The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
A form of interactive entertainment in which the player controls electronically generated images that appear on a video display screen. This includes video games played in the home on special machines or home computers, and those played in arcades.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.
Microscopy in which television cameras are used to brighten magnified images that are otherwise too dark to be seen with the naked eye. It is used frequently in TELEPATHOLOGY.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
Recording of visual and sometimes sound signals on magnetic tape.
A type of scanning probe microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. The vertical position is recorded as a spring attached to the probe rises and falls in response to peaks and valleys on the surface. These deflections produce a topographic map of the sample.
Electron microscopy in which the ELECTRONS or their reaction products that pass down through the specimen are imaged below the plane of the specimen.
Microscopy in which the samples are first stained immunocytochemically and then examined using an electron microscope. Immunoelectron microscopy is used extensively in diagnostic virology as part of very sensitive immunoassays.
Fluorescence microscopy utilizing multiple low-energy photons to produce the excitation event of the fluorophore. Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal confocal microscopes. Ultimately this allows spatial isolation of the excitation event, enabling deeper imaging into optically thick tissue, while restricting photobleaching and phototoxicity to the area being imaged.
Electron microscopy involving rapid freezing of the samples. The imaging of frozen-hydrated molecules and organelles permits the best possible resolution closest to the living state, free of chemical fixatives or stains.
A type of scanning probe microscopy in which a very sharp conducting needle is swept just a few angstroms above the surface of a sample. The tiny tunneling current that flows between the sample and the needle tip is measured, and from this are produced three-dimensional topographs. Due to the poor electron conductivity of most biological samples, thin metal coatings are deposited on the sample.
The science and application of a double-beam transmission interference microscope in which the illuminating light beam is split into two paths. One beam passes through the specimen while the other beam reflects off a reference mirror before joining and interfering with the other. The observed optical path difference between the two beams can be measured and used to discriminate minute differences in thickness and refraction of non-stained transparent specimens, such as living cells in culture.
Microscopy using polarized light in which phenomena due to the preferential orientation of optical properties with respect to the vibration plane of the polarized light are made visible and correlated parameters are made measurable.
A form of interference microscopy in which variations of the refracting index in the object are converted into variations of intensity in the image. This is achieved by the action of a phase plate.
A type of TRANSMISSION ELECTRON MICROSCOPY in which the object is examined directly by an extremely narrow electron beam scanning the specimen point-by-point and using the reactions of the electrons that are transmitted through the specimen to create the image. It should not be confused with SCANNING ELECTRON MICROSCOPY.
Scanning microscopy in which a very sharp probe is employed in close proximity to a surface, exploiting a particular surface-related property. When this property is local topography, the method is atomic force microscopy (MICROSCOPY, ATOMIC FORCE), and when it is local conductivity, the method is scanning tunneling microscopy (MICROSCOPY, SCANNING TUNNELING).
A technique of inputting two-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.
A scientific tool based on ULTRASONOGRAPHY and used not only for the observation of microstructure in metalwork but also in living tissue. In biomedical application, the acoustic propagation speed in normal and abnormal tissues can be quantified to distinguish their tissue elasticity and other properties.
Elements of limited time intervals, contributing to particular results or situations.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Communications via an interactive conference between two or more participants at different sites, using computer networks (COMPUTER COMMUNICATION NETWORKS) or other telecommunication links to transmit audio, video, and data.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
The process of generating three-dimensional images by electronic, photographic, or other methods. For example, three-dimensional images can be generated by assembling multiple tomographic images with the aid of a computer, while photographic 3-D images (HOLOGRAPHY) can be made by exposing film to the interference pattern created when two laser light sources shine on an object.
The transmission and reproduction of transient images of fixed or moving objects. An electronic system of transmitting such images together with sound over a wire or through space by apparatus that converts light and sound into electrical waves and reconverts them into visible light rays and audible sound. (From Webster, 3rd ed)
Established cell cultures that have the potential to propagate indefinitely.
Characteristics or attributes of the outer boundaries of objects, including molecules.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Methods of creating machines and devices.
Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
The art, technique, or business of producing motion pictures for entertainment, propaganda, or instruction.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Materials, frequently computer applications, that combine some or all of text, sound, graphics, animation, and video into integrated packages. (Thesaurus of ERIC Descriptors, 1994)
Auditory and visual instructional materials.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Preparation for electron microscopy of minute replicas of exposed surfaces of the cell which have been ruptured in the frozen state. The specimen is frozen, then cleaved under high vacuum at the same temperature. The exposed surface is shadowed with carbon and platinum and coated with carbon to obtain a carbon replica.
The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Slender, cylindrical filaments found in the cytoskeleton of plant and animal cells. They are composed of the protein TUBULIN and are influenced by TUBULIN MODULATORS.
Input/output devices designed to receive data in an environment associated with the job to be performed, and capable of transmitting entries to, and obtaining output from, the system of which it is a part. (Computer Dictionary, 4th ed.)
An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (LASER) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum.
Endoscopic surgical procedures performed with visualization via video transmission. When real-time video is combined interactively with prior CT scans or MRI images, this is called image-guided surgery (see SURGERY, COMPUTER-ASSISTED).
Relating to the size of solids.
Recording serial images of a process at regular intervals spaced out over a longer period of time than the time in which the recordings will be played back.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
The properties, processes, and behavior of biological systems under the action of mechanical forces.
A yellow metallic element with the atomic symbol Au, atomic number 79, and atomic weight 197. It is used in jewelry, goldplating of other metals, as currency, and in dental restoration. Many of its clinical applications, such as ANTIRHEUMATIC AGENTS, are in the form of its salts.
The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
The development and use of techniques to study physical phenomena and construct structures in the nanoscale size range or smaller.
Microscopy in which the image is formed by ultraviolet radiation and is displayed and recorded by means of photographic film.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Discrete concentrations of energy, apparently massless elementary particles, that move at the speed of light. They are the unit or quantum of electromagnetic radiation. Photons are emitted when electrons move from one energy state to another. (From Hawley's Condensed Chemical Dictionary, 11th ed)
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
A specialized field of physics and engineering involved in studying the behavior and properties of light and the technology of analyzing, generating, transmitting, and manipulating ELECTROMAGNETIC RADIATION in the visible, infrared, and ultraviolet range.
The technique of using a microtome to cut thin or ultrathin sections of tissues embedded in a supporting substance. The microtome is an instrument that hold a steel, glass or diamond knife in clamps at an angle to the blocks of prepared tissues, which it cuts in sections of equal thickness.
Transmission of information over distances via electronic means.
The technique of placing cells or tissue in a supporting medium so that thin sections can be cut using a microtome. The medium can be paraffin wax (PARAFFIN EMBEDDING) or plastics (PLASTIC EMBEDDING) such as epoxy resins.
Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The recording of images in three-dimensional form on a photographic film by exposing it to a laser beam reflected from the object under study.
Methods used to study CELLS.
The minute vessels that connect the arterioles and venules.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
The act, process, or result of passing from one place or position to another. It differs from LOCOMOTION in that locomotion is restricted to the passing of the whole body from one place to another, while movement encompasses both locomotion but also a change of the position of the whole body or any of its parts. Movement may be used with reference to humans, vertebrate and invertebrate animals, and microorganisms. Differentiate also from MOTOR ACTIVITY, movement associated with behavior.
Adherence of cells to surfaces or to other cells.
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
The technique of washing tissue specimens with a concentrated solution of a heavy metal salt and letting it dry. The specimen will be covered with a very thin layer of the metal salt, being excluded in areas where an adsorbed macromolecule is present. The macromolecules allow electrons from the beam of an electron microscope to pass much more readily than the heavy metal; thus, a reversed or negative image of the molecule is created.
Specific particles of membrane-bound organized living substances present in eukaryotic cells, such as the MITOCHONDRIA; the GOLGI APPARATUS; ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.
A family of 3,6-di(substituted-amino)-9-benzoate derivatives of xanthene that are used as dyes and as indicators for various metals; also used as fluorescent tracers in histochemistry.
Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
One or more layers of EPITHELIAL CELLS, supported by the basal lamina, which covers the inner or outer surfaces of the body.
The technique of using a cryostat or freezing microtome, in which the temperature is regulated to -20 degrees Celsius, to cut ultrathin frozen sections for microscopic (usually, electron microscopic) examination.
LIGHT, it's processes and properties, and the characteristics of materials interacting with it.
Photography of objects viewed under a microscope using ordinary photographic methods.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)
A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.
Transmission of live or pre-recorded audio or video content via connection or download from the INTERNET.
A self-learning technique, usually online, involving interaction of the student with programmed instructional materials.
Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.
The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed, e.g., a substance can be irradiated with invisible radiation and emit visible light. X-ray fluorescence is used in diagnosis.
The evaluation of incidents involving the loss of function of a device. These evaluations are used for a variety of purposes such as to determine the failure rates, the causes of failures, costs of failures, and the reliability and maintainability of devices.
Fibers composed of MICROFILAMENT PROTEINS, which are predominately ACTIN. They are the smallest of the cytoskeletal filaments.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.
The rate dynamics in chemical or physical systems.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Condensed areas of cellular material that may be bounded by a membrane.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Consultation via remote telecommunications, generally for the purpose of diagnosis or treatment of a patient at a site remote from the patient or primary physician.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Improvement of the quality of a picture by various techniques, including computer processing, digital filtering, echocardiographic techniques, light and ultrastructural MICROSCOPY, fluorescence spectrometry and microscopy, scintigraphy, and in vitro image processing at the molecular level.
A replica technique in which cells are frozen to a very low temperature and cracked with a knife blade to expose the interior surfaces of the cells or cell membranes. The cracked cell surfaces are then freeze-dried to expose their constituents. The surfaces are now ready for shadowing to be viewed using an electron microscope. This method differs from freeze-fracturing in that no cryoprotectant is used and, thus, allows for the sublimation of water during the freeze-drying process to etch the surfaces.
Non-invasive, endoscopic imaging by use of VIDEO CAPSULE ENDOSCOPES to perform examination of the gastrointestinal tract, especially the small bowel.
Delivery of health services via remote telecommunications. This includes interactive consultative and diagnostic services.
Materials which have structured components with at least one dimension in the range of 1 to 100 nanometers. These include NANOCOMPOSITES; NANOPARTICLES; NANOTUBES; and NANOWIRES.
Pieces of glass or other transparent materials used for magnification or increased visual acuity.
Methods of preparing tissue for examination and study of the origin, structure, function, or pathology.
Systematic identification, development, organization, or utilization of educational resources and the management of these processes. It is occasionally used also in a more limited sense to describe the use of equipment-oriented techniques or audiovisual aids in educational settings. (Thesaurus of ERIC Descriptors, December 1993, p132)
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
The minute vessels that collect blood from the capillary plexuses and join together to form veins.
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS.
A tomographic technique for obtaining 3-dimensional images with transmission electron microscopy.
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
Any of the numerous types of clay which contain varying proportions of Al2O3 and SiO2. They are made synthetically by heating aluminum fluoride at 1000-2000 degrees C with silica and water vapor. (From Hawley's Condensed Chemical Dictionary, 11th ed)
Identification and measurement of ELEMENTS and their location based on the fact that X-RAYS emitted by an element excited by an electron beam have a wavelength characteristic of that element and an intensity related to its concentration. It is performed with an electron microscope fitted with an x-ray spectrometer, in scanning or transmission mode.
A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.
The process of converting analog data such as continually measured voltage to discrete, digital form.
Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).
The study of PHYSICAL PHENOMENA and PHYSICAL PROCESSES as applied to living things.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Proteins found in any species of bacterium.
A microtubule subunit protein found in large quantities in mammalian brain. It has also been isolated from SPERM FLAGELLUM; CILIA; and other sources. Structurally, the protein is a dimer with a molecular weight of approximately 120,000 and a sedimentation coefficient of 5.8S. It binds to COLCHICINE; VINCRISTINE; and VINBLASTINE.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
A tissue preparation technique that involves the injecting of plastic (acrylates) into blood vessels or other hollow viscera and treating the tissue with a caustic substance. This results in a negative copy or a solid replica of the enclosed space of the tissue that is ready for viewing under a scanning electron microscope.
Behavior of LIGHT and its interactions with itself and materials.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
An analytical transmission electron microscopy method using an electron microscope fitted with an energy filtering lens. The method is based on the principle that some of the ELECTRONS passing through the specimen will lose energy when they ionize inner shell electrons of the atoms in the specimen. The amount of energy loss is dependent upon the element. Analysis of the energy loss spectrum (ELECTRON ENERGY-LOSS SPECTROSCOPY) reveals the elemental composition of a specimen. It is used analytically and quantitatively to determine which, how much of, and where specific ELEMENTS are in a sample. For example, it is used for elemental mapping of PHOSPHORUS to trace the strands of NUCLEIC ACIDS in nucleoprotein complexes.
A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Analysis of the intensity of Raman scattering of monochromatic light as a function of frequency of the scattered light.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
The mimicking of the behavior of one individual by another.
A family of spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one derivatives. These are used as dyes, as indicators for various metals, and as fluorescent labels in immunoassays.
The use of molecularly targeted imaging probes to localize and/or monitor biochemical and cellular processes via various imaging modalities that include RADIONUCLIDE IMAGING; ULTRASONOGRAPHY; MAGNETIC RESONANCE IMAGING; FLUORESCENCE IMAGING; and MICROSCOPY.
Methods developed to aid in the interpretation of ultrasound, radiographic images, etc., for diagnosis of disease.
Endoscopes for examining the interior of the larynx.
Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)
The educational process of instructing.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Analog or digital communications device in which the user has a wireless connection from a telephone to a nearby transmitter. It is termed cellular because the service area is divided into multiple "cells." As the user moves from one cell area to another, the call is transferred to the local transmitter.
That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range.
The diversion of RADIATION (thermal, electromagnetic, or nuclear) from its original path as a result of interactions or collisions with atoms, molecules, or larger particles in the atmosphere or other media. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Antibodies produced by a single clone of cells.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
The quantity of volume or surface area of CELLS.
Light-induced change in a chromophore, resulting in the loss of its absorption of light of a particular wave length. The photon energy causes a conformational change in the photoreceptor proteins affecting PHOTOTRANSDUCTION. This occurs naturally in the retina (ADAPTATION, OCULAR) on long exposure to bright light. Photobleaching presents problems when occurring in PHOTODYNAMIC THERAPY, and in FLUORESCENCE MICROSCOPY. On the other hand, this phenomenon is exploited in the technique, FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING, allowing measurement of the movements of proteins and LIPIDS in the CELL MEMBRANE.
Liquids transforming into solids by the removal of heat.
The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.
Computer disks storing data with a maximum reduction of space and bandwidth. The compact size reduces cost of transmission and storage.
The study of parasites and PARASITIC DISEASES.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.
Platforms that provide the ability and tools to create and publish information accessed via the INTERNET. Generally these platforms have three characteristics with content user generated, high degree of interaction between creator and viewer, and easily integrated with other sites.
Small uniformly-sized spherical particles, of micrometer dimensions, frequently labeled with radioisotopes or various reagents acting as tags or markers.
Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)
A type of FLUORESCENCE SPECTROSCOPY using two FLUORESCENT DYES with overlapping emission and absorption spectra, which is used to indicate proximity of labeled molecules. This technique is useful for studying interactions of molecules and PROTEIN FOLDING.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Information application based on a variety of coding methods to minimize the amount of data to be stored, retrieved, or transmitted. Data compression can be applied to various forms of data, such as images and signals. It is used to reduce costs and increase efficiency in the maintenance of large volumes of data.
Nanoparticles produced from metals whose uses include biosensors, optics, and catalysts. In biomedical applications the particles frequently involve the noble metals, especially gold and silver.
The portion of an interactive computer program that issues messages to and receives commands from a user.
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Education via communication media (correspondence, radio, television, computer networks) with little or no in-person face-to-face contact between students and teachers. (ERIC Thesaurus, 1997)
A purely physical condition which exists within any material because of strain or deformation by external forces or by non-uniform thermal expansion; expressed quantitatively in units of force per unit area.
The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The number of CELLS of a specific kind, usually measured per unit volume or area of sample.
Compounds that contain three methine groups. They are frequently used as cationic dyes used for differential staining of biological materials.
The physical characteristics and processes of biological systems.
A cell line derived from cultured tumor cells.
A scanning probe microscopy technique that uses an ultramicroelectrode as the scanning probe that simultaneously records changes in electrochemical potential as it scans thereby creating topographical images with localized electrochemical information.
Encrustations, formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedding in extracellular polymers, that adhere to surfaces such as teeth (DENTAL DEPOSITS); PROSTHESES AND IMPLANTS; and catheters. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and antifouling agents.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
The circulation of the BLOOD through the MICROVASCULAR NETWORK.
Artificial, single or multilaminar vesicles (made from lecithins or other lipids) that are used for the delivery of a variety of biological molecules or molecular complexes to cells, for example, drug delivery and gene transfer. They are also used to study membranes and membrane proteins.
The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.
The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
Resistance and recovery from distortion of shape.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The adopting or performing the role of another significant individual in order to gain insight into the behavior of that person.
An organization of cells into an organ-like structure. Organoids can be generated in culture. They are also found in certain neoplasms.
Rhythmic and patterned body movements which are usually performed to music.
The tendency of a gas or solute to pass from a point of higher pressure or concentration to a point of lower pressure or concentration and to distribute itself throughout the available space. Diffusion, especially FACILITATED DIFFUSION, is a major mechanism of BIOLOGICAL TRANSPORT.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
A group of glucose polymers made by certain bacteria. Dextrans are used therapeutically as plasma volume expanders and anticoagulants. They are also commonly used in biological experimentation and in industry for a wide variety of purposes.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Method of making images on a sensitized surface by exposure to light or other radiant energy.
Any spaces or cavities within a cell. They may function in digestion, storage, secretion, or excretion.
One of the protein CROSS-LINKING REAGENTS that is used as a disinfectant for sterilization of heat-sensitive equipment and as a laboratory reagent, especially as a fixative.
Systems composed of a computer or computers, peripheral equipment, such as disks, printers, and terminals, and telecommunications capabilities.

