Microfluidic analytical techniques. Medical search

Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.

The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES.

Silicone polymers which consist of silicon atoms substituted with methyl groups and linked by oxygen atoms. They comprise a series of biocompatible materials used as liquids, gels or solids; as film for artificial membranes, gels for implants, and liquids for drug vehicles; and as antifoaming agents.

The largest country in North America, comprising 10 provinces and three territories. Its capital is Ottawa.

Methodologies used for the isolation, identification, detection, and quantitation of chemical substances.

Microdevices that combine microfluidics technology with electrical and/or mechanical functions for analyzing very small fluid volumes. They consist of microchannels etched into substrates made of silicon, glass, or polymer using processes similar to photolithography. The test fluids in the channels can then interact with different elements such as electrodes, photodetectors, chemical sensors, pumps, and valves.

An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.

A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of PROTEINS; NUCLEIC ACIDS; and CARBOHYDRATES. (Segen, Dictionary of Modern Medicine, 1992)

Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal.

Methods of creating machines and devices.

The preparation and analysis of samples on miniaturized devices.

A highly miniaturized version of ELECTROPHORESIS performed in a microfluidic device.

The systematic identification and quantitation of all the metabolic products of a cell, tissue, organ, or organism under varying conditions. The METABOLOME of a cell or organism is a dynamic collection of metabolites which represent its net response to current conditions.

The spectrometric analysis of fluorescent X-RAYS, i.e. X-rays emitted after bombarding matter with high energy particles such as PROTONS; ELECTRONS; or higher energy X-rays. Identification of ELEMENTS by this technique is based on the specific type of X-rays that are emitted which are characteristic of the specific elements in the material being analyzed. The characteristic X-rays are distinguished and/or quantified by either wavelength dispersive or energy dispersive methods.

Antibodies that are chemically bound to a substrate material which renders their location fixed.

Manufacturing technology for making microscopic devices in the micrometer range (typically 1-100 micrometers), such as integrated circuits or MEMS. The process usually involves replication and parallel fabrication of hundreds or millions of identical structures using various thin film deposition techniques and carried out in environmentally-controlled clean rooms.

Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.

The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.

The design or construction of objects greatly reduced in scale.

A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.

Measurement and evaluation of the components of substances to be taken as FOOD.

The monitoring of the level of toxins, chemical pollutants, microbial contaminants, or other harmful substances in the environment (soil, air, and water), workplace, or in the bodies of people and animals present in that environment.

Determination, by measurement or comparison with a standard, of the correct value of each scale reading on a meter or other measuring instrument; or determination of the settings of a control device that correspond to particular values of voltage, current, frequency or other output.

Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.

Assaying the products of or monitoring various biochemical processes and reactions in an individual cell.

Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).

The evaluation of incidents involving the loss of function of a device. These evaluations are used for a variety of purposes such as to determine the failure rates, the causes of failures, costs of failures, and the reliability and maintainability of devices.

The motion of fluids, especially noncompressible liquids, under the influence of internal and external forces.

The analysis of a chemical substance by inserting a sample into a carrier stream of reagent using a sample injection valve that propels the sample downstream where mixing occurs in a coiled tube, then passes into a flow-through detector and a recorder or other data handling device.

A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry.

A systematic collection of factual data pertaining to health and disease in a human population within a given geographic area.

Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)

A broad family of synthetic organosiloxane polymers containing a repeating silicon-oxygen backbone with organic side groups attached via carbon-silicon bonds. Depending on their structure, they are classified as liquids, gels, and elastomers. (From Merck Index, 12th ed)

Spectrophotometric techniques by which the absorption or emmision spectra of radiation from atoms are produced and analyzed.

The utilization of an electrical current to measure, analyze, or alter chemicals or chemical reactions in solution, cells, or tissues.

Elements of limited time intervals, contributing to particular results or situations.

Drugs intended for human or veterinary use, presented in their finished dosage form. Included here are materials used in the preparation and/or formulation of the finished dosage form.

