Bacteriocins: Substances elaborated by specific strains of bacteria that are lethal against other strains of the same or related species. They are protein or lipopolysaccharide-protein complexes used in taxonomy studies of bacteria.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Evolution, Molecular: The process of cumulative change at the level of DNA; RNA; and PROTEINS, over successive generations.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Bayes Theorem: A theorem in probability theory named for Thomas Bayes (1702-1761). In epidemiology, it is used to obtain the probability of disease in a group of people with some characteristic on the basis of the overall rate of that disease and of the likelihood of that characteristic in healthy and diseased individuals. The most familiar application is in clinical decision analysis where it is used for estimating the probability of a particular diagnosis given the appearance of some symptoms or test result.Pediococcus: A genus of gram-positive, facultatively anaerobic bacteria whose growth is dependent on the presence of a fermentable carbohydrate. No endospores are produced. Its organisms are found in fermenting plant products and are nonpathogenic to plants and animals, including humans.Lactobacillus: A genus of gram-positive, microaerophilic, rod-shaped bacteria occurring widely in nature. Its species are also part of the many normal flora of the mouth, intestinal tract, and vagina of many mammals, including humans. Pathogenicity from this genus is rare.Pyocins: Bacteriocins elaborated by mutant strains of Pseudomonas aeruginosa. They are protein or protein-lipopolysaccharide complexes lethal to other strains of the same or related species.Likelihood Functions: Functions constructed from a statistical model and a set of observed data which give the probability of that data for various values of the unknown model parameters. Those parameter values that maximize the probability are the maximum likelihood estimates of the parameters.Models, Genetic: Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.Fossils: Remains, impressions, or traces of animals or plants of past geological times which have been preserved in the earth's crust.Nisin: A 34-amino acid polypeptide antibiotic produced by Streptococcus lactis. It has been used as a food preservative in canned fruits and vegetables, and cheese.Software: Sequential operating programs and data which instruct the functioning of a digital computer.Listeria: A genus of bacteria which may be found in the feces of animals and man, on vegetation, and in silage. Its species are parasitic on cold-blooded and warm-blooded animals, including man.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Markov Chains: A stochastic process such that the conditional probability distribution for a state at any future instant, given the present state, is unaffected by any additional knowledge of the past history of the system.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Lactobacillaceae: A family of gram-positive bacteria found regularly in the mouth and intestinal tract of man and other animals, in food and dairy products, and in fermenting vegetable juices. A few species are highly pathogenic.RNA, Ribosomal, 18S: Constituent of the 40S subunit of eukaryotic ribosomes. 18S rRNA is involved in the initiation of polypeptide synthesis in eukaryotes.Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.Biological Evolution: The process of cumulative change over successive generations through which organisms acquire their distinguishing morphological and physiological characteristics.Gram-Positive Asporogenous Rods: A gram-positive, non-spore-forming group of bacteria comprising organisms that have morphological and physiological characteristics in common.Antibiosis: A natural association between organisms that is detrimental to at least one of them. This often refers to the production of chemicals by one microorganism that is harmful to another.Lactococcus: A genus of gram-positive, coccoid bacteria mainly isolated from milk and milk products. These bacteria are also found in plants and nonsterile frozen and dry foods. Previously thought to be a member of the genus STREPTOCOCCUS (group N), it is now recognized as a separate genus.Colicins: Bacteriocins elaborated by strains of Escherichia coli and related species. They are proteins or protein-lipopolysaccharide complexes lethal to other strains of the same species.Monte Carlo Method: In statistics, a technique for numerically approximating the solution of a mathematical problem by studying the distribution of some random variable, often generated by a computer. The name alludes to the randomness characteristic of the games of chance played at the gambling casinos in Monte Carlo. (From Random House Unabridged Dictionary, 2d ed, 1993)Gram-Positive Bacteria: Bacteria which retain the crystal violet stain when treated by Gram's method.Computational Biology: A field of biology concerned with the development of techniques for the collection and manipulation of biological data, and the use of such data to make biological discoveries or predictions. This field encompasses all computational methods and theories for solving biological problems including manipulation of models and datasets.Lactobacillus acidophilus: A species of gram-positive, rod-shaped bacteria isolated from the intestinal tract of humans and animals, the human mouth, and vagina. This organism produces the fermented product, acidophilus milk.Lactococcus lactis: A non-pathogenic species of LACTOCOCCUS found in DAIRY PRODUCTS and responsible for the souring of MILK and the production of LACTIC ACID.DNA, Mitochondrial: Double-stranded DNA of MITOCHONDRIA. In eukaryotes, the mitochondrial GENOME is circular and codes for ribosomal RNAs, transfer RNAs, and about 10 proteins.DNA, Ribosomal: DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.Leuconostoc: A genus of gram-positive, facultatively anaerobic bacteria whose growth is dependent on the presence of a fermentable carbohydrate. It is nonpathogenic to plants and animals, including humans.Enterococcus faecium: A species of gram-positive, coccoid bacteria whose organisms are normal flora of the intestinal tract. Unlike ENTEROCOCCUS FAECALIS, this species may produce an alpha-hemolytic reaction on blood agar and is unable to utilize pyruvic acid as an energy source.Listeria monocytogenes: A species of gram-positive, rod-shaped bacteria widely distributed in nature. It has been isolated from sewage, soil, silage, and from feces of healthy animals and man. Infection with this bacterium leads to encephalitis, meningitis, endocarditis, and abortion.Computer Simulation: Computer-based representation of physical systems and phenomena such as chemical processes.Genetic Variation: Genotypic differences observed among individuals in a population.Streptococcus mutans: A polysaccharide-producing species of STREPTOCOCCUS isolated from human dental plaque.Enterococcus faecalis: A species of gram-positive, coccoid bacteria commonly isolated from clinical specimens and the human intestinal tract. Most strains are nonhemolytic.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Hafnia alvei: The type species for the genus HAFNIA. It is distinguished from other biochemically similar bacteria by its lack of acid production on media containing sucrose. (From Bergey's Manual of Determinative Bacteriology, 9th ed)Microbial Sensitivity Tests: Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).Streptococcus: A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Yogurt: A slightly acid milk food produced by fermentation due to the combined action of Lactobacillus acidophilus and Streptococcus thermophilus.Clostridium perfringens: The most common etiologic agent of GAS GANGRENE. It is differentiable into several distinct types based on the distribution of twelve different toxins.Enterococcus: A genus of gram-positive, coccoid bacteria consisting of organisms causing variable hemolysis that are normal flora of the intestinal tract. Previously thought to be a member of the genus STREPTOCOCCUS, it is now recognized as a separate genus.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Genes, Bacterial: The functional hereditary units of BACTERIA.Bacterial Proteins: Proteins found in any species of bacterium.Food Preservation: Procedures or techniques used to keep food from spoiling.Mitomycins: A group of methylazirinopyrroloindolediones obtained from certain Streptomyces strains. They are very toxic antibiotics used as ANTINEOPLASTIC AGENTS in some solid tumors. PORFIROMYCIN and MITOMYCIN are the most useful members of the group.Bacteriophages: Viruses whose hosts are bacterial cells.Spheroplasts: Cells, usually bacteria or yeast, which have partially lost their cell wall, lost their characteristic shape and become round.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Bacillus: A genus of BACILLACEAE that are spore-forming, rod-shaped cells. Most species are saprophytic soil forms with only a few species being pathogenic.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
... s are bacteriocins produced by halophilic Archaea and a type of archaeocin. Since their discovery in 1982, halocins have ... Cheung J, Danna KJ, O'Connor EM, Price LB, Shand RF (1997). "Isolation, sequence, and expression of the gene encoding halocin ... Molecular Microbiology. 57 (2): 537-49. doi:10.1111/j.1365-2958.2005.04705.x. PMID 15978083. Perez, AM (2000). Growth ... Like other bacteriocins, the halocins are under investigation as antimicrobials for use in controlling spoilage during ...
