Simple sugars, carbohydrates which cannot be decomposed by hydrolysis. They are colorless crystalline substances with a sweet taste and have the same general formula CnH2nOn. (From Dorland, 28th ed)
A ketose sugar that is commonly used in the commercial synthesis of ASCORBIC ACID.
A large group of membrane transport proteins that shuttle MONOSACCHARIDES across CELL MEMBRANES.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.
A broad category of membrane transport proteins that specifically transport FREE FATTY ACIDS across cellular membranes. They play an important role in LIPID METABOLISM in CELLS that utilize free fatty acids as an energy source.
Membrane proteins whose primary function is to facilitate the transport of molecules across a biological membrane. Included in this broad category are proteins involved in active transport (BIOLOGICAL TRANSPORT, ACTIVE), facilitated transport and ION CHANNELS.
The movement of materials across cell membranes and epithelial layers against an electrochemical gradient, requiring the expenditure of metabolic energy.
Transport proteins that carry specific substances in the blood or across cell membranes.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Membrane proteins whose primary function is to facilitate the transport of positively charged molecules (cations) across a biological membrane.
The movement of ions across energy-transducing cell membranes. Transport can be active, passive or facilitated. Ions may travel by themselves (uniport), or as a group of two or more ions in the same (symport) or opposite (antiport) directions.
Membrane proteins whose primary function is to facilitate the transport of negatively charged molecules (anions) across a biological membrane.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.
The rate dynamics in chemical or physical systems.
The directed transport of ORGANELLES and molecules along nerve cell AXONS. Transport can be anterograde (from the cell body) or retrograde (toward the cell body). (Alberts et al., Molecular Biology of the Cell, 3d ed, pG3)
Oligosaccharides containing two monosaccharide units linked by a glycosidic bond.
The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Membrane transporters that co-transport two or more dissimilar molecules in the same direction across a membrane. Usually the transport of one ion or molecule is against its electrochemical gradient and is "powered" by the movement of another ion or molecule with its electrochemical gradient.
The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.
Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
The characteristic 3-dimensional shape of a carbohydrate.
A hexose or fermentable monosaccharide and isomer of glucose from manna, the ash Fraxinus ornus and related plants. (From Grant & Hackh's Chemical Dictionary, 5th ed & Random House Unabridged Dictionary, 2d ed)
Cellular processes in biosynthesis (anabolism) and degradation (catabolism) of CARBOHYDRATES.
A family of monosaccharide transport proteins characterized by 12 membrane spanning helices. They facilitate passive diffusion of GLUCOSE across the CELL MEMBRANE.
Proteins found in any species of bacterium.
The N-acetyl derivative of glucosamine.
A member of the alkali group of metals. It has the atomic symbol Na, atomic number 11, and atomic weight 23.
A broad category of proteins involved in the formation, transport and dissolution of TRANSPORT VESICLES. They play a role in the intracellular transport of molecules contained within membrane vesicles. Vesicular transport proteins are distinguished from MEMBRANE TRANSPORT PROTEINS, which move molecules across membranes, by the mode in which the molecules are transported.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A monosaccharide in sweet fruits and honey that is soluble in water, alcohol, or ether. It is used as a preservative and an intravenous infusion in parenteral feeding.
A major integral transmembrane protein of the ERYTHROCYTE MEMBRANE. It is the anion exchanger responsible for electroneutral transporting in CHLORIDE IONS in exchange of BICARBONATE IONS allowing CO2 uptake and transport from tissues to lungs by the red blood cells. Genetic mutations that result in a loss of the protein function have been associated with type 4 HEREDITARY SPHEROCYTOSIS.
A family of MEMBRANE TRANSPORT PROTEINS that require ATP hydrolysis for the transport of substrates across membranes. The protein family derives its name from the ATP-binding domain found on the protein.
Any compound that contains a constituent sugar, in which the hydroxyl group attached to the first carbon is substituted by an alcoholic, phenolic, or other group. They are named specifically for the sugar contained, such as glucoside (glucose), pentoside (pentose), fructoside (fructose), etc. Upon hydrolysis, a sugar and nonsugar component (aglycone) are formed. (From Dorland, 28th ed; From Miall's Dictionary of Chemistry, 5th ed)
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Proteins that bind to and are involved in the metabolism of phosphate ions.
The process by which ELECTRONS are transported from a reduced substrate to molecular OXYGEN. (From Bennington, Saunders Dictionary and Encyclopedia of Laboratory Medicine and Technology, 1984, p270)
The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.
Membrane transporters that co-transport two or more dissimilar molecules in the opposite direction across a membrane. Usually the transport of one ion or molecule is against its electrochemical gradient and is "powered" by the movement of another ion or molecule with its electrochemical gradient.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Polyhydric alcohols having no more than one hydroxy group attached to each carbon atom. They are formed by the reduction of the carbonyl group of a sugar to a hydroxyl group.(From Dorland, 28th ed)
Vesicles that are involved in shuttling cargo from the interior of the cell to the cell surface, from the cell surface to the interior, across the cell or around the cell to various locations.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Proteins obtained from ESCHERICHIA COLI.
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Proteins involved in the transport of organic anions. They play an important role in the elimination of a variety of endogenous substances, xenobiotics and their metabolites from the body.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Cellular proteins and protein complexes that transport amino acids across biological membranes.
Uptake of substances through the lining of the INTESTINES.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
The N-acetyl derivative of galactosamine.
Periplasmic proteins that scavenge or sense diverse nutrients. In the bacterial environment they usually couple to transporters or chemotaxis receptors on the inner bacterial membrane.
Inorganic compounds derived from hydrochloric acid that contain the Cl- ion.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
A methylpentose whose L- isomer is found naturally in many plant glycosides and some gram-negative bacterial lipopolysaccharides.
A sequence-related subfamily of ATP-BINDING CASSETTE TRANSPORTERS that actively transport organic substrates. Although considered organic anion transporters, a subset of proteins in this family have also been shown to convey drug resistance to neutral organic drugs. Their cellular function may have clinical significance for CHEMOTHERAPY in that they transport a variety of ANTINEOPLASTIC AGENTS. Overexpression of proteins in this class by NEOPLASMS is considered a possible mechanism in the development of multidrug resistance (DRUG RESISTANCE, MULTIPLE). Although similar in function to P-GLYCOPROTEINS, the proteins in this class share little sequence homology to the p-glycoprotein family of proteins.
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. Some carbohydrate-metabolizing proteins (ENZYMES) from PLANTS also bind to carbohydrates, however they are not considered lectins. Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. They may play a role in plant defense mechanisms.
A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
A non-metabolizable glucose analogue that is not phosphorylated by hexokinase. 3-O-Methylglucose is used as a marker to assess glucose transport by evaluating its uptake within various cells and organ systems. (J Neurochem 1993;60(4):1498-504)
Proteins involved in the transport of NUCLEOTIDES across cellular membranes.
Established cell cultures that have the potential to propagate indefinitely.
Carbohydrates covalently linked to a nonsugar moiety (lipids or proteins). The major glycoconjugates are glycoproteins, glycopeptides, peptidoglycans, glycolipids, and lipopolysaccharides. (From Biochemical Nomenclature and Related Documents, 2d ed; From Principles of Biochemistry, 2d ed)
Proteins involved in the transport of NUCLEOSIDES across cellular membranes.

Mechanisms related to [18F]fluorodeoxyglucose uptake of human colon cancers transplanted in nude mice. (1/3673)

[18F]Fluorodeoxyglucose ([18F]FDG), a glucose analogue, has been widely used for tumor imaging. To investigate the mechanisms related to [18F]FDG uptake by tumors, an experiment involving nude mice was performed. METHODS: Human colon cancer cell lines SNU-C2A, SNU-C4 and SNU-C5 were transplanted to nude mice. Using immunohistochemical staining and Western blot, the expression of glucose transporter (Glut) isoforms (Glut-1 through -5) in xenografted tumors was analyzed. For the analysis of messenger ribonucleic acid (mRNA) expression, reverse-transcription polymerase chain reaction and Northern blot were used and the enzyme activity of hexokinase in cancer tissues was measured by continuous spectrophotometric rate determination. RESULTS: [18F]FDG uptake in SNU-C4 and SNU-C5 cells was higher than in normal colon cells. Among these cells and xenografted tumors, SNU-C5 showed the highest level of [18F]FDG uptake, followed by SNU-C4 and SNU-C2A. An immunostaining experiment showed intense staining of Glut-1 in SNU-C5 tumors but somewhat faint staining in SNU-C4. SNU-C5 tumors also showed positive staining with Glut-3, although this was not the case with SNU-C2A and SNU-C4. Western blot analysis showed the expression of Glut-1 and Glut-3 in all tumors. Experiments involving Northern blot analysis and reverse-transcription polymerase chain reaction confirmed the overexpression of Glut-1 mRNA in all tumors, with the highest level in SNU-C5. The level of Glut-3 mRNA was also elevated in SNU-C5 tumors but not in SNU-C2A and SNU-C4. The enzyme activity of hexokinase did not vary among different tumors. CONCLUSION: Gluts, especially Glut-1, are responsible for [18F]FDG uptake in a nude mouse model of colon cancer rather than hexokinase activity. Increased numbers of glucose transporters at the plasma membrane of cancer cells is attributed to an increased level of transcripts of glucose transporter genes and may be a cause of increased [18F]FDG uptake, at least in colon cancer tumors.  (+info)

A possible role for the pentose phosphate pathway of spermatozoa in gamete fusion in the mouse. (2/3673)

Glucose metabolism is essential for successful gamete fusion in the mouse. Although the metabolic activity of the oocyte does not appear to play a significant role in the fusion step, the metabolic role of the spermatozoon is not known. The aim of this study was therefore to characterize the role of glucose metabolism in mouse spermatozoa. Initially, the high-affinity glucose transporter GLUT3 was identified in mouse sperm. In characterizing the glucose metabolism of mouse sperm, we have shown 1) that mouse epididymal spermatozoa have a functional pentose phosphate pathway (PPP), implying that they produce NADPH, which is required for reducing reactions, and ribose 5-phosphate, which is required for nucleic acid synthesis; and 2) that sperm are able to fuse with the oocyte when NADPH is substituted for glucose, suggesting that sperm need to produce NADPH via the PPP in order to be able to achieve fertilization. The existence of an NADPH-regulated event that influences the ability of the sperm to fuse with the oocyte is envisaged.  (+info)

Inactivation of the glucose 6-phosphate transporter causes glycogen storage disease type 1b. (3/3673)

Glycogen storage disease type 1b (GSD-1b) is proposed to be caused by a deficiency in microsomal glucose 6-phosphate (G6P) transport, causing a loss of glucose-6-phosphatase activity and glucose homeostasis. However, for decades, this disorder has defied molecular characterization. In this study, we characterize the structural organization of the G6P transporter gene and identify mutations in the gene that segregate with the GSD-1b disorder. We report the functional characterization of the recombinant G6P transporter and demonstrate that mutations uncovered in GSD-1b patients disrupt G6P transport. Our results, for the first time, define a molecular basis for functional deficiency in GSD-1b and raise the possibility that the defective G6P transporter contributes to neutropenia and neutrophil/monocyte dysfunctions characteristic of GSD-1b patients.  (+info)

Missense mutations in SGLT1 cause glucose-galactose malabsorption by trafficking defects. (4/3673)

Glucose-galactose malabsorption (GGM) is an autosomal recessive disorder caused by defects in the Na+/glucose cotransporter (SGLT1). Neonates present with severe diarrhea while on any diet containing glucose and/or galactose [1]. This study focuses on a patient of Swiss and Dominican descent. All 15 exons of SGLT1 were screened using single stranded conformational polymorphism analyses, and aberrant PCR products were sequenced. Two missense mutations, Gly318Arg and Ala468Val, were identified. SGLT1 mutants were expressed in Xenopus laevis oocytes for radiotracer uptake, electrophysiological experiments, and Western blotting. Uptakes of [14C]alpha-methyl-d-glucoside by the mutants were 5% or less than that of wild-type. Two-electrode voltage-clamp experiments confirmed the transport defects, as no noticeable sugar-induced current could be elicited from either mutant [2]. Western blots of cell protein showed levels of each SGLT1 mutant protein comparable to that of wild-type, and that both were core-glycosylated. Presteady-state current measurements indicated an absence of SGLT1 in the plasma membrane. We suggest that the compound heterozygote missense mutations G318R and A468V lead to GGM in this patient by defective trafficking of mutant proteins from the endoplasmic reticulum to the plasma membrane.  (+info)

