The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES.
Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.
Silicone polymers which consist of silicon atoms substituted with methyl groups and linked by oxygen atoms. They comprise a series of biocompatible materials used as liquids, gels or solids; as film for artificial membranes, gels for implants, and liquids for drug vehicles; and as antifoaming agents.
Microdevices that combine microfluidics technology with electrical and/or mechanical functions for analyzing very small fluid volumes. They consist of microchannels etched into substrates made of silicon, glass, or polymer using processes similar to photolithography. The test fluids in the channels can then interact with different elements such as electrodes, photodetectors, chemical sensors, pumps, and valves.
The design or construction of objects greatly reduced in scale.
Methods of creating machines and devices.
The preparation and analysis of samples on miniaturized devices.
The study of microorganisms such as fungi, bacteria, algae, archaea, and viruses.
Communication, in the sense of cross-fertilization of ideas, involving two or more academic disciplines (such as the disciplines that comprise the cross-disciplinary field of bioethics, including the health and biological sciences, the humanities, and the social sciences and law). Also includes problems in communication stemming from differences in patterns of language usage in different academic or medical disciplines.
Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A plant family of the order Bromeliales, subclass Zingiberidae, class Liliopsida (monocotyledons).
The branch of chemistry dealing with detection (qualitative) and determination (quantitative) of substances. (Grant & Hackh's Chemical Dictionary, 5th ed)
Alicyclic hydrocarbons in which three or more of the carbon atoms in each molecule are united in a ring structure and each of the ring carbon atoms is joined to two hydrogen atoms or alkyl groups. The simplest members are cyclopropane (C3H6), cyclobutane (C4H8), cyclohexane (C6H12), and derivatives of these such as methylcyclohexane (C6H11CH3). (From Sax, et al., Hawley's Condensed Chemical Dictionary, 11th ed)
Inorganic compounds that contain fluorine as an integral part of the molecule.
Methodologies used for the isolation, identification, detection, and quantitation of chemical substances.
A non-crystalline form of silicon oxide that has absorptive properties. It is commonly used as a desiccating agent and as a stationary phase for CHROMATOGRAPHY. The fully hydrated form of silica gel has distinct properties and is referred to as SILICIC ACID.
Unctuous combustible substances that are liquid or easily liquefiable on warming, and are soluble in ether but insoluble in water. Such substances, depending on their origin, are classified as animal, mineral, or vegetable oils. Depending on their behavior on heating, they are volatile or fixed. (Dorland, 28th ed)
Manufacturing technology for making microscopic devices in the micrometer range (typically 1-100 micrometers), such as integrated circuits or MEMS. The process usually involves replication and parallel fabrication of hundreds or millions of identical structures using various thin film deposition techniques and carried out in environmentally-controlled clean rooms.
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
The process of finding chemicals for potential therapeutic use.
Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal.
A multistage process that includes cloning, physical mapping, subcloning, sequencing, and information analysis of an RNA SEQUENCE.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
The pattern of GENE EXPRESSION at the level of genetic transcription in a specific organism or under specific circumstances in specific cells.
A class of devices combining electrical and mechanical components that have at least one of the dimensions in the micrometer range (between 1 micron and 1 millimeter). They include sensors, actuators, microducts, and micropumps.
Using an INTERNET based personal journal which may consist of reflections, comments, and often hyperlinks.
The development and use of techniques to study physical phenomena and construct structures in the nanoscale size range or smaller.

The pressure-dependence of the size of extruded vesicles. (1/884)

Variations in the size of vesicles formed by extrusion through small pores are discussed in terms of a simple model. Our model predicts that the radius should decrease as the square root of the applied pressure, consistent with data for vesicles extruded under various conditions. The model also predicts dependencies on the pore size used and on the lysis tension of the vesicles being extruded that are consistent with our data. The pore size was varied by using track-etched polycarbonate membranes with average pore diameters ranging from 50 to 200 nm. To vary the lysis tension, vesicles made from POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine), mixtures of POPC and cholesterol, and mixtures of POPC and C(16)-ceramide were studied. The lysis tension, as measured by an extrusion-based technique, of POPC:cholesterol vesicles is higher than that of pure POPC vesicles whereas POPC:ceramide vesicles have lower lysis tensions than POPC vesicles.  (+info)

The shape parameter of liposomes and DNA-lipid complexes determined by viscometry utilizing small sample volumes. (2/884)

A minicapillary viscometer utilizing <0.5 ml of sample at a volume fraction of <0.1% is described. The calculated a/b of DPPC/DPPG multilamellar liposome was 1.14 as prolate ellipsoids and a/b of dioleoylpropyltrimethyl ammonium methylsulfate-DNA complex at a charge ratio of 4:1 (+/-) was 3.7 as prolate ellipsoids or 4.9 as oblate ellipsoids. The deviation of shape from perfect sphere is thus expressed quantitatively in more than two significant figures. In these measurement, the necessary amount of DNA is <0.5 mg.  (+info)

Recovery, visualization, and analysis of actin and tubulin polymer flow in live cells: a fluorescent speckle microscopy study. (3/884)

Fluorescent speckle microscopy (FSM) is becoming the technique of choice for analyzing in vivo the dynamics of polymer assemblies, such as the cytoskeleton. The massive amount of data produced by this method calls for computational approaches to recover the quantities of interest; namely, the polymerization and depolymerization activities and the motions undergone by the cytoskeleton over time. Attempts toward this goal have been hampered by the limited signal-to-noise ratio of typical FSM data, by the constant appearance and disappearance of speckles due to polymer turnover, and by the presence of flow singularities characteristic of many cytoskeletal polymer assemblies. To deal with these problems, we present a particle-based method for tracking fluorescent speckles in time-lapse FSM image series, based on ideas from operational research and graph theory. Our software delivers the displacements of thousands of speckles between consecutive frames, taking into account that speckles may appear and disappear. In this article we exploit this information to recover the speckle flow field. First, the software is tested on synthetic data to validate our methods. We then apply it to mapping filamentous actin retrograde flow at the front edge of migrating newt lung epithelial cells. Our results confirm findings from previously published kymograph analyses and manual tracking of such FSM data and illustrate the power of automated tracking for generating complete and quantitative flow measurements. Third, we analyze microtubule poleward flux in mitotic metaphase spindles assembled in Xenopus egg extracts, bringing new insight into the dynamics of microtubule assemblies in this system.  (+info)

Electrokinetic stretching of tethered DNA. (4/884)

During electrophoretic separations of DNA in a sieving medium, DNA molecules stretch from a compact coil into elongated conformations when encountering an obstacle and relax back to a coil upon release from the obstacle. These stretching dynamics are thought to play an important role in the separation mechanism. In this article we describe a silicon microfabricated device to measure the stretching of tethered DNA in electric fields. Upon application of an electric field, electro-osmosis generates bulk fluid flow in the device, and a protocol for eliminating this flow by attaching a polymer brush to all silicon oxide surfaces is shown to be effective. Data on the steady stretching of DNA in constant electric fields is presented. The data corroborate the approximate theory of hydrodynamic equivalence, indicating that DNA is not free-draining in the presence of both electric and nonelectric forces. Finally, these data provide the first quantitative test of a Stigter and Bustamante's detailed theory of electrophoretic stretching of DNA without adjustable parameters. The agreement between theory and experiment is good.  (+info)

Hydrostatic pressurization and depletion of trapped lubricant pool during creep contact of a rippled indenter against a biphasic articular cartilage layer. (5/884)

This study presents an analysis of the contact of a rippled rigid impermeable indenter against a cartilage layer, which represents a first simulation of the contact of rough cartilage surfaces with lubricant entrapment. Cartilage was modeled with the biphasic theory for hydrated soft tissues, to account for fluid flow into or out of the lubricant pool. The findings of this study demonstrate that under contact creep, the trapped lubricant pool gets depleted within a time period on the order of seconds or minutes as a result of lubricant flow into the articular cartilage. Prior to depletion, hydrostatic fluid load support across the contact interface may be enhanced by the presence of the trapped lubricant pool, depending on the initial geometry of the lubricant pool. According to friction models based on the biphasic nature of the tissue, this enhancement in fluid load support produces a smaller minimum friction coefficient than would otherwise be predicted without a lubricant pool. The results of this study support the hypothesis that trapped lubricant decreases the initial friction coefficient following load application, independently of squeeze-film lubrication effects.  (+info)

Millisecond kinetics on a microfluidic chip using nanoliters of reagents. (6/884)

This paper describes a microfluidic chip for performing kinetic measurements with better than millisecond resolution. Rapid kinetic measurements in microfluidic systems are complicated by two problems: mixing is slow and dispersion is large. These problems also complicate biochemical assays performed in microfluidic chips. We have recently shown (Song, H.; Tice, J. D.; Ismagilov, R. F. Angew. Chem., Int. Ed. 2003, 42, 768-772) how multiphase fluid flow in microchannels can be used to address both problems by transporting the reagents inside aqueous droplets (plugs) surrounded by an immiscible fluid. Here, this droplet-based microfluidic system was used to extract kinetic parameters of an enzymatic reaction. Rapid single-turnover kinetics of ribonuclease A (RNase A) was measured with better than millisecond resolution using sub-microliter volumes of solutions. To obtain the single-turnover rate constant (k = 1100 +/- 250 s(-1)), four new features for this microfluidics platform were demonstrated: (i) rapid on-chip dilution, (ii) multiple time range access, (iii) biocompatibility with RNase A, and (iv) explicit treatment of mixing for improving time resolution of the system. These features are discussed using kinetics of RNase A. From fluorescent images integrated for 2-4 s, each kinetic profile can be obtained using less than 150 nL of solutions of reagents because this system relies on chaotic advection inside moving droplets rather than on turbulence to achieve rapid mixing. Fabrication of these devices in PDMS is straightforward and no specialized equipment, except for a standard microscope with a CCD camera, is needed to run the experiments. This microfluidic platform could serve as an inexpensive and economical complement to stopped-flow methods for a broad range of time-resolved experiments and assays in chemistry and biochemistry.  (+info)

Rethinking gamete/embryo isolation and culture with microfluidics. (7/884)

IVF remains one of the most exciting modern scientific developments and continues to have a tremendous impact on people's lives. Since its beginnings, scientists have studied and critically analysed the techniques in order to find ways to improve outcomes; however, little has changed with the actual technology and equipment of IVF. Semen is still processed in test tubes and fertilization and culture still occurs in culture dishes. New technological possibilities exist with the burgeoning advancement of microfluidic technology. Microfluidics is based on the behaviour of liquids in a microenvironment. Although a young field, many developments have occurred which demonstrate the potential of this technology for IVF. In this review, we briefly discuss the physical principles of microfluidics and highlight some previous utilizations of this technology, ranging from chemical analysis to cell sorting. We then present the designs and outcomes for microfluidic devices utilized thus far for each step in IVF: gamete isolation and processing, fertilization, and embryo culture. Finally, we discuss and speculate on the ultimate goal of this technology--development of a single, integrated unit for in-vitro assisted reproduction techniques.  (+info)

A new tool for routine testing of cellular protein expression: integration of cell staining and analysis of protein expression on a microfluidic chip-based system. (8/884)

The key benefits of Lab-on-a-Chip technology are substantial time savings via an automation of lab processes, and a reduction in sample and reagent volumes required to perform analysis. In this article we present a new implementation of cell assays on disposable microfluidic chips. The applications are based on the controlled movement of cells by pressure-driven flow in microfluidic channels and two-color fluorescence detection of single cells. This new technology allows for simple flow cytometric studies of cells in a microfluidic chip-based system. In addition, we developed staining procedures that work "on-chip," thus eliminating time-consuming washing steps. Cells and staining-reagents are loaded directly onto the microfluidic chip and analysis can start after a short incubation time. These procedures require only a fraction of the staining reagents generally needed for flow cytometry and only 30,000 cells per sample, demonstrating the advantages of microfluidic technology. The specific advantage of an on-chip staining reaction is the amount of time, cells, and reagents saved, which is of great importance when working with limited numbers of cells, e.g., primary cells or when needing to perform routine tests of cell cultures as a quality control step. Applications of this technology are antibody staining of proteins and determination of cell transfection efficiency by GFP expression. Results obtained with microfluidic chips, using standard cell lines and primary cells, show good correlation with data obtained using a conventional flow cytometer.  (+info)