These enzymes became known as restriction enzymes and developed into one of the main molecular tools in molecular biology. In ... he found that bacteriocins impair the function of cell membranes. Returning to MIT, his lab discovered that bacteriocins ... It was later discovered by other researchers that bacteria produce enzymes that cut viral DNA at particular sequences but not ... Roberts RJ (April 2005). "How restriction enzymes became the workhorses of molecular biology". Proc. Natl. Acad. Sci. U.S.A. ...
Comprehensive sequence searches across multiple taxonomic groups have failed to identify MHC and TCRs outside of the jawed ... For other articles which focus on its importance in medicine, molecular biology, and so forth, the following topics are ... Bacteria, for instance, secrete bacteriocins, proteinaceous toxins specifically targeted against members of their own species. ... Interestingly, sequence variability in "hot spots" have been identified in these receptors. It would appear that molecules ...
"Analysis of the Genome Sequence of Lactobacillus gasseri ATCC 33323 Reveals the Molecular Basis of an Autochthonous Intestinal ... 1]Pandey N, Malik RK, Kaushik JK, Singroha G (2013). "Gassericin A: a circular bacteriocin produced by lactic acid bacteria ... Its genome has been sequenced. L. gasseri is a normal inhabitant of the lower reproductive tract in healthy women. It also ... L. gasseri produces gassericin A, a bacteriocin. Lauer, Eckhard, and Otto Kandler. " Lactobacillus gasseri sp. nov., a new ...
February 2011). "Genome Sequence of Weissella cibaria KACC 11862". Journal of Bacteriology. 193 (3): 797-8. doi:10.1128/JB. ... Srionnual, S.; Yanagida, F.; Lin, L.-H.; Hsiao, K.-N.; Chen, Y.-s. (2007). "Weissellicin 110, a Newly Discovered Bacteriocin ... W.cibaria CMGDEX3 was reported from Pakistan to produce high molecular weight,linear dextran with predominant (1→6) linkages. ... 2012). "Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3". Carbohydrate Polymers. 90: ...
Most of the molecular mechanisms are not known, however. L. rhamnosus GG is beneficial in the prevention of rotavirus diarrhea ... The genome sequence of Lactobacillus rhamnosus GG (ATCC 53103) has been decoded. In 1983, Lactobacillus rhamnosus GG was ... bacteriocin production, pili production, the cassystem, the clustered regularly interspaced short palindromic repeat (CRISPR) ... Morita H, Toh H, Oshima K, Murakami M, Taylor TD, Igimi S, Hattori M (December 2009). "Complete genome sequence of the ...
Bacteriocin genes are located either on chromosoms or on plasmids. Strain 5 produces a plasmid-encoded bacteriocin that is ... Mørtvedt, C. I.; Nissen-Meyer, J.; Sletten, K.; Nes, I. F. (1991). "Purification and amino acid sequence of lactocin S, a ... 4, pages 1359-1365 (abstract) Schmidt, G.; Hertel, C.; Hammes, W. P. (1999). "Molecular Characterisation of the dnaK Operon of ... Sakacins are bacteriocins of class II produced by L. sakei. In strain CCUG 42687, their production is dependent on nutrients, ...
The porins act as molecular filters for hydrophilic compounds. They are responsible for the 'molecular sieve' properties of the ... Structural and sequence analysis provide firm evidence that the GBP, SP and RPP families together with 44 additional families ... they also serve as receptor sites for the binding of phages and bacteriocins. General diffusion porins usually assemble as a ... Pattus F, Jeanteur D, Lakey JH (1991). "The bacterial porin superfamily: sequence alignment and structure prediction". Mol. ...
The molecular mass of the mature HalH4 protein is 34.9 kDa (359 amino acids), processed from a preprotein of 39.6 kDa; the ... Cheung J; Danna KJ; O'Connor EM; Price LB; Shand RF (1997). "Isolation, sequence, and expression of the gene encoding halocin ... H4, a bacteriocin from the halophilic archaeon Haloferax mediterranei R4". J. Bacteriol. 179 (2): 548-51. PMC 178729 . PMID ... These antimicrobial peptides range from ~3 to 7.4 kDa in molecular mass, consisting of 36 to 76 amino acid residues. Two of the ...
High-throughput sequencing and the whole genome sequencing approaches will provide the further information about the complexity ... Human microbiome List of human flora Bacteriocin Erb-Downward, John R.; Thompson, Deborah L.; Han, Meilan K.; Freeman, ... journal of molecular epidemiology and evolutionary genetics in infectious diseases. 21: 563-74. doi:10.1016/j.meegid.2013.04. ... The DNA-binding protein complex recognizes a discrete nucleotide sequence (5'-GGG ACT TTC T-3') in the upstream region of a ...
Some of the Corynebacterium species with sequenced genomes have between 2.5 and 3.0 million base pairs. The bacteria grow in ... Kerry-Williams, S.M.; Noble, W.C. (1984). "Plasmid-associated bacteriocin production in a JK-type coryneform bacterium". FEMS ... Gao, B.; Gupta, R. S. (2012). "Phylogenetic Framework and Molecular Signatures for the Main Clades of the Phylum Actinobacteria ... In phylogenetic trees based on concatenated protein sequences or 16S rRNA, the genus Corynebacterium forms a distinct clade, ...
sequenced the genome of IL1403, which coincided with a significant shift of resources to understanding LAB genomics and related ... Proteinaceous bacteriocins are produced by several LAB strains and provide an additional hurdle for spoilage and pathogenic ... However, there is a wide variation in molecular structures of exopolysaccharides and the complexity of the mechanisms by which ... Mc Grath S and van Sinderen D (editors). (2007). Bacteriophage: Genetics and Molecular Biology (1st ed.). Caister Academic ...
