Bacteriocins: Substances elaborated by specific strains of bacteria that are lethal against other strains of the same or related species. They are protein or lipopolysaccharide-protein complexes used in taxonomy studies of bacteria.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Evolution, Molecular: The process of cumulative change at the level of DNA; RNA; and PROTEINS, over successive generations.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Bayes Theorem: A theorem in probability theory named for Thomas Bayes (1702-1761). In epidemiology, it is used to obtain the probability of disease in a group of people with some characteristic on the basis of the overall rate of that disease and of the likelihood of that characteristic in healthy and diseased individuals. The most familiar application is in clinical decision analysis where it is used for estimating the probability of a particular diagnosis given the appearance of some symptoms or test result.Pediococcus: A genus of gram-positive, facultatively anaerobic bacteria whose growth is dependent on the presence of a fermentable carbohydrate. No endospores are produced. Its organisms are found in fermenting plant products and are nonpathogenic to plants and animals, including humans.Lactobacillus: A genus of gram-positive, microaerophilic, rod-shaped bacteria occurring widely in nature. Its species are also part of the many normal flora of the mouth, intestinal tract, and vagina of many mammals, including humans. Pathogenicity from this genus is rare.Pyocins: Bacteriocins elaborated by mutant strains of Pseudomonas aeruginosa. They are protein or protein-lipopolysaccharide complexes lethal to other strains of the same or related species.Likelihood Functions: Functions constructed from a statistical model and a set of observed data which give the probability of that data for various values of the unknown model parameters. Those parameter values that maximize the probability are the maximum likelihood estimates of the parameters.Models, Genetic: Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.Fossils: Remains, impressions, or traces of animals or plants of past geological times which have been preserved in the earth's crust.Nisin: A 34-amino acid polypeptide antibiotic produced by Streptococcus lactis. It has been used as a food preservative in canned fruits and vegetables, and cheese.Software: Sequential operating programs and data which instruct the functioning of a digital computer.Listeria: A genus of bacteria which may be found in the feces of animals and man, on vegetation, and in silage. Its species are parasitic on cold-blooded and warm-blooded animals, including man.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Markov Chains: A stochastic process such that the conditional probability distribution for a state at any future instant, given the present state, is unaffected by any additional knowledge of the past history of the system.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Lactobacillaceae: A family of gram-positive bacteria found regularly in the mouth and intestinal tract of man and other animals, in food and dairy products, and in fermenting vegetable juices. A few species are highly pathogenic.RNA, Ribosomal, 18S: Constituent of the 40S subunit of eukaryotic ribosomes. 18S rRNA is involved in the initiation of polypeptide synthesis in eukaryotes.Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.Biological Evolution: The process of cumulative change over successive generations through which organisms acquire their distinguishing morphological and physiological characteristics.Gram-Positive Asporogenous Rods: A gram-positive, non-spore-forming group of bacteria comprising organisms that have morphological and physiological characteristics in common.Antibiosis: A natural association between organisms that is detrimental to at least one of them. This often refers to the production of chemicals by one microorganism that is harmful to another.Lactococcus: A genus of gram-positive, coccoid bacteria mainly isolated from milk and milk products. These bacteria are also found in plants and nonsterile frozen and dry foods. Previously thought to be a member of the genus STREPTOCOCCUS (group N), it is now recognized as a separate genus.Colicins: Bacteriocins elaborated by strains of Escherichia coli and related species. They are proteins or protein-lipopolysaccharide complexes lethal to other strains of the same species.Monte Carlo Method: In statistics, a technique for numerically approximating the solution of a mathematical problem by studying the distribution of some random variable, often generated by a computer. The name alludes to the randomness characteristic of the games of chance played at the gambling casinos in Monte Carlo. (From Random House Unabridged Dictionary, 2d ed, 1993)Gram-Positive Bacteria: Bacteria which retain the crystal violet stain when treated by Gram's method.Computational Biology: A field of biology concerned with the development of techniques for the collection and manipulation of biological data, and the use of such data to make biological discoveries or predictions. This field encompasses all computational methods and theories for solving biological problems including manipulation of models and datasets.Lactobacillus acidophilus: A species