The finer blood vessels of the vasculature that are generally less than 100 microns in internal diameter.
The circulation of the BLOOD through the MICROVASCULAR NETWORK.
The minute vessels that connect the arterioles and venules.
The minute vessels that collect blood from the capillary plexuses and join together to form veins.
The smallest divisions of the arteries located between the muscular arteries and the capillaries.
A layer of the peritoneum which attaches the abdominal viscera to the ABDOMINAL WALL and conveys their blood vessels and nerves.
Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.
The property of blood capillary ENDOTHELIUM that allows for the selective exchange of substances between the blood and surrounding tissues and through membranous barriers such as the BLOOD-AIR BARRIER; BLOOD-AQUEOUS BARRIER; BLOOD-BRAIN BARRIER; BLOOD-NERVE BARRIER; BLOOD-RETINAL BARRIER; and BLOOD-TESTIS BARRIER. Small lipid-soluble molecules such as carbon dioxide and oxygen move freely by diffusion. Water and water-soluble molecules cannot pass through the endothelial walls and are dependent on microscopic pores. These pores show narrow areas (TIGHT JUNCTIONS) which may limit large molecule movement.
A pathologic process consisting of the proliferation of blood vessels in abnormal tissues or in abnormal positions.
Specialized non-fenestrated tightly-joined ENDOTHELIAL CELLS with TIGHT JUNCTIONS that form a transport barrier for certain substances between the cerebral capillaries and the BRAIN tissue.
The circulation of blood through the BLOOD VESSELS supplying the abdominal VISCERA.
Unique slender cells with multiple processes extending along the capillary vessel axis and encircling the vascular wall, also called mural cells. Pericytes are imbedded in the BASEMENT MEMBRANE shared with the ENDOTHELIAL CELLS of the vessel. Pericytes are important in maintaining vessel integrity, angiogenesis, and vascular remodeling.
Any of the tubular vessels conveying the blood (arteries, arterioles, capillaries, venules, and veins).
The circulation of blood through the BLOOD VESSELS of the BRAIN.
The carbohydrate-rich zone on the cell surface. This zone can be visualized by a variety of stains as well as by its affinity for lectins. Although most of the carbohydrate is attached to intrinsic plasma membrane molecules, the glycocalyx usually also contains both glycoproteins and proteoglycans that have been secreted into the extracellular space and then adsorbed onto the cell surface. (Alberts et al., Molecular Biology of the Cell, 3d ed, p502)
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
The veins and arteries of the HEART.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
The blood vessels which supply and drain the RETINA.
The physiological widening of BLOOD VESSELS by relaxing the underlying VASCULAR SMOOTH MUSCLE.
The development of new BLOOD VESSELS during the restoration of BLOOD CIRCULATION during the healing process.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.
The innermost layer of the three meninges covering the brain and spinal cord. It is the fine vascular membrane that lies under the ARACHNOID and the DURA MATER.
The original member of the family of endothelial cell growth factors referred to as VASCULAR ENDOTHELIAL GROWTH FACTORS. Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as "tumor angiogenesis factor" and "vascular permeability factor". Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating VASODILATION via NITRIC OXIDE-dependent pathways. Alternative splicing of the mRNA for vascular endothelial growth factor A results in several isoforms of the protein being produced.
Cell adhesion molecules present on virtually all monocytes, platelets, and granulocytes. CD31 is highly expressed on endothelial cells and concentrated at the junctions between them.
A technique to study CELL MIGRATION in the INFLAMMATION process or during immune reactions. After an area on the skin is abraded, the movement of cells in the area is followed via microscopic observation of the exudate through a coverslip or tissue culture chamber placed over the area.
Microscopy in which television cameras are used to brighten magnified images that are otherwise too dark to be seen with the naked eye. It is used frequently in TELEPATHOLOGY.
A free radical gas produced endogenously by a variety of mammalian cells, synthesized from ARGININE by NITRIC OXIDE SYNTHASE. Nitric oxide is one of the ENDOTHELIUM-DEPENDENT RELAXING FACTORS released by the vascular endothelium and mediates VASODILATION. It also inhibits platelet aggregation, induces disaggregation of aggregated platelets, and inhibits platelet adhesion to the vascular endothelium. Nitric oxide activates cytosolic GUANYLATE CYCLASE and thus elevates intracellular levels of CYCLIC GMP.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Nutrient blood vessels which supply the walls of large arteries or veins.
The physiological narrowing of BLOOD VESSELS by contraction of the VASCULAR SMOOTH MUSCLE.
Movement of tethered, spherical LEUKOCYTES along the endothelial surface of the microvasculature. The tethering and rolling involves interaction with SELECTINS and other adhesion molecules in both the ENDOTHELIUM and leukocyte. The rolling leukocyte then becomes activated by CHEMOKINES, flattens out, and firmly adheres to the endothelial surface in preparation for transmigration through the interendothelial cell junction. (From Abbas, Cellular and Molecular Immunology, 3rd ed)
A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.
A nonapeptide messenger that is enzymatically produced from KALLIDIN in the blood where it is a potent but short-lived agent of arteriolar dilation and increased capillary permeability. Bradykinin is also released from MAST CELLS during asthma attacks, from gut walls as a gastrointestinal vasodilator, from damaged tissues as a pain signal, and may be a neurotransmitter.
White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).
Veins which return blood from the intestines; the inferior mesenteric vein empties into the splenic vein, the superior mesenteric vein joins the splenic vein to form the portal vein.
Drugs used to cause dilation of the blood vessels.
The thin layer of GRAY MATTER on the surface of the CEREBRAL HEMISPHERES that develops from the TELENCEPHALON and folds into gyri and sulchi. It reaches its highest development in humans and is responsible for intellectual faculties and higher mental functions.
The circulation of blood through the CORONARY VESSELS of the HEART.
A value equal to the total volume flow divided by the cross-sectional area of the vascular bed.
A species of the family Ranidae occurring in a wide variety of habitats from within the Arctic Circle to South Africa, Australia, etc.
The part of the face that is below the eye and to the side of the nose and mouth.
A system of organs and tissues that process and transport immune cells and LYMPH.
A villous structure of tangled masses of BLOOD VESSELS contained within the third, lateral, and fourth ventricles of the BRAIN. It regulates part of the production and composition of CEREBROSPINAL FLUID.
These growth factors are soluble mitogens secreted by a variety of organs. The factors are a mixture of two single chain polypeptides which have affinity to heparin. Their molecular weight are organ and species dependent. They have mitogenic and chemotactic effects and can stimulate endothelial cells to grow and synthesize DNA. The factors are related to both the basic and acidic FIBROBLAST GROWTH FACTORS but have different amino acid sequences.
A high-molecular-weight plasma protein, produced by endothelial cells and megakaryocytes, that is part of the factor VIII/von Willebrand factor complex. The von Willebrand factor has receptors for collagen, platelets, and ristocetin activity as well as the immunologically distinct antigenic determinants. It functions in adhesion of platelets to collagen and hemostatic plug formation. The prolonged bleeding time in VON WILLEBRAND DISEASES is due to the deficiency of this factor.
A family of angiogenic proteins that are closely-related to VASCULAR ENDOTHELIAL GROWTH FACTOR A. They play an important role in the growth and differentiation of vascular as well as lymphatic endothelial cells.
A CALCIUM-dependent, constitutively-expressed form of nitric oxide synthase found primarily in ENDOTHELIAL CELLS.
Adherence of cells to surfaces or to other cells.
Cell adhesion molecule and CD antigen that mediates the adhesion of neutrophils and monocytes to activated platelets and endothelial cells.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
Soluble protein factors generated by activated lymphocytes that affect other cells, primarily those involved in cellular immunity.
A layer of epithelium that lines the heart, blood vessels (ENDOTHELIUM, VASCULAR), lymph vessels (ENDOTHELIUM, LYMPHATIC), and the serous cavities of the body.
Precursors in the biosynthesis of prostaglandins and thromboxanes from arachidonic acid. They are physiologically active compounds, having effect on vascular and airway smooth muscles, platelet aggregation, etc.
An inhibitor of nitric oxide synthetase which has been shown to prevent glutamate toxicity. Nitroarginine has been experimentally tested for its ability to prevent ammonia toxicity and ammonia-induced alterations in brain energy and ammonia metabolites. (Neurochem Res 1995:200(4):451-6)
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Treatment process involving the injection of fluid into an organ or tissue.
Elements of limited time intervals, contributing to particular results or situations.
The arterial blood vessels supplying the CEREBRUM.
Cell-cell junctions that seal adjacent epithelial cells together, preventing the passage of most dissolved molecules from one side of the epithelial sheet to the other. (Alberts et al., Molecular Biology of the Cell, 2nd ed, p22)
An NADPH-dependent enzyme that catalyzes the conversion of L-ARGININE and OXYGEN to produce CITRULLINE and NITRIC OXIDE.
The nonstriated involuntary muscle tissue of blood vessels.
Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
A long flat muscle that extends along the whole length of both sides of the abdomen. It flexes the vertebral column, particularly the lumbar portion; it also tenses the anterior abdominal wall and assists in compressing the abdominal contents. It is frequently the site of hematomas. In reconstructive surgery it is often used for the creation of myocutaneous flaps. (From Gray's Anatomy, 30th American ed, p491)
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The vessels carrying blood away from the heart.
A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.
A ubiquitously expressed glucose transporter that is important for constitutive, basal GLUCOSE transport. It is predominately expressed in ENDOTHELIAL CELLS and ERYTHROCYTES at the BLOOD-BRAIN BARRIER and is responsible for GLUCOSE entry into the BRAIN.
Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.
Transmembrane proteins consisting of a lectin-like domain, an epidermal growth factor-like domain, and a variable number of domains that are homologous to complement regulatory proteins. They are important cell adhesion molecules which help LEUKOCYTES attach to VASCULAR ENDOTHELIUM.
The circulation of the BLOOD through the LUNGS.
A tissue preparation technique that involves the injecting of plastic (acrylates) into blood vessels or other hollow viscera and treating the tissue with a caustic substance. This results in a negative copy or a solid replica of the enclosed space of the tissue that is ready for viewing under a scanning electron microscope.
The flow of BLOOD through or around an organ or region of the body.
A colorimetric reagent for iron, manganese, titanium, molybdenum, and complexes of zirconium. (From Merck Index, 11th ed)
A neurotransmitter found at neuromuscular junctions, autonomic ganglia, parasympathetic effector junctions, a subset of sympathetic effector junctions, and at many sites in the central nervous system.
Localized reduction of blood flow to brain tissue due to arterial obstruction or systemic hypoperfusion. This frequently occurs in conjunction with brain hypoxia (HYPOXIA, BRAIN). Prolonged ischemia is associated with BRAIN INFARCTION.
Water-soluble proteins found in egg whites, blood, lymph, and other tissues and fluids. They coagulate upon heating.
A powerful vasodilator used in emergencies to lower blood pressure or to improve cardiac function. It is also an indicator for free sulfhydryl groups in proteins.
The force that opposes the flow of BLOOD through a vascular bed. It is equal to the difference in BLOOD PRESSURE across the vascular bed divided by the CARDIAC OUTPUT.
A claudin subtype that is found localized to TIGHT JUNCTIONS in VASCULAR ENDOTHELIAL CELLS. The protein was initially identified as one of several proteins which are deleted in VELOCARDIOFACIAL SYNDROME and may play an important role in maintaining the integrity of the BLOOD-BRAIN BARRIER.
A highly variable species of the family Ranidae in Canada, the United States and Central America. It is the most widely used Anuran in biomedical research.
An intermediate in the biosynthesis of cerebrosides. It is formed by reaction of sphingosine with UDP-galactose and then itself reacts with fatty acid-Coenzyme A to form the cerebroside.
Small encapsulated gas bubbles (diameters of micrometers) that can be used as CONTRAST MEDIA, and in other diagnostic and therapeutic applications. Upon exposure to sufficiently intense ultrasound, microbubbles will cavitate, rupture, disappear, release gas content. Such characteristics of the microbubbles can be used to enhance diagnostic tests, dissolve blood clots, and deliver drugs or genes for therapy.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
The internal resistance of the BLOOD to shear forces. The in vitro measure of whole blood viscosity is of limited clinical utility because it bears little relationship to the actual viscosity within the circulation, but an increase in the viscosity of circulating blood can contribute to morbidity in patients suffering from disorders such as SICKLE CELL ANEMIA and POLYCYTHEMIA.
A nucleoside that is composed of ADENINE and D-RIBOSE. Adenosine or adenosine derivatives play many important biological roles in addition to being components of DNA and RNA. Adenosine itself is a neurotransmitter.
Proteins that take part in the formation or structure of TIGHT JUNCTIONS.
A genus of the family Muridae having three species. The present domesticated strains were developed from individuals brought from Syria. They are widely used in biomedical research.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Tissue in the BASAL FOREBRAIN inferior to the anterior perforated substance, and anterior to the GLOBUS PALLIDUS and ansa lenticularis. It contains the BASAL NUCLEUS OF MEYNERT.
The deformation and flow behavior of BLOOD and its elements i.e., PLASMA; ERYTHROCYTES; WHITE BLOOD CELLS; and BLOOD PLATELETS.
A group of glucose polymers made by certain bacteria. Dextrans are used therapeutically as plasma volume expanders and anticoagulants. They are also commonly used in biological experimentation and in industry for a wide variety of purposes.
A group of physiologically active prostaglandin endoperoxides. They are precursors in the biosynthesis of prostaglandins and thromboxanes. The most frequently encountered member of this group is the prostaglandin H2.
Abnormal enlargement or swelling of a KIDNEY due to dilation of the KIDNEY CALICES and the KIDNEY PELVIS. It is often associated with obstruction of the URETER or chronic kidney diseases that prevents normal drainage of urine into the URINARY BLADDER.
Drugs used to cause constriction of the blood vessels.
Cell adhesion molecule and CD antigen that mediates neutrophil, monocyte, and memory T-cell adhesion to cytokine-activated endothelial cells. E-selectin recognizes sialylated carbohydrate groups related to the Lewis X or Lewis A family.
