Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.
The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES.
Silicone polymers which consist of silicon atoms substituted with methyl groups and linked by oxygen atoms. They comprise a series of biocompatible materials used as liquids, gels or solids; as film for artificial membranes, gels for implants, and liquids for drug vehicles; and as antifoaming agents.
The largest country in North America, comprising 10 provinces and three territories. Its capital is Ottawa.
Methodologies used for the isolation, identification, detection, and quantitation of chemical substances.
Microdevices that combine microfluidics technology with electrical and/or mechanical functions for analyzing very small fluid volumes. They consist of microchannels etched into substrates made of silicon, glass, or polymer using processes similar to photolithography. The test fluids in the channels can then interact with different elements such as electrodes, photodetectors, chemical sensors, pumps, and valves.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of PROTEINS; NUCLEIC ACIDS; and CARBOHYDRATES. (Segen, Dictionary of Modern Medicine, 1992)
Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal.
Methods of creating machines and devices.
The preparation and analysis of samples on miniaturized devices.
A highly miniaturized version of ELECTROPHORESIS performed in a microfluidic device.
The systematic identification and quantitation of all the metabolic products of a cell, tissue, organ, or organism under varying conditions. The METABOLOME of a cell or organism is a dynamic collection of metabolites which represent its net response to current conditions.
The spectrometric analysis of fluorescent X-RAYS, i.e. X-rays emitted after bombarding matter with high energy particles such as PROTONS; ELECTRONS; or higher energy X-rays. Identification of ELEMENTS by this technique is based on the specific type of X-rays that are emitted which are characteristic of the specific elements in the material being analyzed. The characteristic X-rays are distinguished and/or quantified by either wavelength dispersive or energy dispersive methods.
Antibodies that are chemically bound to a substrate material which renders their location fixed.
Manufacturing technology for making microscopic devices in the micrometer range (typically 1-100 micrometers), such as integrated circuits or MEMS. The process usually involves replication and parallel fabrication of hundreds or millions of identical structures using various thin film deposition techniques and carried out in environmentally-controlled clean rooms.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
The design or construction of objects greatly reduced in scale.
A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.
Measurement and evaluation of the components of substances to be taken as FOOD.
The monitoring of the level of toxins, chemical pollutants, microbial contaminants, or other harmful substances in the environment (soil, air, and water), workplace, or in the bodies of people and animals present in that environment.
Determination, by measurement or comparison with a standard, of the correct value of each scale reading on a meter or other measuring instrument; or determination of the settings of a control device that correspond to particular values of voltage, current, frequency or other output.
Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.
Assaying the products of or monitoring various biochemical processes and reactions in an individual cell.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
The evaluation of incidents involving the loss of function of a device. These evaluations are used for a variety of purposes such as to determine the failure rates, the causes of failures, costs of failures, and the reliability and maintainability of devices.
The motion of fluids, especially noncompressible liquids, under the influence of internal and external forces.
The analysis of a chemical substance by inserting a sample into a carrier stream of reagent using a sample injection valve that propels the sample downstream where mixing occurs in a coiled tube, then passes into a flow-through detector and a recorder or other data handling device.
A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry.
A systematic collection of factual data pertaining to health and disease in a human population within a given geographic area.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
A broad family of synthetic organosiloxane polymers containing a repeating silicon-oxygen backbone with organic side groups attached via carbon-silicon bonds. Depending on their structure, they are classified as liquids, gels, and elastomers. (From Merck Index, 12th ed)
Spectrophotometric techniques by which the absorption or emmision spectra of radiation from atoms are produced and analyzed.
The utilization of an electrical current to measure, analyze, or alter chemicals or chemical reactions in solution, cells, or tissues.
Elements of limited time intervals, contributing to particular results or situations.
Drugs intended for human or veterinary use, presented in their finished dosage form. Included here are materials used in the preparation and/or formulation of the finished dosage form.
Polymerized methyl methacrylate monomers which are used as sheets, moulding, extrusion powders, surface coating resins, emulsion polymers, fibers, inks, and films (From International Labor Organization, 1983). This material is also used in tooth implants, bone cements, and hard corneal contact lenses.
Methods for maintaining or growing CELLS in vitro.
Chromatographic techniques in which the mobile phase is a liquid.
The development and use of techniques to study physical phenomena and construct structures in the nanoscale size range or smaller.
A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained.
Alicyclic hydrocarbons in which three or more of the carbon atoms in each molecule are united in a ring structure and each of the ring carbon atoms is joined to two hydrogen atoms or alkyl groups. The simplest members are cyclopropane (C3H6), cyclobutane (C4H8), cyclohexane (C6H12), and derivatives of these such as methylcyclohexane (C6H11CH3). (From Sax, et al., Hawley's Condensed Chemical Dictionary, 11th ed)
Application of statistical procedures to analyze specific observed or assumed facts from a particular study.
Studies in which variables relating to an individual or group of individuals are assessed over a period of time.
The motion of a liquid through a membrane (or plug or capillary) consequent upon the application of an electric field across the membrane. (Oxford Dictionary of Biochemistry and Molecular Biology, 2001)
Statistical formulations or analyses which, when applied to data and found to fit the data, are then used to verify the assumptions and parameters used in the analysis. Examples of statistical models are the linear model, binomial model, polynomial model, two-parameter model, etc.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
The development and use of techniques and equipment to study or perform chemical reactions, with small quantities of materials, frequently less than a milligram or a milliliter.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
Statistical models which describe the relationship between a qualitative dependent variable (that is, one which can take only certain discrete values, such as the presence or absence of a disease) and an independent variable. A common application is in epidemiology for estimating an individual's risk (probability of a disease) as a function of a given risk factor.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Water-soluble low-molecular-weight polymers of acrylic or methacrylic acid that form solid, insoluble products when mixed with specially prepared ZnO powder. The resulting cement adheres to dental enamel and is also used as a luting agent.
Hard, amorphous, brittle, inorganic, usually transparent, polymerous silicate of basic oxides, usually potassium or sodium. It is used in the form of hard sheets, vessels, tubing, fibers, ceramics, beads, etc.
A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy.
A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both.
A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.
A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.
A plan for collecting and utilizing data so that desired information can be obtained with sufficient precision or so that an hypothesis can be tested properly.
Reducing the SURFACE TENSION at a liquid/solid interface by the application of an electric current across the interface thereby enhancing the WETTABILITY of the surface.
Characteristics or attributes of the outer boundaries of objects, including molecules.
A trace element that constitutes about 27.6% of the earth's crust in the form of SILICON DIOXIDE. It does not occur free in nature. Silicon has the atomic symbol Si, atomic number 14, and atomic weight [28.084; 28.086].
A phenomenon in which the surface of a liquid where it contacts a solid is elevated or depressed, because of the relative attraction of the molecules of the liquid for each other and for those of the solid. (from McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
Laboratory and other services provided to patients at the bedside. These include diagnostic and laboratory testing using automated information entry.
The integration of epidemiologic, sociological, economic, and other analytic sciences in the study of health services. Health services research is usually concerned with relationships between need, demand, supply, use, and outcome of health services. The aim of the research is evaluation, particularly in terms of structure, process, output, and outcome. (From Last, Dictionary of Epidemiology, 2d ed)
The frequency of different ages or age groups in a given population. The distribution may refer to either how many or what proportion of the group. The population is usually patients with a specific disease but the concept is not restricted to humans and is not restricted to medicine.
Use of various chemical separation and extraction methods, such as SOLID PHASE EXTRACTION; CHROMATOGRAPHY; and SUPERCRITICAL FLUID EXTRACTION; to prepare samples for analytical measurement of components.
The fundamental, structural, and functional units or subunits of living organisms. They are composed of CYTOPLASM containing various ORGANELLES and a CELL MEMBRANE boundary.
A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
Polymerized forms of styrene used as a biocompatible material, especially in dentistry. They are thermoplastic and are used as insulators, for injection molding and casting, as sheets, plates, rods, rigid forms and beads.
Electric conductors through which electric currents enter or leave a medium, whether it be an electrolytic solution, solid, molten mass, gas, or vacuum.
Concentration or quantity that is derived from the smallest measure that can be detected with reasonable certainty for a given analytical procedure.
Studies in which the presence or absence of disease or other health-related variables are determined in each member of the study population or in a representative sample at one particular time. This contrasts with LONGITUDINAL STUDIES which are followed over a period of time.

Metabolite fingerprinting: detecting biological features by independent component analysis. (1/1368)

MOTIVATION: Metabolite fingerprinting is a technology for providing information from spectra of total compositions of metabolites. Here, spectra acquisitions by microchip-based nanoflow-direct-infusion QTOF mass spectrometry, a simple and high throughput technique, is tested for its informative power. As a simple test case we are using Arabidopsis thaliana crosses. The question is how metabolite fingerprinting reflects the biological background. In many applications the classical principal component analysis (PCA) is used for detecting relevant information. Here a modern alternative is introduced-the independent component analysis (ICA). Due to its independence condition, ICA is more suitable for our questions than PCA. However, ICA has not been developed for a small number of high-dimensional samples, therefore a strategy is needed to overcome this limitation. RESULTS: To apply ICA successfully it is essential first to reduce the high dimension of the dataset, by using PCA. The number of principal components determines the quality of ICA significantly, therefore we propose a criterion for estimating the optimal dimension automatically. The kurtosis measure is used to order the extracted components to our interest. Applied to our A. thaliana data, ICA detects three relevant factors, two biological and one technical, and clearly outperforms the PCA.  (+info)

Microviscoelasticity of the apical cell surface of human umbilical vein endothelial cells (HUVEC) within confluent monolayers. (2/1368)

We studied the local viscoelasticity of the apical membrane of human umbilical vein endothelial cells within confluent layers by magnetic tweezers microrheometry. Magnetic beads are coupled to various integrins by coating with fibronectin or invasin. By analyzing the deflection of beads evoked by various force scenarios we demonstrate that the cell envelope behaves as a linear viscoelastic body if forces up to 2 nN are applied for short times (<20 s) but can respond in an adaptive way if stress pulses are applied longer (>30 s). The time-dependent shear relaxation modulus G(t) exhibits three time regimes: a fast response (t < 0.05 s) where the relaxation modulus G(t) obeys a power law G(t) approximately t(-0.82+/-0.02); a plateau-like behavior (at 0.05 s < t < 0.15 s); and a slow flow-like response which is, however, partially reversible. Strain field mapping experiments with colloidal probes show that local forces induce a strain field exhibiting a range of zeta = 10 +/- 1 microm, but which could only be observed if nonmagnetic beads were coupled to the cell surface by invasin. By application of the theory of elasticity of planar bodies we estimated a surface shear modulus of 2.5 x10(-4) N/m. By assuming a thickness of the actin cortex of approximately 0.5 microm we estimate a Young modulus micro approximately 400 Pa for the apical membrane. The value agrees with a plateau modulus of an entangled or weakly cross-linked actin network of an actin concentration of 100 microM (mesh size 0.2 microm). This result together with our observation of a strong reduction of the shear modulus by the actin destabilizing agent latrunculin A suggests that the shear modulus measured by our technique is determined by the actin cortex. The effect of two ligands inducing actin stress fiber formation and centripetal contraction of cells (associated with the formation of gaps in the confluent cell monolayer) on the viscoelastic responses were studied: histamine and lysophosphatidic acid (LPA). Histamine evoked a dramatic increase of the cell stiffness by >1 order of magnitude within <30 s, which is attributed to a transient rise of the intracellular Ca(2+) level, since DMSO exerted a similar effect. The stiffening is accompanied by a concomitant rounding of the cells as observed by microinterferometry and relaxes partially in the timescale of 5 min, whereas gaps between cells close after approximately 30 min. LPA did not exert a remarkable and reproducible effect other than an occasional very weak transient increase of the shear stiffness, which shows that the gap formation activated by LPA is mediated by a different mechanism than that induced by histamine.  (+info)

Formation of droplets of alternating composition in microfluidic channels and applications to indexing of concentrations in droplet-based assays. (3/1368)

For screening the conditions for a reaction by using droplets (or plugs) as microreactors, the composition of the droplets must be indexed. Indexing here refers to measuring the concentration of a solute by addition of a marker, either internal or external. Indexing may be performed by forming droplet pairs, where in each pair the first droplet is used to conduct the reaction, and the second droplet is used to index the composition of the first droplet. This paper characterizes a method for creating droplet pairs by generating alternating droplets, of two sets of aqueous solutions in a flow of immiscible carrier fluid within PDMS and glass microfluidic channels. The paper also demonstrates that the technique can be used to index the composition of the droplets, and this application is illustrated by screening conditions of protein crystallization. The fluid properties required to form the steady flow of the alternating droplets in a microchannel were characterized as a function of the capillary number Ca and water fraction. Four regimes were observed. At the lowest values of Ca, the droplets of the two streams coalesced; at intermediate values of Ca the alternating droplets formed reliably. At even higher values of Ca, shear forces dominated and caused formation of droplets that were smaller than the cross-sectional dimension of the channel; at the highest values of Ca, coflowing laminar streams of the two immiscible fluids formed. In addition to screening of protein crystallization conditions, understanding of the fluid flow in this system may extend this indexing approach to other chemical and biological assays performed on a microfluidic chip.  (+info)

Computerized microfluidic cell culture using elastomeric channels and Braille displays. (4/1368)

Computer-controlled microfluidics would advance many types of cellular assays and microscale tissue engineering studies wherever spatiotemporal changes in fluidics need to be defined. However, this goal has been elusive because of the limited availability of integrated, programmable pumps and valves. This paper demonstrates how a refreshable Braille display, with its grid of 320 vertically moving pins, can power integrated pumps and valves through localized deformations of channel networks within elastic silicone rubber. The resulting computerized fluidic control is able to switch among: (i) rapid and efficient mixing between streams, (ii) multiple laminar flows with minimal mixing between streams, and (iii) segmented plug-flow of immiscible fluids within the same channel architecture. The same control method is used to precisely seed cells, compartmentalize them into distinct subpopulations through channel reconfiguration, and culture each cell subpopulation for up to 3 weeks under perfusion. These reliable microscale cell cultures showed gradients of cellular behavior from C2C12 myoblasts along channel lengths, as well as differences in cell density of undifferentiated myoblasts and differentiation patterns, both programmable through different flow rates of serum-containing media. This technology will allow future microscale tissue or cell studies to be more accessible, especially for high-throughput, complex, and long-term experiments. The microfluidic actuation method described is versatile and computer programmable, yet simple, well packaged, and portable enough for personal use.  (+info)

Syntheses of 11C- and 18F-labeled carboxylic esters within a hydrodynamically-driven micro-reactor. (5/1368)

Carboxylic esters were successfully labeled with one of two short-lived positron-emitters, carbon-11 or fluorine-18, within a hydrodynamically-driven micro-reactor. The non-radioactive methyl ester was obtained at room temperature; its yield increased with higher substrate concentration and with reduced infusion rate. Radioactive methyl ester was obtained from the reaction of (10 mM) with in 56% decay-corrected radiochemical yield (RCY) at an infusion rate of 10 microL min(-1), and when the infusion rate was reduced to 1 microL min(-1), the RCY increased to 88%. The synthesis of the non-radioactive fluoroethyl ester from and required heating of the micro-reactor on a heating block at 80 degrees C (14-17% RCY), whilst the corresponding radioactive from and was obtained in 10% RCY. The radioactive 'peripheral' benzodiazepine receptor ligand was obtained from the reaction of acid with labeling agent in 45% RCY at an infusion rate of 10 microL min(-1). When the infusion rate was reduced to 1 microL min(-1), the RCY increased to 65%. The results exemplify a new methodology for producing radiotracers for imaging with positron emission tomography that has many potential advantages, including a requirement for small quantities of substrates, enhanced reaction, rapid reaction optimisation and easy product purification.  (+info)

Wetting morphologies at microstructured surfaces. (6/1368)

The wetting of microstructured surfaces is studied both experimentally and theoretically. Even relatively simple surface topographies such as grooves with rectangular cross section exhibit a large variety of different wetting morphologies as observed by atomic force microscopy. This polymorphism arises from liquid wedge formation along the groove corners and from contact line pinning along the groove edges. A global morphology diagram is derived that depends only on two system parameters: (i) the aspect ratio of the groove geometry and (ii) The contact angle of the underlying substrate material. For microfluidics, the most interesting shape regimes involve extended liquid filaments, which can grow and shrink in length while their cross section stays essentially constant. Thus, any method by which one can vary the contact angle can be used to switch the length of the filament, as is demonstrated in the context of electrowetting.  (+info)

Controlling nonspecific protein adsorption in a plug-based microfluidic system by controlling interfacial chemistry using fluorous-phase surfactants. (7/1368)

Control of surface chemistry and protein adsorption is important for using microfluidic devices for biochemical analysis and high-throughput screening assays. This paper describes the control of protein adsorption at the liquid-liquid interface in a plug-based microfluidic system. The microfluidic system uses multiphase flows of immiscible fluorous and aqueous fluids to form plugs, which are aqueous droplets that are completely surrounded by fluorocarbon oil and do not come into direct contact with the hydrophobic surface of the microchannel. Protein adsorption at the aqueous-fluorous interface was controlled by using surfactants that were soluble in fluorocarbon oil but insoluble in aqueous solutions. Three perfluorinated alkane surfactants capped with different functional groups were used: a carboxylic acid, an alcohol, and a triethylene glycol group that was synthesized from commercially available materials. Using complementary methods of analysis, adsorption was characterized for several proteins (bovine serum albumin (BSA) and fibrinogen), including enzymes (ribonuclease A (RNase A) and alkaline phosphatase). These complementary methods involved characterizing adsorption in microliter-sized droplets by drop tensiometry and in nanoliter plugs by fluorescence microscopy and kinetic measurements of enzyme catalysis. The oligoethylene glycol-capped surfactant prevented protein adsorption in all cases. Adsorption of proteins to the carboxylic acid-capped surfactant in nanoliter plugs could be described by using the Langmuir model and tensiometry results for microliter drops. The microfluidic system was fabricated using rapid prototyping in poly(dimethylsiloxane) (PDMS). Black PDMS microfluidic devices, fabricated by curing a suspension of charcoal in PDMS, were used to measure the changes in fluorescence intensity more sensitively. This system will be useful for microfluidic bioassays, enzymatic kinetics, and protein crystallization, because it does not require surface modification during fabrication to control surface chemistry and protein adsorption.  (+info)

High-throughput mouse genotyping using robotics automation. (8/1368)

The use of mouse models is rapidly expanding in biomedical research. This has dictated the need for the rapid genotyping of mutant mouse colonies for more efficient utilization of animal holding space. We have established a high-throughput protocol for mouse genotyping using two robotics workstations: a liquid-handling robot to assemble PCR and a microfluidics electrophoresis robot for PCR product analysis. This dual-robotics setup incurs lower start-up costs than a fully automated system while still minimizing human intervention. Essential to this automation scheme is the construction of a database containing customized scripts for programming the robotics workstations. Using these scripts and the robotics systems, multiple combinations of genotyping reactions can be assembled simultaneously, allowing even complex genotyping data to be generated rapidly with consistency and accuracy. A detailed protocol, database, scripts, and additional background information are available at  (+info)