Provides information on molecular mass, composition, sequence, and sometimes branching of a glycan chain. It can also be used ... a new post-translational modification found in glycopeptide bacteriocins". FEBS Letters. 585 (4): 645-650. doi:10.1016/j. ... To identify specific sugars, their sequence, linkages, and the anomeric nature of glycosidic chain. ... provides information on molecular mass, protein-carbohydrate ratio, aggregation state, size, and sometimes branching of a ...
... and tRNA-Ala genes at 403 base pairs for the complete sequence. Other genes that have been sequenced include the following: ... Gilberts, EC; Greenstein, AJ; Katsel, P; Harpaz, N; Greenstein, RJ (1994). "Molecular evidence for two forms of Crohn disease ... Finally, they produce antimicrobial compounds, such as organic acids (lactate, acetate, and others), bacteriocins, and ... L. Pontis was classified as a new species based on the 16S rRNA gene sequence. Strain LTH 2587T, also known as DSM 8475/ LMG ...
It shows significant sequence similarity to human ABC transporters of subfamily B that includes MDR1 and TAP1/TAP2. The ATPase ... The precise molecular mechanism of ATP hydrolysis is still controversial. ABC transporters are active transporters, that is, ... One class is involved in the protein (e.g. toxins, hydrolytic enzymes, S-layer proteins, lantibiotics, bacteriocins, and ... The sequence and architecture of TMDs is variable, reflecting the chemical diversity of substrates that can be translocated. ...
Research teams have sequenced the genome of two strains of S. thermophilus, CNRZ1066 and LMG13811, and stated that the bacteria ... Kiliç, AO; Pavlova, SI; Ma, WG; Tao, L (1996). "Analysis of Lactobacillus phages and bacteriocins in American dairy products ... "Mixed-Culture Transcriptome Analysis Reveals the Molecular Basis of Mixed-Culture Growth in Streptococcus thermophilus and ... At least 26 strains of S. thermophilis have been identified and had their genomes sequenced. S. thermophilus is one of the most ...
The genome is composed of ~15-kb linear double-stranded DNA (dsDNA) with long inverted terminal repeat sequences (100 bp). ... Naclerio, G; Ricca, E; Sacco, M; De Felice, M (December 1993). "Antimicrobial activity of a newly identified bacteriocin of ... Molecular Microbiology. 59 (5): 1602-9. doi:10.1111/j.1365-2958.2006.05044.x. PMC 1413580 . PMID 16468998. Vilà, B.; Fontgibell ... Some strains of B. cereus produce cereins, bacteriocins active against different B. cereus strains or other Gram-positive ...
... colicin-like bacteriocins are 20 to 90 kDa in size and tailocins or so called high molecular weight bacteriocins which are ... Mørtvedt, C. I.; Nissen-Meyer, J.; Sletten, K.; Nes, I. F. (1991). "Purification and amino acid sequence of lactocin S, a ... They are the longest studied bacteriocins. They are a diverse group of bacteriocins and do not include all the bacteriocins ... The class I bacteriocins are small peptide inhibitors and include nisin and other lantibiotics. The class II bacteriocins are ...
The lantibiotics are a class of more extensively modified bacteriocins, also called Class I bacteriocins. (Bacteriocins for ... They are synthesised with a leader polypeptide sequence that is removed only during the transport of the molecule out of the ... "New insights into the mechanism of action of lantibiotics-diverse biological effects by binding to the same molecular target". ... they are considered a member of the bacteriocins. Bacteriocins are classified according to their extent of posttranslational ...
... were fully sequenced. A. tumefaciens C58, the first fully sequenced pathovar, was first isolated from a cherry tree crown gall ... This biosynthetic pathway is not used in many plants for the production of auxin, so it means the plant has no molecular means ... K84 produces a bacteriocin (agrocin 84) which is an antibiotic specific against related bacteria, including A. tumefaciens.This ... This is done by cloning a desired gene sequence into the T-DNA that will be inserted into the host DNA. This process has been ...
Researchers also often upload plasmid sequences to the NCBI database, from which sequences of specific plasmids can be ... "Microbiology and Molecular Biology Reviews. 74 (3): 434-52. doi:10.1128/MMBR.00020-10. PMC 2937521. PMID 20805406.. ... Col plasmids, which contain genes that code for bacteriocins, proteins that can kill other bacteria. ... "Current Opinion in Molecular Therapeutics. 11 (4): 433-41. PMID 19649988. Archived from the original on 17 September 2011.. ...
2009). Lactobacillus Molecular Biology: From Genomics to Probiotics. Caister Academic Press. ISBN 978-1904455417. [page needed ... Recent developments of high-throughput sequencing technology and the consequent progresses of metagenomics represent a new ... and characterization of two bacteriocins produced by isolates of Enterococcus faecium". Journal of Applied Microbiology. The ... Research into the potential health effects of supplemental probiotics has included the molecular biology and genomics of ...
A method for promoting plant growth and/or disease resistance comprising applying a purified polypeptide that is a bacteriocin ... The molecular weight of the ion for sequencing was slightly less than the initially determined molecular weight of 3162 Da ( ... Many but not all bacteriocins are of low-molecular weight, in the range of about 100 to about 10,000 Daltons. Bacteriocins are ... Furthermore, the sequence information for Plantaricin S, a bacteriocin produced from Lactobacillus plantarum LPCO10 requiring ...
MALDI-TOF experiments determined the precise molecular mass of the peptide to be of 5.6 kDa and N-terminal sequencing of the ... Sequence analysis of the putative bacteriocin gene did not show significant similarity to any reported bacteriocin producing ... by 16 S rRNA gene sequence analysis. The bacteriocin produced by strain GI-9, namely, laterosporulin was purified from ... The bacteriocin was active against a wide range of Gram-positive and Gram-negative bacteria. ...
... bacH encoded a 195-residue sequence with a molecular mass of 21.9 kDa. The deduced amino acid sequence showed significant ... bacA encoded a 105-residue sequence with a molecular mass of 11.1 kDa. The deduced BacA protein showed 100% homology to the ... The smallest fragment (4.5 kb) retaining some degree of bacteriocin expression contained the bacA to bacE sequences located in ... Cloning and genetic and sequence analyses of the bacteriocin 21 determinant encoded on the Enterococcus faecalis pheromone- ...
The nisin Q gene cluster was sequenced, and 11 putative orfs having 82% homology with the nisin A biosynthesis gene cluster ... Molecular Sequence Data. Multigene Family / genetics. Nisin / analogs & derivatives*, biosynthesis, genetics, pharmacology. ... Bacteriocins / genetics*, metabolism, pharmacology. DNA, Bacterial / genetics. Lactococcus lactis / genetics*, metabolism. ... The nisin Q gene cluster was sequenced, and 11 putative orfs having 82% homology with the nisin A biosynthesis gene cluster ...
... www.uniprot.org/help/sequences">molecular weight,/a>. The information is filed in different subsections. The current ... IPR009086, Bacteriocin_AS48. ,p>This section displays by default the canonical protein sequence and upon request all isoforms ... Sequence databases. Select the link destinations:. EMBL nucleotide sequence database. More...EMBLi. GenBank nucleotide sequence ... p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates ...
