Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
Stable elementary particles having the smallest known negative charge, present in all elements; also called negatrons. Positively charged electrons are called positrons. The numbers, energies and arrangement of electrons around atomic nuclei determine the chemical identities of elements. Beams of electrons are called CATHODE RAYS.
The process by which ELECTRONS are transported from a reduced substrate to molecular OXYGEN. (From Bennington, Saunders Dictionary and Encyclopedia of Laboratory Medicine and Technology, 1984, p270)
Electron microscopy in which the ELECTRONS or their reaction products that pass down through the specimen are imaged below the plane of the specimen.
A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
A type of scanning probe microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. The vertical position is recorded as a spring attached to the probe rises and falls in response to peaks and valleys on the surface. These deflections produce a topographic map of the sample.
Microscopy in which the samples are first stained immunocytochemically and then examined using an electron microscope. Immunoelectron microscopy is used extensively in diagnostic virology as part of very sensitive immunoassays.
A type of TRANSMISSION ELECTRON MICROSCOPY in which the object is examined directly by an extremely narrow electron beam scanning the specimen point-by-point and using the reactions of the electrons that are transmitted through the specimen to create the image. It should not be confused with SCANNING ELECTRON MICROSCOPY.
Electron microscopy involving rapid freezing of the samples. The imaging of frozen-hydrated molecules and organelles permits the best possible resolution closest to the living state, free of chemical fixatives or stains.
A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.
Fluorescence microscopy utilizing multiple low-energy photons to produce the excitation event of the fluorophore. Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal confocal microscopes. Ultimately this allows spatial isolation of the excitation event, enabling deeper imaging into optically thick tissue, while restricting photobleaching and phototoxicity to the area being imaged.
A type of scanning probe microscopy in which a very sharp conducting needle is swept just a few angstroms above the surface of a sample. The tiny tunneling current that flows between the sample and the needle tip is measured, and from this are produced three-dimensional topographs. Due to the poor electron conductivity of most biological samples, thin metal coatings are deposited on the sample.
Microscopy in which television cameras are used to brighten magnified images that are otherwise too dark to be seen with the naked eye. It is used frequently in TELEPATHOLOGY.
A form of interference microscopy in which variations of the refracting index in the object are converted into variations of intensity in the image. This is achieved by the action of a phase plate.
Microscopy using polarized light in which phenomena due to the preferential orientation of optical properties with respect to the vibration plane of the polarized light are made visible and correlated parameters are made measurable.
The science and application of a double-beam transmission interference microscope in which the illuminating light beam is split into two paths. One beam passes through the specimen while the other beam reflects off a reference mirror before joining and interfering with the other. The observed optical path difference between the two beams can be measured and used to discriminate minute differences in thickness and refraction of non-stained transparent specimens, such as living cells in culture.
Identification and measurement of ELEMENTS and their location based on the fact that X-RAYS emitted by an element excited by an electron beam have a wavelength characteristic of that element and an intensity related to its concentration. It is performed with an electron microscope fitted with an x-ray spectrometer, in scanning or transmission mode.
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.
Scanning microscopy in which a very sharp probe is employed in close proximity to a surface, exploiting a particular surface-related property. When this property is local topography, the method is atomic force microscopy (MICROSCOPY, ATOMIC FORCE), and when it is local conductivity, the method is scanning tunneling microscopy (MICROSCOPY, SCANNING TUNNELING).
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Preparation for electron microscopy of minute replicas of exposed surfaces of the cell which have been ruptured in the frozen state. The specimen is frozen, then cleaved under high vacuum at the same temperature. The exposed surface is shadowed with carbon and platinum and coated with carbon to obtain a carbon replica.
A multisubunit enzyme complex containing CYTOCHROME A GROUP; CYTOCHROME A3; two copper atoms; and 13 different protein subunits. It is the terminal oxidase complex of the RESPIRATORY CHAIN and collects electrons that are transferred from the reduced CYTOCHROME C GROUP and donates them to molecular OXYGEN, which is then reduced to water. The redox reaction is simultaneously coupled to the transport of PROTONS across the inner mitochondrial membrane.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
The rate dynamics in chemical or physical systems.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
A multisubunit enzyme complex that contains CYTOCHROME B GROUP; CYTOCHROME C1; and iron-sulfur centers. It catalyzes the oxidation of ubiquinol to UBIQUINONE, and transfers the electrons to CYTOCHROME C. In MITOCHONDRIA the redox reaction is coupled to the transport of PROTONS across the inner mitochondrial membrane.
Characteristics or attributes of the outer boundaries of objects, including molecules.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Elements of limited time intervals, contributing to particular results or situations.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Established cell cultures that have the potential to propagate indefinitely.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A technique of inputting two-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
A yellow metallic element with the atomic symbol Au, atomic number 79, and atomic weight 197. It is used in jewelry, goldplating of other metals, as currency, and in dental restoration. Many of its clinical applications, such as ANTIRHEUMATIC AGENTS, are in the form of its salts.
A scientific tool based on ULTRASONOGRAPHY and used not only for the observation of microstructure in metalwork but also in living tissue. In biomedical application, the acoustic propagation speed in normal and abnormal tissues can be quantified to distinguish their tissue elasticity and other properties.
Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)
The technique of washing tissue specimens with a concentrated solution of a heavy metal salt and letting it dry. The specimen will be covered with a very thin layer of the metal salt, being excluded in areas where an adsorbed macromolecule is present. The macromolecules allow electrons from the beam of an electron microscope to pass much more readily than the heavy metal; thus, a reversed or negative image of the molecule is created.
Proteins found in any species of bacterium.
Relating to the size of solids.
The technique of using a microtome to cut thin or ultrathin sections of tissues embedded in a supporting substance. The microtome is an instrument that hold a steel, glass or diamond knife in clamps at an angle to the blocks of prepared tissues, which it cuts in sections of equal thickness.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The process of generating three-dimensional images by electronic, photographic, or other methods. For example, three-dimensional images can be generated by assembling multiple tomographic images with the aid of a computer, while photographic 3-D images (HOLOGRAPHY) can be made by exposing film to the interference pattern created when two laser light sources shine on an object.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
An analytical transmission electron microscopy method using an electron microscope fitted with an energy filtering lens. The method is based on the principle that some of the ELECTRONS passing through the specimen will lose energy when they ionize inner shell electrons of the atoms in the specimen. The amount of energy loss is dependent upon the element. Analysis of the energy loss spectrum (ELECTRON ENERGY-LOSS SPECTROSCOPY) reveals the elemental composition of a specimen. It is used analytically and quantitatively to determine which, how much of, and where specific ELEMENTS are in a sample. For example, it is used for elemental mapping of PHOSPHORUS to trace the strands of NUCLEIC ACIDS in nucleoprotein complexes.
That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range.
The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Condensed areas of cellular material that may be bounded by a membrane.
A replica technique in which cells are frozen to a very low temperature and cracked with a knife blade to expose the interior surfaces of the cells or cell membranes. The cracked cell surfaces are then freeze-dried to expose their constituents. The surfaces are now ready for shadowing to be viewed using an electron microscope. This method differs from freeze-fracturing in that no cryoprotectant is used and, thus, allows for the sublimation of water during the freeze-drying process to etch the surfaces.
Methods of preparing tissue for examination and study of the origin, structure, function, or pathology.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
One or more layers of EPITHELIAL CELLS, supported by the basal lamina, which covers the inner or outer surfaces of the body.
The synthesis by organisms of organic chemical compounds, especially carbohydrates, from carbon dioxide using energy obtained from light rather than from the oxidation of chemical compounds. Photosynthesis comprises two separate processes: the light reactions and the dark reactions. In higher plants; GREEN ALGAE; and CYANOBACTERIA; NADPH and ATP formed by the light reactions drive the dark reactions which result in the fixation of carbon dioxide. (from Oxford Dictionary of Biochemistry and Molecular Biology, 2001)
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
An organization of cells into an organ-like structure. Organoids can be generated in culture. They are also found in certain neoplasms.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A complex of enzymes and PROTON PUMPS located on the inner membrane of the MITOCHONDRIA and in bacterial membranes. The protein complex provides energy in the form of an electrochemical gradient, which may be used by either MITOCHONDRIAL PROTON-TRANSLOCATING ATPASES or BACTERIAL PROTON-TRANSLOCATING ATPASES.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
A tissue preparation technique that involves the injecting of plastic (acrylates) into blood vessels or other hollow viscera and treating the tissue with a caustic substance. This results in a negative copy or a solid replica of the enclosed space of the tissue that is ready for viewing under a scanning electron microscope.
A flavoprotein and iron sulfur-containing oxidoreductase complex that catalyzes the conversion of UBIQUINONE to ubiquinol. In MITOCHONDRIA the complex also couples its reaction to the transport of PROTONS across the internal mitochondrial membrane. The NADH DEHYDROGENASE component of the complex can be isolated and is listed as EC
Slender, cylindrical filaments found in the cytoskeleton of plant and animal cells. They are composed of the protein TUBULIN and are influenced by TUBULIN MODULATORS.
The development and use of techniques to study physical phenomena and construct structures in the nanoscale size range or smaller.
A group of cytochromes with covalent thioether linkages between either or both of the vinyl side chains of protoheme and the protein. (Enzyme Nomenclature, 1992, p539)
The technique of using a cryostat or freezing microtome, in which the temperature is regulated to -20 degrees Celsius, to cut ultrathin frozen sections for microscopic (usually, electron microscopic) examination.
Protein complexes that take part in the process of PHOTOSYNTHESIS. They are located within the THYLAKOID MEMBRANES of plant CHLOROPLASTS and a variety of structures in more primitive organisms. There are two major complexes involved in the photosynthetic process called PHOTOSYSTEM I and PHOTOSYSTEM II.
The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.
Specific particles of membrane-bound organized living substances present in eukaryotic cells, such as the MITOCHONDRIA; the GOLGI APPARATUS; ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.
An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.
Hemeproteins whose characteristic mode of action involves transfer of reducing equivalents which are associated with a reversible change in oxidation state of the prosthetic group. Formally, this redox change involves a single-electron, reversible equilibrium between the Fe(II) and Fe(III) states of the central iron atom (From Enzyme Nomenclature, 1992, p539). The various cytochrome subclasses are organized by the type of HEME and by the wavelength range of their reduced alpha-absorption bands.
Tungsten hydroxide oxide phosphate. A white or slightly yellowish-green, slightly efflorescent crystal or crystalline powder. It is used as a reagent for alkaloids and many other nitrogen bases, for phenols, albumin, peptone, amino acids, uric acid, urea, blood, and carbohydrates. (From Merck Index, 11th ed)
An area showing altered staining behavior in the nucleus or cytoplasm of a virus-infected cell. Some inclusion bodies represent "virus factories" in which viral nucleic acid or protein is being synthesized; others are merely artifacts of fixation and staining. One example, Negri bodies, are found in the cytoplasm or processes of nerve cells in animals that have died from rabies.
Liquids transforming into solids by the removal of heat.
The study of chemical changes resulting from electrical action and electrical activity resulting from chemical changes.
Osmium. A very hard, gray, toxic, and nearly infusible metal element, atomic number 76, atomic weight 190.2, symbol Os. (From Dorland, 28th ed)
Molecules which contain an atom or a group of atoms exhibiting an unpaired electron spin that can be detected by electron spin resonance spectroscopy and can be bonded to another molecule. (McGraw-Hill Dictionary of Chemical and Technical Terms, 4th ed)
The measurement of the amplitude of the components of a complex waveform throughout the frequency range of the waveform. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.
The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The technique of placing cells or tissue in a supporting medium so that thin sections can be cut using a microtome. The medium can be paraffin wax (PARAFFIN EMBEDDING) or plastics (PLASTIC EMBEDDING) such as epoxy resins.
The sum of the weight of all the atoms in a molecule.
Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.
A large multisubunit protein complex that is found in the THYLAKOID MEMBRANE. It uses light energy derived from LIGHT-HARVESTING PROTEIN COMPLEXES to drive electron transfer reactions that result in either the reduction of NADP to NADPH or the transport of PROTONS across the membrane.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.
Discrete concentrations of energy, apparently massless elementary particles, that move at the speed of light. They are the unit or quantum of electromagnetic radiation. Photons are emitted when electrons move from one energy state to another. (From Hawley's Condensed Chemical Dictionary, 11th ed)
A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A generic term for any circumscribed mass of foreign (e.g., lead or viruses) or metabolically inactive materials (e.g., ceroid or MALLORY BODIES), within the cytoplasm or nucleus of a cell. Inclusion bodies are in cells infected with certain filtrable viruses, observed especially in nerve, epithelial, or endothelial cells. (Stedman, 25th ed)
The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.
Iron-containing proteins that transfer electrons, usually at a low potential, to flavoproteins; the iron is not present as in heme. (McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained.
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (LASER) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum.
Materials which have structured components with at least one dimension in the range of 1 to 100 nanometers. These include NANOCOMPOSITES; NANOPARTICLES; NANOTUBES; and NANOWIRES.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
A modification of the freeze-drying method in which the ice within the frozen tissue is replaced by alcohol or other solvent at a very low temperature.
The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.
The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed, e.g., a substance can be irradiated with invisible radiation and emit visible light. X-ray fluorescence is used in diagnosis.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
One of the protein CROSS-LINKING REAGENTS that is used as a disinfectant for sterilization of heat-sensitive equipment and as a laboratory reagent, especially as a fixative.
The diversion of RADIATION (thermal, electromagnetic, or nuclear) from its original path as a result of interactions or collisions with atoms, molecules, or larger particles in the atmosphere or other media. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
A lipid-soluble benzoquinone which is involved in ELECTRON TRANSPORT in mitochondrial preparations. The compound occurs in the majority of aerobic organisms, from bacteria to higher plants and animals.
Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).
Inorganic salts of the hypothetical acid, H3Fe(CN)6.
The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Porphyrin derivatives containing magnesium that act to convert light energy in photosynthetic organisms.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Inorganic or organic compounds containing trivalent iron.
Microscopy in which the image is formed by ultraviolet radiation and is displayed and recorded by means of photographic film.
Analysis of the intensity of Raman scattering of monochromatic light as a function of frequency of the scattered light.
A darkly stained mat-like EXTRACELLULAR MATRIX (ECM) that separates cell layers, such as EPITHELIUM from ENDOTHELIUM or a layer of CONNECTIVE TISSUE. The ECM layer that supports an overlying EPITHELIUM or ENDOTHELIUM is called basal lamina. Basement membrane (BM) can be formed by the fusion of either two adjacent basal laminae or a basal lamina with an adjacent reticular lamina of connective tissue. BM, composed mainly of TYPE IV COLLAGEN; glycoprotein LAMININ; and PROTEOGLYCAN, provides barriers as well as channels between interacting cell layers.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.
Any spaces or cavities within a cell. They may function in digestion, storage, secretion, or excretion.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
The testing of materials and devices, especially those used for PROSTHESES AND IMPLANTS; SUTURES; TISSUE ADHESIVES; etc., for hardness, strength, durability, safety, efficacy, and biocompatibility.
The minute vessels that connect the arterioles and venules.
A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed)
A technique for analysis of the chemical composition of molecules. A substance is bombarded with monochromatic ELECTRONS. Some of the electrons passing through the specimen will lose energy when they ionize inner shell electrons of the atoms in the specimen. The energy loss is element dependent. Analysis of the energy loss spectrum reveals the elemental composition of a specimen. ENERGY-FILTERED TRANSMISSION ELECTRON MICROSCOPY is a type of electron energy loss spectroscopy carried out in electron microscopes specially outfitted to analyze the spectrum of electron energy loss.
The first chemical element in the periodic table. It has the atomic symbol H, atomic number 1, and atomic weight [1.00784; 1.00811]. It exists, under normal conditions, as a colorless, odorless, tasteless, diatomic gas. Hydrogen ions are PROTONS. Besides the common H1 isotope, hydrogen exists as the stable isotope DEUTERIUM and the unstable, radioactive isotope TRITIUM.
The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.
Artificial, single or multilaminar vesicles (made from lecithins or other lipids) that are used for the delivery of a variety of biological molecules or molecular complexes to cells, for example, drug delivery and gene transfer. They are also used to study membranes and membrane proteins.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
The characteristic three-dimensional shape of a molecule.
The study of PHYSICAL PHENOMENA and PHYSICAL PROCESSES as applied to living things.
Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)
A representation, generally small in scale, to show the structure, construction, or appearance of something. (From Random House Unabridged Dictionary, 2d ed)
The color-furnishing portion of hemoglobin. It is found free in tissues and as the prosthetic group in many hemeproteins.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
Proteins prepared by recombinant DNA technology.
Direct contact of a cell with a neighboring cell. Most such junctions are too small to be resolved by light microscopy, but they can be visualized by conventional or freeze-fracture electron microscopy, both of which show that the interacting CELL MEMBRANE and often the underlying CYTOPLASM and the intervening EXTRACELLULAR SPACE are highly specialized in these regions. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p792)
Highly reactive molecules with an unsatisfied electron valence pair. Free radicals are produced in both normal and pathological processes. They are proven or suspected agents of tissue damage in a wide variety of circumstances including radiation, damage from environment chemicals, and aging. Natural and pharmacological prevention of free radical damage is being actively investigated.
A large multisubunit protein complex found in the THYLAKOID MEMBRANE. It uses light energy derived from LIGHT-HARVESTING PROTEIN COMPLEXES to catalyze the splitting of WATER into DIOXYGEN and of reducing equivalents of HYDROGEN.
Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS.
Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
Adherence of cells to surfaces or to other cells.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Silver. An element with the atomic symbol Ag, atomic number 47, and atomic weight 107.87. It is a soft metal that is used medically in surgical instruments, dental prostheses, and alloys. Long-continued use of silver salts can lead to a form of poisoning known as ARGYRIA.
The spectrometric analysis of fluorescent X-RAYS, i.e. X-rays emitted after bombarding matter with high energy particles such as PROTONS; ELECTRONS; or higher energy X-rays. Identification of ELEMENTS by this technique is based on the specific type of X-rays that are emitted which are characteristic of the specific elements in the material being analyzed. The characteristic X-rays are distinguished and/or quantified by either wavelength dispersive or energy dispersive methods.
Encrustations, formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedding in extracellular polymers, that adhere to surfaces such as teeth (DENTAL DEPOSITS); PROSTHESES AND IMPLANTS; and catheters. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and antifouling agents.
A group of proteins possessing only the iron-sulfur complex as the prosthetic group. These proteins participate in all major pathways of electron transport: photosynthesis, respiration, hydroxylation and bacterial hydrogen and nitrogen fixation.
Spherical phototrophic bacteria found in mud and stagnant water exposed to light.
The recording of images in three-dimensional form on a photographic film by exposing it to a laser beam reflected from the object under study.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
The physical characteristics and processes of biological systems.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
A genus of gram-negative, facultatively anaerobic rods. It is a saprophytic, marine organism which is often isolated from spoiling fish.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
An allotropic form of carbon that is used in pencils, as a lubricant, and in matches and explosives. It is obtained by mining and its dust can cause lung irritation.
Methods used to study CELLS.
Stable elementary particles having the smallest known positive charge, found in the nuclei of all elements. The proton mass is less than that of a neutron. A proton is the nucleus of the light hydrogen atom, i.e., the hydrogen ion.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
Fibers composed of MICROFILAMENT PROTEINS, which are predominately ACTIN. They are the smallest of the cytoskeletal filaments.
The study of the structure, behavior, growth, reproduction, and pathology of cells; and the function and chemistry of cellular components.
A metallic element with atomic symbol Fe, atomic number 26, and atomic weight 55.85. It is an essential constituent of HEMOGLOBINS; CYTOCHROMES; and IRON-BINDING PROTEINS. It plays a role in cellular redox reactions and in the transport of OXYGEN.
The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily.
A specialized field of physics and engineering involved in studying the behavior and properties of light and the technology of analyzing, generating, transmitting, and manipulating ELECTROMAGNETIC RADIATION in the visible, infrared, and ultraviolet range.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
Minute projections of cell membranes which greatly increase the surface area of the cell.
A heavy metal trace element with the atomic symbol Cu, atomic number 29, and atomic weight 63.55.
Antibodies produced by a single clone of cells.
An antibiotic substance produced by Streptomyces species. It inhibits mitochondrial respiration and may deplete cellular levels of ATP. Antimycin A1 has been used as a fungicide, insecticide, and miticide. (From Merck Index, 12th ed)
A family of 3,6-di(substituted-amino)-9-benzoate derivatives of xanthene that are used as dyes and as indicators for various metals; also used as fluorescent tracers in histochemistry.
A pre-emergent herbicide.
Recording serial images of a process at regular intervals spaced out over a longer period of time than the time in which the recordings will be played back.