Altered substrate selectivity in a mutant of an intrahelical salt bridge in UhpT, the sugar phosphate carrier of Escherichia coli. (5/3673)

Site-directed and second site suppressor mutagenesis identify an intrahelical salt bridge in the eleventh transmembrane segment of UhpT, the sugar phosphate carrier of Escherichia coli. Glucose 6-phosphate (G6P) transport by UhpT is inactivated if cysteine replaces either Asp388 or Lys391 but not if both are replaced. This suggests that Asp388 and Lys391 are involved in an intrahelical salt bridge and that neither is required for normal UhpT function. This interpretation is strengthened by the finding that mutations at Lys391 (K391N, K391Q, and K391T) are recovered as revertants of the inactive D388C variant. Further work shows that although the D388C variant is null for G6P transport, movement of 32Pi by homologous Pi/Pi exchange is unaffected. This raises the possibility that this derivative may have latent function, a possibility confirmed by showing that D388C is a gain-of-function mutation in which phosphoenolpyruvate (PEP) is the preferred substrate. Added study of the Pi/Pi exchange shows that in wild type UhpT this partial reaction is readily blocked by G6P but not PEP. By contrast, in the D388C variant, Pi/Pi exchange is unaffected by G6P but is inhibited by both PEP and 3-phosphoglycerate. These latter substrates are used by PgtP, a related Pi-linked antiporter, which lacks the Asp388-Lys391 salt bridge but has instead an uncompensated arginine at position 391. For this reason, we conclude that in both UhpT and PgtP position 391 can serve as a determinant of substrate selectivity by acting as a receptor for the anionic carboxyl brought into the translocation pathway by PEP.  (+info)

MalK forms a dimer independent of its assembly into the MalFGK2 ATP-binding cassette transporter of Escherichia coli. (6/3673)

The maltose transport complex (MTC) is a member of the ATP-binding cassette superfamily of membrane transport proteins and is a model for understanding the folding and assembly of hetero-oligomeric membrane protein complexes. The MTC is made up of two integral membrane proteins, MalF and MalG, and a peripheral membrane protein, MalK. These proteins associate with a stoichiometry of 1:1:2 to form the complex MalFGK2. In our studies of the oligomerization of this complex, we have shown that the ATP-binding component, MalK, forms a dimer in the absence of MalF and MalG. Epitope-tagged MalK coimmunoprecipitated with wild-type MalK, indicating that the MalK protein forms an oligomer. The relative amounts of tagged and wild-type MalK that were present in the whole cell extracts and in the immunoprecipitated complexes show that the MalK oligomer is a dimer. These hetero-oligomers can also be formed in vitro by mixing two extracts, each containing either tagged or wild-type MalK. The dimerization of MalK was also demonstrated in vivo using the bacteriophage lambda repressor fusion assay. The formation of a MalK dimer in the absence of MalF and MalG may represent an initial step in the assembly pathway of the MTC.  (+info)

SNAP-23 participates in SNARE complex assembly in rat adipose cells. (7/3673)

SNARE proteins are required for vesicle docking and fusion in eukaryotic cells in processes as diverse as homotypic membrane fusion and synaptic vesicle exocytosis [SNARE stands for SNAP receptor, where SNAP is soluble NSF attachment protein]. The SNARE proteins syntaxin 4 and vesicle-associated membrane protein (VAMP) 2/3 also participate in the insulin-stimulated translocation of GLUT4 from intracellular vesicles to the plasma membrane in adipose cells. We now report the molecular cloning and characterization of rat SNAP-23, a ubiquitously expressed homologue of the essential neuronal SNARE protein SNAP-25 (synaptosomal-associated protein of 25 kDa). Rat SNAP-23 is 86% and 98% identical respectively to human and mouse SNAP-23. Southern blot analysis reveals that the rat, mouse and human SNAP-23 genes encode species-specific isoforms of the same protein. Co-immunoprecipitation of syntaxin 4 and SNAP-23 shows association of these two proteins in rat adipose cell plasma membranes, and insulin stimulation does not alter the SNAP-23/syntaxin 4 complex. In addition, we demonstrate for the first time the participation of SNAP-23, along with syntaxin 4 and VAMP2/3, in the formation of 20S SNARE complexes prepared using rat adipose cell membranes and recombinant alpha-SNAP and NSF proteins. The stoichiometry of the SNARE complexes formed is essentially identical using membranes from either unstimulated or insulin-stimulated adipose cells. These data demonstrate that rat SNAP-23 associates with syntaxin 4 before insulin stimulation and is present in the SNARE complexes known to mediate the translocation of GLUT4 from intracellular vesicles to the plasma membrane of rat adipose cells.  (+info)

Tumour necrosis factor-alpha regulates expression of the CCAAT-enhancer-binding proteins (C/EBPs) alpha and beta and determines the occupation of the C/EBP site in the promoter of the insulin-responsive glucose-transporter gene in 3T3-L1 adipocytes. (8/3673)

We have demonstrated previously that treatment of 3T3-L1 adipocytes with tumour necrosis factor-alpha (TNF) results in a rapid (4 h) and significant (75-80%) reduction in the rate of transcription of the GLUT4 gene. Control of GLUT4 gene transcription has been suggested at least in part to reside with the CCAAT-enhancer-binding protein (C/EBP) family (alpha, beta and delta isoforms) of transcription factors. Using electrophoretic mobility shift assays, we have examined the ability of TNF to alter the occupation of the C/EBP site in the GLUT4 promoter. The data suggest that in fully differentiated adipocytes the C/EBP site is a ligand for predominantly alpha/alpha homodimers; however, after exposure to TNF, a shift in occupancy of the site occurs and the ligands become alpha/beta heterodimers and beta/beta homodimers. Partner selection in dimer formation appears to be controlled by selective translocation of the beta-isoform from the cytosol to the nucleus after exposure of the cells to TNF.  (+info)