Non-coding RNAs in Gram postive bacteria; bacteriocins. Dr Daniel Mitchell Molecular basis for immune system interactions with ... Bacteriology; antibiotic resistance; mobile genetic elements; transcriptomics; next-generation sequencing; Haemophilus ... Bioinformatics and laboratory-based molecular bacteriology.. Professor Matthew Gibson Organic, polymer and carbohydrate ...
Amino Acid Sequence, Bacteriocins, Base Sequence, DNA, Bacterial, Enterococcus faecium, Molecular Sequence Data, Open Reading ... Complete sequence of the enterocin Q-encoding plasmid pCIZ2 from the multiple bacteriocin producer Enterococcus faecium L50 and ... Complete sequence of the enterocin Q-encoding plasmid pCIZ2 from the multiple bacteriocin producer Enterococcus faecium L50 and ... in the multiple bacteriocin producer Enterococcus faecium strain L50 were determined. These bacteriocin genes occur at ...
Research Topics about Experts and Doctors on molecular sequence data in Edmonton, Alberta, Canada ... Subtilosin A (1) represents a new class of bacteriocins. ... *. Lu J, Edwards R, Wong J, Manchak J, Scott P, Frost L, et al. ... Experts and Doctors on molecular sequence data in Edmonton, Alberta, Canada. Summary. Locale: Edmonton, Alberta, Canada ... You are here: Locale , Canada , Alberta , Experts and Doctors on molecular sequence data in Edmonton, Alberta, Canada ...
Lactacin B is a class II bacteriocin produced by L. acidophilus NCFM (24). The genome sequence of L. acidophilus NCFM ... PNAS Profile of NAS member and molecular biologist Rodolphe Barrangou. Image courtesy of North Carolina State University/Marc ... Sequence repeat lengths, in bp, are displayed by color coding as indicated by the colored boxes. The sequence 5′- ... M.C. and S.L. contributed to manual annotation; A.H. and R.C. provided DNA sequence and assembly data. Contract sequencing for ...
Amino Acid Sequence · Bacteriocins · Base Sequence · Cloning, Molecular · Codon, Initiator · Codon, Terminator · DNA, Bacterial ... DNA sequence · genetic code · Lactobacillus gasseri · molecular cloning · nonhuman · nucleotide sequence · open reading frame ... Standard procedures for molecular techniques were used to locate, clone and sequence the fragments of LF221 chromosomal DNA ... Molecular weight · Mouth disease · Nonhuman · Obesity · Pyrosequencing · Respiratory tract infection · Review · RNA sequence · ...
These enzymes became known as restriction enzymes and developed into one of the main molecular tools in molecular biology. In ... he found that bacteriocins impair the function of cell membranes. Returning to MIT, his lab discovered that bacteriocins ... It was later discovered by other researchers that bacteria produce enzymes that cut viral DNA at particular sequences but not ... Roberts RJ (April 2005). "How restriction enzymes became the workhorses of molecular biology". Proc. Natl. Acad. Sci. U.S.A. ...
The N-terminal amino acid sequence of dysgalacticin [Asn-Glu-Thr-Asn-Asn-Phe-Ala-Glu-Thr-Gln-Lys-Glu-Ile-Thr-Thr-Asn-(Asn)-Glu- ... Unlike many previously described bacteriocins of Gram-positive bacteria, dysgalacticin is a heat-labile 21.5 kDa anionic ... Natural dysA variants containing conservative amino acid substitutions were also detected by sequence analyses of dysA elements ... Moreover, dysgalacticin displays similarity in predicted secondary structure (but not primary amino acid sequence or inhibitory ...
Halocins are bacteriocins produced by halophilic Archaea and a type of archaeocin. Since their discovery in 1982, halocins have ... Cheung J, Danna KJ, OConnor EM, Price LB, Shand RF (1997). "Isolation, sequence, and expression of the gene encoding halocin ... Molecular Microbiology. 57 (2): 537-49. doi:10.1111/j.1365-2958.2005.04705.x. PMID 15978083. Perez, AM (2000). Growth ... Like other bacteriocins, the halocins are under investigation as antimicrobials for use in controlling spoilage during ...
MOLECULAR BIOLOGY OF PATHOGENS. Role of Bacteriocin Immunity Proteins in the Antimicrobial Sensitivity of Streptococcus mutans ... an analysis revealed that the replacement of the comC gene with an Ermr gene also removed a portion of the nucleotide sequence ... mutans GS5 is modulated by some of the putative bacteriocin immunity proteins expressed by the organism. Since the bacteriocin ... A role for bacteriocin immunity proteins in resistance to some noncognate bacteriocins was suggested by the demonstration that ...
We compared a set of 42 ST40 isolates by assessing key molecular determinants, performing whole genome sequencing (WGS) and a ... Molecular, genomic and phenotypic analysis of representative isolates of a major clone of E. faecalis MLST ST40 revealed new ... and animal experiments to compare the isolate D32 with a closely related ST40 endocarditis isolate whose draft genome sequence ... because of their production of secondary metabolites and bacteriocins . On the other hand, enterococci, in particular E. ...
In silico analyses suggested that endophycin L is a toxin-like bacteriocin, this bacteriocin could be a potential candidate as ... A endoficina L parcialmente purificada apresentou um peso molecular estimado de 14 kDa e atividade in gel, e atingiu seu nível ... flaccumfaciens was selected for its bacteriocinogenic ability The fragment was subcloned and DNA sequencing revealed a 2-kb ... Bacteriocins are antimicrobial proteins produced by bacteria, which play a role in microbial ecology. In this work we examined ...
Structure (Molecular Modeling Database). *Vector Alignment Search Tool (VAST). *All Domains & Structures Resources... ... Cloning and sequencing of curA encoding curvacin A, the bacteriocin produced by Lactobacillus curvatus LTH1174.. Tichaczek PS1 ... CurA was cloned into Escherichia coli NM554 and its nucleotide sequence was determined. Sequencing revealed the presence of an ... Curvacin A is synthesised as a prepeptide of 59 amino acids which is proteolytically processed to the mature bacteriocin of 41 ...
Mass Spectrometry and Amino Acid Sequence. The molecular mass of the purified pentocin JL-1 was detected by MALDI-TOF-MS ( ... Many bacteriocins such as bacteriocin KU24 , bacteriocin VJ13 , plantaricin ZJ5 , and other LAB bacteriocins share ... Purification of the Bacteriocin. The bacteriocin, pentocin JL-1, is one of the secondary metabolites of L. pentosus JL-1 after ... The 16S rRNA gene sequence was amplified by PCR, and a 1485 bp gene fragment was sequenced after cloning and aligned using the ...