Embodiments of the present invention provide improved microfluidic devices and related apparatus, systems, and methods. Methods are provided for reducing mixing times during use of microfluidic devices. Microfluidic devices and related methods of manufacturing are provided with increased manufacturing yield rates. Improved apparatus and related systems are provided for supplying controlled pressure to microfluidic devices. Methods and related microfluidic devices are provided for reducing dehydration of microfluidic devices during use. Microfluidic devices and related methods are provided with improved sample to reagent mixture ratio control. Microfluidic devices and systems are provided with improved resistance to compression fixture pressure induced failures. Methods and systems for conducting temperature controlled reactions using microfluidic devices are provided that reduce condensation levels within the microfluidic device. Methods and systems are provided for improved fluorescent imaging of
Microfluidic devices have a wide variety of biological applications. My Ph.D. dissertation focuses on three major projects. A) culturing a non-adherent immortal cell line within a microfluidic device under static and dynamic media flow conditions; B) designing and fabricating novel microfluidic devices for electrokinetic injecting analytes from a hydrodynamic fluid; and C) using this novel injection method to lyse single non-adherent cells by applying a high electric field across the cell at a microfluidic channel intersection. There are several potential advantages to the use of microfluidic devices for the analysis of single cells: First, cells can be handled with care and precision while being transported in the microfluidic channels. Second, cell culturing, handling, and analysis can be integrated together in a single, compact microfluidic device. Third, cell culturing and analysis in microfluidic devices uses only extremely small volumes of culturing media and analysis buffer. In this ...
TY - GEN. T1 - Microfluidic technology. T2 - new opportunities to develop physiologically relevant in vitro models integrated microfluidic platform for the in vitro pre-implantation culture of individual mammalian embryos and their in situ characterization. AU - le Gac, Severine PY - 2017/9/11. Y1 - 2017/9/11. U2 - 10.1109/ESSDERC.2017.8066641. DO - 10.1109/ESSDERC.2017.8066641. M3 - Conference contribution. SN - 978-1-5090-5979-9. SP - 260. EP - 263. BT - 47th European Solid-State Device Research Conference (ESSDERC 2017). PB - IEEE. CY - Piscataway, NJ. ER - ...
Droplet-based microfluidics has been used to facilitate high throughput analysis of individual prokaryote and mammalian cells. However, there is a scarcity of similar workflows applicable to rapid phenotyping of plant systems. We report on-chip encapsulation and analysis of protoplasts isolated from the emergent plant model Marchantia polymorpha at processing rates of ,100,000 protoplasts per hour. We use our microfluidic system to quantify the stochastic properties of a heat-inducible promoter across a population of transgenic protoplasts to demonstrate that it has the potential to assess gene expression activity in response to environmental conditions. We further demonstrate on-chip sorting of droplets containing YFP-expressing protoplasts from wild type cells using dielectrophoresis force. This work opens the door to droplet-based microfluidic analysis of plant cells for applications ranging from high-throughput characterisation of DNA parts to single-cell genomics ...
The use of microfluidic systems for screening of aptamers and their biomedical applications are reviewed in this paper. Aptamers with different nucleic acid sequences have been extensively studied and the results demonstrated a strong binding affinity to target molecules such that they can be used as promising candidate biomarkers for diagnosis and therapeutics. Recently, the aptamer screening protocol has been conducted with microfluidic-based devices. Furthermore, aptamer affinity screening by a microfluidic-based method has demonstrated remarkable advantages over competing traditional methods. In this paper, we first reviewed microfluidic systems which demonstrated efficient and rapid screening of a specific aptamer. Then, the clinical applications of screened aptamers, also performed by microfluidic systems, are further reviewed. These automated microfluidic systems can provide advantages over their conventional counterparts including more compactness, faster analysis, less sample/reagent
The market study on Global Microfluidic Devices Market 2017 Research Report studies current as well as future aspects of the Microfluidic Devices Market primarily based upon factors on which the companies compete in the market, key trends and segmentation analysis. This report covers each side of the worldwide market, ranging from the fundamental market info and advancing more to varied important criteria, based on that, the Microfluidic Devices market is segmented. Microfluidic Devices industry research report analyzes, tracks, and presents the global market size of the major players in every region around the world. Furthermore, the report provides data of the leading market players in the Microfluidic Devices market.. This report studies Microfluidic Devices in Global market, especially in North America, China, Europe, Southeast Asia, Japan and India, with production, revenue, consumption, import and export in these regions, from 2012 to 2017, and forecast to 2022.. Request for FREE Sample ...
misc{8894052, abstract = {Microfluidic applications nowadays have become of great interest due to their broad compatibility especially in biological applications, and one of them being droplet-based cell encapsulation. Cell encapsulation in droplets is carried out by discretising an aqueous phase (i.e. cell suspension) and including them into a continuous oil phase. This methodology is a potential gateway to high throughput droplet-based cell fusion (e.g. for the production of hybridomas). The challenge here is to achieve a high efficiency of correctly paired cells in a droplet to overcome the random fusion pairing during bulk cell fusion. As such, droplet microfluidics can be used to co-encapsulate a single cell A and a single cell B cells in one droplet or encapsulate cells separately and merge droplets with desired cell number and type subsequently with other droplet manipulations. In this study, separate encapsulation studies of human B lymphocytes and mouse embryonic stem cells were ...
Laboratory of microfluidic technologies for biomedicine: MicroRNA, miRNA, organ-on-chip, microfluidics, toxin, viscumin, ricin, laminin, placenta, intestine, gut, liver, brain, blood-brain barrier, cancer, tumor
Microfluidic devices are analogous to circuit boards, and they can be programmed to perform all kinds of laboratory tasks on a small scale. They have the potential to perform all kinds of medical tests involving body fluids in a short time and using very small samples.. While circuit boards pass electricity, which can be abstracted and quantified as bits, microfluidic devices tend to work with liquids that can mix with one another and contaminate each other. For microfluidic devices to approach the logic abilities of circuit boards, the fluids within have to somehow be perfectly separated from each other until the time that theyre expected to mix. Conventional microfluidic gates and valves arent adequate in this context, so researchers at Duke University have now developed a way to keep individual droplets from touching each other while moving them around using sound waves inside a microfluidic device.. Scaling up this approach could lead to programmable and rewritable microfluidics that can ...
A system and method for integrating microfluidic components in a microfluidic system enables the microfluidic system to perform a selected microfluidic function. A capping module includes a microfluidic element for performing a microfluidic function. The capping module is stacked on a microfluidic substrate having microfluidic plumbing to incorporate the microfluidic function into the system. The microfluidic element may comprise a matrix having an affinity for selected molecules in a sample. The matrix binds, reacts with and/or retains the selected molecules without affecting other molecules in the sample.
A system and method for integrating microfluidic components in a microfluidic system enables the microfluidic system to perform a selected microfluidic function. A capping module includes a microfluidic element for performing a microfluidic function. The capping module is stacked on a microfluidic substrate having microfluidic plumbing to incorporate the microfluidic function into the system. The microfluidic element may comprise a matrix having an affinity for selected molecules in a sample. The matrix binds, reacts with and/or retains the selected molecules without affecting other molecules in the sample.
Optical coherence tomography (OCT) angiography (OCTA) has been actively studied as a noninvasive imaging technology to generate retinal blood vessel network maps for the diagnoses of retinal diseases. Given that the uses of OCT and OCTA have increased in the field of ophthalmology, it is necessary to develop retinal phantoms for clinical OCT for product development, performance evaluation, calibration, certification, medical device licensing, and production processes. We developed a retinal layer-mimicking phantom with microfluidic channels based on microfluidic fabrication technology using polydimethylsiloxane (PDMS) and titanium dioxide (TiO2) powder. We implemented superficial and deep retinal vessels using microfluidic channels. In addition, multilayered thin films were synthesized with multiple spin-coating processes that comprised layers that corresponded to the retinal layers, including the ganglion cell layer (GCL), inner plexiform layer (IPL), and inner nuclear layer (INL). The phantom ...
Agave BioSystems proposes to develop and demonstrate an innovative Organic Thin Film Transistor (OTFT) to detect cell characteristics without the need for cell labeling for use in microfluidic flow cytometers. This sensor would be able to detect count all cells, whether the cells are fluorescently labeled or not. This device would easily be able to detect the presence of cells within the channel as well as give information about the cell size and even the DNA content or intactness of the cell. The fact that cells will not need to be fluorescently labeled for detection is one of the major advantages of this device, since it can be used to measure live, unperturbed cells. Microfabrication of the OTFTs and microfluidic components will allow development of inexpensive, self-contained, disposable, high-throughput devices for screening of combinatorial chemical, biochemical or biological libraries. Assisting in this project will be Prof. George Malliaras of the Department of Materials Science and ...
Microfluidic gradient generators are used to study the movement of living cells, lipid vesicles, and colloidal particles in response to spatial variations in their local chemical environment. Such gradient driven motions are often slow (less than 1 μm s−1) and therefore influenced or disrupted by fluid flows Lab on a Chip Emerging Investigators Lab on a Chip Recent HOT Articles
A variety of pulmonary diseases such as COPD, asthma, ARDS are profoundly associated with the surfactant dysfunction that leads to liquid plug formation across the airway lumen [19]. Several animal model studies have shown during such lung disorders severe tissue-level damage to the distal lung airways due to repeated closure and reopening process [4]. To mimic exactly the in vivo conditions, Huh et al. [20] developed a compartmentalized microfluidic airway models and demonstrated that the reopening of occluded microfluidic airway causes severe injury of pulmonary epithelial cells [20]. In the lung airways, rupturing of the liquid plugs leads to abnormal breath sounds known as crackles. To simulate this scenario, a three-dimensional (3D) microfluidic device was developed to detect acoustically the crackling sound and it was demonstrated that there is a higher risk of cell injury when liquid plugs become very thin. They demonstrated cellular level of lung injury under flow condition using this ...
We have developed a microfluidic flow cell where stepwise enzymatic digestion is performed on immobilized proteoliposomes and the resulting cleaved peptides are analyzed with liquid chromatography-tandem mass spectrometry (LC-MS/MS). The flow cell channels consist of two parallel gold surfaces mounted face to face with a thin spacer and feature an inlet and an outlet port. Proteoliposomes (50-150 nm in diameter) obtained from red blood cells (RBC), or Chinese hamster ovary (CHO) cells, were immobilized on the inside of the flow cell channel, thus forming a stationary phase of proteoliposomes. The rate of proteoliposome immobilization was determined using a quartz crystal microbalance with dissipation monitoring (QCM-D) which showed that 95% of the proteoliposomes bind within 5 min. The flow cell was found to bind a maximum of 1 μg proteoliposomes/cm2, and a minimum proteoliposome concentration required for saturation of the flow cell was determined to be 500 μg/mL. Atomic force microscopy (AFM)
The hanging drops are connected through 200μm-wide channels (Image: Chemistry World) Scientists in Switzerland have incorporated pulsing human heart tissue into a microfluidic device to make a miniscule model of a living system that could be used to test new drugs.. Microfluidic technology manipulates tiny volumes of liquid. One of its most exciting applications lies in building models of the human body - so-called body-on-a-chip or microphysiological systems. Many microphysiological systems already allow small human tissue samples, which approximate the behaviour of whole organs, to be tested under different conditions. Models of multi-organ systems are linked by slender liquid channels, and the flow of liquid and metabolites from one sample to the next can be controlled with pumps and valves. However, a huge challenge for the concept is that - after the attritive processes of extraction, culturing and insertion into a microfluidic environment - the samples often behave quite differently to ...
Developing blood-based tests is appealing for non-invasive disease diagnosis, especially when biopsy is difficult, costly, and sometimes not even an option. Tumor-derived exosomes have attracted increasing interest in non-invasive cancer diagnosis and monitoring of treatment response. However, the biology and clinical value of exosomes remains largely unknown due in part to current technical challenges in rapid isolation, molecular classification and comprehensive analysis of exosomes. Here we developed a new microfluidic approach to streamline and expedite the exosome analysis pipeline by integrating specific immunoisolation and targeted protein analysis of circulating exosomes. Compared to the conventional methods, our approach enables selective subpopulation isolation and quantitative detection of surface and intravesicular biomarkers directly from a minimally invasive amount of plasma samples (30 μL) within ~100 min with markedly improved detection sensitivity. Using this device, we ...
TY - JOUR. T1 - An integrated microfluidic device for monitoring changes in nitric oxide production in single T-lymphocyte (Jurkat) cells. AU - Metto, Eve C.. AU - Evans, Karsten. AU - Barney, Patrick. AU - Culbertson, Anne H.. AU - Gunasekara, Dulan B.. AU - Caruso, Giuseppe. AU - Hulvey, Matthew K.. AU - Fracassi Da Silva, Jose Alberto. AU - Lunte, Susan M.. AU - Culbertson, Christopher T.. PY - 2013/11/5. Y1 - 2013/11/5. N2 - A considerable amount of attention has been focused on the analysis of single cells in an effort to better understand cell heterogeneity in cancer and neurodegenerative diseases. Although microfluidic devices have several advantages for single cell analysis, few papers have actually demonstrated the ability of these devices to monitor chemical changes in perturbed biological systems. In this paper, a new microfluidic channel manifold is described that integrates cell transport, lysis, injection, electrophoretic separation, and fluorescence detection into a single device, ...
Microfluidic devices offer the chance to manipulate and analyze fluids including bioassays and chemical reactions. In this study, a method to develop a microfluidic analysis system is proposed for detection of nanotubes by a Raman acquisition setup. Microchannels where fabricated in sodalime glass substrate by MeV ion beam lithography or electron beam lithography and wet etching. Fusion bonding (550 °C) was used to seal the microchannels. As a result a prototype microfluidic device with 1.6 µm deep channel that exhibit efficient sealing and suitable channel geometry was obtained. The microfluidic device was tested in a Raman spectroscopy detection system and the collected spectra showed the presence of carbon nanotubes within the channel with clear RBM and G-band peaks. By this approach a practical and simple fabrication technique for microfluidic devices combined with Raman spectroscopy was done. This device can be enhanced to perform concentration maps within the channel and further research ...
Electrophoretic separation in nanofluidic channels exhibits significant differences with microfluidics. We discuss a theoretical / experimental collaboration investigating particle separation by electropohoresis in nanochannels. Recent experimental results in the laboratory of our collaborator Dr. Pennathur (UCSB, Dept. ME) indicate that increased fidelity can be achieved in separating particles by size and charge when using channels with cross sections of nanometer dimensions (100nm x 1000nm), as opposed to larger microfluidic channels. For short double-strands of DNA (10 - 100 base pairs) it is found that separation in microfluidic channels produces electropherograms with only one lumped peak. However, for nanofluidic channels several clearly distinct peaks are observed. Given the small dimensions of the nanofluidic channel, it is expected that new effects which were relatively weak in microfluidic channels play an important role. Identifying how these underlying mechanisms augment electrophoretic
Advances in cell biology, quantification, and identification procedures are essential to develop novel particle characterization tools on the diagnostics, biotechnology, pharmaceutical industry, and material science. Flow cytometry is a pivotal technology and meets the need for almost a century. Increase in todays demand for fast, precise, accurate, and low-cost point-of-care diagnostic tools and cell counting technologies necessitate further improvements for state-of-the-art flow cytometry platforms. These improvements are achievable using novel and precise particle focusing techniques, multiple detection methods, integrated fluidic, optical, and electronic units in the same workflow. Thanks to its indisputable advantages in such integrities, microfluidic flow cytometry platforms are attractive and promising tool for the future of next-generation flow cytometry technologies. In this thesis, we developed viscoelastic focusing technique compatible with optical, impedimetric, and imaging-based ...
A variable, closed-loop apparatus for regulating a microfluidic flow that employs a low-power deflection assembly, which is surface-mounted over a flexible membrane overlying a chamber integrated into a microfabricated platform. A flexible membrane, moveable between two positions, sealingly overlies the chamber. One of the positions of the membrane restricts the flow through the chamber to a greater degree than the other position. A deflection assembly disposed on the substrate over the membrane unidirectionally deflects the membrane, thereby regulating the flow through the chamber.
Culturing Pancreatic Islets in Microfluidic Flow Enhances Morphology of the Associated Endothelial Cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Nowadays, many researchers in the field of gene delivery are focused on develop methods to produce nanoparticles with physicochemical characteristics in reproducible, con..
Circular dichroism (CD) is the differential absorption of left- and right-handed circularly polarized light. It is a form of spectroscopy used to determine the optical isomerism and secondary structure of molecules, and to study a wide variety of chiral materials in solution, particularly biologically important molecules such as proteins, nucleic acids, carbohydrates, lipids and drugs. The benefit of carrying out such experiments using synchrotron radiation is that the light available is several orders of magnitude higher in intensity than that available using conventional CD instruments, thereby providing a much higher signal-to-noise ratio over a wide wavelength range (140-700 nm). This paper will detail the development of a technique for rapidly producing 3D printed microfluidic channels in transparent polymer flow cells that enables the rapid and low-cost evaluation and iteration of microfluidic channel geometries. Permitting the flow through novel microfluidic devices to be interrogated thoroughly
TY - JOUR. T1 - Detection of culture-negative sepsis in clinical blood samples using a microfluidic assay for combined CD64 and CD69 cell capture. AU - Zhou, Yun. AU - Zhang, Ye. AU - Johnson, Amanda. AU - Venable, Amanda. AU - Griswold, John. AU - Pappas, Dimitri. PY - 2019/7/25. Y1 - 2019/7/25. N2 - Sepsis is a life-threatening disease that affects millions of people every year. Rapid detection of sepsis assists clinicians to initiate timely antibiotic therapy and to reduce mortality. At the same time, accurate point-of-care detection is needed to reduce unnecessary use of antibiotics. One of the principal challenges in sepsis diagnosis is that many sepsis cases do not result in positive blood cultures. These so-called culture-negative cases present a significant health threat. In this work, we present a microfluidic cells separation system for the detection of sepsis in both culture-positive and culture-negative cases. Leukocytes were captured in several affinity separation zones of a ...