Droplet-based microfluidics manipulate discrete volumes of fluids in immiscible phases with low Reynolds number and laminar flow regimes. Interest in droplet-based microfluidics systems has been growing substantially in past decades. Microdroplets offer the feasibility of handling miniature volumes (μl to fl) of fluids conveniently, provide better mixing, encapsulation, sorting, sensing and are suitable for high throughput experiments. Two immiscible phases used for the droplet generation are referred to as the continuous phase (medium in which droplets are generated) and dispersed phase (the droplet phase). The size of the generated droplets is mainly controlled by the flow rates of the continuous phase and dispersed phase, interfacial tension between two phases and the geometry used for the droplet generation. Generally, three types of microfluidic geometries are utilized for the droplet generation: (i) T-Junction, (ii) Flow Focusing, and (iii) Co-Flowing. The benefits of microfluidics can be ...
Miniaturization has been a driving force in many areas of science and technology, most notably in the electronics industry. Droplet-based microfluidics, a methode to produce emulsion drops in a controlled way and at high-throughputs, enables the miniaturization and automation of biological and chemical experiments. Each emulsion drop is used as a closed reaction vessel, enabling the performance of thousands of experiments per second, at throughputs traditional technologies cannot meet. Droplet-based microfluidics is a driving technology in the advances of genomics, proteomics, single-cell analysis, high-throughput screening, and diagnostics. Crucial for using emulsion drops as reaction vessels is that they do not break and that they are tight, not allowing material to be exchanged between drops. To prevent emulsion drops from coalescing, they can be stabilized with surfactants. They adsorb at the liquid-liquid interface, lower the interfacial tension, and add steric stability. For most drop-based
Microfluidics based Lab-on-a-chip technology exhibits an unprecedented perspective in studying microbiology and biochemistry. With microfluidic technology, researchers can manipulate and probe individual cells, and can precisely control their microenvironments. Thus microfluidics enables quantitative measurements with high biological/chemical selectivity and sensitivity, as well as high temporal and spatial resolution. This IDI proposal envisages Western becoming a national leader in multidisciplinary research and education in bionanotechnology and related subjects. As a part of the Westerns overall efforts to accomplish this, the research team plans to apply microfluidic technology to answer essential microbiology questions.. The combination of microfluidics and microbiology also opens a brand new field for education, specifically, bionanotechnology. In the aspect of education, the research team aims:. ...
Lab-on-chips (Microfluidics) market research report covering industry trends, market share, market growth analysis and projection by MIcroMarketMonitor.com. Lab-on-chips (Microfluidics) market report includes,|Key question answered| What are market estimates and forecasts; which of Lab-on-chips (Microfluidics) markets are doing well and which are not? and |Audience for this report| Lab-on-chips (Microfluidics) companies.
View more ,Bioaffinity mass spectrometry screening is a novel approach using non-denaturing electrospray ionization (ESI) mass spectrometry (MS) in identifying drug leads. This screening technique can detect and preserve noncovalent protein-active drug ligand complexes under different physiological conditions. Although there are many successful screening campaigns employing this technique, the big challenge of the screening is the reduction of sample volume needed. We demonstrate in this paper that analysis of samples can be performed using droplet-based microfluidics. Droplets of samples to be screened are formed and delivered directly into the electrospray emitter of a Fourier Transform mass spectrometer. The results show that a MS instrument with a conventional ESI source can clearly detect the samples and distinguish it with the separating oil phase. The proposed technique opens the possibility of bioaffinity mass spectrometry screening of small samples with a simple microfluidic device ...
Identification of rare cells or molecules from a mixture population is important in biology such as identification of rare cancer cells or nucleic acid in early stage cancer diagnosis. Recent advances in droplet-based microfluidics and hydrogel barcoded microsphere to capture all the mRNA molecules in each cell in a single step enables scientists to identify cells based on their whole transcriptome information. However, due to the large number of sequencing reads required to cover the whole transcriptome, this limits the number of cells processed in one sequencing run. We address this problem by using a stepwise approach by first encapsulating single cell and lysis buffer together in a water-in-oil picoliter droplet, then amplifying only the target DNA/RNA molecule of interest in each droplet, pico-inject hydrogel barcoded microsphere into each droplet to tag the amplicons prior to next generation sequencing. We demonstrated the use of this technology by applying it to study how single tumor ...
Adam R. Abate and David A. Weitz Syringe-vacuum microfluidics: A portable technique to create monodisperse emulsions Entry by [[Fei Pu]], AP 225, Fall 2012 Keywords: [[microfluidics]], [[emulsions]], [[monodisperse]] ==Summary== Monodisperse drop formation is the central operation in droplet-based microfluidics but can be quite challenging due to the need for precise, steady pumping of reagents; forming monodisperse drops with controlled properties is thus a stringent demonstration of the effectiveness of a control system.A simple method for creating monodisperse emulsions with microfluidic devices is presented. Unlike conventional approaches that require bulky pumps, control computers,and expertise with device physics to operate devices, this method requires only the microfluidic device and a hand-operated syringe. The fluids needed for the emulsion are loaded into the device inlets, while the syringe is used to create a vacuum at the device outlet; this sucks the fluids through the ...
This high-quality international symposium will bring together leading experts in all fields of droplet-based microfluidics from all around the world. We fully believe that all the presentations in this symposium will stimulate the exchange of ideas and experiences amongst all the participants. There will be four sequential sessions during the course of the two days with plenty of opportunities for discussion and interaction. The topics of the whole symposium include: ...
TY - GEN. T1 - Holographic control of droplet microfluidics. AU - Cordero, Maria-Luisa. AU - Burnham, Daniel R.. AU - Baroud, Charles N.. AU - McGloin, David. PY - 2008. Y1 - 2008. N2 - Droplet microfluidics is an emerging area in miniaturisation of chemical and biological assays, or lab-on-a-chip devices. Normally consisting of droplets flowing in rigid microfluidic channels they offer many advantages over conventional microfluidic design but lack any form of active control over the droplets. We present work, using holographic beam shaping, that allows the real time reconfigurability of microfluidic channels allowing us to redirect, slow, stop, and merge droplets with diameters of approximately 200 microns. A single beam is be sufficient to perform simple tasks on the droplets but by using holographic beam shaping we can produce multiple foci or continuous patterns of light that enable a far more versatile tool.. AB - Droplet microfluidics is an emerging area in miniaturisation of chemical ...
Electrophoresis.. 12.1 Introduction.. 12.2 Experimental Section.. 12.3 Results and Discussion.. 12.4 Applications.. 12.5 Conclusions.. 13 Chemical Separations in 3D Microfluidics.. 13.1 Introduction.. 13.2 Fabrication.. 13.3 Results and Discussion on 3D Valves.. 13.4 Microfluidic Three-Dimensional Separation Columns.. 13.5 Results on Liquid Chromatography.. 13.6 Conclusions.. 14 Enabling Fundamental Research in Proteomics.. 14.1 Introduction.. 14.2 Membrane Protein Extraction.. 14.3 Conclusion.. PART IV BIOMEDICAL APPLICATIONS OF MICROFLUIDICS.. 15 Microengineering Neural Development.. 15.1 Introduction.. 15.2 Microengineering Guidance of Axons to their Targets.. 15.3 Synaptogenesis on a Microfluidic Chip.. 15.4 Conclusions.. 16 Applications of Centrifugal Microfluidics in Biology.. 16.1 Introduction.. 16.2 Why Use Centrifugal Force for Fluid Manipulation?. 16.3 How Centrifugal Microfluidic Platforms Work.. 16.4 CD Applications.. 16.5 Conclusions.. 17 Microfluidic Techniques for Point-of-Care In ...
TY - JOUR. T1 - Merging microfluidics and sonochemistry: towards greener and more efficient micro-sono-reactors. AU - Fernandez Rivas, David. AU - Cintas, P.. AU - Gardeniers, Johannes G.E.. PY - 2012. Y1 - 2012. N2 - Microfluidics enable the manipulation of chemical reactions using very small amounts of fluid, in channels with dimensions of tens to hundreds of micrometers; so-called microstructured devices, from which the iconic image of chips emerges. The immediate attraction of microfluidics lies in its greenness: use of small quantities of reagents and solvents, and hence less waste, a precise control of reaction conditions, integration of functionality for process intensification, safer and often faster protocols, reliable scale-up, and possibility of performing multiphase reactions. Among the limitations found in microfluidics the facile formation of precipitating products should be highlighted, and in this context, the search for efficient mass and energy transfers is a must. Such ...
An integrated circuit device includes a plurality of dynamic array sections, each of which includes three or more linear conductive segments formed within its gate electrode level in a parallel manner to extend lengthwise in a first direction. An adjoining pair of dynamic array sections are positioned to have co-located portions of outer peripheral boundary segments extending in the first direction. At least one of the linear conductive segments within the gate electrode level of a given dynamic array section is a non-gate linear conductive segment that does not form a gate electrode of a transistor. The non-gate linear conductive segment of either of the adjoining pair of dynamic array sections spans the co-located portion of outer peripheral boundary segment toward the other of the adjoining pair of dynamic array sections, and is contained within gate electrode level manufacturing assurance halo portions of the adjoining pair of dynamic array
Microfluidics is a field in which very small volumes of fluids are manipulated with microfabricated structures. At this very small scale, liquids exhibit a low Reynolds number, which is a parameter that describes whether the liquid moves in ordered sheets (as is the case in microfluidics) or if the flow is more chaotic. Because the fluid flow profiles in microfluidic devices can be precisely controlled, they can be engineered to carry out chemical reactions and mixing/separation of particles. Applications are manifold; some include assay development, cell sorting, in vitro modeling, and drug screening.. We are currently developing microfluidics to purify and analyze biomarkers for the early detection of pancreatic cancer. Because pancreatic cancer often does not present with symptoms until it is too advanced to treat, it is crucial to diagnose early. Unfortunately the current method of diagnosing pancreatic cancer is not trivial: an invasive biopsy of the tissue has to be examined by a ...
Plasma is a host of various analytes such as proteins, metabolites, circulating nucleic acids (CNAs), pathogens. The key process of plasma extraction is to eliminate the contamination from blood cells. Conventional methods, such as centrifugation and membrane filtration, are generally lab-intensive, time consuming and even dangerous. In this study, we report an integrated microfluidic device that combines inertial microfluidics and membrane filter. The integrated microfluidic device was evaluated by the diluted (x1/10, x1/20) whole blood, and the quality of the extracted blood plasma was tested. It was found that quality of extracted blood plasma from integrated device was equivalent to that obtained by the centrifugation. This study demonstrates a significant progress towards the practical application of inertial microfluidics with membrane filter for high-throughput and high efficient blood plasma extraction ...
Last week some of us had the pleasure of visiting Boston for the 2012 Workshop on Capillary-based Microfluidics for Bioanalysis, hosted by Dr. Cathie Klapperichs group at Boston University. As those of you who were there or watched the live stream [1] know, there was an incredible line-up of some of the best ideas that paper microfluidics has to offer. This community is using these paper technologies to address a huge range of problems, from identification of counterfeit drugs [2] to inexpensive solar power [3]. Inexpensive and easy-to-use devices are improving our ability to detect antibiotic resistance [4] and monitor water quality [5], while on-paper electrochemical detection [6] and nucleic acid amplification [7] increase the sensitivity achievable in these paperfluidic devices. We heard about the challenges and successes involved when testing new devices in the field [8] and got to experience do-it-yourself fabrication of little devices [9] and tests for TB drug adherence [10].. I didnt ...
Microfluidics is the science and technology that deals with the flow of liquids from microliters (mL) to picoliters (pL) inside a micrometer sized channels1. The ability of microfluidics to miniaturize and mimic various laboratory procedures with limited space, great efficiency and low sample volumes these systems find use in various applications involving continuous flow microfluidics, droplet-based microfluidics, DNA chips (microarrays), molecular biology, etc.. A microfluidic chip is a set of microchannels molded into materials such as glass, silicon or polymers like polydimethyl siloxane (PDMS) 1.. Microfluidic platforms allow physicians to analyze a single drop of blood or urine sample for various markers of diseases without the need of sending comparatively large volumes of these samples to external laboratories for analysis2.. To determine a disease state, microfluidic sensors require specific disease-detecting biomolecules to be inserted into the platform of the system. These ...
With the increasing use of microfluidics, there is a need for a rather general experimental approach in order to monitor and characterize transport effects. Indeed, micro-fabrication methods have allowed the inclusion of numerous new structures and devices within microfluidics channels, and such alterations in flow patterns should impact solute transport characteristics. In the present contribution, Raman microscopy is combined with band-target entropy minimization analysis (BTEM) in order to rapidly assess and map concentration profiles in various regions of a microfluidics device. Two isotopomers, CHCl,sub,3,/sub, and CDCl,sub,3,/sub,, are contacted under laminar conditions. Special consideration is given to the point of contact between the two liquids, transport in straight sections, transport in curved sections, and wall effects. Break-through curves confirmed that stagnation of fluid at the wall is not occurring, despite substantial wall roughness. Since the methods used in the present ...
Turbulence is commonly viewed as a type of macroflow, where the Reynolds number (Re) has to be sufficiently high. In microfluidics, when Re is below or on the order of 1 and fast mixing is required, so far only chaotic flow has been reported to enhance mixing based on previous publications since turbulence is believed not to be possible to generate in such a low Re microflow. There is even a lack of velocimeter that can measure turbulence in microchannels. In this work, we report a direct observation of the existence of turbulence in microfluidics with Re on the order of 1 in a pressure driven flow under electrokinetic forcing using a novel velocimeter having ultrahigh spatiotemporal resolution. The work could provide a new method to control flow and transport phenomena in lab-on-a-chip and a new perspective on turbulence.