The small GTPase RalA targets filamin to induce filopodia. (1/1875)

The Ras-related small GTPases Rac, Rho, Cdc42, and RalA bind filamin, an actin filament-crosslinking protein that also links membrane and other intracellular proteins to actin. Of these GTPases only RalA binds filamin in a GTP-specific manner, and GTP-RalA elicits actin-rich filopods on surfaces of Swiss 3T3 cells and recruits filamin into the filopodial cytoskeleton. Either a dominant negative RalA construct or the RalA-binding domain of filamin 1 specifically block Cdc42-induced filopod formation, but a Cdc42 inhibitor does not impair RalA's effects, which, unlike Cdc42, are Rac independent. RalA does not generate filopodia in filamin-deficient human melanoma cells, whereas transfection of filamin 1 restores the functional response. RalA therefore is a downstream intermediate in Cdc42-mediated filopod production and uses filamin in this pathway.  (+info)

Isosmotic modulation of Ca2+-regulated exocytosis in guinea-pig antral mucous cells: role of cell volume. (2/1875)

1. Exocytotic events and changes of cell volume in mucous cells from guinea-pig antrum were examined by video-enhanced optical microscopy. 2. Acetylcholine (ACh) evoked exocytotic events following cell shrinkage, the frequency and extent of which depended on the ACh concentration. ACh actions were mimicked by ionomycin and thapsigargin, and inhibited by Ca2+-free solution and Ca2+ channel blockers (Ni2+, Cd2+ and nifedipine). Application of 100 microM W-7, a calmodulin inhibitor, also inhibited the ACh-induced exocytotic events. These results indicate that ACh actions are mediated by intracellular Ca2+ concentration ([Ca2+]i) in antral mucous cells. 3. The effects of ion channel blockers on exocytotic events and cell shrinkage evoked by ACh were examined. Inhibition of KCl release (quinine, Ba2+, NPPB or KCl solution) suppressed both the exocytotic events and cell shrinkage evoked by ACh. 4. Bumetanide (inhibition of NaCl entry) or Cl--free solution (increasing Cl- release and inhibition of NaCl entry) evoked exocytotic events following cell shrinkage in unstimulated antral mucous cells and caused further cell shrinkage and increases in the frequency of exocytotic events in ACh-stimulated cells. However, Cl--free solution did not evoke exocytotic events in unstimulated cells in the absence of extracellular Ca2+, although cell shrinkage occurred. 5. To examine the effects of cell volume on ACh-evoked exocytosis, the cell volume was altered by increasing the extracellular K+ concentration. The results showed that cell shrinkage increases the frequency of ACh-evoked exocytotic events and cell swelling decreases them. 6. Osmotic shrinkage or swelling caused the frequency of ACh-evoked exocytotic events to increase. This suggests that the effects of cell volume on ACh-evoked exocytosis under anisosmotic conditions may not be the same as those under isosmotic conditions. 7. In antral mucous cells, Ca2+-regulated exocytosis is modulated by cell shrinkage under isosmotic conditions.  (+info)

Spread of vasodilatation and vasoconstriction along feed arteries and arterioles of hamster skeletal muscle. (3/1875)

1. In arterioles of the hamster cheek pouch, vasodilatation and vasoconstriction can spread via the conduction of electrical signals through gap junctions between cells that comprise the vessel wall. However, conduction in resistance networks supplying other tissues has received relatively little attention. In anaesthetized hamsters, we have investigated the spread of dilatation and constriction along feed arteries and arterioles of the retractor muscle, which is contiguous with the cheek pouch. 2. When released from a micropipette, acetylcholine (ACh) triggered vasodilatation that spread rapidly along feed arteries external to the muscle and arterioles within the muscle. Responses were independent of changes in wall shear rate, perivascular nerve activity, or release of nitric oxide, indicating cell-to-cell conduction. 3. Vasodilatation conducted without decrement along unbranched feed arteries, yet decayed markedly in arteriolar networks. Thus, branching of the conduction pathway dissipated the vasodilatation. 4. Noradrenaline (NA) or a depolarizing KCl stimulus evoked constriction of arterioles and feed arteries of the retractor muscle that was constrained to the vicinity of the micropipette. This behaviour contrasts sharply with the conduction of vasodilatation in these microvessels and with the conduction of vasoconstriction elicited by NA and KCl in cheek pouch arterioles. 5. Focal electrical stimulation produced constriction that spread rapidly along feed arteries and arterioles. These responses were inhibited by tetrodotoxin or prazosin, confirming the release of NA along perivascular sympathetic nerves, which are absent from arterioles studied in the cheek pouch. Thus, sympathetic nerve activity co-ordinated the contraction of smooth muscle cells as effectively as the conduction of vasodilatation co-ordinated their relaxation. 6. In the light of previous findings in the cheek pouch, the properties of vasoconstriction and vasodilatation in feed arteries and arterioles of the retractor muscle indicate that substantive differences can exist in the nature of signal transmission along microvessels of tissues that differ in structure and function.  (+info)