The main aim of this project was to develop novel concepts for miniaturization of bioanalytical techniques for investigating biomolecular interactions. We used optical tweezers to selectively address individual biological objects in microfluidic channels. A general introduction of applications of optical tweezers and microfluidics is given in chapter 1. Theoretical concepts related to optical trapping and microfluidics are reviewed in chapter 2, followed by a detailed description of the instrumentation in chapter 3. In chapter 4, ligand-receptor interactions are studied under physiological conditions: whole cells or native vesicles carrying in their membrane the protein of interest are immobilized first in the laser trap inside a microfluidic channel, then the reaction is initiated by changing the solution in the region around the trap. In chapter 5 and chapter 6 respectively, surface-modified polystyrene beads are used to study ligand-receptor interactions and DNA hybridization. The examples of ...
We provide custom prototyping of PDMS microfluidic devices and SU-8 master molds. This rapid prototyping method is ideal with its low-cost and quick turn-around time. Our experienced team will work with you to produce devices specifically tailored to your application. HJ Science & Technology, HJ Science, HJST microfluidic, automation, custom microfluidics, microfluidic manufacturing, microfluidic prototyping, contract manufacturing, microfluidic technology, microfluidic automation, valve-less fluidic switching, microfluidic devices, PDMS devices, SU-8 molds, PDMS, SU-8
The purpose of this thesis is to study the crystallization in a microfluidic device of an active pharmaceutical ingredient which is (2S)-2-[(4R)-2-oxo- 4-propylpyrrolidin-1-yl] butanamide, with product name Brivaracetam. This molecule is manufactured by the Belgian pharmaceutical industry UCB, and a better understanding of its crystallization in the microscopic scale, and especially its polymorphism, would lead to new possibilities in order to develop a future industrial continuous crystallizer based in the microfluidic technology. For this purpose, several experiments have been run, both in the macroscopic scale and using the microfluidics technology. The solubility curve for the system was determined, and also a cluster formation was analysed using volumes around 10ml of solution. Several crystallizations were done also with solutions of this volume to first understand the polymorphism that the solute presents. For the micro-scale crystallizations, the microfluidic device used was a system ...
TY - JOUR. T1 - Compartmentalized 3D Tissue Culture Arrays under Controlled Microfluidic Delivery. AU - Gümüscü, B.. AU - Albers, Hugo J.. AU - Van Den Berg, Albert. AU - Eijkel, Jan C.T.. AU - Van Der Meer, Andries D.. PY - 2017/12/1. Y1 - 2017/12/1. N2 - We demonstrate an in vitro microfluidic cell culture platform that consists of periodic 3D hydrogel compartments with controllable shapes. The microchip is composed of approximately 500 discontinuous collagen gel compartments locally patterned in between PDMS pillars, separated by microfluidic channels. The typical volume of each compartment is 7.5 nanoliters. The compartmentalized design of the microchip and continuous fluid delivery enable long-Term culturing of Caco-2 human intestine cells. We found that the cells started to spontaneously grow into 3D folds on day 3 of the culture. On day 8, Caco-2 cells were co-cultured for 36 hours under microfluidic perfusion with intestinal bacteria (E. coli) which did not overgrow in the system, and ...
TY - JOUR. T1 - DNA aptamer-based sandwich microfluidic assays for dual quantification and multi-glycan profiling of cancer biomarkers. AU - Jolly, Pawan. AU - Damborsky, P.. AU - Madaboosi, N.. AU - Soares, R.R.G.. AU - Chu, V.. AU - Conde, J.P.. AU - Katrlik, J.. AU - Estrela, Pedro. PY - 2016/5/16. Y1 - 2016/5/16. N2 - Two novel sandwich-based immunoassays for prostate cancer (PCa) diagnosis are reported, in which the primary antibody for capture is replaced by a DNA aptamer. The assays, which can be performed in parallel, were developed in a microfluidic device and tested for the detection of free Prostate Specific Antigen (fPSA). A secondary antibody (Aptamer-Antibody Assay) or a lectin (Aptamer-Lectin Assay) is used to quantify, by chemiluminescence, both the amount of fPSA and its glycosylation levels. The use of aptamers enables a more reliable, selective and controlled sensing of the analyte. The dual approach provides sensitive detection of fPSA along with selective fPSA ...
Accurate analysis at the single-cell level has become a highly attractive tool for investigating cellular content. An electroosmotic-driven microfluidic chip with arrays of 30-µm-diameter microwells was developed for single-cell electric lysis in the present study. The cellular occupancy in the microwells when the applied voltage was 5 V (82.4%) was slightly higher than that at an applied voltage of 10 V (81.8%). When the applied voltage was increased to 15 V, the cellular occupancy in the microwells dropped to 64.3%. More than 50% of the occupied microwells contain individual cells. The results of electric lysis experiments at the single-cell level indicate that the cells were gradually lysed as the DC voltage of 30 V was applied; the cell was fully lysed after 25 s. Single-cell electric lysis was demonstrated in the proposed microfluidic chip, which is suitable for high-throughput cell lysis.
Health, ...ANN ARBOR Mich. Cancer cells are on the move in the bloodstream in t...In a study of 51 patients researchers used a state-of-the art microfl...The findings published in Gastroenterology suggest that circu... While there is much work that still needs to be done there is great ...,Microfluidic,technology,reveals,potential,biomarker,for,early,pancreatic,cancer,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
A microsystem integrating electrochemical detection for the simultaneous detection of protein markers of breast cancer is reported. The microfluidic platform was realized by high precision milling of polycarbonate sheets and features two well distinguishable sections: a detection zone incorporating the elect
This paper reports on the electrochemical characterization and comparison of printable silver inks for the fabrication of planar electrodes to integrate in a microfluidic platform for in situ desalination of seawater prior to detection of nutrients in marine environment. Screen printing and inkjet printing were investigated to overcome limitations of more conventional deposition techniques. The screen printed ink DuPont 5064H was chosen for the fabrication of the desalination platform as it displayed the best properties in terms of oxidation ability (15.5 mC/mm(2)) and absence of sample contamination. The platform was tested at different oxidation conditions; the optimum potential for desalination was found to be +0.9 V. The device desalination performance was then evaluated through conductivity measurements of the treated sample and a proof-of-concept was achieved: for a potential of +0.9 V, conductivity (hence concentration) could be lowered from an initial value of 42.13 mS (0.6 M) to 20.36 ...
Video articles in JoVE about microfabrication include Microfabrication of Nanoporous Gold Patterns for Cell-material Interaction Studies, Microfabrication of Chip-sized Scaffolds for Three-dimensional Cell cultivation, Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening, Experimental Methods for Trapping Ions Using Microfabricated Surface Ion Traps, Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks, A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)-Cell Interaction and the Resultant Bioeffects at the Single-cell Level, Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles, Soft Lithographic Procedure for Producing Plastic Microfluidic Devices with View-ports Transparent to Visible and Infrared Light, A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological
Here a new approach to perform patch-clamp investigations under anoxic and normoxic conditions on nerve cells from Sprague Dawley rats is presented. A patch-clamp micropipette is integrated within a poly-methyl methacrylate (PMMA) based microchip giving optimal control over the oxygen content and the biochemical environment. Nerve cells were trapped by optical tweezers and steered towards the patch-clamp micropipette within the micro-channels. Several experiments were performed to show proof of principle. The oxygen content within the microfluidic chamber was measured to 0.5-1.5 %. The photo-induced effect of the optical tweezers on the nerve cells was investigated in an open Petri dish. The optical trapping did not influence measurements. The microfluidic system was further tested in patch-clamp experiments. This approach showed significant advantages regarding the tuning of the oxygen content and may be used in various electrophysiological investigations of single cells demanding optimal ...
Microfluidic devices can be used for many applications, including the formation of well-controlled emulsions. In this study, the capability to continuously create mono-disperse droplets in a microfluidic device is used to form calcium-alginate capsules through chemical crosslinking from aqueous droplets of calcium chloride and sodium alginate suspended in an oil solution. Calcium-alginate capsules have many potential uses, such as immunoisolation of cells, microencapsulation of active drug ingredients, and encapsulation of bitter agents in food or beverage products. Capsule formation is accomplished through fusion of a sodium alginate droplet and a calcium chloride droplet. The high surface tension between the droplet of calcium chloride and sodium alginate necessitates the use of the surfactant sodium dodecyl sulfate (SDS) and a device with a judiciously designed geometry. After creating the capsules, it is necessary to separate them out of the oil solution and into an aqueous solution. A ...
Microfluidic fluorescence assay devices show great promise as preclinical and clinical diagnostic instruments. Normally, fluorescence signals from microfluidic chips are quantified by analysis of images obtained with a commercial fluorescence microscope. This method is unnecessarily expensive, time consuming, and requires significant operator training, particularly when considering future clinical translation of the technology. In this work, we developed a dedicated low cost fluorescence microfluidic device reader (FMDR) to read sandwich immunofluorescence assay (sIFA) devices configured to detect vascular endothelial growth factor ligand concentrations in ocular fluid samples. Using a series of sIFA calibration standards and a limited set of human ocular fluid samples, we demonstrated that our FMDR reader has similar sensitivity and accuracy to a fluorescence microscope for this task, with significantly lower total cost and reduced reading time. We anticipate that the reader could be used with ...
In order to overcome these challenges, we aim to develop a novel breast cancer model that incorporates the relevant properties of the three-dimensional microenvironment. For this purpose, we use microfluidic technology, which enables us to manipulate and control fluids at the small scale. Cell encapsulation is used to first generate soft, cell-containing beads that mimic the basement membrane. These beads are then embedded in a more fibrous matrix that mimics the stromal ECM, completing the tissue model.
The ability to control the deposition and location of adherent and non-adherent cells within microfluidic devices is beneficial for the development of micro-scale bioanalytical tools and high-throughput screening systems. Here, we introduce a simple technique to fabricate poly(ethylene glycol) (PEG) microstructures within microfluidic channels that can be used to dock cells within pre-defined locations. Microstructures of various shapes were used to capture and shear-protect cells despite medium flow in the channel. Using this approach, PEG microwells were fabricated either with exposed or non-exposed substrates. Proteins and cells adhered within microwells with exposed substrates, while non-exposed substrates prevented protein and cell adhesion (although the cells were captured inside the features). Furthermore, immobilized cells remained viable and were stained for cell surface receptors by sequential flow of antibodies and secondary fluorescent probes. With its unique strengths in utility and ...
Microlytic was founded in 2006 with the intention to use microfluidic technology to help solve the major problems in structural biology. Microlytic sought to develop and produce microfluidic chips that offered a high probability of crystallizing target proteins with a set-up that is simple and easy to use.. Microlytic developed microfluidic chips to let users grow crystals large enough to be used immediately for X-ray diffraction, plus give users direct access to the crystals themselves. These goals were realized with the development of the Crystal Former - the first microfluidic platform to allow users to go from crystallization screen to structure using a single device.. The advantages of the Crystal Former are ...
Fabrication of conductive pathways, microcircuits and microstructures in microfluidic networks - Disclosed herein are a variety of microfluidic devices and solid, typically electrically conductive devices that can be formed using such devices as molds. In certain embodiments, the devices that are formed comprise conductive pathways formed by solidifying a liquid metal present in one or more microfluidic channels (such devices hereinafter referred to as microsolidic devices). In certain such devices, in which electrical connections can be formed and/or reformed between regions in a microfluidic structure; in some cases, the devices/circuits formed may be flexible and/or involve flexible electrical components. In certain embodiments, the solid metal wires/conductive pathways formed in microfluidic channel(s) may remain contained within the microfluidic structure. In certain such embodiments, the conductive pathways formed may be located in proximity to other microfluidic channel(s) of the ...
The invention provides a microfluidic device having a plurality of chambers each containing separately deposited reagents. The invention also provides an efficient PCR-based method for producing a linear expression template. The invention also provides methods for analyzing interactions between molecules, involving flow-deposition of expression templates on the substrate of chambers in a microfluidic device, and expressing proteins from the templates.
Droplet-based microfluidics manipulate discrete volumes of fluids in immiscible phases with low Reynolds number and laminar flow regimes. Interest in droplet-based microfluidics systems has been growing substantially in past decades. Microdroplets offer the feasibility of handling miniature volumes (μl to fl) of fluids conveniently, provide better mixing, encapsulation, sorting, sensing and are suitable for high throughput experiments. Two immiscible phases used for the droplet generation are referred to as the continuous phase (medium in which droplets are generated) and dispersed phase (the droplet phase). The size of the generated droplets is mainly controlled by the flow rates of the continuous phase and dispersed phase, interfacial tension between two phases and the geometry used for the droplet generation. Generally, three types of microfluidic geometries are utilized for the droplet generation: (i) T-Junction, (ii) Flow Focusing, and (iii) Co-Flowing. The benefits of microfluidics can be ...
Synthetic biology aims to engineer biological systems for desired behaviors. The construction of these systems can be complex, often requiring genetic reprogramming, extensive de novo DNA synthesis, and functional screening. Herein, we present a programmable, multipurpose microfluidic platform and associated software and apply the platform to major steps of the synthetic biology research cycle: design, construction, testing, and analysis. We show the platforms capabilities for multiple automated DNA assembly methods, including a new method for Isothermal Hierarchical DNA Construction, and for Escherichia coli and Saccharomyces cerevisiae transformation. The platform enables the automated control of cellular growth, gene expression induction, and proteogenic and metabolic output analysis. Taken together, we demonstrate the microfluidic platforms potential to provide end-to-end solutions for synthetic biology research, from design to functional analysis.
View more ,Bioaffinity mass spectrometry screening is a novel approach using non-denaturing electrospray ionization (ESI) mass spectrometry (MS) in identifying drug leads. This screening technique can detect and preserve noncovalent protein-active drug ligand complexes under different physiological conditions. Although there are many successful screening campaigns employing this technique, the big challenge of the screening is the reduction of sample volume needed. We demonstrate in this paper that analysis of samples can be performed using droplet-based microfluidics. Droplets of samples to be screened are formed and delivered directly into the electrospray emitter of a Fourier Transform mass spectrometer. The results show that a MS instrument with a conventional ESI source can clearly detect the samples and distinguish it with the separating oil phase. The proposed technique opens the possibility of bioaffinity mass spectrometry screening of small samples with a simple microfluidic device ...
Polymers have assumed the leading role as substrate materials for microfluidic devices in recent years. They offer a broad range of material parameters as well as material and surface chemical properties which enable microscopic design features that cannot be realised by any other class of materials. A similar range of fabrication technologies exist to generate microfluidic devices from these materials. This review will introduce the currently relevant microfabrication technologies such as replication methods like hot embossing, injection molding, microthermoforming and casting as well as photodefining methods like lithography and laser ablation for microfluidic systems and discuss academic and industrial considerations for their use. A section on back-end processing completes the overview. ...
Microfluidics based Lab-on-a-chip technology exhibits an unprecedented perspective in studying microbiology and biochemistry. With microfluidic technology, researchers can manipulate and probe individual cells, and can precisely control their microenvironments. Thus microfluidics enables quantitative measurements with high biological/chemical selectivity and sensitivity, as well as high temporal and spatial resolution. This IDI proposal envisages Western becoming a national leader in multidisciplinary research and education in bionanotechnology and related subjects. As a part of the Westerns overall efforts to accomplish this, the research team plans to apply microfluidic technology to answer essential microbiology questions.. The combination of microfluidics and microbiology also opens a brand new field for education, specifically, bionanotechnology. In the aspect of education, the research team aims:. ...
In recent years there has been an increasing interest in using lipid vesicles and related membrane structures as (i) artificial cells that mimic biological processes and (ii) bio-inspired micro-machines that serve functional purposes. To date, vesicles have largely been single-compartment structures with homogenous interiors, which has impeded the fulfilment of these goals. This thesis details the development of technologies to address this. We develop droplet-based methods to controllably generate multi-compartment vesicles (MCVs) for the first time. The potential of these novel structures as artificial cells capable of hosting a range of biological and bio-mimetic processes is explored. Most notably, we introduce spatial segregation of function, thus mimicking eukaryotic organelles, and incorporate an artificial enzymatic signalling cascade to transmit chemical signals between distinct vesicle regions. We also construct microfluidic devices to generate related structures known as multisomes. ...
Plasma is a host of various analytes such as proteins, metabolites, circulating nucleic acids (CNAs), pathogens. The key process of plasma extraction is to eliminate the contamination from blood cells. Conventional methods, such as centrifugation and membrane filtration, are generally lab-intensive, time consuming and even dangerous. In this study, we report an integrated microfluidic device that combines inertial microfluidics and membrane filter. The integrated microfluidic device was evaluated by the diluted (x1/10, x1/20) whole blood, and the quality of the extracted blood plasma was tested. It was found that quality of extracted blood plasma from integrated device was equivalent to that obtained by the centrifugation. This study demonstrates a significant progress towards the practical application of inertial microfluidics with membrane filter for high-throughput and high efficient blood plasma extraction ...
Identification of rare cells or molecules from a mixture population is important in biology such as identification of rare cancer cells or nucleic acid in early stage cancer diagnosis. Recent advances in droplet-based microfluidics and hydrogel barcoded microsphere to capture all the mRNA molecules in each cell in a single step enables scientists to identify cells based on their whole transcriptome information. However, due to the large number of sequencing reads required to cover the whole transcriptome, this limits the number of cells processed in one sequencing run. We address this problem by using a stepwise approach by first encapsulating single cell and lysis buffer together in a water-in-oil picoliter droplet, then amplifying only the target DNA/RNA molecule of interest in each droplet, pico-inject hydrogel barcoded microsphere into each droplet to tag the amplicons prior to next generation sequencing. We demonstrated the use of this technology by applying it to study how single tumor ...
This high-quality international symposium will bring together leading experts in all fields of droplet-based microfluidics from all around the world. We fully believe that all the presentations in this symposium will stimulate the exchange of ideas and experiences amongst all the participants. There will be four sequential sessions during the course of the two days with plenty of opportunities for discussion and interaction. The topics of the whole symposium include: ...