The structlre of pili (fimbriae) of Moraxella bovis. (1/34186)

Cells from rough and smooth colonies of Moraxella bovis were examined by electron microscopy utilizing both shadowing and thin sectioning techniques. Pili were found on the surfaces of cells from rough but not smooth colonies. Pili had a peritrichoud distribution and appeared as delicate (6.5-8.5 nm in diameter), elongated unbranched filaments. When bacteria were sectioned pili did not contain central pores and appeared to originate from opacities on the surface of the cell wall.  (+info)

New perspectives on biliary atresia. (2/34186)

An investigation into the aetiology, diagnosis, and treatment of biliary atresia was carried out because the prognosis remains so poor.In an electron microscopical study no viral particles or viral inclusion bodies were seen, nor were any specific ultrastructural features observed. An animal experiment suggested that obstruction within the biliary tract of newborn rabbits could be produced by maternal intravenous injection of the bile acid lithocholic acid.A simple and atraumatic method of diagnosis was developed using(99) (m)Tc-labelled compounds which are excreted into bile. Two compounds, (99m)Tc-pyridoxylidene glutamate ((99m)Tc-PG) and (99m)Tc-dihydrothioctic acid ((99m)Tc-DHT) were first assessed in normal piglets and piglets with complete biliary obstruction. Intestinal imaging correlated with biliary tract patency, and the same correlation was found in jaundiced human adults, in whom the (99m)Tc-PG scan correctly determined biliary patency in 21 out of 24 cases. The (99m)Tc-PG scan compared well with liver biopsy and (131)I-Rose Bengal in the diagnosis of 11 infants with prolonged jaundice.A model of extrahepatic biliary atresia was developed in the newborn piglet so that different methods of bile drainage could be assessed. Priorities in biliary atresia lie in a better understanding of the aetiology and early diagnosis rather than in devising new bile drainage procedures.  (+info)

The isolation and partial characterization of the serum lipoproteins and apolipoproteins of the rainbow trout. (3/34186)

1. VLD (very-low-density), LD (low-density) and HD (high-density) lipoproteins were isolated from the serum of trout (Salmo gairdneri Richardson). 2. Each lipoprotein class resembled that of the human in immunological reactivity, electrophoretic behaviour and appearance in the electron microscope. Trout LD lipoprotein, however, was of greater density than human LD lipoprotein. 3. The trout lipoproteins have lipid compositions which are similar to those of the corresponding human components, except for their high contents of long-chain unsaturated fatty acids. 4. HD and LD lipoproteins were immunologically non-identical, whereas LD lipoproteins possessed antigenic determinants in common with VLD lipoproteins. 5. VLD and HD lipoproteins each contained at least seven different apoproteins, whereas LD liprotein was composed largely of a single apoprotein which resembled human apolipoprotein B. 6. At least one, and possibly three, apoprotein of trout HD lipoprotein showed features which resemble human apoprotein A-1.7. The broad similarity between the trout and human lipoprotein systems suggests that both arose from common ancestral genes early in evolutionary history.  (+info)

Assessment of hepatotoxic potential. (4/34186)

Philosophic concepts and pragmatic approaches toward improved understanding of the effect of drugs in the hepatocyte are reviewed. No set pattern of studies is advocated but rather observations are encouraged within the framework of studies that provide for varied exposure of the hepatocyte. Clinical usage should be imitated to provide earliest possible indications of toxicity in man. The need for definitive characterization through utilization of appropriate methodology derived from cross-fertilization of related disciplines is stressed. Both minimal and maximal dose effects should be established. Selected use of electron microscopy has become essential for characterizing responses of the liver to injury. The advantages of the toluidine blue-stained Epon "thick" sections are emphasized. Such observations are used to implement the utility of serial biopsies from the beagle dog prior to and during long-term study of potential hepatic injury. Examples of the critical effects of drug concentration within the hepatocyte are presented.  (+info)

Four dimers of lambda repressor bound to two suitably spaced pairs of lambda operators form octamers and DNA loops over large distances. (5/34186)

Transcription factors that are bound specifically to DNA often interact with each other over thousands of base pairs [1] [2]. Large DNA loops resulting from such interactions have been observed in Escherichia coli with the transcription factors deoR [3] and NtrC [4], but such interactions are not, as yet, well understood. We propose that unique protein complexes, that are not present in solution, may form specifically on DNA. Their uniqueness would make it possible for them to interact tightly and specifically with each other. We used the repressor and operators of coliphage lambda to construct a model system in which to test our proposition. lambda repressor is a dimer at physiological concentrations, but forms tetramers and octamers at a hundredfold higher concentration. We predict that two lambda repressor dimers form a tetramer in vitro when bound to two lambda operators spaced 24 bp apart and that two such tetramers interact to form an octamer. We examined, in vitro, relaxed circular plasmid DNA in which such operator pairs were separated by 2,850 bp and 2,470 bp. Of these molecules, 29% formed loops as seen by electron microscopy (EM). The loop increased the tightness of binding of lambda repressor to lambda operator. Consequently, repression of the lambda PR promoter in vivo was increased fourfold by the presence of a second pair of lambda operators, separated by a distance of 3,600 bp.  (+info)

Difference between mammary epithelial cells from mature virgin and primiparous mice. (6/34186)

Mammary epithelial cells from mature virgin mice are similar to those from primiparous mice in several respects. However, there is one known difference. The cells from the mature virgin must traverse the cell cycle in order to become competent to make casein and enzymatically active alpha-lactalbumin in vitro; those from the primiparous animal can make these proteins without first traversing the cycle. In this regard, cells from human placental lactogen- and prolactin-treated mature virgins are, after involution, similar to those from primiparous mice. The developemental block in the cells from the mature virgin, imposed by preventing cell cycle traversal, has been partially delineated. It does not appear to reside at the levels of ultrastructural maturation or the formation of casein messenger RNA. Rather, the lesion is postranscriptional and may be at the level of translation, or posttranslational modification, or both.  (+info)

Unsaturated fatty acid requirements for growth and survival of a rat mammary tumor cell line. (7/34186)

A cell line, the growth and survival of which is markedly affected by linoleic acid, has been established from a carcinogen-induced rat mammary tumor. The cells have been continuously passaged in 5% rat serum plus 10% fetal calf serum-supplemented medium. The rat serum component was found to be indispensalbe, for when it was omitted the growth rate rapidly declined and the cells died by 5 to 7 days. Removal of the rat serum from the growth medium also resulted in a dramatic loss of Oil Red O-positive droplets in the cells, suggesting that the lipid component of rat serum might be a major growth-promoting principle in rat serum. This is likely since the total lipid fraction, but not the delipidized protein fraction, could largely supplant requirement of the cells for rat serum. Pure linoleic acid was found to be effective in maintaining the cell growth in delipidized serum or in whole fetal calf serum-supplemented medium. Fatty acid analysis revealed a 19-fold higher amount of linoleic acid in rat serum than in fetal calf serum.  (+info)

Effect of desiccation on the ultrastructural appearances of Acinetobacter baumannii and Acinetobacter lwoffii. (8/34186)

An Acinetobacter baumannii isolate survived desiccation beyond 30 days and an Acinetobacter lwoffii isolate up to 21 days. For both species, desiccation resulted in a significant increase in the proportion of round cells (A baumannii, 40% to 80%; A lwoffii, 51% to 63%) and a significant decrease in rod shaped cells (A baumannii, 58% to 13%; A lwoffii, 46% to 34%). Electronmicroscopic examination showed that there was also a corresponding significant increase in the cell wall thickness (A baumannii, up to 53%; A lwoffii, up to 26%). Desiccated A baumannii cells became more electron-dense and had significantly thicker cell walls (x1.3) than those of A lwoffii. Cell wall structures of A baumannii strains with different abilities to resist desiccation deserve further study.  (+info)

