyses. (wiley.com)
  • In situ hybridization and immunohistochemistry studies demonstrated that the secretory cells of the middle ear mucosa with COM expressed MUC5B mucin mRNA and its product MUC5B mucin. (wiley.com)
  • Conclusions The MUC5B mucin gene and its product were identified in the middle ear secretory cells of patients with COM. (wiley.com)
  • The expression of a subset of mucins (MUC2, MUC6, MUC5B) was also correlated with sialyl-Tn expression in LS174T cells. (antibodies-online.com)
  • 2. Considerable heterogeneity was found with both antibody and lectin typing, but there was no significant difference in heterogeneity between mucin from patients with gallstones and control subjects. (clinsci.org)
  • All mucin preparations reacting with the polyclonal antibody also bound to Helix pomatia agglutinin. (clinsci.org)
  • There are currently no images for Mucin 5AC Antibody (NBP2-34758V). (novusbio.com)
  • Mucin 3 Antibody (M3.1) is a high quality monoclonal Mucin 3 antibody (also designated mucin 3A, cell surface associated antibody or MUC3A antibody) suitable for the detection of the Mucin 3 protein of human origin. (scbt.com)
  • Mucin 3 Antibody (M3.1) is available as the non-conjugated anti-Mucin 3 antibody. (scbt.com)
  • I am using Mucin 3 (M3.1): sc-7315 monoclonal antibody to stain paraffin embedded tissue. (scbt.com)
  • Immunohistochemistry-Paraffin: Mucin 5AC Antibody (45M1) - Azide and BSA Free [NBP2-32732] - Formalin-fixed, paraffin-embedded Rat Stomach stained with MUC5AC Monoclonal Antibody (45M1). (novusbio.com)
  • Immunohistochemistry-Paraffin: Mucin 5AC Antibody (45M1) [Azide Free] [NBP2-32732] - Human stomach. (novusbio.com)
  • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and 1 BP-HRP">m-IgG 1 BP-HRP (mouse IgG 1 binding protein-HRP) are the preferred secondary detection reagents for Mucin 5B Antibody (5B#19-2E) for WB applications. (scbt.com)
  • These reagents are now offered in bundles with Mucin 5B Antibody (5B#19-2E) ( see ordering information below ). (scbt.com)
  • Mucin 5B Antibody (5B#19-2E) is available as both the non-conjugated anti-Mucin 5B antibody form, as well as multiple conjugated forms of anti-Mucin 5B antibody, including agarose, PE, FITC and multiple Alexa Fluor ® conjugates. (scbt.com)
  • Abstract--"Mucin Based Immunity and Immunotherapy of Cancer", by O.J. Finn, Dept. of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA. (freepatentsonline.com)
  • [6] A recently identified human mucin gene is MUC21 , previously known as chromosome 6 open reading frame 205. (wikipedia.org)
  • Mucin-20 (MUC20) was identifiied as a gene that is up-regulated in the renal tissues of patients with immunoglobulin A nephropathy [ PMID: 14565953 ]. (ebi.ac.uk)
  • Mucin 4 (MUC 4) is a mucin protein that in humans is encoded by the MUC4 gene. (wikipedia.org)
  • In this study, we elucidated the effect of mucin on the gene expression and probiotic traits of A. muciniphila . (frontiersin.org)
  • Objectives To identify the mucin gene and its expressing cells in the middle ear mucosa with chronic otitis media (COM), and to study the correlation between infiltration of inflammatory cells in the submucosa and expression of the mucin gene in the mucosal epithelium with COM. (wiley.com)
  • The Mucin 5B gene, which contains a 3′ cis-element, is one of the four mucin genes mapped to human chromosome 11p15. (scbt.com)
  • Eosinophils were isolated from the peripheral blood of allergic patients, and their effects on MUC5AC mucin gene and protein synthesis were assessed using in situ hybridization and ELISAs. (jimmunol.org)
  • EGF, TGF-α) resulted in MUC5AC mucin gene and protein synthesis in human airway epithelial (NCI-H292) cells in vitro, and selective inhibitors of EGFR tyrosine kinase phosphorylation prevented ligand-induced mucin synthesis in vitro and prevented allergen-induced goblet cell metaplasia in pathogen-free rats ( 13 ). (jimmunol.org)
  • Inflammation of the large intestine and the inflammatory bowel disease ulcerative colitis is observed in mice that are deficient in the mucin MUC2 ( 5 ), suggesting that the mucins are important for intestinal handling of bacteria. (pnas.org)
  • Intestinal mucins from cystic fibrosis mice show increased fucosylation due to an induced Fucalpha1-2 glycosyltransferase. (gu.se)
  • This review will focus on what is known about goblet cell response in various GI infections and the regulatory networks that mediate goblet cell function and mucin production in response to intestinal insults. (mdpi.com)