9781596931343: Pressure-Driven Microfluidics, Vaclav Tesar - Microfluidics in an emerging technological area involving the handling of small fluid flows in channels and devices so small that a whole system, capable of performing complex tasks, can be held
Microfluidics, which is classified as either active or passive, is capable of separating cells of interest from a complex and heterogeneous sample. Active methods utilise external fields such as electric, magnetic, acoustic, and optical to drive cells for separation, while passive methods utilise channel structures, intrinsic hydrodynamic forces, and steric hindrances to manipulate cells. However, when processing complex biological samples such as whole blood with rare cells, separation with a single module microfluidic device is difficult. Hybrid microfluidics is an emerging technique, which utilises active and passive methods whilst fulfilling higher requirements for stable performance, versatility, and convenience, including (i) the ability to process multi-target cells, (ii) enhanced ability for multiplexed separation, (iii) higher sensitivity, and (iv) tunability for a wider operational range. This review introduces the fundamental physics and typical formats for subclasses of hybrid microfluidic
Advances in Microfluidics and Nanofluidics is an international and interdisciplinary conference with special focus on research activities in the Pacific Rim. It will be held at the beautiful campus of HKUST (http://www.ust.hk/eng/index.htm) by the bay. The last decade has seen exponential growths in microfluidic and nanofluidic research in Asia, driven by robust funding with expectation that it will spur a large Asian biotechnology industry. The intent of this conference is to bring researchers of different disciplines and nationalities together, which is necessary for the Asian community to advance to the next level. It is also an opportunity to expose Asian research achievements to leaders in the field and for Asian students to interact with them. Biomicrofluidics (http://bmf.aip.org), an American Institute of Physics journal, will be the affiliated journal to fascilitate the missions of this conference, and subsequent follow-up conferences.. The invited speakers are leading microfluidics and ...
Microfluidics technology has contributed to powerful tools that have helped advance many areas of biology. The applications of microfluidic systems in chemistry and biochemistry have increased in number over the years with the development of new components and processes for the injection, mixing, pumping, and storing of fluids in the microchannels.
Generally, glass-based microfluidics with the chemical inertness and thermal stability have widely used, while plastics including PDMS have showed their convenience in fabricating microfluidic devices. Therefore, one can simply anticipate that it must be desirable to combine the reliable properties of the glass material and the economic processibility of the plastics. In this point of view, it is an interesting fact that most preceramic polymers convert into the unique inorganic and organic hybrid phase. Moreover, the polymers have been fabricated using near-net shape processing techniques. In this report we have described the fabrication and microfluidic application of preceramic polymer-derived transparent, solvent resistant, thermally stable and bio-compatible microchannels and substrates. The simple micromolding technique was used to fabricate channels by thermal and photo crosslinking of a commercially available poly(vinyl silazane) or hydridopolycarbosilane. Both thermal and photo cured polymer
Coalescence of two kinds of pre-processed droplets is necessary to perform chemical and biological assays in droplet-based microfluidics. However, a robust technique to accomplish this does not exist. Here we present a microfluidic device to synchronize the reinjection of two different kinds of droplets and
The project would include but not be limited to applications of single molecule spectroscopy to understanding of protein folding reactions. Candidates should have a recent Ph.D. in engineering, chemistry, physics, materials science or a related field. The work will involve design, fabrication & characterization of the microfluidics devices and their applications. The candidate would have access to research facilities at both at Center for Biophotonics S&T at UC Davis and at Lawrence Livermore National Laboratory. Opportunities also exist for close collaboration with Single Molecule Biophysics Group at UCLA http://smb.chem.ucla.edu/ and with the laboratory of Prof. L. Lapidus at Michigan State (http://www.pa.msu.edu/~lapidus/introduction.htm). Experience in as many of the following disciplines is not necessary but it would be a plus: computational fluid dynamics, micro- and nano-fabrication, microfluidics, fluorescence microscopy. The ideal candidate would be a person with experience in one of ...
The integration of membranes in microfluidics has attracted significant interest over the last two decades to offer precise mass transport for filtration, extraction, and gas-liquid exchange.1,2 One of the most important attributes to the mass transport of membranes for selective subjects is semi-permeability. The transport is based on a difference in chemical potential between the two sides of a semi-permeable membrane that allows selective subjects to diffuse through.3 Since the membrane porosity governs the transport, it is highly desired that the porosity can be actively tuned and customized to enhance the implementation of integrated membranes. Many approaches have been employed to integrate membranes into microfluidics, including direct incorporation of commercially available membranes, membrane preparation as part of the chip fabrication process, in situ preparation of membranes, and the direct use of the membrane properties of a bulk chip material.3 Among them, the in situ biofabrication ...
TY - JOUR. T1 - Application of microfluidics in waterborne pathogen monitoring. T2 - A review. AU - Bridle, Helen. AU - Miller, Brian. AU - Desmulliez, Marc P. Y.. N1 - HB would like to acknowledge The Royal Academy of Engineering/EPSRC for her research fellowship. BM would like to acknowledge his BBSRC Industrial Case Studentship, supported by Scottish Water. Both HB and MD would like to acknowledge EU funding for the project AQUAVALENS: protecting the health of Europeans by improving methods for the detection of pathogens in drinking water and water used in food preparation. PY - 2014/5/15. Y1 - 2014/5/15. N2 - A review of the recent advances in microfluidics based systems for the monitoring of waterborne pathogens is provided in this article. Emphasis has been made on existing, commercial and state-of-the-art systems and research activities in laboratories worldwide. The review separates sample processing systems and monitoring systems, highlighting the slow progress made in automated ...
Studying microfluidics is simple with this software. Model single- and two-phase creeping flow, electrophoretic, electrokinetic, electroosmotic behavior…
Studying microfluidics is simple with this software. Model single- and two-phase creeping flow, electrophoretic, electrokinetic, electroosmotic behavior…
Health, ... New Academic Research Collaboration (ARC) program makes cutting-edg...Newton MA (PRWEB) May 17 2010 -- Microfluidics (OTCBB: MFLU) the ex...,Microfluidics,Promotes,Scientific,University,Research,Using,Nanotechnology,with,Innovation,Incentives,,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
Bacterial chemotaxis, a remarkable behavioral trait which allows bacteria to sense and respond to chemical gradients in the environment, has implications in a broad range of fields including but not limited to disease pathogenesis, in-situ bioremediation and marine biogeochemistry. And therefore, studying bacterial chemotaxis is of significant importance to scientists and engineers alike. Microfluidics has revolutionized the way we study the motile behavior of cells by enabling observations at high spatial and temporal resolution in carefully controlled microenvironments. This thesis aims to explore the potential of microfluidic technology in studying bacterial behavior by investigating different aspects of bacterial chemotaxis on a microfluidic platform. We quantified population-scale transport parameters of bacteria using videomicroscopy and cell tracking in controlled chemoattractant gradients. Previously, transport parameters have been derived theoretically from single-cell swimming behavior ...
Dolomite, a world leader in microfluidic applications, is pleased to announce the launch of its Membrane Chip Interface, a novel device designed specifically for in-line liquid-liquid separation, liquid-liquid contacting, dead-end and cross-flow filtration, and electrochemistry applications.
Dielectric particles in a non-uniform electric field are subject to a force caused by a phenomenon called dielectrophoresis (DEP). DEP is a commonly used technique in microfluidics for particle or cell separation. In comparison with other separation methods, DEP has the unique advantage of being label-free, fast, and accurate. It has been widely applied in microfluidics for bio-molecular diagnostics and medical and polymer research. This review introduces the basic theory of DEP, its advantages compared with other separation methods, and its applications in recent years, in particular, focusing on the different electrode types integrated into microfluidic chips, fabrication techniques, and operation principles.
RPA and microfluidics: A team at the University of Southampton has developed a digital microfluidic chip device that exploits RPA and on-chip fluorescence to quantitatively detect antimicrobial resistance genes in DNA extracted from patient samples.
TY - THES. T1 - Apoptosis chip for drug screening. AU - Wolbers, F.. N1 - The research described in this thesis was supported by Medisch Spectrum Twente, Hospital Group, Enschede, The Netherlands. PY - 2007/6/8. Y1 - 2007/6/8. N2 - This thesis describes the process of the development towards a microfluidic platform to perform apoptosis studies on chip in real-time at a single-cell level. The requirements for such cell-based assays are discussed as well as the advantages of using microfluidics for cellular analysis. Our main interest is focused on breast cancer cells. Ultimately, this microfluidic chip has to function as a new analytical device for the clinic to provide patients with the best individual therapy.. AB - This thesis describes the process of the development towards a microfluidic platform to perform apoptosis studies on chip in real-time at a single-cell level. The requirements for such cell-based assays are discussed as well as the advantages of using microfluidics for cellular ...
Microfluidics is still a fast growing field and an interesting market, which increasingly demands sophisticated equipment and specific engineering solutions. Aside from the widely discussed chip technology, the external equipment and machinery to operate such a microfluidic chip system comes into focus. A number of companies offer solutions to pursue the various requests made by the microfluidic community. Commercially available systems for pumping fluids are versatile but also highly expensive. Here, we present a fully-programmable pressure source, which is low-cost and can be utilized for pressure-controlled fluid driving, destructive bonding tests, and other pressure-relevant experiments. We evaluated our setup and compared the performance to a commercially available system. Furthermore, we demonstrated the use of the system in the field of droplet microfluidics as a possible application. Our development aims to lower the entrance threshold for microfluidic technology and make it more accessible to a
COC & COP microfluidic cartridge. At microLIQUID we have 10 years of experience working side by side with our customers, manufacturing microfluidic products based on hard polymers such as COC (cyclic olephine copolymer) and COP (cyclic olephine polymer). Our state-of-the-art micro-manufacturing processes for these types of microfluidic cartridges are unique in the industry.. These medical-grade plastics are perfect for microfluidics, as they are outstanding performers in different life science applications: molecular diagnostics, immunoassays, NGS, cell culture and cell diagnostics. These highly biocompatible copolymers have exceptional optical properties, including high clarity and high light transmissivity, in addition to very low moisture absorption, very low permeability to water vapor, and good chemical resistance.. We apply different high-skill engineering processes to our in-house manufacture of COC and COP microfluidic devices, undertaking the complete workflow from initial concept to ...
Technology advances have driven a genomics revolution with sweeping impact on our understanding of life processes. Nevertheless, the arguably more important proteomics revolution remains unrealized. Proteins are complex; meaning that multiple physicochemical properties must be assayed. Consequently, proteomic studies are resource intensive and data limited. To drive a bold transformation of biomedicine, engineering innovation in proteomics instrumentation is needed. While microfluidic technology has advanced separations science, progress lags in the multi-stage separations that are a hallmark of proteomics. This talk will summarize new microengineering design strategies for critical multi-stage protein assays. Specifically, I will introduce our tunable photopatterned materials for switchable function, microfluidic architectures for seamless integration of discrete stages, and multiplexed readouts for quantitation. In a translational example, I will detail assay and design advances from our ...
MreB, an actin-like cytoskeleton in E. coli encoded by MreB gene, is essential for the maintenance of bacterial cell shape. Our project is aimed at the cell-free synthesis of MreB, and the actin homolog MreB was further characterized through both microfluidics technology and nanobiotechnology. As in vivo, MreB is rigidly linked to the cell wall. Here, MreB was cell-free expressed in vitro. Protein localization on the inner oil film could be observed in the W/O/W microdroplets produced with microfluidic technologies, which mimics the cell membrane to provide a non-polar environment. Furthermore, CNTs (carbon nanotubes) were involved as scaffolds for the auxiliary expression of actin homolog MreB. The combination of MreB with CNTs and protein aggregation were observed though the AFM (atomic force microscope) in vitro. Our research cell-free synthesizes the actin-like cytoskeleton filaments, and provides a sound microenvironment for protein synthesis, which helps to recognize, explore and create ...
Single-cell transcriptomic profiling is rapidly advancing in throughput while its cost declines thanks to innovative single cell manipulation technologies, clever barcoding, and next generation sequencing. Microfluidics recently expanded throughput by enabling isolation, lysis, and RNA capture in continuous trains of aqueous nanoliter droplets, making it possible to profile ,10,000 single cells in one experiment. Unfortunately, a focus on fresh cells left behind the vast collection of potentially informative frozen tissue samples. Nuclei from archived samples are teeming with RNA transcripts that have been successfully sequenced with low throughput techniques. Now, Habib and colleagues leverage the scalability of droplet-based microfluidics to perform high-throughput single-nucleus RNA-Seq on frozen archived mouse and human samples in a method they call DroNc-Seq.. They began by optimizing microfluidic channel dimensions to maximize the number of transcripts detected and minimize the occurrence ...
Book Description Amazon Link , CRC Press Link Author: Liang-Yin Chu, Wei Wang ISBN: 978-3-527-34182-5 336 pages June 2017 Summary An increasing number of technologies are [more...] ...
Browse Full Report @ https://www.reportsandmarkets.com/reports/1208363-global-microfluidics-technologies-market-growth-trends-forecasts-2017-2022 The Globa