A strain of Rattus norvegicus used as a normotensive control for the spontaneous hypertensive rats (SHR).
The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
A 20-carbon-chain fatty acid, unsaturated at positions 8, 11, and 14. It differs from arachidonic acid, 5,8,11,14-eicosatetraenoic acid, only at position 5.
The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES.
A naturally occurring lipid pigment with histochemical characteristics similar to lipofuscin. It accumulates in various tissues in certain experimental and pathological conditions.
Diabetes mellitus induced experimentally by administration of various diabetogenic agents or by PANCREATECTOMY.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Small uniformly-sized spherical particles, of micrometer dimensions, frequently labeled with radioisotopes or various reagents acting as tags or markers.
Eicosatetraenoic acids substituted in any position by one or more hydroxy groups. They are important intermediates in a series of biosynthetic processes leading from arachidonic acid to a number of biologically active compounds such as prostaglandins, thromboxanes, and leukotrienes.
Agents and endogenous substances that antagonize or inhibit the development of new blood vessels.
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
A condition characterized by somnolence or coma in the presence of an acute infection with PLASMODIUM FALCIPARUM (and rarely other Plasmodium species). Initial clinical manifestations include HEADACHES; SEIZURES; and alterations of mentation followed by a rapid progression to COMA. Pathologic features include cerebral capillaries filled with parasitized erythrocytes and multiple small foci of cortical and subcortical necrosis. (From Adams et al., Principles of Neurology, 6th ed, p136)
An azo dye used in blood volume and cardiac output measurement by the dye dilution method. It is very soluble, strongly bound to plasma albumin, and disappears very slowly.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
The movement and the forces involved in the movement of the blood through the CARDIOVASCULAR SYSTEM.
Endogenously-synthesized compounds that influence biological processes not otherwise classified under ENZYMES; HORMONES or HORMONE ANTAGONISTS.
The circulation of the BLOOD through the vessels of the KIDNEY.
Emission or propagation of acoustic waves (SOUND), ELECTROMAGNETIC ENERGY waves (such as LIGHT; RADIO WAVES; GAMMA RAYS; or X-RAYS), or a stream of subatomic particles (such as ELECTRONS; NEUTRONS; PROTONS; or ALPHA PARTICLES).
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Disease of the RETINA as a complication of DIABETES MELLITUS. It is characterized by the progressive microvascular complications, such as ANEURYSM, interretinal EDEMA, and intraocular PATHOLOGIC NEOVASCULARIZATION.
An octapeptide that is a potent but labile vasoconstrictor. It is produced from angiotensin I after the removal of two amino acids at the C-terminal by ANGIOTENSIN CONVERTING ENZYME. The amino acid in position 5 varies in different species. To block VASOCONSTRICTION and HYPERTENSION effect of angiotensin II, patients are often treated with ACE INHIBITORS or with ANGIOTENSIN II TYPE 1 RECEPTOR BLOCKERS.
A MARVEL domain protein that plays an important role in the formation and regulation of the TIGHT JUNCTION paracellular permeability barrier.
A genus of the subfamily CERCOPITHECINAE, family CERCOPITHECIDAE, consisting of five named species: PAPIO URSINUS (chacma baboon), PAPIO CYNOCEPHALUS (yellow baboon), PAPIO PAPIO (western baboon), PAPIO ANUBIS (or olive baboon), and PAPIO HAMADRYAS (hamadryas baboon). Members of the Papio genus inhabit open woodland, savannahs, grassland, and rocky hill country. Some authors consider MANDRILLUS a subgenus of Papio.
An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.
The vascular resistance to the flow of BLOOD through the CAPILLARIES portions of the peripheral vascular bed.
A purely physical condition which exists within any material because of strain or deformation by external forces or by non-uniform thermal expansion; expressed quantitatively in units of force per unit area.
The pressure due to the weight of fluid.
A phospholipid derivative formed by PLATELETS; BASOPHILS; NEUTROPHILS; MONOCYTES; and MACROPHAGES. It is a potent platelet aggregating agent and inducer of systemic anaphylactic symptoms, including HYPOTENSION; THROMBOCYTOPENIA; NEUTROPENIA; and BRONCHOCONSTRICTION.
PRESSURE of the BLOOD on the ARTERIES and other BLOOD VESSELS.
A 21-amino acid peptide produced in a variety of tissues including endothelial and vascular smooth-muscle cells, neurons and astrocytes in the central nervous system, and endometrial cells. It acts as a modulator of vasomotor tone, cell proliferation, and hormone production. (N Eng J Med 1995;333(6):356-63)
Refers to animals in the period of time just after birth.
A strain of Rattus norvegicus with elevated blood pressure used as a model for studying hypertension and stroke.
The vessels carrying blood away from the capillary beds.
Cytokine-induced cell adhesion molecule present on activated endothelial cells, tissue macrophages, dendritic cells, bone marrow fibroblasts, myoblasts, and myotubes. It is important for the recruitment of leukocytes to sites of inflammation. (From Pigott & Power, The Adhesion Molecule FactsBook, 1993, p154)
A non-steroidal anti-inflammatory agent (NSAID) that inhibits the enzyme cyclooxygenase necessary for the formation of prostaglandins and other autacoids. It also inhibits the motility of polymorphonuclear leukocytes.
The main trunk of the systemic arteries.
A phthalic indicator dye that appears yellow-green in normal tear film and bright green in a more alkaline medium such as the aqueous humor.
Reduction of blood viscosity usually by the addition of cell free solutions. Used clinically (1) in states of impaired microcirculation, (2) for replacement of intraoperative blood loss without homologous blood transfusion, and (3) in cardiopulmonary bypass and hypothermia.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
A class of large neuroglial (macroglial) cells in the central nervous system - the largest and most numerous neuroglial cells in the brain and spinal cord. Astrocytes (from "star" cells) are irregularly shaped with many long processes, including those with "end feet" which form the glial (limiting) membrane and directly and indirectly contribute to the BLOOD-BRAIN BARRIER. They regulate the extracellular ionic and chemical environment, and "reactive astrocytes" (along with MICROGLIA) respond to injury.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Arteries which arise from the abdominal aorta and distribute to most of the intestines.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.
Porphyrins with four methyl, two ethyl, and two propionic acid side chains attached to the pyrrole rings.
An angiopoietin that is closely related to ANGIOPOIETIN-1. It binds to the TIE-2 RECEPTOR without receptor stimulation and antagonizes the effect of ANGIOPOIETIN-1. However its antagonistic effect may be limited to cell receptors that occur within the vasculature. Angiopoietin-2 may therefore play a role in down-regulation of BLOOD VESSEL branching and sprouting.
A cell-surface ligand involved in leukocyte adhesion and inflammation. Its production is induced by gamma-interferon and it is required for neutrophil migration into inflamed tissue.
A TIE receptor tyrosine kinase that is found almost exclusively on ENDOTHELIAL CELLS. It is required for both normal embryonic vascular development (NEOVASCULARIZATION, PHYSIOLOGIC) and tumor angiogenesis (NEOVASCULARIZATION, PATHOLOGIC).
A large group of membrane transport proteins that shuttle MONOSACCHARIDES across CELL MEMBRANES.
A complex of polyene antibiotics obtained from Streptomyces filipinensis. Filipin III alters membrane function by interfering with membrane sterols, inhibits mitochondrial respiration, and is proposed as an antifungal agent. Filipins I, II, and IV are less important.
A non-selective inhibitor of nitric oxide synthase. It has been used experimentally to induce hypertension.
The volume of packed RED BLOOD CELLS in a blood specimen. The volume is measured by centrifugation in a tube with graduated markings, or with automated blood cell counters. It is an indicator of erythrocyte status in disease. For example, ANEMIA shows a low value; POLYCYTHEMIA, a high value.
A 200-230-kDa tyrosine kinase receptor for vascular endothelial growth factors found primarily in endothelial and hematopoietic cells and their precursors. VEGFR-2 is important for vascular and hematopoietic development, and mediates almost all endothelial cell responses to VEGF.
Theoretical representations that simulate the behavior or activity of the cardiovascular system, processes, or phenomena; includes the use of mathematical equations, computers and other electronic equipment.
Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis.
Conjugated proteins in which mucopolysaccharides are combined with proteins. The mucopolysaccharide moiety is the predominant group with the protein making up only a small percentage of the total weight.
Inosine 5'-(tetrahydrogen triphosphate). An inosine nucleotide containing three phosphate groups esterified to the sugar moiety. Synonym: IRPPP.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Interruption of sympathetic pathways, by local injection of an anesthetic agent, at any of four levels: peripheral nerve block, sympathetic ganglion block, extradural block, and subarachnoid block.
Genetically developed small pigs for use in biomedical research. There are several strains - Yucatan miniature, Sinclair miniature, and Minnesota miniature.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
An enzyme that catalyzes the oxidative deamination of naturally occurring monoamines. It is a flavin-containing enzyme that is localized in mitochondrial membranes, whether in nerve terminals, the liver, or other organs. Monoamine oxidase is important in regulating the metabolic degradation of catecholamines and serotonin in neural or target tissues. Hepatic monoamine oxidase has a crucial defensive role in inactivating circulating monoamines or those, such as tyramine, that originate in the gut and are absorbed into the portal circulation. (From Goodman and Gilman's, The Pharmacological Basis of Therapeutics, 8th ed, p415) EC 1.4.3.4.
(Z)-9-Octadecenoic acid 1,2,3-propanetriyl ester.
The neural systems which act on VASCULAR SMOOTH MUSCLE to control blood vessel diameter. The major neural control is through the sympathetic nervous system.
Relatively complete absence of oxygen in one or more tissues.
Compounds or agents that combine with cyclooxygenase (PROSTAGLANDIN-ENDOPEROXIDE SYNTHASES) and thereby prevent its substrate-enzyme combination with arachidonic acid and the formation of eicosanoids, prostaglandins, and thromboxanes.
Aquaporin 4 is the major water-selective channel in the CENTRAL NERVOUS SYSTEM of mammals.
A branch of the abdominal aorta which supplies the kidneys, adrenal glands and ureters.
Measurement of oxygen and carbon dioxide in the blood.
A hypoperfusion of the BLOOD through an organ or tissue caused by a PATHOLOGIC CONSTRICTION or obstruction of its BLOOD VESSELS, or an absence of BLOOD CIRCULATION.
A type of stress exerted uniformly in all directions. Its measure is the force exerted per unit area. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A subtype of striated muscle, attached by TENDONS to the SKELETON. Skeletal muscles are innervated and their movement can be consciously controlled. They are also called voluntary muscles.
The first to be discovered member of the angiopoietin family. It may play a role in increasing the sprouting and branching of BLOOD VESSELS. Angiopoietin-1 specifically binds to and stimulates the TIE-2 RECEPTOR. Several isoforms of angiopoietin-1 occur due to ALTERNATIVE SPLICING of its mRNA.
A single-chain polypeptide growth factor that plays a significant role in the process of WOUND HEALING and is a potent inducer of PHYSIOLOGIC ANGIOGENESIS. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FIBROBLAST GROWTH FACTOR 1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor (FIBROBLAST GROWTH FACTOR 1).
A generic term used to describe a group of polypeptides with related chemical structures and pharmacological properties that are widely distributed in nature. These peptides are AUTACOIDS that act locally to produce pain, vasodilatation, increased vascular permeability, and the synthesis of prostaglandins. Thus, they comprise a subset of the large number of mediators that contribute to the inflammatory response. (From Goodman and Gilman's The Pharmacologic Basis of Therapeutics, 8th ed, p588)
A statistical technique that isolates and assesses the contributions of categorical independent variables to variation in the mean of a continuous dependent variable.
A potent oxidant synthesized by the cell during its normal metabolism. Peroxynitrite is formed from the reaction of two free radicals, NITRIC OXIDE and the superoxide anion (SUPEROXIDES).
A diverse group of agents, with unique chemical structures and biochemical requirements, which generate NITRIC OXIDE. These compounds have been used in the treatment of cardiovascular diseases and the management of acute myocardial infarction, acute and chronic congestive heart failure, and surgical control of blood pressure. (Adv Pharmacol 1995;34:361-81)
Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.
Formation or presence of a blood clot (THROMBUS) in a blood vessel within the SKULL. Intracranial thrombosis can lead to thrombotic occlusions and BRAIN INFARCTION. The majority of the thrombotic occlusions are associated with ATHEROSCLEROSIS.
A darkly stained mat-like EXTRACELLULAR MATRIX (ECM) that separates cell layers, such as EPITHELIUM from ENDOTHELIUM or a layer of CONNECTIVE TISSUE. The ECM layer that supports an overlying EPITHELIUM or ENDOTHELIUM is called basal lamina. Basement membrane (BM) can be formed by the fusion of either two adjacent basal laminae or a basal lamina with an adjacent reticular lamina of connective tissue. BM, composed mainly of TYPE IV COLLAGEN; glycoprotein LAMININ; and PROTEOGLYCAN, provides barriers as well as channels between interacting cell layers.
An antidiabetic sulfonylurea derivative with actions similar to those of chlorpropamide.
A technique of inputting two-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer.
Physiologically active prostaglandins found in many tissues and organs. They show pressor activity, are mediators of inflammation, and have potential antithrombotic effects.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Salts and esters of hippuric acid.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
A biocompatible polymer used as a surgical suture material.
Devices for accelerating protons or electrons in closed orbits where the accelerating voltage and magnetic field strength varies (the accelerating voltage is held constant for electrons) in order to keep the orbit radius constant.
An enzyme that catalyzes the transfer of 2-deoxy-D-ribose from THYMIDINE to orthophosphate, thereby liberating thymidine.
Adverse functional, metabolic, or structural changes in ischemic tissues resulting from the restoration of blood flow to the tissue (REPERFUSION), including swelling; HEMORRHAGE; NECROSIS; and damage from FREE RADICALS. The most common instance is MYOCARDIAL REPERFUSION INJURY.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
A method of non-invasive, continuous measurement of MICROCIRCULATION. The technique is based on the values of the DOPPLER EFFECT of low-power laser light scattered randomly by static structures and moving tissue particulates.
Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.
Abnormal fluid accumulation in TISSUES or body cavities. Most cases of edema are present under the SKIN in SUBCUTANEOUS TISSUE.
Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.