The molecular mass of the purified protein is 24 kDa (Fig. 4a), and the N-terminal sequence, DDVKN, exactly matched the ... Sequence and analysis of the 60 kb conjugative, bacteriocin-producing plasmid pMRC01 from Lactococcus lactis DPC3147.Mol. ... Collected sequences were assembled by using the SEQUENCHER DNA sequencing software (v.3.0; Gene Codes). The sequences of both ... Molecular Pathogenesis. Complete Nucleotide Sequence of aStaphylococcus aureus Exfoliative Toxin B Plasmid and Identification ...
were isolated and subsequently identified based on biochemical and molecular methods. According to the sequencing of isolates, ... The specific primers of bacteriocin used in this study are shown in Table 1. According to the bacteriocins sequences of ... and M12 had bre174A bacteriocin gene (Fig. 4; Table 7). Sequence and culture deposition Sequences were submitted to the NCBI ... were isolated and subsequently identified based on biochemical and molecular methods. According to the sequencing of isolates, ...
Molecular Sequence Data * Peptides* * Peptides, Cyclic / isolation & purification * Peptides, Cyclic / pharmacology * ... Bacteriocins * Chromatography, High Pressure Liquid * Chromatography, Ion Exchange * Gas Chromatography-Mass Spectrometry ... of the polypeptide containing four thioether bridges involved mainly a combination of automated gas-phase sequencing, ...
Molecular Sequence Data. *Pheromones/biosynthesis. *Pheromones/chemistry*. *Pheromones/physiology. *Protein Structure, ... Production of bacteriocins by lactic acid bacteria is in some cases regulated by a quorum sensing mechanism that involves a ... PlnA-22L induced bacteriocin production in L. plantarum C11 (i.e., displayed pheromone activity), the level of induction being ... To gain insight into how PlnA functions as a pheromone and as a bacteriocin, the L- and D-enantiomers of an N-terminally ...
GccF is a rare example of a glycoactive bacteriocin, with both the O-linked N-acetylglucosamine (GlcNAc) and the unusual S- ... cMaurice Wilkins Centre for Molecular Biodiscovery, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand d ... Strikingly, the bioactivity increased significantly when the sequence incorporated two S-GlcNAc moieties at positions 18 and 43 ... Glycocin F (GccF, 1) is a potent bacteriocin originally isolated from liquid culture of Lactobacillus plantarum KW30.6 GccF is ...
... bacteriocins; molecular weight; nucleotide sequences; polyacrylamide gel electrophoresis; polymerase chain reaction; rumen; ... Bacteriocin produced by E. faecalis II/1 was purified to homogeneity. Purified bacteriocin exhibited a single band on sodium ... Purification and partial characterization of an extracellular bacteriocin produced by the ruminal isolate Enterococcus faecalis ... DNA; Enterococcus faecalis; Gram-positive bacteria; amino acid sequences; amino acids; antibacterial proteins; ...
CharacterizationBacteriaAntimicrobial peptidesPeptidesEnterococcusPlasmidBacterialProbioticAmino acidsSpeciesImmunityLantibioticsHomologyIsolatePATHOGENSProduces a novel bacteriocinLactococcusNucleotide sequencePhylogeneticStaphylococcusAntibioticsPurificationHydrogen peroxideNisinEnterocinLactisPost-translationallyClostridiumPlasmidsProductionBiochemical and molecular methodsClustersProbiotics
- Identification, Purification and Characterization of Laterosporulin, a Novel Bacteriocin Produced by Brevibacillus sp. (plos.org)
- Complete sequence of the enterocin Q-encoding plasmid pCIZ2 from the multiple bacteriocin producer Enterococcus faecium L50 and genetic characterization of enterocin Q production and immunity. (surrey.ac.uk)
- Purification and partial characterization of an extracellular bacteriocin produced by the ruminal isolate Enterococcus faecalis II/1 and determine the frequency of occurrence of enterolysin A structural gene within the ruminal cocci. (usda.gov)
- Griffon vulture feces were screened for LAB with antimicrobial activity, genes encoding bacteriocins, potential virulence determinants, susceptibility to antibiotics, genotyping and characterization of bacteriocins. (biomedcentral.com)
- A multiple-step chromatographic procedure, determination of the N-terminal amino acid sequence of purified bacteriocins by Edman degradation and a MALDI TOF/TOF tandem MS procedure permitted characterization of bacteriocins present in supernatants of producer cells. (biomedcentral.com)
- BACTIBASE is an integrated open-access database designed for the characterization of bacterial antimicrobial peptides, commonly known as bacteriocins. (biomedcentral.com)
- We have also integrated several applications and utilities for bacteriocin sequences analysis and characterization. (biomedcentral.com)
- The authors explore culture methods and species selective growth media, to grow, separate, and characterize LAB and dairy associated species, molecular methods for species identification and strains characterization, Next Generation Sequencing for genome characterization, comparative genomics, phenotyping, and current applications in dairy and non-dairy productions. (wiley.com)
- Purification and characterization of bacteriocin from Klebsiella pneumoniae 158. (microbiologyresearch.org)
- Structural analysis and characterization of lacticin Q, a novel bacteriocin belonging to a new family of unmodified bacteriocins of gram-positive bacteria. (mysciencework.com)
- Different functions have already been found on these plasmids, among which are genes for lactose metabolism, bacteriocin synthesis, exopolysaccharide production, or DNA restriction-modification (R-M). With the development of molecular biology and functional genomics of lactobacilli, interest is growing for the characterization of replicons themselves as potential useful (food-grade) vectors. (asm.org)
- Purification and characterization of thermophilin T, a novel bacteriocin produced by Streptococcus thermophilus ACA-DC 0040. (microbiologyresearch.org)
- Upon purification and subsequent molecular characterization of the antimicrobial peptide, referred to as penisin, we found the peptide to be a bacteriocin-like peptide. (osdd.net)
- Partial Characterization of Bacteriocin Produced by Halotolerant Pediococcus acidilactici Strain QC38 Isolated from Traditional Cotija Cheese. (pjmonline.org)
- Isolation and characterization of a highly hydrophobic new bacteriocin (gassericin A) from Lactobacillus gasseri LA39. (edu.au)
- 1. Conduct a functional and molecular characterization of Shiga-toxin producing Escherichia coli (STEC) with specific emphasis elucidating the responses to food-related stresses, and genomic and proteomic studies to assess changes in virulence and pathogenicity. (usda.gov)
- 2: Conduct functional and molecular characterization of Campylobacter species with specific emphasis on responses to intrinsic and extrinsic stresses through genomic and proteomic studies, and examination of morphological and physiological changes. (usda.gov)
- It was later discovered by other researchers that bacteria produce enzymes that cut viral DNA at particular sequences but not the bacteria's own DNA, which is protected by methylation. (wikipedia.org)