Crystallization from lipidic mesophase matrices is a promising route to diffraction-quality crystals and structures of membrane proteins. The microfluidic approach reported here eliminates two bottlenecks of the standard mesophase-based crystallization protocols: (i) manual preparation of viscous mesophases and (ii) manual harvesting of often small and fragile protein crystals. In the approach reported here, protein-loaded mesophases are formulated in an X-ray transparent microfluidic chip using only 60 nL of the protein solution per crystallization trial. The X-ray transparency of the chip enables diffraction data collection from multiple crystals residing in microfluidic wells, eliminating the normally required manual harvesting and mounting of individual crystals. In addition, we validated our approach by on-chip crystallization of photosynthetic reaction center, a membrane protein from Rhodobacter sphaeroides, followed by solving its structure to a resolution of 2.5 Å using X-ray ...
We review microfluidic platforms that enable the miniaturization, integration and automation of biochemical assays. Nowadays nearly an unmanageable variety of alternative approaches exists that can do this in principle. Here we focus on those kinds of platforms only that allow performance of a set o …
Electrophoresis.. 12.1 Introduction.. 12.2 Experimental Section.. 12.3 Results and Discussion.. 12.4 Applications.. 12.5 Conclusions.. 13 Chemical Separations in 3D Microfluidics.. 13.1 Introduction.. 13.2 Fabrication.. 13.3 Results and Discussion on 3D Valves.. 13.4 Microfluidic Three-Dimensional Separation Columns.. 13.5 Results on Liquid Chromatography.. 13.6 Conclusions.. 14 Enabling Fundamental Research in Proteomics.. 14.1 Introduction.. 14.2 Membrane Protein Extraction.. 14.3 Conclusion.. PART IV BIOMEDICAL APPLICATIONS OF MICROFLUIDICS.. 15 Microengineering Neural Development.. 15.1 Introduction.. 15.2 Microengineering Guidance of Axons to their Targets.. 15.3 Synaptogenesis on a Microfluidic Chip.. 15.4 Conclusions.. 16 Applications of Centrifugal Microfluidics in Biology.. 16.1 Introduction.. 16.2 Why Use Centrifugal Force for Fluid Manipulation?. 16.3 How Centrifugal Microfluidic Platforms Work.. 16.4 CD Applications.. 16.5 Conclusions.. 17 Microfluidic Techniques for Point-of-Care In ...
Impedimetric measurement methods are a novel approach to the characterization of fluid in biological applications. Lab on a chip (LOAC) technologies could be combined with impedimetrics to benefit these applications. LOAC devices are currently being developed to pursue the miniaturization of larger scale processes. Current research shows great flexibility in using LOAC devices to reproduce biological processes such as those used in medical diagnostic applications. With a smaller form factor, testing that generally requires off-site lab usage can be deployed at the point-of-care. LOAC devices also have the potential to lower operating costs by reducing reagent volumes, labor costs, and cycle times.. Digital microfluidic devices (DMF) are one promising LOAC platform. These devices manipulate discrete droplets of fluid using electric fields. As such, DMF devices can create, move, merge, and mix droplets while eliminating mechanical components like channels, pumps, and valves. Manipulation of ...
MICROFLUIDIC PLATFORMS FOR CELL CULTURE AND MICROENVIRONMENT CONTROL By Yandong Gao Dissertation Submitted to the Faculty of the Graduate School of Vanderbilt University in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY in Mechanical Engineering December, 2011 Nashville, Tennessee Approved: Associate Professor Deyu Li Assistant Professor Joh F. Edd Assistant Professor Haoxiang Luo Professor Taylor G. Wang Assistant Professor Donna J. Webb To my beloved parents
This thesis contributes to the development of Lab-on-a-Chip systems that enables reliable, rapid medical diagnostics at the point-of-care. These contributions are focused on microfluidic Lab-on-a-Chip systems for sepsis diagnosis, autonomous sample-to-answer tests, and dried blood spot sampling.. Sepsis is a serious condition with high mortality and high costs for society and healthcare. To facilitate rapid and effective antibiotic treatment, improved sepsis diagnostics is needed. Diagnosis of sepsis requires the processing of relatively large blood volumes, creating a need for novel and effective techniques for the handling of large volume flows and pressures on chip. Components, materials, and manufacturing methods for pneumatically driven Lab-on-a-Chip systems have therefore been developed in this thesis. Microvalves, an essential component in many Lab-on-a-Chip systems have been the focus on several of the advances: a novel elastomeric material (Rubbery Off-Stoichiometric-Thiol-Ene-Epoxy) ...
We have evaluated double-stranded DNA separations in microfluidic devices which were designed to couple a sample preconcentration step based on isotachophoresis (ITP) with a zone electrophoretic (ZE) separation step as a method to increase the concentration limit of detection in microfluidic devices. Developed at ACLARA BioSciences, these LabCard™ devices are plastic 32 channel chips, designed with a long sample injection channel segment to increase the sample loading. These chips were designed to allow stacking of the sample into a narrow band using discontinuous ITP buffers, and subsequent separation in the ZE mode in sieving polymer solutions. Compared to chip ZE, the sensitivity was increased by 40-fold and we showed baseline resolution of all fragments in the ΦX174/HaeIII DNA digest. The total analysis time was 3 min/sample, or less than 100 min per LabCard device. The resolution for multiplexed PCR samples was the same as obtained in chip ZE. The limit of detection was 9 fg/μL of DNA ...
Inertial microfluidics has been broadly investigated, resulting in the development of various applications, mainly for particle or cell separation. Lateral migrations of these particles within a microchannel strictly depend on the channel design and its cross-section. Nonetheless, the fabrication of these microchannels is a continuous challenging issue for the microfluidic community, where the most studied channel cross-sections are limited to only rectangular and more recently trapezoidal microchannels. As a result, a huge amount of potential remains intact for other geometries with cross-sections difficult to fabricate with standard microfabrication techniques. In this study, by leveraging on benefits of additive manufacturing, we have proposed a new method for the fabrication of inertial microfluidic devices. In our proposed workflow, parts are first printed via a high-resolution DLP/SLA 3D printer and then bonded to a transparent PMMA sheet using a double-coated pressure-sensitive adhesive tape.
This study aims to develop droplet-based microfluidics for massively parallel single-cell genomics, to elucidate intra-tissue genetic heterogeneity at the single-cell resolution. Most tumors display extensive intra-tumor heterogeneity, with various subpopulations of cells contains different mutations. For understanding these intra-tissue heterogeneities, ideal single-cell genomics methods should analyze tens of thousands of single cells in an efficient manner. In this study, I will develop droplet-based microfluidic techniques to produce the compartmentalized reaction environments for single-cell sequencing.. ...
Microfluidics is the science and technology that deals with the flow of liquids from microliters (mL) to picoliters (pL) inside a micrometer sized channels1. The ability of microfluidics to miniaturize and mimic various laboratory procedures with limited space, great efficiency and low sample volumes these systems find use in various applications involving continuous flow microfluidics, droplet-based microfluidics, DNA chips (microarrays), molecular biology, etc.. A microfluidic chip is a set of microchannels molded into materials such as glass, silicon or polymers like polydimethyl siloxane (PDMS) 1.. Microfluidic platforms allow physicians to analyze a single drop of blood or urine sample for various markers of diseases without the need of sending comparatively large volumes of these samples to external laboratories for analysis2.. To determine a disease state, microfluidic sensors require specific disease-detecting biomolecules to be inserted into the platform of the system. These ...
Microfluidic mixing plays a key role in various fields, including biomedicine and chemical engineering. To date, although various approaches for imaging microfluidic mixing have been proposed, they provide only quantitative imaging capability and require exogenous labeling agents. Quantitative phase imaging techniques, however, circumvent these problems and offer label-free quantitative information about concentration maps of microfluidic mixing. We present the quantitative phase imaging of microfluidic mixing in various types of polydimethylsiloxane microfluidic channels with different geometries; the feasibility of the present method was validated by comparing it with the results obtained by theoretical calculation based on Fick's law. (C) 2017 Optical Society of ...
Adam R. Abate and David A. Weitz Syringe-vacuum microfluidics: A portable technique to create monodisperse emulsions Entry by [[Fei Pu]], AP 225, Fall 2012 Keywords: [[microfluidics]], [[emulsions]], [[monodisperse]] ==Summary== Monodisperse drop formation is the central operation in droplet-based microfluidics but can be quite challenging due to the need for precise, steady pumping of reagents; forming monodisperse drops with controlled properties is thus a stringent demonstration of the effectiveness of a control system.A simple method for creating monodisperse emulsions with microfluidic devices is presented. Unlike conventional approaches that require bulky pumps, control computers,and expertise with device physics to operate devices, this method requires only the microfluidic device and a hand-operated syringe. The fluids needed for the emulsion are loaded into the device inlets, while the syringe is used to create a vacuum at the device outlet; this sucks the fluids through the ...
Co-advisor: Brad Berron, Chemical Engineering. All living systems have a need to transport critical nutrients throughout their structures. This need is a critical challenge in the next generation of medical devices which use live cells to perform basic functions. It is also one of the primary challenges in engineering thick three-dimensional tissues. In these systems, the flow of nutrients needs to be uniform throughout the material at the micron-scale. In vivo, this is accomplished by an integrated circulatory system, but the detailed multi-scale geometry involved is particularly difficult to recreate ex vivo. In this project, we seek to use lithography-based microfabrication to generate 3D cell/hydrogel structures with embedded microfluidic channels.. Objective: To develop hydrogel-based microfluidic devices that mimic in vivo blood flow. Major project outcomes:. ...
In vitro compartmentalization (IVC) of reactions in bulk water-in-oil emulsions has been identified as a promising method for massively parallel processing (Griffiths & Tawfik 2006). In principle, such an approach allows access to the huge combinatorial parameter spaces required for screening, selecting and developing both natural and artificial biological and macromolecular systems by directed evolution (Kelly et al. 2007). For example, aqueous solutions containing a gene library could be emulsified with a homogenizer in an oil-surfactant mixture within a matter of minutes to produce a water-in-oil emulsion containing in excess of 1010 droplets per millilitre. Such a combinatorial approach would greatly benefit synthetic biology, providing a powerful paradigm in the characterization of biological systems. Each droplet would constitute an independent experiment where the inputs of the synthetic biology system would be stimulated in a specific way. A large collection of droplets could ...
TY - JOUR. T1 - All-in-one microfluidic assembly of insulin-loaded pH-responsive nano-in-microparticles for oral insulin delivery. AU - Costa, Clarinda. AU - Liu, Zehua. AU - Martins, João P.. AU - Correia, Alexandra. AU - Figueiredo, Patrícia. AU - Rahikkala, Antti. AU - Li, Wei. AU - Seitsonen, Jani. AU - Ruokolainen, Janne. AU - Hirvonen, Sami Pekka. AU - Aguiar-Ricardo, Ana. AU - Corvo, M. Luísa. AU - Santos, Hélder A.. N1 - UIDB/50006/2020 PD/BD/142880/2018 UID/DTP/04138/2020 PY - 2020/6/21. Y1 - 2020/6/21. N2 - Here, a continuous two-step glass-capillary microfluidic technique to produce a multistage oral delivery system is reported. Insulin is successfully encapsulated into liposomes, which are coated with chitosan to improve their mucoadhesion. The encapsulation in an enteric polymer offers protection from the harsh gastric conditions. Insulin permeability is enhanced across an intestinal monolayer.. AB - Here, a continuous two-step glass-capillary microfluidic technique to produce a ...
Bacterial chemotaxis, a remarkable behavioral trait which allows bacteria to sense and respond to chemical gradients in the environment, has implications in a broad range of fields including but not limited to disease pathogenesis, in-situ bioremediation and marine biogeochemistry. And therefore, studying bacterial chemotaxis is of significant importance to scientists and engineers alike. Microfluidics has revolutionized the way we study the motile behavior of cells by enabling observations at high spatial and temporal resolution in carefully controlled microenvironments. This thesis aims to explore the potential of microfluidic technology in studying bacterial behavior by investigating different aspects of bacterial chemotaxis on a microfluidic platform. We quantified population-scale transport parameters of bacteria using videomicroscopy and cell tracking in controlled chemoattractant gradients. Previously, transport parameters have been derived theoretically from single-cell swimming behavior ...
TY - JOUR. T1 - PDMS microchannel surface modification with teflon for algal lipid research. AU - Park, Jae Woo. AU - Na, Sangcheol. AU - Kang, Myeongwoo. AU - Sim, Sang Jun. AU - Jeon, Noo Li. PY - 2017/9/1. Y1 - 2017/9/1. N2 - This paper presents a simple method for modifying the polydimethylsiloxane (PDMS) microfluidic channels with Teflon for algal lipid research. When culturing and staining algae inside microfluidic devices, the small molecule dyes absorbed by the microchannel surface render it difficult for imaging and quantification. PDMS surface coated with Teflon-AF resists the absorption of hydrophobic dye molecules (i.e., BODIPY and Nile red) as confirmed using fluorescence microscopy. Here, we introduce a surface modification of PDMS microchannel using Teflon-AF using a procedure of filling and drying to directly treat the PDMS surface with perfluorinated materials. This method can be used to prevent the absorption of fluorescent probe and obtain clear fluorescence micrographs ...
TY - JOUR. T1 - Microfluidic-based multiplex immunoassay system integrated with an array of QD-encoded microbeads. AU - Han, Sang Won. AU - Jang, Eunji. AU - Koh, Won Gun. PY - 2015/3/31. Y1 - 2015/3/31. N2 - Here, we developed a multiplex immunoassay platform within microfluidic devices that combines suspension and the planar microarray format. For the suspension microarray format, QD-embedded polymeric microbeads with an average diameter of 24 μm were prepared using the Shirasu Porous Glass (SPG) membrane emulsification technique. To furnish the microbeads with resolvable spectral codes, QDs with two different colors (450 nm for blue and 520 nm for green) were used and different spectral codes were obtained by changing the ratio of emission intensity of the two different QDs within the microbeads. The surfaces of the QD-encoded microbeads were then functionalized with probe antibodies for immunoassays. The planar microarray format was achieved by an array of microholes fabricated in PDMS. ...
15th Internat.Conf.on Solid-State Sensors, Actuators and Microsystems (Transducers 2009), Denver, Colo., June 21-25, 2009 Technical Digest Piscataway, N.J. : IEEE, ...
This paper presents a new hermetic encapsulation method for negative-pressure-driven polydimethylsiloxane (PDMS) microfluidic devices. The hermetic materials used in this encapsulation are mainly para
Nanofluidic devices are structures having at least one dimension in the submicron range, which is of the same order of magnitude as the sizes of biomolecules and bioparticles such as proteins and viruses. As a result, size-selective separations are important applications for nanofluidics. Well-defined micro or nano device structures fabricated via micromachining have greatly reduced sample consumption and enabled separations in a parallel fashion, promising significant speed and resolution advantages over conventional size separation techniques, such as gel electrophoresis and size exclusion chromatography. In collaboration with others, I have developed a size separation method using nanofluidic devices consisting of an array of parallel planar nanochannels with varying heights. Separation of nanoparticles is accomplished by simply flowing a liquid suspension of the particles through the nanochannels via capillary action. When a mixture of particles arrives at an interface, where the channel steps from
This paper reports a novel method for the statistical analysis of quantum dot (QD) cytotoxicity and cellular uptake based on single cell cycles, which is part of a series of works on the study of QD cytotoxicity using a microfluidic system (Lab Chip, 2012, 12, 34743480; 2013, 13, 19481954). The specially designed microfluidic system consisted of a polydimethylsiloxane (PDMS) microwell array for single-cell arrangement and microchannels for QD solution diffusion, enabling effective control of stable cell density and the interdistance between them, as well as maintaining a constant QD concentration with no disturbance of the fluids which can affect cellular uptake. We showed that the treatment of QDs had no influence on cell cycles. However, the QD cytotoxicity was found to be dependent on cellular uptake in various cell cycle phases, because the accumulation and dilution of QDs happened in single cell cycles. The rank of QD cytotoxicity was G2/M > S > G0/G1. Thus, this technology could serve as a ...
The present invention relates to systems and methods for minimizing or eliminating diffusion effects. Diffused regions of a segmented flow of multiple, miscible fluid species may be vented off to a waste channel, and non-diffused regions of fluid may be preferentially pulled off the channel that contains the segmented flow. Multiple fluid samples that are not contaminated via diffusion may be collected for analysis and measurement in a single channel. The systems and methods for minimizing or eliminating diffusion effects may be used to minimize or eliminate diffusion effects in a microfluidic system for monitoring the amplification of DNA molecules and the dissociation behavior of the DNA molecules.
MicroFluidic Systems Has Received Over $45 Million in Government Contracts in the Last Ten YearsMicroFluidic Systems Biological Detection and Sample Prep Technologies Will Complement PositiveIDs Virus Detection and Diabetes Management Focus, While Providing Expanded Capabilities for Homeland Security Applications
Developing Microfluidic Systems for Proteome Analysis,” K. Jo, B.R. Reschke, X. Mao, R. L. Carroll, B. Edwards, and A. Timperman,Midwestern Universities Analytical Chemistry Conference, EastLansing, Michigan, December 4, 2009.. ...
TY - JOUR. T1 - Neurotrophin-mediated dendrite-to-nucleus signaling revealed by microfluidic compartmentalization of dendrites. AU - Cohen, Michael S.. AU - Orth, Carlos Bas. AU - Kim, Hyung Joon. AU - Jeon, Noo Li. AU - Jaffrey, Samie R.. PY - 2011/7/5. Y1 - 2011/7/5. N2 - Signaling from dendritic synapses to the nucleus regulates important aspects of neuronal function, including synaptic plasticity. The neurotrophin brain-derived neurotrophic factor (BDNF) can induce long-lasting strengthening of synapses in vivo and this effect is dependent on transcription. However, the mechanism of signaling to the nucleus is not well understood. Here we describe a microfluidic culture device to investigate dendrite-to-nucleus signaling. Using these microfluidic devices, we demonstrate that BDNF can act directly on dendrites to elicit an anterograde signal that induces transcription of the immediate early genes, Arc and c-Fos. Induction of Arc is dependent on dendrite- and cell body-derived calcium, whereas ...
0008] Another portable blood component analysis device has a microfluidic unit with a sample chamber; the sample chamber having a surface augmentation features with biospecific surface configured to capture particles from blood. an analyzer component having a signal detector and a receiving slot configured to receive the microfluidic unit and align the sample chamber with the signal detector; Preferably, the surface augmentation features include a packed bead bed with glass beads. The device claim 13, wherein the analyzer contains a pump configured to generate a vacuum of at least 10 kPa; Preferably, the surface augmentation features include a packed bead bed with glass beads with a diameter between 50 and 100 μm and the first longitudinal channel contains a narrow portion with a minimum dimension of less than the diameter of the glass beads. Preferably, the device includes a controller and a solar power source connected to power the pump and controller. Preferably, the microfluidic unit ...
Louis Jun Ye Ong, Lor Huai Chong, Lin Jin, Pawan Kumar Singh, Poh Seng Lee, Hanry Yu, Abhishek Ananthanarayanan, Hwa Liang Leo, Yi-Chin Toh. The practical application of microfluidic liver models for in vitro drug testing is partly hampered by their reliance on human primary hepatocytes, which are limited in number and have batch-to-batch variation. Human stem cell-derived hepatocytes offer an attractive alternative cell source, although their 3D differentiation and maturation in a microfluidic platform have not yet been demonstrated. We develop a pump-free microfluidic 3D perfusion platform to achieve long-term and efficient differentiation of human liver progenitor cells into hepatocyte-like cells (HLCs). The device contains a micropillar array to immobilize cells three-dimensionally in a central cell culture compartment flanked by two side perfusion channels. Constant pump-free medium perfusion is accomplished by controlling the differential heights of horizontally orientated inlet and outlet ...