Large and micro coronary vascular involvement in diabetes. (1/1245)

Diabetes, with hyperglycemia as its hallmark, is a major risk factor for ischemic heart disease. The role of coronary disease in the adverse prognosis of diabetes is controversial although the higher prevalence and extension of coronary atherosclerosis is well recognized. The paper reviews the available evidence of coronary involvement in diabetes with particular emphases on microcirculation. Several studies, mainly in type 2 diabetes, have documented a reduced coronary flow reserve even in absence of coronary obstructive disease and using different techniques. Microcirculatory dysfunction affects the left ventricle globally as well as regionally. However, neither the prevalence of such abnormality in the diabetic population nor its time course and its prognostic value have been investigated in specifically addressed studies. In fact, a relatively large number of studies on myocardial perfusion performed by single-photon myocardial scintigraphy in asymptomatic diabetics rather address the problem of the prevalence of silent ischemia and its prognostic value. In spite of such limitation it can be speculated from the few available studies with known coronary anatomy that the prevalence of exclusively regional disturbances of perfusion (scintigraphic defects) in absence of obstructive coronary disease is not marginal as it ranges from 11 to 63%. Extensive research is still required to define the pathogenesis and the actual clinical relevance of coronary microcirculatory dysfunction in diabetes.  (+info)

Purinoceptors in renal microvessels: adenosine-activated and cytochrome P450 monooxygenase-derived arachidonate metabolites. (2/1245)

Cytochrome P450 (CYP)-dependent epoxyeicosatrienoic acids (EETs) dilate rat preglomerular microvessels (PGMVs) when adenosine 2A receptors (A(2A)R) are stimulated. As high salt intake increases epoxygenase activity and adenosine levels, we hypothesized that renal adenosine responses would be greater in high salt-fed rats. We have obtained evidence supporting this hypothesis in rats fed a high salt diet for 7 days. Stimulation of adenosine receptors with 2-chloroadenosine in kidneys obtained from rats on high salt (4%) intake produced an increase in EET release that was several-fold greater than in kidneys of rats on normal salt (0.4% NaCl) diets, which was associated with a sharp decline in renovascular resistance. Under conditions of high salt intake, an associated upregulation of A(2A)R and 2C23 protein expression was observed. As EETs are renal vasodilator and natriuretic eicosanoids, the antipressor response to salt loading may operate through an A(2A)R - EET mechanism. These findings expand the role of adenosine-related mechanisms in protecting renal function.  (+info)

Inflammatory pathways and microvascular responses in the lung. (3/1245)

Neutrophil granulocytes constitute an important host defense mechanism, but may at the same time damage functional tissue and propagate acute organ failure. This balance is particularly vulnerable in the lung which provides a large surface area for invading pathogens and microorganisms, and simultaneously harbors a large pool of physiologically marginated neutrophils within its microvascular bed. Pathophysiological stimuli further amplify this accumulation of blood cells and promote the emigration of neutrophils into the pulmonary interstitium and the airspaces by different mechanisms depending on the pathophysiological stimulus, its route of entry into or site of production in the lung, and the time course of its action. Importantly, the pulmonary microvascular endothelium plays a key role in regulating not only sequestration and emigration of neutrophils, but by initiating the inflammatory response to a variety of diverse stimuli many of which do not directly target the circulating neutrophil, but elicit microvascular reactions by primarily acting on the endothelium. This review highlights the inflammatory process in the pulmonary microvasculature with special emphasis on the role of the pulmonary endothelium.  (+info)

Effects of basic fibroblast growth factor on the expressions of angiogenic gene profile and cyclooxygenase-2 in brain microvascular endothelial cells. (4/1245)

The present study aimed to investigate the effects of basic fibroblast growth factor (bFGF) on the expressions of angioge-nesis-related genes in a mouse brain microvascular endothelial cell line, namely bEnd.3, using cDNA microarray. The effects of bFGF (10 ng/ml) on mRNA and protein expressions of cyclooxygenase-2 (COX-2), an angiogenesis bystander molecule, were further investigated. cDNA microarray was employed to study the effects of bFGF on the expressions of angiogenic genes in a high throughput pattern. RT-PCR was used to study the effect of bFGF on COX-2 mRNA expression. Western blot and immunocytochemistry were utilized to study the effect of bFGF on COX-2 protein expression. The results showed that, 2 h after bFGF treatment, pro-angiogenic genes (Adamts1, MMP-9, Ang-1, PDGF B, G-CSF, FGF16, IGF-1, etc.) were significantly upregulated, whereas anti-angiogenic genes (TIMP-2, TSP-3, etc.) were significantly downregulated. The bystander molecule in angiogenic pathway COX-2 mRNA and protein expressions were significantly upregulated after bFGF treatment. It is suggested that triggering angiogensis switch through upregulating pro-angiogenic gene and downregulating anti-angiogenic gene expression is one of the major mechanisms of bFGF-induced angiogenesis. The expression change of COX-2, as a bystander molecule, was observed after bFGF treatment in bEnd.3 cells and the significance was discussed.  (+info)

Microvascular engineering in perfusion culture: immunohistochemistry and CLSM findings. (5/1245)

BACKGROUND: One of the most challenging problems in tissue engineering is the establishment of vascular supply. A possible approach might be the engineering of microvasculature in vitro and the supply by engineered feeder vessels. METHODS: An in vitro model for a small-diameter vessel was developed and made from adipose tissue stromal cells and human umbilical vein endothelial cells in a tube-like gelatine scaffold. The number of "branches" emerging from the central lumen and the morphology of the central lumen of the vessel equivalent were assessed after 16 days of either pulsatile perfusion culture or culture in rotating containers by evaluation of immunohistochemically stained sections (n = 6 pairs of cultures). Intramural capillary network formation was demonstrated in five experiments with confocal laser scanning microscopy. RESULTS: Perfused specimens showed a round or oval lumen lined by a single layer of endothelial cells, whereas following rotation culture the lumen tended to collapse. Confocal laser scanning microscopy showed more extended network formation in perfused specimens as compared to specimens after rotation culture. Partially highly interconnected capillary-like networks were imaged which showed orientation around the central lumen. Perfused specimens exhibited significantly more branches emerging from the central lumen. There were, however, hardly any capillary branches crossing the whole vessel wall. CONCLUSION: Pulsatile perfusion supports the development of vascular networks with physiological appearance. Advances in reactor development, acquisition of functional data and imaging procedures are however necessary in order to attain the ultimate goal of a fully functional engineered supplying vessel.  (+info)

Gap junctions synchronize vascular tone within the microcirculation. (6/1245)

Gap junctions are formed in the cardiovascular system by connexin40 (Cx40), Cx37, Cx43, and Cx45. These low resistance channels allow the transfer of ions and small molecules between cells. The longitudinal coupling of endothelial and smooth muscle cells via gap junctions allows the spread of changes in membrane potential along the vascular wall and hence provides conduction pathways within the vessel itself. Functionally, this tight coupling is reflected by the spread of locally initiated vasomotor responses along the arteriole which are termed conducted responses. Conducted dilations are initiated by the application of endothelium-dependent stimuli which result in local hyperpolarization. This signal spreads along the wall, most likely along the endothelial cell layer, to elicit a coordinated dilation of the arteriole over a considerable distance. Likewise, the opposite signal (depolarization) spreads along the vessel giving rise to a conducted constriction. The latter response is however most likely transmitted along the smooth muscle cell layer. Thus, conducted responses reflect the synchronized behavior of the cells of the vascular wall. It is assumed that conducted responses are critical for the matching of oxygen delivery and tissue needs because they contribute to an ascending dilation which lowers resistance along the length of the arterioles and upstream vessels in a well-tuned fashion. Herein, Cx40 is of special importance because it is critically required for intact signal transduction along the endothelial cell layer. In addition, Cx40 mediates pressure feedback inhibition on renin synthesis in the kidney. Both, vascular and renal function of Cx40, may be involved in the hypertension that is observed in Cx40-deficient animals. In this review, we will summarize physiologic function of connexins in arterioles and briefly address their role in the kidney with respect to renin secretion.  (+info)

Tetracycline suppresses ATP gamma S-induced CXCL8 and CXCL1 production by the human dermal microvascular endothelial cell-1 (HMEC-1) cell line and primary human dermal microvascular endothelial cells. (7/1245)

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Comparing contrast-enhanced color flow imaging and pathological measures of breast lesion vascularity. (8/1245)