Single-polymer dynamics in steady shear flow. (4/1875)

The conformational dynamics of individual, flexible polymers in steady shear flow were directly observed by the use of video fluorescence microscopy. The probability distribution for the molecular extension was determined as a function of shear rate, gamma;, for two different polymer relaxation times, tau. In contrast to the behavior in pure elongational flow, the average polymer extension in shear flow does not display a sharp coil-stretch transition. Large, aperiodic temporal fluctuations were observed, consistent with end-over-end tumbling of the molecule. The rate of these fluctuations (relative to the relaxation rate) increased as the Weissenberg number, gamma;tau, was increased.  (+info)

In vivo blood flow abnormalities in the transgenic knockout sickle cell mouse. (5/1875)

The accepted importance of circulatory impairment to sickle cell anemia remains to be verified by in vivo experimentation. Intravital microscopy studies of blood flow in patients are limited to circulations that can be viewed noninvasively and are restricted from deliberate perturbations of the circulation. Further knowledge of sickle blood flow abnormalities has awaited an animal model of human sickle cell disease. We compared blood flow in the mucosal-intestinal microvessels of normal mice with that in transgenic knockout sickle cell mice that have erythrocytes containing only human hemoglobin S and that exhibit a degree of hemolytic anemia and pathological complications similar to the human disease. In sickle cell mice, in addition to seeing blood flow abnormalities such as sludging in all microvessels, we detected decreased blood flow velocity in venules of all diameters. Flow responses to hyperoxia in both normal and sickle cell mice were dramatic, but opposite: Hyperoxia promptly slowed or halted flow in normal mice but markedly enhanced flow in sickle cell mice. Intravital microscopic studies of this murine model provide important insights into sickle cell blood flow abnormalities and suggest that this model can be used to evaluate the causes of abnormal flow and new approaches to therapy of sickle cell disease.  (+info)

Locomotory behaviour of epitheliocytes and fibroblasts on metallic grids. (6/1875)

Behaviour of epitheliocytes and fibroblasts on special discontinuous substrata (metallic grids with square openings of 45x45 microm2) was examined in order to compare the ability of these cells to spread in two mutually perpendicular directions and to stretch over the void spaces. Two cell types with typical fibroblastic morphology, the AGO 1523 line of human foreskin fibroblasts and secondary cultures of mouse embryo fibroblasts, and three cell types with typical epithelial morphology, primary mouse hepatocytes, the IAR-2 line of rat liver cells and the MDCK line of canine kidney epithelial cells (clone 20) were used. We also examined the epitheliocytes (MDCK cells, clone 20) transformed to fibroblast-like morphology by treatment with hepatocyte growth factor/scatter factor (HGF/SF). Time-lapse video microscopy, scanning electron microscopy and immunofluorescence microscopy were used to examine cell reorganizations at various stages of spreading. It was found that early stages of spreading of fibroblasts and epitheliocytes were similar: the cell spread along two bars, perpendicular to each other (bar and crossbar), with the formation of a small triangular lamellar cytoplasm stretched over the opening. Later central parts of the bodies of the fibroblasts retracted from the bars so that the cells remained attached only by their polar lamellae. Successive expansions and partial retractions of these lamellae led to elongation of the cell body crossing several openings of the grid. Epitheliocytes, in contrast to fibroblasts, at the late stages of spreading did not retract their bodies and did not contract polar lamellae. As a result, their central lamellae stretched progressively over the openings. As a result of the treatment of MDCK epitheliocytes with HGF/SF the behaviour of the cells on the grids became similar to that of fibroblasts. It is suggested that these distinct spreading patterns of epitheliocytes and fibroblasts are due to the type-specific differences in the actin-myosin cortex. Experiments with microtubule-specific drugs, colcemid and taxol, indicate that the organization of this cortex is under microtubular control.  (+info)

Transmembrane calcium influx induced by ac electric fields. (7/1875)

Exogenous electric fields induce cellular responses including redistribution of integral membrane proteins, reorganization of microfilament structures, and changes in intracellular calcium ion concentration ([Ca2+]i). Although increases in [Ca2+]i caused by application of direct current electric fields have been documented, quantitative measurements of the effects of alternating current (ac) electric fields on [Ca2+]i are lacking and the Ca2+ pathways that mediate such effects remain to be identified. Using epifluorescence microscopy, we have examined in a model cell type the [Ca2+]i response to ac electric fields. Application of a 1 or 10 Hz electric field to human hepatoma (Hep3B) cells induces a fourfold increase in [Ca2+]i (from 50 nM to 200 nM) within 30 min of continuous field exposure. Depletion of Ca2+ in the extracellular medium prevents the electric field-induced increase in [Ca2+]i, suggesting that Ca2+ influx across the plasma membrane is responsible for the [Ca2+]i increase. Incubation of cells with the phospholipase C inhibitor U73122 does not inhibit ac electric field-induced increases in [Ca2+]i, suggesting that receptor-regulated release of intracellular Ca2+ is not important for this effect. Treatment of cells with either the stretch-activated cation channel inhibitor GdCl3 or the nonspecific calcium channel blocker CoCl2 partially inhibits the [Ca2+]i increase induced by ac electric fields, and concomitant treatment with both GdCl3 and CoCl2 completely inhibits the field-induced [Ca2+]i increase. Since neither Gd3+ nor Co2+ is efficiently transported across the plasma membrane, these data suggest that the increase in [Ca2+]i induced by ac electric fields depends entirely on Ca2+ influx from the extracellular medium.  (+info)

Vesicle deformation by an axial load: from elongated shapes to tethered vesicles. (8/1875)

A sufficiently large force acting on a single point of the fluid membrane of a flaccid phospholipid vesicle is known to cause the formation of a narrow bilayer tube (tether). We analyze this phenomenon by means of general mathematical methods allowing us to determine the shapes of strongly deformed vesicles including their stability. Starting from a free vesicle with an axisymmetric, prolate equilibrium shape, we consider an axial load that pulls (or pushes) the poles of the vesicle apart. Arranging the resulting shapes of strained vesicles in dependence of the axial deformation and of the area difference of monolayers, phase diagrams of stable shapes are presented comprising prolate shapes with or without equatorial mirror symmetry. For realistic values of membrane parameters, we study the force-extension relation of strained vesicles, and we demonstrate in detail how the initially elongated shape of an axially stretched vesicle transforms into a shape involving a membrane tether. This tethering transition may be continuous or discontinuous. If the free vesicle is mirror symmetric, the mirror symmetry is broken as the tether forms. The stability analysis of tethered shapes reveals that, for the considered vesicles, the stable shape is always asymmetric (polar), i.e., it involves only a single tether on one side of the main vesicle body. Although a bilayer tube formed from a closed vesicle is not an ideal cylinder, we show that, for most practical purposes, it is safe to assume a cylindrical geometry of tethers. This analysis is supplemented by the documentation of a prototype experiment supporting our theoretical predictions. It shows that the currently accepted model for the description of lipid-bilayer elasticity (generalized bilayer couple model) properly accounts for the tethering phenomenon.  (+info)