To demonstrate the power of multilayer soft lithography, we fabricated active valves and pumps. Monolithic elastomeric valves and pumps, like other mechanical microfluidic devices, avoid several practical problems affecting flow systems based on electroosmotic flow (8,9, 20, 27-29) or dielectrophoresis (30, 31), such as electrolytic bubble formation around the electrodes and a strong dependence of flow on the composition of the flow medium (32-34). Electrolytic bubble formation, although not a problem for laboratory devices, seriously restricts the use of electroosmotic flow in integrated microfluidic devices. Also, neither electroosmotic nor dielectrophoretic flow can easily be used to stop flow or balance pressure differences.. We fabricated our valves using a crossed-channel architecture (Fig. 1A). Typical channels are 100 μm wide and 10 μm high, making the active area of the valve 100 μm by 100 μm. The membrane of polymer between the channels is engineered to be relatively thin ...
Dive into the research topics of A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microfluidic pores. Together they form a unique fingerprint. ...
We detail a method to fabricate three-dimensional paper-based microfluidic devices for use in the development of immunoassays. Our...
The present invention relates to methods and systems for delivering microwave radiation, e.g., for heating, to a microfluidic device. The microfluidic device of the present invention contains a microwave integrated circuit (MMIC) for applying microwave radiation to specific areas within the microfluidic device. The circuit preferably includes a transmission line on one surface of the microfluidic device and a ground plane on the opposing surface.
... a microfluidic platform for university-level analytical chemistry laboratories". Lab Chip. 12: 696-701. doi:10.1039/C2LC20951A. ... "Suspension flow in microfluidic devices--a review of experimental techniques focussing on concentration and velocity gradients ... "Development and applications of a microfluidic reactor with multiple analytical probes". Analyst. 137: 444-450. Bibcode:2012Ana ... Microfluidic cell culture. References[edit]. *^ S.C.Terry, J.H.Jerman and J.B.Angell:A Gas Chromatographic Air Analyzer ...
Microfluidic Paper-based Analytical Devices". Analytical Chemistry. 82 (1): 3-10. doi:10.1021/ac9013989. PMID 20000334. Osborn ... Therefore, microfluidic devices require alternative flow control techniques, a number of which are currently popular: One ... Foudeh, Amir M.; Didar, Fohid Fatanat; Veres, Teodor; Tabrizian, Maryam (2012). "Microfluidic designs and techniques using lab- ... Optical detection includes fluorescence-based techniques, chemiluminescence-based techniques, and surface plasmon resonance ( ...
... microfluidic analytical techniques MeSH E05.196.630.465.340 - electrophoresis, microchip MeSH E05.196.630.570 - microarray ... embryo culture techniques MeSH E05.200.249.484 - organ culture techniques MeSH E05.200.249.617 - tissue culture techniques MeSH ... cell culture techniques MeSH E05.200.249.374 - coculture techniques MeSH E05.200.249.437 - diffusion chambers, culture MeSH ... fluorescent antibody technique MeSH E05.200.750.551.512.240.300 - fluorescent antibody technique, direct MeSH E05.200.750.551. ...
Ng AH, Uddayasankar U, Wheeler AR (June 2010). "Immunoassays in microfluidic systems. Analytical and bioanalytical chemistry". ... "On-chip drop-to-drop liquid microextraction coupled with real-time concentration monitoring technique". Analytical Chemistry. ... "A fluorogenic heterogeneous immunoassay for cardiac muscle troponin cTnI on a digital microfluidic device". Analytical and ... "A highly efficient bead extraction technique with low bead number for digital microfluidic immunoassay". Biomicrofluidics. 10 ( ...
Kung, Chia-Te; Hou, Chih-Yao; Wang, Yao-Nan; Fu, Lung-Ming (2019-12-12). "Microfluidic paper-based analytical devices for ... and has since been used for techniques such as paper chromatography and lateral flow assays. However, it was only identified as ... Akyazi, Tugce; Basabe-Desmonts, Lourdes; Benito-Lopez, Fernando (2018-02-25). "Review on microfluidic paper-based analytical ... "Advances in Microfluidic Paper-Based Analytical Devices for Food and Water Analysis". Micromachines. 7 (5): 86. doi:10.3390/ ...
There is no common analytical approach for quantitation due to the constraints of traditional techniques (e.g. the limited ... Microfluidic modulation spectroscopy is an automated technique that overcomes these challenges of both FTIR and CD for use in ... Microfluidic modulation spectroscopy (MMS) is an infrared spectroscopy technique that is used to characterize the secondary ... Formulation scientists use a core set of analytical techniques to quantify the colloidal, chemical and conformational stability ...
Functionalization of porous surfaces have seen great success with high temperature photografting techniques. In microfluidic ... In industrial corona and plasma processes, cost-efficient and rapid analytical methods are required for confirming adequate ... This grafting technique allows for excellent control over the peptide composition as the bonded chain can be washed without ... These techniques provide characterization at surface depths of 1-10 nanometers, approximately the range of oxidation in plasma ...
Due to this polymerization technique, the paper microfluidic device could be folded using origami, allowing for both horizontal ... Liu, Shuopeng; Su, Wenqiong; Ding, Xianting (2016-12-08). "A Review on Microfluidic Paper-Based Analytical Devices for Glucose ... This technique has high resolution and is quick, but has high equipment and material costs. This technique utilizes a DLP ... Asano, Hitoshi; Shiraishi, Yukihide (2015-07-09). "Development of paper-based microfluidic analytical device for iron assay ...
Microfluidic devices can combine several analytical steps into one device. This technology has been coined by some as the "lab ... Microfluidic whole genome haplotyping is a technique for the physical separation of individual chromosomes from a metaphase ... Like with the microfluidic technique, specialized amplification platforms are necessary to address the problem of a small ... Most molecular biology techniques for haplotyping can accurately determine haplotypes of only a limited region of the genome. ...
Analytical Electrochemistry (3rd Ed, 2006) Biosensors and Chemical Sensors Biosensors for Direct Monitoring of Environmental ... His contributions in these directions have been of major impact in the development of electrochemical sensing techniques and ... microfluidic ("Lab-on-a-Chip") devices, and remote sensors for environmental and security monitoring. ... 1] DAC Award in Chemical Instrumentation, ACS Division of Analytical Chemistry, American Chemical Society. Accessed July 15, ...
2006). Microfluidic Techniques: Reviews and Protocols. Methods in Molecular Biology. Humana Press. ISBN 9781588295170. " ... 2018 American Chemical Society Division of Analytical Chemistry Award in Electrochemistry 2018 American Association for the ... Young Investigator Award 2006 United States Department of Defense Okaloosa Award 2006 Springer Nature Microfluidic Techniques ... "2018 Division Award Winners - ACS Division of Analytical Chemistry". Retrieved 2019-04-13. Science, American ...
GC-MS is a technique utilized in many analytical laboratories and is a very effective and adaptable analytical tool. Liquid ... Sharif KM, Chin ST, Kulsing C, Marriott PJ (September 2016). "The microfluidic Deans switch: 50 years of progress, innovation ... Different combinations of one dimensional GC and LC produced the analytical chromatographic technique that is known as two- ... Comprehensive two-dimensional gas chromatography is an analytical technique that separates and analyzes complex mixtures. It ...
OI Analytical, in its gas diffusion amperometric total cyanide method, uses a segmented flow injection analysis technique that ... Microminiaturized chromatography is carried out on microcolumns that are automatically renewed by microfluidic manipulations. ... Alpkem was purchased by Perstorp Group, and then later by OI Analytical in College Station Texas. OI Analytical manufactures ... The Bran+Luebbe CFA business was bought by SEAL Analytical in 2006 and they continue to manufacture, sell and support the ...
... micrototal analytical systems or lab-on-a-chip structures. For instance, NCAMs, when incorporated into microfluidic devices, ... Standard photolithography, bulk or surface micromachining, replication techniques (embossing, printing, casting and injection ... One of the more promising areas of nanofluidics is its potential for integration into microfluidic systems, i.e. ... Analytical Chemistry. 71 (21): 4913-4918. doi:10.1021/ac990615i. PMID 21662836. Kuo, T. C.; Sloan, L. A.; Sweedler, J. V.; Bohn ...
This technique can be used as an alternative to microchannels and microvalves for manipulation of substrates, allowing for an ... In recent years, attention has been drawn to using SAWs to drive microfluidic actuation and a variety of other processes. Owing ... TrAC Trends in Analytical Chemistry. 29 (2): 141-157. doi:10.1016/j.trac.2009.11.002. Sesen, Muhsincan; Alan, Tuncay; Neild, ... PDMS (polydimethylsiloxane) is a material that can be used to create microchannels and microfluidic chips. It has many uses, ...
Mass spectrometry techniques have become important analytical tools for proteomic and metabolomic analysis of single cells. ... The development of hydrodynamic-based microfluidic biochips has been increasing over the years. In this technique, the cells or ... The development of hydrodynamic-based microfluidic biochips has been increasing over the years. In this technique, the cells ... Researchers are trying to develop a technique that can fulfil what current techniques are lacking: high throughput, higher ...
February 2007). "Gravity-driven microfluidic particle sorting device with hydrodynamic separation amplification". Analytical ... simulated moving bed technique was proposed. In the simulated moving bed technique instead of moving the bed, the sample inlet ... He developed the technique, he coined chromatography, in the first decade of the 20th century, primarily for the separation of ... Analytical chromatography is done normally with smaller amounts of material and is for establishing the presence or measuring ...
Microfluidic pipette[edit]. A Microfluidic pipette, housed in a manifold holder. The colored solutions highlight the solutions ... Technique: Over-rotated forearm and wrist. Rotation of the forearm in a supinated position (palm up) and/or wrist flexion ... Analytical Chemistry. 82 (11): 4529-4536. doi:10.1021/ac100480f. PMID 20443547.. ... Technique: Elbow flexion or abduction. Arm strength diminishes as elbow posture is deviated from a 90° position.. Corrective ...
A key theme of deMello's research has been the development of ultra-high sensitivity detection methods for use in microfluidic ... More recently, his group have introduced the technique of stroboscopic imaging flow cytometry, which allows for high resolution ... and in 1997 moved back to his alma mater to take up the AstraZeneca Lectureship in Analytical Sciences at Imperial College ... His group has pioneered the use of microfluidic systems for small molecule chemistry and nanomaterial synthesis, and in recent ...
ISBN 1-57444-572-3. Minteer, Shelley D. (2006). Microfluidic Techniques: Reviews and Protocols. Humana Press. ISBN 1-59259-997- ... This device represents a key technology to fields such as chemical industry, pharmaceutical industry, analytical chemistry, ... ISBN 978-0-387-28597-9. Li, Paul C. H. (2005). Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery. ... ISBN 978-1-58053-972-2. Hardt, Steffen & Schönfeld, Friedhelm (2007). Microfluidic Technologies for Miniaturized Analysis ...
Research on microfluidic found its advantages in DNA analysis, lab-on-a-chip, and micro-TAS. Devices in a microfluidic system ... Advances in nanofabrication techniques and concerns about energy shortage make people interested in this idea. The main ... The future of nanofluidic systems will be focused on several areas such as analytical chemistry and biochemistry, liquid ... Integration of these microfluidic devices enables sorting, transporting, and mixing of substances within fluids. However, the ...
... is a matrix-free electrophoretic separation technique. FFE is an analogous technique to capillary electrophoresis, with a ... Preparative as well as analytical operation modes Supports all electrophoretic separation modes (IEF, IZE, ZE, ITP) Can be run ... Multistep liquid-phase lithography for fast prototyping of microfluidic free-flow-electrophoresis chips, Anal. Bioanal. Chem., ... Because of the versatility of the technique, a wide range of protocols for the separation of samples like rare metal ions, ...
Common techniques include: Fluorescence activated cell sorting (FACS) Microfluidic devices To measure the level of expression ... Stegle, Oliver; Teichmann, Sarah A.; Marioni, John C. (1 March 2015). "Computational and analytical challenges in single-cell ... Techniques used for analysing RNA-seq data from bulk cell populations can be used for single-cell data but many new ... Common techniques for measuring expression are quantitative PCR or RNA-seq. There are several methods available to isolate and ...
... analytical techniques], but to me, they're tools. They're not ends in themselves... With new tools and measurement techniques, ... Wu, H.; Wheeler, A.; Zare, R. N. (24 August 2004). "Chemical cytometry on a picoliter-scale integrated microfluidic chip" (PDF ... LIF is an extremely sensitive technique with applications ranging from analytical chemistry and molecular biology to ... Zare earned his B.A. in chemistry and physics in 1961 and his Ph.D. in 1964 in physical and analytical chemistry at Harvard ...
The centrifugal micro-fluidic biochip or centrifugal micro-fluidic biodisk is a type of lab-on-a-chip technology, also known as ... The micromachining techniques, including patterning, photolithography, and etching should all be used as long as the design is ... Morais, Sergi (2008). "Analytical prospect of compact disk technology in immunosensing". Anal Bioanal Chem. 391 (8): 2837-2844 ... Once the centrifugal micro-fluidic biochip is developed well enough to be manufactured on a large scale, it will cause a wide ...
Some new instrumentations techniques exist that allow zeta potential to be measured. The Zeta Potential Analyzer can measure ... There are several analytical theories that incorporate surface conductivity and eliminate the restriction of the small Dukhin ... Transport in Microfluidic Devices. Cambridge University Press. ISBN 978-0-521-11903-0.[page needed] Greenwood, R.; Kendall, K ... All these measuring techniques may require dilution of the sample. Sometimes this dilution might affect properties of the ...
Meyvantsson I, Beebe DJ (2008-06-13). "Cell culture models in microfluidic systems". Annual Review of Analytical Chemistry. 1 ( ... This technique has demonstrated a stark difference in the sensitivity of the peripheral terminals compared to the neuronal cell ... Since the advent of poly(dimethylsiloxane) (PDMS) microfluidic device fabrication through soft lithography microfluidic devices ... and direct output to analytical instruments. Additionally, open microfluidic cell cultures such as "microcanals" allow for ...
Yu Z, Lu S, Huang Y (October 2014). "Microfluidic whole genome amplification device for single cell sequencing". Analytical ... Multiple displacement amplification (MDA) is a widely used technique, enabling amplifying femtograms of DNA from bacterium to ... MALBAC has also been implemented in a microfluidic device, but the amplification performance was not significantly improved by ... While performing MDA with a microfluidic device markedly reduces bias and contamination, the chemistry involved in MALBAC does ...
7.4 Microfluidic Sanger sequencing. *7.5 Microscopy-based techniques. *7.6 RNAP sequencing. *7.7 In vitro virus high-throughput ... Analytical Biochemistry. 344 (1): 53-69. doi:10.1016/j.ab.2005.05.028. PMID 16054106.. ... Microfluidic Sanger sequencing[edit]. Main article: Sanger sequencing. In microfluidic Sanger sequencing the entire ... Microscopy-based techniques[edit]. Main article: Transmission electron microscopy DNA sequencing. This approach directly ...
The most used techniques are collection rate measurements: this is the simplest and most used technique - electrodes are ... Kirby, B. J. (2010). Micro- and Nanoscale Fluid Mechanics: Transport in Microfluidic Devices. Cambridge University Press. ISBN ... Analytical Chemistry. 80 (6): 2063-8. doi:10.1021/ac702083g. PMID 18278948.. ... this technique is used for smaller particles (e.g. viruses), that are difficult to count with the previous technique;[30] ...
"Microfluidic impedance-based flow cytometry". Cytometry Part A. 77A (7): 648-666. doi:10.1002/cyto.a.20910.. ... The technique was expanded by Len Herzenberg, who was responsible for coining the term FACS.[27] Herzenberg won the Kyoto Prize ... A common variation involves linking the analytical capability of the flow cytometer to a sorting device, to physically separate ... Loken MR (1990). "Immunofluorescence Techniques in Flow Cytometry and Sorting" (2nd ed.). Wiley: 341-53.. ...
Birgens HS (1985). "Lactoferrin in plasma measured by an ELISA technique: evidence that plasma lactoferrin is an indicator of ... A rapid, portable test utilizing microfluidic technology has been developed to enable measurement of lactoferrin levels in ... Analytical Chemistry. 83 (21): 8115-22. doi:10.1021/ac202061v. PMID 21910436. Xavier PL, Chaudhari K, Verma PK, Pal SK, Pradeep ... Karns, Kelly; Herr, Amy E (November 2011). "Human Tear Protein Analysis Enabled by an Alkaline Microfluidic Homogeneous ...
"The Journal of Analytical and Applied Chemistry. 6 (1177): 390-400. Bibcode:1892Natur..46...56A. doi:10.1038/046056c0. p. 398: ... Kamitani, A.; Morishita, S.; Kotaki, H.; Arscott, S. (2011). "Microfabricated microfluidic fuel cells". Sensors and Actuators B ... Kamitani, A.; Morishita, S.; Kotaki, H.; Arscott, S. (2008). "Miniaturized microDMFC using silicon microsystems techniques: ...
Yung CW, Fiering J, Mueller AJ, Ingber DE (May 2009). "Micromagnetic-microfluidic blood cleansing device". Lab on a Chip. 9 (9 ... which is a clinically used technique for the purification of blood and is based on surface adsorption. These advantages are ... analytical tools, physical therapy applications, and drug delivery vehicles. ... "Synthetic ligand-coated magnetic nanoparticles for microfluidic bacterial separation from blood". Nano Letters. 14 (1): 1-5. ...
Other techniques under research include microfluidic separation[62] and combination of immunomagnetic assay and microfluidic ... "Analytical Chemistry. 84 (10): 4292-4299. doi:10.1021/ac2032386. ISSN 0003-2700. PMC 3359653. PMID 22510236.. ... The most common technique is magnetic nanoparticle-based separation (immunomagnetic assay) as used in CellSearch or MACS. ... Epic can also use FISH and other staining techniques to look for abnormalities such as duplications, deletions, and ...
Birgens HS (April 1985). "Lactoferrin in plasma measured by an ELISA technique: evidence that plasma lactoferrin is an ... Karns K, Herr AE (November 2011). "Human tear protein analysis enabled by an alkaline microfluidic homogeneous immunoassay". ... Analytical Chemistry. 83 (21): 8115-22. doi:10.1021/ac202061v. PMID 21910436.. *^ Xavier PL, Chaudhari K, Verma PK, Pal SK, ... portable test utilizing microfluidic technology has been developed to enable measurement of lactoferrin levels in human tear ...
The rastering of the beam across the sample makes STEM suitable for analytical techniques such as Z-contrast annular dark-field ... accomplished by mounting a microfluidic enclosure in the specimen holder.[42][43][44] ... Other STEM techniques[edit]. Specialized sample holders, or modifications to the microscope can allow a number of additional ... The technique was not developed further until the 1970s, when Albert Crewe at the University of Chicago developed the field ...
This technique has provided a record power conversion efficiency (PCE) of 10.7%.[65] The SAM is positioned between ZnO-PbS ... Analytical and Bioanalytical Chemistry. 391 (5): 1609-1618. doi:10.1007/s00216-007-1703-3. PMID 17987281.. ... "A vector-free microfluidic platform for intracellular delivery". Proceedings of the National Academy of Sciences. 110 (6): ... Highly ordered arrays of quantum dots may also be self-assembled by electrochemical techniques. A template is created by ...
Tang T, Badal MY, Ocvirk G, Lee WE, Bader DE, Bekkaoui F, Harrison DJ (February 2002). "Integrated microfluidic electrophoresis ... Cycling probe technology (CPT) is a molecular biological technique for detecting specific DNA sequences. CPT operates under ... Analytical Biochemistry. 432 (2): 106-14. doi:10.1016/j.ab.2012.09.015. PMC 3522425. PMID 23000602.. ... system for analysis of genetic materials using signal amplification methods". Analytical Chemistry. 74 (4): 725-33. doi:10.1021 ...
"Surface Acoustic Wave Nebulization of Peptides As a Microfluidic Interface for Mass Spectrometry". Analytical Chemistry. 82 (10 ... A Flexible Multimode Ambient Ion Generation Technique". Analytical Chemistry. 81 (18): 7788-7794. doi:10.1021/ac9014098. ISSN ... One of the most used plasma-based techniques for ambient ionization is probably Direct analysis in real time (DART), since it ... Gross, Jürgen H. (2013-09-15). "Direct analysis in real time-a critical review on DART-MS". Analytical and Bioanalytical ...
In a digital microfluidic biochip, a group of (adjacent) cells in the microfluidic array can be configured to work as storage, ... Although this technique is very powerful in that many sensors can be created simultaneously, it is currently only feasible for ... Analytical Chemistry 70, pp. 1242-1248, 1998 Alexander, F., Eggert, S., Wiest, J.: Skin-on-a-chip: Transepithelial electrical ... For example, digital microfluidic biochips are under investigation for applications in biomedical fields. ...
The technique itself reduces the use of a larger volume of reagent needed, which inevitably will lower experiment cost. Also, ... Ramakrishnan R, Qin J, Jones RC, Weaver LS (2013). "Integrated Fluidic Circuits (IFCs) for digital PCR". Microfluidic ... Analytical and Bioanalytical Chemistry. 410 (12): 2879-2887. doi:10.1007/s00216-018-0982-1. ISSN 1618-2642. PMC 5996397. PMID ... In 1999, Bert Vogelstein and Kenneth Kinzler coined the term "digital PCR" and showed that the technique could be used to find ...