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This study examined the microcirculatory and renin-angiotensin system changes following the reversal of hypertension in reduced renal mass rats. Nine-week-old Sprague-Dawley reduced renal mass rats were placed on a low or high sodium diet for 4 or 8 weeks or a combination of 4 weeks of high sodium followed by 4 weeks of low sodium. Blood pressure was directly measured during the development of hypertension and its reversal. Plasma renin activity, angiotensin-converting enzyme activity, and angiotensin II concentrations were measured throughout the experiment. The cremaster and hindlimb muscles were removed, and microvascular density was determined by quantitative stereology. Four weeks of high sodium increased blood pressure (152+/-7 mm Hg) and reduced microvessel density (13.7%). Reduced renal mass hypertension was rapidly reversed after the rats were returned to a low sodium diet (124+/-7 mm Hg after 3 days), and microvascular density returned to control levels. After 4 weeks of high sodium, ...
Dmitry N. Grigoryev is the author of this article in the Journal of Visualized Experiments: RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells
Dette manuskriptet presenterer en injeksjon metode for å konstruere microvessels at rekapitulere fysiologiske egenskapene til...
Hamster Small Intestinal Microvascular Endothelial Cells from Creative Bioarray are isolated from small intestine of pathogen-free laboratory mice. Hamster Small Intestinal Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods ...
In this study, we demonstrate that curcumin exerts potent effects on the angiogenic properties of microvascular endothelial cells isolated from the human intestine, inhibiting multiple stages in the angiogenic process. We have demonstrated that (1) COX-2 induction as well as prostanoid production induced by VEGF were blocked by curcumin; (2) VEGF-induced growth, proliferation, transmigration and tube formation in HIMECs, an in vitro strategy for modelling angiogenesis, were also inhibited by curcumin; (3) the COX-2-specific inhibitor NS398, inhibited HIMEC growth, proliferation, transmigration and tube formation induced by VEGF; and (4) MAPK family members are involved in VEGF-induced upregulation of COX-2 and PGE2 production in HIMECs.. The cyclo-oxygenase enzymes COX-1 and COX-2 have been shown to play an important role in the regulation of angiogenesis.33 These enzymes catalyse the conversion of arachidonic acid to PGH2, the first step in the biosynthesis of the PGs thromboxane and ...
Author(s): Tran, Edward Duc | Abstract: Recent evidence suggests that endothelial apoptosis may be a mechanism for capillary rarefaction in hypertensives. The objective of this study is to examine the early phase of cell apoptosis and capillary blood flow in single capillaries of the rat mesentery in the spontaneously hypertensive rat (SHR) and its normotensive control, the Wistar-Kyoto (WKYs) rat. Since hypertension in the SHR is critically dependent on glucocorticoids, the animals were treated with a synthetic glucocorticoid, dexamethasone (DEX), by intraperitoneal injection and by superfusion. Capillaries with single file flow of cells in mesentery were observed in-vivo at high resolution during a period of that leads to permanent stasis without central pressure reduction. Propidium iodide (PI) was used as a marker to detect apoptosis. Continuous observation of apoptotic cells showed that permanent stasis in capillaries is initiated by the entrapment of leukocytes at the location of an endothelial
We demonstrated that, for whole tumors exclusive of any central cystic or necrotic core, total and microvascular blood volume are increased relative to contralateral brain, and mVD is enlarged. These findings are consistent with neoplastic angiogenesis that may follow an initial period of vascular cooption (40). For whole tumors, total blood volume and mVD are predictive of tumor grade, whereas the microvascular blood volume is not. However, when we considered tumor microvascular hot spots, the microvascular blood volume was predictive of tumor grade. This finding is consistent with those of previous imaging studies in which SE rCBV techniques alone were used (12, 21), and those in which the standard histologic approach of using the highest microvessel count to predict tumor angiogenesis and grade was applied (4, 41). In contrast, the ratio was only weakly correlated with grade when we considered only SE rCBV hot spots. This result is consistent with the idea that the GE-SE ratio is a marker of ...
Akt phosphorylation by GDF-15 in endothelial cells. GDF-15 induced Akt phosphorylation at Ser437 in human pulmonary microvascular endothelial cells (HPMEC). The
Objective: To explore the role and mechanism of mesenchymal stem cell (MSC) in modulating human pulmonary microvascular endothelial cell (HPMECs) permeability via hepatocyte growth factor (HGF). Methods: The study introduced a co-cultured model between HPMECs and human mesenchymal stem cell conditioned media (MSC-CM) collected from MSCs after 24 h hypoxia culture, and meanwhile HGF was neutralized in MSC-CM by anti-HGF antibody respectively, followed by lipopolysaccharide (LPS) stimulation. Finally, the following measurements were performed: the permeability of HPMECs, the protein expression of vascular endothelial cadherin (VE-cadherin), Occludin in HPMECs by Western blot, HPMECs apoptosis by Annexin V-FITC/PI and HPMECs proliferation by 3-(4, 5)-dimethylthiahiazo (-z-y1)-3, 5-di- phenytetrazoliumromide(MTT ...
Recapitulation of microvascular structure, function and perfusion in vitro can enable studies of vascular biology, provide a model for diseases such as ischemic stroke or tumor angiogenesis and enable quantitative evaluation of physiologic blood or lymph perfusion. Here we describe the initial design and deployment of a first-generation, self-contained 3D-printed, physiologically-faithful, microfluidic perfusion phantom to form explicit, hierarchically-branching, microvascular structure encapsulated in a type I collagen matrix in vitro, with pump-driven perfusion easily visible via phase-contrast MRI (Fig. 1). The phantom flexibly supports creation of user-defined vessel network geometries with human vascular cells and allows experimental validation of blood flow, i.e., via constitutive equations for convective and diffusive transport that quantitatively relate the flux of tracers from time-resolved images to transport field quantities. Thus, the largely qualitative and unmeasurable global ...
In this study, we investigated the mechanisms underlying the changes in intestinal microcirculatory oxygenation induced by acute normovolemic HD. Our results show that severe HD is associated with redistribution of O2 delivery away from the intestines and also shunting of the residual O2 within the intestines itself. The correlation between mucosal μHbO2 and μPo2 during reduction of intestinal microvascular oxygenation indicates that impaired erythrocyte O2 off-loading is not the key mechanism responsible for the decreased microcirculatory mucosal oxygenation. Thus O2 shunting bypassing the microcirculation to the venous compartment appears to be the main factor. Herein, rheological alterations may contribute, and a potential candidate is the observed erythrocyte rigidification during ANH.. In HD pigs, mucosal μHbO2 was well preserved during mild ANH but finally progressively declined. This finding of a two-phase response of intestinal microvascular oxygenation (maintained oxygenation during ...
The microvascular network is a simple but critical system that is responsible for various important biological mechanisms in the bodies of all animals. The ability to generate a functional microvessel in vitro not only makes it possible to engineer vital tissue of considerable size but also serves as a platform for biomedical studies. In this study, we propose a simple method for fabricating customized cylinder micro-scaffolds for the in vitro development of microvascular networks. By integrating micro-electro-mechanical systems techniques with thermal reflow, we design and fabricate a micro-scale hemi-cylinder photoresist template. Then, a replica mold of polydimethylsiloxane, produced by casting, is used to generate microvascular network scaffolds of poly(lactide-co-glycolide) (PLGA). We selected the human umbilical vein endothelial cell (HUVEC) as our model endothelial cell, seeded it onto both sides of the PLGA scaffold, and cultured it using a traditional approach with no pumping system. ...
There is growing evidence supporting the notion that endothelial dysfunction and inflammation are common events driving the development of various retinal microvascular changes in patients with hypertension, diabetes, and other metabolic disorders [30,31]. However, the synergistic impact of these commonly coexisting conditions on the development of retinopathy has not been fully elucidated. Our current study aimed to model the early and established detrimental effects of combining hypertension with diabetes on the development of retinopathy and retinal microvascular degeneration using diabetic spontaneously hypertensive rats, an established model for studying hypertension. Clinical evidence highlights the role of oxidative stress and endothelial dysfunction as previously indicated by increased levels of lipid peroxidation and platelet activation in patients with hypertension-related microvascular changes when compared with patients with or without early signs of retinopathy [3,30]. Our results ...
BACKGROUND: Acute kidney injury (AKI), even if followed by renal recovery, is a risk factor for the future development of chronic kidney disease (CKD) and end-stage renal disease (ESRD). In the previous years, novel insights in the pathophysiology of CKD progression suggested a causal link between AKI and CKD due to a maladaptive repair after severe and repeated injury. SUMMARY: Several pathological mechanisms have been proposed to contribute to the progression of AKI and transition to CKD/ESRD including hypoxia and microvascular rarefaction, alterations of renal resident cell phenotypes and functions, cell cycle arrest in the G2/M phase, persistent chronic inflammation, and development of interstitial fibrosis, mitochondrial fragmentation, epigenetic changes, activation of renin-angiotensin system (RAS), cell and tissue senescence ...
PELOBIOTECH GmbH offers a broad range of primary cells, stem cells, genetically modified cell systems and 3D cell models. To be able to offer you these premium products we work together with companies worldwide. This will guarantee best quality and a huge number of highly innovative products and services ...
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Purpose: To describe the prevalence of retinal microvascular characteristics and their associations with atherosclerosis in elderly, nondiabetic persons. Design and Participants: Population-based, cross-sectional study comprising 2050 men and women aged 69 to 97 years without diabetes, living in four communities. Methods: Participants underwent retinal photography and standardized grading of retinal microvascular characteristics, including retinopathy (e.g., microaneurysms, retinal hemorrhages), focal arteriolar narrowing, and arteriovenous nicking. In addition, calibers of retinal arterioles and venules were measured on digitized photographs to obtain an estimate of generalized arteriolar narrowing. Atherosclerosis and its risk factors were obtained from clinical examination and laboratory investigations. Main Outcome Measures: Prevalence of retinal microvascular abnormalities and their associations with measures of atherosclerosis. Results: The prevalence of retinal microvascular abnormalities ...
TY - JOUR. T1 - Microvascular pathology and morphometrics of sporadic and hereditary small vessel diseases of the brain. AU - Craggs, Lucinda J L. AU - Yamamoto, Yumi. AU - Deramecourt, Vincent. AU - Kalaria, Raj N.. N1 - No record of this in Eprints. HN 29/11/2017. PY - 2014/9. Y1 - 2014/9. N2 - Small vessel diseases (SVDs) of the brain are likely to become increasingly common in tandem with the rise in the aging population. In recent years, neuroimaging and pathological studies have informed on the pathogenesis of sporadic SVD and several single gene (monogenic) disorders predisposing to subcortical strokes and diffuse white matter disease. However, one of the limitations toward studying SVD lies in the lack of consistent assessment criteria and lesion burden for both clinical and pathological measures. Arteriolosclerosis and diffuse white matter changes are the hallmark features of both sporadic and hereditary SVDs. The pathogenesis of the arteriopathy is the key to understanding the ...
Previous studies suggest white matter (WM) integrity is vulnerable to chronic hypoperfusion during brain ageing. We assessed ~ 0.7 million capillary profiles in the frontal lobe WM across several dementias comprising Alzheimers disease, dementia with Lewy bodies, Parkinsons disease with dementia, vascular dementia, mixed dementias, post-stroke dementia as well as post-stroke no dementia and similar age ageing and young controls without significant brain pathology. Standard histopathological methods were used to determine microvascular pathology and capillary width and densities in 153 subjects using markers of the basement membrane (collagen IV; COL4) and endothelium (glucose transporter-1; GLUT-1). Variable microvascular pathology including coiled, tortuous, collapsed and degenerated capillaries as well as occasional microaneurysms was present in all dementias. As expected, WM microvascular densities were 20-49% lower than in the overlying cortex. This differential in density between WM and cortex
Previous studies suggest white matter (WM) integrity is vulnerable to chronic hypoperfusion during brain ageing. We assessed ~ 0.7 million capillary profiles in the frontal lobe WM across several dementias comprising Alzheimers disease, dementia with Lewy bodies, Parkinsons disease with dementia, vascular dementia, mixed dementias, post-stroke dementia as well as post-stroke no dementia and similar age ageing and young controls without significant brain pathology. Standard histopathological methods were used to determine microvascular pathology and capillary width and densities in 153 subjects using markers of the basement membrane (collagen IV; COL4) and endothelium (glucose transporter-1; GLUT-1). Variable microvascular pathology including coiled, tortuous, collapsed and degenerated capillaries as well as occasional microaneurysms was present in all dementias. As expected, WM microvascular densities were 20-49% lower than in the overlying cortex. This differential in density between WM and cortex
Title:Mimicking Microvascular Alterations of Human Diabetic Retinopathy: A Challenge for the Mouse Models. VOLUME: 20 ISSUE: 26. Author(s):D. Ramos, A. Carretero, M. Navarro, L. Mendes-Jorge, V. Nacher, A. Rodriguez-Baeza and J. Ruberte. Affiliation:Center for Animal Biotechnology and Gene Therapy (CBATEG), Universitat Autonoma de Barcelona, E-08193-Bellaterra, Spain.. Keywords:Diabetes, retinopathy, microvascular complication, mouse model.. Abstract:Although it has become acceptable that neuroretinal cells are also affected in diabetes, vascular lesions continue to be considered as the hallmarks of diabetic retinopathy. Animal models are essential for the understanding and treatment of human diabetic retinopathy, and the mouse is intensively used as a model because of its similarity to human and the possibility to be genetically modified. However, until today not all retinal vascular lesions developed in diabetic patients have been reproduced in diabetic mice, and the reasons for this are not ...
The immunosuppressive agent cyclosporin A (CsA), a calcineurin inhibitor which blocks T cell activation has provided the pharmacologic foundation for organ transplantation. CsA exerts additional effects on non-immune cell populations and may adversely effect microvascular endothelial cells, contributing to chronic rejection, a long-term clinical complication and significant cause of mortality in solid-organ transplants, including patients with small bowel allografts. Growth of new blood vessels, or angiogenesis, is a critical homeostatic mechanism in organs and tissues, and regulates vascular populations in response to physiologic requirements. We hypothesized that CsA would inhibit the angiogenic capacity of human gut microvessels. Primary cultures of human intestinal microvascular endothelial cells (HIMEC) were used to evaluate CsAs effect on four in vitro measures of angiogenesis, including endothelial stress fiber assembly, migration, proliferation and tube formation, in response to the endothelial
C57BL/6-GFP Mouse Skeletal Muscle Microvascular Endothelial Cells from Creative Bioarray are isolated from C57BL/6-Tg (CAG-EGFP) 1Osb/J mouse skeletal muscle tissue of pathogen-free laboratory mice. C57BL/6-GFP Mouse Skeletal Muscle Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic pre-coated with secondary antibody ...