  • In addition, we describe the current knowledge on the role of mucins in intestinal innate defense. (mdpi.com)
  • Although MUC13, a newly identified transmembrane mucin, is known to be aberrantly expressed in ovarian and gastro-intestinal cancers, its role in pancreatic cancer is unknown. (aacrjournals.org)
  • These transcript level changes were consistent with the fact that administration of A. muciniphila grown under mucin-depleted conditions to high-fat diet-induced diabetic mice reduced obesity and improved intestinal barrier integrity more efficiently than administration of A. muciniphila grown under mucin-containing conditions. (frontiersin.org)
  • In conclusion, mucin content in the growth medium plays a critical role in the improvement by A. muciniphila of high-fat diet-induced obesity, intestinal inflammation, and compromised intestinal barrier integrity related to a decrease in goblet cell density. (frontiersin.org)
  • The invertebrate intestinal mucin is a major protein constituent of the peritrophic membrane. (bioone.org)
  • X.N. Li , R.J. Li , D.Q. Xu , W. Guo , W.M. Sun , D. Zhao , and D.Q. Liu "Eukaryotic Expression and Biochemical Characterization of Intestinal Mucin SeIIM8 from Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae)," African Entomology 20(2), 252-258, (1 September 2012). (bioone.org)
  • The mucous secretions produced by some epithelial cells, particularly those lining the gastro-intestinal tract and the lungs, contain mucins along with other products and these components have been studied for some time at the biochemical level. (springer.com)
  • Intestinal mucins in colonization and host defense against pathogens. (ajtmh.org)
  • Intestinal mucins are key components of the first line of host defense against intestinal pathogens. (ajtmh.org)
  • Selective induction of mucin-3 by hypoxia in intestinal epithelia. (wikigenes.org)
  • In conclusion, increasing intestinal content viscosity with CMC in weaned piglets increased the ileal mucin output and numbers and maturation of goblet cells in ileal villi without effects on diet apparent digestibility. (europa.eu)
  • We found that porcine mucin glycans (PMGs) and HMOs enrich for taxonomically similar resident microbes. (nature.com)
  • Mucin glycans are built upon an N- acetyl-galactosamine that is O -linked to serine and threonine residues of the mucin protein, while HMOs are built upon a lactose core structure universally present at the reducing end of these glycans [ 6 ]. (nature.com)
  • In both mucin glycans and HMOs, the priming carbohydrate structure is extended with galactose- N- acetylglucosamine disaccharides and chains often terminate with fucose or sialic acid residues [ 7 ]. (nature.com)
  • The O-glycan structures present in mucin are diverse and complex, consisting predominantly of core 1-4 mucin-type O-glycans containing α- and β- linked N-acetyl-galactosamine, galactose and N-acetyl-glucosamine. (nih.gov)
  • This is largely due to the limited number of functionally characterized enzymes and the lack of studies correlating the specificity of these enzymes with the ability of the strain to degrade and utilize mucin and mucin glycans. (nih.gov)
  • This review focuses on recent findings unraveling the molecular strategies used by mucin-degrading bacteria to utilize host glycans, adapt to the mucosal environment, and influence human health. (nih.gov)
  • Significance: By attenuating the virulence of pathogens rather than eradicating them, mucin glycans are attractive candidates for novel therapeutics. (mrsec.org)
  • Mucin O-glycans can be branched, and many sugars or groups of sugars are antigenic. (genome.jp)
  • Important modifications of mucin O-glycans include O-acetylation of sialic acid and O-sulfation of galactose and N-acetylglucosamine. (genome.jp)
  • Their central protein backbone contains hydrophobic and charged domains, while the mucin-associated glycans provide hydrogen bonding capabilities, high hydration, and negative charges. (prolibraries.com)
  • Analysis of the O-glycans shows that the C-PSLex mucin has the longer and more branched side chains, but C-P55 has slightly higher sialic acid content. (diva-portal.org)
  • The [ 35 S]sulphate-labelled material yielded large trypsin-resistant fragments and contained O-linked glycans but was not affected by digestion with chondroitin ABC lyase or heparan sulphate lyase, suggesting that it is a mucin rather than a proteoglycan. (lu.se)