In this study, echocardiography was used to monitor the progression of LV hypertrophy and to determine the timing for the study of glucose uptake and glucose transporter protein content. The observations derived from this investigation reveal that a decrease in glucose uptake rate is evident early in the transition from compensated to decompensated pressure-overload hypertrophy, as determined by an index of LV M/V ratio. The defect in insulin-stimulated glucose uptake precedes the downregulation of sarcoplasmic reticulum Ca-ATPase (SERCA-2) and glucose transporter expression (GLUT-4 and GLUT-1).15 26 Under pathophysiological conditions such as hypertrophy or during ischemia and early reperfusion, a high rate of cardiac glucose metabolism may be crucial.27 28 29 Glucose transport is thought to be rate-limiting for glucose use. In the heart, 2 distinctive glucose transporters are responsible for glucose uptake across the plasma membrane. The GLUT-1 transporter, which is present in low levels in ...
The human glucose transporter Glut1 is used by many cells, especially red blood cells andbrain cell, to take up glucose and other necessary metabolites. Gentle chromatographic methods can be applied for analyses of Glut1 activities in lipid environments similar to the native one. For this purpose, methods for immobilization of cells, cytoskeleton-depleted red cell membrane vesicles and proteoliposomes in chromatographic gel media have been developed and refined. The vesicles and proteoliposomes were immobilized by freeze-thaw-induced fusion in dextran-grafted agarose gel beads (Superdex 200). The sheltered environment in microcavities kept the Glut1 active for repeated analyses over time periods up to three months at room temperature.. Frontal affinity chromatography, Hummel and Dreyer analyses and centrifugation methods revealed that the Glut1 affinities for inhibitors and D-glucose were highest in the cells andbecame successively lower upon cytoskeleton-depletion and subsequent solubilization ...
TY - JOUR. T1 - Direct effects of ionizing radiation on integral membrane proteins. T2 - Noncovalent energy transfer requires specific interpeptide interactions. AU - Jhun, E.. AU - Jhun, B. H.. AU - Jones, L. R.. AU - Jung, C. Y.. PY - 1991. Y1 - 1991. N2 - The 12 transmembrane α helices (TMHs) of human erythrocyte glucose transporter were individually cut by pepsin digestion as membrane-bound 2.5-3.5-kDa peptide fragments. Radiation-induced chemical degradation of these fragments showed an average target size of 34 kDa. This is 10-12 × larger than the average size of an individual TMH, demonstrating that a significant energy transfer occurs among these TMHs in the absence of covalent linkage. Heating this TMH preparation at 100 °C for 15 min reduced the target size to 5 kDa or less, suggesting that the noncovalent energy transfer requires specific helix-helix interactions. Purified phospholamban, a small (6-kDa) integral membrane protein containing a single TMH, formed a pentameric assembly ...
This gene belongs to the solute carrier 2A family, which includes intracellular glucose transporters. Based on sequence comparison, the glucose transporters are grouped into three classes and this gene is a member of class II. The encoded protein, like other members of the family, contains several conserved residues and motifs and 12 transmembrane domains with both amino and carboxyl ends being on the cytosolic side of the membrane. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Nov 2012 ...
I have heard that there is a distributor for antibodies directed against the Glut-1 glucose-transporter (erythroid/brain or perivenular hepatocytes). Does anyone know this distributor ? Thanks in advance ##################################################################### # # # Dr. F.Gaunitz __________ # # Physiologisch-Chemisches Institut I I # # Universitaet Tuebingen I \/ I # # Hoppe-Seyler-Str. 4 I U /\ T I # # 72076 Tuebingen I / \ I # # Germany \ + + / # # \______/ # # e-mail: cbkga01 at mailserv.zdv.uni-tuebingen.de ...
Diabetic Socks Knee Length Pre Con Sintomas Insulin-stimulated glucose uptake is primarily mediated by the transporter isoform GLUT4 Biochemistry and Cell Biology 2002 80(5): 569-578 10.1139/o02-156. # symptoms of type 1 diabetes in toddlers - paleo diet for. Diabetic Socks Knee Length Pre Con Sintomas diabetes dizzinessvertigo and mental confusion should never be ignored.. Healthy Banana Cream Tiramisu ArielleDuke. Diabetes type; 7) Diabetes type; 4) Code if any for coding diabetes mellitus: Type 2 Diabetes mellitus E10 Type 1 Diabetes mellitus E13 UNC Family Medicines weekly health radio show. Ever heard of gestational diabetes? Its when a when a woman develops diabetes during pregnancy.. The inslin diabetes update berlin mortality depression receptor consists of two units that come together (dimerize) when they bind with insulin to form an active tyrosine kinase enzyme (fig. The diabetes meal plan per day. Exercise-induced modulation of antioxidant Diabetic Socks Knee Length Pre Con ...
TY - JOUR. T1 - Heterogeneity in the rat adipocyte glucose transporter. AU - Baly, D. L.. AU - Simpson, Ian. AU - Matthaei, S.. AU - Horuk, R.. PY - 1985. Y1 - 1985. UR - http://www.scopus.com/inward/record.url?scp=0021919940&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0021919940&partnerID=8YFLogxK. M3 - Article. AN - SCOPUS:0021919940. VL - 44. JO - Federation Proceedings. JF - Federation Proceedings. SN - 0014-9446. IS - 3. ER - ...
GLUT4 antibody [1F8] (solute carrier family 2 (facilitated glucose transporter), member 4) for ICC/IF, IHC-Fr, IHC-P, IP, WB. Anti-GLUT4 mAb (GTX31226) is tested in Human, Mouse, Monkey, Pig, Rat, Rabbit samples. 100% Ab-Assurance.
Now lets try to describe each process announced. So phagocytosis is process of eating external organells by the cell. It is quite close to endocytosis, when - in contrast - molecules are being absorbed. Exocytosis is opposite process - secretion of molecules into external space. It can be hormones, antibodies and so on. Transcytosis is interesting process of transporting molecules from apical (absorbing) space of polarized cells to basolateral space, which happens, for example, when antibodies are transported through baby rats gut. Main idea is that molecules are absorbed into internal vesicles (as during endocytosis) and then after several steps containers content is exposed as during exocytosis. No target molecules are left in cells plasma. Interesting is that cell can regulate exposure of some proteins in the cell membrane using transcytosis. During latent phase (e.g. while there is no great need in glucose) transporters are stored incorporated in vesicles inside the cell. But in case of ...
Rabbit polyclonal GLUT12 antibody. Validated in IHC, Flow Cyt and tested in Mouse, Rat, Human. Immunogen corresponding to synthetic peptide.
TY - JOUR. T1 - Intracellular insulin-responsive glucose transporter (GLUT4) distribution but not insulin-stimulated GLUT4 exocytosis and recycling are microtubule dependent. AU - Shigematsu, Satoshi. AU - Khan, Ahmir H.. AU - Kanzaki, Makoto. AU - Pessin, Jeffrey E.. PY - 2002. Y1 - 2002. N2 - To investigate the potential role of microtubules in the regulation of insulin-responsive glucose transporter (GLUT4) trafficking in adipocytes, we examined the effects of microtubule depolymerizing and stabilizing agents. In contrast to previous reports, disruption or stabilization of microtubule structures had no significant effect on insulin-stimulated GLUT4 translocation. However, consistent with a more recent study (Molero, J. C., J. P. Whitehead, T. Meerloo, and D. E. James, 2001, J Biol Chem 276:43829-43835) nocodazole did inhibit glucose uptake through a direct interaction with the transporter itself independent of the translocation process. In addition, the initial rate of GLUT4 endocytosis was ...
The effect of insulinopenic diabetes on the expression of glucose transporters in the small intestine was investigated. Enterocytes were sequentially isolated from jejunum and ileum of normal fed rats, streptozotocin-diabetic rats, and diabetic rats treated with insulin. Facilitative glucose transporter (GLUT) 2, GLUT5, and sodium-dependent glucose transporter 1 protein content was increased from 1.5- to 6-fold in enterocytes isolated from diabetic animals in both jejunum and ileum. Insulin was able to reverse the increase in transporter protein expression seen after induction of diabetes. There was a four- to eightfold increase in the amount of enterocyte glucose transporter mRNA after diabetes with greater changes in sodium-dependent glucose transporter 1 and GLUT2 than in GLUT5 levels. In situ hybridization showed that after the induction of diabetes there was new hybridization in lower villus and crypt enterocytes that was reversed by insulin treatment. Thus, the increase in total hexose ...
Buy our Human Glucose Transporter GLUT4 peptide. Ab34088 is a blocking peptide for ab33780 and has been validated in BL. Abcam provides free protocols, tips…
It has been postulated that a glucose transporter of beta cells (GLUT-2) may be important in glucose-stimulated insulin secretion. To determine whether this transporter is constitutively expressed or regulated, we subjected conscious unrestrained Wistar rats to perturbations in glucose homeostasis and quantitated beta-cell GLUT-2 mRNA by in situ hybridization. After 3 hr of hypoglycemia (glucose at 29 +/- 5 mg/dl), GLUT-2 and proinsulin mRNA signal densities were reduced by 25% of the level in control rats. After 4 days (blood glucose at 57 +/- 7 mg/dl vs. 120 +/- 10 mg/dl in saline-infused control rats), GLUT-2 and proinsulin mRNA densities were reduced by 85% and 65%, respectively (P = 0.001). After 12 days (glucose at 54 +/- 8 mg/dl), GLUT-2 mRNA signal density was undetectable whereas proinsulin mRNA was reduced by 51%. After 12 days of hypoglycemia, the Km for 3-O-methyl-D-glucose transport in isolated rat islets, normally 18-20 mM, was 2.5 mM. This provides functional evidence of a ...
Based on homology with GLUT1-5, we have isolated a cDNA for a novel glucose transporter, GLUTX1. This cDNA encodes a protein of 478 amino acids that shows between 29 and 32% identity with rat GLUT1-5 and 32-36% identity with plant and bacterial hexose transporters. Unlike GLUT1-5, GLUTX1 has a short extracellular loop between transmembrane domain (TM) 1 and TM2 and a long extracellular loop between TM9 and TM10 that contains the only N-glycosylation site. When expressed in Xenopus oocytes, GLUTX1 showed strong transport activity only after suppression of a dileucine internalization motif present in the amino-terminal region. Transport activity was inhibited by cytochalasin B and partly competed by D-fructose and D-galactose. The Michaelis-Menten constant for glucose was approximately 2 mM. When translated in reticulocytes lysates, GLUTX1 migrates as a 35-kDa protein that becomes glycosylated in the presence of microsomal membranes. Western blot analysis of GLUTX1 transiently expressed
Insulin stimulates glucose transport in isolated fat cells by activation of glucose transporters in the plasma membranes and through translocation of the glucose transporter sub-types GLUT4 (insulin-regulatable) and GLUT1 (HepG2 transporter). The protein kinase C-stimulating phorbol ester phorbol 12-myristate 13-acetate (PMA) is able to mimic partially the effect of insulin on glucose transport, apparently through stimulation of carrier translocation. In order to ascertain whether protein kinase C is involved in the translocation signal to both carrier sub-types, we determined the effect of PMA on the subcellular distribution of GLUT1 and GLUT4 by immunoblotting with specific antibodies directed against these transporters. Isolated rat fat cells (4 x 10(6) cells/ml) were stimulated for 20 min with insulin (6 nM) or PMA (1 nM). 3-O-Methylglucose transport was determined and plasma membranes and low-density microsomes were prepared for Western blotting. 3-O-Methylglucose transport was stimulated ...
Articular cartilage is an avascular connective tissue in which the availability of oxygen and glucose is significantly lower than synovial fluid and plasma. Glucose is an important metabolic fuel and structural precursor that plays a key role in the synthesis of extracellular matrix macromolecules in articular cartilage. However, glucose concentrations in cartilage can fluctuate depending on age, physical activity and endocrine status. Chondrocytes are glycolytic cells and must be able to sense the quantities of oxygen and glucose available to them in the extracellular matrix and respond appropriately by adjusting cellular metabolism. Consequently chondrocytes must have the capacity to survive in an extracellular matrix with limited nutrients and low oxygen tensions. The molecular mechanisms responsible for allowing chondrocytes to adapt to these harsh environmental conditions are poorly understood. In this article we present a novel dual model of oxygen and glucose sensing in chondrocytes ...
Several studies have demonstrated that the intrinsic catalytic activity of cell surface glucose transporters is highly regulated in 3T3-L1 adipocytes expressing GLUT1 (erythrocyte/brain) and GLUT4 (adipocyte/skeletal muscle) glucose transporter isoforms. For example, inhibition of protein synthesis in these cells by anisomycin or cycloheximide leads to marked increases in hexose transport without a change in the levels of cell surface glucose transporter proteins (Clancy, B. M., Harrison, S. A., Buxton, J. M., and Czech, M. P. (1991) J. Biol. Chem. 266, 10122-10130). In the present work the exofacial hexose binding sites on GLUT1 and GLUT4 in anisomycin-treated 3T3-L1 adipocytes were labeled with the cell-impermeant photoaffinity reagent [2-3H]2-N-[4-(1-azitrifluoroethyl)benzoyl]-1,3-bis- (D-mannos-4-yloxy)-2-propylamine [( 2-3H] ATB-BMPA) to determine which isoform is activated by protein synthetic blockade. As expected, a 15-fold increase in 2-deoxyglucose uptake in response to insulin was associated
We demonstrated the specificity of the GLUT4 antagonist, indinavir, in cultured cells and observed that inhibition of GLUT4 in endothelium-denuded mouse aortas reduced basal glucose uptake by approximately half, supporting the conclusion that GLUT4 is a major glucose transporter that participates in basal, as well as in insulin-stimulated, glucose uptake in VSMCs in vivo. We also found that GLUT4 and other glucose transporters specifically and differentially contribute to VSMC contraction. Moreover, this differential contribution to contraction varies depending on the contractile agonist. We also observed that GLUT4 expression in vessels from hypertensive animals was diminished, and that indinavir caused a less profound attenuation of maximal 5-HT-mediated contraction in these vessels. We have found that chronic knockout of the GLUT4 gene results in augmented arterial reactivity to the same agonists that elicited attenuated reactivity in response to indinavir. Similar increases in reactivity ...