The hippocampus is one of the earliest and most affected regions in Alzheimers disease (AD), followed by the cortex while the cerebellum is largely spared. Importantly, endothelial dysfunction is a common feature of cerebral blood vessels in AD. In this study, we sought to determine if regional heterogeneity of cerebral microvessels might help explain the susceptibility of the hippocampus and cortex as compared to the cerebellum. We isolated microvessels from wild type mice from the cerebellum, cortex, and hippocampus to characterize their vascular phenotype. Superoxide anion was significantly higher in microvessels isolated from the cortex and hippocampus as compared to the cerebellum. Importantly, protein levels of NADPH oxidase (NOX)-2 and NOX-4 were significantly higher in the cortical and hippocampal microvessels as compared to microvessels from the cerebellum. In addition, expression of manganese superoxide dismutase protein was significantly lower in microvessels from the cortex and hippocampus
A summary of the article is shown below:. Prenatal inflammation is a risk factor for necrotizing enterocolitis (NEC) and it increases intestinal injury in a rat NEC model. We previously showed that maldevelopment of the intestinal microvasculature and lack of VEGFR2 signaling play a role in experimental NEC. However, whether prenatal inflammation affects the intestinal microvasculature remains unknown. In this study, mouse dams were injected intraperitoneally with lipopolysaccharide (LPS) or saline at E17. Neonatal intestinal microvasculature density, endothelial cell proliferation and intestinal VEGF-A (VEGF) and VEGFR2 proteins were assessed in vivo. Maternal and fetal serum TNF concentrations were measured by ELISA. The impact of TNF on the neonatal intestinal microvasculature was examined in vitro and in vivo, and we determined whether prenatal LPS injection worsened experimental NEC via TNF. Here we found that prenatal LPS injection significantly decreased intestinal microvascular density, ...
Purpose : Diabetic retinopathy is a common complication of diabetes mellitus. Diabetic patients experience functional deficits in dark adaptation, contrast sensitivity and color perception before the microvascular pathologies become apparent. The mechanism that mediates these visual abnormalities remains unclear. The goal of this study was to evaluate early changes in neural retinal function and in retinoid metabolism in the eye in diabetic rats. Methods : The rats were assigned to streptozotocin-induced diabetic and non-diabetic control groups. Blood glucose levels and body weights were monitored regularly. Retinal function was examined by electroretinography (ERG). Expression levels of rod opsin and visual cycle proteins such as RPE65, LRAT, STRA6 and IRBP were analyzed by Western blotting. The enzymatic activities of RPE65 and LRAT were assayed using HPLC. The expression level and localization of STRA6 in the retinal pigment epithelium (RPE) were confirmed by immunohistochemistry. The RBP4 ...
TY - JOUR. T1 - Correlation between microvascular damage and hepatic clearance in swine liver transplantation. AU - Takasu, S.. AU - Sakagami, K.. AU - Oiwa, T.. AU - Hasuoka, H.. AU - Inagaki, M.. AU - Kurozumi, Y.. AU - Yagi, T.. AU - Miyoshi, K.. AU - Matsuoka, J.. AU - Kusaka, S.. AU - Matsuno, T.. AU - Shiozaki, S.. AU - Orita, K.. PY - 1991/1/1. Y1 - 1991/1/1. UR - http://www.scopus.com/inward/record.url?scp=0026016758&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0026016758&partnerID=8YFLogxK. M3 - Article. C2 - 1990659. AN - SCOPUS:0026016758. VL - 23. SP - 707. EP - 710. JO - Transplantation Proceedings. JF - Transplantation Proceedings. SN - 0041-1345. IS - 1 I. ER - ...
Although it has become acceptable that neuroretinal cells are also affected in diabetes, vascular lesions continue to be considered as the hallmarks of diabetic retinopathy. Animal models are essential for the understanding and treatment of human diabetic retinopathy, and the mouse is intensively used as a model because of its similarity to human and the possibility to be genetically modified. However, until today not all retinal vascular lesions developed in diabetic patients have been reproduced in diabetic mice, and the reasons for this are not completely understood. In this review, we will summarize retinal vascular lesions found in diabetic and diabetic-like mouse models and its comparison to human lesions. The goal is to provide insights to better understand human and mice differences and thus, to facilitate the development of new mouse models that mimic better human diabetic retinopathy ...
The proposed study will be performed in patients with a proven lung cancer for whom induction therapy and subsequent surgical resection of any kind is planned. Kinetic analysis of dynamic contrast-enhanced CT will performed using the CT Perfusion 3 software (General Electric Medical Systems), yielding parameters characterizing tumor microvasculature in terms of the vascularity, or the blood volume (BV), the tumor perfusion or blood flow (BF), and the immaturity of the vascular wall, in terms of the microvascular permeability (permeability surface area, PS).. To test the assumption that dynamic CT-assessed tumor microvascular characteristics represent reliable, user-independent and reproducible parameters, microvascular values and parameter maps will be derived from two independent observers. To address the interobserver variability, each study will be analyzed by the PI and by a second researcher (blinded to the results of the PI). Kappa-statistics will be used to assess inter-rater ...
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Define microvasculature. microvasculature synonyms, microvasculature pronunciation, microvasculature translation, English dictionary definition of microvasculature. n. The portion of the circulatory system composed of the smallest vessels, such as the capillaries, arterioles, and venules. mi′cro·vas′cu·lar adj
The overall goal of this research program is to elucidate the role of blood rheology as a determinant of microvascular function in health and disease. To this e...
The blood-brain barrier maintains central nervous system homeostasis and limits the entry of blood-borne substances that could alter neuronal function and survival. The barrier exists predominantly at the endothelium of cerebral vascular microvessels. The cerebral vascular endothelium becomes highly specialized during the formation of the neurovascular unit early in embryonic development. The blood-brain barrier is present and functional early in fetal life. The tightness of the barrier gradually increases throughout gestation and in the newborn period. Alterations in the basolateral environment of the cerebral microvasculature can modify the blood-brain barrier properties by modulating the expression of the endothelial tight junctions and other biochemical properties of the cerebral vascular endothelium. Maturation of the blood-brain barrier late in gestation correlates with increases in endogenous corticosteroids and with exposure to exogenous corticosteroids. Several adverse fetal and ...
Effect of local blunt muscle trauma on microvascular blood flow, relative hemoglobin amount and hemoglobin O2 saturation.Animals (n = 8) were ventilated wit
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Primary Human Glomerular Microvascular Endothelial Cells or HGMECs (ACBRI 128) were initiated by decapsulated glomeruli isolated from normal human kidney cortical tissue.
Human Rectal Microvascular Endothelial Cell MicroRNA https://www.sciencepro.com.br/produtos/sc-2977 https://www.sciencepro.com.br/@@site-logo/logo-novo.png ...
Human Ovarian Microvascular Endothelial Cell Lysate https://www.sciencepro.com.br/produtos/sc-7306 https://www.sciencepro.com.br/@@site-logo/logo-novo.png ...
TY - JOUR. T1 - Microvessels in diabetic neuropathy. T2 - Reply from the author. AU - Dyck, Peter J. PY - 1987. Y1 - 1987. UR - http://www.scopus.com/inward/record.url?scp=84935314340&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=84935314340&partnerID=8YFLogxK. M3 - Article. AN - SCOPUS:84935314340. VL - 37. SP - 1569. JO - Neurology. JF - Neurology. SN - 0028-3878. IS - 9. ER - ...
Physical activity -- or lack thereof -- may be related to subclinical signs of cardiovascular risk in 6-year-olds, as indicated by changes in retinal microvasculature, researchers found.
PELOBIOTECH GmbH offers a broad range of primary cells, stem cells, genetically modified cell systems and 3D cell models. To be able to offer you these premium products we work together with companies worldwide. This will guarantee best quality and a huge number of highly innovative products and services ...
We consider a nonlocal convection-diffusion equation $u_t=J*u-u-uu_x,$ where $J$ is a probability density. We supplement this equation with step-like initial conditions and prove the convergence of the corresponding solutions towards a rarefaction wave, i
TY - JOUR. T1 - Retinal microvascular abnormalities and blood pressure in older people. T2 - The Cardiovascular Health Study. AU - Wong, Tien Yin. AU - Hubbard, L. D.. AU - Klein, R.. AU - Marino, E. K.. AU - Kronmal, R.. AU - Sharrett, A. R.. AU - Siscovick, D. S.. AU - Burke, G.. AU - Tielsch, J. M.. PY - 2002/9. Y1 - 2002/9. N2 - Aim: To examine the relation between blood pressure and retinal microvascular abnormalities in older people. Methods: The Cardiovascular Health Study is a prospective cohort study conducted in four US communities initiated in 1989 to 1990. Blood pressure was measured according to standardised protocols at each examination. During the 1997-8 examination, retinal photographs were taken of 2405 people aged 69-97 years (2056 without diabetes and 349 with diabetes). Signs of focal microvascular abnormalities (focal arteriolar narrowing, arteriovenous nicking, and retinopathy) were evaluated from photographs according to standardised methods. To quantify generalised ...
Cardiac microvascular function abnormalities in Fabry patients have been demonstrated by measurements of myocardial blood flow and coronary flow reserve, an index of microvascular function.14 In addition, a survey of female Fabry patients revealed that cardiac ischemia could be confirmed by ECG and serological markers in the absence of coronary artery stenosis, suggesting that the ischemia in these patients was of microvascular origin.11 However, widespread coronary artery disease also has been documented in Fabry patients15 and noted at autopsy in a male patient who died of a massive myocardial infarction.16 In the present study, we characterized the baseline pathology of GL-3 accumulation in cardiac biopsies and demonstrated its successful clearance from the microvasculature after enzyme replacement therapy.. It has long been established that GL-3 is transported in low- and high-density lipoprotein particles17-19 and that vascular cells accumulate GL-3 from the circulation through the ...
Offspring of hypertensive pregnancies are more likely to have microvascular rarefaction and increased blood pressure in later life. We tested the hypothesis that maternal angiogenic profile during a hypertensive pregnancy is associated with fetal vasculogenic capacity and abnormal postnatal microvascular remodeling. Infants (n=255) born after either hypertensive or normotensive pregnancies were recruited for quantification of postnatal dermal microvascular structure at birth and 3 months of age. Vasculogenic cell potential was assessed in umbilical vein endothelial cells from 55 offspring based on in vitro microvessel tube formation and proliferation assays. Maternal angiogenic profile (soluble fms-like tyrosine kinase-1, soluble endoglin, vascular endothelial growth factor, and placental growth factor) was measured from postpartum plasma samples to characterize severity of pregnancy disorder. At birth, offspring born after hypertensive pregnancy had similar microvessel density to those born after a
Dopexamine may be a therapeutic option to improve hepatosplanchnic perfusion in sepsis. To investigate this possibility, we administered dopexamine in an experimental sepsis model in rats. This prospective, randomized, controlled laboratory study was conducted in 42 Wistar rats. The animals were divided into three groups. Group 1 served as the control group (CON group). The animals in both groups 2 (LPS group) and 3 (DPX group) received an endotoxin (lipopolysaccharide from Escherichia coli - LPS) infusion (20 mg/kg for 15 minutes). DPX group additionally received dopexamine (0.5 μg/kg per minute over four hours). One half of the animals in each group underwent studies of intestinal microvascular blood flow (IMBF) using laser Doppler fluxmetry. In the other half an intravital microscopic evaluation of leucocyte-endothelial cell interaction in intestinal microcirculation was conducted. Functional capillary density (FCD) in the intestinal mucosa and in the circular as well as longitudinal muscle layer
TY - JOUR. T1 - Injury of the renal microvascular endothelium alters barrier function after ischemia. AU - Sutton, Timothy A.. AU - Mang, Henry E.. AU - Campos, Silvia B.. AU - Sandoval, Ruben M.. AU - Yoder, Mervin C.. AU - Molitoris, Bruce A.. PY - 2003/8/1. Y1 - 2003/8/1. N2 - The role of renal microvascular endothelial cell injury in the pathophysiology of ischemic acute renal failure (ARF) remains largely unknown. No consistent morphological alterations have been ascribed to the endothelium of the renal microvasculature as a result of ischemia-reperfusion injury. Therefore, the purpose of this study was to examine biochemical markers of endothelial injury and morphological changes in the renal microvascular endothelium in a rodent model of ischemic ARF. Circulating von Willebrand factor (vWF) was measured as a marker of endothelial injury. Twenty-four hours after ischemia, circulating vWF peaked at 124% over baseline values (P = 0.001). The FVB-TIE2/GFP mouse was utilized to localize ...
We investigated whether acute FFA elevation in lean women impairs, and whether overnight FFA lowering in obese women improves, microvascular function in the basal state and during physiological hyperinsulinemia. We report four novel findings: 1) in lean women, acute elevation of FFA levels impairs microvascular function (capillary recruitment and acetylcholine-induced vasodilation) both in the basal state and during hyperinsulinemia, 2) in obese women, overnight lowering of FFA levels improves capillary recruitment both in the basal state and during hyperinsulinemia, 3) in lean and obese women, changes in FFA levels are inversely associated with changes in capillary recruitment and insulin-mediated glucose uptake, and 4) in lean and obese women, changes in microvascular function can statistically explain part of the association between changes in FFA levels and changes in insulin-mediated glucose uptake. These findings are consistent with a role for FFA-induced microvascular dysfunction in the ...
NADPH oxidase 2 (Nox2)-derived oxidative stress and redox-signalling have been found to play an important role in hyperglycaemia-induced endothelial dysfunction in diabetes. Acetate (NaA) is a member of the short chain fatty acids (SCFA) family which acts through G-protein coupled receptor 43 (GPCR43) to exert anti-inflammatory effects and to increase insulin sensitivity. However, its action in endothelial cells remains unknown. In this study we investigated the effects of NaA and GPCR43 on high glucose (30 mM, 24 h)-induced Nox2 activation and endothelial cell cycle progression using human pulmonary microvascular endothelial cells (HPMECs). Compared to control cells, high glucose increased significantly i) Nox2-derived superoxide production (48.5±12.6%) as detected by both lucigenin (5 µM)-chemiluminescence and DHE fluorescence; ii) expression of cyclin D, A and E and cell cycle progression from G0/G1 to S and G2/M phases and iii) cell apoptosis (30.66±8.3%) (all p,0.05). These high-glucose ...
Cardiovascular disease (CVD), particularly coronary vascular disease, is a primary contributor to diabetes-related morbidity and mortality worldwide. Specifically, dysfunction of coronary microcirculation is common in this patient population, often occurring in the absence of or preceding epicardial coronary atherosclerosis, thereby leading to impaired coronary blood flow (CBF) regulation and increased risk of myocardial ischemia/infarct. Clinically, coronary microvascular function can be assessed by determination of coronary flow reserve (CFR) (ratio of maximal hyperemic to basal CBF), and impaired CFR is a powerful independent correlate of cardiac mortality in diabetic patients (1). Importantly, recent evidence revealed that diabetic patients with preserved CFR (above the median) have cardiac event rates similar to nondiabetic patients (1). Thus, treatment strategies designed to restore CFR (i.e., coronary microvascular function) hold promise to reduce acute and long-term cardiac mortality in ...
ATCC hTERT immortalized dermal microvascular endothelial cells have an extended lifespan, express a panel of endothelial cell surface proteins, undergo tubule formation in culture and are karyotypically, morphologically, and phenotypically similar to the primary parent cells.