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We have developed techniques for studying patch-clamped membranes inside glass pipettes using high voltage electron microscopy (HVEM). To preserve the patch structure with the least possible distortion, we rapidly froze and freeze dried the pipette tip. The pipette is transparent for more than 50 microns from the tip. HVEM images of patches confirm light microscopy observations that the patch is not a bare bilayer, but a membrane-covered bleb of cytoplasm that may include organelles and cytoskeleton. The membrane that spans the pipette is commonly tens of micrometers from the tip of the pipette and occasionally as far as 100 microns. The structure of patches taken from a single cell type is variable but there are consistent differences between patches made from different cell types. With suction applied to the pipette before seal formation, we have seen in the light microscope vesicles swept from the plasmalemma up the pipette. These vesicles are visible in electron micrographs, particularly ...
The structure and distribution of cytoplasmic membranes during mitosis and cytokinesis in maize root tip meristematic cells was investigated by low and high voltage electron microscopy. The electron opacity of the nuclear envelope and endoplasmic reticulum (ER) was enhanced by staining the tissue in …
TY - JOUR. T1 - Solid state amorphization of metastable Al0.5TiZrPdCuNi high entropy alloy investigated by high voltage electron microscopy. AU - Nagase, Takeshi. AU - Takeuchi, Akira. AU - Amiya, Kenji. AU - Egami, Takeshi. PY - 2018/5/1. Y1 - 2018/5/1. N2 - The phase stability of high entropy alloy (HEA), Al0.5TiZrPdCuNi, under fast electron irradiation was studied by in-situ high voltage electron microscopy (HVEM). The initial phase of this alloy quenched from the melt was dependent on cooling rate. At high cooling rates an amorphous phase was obtained, whereas a body-centered cubic (b.c.c.) phase were obtained at low cooling rates. By thermal crystallization of the amorphous phase b.c.c. phase nano-crystals were formed. Upon fast electron irradiation solid state amorphization (SSA) was observed in b.c.c. phase regardless of the initial microstructure (i.e., coarse crystalline structure or nano-crystalline structure with grain boundaries as a sink for point defects). SSA behavior in the ...
TY - JOUR. T1 - Quantitative Electron Microscopic Investigation on Changes of Mitochondria in Long‐Term CPZ Administration in Rat Brain, Liver and Heart. AU - Takeichi, Masashi. AU - Sato, Takeshi. PY - 1987/12. Y1 - 1987/12. N2 - Abstract: The effect of chronic administration of chlorpromazine (CPZ) on mitochondria (MT) in the rat brain (striatum), liver and heart was quantitatively examined with both the electron microscope and computer‐assisted PICTURE ANALYSIS OA‐1. The specimens were taken from six female rats, three controls and three experimentals to which 20 mg/kg body weight of CPZ was administered orally for 15 months from 5 weeks after birth. Thus the quantitative electron microscopic study was made on the mitochondrial sizes (cross‐sectional areas) and cristal densities and the following results were obtained: 1) The sizes of MT from the striatal nerve cells and axo‐dendritic spine postsynapses and liver parenchymal cells were larger in the CPZ‐administered rats than in ...
1963 Conference on Electron Microscopy, Gliwice, chairman: S. Gorczyca, in Polish, 10 papers (unpublished) 1971 II Polish Conference on Electron
Electron microscopic localization of acetylcholinesterase in the superior cervical ganglion of the rat. In: Kolligátum. pp. 274-285. (1967 ...
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TY - JOUR. T1 - A structural study of tin-antimony oxide catalysts by high-resolution electron microscopy. AU - Berry, Frank J.. AU - Smith, David J.. PY - 1984/7. Y1 - 1984/7. N2 - Tin-antimony oxide catalysts prepared by the calcination of precipitates have been investigated by high-resolution electron microscopy. The exposure of the catalysts prepared at low temperatures to gaseous atmospheres containing hydrocabon and oxygen results in a segregation of antimony from the poorly crystalline rutile-type solids and the development of an amorphous material. The catalysts containing low concentrations of antimony are also partially converted to a non-rutile-type crystalline phase. Prolonged calcination in air of the used catalysts at high temperatures leads to the attainment of bulk equilibrium and the formation of solid solutions of antimony in tin(IV) oxide. Treatment of the equilibrated crystalline catalysts prepared at high temperatures in the hydrocarbon and oxygen gas stream gives rise to ...
A method for forming a zoned distribution of particulate material within a fibrous web includes a conveying step for providing a gas entrained supply of the particulate material and a segregating step centrifugally directing at least a portion of the particulate material into an accumulation region. A transferring step selectively directs particulate material from the accumulation region into a delivery gas stream to provide an intermittent flow volume of a selected quantity of particulate material from the accumulation region through a delivery conduit and into a web forming chamber. A fiberizing step provides a flow of a selected fibrous material into the web forming chamber, and a directing step controls the intermittent flow of particulate material from the delivery conduit into the forming chamber. A foraminous forming layer is disposed within the forming chamber for receiving the fibrous material and the particulate material to produce a fibrous web which includes zoned regions having selected,
Mitosis in yeast Saccharomyces cerevisiae was investigated in thick (0-25-I mum) serial sections with a high voltage electron microscope and in preparations of spheroplasts spread on a water surface. Spindle microtubules originate from a plaque-like structure called the spindle pole bosis the SPB duplicates and a set of long and short microtubules develops on each SPB. The spindle arises as the SPBs separate on the nuclear membrane adense and are not individually visible. Genetic studies, however, have indicated that there are 17 linkage groups. The number of microtubules was determined in diploid and haploid spindles on serial stereo micrographs. In diploid mitosis about 40 microtubules issue from a SPB. Most are non-continuous and often they are visibly associated with a chromatin fibre. The spindle in haploid cells is similar except that the number of microtubules is about half that in diploid cells and the SPB is smaller. The pole-to-pole microtubules vary in number from spindle to spindle, ...
TY - JOUR. T1 - The structure of grain boundaries in granite-origin ultramylonite studied by high-resolution electron microscopy. AU - Hiraga, T.. AU - Nagase, Toshiro. AU - Akizuki, M.. PY - 1999/9/1. Y1 - 1999/9/1. N2 - The structure of grain boundaries in a granite-origin ultramylonite, composed mainly of fine-grained feldspar and quartz, was studied by high-resolution electron microscopy (HREM). At most of the boundaries, not only between the same minerals but also between different minerals, lattice fringes in adjacent grains meet at the interface with no other appreciable phases. In these boundaries, some of the straight segments correspond to a low-index plane of one of the connected grains. Boundaries containing voids, with a spheroidal shape elongated along the boundaries, were observed only between quartz grains. It is suggested that these boundaries were formed by healing of microcracks. The structural width of major boundaries, deduced from lattice-fringe imaging, is less than about ...
The Acanthamoeba castellanii myosin-Is were the first unconventional myosins to be discovered, and the myosin-I class has since been found to be one of the more diverse and abundant classes of the myosin superfamily. We used two-dimensional (2D) crystallization on phospholipid monolayers and negative stain electron microscopy to calculate a projection map of a classical myosin-I, Acanthamoeba myosin-IB (MIB), at ∼18 Å resolution. Interpretation of the projection map suggests that the MIB molecules sit upright on the membrane. We also used cryoelectron microscopy and helical image analysis to determine the three-dimensional structure of actin filaments decorated with unphosphorylated (inactive) MIB. The catalytic domain is similar to that of other myosins, whereas the large carboxy-terminal tail domain differs greatly from brush border myosin-I (BBM-I), another member of the myosin-I class. These differences may be relevant to the distinct cellular functions of these two types of myosin-I. ...
Biology is a challenging and complicated mess. Understanding this challenging complexity is the realm of the biological sciences: Trying to make sense of the massive, messy data in terms of discovering patterns and revealing its underlying general rules. Among the most powerful mathematical tools for organizing and helping to structure complex, heterogeneous and noisy data are the tools provided by multivariate statistical analysis (MSA) approaches. These eigenvector/eigenvalue data-compression approaches were first introduced to electron microscopy (EM) in 1980 to help sort out different views of macromolecules in a micrograph. After 35 years of continuous use and developments, new MSA applications are still being proposed regularly. The speed of computing has increased dramatically in the decades since their first use in electron microscopy. However, we have also seen a possibly even more rapid increase in the size and complexity of the EM data sets to be studied. MSA computations had thus become a
A hemagglutination-inhibitory mucoprotein from human urine has been studied with the electron microscope. It consists of filaments, with diameters of 40 to , 240 A, composed of smaller fibrils. In the two-dimensional projection of the electron micrographs, the single fibrils often show a zig-zag course with a periodicity of 100 to 140 A; the single branch of a zig-zag measures about 60 A in length and either 20 or 40 A in width. Still thinner fibrillar elements are observable with diameters of 10 A or less. In three-dimensional aspect, the zig-zag structure might be a helix. The fibril-bundle (or filament) reveals a complicated configuration. Heat treatment at 70°C shows some indication of denaturation (e.g. filaments are shorter), whereas at 80°C almost complete degradation of the protein into individual zig-zag elements or smaller pieces is attained. The interaction between influenza virus particles and inhibitory mucoprotein consists of the attachment of a fiber molecule to the virus ...
EMDB EMD-22958: Negative stain electron microscopy reconstruction of 2P SARS-CoV-2 spike ectodomain in complex with Fabs DH1047 and DH1051
Microplastics collected at sea harbour a high diversity of microorganisms including some Vibrio genus members, raising questions about the role of microplastics as a novel ecological niche for...
Fibrinogen conjugated to colloidal gold or colloidal gold-monoclonal anti-glycoprotein IIb/IIIa (fibrinogen receptor) was used to label the receptor on platelets. Whole mount preparations were examined by stereo pair high voltage electron microscopy and then by scanning electron microscopy to determine the feasibility of this approach in detecting the number of receptors and their location relative to the cytoskeletal and surface structure. Both the ligand-gold and antibody-gold labels were effective. The relative numbers of receptors could be seen and their relationship to cytoskeletal structure could be determined. Marked differences in receptor number and distribution were observed when platelets in different stages of activation were compared. In co-cultured macrophages and platelets, receptors were found exclusively on platelets or on pieces of platelet membrane adherent to macrophages.
The fusion of lysosomes to phagosomes was observed under high voltage electron microscopy, in 4μm thick rat retinal sections with the aid of acid phosphatase cytochemistry. The study of thick sections facilitates the observation of the moment of fusion in stereo view from two tilted pictures. From this study, the contents of the lysosome pored into the phagosome through the orifice, shortly after the collision of the two organelles. The hydrolytic enzymes such as acid phosphatase spread in a sheet under the limiting membrane of the phagosome to finally form a balloon of the reaction product. In some case the ballooning appeared to be doubled. The outer skin of the reaction product may be the result of a wrapping mechanism of phagolysosomes.. ...
Time-lapse images of particulate matter (PM) deposition on diesel particulate filters (DPFs) at the PM-particle scale were obtained via field-emission scanning electron microscopy (FE-SEM). This particle scale time-series visualization showed the detailed processes of PM accumulation inside the DPF. First, PM introduced into a micro-pore of the DPF wall was deposited onto the surface of SiC grains composing the DPF, where it formed dendritic structures. The dendrite structures were locally grown at the contracted flow area between the SiC grains by accumulation of PM, ultimately constructing a bridge and closing the porous channel. To investigate the dominant parameters governing bridge formation, the filtration efficiency by Brownian diffusion and by interception obtained using theoretical filtration efficiency analysis of a spherical collector model were compared with the visualization results. The initial deposition of PM on the SiC grains showed good agreement with theoretical observations, ...
TY - JOUR. T1 - Anatomical and physiological observations on synapses formed on isolated autonomic neurons in tissue culture. AU - Bunge, Richard P.. AU - Rees, Rosemary. AU - Wood, Patrick. AU - Burton, Harold. AU - Ko, Chien Ping. N1 - Funding Information: We benefited from the opportunity to discuss the development of this system with Drs. Dennis Bray, Stanley Crain and Robert Porter. Supported by NIH Grants NS-09923 and NS-09809 and GRS-5 SO1 RR05398.. PY - 1974/2/8. Y1 - 1974/2/8. N2 - Neurons from the superior cervical ganglion of perinatal rats were freed of their supporting cells and established in culture by the method of Bray. To these were added meninges-free explants of embryonic rat thoracic spinal cord to allow interaction between the outgrowing cord neurites and the isolated autonomic neurons. Electron microscopic observations on the autonomic neurons in these preparations revealed the presence of axon terminals of two types. One type (presumably adrenergic) contained pleomorphic ...
Even small amounts of amorphous materials can have a significant effect on the drug product. Are gravimetric vapour sorption techniques an effective solution to characterize amorphous materials?
Methodology to annotate the multiple origins of axonal projections in dense electron microscopy data of mammalian nervous tissue without the need of chemical label conversion is reported.
A light-and electron-microscopic study of pig hepatocytes from late prenatal to early neonatal animals shows changes which reflect an increasing rate of synthetic activity. The granular endoplasmic reticulum (ER) in the prenatal pig hepatocyte is situated along the periphery of the cytoplasm and in the region immediately surrounding the nucleus. Mitochondria are most abundant in the area adjacent to the nucleus, while the Golgi complex is generally located in the region of the bile canaliculus. The remaining portion of the hepatocyte is occupied with glycogen. A few hours after birth the hepatocyte increases about twofold in size with the nucleus shifting from a peripheral to a more centrally located position. The glycogen decreases quickly coincident with a rapid increase in the amount of granular ER and the dispersion of the mitochondria throughout the cell. The Golgi complex becomes distended and numerous vesicles appear in its immediate vicinity containing a moderately dense material. ...
Out of 54 open renal biopsies performed on children, 17 were selected and studied not only by light microscopy but also by electron microscopy. Statistical-mathematical analysis of basement membrane thickness-measurements was carried out. It is concluded that electron microscopic investigation of the renal biopsy material is required in selected cases involving diagnostic or therapeutic problems. In nephrotic syndrome it may help above all to separate minimal changes from early forms or glomerular lesion of the focal sclerosing type. Electron microscopy can also be useful in any of the mono- and oligosymptomatic renal diseases. In those cases only electron microscopy makes it possible to provide exact morphological diagnosis which is necessary to the proper therapy. ...
Partial denaturation pattern of sex factor deoxyribonucleic acid of Escherichia coli was studied by electron microscopy. Clustering of the adenine-plusthymine-rich regions in one part of the molecule was revealed. The positions of these regions were located on the physical map of F by analyzing the partial denaturation pattern of heteroduplexes between F and F-prime factors with various parts of F sequences deleted. ...
TY - CHAP. T1 - Correlative light and electron microscopy of autophagosomes. AU - Gudmundsson, Sigurdur Runar. AU - Kahlhofer, Jenny. AU - Baylac, Nastassia. AU - Kallio, Katri Anneli. AU - Eskelinen, Eeva-Liisa. PY - 2019/1. Y1 - 2019/1. N2 - Live-cell imaging has been widely used to study autophagosome biogenesis and maturation. When combined with correlative electron microscopy, this approach can be extended to reveal ultrastructural details in three dimensions. The resolution of electron microscopy is needed when membrane contact sites and tubular connections between organelles are studied.. AB - Live-cell imaging has been widely used to study autophagosome biogenesis and maturation. When combined with correlative electron microscopy, this approach can be extended to reveal ultrastructural details in three dimensions. The resolution of electron microscopy is needed when membrane contact sites and tubular connections between organelles are studied.. KW - 1182 Biochemistry, cell and molecular ...
Hi, I am Stefan Fischer. I am biologist at the University of Tübingen, working in the department for Evolutionary Biology of Invertebrates (Institute of Evolution and Ecology). My methodological focus is set on electron microscopy. Furthermore I do focus on 3D techniques, be it on basis of semi-thin sections (LM), Ultra-thin sections (ssTEM) and also MicroCT data. For that reason I developed a strong interest in open source software packages and image analysis in order to automate (or semi-automate) some of the tedious parts of my workflows.. ...
Conventional electron microscopy offers a substantial resolution advantage over light microscopy, but requires difficult and often destructive preparation techniques. Recent advances in electron microscopy allow for imaging of hydrated samples, retaining the resolution advantage while removing the difficulty in preparation. Two new techniques, environmental scanning electron microscopy and wet electron microscopy offer this advantage, allowing for new possibilities in biological imaging.
The fine structure of the His bundle is described on the basis of its light and electron microscopic appearance. Electron microscopy was performed on one human and two canine hearts, and light microscopy on over 400 human and 60 canine hearts. The His bundle was identified by its light microscopic appearance. There were no significant differences in the fine structure of human and canine His bundles. In both, the principal cell was a typical Purkinje cell containing few myofibrils and a large perinuclear clear zone; these cells are shorter and broader than working myocardial cells, and their intercellular junctions (which are obliquely rather than transversely oriented) contain a high proportion of nexus formations. Both the human and canine His bundles are partitioned by fine collagen septa, which are longitudinally oriented with comparatively few crossover connections. The general organization of the His bundle is thus into multiple strands of Purkinje cells, and these strands are largely ...
With the use of the method described in the preceding paper (to be referred to subsequently as I) for constructing the displacement fields, the electron microscope image contrast of small dislocation loops and of stacking-fault tetrahedra has been computed from numerical solutions of the Howie-Whelan (1961) equations. The computer-simulated images, displayed in the form of half-tone pictures, have been used to identify the nature and geometry of such defects in ion-irradiated foils. A systematic study of the contrast of small Frank loops in Cu+ ion irradiated copper under a wide variety of diffraction conditions is reported. In particular the variations of the contrast of loops edge-on and inclined to the electron beam with the operating Bragg reflexion, the thickness and inclination of the foil, depth of the defect in the foil and deviation from the Bragg-reflecting condition have been studied. Methods of obtaining useful information, such as the diameters of the loops, are suggested. The ...
A new type of Sr-containing sialon polytypoid phase with the structural formula SrSi10-xAl18+xN32-xOx (x approximate to l) has been found in the Sr-Si-Al-O-N system. The phase was characterised by X-ray powder diffraction (XRPD), and its structure was investigated by electron diffraction (ED) and high resolution electron microscopy (HREM). It is considerably disordered, but the average structure has a rhombohedral unit cell with a=5.335(5)approximate to root 3.a(AIN) and c= 79.1(1)Angstrom approximate to 30.c(AIN). The Sr atoms ave located in layers M-Sr-M, M=(Si/Al), at the origin of the unit cell with 12 X= (O,N) atoms around it, at distances of similar to 3 Angstrom, forming a cubo-octahedron. The X atoms that form a hexagon around the Sr atom in the ab plane are corner shared by M = (Si/Al) tetrahedra with opposite polarity in adjacent layers in which 2/3 of the tetrahedra are occupied. The M-Sr-M layers alternate with normally eight-layer-thick AIN type blocks, although the thickness of ...
The antimalarial drug, artemisinin packs a double whammy -- exploding inside malaria parasites and shutting down the waste disposal system that deals with the damage. Resistance to artemisinin, can be overcome by co-treating parasites with an anti-cancer drug of the proteasome-inhibitor class. Electron microscope images of malaria parasites (blue) generated by Associate Professor Eric Hanssen, University of Melbourne, Australia, and chemical structure of artemisinin.
RICHMOND, Va., June 7 /PRNewswire-USNewswire/ -- National Environmental Testing Lab Now IDs Carbon Black with Electron-Microscopy.
Learn how your ultrastructural investigations can benefit from modern electron microscope techniques. Correlative microscopy provides you deeper insights about cell structure by combination of light and scanning electron microscopy methods.
TY - JOUR. T1 - The mechanism of vortex connection at a free surface. AU - Zhang, Chiong. AU - Shen, Lian. AU - Yue, Dick K.P.. PY - 1999/4/10. Y1 - 1999/4/10. N2 - Vortex connections al the surface are fundamental and proouncnc features to free-surface vortical flows. To understand the detailed mechanism of such connection, we consider, as a canonical problem, the laminar vortex connections at a free surface when an oblique vortex ring impinges upon thai surface. We perform numerical simulations of the Navier Stokes equations with viscous free-surface boundary conditions. It is found that the key to understanding the mechanism of vortex connection at a free surface is the surface layers: a viscous layer resulting from the dynamic zero-stress boundary conditions at the free surface, and a thicker blockage layer which is due to the kinematic boundary condition at the surface. In the blockage layer, the vertical vorticity component increases due to vortex stretching and vortex turning (from the ...
Cryoelectron microscopy of biological molecules is among the hottest growth areas in biophysics & structural biology at present.Frank, Joachim is the author of Three-Dimensional Electron Microscopy Of Macromolecular Assemblies Visualization Of Biological Molecules In Their Native State, published 2006 under ISBN 9780195182187 and ISBN 0195182189. [read more] ...
TY - JOUR. T1 - Oxidation of bismuth-tungsten bronzes. AU - Jefferson, D. A.. AU - Uppal, M. K.. AU - Smith, David J.. PY - 1984/6. Y1 - 1984/6. N2 - The oxidation of bismuth-tungsten bronzes at 600 and 950°C has been studied using high-resolution electron microscopy at 200 and 500 kV. At the lower temperature, a topotactic transformation to lamellae of Bi2WO6 in a WO3 matrix was observed but at higher temperature larger crystals were produced, primarily of Bi2W2O9 but with some disordered intergrowths.. AB - The oxidation of bismuth-tungsten bronzes at 600 and 950°C has been studied using high-resolution electron microscopy at 200 and 500 kV. At the lower temperature, a topotactic transformation to lamellae of Bi2WO6 in a WO3 matrix was observed but at higher temperature larger crystals were produced, primarily of Bi2W2O9 but with some disordered intergrowths.. UR - http://www.scopus.com/inward/record.url?scp=0021442349&partnerID=8YFLogxK. UR - ...