Dr. Holger Becker is co-founder and CSO of microfluidic ChipShop GmbH. He obtained physics degrees from the University of Western Australia/Perth and the University of Heidelberg. He started to work on miniaturized systems for chemical analysis during his PhD-thesis at Heidelberg university, where he obtained his PhD in 1995. Between 1995 and 1997 he was a Research Associate at Imperial College with Prof. Andreas Manz. In 1998 he joined Jenoptik Mikrotechnik GmbH. Since then, he founded and led several companies in the field of microsystem technologies in medicine and the life sciences. He lead the Industry Group of the German Physical Society between 2004 and 2009 and is Conference Chair for the SPIE Microfluidics, BioMEMS and Medical Microsystems conference as well as acting as regular reviewer of project proposals on national and EU level and for several journals devoted to microsystem technologies. ...
Cell factories are cells that have been engineered to produce a compound of interest, ranging from biopharmaceuticals to biofuels. With advances in metabolic engineering, the number of cell factory variants to evaluate has increased dramatically, necessitating screening methods with increased throughput. Microfluidic droplets, which can be generated, manipulated and interrogated at very high throughput, are isolated reaction vessels at the single cell scale. Compartmentalization maintains the genotype-phenotype link, making droplet microfluidics suitable for screening of extracellular traits such as secreted products and for screening of microcolonies originating from single cells.. In Paper I, we investigated the impact of droplet microfluidic incubation formats on cell culture conditions and found that syringe and semi open incubation resulted in different metabolic profiles. Controlling culture conditions is key to cell factory screening, as product formation is influenced by the state of the ...
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The behavior of fluids on the microscale may differ from macrofluids in factors such as surface tension, energy dissipation, and fluidic resistance at the beginning to dominate the system. Microfluidics studies these behavioral changes and how they can be avoided or exploited for new uses.. On a small scale (diameters between 100 nanometers and several hundred micrometers, approximately) some properties of special interest appear. The Reynolds number, which characterizes the presence of turbulent flow, is extremely low, so laminar flow will be maintained. In this way, joining two fluids could not easily mix through turbulence, so the diffusion must be done by itself in the two fluids to be mixed.. ...
MicruX is focused on the design, development and manufacture of novel miniaturized analytical systems based on Lab-on-a-Chip, microfluidics and electrochemistry.
TY - JOUR. T1 - Multiplex analysis of enzyme kinetics and inhibition by droplet microfluidics using picoinjectors. AU - Sjostrom, Staffan L.. AU - Jönsson, Håkan. AU - Svahn, Helene Andersson. PY - 2013. Y1 - 2013. N2 - Enzyme kinetics and inhibition is important for a wide range of disciplines including pharmacology, medicine and industrial bioprocess technology. We present a novel microdroplet-based device for extensive characterization of the reaction kinetics of enzyme substrate inhibitor systems in a single experiment utilizing an integrated droplet picoinjector for bioanalysis. This device enables the scanning of multiple fluorescently-barcoded inhibitor concentrations and substrate conditions in a single, highly time-resolved experiment yielding the Michaelis constant (Km), the turnover number (kcat) and the enzyme inhibitor dissociation constants (ki, ki′). Using this device we determine Km and kcat for β-galactosidase and the fluorogenic substrate Resorufin β-d-galactopyranoside ...
Laboratory of microfluidic technologies for biomedicine: MicroRNA, miRNA, organ-on-chip, microfluidics, toxin, viscumin, ricin, laminin, placenta, intestine, gut, liver, brain, blood-brain barrier, cancer, tumor
University of Tennessee at Knoxville Doctoral student, Peter Golden Shankles, presents his dissertation on Interfacing to Biological Systems Using Microfluidics, discussing the popular new field of microfluidics and the 3D printed tools that are propelling it forward-for this project and numerous others recently too. 3D printing allows for much greater self-sustainability in the lab as researchers can create tools for experiments and chemical reactions on their own, but attention must also be paid to how such technologies affect chemical transformations and influence biological systems.. As microfluidics are used more frequently with cell-free protein synthesis systems (CFPS), researchers usually set up tubes to experiment with reactions. As the author points out, this is usually easy, but other studies have shown better success with engineered reaction hardware. In this study, the researchers aim to begin using microfluidics as well as nanoscale membranes to lessen distances in diffusion ...
Microfluidic devices have a wide variety of biological applications. My Ph.D. dissertation focuses on three major projects. A) culturing a non-adherent immortal cell line within a microfluidic device under static and dynamic media flow conditions; B) designing and fabricating novel microfluidic devices for electrokinetic injecting analytes from a hydrodynamic fluid; and C) using this novel injection method to lyse single non-adherent cells by applying a high electric field across the cell at a microfluidic channel intersection. There are several potential advantages to the use of microfluidic devices for the analysis of single cells: First, cells can be handled with care and precision while being transported in the microfluidic channels. Second, cell culturing, handling, and analysis can be integrated together in a single, compact microfluidic device. Third, cell culturing and analysis in microfluidic devices uses only extremely small volumes of culturing media and analysis buffer. In this ...
Embodiments of the present invention provide improved microfluidic devices and related apparatus, systems, and methods. Methods are provided for reducing mixing times during use of microfluidic devices. Microfluidic devices and related methods of manufacturing are provided with increased manufacturing yield rates. Improved apparatus and related systems are provided for supplying controlled pressure to microfluidic devices. Methods and related microfluidic devices are provided for reducing dehydration of microfluidic devices during use. Microfluidic devices and related methods are provided with improved sample to reagent mixture ratio control. Microfluidic devices and systems are provided with improved resistance to compression fixture pressure induced failures. Methods and systems for conducting temperature controlled reactions using microfluidic devices are provided that reduce condensation levels within the microfluidic device. Methods and systems are provided for improved fluorescent imaging of
misc{8894052, abstract = {Microfluidic applications nowadays have become of great interest due to their broad compatibility especially in biological applications, and one of them being droplet-based cell encapsulation. Cell encapsulation in droplets is carried out by discretising an aqueous phase (i.e. cell suspension) and including them into a continuous oil phase. This methodology is a potential gateway to high throughput droplet-based cell fusion (e.g. for the production of hybridomas). The challenge here is to achieve a high efficiency of correctly paired cells in a droplet to overcome the random fusion pairing during bulk cell fusion. As such, droplet microfluidics can be used to co-encapsulate a single cell A and a single cell B cells in one droplet or encapsulate cells separately and merge droplets with desired cell number and type subsequently with other droplet manipulations. In this study, separate encapsulation studies of human B lymphocytes and mouse embryonic stem cells were ...
The Microfluidics Lab aims to design and fabricate microfluidic devices for clinical applications and to study important cellular issues. With much progress in microfluidics in the last decade, microfluidics has become an enabling technology for miniaturization, or lab-on-a-chip platform. In our Lab, cell-based studies are the focus of research.. The Labs cell culture room (CCR) cultures about 8 cell lines, supplying fresh cells for experiments. The Labs cleanroom provides essential facilities for fabricate of microdevices. Additional microfabrication needs are supported by various centers on campus.. The most important asset of the Lab is people. Current, 2 doctoral students working closely with 4 master students in a team-work atmosphere to mutually support each others research needs. Collaboration with surgeons and doctors in the National Taiwan University Hospital as well as other faculty members complements our engineering know-how to enable important research in the cellular ...
Droplet-based microfluidics has been used to facilitate high throughput analysis of individual prokaryote and mammalian cells. However, there is a scarcity of similar workflows applicable to rapid phenotyping of plant systems. We report on-chip encapsulation and analysis of protoplasts isolated from the emergent plant model Marchantia polymorpha at processing rates of ,100,000 protoplasts per hour. We use our microfluidic system to quantify the stochastic properties of a heat-inducible promoter across a population of transgenic protoplasts to demonstrate that it has the potential to assess gene expression activity in response to environmental conditions. We further demonstrate on-chip sorting of droplets containing YFP-expressing protoplasts from wild type cells using dielectrophoresis force. This work opens the door to droplet-based microfluidic analysis of plant cells for applications ranging from high-throughput characterisation of DNA parts to single-cell genomics ...
Optical coherence tomography (OCT) angiography (OCTA) has been actively studied as a noninvasive imaging technology to generate retinal blood vessel network maps for the diagnoses of retinal diseases. Given that the uses of OCT and OCTA have increased in the field of ophthalmology, it is necessary to develop retinal phantoms for clinical OCT for product development, performance evaluation, calibration, certification, medical device licensing, and production processes. We developed a retinal layer-mimicking phantom with microfluidic channels based on microfluidic fabrication technology using polydimethylsiloxane (PDMS) and titanium dioxide (TiO2) powder. We implemented superficial and deep retinal vessels using microfluidic channels. In addition, multilayered thin films were synthesized with multiple spin-coating processes that comprised layers that corresponded to the retinal layers, including the ganglion cell layer (GCL), inner plexiform layer (IPL), and inner nuclear layer (INL). The phantom ...
The main aim of this project was to develop novel concepts for miniaturization of bioanalytical techniques for investigating biomolecular interactions. We used optical tweezers to selectively address individual biological objects in microfluidic channels. A general introduction of applications of optical tweezers and microfluidics is given in chapter 1. Theoretical concepts related to optical trapping and microfluidics are reviewed in chapter 2, followed by a detailed description of the instrumentation in chapter 3. In chapter 4, ligand-receptor interactions are studied under physiological conditions: whole cells or native vesicles carrying in their membrane the protein of interest are immobilized first in the laser trap inside a microfluidic channel, then the reaction is initiated by changing the solution in the region around the trap. In chapter 5 and chapter 6 respectively, surface-modified polystyrene beads are used to study ligand-receptor interactions and DNA hybridization. The examples of ...
Electrophoretic separation in nanofluidic channels exhibits significant differences with microfluidics. We discuss a theoretical / experimental collaboration investigating particle separation by electropohoresis in nanochannels. Recent experimental results in the laboratory of our collaborator Dr. Pennathur (UCSB, Dept. ME) indicate that increased fidelity can be achieved in separating particles by size and charge when using channels with cross sections of nanometer dimensions (100nm x 1000nm), as opposed to larger microfluidic channels. For short double-strands of DNA (10 - 100 base pairs) it is found that separation in microfluidic channels produces electropherograms with only one lumped peak. However, for nanofluidic channels several clearly distinct peaks are observed. Given the small dimensions of the nanofluidic channel, it is expected that new effects which were relatively weak in microfluidic channels play an important role. Identifying how these underlying mechanisms augment electrophoretic
The purpose of this thesis is to study the crystallization in a microfluidic device of an active pharmaceutical ingredient which is (2S)-2-[(4R)-2-oxo- 4-propylpyrrolidin-1-yl] butanamide, with product name Brivaracetam. This molecule is manufactured by the Belgian pharmaceutical industry UCB, and a better understanding of its crystallization in the microscopic scale, and especially its polymorphism, would lead to new possibilities in order to develop a future industrial continuous crystallizer based in the microfluidic technology. For this purpose, several experiments have been run, both in the macroscopic scale and using the microfluidics technology. The solubility curve for the system was determined, and also a cluster formation was analysed using volumes around 10ml of solution. Several crystallizations were done also with solutions of this volume to first understand the polymorphism that the solute presents. For the micro-scale crystallizations, the microfluidic device used was a system ...
A microfluidic platform for the rapid determination of distribution coefficients by gravity assisted droplet-based liquid-liquid extraction, C. E. Poulsen, R. C. R. Wootton, A. Wolff, A. J. deMello & K. S. Elvira, Analytical Chemistry, 2015, 87, 6265. The aim of this research was to use microfluidic technologies to provide an original improvement to a method that has not been updated in decades, namely the determination of distribution coefficients. The platform provides clear advantages to the traditional shake-flask method (it is 48 times faster, uses 99% less reagents, has very low user sensitivity and higher reproducibility), whilst being as simple as possible: the platform makes use of picolitre-sized droplets to take advantage of the large surface-area-to-volume ratios, but uses a separation chamber that falls in the milli-fluidic regime to enable the use of gravity for phase separation. To allow this platform to be used in commercial settings, it was fabricated using cyclic olefin ...
The widespread dissemination of CTX-M extended spectrum β-lactamases among Escherichia coli bacteria, both in nosocomial and community environments, is a challenge for diagnostic bacteriology laboratories. We describe a rapid and sensitive detection system for analysis of DNA containing the blaCTX-M …
A variety of pulmonary diseases such as COPD, asthma, ARDS are profoundly associated with the surfactant dysfunction that leads to liquid plug formation across the airway lumen [19]. Several animal model studies have shown during such lung disorders severe tissue-level damage to the distal lung airways due to repeated closure and reopening process [4]. To mimic exactly the in vivo conditions, Huh et al. [20] developed a compartmentalized microfluidic airway models and demonstrated that the reopening of occluded microfluidic airway causes severe injury of pulmonary epithelial cells [20]. In the lung airways, rupturing of the liquid plugs leads to abnormal breath sounds known as crackles. To simulate this scenario, a three-dimensional (3D) microfluidic device was developed to detect acoustically the crackling sound and it was demonstrated that there is a higher risk of cell injury when liquid plugs become very thin. They demonstrated cellular level of lung injury under flow condition using this ...