TY - JOUR. T1 - Aligned human microvessels formed in 3D fibrin gel by constraint of gel contraction. AU - Morin, Kristen T.. AU - Smith, Annie O.. AU - Davis, George E.. AU - Tranquillo, Robert T.. N1 - Funding Information: This work was supported by the National Institutes of Health [ R01 HL108670 to RTT]; and the American Heart Association [ 11PRE7610056 to KTM]. PY - 2013/11. Y1 - 2013/11. N2 - This study aimed to form microvessels in fibrin gels, which is of interest both for studying the fundamental cell-matrix interactions as well as for tissue engineering purposes, and to align the microvessels, which would provide natural inlet and outlet sides for perfusion. The data reported here demonstrate the formation of highly interconnected microvessels in fibrin gel under defined medium conditions and the ability to align them using two methods, both of which involved anchoring the gel at both ends to constrain the cell-induced compaction. The first method used only defined medium and resulted ...
Keske, Michelle, Barrett, EJ, Lindner, JR, Clark, MG and Rattigan, S 2003, Inhibiting NOS blocks microvascular recruitment and blunts muscle glucose uptake in response to insulin., American journal of physiology. Endocrinology and metabolism, vol. 285, no. 1, pp. E123-E129, doi: 10.1152/ajpendo.00021.2003. ...
Coronary microvascular disease is a condition that affects the walls and lining of the tiny blood vessels that branch off of the larger coronary arteries. The condition primarily affects women. If you suspect a problem, call 714-456-6699.
The findings from these studies expand our understanding of the role lung MPCs, and the pericytes derived from them, play during pulmonary microvascular homeostasis and adaptive angiogenesis following injury. We demonstrate that ABCG2+ MPCs directly influence lung microvascular function. Specifically, this work delineates that the coordinated regulation of Wnt/β-catenin signaling in ABCG2+ mesenchymal pericyte progenitors, autonomously or downstream of BMPR/TGF-β signaling, is a key determinant of lung microvascular integrity and is intimately linked with the alveolar epithelium. Indeed, increased canonical Wnt signaling enhanced ABCG2+ MPC proliferation and promoted the specification of these cells to functionally deficient, proangiogenic pericytes, in lieu of their active contribution to myofibroblast accumulation with fibrosis (Figure 6). Importantly, these findings affirm that maintaining a proper balance of Wnt signaling in lung ABCG2+ MPCs promotes proper pericyte development for the ...
A complete solution to propagate dermal microvascular cells in low serum (5% FBS) conditions with or without the addition of human recombinant VEGF.
The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36 ...
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Sigma-Aldrich offers abstracts and full-text articles by [Nisarga Naik, Donny Hanjaya-Putra, Carolyn A Haller, Mark G Allen, Elliot L Chaikof].
Microvascular obstruction remains a portent of adverse remodeling in optimally treated patients with left ventricular systolic dysfunction after acute myocardial infarction.
Tumor angiogenesis is the process through which certain tumors stimulate the growth of the microvascular network in the surrounding tissue. This capillary network is remarkable in that the growth is...
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Resistance to blood flow in microvessels in vivo. Pries AR, Secomb TW, Gessner T, Sperandio MB, Gross JF, Gaehtgens P. Circ Res ...
Baez S, Feldman SM, Gootman PM (July 1977). "Central neural influence on precapillary microvessels and sphincter". The American ...
The microvessels include terminal arterioles, metarterioles, capillaries, and venules. Arterioles carry oxygenated blood to the ... The microcirculation is the circulation of the blood in the smallest blood vessels, the microvessels of the microvasculature ...
Brighton, Carl T.; Hunt, Robert M. (1997). "Early histologic and ultrastructural changes in microvessels of periosteal callus ...
FLS Grau, Georges E. (December 1999). "Platelet Accumulation in Brain Microvessels in Fatal Pediatric Cerebral Malaria". The ...
October 2001). "Occludin expression in microvessels of neoplastic and non-neoplastic human brain". Neuropathology and Applied ...
"Microvessels-on-a-Chip to Assess Targeted Ultrasound-Assisted Drug Delivery". ACS Applied Materials & Interfaces. 8 (46): 31541 ...
The Enterprise is ambushed and destroyed by countless alien micro-vessels; the crew abandon ship. Stranded on an unknown planet ...
Complications include the severing of nerves and microvessels, pain, and bruising. In some cases, hyaluronic acid fillers can ...
"Molecular anatomy of the cerebral microvessels in the isolated guinea-pig brain". Brain Res. 999 (1): 81-90. doi:10.1016/j. ...
"Systematic evolution of a DNA aptamer binding to rat brain tumor microvessels. selective targeting of endothelial regulatory ...
With time, these clusters eventually migrate towards microvessels in the subgranular zone. As the clusters get closer to the ...
The conjunctiva is highly vascularised, with many microvessels easily accessible for imaging studies. The conjunctiva is ...
"Sickle Erythrocytes Target Cytotoxics to Hypoxic Tumor Microvessels and Potentiate a Tumoricidal Response". PLOS ONE. 8 (1): ...
Under stressful conditions, MLCK's in the human body promotes increased permeability of microvessels. It is thought that MLCK ...
2007). "Relationship between vascular invasion and microvessel density and micrometastasis". World J. Gastroenterol. 13 (46): ...
The mineralized matrix is penetrated by microvessel and numerous osteoblasts. The osteoblasts form new lamellar bone upon the ... "Early histologic and ultrastructural changes in microvessels of periosteal callus", Journal of Orthopaedic Trauma, 11 (4): 244- ...
"Modeling hyperosmotic blood-brain barrier opening within human tissue-engineered in vitro brain microvessels". Journal of ...
"Loss of vascular adhesion protein-1 expression in intratumoral microvessels of human skin melanoma". Melanoma Research. 14 (2 ...
Star Trek Beyond (2016) - the Enterprise is ambushed and destroyed by countless alien microvessels; the crew abandon ship and ...
Zhang W, Yang H, Han S (September 1998). "[The effect of ectopic HCG on microvessel density in gastric carcinoma]". Zhonghua ...
"Desmin expression in colorectal cancer stroma correlates with advanced stage disease and marks angiogenic microvessels". Clin ...
It is widely accepted EDHF plays an important role in vasotone, especially in micro vessels. Its effect varies, depending on ...
"Effects of crocin on reperfusion-induced oxidative/nitrative injury to cerebral microvessels after global cerebral ischemia". ...
These microvessels are the site of exchange of many substances with the interstitial fluid surrounding them. Carcinogen - is ...
These microvessels are the site of exchange of many substances with the interstitial fluid surrounding them. Substances which ...
There is a correlation between expression of egfl7, cell proliferation and micro-vessel density. Silencing (knockdown) of the ... In mice, inhibition of egfl7 in hepatocellular carcinoma cells decrease tumour growth and micro-vessel density. Over-expression ... Within the tumours, Egfl7 increases micro-vessel density, hypoxia, necrosis and vascular permeability. Egfl7 is a natural ...
"Expression of non-mast cell histidine decarboxylase in tumor-associated microvessels in human esophageal squamous cell ...
Tsuji, T., Mimori, Y., Nakamura, S., and Kameyama, M., 1987, A micromethod for the isolation of large and small microvessels ... Tsuji T., Mimori Y., Nakamura S. (1990) Acetylcholinesterase of Cerebral Microvessels Changes in Alzheimers Disease. In: ... Vascular cholinesterases and choline uptake in isolated rat forebrain microvessels: A possible link, J. Neurochem., 53: 561. ... AChE might possibly be coupled with choline transport in microvessels isolated from the rat forebrain (Shimon et al., 1989). ...
Two forms of gamma-glutamyltransferase from human brain cortex microvessels were partially purified by gel permeation and ion- ... Solubilization and some properties of γ-glutamyltransferase from human brain microvessels. Neurochem. Res. 9:927-934.PubMed ... Two forms of gamma-glutamyltransferase from human brain cortex microvessels were partially purified by gel permeation and ion- ...
Average blood perfusion rate in the target microvessels before and after PUT treatment in 5 rabbits (**p , 0.001, ***p , 0.0001 ... As a result, PUT can offer deep penetration and unprecedented high precision for treating microvessels beyond the skin surface ... The findings demonstrate that PUT can selectively treat veins and arteries, and microvessels beyond the surface of optically ... The shear stresses and microjets produced by oscillating cavitation bubbles in a microvessel can directly impact the ...
Properties of angiotensin-converting enzyme in intact cerebral microvessels.. P Brecher, A Tercyak, A V Chobanian ... Angiotensin I was rapidly degraded by the intact microvessels, even in the presence of 10(-6)M captopril. An advantage of the ... The ability to measure ACE activity of the microvessels in vitro provides a unique opportunity to study the properties of the ... Activity was determined by measuring the degradation of hippuryl-histidyl-leucine (Hip-His-Leu) by the intact microvessels in a ...
Biosynthesis of prostacyclin in rat cerebral microvessels and the choroid plexus.. Goehlert UG, Ng Ying Kin NM, Wolfe LS. ... Microvessels, predominantly capillaries, were isolated from rat cerebrum by a modification of published procedures. The ... and F2 alpha showed that 6-keto-prostaglandin F1 alpha was the major prostaglandin formed in the microvessels, in the larger ...
The traffic of polymorphonuclear leukocytes through pulmonary microvessels in health and disease.. Hogg JC1. ... traffic[Title] AND polymorphonuclear[Title] AND leukocytes[Title] AND pulmonary[Title] AND microvessels[Title] AND health[Title ... Search: traffic[Title] AND polymorphonuclear[Title] AND leukocytes[Title] AND pulmonary[Title] AND microvessels[Title] AND ...
Patrolling Monocytes Are Recruited and Activated By Diabetes To Protect Retinal Microvessels ... Patrolling Monocytes Are Recruited and Activated By Diabetes To Protect Retinal Microvessels ... Patrolling Monocytes Are Recruited and Activated By Diabetes To Protect Retinal Microvessels ... Patrolling Monocytes Are Recruited and Activated By Diabetes To Protect Retinal Microvessels ...
... Int J Oral Maxillofac Surg. 2015 Mar;44(3 ... The samples were subjected to immunohistochemistry staining: double staining with CD31 and D2-40 for microvessels, or Ki-67 for ...
Effect of Ethanol on Micro-Vessels Diameter and Prevention of Thrombosis. Author(s): ...
Dette manuskriptet presenterer en injeksjon metode for å konstruere microvessels at rekapitulere fysiologiske egenskapene til ... Zheng, Y., Chen, J., et al. In vitro microvessels for the study of angiogenesis and thrombosis. Proc. Natl. Acad. Sci. U. S. A ... Den beskrevne metoden genererer konstruerte microvessels med diameter i størrelsesorden 100 mikrometer. Konstruert microvessels ... Zheng, Y., Chen, J., Lòpez, J. A. Flow-driven assembly of VWF fibres and webs in in vitro microvessels. Nat. Commun. 6, 7858 ( ...
Zheng, Y., Chen, J., et al. In vitro microvessels for the study of angiogenesis and thrombosis. Proc. Natl. Acad. Sci. U. S. A ... Zheng, Y., Chen, J., Lòpez, J. A. Flow-driven assembly of VWF fibres and webs in in vitro microvessels. Nat. Commun. 6, 7858 ( ... Roberts, M. A., Kotha, S. S., Phong, K. T., Zheng, Y. Micropatterning and Assembly of 3D Microvessels . J. Vis. Exp. (115), ... Roberts, M. A., Kotha, S. S., Phong, K. T., Zheng, Y. Micropatterning and Assembly of 3D Microvessels . J. Vis. Exp. (115), ...
The endothelium partially obscures enhanced microvessel reactivity in DOCA hypertensive rats.. C M King, R C Webb ...
Engineered Microvessel Structure Provides More Accurate Testbed for Studying Human Diseases. May 30th, 2012 Scott Jung News ... Researchers made a functional microvessel that spells the letters UW. The white bar measures 100 micrometers, about the width ... Article from the University of Washington: Engineered microvessels provide a 3-D test bed for human diseases…. ...
Angiophagy Prevents Early Embolus Washout But Recanalizes Microvessels Through Embolus Extravasation Message Subject. (Your ... Angiophagy Prevents Early Embolus Washout But Recanalizes Microvessels Through Embolus Extravasation. By Jaime Grutzendler, ... Angiophagy Prevents Early Embolus Washout But Recanalizes Microvessels Through Embolus Extravasation. By Jaime Grutzendler, ...
... J Pathol. 1992 Mar;166(3):265-70. ... as well as from those of patients with infarcts and no diabetes were used for morphometric studies of intramural microvessels ...
Our previous study demonstrated that ROS release from fMLP-stimulated neutrophils increases microvessel permeability ... These results indicate that ROS-induced increases in microvessel permeability involve the activation of a NO-cGMP-dependent ... Changes in permeability after microvessels were perfused with fMLP-stimulated neutrophil suspensions with and without the ... Reactive oxidant species (ROS) release from fMLP-stimulated neutrophils increases microvessel permeability via nitric oxide (NO ...
Mitosis-mediated intravasation in a tissue-engineered tumor-microvessel platform. Andrew D. Wong and Peter C. Searson ... Mitosis-mediated intravasation in a tissue-engineered tumor-microvessel platform Message Subject (Your Name) has forwarded a ... Here we analyzed the intravasation of invasive human breast cancer cells within a tissue-engineered microvessel model of the ... Using live-cell fluorescence microscopy, we captured 2,330 hours of tumor cell interactions with functional microvessels and ...
Resistance to blood flow in microvessels in vivo.. A R Pries, T W Secomb, T Gessner, M B Sperandio, J F Gross, P Gaehtgens ... For microvessels with diameters below approximately 40 microns, the resulting flow resistances are markedly higher and show a ... For example, flow resistance in 10-microns microvessels at normal hematocrit is found to exceed that of a corresponding glass ... The unexpectedly high flow resistance in small microvessels may be related to interactions between blood components and the ...
The microvessel technology will help to create new tissues and provide an important tool for evaluating new therapies. ... The microvessel technology will help to create new tissues and provide an important tool for evaluating new therapies. ... The multi-layered microvessels combine polymers that are compatible with human cells, but also encourage cell growth and ... Human cells of a specific type can be mixed with appropriately customized materials destined for each layer of the microvessel ...
Microvessel Analysis. Reconstruct and perform complex analyses of microvessels. Researchers and clinicians are increasingly ... Microvessel reconstruction and 3D rendering however is computationally intensive due to the density of microvessels, ... Microvessel quantitative analysis is performed to study. *Cerebral angiogenesis following ischemic stroke or trauma ... As the body of knowledge in microvessel anatomy and physiology continues to expand, the scientific community has sought to ...
β1-integrin-matrix interactions modulate cerebral microvessel endothelial cell tight junction expression and permeability. ... β1-integrin-matrix interactions modulate cerebral microvessel endothelial cell tight junction expression and permeability. ...
Microvessel permeability was determined by measuring hydraulic conductivity (Lp). Endothelial [Ca(2+)](i), a necessary signal ... The effects of cell-free, spent culture medium from virulent (B31-A3) and avirulent (B31-A) B. burgdorferi on microvessel ... Spent culture medium from virulent Borrelia burgdorferi increases permeability of individually perfused microvessels of rat ... that can directly activate endothelial cells resulting in increases in permeability in intact microvessels in the absence of ...
2 and NOX-4 were significantly higher in the cortical and hippocampal microvessels as compared to microvessels from the ... We isolated microvessels from wild type mice from the cerebellum, cortex, and hippocampus to characterize their vascular ... In this study, we sought to determine if regional heterogeneity of cerebral microvessels might help explain the susceptibility ... Superoxide anion was significantly higher in microvessels isolated from the cortex and hippocampus as compared to the ...
Immunohistochemical analysis of mast cell infiltrates and microvessel density in oral squamous cell carcinoma. Download Prime ... Carcinoma, Squamous CellFemaleHumansImmunohistochemistryMaleMast CellsMicrovesselsMiddle AgedMouthMouth Neoplasms ... Immunohistochemical Analysis of Mast Cell Infiltrates and Microvessel Density in Oral Squamous Cell Carcinoma. Pol J Pathol. ... Immunohistochemical analysis of mast cell infiltrates and microvessel density in oral squamous cell carcinoma. Pol J Pathol. ...
Enhancement of platelet aggregation by tranylcypromine in mouse cerebral microvessels.. W I Rosenblum, F El-Sabban ...