Results The LVP consists of a broad nucleocapsid surrounding an electron-dense centre, presumably containing the HCV genome. The nucleocapsid is surrounded by an irregular, detergent-sensitive crescent probably composed of lipids. Lipid content may determine particle size. These particles carry HCV E1E2, ApoB and ApoE, as shown in our immuno-EM analysis. Our results also suggest that these putative LVPs circulate in the serum of patients as part of a mixed population, including lipoprotein-like particles and complete viral particles. ...
Results The LVP consists of a broad nucleocapsid surrounding an electron-dense centre, presumably containing the HCV genome. The nucleocapsid is surrounded by an irregular, detergent-sensitive crescent probably composed of lipids. Lipid content may determine particle size. These particles carry HCV E1E2, ApoB and ApoE, as shown in our immuno-EM analysis. Our results also suggest that these putative LVPs circulate in the serum of patients as part of a mixed population, including lipoprotein-like particles and complete viral particles. ...
HIGH-RESOLUTION ELECTRON C - 9780195042757 By Buseck: Buy its Hardcover Edition at lowest price online for Rs 3318 at BuyHatke.com.
With the resolution becoming sufficient to reveal individual atoms, HREM is now entering the stage where it can compete with X-ray methods to quantitatively determine atomic structures of materials without much prior knowledge, but with the advantage of being applicable to aperiodic objects such as crystal defects. In our view the future electron microscope will be characterised by a large versatility in experimental settings under computer control such as the illumination conditions (TEM-STEM), CBED, detecting conditions (diffraction, image, ptychography) and many other tunable parameters such as focus (g), voltage, spherical aberration (C-s), beam tilt, etc. Since modem detectors can detect single electrons, also the counting statistics is known. The only limiting factor in the experiment will be the total number of electrons that interact with the object during the experiment due to the limitations in the exposure time or in the object damage. However, instrumental potentialities will never ...
Transmission electron micrographs showing the tracheal epithelium in Ig-deficient mice. The tracheal epithelium in SAL/SAL-treated animals (a) harbored only
An electron microscopic investigation was performed on 28 Clostridium difficile strains isolated from 15 antibiotic-associated diarrhea cases and from 13 healthy infants. Through the use of supernatants of the cultures induced by mitomycin C (1 or 3 micrograms/ml), 18 of the 28 C. difficile strains …
Free Public Domain Picture: Here a negative stain electron micrograph reveals a M (mulberry type) monkeypox virus virion in human vesicular fluid. See PHIL 10817 for | ID: 13540024213919
Schulte, E., 1972: Electron microscopic studies on renal tubules (Malpighian tubules) in Drosophila melanogaster. V. Localization of Na-adenosine triphosphatase
The THO complex participates during eukaryotic mRNA biogenesis in coupling transcription to formation and nuclear export of translation-competent messenger ribonucleoprotein particles. In Saccharomyces cerevisiae, THO has been defined as a heteropentamer composed of the Tho2p, Hpr1p, Tex1p, Mft1p, and Thp2p subunits and the overall three-dimensional shape of the complex has been established by negative stain electron microscopy. Here, we use small-angle X-ray scattering measured for isolated THO components (Mft1p and Thp2p) as well as THO subcomplexes (Mft1p-Thp2p and Mft1p-Thp2p-Tho2p) to construct structural building blocks that allow positioning of each subunit within the complex. To accomplish this, the individual envelopes determined for Mft1p and Thp2p are first fitted inside those of the Mft1p-Thp2p and Mft1p-Thp2p-Tho2p complexes. Next, the ternary complex structure is placed in the context of the five-component electron microscopy structure. Our model reveals not only the position of ...
TY - JOUR. T1 - Efficient three-dimensional reconstruction of synapse with high-voltage electron microscopy. AU - Kea, Joo Lee. AU - Park, Chang Hyun. AU - Rhyu, Im Joo. N1 - Funding Information: The authors would like to thank Dr Kiyoshi Hama for helpful comments on the manuscript. We appreciate the strong support of the HVEM group at KBSI, Dae-Jeon, South Korea. This work was supported by Brain Korea 21 Project for Biomedical Science and grant from the Korean Health 21 R&D Project, Korean Ministry of Health and Welfare (HMP-00-GN-01-0002).. PY - 2005/4. Y1 - 2005/4. N2 - Three-dimensional (3-D) information on nervous tissue is essential for the understanding of brain function. Especially, 3-D synaptic analyses on serial ultrathin sections with transmission electron microscopy (TEM) have contributed to the knowledge on neural plasticity associated with various pathophysiological conditions. The 3-D reconstruction procedures, however, not only require a great amount of expertise but also include ...
Electron microscopic observations on normally differentiating and α-MSH (melanocytestimulating hormone)-treated epidermal melanocytes of newborn mouse skin were carried out. The process of melanocyte differentiation from premelanosome-containing melanoblasts was investigated in detail with respect to melanosomes as markers.. Melanoblasts containing unmelanized premelanosomes gradually decreased in number after birth, while the number of melanocytes rapidly increased. The epidermis of α-MSH-treated 3-day-old mice and normal 6-day-old mice contained melanocytes with numerous fully melanized melanosomes, and with no or only a few melanoblasts.. Changes in other organelles in differentiating melanocytes were also noticeable. Golgi apparatus and RER (rough endoplasmic reticulum) decreased in number during the normal or α-MSH-induced differentiation of the epidermal melanocytes, though the number of mitochondria showed no notable change. The number of SER (smooth endoplasmic reticulum) per cell did ...
The effect of 0.5 mM cadmium (Cd) was studied on the ultrastructural aspects and pectin features of the walls of flax cellulosic fibres when the thickening of secondary wall had just started in the hypocotyl of 10-day old seedlings. As seen by PATAg staining in controls, cell-wall formation displayed two distinct steps, secretion and remodelling, which did not occur simultaneously for all the neighbouring fibres. The inner part of the secondary wall, where the cellulose molecules had just been synthesized, appeared very reactive to PATAg. The outer part, where the cellulose fibrils associated in larger microfibril complexes, became non-reactive to PATAg. Under Cd treatment, we noticed some acceleration of fibre differentiation in terms of fibre number, wall thickness and yield. As revealed by PATAg staining, treated fibres exhibited a disturbed cell-wall texture, indicating a modified adhesion between the matrix polysaccharides and the cellulose microfibrils. The Cd impact on the distribution of highly
The non-clay and clay mineralogy of the British argillaceous sedimentary rocks are discussed. The non-clay mineralogy is only considered in general terms due to the lack of detailed information. The general nature of the clay minerals, their genesis and the effects of burial diagenesis on clay minerals are reviewed. This is followed by a more detailed examination of the clay mineralogy of the Palaeozoic, Mesozoic and Tertiary argillaceous rocks. Consideration is given to the relative importance of the depositional environment and burial diagenesis in forming the clay assemblages now found in British mudrocks.. The petrology of the argillaceous sedimentary rocks is discussed in general terms. The use of electron microscopy in the examination of mudrocks generally and some British examples in particular is reviewed, including a discussion of the nature and origin of fissility. The possibility of using high voltage electron microscopy in future studies of argillaceous sediments is proposed based on ...
Human lenses extracted for cataract 26 years after long-term exposure to an imperfectly shielded radium source were examined by slit-lamp photography, thin-section light microscopy, and electron microscopy. Anterior epithelial cells were fibroblast-like, and germinal epithelium and vacuolated cortical fibres had accumulated at the equator. A zone of light scatter at the anterior pole corresponded to an area of breakdown of cortical lens fibres, where unusual feathery fibres were orientated perpendicular to the lens surface. Two zones of light scatter separated by a 250-microM clear interval were seen in the posterior cortex. The zone at the posterior pole corresponded to an area of fibre liquefaction and large rounded membrane whorls, while the deeper zone comprised small flattened membrane whorls. The characteristic plaques of swollen abnormal cells described in previous histological studies of x-ray cataract were not present. This and other differences probably reflect the extremely long time ...
1. 1. At the growth temperature the total phospholipids isolated from Escherichia coli cells give rise to 31P-NMR spectra which indicate the existence of lamellar, isotropic and hexagonal phases. These phases are also detected by freeze-fracture electron microscopy. In particular, the isotropic phase may contain lipidic particles (possibly inverted micelles) ... read more associated with the lamellar phase. 2. 2. The cytoplasmic membrane isolated from E. coli cells grown at 37°C is mainly lamellar at 25°C, whereas at 37 and 45°C the presence of some almost isotropic phospholipid motion is indicated. The possible significance of the isotropic phase for the functioning of the cytoplasmic membrane is discussed. show less ...
The adult lobster Homarus gammarus is a weak hyper-regulator at low salinity. The objective of this study was to locate the ion-transporting tissues in the branchial chamber of this species, using electron microscopy and confocal laser scanning microscopy with a fluorescent vital stain for mitochondria, DASPMI, which is widely used to locate mitochondria-rich cells in ion-transporting epithelia of fish. A thick mitochondria-rich epithelium is present on the inner side of the branchiostegite and over the entire surface of the epipodites. Ultrastructural observations confirm that this tissue has features typical of an ion-transporting epithelium. When the lobster is transferred to low salinity, these epithelia undergo marked ultrastructural changes, such as an increase in thickness related to the development of basolateral infoldings, the appearance of numerous vesicles and an increase in height of the apical microvilli. In the gills, the branchial filaments are lined by a thin and poorly ...
EVP is an associate professor of computer systems department in School of Natural Sciences in Far Eastern Federal University. He has a Ph.D. in Physics and great experience in electron microscopy. His scientific interests are electron microscopy, physics of condensed matter, image processing, and high-performance computations on GPU. EBM is currently a Ph.D. student of School of Natural Sciences in Far Eastern Federal University. His Ph.D. project focuses on electron microscopy of amorphous and nanocrystalline metallic alloys and their structure changes under external impact. OVV is a Ph.D. student of School of Natural Sciences in Far Eastern Federal University. His Ph.D. project focuses on electron microscopy and electron tomography of structure inhomogeneities in amorphous metallic alloys.ANF holds a BS degree in Information Systems from Far Eastern Federal University. He is currently working toward a masters degree in Information Systems and Technologies at Far Eastern Federal University. He ...
Due to its actin-sequestering properties, thymosin beta-4 (Tβ4) is considered to play a significant role in the cellular metabolism. Several physiological properties of Tβ4 have been reported;, however, many questions concerning its cellular function remain to be ascertained. To better understand the role of this small peptide we have analyzed by means of transmission immunoelectron microscopy techniques the ultrastructural localization of Tβ4 in HepG2 cells. Samples of HepG2 cells were fixed in a mixture of 3% formaldehyde and 0.1% glutaraldehyde in 0.1 M cacodylate buffer and processed for standard electron microscopic techniques. The samples were dehydrated in a cold graded methanol series and embedded in LR gold resin. Ultrathin sections were labeled with rabbit antibodies to Tβ4, followed by gold-labeled goat anti-rabbit, stained with uranyl acetate and bismuth subnitrate, observed and photographed in a JEOL 100S transmission electron microscope. High-resolution electron microscopy ...
After monolayer cultures of rat islets were exposed to [125I]insulin,[125I]glucagon, and [125I]tyrosinyl somatostatin, specific autoradiographic grains associated with each radioactively labeled ligand were found on B, A, and D cells.The density of labeling of the B, A, and D cells with each labeled ligand correlated well with the known actions of the three hormones on each of the islet cells. ...
Ice crystal larger than about 5 μm diameter were separated from interstitial particles in aircraft contrails and evaporated. Residual particles larger than 0.1 μm were analyzed by electron microscopy. Soot, metals, and volatile organic substances, apparently from the aircraft exhaust, were found. However, the residual particles also contained high percentages of minerals, thought to be crustal in origin, that were often mixed with sulfur. The percentages of particles in our samples (representing the larger residual particles from relatively large ice crystals) identified as exhaust-derived and the percentage apparently derived from the ambient aerosol were roughly equal, suggesting that ambient particles may be important in contrail formation. Possible explanations for this are presented ...
TY - ABST. T1 - Fe-Modeling Of Starved Hydrodynamic Lubrication With Free Surface Effects. AU - Poulios, Konstantinos. AU - Vølund, Anders. AU - Klit, Peder. PY - 2017. Y1 - 2017. N2 - This work concerns a new finite-element formulation for solving hydrody-namic lubrication problems that include partially flooded regions, where the lubricantfilm behavior is governed by free surface flow.. AB - This work concerns a new finite-element formulation for solving hydrody-namic lubrication problems that include partially flooded regions, where the lubricantfilm behavior is governed by free surface flow.. KW - Hydrodynamic Lubrication. KW - Thin Film Flow. KW - Free Surface Flow. KW - Finite- Element. KW - Stabilization. M3 - Conference abstract in proceedings. SP - 166. BT - Proceedings of the 30th Nordic Seminar on Computational Mechanics (NSCM-30). A2 - Høsberg, J. A2 - Pedersen, N.L. T2 - 30th Nordic Seminar on Computational Mechanics (NSCM-30). Y2 - 25 October 2017 through 27 October 2017. ER - ...
The presently acknowledged onset of synaptogenesis in the chick retina from embryonic day 12 (E12) onward stands in contrast with the appearance of spontaneous electrical activity, of presynaptic proteins, or of neurotransmitters during early formation of the inner (E6-E8) and outer (E9) plexiform layers. Therefore, we investigated the chick retina from E6 to E12 at which age first synapses appear by transmission electron microscopy (TEM). The study provides evidence that synaptogenesis in the chick retina begins shortly after the plexiform layers have started to emerge. The first synapses are electrical synapses, which appear on E7, one day after the future inner plexiform layer emerged, and towards the end of E8 in the nascent outer plexiform layer. Conventional chemical synapses appear in both plexiform layers on E8, in the inner plexiform layer (stage 34) only a few hours earlier than in the outer plexiform layer (stage 35). The first synapses are formed close to the apex of the optic fissure and
We report the nanostructure study results, mainly based on plan-view transmission electron microscopy (TEM) on the coalescence process during the overgrowth by metalorganic chemical vapor deposition of GaN nanocolumns grown by molecular beam epitaxy. In cross-section scanning electron microscopy images, one can observe a two-stage coalescence overgrowth process. First, a group of nearby nanocolumns is merged into a thicker column. One of the possible merging processes is the growth of a bridging domain between two columns for their connection. The thicker columns are then developed into horn-shaped structures for the second-stage coalescence. Because different columns may have different crystal orientations, stacking faults can be formed for implementing the coalescence between two domains. Such stacking faults around the boundaries of merged domains represent one of the major kinds of defect after the threading dislocation density is reduced based on the nanocolumn growth technique.. ©2013 ...
In 1981 Taxy et al described first two cases of epithelioid schwannoma. The clinical, gross, and light microscopic features of two epithelioid tumours were not typical of either benign or malignant schwannoma. Electron microscopic features were indicative of benign Schwann cell tumours.. In 1985 Frank et al described electron microscopic features of Schwann cells in epithelioid schwannoma.. It was first reported as cutaneous epithelioid schwannoma by Kindblom et al in 1998.. The tumors are predominantly dermal/subcutaneous in location and involve the lower limb, upper limb, trunk and head/neck. These tumors may cause diagnostic errors due to their increased cellularity and epithelioid morphology. Typical histologic features of classic schwannoma such as Antoni A and B areas, Verocay bodies, and hyalinized vessels are either absent or only present in focal areas. Strong and diffuse S-100 protein expression is seen in both benign and malignant counterparts of epithelioid schwannoma. Type IV ...
TY - JOUR. T1 - An iterative method for obtaining a nonlinear solution for the temperature distribution of a rotating spherical body revolving in an eccentric orbit. AU - Sekiya, Minoru. AU - Shimoda, A. A.. PY - 2014/1/1. Y1 - 2014/1/1. N2 - An iterative method for determining the temperature distribution in a rotating spherical body with an eccentric orbit around a star is developed. The heating term is expanded into the Fourier series with respect to the mean anomaly and the spherical harmonics with respect to the longitude and colatitude of a spherical body. The obtained formula is suitable for the eccentricity less than about 0.7. The remaining procedure to determine the temperature using an iterative method is the same as that described in Sekiya and Shimoda (2013). The method for determining the change rates of orbital elements due to the Yarkovsky effect is also developed. Our method is applicable to any value of the rotation period of a body. The errors of our results are less than 1%. ...
Guinea pigs approximately 8 months old, maintained on a diet of rabbit pellets and carrots, developed abnormalities similar to some of those described for scorbutic guinea pigs (Follis, 1963). Approximately half of the animals developed a fibrovascular proliferation in the thigh muscles which caused up to sixfold increase in the normal volume of the thigh (Meheregon, personal communication). Tissue from the swollen legs grew in culture in Eagle's medium as a confluent, fibroblast-like sheet, whereas normal-sized leg muscles of the same animals did not grow under the same conditions. Increase in cell number occurred for 4 to 6 weeks and then the culture became stationary. This report presents electron microscopic evidence for the presence of virus-like particles, possible developmental forms of a pox virus, in the cells in culture. Cultures were obtained by treatment of the tissues with 0.25% trypsin for 15 min.; the dispersed cells were collected by centrifugation, suspended in tissue culture
Hepatitis A pathogen (HAV) infects African green monkey kidney cells via HAV cellular receptor 1 (havcr-1). to the Fc and hinge portions of human IgG1. D1muc-Fc neutralized 10 occasions more HAV than did D1-Fc. Sedimentation evaluation in sucrose gradients demonstrated that treatment of HAV with 20 to 200 nM D1muc-Fc disrupted a lot of the virions, whereas treatment with 2 nM D1muc-Fc acquired no influence on the sedimentation from the contaminants. Treatment of HAV TAK-875 with 100 nM D1muc-Fc led to low-level deposition of 100- to 125S contaminants. Negative-stain electron microscopy evaluation revealed the fact that 100- to 125S contaminants acquired the features of disrupted virions, such as for example inner staining and diffuse sides. Quantitative PCR evaluation showed the fact that 100- to 125S contaminants included viral RNA. These outcomes indicate that D1 as well as the mucin-like area of havcr-1 must induce conformational adjustments resulting in HAV uncoating. Hepatitis A pathogen ...
The present study was designed to develop a technique to prepare human chromosomes for sequential light and electron microscopic observation and to compare detectability of chromosome aberrations induced by adriamycin and mitomycin C by the two procedures. The technique developed preserved the morphological and structural organization of chromosome while allowing observation of the cells entire chromosome complement. It was rapid and reproducible and chromosomes could be treated and stained for banding. Light microscopic data showed that in cultures of human lymphocytes both drugs induce chromosome aberrations. In comparison with controls both drugs produced significantly more chromosome and chromatid fragments. Electron microscopy revealed greater numbers of chromosome aberrations in both drug groups at higher levels of statistical significance. The differences between chromosome and chromatid fragments observed at the light and electron microscope levels were statistically significant. However, with
Published data are reviewed along with our own data on synaptic plasticity and rearrangements of synaptic organelles in the central nervous system. Contemporary laser scanning and confocal microscopy techniques are discussed, along with the use of serial ultrathin sections for in vivo and in vitro studies of dendritic spines, including those addressing relationships between morphological changes and the efficiency of synaptic transmission, especially in conditions of the long-term potentiation model. Different categories of dendritic spines and postsynaptic densities are analyzed, as are the roles of filopodia in originating spines. The role of serial ultrathin sections for unbiased quantitative stereological analysis and three-dimensional reconstruction is assessed. The authors data on the formation of more than two synapses on single mushroom spines on neurons in hippocampal field CA1 are discussed. Analysis of these data provides evidence for new paradigms in both the organization and ...
Single particle analysis is a group of related computerized image processing techniques used to analyze images from transmission electron microscopy (TEM). These methods were developed to improve and extend the information obtainable from TEM images of particulate samples, typically proteins or other large biological entities such as viruses. Individual images of stained or unstained particles are very noisy, and so hard to interpret. Combining several digitized images of similar particles together gives an image with stronger and more easily interpretable features. An extension of this technique uses single particle methods to build up a three-dimensional reconstruction of the particle. Using cryo-electron microscopy it has become possible to generate reconstructions with sub-nanometer resolution and near-atomic resolution first in the case of highly symmetric viruses, and now in smaller, asymmetric proteins as well. Single particle analysis can be done on both negatively stained and vitreous ...
Fenestrated blood vessels in the rat choroid plexus are permeable to dye-labelled proteins, HRP and ferritin. Most leakage appears to be via fenestrae but some additional escape of marker appears to take place through transient and reversible openings in the junctions between endothelial cells. After they have escaped into the choroidal stroma markers are prevented from entering the CSF by tight junctions between the epithelial cells which cover the choroid plexus, but how they are removed from the extravascular space is not known. Electron microscope study of rats who have been given multiple intravenous injections of ferritin shows that extravascular ferritin is take up both by connective tissue cells in the choroidal stroma and by choroidal epithelial cells. The findings suggest that the ingested protein is subsequently broken down within lysosomal vacuoles in the cytoplasm of these cells. Such intracellular digestion may be the major means of controlling the protein content of the extravascular
The interaction of electrons with solids are briefly reviewed with the emphasis given to elastically and inelastically scattered electrons and the origin of characteristic X-rays, which are used for chemical analysis in electron microscopy. The physical origin of image diffraction and phase contrasts in the transmission electron microscope are discussed. Special attention is paid to the formation of Kikuchi line diffraction patterns. In scanning electron microscopy, Kikuchi lines are used for all orientation imaging techniques (OIM /EBSD) which allow us to determine the orientation of grains and to establish the presence of textures. In the case of transmission electron microscopy, Kikuchi line diffraction patterns are used as crystallographic maps which allow to orient single crystals. It will be explained how simple two beam diffraction contrasts can be obtained and a brief introduction into stereographic 3D methods in scanning and transmission electron microscopy will be given. The students ...