Polymerized methyl methacrylate monomers which are used as sheets, moulding, extrusion powders, surface coating resins, emulsion polymers, fibers, inks, and films (From International Labor Organization, 1983). This material is also used in tooth implants, bone cements, and hard corneal contact lenses.

Methods for maintaining or growing CELLS in vitro.

Chromatographic techniques in which the mobile phase is a liquid.

The development and use of techniques to study physical phenomena and construct structures in the nanoscale size range or smaller.

A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained.

Alicyclic hydrocarbons in which three or more of the carbon atoms in each molecule are united in a ring structure and each of the ring carbon atoms is joined to two hydrogen atoms or alkyl groups. The simplest members are cyclopropane (C3H6), cyclobutane (C4H8), cyclohexane (C6H12), and derivatives of these such as methylcyclohexane (C6H11CH3). (From Sax, et al., Hawley's Condensed Chemical Dictionary, 11th ed)

Application of statistical procedures to analyze specific observed or assumed facts from a particular study.

Studies in which variables relating to an individual or group of individuals are assessed over a period of time.

The motion of a liquid through a membrane (or plug or capillary) consequent upon the application of an electric field across the membrane. (Oxford Dictionary of Biochemistry and Molecular Biology, 2001)

Statistical formulations or analyses which, when applied to data and found to fit the data, are then used to verify the assumptions and parameters used in the analysis. Examples of statistical models are the linear model, binomial model, polynomial model, two-parameter model, etc.

Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.

The development and use of techniques and equipment to study or perform chemical reactions, with small quantities of materials, frequently less than a milligram or a milliliter.

The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.

Statistical models which describe the relationship between a qualitative dependent variable (that is, one which can take only certain discrete values, such as the presence or absence of a disease) and an independent variable. A common application is in epidemiology for estimating an individual's risk (probability of a disease) as a function of a given risk factor.

Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.

Water-soluble low-molecular-weight polymers of acrylic or methacrylic acid that form solid, insoluble products when mixed with specially prepared ZnO powder. The resulting cement adheres to dental enamel and is also used as a luting agent.

Hard, amorphous, brittle, inorganic, usually transparent, polymerous silicate of basic oxides, usually potassium or sodium. It is used in the form of hard sheets, vessels, tubing, fibers, ceramics, beads, etc.

A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy.

A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both.

A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.

A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.

A plan for collecting and utilizing data so that desired information can be obtained with sufficient precision or so that an hypothesis can be tested properly.

Reducing the SURFACE TENSION at a liquid/solid interface by the application of an electric current across the interface thereby enhancing the WETTABILITY of the surface.

Characteristics or attributes of the outer boundaries of objects, including molecules.

A trace element that constitutes about 27.6% of the earth's crust in the form of SILICON DIOXIDE. It does not occur free in nature. Silicon has the atomic symbol Si, atomic number 14, and atomic weight [28.084; 28.086].

A phenomenon in which the surface of a liquid where it contacts a solid is elevated or depressed, because of the relative attraction of the molecules of the liquid for each other and for those of the solid. (from McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).

Laboratory and other services provided to patients at the bedside. These include diagnostic and laboratory testing using automated information entry.

The integration of epidemiologic, sociological, economic, and other analytic sciences in the study of health services. Health services research is usually concerned with relationships between need, demand, supply, use, and outcome of health services. The aim of the research is evaluation, particularly in terms of structure, process, output, and outcome. (From Last, Dictionary of Epidemiology, 2d ed)

The frequency of different ages or age groups in a given population. The distribution may refer to either how many or what proportion of the group. The population is usually patients with a specific disease but the concept is not restricted to humans and is not restricted to medicine.

Use of various chemical separation and extraction methods, such as SOLID PHASE EXTRACTION; CHROMATOGRAPHY; and SUPERCRITICAL FLUID EXTRACTION; to prepare samples for analytical measurement of components.

The fundamental, structural, and functional units or subunits of living organisms. They are composed of CYTOPLASM containing various ORGANELLES and a CELL MEMBRANE boundary.

A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.

Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.

Polymerized forms of styrene used as a biocompatible material, especially in dentistry. They are thermoplastic and are used as insulators, for injection molding and casting, as sheets, plates, rods, rigid forms and beads.

Electric conductors through which electric currents enter or leave a medium, whether it be an electrolytic solution, solid, molten mass, gas, or vacuum.

Concentration or quantity that is derived from the smallest measure that can be detected with reasonable certainty for a given analytical procedure.

Studies in which the presence or absence of disease or other health-related variables are determined in each member of the study population or in a representative sample at one particular time. This contrasts with LONGITUDINAL STUDIES which are followed over a period of time.

Metabolite fingerprinting: detecting biological features by independent component analysis. (1/1368)

MOTIVATION: Metabolite fingerprinting is a technology for providing information from spectra of total compositions of metabolites. Here, spectra acquisitions by microchip-based nanoflow-direct-infusion QTOF mass spectrometry, a simple and high throughput technique, is tested for its informative power. As a simple test case we are using Arabidopsis thaliana crosses. The question is how metabolite fingerprinting reflects the biological background. In many applications the classical principal component analysis (PCA) is used for detecting relevant information. Here a modern alternative is introduced-the independent component analysis (ICA). Due to its independence condition, ICA is more suitable for our questions than PCA. However, ICA has not been developed for a small number of high-dimensional samples, therefore a strategy is needed to overcome this limitation. RESULTS: To apply ICA successfully it is essential first to reduce the high dimension of the dataset, by using PCA. The number of principal components determines the quality of ICA significantly, therefore we propose a criterion for estimating the optimal dimension automatically. The kurtosis measure is used to order the extracted components to our interest. Applied to our A. thaliana data, ICA detects three relevant factors, two biological and one technical, and clearly outperforms the PCA. (+info)

Microviscoelasticity of the apical cell surface of human umbilical vein endothelial cells (HUVEC) within confluent monolayers. (2/1368)

We studied the local viscoelasticity of the apical membrane of human umbilical vein endothelial cells within confluent layers by magnetic tweezers microrheometry. Magnetic beads are coupled to various integrins by coating with fibronectin or invasin. By analyzing the deflection of beads evoked by various force scenarios we demonstrate that the cell envelope behaves as a linear viscoelastic body if forces up to 2 nN are applied for short times (<20 s) but can respond in an adaptive way if stress pulses are applied longer (>30 s). The time-dependent shear relaxation modulus G(t) exhibits three time regimes: a fast response (t < 0.05 s) where the relaxation modulus G(t) obeys a power law G(t) approximately t(-0.82+/-0.02); a plateau-like behavior (at 0.05 s < t < 0.15 s); and a slow flow-like response which is, however, partially reversible. Strain field mapping experiments with colloidal probes show that local forces induce a strain field exhibiting a range of zeta = 10 +/- 1 microm, but which could only be observed if nonmagnetic beads were coupled to the cell surface by invasin. By application of the theory of elasticity of planar bodies we estimated a surface shear modulus of 2.5 x10(-4) N/m. By assuming a thickness of the actin cortex of approximately 0.5 microm we estimate a Young modulus micro approximately 400 Pa for the apical membrane. The value agrees with a plateau modulus of an entangled or weakly cross-linked actin network of an actin concentration of 100 microM (mesh size 0.2 microm). This result together with our observation of a strong reduction of the shear modulus by the actin destabilizing agent latrunculin A suggests that the shear modulus measured by our technique is determined by the actin cortex. The effect of two ligands inducing actin stress fiber formation and centripetal contraction of cells (associated with the formation of gaps in the confluent cell monolayer) on the viscoelastic responses were studied: histamine and lysophosphatidic acid (LPA). Histamine evoked a dramatic increase of the cell stiffness by >1 order of magnitude within <30 s, which is attributed to a transient rise of the intracellular Ca(2+) level, since DMSO exerted a similar effect. The stiffening is accompanied by a concomitant rounding of the cells as observed by microinterferometry and relaxes partially in the timescale of 5 min, whereas gaps between cells close after approximately 30 min. LPA did not exert a remarkable and reproducible effect other than an occasional very weak transient increase of the shear stiffness, which shows that the gap formation activated by LPA is mediated by a different mechanism than that induced by histamine. (+info)