I defended my PhD of Bioinformatics in December 2015 (CBIO, Mines ParisTech), after three years under the supervision of Thomas Walter, and the direction of Robert Barouki and Jean-Philippe Vert. During my PhD, I was interested in applying computer vision and machine learning methods to video-microscopy data. My research focused on the development of tools for automatic and quantitative characterization of cellular dynamic processes from video-microscopy data, following chemical exposure ...
The migration of lymphocytes through different lymphoid organs and other tissues of the body is a carefully controlled process that enables immune cells to encounter the cognate antigen and to disseminate memory and effector cells for immunologic surveillance (7). In this study, we have demonstrated, using intravital video microscopy, that activation of T lymphocytes by ConA leads to profound alterations in the sequential migration of these cells in the microcirculation of different lymphoid and nonlymphoid organs. The activated T cells either lose or exhibit a diminished capacity to enter Peyers patches and the spleen, whereas they exhibit a preferential distribution to the liver. A similar behavior of activated lymphocytes in lymph nodes or Peyers patches has been reported in previous studies, although these were in vivo homing studies that used51Cr-labeled lymphocytes or an in vitro binding assay of frozen sections (8, 13, 14). Our study also clearly demonstrates that stimulation of T ...
Targeting the dynamic tumor immune microenvironment (TIME) can provide effective therapeutic strategies for cancer. Neutrophils are the predominant leukocyte population in mice and humans, and mounting evidence implicates these cells during tumor growth and metastasis. Neutrophil extracellular traps (NETs) are networks of extracellular neutrophil DNA fibers that are capable of binding tumor cells to support metastatic progression. Here, we demonstrate that circulating NET levels are elevated in advanced esophageal, gastric, and lung cancer patients compared with local cancers and healthy controls. Using preclinical murine models of lung and colon cancer, in combination with intravital video microscopy, we show that NETs functionally regulate disease progression and that blocking NETosis through multiple strategies significantly inhibits spontaneous metastasis to the lung and liver. Furthermore, we show how inhibiting tumor-induced NETs decreases cancer cell adhesion to liver sinusoids following ...
Cytotoxic CD8(+) T cells are considered important effector cells contributing to neuronal damage in inflammatory and degenerative CNS disorders. Using time-lapse video microscopy and two-photon imaging in combination with whole-cell patch-clamp recordings, we here show that major histocompatibility class I (MHC I)-restricted neuronal antigen presentation and T cell receptor specificity determine CD8(+) T-cell locomotion and neuronal damage in culture and hippocampal brain slices. Two separate functional consequences result from a direct cell-cell contact between antigen-presenting neurons and antigen-specific CD8(+) T cells. (1) An immediate impairment of electrical signaling in single neurons and neuronal networks occurs as a result of massive shunting of the membrane capacitance after insertion of channel-forming perforin (and probably activation of other transmembrane conductances), which is paralleled by an increase of intracellular Ca(2+) levels (within ,10 min). (2) Antigen-dependent ...
We apply three-dimensional confocal microscopy and time lapse video-microscopy techniques to visualize molecular dynamics at the immunological synapse (IS). The major contribution of our research team to the field during the last few years has been to contribute to define the biological function of the IS. We propose that the IS has no specific function in T cell activation, on the contrary it is the manifestation of the inter-cellular communication occurring during T cell/APC cognate interactions. Our results show that: i) the large-scale molecular clustering and segregation characteristic of a mature IS is not required for productive TCR triggering and for T cell activation (indeed some responses such as cytotoxicity can occur in the absence of mature IS formation); ii) T cells form different types of synapses depending on the strength and quality of antigenic stimulation; iii) synapses can be interrupted and re-formed while T cells add-up the interrupted signals; iv) synapses are not static ...
Concentrated suspensions of Brownian hard-spheres in water are an epitome for understandingthe glassy dynamics of both soft materials and supercooled molecular liquids.From an experimental point of view, such systems are especially suited to perform particle tracking easily, and,therefore, are a benchmark for novel optical techniques, applicable when primary particles cannot be resolved.Differential variance analysis (DVA) is one such novel technique thatsimplifies significantly the characterization of structural relaxation processes of soft glassy materials, since it isdirectly applicable to digital image sequences of the sample.DVA succeeds in monitoring not only the average dynamics, but also its spatio-temporal fluctuations, known as dynamic heterogeneities.In this work, we study the dynamics of dense suspensions of Brownian beads in water, imaged through digital video-microscopy,by using both DVA and single-particle tracking.We focus on two commonly used signatures of dynamic heterogeneities:the
A robust protocol to monitor neural populations by time-lapse video-microscopy followed by software-based post-processing is described. ...
Pittsburgh WHIRL Weddings December 2017: Kelsey Guth + Austin Eckenroth; Ali Giglio + Tom Simcho; Olivia Cimba + Matthew Recker; Sherrie Dunlap + Sean Gallagher; Kristi Rosiak + Joshua Billiel.. ...
To use the proscope with a biological microscope, first remove the lens by gently pressing down on the lens-release button and then rotating and removing the lens. Replace the lens with the threaded ring adapter, and then thread the sleeve into the adapter ring ...
Perineural invasion of cancer cells (CPNI) is found in most patients with pancreatic adenocarcinomas (PDA), prostate, or head and neck cancers. These patients undergo palliative rather than curative treatment due to dissemination of cancer along nerves, well beyond the extent of any local invasion. Although CPNI is a common source of distant tumor spread and a cause of significant morbidity, its exact mechanism is undefined. Immunohistochemical analysis of specimens excised from patients with PDAs showed a significant increase in the number of endoneurial macrophages (EMΦ) that lie around nerves invaded by cancer compared with normal nerves. Video microscopy and time-lapse analysis revealed that EMΦs are recruited by the tumor cells in response to colony-stimulated factor-1 secreted by invading cancer cells. Conditioned medium (CM) of tumor-activated EMΦs (tEMΦ) induced a 5-fold increase in migration of PDA cells compared with controls. Compared with resting EMΦs, tEMΦs secreted higher ...
From annual benefits, like Pittsburghs 50 Finest Gala, to concerts and shows, our Semi-Annual Event Guide is here to fill your calendar through January! Save the dates with Editor in Chief Rachel Jones on todays Dish on WISH 99.7.. ...
A whirl of bone and gore batters nearby enemies every 1 sec, dealing 16,629 Shadow damage every 1 sec, and healing you for 2% of your maximum health every time it deals damage. Lasts 1 sec per 10 Runic Power spent ...
We prepare you physically and psychologically for your upcoming procedure and can help limit some of the negative consequences that can occur during the time lapse between diagnosis and procedure.
in all dimensions. The techniques time resolution is limited only by the frame rate of the video camera. Rayleigh-Sommerfeld back-propagation has the advantage of providing a model-free approach to reconstructing the light field scattered by microscopic objects, and thus lends itself to high-speed processing and imaging. Holographic video microscopy thus can provide real-time feedback for three-dimensional micromanipulation of nanowires and nanorods. It also is useful for studying the rotational and translational motions of nanorods subjected to external forces. The present study takes advantage of the comparative simplicity of single isolated nanorods diffusion when viewed in the co-oriented frame of reference. This advantage is lost when studying the coupled motions of multiple nanorods, so that measurements of nanorods hydrodynamic and electrostatic interactions will be substantially more challenging than corresponding measurements on colloidal spheres. This complexity, however, arises ...
TY - JOUR. T1 - Live cell video microscopy of phagocytosis of fungal pathogens.. AU - Lewis, Leanne Elisabeth. AU - Bain, Judith Margaret. AU - Okai, Blessing. AU - Gow, Neil Andrew Robert. AU - Erwig, Lars-Peter. PY - 2013. Y1 - 2013. N2 - Phagocytic clearance of fungal pathogens, and microorganisms more generally, may be considered to consist of four distinct stages: (i) migration of phagocytes to the site where pathogens are located; (ii) recognition of pathogen-associated molecular patterns (PAMPs) through pattern recognition receptors (PRRs); (iii) engulfment of microorganisms bound to the phagocyte cell membrane, and (iv) processing of engulfed cells within maturing phagosomes and digestion of the ingested particle. Studies that assess phagocytosis in its entirety are informative but are limited in that they do not normally break the process down into migration, engulfment and phagosome maturation, which may be affected differentially. Furthermore, such studies assess uptake as a single ...
Rachel Finks current research deals with the cytoskeletal dynamics in living fish embryos, with a focus on how microfilament and microtubule behavior relate to cell arrangements during embryogenesis. In addition to her work on fish embryos, Fink also develops research video footage available for teaching and has published two compilations of movies: A Dozen Eggs: Time-Lapse Microscopy of Normal Development (Society for Developmental Biology and Sinauer Associates) and CELLebration (American Society for Cell Biology and Sinauer Associates).. Fink is known for her interactive courses, using everything from colorful chalk to research videos and student presentations to engage students in the material. Of her video footage, Fink notes, As an embryo develops from a fertilized egg, cells are constantly dividing, migrating, differentiating, and rearranging. Time-lapse video microscopy reveals these movements and allows analysis of the cellular mechanisms driving these transformations. Finks courses ...
IVF World News, Science Updates, IVF Jobs and Embryology Resumes. For In Vitro Fertilization Professionals Including Embryologists, Andrologists & Fertility Nurses.
We demonstrate complementary differences in the behavior of B lymphoblastoid cells adhering to LFA-1 or its counter-receptor ICAM-1. The interaction of B lymphoblastoid cells with glass-supported planar bilayers bearing LFA-1 or ICAM-1 was observed by time-lapse video microscopy, and the distribution of adhesion receptors on cells interacting with the planar bilayers was studied by immunofluorescence microscopy. B lymphoblasts formed a large contact area and crawled rapidly (up to 25 microns/min) on planar bilayers bearing ICAM-1. In contrast, these cells attached to planar bilayers bearing LFA-1 through a fixed point about which the cells actively pivoted, using a single stalk-like projection. Phorbol ester-stimulated lymphoblasts, which adhere more strongly to ICAM-1-bearing substrates than unstimulated lymphoblasts, were still capable of locomotion on ICAM-1. Phorbol ester stimulation of B lymphoblasts on planar bilayers bearing LFA-1 promoted a rapid conversion from stalk attachment to symmetrical
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Techniques to photograph or image skin photodamage have reached new levels of sophistication. This survey discusses clinical grading, light imaging techniques, video-microscopy and three-dimensional in vivo measuring systems.
Its a standing joke that creationists demand a complete time-lapse recording of evolution before theyre going to believe it. Joke no more: weve got one. Its a movie of bacteria evolving antibiotic resistance. I dont even need to explain it, because the video explains everything thats going on.
Lixin Liu is the author of this article in the Journal of Visualized Experiments: Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy
FLOW 800 is a unique fluorescence application enabling visual analysis of vascular blood flow dynamics. It compiles INFRARED 800 video sequences into visual maps, diagrams or side-by-side images, enabling an in-depth interpretation of fluorescence videos.
Have you seen the video cover from CrystEngComm? Illustrating the work published in their CrystEngComm article, Bergström et al. provide us with an overview of the growth process of self-assembled mesocrystals and ordered arrays from iron oxide nanocubes. By using visible light video microscopy, the authors were able to follow the movement of the growth crystal growth front over several minutes, capturing the transition from symmetrical coffee-ring deposition to dendritic or finger-like growth. The article forms part of the Nanocrystal growth via oriented attachment themed issue which is available here. In a first for both CrystEngComm and the Royal Society of Chemistry, a short video clip of the crystal growth has been embedded into the journal cover which you can access online here. To view the video, simply click on the main cover image and watch the cover come to life!* Adobe Acrobat 9 or higher is required ( If the video does not play in your browser, ...