of gram-positive, rod-shaped bacteria isolated from the intestinal tract of humans and animals, the human mouth, and vagina. This organism produces the fermented product, acidophilus milk.Lactococcus lactis: A non-pathogenic species of LACTOCOCCUS found in DAIRY PRODUCTS and responsible for the souring of MILK and the production of LACTIC ACID.DNA, Mitochondrial: Double-stranded DNA of MITOCHONDRIA. In eukaryotes, the mitochondrial GENOME is circular and codes for ribosomal RNAs, transfer RNAs, and about 10 proteins.DNA, Ribosomal: DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.Leuconostoc: A genus of gram-positive, facultatively anaerobic bacteria whose growth is dependent on the presence of a fermentable carbohydrate. It is nonpathogenic to plants and animals, including humans.Enterococcus faecium: A species of gram-positive, coccoid bacteria whose organisms are normal flora of the intestinal tract. Unlike ENTEROCOCCUS FAECALIS, this species may produce an alpha-hemolytic reaction on blood agar and is unable to utilize pyruvic acid as an energy source.Listeria monocytogenes: A species of gram-positive, rod-shaped bacteria widely distributed in nature. It has been isolated from sewage, soil, silage, and from feces of healthy animals and man. Infection with this bacterium leads to encephalitis, meningitis, endocarditis, and abortion.Computer Simulation: Computer-based representation of physical systems and phenomena such as chemical processes.Genetic Variation: Genotypic differences observed among individuals in a population.Streptococcus mutans: A polysaccharide-producing species of STREPTOCOCCUS isolated from human dental plaque.Enterococcus faecalis: A species of gram-positive, coccoid bacteria commonly isolated from clinical specimens and the human intestinal tract. Most strains are nonhemolytic.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Hafnia alvei: The type species for the genus HAFNIA. It is distinguished from other biochemically similar bacteria by its lack of acid production on media containing sucrose. (From Bergey's Manual of Determinative Bacteriology, 9th ed)Microbial Sensitivity Tests: Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).Streptococcus: A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Yogurt: A slightly acid milk food produced by fermentation due to the combined action of Lactobacillus acidophilus and Streptococcus thermophilus.Clostridium perfringens: The most common etiologic agent of GAS GANGRENE. It is differentiable into several distinct types based on the distribution of twelve different toxins.Enterococcus: A genus of gram-positive, coccoid bacteria consisting of organisms causing variable hemolysis that are normal flora of the intestinal tract. Previously thought to be a member of the genus STREPTOCOCCUS, it is now recognized as a separate genus.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Genes, Bacterial: The functional hereditary units of BACTERIA.Bacterial Proteins: Proteins found in any species of bacterium.Food Preservation: Procedures or techniques used to keep food from spoiling.Mitomycins: A group of methylazirinopyrroloindolediones obtained from certain Streptomyces strains. They are very toxic antibiotics used as ANTINEOPLASTIC AGENTS in some solid tumors. PORFIROMYCIN and MITOMYCIN are the most useful members of the group.Bacteriophages: Viruses whose hosts are bacterial cells.Spheroplasts: Cells, usually bacteria or yeast, which have partially lost their cell wall, lost their characteristic shape and become round.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Bacillus: A genus of BACILLACEAE that are spore-forming, rod-shaped cells. Most species are saprophytic soil forms with only a few species being pathogenic.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
... s are bacteriocins produced by halophilic Archaea and a type of archaeocin. Since their discovery in 1982, halocins have ... Cheung J, Danna KJ, O'Connor EM, Price LB, Shand RF (1997). "Isolation, sequence, and expression of the gene encoding halocin ... Molecular Microbiology. 57 (2): 537-49. doi:10.1111/j.1365-2958.2005.04705.x. PMID 15978083. Perez, AM (2000). Growth ... Like other bacteriocins, the halocins are under investigation as antimicrobials for use in controlling spoilage during ...
These enzymes became known as restriction enzymes and developed into one of the main molecular tools in molecular biology. In ... he found that bacteriocins impair the function of cell membranes. Returning to MIT, his lab discovered that bacteriocins ... It was later discovered by other researchers that bacteria produce enzymes that cut viral DNA at particular sequences but not ... Roberts RJ (April 2005). "How restriction enzymes became the workhorses of molecular biology". Proc. Natl. Acad. Sci. U.S.A. ...
Comprehensive sequence searches across multiple taxonomic groups have failed to identify MHC and TCRs outside of the jawed ... For other articles which focus on its importance in medicine, molecular biology, and so forth, the following topics are ... Bacteria, for instance, secrete bacteriocins, proteinaceous toxins specifically targeted against members of their own species. ... Interestingly, sequence variability in "hot spots" have been identified in these receptors. It would appear that molecules ...