  • Colonic mucin synthesis is increased by sodium butyrate. (bmj.com)
  • Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. (scbt.com)
  • The ileal crude mucin concentration was determined by ethanol precipitation and changes in goblet cell subtypes were analyzed by histochemistry of ileal and colonic tissues. (europa.eu)
  • S. Gendler, J. Taylor-Papadimitriou, T. Duhig, J. Rothbard, and J. Burchell, A highly immunogenic region of a human polymorphic epithelial mucin expressed by carcinomas is made up of tandem repeats, J. Biol. (springer.com)
  • The boronic acid moieties may be complexed with mucin, especially epithelial mucin. (google.com)
  • To investigate the clinical significance of an immune response to the MUC-1 encoded polymorphic epithelial mucin (PEM) breast cancer, circulating immune complexes containing PEM (PEM.CIC) were measured in sera from 96 healthy women, in pretreatment serum samples from 40 patients with benign breast tumours and from 140 patients with breast cancer and in serum samples from 61 breast cancer patients with recurrent or progressive disease. (nih.gov)
  • Monoclonal antibodies specific for human polymorphic epithelial mucin. (freepatentsonline.com)
  • Bovine submaxillary mucin coatings Verotoxin-producing Escherichia coli Marin F, Luquet G, Marie B, Medakovic D (2007). (wikipedia.org)
  • The cysteine-knot is responsible for dimerization in the endoplasmic reticulum, and, in the case of porcine submaxillary mucin (PSM) and MUC2, the third von Willebrand D (VWD) domain in its N-terminal part is responsible for trimerization in the late secretory pathway ( 1 , 4 ). (pnas.org)
  • IL8 staining was increased in the epithelium of patients with cystic fibrosis and colocalised with mucins. (bmj.com)
  • Mucins anchor this tear film to the epithelium and protect the eye surface from bacterial, chemical and physical invasion of foreign bodies. (google.com)
  • In this report, we provide photographic evidence of this debris (sometimes termed "mucin balls" or "lipid plugs"), discuss its differential diagnosis and describe a case in which material seems to be embedded in the epithelium as a direct consequence of their presence. (biomedsearch.com)
  • These results show that activated eosinophils induce mucin synthesis in human airway epithelial cells via EGFR activation, and they implicate TGF-α produced by eosinophils and epithelial cells in the EGFR activation that results in mucin production in human airway epithelium. (jimmunol.org)
  • More recently mucin directed monoclonal antibodies have been used to target tumors with cytotoxic agents. (nih.gov)
  • J. Burchell, S. Gendler, J. Taylor-Papadimitriou, A. Girling, A. Lewis, R. Millis and D. Lamport, Development and characterization of breast cancer reactive monoclonal antibodies directed to the core protein of the human milk mucin, Cancer Res. (springer.com)
  • For this purpose polyclonal and monoclonal antibodies against gallbladder mucin were raised. (clinsci.org)
  • With these antibodies the immunoreactivity of gallbladder mucin from 60 patients with cholesterol gallstones and 20 subjects without stones was screened. (clinsci.org)
  • Mucin-2 (MUC2) belongs to the mucin family. (ebi.ac.uk)
  • This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. (biotium.com)
  • Mucins may be defined as having one long mucin domain rich in proline, threonine, and serine (PTS domain), which is heavily glycosylated through GalNAc O -linkage to the serine and threonine residues. (pnas.org)
  • MUC-4 mucins consist of a large extracellular alpha subunit that is heavily glycosylated and a beta subunit that is anchored in the cell membrane and extends into the cytosol. (wikipedia.org)
  • N-linked oligosaccharides are also found on mucins, but in less abundance than O-linked sugars. (wikipedia.org)
  • The ability to metabolize these mucin O-linked oligosaccharides is likely to be a key factor in determining which bacterial species colonize the mucosal surface. (nih.gov)
  • Hence, the implementation of urea makes the technique applicable for high throughput isolation and screening of mucin oligosaccharides by LC-MS after releasing the oligosaccharides from isolated, blotted mucin subpopulations. (gu.se)
  • Metabolomic software was also used to show that the migration of mucin isoforms within the gel is due to heterogeneous size distribution of the oligosaccharides, with the slower migrating bands enriched in high-molecular-weight oligosaccharides. (gu.se)