Time Lapse Imaging is a technology also enables frequent monitoring of the progress and development of embryos without exposing them to extraneous elements. This technique covers important parameter like morphokinetic timings, fertilization, compaction etc.
Human neutrophils (white blood cells) are attracted to a chemotactic agent in this time-lapse video. This is one of many videos available for the classroom or broadcast.
Thanks to intravital microscopy, an optical imaging technique, Weigert and colleagues were able to determine for the first time how exocytosis actually occurs in the salivary glands of a living mouse.
An around-the-world time lapse journey celebrating our Sacred Earth. Six years in the making... seven continents... 24 countries. Photographed &a
Symptom of High Vibration Cause Test or Check Correction subsynchronous a. rubs caused by seals and bearings a. seen as whole fractions (1/2, 1/3) on real time a relieve rub components of running analyzer speed b. oil whirl b. typically 40% to 45% of running speed b. re-design bearing c. oil whip c. a whirl exciting a resonance c. re-design bearing. Get Price → ...
Techniques for generation of novel time-lapse videos that address the inherent sampling issues of traditional photographic time-lapse capture with the use of non-uniform sampling and non-linear filtering ...
Working on observations of bacteria going undercover in ways that might trick the human immune system, Stanford bioengineers have created a time-lapse video that shows this process step by step.
By Dave Andrusko No secret, I am a pushover when it comes to time-lapse videos that document a babys development from the very early stages to delivery-and beyond. I watch and re-watch them over and over. For example, just this morning, I revisited a 1:32 video-A Photo Every Pregnancy Day for 9 Months-at There ...
Using filters that only pass light when excited hydrogen atoms change state, pro photographer Cory Poole stacked 700 frames to uncover prominences and other disturbances of the Suns chromosphere. Learn how he did it at
The time-lapse imaging system avoids the need to move embryos or unnecessarily expose them to light from conventional examination.
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Kinetics of compaction on single DNA molecules are studied by fluorescence videomicroscopy in the presence of 1), Xenopus egg extracts and 2), purified nucleosome reconstitution systems using a combination of histones with either the histone chaperone Nucleosome Assembly Protein (NAP-1) or negatively charged macromolecules such as polyglutamic acid and RNA. The comparison shows that the compaction rates can differ by a factor of up to 1000 for the same amount of histones, depending on the system used and on the presence of histone tails, which can be subjected to post-translational modifications. Reactions with purified reconstitution systems follow a slow and sequential mechanism, compatible with the deposition of one (H3-H4)(2) tetramer followed by two (H2A-H2B) dimers. Addition of the histone chaperone NAP-1 increases both the rate of the reaction and the packing ratio of the final product. These stimulatory effects cannot be obtained with polyglutamic acid or RNA, suggesting that yNAP-1 ...
A differential interference contrast (DIC) determination device and method utilizes an illumination source, a layer having a pair of two apertures that receive illumination from the illumination source, and a photodetector to receive Youngs interference from the illumination passing through the pair of two apertures. In addition, a surface wave assisted optofluidic microscope and method utilize an illumination source, a fluid channel having a layer with at least one aperture as a surface, and a photodetector that receives a signal based on the illumination passing through the aperture. The layer is corrugated (e.g., via fabrication) and parameters of the corrugation optimize the signal received on the photodetector.
Poly(ADP-ribosyl)ation of proteins is an important switch in cellular processes including differentiation. In particular, recent advances reveal emerging role in differentiation of cancer cells. Here we described the effect of poly(ADP-ribose) polymerase inhibitor 3-aminobenzamide on a morphological feature of differentiation in transformed endothelial cells GM7373. Exposed to this drug, the cells displayed a 40% growth inhibition due to apoptosis, valued by time-lapse videomicroscopy and TUNEL assay. At the same time, cells showed enhanced motility and, when cultured on Matrigel enriched with FGF2 plus 3-aminobenzamide, began to organize a capillary network, in an Akt phosphorylation dependent manner, accompanied by a cyclooxygenase-2 induction. These results validate the role of poly(ADP-ribose) polymerase activity in cell differentiation and confirm the hypothesis that PARP may be a useful target for anticancer drugs.. ...
This webpage contains Thorlabs selection of components for performing differential interference contrast (DIC) imaging in DIY Cerna systems. DIC imaging is an extremely popular method for obtaining contrast from thin unlabeled samples in vitro.When mounted to a Cerna microscope body using the WFA01
The present work investigates dry-friction whip and whirl phenomena for a rigid rotor contacting at two bearing locations. The idea originated with a paper by Clark (2009, Investigation of the NRG #40 Anemometer Slowdown, American Wind Energy Association, Windpower 2009, Chicago, IL, pp. 1-16) on an anemometer undergoing dry-friction whip and whirl. The anemometer rotor was supported by two Teflon® bushings within an elastically supported housing. The dry-friction forces arose at the bushings. Prior models for dry friction whirl and whip have considered rub at one nonsupport location. The present analytical model consists of a rigid rotor connected to a rigid stator at two rubbing-contact locations. Analytical solutions are developed for the following normal reaction forces at the contact locations: (1) In phase, and (2) 180° out of phase. Analytical solutions are only possible for the same radius-to-clearance ratio (RCl) at the two rub locations and define regions where dry-friction whirl ...
Cells exposed to ionizing radiation die via different mechanisms, including apoptosis and mitotic catastrophe. To determine the frequency of mitotic catastrophe in tumor cells after irradiation, we used time-lapse imaging to track centrin-1 and histone H2B in U2OS osteosarcoma cells. We observed a d …
The Neurosurgical Atlas depends almost entirely on your donations. We are unable to continue the Atlas without a significant donation from you.. Please commit at least a yearly $250 donation to the Atlas.. Without this commitment, the Atlas will soon require a paid subscription and will become inaccessible to many surgeons around the world whose patients care depend on it. Please donate now!. ...
The ability of cancer cells to migrate through a complex three-dimensional (3D) environment is a hallmark event of cancer metastasis. Therefore, an in vitro migration assay to evaluate cancer cell migration in a 3D setting is valuable to examine cancer progression. Here, we describe such a simple migration assay in a 3D collagen-fibronectin gel for observing cell morphology and comparing the migration abilities of cancer cells. We describe below how to prepare the collagen-fibronectin gel castings, how to set up time-lapse recording, how to draw single-cell trajectories from movies and extract key parameters that characterize cell motility, such as cell speed, directionality, mean square displacement, and directional persistence. In our set-up, cells are sandwiched in a single plane between two collagen-fibronectin gels. This trick facilitates the analysis of cell tracks, which are for the most part 2D, at least in the beginning, but in a 3D environment. This protocol has been previously published in
where Vi is field voltage at time point i (ti), and Vi − 1 is field voltage at the previous time point (ti − 1). To correct for underlying baseline noise, the CBI for an equal duration of recording in the absence of bursts was subtracted from the burst CBI. This sensitive measure of burst intensity is useful for determining the effects of anti-epileptic interventions (Korn et al., 1987; Tallent and Siggins, 1999).. Whole-cell recordings were made from individual granule cells located within the dentate gyrus, identified under infrared differential interference contrast video microscopy with a Carl Zeiss Axioskop 2 FS plus microscope. Patch pipettes were pulled from borosilicate glass (WPI) and filled with the following (in mm): 120 Cs-gluconate, 10 HEPES, 0.1 EGTA, 15 CsCl2, 4 MgCl2, 4 Mg-ATP, and 0.3 Na2-GTP, pH 7.25 (adjusted with 1 m CsOH; 285-290 mOsm; patch electrode resistance, 3-6 MΩ). Electrophysiological data were obtained from granule cells that had a holding current between ...
The Molecular Expressions DIC image gallery contains a wide spectrum of stained and unstained specimens, including transparent living and fixed cells and tissues.
Description • The Zeiss-Biorad Confocal microscope unit features the following capabilities DICM (Differential Interference Contrast Micros...
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Most organisms that live in aquatic systems are too small to be seen with the naked eye. To help identify and quantify these organisms, several types of microscopes are utilized. These include flourescence, inverted plankton, dissecting, and video microscopy ...
Instagram has released its new app, Instagram Hyperlapse, a fun and easy way to film and create your own time lapse videos in high quality from your phone.
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Although time lapse photography and extended exposures are nothing new, It is the melding of these techniques in new ways that allow the of viewing nature in a whole new way ...
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This video shows an example of a viability measurement performed with the Cellometer K2. 20 microliters of PMBC sample is mixed with AO/PI solution and then loaded into the Cellometer counting chamber.
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Sluder, Greenfeld; Matsudaira, Paul T.; Wolf, David E. (1998-03-12). Video Microscopy. Gulf Professional Publishing. ISBN ... Video Microscopy (1998) "Paul Thomas Matsudaira" (PDF). National University of Singapore. October 2017. Retrieved 2017-10-16. " ... Before moving to NUS, he taught biology students at Singapore via online videos, and taught at study camps while on summer ...
ISBN 978-0-521-88169-2. Randy O. Wayne (16 December 2013). Light and Video Microscopy. Academic Press. ISBN 978-0-12-411536-1. ... computer monitors and video projectors, rendering the previous CRT technology essentially obsolete. The use of polarization in ... anisotropy Glan-Taylor prism Kerr effect Nicol prism Pockels effect Polarization rotator Polarized light microscopy Polarizer ...
Randy O. Wayne (28 July 2010). Light and Video Microscopy. Academic Press. p. 72. ISBN 978-0-08-092128-0. Retrieved 24 April ... He encountered the work of Antoni van Leeuwenhoek, another lens grinder, in the field of microscopy which interested his father ...
ISBN 9780128143711) Light and Video Microscopy, 2009, Elsevier/Academic Press. (ISBN 9780080921280) Light and Video Microscopy ... The third edition of Light and Video Microscopy is dedicated to Peter K. Hepler. In 2010, Wayne proposed a theory of light that ... He has a deep interest in teaching science and teaches Plant Cell Biology and Light and Video Microscopy. He has taught a ... "Light and Video Microscopy". YouTube. Albert R. Mann Library, Cornell University. Retrieved July 26, 2016. Botanist from Boston ...
Springer), p. 198 Wayne, Randy O. (2014). Light and Video Microscopy. (Academic Press, Elsevier), p. 43 Buchwald, Jed Z. (1989 ... "The discovery by Brian J Ford of Leeuwenhoek's original specimens, from the dawn of microscopy in the 16th century". Brianjford ... Named after Frits Zernike, the Dutch optical physicist, and the inventor of phase contrast microscopy, they play an important ...
Gilbert SF (2000). "Regeneration". Developmental Biology (6th ed.). "Olympus Microscopy Resource Center , Pond Life Video ...
He also was the first to develop video microscopy, and wrote a major textbook on the subject. Consistent with Inoué's ... Inoue S, Spring K (1997). Video Microscopy: the Fundamentals. Plenum Press, New York. Cameron, Lisa A. (2019). "Shinya Inoué: A ... His research field was the visualization of dynamic processes within living cells using light microscopy. Inoué can be ... Long Career Advancing Microscopy and Cell Biology (1921-2019)". The Biological Bulletin. 237 (3): 211-212. doi:10.1086/707517. ...
CS1 maint: discouraged parameter (link) Adam, B.; Mengel, M. (7 August 2015). "Transplant biopsy beyond light microscopy". BMC ... Solez, K. (5 October 2015). Bridge Between Transplantation and Regenerative Medicine (video). 2015 Banff Conference for ... and electron microscopy. The 2017 Banff Conference was held in Barcelona, Spain. The 2019 Banff Conference was held in ... and included consideration of molecular pathology and tissue engineering pathology as well as traditional light microscopy, ...