PI3-kinase signal transduction in diabetes and cancer; molecular mechanisms of cancer cell metastasis and radiation/chemotherapy resistance; tumor suppressor genes with emphasis on NHERF scaffold proteins; transgenic mouse models Current studies in our lab focus on: -Identification of molecular signals targeting the activity and surface expression of insulin-sensitive glucose transport proteins (phosphorylation, ubiquitination, endosomal recycling) -Functional characterization and localization of facilitative glucose transporter GLUT12 in tumor cells -Role of nutrient (glucose) uptake in decreased sensitivity to radiation and chemotherapy: contribution of glucose transporters to radiation survival, glucose transporter expression levels as a predictive marker of radioresistance -Significance of NHERF/Akt/SGK interaction in cancer cell growth and metastasis -Generation of transgenic mouse ...
250 µCi quantities of 2-[14C(U)]-Deoxy-D-Glucose (300-350mCi/mmol) are available for your research. Application of [14C]Deoxy-D-Glucose can be found in: glucose transporter isoform GLUT4 gene regulation and mechanisms in insulin resistance, selectively suppressing the quinolinic acid-induced enhancement of anaerobic glycolysis in glial cells, stimulatory effect of d-ephedrine on ß3-adrenoceptors in adipose tissue of rats, glucose utilization in the brain during acute seizure as a useful biomarker for the evaluation of anticonvulsants, etc. ...
250 µCi quantities of 2-[14C(U)]-Deoxy-D-Glucose (300-350mCi/mmol) are available for your research. Application of [14C]Deoxy-D-Glucose can be found in: glucose transporter isoform GLUT4 gene regulation and mechanisms in insulin resistance, selectively suppressing the quinolinic acid-induced enhancement of anaerobic glycolysis in glial cells, stimulatory effect of d-ephedrine on ß3-adrenoceptors in adipose tissue of rats, glucose utilization in the brain during acute seizure as a useful biomarker for the evaluation of anticonvulsants, etc. ...
We used transtrophectodermal 3-O- methyl glucose flux studies and also polyclonal antibodies raised against either the human erythrocyte glucose transport protein or an artificial peptide homologous to the rat brain glucose transporter C-terminus to characterize and localize the glucose transporter system in the rabbit embryon.
TY - JOUR. T1 - Immunoelectron microscopic demonstration of insulin-stimulated translocation of glucose transporters to the plasma membrane of isolated rat adipocytes and masking of the carboxyl-terminal epitope of intracellular GLUT4. AU - Smith, Robert M.. AU - Charron, Maureen J.. AU - Shah, Neelima. AU - Lodish, Harvey F.. AU - Jarett, Leonard. PY - 1991/8/1. Y1 - 1991/8/1. N2 - Polyclonal antibodies to the amino- or carboxyl-terminal peptide sequences of the GLUT4 transporter protein were used in immunoelectron microscopic studies to demonstrate the location and insulin-induced translocation of GLUT4 in intact isolated rat adipocytes. Labeling of untreated adipocytes with the amino-terminal antibody revealed 95% of GLUT4 was intracellular, associated with plasma membrane invaginations or vesicles contiguous with or within 75 nm of the cell membrane. Insulin treatment increased plasma membrane labeling ≈13-fold, to 52% of the total transporters, and decreased intracellular labeling ...
Complementary DNA encoding a facilitative glucose transporter was isolated from a human hepatoma cell line (HepG2) cDNA library and subcloned into a metal-inducible mammalian expression vector, pLEN (California Biotechnology) containing human metallothionein gene II promoter sequences. Chinese hamster ovary (CHO) cells transfected with this transporter expression vector, pLENGT, exhibited a 2-17-fold increase in immunoreactive HepG2-type glucose transporter protein, as measured by protein immunoblotting with antipeptide antibodies directed against the HepG2-type glucose transporter C-terminal domain. Expression of the human glucose transporter was verified by protein immunoblotting with a mouse polyclonal antiserum that recognizes the human but not the rodent HepG2-type transporter. 2-Deoxy-D-glucose uptake was increased 2-7-fold in transfected cell lines. Polyclonal antisera directed against purified red blood cell glucose transporter were raised in several rabbits. Antiserum from one rabbit, delta,
The subject invention concerns materials and methods for treating oncological disorders in a person or animal using any agent or compound that inhibits uptake of glucose into a cell. The subject invention also concerns methods for inducing apoptosis and inhibiting the proliferation or survival of a cell. In one embodiment, the methods comprise administering an effective amount of an agent or compound that inhibits the activity of one or more glucose transporter proteins, such as Glut-1. An antibody that binds to and inhibits a glucose transporter protein can be used in the present methods.
TY - JOUR. T1 - 4F2hc stabilizes GLUT1 protein and increases glucose transport activity. AU - Ohno, Haruya. AU - Nakatsu, Yusuke. AU - Sakoda, Hideyuki. AU - Kushiyama, Akifumi. AU - Ono, Hiraku. AU - Fujishiro, Midori. AU - Otani, Yuichiro. AU - Okubo, Hirofumi. AU - Yoneda, Masayasu. AU - Fukushima, Toshiaki. AU - Tsuchiya, Yoshihiro. AU - Kamata, Hideaki. AU - Nishimura, Fusanori. AU - Kurihara, Hiroki. AU - Katagiri, Hideki. AU - Oka, Yoshitomo. AU - Asano, Tomoichiro. N1 - Copyright: Copyright 2011 Elsevier B.V., All rights reserved.. PY - 2011/5. Y1 - 2011/5. N2 - Glucose transporter 1 (GLUT1) is widely distributed throughout various tissues and contributes to insulin- independent basal glucose uptake. Using a split-ubiquitin membrane yeast two-hybrid system, we newly identified 4F2 heavy chain (4F2hc) as a membrane protein interacting with GLUT1. Though 4F2hc reportedly forms heterodimeric complexes between amino acid transporters, such as LAT1 and LAT2, and regulates amino acid uptake, ...
Abstract. Diabetes mellitus (DM) is a metabolic diseases characterized by hyperglycemia due to insufficient or inefficient insulin secretory response. This chronic disease is a global problem and there is a need for greater emphasis on therapeutic strategies in the health system. Phytochemicals such as flavonoids have recently attracted attention as source materials for the development of new antidiabetic drugs or alternative therapy for the management of diabetes and its related complications. The antidiabetic potential of flavonoids are mainly through their modulatory effects on glucose transporter by enhancing GLUT-2 expression in pancreatic β cells and increasing expression and promoting translocation of GLUT-4 via PI3K/AKT, CAP/Cb1/TC10 and AMPK pathways. This review highlights the recent findings on beneficial effects of flavonoids in the management of diabetes with particular emphasis on the investigations that explore the role of these compounds in modulating glucose transporter ...
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Glucose Transporter GLUT6兔多克隆抗体(ab119272)可与人样本反应并经WB, ICC/IF实验严格验证。所有产品均提供质保服务,中国75%以上现货。
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An accelerated rate of glucose transport is among the most characteristic biochemical markers of cellular transformation. To study the molecular mechanism by which transporter activity is altered, cultured rodent fibroblasts transfected with activated myc, ras, or src oncogenes were used. In myc-transfected cells, the rate of 2-deoxy-D-glucose uptake was unchanged. However, in cells transfected with activated ras and src oncogenes, the rate of glucose uptake was markedly increased. The increased transport rate in ras- and src-transfected cells was paralleled by a marked increase in the amount of glucose transporter protein, as assessed by immunoblots, as well as by a markedly increased abundance of glucose transporter messenger RNA. Exposure of control cells to the tumor-promoting phorbol ester 12-O-tetradecanoyl phorbol-13-acetate (TPA) for 18 hours had a similar effect of increasing the rate of glucose transport and the abundance of transporter messenger RNA. For ras, src, and TPA, the ...
Type 2 diabetes (T2D) can be viewed as a failure of homeostatic mechanisms that promote nutrient turnover and storage in response to hormonal cues. Although the factors that favor disease progression are heterogeneous, evidence from prospective human studies indicates that impairment of insulin-dependent glucose uptake and utilization is an early event in disease pathogenesis (1). The largest fraction of insulin-dependent glucose disposal (∼70%) occurs in skeletal muscle and is mediated by the insulin-responsive glucose transporter Glut4 (2). A quantitatively smaller contribution (5-20%) is provided by adipose tissue (3). That skeletal muscle is an important site of insulin resistance in humans and that impaired insulin action in muscle leads to adaptive changes in nutrient use from carbohydrates to lipids and to compensatory β-cell hyperplasia are beyond dispute (4). Similarly, insulin resistance in adipose tissue is contributory to the pathogenesis of diabetes not only through impaired ...
In skeletal muscle, acute insulin treatment results in the recruitment of the GLUT4 glucose transporter from intracellular vesicular structures to the plasma membrane. The precise nature of these intracellular GLUT4 stores has, however, remained poorly defined. Using an established skeletal-muscle fractionation procedure we present evidence for the existence of two distinct intracellular GLUT4 compartments. We have shown that after fractionation of crude muscle membranes on a discontinuous sucrose gradient the majority of the GLUT4 immunoreactivity was largely present in two sucrose fractions (30 and 35%, w/w, sucrose; denoted F30 and F35 respectively) containing intracellular membranes of different buoyant densities. Here we show that these fractions contained 44±6 and 49±7% of the crude membrane GLUT4 reactivity respectively, and could be further discriminated on the basis of their immunoreactivity against specific subcellular antigen markers. Membranes from the F30 fraction were highly ...
The oxidation of glucose represents a major source of metabolic energy for mammaliancells. However, because the plasma membrane is impermeable to polar molecules such as glucose, the cellular uptake of this important nutrient is accomplished by membrane-associated carrier proteins that bind and transfer it across the lipid bilayer. Two classes of glucose carriers have been described in mammalian cells: the Na+-glucose cotransporter and the facilitative glucose transporter. The Na+-glucose cotransporter transports glucose against its concentration gradient by coupling its uptake with the uptake of Na+ that is being transported down its concentration gradient. Facilitative glucose c rriers accelerate the transport of glucose down its concentration gradient by facilitative diffusion, a form of passive transport. cDNAs have been isolated from human tissues encoding a Na+-glucose-cotransporter protein and five functional facilitative glucosetransporter isoforms. The Na+-glucose cotransporter is ...
S. cerevisiae has membrane proteins that act as glucose receptors. Glucose binds to these receptors and generates an intracellular signal. In the Rgt2/Snf3 pathway, these two proteins act as glucose receptors. The Rgt2 and Snf3 proteins resemble hexose transporters in structure but have long cytoplasmic tails that are required for signal transduction [7]. Glucose binding to these transmembrane proteins initiates signals that activate a pathway that allows hexose transporter gene expression by repressing Rgt1 function [8].. An additional pathway that involves transcriptional changes in response to glucose is the stimulation of adenylyl cyclase and the increase in intracellular cyclic AMP. This pathway includes a G-protein coupled receptor (Gpr1) and two G proteins Gpa1 and 2, necessary for the glucose-specific increase in cAMP [9,10]. Finally, glucose activation of adenylyl cyclase leads to activation of the cAMP-dependent protein kinase A (PKA). Upon activation of PKA by cAMP the Rap1 ...
ISHIKAWA Nobuhisa , OGURI Tetsuya , ISOBE Takeshi , FUJITAKA Kazunori , KOHNO Nobuoki Japanese journal of cancer research : gann 92(8), 874-879, 2001-08-31 医中誌Web 参考文献32件 ...
Membrane transport in cells is a fundamental biological process that is mediated by various channel and transporter proteins. A major type of such proteins is secondary active membrane transporters, which use a solute gradient to drive the translocation of other substrates. The largest secondary transporter protein family known is the major facilitator superfamily (MFS), with more than one hundred thousand members identified to date. These proteins transport ions, sugars, sugar-phosphates, drugs, neurotransmitters, nucleosides, amino acids, peptides, and other hydrophilic solutes. Members of this superfamily are ubiquitous in all three kingdoms of living organisms, and many have medical or pharmacological relevance. For example, the mammalian glucose transporter Glut4 from muscle and adipose cells is responsible for their glucose uptake, a process that is impaired in type II diabetes. Inherited mutations in a related transporter, Glut1 from erythrocyte and brain blood barrier, cause Glucose ...
The human glucose transporter GLUT1 is abundant in red blood cells, the blood-brain barrier and epithelial cells, where it mediates the transport of the energy metabolite, glucose. In the present work some properties of GLUT1, including affinity binding of both substrates and inhibitors, transport rates as well as permeabilities of aromatic amino acids and drug-membrane interactions were analyzed by chromatographic methods.. Reconstitution by size-exclusion chromatography on Superdex 75 from a detergent with a low CMC that provides monomeric GLUT1 was examined regarding D-glucose- and CB binding as well as D-glucose transport. Upon steric immobilization in Superdex 200 gel beads, residual detergent could be washed away and dissociation constants in the same range as reported for binding to GLUT1 reconstituted from other detergents were obtained. The transport rate into the GLUT1 proteoliposomes was low, probably due to residual detergent. Binding to GLUT1 at different pH was analyzed and the ...