Formation of droplets of alternating composition in microfluidic channels and applications to indexing of concentrations in droplet-based assays. (3/1368)

For screening the conditions for a reaction by using droplets (or plugs) as microreactors, the composition of the droplets must be indexed. Indexing here refers to measuring the concentration of a solute by addition of a marker, either internal or external. Indexing may be performed by forming droplet pairs, where in each pair the first droplet is used to conduct the reaction, and the second droplet is used to index the composition of the first droplet. This paper characterizes a method for creating droplet pairs by generating alternating droplets, of two sets of aqueous solutions in a flow of immiscible carrier fluid within PDMS and glass microfluidic channels. The paper also demonstrates that the technique can be used to index the composition of the droplets, and this application is illustrated by screening conditions of protein crystallization. The fluid properties required to form the steady flow of the alternating droplets in a microchannel were characterized as a function of the capillary number Ca and water fraction. Four regimes were observed. At the lowest values of Ca, the droplets of the two streams coalesced; at intermediate values of Ca the alternating droplets formed reliably. At even higher values of Ca, shear forces dominated and caused formation of droplets that were smaller than the cross-sectional dimension of the channel; at the highest values of Ca, coflowing laminar streams of the two immiscible fluids formed. In addition to screening of protein crystallization conditions, understanding of the fluid flow in this system may extend this indexing approach to other chemical and biological assays performed on a microfluidic chip. (+info)

Computerized microfluidic cell culture using elastomeric channels and Braille displays. (4/1368)

Computer-controlled microfluidics would advance many types of cellular assays and microscale tissue engineering studies wherever spatiotemporal changes in fluidics need to be defined. However, this goal has been elusive because of the limited availability of integrated, programmable pumps and valves. This paper demonstrates how a refreshable Braille display, with its grid of 320 vertically moving pins, can power integrated pumps and valves through localized deformations of channel networks within elastic silicone rubber. The resulting computerized fluidic control is able to switch among: (i) rapid and efficient mixing between streams, (ii) multiple laminar flows with minimal mixing between streams, and (iii) segmented plug-flow of immiscible fluids within the same channel architecture. The same control method is used to precisely seed cells, compartmentalize them into distinct subpopulations through channel reconfiguration, and culture each cell subpopulation for up to 3 weeks under perfusion. These reliable microscale cell cultures showed gradients of cellular behavior from C2C12 myoblasts along channel lengths, as well as differences in cell density of undifferentiated myoblasts and differentiation patterns, both programmable through different flow rates of serum-containing media. This technology will allow future microscale tissue or cell studies to be more accessible, especially for high-throughput, complex, and long-term experiments. The microfluidic actuation method described is versatile and computer programmable, yet simple, well packaged, and portable enough for personal use. (+info)

Syntheses of 11C- and 18F-labeled carboxylic esters within a hydrodynamically-driven micro-reactor. (5/1368)

Carboxylic esters were successfully labeled with one of two short-lived positron-emitters, carbon-11 or fluorine-18, within a hydrodynamically-driven micro-reactor. The non-radioactive methyl ester was obtained at room temperature; its yield increased with higher substrate concentration and with reduced infusion rate. Radioactive methyl ester was obtained from the reaction of (10 mM) with in 56% decay-corrected radiochemical yield (RCY) at an infusion rate of 10 microL min(-1), and when the infusion rate was reduced to 1 microL min(-1), the RCY increased to 88%. The synthesis of the non-radioactive fluoroethyl ester from and required heating of the micro-reactor on a heating block at 80 degrees C (14-17% RCY), whilst the corresponding radioactive from and was obtained in 10% RCY. The radioactive 'peripheral' benzodiazepine receptor ligand was obtained from the reaction of acid with labeling agent in 45% RCY at an infusion rate of 10 microL min(-1). When the infusion rate was reduced to 1 microL min(-1), the RCY increased to 65%. The results exemplify a new methodology for producing radiotracers for imaging with positron emission tomography that has many potential advantages, including a requirement for small quantities of substrates, enhanced reaction, rapid reaction optimisation and easy product purification. (+info)