TY - JOUR. T1 - A highly efficient bead extraction technique with low bead number for digital microfluidic immunoassay. AU - Huang, Cheng Yeh. AU - Tsai, Po Yen. AU - Lee, I. Chin. AU - Hsu, Hsin-Yun. AU - Huang, Hong Yuan. AU - Fan, Shih Kang. AU - Yao, Da Jeng. AU - Liu, Cheng Hsien. AU - Hsu, Wen-Syang. PY - 2016/1/1. Y1 - 2016/1/1. N2 - Here, we describe a technique to manipulate a low number of beads to achieve high washing efficiency with zero bead loss in the washing process of a digital microfluidic (DMF) immunoassay. Previously, two magnetic bead extraction methods were reported in the DMF platform: (1) single-side electrowetting method and (2) double-side electrowetting method. The first approach could provide high washing efficiency, but it required a large number of beads. The second approach could reduce the required number of beads, but it was inefficient where multiple washes were required. More importantly, bead loss during the washing process was unavoidable in both methods. ...
The market study on Global Microfluidic Devices Market 2017 Research Report studies current as well as future aspects of the Microfluidic Devices Market primarily based upon factors on which the companies compete in the market, key trends and segmentation analysis. This report covers each side of the worldwide market, ranging from the fundamental market info and advancing more to varied important criteria, based on that, the Microfluidic Devices market is segmented. Microfluidic Devices industry research report analyzes, tracks, and presents the global market size of the major players in every region around the world. Furthermore, the report provides data of the leading market players in the Microfluidic Devices market.. This report studies Microfluidic Devices in Global market, especially in North America, China, Europe, Southeast Asia, Japan and India, with production, revenue, consumption, import and export in these regions, from 2012 to 2017, and forecast to 2022.. Request for FREE Sample ...
By taking advantage of the natural movement of liquid through paper, researchers at Harvards Whitesides Research Group may have found a way to make microfluidics technology much cheaper. The result could be disposable diagnostic tests simple and abundant enough for use in the developing world.. The field of microfluidics deals with the precise manipulation of tiny quantities of liquid. One of its most promising applications is the so-called lab-on-a-chip, which can work with much smaller fluid samples than larger devices require, potentially allowing for more portable diagnostic tools. But existing microfluidic chips are generally made from comparatively expensive materials like silicon, glass, or plastic and have tiny pumps and valves that can be difficult to manufacture.. Now, Harvards George Whitesides and his team have built a microfluidic device on a square of paper the size of a pinky fingernail. Its the first example Ive heard of paper microfluidics, says Albert Folch, a bioengineer ...
Microfluidic devices are analogous to circuit boards, and they can be programmed to perform all kinds of laboratory tasks on a small scale. They have the potential to perform all kinds of medical tests involving body fluids in a short time and using very small samples.. While circuit boards pass electricity, which can be abstracted and quantified as bits, microfluidic devices tend to work with liquids that can mix with one another and contaminate each other. For microfluidic devices to approach the logic abilities of circuit boards, the fluids within have to somehow be perfectly separated from each other until the time that theyre expected to mix. Conventional microfluidic gates and valves arent adequate in this context, so researchers at Duke University have now developed a way to keep individual droplets from touching each other while moving them around using sound waves inside a microfluidic device.. Scaling up this approach could lead to programmable and rewritable microfluidics that can ...
A system and method for integrating microfluidic components in a microfluidic system enables the microfluidic system to perform a selected microfluidic function. A capping module includes a microfluidic element for performing a microfluidic function. The capping module is stacked on a microfluidic substrate having microfluidic plumbing to incorporate the microfluidic function into the system. The microfluidic element may comprise a matrix having an affinity for selected molecules in a sample. The matrix binds, reacts with and/or retains the selected molecules without affecting other molecules in the sample.
A system and method for integrating microfluidic components in a microfluidic system enables the microfluidic system to perform a selected microfluidic function. A capping module includes a microfluidic element for performing a microfluidic function. The capping module is stacked on a microfluidic substrate having microfluidic plumbing to incorporate the microfluidic function into the system. The microfluidic element may comprise a matrix having an affinity for selected molecules in a sample. The matrix binds, reacts with and/or retains the selected molecules without affecting other molecules in the sample.
Health, ...ANN ARBOR Mich. Cancer cells are on the move in the bloodstream in t...In a study of 51 patients researchers used a state-of-the art microfl...The findings published in Gastroenterology suggest that circu... While there is much work that still needs to be done there is great ...,Microfluidic,technology,reveals,potential,biomarker,for,early,pancreatic,cancer,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
Engineering cellular microenvironments that more accurately reflect the in vivo situation is now recognized as being crucial for the improvement of the in vitro viability and in vivo-like function of cells or tissues. Microfluidic technologies have been increasingly applied since the late 1990s for this purpose, with a growing number of examples of perfused cell and tissue cultures in microfluidic chambers and channels. The well-defined solution flows provided by microfluidics mean enhanced cell growth and function through improved nutrient delivery and waste removal. Additional benefits include the implementation of well-defined temporal and spatial (bio)chemical gradients, and mechanical signals that cells experience in their natural environment. Because the ability to culture cells and tissue under such controlled conditions leads to cellular function that is distinctly more organ-like, the microfluidic systems used are now referred to as organs-on-a-chip or microphysiological systems. ...
We previously developed a Deterministic Lateral Displacement (DLD) microfluidic method in silicon to separate cells of various sizes from blood (Davis et al. a microfluidic cell processing protocol that recovered 88% (average) of input WBCs and removed 99.985% (average) of Input erythrocytes (red blood cells) and 99% of unbound mAb in PU-H71 reversible enzyme inhibition 18 min (average). Flow cytometric evaluation of the microchip Product, with no further processing, lysis or centrifugation, revealed excellent forward and side light scattering and fluorescence characteristics of immunolabeled WBCs. These results indicate that cost-effective plastic DLD microchips can speed and automate leukocyte processing for high quality movement cytometry evaluation, and recommend their electricity for multiple additional research and medical applications concerning enrichment or depletion of common or uncommon cell types from bloodstream or tissue examples. ratio for every cell type was determined based on ...
Microlytic was founded in 2006 with the intention to use microfluidic technology to help solve the major problems in structural biology. Microlytic sought to develop and produce microfluidic chips that offered a high probability of crystallizing target proteins with a set-up that is simple and easy to use.. Microlytic developed microfluidic chips to let users grow crystals large enough to be used immediately for X-ray diffraction, plus give users direct access to the crystals themselves. These goals were realized with the development of the Crystal Former - the first microfluidic platform to allow users to go from crystallization screen to structure using a single device.. The advantages of the Crystal Former are ...
In order to overcome these challenges, we aim to develop a novel breast cancer model that incorporates the relevant properties of the three-dimensional microenvironment. For this purpose, we use microfluidic technology, which enables us to manipulate and control fluids at the small scale. Cell encapsulation is used to first generate soft, cell-containing beads that mimic the basement membrane. These beads are then embedded in a more fibrous matrix that mimics the stromal ECM, completing the tissue model.
Video articles in JoVE about microfabrication include Microfabrication of Nanoporous Gold Patterns for Cell-material Interaction Studies, Microfabrication of Chip-sized Scaffolds for Three-dimensional Cell cultivation, Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening, Experimental Methods for Trapping Ions Using Microfabricated Surface Ion Traps, Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks, A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)-Cell Interaction and the Resultant Bioeffects at the Single-cell Level, Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles, Soft Lithographic Procedure for Producing Plastic Microfluidic Devices with View-ports Transparent to Visible and Infrared Light, A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological
Microfluidic technology may offer faster and more controlled ways to produce radiotracers for medical imaging, including studies into Parkinsons disease, thanks to a first of its kind approach in the Southern Hemisphere ...
If you wanted to know if your child had a fever or be certain that the roast in the oven was thoroughly cooked, you would, of course, use a thermometer that you trusted to give accurate readings at any temperature within its range. However, it isnt that simple for researchers who need to measure temperatures in microfluidic systemstiny, channel-lined devices used in medical diagnostics, DNA forensics and lab-on-a-chip chemical analyzersas their current thermometer can only be precisely calibrated for one reference temperature. Now, researchers at the National Institute of Standards and Technology (NIST) have proposed a mathematical solution that enables researchers to calibrate the thermometer for microfluidic systems so that all temperatures are covered.. Reactions taking place in microfluidic systems often require heating, meaning that users must accurately monitor temperature changes in fluid volumes ranging from a few microliters (a droplet approximately 1 millimeter in diameter) ...
The 18-well titer plate works with the spacing of a 96-well microtiter plate namely 9 mm and is available in different materials and in transparent and colored versions It can be used with our adapter frame in microtiter-plate format that is made as a special adapter for microfluidic chips in
Further details of the study can be found in the paper titled 3D-printed bioreactors for DNA amplification: application to companion diagnostics. It is co-authored by A.K. Pantazis, G. Papadakis, K. Parasyris, A. Stavrinidis, and E. Gizeli.. The DNA amplification process comes under microfluidics, a field of study that 3D printing has benefited greatly in recent years (and vice versa). Earlier in 2020, a team of researchers from UC Davis published a paper detailing the development of a new droplet-based 3D printing method using microfluidics. The technique allows the user to manipulate the extruded ink composition and properties in real time, enabling the fabrication of diverse structures. The team expects the technology to excel in soft robotics and tissue engineering.. Elsewhere, in Europe, researchers produced and published a review on 3D printing microfluidic applications. In the review the scientists state that the technology will allow for the creation of a new generation of increasingly ...
Microfluidics is the study of precise control and manipulation of fluids that are geometrically constrained to a small, normally sub millimetre, range. It has application in various fields like engineering, physics, chemistry, biochemistry, nanotechnology and biotechnology, from real applications to the plan of systems in which little volumes of fluids are used to attain multiplexing, automation etc. It has appeared in the beginning of the 1980s and is used in the expansion of inkjet print heads, DNA chips, lab-on-a-chip technology, micro-propulsion and micro-thermal technologies.. ...
Microfluidic Environments and Cell BehaviorThe use of microfluidics for the study of basic biology is still in its infancy with the focus to date on the use of microfluidics for acute analysis. Another possible use of microfluidics and more broadly micro systems, is for the longer term growth, study and even production of living systems. In this presentation, I will describe work towards this end involving the development of a fabrication platform that makes possible integrated organic and biomimetic microsystems. Further, preliminary evidence suggesting that these systems can, in some cases, provide a more nature (or in vivo-like) micro environment for living systems will be described. The effect of microenvironments on the behavior of a variety of living systems (insect cells, yeast, mammalian embryos, human embryonic stem cells and mammary epithelial cells) will be summarized.. April 1, ...
Microfluidics is a young and rapidly expanding scientific discipline, which deals with fluids and solutions in miniaturized systems, the so-called lab-on-a-chip systems. It has applications in chemical engineering, pharmaceutics, biotechnology and medicine.
Microfluidics is a young and rapidly expanding scientific discipline, which deals with fluids and solutions in miniaturized systems, the so-called lab-on-a-chip systems. It has applications in chemical engineering, pharmaceutics, biotechnology and medicine.
Project Description: Grade IV astrocytomas known as glioblastoma multiforme (GBM) are the most aggressive primary brain tumors, with a median survival rate of 15 months after diagnosis. Current methodologies for diagnosis include time-consuming histopathological reviews of biopsied brain tumor tissue to determine malignancy, which can delay early diagnosis and treatment. We propose to use microfluidics in combination with labeled magnetic beads to separate invasive glioma cells from other healthy neural tissue within a few hours. A strategy that combines rapid cell sorting with subsequent cell culture of a pure population of the patients glioma cells could not only speed up time to diagnosis, but also enhance personalized treatment. We hypothesize that incubating magnetic beads labeled with anti-epithelial cell adhesion molecule (epCAM) antibody will allow for the selective separation of glioma cells from other neural tissue cell types from co-culture, as judged by recent evidence that glioma ...
Lin BC. 1996-2012:From Capillary Electrophoresis to Intelligent Control Digital Microfluidics[C]. 见:12th Asia-Pacific International Symposium on Capillary Electrophoresis and Microscale Separation and Analysis. 新加坡. 2012-12-16 ...
Teh, Shia-Yen; Lin, Robert; Hung, Lung-Hsin; Lee, Abraham P. (2008-01-29). "Droplet microfluidics". Lab on a Chip. 8 (2): 198- ... Self-propelled system demonstrate a potential as micro-fluidics devices and micro-mixers. Self-propelled liquid marbles have ...
Chakraborty is known for his fundamental as well as translational research in the domain of microfluidics and nanofluidics. His ... Chakraborty has research interests in the area of Microfluidics and Micro/Nano scale transport processes, including their ... ". "Introduction to Fluid Mechanics and Fluid Engineering (NPTEL)". "Microfluidics (NPTEL)". "Shanti Swarup Bhatnagar Prize". " ...
ISBN 978-0-521-11903-0. Bruus, H. (2007). Theoretical Microfluidics. Oxford University Press. Karniadakis, G.M., Beskok, A., ... Chang, H.C., Yeo, L. (2009). Electrokinetically Driven Microfluidics and Nanofluidics. Cambridge University Press.CS1 maint: ... Li, D. (2004). Electrokinetics in Microfluidics. Academic Press. ...
Sun T, Morgan H (April 2010). "Single-cell microfluidic impedance cytometry: a review". Microfluidics Nanofluidics. 8 (4): 423- ...
Electrokinesis is of considerable practical importance in microfluidics, because it offers a way to manipulate and convey ... Chang, H.C.; Yeo, L. (2009). Electrokinetically Driven Microfluidics and Nanofluidics. Cambridge University Press. Kirby, B.J ... ISBN 978-0-521-11903-0. Bruus, H. (2007). Theoretical Microfluidics. Oxford University Press. Patterson, Michael; Kesner, ...