We introduced several membrane-impermeant fluorescent dyes, including Lucifer Yellow, carboxyfluorescein, and fura-2, into the cytoplasmic matrix of J774 cells and thioglycollate-elicited mouse peritoneal macrophages by ATP permeabilization of the plasma membrane and observed the subsequent fate of these dyes. The dyes did not remain within the cytoplasmic matrix; instead they were sequestered within phase-lucent cytoplasmic vacuoles and released into the extracellular medium. We used Lucifer Yellow to study these processes further. In cells incubated at 37 degrees C, 87% of Lucifer Yellow was released from the cells within 30 min after dye loading. The dye that remained within the cells at this time was predominantly within cytoplasmic vacuoles. Lucifer yellow transport was temperature dependent and occurred against a concentration gradient; therefore it appeared to be an energy-requiring process. The fluorescent dyes used in these studies are all organic anions. We therefore examined the ...
The active zone is present in all chemical synapses examined so far and is present in all animal species. The active zones examined so far have at least two features in common, they all have protein dense material that project from the membrane and tethers synaptic vesicles close to the membrane and they have long filamentous projections originating at the membrane and terminating at vesicles slightly farther from the presynaptic membrane. The protein dense projections vary in size and shape depending on the type of synapse examined. One striking example of the dense projection is the ribbon synapse (see below) which contains a ribbon of protein dense material that is surrounded by a halo of synaptic vesicles and extends perpendicular to the presynaptic membrane and can be as long as 500 nm.[3] The glutamate synapse contains smaller pyramid like structures that extend about 50 nm from the membrane.[4] The neuromuscular synapse contains two rows of vesicles with a long proteinaceous band ...
A morphological and ultrastructural study on the prostate of a land snail Nesiohelix samarangae was conducted. The prostate of Nesiohelix samarangae is a tubular gland connected with the large hermaphrodite duct. The lining of the prostate tubules possesses two distinct types of epithelial cells, one secretory and the other non-secretory. The secretory cells contained numerous secretory granules in various sizes and electron density. Most of the secretory granules showed light electron density but some of them showed heavy density. The ciliated cells were non-secreting cells situated only toward the lumen of the tubules and appeared as ordinary epithelial lining cells. The ciliated cells of the epithelium extensively interdigitate with each other and their apical surfaces had numerous cilia and microvilli. The bases of the ciliated cells did not reach the basal region of the secretory cells ...
INTRODUCTION Familial benign hematuria (FBH) (MIM 141200) is an autosomal dominant disease. It is characterized by persistent or recurrent microscopic hematuria, not associated with other abnormalities such as renal failure or deafness1. The diagnosis of this benign disease may be difficult to establish, since it is based on a series of negative findings (absence of proteinuria, renal failure or extrarenal symptoms) and the finding of a non-specific ultrastructural lesion, the thin glomerular basement membrane (GBM), and above all on the results of family investigation demonstrating the absence of progression towards renal failure.. Early stages of Alport Syndrome (AS) can be very similar to FBH, both in its clinical features and the electron microscopic appearance.. AS can be inherited as an X-linked, autosomal dominant or autosomal recessive trait2. X-linked forms account for approximately 85% of AS cases and they are due to mutations in the COL4A5 gene. Autosomal dominant and recessive forms ...
Chair of Ultrapath XVIII - President-elect of the Society for Ultrastructural Pathology. Electron microscopy is a method whose importance for diagnosis and research in Pathology is well established. Unfortunately, the method remains poorly known by the medical profession and is often not part of the standard medical curriculum, precluding its widespread use in diagnosis. Further factors like the use of proper facilities and experience for performing ultrastructural diagnosis have to be addressed but are often underestimated. It is usually acknowledged that many diagnostic applications that in the past relied solely on electron microscopy methods have been replaced by simpler and more accessible methods like immunocytochemistry and molecular biology.. However it must be understood that electron microscopy is still the most reliable way to study macromolecular structure and its relationships within the cell. Electron microscopy is also a catch all method available at the ultrastructural level, ...
Application: To obtain a 3D model for objects that are too thick for single particle electron microscopy (≥ 1 micrometer thick), e.g., small cells, cellular organelles, macromolecules and their complexes, viruses. Method: Samples are vitrified by plunge freezing or by high-pressure freezing, cut into thin slices if necessary, and imaged at various tilt angles in the microscope using the lowest possible electron dose. Imaging under low dose conditions minimizes physical damage of the sample during data acquisition, but leads to extremely low signal-to-noise conditions. Sub-tomogram averaging recovers the signal by an intensive computational procedure, yielding structural resolutions of up to ~1 nm.. ...
To establish the methods of demonstrating early fixation of metal implants to bone, one side of a Cobalt-Chromium (CoCr) based alloy implant surface was seeded with rabbit marrow mesenchymal cells and the other side was left unseeded. The mesenchymal cells were further cultured in the presence of ascorbic acid, β-glycerophosphate and dexamethasone, resulting in the appearance of osteoblasts and bone matrix on the implant surface. Thus, we succeeded in generating tissue-engineered bone on one side of the CoCr implant. The CoCr implants were then implanted in rabbit bone defects. Three weeks after the implantation, evaluations of mechanical test, undecalcified histological section and electron microscope analysis were performed. Histological and electron microscope images of the tissue engineered surface exhibited abundant new bone formation. However, newly formed bone tissue was difficult to detect on the side without cell seeding. In the mechanical test, the mean values of pull-out forces were 77.15 N
Neural circuitry is determined primarily by trillions of synaptic junctions that link cells in the nervous system. Understanding how the structure of the synapse influences its function has been a central goal of cellular neuroscience since synapses were first recognized more than a century ago. Long-term potentiation (LTP), a long lasting enhancement of synaptic efficacy, is a well-characterized cellular correlate of learning and memory that results in dramatic structural remodeling of the synapse. Research has focused heavily on the postsynaptic structural remodeling that occurs to support LTP, but concomitant presynaptic and subcellular remodeling during LTP has been left largely unexplored. To address these questions, three-dimensional reconstructions from serial section electron microscopy of presynaptic boutons, vesicle pools, and dendritic smooth endoplasmic reticulum (SER) in hippocampal area CA1 were created and quantified. The data presented in this dissertation demonstrate that ...
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The CAMCOR Alice C. Tyler Nanofabrication and FIB-SEM Facility provides an array of equipment supporting high resolution electron microscopy, nanofabrication, and material characterization techniques. The facility offers high-resolution scanning electron microscopy and ion-beam microscopy services, as well as electron-beam, and ion beam lithography.. The facility is equipped to support sample types from multiple disciplines, providing sample preparation equipment and services for biological, materials, and geological applications.. ...
Myosin has been identified in a variety of non-muscle cells, and is believed to play a role in maintenance of cell shape, locomotion, cytokinesis, exocytosis and other cellular functions. In this paper we describe the purification of myosin from a pancreatic acinar-cell carcinoma of the rat which forms solid tumours, but retains many differentiated functions. The purified myosin was composed of a 200,000 Da heavy chain and two or three classes of light chains. Electron-microscopic examination of rotary-shadowed preparations revealed that individual molecules had two globular heads and a long tail measuring approx. 149 nm. The myosin was soluble in high-salt buffers and became sedimentable as the ionic strength was lowered. Examination of negative-stained preparations showed that this sedimentable myosin consisted of short, bipolar, thick filaments which had a strong tendency to aggregate in a head-to-head manner. The ATPase activity of the purified myosin was stimulated by EDTA or Ca2+, but not ...
Online quality and compliance store. Buy Refractory materials - Determination of bulk density of granular materials (grain density)
Mitochondria have a central importance: they use electron transport chains and their associated molecular machinery to synthesize ATPs, which provide energy to drive chemical reactions within all eukaryotic cells. Mitochondria are also involved in heme synthesis and lipid metabolism and are highly dynamic, resulting in either large or discrete structures or networks. Characterization of these networks within cells can provide important information about the cells metabolic activity and their overall viability. We have used serial block-face scanning electron microscopy (SBF-SEM) to analyze distributions of mitochondrial network lengths in several types of cells. SBF-SEM can provide high-resolution three-dimensional ultrastructure of large volumes of resin-embedded biological tissue or cells at the nanometer scale, making it possible to image samples extending over ,100 µm in three dimensions, at 10 nm spatial resolution in the plane of the block face (x and y) and 25 nm resolution in the ...
A process for producing flat products from particulate material comprises the steps of forming relatively smooth, castable slurry comprising a suspension of particulate material in an aqueous solution, a film-forming binder material and a dispersion of a particulate synthetic resin in an aqueous solution, depositing a coating of this slurry onto a support surface and, heating the deposited coating to a temperature at which a component of the synthetic resin volatilises. The coating may be removed from the support surface as a flat product either before or after heating to volatilize a component of the synthetic resin.
0046]While various materials have been identified as useful as either high or low threshold percolation concentration materials, as such high and low thresholds are relative terms, certain materials may be usable as either a low or high threshold material, depending upon the threshold percolation wt % concentration of the other conductive particulate material employed. In one embodiment of the invention, the relatively low threshold particulate material and the relatively high threshold particulate material are selected such that their threshold percolation weight percent concentrations are separated by at least 1 wt percent, preferably at least 2 wt percent, more preferably at least 5 wt percent, and most preferably at least 10 wt percent. In a further embodiment, the relatively low threshold particulate material and the relatively high threshold particulate material are selected such that the low threshold particulate material has a percolation threshold wt % concentration of less than 10 ...
In order to reveal the differentiation characteristics of organelles of ciliates under different physiological status, the cellular ultrastructure of Urostyla grandis was studied by transmission electron microscopy. In the resting cells most ciliary shafts, kinetosomes and sub-pellicle microtubules were resorbed, and the endoplasmic reticulum (ER) disappeared with the autophagocytosis taking place within the cytoplasm. As well, the nuclear matrix of the macronucleus was extruded into the cytoplasm, forming pseudopodia-like structures with large quantities of heterochromatin (CH) attached to the inner nuclear membrane. During excystment, membraneous structures developed and gradually increased in number to form the ER. Autophagic vacuoles (AVs) appeared containing mitochondria, paraglycogen particles (PGP), membranous structures, etc. Moreover, the number of nucleoli decreased with the chromatin, condensing in parallel with the process of recombination. Based on these observations, it could be ...
Electron microscopy; Ion microscopy and secondary ion mass spectrometry; Nonimaging optics and solar energy concentration; ...
Transmission electron microscopy: TEM can visualize virion structure, but the imaging procedure is more difficult than SEM, and ... "Electron Microscopy , TEM vs SEM - US". www.thermofisher.com. Retrieved 2021-02-21. Janjic, Aleksandar (2018-11-29). "The Need ... The following methods could offer biosignatures with varying levels of usefulness: Scanning electron microscopy: SEM has ...
Electron microscopy. Histopathology and tissue preservation. Pharmacology and tissue therapy. Immunology. Biochemistry. ...
Wilkinson, A. J.; Hirsch, P. B. (1997). "Electron diffraction based techniques in scanning electron microscopy of bulk ... In the mid-1950s he pioneered the application of transmission electron microscopy (TEM) to metals and developed in detail the ... P. Hirsch, A. Howie, R. Nicholson, D. W. Pashley and M. J. Whelan (1965/1977) Electron microscopy of thin crystals ( ... In 1965, with Howie, Whelan, Pashley and Nicholson, he published the text Electron microscopy of thin crystals. The following ...
... electron microscopy; use of radiochemicals; soil analysis, and plant/animal cell culture. Research was carried out at the Dairy ...
Depending on the desired outcome, both scanning electron microscopy (SEM) and transmission electron microscopy (TEM) can be ... "Electron Microscopy , TEM vs SEM - US". www.thermofisher.com. Retrieved 2022-04-26. Benítez JJ, Guzman-Puyol S, Domínguez E, ... Guignard G (2021-05-18). "Method for ultrastructural fine details of plant cuticles by transmission electron microscopy". ... Atomic force microscopy (AFM) can also be used as a complementary method to provide high-resolution topographic imaging at ...
Electron microscopy. Encyclopedia Britannica. https://www.britannica.com/technology/electron-microscopy Britannica, T. Editors ... SEM-EDS): scanning electron microscopy-energy dispersive spectroscopy is used for directly studying the surfaces of solid ... action of the electron beam stimulates the emission of high-energy backscattered electrons and low-energy secondary electrons ... scientists routinely use scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS), X-ray diffraction (XRD), and ...
The Laboratory Facilities include capabilities in nanopatterning, transmission electron microscopy, nanomaterials synthesis, ... Electron Microscopy; Soft and Biological Materials; Theory and Computation. Scientific highlights within these themes can be ... Access is also offered to the Laser Electron Accelerator Facility (LEAF). The CFN is operated as a national user facility, ...
Scanning Electron Microscopy with Energy Dispersive Spectroscopy (SEM-EDS): Uses an electronic beam to scan the surface of a ... n.d.). Scanning electron microscopy. Retrieved April 19, 2014. CAMEO. (2014). Chromatography. Retrieved April 19, 2014. CAMEO ... n.d.). SEM/EDS: Scanning Electron Microscopy with X-ray microanalysis. Archived 2014-06-08 at the Wayback Machine Retrieved ...
Medical Electron Microscopy. 37 (4): 208-15. doi:10.1007/s00795-004-0261-4. PMID 15614445. S2CID 8188662. Kamimoto, M; Rung- ...
... and electron microscopy. Minimal incubator spaces are available at SABS but they are available at the adjacent Huntsman Marine ...
The TIE method is also applied for phase retrieval in transmission electron microscopy. Bostan, E. (2014). "Phase Retrieval by ... is a computational approach to reconstruct the phase of a complex wave in optical and electron microscopy. It describes the ... Curl, C.L. (2004). "Quantitative phase microscopy: a new tool for measurement of cell culture growth and confluency in situ". ... Using Transport-of-Intensity Equation and Differential Interference Contrast Microscopy". IEEE International Conference on ...
Electron Microscopy Group led by Archie Howie Radio Astronomy (led by Martin Ryle and Antony Hewish), with the Cavendish ... "Electron Microscopy Group". Graham-Smith, F. (1986). "Martin Ryle. 27 September 1918-14 October 1984". Biographical Memoirs of ... Several important early physics discoveries were made here, including the discovery of the electron by J.J. Thomson (1897) the ... Notable discoveries to have occurred at the Cavendish Laboratory include the discovery of the electron, neutron, and structure ...
He is also a world leader in the development and application of atomic-resolution electron microscopy and its use for the study ... Zuo, Jian Min; Spence, John C. H. (2017). Advanced transmission electron microscopy : imaging and diffraction in nanoscience. ... Spence, John (2013). High-resolution electron microscopy. Oxford: Oxford University Press. ISBN 978-0-19-966863-2. "Regent's ... High-resolution electron microscopy (4th ed.). Oxford. ISBN 978-0-19-150840-0. OCLC 862086211. ...
Long GW, Nobel J, Murphy FA, Herrmann KL, Lourie B (September 1970). "Experience with electron microscopy in the differential ... 33-37 Kiselev NA, Sherman MB, Tsuprun VL (1990). "Negative staining of proteins". Electron Microscopy Reviews. 3 (1): 43-72. ... These are solutions of salts of heavy metals, such as tungsten, that scatter the electrons from regions covered with the stain ... Kuznetsov YG, Malkin AJ, Lucas RW, Plomp M, McPherson A (September 2001). "Imaging of viruses by atomic force microscopy". The ...
Medical Electron Microscopy. 36 (2): 94-7. doi:10.1007/s00795-002-0206-8. PMID 12825122. S2CID 1543924. Wu Z, Hoover DM, Yang D ...
"Hot Electron". SCALE Lab. 10 June 2014. Retrieved 26 May 2022. "Scanning Probe Microscopy". SCALE Lab. 10 June 2014. Retrieved ... "빛이 전기로 활발히 전환되는 '핫스팟' 찾았다-나노미터 수준에서 핫전자(hot electron) 거동 관찰…고효율 에너지 소자 응용 기대". Naver (in Korean). 29 January 2019. Retrieved 2 ... Park, Jeong Young; Baker, L. Robert; Somorjai, Gábor A. (22 April 2015). "Role of hot electrons and metal-oxide interfaces in ... Atomic force microscopy has permitted the investigation of nanomechanical, structural properties, and charge transport. 2010- ...
Electron microscopy offers a higher resolution image. In an electron microscope, a beam of focused electrons interacts with the ... Experiments on bilayers often require advanced techniques like electron microscopy and atomic force microscopy. When ... This theory was confirmed through the use of electron microscopy in the late 1950s. Although he did not publish the first ... In conjunction with rapid freezing techniques, electron microscopy has also been used to study the mechanisms of inter- and ...
Levi-Setti, R. (1974). "Proton scanning microscopy: feasibility and promise". Scanning Electron Microscopy: 125. W. H. Escovitz ... The same protocol used for preparing samples to transmission electron microscopy can also be used to select a micro area of a ... For a minimal introduction of stress and bending to transmission electron microscopy (TEM) samples (lamellae, thin films, and ... This technique is particularly suitable for in situ electron microscopy sample preparation. The same successive milling steps ...
Applications to electron microscopy". The Journal of Cell Biology. 66 (1): 198-200. doi:10.1083/jcb.66.1.198. PMC 2109515. PMID ...
Journal of Electron Microscopy. 52 (6): 627-39. doi:10.1093/jmicro/52.6.627. PMID 14756251. Listgarten MA. "University of ...
Tonomura, A. (1998). "Prof Dr Gottfried MOLLENSTEDT (1913-1997)". Journal of Electron Microscopy. 47 (5): 363-364. doi:10.1093/ ... This prism made Möllenstedt a pioneer of electron interferometry. Around 1960 he developed electron and ion beam lithography, ... In 1950, in Mosbach, he found that an electron beam can be split by a thin tungsten wire to create a double image. From this ... 1999). Introduction to Electron Holography. Springer. p. ix. ISBN 978-1-4615-4817-1. Möllenstedt, G. (1941). "Messungen an den ...
Slayter, Elizabeth (1992). Light and Electron Microscopy. Cambridge University Press. ISBN 9780521339483. Hill, Levi L. (1856 ...
"Gap Junctions of Lens Fiber Cells in Freeze-Fracture Replicas". Journal of Electron Microscopy. 1976. doi:10.1093/ ...
Articles to be expanded from March 2016, All articles to be expanded, Articles using small message boxes, Electron microscopy, ... ISBN 978-1-4200-4555-0. Peter W. Hawkes (6 November 2013). The Beginnings of Electron Microscopy. Elsevier Science. ISBN 978-1- ... ISBN 978-0-85296-906-9. Peter W. Hawkes (6 November 2013). The Beginnings of Electron Microscopy. Elsevier Science. p. 369. ... ISBN 978-1-4832-8465-1. Saul Wischnitzer (22 October 2013). Introduction to Electron Microscopy. Elsevier Science. pp. 91-92. ...
Journal of Electron Microscopy. Japan Society Microscopy. 12 (1): 72-73. doi:10.1093/oxfordjournals.jmicro.a049375. Archived ... Stäubli, W. (1963). "A new embedding technique for electron microscopy, combining a water-soluble epoxy resin (Durcupan) with ... It is commonly used for embedding electron microscope samples in plastic so they may be sectioned (sliced thin) with a ...
Electron microscopy of proteins. James R. Harris. London: Academic Press, a subsidiary of Harcourt Brace Jovanovich. ISBN 0-12- ... Journal of Electron Microscopy. 45 (3): 242-246. doi:10.1093/oxfordjournals.jmicro.a023440. ISSN 0022-0744. Takizawa, T.; Saito ... Journal of Electron Microscopy. 46 (1): 85-91. doi:10.1093/oxfordjournals.jmicro.a023494. ISSN 0022-0744. Chayen, J. (1980). ... cryo-electron microscopy, X-ray microanalysis by energy-dispersive X-ray spectroscopy, immunohistochemistry and cytochemistry, ...
1 December 2012). "Prokaryote or eukaryote? A unique microorganism from the deep sea". Journal of Electron Microscopy. 61 (6): ... Robert Hooke, a contemporary of Leeuwenhoek, also used microscopy to observe microbial life in the form of the fruiting bodies ...
"Electron Microscopy Data Bank". Electron Microscopy Data Bank. Retrieved 2021-08-05. Esquivel-Rodríguez, J; Xiong, Y; Han, X; ... Cryo-Electron Microscopy Colocalization Benchmark Source Lawson, Catherine L; Baker, Matthew L; Best, Christoph; et al. ( ... The EM Data Bank or Electron Microscopy Data Bank (EMDB) collects 3D EM maps and associated experimental data determined using ... 30 May 2015). "Navigating 3D electron microscopy maps with EM-SURFER". BMC Bioinform. 16 (181): 181. doi:10.1186/s12859-015- ...
The John M. Cowley Center for High-Resolution Electron Microscopy at Arizona State is named in his honor. John Cowley was an ... He received the highest awards of the International Union of Crystallography, the Electron Microscopy Society of America and ... He made pioneering contributions in the fields of electron microscopy, diffraction and crystallography, all of which brought ... Journal of Electron Microscopy. 54 (3): 151-62. doi:10.1093/jmicro/dfi038. PMID 16123069. Sumio Iijima (2005) In Memory of the ...
Cryo-electron microscopy reveals native polymeric cell wall structure in Bacillus subtilis 168 and the existence of a ... bacteria and fine structures of Enterococcus gallinarum and Streptococcus gordonii septa revealed by cryo-electron microscopy ...
Electron microscopy. *Immunofluorescence. *Fluorescence in situ hybridization. Clinical pathology. *Clinical chemistry. * ...
"Entry and release of poliovirus as observed by electron microscopy of cultured cells". J. Virol. 4 (4): 505-13. 1 October 1969 ...
The conditions for this localization are that there is a high enough density of scatterers in the metal (other electrons, spins ... time-resolved microscopy/spectroscopy, sensing, friend-foe identification, etc. Furthermore, random laser is naturally endowed ... Anderson localization is a well-known phenomenon that occurs when electrons become trapped in a disordered metallic structure, ... The analogy between photons and electrons has encouraged the vision that photons diffusing through a scattering medium could be ...
Randy O. Wayne (16 December 2013). Light and Video Microscopy. Academic Press. ISBN 978-0-12-411536-1. .. ... The effect of free electrons in the ionosphere, in conjunction with the earth's magnetic field, causes Faraday rotation, a sort ... This is the same mechanism which can rotate the axis of linear polarization by electrons in interstellar space as mentioned ...
Using transmission electron microscopy,the three viruses were shown to be similar in shape and have club-like spikes.[32] A ... and refers to how virions look under an electron microscopy (E.M.).[9] They have a fringe of large, bulbous surface projections ... the novel cold virus OC43 had distinctive club-like spikes when observed with the electron microscope.[34][35] ... "The chronicles of coronaviruses: the electron microscope, the doughnut, and the spike". Science Vision. 20 (2): 78-92. doi ...