Wetting morphologies at microstructured surfaces. (6/1368)

The wetting of microstructured surfaces is studied both experimentally and theoretically. Even relatively simple surface topographies such as grooves with rectangular cross section exhibit a large variety of different wetting morphologies as observed by atomic force microscopy. This polymorphism arises from liquid wedge formation along the groove corners and from contact line pinning along the groove edges. A global morphology diagram is derived that depends only on two system parameters: (i) the aspect ratio of the groove geometry and (ii) The contact angle of the underlying substrate material. For microfluidics, the most interesting shape regimes involve extended liquid filaments, which can grow and shrink in length while their cross section stays essentially constant. Thus, any method by which one can vary the contact angle can be used to switch the length of the filament, as is demonstrated in the context of electrowetting. (+info)

Controlling nonspecific protein adsorption in a plug-based microfluidic system by controlling interfacial chemistry using fluorous-phase surfactants. (7/1368)

Control of surface chemistry and protein adsorption is important for using microfluidic devices for biochemical analysis and high-throughput screening assays. This paper describes the control of protein adsorption at the liquid-liquid interface in a plug-based microfluidic system. The microfluidic system uses multiphase flows of immiscible fluorous and aqueous fluids to form plugs, which are aqueous droplets that are completely surrounded by fluorocarbon oil and do not come into direct contact with the hydrophobic surface of the microchannel. Protein adsorption at the aqueous-fluorous interface was controlled by using surfactants that were soluble in fluorocarbon oil but insoluble in aqueous solutions. Three perfluorinated alkane surfactants capped with different functional groups were used: a carboxylic acid, an alcohol, and a triethylene glycol group that was synthesized from commercially available materials. Using complementary methods of analysis, adsorption was characterized for several proteins (bovine serum albumin (BSA) and fibrinogen), including enzymes (ribonuclease A (RNase A) and alkaline phosphatase). These complementary methods involved characterizing adsorption in microliter-sized droplets by drop tensiometry and in nanoliter plugs by fluorescence microscopy and kinetic measurements of enzyme catalysis. The oligoethylene glycol-capped surfactant prevented protein adsorption in all cases. Adsorption of proteins to the carboxylic acid-capped surfactant in nanoliter plugs could be described by using the Langmuir model and tensiometry results for microliter drops. The microfluidic system was fabricated using rapid prototyping in poly(dimethylsiloxane) (PDMS). Black PDMS microfluidic devices, fabricated by curing a suspension of charcoal in PDMS, were used to measure the changes in fluorescence intensity more sensitively. This system will be useful for microfluidic bioassays, enzymatic kinetics, and protein crystallization, because it does not require surface modification during fabrication to control surface chemistry and protein adsorption. (+info)

High-throughput mouse genotyping using robotics automation. (8/1368)

The use of mouse models is rapidly expanding in biomedical research. This has dictated the need for the rapid genotyping of mutant mouse colonies for more efficient utilization of animal holding space. We have established a high-throughput protocol for mouse genotyping using two robotics workstations: a liquid-handling robot to assemble PCR and a microfluidics electrophoresis robot for PCR product analysis. This dual-robotics setup incurs lower start-up costs than a fully automated system while still minimizing human intervention. Essential to this automation scheme is the construction of a database containing customized scripts for programming the robotics workstations. Using these scripts and the robotics systems, multiple combinations of genotyping reactions can be assembled simultaneously, allowing even complex genotyping data to be generated rapidly with consistency and accuracy. A detailed protocol, database, scripts, and additional background information are available at http://dir.nhlbi.nih.gov/labs/ldb-chd/autogene/. (+info)