- Unlike many previously described bacteriocins of Gram-positive bacteria, dysgalacticin is a heat-labile 21.5 kDa anionic protein that kills its target without inducing lysis. (microbiologyresearch.org)
- Many gram-positive bacteria produce antimicrobial peptides called bacteriocins ( 4 , 8 , 20 , 24 ). (asm.org)
- Bacteriocins are antimicrobial proteins produced by bacteria, which play a role in microbial ecology. (usp.br)
- Particularly, the bacteriocins produced by lactic acid bacteria (LAB) have been the focus of much research because LAB and their metabolic products are generally regarded as safe (GRAS) [ 12 ] and have potential application as natural preservatives in the food industry [ 13 ]. (hindawi.com)
- Lactobacilli, as the largest group of lactic acid bacteria, produce large amounts of antimicrobial metabolites such as organic acids, fatty acids, ammonia, hydrogen peroxide, diacetyl and bacteriocin, which inhibit the growth of pathogenic bacteria and increase shelf life of food. (paperity.org)
- Production of bacteriocins by lactic acid bacteria is in some cases regulated by a quorum sensing mechanism that involves a secreted bacteriocin-like peptide pheromone. (nih.gov)
- Glycocin F (GccF) is a unique diglycosylated bacteriocin peptide that possesses potent and reversible bacteriostatic activity against a range of Gram-positive bacteria. (rsc.org)
- 1 Bacteriocins are ribosomally synthesized and post-translationally modified peptides (RiPPs) secreted by bacteria as a primary defence mechanism against competing bacteria. (rsc.org)
- 2 Bacteriocins from lactic acid bacteria have received considerable attention due to a desirable safety profile, stability across wide pH and temperature ranges, antimicrobial activity against various pathogens, and no cross-resistance with antibiotics. (rsc.org)
- In this review, current knowledge of how the extraordinary range of bacteriocin diversity arose and is maintained in one species of bacteria, Escherichia coli , is assessed and the role these toxins play in mediating microbial dynamics is discussed. (asmscience.org)
- 1976. Bacteriocins of gram-positive bacteria. (asmscience.org)
- 1988. Bacteriocins of lactic acid bacteria. (asmscience.org)
- The purpose of this study was to isolate and characterize bacteriocin-producing bacteria against Clostridium perfringens from domestic animals to determine their usefulness as probiotics. (koreascience.or.kr)
- The feces of cattle and chicken were used as sources to isolate bacteriocin-producing bacteria using the spot-on-lawn method. (koreascience.or.kr)
- The isolated bacteriocin-producing bacteria and/or bacteriocin have the potential to be used as probiotics in the livestock industry. (koreascience.or.kr)
- Hechard, Y. and Sahl, H. G. (2002) Mode of action of modified and unmodified bacteriocins from Gram-positive bacteria. (koreascience.or.kr)
- Lactic acid bacteria (LAB) are part of the gut microbiota and produce ribosomally synthesized antimicrobial peptides or bacteriocins with interest as natural food preservatives and therapeutic agents. (biomedcentral.com)
- Therefore, griffon vultures might be considered a reservoir of bacteriocin-producing lactic acid bacteria (LAB) with potential biotechnological applications. (biomedcentral.com)
- Bacteriocins are non-toxic inhibitors of bacteria and thus represent potential alternatives or complements to conventional antibiotics in the treatment of infections and in livestock production. (biomedcentral.com)
- The fifth edition of this highly successful book provides students with an essential introduction to the molecular genetics of bacteria covering the basic concepts and the latest developments. (nhbs.com)
- We report nine genome sequences representing the phylogenetic and functional diversity of these bacteria. (pnas.org)
- Class IIa bacteriocins from lactic acid bacteria are small, cationic proteins with antilisterial activity. (biomedcentral.com)
- The bacteriocins produced by Gram-negative bacteria are most often large proteins (many are larger that 20 kDa) and their inhibition spectrum is rather narrow, spanning to closely related species. (biomedcentral.com)
- Gram-positive bacteria most often produce peptide bacteriocins smaller than 6 kDa. (biomedcentral.com)
- The bacteriocins produced by lactic acid bacteria (LAB) are divided into three main groups: the lantibiotics, which are modified bacteriocins (class I), the nonlantibiotics, which are heat-stable and unmodified (class II), and a group of large heat-labile bacteriocins (class III). (biomedcentral.com)
- Lactic acid bacteria and bacteriocins: their practical importance. (microbiologyresearch.org)
- Lactic acid bacteria (LAB) from cow and goat milk have been well studied for both antimicrobial activity and bacteriocin production [4- (cyberleninka.org)
- Molecular identification of bacteria associated with bacterial vaginosis. (semanticscholar.org)
- In contrast to most bacteriocins produced by gram-positive bacteria, lacticin Q had no N-terminal extensions such as leader or signal sequences. (mysciencework.com)
- diacetylactis BGBU1-4 produces a novel bacteriocin, lactolisterin BU, with strong antimicrobial activity against many species of Gram-positive bacteria, including important food spoilage and foodborne pathogens, such as Listeria monocytogenes, Staphylococcus aureus, Bacillus spp. (ac.rs)
- Lactolisterin BU showed the highest similarity to aureocin-like bacteriocins produced by different bacteria. (ac.rs)
- Non- aureus staphylococci (NAS), the bacteria most commonly isolated from the bovine udder, potentially protect the udder against infection by major mastitis pathogens due to bacteriocin production. (asm.org)
- Bacteriocins are ribosomally synthesized and posttranslationally modified peptides (RiPPs) produced by Gram-positive bacteria that mainly inhibit growth of similar bacterial species or occasionally a broad spectrum of bacteria ( 9 ). (asm.org)
- These types of microcins are low molecular weight antibiotic peptides produced by Enterobacteriaceae with a wide range of antibacterial activity against Gram-negative bacteria. (kowsarpub.com)
- Bacteriocins are synthesized by ribosomes in bacteria that are active against closely related strains. (kowsarpub.com)
- Bacteriocins are ribosomally synthesized antimicrobial peptides produced by bacteria that deserve considerable interest for their use as natural and nontoxic food preservatives as well as for their potential use in human and veterinary applications, and in the animal production field [ 1 ]. (hindawi.com)
- defined bacteriocins as proteinaceous compounds that kill closely related bacteria but it is now recognized that bacteriocins can also have activity across genera (broad spectrum) [ 2 - 4 ]. (hindawi.com)
- Amino acid replacements in previously isolated inactive colicin M mutants are located in the phosphatase domain, which contains a number of surface-exposed residues conserved in predicted bacteriocins of other bacteria. (nih.gov)
- Like most bacteriocins produced by lactic acid bacteria, bovicin HJ50 showed a narrow range of inhibiting activity. (microbiologyresearch.org)
- Genetics of bacteriocins produced by lactic acid bacteria and their use in novel industrial applications. (microbiologyresearch.org)