"Flow control valves for analytical microfluidic chips without mechanical parts based on thermally responsive monolithic ... An example of this deformation is shown in Figure 4. This technique is useful for determining the type of material (brittle, ... A first microfluidic platform technology reported in literature is based on stimuli-responsive gels. To avoid the electrolysis ... Another microfluidic platform is based on ionomeric materials. Pumps made from that material could offer low voltage (battery) ...
This technique was originally proposed by David C. Schwartz and Arvind Ramanathan in 2003. The following is an overview of each ... A microfluidic system for large DNA molecule arrays. Anal. Chem. 76 (2004): 5293-5301. Jo, K., et al. "A Single-Molecule ... "An Integrative Approach for the Optical Sequencing of Single DNA Molecules." Analytical Biochemistry 330.2 (2004): 227-41. ... The advantage of OM over traditional mapping techniques is that it preserves the order of the DNA fragment, whereas the order ...
Becker, H.; Gärtner, C. (2007). "Polymer microfabrication technologies for microfluidic systems". Analytical and Bioanalytical ... This technique is employed often in paint formulations to ensure that they will be evenly spread on a surface. As a result of ... Many techniques can be used to enhance wetting. Surface treatments, such as Corona treatment, plasma treatment and acid etching ... Contact angle Surface tension Sessile drop technique Capillary surface Wulff Construction Marshall, S. J.; Bayne, S. C.; Baier ...
This is particularly useful as it is an area where other biophysical techniques can struggle - for example dynamic light ... Analytical Chemistry. 90 (5): 3284-3290. doi:10.1021/acs.analchem.7b04820. PMID 29313342. "Quantitation of low Tryptophan and ... Microfluidic diffusional sizing (MDS) is a method to measure the size of particles based on the degree to which they diffuse ... Gang, Hongze (2018). "Microfluidic Diffusion Platform for Characterizing the Sizes of Lipid Vesicles and the Thermodynamics of ...
... is a technique used in structural analysis to determine the deflection of Euler-Bernoulli beams. Use of Macaulay's technique is ... It draws upon the logical framework of economics but adds to that the analytical power of mathematics and statistics. ... p. (page needed). Kirby, B. J. (2010). Micro- and Nanoscale Fluid Mechanics: Transport in Microfluidic Devices. Cambridge ... ISBN 978-0-321-50125-7. Edmund Taylor Whittaker (1904). A Treatise on the Analytical Dynamics of Particles and Rigid Bodies. ...
... that the cost of SERS substrates must be reduced in order to become a commonly used analytical chemistry measurement technique ... A SERS-base multiplex protein biomarker detection platform in a microfluidic chip is used to detect several protein biomarkers ... The enhancement factor can be as much as 1010 to 1011, which means the technique may detect single molecules. SERS from ... Research in 2015 on a more powerful extension of the SERS technique called SLIPSERS (Slippery Liquid-Infused Porous SERS) has ...
We have evaluated five bioconjugation chemistries for immobilizing DNA onto silicon substrates for microfluidic biosensing ... Coates J (2000) In: Meyers RA (ed) Encyclopedia of analytical chemistry. Wiley, ChichesterGoogle Scholar ... After immobilization of DNA, a microfluidic channel (1 mm × 2 cm × 45 μm) is aligned over the immobilized DNA, and target DNA ... S1 Schematic of DNA immobilization and microfluidic hybridization assays. Immobilization of DNA in spots defined by a PDMS mask ...
Microfluidic Analytical Techniques* * Motor Neurons / classification * Motor Neurons / physiology* * Motor Neurons / ... A microfluidic device to investigate axon targeting by limited numbers of purified cortical projection neuron subtypes Integr ... We developed a microfluidic system specifically designed to investigate axon targeting of limited numbers of purified CSMN and ...
Microfluidic Analytical Techniques* * Milk, Human / chemistry* * N-Acetylneuraminic Acid / analysis * Oligosaccharides / ... Daily variations in oligosaccharides of human milk determined by microfluidic chips and mass spectrometry J Agric Food Chem. ... Recent advances in analytical tools offer invaluable insights in understanding the specific functions and health benefits these ...
In a previous study a microfluidic brain slice device (microBSD) was developed for microsca ... Understanding and optimizing fluid flows through in vitro microfluidic perfusion systems is essential in mimicking in vivo ... Microfluidic Analytical Techniques*. Microscopy, Fluorescence. Models, Biological*. Perfusion / instrumentation*. Physics*. ... Understanding and optimizing fluid flows through in vitro microfluidic perfusion systems is essential in mimicking in vivo ...
Systems and methods for metering microfluidic volumes are provided. A discrete plug may be separated from a larger volume of ... Fabrication of microfluidic circuits by printing techniques. US6117396 *. 18 Feb 1998. 12 Sep 2000. Orchid Biocomputer, Inc.. ... al., Analytical Chemistry (1997) 69: 2626-2630), or by building a traditional injection molding cavity for plastic devices. ... Such a microfluidic device can be constructed using photopolymerization techniques such as those described in Cumpston, et al ...
Microfluidic Analytical Techniques / instrumentation*, methods. Neoplastic Stem Cells / pathology*. Single-Cell Analysis / ... The microfluidic device and image-recording technique presented here enables a visual characterization of cellular morphology ... 3. Assembly of microfluidic devices and cell culturing (figure 2) In order to culture cells, feature-engraved PDMS stamp is ... In contrast, microfluidic devices permit single cell analysis because of compatibility with modern microscopy and control over ...
Thermal Measurement Techniques in Analytical Microfluidic Devices. Benyamin Davaji1, Chung Hoon Lee1 ... Microfluidic Genipin Deposition Technique for Extended Culture of Micropatterned Vascular Muscular Thin Films. Eric S. Hald1, ... Analyzing Mixing Inhomogeneity in a Microfluidic Device by Microscale Schlieren Technique. Chen-li Sun1, Tzu-hsun Hsiao2 ... Controlled Microfluidic Environment for Dynamic Investigation of Red Blood Cell Aggregation. Rym Mehri1, Catherine Mavriplis1, ...
This manuscript reports on the application of chemometric methods for the development of an optimized microfluidic paper-based ... analytical device (μPAD). As an example, we applied chemometric methods... ... Statistical designs and response surface techniques for the optimization of chromatographic systems. J Chromatogr A. 2007;1158: ... A chemometrics-assisted microfluidic paper-based analytical device was developed as a low-cost and rapid platform for the ...
Solvent Bonding for Fabrication of PMMA and COP Microfluidic Devices, Indoor Experimental Assessment of the Efficiency and ... Fabrication of Gradient Nanopattern by Thermal Nanoimprinting Technique and Screening of the Response of Human Endothelial ... Thermal Measurement Techniques in Analytical Microfluidic Devices, Thermal Imaging to Study Stress Non-invasively in ... Thermal Measurement Techniques in Analytical Microfluidic Devices. Benyamin Davaji1, Chung Hoon Lee1 ...
The silver enhancement reagents may be integrated into the microfluidic assay platform to be released upon sample addition. ... Herein, we demonstrate that adsorptive immobilization via a cationic polymeric interlayer is a competitive and fast technique ... nanocolor microfluidic devices are new promising bioassay platforms, which employ nanoparticle- (NP-) protein conjugates for ... K. N. Han, C. A. Li, and G. H. Seong, "Microfluidic chips for immunoassays," Annual Review of Analytical Chemistry, vol. 6, pp ...
Forensic Analytical Chemistry and Surface Characterization Techniques scheduled on November 19-20, 2020 in November 2020 in ... Microfluidic Paper-Based Electrochemical Biosensor. Ahmad Manbohi, Seyyed Hamid Ahmadi * Designing and Analyzing Sensor and ... Molecular and atomic spectrochemical technique. Electrochemical techniques. Sensors. Surface characterization techniques. Mass ... Forensic Analytical Chemistry and Surface Characterization Techniques. ICFACSCT 2020: 14. International Conference on Forensic ...
Also included are microfluidic devices and integrated systems for performing such assays, including devices utilizing flowable ... in a microfluidic system are provided. The methods include the use of a component-binding moiety specific to the component of ... Analytical system and method. US6074725. 10 Dec 1997. 13 Jun 2000. Caliper Technologies Corp.. Fabrication of microfluidic ... Fabrication of microfluidic circuits by printing techniques. WO1999056954A1. 3 May 1999. 11 Nov 1999. Caliper Technologies ...
Techniques for analyzing an array of compounds utilizing a high throughput microfluidic system are provided. The system can ... and a buffer solution so that the system will alternatingly inject the sample compounds and buffer solution into a microfluidic ... Micro fluidic analytical systems have a number of advantages over conventional chemical or physical laboratory techniques. For ... Analytical detection techniques for droplet microfluidics-A review. Fair et al. 2003. Electrowetting-based on-chip sample ...
The microfluidic reverse affinity-blot device of the present disclosure combines affinity binding for isolation and/or ... microfluidic devices have been proposed to carry out separation techniques in the field of analytical chemistry. Microfluidic ... Any of these techniques, or others, can be used to form microfluidic channels on the substrate of the microfluidic reverse ... The described techniques are merely illustrative and do not limit the techniques that can be used to make microfluidic channels ...
Analytical Techniques for Microscale Analysis  Yen, Gloria Over the years, there has been a rapid rise in the use of ... Cells Incognito: Microfluidic Tools for Detecting and Isolating Cancer Cells  Johnson, Eleanor Sobocinski ... Single-Cell Molecular Profiling of Nucleic Acids in the Microfluidic Self-Digitization Chip  Thompson, Alison Marie ... Studies of Sample Compartmentalization By Microfluidic Methods  Schneider, Thomas (2013-04-17) ...
The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path ... more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic ... An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. ... as an analytical tool. Examples of utilizing microfluidic device as an analytical tool include: * *testing for the presence of ...
Characterization of cellular chemical dynamics using combined microfluidic and Raman techniques. Analytical and Bioanalytical ... Quantitative comparison between microfluidic and microtiter plate formats for cell-based assays. Analytical Chemistry, 80(1), ... Influence of hydrodynamic conditions on quantitative cellular assays in microfluidic systems. Analytical Chemistry, 79(18), ... Analytical Chemistry, 1-7. DOI: 10.1021/ac504695w. *Henry, C., Bolien, D., Ibanescu, B., Bloodworth, S., Harrowven, D. C., ...
The invention also provides microfluidic pumps using two or more microfluidic valves. The valves can be configured to rest in ... The invention provides microfluidic devices having valves disposed therein. The valves can be configured as one-way valves. ... A microfluidic device can be constructed with stencil layers, using techniques described, for example, in co-pending U.S. ... The mold can be constructed using a silicon wafer (see, e.g., Duffy et al., Analytical Chemistry (1998) 70: 4974-4984; ...
BENGGP11: "Microfluidic and Analytical Tools for Synthetic Biology" (an intensive training course for first-year postgraduate ... In addition to general training in microfluidic fabrication techniques and applications, Brian is the course co-ordinator for ... He has extensive experience in the design and fabrication of microfluidic devices in glass and polymers, for applications in ... He is particularly interested in the development of integrated chemo-enzymatic microfluidic reactors for the synthesis of ...
This analytical technique uses an integrated microfluidic chip coupled with a high mass accuracy TOF mass analyzer. The HPLC- ... With these new analytical techniques in hand it will be possible to address important biological questions related to the ... Novel Analytical Tools: Structural Determination of HMO. The basic structure of HMOs includes a lactose core at the reducing ... A unique analytical strategy to rapidly profile oligosaccharides in human milk using HPLC-Chip/time-of-flight (TOF) MS ...
... tools for protein analysis and have been under continuous investigation to develop new methods and to improve the analytical ... compared to conventional microfluidic flow-based immunoassays. Stationary multi-phase microfluidic techniques have been ... Microfluidic communicating vessel chip for expedited and automated immunomagnetic assays Y. Yang and Y. Zeng, Lab Chip, 2018, ... Herein we report a pneumatically gated microfluidic communicating vessel (μCOVE) chip for rapid and sensitive immunomagnetic ...
"Recent advances on optical detection methods and techniques for cell-based microfluidic systems," Chinese Journal of Analytical ... Microfluidic Platforms for Evaluation of Nanobiomaterials: A Review. Venkataraman Giridharan,1 YeoHeung Yun,1 Peter Hajdu,2 ... S. M. Ong, C. Zhang, Y. C. Toh et al., "A gel-free 3D microfluidic cell culture system," Biomaterials, vol. 29, no. 22, pp. ... J.-H. Lee, Y. Gu, H. Wang, and W. Y. Lee, "Microfluidic 3D bone tissue model for high-throughput evaluation of wound-healing ...
Development of E. coli Detection Biosensor Using Analytical Techniques in a Passive Type Microfluidic Chip [PRESENTATION]. ... Applying Mixed Reality Techniques for the Visualization of Programs and Algorithms in a Programming Learning Environment. ... An Enhanced Approach for the Prioritisation in Patent Ductus Arteriosus (PDA) Services Using Data Mining Techniques. Noura ... Scattering Parameters Measurements with the Microwave Transmittance Technique using a Microstrip Patch Antennas, as Non- ...
... inexpensive microfluidic paper-based analytical device (µPAD) for measuring total Cr i ... chromium exposure is also cost and resource intensive because the analysis typically uses sophisticated instrumental techniques ... chromium exposure is also cost and resource intensive because the analysis typically uses sophisticated instrumental techniques ... Chromium-compounds; Analytical-chemistry; Analytical-instruments; Analytical-processes; Metal-compounds; Metallic-compounds; ...
335 Analytical Chemistry. (4). A study of the theories and techniques of modern analytical chemistry. Emphasis is on the kinds ... Emerging technologies such as nanotechnology-enabled biosensors, microfluidic devices and lab-on-chip will also be addressed. ... 336 Advanced Analytical Chemistry. (4). Modern theory and techniques with emphasis on instrumentation. Topics include ... Use of statistical and thermodynamic approaches to develop understanding of analytical and physical techniques and theory. ...
In addition, analytical tools to monitor biologically relevant parameters can be directly integrated on-chip. In this review we ... techniques to mechanically stimulate cells via stretching and fluid flow-induced shear stress; and methods to carry out high- ... Microfluidic approaches for epithelial cell layer culture and characterisation Roland Thuenauer,*ab Enrique Rodriguez-Boulanc ... Microfluidic biochips offer the unique opportunity to establish novel in vitro models of epithelia in which the in vivo ...
Creation of precise analytical techniques for the investigation of intracellular lipid dynamics ... Development of a microfluidic palpation device for single-cell analysis. Research Director:. Noritada Kaji(Associate Professor ... Development of a single-cell nucleic acid analytical technique for understanding biological systems and its applications for ... We would like aim to construct a platform made of fundamental analytical technologies that streamlines data-based single cell ...
Reaction and temperature control for high power microwave-assisted chemistry techniques , Method for plasma etching using ... Methods and apparatus for micro-fluidic analytical chemistry. Improved valve and methods for analytical techniques and systems ... Reaction and temperature control for high power microwave-assisted chemistry techniques. A method is disclosed for carrying out ... Method and apparatus for continuous flow microwave-assisted chemistry techniques. The invention is a method and associated ...
Methods and apparatus for micro-fluidic analytical chemistry. Improved valve and methods for analytical techniques and systems ... Reaction and temperature control for high power microwave-assisted chemistry techniques. A method is disclosed for carrying out ... A method for chemical analysis of fluids utilized in hydrocarbon exploration and production in which microfluidic systems ...
  • The aim of this project is to develop a microfluidics-based system for rapid diagnosis of allergy to drugs by integrating cross-disciplinary expertise of microfluidic bioengineering and medicine. (
  • To analyze the presence and level of certain biomarkers in body fluids, miniaturized immunoassays that make use of microfluidics have become an important analysis technique. (
  • The research on opto-microfluidics provides highly sensitive opto-microfluidic sensors for practical applications with significant advantages of portability, efficiency, sensitivity, versatility, and low cost. (
  • It is obvious that there is no distinct boundary between microfluidics and optofluidics as many techniques and applications are commonly found in these two disciplines. (
  • Important topics in the field of opto-microfluidics include: fabrication of micro- and nano- systems for chemical analysis, manipulation of fluids on microchips, integration of microsensors (chemical, biological, optical, photonic, etc. ) into opto-microfluidic systems, design and modelling of opto-microfluidic devices and systems, and applications of opto-microfluidic systems. (
  • Microfluidics for biotechnology applications require development of inexpensive, high-volume fabrication techniques and reduction of biochemical assays to the chip format. (
  • Researchers have used microfluidics with a flash-flow technique to investigate protein dynamics. (
  • This technique -- known as flow-flash -- has been in use for decades, Dyer said, but to his team's knowledge no one has miniaturized it for use with microfluidics. (
  • Micronit Microfluidics announces the introduction of a new set of tool kits to enable safe microfluidic experiments. (
  • He develops novel analytical science methods using microfluidics, electrochemical sensors and biosensors, and wireless electronics to make portable, wearable monitoring devices. (
  • The technique proposed here offers a low entry barrier for the rapid prototyping of thermoplastic microfluidics, enabling iterative design for laboratories without access to conventional microfabrication equipment. (
  • Spectradyne s technology leverages microfluidics and advanced electrical sensing techniques to measure particle concentration and size with unprecedented accuracy, and requires only a tiny fraction of the sample needed by other methods. (
  • aims to bring together leading academic scientists, researchers and research scholars to exchange and share their experiences and research results on all aspects of Forensic Analytical Chemistry and Surface Characterization Techniques. (
  • Also, high quality research contributions describing original and unpublished results of conceptual, constructive, empirical, experimental, or theoretical work in all areas of Forensic Analytical Chemistry and Surface Characterization Techniques are cordially invited for presentation at the conference. (
  • ICFACSCT 2020 has teamed up with the Special Journal Issue on Forensic Analytical Chemistry and Surface Characterization Techniques . (
  • With a strong cross-disciplinary research background and experience at the interface of chemistry, chemical engineering and life sciences, Xunli's current research interests are primarily centred around the development of microfluidic and Lab-on-a-Chip technologies and their applications in bioengineering. (
  • He has extensive experience in the design and fabrication of microfluidic devices in glass and polymers, for applications in chemistry, chemical engineering and biochemical engineering. (
  • Lockyear earned her Ph.D. in analytical chemistry at the University of Illinois at Urbana-Champaign. (
  • In an Analytical Chemistry paper published online Nov. 11, researchers with the University of Illinois at Urbana-Champaign, Los Alamos National Laboratory in New Mexico and Rensselaer Polytechnic Institute in Troy, N.Y., reported a technique called microfluidic flow-flash that addresses these limitations. (
  • Reprinted with permission of Analytical Chemistry. (
  • Micronit launches the Microfluidic EOF Kit 4515E at Pittcon 2007, the Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy. (
  • What appeals most in those novel synthetic techniques is clear: they bypass the pre-activation steps usually required in traditional cross-coupling chemistry by directly metalating C-H bonds. (
  • Analytical and Bioanalytical Chemistry is published under the leadership of an international team of eminent analytical scientists. (
  • Antje Baeumner is the Director of the Institute of Analytical Chemistry, Chemo- and Biosensors at the University of Regensburg. (
  • She is the president of the International Association of Environmental Analytical Chemistry since June 2018. (
  • Hua Cui is full Professor of Analytical Chemistry at University of Science and Technology of China, China. (
  • She was appointed as a lecturer in 1990, as an associate professor in 1995 and as a professor & head in 2000 in Analytical Division, Department of Chemistry, University of Science and Technology of China. (
  • She received the Award for The National Science Fund for Distinguished Young Scholars from The National Natural Science Foundation of China in 2006, Liang Shuquan Award for Basic Research in Analytical Chemistry from Chinese Chemical Society in 2012, Award for 4nd Ten Outstanding Women Award from The Chinese Academy of Science in 2013 and Outstanding Women Analytical Chemist Award from Analytical Chemistry Division of Chinese Chemical Society in 2015. (