Although MS is an attractive analytical technique for distinguishing the products of reactions accomplished through DMF, it ... Luk VN, Wheeler AR (June 2009). "A digital microfluidic approach to proteomic sample processing". Analytical Chemistry. 81 (11 ... The composition and purity of molecules synthesized by DMF are often determined utilizing classic analytical techniques. ... Ng AH, Uddayasankar U, Wheeler AR (June 2010). "Immunoassays in microfluidic systems. Analytical and bioanalytical chemistry". ...

https://en.wikipedia.org/wiki/Digital_microfluidics

There is no common analytical approach for quantitation due to the constraints of traditional techniques (e.g. the limited ... Microfluidic modulation spectroscopy is an automated technique that overcomes these challenges of both FTIR and CD for use in ... Microfluidic modulation spectroscopy (MMS) is an infrared spectroscopy technique that is used to characterize the secondary ... Formulation scientists use a core set of analytical techniques to quantify the colloidal, chemical and conformational stability ...

https://en.wikipedia.org/wiki/Microfluidic_modulation_spectroscopy

Microfluidic Paper-based Analytical Devices". Analytical Chemistry. 82 (1): 3-10. doi:10.1021/ac9013989. PMID 20000334. Osborn ... Therefore, microfluidic devices require alternative flow control techniques, a number of which are currently popular: One ... Foudeh, Amir M.; Didar, Fohid Fatanat; Veres, Teodor; Tabrizian, Maryam (2012). "Microfluidic designs and techniques using lab- ... Optical detection includes fluorescence-based techniques, chemiluminescence-based techniques, and surface plasmon resonance ( ...

https://en.wikipedia.org/wiki/Bio-MEMS

... microfluidic analytical techniques MeSH E05.196.630.465.340 - electrophoresis, microchip MeSH E05.196.630.570 - microarray ... embryo culture techniques MeSH E05.200.249.484 - organ culture techniques MeSH E05.200.249.617 - tissue culture techniques MeSH ... cell culture techniques MeSH E05.200.249.374 - coculture techniques MeSH E05.200.249.437 - diffusion chambers, culture MeSH ... fluorescent antibody technique MeSH E05.200.750.551.512.240.300 - fluorescent antibody technique, direct MeSH E05.200.750.551. ...

https://en.wikipedia.org/wiki/List_of_MeSH_codes_(E05)

Paper-based microfluidic devices are often referred to as microfluidic paper-based analytical devices (µPADs) and can detect ... Microfluidic techniques such as droplet microfluidics, paper microfluidics, and lab-on-a-chip are used in the realm of food ... Overall, microfluidic techniques have a strong potential for personalized cancer treatment and bringing in new methods for ... Research in nutrition, food processing, and food safety benefit from microfluidic technique because experiments can be done ...

https://en.wikipedia.org/wiki/Microfluidics

Kung, Chia-Te; Hou, Chih-Yao; Wang, Yao-Nan; Fu, Lung-Ming (2019-12-12). "Microfluidic paper-based analytical devices for ... and has since been used for techniques such as paper chromatography and lateral flow assays. However, it was only identified as ... Akyazi, Tugce; Basabe-Desmonts, Lourdes; Benito-Lopez, Fernando (2018-02-25). "Review on microfluidic paper-based analytical ... "Advances in Microfluidic Paper-Based Analytical Devices for Food and Water Analysis". Micromachines. 7 (5): 86. doi:10.3390/ ...

https://en.wikipedia.org/wiki/Paper-based_biosensor

... is a German analytical chemist who is a professor in both the School of Molecular Sciences and Center for Applied ... Her research considers microfluidic platforms and their use in analysis. She was awarded the 2020 Advancing Electrokinetic ... Ros, Alexandra (2000). New protein separation and analysis techniques. Erscheinungsort nicht ermittelbar: Verlag nicht ... "New microfluidic device minimizes loss of high value samples". EurekAlert!. Retrieved 2021-08-22. "ASU School of Molecular ...