Ryabchikova, Elena I.; Price, Barbara B. (2004). Ebola and Marburg Viruses: A View of Infection Using Electron Microscopy. ...
Blood tests, dark field microscopy of infected fluid[1][2]. Differential diagnosis. Many other diseases[1]. ... Electron micrograph of Treponema pallidum. විශේෂතාව. Infectious disease. රෝග ලක්‍ෂණ. Firm, painless, non-itchy skin ulcer[1]. ...
Modern research and experiments have been done using low-vacuum scanning electron microscopy of uncoated type material to ... Hall, S.R.; Taylor, P.D.; Davis, SA; Mann, S (2002). "Electron diffraction studies of the calcareous skeletons of bryozoans". ... Reynolds, K.T. (2000). "Taxonomically Important Features on the Surface of Floatoblasts in Plumatella (Bryozoa)". Microscopy ...
Scanning electron and light microscopy of a new cell death induced by sodium ascorbate: Menadione treatment". Scanning. 25 (3 ... Microscopy and Microanalysis. 9(4): 311 -329.. *J. Gilloteaux, J.M. Jamison, D. Arnold, J.L. Summers: 2003. LM and SEM aspects ...
a b Kuipers, J., Giepmans, B.N.G. Neodymium as an alternative contrast for uranium in electron microscopy. Histochem Cell Biol ... Uranyl acetate has been the standard contrasting agent in transmission electron microscopy (TEM) for decades.[80][81] However, ... neodymium usually only uses three electrons as valence electrons, as afterwards the remaining 4f electrons are strongly bound: ... Watson ML (1958b) Staining of tissue sections for electron microscopy with heavy metals. J Cell Biol 4:475-478 ...
Her research has applied microscopy, and in particular environmental scanning electron microscopy to the study of both ... Donald, Athene (1977). Electron microscopy of grain boundary embrittled systems (PhD thesis). University of Cambridge. OCLC ... She pioneered studies of thermotropic liquid crystalline polymers via transmission electron microscopy, revealing the ubiquity ... followed by a PhD in 1977 for research on electron microscopy of grain boundary embrittled systems.[14] ...
Glass knives are used to slice sections for light microscopy and to slice very thin sections for electron microscopy. ... teeth and tough plant matter for both light microscopy and for electron microscopy. Gem-quality diamond knives are also used ... and serial block-face scanning electron microscopy (SBFSEM), and are sometimes also important for light-optical microscopy.[14] ... Electron Microscopy Technique: after embedding tissues in epoxy resin, a microtome equipped with a glass or gem grade diamond ...
Uranyl acetate and uranyl formate are used as electron-dense "stains" in transmission electron microscopy, to increase the ... A uranium atom has 92 protons and 92 electrons, of which 6 are valence electrons. Uranium is weakly radioactive because all ... A team led by Enrico Fermi in 1934 observed that bombarding uranium with neutrons produces the emission of beta rays (electrons ...
However, this model could not explain the observed strength of grain boundaries and, after the invention of electron microscopy ...
... which uses the scintillations provoked by electron-positron annihilation phenomena. ...
... or by electron microscopy. PCR has become available to diagnose CPV2, and can be used later in the disease when potentially ...
Therefore, the best way to diagnose Camelpox is via Transmission Electron Microscopy evaluation of skin samples from infected ...
It is occasionally used as a 0.5% or 1% aqueous negative stain in transmission electron microscopy (TEM) because it shows a ... Electron microscopy stains, Uranyl compounds, Nuclear materials, Formates, All stub articles, Inorganic compound stubs). ... is a salt that exists as a fine yellow free-flowing powder occasionally used in transmission electron microscopy. ... Electron microscope 2SPI.com Archived 2015-07-03 at the Wayback Machine, compound information, retrieved May 3, 2011. v t e ( ...
A description of human papillomavirus (HPV) by electron microscopy was given in 1949, and HPV-DNA was identified in 1963. It ...
The virus particles demonstrate an amorphous surface structure when visualized using electron microscopy. Noroviruses contain a ... electron microscopy on stored human stool samples identified a virus, which was given the name "Norwalk virus". Numerous ...
... brief review and role of scanning electron microscopy in diagnosis". Ultrastructural Pathology. 25 (2): 99-103. doi:10.1080/ ...
... or electron microscopy. Two major types of sections in gross processing are perpendicular and en face sections: Perpendicular ...
Unwin N (2015), "Experiments in electron microscopy: from metals to nerves" Physica Scripta 90:048002 Zuber B, Unwin N (2013 ... "Three-dimensional model of purple membrane obtained by electron microscopy" Nature 257:28-32 Unwin P N T, Henderson R (1975), " ... "Three-dimensional model of membrane-bound ribosomes obtained by electron microscopy" Nature 269:118-122 Henderson R, Unwin P N ... "Phase contrast and interference microscopy with the electron microscope" Phil. Trans. Roy. Soc. Lond. B261:95-104 Unwin was ...
... triple immunogold labeling electron microscopy". Microscopy Research and Technique. 55 (3): 154-63. doi:10.1002/jemt.1166. PMID ...
... electron microscopy and molecular dynamics simulations. These techniques have allowed the discovery and detailed analysis of ... This proton motive force then drives ATP synthesis The electrons needed to drive this electron transport chain come from light- ... The electrons then flow through photosystem I and can then be used to reduce the coenzyme NADP+. This coenzyme can enter the ... The electrons then flow to the cytochrome b6f complex, which uses their energy to pump protons across the thylakoid membrane in ...
When he was around fifteen, he saw a documentary about Louis Pasteur that made him interested in microscopy. Within a few years ... In 1969 he began using a scanning electron microscope on a Life assignment to depict the body's functions. He is generally ...
employed cryo-electron microscopy to determine that, in vivo, only a pentamer or smaller polymer could spatially fit in the ... but these studies had a solely genetic basis rather than a microscopy based approach. In the year 2000, a study by Simpson et ...
... electron microprobe and a micro-SIMS for Secondary Ion Mass Spectrometry, and formed a consortium with other local scientists ... for him and his students to have access to Magic-angle-Spinning Nuclear Magnetic Resonance, Atomic Force Microscopy and X-ray ... operating the electron microprobe and lecturing for other faculty members when they went on sabbatical leave. After seven years ... Luciano Ungaretti and Giuseppe Rossi in Pavia using large-scale crystal-structure refinement and electron-microprobe analysis ...
Electron microscopy can be used to observe the rabies virus in detail. Photos show examples of Rhabdovirus. ... The ultrastructure of viruses can be examined by electron microscopy. Using this method, the structural components of viruses ... Negatively stained Rhabdovirus as seen through an electron microscope. Notice the bullet shape of the virus (A). See the "bee ... When viewed with an electron microscope Rhabdoviruses are seen as bullet-shaped particles. ...
The Electron Microscopy Center (abbr.: EMC) is a scientific user facility at Argonne National Laboratory. The EMC works to ... solve materials problems using their unique capabilities for electron beam characterization. v t e v t e (Articles lacking ...
Find the latest Electron Microscopy news from WIRED. See related science and technology articles, photos, slideshows and videos ...
The scanning electron microscope (SEM) is becoming increasingly recognized as an important instrument for the study of portland ... The scanning electron microscope (SEM) is becoming increasingly recognized as an important instrument for the study of portland ...
Electron Microscopy of Biological Macromolecules. Leerdoelen. At the end of the course, the student is able to:. 1. Describe ... Recent breakthroughs in cryo-electron microscopy (cryo-EM) have revolutionize the field entirely making it often the method of ... This 3-week course is an introduction to electron microscopy (EM), ranging from a general theoretical and practical ... students will conduct image processing of a single particle cryo-electron microscopy data set to determine a high-resolution ...
... Dr. Thomas Heimerl. Cell Biology & Electron Microscopy, AG Prof. Uwe-G. Maier. Karl-von-Frisch-Str. 14, ... Sample preparation for scanning electron microscopy (SEM),. 3-D reconstruction via electron tomography,. Post-processing of ... The core facility for Electron Microscopy is integrated as part of the Laboratory for Cell Biology of Prof. Dr. Uwe-G. Maier. ... best fitting method for the specific research interest and finally investigate the samples in a 200 kV Transmission Electron ...
... bright-field scanning transmission electron microscopy approach. This method allows direct, real-time, high-resolution ... Here, the authors use in situelectron microscopy to identify kinetically-driven phase evolution in magnetite single ... Figure 2: Phase evolution probed by in situ electron diffraction.. (a) Electron diffraction intensity profile (the colour map ... Visualizing non-equilibrium lithiation of spinel oxide via in situ transmission electron microscopy. *Kai He ORCID: orcid.org/ ...
ASPEX is a company that builds tabletop Scanning Electron Microscopes (tabletop SEMs). To promote their product, they are ... ASPEX is a company that builds tabletop Scanning Electron Microscopes (tabletop SEMs). To promote their product, they are ... The SEM can also use x-ray fluorescence (a byproduct of electron bombardment) from the samples for elemental composition ... sometime this spring they are going to drop off a demo unit for a couple of weeks and we are going to have a scanning electron ...
EMS is committed to providing the highest quality microscopy products. Browse by application, product type, and more! ... Electron Microscopy Sciences also offers a comprehensive range of lab supplies and equipment for all fields of microscopy, ... Workshops, One-on-One Training, and Equipment Demos - ranging from a general introduction to the field of electron microscopy ... Citifluor, A Division of Electron Microscopy Sciences, has developed a range of antifadent mounting media which greatly reduce ...
... has extended its leadership in cryo-electron microscopy (cryo-EM) with the introduction of two new instruments: the Thermo ... Scientific Krios G3i and the Thermo Scientific Glacios cryo-transmission electron microscopes (cryo-TEMs). ... Tags: Automation, Drug Discovery, Drugs, Electron, Electron Microscopy, G-Protein, Imaging, Microscopy, Particle Analysis, ... Cryo-electron microscopy advances by Thermo Fisher Scientific drives structural biology research. *Download PDF Copy ...
Electron Microscopy Booking Information QReserve Booking Website How To Book. The department houses both a transmission (TEM) ... and scanning electron microscope (SEM). Using electrons with much smaller wavelengths than visible light, electron microscopes ... Scanning Electron Microscope (SEM). In SEM a beam of electrons (~1-30 kV in energy) is scanned across the surface of the sample ... in scanning transmission electron microscopy (STEM) mode for very high-resolution work. The addition of an energy dispersive X- ...
Electron Microscopy. Course. Course Title. Class. Lab/ Shop. Clinical/ Co-op. Credit. ... This course provides experience characterizing and mapping the surfaces of materials on the nanoscopic scale with electron ... Topics include qualitative and quantitative mapping of surface properties with scanning electron microscopes (SEM) and ... transmission electron microscopes (TEM). Upon completion, students should be able to complete independent laboratory projects ...
GRC presents Liquid Phase Electron Microscopy (GRS), a conference on . ... Liquid Phase Electron Microscopy (GRS) Observing New Pathways of Material Formation. October 8 - 9 Four Points Sheraton / ...
The most common by far are transmission electron microscopy (TEM) and scanning electron microscopy (SEM). ... Electron microscopy (EM) is a whole different animal. EM uses electrons rather than visible light to illuminate samples. ... Transmission Electron Microscope HT7700 / Hitachi / www.hitachi-hta.com Drawbacks of optical microscopy include short depth of ... As the original form of EM, TEM is analogous to optical microscopy. It uses an electron beam to convey an entire image, or part ...
Three Dimensional Electron Microscopy/Particle Picking. From Wikibooks, open books for an open world ... w:Particle picking takes the image obtained from the electron microscopy and its goal it to obtain individual particles from ... "DoG Picker and TiltPicker: Software Tools to Facilitate Particle Selection in Single Particle Electron Microscopy." Journal of ... Retrieved from "https://en.wikibooks.org/w/index.php?title=Three_Dimensional_Electron_Microscopy/Particle_Picking&oldid=3818885 ...
... electron microscope has been a valuable tool in the development of scientific theory and it contributed greatly to biology ... Keyword: Electron microscope (EM), Transmission electron microscopy (TEM), 3D electron diffraction tomography technique etc. ... Electron microscope, Transmission electron microscopy, 3D electron diffraction to-mography technique etc. ... Transmission electron microscopy (TEM) and its facility for electron diffraction has long been a key technique in materials ...
The Laboratory of Electron Microscopy (LEM) has a great deal of experience in the development and use of instrumentation as ... well as in electron microscopy methodology; at present it offers several unique ... Electron microscopy is a globally used technique for the examination of matter with an extremely broad scope of applications in ... Electron microscopy. Electron microscopy is a globally used technique for the examination of matter with an extremely broad ...
Demonstration of a 2 × 2 programmable phase plate for electrons Author(s): Jo Verbeeck, Armand Béché, Knut Müller-Caspary, ...
Nokia sites use cookies to improve and personalize your experience and to display advertisements. The sites may also include cookies from third parties. By using this site, you consent to the use of cookies. Learn more ...
... for materials science Video directory includes links to all of video lecture pages for Transmission electron microscopy for ... Course: Transmission electron microscopy for materials science. Click here to go back.. *Welcome from the teacher ...
Ever since the development of the first optical microscope, the reliance of scientists across disciplines on microscopy has ... In electron microscopy on the other hand, structures can be comprehensively visualized based on their distinct electron density ... Recent developments in optical super resolution microscopy enable high resolution correlative light and electron microscopy, ... Although electron microscopy is a powerful tool, it does not implicitly provide information on the location and activity of ...
Title:Morphomics via Next-generation Electron Microscopy. Authors:Raku Son, Kenji Yamazawa, Akiko Oguchi, Mitsuo Suga, Masaru ... When attempting to observe these ultra-structures, the use of electron microscopy (EM) has become indispensable. However, ...
Electron Microscopy Group Seminars , Strain Measurement in Electronic Devices by Transmission Electron Microscopy ... Strain Measurement in Electronic Devices by Transmission Electron Microscopy. Add to your list(s) Download to your calendar ... Aberration corrected high resolution transmission electron microscopy (HRTEM) coupled with Geometric Phase Analysis (GPA) ... To overcome this, we have developed a new technique called dark-field holography based on off-axis electron holography and dark ...
Personer med emneord «Electron microscopy» Navn. Telefon. E-post. Emneord. Shreyas, Rao Head Engineer +47 22851448 [email protected] ...
A new software package allows for dense electron microscopy reconstructions of neuronal networks in the fruit fly brain, and ... Transmission electron microscopy Is the Subject Area "Transmission electron microscopy" applicable to this article? Yes. No. ...
New Development in Electron Microscopy Grid Vitrification. CryoGenium addresses several common issues that are found in a ... Tags: cryo-EMelectron microscopyMaterials ScienceProduct Resource: Technology NewsTechnology News ... Linkam previewed its new cryo plunger-CryoGenium-an exciting new development in electron microscopy (EM) grid vitrification. ... correlative light and electron microscopy (CLEM), and cryo fluorescence provides very low photo-bleaching and high signal to ...
Electron microscopy of the stacked disk aggregate of tobacco mosaic virus protein journal, August 1974 * Unwin, P. N. T.; Klug ... in hydrated and dry states were characterized by cryogenic transmission electron microscopy (cryo-TEM) and cryogenic electron ... Cryo transmission electron microscopy of liposomes and related structures journal, November 2000 * Almgren, Mats; Edwards, ... Cryo-electron microscopy of artificial biological membranes journal, November 1985 * Lepault, Jean; Pattus, Franc; Martin, ...
However, the resulting two-dimensional images are exceptionally noisy since the electron dose must be kept low to avoid damage ... and extract the inherent molecular flexibility and motions from the cryo-electron micrographs. Finally, I will describe some ...
... by vapor deposition and subsequent annealing of amorphous thin films of germanium was studied by high resolution electron ... microscopy (HREM). The HREM images revealed a strongly varied multiply twinned structure. In some regions of adjacent twins ... Article Characterization of interface structures in nanocrystalline germanium by means of high-resolution electron microscopy ... Characterization of interface structures in nanocrystalline germanium by means of high-resolution electron microscopy and ...
  • When viewed with an electron microscope Rhabdoviruses are seen as bullet-shaped particles. (cdc.gov)
  • Negatively stained Rhabdovirus as seen through an electron microscope. (cdc.gov)
  • The scanning electron microscope (SEM) is becoming increasingly recognized as an important instrument for the study of portland cement clinker, cement, and concrete. (astm.org)
  • The focus is on learning how to operate a transmission electron microscope, including EM sample preparation (2-weeks). (rug.nl)
  • Therefore, it should be possible to find the best fitting method for the specific research interest and finally investigate the samples in a 200 kV Transmission Electron Microscope from JEOL (JEM-2100). (uni-marburg.de)
  • Rendering of ASPEX's tabletop scanning electron microscope. (diybio.org)
  • So sometime this spring they are going to drop off a demo unit for a couple of weeks and we are going to have a scanning electron microscope bonanza . (diybio.org)
  • Since its invention, electron microscope has been a valuable tool in the development of scientific theory and it contributed greatly to biology, medicine and material sciences. (researchgate.net)
  • Transmission Electron Microscope (TEM) combined with precession 3D electron diffraction tomography technique has produced very promising results in the field of crystal structure determination and has the great advantage of requiring very small single crystals (from 25-500 nm) and very small quantity of material. (researchgate.net)
  • The department houses both a transmission (TEM) and scanning electron microscope (SEM). (queensu.ca)
  • Using electrons with much smaller wavelengths than visible light, electron microscopes are capable of resolving smaller features than light microscopes, in the nanometer to angstrom ranges depending on the configuration of the electron microscope. (queensu.ca)
  • Transmission Electron Microscope HT7700 / Hitachi / www.hitachi-hta.com Drawbacks of optical microscopy include short depth of focus and magnification limited to a few thousand times. (labmanager.com)
  • Benchtop Scanning Electron Microscope NeoScope II / JEOL / www.jeolusa.com EM comes in at least five varieties, each with subclassifications. (labmanager.com)
  • The ultimate spatial resolution is achievable by means of the cold field emission source of electrons available in the JEOL JSM 6700F scanning electron microscope with verified resolution of 1 nm at the electron energy of 15 keV. (isibrno.cz)
  • This TLP serves as an introduction to the basic concepts and structure of the transmission electron microscope. (doitpoms.ac.uk)
  • Ever since the development of the first optical microscope, the reliance of scientists across disciplines on microscopy has increased. (rsc.org)
  • The development of the first electron microscope and with it the access to information at the nanoscale has prompted numerous disruptive discoveries. (rsc.org)
  • In 1931, Ernst Ruska invented the transmission electron microscope (TEM). (chemistryviews.org)
  • Instead of refraction of light beams as in a light microscope, electron beams are deflected. (chemistryviews.org)
  • Recent advances in the four-dimensional ultrafast transmission electron microscope (4D-UTEM) with combined spatial and temporal resolutions have made it possible to directly visualize structural dynamics of materials at the atomic level. (nature.com)
  • Crystal lattice plane spacings are a good test of electron microscope stability, and provide an internal reference standard for magnification calibration. (tedpella.com)
  • But an electron microscope can resolve membranes, can resolve small things, and so we said, well, with the electron microscope you could trace the path quite. (webofstories.com)
  • Now, the electron microscope is extremely powerful, but it has a very tiny window, you can only look at a small piece of it at a time. (webofstories.com)
  • That meant for one to do this on a whole organism, we would have to get something that was very, very small in order to fit it into the window of the electron microscope. (webofstories.com)
  • And that of course was the dominant condition saying we must go to micro-metazoa, because they were small and they did fit into the window of an electron microscope. (webofstories.com)
  • The Thermo Scientific Phenom ParticleX Desktop SEM (Scanning Electron Microscope) is a multi-purpose desktop SEM designed for multiple applications at the microscale: additive manufacturing, technical cleanliness, steel manufacturing QC, batteries and energy storage, and gunshot residue analysis. (thermofisher.com)
  • It is a good time to be in the market for a scanning electron microscope (SEM): new products and new accessories are launched every year and there are many levels of performance to choose from. (thermofisher.com)
  • The Thermo Scientific Phenom Pro(X) G6 Desktop SEM is a robust, versatile, and effortless desktop scanning electron microscope designed to expand the capabilities of research facilities. (thermofisher.com)
  • Hence, researchers developed a new approach that interprets the raw data from the electron microscope automatically. (azorobotics.com)
  • JEM-2200FS/CR transmission electron microscope (JEOL, Japan), equipped equipped with a K2 direct electron detector device (GATAN, UK), equipped with a 200-kV field emission gun (FEG) and an omega in-column energy filter. (cicbiogune.es)
  • JEM-1230 transmission electron microscope (JEOL, Japan), equipped with an UltraScan 4000 SP (4008×4008 pixels) cooled slow-scan CCD camera (GATAN, UK), and equipped with a 120-kV LaB6 thermionic gun. (cicbiogune.es)
  • Gatan products, which are fully compatible with nearly all electron microscope models, cover the entire range of the research process-from specimen preparation and manipulation to imaging and analysis. (gatan.com)
  • Limiting exposure to the electron beam is particularly important for biological samples that are primarily composed of a few relatively electron-sensitive elements and offer little intrinsic contrast in an electron microscope. (drugdiscoverytrends.com)
  • To better understand the influence of microscale geochemical and microstructural relationships on the bulk petrophysical properties of unconventional shale systems, core samples from four producing North American formations were cross-sectioned with an argon ion polisher and imaged with a field emission scanning electron microscope (FE-SEM) using a variety of complementary detectors. (jeolusa.com)
  • We report an approach - named chemTEM - to follow chemical transformations at the single-molecule level with the electron beam of a transmission electron microscope (TEM) applied as both a tuneable source of energy and a sub-Angstrom imaging probe. (whiterose.ac.uk)
  • This 'revolution in resolution' is happening largely thanks to new developments of new-generation cameras used for recording the images in the cryo electron microscope which have much increased sensitivity being based on complementary metal oxide semiconductor devices. (inserm.fr)
  • The Philips CM30 is a computer-controlled, intermediate voltage electron microscope. (cnl.ca)
  • For cryo-EM the specimen, typically a purified protein sample, is embedded in vitreous ice on a cryo-EM grid, and is kept at cryogenic temperatures while images are recorded by the electron microscope. (case.edu)
  • This paper presents an overview of the principles and procedures for the quantitative X-ray microanalysis of thin specimens using an analytical electron microscope (AEM) equipped with an energy dispersive X-ray spectrometer (EDS). (elsevier.com)