  • He has research experience in the area of biomedical materials and energy materials and has worked on a variety of topics including biocompatibility of metal implants, surface chemistry studies, microfluidic devices and nanoparticle synthesis. (
  • This manuscript reports on the application of chemometric methods for the development of an optimized microfluidic paper-based analytical device (μPAD). (
  • However, a powerful spectroscopy technique such as LSPR should not be limited to the realm of colloidal suspensions, delimiting its capability for producing wearable (bio)sensors and devices. (
  • Microfluidic modulation spectroscopy (MMS) is an infrared spectroscopy technique that is used to characterize the secondary structure of proteins. (
  • However, the lack of automation, repeatability and dynamic range of detection in conventional platforms such as FTIR, have been major limitations which have been addressed with the development of microfluidic modulation spectroscopy. (
  • Circular dichroism spectroscopy (CD) is a technique for the characterization of secondary structure. (
  • Fourier-transform infrared spectroscopy (FTIR) secondary structure deconvolution is also used for multivariate analysis techniques including singular value decomposition, partial least squares, soft independent modeling of class analogy, and neural networks. (
  • Microfluidic modulation spectroscopy is an automated technique that overcomes these challenges of both FTIR and CD for use in biopharmaceutical product characterization. (
  • Comparability and biosimilarity studies often use microfluidic modulation spectroscopy to assess the products for structural differences. (
  • These capabilities make microfluidic modulation spectroscopy a powerful tool in the analysis and development of biosimilars. (
  • Microfluidic modulation spectroscopy can measure previously undetectable changes in protein structural attributes, changes that are critical to drug efficacy and quality. (
  • For reasons mentioned previously, microfluidic modulation spectroscopy provides the sample capacity through 96 well plate operation and technical capabilities to elucidate colloidal and chemical stability, lacking in existing techniques such as size exclusion chromatography (SEC), mass spectrometry and capillary electrophoresis. (
  • The next-gen IR technique Microfluidic Modulation Spectroscopy (MMS) provides high-resolution structural information, critical to understanding the effects of protein misfolding and aggregation on your biotherapeutic drug. (
  • In the Asmis Group , a longstanding experience exists with regard to combining mass spectrometric techniques with laser spectroscopy to characterize the cluster structure, reactivity and dynamics. (
  • To conduct research in these interdisciplinary areas, we have developed core expertise in characterization of electrochemical systems, microfabrication, microfluidic technologies, as well as analytical and computational modeling of transport phenomena, and analytical and material characterization techniques such as various types of spectroscopy and microscopy. (
  • Recently investigators have begun to harness new technology such as microfluidic modulation spectroscopy (MMS) to reveal protein structural changes, as a solid understanding of structure and function is extremely important to the effectiveness of biotherapeutic drugs and scientist today are challenged in gathering a complete understanding with current tools. (
  • Microfluidic Modulation Spectroscopy provides a reliable platform with increased sensitivity and higher resolution for secondary structure analysis of biotherapeutics. (
  • The active fraction was characterized by spectroscopy techniques. (
  • In a previous study a microfluidic brain slice device (microBSD) was developed for microscale electrophysiology investigations. (
  • 8 . The method of claim 1 wherein the microfluidic channel, the first fluid inlet, and the second fluid inlet are defined in a multi-layer microfluidic device made with sandwiched stencil layers. (
  • A chemometrics-assisted microfluidic paper-based analytical device was developed as a low-cost and rapid platform for the determination of uric acid (UA). (
  • In 2009, our group developed a novel microfluidic diagnostic device combining REA technology with an innovative fluidic setup operating by passive capillary force. (
  • The microfluidic reverse affinity-blot device of the present disclosure combines affinity binding for isolation and/or enrichment of protein(s) from a sample, followed by separation/identification thereof. (
  • In general terms, a microfluidic reverse affinity-blot device is a closed system of interconnected components that is comprised of defined points of entry and exit, wherein the interconnected components include a capture region upstream from a protein separation region and subsequent detection region. (
  • 2. The microfluidic device of claim 1, wherein the capture agent comprises antibodies, aptamers, affibodies, avimers or peptides. (
  • 3. The microfluidic device of claim 1, wherein the binding-disruptive reagent comprises detergents, salts, acid, base, or combinations thereof in an aqueous buffered solution, in amounts sufficient to release the captured proteins from the immobilized capture agent. (
  • 4. The microfluidic device of claim 1, comprising two or more loading wells, wherein each loading well is individually fluidically coupled to the capture region. (
  • 5. The microfluidic device of claim 4, wherein at least one of the loading wells is a sample loading well, and at least one other of the loading wells is a capture agent loading well for delivery to the capture region. (
  • 6. The microfluidic device of claim 1, further comprising a collection chamber fluidly connected to the capture region. (
  • 7. The microfluidic device of claim 1, wherein the capture region comprises beads, a functionalized surface, a membrane, a matrix, a monolith, or combination thereof for immobilization of capture agents. (
  • 8. The microfluidic device of claim 1, wherein the capture region comprises immobilized capture agents of two or more capture agent species having different binding specificities for one or more proteins in a sample. (
  • 9. The microfluidic device of claim 1, wherein the separation region comprises a sieving matrix. (
  • 11. The microfluidic device of claim 10, wherein the sieving matrix resolves proteins from 1 kDa to 3000 kDa. (
  • An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. (
  • The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. (
  • The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. (
  • A distinct feature of our device is that it employs the communicating vessel principle as a simple means to generate a fast transient hydrodynamic flow to enable effective flow washing without the need for excessive incubation, which greatly simplifies and expedites the assay workflow, compared to conventional microfluidic flow-based immunoassays. (
  • A microfluidic paper-based analytical device for rapid quantification of particulate chromium. (
  • Assessing chromium exposure is also cost and resource intensive because the analysis typically uses sophisticated instrumental techniques like inductively coupled plasma-mass spectrometry (ICP-MS). Here, we report a novel, simple, inexpensive microfluidic paper-based analytical device (µPAD) for measuring total Cr in airborne particulate matter. (
  • An analytical device including an optically opaque cladding, a sequencing layer including a substrate disposed below the cladding, and a waveguide assembly for receiving optical illumination and introducing illumination into the device. (
  • 3. The analytical device of claim 1 wherein the semitransparent light directing elements comprise metal or semiconductor mirrors, dichroics, prisms, Bragg scatterers, acousto-optic devices, or electro-optic devices. (
  • 4. The analytical device of claim 1 wherein the analytical reaction comprises nucleic acid sequencing, and the fluorescent components comprise fluorescently labeled nucleotides. (
  • 5. The analytical device of claim 1 further comprising a gap in the opaque layer providing for the illumination light to pass through the gap to the light deflecting elements. (
  • 6. The analytical device of claim 5 wherein the illumination light is directed to the light directing elements by a light pipe. (
  • 7. The analytical device of claim 1 wherein the opaque layer comprises a metal. (
  • 8. The analytical device of claim 1 further comprising a detector disposed below the nanoscale apertures for detecting light emitted by the fluorescent components. (
  • 9. The analytical device of claim 8 further comprising integrated directional optical elements for directing the emitted light to one or more sensor elements on the detector. (
  • 10. The analytical device of claim 8 further comprising a filter layer having different filters for multicolor distinction by the detector. (
  • 11. The analytical device of claim 1 wherein the device comprises multiple light pipes that allow illumination light to pass through a layer of nanoscale apertures. (
  • 12. The analytical device of claim 11 wherein the device comprises between about 100,000 and 1 million nanoscale apertures. (
  • The interface array contains electrical pins, electrical contacts or electrical contact pads that directly engage fluid containing reservoirs disposed on a microfluidic device and which are coupled to a power supply. (
  • a sample substrate interface array that comprises a plurality of electrode pins fixedly arranged on the interface array, the electrode pins being electrically coupled to the power supply, and positioned on the interface array to be inserted into a plurality of reservoirs disposed on a surface of a microfluidic device, such that the plurality of electrode pins directly engage fluids disposed in the plurality of reservoirs. (
  • Microfluidic device and surface decoration process for surface capture-based bioassays, US pat. (
  • The first microfluidic device was a miniaturized gas chromatography (GC) system developed at Stanford University in the 1970s [ 1 ]. (
  • In 1985, Unipath Inc. commercialized ClearBlue, a pregnancy test still used today that can be considered the first microfluidic device containing paper and the first microfluidic product to market. (
  • A group of researchers from Osaka University has now developed a nonlinear optical crystal (NLOC) chip, which combines THz waves with a microfluidic device, utilising the close proximity of the THz wave source and the solution of interest in a microchannel. (
  • We focused on two disruptive technologies: a microfluidic cartridge and a new, lens-free imaging device (LensFree), which will enable development of more compact, easier-to-use POCT-type devices," says Damien Isebe, who heads up this highly innovative project at HORIBA Medical. (
  • We present a herringbone-grooved microfluidic device which is covalently functionalized with antibodies against general and cancer exosome membrane biomarkers (CD9 and EpCAM) to isolate exosomes from small volumes of high-grade serous ovarian cancer (HGSOC) serum. (
  • This device and approach can be utilized for a nearly limitless range of downstream exosome analytical and experimental techniques, both on and off-chip. (
  • The development of a low-cost multiparametric platform for enzymatic electrochemical biosensing that can be integrated in a disposable, energy autonomous analytical device is the target of the current work. (
  • PI, Investigation of the inhibiting effect of co-culturing cancer cells and fibroblast cells under the application of alternating electric field in a microfluidic device, Chang Gung Memorial Hospital, CMRPD2G0171, TW$2,281,715, 1 Aug 2017 - 31 July 2019. (
  • Although these chemistries were evaluated for use in microfluidic biosensors, the results provide meaningful insight to a number of nanobiotechnology applications for which microfluidic devices require surface biofunctionalization, for example vascular prostheses and implanted devices. (
  • Evaluation of cancer stem cell migration using compartmentalizing microfluidic devices and live cell imaging. (
  • In contrast, microfluidic devices permit single cell analysis because of compatibility with modern microscopy and control over micro-environment. (
  • We present a method for detailed characterization of BTSC migration using compartmentalizing microfluidic devices. (
  • By combining gradient generators, fluid handling, micro-electrodes and other microfluidic modules, these devices can also be used for drug screening and disease diagnosis. (
  • Recent developments in paper-based microfluidic devices. (
  • Yamada K, Henares TG, Suzuki K, Citterio D. Paper-based inkjet-printed microfluidic analytical devices. (
  • Resonance enhanced absorption (REA) nanocolor microfluidic devices are new promising bioassay platforms, which employ nanoparticle- (NP-) protein conjugates for the immunodetection of medically relevant markers in biologic samples such as blood, urine, and saliva. (
  • Microfluidic devices also called μ TAS (micro total analysis systems) or lab-on-a-chip systems belong to the most promising technologies studied in this context. (
  • Also included are microfluidic devices and. (
  • Also included are microfluidic devices and integrated systems for performing such assays, including devices utilizing flowable or fixed particle sets. (
  • A. D. Elder, S. M. Matthews, J. Swartling, K. Yunus, J. H. Frank, C. M. Brennan, A. C. Fisher, and C. F. Kaminski, "The application of frequency-domain Fluorescence Lifetime Imaging Microscopy as a quantitative analytical tool for microfluidic devices," Opt. (
  • We describe the application of wide-field frequency domain Fluorescence Lifetime Imaging Microscopy (FLIM) to imaging in microfluidic devices. (
  • The sensitivity limits of immunoassays have been enhanced to picomolar concentrations using monoclonal antibodies, new labeling techniques, and devices for signal transduction and acquisition. (
  • Magnetically actuated fluid handling devices for microfluidic applications, US pat. (
  • After a brief introduction of this exciting research field, detailed discussion is focused on different techniques for the fabrication of opto-microfluidic sensors, and various applications of these devices for bioanalysis, chemical detection, and optical measurement. (
  • In order to reveal new applications of various versatile systems, an overview on the development in this exciting field and the state-of-the-art opto-microfluidic devices and systems will be necessary, which will be of interest to researchers in academia and industry. (
  • A combination of microfabrication techniques and molecular biology procedures have the potential to produce powerful, inexpensive, and miniature analytical devices (e.g., microfluidic lab chips), aiding further development of genetic analysis. (
  • We discuss design, fabrication, and testing of plastic microfluidic devices for on-chip genetic sample preparation and DNA microarray detection. (
  • In this study, by leveraging on benefits of additive manufacturing, we have proposed a new method for the fabrication of inertial microfluidic devices. (
  • Using this method, we have fabricated and tested a plethora of existing inertial microfluidic devices, whether in a single or multiplexed manner, such as straight, spiral, serpentine, curvilinear, and contraction-expansion arrays. (
  • Furthermore, the proposed fabrication workflow can be adopted at the production level, enabling large-scale manufacturing of inertial microfluidic devices. (
  • -- Researchers have invented a technique that uses inexpensive paper to make "microfluidic" devices for rapid medical diagnostics and chemical analysis. (
  • Microfluidic devices are also promising analytical systems because of the low sample volumes needed for sample measurement. (
  • Silicone rubber and glass microfluidic devices. (
  • [9] Additionally, advances in microfluidic manufacturing allow the devices to be produced in low-cost plastics [10] and part quality may be verified automatically. (
  • Cellular aging: microfluidic devices such as the "mother machine" allow tracking of thousands of individual cells for many generations until they die. (
  • In this presentation I will describe the development of 3D printed microfluidic devices connected to wireless electronics for transplant organ and patient monitoring. (
  • Recently, microfluidic devices have provided the ability to efficiently capture exosomes based on specific membrane biomarkers, but releasing the captured exosomes intact and label-free for downstream characterization and experimentation remains a challenge. (
  • Her research includes the development of novel nanomaterials such as liposomes, nanofibers and nanoparticles, microfluidic biosensors, sample preparation strategies, and point-of-care devices. (
  • Rapid detection of transition metals in welding fumes using paper-based analytical devices. (
  • The objective of this research was to evaluate a relatively new technology, microfluidic paper-based analytical devices (uPADs), for measuring the metals content in welding fumes. (
  • Paper-based microfluidic devices offer great potential as a low-cost platform to perform chemical and biochemical tests. (
  • In the 90s, efforts arise in the scientific world to automate and integrate one or several laboratory applications in tinny devices by using microfluidic principles and fabrication technologies used mainly in the microelectronics field. (
  • Furthermore, the combination of this technique with electrical impedance measurements, at a single or multiple frequencies, is of great importance to achieve novel reliable diagnostic devices. (
  • Through the development of analytical techniques، microscaled devices have displayed attractive advantages، including ultrasensitive detection and analysis، cost-effectiveness، portability، process integrity، multi-process functionality، and in-situ analysis. (
  • In the last decade، a new generation of analytical devices has emerged based on the cellulose materials - so-called microfluidic paper-based analytical devices (µPADs) - a field that will change the face of the diagnosis of different diseases and sensing of a wide range of biological/chemical/biochemical phenomena. (
  • Using thermoplastic polymers as substrate material is an attractive approach to develop low-cost, disposable microfluidic devices. (
  • Neutral and acidic oligosaccharides in Holstein-Friesian colostrum during the first 3 days of lactation measured by high performance liquid chromatography on a microfluidic chip and time-of-flight mass spectrometry. (
  • Mass spectrometry and nuclear magnetic resonance have already revolutionized the detection and quantification of analytes in proteomic and metabolomic research, yet further enhancement of these analytical technologies and the development of novel methodologies continue to make more and more in-depth investigations possible. (
  • This book, the last in a series of three volumes, touches on topics ranging from X-ray crystallography, NMR, hydrogen-deuterium exchange mass spectrometry, covalent labeling techniques, and ion mobility mass spectrometry. (
  • Fig. S1 Schematic of DNA immobilization and microfluidic hybridization assays. (
  • Based on the development of microfluidic technology, microfluidic systems incorporated with impedance measurement technique, have been reported as a new analytical approach for cell culture-based assays. (
  • These techniques provide an effective and efficient technique for cell culture-based assays. (
  • The microfluidic chip-based acoustic noncontact trapping method earlier developed within the group now provides a flexible platform for performing cell- and particle-based assays in continuous flow microsystems. (
  • Furthermore, rapid technological advances in signal detection and microfluidic handling systems now enable phenotypic assays to be conducted using human cells in two mutually exclusive formats. (
  • In addition to versatile fluidic manipulation of samples on-chip, our microfluidic approach allows confining worm populations close to the Si sensor surface, thus enabling high sensitivity of the assays. (
  • Advances in microfabrication technologies have facilitated the development of microfluidic organ-on-a-chip platforms. (
  • As a result, a huge amount of potential remains intact for other geometries with cross-sections difficult to fabricate with standard microfabrication techniques. (
  • We propose a technology to fabricate nano-electrodes and ultimately biosensors on flexible polymeric-based substrates (cyclo olefin polymer, and polyimide) using standard microfabrication (step and repeat lithography and lift-off) and rapid prototyping techniques (blade cutting). (
  • 13. The instrument of claim 5 , wherein the detector is positioned to receive a signal from a transparent detection zone on a microfluidic substrate when the microfluidic substrate is placed into the substrate attachment region. (
  • The Microfluidic EOF Kit is a user-friendly set-up for lab-on-a-chip experiments requiring electro-osmotic flow (EOF) in combination with conductivity, electrochemical or fluorescence detection. (
  • Both Microfluidic EOF Kits can be used for optical or fluorescence detection. (
  • Moreover, the Microfluidic EOF Kit 4515E enables contactless conductivity detection, for instance with on-chip capillary electrophoresis, facilitated by the high voltage electrodes on the microfluidic chips. (
  • Thirdly, DARPA of the US Department of Defense supported a series of microfluidic research programs in the 1990s after realizing there was a need to develop field-deployable microsystems for the detection of chemical and biological agents that were potential military and terrorist threats. (
  • NAPES has created these advances by bringing together novel technologies incorporating innovative sampling and target pre-concentration, fluid manipulation and precision flow control, microfluidic based sample processing components and highly specific detection methods for determination of bacterial contaminants such as E.coli and chemical pollutants like phosphate and surfactants that can contaminate reservoirs and natural waters. (
  • Such high-sensitivity detection without the need for labelling moieties has great potential for future low-invasivity clinical techniques. (