"Analysis of the Genome Sequence of Lactobacillus gasseri ATCC 33323 Reveals the Molecular Basis of an Autochthonous Intestinal ... 1]Pandey N, Malik RK, Kaushik JK, Singroha G (2013). "Gassericin A: a circular bacteriocin produced by lactic acid bacteria ... Its genome has been sequenced. L. gasseri is a normal inhabitant of the lower reproductive tract in healthy women. It also ... L. gasseri produces gassericin A, a bacteriocin. Lauer, Eckhard, and Otto Kandler. " Lactobacillus gasseri sp. nov., a new ...
February 2011). "Genome Sequence of Weissella cibaria KACC 11862". Journal of Bacteriology. 193 (3): 797-8. doi:10.1128/JB. ... Srionnual, S.; Yanagida, F.; Lin, L.-H.; Hsiao, K.-N.; Chen, Y.-s. (2007). "Weissellicin 110, a Newly Discovered Bacteriocin ... W.cibaria CMGDEX3 was reported from Pakistan to produce high molecular weight,linear dextran with predominant (1→6) linkages. ... 2012). "Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3". Carbohydrate Polymers. 90: ...
Most of the molecular mechanisms are not known, however. L. rhamnosus GG is beneficial in the prevention of rotavirus diarrhea ... The genome sequence of Lactobacillus rhamnosus GG (ATCC 53103) has been decoded. In 1983, Lactobacillus rhamnosus GG was ... bacteriocin production, pili production, the cassystem, the clustered regularly interspaced short palindromic repeat (CRISPR) ... Morita H, Toh H, Oshima K, Murakami M, Taylor TD, Igimi S, Hattori M (December 2009). "Complete genome sequence of the ...
Bacteriocin genes are located either on chromosoms or on plasmids. Strain 5 produces a plasmid-encoded bacteriocin that is ... Mørtvedt, C. I.; Nissen-Meyer, J.; Sletten, K.; Nes, I. F. (1991). "Purification and amino acid sequence of lactocin S, a ... 4, pages 1359-1365 (abstract) Schmidt, G.; Hertel, C.; Hammes, W. P. (1999). "Molecular Characterisation of the dnaK Operon of ... Sakacins are bacteriocins of class II produced by L. sakei. In strain CCUG 42687, their production is dependent on nutrients, ...
The porins act as molecular filters for hydrophilic compounds. They are responsible for the 'molecular sieve' properties of the ... Structural and sequence analysis provide firm evidence that the GBP, SP and RPP families together with 44 additional families ... they also serve as receptor sites for the binding of phages and bacteriocins. General diffusion porins usually assemble as a ... Pattus F, Jeanteur D, Lakey JH (1991). "The bacterial porin superfamily: sequence alignment and structure prediction". Mol. ...
The molecular mass of the mature HalH4 protein is 34.9 kDa (359 amino acids), processed from a preprotein of 39.6 kDa; the ... Cheung J; Danna KJ; O'Connor EM; Price LB; Shand RF (1997). "Isolation, sequence, and expression of the gene encoding halocin ... H4, a bacteriocin from the halophilic archaeon Haloferax mediterranei R4". J. Bacteriol. 179 (2): 548-51. PMC 178729 . PMID ... These antimicrobial peptides range from ~3 to 7.4 kDa in molecular mass, consisting of 36 to 76 amino acid residues. Two of the ...
High-throughput sequencing and the whole genome sequencing approaches will provide the further information about the complexity ... Human microbiome List of human flora Bacteriocin Erb-Downward, John R.; Thompson, Deborah L.; Han, Meilan K.; Freeman, ... journal of molecular epidemiology and evolutionary genetics in infectious diseases. 21: 563-74. doi:10.1016/j.meegid.2013.04. ... The DNA-binding protein complex recognizes a discrete nucleotide sequence (5'-GGG ACT TTC T-3') in the upstream region of a ...
Some of the Corynebacterium species with sequenced genomes have between 2.5 and 3.0 million base pairs. The bacteria grow in ... Kerry-Williams, S.M.; Noble, W.C. (1984). "Plasmid-associated bacteriocin production in a JK-type coryneform bacterium". FEMS ... Gao, B.; Gupta, R. S. (2012). "Phylogenetic Framework and Molecular Signatures for the Main Clades of the Phylum Actinobacteria ... In phylogenetic trees based on concatenated protein sequences or 16S rRNA, the genus Corynebacterium forms a distinct clade, ...
sequenced the genome of IL1403, which coincided with a significant shift of resources to understanding LAB genomics and related ... Proteinaceous bacteriocins are produced by several LAB strains and provide an additional hurdle for spoilage and pathogenic ... However, there is a wide variation in molecular structures of exopolysaccharides and the complexity of the mechanisms by which ... Mc Grath S and van Sinderen D (editors). (2007). Bacteriophage: Genetics and Molecular Biology (1st ed.). Caister Academic ...