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Abstract 8993: Protective Effects of Glucagon-like Peptide-1 on Cardiac Microvessels Injury via cAMP/PKA/Rho Dependent Pathway ... Hypothesis: To investigate the protective effects of GLP-1 on cardiac microvessels injury and the underlying mechanism in ... Abstract 8993: Protective Effects of Glucagon-like Peptide-1 on Cardiac Microvessels Injury via cAMP/PKA/Rho Dependent Pathway ... Abstract 8993: Protective Effects of Glucagon-like Peptide-1 on Cardiac Microvessels Injury via cAMP/PKA/Rho Dependent Pathway ...
SPACEFLIGHT AND RADIATION INDUCES MICROVESSEL AND STRUCTURAL DAMAGE IN OCULAR TISSUE. 9th Global Ophthalmology Summit. March 15 ... The role of antioxidant MnTE-2-PyP in protecting the ocular tissue and retinal microvessels from radiation damage was also ...
CONCLUSIONBoth iron deposits and microvessels contribute to the hyposignal surrounding nigrosome 1 in susceptibility weighted ... Microvessels may Confound the "Swallow Tail Sign" in Normal Aged Midbrains: A Postmortem 7 T SW‐MRI Study. *. ... CONCLUSIONBoth iron deposits and microvessels contribute to the hyposignal surrounding nigrosome 1 in susceptibility weighted ...
However, the study of microvessels using CT has been exceptionally difficult due to their small (, 10 µm) size, lack of ... Currently, the effects of changes in the subchondral bone microvessel density on cartilage health remain unknown due to the ... Results gained from this study will further provide information into the role that microvessels may play in OA. ... difficulties in simultaneously studying dense bone and the associated small microvessels. Computed tomography (CT) is widely ...
  • Microvessel reconstruction and 3D rendering however is computationally intensive due to the density of microvessels, necessitating powerful computer graphics systems and complicated algorithms beyond the expertise of most life science research laboratories. (mbfbioscience.com)
  • The aim of the study was to evaluate mast cell concentration and microvessel density in perilesional and intralesional regions of oral squamous cell carcinoma (OSCC) and furthermore to assess the possible relationship between the above-mentioned parameters. (unboundmedicine.com)
  • The study revealed considerably increased microvessel density and mast cell abundance in intralesional and perilesional regions of OSCCs in comparison to normal mucosa. (unboundmedicine.com)
  • Currently, the effects of changes in the subchondral bone microvessel density on cartilage health remain unknown due to the difficulties in simultaneously studying dense bone and the associated small microvessels. (uwo.ca)
  • We also focus on few studies that have evaluated the correlation between RCC proliferation, MC count and microvessel density (MVD), as hallmarks of tumor angiogenesis. (mdpi.com)
  • Results from traditional manual microvessel density (MVD) hotspot count and computerized hotspot count were compared and the relation between hotspot MVD count and survival rate was evaluated. (aacrjournals.org)
  • In our studies, we reported that hotspot microvessel density (MVD) in biopsy specimen is of strong prognostic significance for patients with laryngeal squamous cell cancers and with hypopharyngeal cancers treated with radiation ( 7 , 8 ). (aacrjournals.org)
  • Microvessel density values in pre-Tam biopsies were significantly higher than corresponding values in posttreatment tissues ( z = −3.72, P = 0.0002). (aacrjournals.org)
  • The reduction in the percentage of Ki67-positive tumors was significantly ( z = 3.58, P = 0.0003) higher in ER-positive than in ER-negative tumors, whereas no difference in Tam-induced reduction of microvessel density values according to ER status ( z = −0.18, P = 0.85) was found. (aacrjournals.org)
  • To investigate whether lymphatic microvessel density (LMVD) could be used as a predictive marker for the recurrence time of pterygia. (molvis.org)
  • In our previous study, we demonstrated with enzyme histochemistry [ 16 ] and immunohistochemistry [ 17 ] that lymphatic microvessel density (LMVD) increased dramatically with the severity of pterygia. (molvis.org)
  • Aims We aimed to determine whether cancer-associated fibroblasts (CAFs) are associated with microvessel density (MVD) and lymphatic vessel density (LVD) in lung squamous cell carcinoma, as well as their clinical significance in predicting survival. (bmj.com)
  • In the present study, we investigated the relationship between CHI3L1 expression and microvessel density (MVD), a reflection of angiogenesis, with metastasis and prognosis in patients with clear cell renal cell carcinoma (RCC). (springermedizin.de)
  • The aim of this study was to examine the relationship between tumour necrosis, tumour proliferation, local and systemic inflammation and microvessel density and survival in patients undergoing potentially curative resection of oesophageal adenocarcinoma. (gla.ac.uk)
  • Expression of vascular endothelial growth factor (VEGF) and association with microvessel density in benign prostatic hyperplasia and prostate cancer. (semanticscholar.org)
  • Growth index is independent of microvessel density in non-small cell lung carcinomas. (semanticscholar.org)
  • 2015). 'Microvessel Density as a Prognostic Factor in Ovarian Cancer: a Systematic Review and Meta-analysis', Asian Pacific Journal of Cancer Prevention , 16(3), pp. 869-874. (waocp.org)
  • The prognostic value of microvessel density (MVD), reflecting angiogenesis, detected in ovariancancer is currently controversial. (waocp.org)
  • The studies were categorized by author/year, number of patients,FIGO stage, histology, cutoff value for microvessel density, types of survival analysis, methods of hazard rations(HR) estimation, HR and its 95% confidence interval (CI). (waocp.org)
  • We investigated Activin A and Follistatin in serum and tumor tissue of patients with TETs in relation to microvessel density (MVD), WHO histology classification, tumor stage and outcome. (nature.com)
  • A significant correlation between microvessel density (MVD) according to RECK and CD34 was revealed. (imedpub.com)
  • Our study reported that RECK is expressed in endothelial cells in gliomas and that microvessels density (MVD) expressed by RECK is positively correlated with MVD expressed by CD34 and the glioma grade [ 13 ]. (imedpub.com)
  • The immortalized human cerebral microvessel endothelial cell line hCMEC/D3 has been repeatedly used as a model of human blood-brain barrier (BBB). (open.ac.uk)
  • A quantitative description of motility of individual cells would be useful, for example, in the study of the formation of new blood vessel networks in angiogenesis by microvessel endothelial cell (MEC) migration. (biologists.org)
  • To address this issue, the expression level of two proteins, P-gp and heat shock protein 70 (Hsp70), and their effects on uptake of R123 and permeability of R123 and [14C]-sucrose in combination with mild heat and P-gp modulator PSC833 during and after mild heat treatment in heat-preconditioned and heat-unconditioned bovine brain microvessel endothelial cell (BBMEC) monolayers were studied. (oregonstate.edu)
  • Single perfused rat mesenteric microvessels were perfused with fluorescent endothelial cell membrane and glycocalyx labels, and imaged with confocal microscopy. (nottingham.ac.uk)
  • To evaluate the hypothesis that sphingosine-1-phosphate (S1P) and cAMP attenuate increased permeability of individually perfused mesenteric microvessels through a common Rac1-dependent pathway, we measured the attenuation of the peak hydraulic conductivity (L p ) in response to the inflammatory agent bradykinin (BK) by either S1P or cAMP. (elsevier.com)
  • The first studies demonstrated microcirculatory effect of glycine solution on mesenteric microvessels and on pial arterioles in laboratory rats [4-6]. (peertechz.com)
  • Images of leukocyte rolling in mesenteric microvessels were investigated in a fluorescence bio-imaging model. (scirp.org)
  • Results: 1) Leukocyte rolling in mesenteric microvessels was significantly increased in colitis. (scirp.org)
  • Activity was determined by measuring the degradation of hippuryl-histidyl-leucine (Hip-His-Leu) by the intact microvessels in a physiological salt solution at pH 7.4. (ahajournals.org)
  • Angiotensin I was rapidly degraded by the intact microvessels, even in the presence of 10(-6)M captopril. (ahajournals.org)
  • The objective of the present study was to investigate if Bb produces a mediator(s) that can directly activate endothelial cells resulting in increases in permeability in intact microvessels in the absence of blood cells. (prohealth.com)
  • The overall aim of this project is to investigate the cellular mechanisms involved in permeability modulation in intact microvessels. (elsevier.com)
  • The unique advantages of the approach are that all of the experiments will be conducted in intact microvessels, which have normal permeability properties, and that the single microvessel perfusion technique will enable the direct effect of NO on changes in permeability to be separated from its hemodynamic effect as a vasodilator. (elsevier.com)
  • The investigator has developed a novel method to delineate endothelial boundaries with silver precipitation in vivo, which provides a mapping tool for the study of calcium signaling in individual endothelial cells in intact microvessels. (elsevier.com)
  • This degree of cellular localization in intact microvessels could not be achieved by previous methods. (elsevier.com)
  • Changes in permeability after microvessels were perfused with fMLP-stimulated neutrophil suspensions with and without the inhibition of endothelial eNOS or guanylate cyclase were measured as hydraulic conductivity in individually perfused rat mesenteric venular microvessels. (cdc.gov)
  • The effects of cell-free, spent culture medium from virulent (B31-A3) and avirulent (B31-A) B. burgdorferi on microvessel permeability and endothelial calcium concentration, [Ca(2+)](i), were examined in individually perfused rat mesenteric venules. (prohealth.com)
  • Human coronary microvessels in diabetes and ischaemia. (nih.gov)
  • In order to address this problem, a protein transfection technique we developed for cultured endothelial cells has been modified and applied to isolated, intact coronary microvessels. (ovid.com)
  • Vasomotor tone was studied in coronary microvessels from healthy control swine and from swine 3 to 4 months after implantation of an occluder that causes a progressive coronary narrowing, resulting in regional left ventricular dysfunction and blunted myocardial vasodilator reserve. (eur.nl)
  • In conclusion, inward remodeling of coronary microvessels distal to a stenosis is accompanied by exaggerated vasoconstrictor responses to endothelin-1. (eur.nl)
  • Pathophysiology and therapeutic strategy of coronary vasospasm of microvessels using a novel porcine model. (nii.ac.jp)
  • The unexpectedly high flow resistance in small microvessels may be related to interactions between blood components and the inner vessel surface that do not occur in smooth-walled tubes. (ahajournals.org)
  • A critical role in this complex pathology was assigned to tubulointerstitial blood microvessels that regulate the supply of oxygen and nutrients of tubuli. (asnjournals.org)
  • Pericytes are vascular mural cells embedded in the basement membrane of blood microvessels. (freepatentsonline.com)
  • The aim of present work was to study if there is any dependence of Glycine effect on the pH values and the arteriole (blood microvessels) diameter size. (peertechz.com)
  • Thus, RECK may act during angiogenesis in glioblastomas, especially in forming simple vessels as microvessels rather than glomeruloid vessels. (imedpub.com)
  • The use of confocal microscopy will enable local changes in microvessel permeability and individual endothelial [Ca2=]I to be measured and the vascular structure to be identified in vivo simultaneously. (elsevier.com)
  • To investigate the mechanisms of how surface properties of the endothelial cells control the changes in microvessel permeability, we extended the charge-diffusion model developed by Fu et al. (unlv.edu)
  • These results indicate that ROS-induced increases in microvessel permeability involve the activation of a NO-cGMP-dependent signaling pathway, which can be counteracted by enhancing endothelial cAMP levels. (cdc.gov)
  • A mediator(s) produced by virulent Bb under culture conditions directly activates endothelial cells, resulting in increases in microvessel permeability. (prohealth.com)
  • Resistance to blood flow in microvessels in vivo. (ahajournals.org)
  • In vivo, CD44 v3+ microvessels have been observed in normal skin, glottic tissue, in head and neck cancers as well as in melanoma tissue samples. (aacrjournals.org)
  • We interpret these observations to show (1) these human PBL lines maintained their phenotype following in vivo restimulation, and (2) noncytolytic graft-infiltrating lymphocytes specifically promote injury of allogeneic human microvessels. (mysciencework.com)
  • We developed novel, real time, paired methodologies to study the contribution of sialic acids within the endothelial glycocalyx to the structural and functional permeability properties of the same microvessel in vivo. (nottingham.ac.uk)
  • In the brain, various caveolin subtypes have been detected in vivo by immunocytochemistry: caveolin-1 and -2 were found in rat brain microvessels, caveolin-3 was revealed in astrocytes. (icr.ac.uk)
  • Similar results were obtained with inhibition of endothelial eNOS activity by internalization of caveolin-1 scaffolding domain in individually perfused microvessels. (cdc.gov)
  • Spent culture medium from virulent Borrelia burgdorferi increases permeability of individually perfused microvessels of rat mesentery. (prohealth.com)
  • The aim of this study was to identify the site(s) of cellular expression of caveolin-1 in the microvessels of the human cerebral cortex by immunofluorescence confocal microscopy and immunogold electron microscopy. (icr.ac.uk)
  • Soon after the onset of diabetes, insulin failed to significantly diminish cell death in ischemic retinal microvessels, in contrast to the prosurvival effect of this hormone on nondiabetic microvessels. (arvojournals.org)
  • Results of this study support the hypothesis that a physiological concentration of insulin exerts a prosurvival effect on ischemic retinal microvessels and that diabetes impairs the ability of insulin to protect against ischemia-induced microvascular cell death. (arvojournals.org)
  • We report that physiological concentrations of insulin markedly decreased ischemic cell death in pericyte-containing microvessels of the normal rat retina. (arvojournals.org)
  • Introduction: In mouse models of Duchenne or Becker muscular dystrophy (BMD) lacking sarcolemmal neuronal nitric oxide synthase (nNOS), exercise paradoxically induces spasm of skeletal muscle microvessels, which can cause ischemic injury and fatigue. (ovid.com)
  • The role of antioxidant MnTE-2-PyP in protecting the ocular tissue and retinal microvessels from radiation damage was also evaluated. (omicsonline.org)
  • Cell viability in pericyte-containing retinal microvessels was assayed by trypan blue dye exclusion. (arvojournals.org)
  • Dysfunction of pericyte-containing retinal microvessels and the resultant ischemia are well-known complications of diabetes. (arvojournals.org)
  • 9 However, though pericyte-containing retinal microvessels express insulin receptors, 10 11 12 it is not well understood how deficiency in insulin or changes in insulin signaling result in damage to the microvasculature of the diabetic retina. (arvojournals.org)
  • More specifically, we hypothesized that a function of insulin is to protect pericyte-containing retinal microvessels from ischemia-induced cell death. (arvojournals.org)
  • Measurement of gene expression in human retinal microvessels by solution hybridization. (arvojournals.org)
  • To measure gene expression in human retinal microvessels, a RNA-probe excess solution hybridization assay was used. (arvojournals.org)
  • Retinal microvessels were isolated from eyes obtained within 36 hr of death, and intact RNA was extracted by the guanidine method. (arvojournals.org)
  • The prevalence of these transcripts in the retinal microvessels was 0.04 x 10(6) copies/ng RNA for PAI-1 and 0.14 x 10(6) copies/ng RNA for vWF, much less than the prevalence in human umbilical vein endothelial cells (1.93 x 10(6) and 3.90 x 10(6), respectively). (arvojournals.org)
  • The PAI-1 mRNA levels in retinal microvessels isolated from five type II diabetic patients were significantly higher than those in vessels isolated from ten age-matched controls (0.06 x 10(6) versus 0.04 x 10(6) copies/ng RNA, P less than 0.05). (arvojournals.org)
  • Computer-assisted tumor microvessel analysis is a powerful tool in predicting the outcome for patients with esophageal cancer treated with chemoradiotherapy because intraobserver and interobserver variability is minimized. (aacrjournals.org)
  • Tumor microvessel wall was thicker than that of brain. (elsevier.com)
  • Tyukina ES, Sheshegova EV, Nartsissov YR, Podoprigora GI (2017) Vasodilatory Effect of the Dissolved Glycine locally applied on Pial Microvessels. (peertechz.com)