  • Early and rapid detection of a number of common diseases is expected to be one of the major applications of the technique. (
  • Positron emission tomography (PET) is a highly sensitive whole-body imaging technique based on the detection of radiation from the decay of a radioactive probe ('tracer') administered to the patient. (
  • The basic idea of microfluidic biochips is to integrate assay operations such as detection, as well as sample pre-treatment and sample preparation on one chip. (
  • This eBook introduces new analytical approaches that enable in-line chromatographic detection of exosomes. (
  • The silver enhancement reagents may be integrated into the microfluidic assay platform to be released upon sample addition. (
  • To develop a rapid and sensitive assay based on microfluidic isoelectric focusing, we previously prepared reagent-release capillaries retaining ampholytes by physical adsorption on the inner surface of a short capillary, which were then applied to isoelectric focusing. (
  • Biosensors are a kind of analytical tool that combine recognition biomolecules with a transducer, which transforms (bio)chemical information into a chemical or physical signal. (
  • At Pittcon 2018, taking place in Orlando from 26 February to 1 March 2018, leaders in the field of 'omics' research will present the latest advances and discoveries, and many of the leading manufacturers who provide the tools and technologies facilitating this research will be present to showcase the latest developments in analytical technologies. (
  • PI, Development of a nanofiber/polymer composited microfluidic system and demonstration on impedimetric monitoring of cell proliferation, chemosensitivity, hypoxia, migration, and multi-cellular co-culture in 3D environment, Ministry of Science and Technology, Taiwan, MOST107-2221-E-182-053-MY3, 1 Aug 2018 - 31 July 2021. (
  • PI, Development of a microfluidic system for the investigation of the inhibition mechanisms of tumor spheroids under the combined stimulation of anti-cancer drug and electric field, Chang Gung Memorial Hospital, CMRPD2H0021, TW$3,606,198, 1 Feb 2018 - 31 Jan 2021. (
  • PI, Development of a paper-based 3D co-culture microfluidic system for real-time and non-invasive impedimetric monitoring of cell-cell interaction under various cytokine conditions, Ministry of Science and Technology, MOST104-2221-E-182-014-MY3, TW$2,721,000, 1 Aug 2015 - 31 July 2018. (
  • Understanding and optimizing fluid flows through in vitro microfluidic perfusion systems is essential in mimicking in vivo conditions for biological research. (
  • Systems and methods for metering microfluidic volumes are provided. (
  • Statistical designs and response surface techniques for the optimization of chromatographic systems. (
  • Compared to conventional immunoassays microfluidic systems offer efficient mass transport and a reduced surface to volume ratio. (
  • Drug delivery systems, synthesised with continuous-flow microfluidic reactors for controllable size and shape. (
  • Improved valve and methods for analytical techniques and systems. (
  • A method for chemical analysis of fluids utilized in hydrocarbon exploration and production in which microfluidic systems designed to react to specific. (
  • She co-holds an international patent for an "Apparatus and Method for Trapping Bead Based Reagents within Microfluidic Analysis Systems. (
  • In recent years, increasing effort around the world has been devoted to human health with the development of various novel micro- and nano-technologies, in which the analysis of complex biological systems such as living cells with opto-microfluidic technologies receives significant attention. (
  • This will minimize sample requirements and provide sensitive, disposable analytical systems for high throughput analysis. (
  • As a passive technique, inertial microfluidic systems manipulate cells and particles by taking the advantage of hydrodynamic forces in microchannels with a variety of cross-sections. (
  • He has pioneered the integration of ionic liquids as solvents in droplet microreactors and the application of microfluidic systems to synthesizing biomimetic cell membranes. (
  • Systems in development span the entire process of PET tracer production including dispensing of the radioisotope, synthesis, purification and formulation of the tracer, and analytical testing of the final batch. (
  • Most of these systems incorporate microfluidic components to take advantage of the many scientific and practical benefits of working at small scales. (
  • Typically in microfluidic systems fluids are transported, mixed, separated or otherwise processed. (
  • Microfluidic structures include micropneumatic systems, i.e. microsystems for the handling of off-chip fluids (liquid pumps, gas valves, etc.), and microfluidic structures for the on-chip handling of nanoliter (nl) and picoliter (pl) volumes. (
  • Flow cytometry is the main analytical technology in hematology, but it requires large and complex hardware systems. (
  • These key principles promote interoperability between systems, re-usability of existing components, and the management of complex solutions using software-based techniques. (
  • These techniques were successfully applied and interfaced with relevant biological systems such as primary human myoblasts from dystrophic patients and human embryonic stem cells-derived cardiomyocytes. (
  • The drive to understand the behavior and dynamics of single cells and their connection to population-based properties has prompted the microfluidic community to develop diverse micro-trapping array systems. (
  • The capability to transport cells, bacteria or biomolecules in an aqueous medium has significant potential for these microdevices, also known as micro-Total-Analysis Systems (uTAS) when their application is of analytical nature. (
  • It showed to be a valid method to obtain better reactions efficiency, shorter analysis times, and lower reagents consumption over existing analytical techniques. (
  • In our work, we use cellular force microscopy (CFM), a technique that combines micro-indentation with high-resolution force sensing approaching that of atomic force microscopy, to quantitatively study the mechanical properties of the eggshell of living C. elegans embryos. (
  • Nonetheless, the fabrication of these microchannels is a continuous challenging issue for the microfluidic community, where the most studied channel cross-sections are limited to only rectangular and more recently trapezoidal microchannels. (
  • His research focuses on microfluidic strategies to facilitate material fabrication and biophysical analysis. (
  • PDF] Rapid, cost-efficient fabrication of microfluidic reactors in thermoplastic polymers by combining photolithography and hot embossing. (
  • Fabrication of thermoplastics chips through lamination based techniques. (
  • Abstract We introduce a low-cost, high yield rapid fabrication method for casting COC microfluidic chips that is appropriate for academic labs and small companies. (
  • The microfluidic chip was fabricated in OSTE+ to allow reliable measurement of the dissolved oxygen and the fabrication of high-resolution microstructures down to the &m-range. (
  • He is particularly interested in the development of integrated chemo-enzymatic microfluidic reactors for the synthesis of commercially useful compounds. (
  • We report a cost-efficient and easy to implement process for fabricating microfluidic reactors in thermoplastic materials. (
  • Microfluidic platforms be interfaced with various microscopic, flow cytometry, genomic and proteomic techniques to investigate various molecular and cellular events. (
  • The NAPES project (Next Generation Analytical Platforms for Environmental Sensing, see ) brought together ten partners (three SME Industry, five academic, and two Research Institutes) from Ireland, France, Italy, The Netherlands Spain and the UK to advance the field of environmental sensing by combining parallel 'evolutionary' and 'revolutionary' research activities. (
  • The rapid test is based on a new plastic microfluidic chip where the bacteria are trapped and methods for analysing bacterial growth at single-cell level," says PhD student Özden Baltekin, who performed most of the experimental work. (
  • Multiphysics simulation of a microfluidic perfusion chamber for brain slice physiology. (
  • Use of liquid junction potentials for electrophoresis without applied voltage in a microfluidic channel, US pat. (
  • Capillary electrophoresis is a separation technique that, for instance, has been very successful in the analysis of DNA fragments. (
  • In this respect, passive samplers represent a valid alternative to conventional sampling techniques. (
  • These models can mimic the structural and functional properties of human organs and tissues, hence providing valuable analytical tools for studying fundamental biological processes at in a level of detail not achievable using conventional in vitro models, thus offering potential alternative to animal models. (
  • In conventional isoelectric focusing techniques using microfluidic channels, complicated liquid manipulations are required, which can decrease analytical performance such as the reproducibility of a focusing position. (
  • In this study, reagent-release hydrogels containing an acid or a base solution were developed for isoelectric focusing using short capillaries in place of the acid and base solutions used in conventional microfluidic isoelectric focusing. (
  • Cell-based bioanalysis, using microfluidic and Lab-on-a-Chip methodologies to manipulate and analyse single cells. (
  • Herein we report a pneumatically gated microfluidic communicating vessel (μCOVE) chip for rapid and sensitive immunomagnetic ELISA. (
  • Application of microfluidic gradient chip in the analysis of lung cancer chemotherapy resistance," Journal of Pharmaceutical and Biomedical Analysis , vol. 49, no. 3, pp. 806-810, 2009. (
  • In addition, analytical tools to monitor biologically relevant parameters can be directly integrated on-chip. (
  • The microfluidic chip, shown here encapsulated in purple plastic, is disposable and the mass manufacturing material cost would be less than $1. (
  • Future work will continue to expand the application of chip-based screening and CEC/MS-TOF techniques to other drugs implicated in DFSA. (
  • On the other hand, lab-on-a-chip is concerned with miniaturization and integration of laboratory processes and experiments into single (often microfluidic) chips. (
  • This is a movie of Klebsiella pneumoniae growing in the microfluidic chip imaged in phase contrast. (
  • A schematic drawing of solution measurement by using fabricated terahertz microfluidic chip. (
  • The optical microscope image of the fabricated microfluidic chip is also shown. (
  • In a second study, we present a new microfluidic worm culture system with integrated luminescence-based sensing of the on-chip oxygen concentration for measuring the oxygen uptake of C. elegans worms. (
  • Antibacterial compound was further purified by flash chromatography followed by high-performance liquid chromatography (HPLC) techniques. (
  • Methods of detecting a component of interest, such as a protein, in a microfluidic system are provided. (
  • Immunoassays are workhorse tools for protein analysis and have been under continuous investigation to develop new methods and to improve the analytical performance. (
  • Microfluidic analytical techniques he has developed include methods for measuring the permeability of cell membranes to druglike molecules and techniques for measuring ionic currents through membrane proteins. (
  • Over the past 20 years, great progress has been made by her research group to develop new CL/ECL-based analytical concept and principle and to better understand these analytical methods. (
  • These results have provided novel strategies to address fundamental challenges of CL and ECL as powerful analytical and bioanalytical methods. (
  • Our experiments demonstrated that nanocalorimetry is a direct and highly-sensitive technique that can be combined with other analytical methods to open the way to a more holistic understanding of fundamental biological processes. (
  • Microfluidic biochips offer the unique opportunity to establish novel in vitro models of epithelia in which the in vivo microenvironment of epithelial cells is precisely reconstituted. (
  • The short path lengths of microfluidic flow cells therefore allow the researchers to increase the temporal resolution to where they can measure events occurring on the faster timescales. (
  • Likewise, techniques based on sedimentation are prone to particle adhesion and slower processing time, which increases the non-viability of cells in biological applications. (
  • Techniques for manipulating, separating, and trapping particles and cells are highly desired in today's bioanalytical and biomedical field. (
  • Early in 2012, MIT scientists reported on the development of a postage stamp-sized microchip capable of sorting cells through a technique, known as cell rolling, that mimics a natural mechanism in the body. (
  • In addition, the technique allows living cells to be analysed in a non-destructive way, which has numerous potential benefits in research. (
  • With current technologies, cost of production soars due to the need for specialized radiation safety infrastructure (e.g., hot cells), automated synthesis equipment, analytical testing equipment, and personnel trained in radiochemistry. (
  • This imaging technique provides a wide field of view (≈30mm²) resulting in a counting statistic (a few thousand of cells) in one single acquisition consistent with required hematological performance," explains Sophie Morales, laboratory head at CEA-Leti. (
  • This technique consists on applying amplitude and frequency controlled AC signal to a given microsystem in order to manipulate or sort cells. (
  • Herein, we demonstrate that adsorptive immobilization via a cationic polymeric interlayer is a competitive and fast technique for the binding of the capture protein streptavidin onto planar SiO 2 surfaces such as REA biochips. (
  • Microfluidic chips have proven to be a breakthrough analytical technique that has rendered analysis of proteins a medical routine. (
  • The technique reveals very small conformational differences between different proteins, and provides information on where those differences occur. (
  • FortéBio's Octet instruments are an ideal replacement for ELISA, HPLC, and SPR techniques in quantification of antibodies and recombinant proteins and in testing product potency for lot release. (
  • This volume describes novel and emerging analytical technologies for analysis of proteins with the emphasis on technologies aimed to address characterization "knowledge gaps" and/or improve our ability to measure specified attributes with improved selectivity, sensitivity, resolution, and throughput. (
  • Formulation scientists use a core set of analytical techniques to quantify the colloidal, chemical and conformational stability parameters that define the stability of a biotherapeutic. (
  • Scientists today are challenged in gathering a complete understanding using available techniques. (
  • Spectradyne s CEO Jean-Luc Fraikin described the importance of the deal: This partnership will expand access to the powerful insights that our technology delivers by providing a low barrier to entry for scientists who are new to Microfluidic Resistive Pulse Sensing (MRPS). (
  • The technique provides information on which structural motifs in the protein molecule are changing, providing more guidance when developing stable protein molecules and formulations. (
  • The investigators generally study protein dynamics with a technique involving disassociation of CO from a protein after mixing with an oxygenated buffer, which leads to irreversible binding of oxygen and other reactions. (
  • These changes have been historically difficult to detect, as traditional analytical techniques are not great at detecting small differences in protein structure. (
  • Collaboration between researchers and the manufacturers of analytical equipment allow an ongoing cycle of research and development to provide the analytical methodologies and instrumentation capabilities needed to implement the latest research projects. (
  • We developed a microfluidic system specifically designed to investigate axon targeting of limited numbers of purified CSMN and other projection neurons in culture. (
  • 8 ]. Current research focuses on the optimization of this system as versatile, fast, and sensitive microfluidic POC one-step-immunoassay platform. (
  • An analytical or preparatory system comprised as a base unit, an adapter, and a substrate. (
  • High time resolutions can be achieved using a novel droplet based microfluidic system. (
  • The system goes beyond pre-programmed pipetting operations for common techniques, like reverse pipetting and aliquoting , and allows technicians to easily create, save, and execute pipetting applications from start to finish. (
  • In this report, we describe a novel droplet-based microfluidic system for performing ~50000 single-cell RT-PCR reactions in a single experiment while consuming a minimal amount of reagent. (
  • PI, A paper-based cell culture and subsequent immunoassay microfluidic system for the investigation of cancer cell phosphorylation and signaling pathway, Chang Gung Memorial Hospital, CMRPD3E0101, TW$2,102,043, 1 Jan 2015 - 31 Dec 2016. (
  • Our system is based on microfabricated Si thermopile sensors which are integrated with PDMS microfluidic chips. (
  • A silicon microneedle array with integrated microfluidic channels is presented, which is designed to extract dermal interstitial fluid (ISF) for biochemical analysis. (
  • As one of the premier analytical testing facilities worldwide, specializing in pharmaceutical testing, PTL is adding the Spectradyne nCS1 nanoparticle analysis capability to its comprehensive set of available tools. (
  • The nCS1 instrument will add an additional orthogonal high-resolution technique to our current nanoparticle characterization capabilities. (
  • In particular, the technique of dielectrophoresis (DEP) opened the possibility to manipulate, actuate or transport such biological particles being of great potential in medical diagnostics, environmental control or food processing. (
  • The adapter permits the base unit to be interfaced with a wide variety of different substrates to perform chemical and biological analytical analyses and preparatory procedures. (
  • Despite the historical success of phenotypic screening techniques, target-based screening (often directly measuring the biochemical affinity between chemical compound and the biological target) has predominated compound screening campaigns. (
  • We developed microfluidic microbioreactors that have been used for biological studies involving cell cultures. (
  • Particle Technology Labs is already a trusted provider of high-quality analytical services in many of our target markets, and Spectradyne will provide PTL with increased access to biological markets, including extracellular vesicle research in particular. (
  • This highly adaptable technology is likely to ripple out into many areas of analytical and biochemistry, as well as cell biology, and clinical medicine. (
  • Defining the Next Generation of Analytical and Biophysical Techniques. (
  • During the past decades, plasmonic sensors have been explored extensively due to their ultra-sensitivity and emerged as a new generation of analytical tools. (
  • PTL has used electric sensing zone technology for over 25 years, said William Kopesky, Director of Analytical Services at PTL. (
  • The same measurement techniques are used in patients and in experimental models allowing genuine translational research. (
  • Spectradyne is happy to announce a new partnership with Particle Technology Labs to provide analytical measurement services using its nCS1TM instrument. (
  • Particle Technology Labs is a premier analytical testing facility located in Downers Grove, Illinois, that is ISO certified to provide a broad array of measurement services. (
  • Dr. Weitian Zhao's areas of technical expertise center on inorganic materials synthesis processes and surface analysis using analytical and microscopic techniques. (
  • These microfluidic techniques are grounded on the unique characteristics of microscale flow phenomena and have recently gained prominence as efficient tools for the control and focusing of microbeads. (
  • We developed techniques for obtaining a topological control at the microscale of cell cultures on hydrogels with tunable mechanical properties. (
  • Chemistries were compared in terms of immobilization and hybridization density, stability under microfluidic flow-induced shear stress, and stability after extended storage in aqueous solutions. (
  • Researchers at Oregon State University have developed an improved technique for using magnetic nanoclusters to kill hard-to-reach tumors. (
  • To demonstrate this microfluidic flow-flash technique, the researchers used it to probe the kinetics of CO recombination or oxygen binding to myoglobin after laser-induced dissociation of CO from horse heart myoglobin. (
  • By using patient specific details at the genome, proteome and metabolome level, Prof. Nicholson will explore how the analytical technologies described throughout this article can deliver improved healthcare solutions. (
  • Taken together, this versatile microfluidic toolbox enables novel experimental approaches to characterise epithelial monolayers. (
  • Recent advances in analytical tools offer invaluable insights in understanding the specific functions and health benefits these biomolecules impart to infants. (
  • Microfluidic technology can potentially provide a platform for evaluation of clinical and patient-specific features for cancer therapy, while as an alternative to animal models to reduce the amount of animal tests. (
  • Microfluidic technology has led to the creation of powerful tools for biologists to control the complete cellular environment, leading to new questions and discoveries. (
  • The "fASTest" method is based on sensitive optical and analytical techniques developed to study the behaviour of individual bacteria. (
  • Our research programme encompasses fourteen interdisciplinary research projects with strong synergy effects concerning the molecules and materials involved and the experimental and theoretical techniques that will be applied. (