ISBN 978-0-521-11903-0. Bruus, H. (2007). Theoretical Microfluidics. "microfluidics EO pump". Archived from the original on ... Capillary electrophoresis Electroosmotic flow Glossary of fuel cell terms Microfluidics Micropump Sol-gel Kirby, B.J. (2010). ...
Berthier, J.; Silberzan, P. Microfluidics for Biotechnology. Gomez, F.A. Biological Applications of Microfluidics.[ISBN missing ... Microfluidics and BioMEMS Applications. Microsystems. 10. SpringerLink. 2002. doi:10.1007/978-1-4757-3534-5. ISBN 978-1-4419- ... Ion channel screening (patch clamp) Microfluidics Microphysiometry Organ-on-a-chip Real-time PCR: detection of bacteria, ... 2009). Lab-on-a-Chip Technology: Fabrication and Microfluidics. Caister Academic Press. ISBN 978-1-904455-46-2. Herold, KE; ...
ISBN 978-0-521-11903-0. Bruus, H. (2007). Theoretical Microfluidics. Pfitzner, J. (1976). "Poiseuille and his law" (PDF). ...
PMID 26975219.CS1 maint: multiple names: authors list (link) D. Di Carlo (2009). "Inertial microfluidics". Lab on a Chip. 9 (21 ... microfluidics, and MEMS. The usual techniques of conventional CCD and CMOS cameras are inadequate for capturing fast dynamical ...
273-5, doi:10.1111/j.1365-2044.1976.tb11804.x, PMID 779509, S2CID 40607063 Bruus, Henrik (2007). Theoretical Microfluidics. ...
ISBN 978-0-262-08198-6. Bruus, H. (2007). Theoretical Microfluidics. Kirby, B.J. (2010). Micro- and Nanoscale Fluid Mechanics: ...
ISBN 978-0-9728422-0-4. ,volume= has extra text (help) Bruus, H. (2007). Theoretical Microfluidics. ISBN 978-0-19-923509-4. ... Chang, H.C.; Yao, L. (2009). Electrokinetically Driven Microfluidics and Nanofluidics. Levich, V. (1962). Physicochemical ... Induced-charge Electrokinetics Streaming potential Zeta potential Electroosmotic pump Electrical double layer Microfluidics ...
... s in Droplet-based Microfluidics[edit]. Surfactants play an important role in Droplet-based Microfluidics in the ... "Surfactants in droplet-based microfluidics". Lab on a Chip. 12 (3): 422-433. doi:10.1039/C1LC20582J. ISSN 1473-0189 ...
Frank, Michael; Drikakis, Dimitris (2017-08-24). "Solid-like heat transfer in confined liquids". Microfluidics and Nanofluidics ... Microfluidics and Nanofluidics. 15 (4): 559-574. doi:10.1007/s10404-013-1154-4. ISSN 1613-4990. Frank, Michael; Drikakis, ...
ISBN 978-0-521-11903-0. Li, D. (2004). Electrokinetics in Microfluidics. Academic Press. ISBN 0-12-088444-5. PC Clemmow & JP ...
The commonly used materials for microfluidics suffer from unwieldy steps and often ineffective bonding processes, especially ... IEEE Transducers 2013 Barcelona, 408-411 (2013) [2] MICROFLUIDICS & LAB-ON-CHIP Hoyle, Charles E. (2010). "Thiol-Ene Click ... 21 (2011) 025008 (8pp) [14] 1. Pardon G, et al., Microfluidics and Nanofluidics. 2014 Feb 14. [15] Homola, Chemical Reviews, ... membrane-integrated microfluidics based on replica molding of a thiol-ene epoxy thermoset for organ-on-a-chip applications". ...
Microfluidics and Nanofluidics. 17: 53-71. doi:10.1007/s10404-013-1289-3. S2CID 85525659. Freund, M.; Csikos, R; Keszthelyi, S ...
Stanford Microfluidics Laboratory. 14 (32): 11534-45. Bibcode:2012PCCP...1411534S. doi:10.1039/c2cp42121f. PMID 22806549. ...
Microfluidics and Nanofluidics. 21 (8): 137. doi:10.1007/s10404-017-1974-8. ISSN 1613-4990. S2CID 103813194. Duryodhan, V. S.; ... Microfluidics and Nanofluidics. 22 (2): 19. doi:10.1007/s10404-018-2034-8. ISSN 1613-4990. S2CID 102682551. Shah, Niraj; ...
Microfluidics and Nanofluidics. 17: 53-71. doi:10.1007/s10404-013-1289-3. S2CID 85525659. Dick, William B (1872). "Encyclopedia ...
1952-, Berthier, Jean (2010). Microfluidics for biotechnology. Silberzan, Pascal. (2nd ed.). Boston: Artech House. ISBN ...
Microfluidics and Nanofluidics. 17 (4): 773-779. doi:10.1007/s10404-014-1351-9. S2CID 21701353. Rånby, Bengt (1998). " ...
Shields CW, Ohiri KA, Szott LM, López GP (March 2017). "Translating microfluidics: Cell separation technologies and their ... Martel JM, Toner M (July 2014). "Inertial focusing in microfluidics". Annual Review of Biomedical Engineering. 16 (1): 371-96. ... Microfluidics and Nanofluidics. 15 (5): 611-623. doi:10.1007/s10404-013-1176-y. PMC 3810988. PMID 24187531. Ding X, Lin SC, ... "Ultrahigh-throughput screening in drop-based microfluidics for directed evolution". Proceedings of the National Academy of ...
ISBN 978-0-521-11903-0. Tabeling, Patrick (2006). Introduction to Microfluidics. Oxford University Press. ISBN 978-0-19-856864- ...
3-34, doi:10.1007/978-3-642-34243-1_1, ISBN 9783642342424 E., Rapp, Bastian (2016-12-13). Microfluidics : modeling, mechanics ... 1952-, Berthier, Jean (2010). Microfluidics for biotechnology. Silberzan, Pascal. (2nd ed.). Boston: Artech House. ISBN ...
Fundamentals of Microfluidics. Kirby, B.J. (2010). Micro- and Nanoscale Fluid Mechanics: Transport in Microfluidic Devices. ...
Microfluidics and Nanofluidics. 18 (2): 233-243. doi:10.1007/s10404-014-1423-x. ISSN 1613-4982. S2CID 54087370. Lister, J.R. ( ... Microfluidics and Nanofluidics. 11 (4): 417-428. doi:10.1007/s10404-011-0807-4. ISSN 1613-4982. S2CID 97060677. Myers, T. G.; ...
Microfluidics for Biological Applications. Springer Science & Business Media. p. 102. ISBN 9780387094809. Retrieved 2016-11-20 ...
Microfluidics for Biological Applications. Springer. p. 125. ISBN 978-0-387-09480-9. Jean-Louis Viovy (2000). "Electrophoresis ...
... have been developed to achieve 3D focusing of cells/particles in microfluidics. A pair of interdigital ... Yeo, Leslie Y.; Friend, James R. (2014-01-03). "Surface Acoustic Wave Microfluidics". Annual Review of Fluid Mechanics. 46 (1 ... "Surface acoustic wave microfluidics". Lab on a Chip. Royal Society of Chemistry (RSC). 13 (18): 3626-3649. doi:10.1039/ ...
Examples of open microfluidics include open-channel microfluidics, rail-based microfluidics, paper-based, and thread-based ... Droplet-based microfluidics is a subcategory of microfluidics in contrast with continuous microfluidics; droplet-based ... Open microfluidics[edit]. In open microfluidics, at least one boundary of the system is removed, exposing the fluid to air or ... Bruus, H. (2007). Theoretical Microfluidics. Oxford University Press.. *^ Tabeling, P. (2005). Introduction to Microfluidics. ...
The course provides the Biochemical Engineering student with the necessary knowledge to consider microfluidic and micro-scale approaches for bioprocessing applications. The aims of the course are to familiarise students with microfluidic technology, microfabrication techniques, advanced fluid mechanics, and available microfluidic and micro-scale bioprocessing methods. Expert lectures from industry will provide in-depth understanding of exemplary, commercially available devices and their applications. Practical laboratory sessions will support a hands-on understanding of the topic ...
Microfluidics technology has contributed to powerful tools that have helped advance many areas of biology. The applications of ... New microfluidics device detects malignant multiple myeloma cells in blood. *Novel device shows promise to identify high- ... Microfluidics technology has contributed to powerful tools that have helped advance many areas of biology. The applications of ... Going forward, microfluidics will hopefully play a greater role in developing economical methods for single-cell assays, cell- ...
"Duke Microfluidics Lab". microfluidics.ee.duke.edu. Retrieved 2017-05-22. Kim CJ (November 2001). Micropumping by ... And in contrast to continuous-flow microfluidics, digital microfluidics works much the same way as traditional bench-top ... "Precise droplet volume measurement and electrode-based volume metering in digital microfluidics". Microfluidics and ... Digital microfluidics (DMF) is a platform for lab-on-a-chip systems that is based upon the manipulation of microdroplets. ...
Get Microfluidics and Nanotechnology now with OReilly online learning.. OReilly members experience live online training, plus ... Get Microfluidics and Nanotechnology now with OReilly online learning.. OReilly members experience live online training, plus ...
Microfluidics. Since blood is a moving biological fluid in vivo, it is important to study thrombosis formation in vitro under ... Microfluidics and coagulation biology. Annu Rev Biomed Eng 2013; 15 : 283-303 ... This feature of microfluidics lends itself to combinatorial studies of pro- and anti-coagulant molecules that are typically ...
2005) Microfluidics: Fluid physics at the nanoliter scale. Rev Mod Phys 77(3):977-1026. ... 2014) Mail-order microfluidics: Evaluation of stereolithography for the production of microfluidic devices. Lab Chip 14(7):1294 ... 3D-printed microfluidics. Microfluidic technology has been broadly applied in domains including biochemical analysis, ... 2014) The present and future role of microfluidics in biomedical research. Nature 507(7491):181-189. ...
Microfluidics Micromilling Low cost Microfabrication Droplet microfluidics Electronic supplementary material. The online ... Squires TM, Quake SR (2005) Microfluidics: fluid physics at the nanoliter scale. Rev Mod Phys 77(3):977CrossRefGoogle Scholar ... Tsao CW, DeVoe DL (2009) Bonding of thermoplastic polymer microfluidics. Microfluid Nanofluid 6(1):1-16CrossRefGoogle Scholar ... Whitesides GM (2006) The origins and the future of microfluidics. Nature 442(7101):368-373CrossRefGoogle Scholar ...
The demand for microfluidics is growing rapidly in pharmaceutical and biotechnology research, where they are widely used in ... Microfluidics: Whats the Big Deal?. Microfluidic devices incorporate all steps in the biological measurement process - from ... Early microfluidics were fabricated from silicon and glass using clean-room techniques and facilities already in use for ... To print channels small enough for microfluidics, researchers had to make their own 3D printer and find a resin that could do ...
Tags: Brain, Cell, Cell Culture, Cytokine, Cytokines, Drugs, Gene, Hospital, in vitro, Inflammation, Microfluidics, ... Modeling Alzheimers Disease Using Microfluidics. Dr. Joseph Park. An interview with Dr. Joseph Park, the incorporation of ...
Compartmentalized microfluidics: droplet microfluidics, digital microfluidics. *Biomedical applications: lab on a chip, ... Micro-fluidics is the science and technology of manipulating and analyzing fluid flow in sub-millimeter dimensions. It is the ... Microfluidics approaches can be used to characterize and even manufacture in a controlled way functional particles based on ... In this course, you will learn about physical principles that play an important role in micro-fluidics, how these principles ...
Microfluidics based Lab-on-a-chip technology exhibits an unprecedented perspective in studying microbiology and biochemistry. ... The combination of microfluidics and microbiology also opens a brand new field for education, specifically, bionanotechnology. ... Thus microfluidics enables quantitative measurements with high biological/chemical selectivity and sensitivity, as well as high ... With their expertise respectively in microfluidics, microfabrication, microbiology, environmental science, microelectronics and ...
Biomedical applications of microfluidics. *. Microfluidic fabrication. *. Hybrid microfluidic applications. Microfluidics has ... Biological Applications of Microfluidics details recent advances in the biological applications of microfluidics, including ... Microfluidics-todays applications and tomorrows potential. Microfluidics has facilitated major biochemical application ... 2 Using Microfluidics to Understand and Control the Cellular Microenvironment.. 2.1 Introduction: Engineering the ...
High-throughput injection with microfluidics using picoinjectors. Adam R. Abate, Tony Hung, Pascaline Mary, Jeremy J. Agresti, ... High-throughput injection with microfluidics using picoinjectors. Adam R. Abate, Tony Hung, Pascaline Mary, Jeremy J. Agresti, ... In droplet-based microfluidics, drops are used as "test tubes" for reactions (1, 2); the drops can be formed (3, 4), filled ... High-throughput injection with microfluidics using picoinjectors. Adam R. Abate, Tony Hung, Pascaline Mary, Jeremy J. Agresti, ...
2018 by CRC Press The Microfluidics and Nanofluidics Handbook: Two-Volume Set comprehensively captures the cross-disciplinary ... System Integration in Microfluidics. Morteza Ahmadi, John T.W. Yeow, and Mehdi Shahini. Fluidic Interconnects for Microfluidics ... Microfluidics and Nanofluidics Handbook Fabrication, Implementation, and Applications By Sushanta K. Mitra. , Suman Chakraborty ... Magnetic-Particle-Based Microfluidics. Ranjan Ganguly, Ashok Sinha, and Ishwar K. Puri. The Influence of Microfluidic Channel ...
The advantages of microfluidics have been clearly established in the field of analytical and bioanalytical sciences and in the ... Microfluidics has been used in materials science mainly for the improvement of nanoparticle synthesis, namely metal, metal ... Microfluidics in inorganic chemistry.. Abou-Hassan A1, Sandre O, Cabuil V. ... The application of microfluidics in chemistry has gained significant importance in the recent years. Miniaturized chemistry ...
Experience in the field of microfluidics is a bonus. Students who may be eligible for an NSERC Scholarship should get in touch ... Due to the popularity of "droplet-based microfluidics" it is important that the microfluidic community develops an in-depth ... he also worked on a project that involved testing alternating droplets formation in microfluidics devices (CHEM 298, September ...
4) Manz, A.; Fettinger, J. C.; Verpoorte, E.; Ludi, H.; Widmer, H. M.; Harrison, D. J. Trends Anal. Chem. 1991, 10, 144. (5) Harrison, D. J.; Manz, A.; Fan, Z.; Ludi, H.; Widmer, H. M. Anal. Chem. ¨ 1992, 64, 1926. (6) Khandurina, J.; Guttman, A. J. Chromatogr., A 2002, 943, 159. (7) Daykin, R. N. C.; Haswell, S. J. Anal. Chim. Acta 1995, 313, 155. (8) Doku, G. N.; Haswell, S. J. Anal. Chim. Acta 1999, 382, 1. (9) Greenway, G. M.; Haswell, S. J.; Petsul, P. H. Anal. Chim. Acta 1999, 387, 1. (10) Petsul, P. H.; Greenway, G. M.; Haswell, S. J. Anal. Chim. Acta 2001, 428, 155. (11) Hadd, A. G.; Jacobson, S. C.; Ramsey, J. M. Anal. Chem. 1999, 71, 5206. 10.1021/ac048675y CCC: $30.25 © 2005 American Chemical Society Published on Web 01/29/2005 1330 Analytical Chemistry, Vol. 77, No. 5, March 1, 2005 1 M HCl and diluting to 1000 mL with water. Q. 25.. 15. 1442. A green LED (508 nm. the exposed section was treated with dichlorodimethylsilane for 10 min to produce a hydrophobic surface that reduced ...
... 11.09.2014. A tiny device produces oscillatory flows that enhance the mixing of ...
Virtual Microfluidics. April 7, 2017. December 29, 2009. by Brian Wang A new method of moving tiny particles using magnetic ... "virtual microfluidics." This could reduce the cost and increase the flexibility of the devices, which might be used for such ...
... Lab Chip. 2008 Feb;8(2):198-220. doi: 10.1039/b715524g. Epub 2008 Jan 11. ...
Droplet-based microfluidics has emerged as one of the most active research fields in microfluidics. As the worlds leading ... Registration for Lab on a Chip International Symposium: Droplet-based Microfluidics is now open.. Please read the registration ... Lab on a Chip International Symposium: Droplet-based Microfluidics. 12 - 13 November 2016, Hangzhou, China ... This high-quality international symposium will bring together leading experts in all fields of droplet-based microfluidics from ...
... Innovative Biosensor Engineering from Wasatch Microfluidics. Wasatch is dedicated to the advancement of ...
... a combination of single-nucleus RNA sequencing and microfluidics, can enable massively parallel gene expression studies of ... with microfluidics, allowing massively parallel measurement of gene expression in structurally complicated tissues. ... the Broad team turned to microfluidics. Their inspiration: Drop-Seq, a single-cell RNA-Seq (scRNAseq) technique that ...
... s digital microfluidics unit to form its US subsidiary. ... Nuclera acquires E Ink digital microfluidics unit. Rebecca Kuo ... Instead of the physical channels found in conventional microfluidics, digital microfluidics uses electronic signals to guide ... UK-based biotech firm Nuclera has announced it has acquired Taiwan-based E Ink Holdings (EIH)s digital microfluidics unit to ... We are proud to work with E Ink to make biology accessible by printing biology with digital microfluidics. This acquisition ...
Microfluidics Novartis Creates "Inflection Point" for AbCellera with 10-Target Antibody Collaboration. ...