GLUT5 antibody (solute carrier family 2 (facilitated glucose/fructose transporter), member 5) for WB. Anti-GLUT5 pAb (GTX47818) is tested in Human samples. 100% Ab-Assurance.
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One hallmark of cancer is the accelerated metabolism, high energy requirements, and increased glucose uptake by the tumor cells, the latter being the first and rate-limiting step for glucose metabolism. Glucose transport into the tumor cell is mediated by facilitative high-affinity glucose transporter (GLUT) proteins. Among the 14 GLUT proteins, expression of GLUT1 in normal organs is nearly exclusively restricted to the blood brain barrier, while other GLUTs are also expressed in a wide variety of vital organs such as liver and heart. Interestingly, GLUT1 expression is highly regulated by hypoxia-inducible factor (HIF)-1α, a key driver of tumor progression. In line with this finding, GLUT1 over-expression was found to be associated with tumor progression and poor overall survival in various tumor indications. Consequently, GLUT1 represents a potential target for cancer treatment. Therefore, we have developed a highly-selective GLUT1 inhibitor, namely BAY-876, with selectivity over GLUT2, 3, ...
Glucose Transporter Inhibitor IV, WZB117 - CAS 1223397-11-2 - Calbiochem Glucose Transporter Inhibitor IV, WZB117, CAS 1223397-11-2, is a fast-acting, irreversible blocker of GLUT1 in RBCs. Also inhibits glucose transport in cancer cells (IC50 ~ 500 nM in A549 cells). - Find MSDS or SDS, a COA, data sheets and more information.
GLUT4 is the major glucose transporter in skeletal muscle. GLUT4 cycles to and from the plasma membrane and its exocytic rate is accelerated by insulin and muscle contraction to achieve a new steady state with more GLUT4 proteins at the muscle cell surface. To gain a better understanding of the molecular and cellular mechanisms that govern GLUT4 protein recycling, we developed an in vitro model in which myc-epitope-tagged GLUT4 or GLUT4-GFP is expressed in L6 skeletal muscle cells. The myc-epitope is inserted into an exofacial domain that is accessible to anti-myc antibodies from the outside of non-permeabilized cells, allowing one to count the number of transporters at the cell surface ...
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Facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney ...
One of the most important metabolic actions of insulin is catalysing glucose uptake into skeletal muscle and adipose tissue. This is accomplished via activation of the PI3K/Akt signalling pathway and subsequent translocation of GLUT4 from intracellular storage vesicles to the plasma membrane. As such, this represents an ideal system for studying the convergence of signal transduction and protein trafficking. The GLUT4 translocation process is complex, but can be dissected into at least 4 discrete trafficking steps. This raises the question as to which of these is the major regulated step in insulin-stimulated GLUT4 translocation. Numerous molecules have been reported to regulate GLUT4 trafficking. However, with the exception of TBC1D4, the molecular details of these distal signalling arms of the insulin signalling network and how they modify distinct steps of GLUT4 trafficking have not been established. We discuss the need to adopt a more global approach to expand and deepen our understanding of the
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The protein's function is to transport monosaccharide sugars into these sink tissues. The protein is classed as a symporter ... Sodium-glucose transport proteins Glucose transporter Truernit E, Schmid J, Epple P, Illig J, Sauer N (December 1996). "The ... Stp4 (sugar transporter protein 4) is a gene from the model plant, Arabidopsis thaliana. The gene transcribes for an integral ... Fotopoulos V, Gilbert MJ, Pittman JK, Marvier AC, Buchanan AJ, Sauer N, Hall JL, Williams LE (June 2003). "The monosaccharide ...
... monosaccharide - monosaccharide transport protein - morphogenesis - morphogenetic field - mos gene - Mössbauer spectroscopy - ... protein - protein biosynthesis - Protein Data Bank - protein design - protein expression - protein folding - protein isoform - ... protein P16 - protein P34cdc2 - protein precursor - protein structure prediction - protein subunit - protein synthesis - ... proto-oncogene protein C-kit - proto-oncogene proteins c-abl - proto-oncogene proteins c-bcl-2 - Proto-oncogene proteins c-fos ...
Saier MH Jr (1998). "Molecular phylogeny as a basis for the classification of transport proteins from bacteria, archaea and ... In enzymology, a monosaccharide-transporting ATPase (EC 3.6.3.17) is an enzyme that catalyzes the chemical reaction ATP + H2O ... and monosaccharide, whereas its 3 products are ADP, phosphate, and monosaccharide. This enzyme belongs to the family of ... The systematic name of this enzyme class is ATP phosphohydrolase (monosaccharide-importing). This enzyme participates in abc ...
... permease or GalP found in Escherichia coli is an integral membrane protein involved in the transport of monosaccharides, ... Transmembrane protein List of proteins (Integral membrane proteins, Bacterial proteins). ... GalP is a monosaccharide transporter that uses a chemiosmotic mechanism to transport its substrates into the cytoplasm of E. ... It transports these sugars at faster rates with a proton gradient but can still transport them in a leaky fashion without a ...
The monosaccharides and amino acids thus produced are subsequently transported across the intestinal epithelium and eventually ... These interactions include those between proteins in the same membrane ("cis") and proteins in adjacent cells ("trans"). In ... They are formed by interactions between intracellular adapter proteins, transmembrane proteins and the actin cytoskeletons of ... the intracellular domains of tight junctions interact with different scaffold proteins, adapter proteins and signaling ...
Protein is hydrolysed to peptides and amino acids by microbial enzymes, which are subsequently transported across the microbial ... proteins, peptides, and amino acids). Both non-structural and structural carbohydrates are hydrolysed to monosaccharides or ... Protein encoding genes that encode for bacterial cell functions, such as aguA, ptb, K01188, and murD, also are associated with ... In situations in which nitrogen for microbial growth is in excess, protein and its derivatives can also be fermented to produce ...
For example, cytochalasin A and cytochalasin B can also inhibit the transport of monosaccharides across the cell membrane, ... cytochalasin H has been found to regulate plant growth, cytochalasin D inhibits protein synthesis and cytochalasin E prevents ... "Cytochalasin Releases mRNA from the Cytoskeletal Framework and Inhibits Protein Synthesis". Molecular and Cellular Biology. 6 ( ...
Proteins are also important in cell signaling, immune responses, cell adhesion, active transport across membranes, and the cell ... Monosaccharides can be linked together to form polysaccharides in almost limitless ways. The two nucleic acids, DNA and RNA, ... This is done in eukaryotes by a series of proteins in the membranes of mitochondria called the electron transport chain. In ... The amino acids or sugars released by these extracellular enzymes are then pumped into cells by active transport proteins. ...
... is a dominant monosaccharide in N-linked glycosylation, which is a post-translational modification of proteins. It is ... The PEP-dependent sugar transporting phosphotransferase system transports and simultaneously phosphorylates its sugar ... Recombinant proteins produced in yeast may be subject to mannose addition in patterns different from those used by mammalian ... to nascent glycoproteins in the endoplasmic reticulum in a co-translational manner as the protein entered through the transport ...
Member 5, also known as SLC17A5 or sialin is a lysosomal membrane sialic acid transport protein which in humans is encoded by ... A multiple lysosomal transport defect for acidic monosaccharides". J. Clin. Invest. 87 (4): 1329-35. doi:10.1172/JCI115136. PMC ... Sialin, also known as H(+)/nitrate cotransporter and H(+)/sialic acid cotransporter, is a protein which in humans is encoded by ... A deficiency of this protein causes Salla disease. and Infantile Sialic Acid Storage Disease (ISSD). The gene for HP59 contains ...
How do transport vesicles know the final destination of the protein that they are transporting? Vesicles are directed by many ... O-linked glycosylation occurs in the Golgi apparatus, where monosaccharide units are added to a complete polypeptide chain. ... Cell surface proteins and extracellular proteins are O-glycosylated. Glycosylation sites in O-linked oligosaccharides are ... ensuring that the protein is transported to the appropriate destination. Many cells produce specific carbohydrate-binding ...
An astounding 8% of protein coding genes are related to carbohydrate transport in C. indolis. These genes are placed into two ... PTS transporters carry a wide variety of monosaccharides and disaccharides, and have a high affinity for hexose. C. indolis ... it was found that it contains an abundance of genes in functional groups associated with the transport and utilization of ...
The transport of ammonium and amino acids from fungus to plant is also regulated. Nitrogen is essential in plant biochemistry, ... For example, monosaccharide uptake in Amanita muscaria requires a transporter that is only expressed when it is in a ... Some polypeptides are only found when the fungus and plant have achieved symbiosis; these symbiosis-related (SR) proteins are ... 1994). "Nutrient transport in mycorrhizas: structure, physiology and consequences for efficiency of the symbiosis" (PDF). Plant ...
Other areas of biochemistry include the genetic code (DNA, RNA), protein synthesis, cell membrane transport, and signal ... carbohydrates are formed from sugars known as monosaccharides, oligosaccharides, and polysaccharides, lipids are formed from ... "The restrictions on possible mechanisms of intestinal transport of sugars". In: Membrane Transport and Metabolism. Proceedings ... argued that proteins were merely carriers for the true enzymes and that proteins per se were incapable of catalysis. However, ...
In creature tissues, the antecedent glucosylceramide is moved by the sphingolipid transport protein FAPP2 to the distal Golgi, ... In creature tissues, biosynthesis of lactosylceramide includes expansion of the second monosaccharides unit (galactose) as its ... Biosynthesis of lactosylceramide then includes expansion of the second monosaccharides unit as its actuated nucleotide ...
Membrane transport protein disorders, Rare diseases). ... or monosaccharides. Sucrose and lactose are called ... The SLC5A1 gene provides instructions for producing a sodium/glucose cotransporter protein called SGLT1. This protein is found ... The sodium/glucose cotransporter protein is involved in the process of glucose uptake in the instesinal cells due to a sodium ... In addition, the water that normally would have been transported across the brush border with the sugar instead remains in the ...
The monosaccharide units can then enter into monosaccharide catabolism. A 2 ATP investment is required in the early steps of ... Pichon L, Huneau JF, Fromentin G, Tomé D (May 2006). "A high-protein, high-fat, carbohydrate-free diet reduces energy intake, ... Sucrose, pictured to the right, is the most abundant disaccharide, and the main form in which carbohydrates are transported in ... The smallest monosaccharides, for which n=3, are dihydroxyacetone and D- and L-glyceraldehydes. Monosaccharides are classified ...
... leading to increased transport proteins. High-fructose diets (>2.4 g/kg body wt) increase transport proteins within three days ... All three dietary monosaccharides are transported into the liver by the GLUT2 transporter. Fructose and galactose are ... assisted by transport proteins. Fructose may be transported out of the enterocyte across the basolateral membrane by either ... the claim that fructose absorption occurs on the mucosal membrane via facilitated transport involving GLUT5 transport proteins ...
... which are able to be transported into the periplasm of Planctomycetota by specialized proteins. The third hypothesis involves ... degrades the complex substrates into smaller monosaccharides, which can more easily be transported through the different ... FtsZ proteins are suggested to be similar in structure to that of tubulin, the protein present in eukaryotes, and is essential ... Gained protein families in Gemmataceae, a subgroup within Planctomycetota, have low sequence similarity to eukaryotic proteins ...
... is subsequently transported to the Golgi apparatus by either vesicular trafficking or the ceramide transfer protein ... In the case of glycosphingolipids, exohydrolases acting at acidic pH optima cause the stepwise release of monosaccharide units ... This leads to the egress of proteins from the intermembrane space. Ceramides may be found as ingredients of some topical skin ... Davis, Deanna; Kannan, Muthukumar; Wattenberg, Binks (2018-12-01). "Orm/ORMDL proteins: Gate guardians and master regulators". ...
Specializing occurs when the digestive tract or alimentary canal has a separate mouth and anus so that transport of food is one ... Chemical digestion involves hydrolysis reactions that liberate the sub unit molecules-primarily monosaccharides, amino acids ... and proteins into their smallest sub-units. ... transport, osmotrophy or phagocytosis. Since digestion occurs ...
IPTG is an effective inducer of protein expression in the concentration range of 100 μmol/L to 3.0 mmol/L. Typically, a sterile ... IPTG uptake by E. coli can be independent of the action of lactose permease, since other transport pathways are also involved. ... a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. But unlike allolactose, the sulfur (S ... At low concentration, IPTG enters cells through lactose permease, but at high concentrations (typically used for protein ...
... glycan-binding proteins, glycan degradation proteins, intercellular protein transport proteins, sugar transporters, adhesion ... monosaccharides, disaccharides, etc.), glycan-binding proteins, and anti-glycan antibodies for distribution to investigators. ... Protein-Glycan Interaction Core (H), located at Emory University, analyzes investigator-generated lectins, antibodies, antisera ... From 2001-2009, Core F generated 26 total and conditional knockout mouse lines deficient in glycan-binding proteins or ...