Digital video microscopy has revealed the existence of an equilibrium hexatic phase as well as a strongly first-order liquid-to ... "Video Microscopy of Monodisperse Colloidal Systems". Annual Review of Physical Chemistry. 47: 421-462. Bibcode:1996ARPC...47.. ... In addition, confocal laser scanning microscopy has been used to observe crystal growth near a glass surface. Electro-optic ...
83, p. 238 (1995); Video Microscopy of Monodisperse Colloidal Systems, Annu. Rev. Phys. Chem., Vol. 47, p. 421 (1996) Tanaka, T ... Micrometre-scale colloidal particles are large enough to be observed by optical techniques such as confocal microscopy. Many of ...
Since Light field microscopy captures full volume information in a single frame, it is possible to monitor neural activity in ... If the latter is combined with an array of video cameras, one can capture and display a time-varying light field. This ... Kanade, T., Saito, H., Vedula, S. (1998). "The 3D Room: Digitizing Time-Varying 3D Events by Synchronized Multiple Video ... Levoy, M., Ng, R., Adams, A., Footer, M., Horowitz, M. (2006). "Light Field Microscopy", ACM Transactions on Graphics (Proc. ...
doi:10.1016/S0001-8686(97)00312-6. Crocker, J. C.; Grier, D. G. (1996). "Methods of Digital Video Microscopy for Colloidal ... Nakroshis, P.; Amoroso, M.; Legere, J.; Smith, C. (2003). "Measuring Boltzmann's constant using video microscopy of Brownian ... pair correlation function was achieved by a scanning tunneling microscopy in the case of 2D molecular gases. It has been noted ... "Pair correlation function of a 2D molecular gas directly visualized by scanning tunneling microscopy". The Journal of Physical ...
Flow visualization and flow cytometry with holographic video microscopy. Optics Express, 17(15), 13071-13079. Fillmore, D., ... Strategies for three-dimensional particle tracking with holographic video microscopy. Optics Express, 18(13), 13563. doi: ...
History of Electron Microscopy 1990s. *^ de Jonge, N.; Ross, F.M. (2011). "Electron microscopy of specimens in liquid". ... The video starts at 25×, about 6 mm across the whole field of view, and zooms in to 12000×, about 12 μm across the whole field ... Danilatos, G.D. (1986). "Environmental scanning electron microscopy in colour". Journal of Microscopy. 142: 317-325. doi: ... "Microscopy Today. 26 (3): 12-17. doi:10.1017/S1551929518000482.. *^ Hortolà, P. (2010). "Using digital color to increase the ...
Nintendo Video. Also in July, Passmorelab, in conjunction with The Collective, converted Memphis High's latest music video, ... Passmore then expanded its work to include stereoscopic microscopy. The company began converting Ripley's film archives into 3D ... as well as completing 3D music videos for Linkin Park's Waiting for the End, and for Hollywood Records' Plain White T's Rhythm ... "Slash In 3D! Guitarist Teams Up With Passmorelab To Convert Latest Video 'By The Sword' To 3D". September 1, ...
In: The Quest for the Invisible: Microscopy in the Enlightenment. Aldershot: Ashgate. Types of Protozoans and video Pond Life ...
Video Microscopy of Monodisperse Colloidal Systems, Annu. Rev. Phys. Chem., Vol. 47, p. 421 (1996). Tanaka, 1992, Phase ... Micrometre-scale colloidal particles are large enough to be observed by optical techniques such as confocal microscopy. Many of ...
Janicke B. "Digital holographic microscopy video showing cell division of unlabeled JIMT-1 breast cancer cells". The Cell - an ... Quantitative phase contrast microscopy has an advantage over fluorescent and phase contrast microscopy in that it is both non- ... Keller HE (2006), "Objective Lenses for Confocal Microscopy", Handbook of Biological Confocal Microscopy, Springer US, pp. 145- ... Magidson V, Khodjakov A (2013). "Circumventing photodamage in live-cell microscopy". Digital Microscopy. Methods in Cell ...
"Measuring Boltzmann's constant through holographic video microscopy of a single colloidal sphere". American Journal of Physics ...
Other programs such as VideoStitch make it possible to stitch videos, and Vahana VR enables real-time video stitching. Image ... It can be also used for manual stitching of whole microscopy samples. ActionShot panoramic photography Anaglyph 3D AutoStitch ... Breszcz, M.; Breckon, T. P. (August 2015). "Real-time Construction and Visualization of Drift-Free Video Mosaics from ... constructing high-quality stills from video" (PDF). Proceedings of the IEEE First International Conference on Image Processing ...
YouTube video. HyBiz TV. 8 May 2014. "Dr Santosh G Honavar Director CFS Group". YouTube video. HyBiz TV. 12 May 2014.. ... comparative evaluation of direct microscopy and culture results". Ann Ophthalmol. 25 (2): 68-71. PMID 8447653.CS1 maint: ...
YouTube video). Arakawa, Kazuharu (8 February 2018). Macrobiotus shonaicus - ショウナイチョウメイムシ - 3 (YouTube video). Arakawa, ... identified in Japan: Researchers characterize new species using microscopy, genetic analysis". ScienceDaily. Arakawa, Kazuharu ... 8 February 2018). Macrobiotus shonaicus - ショウナイチョウメイムシ - 1 (YouTube video). Arakawa, Kazuharu (8 February 2018). Macrobiotus ... Kazuharu (8 February 2018). Macrobiotus shonaicus - ショウナイチョウメイムシ - 4 (YouTube video).. ...
Fourier slice photography Light Field Microscopy video by Stanford Computer Graphics Laboratory. IEEE Spectrum article May 2012 ... Levoy, M; Zhang, Z; McDowall, I (2009). "Recording and controlling the 4D light field in a microscope". Journal of Microscopy. ... Levoy, M; Ng, R; Adams, A; Footer, M; Horowitz, M (2006). "Light Field Microscopy". ACM Transactions on Graphics. 25 (3): 924- ... A later version of the prototype added a light field illumination system consisting of a video projector (allowing ...
Quantitative settlement studies and video microscopy. Journal of Phycology, 33(6), 938-947. doi:10.1111/j.0022-3646.1997.00938. ...
Missing or empty ,title= (help)CS1 maint: discouraged parameter (link) "YouTube video: Building a small telescope". Iyer, V; ... third harmonic generation microscopy, fibre optics setups, biomedical instruments, eye surgery apparatus, telescopes, stellar ... "Compensation of spatial and temporal dispersion for acousto-optic multiphoton laser-scanning microscopy". Journal of Biomedical ...
Either an analog video camera or a digital video camera can be used for robotic microscopy. Another form of real-time ... microscopy involves utilizing a high resolution video camera mounted on a path lab microscope to send live digital video of a ... Real-time robotic microscopy systems and virtual slides allow a consultant pathologist the opportunity to evaluate ... An echo-cancelling microphone at each end of the video conference allows the pathologist to communicate with the person moving ...
This reaction can be followed by video-scanning tunneling microscopy with sub-molecular resolution. In the Meisenheimer ...
Huang uses atom-by-atom electron microscopy to characterise these interfacial atoms. She combines microscopy with transient ... Huang created videos that make it possible to understand the liquid state of glass. Defects and dopants can have significant ... In particular, Huang works on aberration-corrected electron microscopy to study two-dimensional materials. To visualise glass ... She develops transmission electron microscopy to investigate two-dimensional materials. During her PhD she discovered the ...
Cutting-edge development of confocal laser scanning microscopy now allows better than standard video rate (60 frames per second ... The Development of a Modern Microscopy". Imaging & Microscopy.. online. *^ a b c Barry R. Masters: Confocal Microscopy And ... Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM), is an optical imaging technique for increasing ... Barry R. Masters: Confocal Microscopy And Multiphoton Excitation Microscopy. The Genesis of Live Cell Imaging. SPIE Press, ...
"Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies". ... fixing either the microtubule or motor proteins to a microscope slide then visualizing the slide with video-enhanced microscopy ...
Videos: Ebola outbreak response - World Health Organization. *. "Ebola Preparedness and Response". U.S. Food and Drug ... Ryabchikova, Elena I.; Price, Barbara B. (2004). Ebola and Marburg Viruses: A View of Infection Using Electron Microscopy. ... "Ebola: The Truth Behind The Outbreak (Video) l". Mabalo Lokela Archives - Political Mol. Archived from the original on 12 July ... "Modern uses of electron microscopy for detection of viruses". Clinical Microbiology Reviews (Review). 22 (4): 552-63. doi ...
en) Kategori:Stevens Institute of Technology - bilder, video eller lyd på Wikimedia Commons. ... Electron Microscopy Laboratory. *Center for Mass Spectrometry. Stevens har samarbeid med Kongsbergindustrien og de norske ...
Video on X-ray inspection and industrial computed tomography, Karlsruhe University of Applied Sciences ... image intensifiers are analog devices that readily convert the acquired X-ray image into one visible on a video screen. This ...
After the spores were analyzed by microscopy, it was determined that the cells were very similar to Bacillus sphaericus which ... Malachite green - endospore staining technique (video). *Resistance of Bacillus Endospores to Extreme Terrestrial and ... Visualising endospores under light microscopy can be difficult due to the impermeability of the endospore wall to dyes and ...
... ranging from 2D crystallization of proteins to Brewster angle microscopy. The LB trough's general objective is to study the ... Langmuir Trough Video (Youtube). Retrieved from " ...
Scanning electron microscopy[edit]. Fly larvae and fly eggs are used to aid in the determination of a PMI. In order for the ... When scanning electron microscopy is not available, a faster, lower cost technique is potassium permanganate staining. The ... A study in 2007 demonstrates a technique that can use scanning electron microscopy (SEM) to identify key morphological features ... "Identification of fly eggs using scanning electron microscopy for forensic investigations". Micron. 39 (7): 802-7. doi:10.1016/ ...
"Microscopy Research and Technique. 61 (2): 203-23. doi:10.1002/jemt.10330. PMID 12740827.. ... Depreter M, Espeel M, Roels F (Jun 2003). "Human peroxisomal disorders". Microscopy Research and Technique. 61 (2): 203-23. doi ...
Multiple white cells seen in the urine of a person with a urinary tract infection using a microscopy. ... urine microscopy, looks for the presence of red blood cells, white blood cells, or bacteria. Urine culture is deemed positive ... shown between white blood cells in urinary microscopy. These changes are indicative of a urinary tract infection. ...
Microscopy. *Scanning electron microscope (SEM). *Transmission electron microscope (TEM). Thermochemistry. *Calorimeter * ...
Efforts are made to ensure laboratory safety videos are both relevant and engaging.[14] ... Microscopy. *Scanning electron microscope (SEM). *Transmission electron microscope (TEM). Thermochemistry. *Calorimeter * ... "Creating Customized, Relevant, and Engaging Laboratory Safety Videos". Journal of Chemical Education. 84 (10): 1727. Bibcode ...
... drawback of magnetic tweezers is the low temporal and spatial resolution due to the data acquisition via video-microscopy.[3] ... In the case of atomic force microscopy, it may also be hard to discriminate the interaction of the tip with the studied ... with magnets that are used to manipulate the magnetic particles whose position is measured with the help of video microscopy. ... optical tweezers and atomic force microscopy. The magnetic interaction is highly specific to the used superparamagnetic ...
Slayter, Elizabeth M.; Slayter, Henry S. (1992). Light and Electron Microscopy. Cambridge University Press. ISBN 978-0-521- ... Videos. *16-year-long study tracks stars orbiting Milky Way black hole ...
J Cardiovasc Surg (Turin) 2000 Apr;41(2):193-202 (video) *^ a b c Schoenwolf, Gary C.; et al. (2009). "Development of the ... Bertazzo, S. et al. Nano-analytical electron microscopy reveals fundamental insights into human cardiovascular tissue ...
These make it especially useful for wide-angle camera lenses, microscopy, and the core part of optical fibers.[62][63] It has ... Germanium at The Periodic Table of Videos (University of Nottingham). Retrieved from " ...
New England Journal of Medicine procedure videos: Male Urethral catheterization. *Ohio State University Patient Education ...
"In: The Quest for the Invisible: Microscopy in the Enlightenment. Aldershot: Ashgate. ... Plankton Chronicles - Protists - Cells in the Sea - video. *Holt, Jack R. and Carlos A. Iudica. (2013). Diversity of Life. http ...
Commons: Giardia lamblia - Album me fotografi dhe/apo video dhe materiale multimediale ... cyst as imaged at different instrument settings by confocal microscopy.Bar = 10 micrometers.. (A) is the cyst imaged by ...
In the United States, forensic pathologists typically complete at least one year of additional training (a fellowship) after completing an anatomical pathology residency and having passed the "board" examination administered by The American Board of Pathology or The American Osteopathic Board of Pathology ("board-certified"). Becoming an anatomic pathologist in the United States requires completing a residency in anatomic pathology, which is on-the-job training one must perform upon completing medical school before one may practice unsupervised. Anatomic pathology (as it is called) by itself is a three-year residency. Most U.S. pathologists complete a combined residency in both anatomic and clinical pathology, which requires a total of four years. In the United States, all told, the education after high school is typically 13-15 years in duration (4 years undergraduate training + 4 years medical school + 4-5 years residency [anatomic and clinical pathology combined] + 1-2 year forensic pathology ...