  • Humoral Immunity against a Tandem Repeat Epitope of Human Mucin MUC-1 in Sera from Breast, Pancreatic, and Colon Cancer Patients.sup.1, Advances in Brief, Cancer Research 54, 2856-2860, Jun. (freepatentsonline.com)
  • To identify genes that underlie variation in airway mucin levels, we performed genetic analyses in founder strains and incipient lines of the Collaborative Cross (CC) in a house dust mite mouse model of asthma. (nih.gov)
  • MUC genes: mucin or not mucin? (ebi.ac.uk)
  • Transcriptome analysis showed that the genes encoding most mucin-degrading enzymes were significantly upregulated in the presence of mucin. (frontiersin.org)
  • By contrast, most genes involved in glycolysis and energy metabolic pathways were upregulated under mucin-depleted conditions. (frontiersin.org)
  • A total of 21 mucin genes have been identified ( https://www.genenames.org ), which are divided into two families: the secreted mucins and the cell-tethered mucins. (biochemsoctrans.org)
  • Molecular cloning, genomic structure, and expression analysis of MUC20, a novel mucin protein, up-regulated in injured kidney. (ebi.ac.uk)
  • In this review, we will focus on the altered expression pattern of mucins in pancreatic cancer, from the early neoplastic lesion Pancreatic Intraepithelial Neoplasia (PanIN) to invasive pancreatic carcinomas, and the molecular mechanisms (including genetic and epigenetic regulation) and signaling pathways known to control their expression. (mdpi.com)
  • In infection, precancerous lesions and cancer, the expression and spatial distribution of mucins change. (gu.se)
  • Analysis of mucins was determined by ELISA and expression of TLR2 and TLR4 by flow cytometry. (dovepress.com)
  • Significant differences were observed neither in the expression of TLRs 2 and 4 in inflammatory cells of IS or peripheral blood, nor in the expression of mucins between both groups. (dovepress.com)
  • Increased expression of EGFR and its ligand suggests roles in mucin synthesis and neutrophil recruitment. (bmj.com)
  • however, for many types of cancer, mucin expression becomes altered (down-regulated, up-regulated or newly expressed). (atlasgeneticsoncology.org)
  • Therefore, the over-expression of mucins may be implicated in the exfoliation, dissemination and invasion of the cancer cells (Hollingsworth and Swanson, 2004). (atlasgeneticsoncology.org)
  • Certain microorganisms and disease states may alter mucin biochemistry or expression. (ajtmh.org)
  • Nineteen of the 21 reacting mucins (90%) were from patients with blood group A (18 patients) or AB (one patient) and expression of A antigen could be demonstrated on the mucin of these patients. (clinsci.org)
  • The resulting two reacting mucins were from patients with type O. However, expression of the blood group antigen could not account for the lack of reactivity of the mucin of other patients. (clinsci.org)
  • Label-free in vivo molecular imaging of underglycosylated mucin-1 expression in tumour cells. (sigmaaldrich.com)
  • Mucous differentiation and expression of Mucin 5B is retinoic acid- (RA) or retinol-dependent. (scbt.com)
  • To examine the relationship of growth pattern of colorectal carcinoma with mucin property and cellular proliferation of transitional mucosa (TM), 49 lesions of colorectal carcinomas invading to the muscularis propria were studied. (go.jp)
  • However cellular proliferation in TM did not differ by the growth pattern of carcinoma or the mucin property of TM. (go.jp)
  • Since carcinoma mucins can have binding sites for selectins, we hypothesized that selectin-mucin interactions might trigger this syndrome. (jci.org)
  • When highly purified, tissue-factor free carcinoma mucin preparations were intravenously injected into mice, platelet-rich microthrombi were rapidly generated. (jci.org)
  • Thus, Trousseau syndrome is likely triggered by interactions of circulating carcinoma mucins with leukocyte L-selectin and platelet P-selectin without requiring accompanying thrombin generation. (jci.org)
  • A case of 77-year-old female with an invasive lobular carcinoma with extracellular mucin is presented. (intechopen.com)
  • These findings suggested invasive carcinoma with mucin. (intechopen.com)
  • Interestingly, pathological diagnosis was invasive lobular carcinoma with extracellular mucin. (intechopen.com)