https://en.wikipedia.org/wiki/Alexandra_Ros

This technique is relatively cheap and can be used to make nearly any architecture used in microfluidic experiments. Depending ... Analytical Chemistry. 73 (6): 1240-1246. doi:10.1021/ac001132d. Tice JD, Song H, Lyon AD, Ismagilov RF (2003). "Formation of ... Depending upon the geometry of the microfluidic device as well as the flow rates used, droplets can also be formed using a flow ... The versatility in microfluidic device design and experimental execution combined with the unique size advantages of ...

https://en.wikipedia.org/wiki/Microfluidics_in_chemical_biology

Functionalization of porous surfaces have seen great success with high temperature photografting techniques. In microfluidic ... In industrial corona and plasma processes, cost-efficient and rapid analytical methods are required for confirming adequate ... This grafting technique allows for excellent control over the peptide composition as the bonded chain can be washed without ... These techniques provide characterization at surface depths of 1-10 nanometers, approximately the range of oxidation in plasma ...

https://en.wikipedia.org/wiki/Polymeric_surface

Microfluidic Paper-Based Analytical Devices". Analytical Chemistry. 82 (1): 3-10. doi:10.1021/ac9013989. ISSN 0003-2700. PMID ... Due to this polymerization technique, the paper microfluidic device could be folded using origami, allowing for both horizontal ... Recently, a paper was employed in the production of more complicated microfluidic analytical devices, called lab-on-a-chip (LOC ... Liu, Shuopeng; Su, Wenqiong; Ding, Xianting (2016-12-08). "A Review on Microfluidic Paper-Based Analytical Devices for Glucose ...

https://en.wikipedia.org/wiki/Paper-based_microfluidics

Microfluidic devices can combine several analytical steps into one device. This technology has been coined by some as the "lab ... Microfluidic whole genome haplotyping is a technique for the physical separation of individual chromosomes from a metaphase ... Like with the microfluidic technique, specialized amplification platforms are necessary to address the problem of a small ... Most molecular biology techniques for haplotyping can accurately determine haplotypes of only a limited region of the genome. ...

https://en.wikipedia.org/wiki/Microfluidic_whole_genome_haplotyping

... microfluidic chip with optical tweezing in order to isolate bacteria with altered phenotype directly from the analytical matrix ... Optical techniques such as phase contrast microscopy in combination with single-cell analysis are another powerful method to ... Clinical resistance is shown through the failure of many therapeutic techniques where the bacteria that are normally ...

https://en.wikipedia.org/wiki/Antimicrobial_resistance

Mass spectrometry techniques have become important analytical tools for proteomic and metabolomic analysis of single cells. ... The development of hydrodynamic-based microfluidic biochips has been increasing over the years. In this technique, the cells or ... The development of hydrodynamic-based microfluidic biochips has been increasing over the years. In this technique, the cells ... Secondary ion mass spectrometry (SIMS) is a technique similar to DESI, but while DESI is an ambient ionization technique, SIMS ...

https://en.wikipedia.org/wiki/Single-cell_analysis

The centrifugal micro-fluidic biochip or centrifugal micro-fluidic biodisk is a type of lab-on-a-chip technology, also known as ... The micromachining techniques, including patterning, photolithography, and etching should all be used as long as the design is ... Morais, Sergi (2008). "Analytical prospect of compact disk technology in immunosensing". Anal Bioanal Chem. 391 (8): 2837-2844 ... Once the centrifugal micro-fluidic biochip is developed well enough to be manufactured on a large scale, it will cause a wide ...

https://en.wikipedia.org/wiki/Centrifugal_micro-fluidic_biochip

2006). Microfluidic Techniques: Reviews and Protocols. Methods in Molecular Biology. Humana Press. ISBN 9781588295170. " ... 2018 American Chemical Society Division of Analytical Chemistry Award in Electrochemistry 2018 American Association for the ... Young Investigator Award 2006 United States Department of Defense Okaloosa Award 2006 Springer Nature Microfluidic Techniques ... "2018 Division Award Winners - ACS Division of Analytical Chemistry". acsanalytical.org. 10 February 2018. Retrieved 2019-04-13 ...