Provides information on molecular mass, composition, sequence, and sometimes branching of a glycan chain. It can also be used ... a new post-translational modification found in glycopeptide bacteriocins". FEBS Letters. 585 (4): 645-650. doi:10.1016/j. ... To identify specific sugars, their sequence, linkages, and the anomeric nature of glycosidic chain. ... provides information on molecular mass, protein-carbohydrate ratio, aggregation state, size, and sometimes branching of a ...
... and tRNA-Ala genes at 403 base pairs for the complete sequence. Other genes that have been sequenced include the following: ... Gilberts, EC; Greenstein, AJ; Katsel, P; Harpaz, N; Greenstein, RJ (1994). "Molecular evidence for two forms of Crohn disease ... Finally, they produce antimicrobial compounds, such as organic acids (lactate, acetate, and others), bacteriocins, and ... L. Pontis was classified as a new species based on the 16S rRNA gene sequence. Strain LTH 2587T, also known as DSM 8475/ LMG ...
It shows significant sequence similarity to human ABC transporters of subfamily B that includes MDR1 and TAP1/TAP2. The ATPase ... The precise molecular mechanism of ATP hydrolysis is still controversial. ABC transporters are active transporters, that is, ... One class is involved in the protein (e.g. toxins, hydrolytic enzymes, S-layer proteins, lantibiotics, bacteriocins, and ... The sequence and architecture of TMDs is variable, reflecting the chemical diversity of substrates that can be translocated. ...
Research teams have sequenced the genome of two strains of S. thermophilus, CNRZ1066 and LMG13811, and stated that the bacteria ... Kiliç, AO; Pavlova, SI; Ma, WG; Tao, L (1996). "Analysis of Lactobacillus phages and bacteriocins in American dairy products ... "Mixed-Culture Transcriptome Analysis Reveals the Molecular Basis of Mixed-Culture Growth in Streptococcus thermophilus and ... At least 26 strains of S. thermophilis have been identified and had their genomes sequenced. S. thermophilus is one of the most ...
The genome is composed of ~15-kb linear double-stranded DNA (dsDNA) with long inverted terminal repeat sequences (􏰵100 bp). ... Naclerio, G; Ricca, E; Sacco, M; De Felice, M (December 1993). "Antimicrobial activity of a newly identified bacteriocin of ... Molecular Microbiology. 59 (5): 1602-9. doi:10.1111/j.1365-2958.2006.05044.x. PMC 1413580 . PMID 16468998. Vilà, B.; Fontgibell ... Some strains of B. cereus produce cereins, bacteriocins active against different B. cereus strains or other Gram-positive ...
... colicin-like bacteriocins are 20 to 90 kDa in size and tailocins or so called high molecular weight bacteriocins which are ... Mørtvedt, C. I.; Nissen-Meyer, J.; Sletten, K.; Nes, I. F. (1991). "Purification and amino acid sequence of lactocin S, a ... They are the longest studied bacteriocins. They are a diverse group of bacteriocins and do not include all the bacteriocins ... The class I bacteriocins are small peptide inhibitors and include nisin and other lantibiotics. The class II bacteriocins are ...
The lantibiotics are a class of more extensively modified bacteriocins, also called Class I bacteriocins. (Bacteriocins for ... They are synthesised with a leader polypeptide sequence that is removed only during the transport of the molecule out of the ... "New insights into the mechanism of action of lantibiotics-diverse biological effects by binding to the same molecular target". ... they are considered a member of the bacteriocins. Bacteriocins are classified according to their extent of posttranslational ...
... were fully sequenced. A. tumefaciens C58, the first fully sequenced pathovar, was first isolated from a cherry tree crown gall ... This biosynthetic pathway is not used in many plants for the production of auxin, so it means the plant has no molecular means ... K84 produces a bacteriocin (agrocin 84) which is an antibiotic specific against related bacteria, including A. tumefaciens.This ... This is done by cloning a desired gene sequence into the T-DNA that will be inserted into the host DNA. This process has been ...
Researchers also often upload plasmid sequences to the NCBI database, from which sequences of specific plasmids can be ... "Microbiology and Molecular Biology Reviews. 74 (3): 434-52. doi:10.1128/MMBR.00020-10. PMC 2937521. PMID 20805406.. ... Col plasmids, which contain genes that code for bacteriocins, proteins that can kill other bacteria. ... "Current Opinion in Molecular Therapeutics. 11 (4): 433-41. PMID 19649988. Archived from the original on 17 September 2011.. ...