- Here we briefly review the principal biopeptides (i.e., bacteriocins) of lactic acid bacteria, their main mode of action, the classification, and its biotechnological applications. (intechopen.com)
- Lactic acid bacteria (LAB) are among the most favorable microorganisms known for their probiotic properties and for the ability to produce antimicrobial compounds (i.e., bacteriocin, organic acids, diacetyl, hydrogen peroxide) with inhibitory action of harmful bacteria growth along with their critical role in food protection and health maintenance [ 1 - 3 ]. (intechopen.com)
- Nguyen Van Duy, Pham Thu Thuy, Nguyen Thi Hai Thanh, Nguyen Thi Thanh Xuan, Hoj Lone (2014): Screening of marine bacteria with bacteriocin-like activities and probiotic potential for ornate spiny lobster (Panulirus ornatus) juvenile. (duypost.com)
- Pham Thu Thuy, Ho Thi Hong Nhi, Nguyen Van Duy (2014): Screening for bacteriocin-like antimicrobial activity against shrimp pathogenic vibrios and molecular identification of marine bacteria from otter clam Lutraria philippinarum. (duypost.com)
- We can expect that the recent developments on bacteriocins from Lactic Acid Bacteria (LAB) described here will contribute greatly to increased commercialisation of bacteriocins in food systems. (teagasc.ie)
- Bacteriocin production is an important probiotic trait of intestinal bacteria. (teagasc.ie)
- During a screening of lactic acid bacteria producing bacteriocin from Cotija cheese, the strain QC38 was isolated. (pjmonline.org)
- Screening for bacteriocin-producing lactic acid bacteria. (pjmonline.org)
- The bacteriocins are modified at the ends of their tail fibers in a region responsible for binding specificity and affinity to their cognate binding partners, or receptors, such as those on the surface of bacteria. (patentsencyclopedia.com)
- Methods for the use of the modified bacteriocins, such as to bind receptors, including virulence or fitness factors, on the surfaces of bacteria, are also described. (patentsencyclopedia.com)
- The molecular mass of the purified lactolisterin BU was 5,160.94 Da, and an internal fragment, AVSWAWQH, as determined by N-terminal sequencing, showed low-level similarity to existing antimicrobial peptides. (ac.rs)
- With increasing antibiotic resistance and regulations regarding use, there is impetus to explore bacteriocins (bacterially produced antimicrobial peptides) for treatment and prevention of bacterial infections. (asm.org)
- Based on their biosynthetic mechanisms, the antimicrobial peptides are divided into two groups, the bacteriocins and the antibiotic peptides. (kowsarpub.com)
- Bacteriocins produced by LAB are a diverse group of ribosome-synthesized antimicrobial peptides that may be divided into two main groups: class I peptides, which contain post-translational modifications, and class II, or unmodified peptides (Rea et al . (pjmonline.org)
- class II bacteriocins are unmodified and include small, heat-stable nonlanthionine-containing peptides . (thefreedictionary.com)
- In the genome sequence of strain UA159 the muk locus is incomplete, the sole residual components being the ORFs encoding the putative two-component regulatory system mukR (SMU.1815) and mukK (SMU.1814), followed by two transposases (SMU.1813 and SMU.1812) and then the ORFs mukF (SMU.1811), mukE (SMU.1810) and mukG (SMU.1809), thought to encode putative immunity peptides. (microbiologyresearch.org)
- Lactococcin Q consisted of two peptides, α and β, whose molecular masses were determined to be 4,260.43 Da and 4,018.36 Da, respectively. (elsevier.com)
- One hundred five putative bacteriocin gene clusters encompassing 6 different classes (lanthipeptides, sactipeptides, lasso peptides, class IIa, class IIc, and class IId) in 95 whole genomes from 16 species were identified. (asm.org)
- Klaenhammer [ 8 ] suggested four classes of bacteriocins including class I or lantibiotics, which are small membrane-active peptides ( 5 kDa) containing the unusual amino acids lanthionine or β -methyl lanthionine (hence the name lantibiotics) and dehydrated residues. (hindawi.com)
- Class II is defined as small heat-stable nonlanthionine containing membrane active peptides characterized by the presence of a Gly-Gly processing site in the bacteriocins precursor, the presence of amphiphilic helices with varying amounts of hydrophobicity and moderate to high-heat stability. (hindawi.com)
- The N-terminal sequence of some peptides obtained after tryptic digestion has also been determined. (biomedsearch.com)
- Cloning and genetic and sequence analyses of the bacteriocin 21 determinant encoded on the Enterococcus faecalis pheromone-responsive conjugative plasmid pPD1. (asm.org)
- The pheromone-responsive conjugative plasmid pPD1 (59 kb) of Enterococcus faecalis encodes the bacteriocin 21 (bac21) determinant. (asm.org)
- The locations of the genetic determinants for enterocin L50 (EntL50A and EntL50B), enterocin Q (EntQ), and enterocin P (EntP) in the multiple bacteriocin producer Enterococcus faecium strain L50 were determined. (surrey.ac.uk)
- The sequence analysis of the 7383 bp plasmid pCIZ2 from Enterococcus faecium L50 enabled the identification of a DNA region involved in its replication. (archives-ouvertes.fr)
- A novel pathogenicity island in resistant Enterococcus faecalis has been sequenced. (ebi.ac.uk)
- The aim of this research was to purify and characterize the mode of action of enterocin S37, a bacteriocin produced by Enterococcus faecalis S37, a strain recently isolated from the chicken feces. (hindawi.com)
- They identified an R-plasmid (pJH1) and a hemolysin/ bacteriocin plasmid (pJH2) in a clinical isolate of Enterococcus (formerly Streptococcus ) faecalis and found both capable of transfer in broth matings (38, 39). (springer.com)
- Dysgalacticin: a novel, plasmid-encoded antimicrobial protein (bacteriocin) produced by Streptococcus dysgalactiae subsp. (microbiologyresearch.org)
- Oligonucleotides were constructed deduced from the peptide sequence and used for the identification of the curvacin A structural gene curA on a 60 kb plasmid of L. curvatus LTH1174. (nih.gov)
- Plasmid-cured derivatives of this strain were unable to produce curvacin A but were still resistant to the bacteriocin. (nih.gov)
- The complete nucleotide sequence of pETB, a 38.2-kb Staphylococcus aureus plasmid encoding the exfoliative toxin B (ETB), was determined. (asm.org)
- It showed 66% and 48% identity to AucA, a hypothetical protein from Corynebacterium jeikeium plasmid pA501, and aureocin A53, a bacteriocin from Staphylococcus aureus A53, respectively. (mysciencework.com)
- Curing and transformation experiments revealed the presence of a corresponding bacteriocin operon on the smallest plasmid, pBU6 (6.2 kb), of strain BGBU1-4. (ac.rs)
- Here, we have analyzed and compared the sequences of a large plasmid common to all sequenced Pantoea spp. (biomedcentral.com)
- The LPP-1 plasmid sequences range in size from ~281 to 794 kb and carry between 238 and 750 protein coding sequences (CDS). (biomedcentral.com)
- whose genomes have been sequenced to date and is derived from an ancestral plasmid. (biomedcentral.com)