  • One of the tests used for an assessment of Glycine direct vasodilatory effect is an intravital biomicroscopy of pial microvessels in a brain of laboratory rats. (peertechz.com)
  • The significant vasodilatory effect of glycine on microcirculation in pial microvessels in the cortex of a parietal area of the rat brain was shown. (peertechz.com)
  • Kontos, H. A. / Method for morphophysiological study of specific pial microvessels . (elsevier.com)
  • Two forms of gamma-glutamyltransferase from human brain cortex microvessels were partially purified by gel permeation and ion-exchange and group-affinity chromatography. (springer.com)
  • Study of the cortex microvessels carried out by immunoelectron microscopy confirmed that in the vascular wall caveolin-1 is expressed by endothelial cells, pericytes, and vascular astrocytes, and revealed the association of caveolin-1 with the cell caveolar compartment. (icr.ac.uk)
  • Expression of P-glycoprotein in human cerebral cortex microvessels. (icr.ac.uk)
  • Our experiments in frog and rat venular microvessels under a variety of conditions revealed the same time trend for new steady states to be established as in Michel and Phillips' experiments. (elsevier.com)
  • The traffic of polymorphonuclear leukocytes through pulmonary microvessels in health and disease. (nih.gov)
  • Inhibition of adsorptive endocytosis and transcytosis in pulmonary microvessels. (colorado.edu)
  • A phenomenon that has defied explanation for two decades is the time scale for transient reabsorption in the classic experiments of Michel and Phillips on individually perfused frog mesentery microvessels. (elsevier.com)
  • from the rate of tissue solute accumulation and the radial concentration gradient around individually perfused microvessel in frog mesentery. (asme.org)
  • Collectively, these results indicate that a large proportion of the microvessels encountered in the cortex of remnant kidneys are of lymphatic origin and cannot be discriminated by common endothelial markers, such as CD34, that are expressed by both lymphatic and blood endothelia cells. (asnjournals.org)
  • In the absence of specific markers, lymphatic microvessels were difficult to distinguish from blood capillaries. (asnjournals.org)
  • GLP-1 could protect the cardiac microvessels against oxidative stress injury and the resultant microvascular barrier dysfunction in diabetic rats, which contribute to the improvement of cardiac function. (ahajournals.org)
  • O alvo do projecto é desenvolver uma plataforma portátil, não invasora, eficaz na redução de custos e do tempo real para a monitoração da saúde microvascular (a saúde dos microvessels) dos pacientes COVID-19 em ICUs. (news-medical.net)
  • abstract = "We developed a numerical method for large-scale simulations of cellular flow in microvessels. (elsevier.com)
  • Researchers made a functional microvessel that spells the letters 'UW. (medgadget.com)
  • Using live-cell fluorescence microscopy, we captured 2,330 hours of tumor cell interactions with functional microvessels and provide evidence for a mitosis-mediated mechanism where tumor cells located along the vessel periphery are able to disrupt the vessel endothelium through cell division and detach into circulation. (aacrjournals.org)
  • hESC-ECs were used to make engineered microvessels and were found to retain structural and functional characteristics of mature endothelial cells, including patent lumen formation with intact junctions and non-thrombogenic blood interactions. (washington.edu)
  • Perfusion assays on 3D microvessels reveal previously unappreciated phenotypic heterogeneity in parasite binding to tumor necrosis factor alpha (TNF-α)-activated brain endothelial cells. (asm.org)
  • 0001) but weakly with MVD ( r = 0.32, P = .05), due to microvessel size heterogeneity. (ajnr.org)
  • For microvessels with diameters below approximately 40 microns, the resulting flow resistances are markedly higher and show a stronger dependence on hematocrit than previously estimated from measurements of blood flow in narrow glass tubes. (ahajournals.org)
  • For example, flow resistance in 10-microns microvessels at normal hematocrit is found to exceed that of a corresponding glass tube by a factor of approximately 4. (ahajournals.org)
  • Simulations were performed for tube hematocrit ranging from 20 to 45%, and microvessel diameters from 9 to 50 μm. (elsevier.com)
  • Angiotensin-converting enzyme (ACE) was studied in preparations of microvessels isolated from rabbit cerebral cortex. (ahajournals.org)
  • The binding characteristics of a monoiodinated form of vasoactive intestinal peptide (M-[125I]VIP) to the membranes of astrocytes, intraparenchymal microvessels and synaptosomes were analyzed in mouse cerebral cortex. (epfl.ch)
  • We have used intravital microscopy to study physiologically perfused microvessels in murine bone marrow (BM). (rupress.org)
  • Cortical microvessels were measured in awake mice in the vasospasm period using two photon laser scanning microscopy. (cns.org)
  • Fifteen patients with VCI due to cSVD and 15 controls underwent intravital microscopy of the sublingual microvessels. (frontiersin.org)
  • Here, we have generated an engineered three-dimensional (3D) human brain endothelial microvessel model and studied P. falciparum binding under the large range of physiological flow velocities that occur in both health and disease. (asm.org)
  • The engineered 3D human brain microvessel model provides new mechanistic insight into parasite binding and opens opportunities for further studies on malaria pathogenesis and parasite-vessel interactions. (asm.org)
  • Here, we have developed an engineered 3D human brain microvessel model that mimics the blood flow rates and architecture of small blood vessels to study how P. falciparum - infected human erythrocytes attach to brain endothelial cells. (asm.org)
  • Our model sheds new light on how P. falciparum binds within brain microvessels and provides a powerful method for future investigations of recruitment of human brain pathogens to the blood vessel lining of the brain. (asm.org)
  • Endothelin-1 is elevated in Alzheimer's disease brain microvessels and is neuroprotective. (semanticscholar.org)
  • Gene expression profiling in Alzheimer's disease brain microvessels. (semanticscholar.org)
  • Because oxidant stress-induced encephalopathies are mostly localized in the periventricular region in immature compared with older subjects, 2 we postulated that the constrictor effects of the product of peroxidation 15-F 2t -IsoP on microvessels of the periventricular brain region are more pronounced in immature than in older subjects. (ahajournals.org)
  • Effect of heat preconditioning on the uptake and permeability of R123 in brain microvessel endothelial cells during mild heat treatment. (oregonstate.edu)
  • An immediate goal was to determine whether prior mild heat treatment could render brain microvessel endothelial cells more resistant to future heat stress and affect BBB drug permeation by future ultrasound-induced mild heat (USMH) treatment. (oregonstate.edu)
  • Expression of caveolin-1 in human brain microvessels. (icr.ac.uk)
  • Since in the barrier-provided brain microvessels tight relations occur between the endothelium-pericyte layer and the surrounding vascular astrocytes, double immunostaining with caveolin-1 and the astroglia marker, glial fibrillary acidic protein, was also carried out. (icr.ac.uk)
  • The demonstration of caveolin-1 in the cells of the brain microvessels suggests that caveolin-1 may be involved in blood-brain barrier functioning, and also supports co-ordinated activities between these cells. (icr.ac.uk)
  • RECK expression was observed in endothelial cells of microvessels in normal brain and the patients with glioblastoma. (imedpub.com)
  • We investigated the activation of protein kinase C in microvessels isolated from rat brain. (elsevier.com)
  • Markovac, J & Goldstein, GW 1988, ' Transforming growth factor beta activates protein kinase C in microvessels isolated from immature rat brain ', Biochemical and Biophysical Research Communications , vol. 150, no. 2, pp. 575-582. (elsevier.com)
  • Using micro-optical sectioning tomography (MOST) with a modified Nissl staining method, we acquired five mouse brain data sets containing arteries, veins, and microvessels. (frontiersin.org)
  • ENU and normal brain microvessels were continuous and nonfenestrated. (elsevier.com)
  • Three microvessel populations may occur in brain tumors: (1) continuous nonfenestrated, (2) continuous fenestrated, and (3) discontinuous (with or without fenestrations). (elsevier.com)
  • Since structure-function properties of brain tumor microvessels will affect drug delivery, studies of microvessel function should be incorporated into clinical trials of brain tumor therapy, especially those using macromolecules. (elsevier.com)
  • caveolin-1 is also expressed by cells located in the neuropil around the microvessels and identified as astrocytes. (icr.ac.uk)
  • The ratio of total microvessel perimeter (TP) to total tumor area (TA) of biopsy specimens, the ratio of total microvessel number (TN) to TA, and the tumor hypoxic ratio calculated from microvessel distributions in biopsy specimens have been further proved to be good prognostic factors for patients with early stages of laryngeal carcinoma treated with radiation ( 9 ). (aacrjournals.org)
  • Reactive oxidant species (ROS) release from fMLP-stimulated neutrophils increases microvessel permeability via nitric oxide (NO)-cGMP-dependent pathway. (cdc.gov)
  • The α4/VCAM-1 pathway mediates selectin-independent rolling of progenitor cells in BM microvessels. (rupress.org)
  • Conclusions: These imaging data provide the first translational evidence in human patients with BMD that loss of sarcolemmal nNOS engenders exercise-induced spasm of intramuscular microvessels and further implicate the NO-cGMP pathway as a novel drug target for BMD. (ovid.com)
  • In the proposed studies, the NO-dependent signal transduction pathway in endothelial cells will be modified and, changes, in endothelial [Ca2+]I and microvessel permeability will be determined under the same experimental conditions. (elsevier.com)
  • We tested hypothesis that activation of the prostacyclin (PGI 2 ) receptor (IP receptor) signaling pathway in cerebral microvessels plays an important role in the metabolism of amyloid precursor protein (APP). (elsevier.com)
  • In the second experiment, there were no significant differences in cortical microvessel diameter between the experimental and control groups. (cns.org)
  • In a CM injection SAH model, we observed significant large vessel spasm but no cortical microvessel spasm or cerebral blood flow change. (cns.org)
  • The endothelium partially obscures enhanced microvessel reactivity in DOCA hypertensive rats. (ahajournals.org)
  • To investigate the protective effects of GLP-1 on cardiac microvessels injury and the underlying mechanism in diabetic rats. (ahajournals.org)
  • The combination of these two techniques would facilitate the simultaneous visualization and quantification of subchondral bone and microvessels within the bone underlining the cartilage ( i.e . distal femoral epiphysis and proximal tibial epiphysis) of rats that have undergone an OA-induced surgery. (uwo.ca)
  • The tumor promoter, phorbol 12-myristate 13-acetate, and the diacylglycerol analog, 1-oleoyl-2-acetyl-sn-glycerol, caused the redistribution of protein kinase C activity to the membrane fraction in microvessels from immature rats. (elsevier.com)
  • 1989). However, AChE activity has not been studied biochemically in microvessels isolated from human brains. (springer.com)
  • Here we analyzed the intravasation of invasive human breast cancer cells within a tissue-engineered microvessel model of the tumor microenvironment. (aacrjournals.org)
  • The multi-layered microvessels combine polymers that are compatible with human cells, but also encourage cell growth and function. (medicaldesignbriefs.com)
  • Human cells of a specific type can be mixed with appropriately customized materials destined for each layer of the microvessel. (medicaldesignbriefs.com)
  • Expression of adhesion molecules, VAP-1 and CD44v3, in intra- and peritumoral microvessels of human skin melanoma. (aacrjournals.org)
  • Noncytolytic human lymphocytes injure dermal microvessels in t. (mysciencework.com)
  • Noncytolytic human lymphocytes injure dermal microvessels in the huPBL-SCID skin graft model. (mysciencework.com)
  • Nevertheless, human microvessel injury was similar between the two groups. (mysciencework.com)
  • Paraffin-embedded specimens from 47 cases of OSCC and 12 cases of normal mucosa were investigated immunohistochemically with anti-CD-31 antibody to stain microvessels and anti-tryptase antibody to visualize mast cells. (unboundmedicine.com)
  • ED induced the decrease of eNOS expression in the microvessels and the downregulation was attenuated in ED+ET_A group. (nii.ac.jp)
  • The grafts were harvested between day 16 and 21 after PBL injection, then the histology was scored by a blinded observer for degree of infiltration, microvessel injury, induction of epidermal MHC class II, and perforin expression. (mysciencework.com)
  • The microvessel technology will help to create new tissues and provide an important tool for evaluating new therapies. (medicaldesignbriefs.com)
  • As the body of knowledge in microvessel anatomy and physiology continues to expand, the scientific community has sought to develop accurate and reliable tools for reconstructing and analyzing properties of microvessels in healthy and abnormal tissues. (mbfbioscience.com)
  • Microvessel networks [corrected] pre-formed in artificial clinical grade dermal substitutes in vitro using cells from haematopoietic tissues. (ox.ac.uk)
  • Sections from autopsied hearts of diabetic patients with and without myocardial infarction as well as from those of patients with infarcts and no diabetes were used for morphometric studies of intramural microvessels in areas without infarction. (nih.gov)
  • The ability to measure ACE activity of the microvessels in vitro provides a unique opportunity to study the properties of the enzyme in intact cerebrovascular endothelial cells. (ahajournals.org)
  • Microvessels from Alzheimer's disease brains kill neurons in vitro. (semanticscholar.org)
  • Endothelial surface glycocalyx plays an important role in the regulation of microvessel permeability by possibly changing its charge and configuration. (unlv.edu)
  • 2002) for the interendothelial cleft with a negatively charged surface glycocalyx layer, to include the filtration due to hydrostatic and oncotic pressures across the microvessel wall, as well as the electrical potential across the surface fiber layer. (unlv.edu)
  • We describe a novel technique to visualize and dissect interactions of HPCs with BM microvessels in the skulls of anesthetized mice. (rupress.org)
  • Elevated oxidative stress, iron accumulation around microvessels and i" by Wei-Yi Ong, Andrew M. Jenner et al. (edu.au)
  • Results -15-F 2t -IsoP-mediated constriction of periventricular microvessels decreased as a function of age such that in the fetus it was ≈2.5-fold greater than in juvenile pigs. (ahajournals.org)
  • The samples were subjected to immunohistochemistry staining: double staining with CD31 and D2-40 for microvessels, or Ki-67 for the analysis of cell proliferation. (nih.gov)
  • the latter two are similar to microvessels found in systemic tumors. (elsevier.com)
  • Enhancement of platelet aggregation by tranylcypromine in mouse cerebral microvessels. (ahajournals.org)
  • We evaluated the association of several microvessel factors with overall survival, including the ratio of total microvessel perimeter to total tumor area (TP/TA), the tumor hypoxic ratio, and the ratio of total microvessel number to total tumor area (TN/TA). (aacrjournals.org)
  • In the present study, microvessel factors, including hotspot MVD and TP/TA, TN/TA, and hypoxic ratios, in biopsy specimens from 51 patients with esophageal cancer treated with chemoradiotherapy were analyzed, and the relations between these factors and overall survival were assessed. (aacrjournals.org)
  • Our previous study demonstrated that ROS release from fMLP-stimulated neutrophils increases microvessel permeability independently from leukocyte adhesion and migration. (cdc.gov)
  • However, the study of microvessels using CT has been exceptionally difficult due to their small ( (uwo.ca)
  • Results gained from this study will further provide information into the role that microvessels may play in OA. (uwo.ca)
  • His group plans to study the microvessels within heart valves. (usf.edu)
  • In this study, we examined protein kinase C in isolated cerebral microvessels. (naver.com)
  • A potentially BEC-specific immunohistochemical marker in rat tissue was found in a monoclonal antibody, designated JG12, that was raised against rat glomerular membrane protein fractions and labeled intensely microvessels of kidney and lung ( 7 ). (asnjournals.org)
  • Our results suggest that frog mesenteric tissue is much less selective to solutes than the microvessel wall. (asme.org)