2009). Lactobacillus Molecular Biology: From Genomics to Probiotics. Caister Academic Press. ISBN 978-1904455417. [page needed ... Recent developments of high-throughput sequencing technology and the consequent progresses of metagenomics represent a new ... and characterization of two bacteriocins produced by isolates of Enterococcus faecium". Journal of Applied Microbiology. The ... Research into the potential health effects of supplemental probiotics has included the molecular biology and genomics of ...
A method for promoting plant growth and/or disease resistance comprising applying a purified polypeptide that is a bacteriocin ... The molecular weight of the ion for sequencing was slightly less than the initially determined molecular weight of 3162 Da ( ... Many but not all bacteriocins are of low-molecular weight, in the range of about 100 to about 10,000 Daltons. Bacteriocins are ... Furthermore, the sequence information for Plantaricin S, a bacteriocin produced from Lactobacillus plantarum LPCO10 requiring ...
MALDI-TOF experiments determined the precise molecular mass of the peptide to be of 5.6 kDa and N-terminal sequencing of the ... Sequence analysis of the putative bacteriocin gene did not show significant similarity to any reported bacteriocin producing ... by 16 S rRNA gene sequence analysis. The bacteriocin produced by strain GI-9, namely, laterosporulin was purified from ... The bacteriocin was active against a wide range of Gram-positive and Gram-negative bacteria. ...
... bacH encoded a 195-residue sequence with a molecular mass of 21.9 kDa. The deduced amino acid sequence showed significant ... bacA encoded a 105-residue sequence with a molecular mass of 11.1 kDa. The deduced BacA protein showed 100% homology to the ... The smallest fragment (4.5 kb) retaining some degree of bacteriocin expression contained the bacA to bacE sequences located in ... Cloning and genetic and sequence analyses of the bacteriocin 21 determinant encoded on the Enterococcus faecalis pheromone- ...
The nisin Q gene cluster was sequenced, and 11 putative orfs having 82% homology with the nisin A biosynthesis gene cluster ... Molecular Sequence Data. Multigene Family / genetics. Nisin / analogs & derivatives*, biosynthesis, genetics, pharmacology. ... Bacteriocins / genetics*, metabolism, pharmacology. DNA, Bacterial / genetics. Lactococcus lactis / genetics*, metabolism. ... The nisin Q gene cluster was sequenced, and 11 putative orfs having 82% homology with the nisin A biosynthesis gene cluster ...
... www.uniprot.org/help/sequences">molecular weight,/a>. The information is filed in different subsections. The current ... IPR009086, Bacteriocin_AS48. ,p>This section displays by default the canonical protein sequence and upon request all isoforms ... Sequence databases. Select the link destinations:. EMBL nucleotide sequence database. More...EMBLi. GenBank nucleotide sequence ... p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates ...
Non-coding RNAs in Gram postive bacteria; bacteriocins. Dr Daniel Mitchell Molecular basis for immune system interactions with ... Bacteriology; antibiotic resistance; mobile genetic elements; transcriptomics; next-generation sequencing; Haemophilus ... Bioinformatics and laboratory-based molecular bacteriology.. Professor Matthew Gibson Organic, polymer and carbohydrate ...
Amino Acid Sequence, Bacteriocins, Base Sequence, DNA, Bacterial, Enterococcus faecium, Molecular Sequence Data, Open Reading ... Complete sequence of the enterocin Q-encoding plasmid pCIZ2 from the multiple bacteriocin producer Enterococcus faecium L50 and ... Complete sequence of the enterocin Q-encoding plasmid pCIZ2 from the multiple bacteriocin producer Enterococcus faecium L50 and ... in the multiple bacteriocin producer Enterococcus faecium strain L50 were determined. These bacteriocin genes occur at ...
Research Topics about Experts and Doctors on molecular sequence data in Edmonton, Alberta, Canada ... Subtilosin A (1) represents a new class of bacteriocins. ... *. Lu J, Edwards R, Wong J, Manchak J, Scott P, Frost L, et al. ... Experts and Doctors on molecular sequence data in Edmonton, Alberta, Canada. Summary. Locale: Edmonton, Alberta, Canada ... You are here: Locale , Canada , Alberta , Experts and Doctors on molecular sequence data in Edmonton, Alberta, Canada ...