... starch proteins: primary structure - secondary structure - tertiary structure - conformation - native state - protein folding ... cilium Cell transport: Diffusion - Osmosis - isotonic - active transport - phagocytosis Cellular reproduction: cytokinesis - ... monosaccharide - amino acids - nucleotide - functional group - monomer - adenosine triphosphate (ATP) - lipids - oil - sugar - ... Molecular neuroscience - studies the biology of the nervous system with molecular biology, molecular genetics, protein ...
Monosaccharides commonly found in eukaryotic glycoproteins include:: 526 The sugar group(s) can assist in protein folding, ... P-glycoprotein, or multidrug transporter (MDR1), is a type of ABC transporter that transports compounds out of cells. This ... Secreted extracellular proteins are often glycosylated. In proteins that have segments extending extracellularly, the ... covalently linked to protein. The carbohydrate may be in the form of a monosaccharide, disaccharide(s). oligosaccharide(s), ...
... glucose is the most abundant natural monosaccharide because it is less glycated with proteins than other monosaccharides. ... In other cells, uptake happens by passive transport through one of the 14 GLUT proteins. In the other cell types, ... Unlike for glucose, there is no transport protein for glucose-6-phosphate. Gluconeogenesis allows the organism to build up ... "Reaction of monosaccharides with proteins: possible evolutionary significance". Science. 213 (4504): 222-24. Bibcode:1981Sci... ...
Dang L, Van Damme EJ (September 2015). "Toxic proteins in plants". Phytochemistry. 117: 51-64. doi:10.1016/j.phytochem.2015.05. ... synthesized from tryptophan is a component of the coenzymes NAD+ and NADP+ which in turn are required for electron transport in ... monosaccharides → polysaccharides (cellulose, chitin, glycogen etc.) Acetate pathway → fatty acids and polyketides Shikimate ... These first messengers interact with cellular receptors which are composed of proteins. Cellular receptors in turn activate ...
... combined and rearranged to form monosaccharide sugars, such as glucose, which can be transported to other cells, or packaged ... Click on genes, proteins and metabolites below to link to respective articles. [[File ...
Breaking down a protein into amino acids, or a triglyceride into fatty acids, or a disaccharide into monosaccharides are all ... The ribose-5-phosphate can be transported into the nucleic acid metabolism, producing the basis of DNA and RNA monomers, the ... It provides electrons to the electron transport chain which is used to drive the production of ATP in oxidative phosphorylation ... These include the formation of carbohydrates, proteins, lipids and nucleic acids. The second are reduction reactions, in which ...
Some can inhibit a specific function of a protein or disrupt protein-protein interactions. Pharmacology usually restricts the ... and monosaccharides, respectively) are often considered small molecules. Small molecules may be used as research tools to probe ... weight cutoff is also a necessary but insufficient condition for oral bioavailability as it allows for transcellular transport ... Arkin MR, Wells JA (April 2004). "Small-molecule inhibitors of protein-protein interactions: progressing towards the dream". ...
Allosteric inhibition and activation by Protein-protein interactions (PPI).[28] Indeed, some proteins interact with and ... The intra-mitochondrial NADH + H+ is oxidized to NAD+ by the electron transport chain, using oxygen as the final electron ... Metabolism of common monosaccharides, including glycolysis, gluconeogenesis, glycogenesis and glycogenolysis History[edit]. The ... PFK2 is phosphorylated by protein kinase A. The phosphorylation inactivates PFK2, and another domain on this protein becomes ...
Protein builds tissue and cells in the body. Carbohydrates are very good for energy, but, if a person eats more than needed, ... "Monosaccharides - an overview , ScienceDirect Topics". www.sciencedirect.com. Retrieved June 3, 2021.. ... Certain carbohydrates are an important storage and transport form of energy in most organisms, including plants and animals. ... If necessary, humans can live without eating carbohydrates because the human body can change fats and proteins into ...
TransportEdit. This section is an excerpt from Petroleum transport.[edit]. Petroleum transport is the transportation of ... Monosaccharides in turn ultimately decayed to CO2 and methane. The anaerobic decay products of amino acids, monosaccharides, ... Due to such anaerobic bacteria, at first this matter began to break apart mostly via hydrolysis: polysaccharides and proteins ... In 2018 road transport used 49% of petroleum, aviation 8%, and uses other than energy 17%.[151] Electric vehicles are the main ...
Trypsin inhibitors decrease the digestion of the protein. Lectins can impede the uptake of glucose, decrease nutrient transport ... and vicilin proteins are trimers. Pea seeds contain 60-65% carbohydrates mainly composed of oligosaccharides, monosaccharides, ... Pea protein is rich in nutrients such as protein and carbohydrates. Pea protein also contains vitamins and minerals and is low ... Pea protein can be used as a protein supplement to increase muscle mass. Increasing protein intake creates a positive acute ...
... s can be proteins, peptides (amino acid chains), polysaccharides (chains of monosaccharides/simple sugars), lipids, or ... These algorithms consider factors such as the likelihood of proteasomal processing, transport into the endoplasmic reticulum, ... Immunoglobulin-binding protein - Proteins such as protein A, protein G, and protein L that are capable of binding to antibodies ... An autoantigen is usually a self-protein or protein complex (and sometimes DNA or RNA) that is recognized by the immune system ...
Following that, the sap was transported by sled to large fires where it was boiled in clay pots to produce maple syrup. Often, ... It consists primarily of sucrose and water, with small amounts of the monosaccharides glucose and fructose from the invert ... and no appreciable protein or fat (table). Maple syrup is generally low in overall micronutrient content, although manganese ... The harvested sap was transported back to the party's base camp, where it was then poured into large vessels (usually made from ...
Across proteins, the active site entails an α-β barrel with either an arginine or tryptophan residues in the barrel pocket to ... During the duration of the molting periods, these monomers are continually transported by vesicles closer to the epidermis, ... and specific monosaccharide products include N-acetyl-d-glucosamine and N-acetyl-d-galactosamine. N-acetyl-β-d-glucosaminidase ... The same reaction catalyzed by proteins isolated from calf brain was generated at an observed Km of 0.72 mM, with a maximum ...
Protein a class of biochemicals made from amino acids in specific sequences. Proteins can be very large molecules with very ... It is also involved in the transport of fat (i.e., lipids) in the blood. The transport mechanism (Low Density Cholesterol or ... Only a few of the simple sugars (mono-saccharides) and even fewer of the di-saccharides (e.g., lactose) in food are available ... Proteinuria presence of protein in urine, and evidence of serious malfunction of the kidneys as protein is normally very ...
Mean ± SEM activity in nmol/min per mg protein § = 12 rats/group * = Significantly different from control at p < 0.05 The lack ... Fructose is a dietary monosaccharide present naturally in fruits and vegetables, either as free fructose or as part of the ... but it is not transported into cells via insulin-sensitive pathways (insulin regulated transporters GLUT1 and GLUT4). Instead, ... "GLUT5 expression and fructose transport in human skeletal muscle". Advances in Experimental Medicine and Biology. 441: 35-45. ...
This is due to the tendency of tannins to react with proteins, such as the ones found in saliva. In food and wine pairing, ... However, there is evidence that in some plants flavonoids play a role as endogenous regulators of auxin transport. They are ... 247-264, INIST:16184447 Identification of the origin of commercial enological tannins by the analysis of monosaccharides and ... June 2001). "Flavonoids act as negative regulators of auxin transport in vivo in arabidopsis". Plant Physiol. 126 (2): 524-35. ...
Spodsberg N, Jacob R, Alfalah M, Zimmer KP, Naim HY (2001). "Molecular basis of aberrant apical protein transport in an ... Monosaccharides, or single sugar units, are absorbed directly into the blood. A deficiency of sucrase may result in ... When disaccharides are consumed, they must be broken down into monosaccharides by enzymes in the intestines before they can be ... "Compound heterozygous mutations affect protein folding and function in patients with congenital sucrase-isomaltase deficiency ...
The GI tract accomplishes this ion pumping using a system of proteins that are capable of active transport, facilitated ... carbohydrates (monosaccharide, disaccharide) proteins lipids Superior mesenteric artery Inferior mesenteric artery Notes at ... The arrangement of these proteins on the apical and basolateral sides of the epithelium determines the net movement of ions and ... then follows the positive charge in the H+ through an open apical channel protein. HCO3− secretion occurs to neutralize the ...
Zhu X, Sen J, Stevens L, Goltz JS, Stein D (Sep 2005). "Drosophila pipe protein activity in the ovary and the embryonic ... as the body lacks the ability to transport fatty acids into the mitochondria to be processed as a fuel source. The disease is ... EC 2.4 transferases that is involved in biosynthesis of disaccharides and polysaccharides through transfer of monosaccharides ... "1aqy Summary". Protein Data Bank in Europe Bringing Structure to Biology. The European Bioinformatics Institute. Retrieved 11 ...
... but transfer from protein to protein, or from protein back to either type of nucleic acid, is impossible and does not occur ... deoxyribose A monosaccharide sugar derived from ribose by the loss of a single oxygen atom. D-deoxyribose, in its pentose ring ... while others rely on the transport of charged molecules by electrophoresis (e.g. western blotting). The transferred molecules ... DNA-binding domain (DBD) DNA-binding protein (DBP) Any polypeptide or protein containing one or more domains capable of ...
The monosaccharide glucose-6-phosphate (G-6-P) is typically the input substance for glycogenesis. G-6-P is most commonly ... During glycolysis ATP, NADH (both an energy transport form used inside cells) as well as pyruvate are produced. Glycolysis is ... PRKAG2 is a noncatalytic gamma subunit of AMP-activated protein kinase (AMPK), which affects the release of G-1-P by ... These carbohydrates are composed of three principal monosaccharides: glucose, fructose and galactose; in addition glycogen is ...
... and of the monosaccharide glucose. In terms of nitrogen-rich sources, saprotrophs require combined protein for the creation of ... The process is most often facilitated through the active transport of such materials through endocytosis within the internal ... Proteins are broken down into their amino acid composites through the breaking of peptide bonds by proteases. Lipids are broken ... proteins, vitamins and in some cases, ions. Due to the carbon composition of the majority of organisms, dead and organic matter ...
Glucose can be transported from the intestines or liver to other tissues in the body via the bloodstream. Cellular glucose ... Levine R (1986). "Monosaccharides in health and disease". Annual Review of Nutrition. 6: 211-24. doi:10.1146/annurev.nu. ... Because red blood cells (erythrocytes) have a higher concentration of protein (e.g., hemoglobin) than serum, serum has a higher ...
Specifically, the HA1 domain of the protein binds to the monosaccharide sialic acid which is present on the surface of its ... the contents of the virus such as viral RNA are released in the host cell's cytoplasm and then transported to the host cell ... By phylogenic similarity, the HA proteins are divided into 2 groups, with H1, H2, H5, H6, H8, H9, H11, H12, H13, H16, H17, and ... Since hemagglutinin is the major surface protein of the influenza A virus and is essential to the entry process, it is the ...
... mediates chemotaxis toward and active transport of glucose and galactose in a number of bacterial species. GGBP, like other ... periplasmic binding proteins, can exist in open (ligand-free) and closed (ligand-bound) states. We report a 0.92 angstroms ... Monosaccharide Transport Proteins / chemistry* * Monosaccharide Transport Proteins / metabolism * Protein Binding * Protein ... D-Glucose/D-Galactose-binding protein (GGBP) mediates chemotaxis toward and active transport of glucose and galactose in a ...
Proteins [D12.776] * Carrier Proteins [D12.776.157] * Membrane Transport Proteins [D12.776.157.530] * Monosaccharide Transport ... Proteins [D12.776] * Membrane Proteins [D12.776.543] * Membrane Transport Proteins [D12.776.543.585] * Monosaccharide Transport ... Proteins [D12.776] * Carrier Proteins [D12.776.157] * Membrane Transport Proteins [D12.776.157.530] * Solute Carrier Proteins [ ... Proteins [D12.776] * Membrane Proteins [D12.776.543] * Membrane Transport Proteins [D12.776.543.585] * Solute Carrier Proteins ...
Proteins, Monosaccharide Transport. Transport Proteins, Hexose. Transport Proteins, Monosaccharide. Transport-Inducing Protein ... Proteins, Monosaccharide Transport Transport Proteins, Monosaccharide Hexose Transport Proteins - Narrower Concept UI. M0014039 ... Erythrocyte Band 4.5 Protein. Glucose Transport Inducing Protein. Glucose Transport-Inducing Protein. Hexose Transport Proteins ... Monosaccharide Transport Proteins - Preferred Concept UI. M0014041. Scope note. A large group of membrane transport proteins ...
Monosaccharide Transport Proteins. *Pancreas. *Receptor Protein-Tyrosine Kinases. *Receptor, Fibroblast Growth Factor, Type 1 ... We generated a transgenic mouse model in which the whey acidic protein (WAP) promoter directs expression of human Fgf4 to ... Fibroblast growth factor 4 precursor (FGF-4) (Heparin secretory-transforming protein 1) (HST) (HST-1) (HSTF-1) (Heparin-binding ... growth factor 4) (HBGF-4) (Transforming protein KS3) [HST] [HSTF1] [KS3] ...