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  • Given fluorescence microscopy images, existing methods extract neurofilament movement patterns by manual tracking. (
  • Rotating fluorescence light microscopy footage of a cross-section through papillae in tissue from a tongue. (
  • Microscopical studies in biology have relied on two complementary microscope technologies - light (fluorescence) microscopy and electron microscopy. (
  • Visualizing intracellular events in vivo by combined video fluorescence and 3-D electron microscopy. (
  • The combination of the capability of in vivo fluorescence video microscopy with the power of resolution of electron microscopy (EM) has been described. (
  • SIM is faster than other forms of super-resolution fluorescence microscopy. (
  • Betzig was one of three scientists awarded the 2014 Nobel Prize in Chemistry for the development of another innovative microscopic imaging technology, known as super-resolved fluorescence microscopy. (
  • The high level of light required by super-resolved fluorescence microscopy damages the cells and causes the markers that scientists use to track molecules to fade quickly. (
  • This rich parameter space is not accessible by fluorescence microscopy, but it is within reach of interferometric scattering (iSCAT) particle tracking. (
  • Fluorescence microscopy in three dimensions. (
  • Fluorescence Microscopy of Living Ceils in Culture: B. Quantitative Fluorescence Microscopy-Imaging and Spectroscopy. (
  • Fluorescence digital imaging microscopy in cell biology. (
  • Available in 4X-100X sizes, Olympus Plan Achromatic Objectives provide precise field flatness up to F.N.22 in brightfield, darkfield, or fluorescence microscopy. (
  • Fluorescence Microscopy. (
  • A comprehensive introduction to advanced fluorescence microscopy methods and their applications. (
  • A team of researchers has developed a microscope that is capable of live imaging at double the resolution of fluorescence microscopy using structured illumination. (
  • Scanning probe microscopy and electron microscopy both have superior spatial resolution, but typically are not compatible with three-dimensional micromanipulation techniques, particularly under environmental conditions. (
  • In vivo dynamics and ultrastructure of individual ER-to-Golgi carriers studied using correlative video-light-electron microscopy. (
  • New York, NY -- ( SBWIRE ) -- 08/13/2019 -- A recent market intelligence study on the Video Electron Microscopy market incorporates proprietary techniques and assessment tools to screen the Video Electron Microscopy market for the forecast period, 2019-2026. (
  • Additionally, valuable insights pertaining to the market size, share and growth rate of Video Electron Microscopy market offers a greater chance of success for all - business owners, products, and innovative technology. (
  • Incorporated with Info-graphics, charts, 35 tables and 55 figures, this 117-page research report "Video Electron Microscopy Market Size, Type Analysis, Application Analysis, End-Use Industry Analysis, Regional Outlook, Competitive Strategies And Forecasts, 2019 - 2026" is based on a complete research of the entire Global market and covering all its sub-segments through comprehensively thorough classifications. (
  • The pro-active approach towards analysis of investment feasibility, significant return on investment, supply chain management, import and export status, consumption volume and end-use offers more value to the overall statistics on the Video Electron Microscopy market. (
  • The size of the Video Electron Microscopy market is viewed in terms of the Share of Market, Total Available Market as well as Served Available Market. (
  • Analysis of percentage or the size of the Total Available Market based on the type of product, technology, regional constraints and others form an important part of the Video Electron Microscopy report. (
  • Focuses on the key Video Electron Microscopy manufacturers, to study the capacity, production, value, market share and development plans in future. (
  • Technician works inside 'Electron Microscopy' room at National Aeronautics and Space Administration (NASA) in United States. (
  • Electron Microscopy' written outside the door of a room. (
  • Scanning Probe Microscopy: Feeling out Electron Pairs 41:23 - Chapter 4. (
  • when combined with super-resolution microscopy, it unveiled details of ultrastructural organization, such as centriolar chirality, that could otherwise be observed only by electron microscopy. (
  • Kinesin from pig brain studied by electron microscopy. (
  • Researchers at Lawrence Berkeley National Laboratory and UC Berkeley have combined cutting-edge cryo-electron microscopy (cryo-EM) with computational molecular modeling to produce a near atomic-resolution model of the interaction between microtubules - crucial components of eukaryotic cell ultrastructure - and microtubule-associated proteins called tau. (
  • Cryo electron microscopy is booming, with new atomic structures appearing every week and new facilities being installed at research centers across the globe. (
  • Cryo-electron microscopy (cryo-EM)-which enables the visualization of viruses, proteins, and other biological structures at the molecular level-is a critical tool used to advance biochemical knowledge. (
  • Complete capsid of bacteriophage P22 generated with validated atomic models that were derived from a high-resolution cryo-electron microscopy density map. (
  • This is a great example of how to exploit electron microscopy technology and combine it with new computational methods to determine a bacteriophage's structure," said Paul Adams, Berkeley Lab's Molecular Biophysics & Integrated Bioimaging division director and a co-author of the paper. (
  • Biochemists at the University of Zurich have used cryo-electron microscopy to determine the detailed architecture of the chloride channel TMEM16A. (
  • There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy, along with the emerging field of X-ray microscopy. (
  • Optical microscopy and electron microscopy involve the diffraction, reflection, or refraction of electromagnetic radiation/electron beams interacting with the specimen, and the collection of the scattered radiation or another signal in order to create an image. (
  • This process may be carried out by wide-field irradiation of the sample (for example standard light microscopy and transmission electron microscopy) or by scanning a fine beam over the sample (for example confocal laser scanning microscopy and scanning electron microscopy). (
  • Electron microscopy showed that these buttons were packed with budding viral crescents. (
  • Many people will never have heard of cryo-electron microscopy before the announcement that Jacques Dubochet, Joachim Frank and Richard Henderson had won the 2017 Nobel Prize in chemistry for their work developing this technology. (
  • Cryo-electron microscopy - or cryo-EM - is an imaging technology that allows scientists to obtain pictures of the biological "machines" that work inside our cells. (
  • This thoroughly revised and updated Fourth Edition of a time-honored text provides the reader with a comprehensive introduction to the field of scanning electron microscopy (SEM), energy dispersive X-ray spectrometry (EDS) for elemental microanalysis, electron backscatter diffraction analysis (EBSD) for micro-crystallography, and focused ion beams. (
  • Holography was invented by Dennis Gabor to improve electron microscopy. (
  • One of today's sharpest imaging tools, super-resolution microscopy, produces sparkling images of what until now has been the blurry interior of cells, detailing not only the cell's internal organs and skeleton, but also providing insights into cells' amazing flexibility. (
  • Super-Resolution Microscopy. (
  • Confocal and deconvolution microscopies overcome this limitation by scanning through the sample and assembling the resulting axial slices into a volumetric data set. (
  • Howon Seo, Yoonha Hwang, Kibaek Choe, and Pilhan Kim, "In vivo quantitation of injected circulating tumor cells from great saphenous vein based on video-rate confocal microscopy," Biomed. (
  • In this study, we implemented a custom-design video-rate confocal microscopy system in capable of direct visualization of fast flowing CTC at great saphenous vein (GSV) of a live animal model in vivo . (
  • Correlative Confocal Raman Microscopy for 2D Materials. (
  • What is Fluorescent Confocal Microscopy? (
  • Confocal and multiphoton microscopy are non-invasive fluorescent imaging techniques that use lasers of different wavelengths to excite the sample and produce the image. (
  • Dage-MTI's RealView HD microscope cameras combine Toshiba's imaging technology with specially formatted preset values for use in critical microscopy applications. (
  • Is the Foldscope microscope suitable for Amateur Microscopy and Citizen Science? (
  • As reported in Science , the Howard Hughes Medical Institute team used a combination of adaptive optics and lattice-sheet microscopy to create a 10-foot-long microscope for creating movies of living organisms. (
  • Ovizio Imaging System, an innovative quantitative microscopy company specializing in life science solutions, announces today the launch of its new product, the iLine F, an in-line suspension cell-monitoring microscope. (
  • in visible range, the resolution of optical microscopy is limited to approximately 0.2 micrometres (see: microscope) and the practical magnification limit to ~1500x. (
  • The completely integrated, open-access design and exclusive Microscope Image Registration and Overlay capability fully leverages the power of both light microscopy and AFM. (
  • A wet mount of Naegleria fowleri trophozoites cultured from the CSF of a patient with primary amebic meningoencephalitis (PAM) viewed using phase contrast microscopy. (
  • Digital holographic microscopy thus makes it possible to visualize and quantify transparent objects and is therefore also referred to as quantitative phase-contrast microscopy. (
  • This image is similar to that obtained by phase contrast microscopy but without the bright diffraction halo. (
  • The physicist Stefan Hell's discoveries were a revolution in the field of microscopy. (
  • The field of microscopy (optical microscopy) dates back to at least the 17th-century. (
  • We report the development of functional photoacoustic microscopy capable of video-rate high-resolution in vivo imaging in deep tissue. (
  • In vivo functional human imaging using photoacoustic microscopy response. (
  • IRVINE, Calif., April 4, 2011 - Toshiba Imaging Systems Division has announced an OEM relationship with Dage-MTI to introduce two high definition (HD) cameras designed for microscopy applications. (
  • 2010 - CAMBRIDGE, Mass. - A novel type of biomedical imaging, made possible by new advances in microscopy from scientists at Harvard University, is so fast and sensitive it can capture "video" of blood cells squeezing through capillaries. (
  • Recent developments have allowed the acquisition of SRS images in reflection mode and at video-rate imaging speed in living mice and humans. (
  • We were able to speed the collection of data by more than three orders of magnitude, attaining video-rate imaging. (
  • In this short video, Olympus introduces its cellVivo incubation system for precise and ergonomic environmental control of advanced live cell imaging. (
  • Membrane imaging by simultaneous second-harmonic generation and two-photon microscopy: errata. (
  • However, within the last few years the combination of novel optical microscopic techniques with advanced video and digital image processing technology has resulted in dramatic improvements in the quality of light microscopic images, leading to a true renaissance in the use of light microscopy for the imaging of both fixed and living biological specimens. (
  • The recent introduction of a practical video-rate AFM has improved imaging speeds by yet another order of magnitude and now makes it possible to capture movies with a temporal resolution better than a second with simple operation. (
  • Please join us for this free workshop that will give an introduction to video-rate imaging and biophysical applications with lectures and hands-on demonstrations with Asylum Research Cypher™ VRS Video-Rate AFM. (
  • ZEN - ZEISS Efficient Navigation is the single user interface you will see on all light microscopy imaging systems from ZEISS. (
  • Available in 20X and 50X magnifications, Mitutoyo Glass Thickness-Compensated Infinity Corrected Objectives are suited for imaging objects through glass up to 3.5 mm thick in brightfield inspection microscopy and life sciences applications. (
  • X-ray microscopy is three-dimensional and non-destructive, allowing for repeated imaging of the same sample for in situ or 4D studies, and providing the ability to "see inside" the sample being studied before sacrificing it to higher resolution techniques. (
  • Both the scattered and unscattered laser light are collected by an oil-immersion objective lens and relayed to a video camera, which records their interference as a hologram. (
  • replaces the incandescent illuminator of conventional bright-field microscopy with a collimated coherent light source. (