PRNewswire/ -- According to the new market research report Microfluidics Market by Product (Devices, Components (Chips, ... CHICAGO, May 15, 2020 /PRNewswire/ -- According to the new market research report Microfluidics Market by Product (Devices, ... The global microfluidics market is highly competitive, with a large number of global and local market players competing for ... US), Fluidigm Microfluidics (US), Abbott Laboratories (US), and QIAGEN (Netherlands) are some of the prominent players in the ...
No Small Thing: Microfluidics. Microfluidics is generally used to describe any technology that moves microscopic and nano-scale ...
Often, the microfluidics researcher worries not about how to solve relevant governing equations and boundary conditions, but ... The aim of the interdisciplinary short course is to give a practical introduction on microfluidics and the often astonishing ... Manufacturers producing equipment and measurement techniques for microfluidics will introduce their products and the exchange ... scientists and industrial researchers who have already started to work in the field of microfluidics, or plan to do that in the ...
  • Digital microfluidics (DMF) is a platform for lab-on-a-chip systems that is based upon the manipulation of microdroplets. (wikipedia.org)
  • Digital microfluidics can be used together with analytical analysis procedures such as mass spectrometry, colorimetry, electrochemical, and electrochemiluminescense. (wikipedia.org)
  • And in contrast to continuous-flow microfluidics, digital microfluidics works much the same way as traditional bench-top protocols, only with much smaller volumes and much higher automation. (wikipedia.org)
  • Digital microfluidics can be light-activated. (wikipedia.org)
  • UK-based biotech firm Nuclera has announced it has acquired Taiwan-based E Ink Holdings (EIH)'s digital microfluidics unit to form its US subsidiary. (digitimes.com)
  • Instead of the physical channels found in conventional microfluidics, digital microfluidics uses electronic signals to guide microdroplets, according to Nuclera. (digitimes.com)
  • We are proud to work with E Ink to make biology accessible by printing biology with digital microfluidics. (digitimes.com)
  • Important applications include simulations of lab-on-a-chip devices, digital microfluidics, electrokinetic and magnetokinetic devices, inkjets, and micro-pumps. (imechanica.org)
  • The meeting will cover all types of devices (lab-on-chip, digital microfluidics, paper microfluidics, total analytical systems etc.) and concern application to all forms of molecular analysis (biomedical, environmental, forensic, quality control etc. (rsc.org)
  • The demand for microfluidics is growing rapidly in pharmaceutical and biotechnology research, where they are widely used in proteomics, high-throughput DNA sequencing, gene analysis, and enzymatic assays. (asme.org)
  • In addition to these factors, the demand for microfluidics technology is increasing owing to the growing demand and adoption of PoC diagnostic devices. (prnewswire.com)
  • The Morgridge Institute Medical Engineering team, responding to increased UW-Madison demand for microfluidics resources, has launched a new microfluidics foundry to build customized tools that bring biology down to the smallest possible scale. (wisconsintechnologycouncil.com)
  • [8] To date, the most successful commercial application of microfluidics is the inkjet printhead . (wikipedia.org)
  • The application of microfluidics in chemistry has gained significant importance in the recent years. (nih.gov)
  • This is Will's second research project in the Elvira Lab, he also worked on a project that involved testing alternating droplets formation in microfluidics devices (CHEM 298, September to December 2017). (uvic.ca)
  • With their expertise respectively in microfluidics, microfabrication, microbiology, environmental science, microelectronics and surface engineering, they are confident in achieving the proposed interdisciplinary initiatives in all aspects of research, teaching and training. (uwo.ca)
  • This invention relates generally to the fields of laboratory automation, microfabrication and manipulation of small volumes of fluids (microfluidics), in such a manner so as to enable rapid dispensing and manipulation of small isolated volumes of fluids under direct electronic control. (google.com)
  • Advances in microfluidics technology are revolutionizing molecular biology procedures for enzymatic analysis (e.g., glucose and lactate assays ), DNA analysis (e.g., polymerase chain reaction and high-throughput sequencing ), and proteomics . (wikipedia.org)
  • Droplet based microfluidics show remarkable control over the experimental conditions, excellent observation capabilities and high throughput of experimental results [6]. (aiche.org)
  • Inertial microfluidics for sheath-less high-throughput flow cytometry. (cdc.gov)
  • Microfluidics deals with the behaviour, precise control and manipulation of fluids that are geometrically constrained to a small, typically sub-millimeter, scale at which capillary penetration governs mass transport. (wikipedia.org)
  • Microfluidics allows manipulation of laminar flow of fluids in channels, thereby creating concentration gradients of several orders of magnitude. (news-medical.net)
  • Microfluidics refers to the behaviour, precise control, and manipulation of fluids that are geometrically constrained to a small scale (typically sub-millimeter) at which surface forces dominate volumetric forces. (wikipedia.org)
  • This is based on microfluidics , which is the science of manipulating and controlling fluids. (digitaljournal.com)
  • Microfluidics is the behavior of fluids at the microscale level. (innovations-report.com)
  • Microfluidics studies various systems that are able to process small quantities of fluids by using tiny channels having dimensions in micrometres. (medgadget.com)
  • The Microfluidics and Nanofluidics Handbook: Two-Volume Set comprehensively captures the cross-disciplinary breadth of the fields of micro- and nanofluidics, which encompass the biological sciences, chemistry, physics and engineering applications. (routledge.com)
  • To fill the knowledge gap between engineering and the basic sciences, the editors pulled together key individuals, well known in their respective areas, to author chapters that help graduate students, scientists, and practicing engineers understand the overall area of microfluidics and nanofluidics. (routledge.com)
  • Reflecting the inherent nature of microfluidics and nanofluidics, the book includes as much interdisciplinary knowledge as possible. (routledge.com)
  • Please join us for a national workshop on Microfluidics and Nanofluidics near Delhi, India during 14-16 July 2014. (imechanica.org)
  • Ayoib A, Hashim U, Arshad MM, Thivina V (2016) Soft lithography of microfluidics channels using su-8 mould on glass substrate for low cost fabrication. (springer.com)
  • 2016) show how coupling microfluidics capillary electrophoresis with mass spectrometric analysis is a useful tool for characterizing intact monoclonal antibody-drug conjugates (ADCs) and for monitoring glycated hemoglobin in diabetes management. (thermofisher.com)
  • In paper based microfluidics, capillary elements can be achieved through the simple variation of section geometry. (wikipedia.org)
  • This section also provides the overall information and data analysis of the global microfluidics market with respect to the leading market segments based on major products, applications, key materials utilized, end-users, and geographies. (openpr.com)
  • The global microfluidics market has been studied based on major product segments, application segments, material segments, their end-users, and regional as well as national markets. (openpr.com)
  • The market overview section of the report explores the market dynamics such as drivers, restraints, and opportunities that currently have a strong impact on the global microfluidics market and could influence the market in the near future. (openpr.com)
  • The "Global Microfluidics Market Analysis to 2025" is a specialized and in-depth study of the microfluidics industry with a focus on the global market trend. (medgadget.com)
  • The report aims to provide an overview of global microfluidics market with detailed market segmentation by product, material, application, and geography. (medgadget.com)
  • The global microfluidics market is expected to witness high growth during the forecast period. (medgadget.com)
  • It provides overview and forecast of the global microfluidics market based on product, material and application. (medgadget.com)
  • By giving researchers easier, more affordable access to microdevices in the same size regime as complex commercially made devices, 3D printing could propel microfluidics to new heights in mainstream biological research and clinical diagnostics. (asme.org)
  • To print channels small enough for microfluidics, researchers had to make their own 3D printer and find a resin that could do it. (asme.org)
  • Manufacturers producing equipment and measurement techniques for microfluidics will introduce their products and the exchange with experienced researchers and other participants will widen the scientific horizon. (tu-ilmenau.de)
  • The course is intended for PhD students, scientists and industrial researchers who have already started to work in the field of microfluidics, or plan to do that in the near future and want to get familiar with the diverse possibilities of microfluidics. (tu-ilmenau.de)
  • I can say that the book will interest many researchers all around the world … This type of books is really necessary for researchers, academics and even microfluidics-implicated companies! (routledge.com)
  • Researchers develop a method that combines the strengths of shotgun metagenomics and single-cell genome sequencing in a microfluidics-based platform. (the-scientist.com)
  • New advances for the detection of cancer led by Rafael V. Davalos of the Virginia Tech-Wake Forest School of Biomedical Engineering and Science (SBES) are featured as the cover story in the January 19, 2010 Royal Society of Chemistry's magazine, "Lab on a Chip," the premier journal for researchers in microfluidics. (innovations-report.com)
  • This conference is held concurrently with the SelectBIO Lab-on-a-Chip and Microfluidics World Congress 2019 which now in its 11th year is an Established Annual Event attracting researchers from academia and industry from around the world. (selectbiosciences.com)
  • It is also directed towards researchers in Microfluidics, Micro/Nanofabrication and Biophyics. (eurobuch.com)
  • The EMBL Microfluidics Conference 2018 aims to bring together top researchers in the field and to spark scientific exchange, also across different disciplines. (rsc.org)
  • As a practical demonstration of the advantages of graphene-based microfluidics, the researchers used their device to determine the crystal structure of hen egg white lysozyme (HEWL). (nanowerk.com)
  • Despite advantages in reduced sample-handling and preparation times alongside an attendant decrease in error, researchers may not be familiar with the role that microfluidics could play in mass spectrometry-based analytical workflows. (thermofisher.com)
  • The second paper describes how the microfluidics platform also achieves in-line separation compatible with ESI-MS, allowing researchers to determine circulating glycosylated HbA1c hemoglobin levels as an indication of glycemic control in diabetes. (thermofisher.com)
  • The researchers expect that the electrogates can be easily implemented into low-power, portable microfluidics devices in the future. (phys.org)
  • The researchers hope that the pump could make microfluidics-based healthcare applications, such as point-of-care diagnostics, more accessible around the world. (medgadget.com)
  • aims to bring together leading academic scientists, researchers and research scholars to exchange and share their experiences and research results on all aspects of Microfluidics Actuators and 3D Printing. (waset.org)
  • Get Microfluidics and Nanotechnology now with O'Reilly online learning. (oreilly.com)
  • With an intense focus on micro- and nanotechnology from a fluidic perspective, Microfluidics and Microscale Transport Processes provides a broad review of advances in this field. (routledge.com)
  • Microfluidics Promotes Scientific University Research Using Nanotechnology with Innovation I... ( New Academic. (bio-medicine.org)
  • Newton, MA (PRWEB) May 17, 2010 -- Microfluidics (OTCBB: MFLU), the exclusive global provider of Microfluidizer(R) high shear fluid processors, has initiated a formal collaboration program to further promote the use of nanotechnology in scientific research at the university level. (bio-medicine.org)
  • Partnering with Microfluidics has enabled us to advance exciting new nanotechnology-based methods for drug delivery, for example, to penetrate the blood brain barrier," said Mansoor Amiji, Ph.D., Distinguished Professor and Chair of Pharmaceutical Sciences at Northeastern University. (bio-medicine.org)
  • Microfluidics has been used to devise systems capable of generating miniscule liquid droplets in the microchannels. (news-medical.net)
  • Droplets in microfluidics have been used as micro-reactors [7] and as vessels to study cell-cell interactions [8]. (aiche.org)
  • Microfluidics can be used to produce emulsions but also to study the stability of emulsion droplets during production and further processing [1]. (wur.nl)
  • As a broad generalization, clinicians and laboratory personnel who use microfluidics-based automated or semi-automated instrumentation to perform biomedical assays on real-world samples are more pleased with the state of the assays than they are with the state of the front-end sample preparation. (springer.com)
  • The advantages of microfluidics have been clearly established in the field of analytical and bioanalytical sciences and in the field of organic synthesis. (nih.gov)
  • Often, the microfluidics researcher worries not about how to solve relevant governing equations and boundary conditions, but rather what those equations and boundary conditions are and how his or her analytical goals can be reconciled with fabrication and experimentation concerns. (tu-ilmenau.de)
  • 1st UK Microfluidics for Analytical Chemistry Conference will be held on Thursday 1 February 2018 at the National Oceanography Centre in Southampton , UK . (rsc.org)
  • Here, we introduce the picoinjector, a robust device to add controlled volumes of reagent using electro-microfluidics at kilohertz rates. (pnas.org)
  • It can be a worthy challenge to take a raw sample, introduce it into a microfluidics-based system, and perform the sample preparation, which may include separation and concentration of the target analytes, so that one can benefit from the reagent-conserving small volumes and obtain the correct answer when finally implementing the assay of interest. (springer.com)
  • Microfluidics can be used to increase performance, reduce reagent consumption and increase throughput in chemical analysis. (dissertations.se)
  • Thus microfluidics enables quantitative measurements with high biological/chemical selectivity and sensitivity, as well as high temporal and spatial resolution. (uwo.ca)
  • Biological Applications of Microfluidics details recent advances in the biological applications of microfluidics, including cell sorting, DNA sequencing on a chip, microchip capillary electrophoresis, and synthesis on a microfluidic format. (wiley.com)
  • The chapters discuss a wide range of issues in the sub-domains of capillary transport, fluidic resistance, electrokinetics, substrate modification, rotational microfluidics, and the applications of the phenomena of these sub-domains in diverse situations ranging from non-biological to biological ones like DNA hybridization and cellular biomicrofluidics. (routledge.com)
  • the integrated microfluidics platform provides in-line separation by capillary electrophoresis within the chip itself. (thermofisher.com)
  • Here, we discuss recent advances in the use of microfluidics to separate trypanosomes from blood and to isolate single trypanosomes for analyses including drug screening. (mdpi.com)
  • Microfluidics has facilitated major biochemical application advancements in point-of-care diagnostics, bioterrorism detection, and drug discovery. (wiley.com)