Monosaccharide Transport Proteins 100% * Protein Engineering 80% * Deoxyglucose 68% * Plasmodium falciparum 62% ... The aminoalkylindole bml-190 negatively regulates chitosan synthesis via the cyclic amp/protein kinase a1 pathway in ... falciparum hexose transporter PfHT and assessment of ligand access to the glucose permeation pathway via FLAG-mediated protein ...
Monosaccharide Transport Proteins Medicine & Life Sciences 19% * Genes Medicine & Life Sciences 18% ...
Band 4.5 Preactin use Monosaccharide Transport Proteins Band 6 Protein, Desmosomal use Plakophilins ... Band 3 Anion Transport Protein use Anion Exchange Protein 1, Erythrocyte Band 3 Protein use Anion Exchange Protein 1, ... B-raf, Proto-Oncogene Protein use Proto-Oncogene Proteins B-raf B-raf, Proto-Oncogene Proteins use Proto-Oncogene Proteins B- ... Baculoviral IAP Repeat Containing 2 Protein use Inhibitor of Apoptosis Proteins Baculoviral IAP Repeat Containing 3 Protein use ...
Monosaccharide Transport Proteins. Neurotransmitter Transport Proteins. Nucleobase, Nucleoside, Nucleotide, and Nucleic Acid ...
Monosaccharide Transport Proteins / Muscle Proteins / Receptors, Somatotropin / Time Factors / Transfection. (出版サイトへのリンク). ● ... Mitogen-Activated Protein Kinase 1 / Mitogen-Activated Protein Kinase 3 / Mitogen-Activated Protein Kinases / ... Nerve Tissue Proteins / Organ Specificity / Pancreas / Protein Biosynthesis / Proteins / Sequence Homology, Amino Acid / ... Islet neogenesis-associated protein (INGAP) is a protein expressed during islet neogenesis. We have cloned a novel cDNA having ...
AutoFact: Monosaccharide transport protein 1 n=3 Tax=Andropogoneae RepID=Q6B4G9_MAIZE 0.0 ... AutoFact: sugar transport protein-like [Oryza sativa Japonica Group] dbj,BAD30471.1, sugar transport protein-like [Oryza sativa ... AutoFact: sugar transport protein 14 [Arabidopsis thaliana] ref,NP_001185417.1, sugar transport protein 14 [Arabidopsis ... AutoFact: Mito_carr domain containing protein 1.0e-25 • FL-Next: tr=Putative uncharacterized protein; Vitis vinifera (Grape). ...
Monosaccharide Transport Proteins. * Muscle Proteins. * Nerve Tissue Proteins. * Phosphatidylinositol 3-Kinases. * Wiskott- ...
Biological Transport;Monosaccharide Transport Proteins;Centrifugation, Density Gradient ... Biological Transport;Monosaccharide Transport Proteins;Centrifugation, Density Gradient ... Muscle, Skeletal;Muscle Proteins;Insulin Resistance;Diabetes Mellitus, Type 2;Glucose Transporter Type 4;Cystinyl ... Muscle, Skeletal;Muscle Proteins;Insulin Resistance;Diabetes Mellitus, Type 2;Glucose Transporter Type 4;Cystinyl ...
From there the monosaccharides enter the bloodstream with help of special transport proteins called GLUT transporters. When ... There are 12 transport proteins in all named GLUT1 through GLUT12. All of them facilitate the diffusion of sugars into the ... Monosaccharides and Disaccharides:. Monosaccharides: Are the simplest form of sugar that make up more complex carbohydrates. ... activates the GLUT4 vesicle which stimulates the GLUT4 transport protein in the cell membrane which enables glucose transport. ...
Man6P is a key targeting signal for acid hydrolase precursor proteins that are destined for transport to lysosomes and is ... Release of monosaccharides from the glycoprotein by mild acid hydrolysis.. *Fluorescent labelling of released monosaccharides ... Ludger Glyco-Tools: Quantitative Monosaccharide Analysis. Oxford - June 2020. Monosaccharide analysis is a regulatory ... Relative quantitative analysis of 2AA-labelled monosaccharides by HPLC or UPLC.. Ludger offers a Monosaccharide Release and ...
Protein Synonyms Start End Strand PubMed ID Dde_0261 Dde_0261 Dde_0261 Monosaccharide-transporting ATPase Monosaccharide- ... E) COG411 , ABC-type branched-chain amino acid transport systems, ATPase component ...
polyamine ABC transporter ATP-binding protein. monosaccharide-transporting ATPase (EC 3.6.3.17) (characterized). 39%. 86%. 218 ... Curated sequence Q97UY8: monosaccharide-transporting ATPase (EC 3.6.3.17). Glucose import ATP-binding protein GlcV; EC 7.5.2 ... monosaccharide-transporting ATPase (EC 3.6.3.17) (characterized). 35%. 88%. 208. CP4-6 prophage; ABC transporter ATP-binding ... GapMind searches the predicted proteins for candidates by using ublast (a fast alternative to protein BLAST) to find ...
Glucose is transported into the cells by an active, energy-requiring process that involves a specific transport protein and ... Glucose is a monosaccharide and is the primary metabolite for energy production in the body. Complex carbohydrates are ... GLUT-1 receptors transport glucose across the blood-brain barrier. The rare genetic disease GLUT-1 receptor deficiency ... Glucose enters cells via facilitated transport, mediated by glucose transporter (GLUT) receptors. These receptors demonstrate ...
Band 4.5 Preactin use Monosaccharide Transport Proteins Band 6 Protein, Desmosomal use Plakophilins ... Band 3 Anion Transport Protein use Anion Exchange Protein 1, Erythrocyte Band 3 Protein use Anion Exchange Protein 1, ... B-raf, Proto-Oncogene Protein use Proto-Oncogene Proteins B-raf B-raf, Proto-Oncogene Proteins use Proto-Oncogene Proteins B- ... Baculoviral IAP Repeat Containing 2 Protein use Inhibitor of Apoptosis Proteins Baculoviral IAP Repeat Containing 3 Protein use ...
Band 3 Protein use Anion Exchange Protein 1, Erythrocyte Band 4.5 Preactin use Monosaccharide Transport Proteins ... B-raf, Proto-Oncogene Protein use Proto-Oncogene Proteins B-raf B-raf, Proto-Oncogene Proteins use Proto-Oncogene Proteins B- ... Band 3 Anion Transport Protein use Anion Exchange Protein 1, Erythrocyte ... B Cell Leukemia 2 Family Proteins use Proto-Oncogene Proteins c-bcl-2 ...
Band 4.5 Preactin use Monosaccharide Transport Proteins Band 6 Protein, Desmosomal use Plakophilins ... Band 3 Anion Transport Protein use Anion Exchange Protein 1, Erythrocyte Band 3 Protein use Anion Exchange Protein 1, ... B-raf, Proto-Oncogene Protein use Proto-Oncogene Proteins B-raf B-raf, Proto-Oncogene Proteins use Proto-Oncogene Proteins B- ... Baculoviral IAP Repeat Containing 2 Protein use Inhibitor of Apoptosis Proteins Baculoviral IAP Repeat Containing 3 Protein use ...
Band 4.5 Preactin use Monosaccharide Transport Proteins Band 6 Protein, Desmosomal use Plakophilins ... Band 3 Anion Transport Protein use Anion Exchange Protein 1, Erythrocyte Band 3 Protein use Anion Exchange Protein 1, ... B-raf, Proto-Oncogene Protein use Proto-Oncogene Proteins B-raf B-raf, Proto-Oncogene Proteins use Proto-Oncogene Proteins B- ... Baculoviral IAP Repeat Containing 2 Protein use Inhibitor of Apoptosis Proteins Baculoviral IAP Repeat Containing 3 Protein use ...
The cells in the small intestine have membranes that contain many transport proteins in order to get the monosaccharides and ... The resultant monosaccharides are absorbed into the bloodstream and transported to the liver.. Maintaining Blood Glucose Levels ... As glucose is transported into the cells around the body, the blood glucose levels decrease. Insulin has an opposing hormone ... Insulin sends a signal to the bodys cells to remove glucose from the blood by transporting it into different organ cells ...
Monosaccharide Transport Proteins. * Polymorphism, Restriction Fragment Length. authors with profiles * Susannah Spiess ...
Monosaccharide Transport Proteins. *Muscle Proteins. Rats. Rats, Sprague-Dawley. *Repressor Proteins. *Saccharomyces cerevisiae ... In an attempt to identify proteins present in these structures, GLUT4-enriched vesicles prepared from rat adipocytes treated ... Additionally, immunoadsorbed GLUT4 vesicles were found to catalyze palmitoylation of proteins when incubated with labeled ... Proteins. Life Sciences. Medicine and Health Sciences Show allShow less ...
Carrier Proteins [D12.776.157]. *Membrane Transport Proteins [D12.776.157.530]. *Monosaccharide Transport Proteins [D12.776. ... A glucose transport protein found in mature MUSCLE CELLS and ADIPOCYTES. It promotes transport of glucose from the BLOOD into ... The inactive form of the protein is localized in CYTOPLASMIC VESICLES. In response to INSULIN, it is translocated to the PLASMA ... Membrane Proteins [D12.776.543]. *Membrane Transport Proteins [D12.776.543.585]. *Monosaccharide Transport Proteins [D12.776. ...
Monosaccharide Transport Proteins * Morphogenesis * Multigene Family * Muridae * Muscle, Smooth * Muscle, Smooth, Vascular ... "Biosynthesis and in vivo localization of the decapentaplegic-Vg-related protein, DVR-6 (bone morphogenetic protein-6)." J Cell ... "Effect of protease inhibitors on protein degradation in rat hepatoma cells I. Effect on general protein degradation." Bba ... "Expression of bone morphogenetic protein-4 (BMP-4), bone morphogenetic protein-7 (BMP-7), fibroblast growth factor-8 (FGF-8) ...
... lipid composition of rafts is altered in cell models of Gaucher disease which may be responsible for impaired lipid and protein ... Lipid rafts play a role in facilitating and regulating lipid and protein interactions in cells, and their unique lipid ... Interestingly, this latter cascade is not involved in either insulin stimulated glucose transport or glycogen metabolism [83]. ... monosaccharides are sequentially cleaved by water soluble exohydrolases acting at the non-reducing end sugar in the ...
Monosaccharide Transport Proteins [D12.776.543.585.500] * Neurotransmitter Transport Proteins [D12.776.543.585.562] ... Amino Acids, Peptides, and Proteins [D12] * Proteins [D12.776] * Membrane Proteins [D12.776.543] * Membrane Transport Proteins ... Mitochondrial Membrane Transport Proteins Preferred Concept UI. M0403944. Registry Number. 0. Scope Note. Proteins involved in ... Niemann-Pick C1 Protein [D12.776.543.585.594] * Nucleobase, Nucleoside, Nucleotide, and Nucleic Acid Transport Proteins [ ...
Protein * Band 3 anion transport protein / Homo sapiens P02730 # Export GlyConnect protein list related to protein with id 1024 ... Protein * Coat protein / Plum pox virus (strain r) # Export GlyConnect protein list related to protein with id 104 null # id : ... Protein * E3 SUMO-protein ligase RanBP2 / Homo sapiens P49792 # Export GlyConnect protein list related to protein with id 2890 ... Protein * Actin-binding LIM protein 1 / Homo sapiens O14639 # Export GlyConnect protein list related to protein with id 2904 ...
... of protein-rich grass silage was performed in experimental two-stage two-phase biogas reactor systems at low vs. increased ... Genes encoding different carbohydrate transport proteins were found among the most highly transcribed genes. MAG 18 also ... In particular, MAG 40 potentially is involved in decomposition of di- and monosaccharides trehalose, maltose and glucose. ... Further highly transcribed genes encode oligopeptide transport system proteins suggesting a hydrolytic lifestyle of this taxon. ...
  • D-Glucose/D-Galactose-binding protein (GGBP) mediates chemotaxis toward and active transport of glucose and galactose in a number of bacterial species. (nih.gov)
  • Carbohydrates and Their Absorption Simple sugars are monosaccharides (glucose, fructose and galactose) and disaccharides (sucrose, lactose, maltose) that are present in milk, fruits, and vegetables that add varying degrees of sweetness to food. (practicalgastro.com)
  • A sodium-dependent transporter moves the monosaccharides glucose and galactose into the enterocyte. (practicalgastro.com)
  • It spans the membrane of cells in these body systems and moves (transports) glucose and galactose from outside the cell to inside the cell. (medlineplus.gov)
  • In the intestinal tract, the SGLT1 protein helps the body absorb glucose and galactose from the diet so the body can use them. (medlineplus.gov)
  • however, other proteins in the kidneys are able to absorb enough glucose into the bloodstream, so that glucosuria is mild, if present at all, in people with glucose-galactose malabsorption. (medlineplus.gov)
  • O-glycosylation can be classified by the first monosaccharide attached to the protein: O-mannosylation (mannose), O-xylosylation (xylose), O-fucosylation (fucose), O-GalNAcylation (GalNAc), O-GlcNAcylation (GlcNAc), O-glucosylation (glucose) and O-galactosylation (galactose). (cdghub.com)
  • Also named "easy carbohydrates," these are molecules of very simple sugars such as glucose, fructose , and galactose, which are identified as monosaccharides. (totowin.xyz)
  • D-xylose ABC transporter, permease protein. (lbl.gov)
  • And just to make sure we're complete, I'm going to draw the protein transporter we have here as well as one on the other side, and show that there is a sodium ion that's flowing into our enterocyte down its concentration gradient to end up in the enterocyte with the sugar. (khanacademy.org)
  • 4] "The monosaccharide transporter gene family in land plants is ancient and shows differential subfamily expression and expansion across lineages. (tcdb.org)
  • Based on our findings, described below, we have now named YqgP protein as the glucose transporter protein or GluP. (biomedcentral.com)
  • O-linked protein glycosylation (or O-glycosylation) is the process by which glycans are added to specific amino acids, such as serine and threonine, in proteins. (cdghub.com)
  • During O-glycosylation, a monosaccharide is attached onto specific amino acids of a protein, either serine (S), threonine (T) and lysine (K). (cdghub.com)
  • During O-glycosylation, enzymes transfer monosaccharides from the activated sugars to amino acids (serine, threonine or lysine) on proteins, typically in the cytosol, Golgi, ER or nucleus. (cdghub.com)
  • In simple diffusion the movement of salute from the higher concentration of anything to the lower concentration through the membrane is produced or performed for example after digestion, a few monosaccharides diffuse into the bloodstream based on its concentration gradient a few examples would be glucose, amino acids and certain irons like chloride. (vedantu.com)
  • Through facilitated transport, some digested amino acids and glucose are absorbed into the blood or bloodstream with the help of this method. (vedantu.com)
  • SuSy proteins are usually homotetramers with an average monomeric molecular weight of about 90 kD (about 800 amino acids long). (frontiersin.org)
  • In non-photosynthetic tissues, the transported Suc is the raw material for many metabolic pathways, providing energy, as well as carbon skeletons for the production of organic matter such as amino acids, nucleotides and structural carbohydrates. (frontiersin.org)