Lactacin B is a class II bacteriocin produced by L. acidophilus NCFM (24). The genome sequence of L. acidophilus NCFM ... PNAS Profile of NAS member and molecular biologist Rodolphe Barrangou. Image courtesy of North Carolina State University/Marc ... Sequence repeat lengths, in bp, are displayed by color coding as indicated by the colored boxes. The sequence 5′- ... M.C. and S.L. contributed to manual annotation; A.H. and R.C. provided DNA sequence and assembly data. Contract sequencing for ...
Amino Acid Sequence · Bacteriocins · Base Sequence · Cloning, Molecular · Codon, Initiator · Codon, Terminator · DNA, Bacterial ... DNA sequence · genetic code · Lactobacillus gasseri · molecular cloning · nonhuman · nucleotide sequence · open reading frame ... Standard procedures for molecular techniques were used to locate, clone and sequence the fragments of LF221 chromosomal DNA ... Molecular weight · Mouth disease · Nonhuman · Obesity · Pyrosequencing · Respiratory tract infection · Review · RNA sequence · ...
These enzymes became known as restriction enzymes and developed into one of the main molecular tools in molecular biology. In ... he found that bacteriocins impair the function of cell membranes. Returning to MIT, his lab discovered that bacteriocins ... It was later discovered by other researchers that bacteria produce enzymes that cut viral DNA at particular sequences but not ... Roberts RJ (April 2005). "How restriction enzymes became the workhorses of molecular biology". Proc. Natl. Acad. Sci. U.S.A. ...
The N-terminal amino acid sequence of dysgalacticin [Asn-Glu-Thr-Asn-Asn-Phe-Ala-Glu-Thr-Gln-Lys-Glu-Ile-Thr-Thr-Asn-(Asn)-Glu- ... Unlike many previously described bacteriocins of Gram-positive bacteria, dysgalacticin is a heat-labile 21.5 kDa anionic ... Natural dysA variants containing conservative amino acid substitutions were also detected by sequence analyses of dysA elements ... Moreover, dysgalacticin displays similarity in predicted secondary structure (but not primary amino acid sequence or inhibitory ...
Halocins are bacteriocins produced by halophilic Archaea and a type of archaeocin. Since their discovery in 1982, halocins have ... Cheung J, Danna KJ, OConnor EM, Price LB, Shand RF (1997). "Isolation, sequence, and expression of the gene encoding halocin ... Molecular Microbiology. 57 (2): 537-49. doi:10.1111/j.1365-2958.2005.04705.x. PMID 15978083. Perez, AM (2000). Growth ... Like other bacteriocins, the halocins are under investigation as antimicrobials for use in controlling spoilage during ...
MOLECULAR BIOLOGY OF PATHOGENS. Role of Bacteriocin Immunity Proteins in the Antimicrobial Sensitivity of Streptococcus mutans ... an analysis revealed that the replacement of the comC gene with an Ermr gene also removed a portion of the nucleotide sequence ... mutans GS5 is modulated by some of the putative bacteriocin immunity proteins expressed by the organism. Since the bacteriocin ... A role for bacteriocin immunity proteins in resistance to some noncognate bacteriocins was suggested by the demonstration that ...
We compared a set of 42 ST40 isolates by assessing key molecular determinants, performing whole genome sequencing (WGS) and a ... Molecular, genomic and phenotypic analysis of representative isolates of a major clone of E. faecalis MLST ST40 revealed new ... and animal experiments to compare the isolate D32 with a closely related ST40 endocarditis isolate whose draft genome sequence ... because of their production of secondary metabolites and bacteriocins [5]. On the other hand, enterococci, in particular E. ...
In silico analyses suggested that endophycin L is a toxin-like bacteriocin, this bacteriocin could be a potential candidate as ... A endoficina L parcialmente purificada apresentou um peso molecular estimado de 14 kDa e atividade in gel, e atingiu seu nível ... flaccumfaciens was selected for its bacteriocinogenic ability The fragment was subcloned and DNA sequencing revealed a 2-kb ... Bacteriocins are antimicrobial proteins produced by bacteria, which play a role in microbial ecology. In this work we examined ...
Structure (Molecular Modeling Database). *Vector Alignment Search Tool (VAST). *All Domains & Structures Resources... ... Cloning and sequencing of curA encoding curvacin A, the bacteriocin produced by Lactobacillus curvatus LTH1174.. Tichaczek PS1 ... CurA was cloned into Escherichia coli NM554 and its nucleotide sequence was determined. Sequencing revealed the presence of an ... Curvacin A is synthesised as a prepeptide of 59 amino acids which is proteolytically processed to the mature bacteriocin of 41 ...