  • Now high-resolution video microscopy is shedding light on how it can carefully use this adhesive, like a super-precise application of superglue, to stick on surfaces in wet environments. (
  • Video-enhanced light microscopy of a terminally differentiated mouse megakaryocyte forming proplatelets in vitro. (
  • Historically, light microscopy has been limited in its ability to resolve closely spaced objects, with the best microscopes only able to resolve objects separated by 200 nm or more. (
  • iBiology has produced over 80 videos by the world's leading scientists including seminars about research in fields from ecology to biophysics, a 65-video course on light microscopy, and over 90 short talks on topics such as professional development, careers, science and society, and education. (
  • Xie's team has already used SRS microscopy to track migration of medications in skin, shedding new light on the absorption of topical drugs. (
  • Using this approach, the scientists were able to collect and analyze almost 30 percent of the laser light directed at a biological sample, a more than 30-fold increase over previous SRS microscopy. (
  • For a long while, to apply microscopy with focused light meant that details smaller than half the wavelength of light (200 nm) could not be resolved. (
  • 164170670253 Boeckeler VIA-100 Video Image Measurement System w/ Controller & Y/C Microscopy Model: VIA-100KModel: VIA-Y/CModel: KS-30 Items are in good condition with minor cosmetic wear.Green indicator light powers on when VIA-100 switch is turned on.I have no further way of testing. (
  • If you're not watching recent work in biology, you might have thought that light microscopy hit its limits years ago. (
  • Coherent scattering in multi-harmonic light microscopy. (
  • Weisenburger, S. & Sandoghdar, V. Light microscopy: an ongoing contemporary revolution. (
  • By using a combination of semiquantitative light microscopy, video microscopy, and a biochemical assay, we show that this pathway is used by the endogenous ligand transferrin (Tf) and its receptor. (
  • 2003) Health Survey for England 2002: download Light and video microscopy 2: unable and instrumental research, London: The Stationery Office. (
  • Benedict and Mead existed found for back submitting stunning download Light and video within a video and offering the server as a genetic web. (
  • The Zeiss-Nomarski differential interference equipment for transmitted light microscopy. (
  • This type of microscopy is necessary when items or features are too small to be imaged by light. (
  • Specifically, we acquired 4D (3D + time) images with light sheets by selective plane illumination microscopy (SPIM) for rapid scanning and deep axial penetration during zebrafish morphogenesis. (
  • Short targetted videos that will boil down the principles of light microscopy for biologist and help you understand what you are doing. (
  • T he Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA recently named the winners of its 2019 UCLA-wide light microscopy image and video contest. (
  • Optical or light microscopy involves passing visible light transmitted through or reflected from the sample through a single lens or multiple lenses to allow a magnified view of the sample. (
  • This is the first title on the topic designed specifically to allow students and researchers with little background in physics to understand both microscopy basics and novel light microscopy techniques. (
  • Actually, DHM has mostly been applied to light microscopy. (
  • The lateral optical resolution of digital holographic microscopy is equivalent to the resolution of traditional light microscopy. (
  • DHM is diffraction-limited by the numerical aperture, in the same way as traditional light microscopy. (
  • Other closely related microscopy methods to digital holographic microscopy are interferometric microscopy, optical coherence tomography and diffraction phase microscopy. (
  • A high speed video microscopy system with an automated algorithm to detect pacemaker location and calculate the propagation velocity, speed, duration, and frequency of the contractions is presented in this paper. (
  • Using stimulated emission depletion microscopy and other techniques, Stefan Hell explores new ways to examine living cells noninvasively. (
  • In addition, the software offers two options for time-lapse microscopy. (
  • Contrary to prior observations and assumptions, time-lapse microscopy reveals proplatelet processes to be extremely dynamic structures that interconvert reversibly between spread and tubular forms.Growth and extension of proplatelet processes is associated with repeated bending and bifurcation, which results in considerable amplification of free ends.These aspects are inhibited by cytochalasin B and, therefore, are dependent on actin. (
  • Contrary to prior observations and assumptions, time-lapse microscopy reveals proplatelet processes to be extremely dynamic structures that interconvert reversibly between spread and tubular forms. (
  • Bright-field microscopy is comparable to looking through a glass window: one sees not the glass but merely the dirt on the glass. (
  • SCI welcomed Prof Robin Clark to present a Public evening lecture on Raman Microscopy and the Identification of Forgery in Artwork on 19 November 2014. (
  • Conventional infrared (IR) microscopy has low spatial resolution and requires desiccated samples, while spontaneous Raman microscopy requires high laser power and long integration times, limiting use in live specimens. (
  • Esben Ravn Andresen, Géraud Bouwmans, Serge Monneret, and Hervé Rigneault, "Toward endoscopes with no distal optics: video-rate scanning microscopy through a fiber bundle," Opt. (
  • We achieve video-rate images by galvanometric scanning of the phase tilt at the proximal end. (
  • SRS microscopy provides equivalent insights through real-time scanning. (
  • Scanning Probe Microscopy is a technique used to produce images of surfaces using mechanical probes. (
  • Types of SPM include atomic force microscopy (AFM), scanning tunneling microscopy (STM) and near field scanning optical microscopy (NSOM). (
  • Lec 4 - Coping with Smallness and Scanning Probe Microscopy" Freshman Organic Chemistry (CHEM 125) This lecture asks whether it is possible to confirm the reality of bonds by seeing or feeling them. (
  • In the last 25 years various realizations of Scanning Probe Microscopy have enabled chemists to "feel" individual molecules and atoms, but not bonds. (
  • This creates a problem in the field of scanning acoustic microscopy since high speed. (
  • This gallery contains digital videos captured with the FV300 and FV1000 under a variety of experimental conditions and scanning modes. (
  • Scanning probe microscopy involves the interaction of a scanning probe with the surface of the object of interest. (
  • Schitter, G. / Mechatronics and control for scanning probe microscopy at video-rate . (
  • Differential interference contrast (DIC) microscopy, also known as Nomarski interference contrast (NIC) or Nomarski microscopy, is an optical microscopy technique used to enhance the contrast in unstained, transparent samples. (
  • Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). (
  • Once you login, Internet is not required to watch videos. (
  • In this video by Science , scientists adapt firefly biology to create bioluminescent cells detectable from outside the body. (
  • The development of microscopy revolutionized biology, gave rise to the field of histology and so remains an essential technique in the life and physical sciences. (
  • Quantitative, high-speed three-dimensional (3D) video microscopy revealed the rapid formation of micrometer-sized "buttons" containing oligomerized viral Gag protein. (
  • Here we describe ultrastructure expansion microscopy (U-ExM), an extension of expansion microscopy that allows the visualization of preserved ultrastructures by optical microscopy. (
  • Schematic representation of holographic video microscopy. (
  • Each holographic image in the video stream is a time-resolved snapshot of the three-dimensional distribution of scatterers in the microscope's field of view. (
  • We use holographic video microscopy to track the three-dimensional translational and rotational diffusion of copper oxide nanorods suspended in water. (
  • Digital holographic microscopy (DHM) is digital holography applied to microscopy. (
  • Digital holographic microscopy distinguishes itself from other microscopy methods by not recording the projected image of the object. (
  • The phase shift image is unique for digital holographic microscopy and gives quantifiable information about optical distance. (
  • Lidai Wang , Konstantin I. Maslov , Wenxin Xing, Alejandro Garcia-Uribe , Lihong V. Wang , "Video-rate functional photoacoustic microscopy at depths," Journal of Biomedical Optics 17(10), 106007 (1 October 2012). (
  • This remarkable live-action view was produced using one of two new forms of extended-resolution, structured illumination microscopy (SIM). (
  • Although the software control system provides efficient and reproducible microscopy and microanalysis, the user must understand the parameter space wherein choices are made to achieve effective and meaningful microscopy, microanalysis, and micro-crystallography. (
  • iBiology ( is a collection of open-access online videos by scientists about research and discoveries that cover a wide variety of life science subjects. (
  • Scientists currently use a variety of techniques to view biomolecules, but most have significant limitations that are sidestepped by SRS microscopy. (
  • The images and videos were chosen by a panel of scientists, microscopists and science communicators for their exceptional structure, color, composition, informational content, innovation, technical difficulty and visual impact. (
  • In this video by Science, scientists have manipulated spirulina, a microscopic plant and food supplement, to travel through people in response to magnetic signals. (
  • Fundraiser: Help Build a Free Image/Video Library of the Microscopic World! (
  • For the first time, SRS microscopy makes possible label-free chemical movies, with streaming footage at the subcellular level, catching video of proteins, lipids, and water within cells. (
  • Because SRS microscopy works by detecting the intrinsic vibrations in chemical bonds between atoms, it doesn't require intrusive fluorescent labeling. (
  • The software enables the capture of slow-motion videos. (
  • In contrast to single time point snapshots, live-cell video microscopy enables a wide variety of host cells and pathogens to be studied as continuous sequences over lengthy time periods, providing spatial and temporal information on a broad range of dynamic processes, including cell migration, replication and vesicular trafficking. (
  • The time interval for both images and video can be set to user needs as well as automated start and stop of the recording. (
  • Continuous acquisition of video-rate images at GSV revealed the highly dynamic time-dependent changes in the number of intravenously injected circulating tumor cells. (
  • Microscopy in which television cameras are used to brighten magnified images that are otherwise too dark to be seen with the naked eye. (
  • Alternatively, store your images as OME-TIFF, the image format specification of the Open Microscopy Environment, to facilitate cross-plattform image data exchange even including your valuable metadata. (
  • EB3 often finds use in microscopy applications as a marker of microtubules. (
  • A chimera of EB3 and mApple was employed to visualize the microtubules present in the African green monkey kidney cells (CV-1 line) shown in this digital videos sequence. (
  • Fingerprint Dive into the research topics of 'Live cell video microscopy of phagocytosis of fungal pathogens. (
  • This video from SciShow breaks down the science and some of the latest research into cancer-vaccine development into simple scientific terms. (
  • We have resolved the ultrastructure of the megakaryocyte cytoskeleton at specific stages of proplatelet morphogenesis and correlated these structures with cytoplasmic remodeling events defined by video microscopy. (
  • These Piezo nanopositioning stages & positioners are essential tools for high-resolution metrology and microscopy applications due to their sub-atomic resolution and extremely fast response times. (
  • Watch this video for more information on these stages and positioners and how they benefit microscopy, neuroscience, and metrology applications. (
  • Coherent anti-Stokes Raman scattering (CARS) microscopy, pioneered by Xie's own group, lacks the contrast to image most molecules beyond lipids. (
  • New observations on cell architecture and dynamics by video-enhanced contrast optical microscopy. (
  • A huge selection of microscopy techniques are available to increase contrast or label a sample. (
  • It took MRI more than 30 years to reach patients, but we're already looking forward with great anticipation to applications of SRS microscopy in hospitals. (
  • This video provides background on the technology and discusses initial clinical applications. (
  • I have put together for you some microscopy products in an Amazon Influencer Shop, so that you do not have to look for them! (
  • microscopy and newer far-field optical approaches can provide resolutions better than 20 nm, and in principle are able to resolve molecular detail. (
  • Optical sectioning microscopy: cellular architecture in three dimensions. (