  • Monosaccharides are sugars, sort of have a similar thing going on, but instead of primary active transport, we have what's called secondary active transport going on. (khanacademy.org)
  • Sodium and water are transported across the cell membrane along with the sugars in this process. (medlineplus.gov)
  • During the digestion of food, the protein transports the sugars into the cells that line the wall of the intestine (intestinal epithelial cells) as food passes through. (medlineplus.gov)
  • In addition, water that normally would have been transported with the sugars remains in the intestinal tract, resulting in dehydration of the body's tissues and severe diarrhea. (medlineplus.gov)
  • Protein glycosylation can be further divided into two main categories, N-linked and O-linked protein glycosylation , depending on how sugars are added to proteins. (cdghub.com)
  • O-glycosylation requires a variety of different enzymes to activate, modify and attach sugars to proteins and can be classified by the first sugar that is attached to the amino acid in the protein. (cdghub.com)
  • O-linked protein glycosylation (or O-glycosylation) is a process where sugars are attached to the oxygen atom of certain amino acid residues in proteins - mainly serine and threonine, as well as others, such as tyrosine and hydroxylysine 1-3 . (cdghub.com)
  • Before O-glycosylation can occur, cells must generate high-energy (reactive) forms of monosaccharides, called activated sugars 7 . (cdghub.com)
  • Consequently, the ER and Golgi contain membrane proteins called nucleotide sugar transporters that shuttle nucleotide sugars from the cytosol to the inside of the ER or Golgi 11,12 . (cdghub.com)
  • Enzymes may elongate the O-glycan chain in the Golgi by transferring additional monosaccharides from nucleotide sugars to the O-glycan chain. (cdghub.com)
  • 19, for motifs) by specific transporters signaling sites in IL12 molecules cause Ca2+-activated from KFT because the Direct gene of the transport course binds synaptic at oxidation. (erik-mill.de)
  • As the food travels down the gut, the smaller molecules are absorbed through the lining of the intestine and into the circulatory system (for carbohydrates and proteins) or into the lymphatic system (for fats). (easynotecards.com)
  • Nutrients and other molecules are imported into the cell, metabolized (broken down) and possibly synthesized into new molecules, modified if needed, transported around the cell, and possibly Simple carbs like table sugar are broken down quickly and are a fast source of energy, but eating them in excess will lead to fat storage. (fasttrackmerealestate.com)
  • A number of endogenous systems, such as the aerobic metabolism and electron transport chains, generate highly reactive molecules with important biological functions known as reactive oxygen species (ROS), including superoxide and hydrogen peroxide (H2O2). (bvsalud.org)
  • The cells in the small intestine have membranes that contain many transport proteins in order to get the monosaccharides and other nutrients into the blood where they can be distributed to the rest of the body. (bccampus.ca)
  • The proteins that we consume which are high in calorific value are broken down into simpler compounds by the action of pancreatic juice in the small intestine, the fats are similarly broken down by the action of bile which is also present in the small intestine and additionally small intestine helps in the absorption of food in various ways, the entire process would come under a simulation of food. (vedantu.com)
  • Once carbohydrates are chemically broken down into single sugar units they are then transported into the inside of intestinal cells. (bccampus.ca)
  • Crop biomass is rich in long-chained carbohydrates such as cellulose, hemicellulose, and xylan and additionally contains considerable amounts of proteins depending on the particular crop. (biomedcentral.com)
  • All carbohydrates must be broken down into monosaccharides for absorption. (practicalgastro.com)
  • These enzymes break down protein, fats, and carbohydrates. (easynotecards.com)
  • More coffee, tea, sodas, dry carbohydrates such as crackers, and protein bars all contribute to dehydration as the day goes on. (wholelifechallenge.com)
  • Lipids contain about 9 kcal/g (kilocalories per gram) while proteins and carbohydrates each have about 4 kcal/g. (usermessage.de)
  • macromolecule that makes up muscle, skin, organs, hair: protein: carbohydrates are made up of monomers called : monosaccharides: a disaccharide contains: two monosaccarides: polysaccharide that plants use to store energy: starch: polysaccharide animals use to store energy: glycogen: this organ stores glucose in the form of glycogen: liver: polysaccharide used … Which macromolecule stores energy, insulates us, and makes up the cell membrane? (usermessage.de)
  • With following highly we are - N-acetylneuraminic enzymes, proteins, alpha and surface, dyslexia, and Digestion - building autoreactivity suggests primarily we are. (erik-mill.de)
  • In the absence of enzymes that phosphorylate glucose, such as GlcK and/or enzyme II Glc , accumulated cytoplasmic glucose can be transported out of the cell. (biomedcentral.com)
  • The PTS comprises enzymes such as enzyme I (EI), enzyme II (EII), and histidine-containing phosphocarrier protein of the PTS (HPr). (biomedcentral.com)
  • Now the lining is present in the glands of the stomach to secrete hydrochloric acid that is responsible for dissolving food particles and protein-digesting enzymes called pepsin. (vedantu.com)
  • Water is also necessary to enable proteins that act as enzymes to function more efficiently. (wholelifechallenge.com)
  • Polysaccharides are long chains of monosaccharides. (practicalgastro.com)
  • The pectic cell wall fraction can be inexpensively and efficiently hydrolyzed into its component monosaccharides by enzymatic processes, as it is considerably less recalcitrant than other plant polysaccharides, such as cellulose. (biomedcentral.com)
  • How are muscle knobs have chylomicrons of cells of repair, attached over 10 peptides, with leaflets of membranes widely signaling, causing, and signaling invalid effect across Windows, OS X, well 10 cell monosaccharides? (erik-mill.de)
  • In an attempt to identify proteins present in these structures, GLUT4-enriched vesicles prepared from rat adipocytes treated with or without insulin were prepared by sucrose velocity gradient centrifugation and immunoadsorbed with anti-GLUT4 antibody. (umassmed.edu)
  • Acyl-CoA synthetase activity detected in GLUT4-enriched vesicles prepared by gradient centrifugation from insulin-treated adipocytes was decreased to about the same extent as GLUT4 protein. (umassmed.edu)
  • Anaerobic digestion (AD) of protein-rich grass silage was performed in experimental two-stage two-phase biogas reactor systems at low vs . increased organic loading rates (OLRs) under mesophilic (37 °C) and thermophilic (55 °C) temperatures. (biomedcentral.com)
  • Chloride is the major component of hydrochloric acid, which is needed for protein digestion. (wordpress.com)
  • Catabolism breaks down organic material to "produce" energy, and anabolism uses that energy to build critical cellular components like nucleic acids and proteins. (answerfitness.com)
  • Mutation in saposin D domain of sphingolipid activator protein gene causes urinary system defects and cerebellar Purkinje cell degeneration with accumulation of hydroxy fatty acid-containing ceramide in mouse, Human Molecular Genetics, Vol.13, No.21, 2709-2723, 2004. (tokushima-u.ac.jp)
  • They are a group of relatively rare inborn errors of metabolism caused by gene defects encoding proteins in the lysosomal degradation of sphingolipids [ 6 ]. (biomedcentral.com)
  • The SLC5A1 gene provides instructions for producing a protein called sodium/glucose cotransporter protein 1 (SGLT1). (medlineplus.gov)
  • SLC5A1 gene mutations impair or eliminate the function of the SGLT1 protein. (medlineplus.gov)
  • And so if we look at a single enterocyte or an intestinal cell, there would be a protein that's here on the cell membrane. (khanacademy.org)
  • And by allowing that to occur, energy is then harnessed allowing a monosaccharide or a sugar to enter into our enterocyte. (khanacademy.org)
  • The resultant monosaccharides are absorbed into the bloodstream and transported to the liver. (bccampus.ca)
  • The SGLT1 protein transports glucose into specialized kidney cells, ensuring that the sugar goes back into the bloodstream and is not released into the urine. (medlineplus.gov)
  • Which hormone is transported by the bloodstream? (medicalquiz.net)
  • Proteins involved in the transport of specific substances across the membranes of the MITOCHONDRIA . (nih.gov)
  • Transport substances across cell membranes. (answerfitness.com)
  • Additionally, immunoadsorbed GLUT4 vesicles were found to catalyze palmitoylation of proteins when incubated with labeled palmitate, a pathway that requires palmitate esterification with CoA. (umassmed.edu)
  • This system contains two homologues of the general secretory (Sec) pathway (SecA2 and SecY2) and several other essential proteins (Asp1 to Asp5) that share no homology to proteins of known function. (inrae.fr)
  • While triacylglycerol functions predominantly as an energy storage molecule, diacylglycerol and monoacylglycerol species perform signaling roles as secondary messengers or ligands for signaling proteins such as protein kinases. (news-medical.net)
  • Fructose is transported primarily by either GLUT-2 or GLUT-5 carrier proteins across the intestinal epithelium. (practicalgastro.com)
  • They circulate in the blood by binding to a protein carrier, serum albumin where they travel to the tissue for use in metabolism or biosynthetic pathways. (news-medical.net)
  • This can be defined as the process of movement of solute across the biological membrane present in the body with the help of certain specific carrier proteins. (vedantu.com)
  • Sucrose is a disaccharide that is the main transport sugar in plants and lactose is found only in mammalian milk. (totowin.xyz)
  • 5.) Absorption: is the passage of digested end products from the lumen of the GI tract through the mucosal cells by active or passive transport into the blood or lymph. (easynotecards.com)
  • Now we go on to define absorption so what happens during absorption is that the digested products are transported into the blood or lymph call lymphatic tissue is through the mucous membrane, the absorption of food is achieved by different mechanisms which are simple diffusion, active transport, facilitated transport and passive transport. (vedantu.com)
  • Consequently, a monosaccharide is transferred from a nucleotide sugar to dolichol phosphate on the cytosol side of the ER. (cdghub.com)
  • Plant SuSy isozymes are mainly located in the cytosol or adjacent to plasma membrane, but some SuSy proteins are found in the cell wall, vacuoles, and mitochondria. (frontiersin.org)
  • In plant photosynthesis, carbon dioxide is fixed in the chloroplasts via the Calvin cycle to yield triose phosphates (triose-P). Triose-P can be transported to the cytosol by a triose-P/phosphate translocator. (frontiersin.org)
  • approximately, the protein in acid side is enzymatic and, First to the central membrane of the Hamiltonian diseases, so following. (erik-mill.de)
  • This assists to make certain you get all the very important protein, fats, vitamins, minerals and fibre you need. (totowin.xyz)
  • Sucrose is the end product of photosynthesis and the primary sugar transported in the phloem of most plants. (frontiersin.org)
  • Sucrose-P is dephosphorylated by sucrose phosphate phosphatase (SPP) to form sucrose (Suc), the primary product of photosynthetic tissues and the main sugar transported from the source tissues through the phloem to non-photosynthetic tissues (sink tissues) in most plants ( Ruan, 2014 ). (frontiersin.org)
  • In contrast, the phosphorylation will not occur in non-PTS transported glucose. (biomedcentral.com)
  • Fructose is a monosaccharide, present in various foods such as sugar and honey, which needs to be metabolized by the liver to be transported into the blood in the form of protein. (tibhealth.com)
  • GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. (lbl.gov)
  • Water is needed to transport the nutrients required for maintenance and repair, whereby new cartilage is produced to replace the damaged cartilage cells. (wholelifechallenge.com)
  • A large group of membrane transport proteins that shuttle MONOSACCHARIDES across CELL MEMBRANES . (bvsalud.org)
  • These proteins are implicated in diverse pathways including cell proliferation, growth and protein transport. (news-medical.net)
  • O-glycans are needed for protein folding and stability, as well as biological processes, such as cell signalling, cell attachment, and cell recognition 7,8 . (cdghub.com)
  • Just like the term sounds, passive transport is a process where the movement of solute across a cell membrane takes place without the requirement of energy or expenditure of energy. (vedantu.com)
  • transport things through cell membrane. (usermessage.de)
  • A comparison with the closely related periplasmic receptors, ribose- and allose-binding proteins, shows that the GGBP hinge residue positions that undergo the largest conformational changes are different. (nih.gov)
  • While it has been reasoned that the optimization of cellular D-GalA uptake will be critical for the engineering of D-GalA utilization in yeast, no dedicated eukaryotic transport protein has been biochemically described. (biomedcentral.com)
  • We report here the sequence identification by high performance liquid chromatography-ion trap mass spectrometry of a p75 protein band, long chain acyl-CoA synthetase-1, specifically present in immunoadsorbed GLUT4-containing vesicles but not in vesicles adsorbed by nonimmune serum. (umassmed.edu)
  • The inactive form of the protein is localized in CYTOPLASMIC VESICLES. (ucdenver.edu)
  • It promotes transport of glucose from the BLOOD into target TISSUES. (ucdenver.edu)
  • Some terms (e.g.: blood) may appear in names of tissue, protein or disease or be included in an article title. (expasy.org)
  • The SGLT1 protein in kidney cells plays a role in maintaining normal blood glucose levels. (medlineplus.gov)
  • The process of electrolytes like any Na ions engages in the process of active transport as they are absorbed into the blood. (vedantu.com)
  • Xylose transport system permease protein XylH aka B3568, component of Xylose porter. (lbl.gov)