  • Mucins are also overexpressed in lung diseases such as asthma , bronchitis , chronic obstructive pulmonary disease (COPD) or cystic fibrosis . (wikipedia.org)
  • Overproduction of mucins in the lungs is a key element of both chronic obstructive lung disease and cystic fibrosis. (pnas.org)
  • Mucin is also overly expressed in the presence of lung diseases such as asthma , bronchitis , cystic fibrosis, etc . (biology-online.org)
  • The purpose of this study is to obtain structural information regarding potential pathways for airway plugging, mucin production and neutrophil recruitment that we hope will help in designing future mechanistic studies in patients with cystic fibrosis. (bmj.com)
  • We show that stimulation of EGF-R by its ligands results in mucin-producing goblet cells and that ovalbumin sensitization in rats causes induction of EGF-R and goblet-cell production in airways. (pnas.org)
  • One produces tremendous amounts of mucins which block the small airways, while the other two drive fibrosis and inflammation which together degrade the function of the lung," said Wa Xian, research associate professor of the Stem Cell Center. (rtmagazine.com)
  • Because an epidermal growth factor receptor (EGFR) cascade induces MUC5AC mucin in airways, and because EGFR is up-regulated in asthmatic airways, we examined the effect of eosinophils on MUC5AC mucin production in NCI-H292 cells (a human airway epithelial cell line that produces mucins). (jimmunol.org)
  • Mucin production in airways is induced by an epidermal growth factor receptor (EGFR) 3 cascade ( 13 ). (jimmunol.org)
  • Identification of trans Protein QTL for Secreted Airway Mucins in Mice and a Causal Role for Bpifb1. (nih.gov)
  • Mucins contribute to airway plugging. (bmj.com)
  • Typically, these mucins are found apically in polarized epithelia, but are distributed over the whole cell in cancer. (pnas.org)
  • The gel-forming mucins are instrumental in the protection of the underlying epithelia and in trapping and elimination of bacteria. (pnas.org)
  • Mucins are found on moist, mucosal epithelia, and are thought to combine mechanical protection of eye tissue as well as chemical and immune protection of mucosal tissue. (google.com)
  • This cell type often showed supranuclear lysozyme reactivity and apical neutral mucins, sialomucins, and sulphomucins in variable amounts. (bmj.com)
  • Trousseau syndrome often occurs with mucinous adenocarcinomas, which secrete abnormally glycosylated mucins and mucin fragments into the bloodstream. (jci.org)
  • There are two main classes of mucins: membrane-bound mucins, contributing to the composition of the cellular glycocalyx, and secreted mucins, contributing to the composition of the extracellular matrix or the mucous barrier that covers all epithelial surfaces in the human body, except the skin. (springer.com)
  • The mechanisms for the functioning of the mucin-based defense system are likely to be similar for many of the disease-producing organisms that infect mucous membranes. (gu.se)
  • Salivary mucins are also crucial to the balance of calcium in the mucous membranes. (reference.com)
  • The primary functions of mucins are to hydrate, protect, and lubricate the epithelial surfaces within the human body. (springer.com)
  • The human gel-forming mucins contain VWD and C-terminal cysteine-knot domains that are responsible for the oligomerization of these molecules. (pnas.org)
  • Synthetic peptide corresponding to Human Mucin 5AC aa 5000 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. (abcam.com)
  • ab77576 is specific for the MUC5AC tandem repeat of human mucin. (abcam.com)
  • Our findings suggest the depletion of animal-derived mucin in growth medium as a novel principle for the development of A. muciniphila for human therapeutics. (frontiersin.org)
  • 1. Human gallbladder mucin has been implicated to play a role in gallstone disease. (clinsci.org)
  • In spite of this fact relatively little is known about the structure of human gallbladder mucin. (clinsci.org)
  • 3. We conclude that considerable patient to patient heterogeneity of human gallbladder mucin exists. (clinsci.org)
  • mucin from human ovarian cyst fluid. (sigmaaldrich.com)
  • Human TTF2 is a lectin that binds alpha-GlcNAc-capped mucin 6 g with antibiotic activity against Helicobacter pylori. (antibodies-online.com)
  • Although Mucin 5B is the prominent human gallbladder mucin, it is also expressed and secreted in the colon. (scbt.com)