  • It is equipped with a high-quality 4K CCD camera, and in foreseeable future, we plan to upgrade the microscope with a direct electron detector. (uni-frankfurt.de)
  • Pigments, for example, are comparably dense matter and show up remarkably differentiated under the electron microscope . (ocutox.com)
  • Water was immediately removed by freeze-drying the specimen to prevent any further reaction so that the specimen could be returned to room temperature for examination in a scanning electron microscope (SEM). (syr.edu)
  • This sample was thinned using a focused ion beam in order to image it in a transmission electron microscope which can resolve atoms. (uh.edu)
  • This document is the standard operating procedure (SOP) for the FEI XL-30 scanning electron microscope at UHNF. (uh.edu)
  • Elemental composition of the pigment grains in the layers is usually determined by scanning electron microscope coupled with energy dispersive X-ray microanalysis (SEM-EDS), which has better spatial resolution than the laboratory micro-XRF. (alma-lab.cz)
  • The electron microscope is able to achieve greater magnification and resolution because it uses a high voltage beam of electrons, whose wavelength is very much shorter than that of visible light. (brainkart.com)
  • The resolving power of an electron microscope may be as low as 1-2 nm, enabling us to see viruses, for example, and the internal structure of cells. (brainkart.com)
  • There are two principal types of electron microscope, the transmission electron microscope (TEM) and the scanning electron microscope (SEM). (brainkart.com)
  • The scanning electron microscope was developed in the 1960s and provides vivid, sometimes startling, three-dimensional images of surface structure. (brainkart.com)
  • The JEOL JEM-1400 LaB6 120 kV transmission electron microscope is an easy-to-use, high contrast instrument with excellent imaging (TEM and STEM modes) and analytical (energy dispersive spectroscopy detector) capabilities. (bnl.gov)
  • The E-TEM instrument (FEI Titan 80-300) is an 80 - 300 kV field-emission transmission electron microscope with an objective-lens aberration corrector at spatial resolution of 0.08 nm. (bnl.gov)
  • The FEI Talos F200X is a high-resolution analytical scanning/transmission electron microscope (S/TEM) that is routinely operated at 200 keV. (bnl.gov)
  • This microscope is equipped with an X-FEG electron source module that gives a source brightness four times that of a Schottky FEG emitter. (bnl.gov)
  • It is a 120-200kV scanning transmission and transmission field-emission electron microscope (STEM/TEM) for high-resolution analytical structural characterization. (bnl.gov)
  • The field emission scanning electron microscope (JEOL JSM 7100 FT FE-SEM) at Microtrace provides a new level of resolution and intensity over a conventional SEM . (microtrace.com)
  • Ultimately, this instrument provides the highest microanalysis resolution of any instrument in our laboratory, with the exception of the transmission electron microscope . (microtrace.com)
  • I learnt that there are many process and techniques before we can view our sample in the scanning electron microscope (SEM) and transmission electron microscope (TEM), which broadened my understanding from the AS syllabus, as we just learned about the features of electron microscopes. (in2scienceuk.org)
  • For the SEM we did a Serial BlockFace Scanning Electron Microscope SBF-SEM, which is when the block is cut in the microscope and each time a new cut is made the section that is shown is imaged by back scatter - however, there was a disadvantage with this technique of not being able to look at our previous section. (in2scienceuk.org)
  • In the last part of this work, measurements of the electron beam quality (emittance, spot size, beam profile, electron yield) are conducted using a laser-driven needle emitter in a transmission electron microscope. (uni-goettingen.de)
  • The platform offers electron microscopy measurements by using a Transmission Electron Microscope (TEM) and a Scanning Electron Microscope (SEM), as well as support service with relevant sample preparations techniques such as ion milling. (cicenergigune.com)
  • A key feature of this microscope is the low energy spread of the electron beam (0.7 [email protected] or less) which combined with the SuperTwin objective lens, with a Cs of 1.2 mm, allows the determination of the physical and chemical properties at atomic resolution. (cicenergigune.com)
  • Transmission electron microscope (TEM) uses high energy electron beam to form high resolution images of objects in the nanoscale. (measurlabs.com)
  • CRYO ARM ™ is an electron microscope for observing biomolecules such as proteins at cryo temperature. (jeolbenelux.com)
  • The microscope equips a cold field-emission electron gun, an in-column Omega energy filter, a side-entry liquid nitrogen cooling stage, and an automated specimen exchange system. (jeolbenelux.com)
  • JSM-IT200 is an easy-to-use scanning electron microscope focused on cost performance of high functionalities of JSM-IT500 (higher-end model) of InTouchScope ™ , with significantly higher throughput. (jeolbenelux.com)
  • Due to sensitivity of biological materials to the electron beam of the microscope, only relatively low electron doses can be applied during imaging. (ox.ac.uk)
  • He designed, implemented and manages the newly designed Electron and Advanced Light Microscope facility located within PaM in E208 and E209 in the Sulston Building. (sanger.ac.uk)
  • Sections were cut on a LKB-III ultramicrotome, stained with uranyl acetate and lead citrate, and examined in a Joel X-100 electron microscope. (who.int)
  • Our microscopes are equipped to perform diffraction contrast, higher-resolution phase-contrast microscopy, high-angle annular dark-field microscopy, nanodiffraction, convergent beam electron diffraction, energy-dispersive X-ray spectroscopy, and electron energy-loss spectroscopy. (nrel.gov)
  • ASPEX is a company that builds tabletop Scanning Electron Microscopes ( tabletop SEMs ). (diybio.org)
  • This wide spread use of electron microscopes is based on the fact that they permit the observation and characterization of materials on a nanometer (nm) to micrometer (µm) scale. (researchgate.net)
  • Thermo Fisher Scientific, the world leader in serving science, has extended its leadership in cryo-electron microscopy (cryo-EM) with the introduction of two new instruments: the Thermo Scientific Krios G3i and the Thermo Scientific Glacios cryo-transmission electron microscopes (cryo-TEMs). (news-medical.net)
  • This course provides experience characterizing and mapping the surfaces of materials on the nanoscopic scale with electron microscopes. (forsythtech.edu)
  • Topics include qualitative and quantitative mapping of surface properties with scanning electron microscopes (SEM) and transmission electron microscopes (TEM). (forsythtech.edu)
  • Methodology for the very low energy scanning electron microscopy has been developed at ISI and the corresponding attachments are installed on several microscopes in LEM. (isibrno.cz)
  • Graphitized Carbon Black is a stable and highly reproducible standard and is considered to be a standard resolution test for transmission electron microscopes. (tedpella.com)
  • Since the introduction of electron microscopes in the 1930s, scanning electron microscopy (SEM) has developed into a critical tool within numerous different research fields, spanning everything from materials science to forensics to industrial manufacturing. (thermofisher.com)
  • Scanning electron microscopes in forensic and crime laboratories play many roles, including gunshot residue analysis (GSR) used to determine the chemistry of particles collected from firearm suspects. (thermofisher.com)
  • Our platform offers research institutes and companies access to two cryo-transmission electron microscopes and sample preparation instrumentation, and allows users to check their crystallization experiments on-line. (cicbiogune.es)
  • Gatan, Inc. is the world's leading manufacturer of instrumentation and software used to enhance and extend the operation and performance of electron microscopes. (gatan.com)
  • FEI Company's Falcon Direct Electron Detector for its Titan and Tecnai transmission electron microscopes (TEMs) enables the acquisition of low-noise images of delicate biological samples and other beam-sensitive materials that require low electron dose interactions to prevent radiation damage of the material. (drugdiscoverytrends.com)
  • CWRU cryo-EM facility houses state-of-the-art electron microscopes in a newly renovated space (Titan Krios G3i+GIF+K3 camera, FEI Tecnai F20+Tvips F416&DE20 camera, FEI Tecnai T12+Gatan895 camera). (case.edu)
  • At its three transmission electron microscopes (TEMs), the FCEM currently supports or provides service in both the areas of classical room temperature electron microscopy/tomography (EM/ET) and high resolution cryo-EM/ET. (uni-frankfurt.de)
  • Electron microscopes, which were first developed in the 1930s and 1940s, use ring-shaped electromagnets as 'lenses' to focus the beam of electrons onto the specimen. (brainkart.com)
  • Because the electrons would collide with, and be deflected by, molecules in the air, electron microscopes require a pump to maintain a vacuum in the column of the instru-ment. (brainkart.com)
  • Electron Microscopes can have magnifications of 500000. (josephtartakovsky.com)
  • In order to further advance ultrafast electron microscopes, an improvement of the electron gun is necessary with respect to spatial and temporal beam properties as well as the electron yield. (uni-goettingen.de)
  • Among the different detectors installed in the microscopes for morphological analysis of samples, the presence of the backscattered electron detector giving images with atomic contrast allows to have access to the elemental composition of the samples. (cicenergigune.com)
  • Scanning electron microscopes (SEM) analyses are performed at the ALS laboratory in Danderyd, Sweden. (alsglobal.se)
  • JEOL (Europe) BV offers sales, service, support and applications training for a wide range of JEOL of scanning electron microscopes (SEM), transmission electron microscopes (TEM), NMR spectrometers, ESR spectrometers, mass spectrometers, amino acid analyzers and semiconductor process equipment. (jeolbenelux.com)
  • Our flagship facility provides access to a comprehensive suite of light microscopes, electron microscopes (EM), image processing and analysis software, plus decades of experience from our team of specialists. (plymouth.ac.uk)
  • The EMC works to solve materials problems using their unique capabilities for electron beam characterization. (wikipedia.org)
  • Our Quanta 250 environmental SEM is equipped with detectors for both secondary and backscattered electrons at a range of vacuum pressures, with an EDAX Element EDS detector for elemental X-ray characterization of samples. (queensu.ca)
  • This course will educate on the use of complementary materials characterization techniques through understanding the advantages and limitations of commonly used analytical electron microscopy characterization tools. (ntu.edu.sg)
  • Students will be acquainted with the principles, instrumentation and operation of scanning/transmission electron microscopy (SEM/TEM) for the characterization of inorganic materials. (ntu.edu.sg)
  • Characterization of interface structures in nanocrystalline germanium by means of high-resolution electron microscopy and molecular dynamics simulations" Zeitschrift für Kristallographie - Crystalline Materials , vol. 211, no. 3, 1996, pp. 147-152. (degruyter.com)
  • Here, we investigated the morphology and electronic structure of gold-intercalated epitaxial graphene using a multitechnique approach combining spectroscopic photoemission low-energy electron microscopy (SPELEEM) for chemical and structural characterization at mesoscopic length scale and with transmission electron microscopy (STEM) at the atomic level. (intechopen.com)
  • Moreover, we show how such preparation affords a wider choice of imaging options for both chemical and structural characterization, such as backscatter electron observation at varying beam potentials coupled with x-ray and cathodoluminescence spectroscopic techniques. (jeolusa.com)
  • Equivalent particle sizes determined by the VSSA, X-ray diffraction (XRD) (another method giving access to an equivalent particle size and integrated into our characterization methodology) and scanning electron microscopy (SEM) (reference method) were compared. (univ-lorraine.fr)
  • Transmission electron microscopy was used for the characterization of GaN epitaxial layers grown by molecular-beam epitaxy on two different substrates: sapphire (Al 2 O 3 ) and 6H-SiC. (elsevier.com)
  • Advanced characterization by electron microscopy of nanostructured materials and heterostructures based on strongly correlated systems. (nffa.eu)
  • Proven experience in characterization of physical-chemical properties of materials, as well as good knowledge of typical materials science research topics usually addressed with electron microscopy and complementarity of the latter with other experimental techniques. (nffa.eu)
  • The Electron Microscopy Platform is the center for the microstructural characterization of the materials developed at CIC energiGUNE. (cicenergigune.com)
  • The magnified signal may be observed by electron diffraction, amplitude-contrast imaging such as diffraction contrast, or phase-contrast imaging such as high resolution TEM. (nrel.gov)
  • Transmission electron diffraction patterns help to determine the crystallographic structure of a material. (nrel.gov)
  • This instrument is also equipped with a GIF that enables electron energy loss spectroscopy and elemental mapping for a wide range of elements and energy-filtered electron diffraction and imaging, and an EDS system for compositional analysis and elemental mapping with high spatial resolution. (nrel.gov)
  • Electron diffraction is used to determine the crystallographic structure of materials on a fine scale. (nrel.gov)
  • Transmission electron microscopy (TEM) and electron diffraction, due to their fine spatial resolution, have played a very important role in the microstructural investigations of advanced materials. (nature.com)
  • This course will cover the basic principles and techniques of X-ray diffraction and transmission electron microscopy along with demonstrations of the instrument details and imaging experiments through videos. (freevideolectures.com)
  • A group of advanced participants focused on the analysis of the type of crystal lattice defects by diffraction contrast methods using nanometric electron beam in scanning mode. (ipm.cz)
  • Ultimate spatial resolution and electron diffraction analysis combined with the simultaneous acquisition of spectroscopic data are used to guide the synthesis of new materials as well as to monitor structural developments induced under electrochemical reactions ex-situ. (cicenergigune.com)
  • TEM is used in electron diffraction to determine the crystal structure of solid samples. (measurlabs.com)
  • The high accuracy is achieved by using electron diffraction methods. (measurlabs.com)
  • In SEM a beam of electrons (~1-30 kV in energy) is scanned across the surface of the sample and information collected at each point (pixel) of the sample. (queensu.ca)
  • The sample is illuminated with a parallel beam of electrons under normal incidence and the reflected electrons are projected onto a pixelated detector, where an image is formed. (universiteitleiden.nl)
  • This is achieved by focusing a beam of electrons into a tiny spot and scanning the beam across a sample. (uh.edu)
  • A fine beam of electrons probes back and forth across the surface of the specimen and causes secondary electrons to be given off. (brainkart.com)
  • We needed to use thin slices to ensure the beam of electrons can pass through the sample. (in2scienceuk.org)
  • Thermo Fisher Scientific supplies innovative solutions for electron microscopy and microanalysis. (thermofisher.com)
  • Theory, operation, and application of scanning electron microscopy, scanning probe microscopy, and X-ray microanalysis will be presented. (uiowa.edu)
  • Frozen samples can thus be analysed as solids using Secondary Electron Imaging, Backscattered Electron Imaging or X-ray Microanalysis. (huttonltd.com)
  • Citifluor, A Division of Electron Microscopy Sciences, has developed a range of antifadent mounting media which greatly reduce the fading of the fluorescence of fluorochromes or fluorescent dyes used for labelling biological specimens. (emsdiasum.com)
  • since there exists no comprehensive previous work containing detailed procedures for preparing biological specimens for electron cytochemistry, this series provides detailed descriptions of methodology employed to localize enzymatic activity at the subcellular level. (kuenzigbooks.com)
  • Cryogenic Electron Microscopy (Cryo-EM) is an approach that allows the observation of hydrated biological specimens in their native environment at cryogenic temperatures in TEM. (case.edu)
  • It will be evident from the foregoing description of sample preparation and use of a vacuum that electron microscopy cannot be used to study living specimens. (brainkart.com)
  • During Scanning Electron Microscopy (SEM) investigations, a focussed electron beam scans the surface of specimens. (labnano.pl)
  • Conclusive diagnosis of these viruses requires electron microscopic examination of stool specimens, a laboratory technique that is available only at a few large centers, including CDC. (cdc.gov)
  • In 1972, in the examination of stool specimens, electron microscopy identified the Norwalk agent, the most common viral cause of gastroenteritis outbreaks among adults. (cdc.gov)
  • Liver specimens from each group were fixed for electron microscopy 1 hour in 2% glutaraldehyde with 0.1M cacodylate phosphate (pH 7.6) and were postfixed 15 minutes in 1M OsO 4 . (who.int)
  • FEI 200 kV field emitting gun F20 UT ultra-high resolution STEM fitted with a high-angle angular dark field STEM detector for high-resolution (~0.14 nm) STEM Z-contrast microscopy. (nrel.gov)
  • The Falcon is a direct electron detector with improved quantum efficiency, capturing more information from a given electron dose, and accelerating the rate at which the signal-to-noise ratio improves over the exposure period. (drugdiscoverytrends.com)
  • The unique design of the Falcon detector overcomes the excessive electron beam deterioration that was previously the primary technical challenge in developing a practical direct electron detector. (drugdiscoverytrends.com)
  • The FEI Krios TEM which is equipped with an imaging filter and an electron direct detector is currently mainly dedicated to cryo ET data acquisition and allows state-of-the-art cryo TEM for structural biology projects. (uni-frankfurt.de)
  • The instrument is equipped with an energy filtered secondary electron detector, a short working distance backscatter electron detector which enables working at low voltages, and a through the lens detectors, which can be used individually or in concert to provide numerous types of image contrast. (microtrace.com)
  • As the original form of EM, TEM is analogous to optical microscopy. (labmanager.com)
  • And like optical microscopy, TEM acquires images through conventional digital photography but at pixel densities and gray-scale bandwidths approaching that of direct record film. (labmanager.com)
  • As well as maintaining the integrity of the sample vitrified by liquid nitrogen cooling, the Linkam CMS196V stage enables contamination-free sample handling and transfer for optical microscopy imaging. (labmanager.com)
  • The inherent advantages of scientific charge-coupled device ( CCD ) sensors for digital imaging in optical microscopy have made them ubiquitous in a wide variety of applications. (fsu.edu)
  • EAG uses SEM analysis in cases where optical microscopy cannot provide sufficient image resolution or high enough magnification. (eag.com)
  • Basic principle of transmission electron microscopy is similar to ordinary optical microscopy. (measurlabs.com)
  • Electron microscopic diagnosis is uniquely suited for rapid microscopy fully, it should be quality controlled, applied as a identification of infectious agents. (cdc.gov)
  • Our algorithm is applied to the challenging domain of electron microscopic sections supposedly $45\text{ nm}$ apart, and we show that these images with high certainty belong to the required statistical class, and that the reconstructions are valid. (ku.dk)
  • Unique Scanning Electron Microscopic Features of Hairy Cells in Hairy-Cell Leukemia. (usu.edu)
  • We used an electron microscopic immunocytochemical approach to compare the distribution of DAT protein in the dorsolateral striatum and the deep layers of the prelimbic PFC. (jneurosci.org)
  • In microscopy in chinese men in a method can be exposed areas from electron microscopic protocol. (josephtartakovsky.com)
  • Electron microscopic lung fiber burden analysis revealed over 8,000,000 asbestos fibers per gram dry lung, 68% of which were tremolite asbestos. (cdc.gov)
  • Comparative scanning electron microscopic studies of tegument in various strains of Schistosoma japonicum / by Tunyarut Koonchornboon. (who.int)
  • In the last week, students will conduct image processing of a single particle cryo-electron microscopy data set to determine a high-resolution protein structure (1 week). (rug.nl)
  • DoG Picker and TiltPicker: Software Tools to Facilitate Particle Selection in Single Particle Electron Microscopy. (wikibooks.org)
  • EMBL Heidelberg hosts a cryo-electron microscopy (cryo-EM) service platform, available for use by external scientists with both single particle and tomography projects. (embl.org)
  • The Cryo-Electron Microscopy Service Platform at EMBL Heidelberg is now offering remote service to all interested users, including the COVID-19-related projects (single-particle analysis and cryo-electron tomography). (embl.org)
  • One of these methods is Single Particle Analysis and another method is Cryo-Electron Tomography (cryo-ET). (cicbiogune.es)
  • For many years the lack of sub 3 Å (angstrom) high-resolution structures solved by single particle cryo-electron microscopy (cryo-EM) created speculation that 3 Å might be the fundamental resolution limit of the technique. (gatan.com)
  • The research article 2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20 S proteasome using cryo electron microscopy , eLife 2015;10.7554/eLife.06380, demonstrates that with the help of the K2 Summit camera, single-particle cryo-EM can compete with x-ray crystallography to determine protein structures for rational drug design at 2.8 Å resolution. (gatan.com)
  • Cryo-EM broadly encompasses three different approaches: electron crystallography, single-particle cryo-EM, and electron cryo tomography. (case.edu)
  • In paper A we propose a fast non-linear reconstruction method for joint phase-retrieval and image reconstruction in cryo electron tomography. (kth.se)
  • Cryo-CLEM is a rapidly emerging technique that combines the advantages of fluorescence light microscopy (FLM) with three dimensional (3D) cryo-electron tomography (cryo-ET) to reveal the ultrastructure of target molecules with specific cellular functions. (labmanager.com)
  • Cryo-Electron Tomography. (cicbiogune.es)
  • We are thrilled to invite you to the first webinar dedicated to integrated cryo fluorescence light microscopy (cryo-FLM) and how it can improve the cryogenic electron tomography (cryo-ET) workflow. (delmic.com)
  • This year's prize went to the development of cryo-electron microscopy, a technique that allows the structures of biomolecules to be revealed where other techniques fail. (compoundchem.com)
  • stockholm - The Nobel Prize for Chemistry has gone to three scientists for the development of cryo electron microscopy. (hotrecentnews.com)
  • In fact, 98 percent of all structures contributed to the Electron Microscopy Data Bank (EMDB) with 4 Ångström or better resolution were determined using Thermo Fisher cryo-TEMs. (news-medical.net)
  • When attempting to observe these ultra-structures, the use of electron microscopy (EM) has become indispensable. (arxiv.org)
  • In electron microscopy on the other hand, structures can be comprehensively visualized based on their distinct electron density and geometry. (rsc.org)
  • Labelling of specific structures directly for electron microscopy using small gold nanoparticles ( i.e. immunogold) has been used extensively. (rsc.org)
  • But, cryo-electron microscopy offers only three-dimensional structures, and it cannot identify proteins to categorize their identity. (azorobotics.com)
  • The vulnerability of biological structures to damage by high energy beam electrons precludes the use of extended exposures that could otherwise be used to improve signal-to-noise. (drugdiscoverytrends.com)
  • It offers access to world-leading scanning transmission electron microscopy (STEM) instrumentation and expertise for the direct imaging of atomic structures and the determination of chemical composition, bonding and vibrational properties, with a focus on single-atom precision and sensitivity. (ukri.org)
  • In transmission electron microscopy (TEM), a high-energy electron beam is used to examine the structures of molecules, down to the level of atomic details. (case.edu)
  • Transmission electron microscopy (TEM) can reveal the ultra structural details at cellular levels, whereas Scanning electron microscopy (SEM) can A show the morphology of minute structures/organisms in three-dimensional state. (org.in)