Mass Spectrometry and Amino Acid Sequence. The molecular mass of the purified pentocin JL-1 was detected by MALDI-TOF-MS ( ... Many bacteriocins such as bacteriocin KU24 [25], bacteriocin VJ13 [37], plantaricin ZJ5 [13], and other LAB bacteriocins share ... Purification of the Bacteriocin. The bacteriocin, pentocin JL-1, is one of the secondary metabolites of L. pentosus JL-1 after ... The 16S rRNA gene sequence was amplified by PCR, and a 1485 bp gene fragment was sequenced after cloning and aligned using the ...
The molecular mass of the purified protein is 24 kDa (Fig. 4a), and the N-terminal sequence, DDVKN, exactly matched the ... Sequence and analysis of the 60 kb conjugative, bacteriocin-producing plasmid pMRC01 from Lactococcus lactis DPC3147.Mol. ... Collected sequences were assembled by using the SEQUENCHER DNA sequencing software (v.3.0; Gene Codes). The sequences of both ... Molecular Pathogenesis. Complete Nucleotide Sequence of aStaphylococcus aureus Exfoliative Toxin B Plasmid and Identification ...
were isolated and subsequently identified based on biochemical and molecular methods. According to the sequencing of isolates, ... The specific primers of bacteriocin used in this study are shown in Table 1. According to the bacteriocins sequences of ... and M12 had bre174A bacteriocin gene (Fig. 4; Table 7). Sequence and culture deposition Sequences were submitted to the NCBI ... were isolated and subsequently identified based on biochemical and molecular methods. According to the sequencing of isolates, ...
Molecular Sequence Data * Peptides* * Peptides, Cyclic / isolation & purification * Peptides, Cyclic / pharmacology * ... Bacteriocins * Chromatography, High Pressure Liquid * Chromatography, Ion Exchange * Gas Chromatography-Mass Spectrometry ... of the polypeptide containing four thioether bridges involved mainly a combination of automated gas-phase sequencing, ...
Molecular Sequence Data. *Pheromones/biosynthesis. *Pheromones/chemistry*. *Pheromones/physiology. *Protein Structure, ... Production of bacteriocins by lactic acid bacteria is in some cases regulated by a quorum sensing mechanism that involves a ... PlnA-22L induced bacteriocin production in L. plantarum C11 (i.e., displayed pheromone activity), the level of induction being ... To gain insight into how PlnA functions as a pheromone and as a bacteriocin, the L- and D-enantiomers of an N-terminally ...
GccF is a rare example of a glycoactive bacteriocin, with both the O-linked N-acetylglucosamine (GlcNAc) and the unusual S- ... cMaurice Wilkins Centre for Molecular Biodiscovery, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand d ... Strikingly, the bioactivity increased significantly when the sequence incorporated two S-GlcNAc moieties at positions 18 and 43 ... Glycocin F (GccF, 1) is a potent bacteriocin originally isolated from liquid culture of Lactobacillus plantarum KW30.6 GccF is ...
... bacteriocins; molecular weight; nucleotide sequences; polyacrylamide gel electrophoresis; polymerase chain reaction; rumen; ... Bacteriocin produced by E. faecalis II/1 was purified to homogeneity. Purified bacteriocin exhibited a single band on sodium ... Purification and partial characterization of an extracellular bacteriocin produced by the ruminal isolate Enterococcus faecalis ... DNA; Enterococcus faecalis; Gram-positive bacteria; amino acid sequences; amino acids; antibacterial proteins; ...