  • Based on applications, the microfluidics market is classified broadly into three major application segments, i.e., in-vitro diagnostics, pharmaceutical and life science research and manufacturing, and therapeutics. (prnewswire.com)
  • On the basis of applications the microfluidics market is classified as peripheral vascular diseases, Wound care management, pharmaceutical & biotech Research, Invitro Diagnostics (IVD), Drug Delivery and Others. (openpr.com)
  • NEW YORK (GenomeWeb News) - GenMark Diagnostics today said it has been granted a non-exclusive license to PerkinElmer's microfluidics patent portfolio. (genomeweb.com)
  • Although microfluidics devices have a wide variety of uses, from point-of-care diagnostics to environmental analysis, one major limitation is that they cannot be modified for different uses on the fly, since their flow paths are set during fabrication. (phys.org)
  • Thus, to realize the full potential of droplet-based microfluidics, a robust technique for adding reagents is needed. (pnas.org)
  • Droplet-based microfluidics has emerged as one of the most active research fields in microfluidics. (rsc.org)
  • This high-quality international symposium will bring together leading experts in all fields of droplet-based microfluidics from all around the world. (rsc.org)
  • The NNIN/C at the University of Michigan will be hosting a presentation on " Multiphase CFD for Droplet Based Microfluidics", which will be broadcast live as a web based seminar. (imechanica.org)
  • In short, software rather than hardware could control the chip's properties, allowing it to be instantly reconfigured through changes in the controlling software - an approach Alexander-Katz refers to as "virtual microfluidics. (nextbigfuture.com)
  • NEW YORK (GenomeWeb) - Scientists at MIT and the Broad Institute have developed an inexpensive "virtual microfluidics" method to compartmentalize samples for amplification reactions that could be applied to single-cell sequencing and digital PCR. (genomeweb.com)
  • Ultimately, we clarified the suitability of low-cost micromills and a cost-efficient assembly method in the field of microfluidics by demonstrating rate- and size-controlled microfluidic droplet generation. (springer.com)
  • Microfluidics technology has contributed to powerful tools that have helped advance many areas of biology. (news-medical.net)
  • Beebe DJ, Mensing GA, Walker GM (2002) Physics and applications of microfluidics in biology. (springer.com)
  • Automating microfluidics for synthetic biology to advance areas in energy and health. (concordia.ca)
  • Microfluidics in Cell Biology Part B: Microfluidics in Single Cells, Volume 147, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. (elsevier.com)
  • Microfluidics is a quickly growing, highly interdisciplinary field at the interface of physics, engineering, chemistry and biology. (rsc.org)
  • Microfluidics emerged in the beginning of the 1980s and is used in the development of inkjet printheads, DNA chips , lab-on-a-chip technology, micro-propulsion, and micro-thermal technologies. (wikipedia.org)
  • Microfluidics based Lab-on-a-chip technology exhibits an unprecedented perspective in studying microbiology and biochemistry. (uwo.ca)
  • In this paper we describe a sheath-less, on-chip flow cytometry system based on the principle of Dean coupled inertial microfluidics. (cdc.gov)
  • On-chip characterization Microfluidics in the classroom On-chip acid-base titrations. (primidi.com)
  • A team of experimenters from the University of Massachusetts Amherst and The University of Chicago utilized high-brightness x-rays from the U.S. Department of Energy s Advanced Photon Source (APS) at Argonne as they developed a new strategy to combat these limitations by combining microfluidics with another cutting-edge technology, single-layer graphene, for serial protein crystallography in an ultrathin microfluidic chip. (nanowerk.com)
  • Their work was published in Lab On a Chip ( 'Graphene-based microfluidics for serial crystallography' ). (nanowerk.com)
  • Active microfluidics refers to the defined manipulation of the working fluid by active (micro) components such as micropumps or microvalves . (wikipedia.org)
  • Microfluidics approaches can be used to characterize and even manufacture in a controlled way functional particles based on soft materials. (tue.nl)
  • In addition, microfluidics-based devices, capable of continuous sampling and real-time testing of air/water samples for biochemical toxins and other dangerous pathogens , can serve as an always-on "bio-smoke alarm" for early warning. (wikipedia.org)
  • However, some aspects of microfluidics such as the ease of use, materials used in the production of microfluidic devices, and integration with other systems need addressing so that it can be more widely applied to complex stem cell research problems. (news-medical.net)
  • In addition, open microfluidics eliminates the need to glue or bond a cover for devices, which could be detrimental to capillary flows. (wikipedia.org)
  • Students can apply the theory of microfluidics to guide the design of microfluidic devices and to interpret experimental results. (tue.nl)
  • Based on products, the microfluidics market is segmented into microfluidic-based devices and microfluidic components. (prnewswire.com)
  • In this Workshop, we will focus on COMSOL's Microfluidics Module which encompasses easy-to-use tools for the study of microfluidic devices. (imechanica.org)
  • I have just started working on ELISA on microfluidics devices. (protocol-online.org)
  • The Advanced Fabrication Laboratory has a suite of new machines that can mold, emboss and etch polymer micro-channels and create the precise surface properties required in microfluidics devices. (wisconsintechnologycouncil.com)
  • They may be more familiar with reports of coupling problems between microfluidics devices and MS instrumentation that hinder efficient use. (thermofisher.com)
  • The Microfluidics Module brings you easily-operated tools for studying microfluidic devices. (comsol.se)
  • A system of Photoactuated Droplet Microfluidics (PDM), in which a droplet of fluid is moved by way of the manipulation of the wettability of a spiropyran functionalized surface via irradiation with ultraviolet and visible light, was proposed. (oregonstate.edu)
  • Microfluidics in inorganic chemistry. (nih.gov)
  • Running alongside will be a co-located exhibit hall composed of companies from around the world developing tools, technologies and services in the microfluidics and flow chemistry fields. (selectbiosciences.com)
  • This 5th biennial GRC conference on the Physics & Chemistry of Microfluidics will take place from June 28th - July 3rd at the Il Ciocco Hotel & Resort in Lucca (Barga), Italy. (grc.org)
  • Another advantage of open microfluidics is the ability to integrate open systems with surface-tension driven fluid flow, which eliminates the need for external pumping methods such as peristaltic or syringe pumps. (wikipedia.org)
  • Continuous flow microfluidics rely on the control of a steady state liquid flow through narrow channels or porous media predominantly by accelerating or hindering fluid flow in capillary elements. (wikipedia.org)
  • The aim of the interdisciplinary short course is to give a practical introduction on microfluidics and the often astonishing effects that make the flow physics in many situations completely different from the macroscopic world. (tu-ilmenau.de)
  • The theoretical introduction is complemented by practical sessions in which the participants can get first hands on experiences on the flow field measurements techniques, will built their own chips with soft lithography, learn about data processing and get the possibility to perform laboratory work in the field of microfluidics. (tu-ilmenau.de)
  • role in microfluidics, e.g., flow control in microchannels. (scribd.com)
  • Delamarche, E. Advanced Capillary Soft Valves for Flow Control in Self-Driven Microfluidics. (mdpi.com)
  • Electrogates for stop-and-go control of liquid flow in microfluidics. (phys.org)
  • Ultra-rapid flow-through polymerase chain reaction microfluidics using vapor pressure. (biomedsearch.com)
  • The Microfluidics Module includes ready-to-use user interfaces and simulation tools, so called physics interfaces, for single-phase flow, porous media flow, two-phase flow, and transport phenomena. (comsol.se)
  • The Microfluidics Module is designed specifically for handling momentum, heat, and mass transport with special considerations for fluid flow at the microscale. (comsol.se)
  • As an increasingly commonplace laboratory technology, 3D printing is eyed as the key to bringing microfluidics into the mainstream. (asme.org)
  • Students have an overview of different technological and scientific applications of microfluidics technology. (tue.nl)
  • Microfluidics is generally used to describe any technology that moves microscopic and nano-scale quantities of fluid through channels on a MEMS (Micro-Electro-Mechanical System). (coleparmer.com)
  • Leveraging microfluidics technology, the LabChip GXII Touch HT eliminates the need to handle time consuming SDS page gels, and provides superior results for less time and money. (perkinelmer.com)
  • Microfluidics provides an enabling technology which has allowed us to develop novel properties for pharmaceutical, nutraceutical and cosmeceutical applications. (bio-medicine.org)
  • Continuous manufacturing via crystallization and chemical reactions using bottom-up Microfluidics Reaction Technology(TM) (MRT), for example, was developed in a partnership between Microfluidics CTO Thomai "Mimi" Panagiotou, Ph.D., and Robert Fisher, Ph.D., of the Chemical Engineering Department at the Massachusetts Institute of Technology. (bio-medicine.org)
  • in their review and in the recent press release, coupling this new microfluidics technology to MS instrumentation is becoming more straightforward. (thermofisher.com)
  • Additionally, the market exhibits growth opportunities due growing applications of microfluidics technology. (medgadget.com)
  • Also, high quality research contributions describing original and unpublished results of conceptual, constructive, empirical, experimental, or theoretical work in all areas of Microfluidics Actuators and 3D Printing are cordially invited for presentation at the conference. (waset.org)
  • ICMAP 2020 has teamed up with the Special Journal Issue on Microfluidics Actuators and 3D Printing . (waset.org)
  • The stakeholders of this report include companies and intermediaries engaged in the manufacture and commercialization of various microfluidics instrument and products as well as new entrants planning to enter this market. (openpr.com)
  • The LabChip ® GX Touch microfluidics platforms perform DNA and RNA separation and analysis using automated micro-ce with minimal concentration requirements. (perkinelmer.com)
  • The DropletQuant complements PerkinElmerlj existing LabChip microfluidics instrument portfolio, which provides electrophoretic separation and analysis of nucleic acids, proteins and glycan profiling. (perkinelmer.com)
  • 5 Engineering Cellular Microenvironments with Microfluidics. (wiley.com)
  • Microfluidics has revolutionized the way we study the motile behavior of cells by enabling observations at high spatial and temporal resolution in carefully controlled microenvironments. (mit.edu)
  • Fluidigm microfluidics technologies have transformed the time and workload of these single-cell experiments with automation and conservation of sample and reagents. (fluidigm.com)
  • On the basis of product, the microfluidics market is segmented into microfluidic chips, microfluidic sensors, micropumps, microneedles and others. (medgadget.com)
  • Going forward, microfluidics will hopefully play a greater role in developing economical methods for single-cell assays, cell-based assays, gene-expression profiling, and phenotypic screenings that can transform health care research globally. (news-medical.net)
  • Journal Club' presentations of recent microfluidics research articles. (tue.nl)
  • Based on end-users, the microfluidics market is segmented into hospitals and diagnostic centers, academic and research institutes, and pharmaceutical and biotechnology companies. (prnewswire.com)
  • The importance of academic research to the pharmaceutical industry's drug development strategy cannot be understated, and it's increasing every day," said Michael C. Ferrara, Microfluidics President and CEO. (bio-medicine.org)
  • Additionally, microfluidics is also used in diagnosis of diseases such as cancer and in research. (medgadget.com)
  • Said Dr. Fisher, "I was intrigued by the opportunity to work closely with Microfluidics to develop improved drug fabrication techniques that enhance delivery and efficacy, which pharmaceutical firms will find extremely compelling. (bio-medicine.org)
  • With numerous advantages such as the requirement of small volumes, reduced costs, and lesser waste generation, microfluidics makes several new investigations possible for biologists and biochemists. (news-medical.net)
  • Advantages of open microfluidics include accessibility to the flowing liquid for intervention, larger liquid-gas surface area, and minimized bubble formation. (wikipedia.org)
  • The Polymers and Microfluidics group is centred on experimental soft condensed matter. (imperial.ac.uk)
  • So how can microfluidics aid mass spectrometric detection and analysis? (thermofisher.com)
  • Fluid Mechanics for Chemical Engineers, Second Edition, with Microfluidics and CFD, systematically introduces fluid mechanics from the perspective of the chemical engineer who must understand actual physical behavior and solve real-world problems. (informit.com)
  • This feature of microfluidics lends itself to combinatorial studies of pro- and anti-coagulant molecules that are typically done in well-plate assays under static conditions. (upenn.edu)
  • Synthetic biologists have used microfluidics for DNA assembly, cell-free expression, and cell culture, but a combination of expense, device complexity, and reliance on custom set-ups hampers their widespread adoption. (mit.edu)
  • Microfluidics provides the opportunity to study cells effectively on both a single- and multi-cellular level with high-resolution and localized application of experimental conditions with biomimetic physiological conditions. (rsc.org)
  • Compartmentalization maintains the genotype-phenotype link, making droplet microfluidics suitable for screening of extracellular traits such as secreted products and for screening of microcolonies originating from single cells. (kth.se)
  • In recent years, microfluidics has demonstrated novel methods of culturing and studying cells. (nsti.org)
  • Unique to this updated volume are three sections on microfluidics in various single cell models, including microfludics in micro-organisms, microfluidics for cell culture and cell sorting of mammalian cells, and microfluidics for cell migration. (elsevier.com)
  • It took one post-doctoral fellow five years to sequence 250 cells from diabetic mice-work that now represents two experiments and two weeks of work, with efficiency of cell recovery reaching 60-70%, thanks to microfluidics. (fluidigm.com)
  • The appeal of microfluidics for this procedure is partly based on its combination of small size and its ability to process very small liquid volumes, thus minimizing the use of possibly expensive reagents. (springer.com)
  • Engineers and chemists from Brigham Young University, Provo, UT, have broken through a major barrier to the wider use of 3D-printed microfluidics. (asme.org)
  • Fluid Mechanics for Chemical Engineers, Second Edition, with Microfluidics and CFD, includes 83 completely worked practical examples, several of which involve FlowLab and COMSOL Multiphysics. (informit.com)
  • Students are able to understand and summarize scientific papers at the forefront of the microfluidics field. (tue.nl)
  • The combination of microfluidics and microbiology also opens a brand new field for education, specifically, bionanotechnology. (uwo.ca)
  • Existing construction processes for microfluidics use complex, cumbersome, and expensive lithography methods that produce single-material, multi-layered 2D chips. (mit.edu)
  • Multi-material 3D printing presents a promising alternative to existing methods that would allow microfluidics to be fabricated in a single step with functionally graded material properties. (mit.edu)