  • The py4DSTEM project, a suite of tools for processing four dimensional scanning transmission electron microscopy (4D-STEM) data, has continued the development of the EMD file format to add several new data structures, including utilities for reading and saving related collections of images, multidimensional data with labeled slices, lists of electron counts, and other rich lists of positions and quantities. (emdatasets.com)
  • University of Cape Town (UCT) researchers have used cryo-electron microscopy to identify the first complete structures of the human angiotensin-converting enzyme (ACE) (cryo-EM). (azolifesciences.com)
  • Cryogenic transmission electron microscopy (cryo-TEM) is a high-resolution biological imaging method, whereby biological samples, such as purified proteins, macromolecular complexes, viral particles, organelles and cells, are embedded in vitreous ice preserving their native structures. (ox.ac.uk)
  • Difficulties in Differentiating Coronaviruses from Subcellular Structures in Human Tissues by Electron Microscopy. (cdc.gov)
  • NREL investigates the structure and chemistry of materials with particular emphasis on defects and interfaces using transmission electron microscopy (TEM) and scanning transmission electron microscopy (STEM). (nrel.gov)
  • The following table is a condensed listing of equipment, techniques, applications, and properties of instrumentation for Transmission/Scanning Transmission Electron Microscopy. (nrel.gov)
  • Here we explore the lithiation of nanosized magnetite by employing a strain-sensitive, bright-field scanning transmission electron microscopy approach. (nature.com)
  • C-flat™ is the premier holey carbon grid for cryo-transmission electron microscopy. (emsdiasum.com)
  • Transmission Electron Microscopy (TEM) has long been used in materials science as a powerful analytical tool. (researchgate.net)
  • In transmission electron microscopy (TEM), a thin sample, less than 200 nm thick, is bombarded by a highly focused beam of single-energy electrons. (researchgate.net)
  • The most common by far are transmission electron microscopy (TEM) and scanning electron microscopy (SEM). (labmanager.com)
  • Transmission electron microscopy is a very important tool in materials science for investigating the fine-scale structure of materials. (doitpoms.ac.uk)
  • Since this objective lens can also be applied to other existing TEM/STEM imaging and analytical techniques, the researchers think that their achievement should have considerable far-reaching effects across the entire field of transmission electron microscopy. (chemistryviews.org)
  • Aberration corrected high resolution transmission electron microscopy (HRTEM) coupled with Geometric Phase Analysis (GPA) provides sufficient signal/noise to measure the displacement fields accurately. (cam.ac.uk)
  • Is the Subject Area "Transmission electron microscopy" applicable to this article? (plos.org)
  • In the current work transmission electron microscopy (TEM) was used to determine potential time-dependent changes in D. magna midgut epithelium ultrastructure upon exposure to CuO NPs compared to bulk CuO at their 48 h EC(50) levels: 4.0 and 175 mg CuO/L, respectively. (unboundmedicine.com)
  • For this purpose, we use transmission electron microscopy (TEM) to determinate the sub-nanometric structure of different types of samples, and high-throughput crystallization experiments and conventional in-house data collection. (cicbiogune.es)
  • During this webinar Dr. Ai Leen Koh will speak about how in-situ transmission electron microscopy (TEM) concerns the examination of dynamic events in a sample under a controlled, externally applied stimulation. (gatan.com)
  • 4th Summer School of Transmission Electron Microscopy in IPM - Ústav fyziky materiálů AV ČR, v. v. i. (ipm.cz)
  • Within the Program New materials based on metals ceramics and composites, prof. Antonín Dlouhý organized the fourth year of the Summer School of Transmission Electron Microscopy in IPN on 9 - 12 July 2019. (ipm.cz)
  • The present work addresses the validation process of an in-house developed image analysis tool to extract fringe length, tortuosity, and separation from high resolution transmission electron microscopy images of carbonaceous materials. (elsevier.com)
  • In order to validate the algorithm, we compare fringe properties that are extracted from high resolution transmission electron microscopy (HRTEM) images through (1) the in-house developed tool (new algorithm) and (2) a tool that has been validated and published (previous algorithm). (elsevier.com)
  • Boehman, André L. / A comparison of soot nanostructure obtained using two high resolution transmission electron microscopy image analysis algorithms . (elsevier.com)
  • AXON redefines the in situ experience by linking the transmission electron microscopy detectors and in situ systems together with a revolutionary new software platform. (protochips.com)
  • CNL's Transmission Electron Microscopy (TEM) facility is located at the Chalk River Laboratories. (cnl.ca)
  • This paper reports the microstructure of hot-pressed ZrB 2 -SiC ceramics with added B 4 C as characterized by transmission electron microscopy. (elsevier.com)
  • The successful candidate will work at the experimental activities concerning characterisation by scanning and transmission electron microscopy of nanostructured materials, with particular reference to heterostructures based on strongly correlated materials. (nffa.eu)
  • Proven experience in transmission electron microscopy and sample preparation techniques. (nffa.eu)
  • Transmission electron dense probe and methods and electron microscopy in fact that hold the application. (josephtartakovsky.com)
  • Seed coat development of Harpagophytum procumbens (Devil's Claw) and the possible role of the mature seed coat in seed dormancy were studied by light microscopy (LM), transmission electron microscopy (TEM) and environmental scanning electron microscopy (ESEM). (nwu.ac.za)
  • Transmission electron microscopy of ultrathin liver section from a hamster infected with yellow fever virus taken day 6 post-infection showing apoptotic hepatocytes ( arrows ) and accumulation of lipid droplets ( asterisks ) in the cytoplasm of viable hepatocytes. (ajtmh.org)
  • A new approach for characterizing the vesicle membrane morphology based on low-dose cryogenic electron microscopy (cryo-EM) is described. (osti.gov)
  • In 2016, Dr. Ward's lab used a technique called cryogenic electron microscopy to capture the first high-resolution image of this virus' spike protein. (medlineplus.gov)
  • The core facility for Electron Microscopy is integrated as part of the Laboratory for Cell Biology of Prof. Dr. Uwe-G. Maier. (uni-marburg.de)
  • Electron Microscopy Sciences also offers a comprehensive range of lab supplies and equipment for all fields of microscopy, histology and general laboratory research. (emsdiasum.com)
  • The histology laboratory provides services for researchers and students to process their tissues and cells for histopathological examination.We offer paraffin, cryo, and electron microscopy techniques including processing, embedding, sectioning, staining and imaging. (umanitoba.ca)
  • The Laboratory possess scanning electron microscopy for morphological characterisation, chemical composition analysis. (labnano.pl)
  • Electron Microscopy Laboratory EMSL Analytical, Inc. (electronmicroscopylaboratory.com)
  • Retrieved on December 03, 2022 from https://www.news-medical.net/news/20170809/Cryo-electron-microscopy-advances-by-Thermo-Fisher-Scientific-drives-structural-biology-research.aspx. (news-medical.net)
  • In the article, "Automated Detection and Localization of Synaptic Vesicles in Electron Microscopy Images," by Barbara Imbrosci, Dietmar Schmitz, and Marta Orlando, which was published online on January 4, 2022 , the incorrect image was published for Extended Data Figure 3-2 because of a production error. (eneuro.org)
  • This proposal requests funding for the provision of SuperSTEM, the EPSRC National Research Facility for Advanced Electron Microscopy (AdvEM), for 5 years from 14 March 2022, together with additional capital funding for a unique, next-generation instrument with capabilities tailored for the study of quantum materials and phenomena. (ukri.org)
  • To request histology services, electron microscopy or imaging facility services, please contact the histology lab for a consultation. (umanitoba.ca)
  • The Electron Microscopy and Crystallography Platform is an open access facility at the CIC bio GUNE. (cicbiogune.es)
  • The CFN XPEEM/LEEM facility at the Electron Spectro-Microscopy (ESM, 21-ID-2) beamline at the National Synchrotron Light Source II (NSLS-II) has capabilities for full-field imaging at nanometer scale, as well as the micro-spot spectroscopy, including uXPS, uXAS and uARPES. (bnl.gov)
  • The facility also assists users in microscopy-related experimental design protocol modification and the preparation of. (josephtartakovsky.com)
  • Scientists have for the first time photographed an autoantibody coupled to a receptor on the surface of a nerve cell using the Cryo-Electron Microscopy Facility at UT Southwestern. (azolifesciences.com)
  • The SEM-FIB microscopy facility at IMPMC provides Scanning Electron Microscopy (SEM) and Focused Ion Beam (FIB) expertise for all researchers (from academy to industry) needing versatile, high resolution, state-of-the art electron and ion microscopies to characterize their materials at the nanoscale. (respore.fr)
  • Elemental profile that are being clarified or a more than one of bacteria, the definition of the number and techniques used essentially the protocols and electron microscopy methods in the university. (josephtartakovsky.com)
  • During this workshop, the fundamentals of STEM, basic and advanced STEM imaging (HAADF, ABF, iDPC, and 4D STEM) as well as acquisition and analysis of electron energy loss spectroscopy (EELS) and energy-dispersive x-ray spectroscopy (EDX) data. (icmab.es)
  • The EMS web site offers the most up to date information on the microscopy and histology market: the product line has been expanded to include products for biological sciences, materials science and histology. (aurion.nl)
  • On behalf of the Turkish Society for Electron Microscopy (TEMD), we are honored to announce that 24th National Congress of Electron Microscopy (EMK 2019) with International Participation will be held at the Balkan Congress Center, in April 24-26, 2019, Edirne, Turkey.The event will be organized by the host institution, Department of Histology and Embryology, Faculty of Medicine, Trakya University. (kongreadresi.com)
  • While correlative light and electron microscopy techniques have attempted to unify the two modalities, the resolution mismatch between the two data sets poses major challenges. (rsc.org)
  • Recent developments in optical super resolution microscopy enable high resolution correlative light and electron microscopy, however, with considerable constraints due to sample preparation requirements. (rsc.org)
  • Recently, the use of correlative cathodoluminescence electron microscopy (CCLEM) imaging based on luminescent inorganic nanocrystals has been proposed. (rsc.org)
  • In this review, we discuss the opportunities of (volumetric) multi-color single protein labelling based on correlative cathodoluminescence electron microscopy, and its prospective impact on biomedical research in general. (rsc.org)
  • We elaborate on the potential challenges of correlative cathodoluminescence electron microscopy-based bioimaging and benchmark CCLEM against alternative high-resolution correlative imaging techniques. (rsc.org)
  • Diagnostic electron microscopy has two advantages over ance of vesicular lesions or respiratory illness in farm animals enzyme-linked immunosorbent assay and nucleic acid amplifi- may be evidence of an emerging disease, a possible zoonosis, cation tests. (cdc.gov)
  • Applications of diagnostic electron microscopy in clinically or outbreak-associated agents as diverse as Nipah virus (6) and epidemiologically critical situations as well as in bioterrorist gastroenteric agents (7) indicate that identification of an out- events are discussed. (cdc.gov)
  • Diagnostic electron microscopy of tumours / Feroze N. Ghadially. (who.int)
  • TTP Labtech Ltd, a global leader in the design and development of automated instrumentation and consumables for life science applications, has announced the introduction of chameleon, an industry-enabling sample-preparation system for cryo-electron microscopy (cryo-EM). (technologynetworks.com)
  • Through the technique of field emission, the filament is heated to a lower temperature, and it is placed within a strong "field," generating electrons within a much narrower energy range that are easier to focus, especially at lower accelerating voltage. (labmanager.com)
  • The evolution of field emission has brought constant improvement to electron microscopy," comments Vern Robertson, SEM manager at JEOL (Peabody, MA). (labmanager.com)
  • The project is the brainchild of Dr Natasha Stephen , Lecturer in Advanced Analysis (Earth & Planetary Sciences) and Director of the Plymouth Electron Microscopy Centre at the University of Plymouth. (plymouth.ac.uk)
  • The much smaller wavelength of the electron beams allows a magnification that is thousands of times higher. (chemistryviews.org)
  • Scanning electron microscopy is a technique for imaging with up to 800,000X magnification. (uh.edu)
  • Two operation regimes are identified offering high magnification or a large electron count rate. (uni-goettingen.de)
  • Our application specialist Marit Smeets will give an overview of all the steps that are involved in the current sample preparation method, specifically focusing on the cryo fluorescence light microscopy (cryo-FLM). (delmic.com)
  • Persistent measles virus infection of the intestine: confirmation by immunogold electron microscopy. (bmj.com)
  • This study sought to investigate persistent measles virus infection of the intestine: a novel protocol for immunogold electron microscopy was developed using a polyclonal anti-measles nucleoprotein antibody on reprocessed, formalin fixed paraffin wax embedded tissue sections. (bmj.com)
  • A complete product catalog of the entire Diatome collection of Diamond knives, tools, and accessories for Electron and Light microscopy for Biological and Materials Science at room and cryo temperatures. (emsdiasum.com)
  • Optical, or light, microscopy systems are the most familiar. (labmanager.com)
  • EM uses electrons rather than visible light to illuminate samples. (labmanager.com)
  • Because electron wavelengths can be as much as 10,000 times shorter than those of visible light, the resolving power of EM is significantly higher-up to about 10 million times. (labmanager.com)
  • The electrons lose energy as they interact with the sample, resulting in the emission of lower-energy electrons, visible light, or X-rays. (labmanager.com)
  • PMTs record not entire images but flashes of light generated by reflected or transmitted electrons. (labmanager.com)
  • An innovative method of amplifying low-light-level signals above the CCD read noise is employed in electron multiplying CCD technology. (fsu.edu)
  • Finally, I will describe some very recent work where we try to combine experimental and theoretical methods to study ensembles of molecular conformations, and how we would like to use these types of reconstruction methods for wider classes of experimental data, including e.g. neutron scattering and light microscopy. (albanova.se)
  • Antibody binding was detected using both immunoperoxidase and light microscopy on tissue sections, and 10 nm gold conjugated secondary antibody and electron microscopy on ultrathin sections. (bmj.com)
  • Light microscopy (LM) and scanning electron microscopy (SEM) revealed that the recovered parasite belongs to Postorchigenes sp. (entomoljournal.com)
  • By light microscopy, avidin-biotin-peroxidase immunostaining for DAT was visualized in fibers that were densely distributed within the dorsolateral striatum and the superficial layers of the dorsal anterior cingulate cortex. (jneurosci.org)
  • Light and electron microscopy the respirable or near respirable dusts which are. (josephtartakovsky.com)
  • Instead of light TEM uses a transmitted electron beam that penetrates the sample. (measurlabs.com)
  • Because the electrons have smaller wavelength than light, the formed image has a lot higher resolution compared to traditional light microscopy. (measurlabs.com)
  • David's role is to provide imaging support for Pathogens and Microbes and access for all campus staff to electron and advanced light microscopy platforms and services. (sanger.ac.uk)
  • The observed twisting occurs because the CMB light rays experience gravitational lensing as they encounter lumps of matter en route through the Universe to us. (aps.org)
  • This entails single crystal structure determination, transmission and scanning electron microscopy together with a wide range of methods for sample preparation. (mpg.de)
  • After gradually moving away from preparation methods prone to artefacts such as plastic embedding and negative staining for cell sections and single particles, the field of cryo electron microscopy (cryo-EM) is now heading off at unprecedented speed towards high-resolution analysis of biological objects of various sizes. (inserm.fr)
  • The series is designed to "provide a complete understanding of electron cytochemical methods. (kuenzigbooks.com)
  • Electron Microscopy Methods and Protocols 3rd Ed John Kuo Ed Humana Press Totowa NJ 2014799 pages ISBN 97-1627037754 Volume 20 Issue. (josephtartakovsky.com)
  • Crefollowing inflammation on electron microscopy methods and protocols. (josephtartakovsky.com)
  • Environmental tem and electron microscopy methods shown us? (josephtartakovsky.com)
  • This course is designed to train participants in the application of various microscopy methods for their own research needs. (uiowa.edu)
  • Recent breakthroughs in cryo-electron microscopy (cryo-EM) have revolutionize the field entirely making it often the method of choice for high resolution structure determination of proteins, which was acknowledged by the Nobel Prize in Chemistry in 2017. (rug.nl)
  • Below are recordings of the LMB's 2017 Cryo-microscopy course. (cam.ac.uk)
  • Learners will gain an understanding of the importance of various analytical electron microscopy tools individually and complementary to each other in designing and solving real-world industrial problems. (ntu.edu.sg)
  • While recent studies suggest that health- the undirected, "open view" of electron microscopy allows rapid care systems are ill prepared to treat victims and contain the morphologic identification and differential diagnosis of different spread of an infectious agent (5), the performance of physi- agents contained in the specimen. (cdc.gov)
  • Rapid viral diagnosis of Orthopoxviruses by electron microscopy: optional or a must? (josephtartakovsky.com)
  • With STEM , a highly-focused electron probe is raster-scanned across the material, and various types of scattering are collected as a function of position. (nrel.gov)
  • This in turn is making it possible to repeat the reconstructions iteratively, which enables us to handle large biomolecular complexes (for instance ribosomes) as multiple semi-independent bodies, and extract the inherent molecular flexibility and motions from the cryo-electron micrographs. (albanova.se)
  • Scanning electron microscopy is typically used to image inorganic material, but it can also be used to image biological matter with special processing. (uh.edu)
  • Learn various procedures for preparing biological samples for scanning electron microscopy. (uh.edu)
  • Ultrafast electron microscopy is an emerging field of research offering the potential to investigate structural processes on a femtosecond time-scale with nanometer resolution. (uni-goettingen.de)
  • Electron energy losses can be detected using a Gatan image filter (GIF) to map the compositional and electronic properties of materials. (nrel.gov)
  • Electron microscopy under ultrahigh vacuum conditions of 10-8 Pa enables one to study atomically clean surfaces that can be cleaned in-situ with ion beam bombardment. (isibrno.cz)
  • Workshops, One-on-One Training, and Equipment Demos - ranging from a general introduction to the field of electron microscopy to very specific techniques. (emsdiasum.com)
  • Automatic particle selection from electron micrograph using machine learning techniques. (wikibooks.org)
  • see Figure 1), spinning disk confocal in live-cell imaging, flux determinations of calcium or other ions, and time-resolved three-dimensional microscopy (4-D techniques), the electron multiplying CCD offers significant advantages over other sensors designed for low signal levels. (fsu.edu)
  • Understand more about electron microscopy and how various techniques could help your research. (liverpool.ac.uk)
  • Scanning Electron Microscopy is an international journal of Scanning Electron Microscopy, Related Techniques, and Applications. (usu.edu)
  • David has expertise and experience in development and design of ultrastructural preservation techniques for electron microscopy, including fine structure immunocytochemistry, and also 3D confocal imaging of in vitro infection models using intestinal organoids. (sanger.ac.uk)
  • The sample preparation for electron microscopy is of utmost importance. (mpg.de)
  • Excellent sample preparation is the prerequisite for first-class electron microscopy. (leica-microsystems.com)
  • Electron Microscopy Sciences is specialized in the manufacturing, preparation, and distribution of only the highest quality chemicals, supplies, and equipment, for microscopy, and all of its related fields. (aurion.nl)
  • Thermo Fisher Quanta 250 eSEM with Everhart-Thornley, Concentric Backscattered Electron, Large Field, and Gaseous Secondary Electron detectors for variable pressure measurement of both secondary and backscattered electrons. (queensu.ca)
  • HAADF and on-axis BF/DF2/4 solid state detectors for annular collection of scattered electrons in scanning mode. (queensu.ca)
  • Robertson explains that the improvement in resolution did not result from any single development but by incremental enhancements to electron guns, lenses, detectors, X-ray spectrometers, and other components. (labmanager.com)
  • Secondary electron and BSE-TOPO SEM images show that coccolith calcite preservation is moderate or moderate to poor. (uea.ac.uk)
  • The transmitted electrons at high scattering angle can be collected to form high-resolution, chemically sensitive, atomic number (Z-) contrast images. (nrel.gov)
  • However, the resulting two-dimensional images are exceptionally noisy since the electron dose must be kept low to avoid damage, and averaging data over many images poses other problems, in particular due to motions in molecules. (albanova.se)
  • For proteins, the microscopy enables highly elaborate images of the structure to be created at the level of single-atom. (azorobotics.com)
  • The super-resolution K2 Summit camera uses electron counting to record the highest resolution images with the lowest noise. (gatan.com)
  • Scanning Electron Microscopy (SEM) provides high-resolution and high-depth-of-field images of the sample surface and near-surface. (eag.com)
  • To date, most electron microscopy data is stored in either raw data formats (binary, bitmap images, tiff, etc.) or proprietary formats developed by vendors (dm3, emispec, etc. (emdatasets.com)
  • images are built up from low energy secondary electrons which reflect the topography of the sample. (huttonltd.com)
  • Some materials do not last the high energy electron beam. (measurlabs.com)
  • Made in Switzerland exclusively for Electron Microscopy Sciences, these tweezers are hand-selected by our experts for your specific requirements. (emsdiasum.com)
  • Electron microscopy is a globally used technique for the examination of matter with an extremely broad scope of applications in life, materials and even social sciences. (isibrno.cz)
  • Among the sources of vibration, the low frequency vibration is not perceptible during daily activities, and can adversely affect high-performance metrology tools in the fields of semiconductors, display manufacturing, electron microscopy, photonics and life sciences. (elementpi.com)
  • 24th National Congress of Electron Microscopy, as abiannual event, provides synergy for close cooperation, exchange of information and collaborations and brings together all microscopical studies both in life and material sciences. (kongreadresi.com)
  • Environmental scanning electron microscopy (ESEM) enables the study of samples of living substances and materials under elevated pressure of surrounding gas up to 3000 Pa. (isibrno.cz)
  • These results demonstrate that our UTEM can be used for the study of nanoscale energy transport, electron-phonon coupling and lattice relaxation in picosecond timescales. (nature.com)
  • Low Energy Electron Microscopy (LEEM) is a microscopy technique typically used to study surface processes. (universiteitleiden.nl)
  • This study investigates the use of Scanning electron microscopy-energy-dispersive X-ray (SEM-EDX) as a diagnostic tool for the determination of the osseous origin of samples subjected to different temperatures. (tees.ac.uk)
  • Electron Microscopy is an extremely versatile tool which allows the study of both morphology and material composition from virtually all areas of science and technology. (huttonltd.com)