  • Biophysical Characterization of One-, Two-, and Three-Tandem Repeats of Human Mucin (muc-1) Protein Core.sup.1, Cancer Research 53, 5386-5394, Nov. 15, 1993. (freepatentsonline.com)
  • Mucin also lubricates the tissues and teeth in the mouth and protects them against pathogens and toxins. (reference.com)
  • Once these stores are depleted, mucin production reverts to baseline levels, which presumably is not capable of supporting large numbers of pathogens. (beefresearch.org)
  • Members of the mucin family are characterized by a hallmark feature: the presence of a tandem repeat domain, consisting of a protein backbone which acts as a scaffold for a large number of complex O-linked carbohydrate side chains (Williams et al. (atlasgeneticsoncology.org)
  • The interaction between microbes and mucins involves mucin carbohydrate side chains and microbial adhesin molecules. (ajtmh.org)
  • Mucins share a common structural feature which is a tandem repeat domain comprising a variable number of tandem repeats (VNTRs) of identical or highly similar sequences rich. (springer.com)
  • The mucin family has been under study by molecular biologists, biochemists, pathologists and immunologists interested in cancer because of the role these molecules can play in the diagnosis and treatment of cancer. (nih.gov)
  • We found that by adding glycerol to the aqueous solvent, we could induce drastic condensation of purified mucin molecules, reducing their size by an order of magnitude down to tens of nanometers in diameter. (diva-portal.org)
  • The change of structure of mucins from extended molecules to nano-sized particles in the presence of glycerol translated into macroscopic rheological changes, as illustrated by a dampened shear-thinning effect with increasing glycerol concentration. (diva-portal.org)
  • For cells grown on collagen, the amount of the sialic-acid-rich mucin increased over 10 days, whereas the PAS-reactive component was largely absent after 24 h, which was consistent with an initial release of stored PAS-reactive molecules and synthesis of the sialic-acid-rich mucins de novo. (lu.se)
  • 2001). In general, mucins have important biological roles in the lubrication and protection of normal epithelial tissues. (atlasgeneticsoncology.org)
  • The major functions of mucins include cell signaling, lubrication, and forming chemical barriers. (biology-online.org)
  • The increase in response to butyrate was not seen when specimens were incubated in the presence of the beta oxidation inhibitor sodium bromo-octanoate 0.05 M. The striking increase in mucin synthesis that results when butyrate is added to standard nutrient medium suggests that this may be an important mechanism affecting the rate of mucin synthesis in vivo and may also explain the therapeutic effect of butyrate in colitis. (bmj.com)
  • When overexpressed, the disorganization of mucins may reduce adhesion to other cells as well as the extracellular matrix, promoting cancer cell migration and metastasis. (wikipedia.org)
  • The cell surface mucins provide a barrier and signaling function and are involved in tumour metastasis as well as regulation of cell proliferation. (gu.se)
  • Preoperative assessment of the growth pattern of lesions and the mucin property of TM appears to be useful for predicting lymphnode metastasis of colorectal carcinomas invading to the muscularis propria. (go.jp)
  • It is the aim of this review to provide the readers with an update on goblet cell biology and current understanding on the role of mucins in host defense in enteric infections. (mdpi.com)
  • The mucin domains often contain tandemly repeated sequences and vary to a great extent in sequence and length depending on allele, species, and individual mucin ( 1 , 2 ). (pnas.org)
  • n = 5 individual mucin preparations. (jci.org)
  • Frequently in tumors of the pancreas there are changes in the structure of mucin carbohydrates and/or levels of apomucin types. (nih.gov)
  • The carbohydrates of mucins on the surface of tumor cells have been reported to inhibit cells of the immune system. (nih.gov)
  • Mucin is a glycosated protein, which means that it is coated with carbohydrates. (reference.com)
  • Abnormal deposits of mucin are responsible for the non-pitting facial edema seen in untreated hypothyroidism. (wikipedia.org)
  • Dermal deposits of mucin in the legs have been described associated with venous insufficiency. (ovid.com)