- The deduced amino acid sequence showed significant homology to the C-terminal region of HlyB (31.1% identical residues), a protein located in the Escherichia coli alpha-hemolysin operon that is a representative bacterial ATP-binding cassette export protein. (asm.org)
- Bacteriocins are found in almost every bacterial species examined to date, and within a species, tens or even hundreds of different kinds of bacteriocins are produced. (asmscience.org)
- Multilocus sequence typing (MLST) is a robust and reliable method for characterising bacterial and fungal species at the molecular level. (beds.ac.uk)
- Traditional plating techniques are still being used for bacteriocin screening studies, however, the availability of ever more bacterial genome sequences and the use of in silico gene mining tools have revealed novel bacteriocin gene clusters that would otherwise have been overlooked. (teagasc.ie)
- The objective of this study was to determine and analyze the genome sequence of L. acidophilus NCFM, a probiotic culture that has been widely investigated for its physiological, biochemical, genetic, and fermentative properties ( 9 ). (pnas.org)
- The same principles apply to bacteriocin producing probiotic cultures and their application to control pathogens in the gut. (teagasc.ie)
- Sequencing revealed the presence of an additional open reading frame of 51 amino acids with unknown function. (nih.gov)
- Curvacin A is synthesised as a prepeptide of 59 amino acids which is proteolytically processed to the mature bacteriocin of 41 amino acids. (nih.gov)
- More specifically, lantibiotics are an important family of heat stable low molecular weight bacteriocins that possess unusual posttranslationally modified amino acids, such as lanthionine, methyllanthionine, dehydroalanine, and dehydrobutyrine with thioether linkages . (thefreedictionary.com)
- Class I consists of the lanthionine-containing post-translationally modified bacteriocins or lantibiotics, while class II consists of the bacteriocins containing non-modified amino acids. (biomedcentral.com)
- Since our publication of the annotated genomic sequence of the type species, the Spodoptera frugiperda ascovirus 1a (SfAV1a), we have made significant progress in elucidating the novel ultrastructure of this virus and in elucidating a mechanism for its genomic DNA condensation and encapsidation. (calbaptist.edu)
- Currently, traditional molecular typing approaches for characterisation of this species at the isolate level are either unavailable or are not sufficiently reliable for practical use. (beds.ac.uk)
- Interestingly, many of these were associated with species and genera which are not typically associated with bacteriocin production. (beds.ac.uk)
- AIMS: To characterize the genetic and biochemical features of nisin Q. METHODS AND RESULTS: The nisin Q gene cluster was sequenced, and 11 putative orfs having 82% homology with the nisin A biosynthesis gene cluster were identified. (biomedsearch.com)
- Improvements brought to BACTIBASE include incorporation of various tools for bacteriocin analysis, such as homology search, multiple sequence alignments, Hidden Markov Models, molecular modelling and retrieval through our taxonomy Browser. (biomedcentral.com)
- Nguyen Chuong, Nguyen Van Duy (2016): Discovery of azurin-like anticancer bacteriocins from human gut microbiome through homology modeling and molecular docking against the tumor suppressor p53. (duypost.com)
- Moreover, we used different cell-biological and animal experiments to compare the isolate D32 with a closely related ST40 endocarditis isolate whose draft genome sequence was also generated. (biomedcentral.com)
- Complete Genome Sequence of Pseudomonas aeruginosa K34-7, a Carbapenem-Resistant Isolate of the High-Risk Sequence Type 233. (uio.no)
- Intraspecific and within-isolate sequence variation in the ITS rRNA gene region of Pythium mercuriale sp. (sun.ac.za)
- Sequence analysis indicated 20 different sequence types (STs), with 13 of them being represented by a single isolate. (beds.ac.uk)
- Based on the 16S rRNA gene nucleotide sequencing (516 pb accession no KJ210322) and phylogenetic analysis, the isolate was identified as Pediococcus acidilactici . (pjmonline.org)
- The bacteriocin produced by the isolate were also characterized. (pjmonline.org)
- Lactococcus lactis QU 5 isolated from corn produces a novel bacteriocin, termed lacticin Q. By acetone precipitation, cation-exchange chromatography, and reverse-phase high-performance liquid chromatography, lacticin Q was purified from the culture supernatant of this organism, and its molecular mass was determined to be 5,926.50 Da by mass spectrometry. (mysciencework.com)
- The deduced amino acid sequence of the putative biosynthetic gene penA showed approximately 74% similarity with elgicin A and 50% similarity with the lantibiotic paenicidin A. Penisin effectively killed methicillin-resistant Staphylococcus aureus (MRSA) and did not exhibit hemolysis activity. (osdd.net)
- Two main features distinguish the majority of bacteriocins from conventional antibiotics: bacteriocins are ribosomally synthesized and have a relatively narrow killing spectrum (3). (biomedcentral.com)
- Those synthesized in the ribosomes, are generally referred to as bacteriocins (antibiotics are not included in the group since they are not ribosomally synthesized). (biomedcentral.com)
- As regulations surrounding antibiotic usage get stricter, bacteriocins represent potential alternatives to antibiotics ( 10 ). (asm.org)
- 6,7 Notably, ASM1, a close homologue of GccF, and enterocin 96 are the only glycosylated bacteriocins which have been shown to be "glycoactive", 11-13 that is, the saccharide moieties are essential for biological activity. (rsc.org)
- Enterocin K1 (EntK1), enterocin EJ97 (EntEJ97), and LsbB are three sequence related leaderless bacteriocins. (uio.no)
- Enterocin S37 has a molecular weight comprised between 4 and 5 kDa. (hindawi.com)
- Nine two-component regulatory systems were predicted, some associated with determinants implicated in bacteriocin production and acid tolerance. (pnas.org)
- Halocins are particularly interesting because of the way the pore-forming bacteriocins have been used to probe cell membrane structure and the production and maintenance of energetic ion gradients across the membrane. (wikipedia.org)
- We found a high levels of bacteriocin production for both genera, these frequencies were higher in asymptomatic and tangerine plants. (usp.br)
- PlnA-22L induced bacteriocin production in L. plantarum C11 (i.e., displayed pheromone activity), the level of induction being clearly dose-dependent. (nih.gov)
- It plays an important role in the food industry including the production of dextrans and bacteriocins. (beds.ac.uk)
- Nguyen Van Duy, Nguyen Thi Ngoc Thanh (2012): Bacteriocin production by Proteus sp. (duypost.com)
- Production of bacteriocin-like substances by group D streptococci of human origin. (edu.au)
- In silico -based approaches to identify novel gene clusters are now also being utilised to take advantage of the vast amount of data currently being generated by next generation sequencing technologies. (beds.ac.uk)
- Multiple bacteriocin gene clusters were identified in a dataset representative of the human gut microbiota. (beds.ac.uk)