  • class II bacteriocins are unmodified and include small, heat-stable nonlanthionine-containing peptides . (thefreedictionary.com)
  • In the genome sequence of strain UA159 the muk locus is incomplete, the sole residual components being the ORFs encoding the putative two-component regulatory system mukR (SMU.1815) and mukK (SMU.1814), followed by two transposases (SMU.1813 and SMU.1812) and then the ORFs mukF (SMU.1811), mukE (SMU.1810) and mukG (SMU.1809), thought to encode putative immunity peptides. (microbiologyresearch.org)
  • Lactococcin Q consisted of two peptides, α and β, whose molecular masses were determined to be 4,260.43 Da and 4,018.36 Da, respectively. (elsevier.com)
  • One hundred five putative bacteriocin gene clusters encompassing 6 different classes (lanthipeptides, sactipeptides, lasso peptides, class IIa, class IIc, and class IId) in 95 whole genomes from 16 species were identified. (asm.org)
  • Klaenhammer [ 8 ] suggested four classes of bacteriocins including class I or lantibiotics, which are small membrane-active peptides ( 5 kDa) containing the unusual amino acids lanthionine or β -methyl lanthionine (hence the name lantibiotics) and dehydrated residues. (hindawi.com)
  • Class II is defined as small heat-stable nonlanthionine containing membrane active peptides characterized by the presence of a Gly-Gly processing site in the bacteriocins precursor, the presence of amphiphilic helices with varying amounts of hydrophobicity and moderate to high-heat stability. (hindawi.com)
  • The N-terminal sequence of some peptides obtained after tryptic digestion has also been determined. (biomedsearch.com)
  • The objective of this study was to determine and analyze the genome sequence of L. acidophilus NCFM, a probiotic culture that has been widely investigated for its physiological, biochemical, genetic, and fermentative properties ( 9 ). (pnas.org)
  • The same principles apply to bacteriocin producing probiotic cultures and their application to control pathogens in the gut. (teagasc.ie)
  • Sequencing revealed the presence of an additional open reading frame of 51 amino acids with unknown function. (nih.gov)
  • Curvacin A is synthesised as a prepeptide of 59 amino acids which is proteolytically processed to the mature bacteriocin of 41 amino acids. (nih.gov)
  • More specifically, lantibiotics are an important family of heat stable low molecular weight bacteriocins that possess unusual posttranslationally modified amino acids, such as lanthionine, methyllanthionine, dehydroalanine, and dehydrobutyrine with thioether linkages . (thefreedictionary.com)
  • Class I consists of the lanthionine-containing post-translationally modified bacteriocins or lantibiotics, while class II consists of the bacteriocins containing non-modified amino acids. (biomedcentral.com)
  • Since our publication of the annotated genomic sequence of the type species, the Spodoptera frugiperda ascovirus 1a (SfAV1a), we have made significant progress in elucidating the novel ultrastructure of this virus and in elucidating a mechanism for its genomic DNA condensation and encapsidation. (calbaptist.edu)
  • Currently, traditional molecular typing approaches for characterisation of this species at the isolate level are either unavailable or are not sufficiently reliable for practical use. (beds.ac.uk)
  • Interestingly, many of these were associated with species and genera which are not typically associated with bacteriocin production. (beds.ac.uk)
  • Moreover, we also obtained experimental results indicating that EntqB is involved in ABC transporter-mediated EntQ secretion, while EntqC confers immunity to this bacteriocin. (surrey.ac.uk)
  • This study focused on the development of molecular tools for the rapid detection and characterisation of food-borne pathogens including Verocytotoxigenic Escherichia coli (VTEC) (serotypes O157, O26 and O111) and Salmonella spp. (teagasc.ie)
  • Lactococcus lactis QU 5 isolated from corn produces a novel bacteriocin, termed lacticin Q. By acetone precipitation, cation-exchange chromatography, and reverse-phase high-performance liquid chromatography, lacticin Q was purified from the culture supernatant of this organism, and its molecular mass was determined to be 5,926.50 Da by mass spectrometry. (mysciencework.com)
  • Phylogenetic analysis of the concatenated sequences showed that X. gardneri, X. vesicatoria and X. perforans were associated with BST in Ethiopia, whereas Xanthomonas euvesicatoria was absent from the Ethiopian sample. (springer.com)
  • The potential applications of bacteriocins from LAB in the food and health care sectors have attracted the strong interest of academia and the industry resulting in an impressive amount of published research on their production, purification, genetics and applications. (biomedcentral.com)
  • The results described here clarified the fact that lacticin Q belongs to a new family of class II bacteriocins and that it can be employed as an alternative to or in combination with nisin A. (mysciencework.com)
  • Nisin is the most characterized of the class I bacteriocins. (hindawi.com)
  • 6,7 Notably, ASM1, a close homologue of GccF, and enterocin 96 are the only glycosylated bacteriocins which have been shown to be "glycoactive", 11-13 that is, the saccharide moieties are essential for biological activity. (rsc.org)
  • Enterocin K1 (EntK1), enterocin EJ97 (EntEJ97), and LsbB are three sequence related leaderless bacteriocins. (uio.no)
  • Enterocin S37 has a molecular weight comprised between 4 and 5 kDa. (hindawi.com)
  • Lacticin 3147, another bacteriocin produced by L. lactis , was tested for use in a teat sealant and reduced the number of S. aureus organisms recovered from the bovine mammary gland 18 h postchallenge compared to infusion with teat sealant alone ( 12 ). (asm.org)