https://en.wikipedia.org/wiki/Shelley_D._Minteer

GC-MS is a technique utilized in many analytical laboratories and is a very effective and adaptable analytical tool. Liquid ... Sharif KM, Chin ST, Kulsing C, Marriott PJ (September 2016). "The microfluidic Deans switch: 50 years of progress, innovation ... Different combinations of one dimensional GC and LC produced the analytical chromatographic technique that is known as two- ... Comprehensive two-dimensional gas chromatography is an analytical technique that separates and analyzes complex mixtures. It ...

https://en.wikipedia.org/wiki/Two-dimensional_chromatography

OI Analytical, in its gas diffusion amperometric total cyanide method, uses a segmented flow injection analysis technique that ... Microminiaturized chromatography is carried out on microcolumns that are automatically renewed by microfluidic manipulations. ... Alpkem was purchased by Perstorp Group, and then later by OI Analytical in College Station Texas. OI Analytical manufactures ... The Bran+Luebbe CFA business was bought by SEAL Analytical in 2006 and they continue to manufacture, sell and support the ...

https://en.wikipedia.org/wiki/AutoAnalyzer

... micrototal analytical systems or lab-on-a-chip structures. For instance, NCAMs, when incorporated into microfluidic devices, ... Standard photolithography, bulk or surface micromachining, replication techniques (embossing, printing, casting and injection ... One of the more promising areas of nanofluidics is its potential for integration into microfluidic systems, i.e. ... CS1: long volume value, Nanotechnology, Fluid dynamics, Analytical chemistry, Surface science, Materials science). ...

https://en.wikipedia.org/wiki/Nanofluidics

Hydrophobic Interaction Chromatography (HIC) is a purification and analytical technique that separates analytes, such as ... February 2007). "Gravity-driven microfluidic particle sorting device with hydrodynamic separation amplification". Analytical ... simulated moving bed technique was proposed. In the simulated moving bed technique instead of moving the bed, the sample inlet ... He developed the technique and coined the term chromatography in the first decade of the 20th century, primarily for the ...

https://en.wikipedia.org/wiki/Chromatography

Due to the ATR geometry and the resulting evanescent wave, it is possible with this technique to study transport phenomena and ... Carter, Catherine F. (2010). "ReactIR Flow Cell: A New Analytical Tool for Continuous Flow Chemical Processing". Organic ... ATR-IR has been applied to microfluidic flows of aqueous solutions by engineering microreactors with built-in apertures for the ... Attenuated total reflection (ATR) is a sampling technique used in conjunction with infrared spectroscopy which enables samples ...

https://en.wikipedia.org/wiki/Attenuated_total_reflectance

Elbow strength pipetting Technique: Elbow flexion or abduction. Arm strength diminishes as elbow posture is deviated from a 90 ... Ainla, Alar; Jansson, Erik T.; Stepanyants, Natalia; Orwar, Owe; Jesorka, Aldo (June 2010). "A Microfluidic Pipette for Single- ... Cell Pharmacology". Analytical Chemistry. 82 (11): 4529-4536. doi:10.1021/ac100480f. PMID 20443547. Aimee Cunningham (2007-04- ... Winged elbow pipetting Technique: elevated, "winged elbow". The average human arm weighs approximately 6% of the total body ...

https://en.wikipedia.org/wiki/Pipette

A key theme of deMello's research has been the development of ultra-high sensitivity detection methods for use in microfluidic ... More recently, his group have introduced the technique of stroboscopic imaging flow cytometry, which allows for high resolution ... and in 1997 moved back to his alma mater to take up the AstraZeneca Lectureship in Analytical Sciences at Imperial College ... His group has pioneered the use of microfluidic systems for small molecule chemistry and nanomaterial synthesis, and in recent ...

https://en.wikipedia.org/wiki/Andrew_deMello

ISBN 1-57444-572-3. Minteer, Shelley D. (2006). Microfluidic Techniques: Reviews and Protocols. Humana Press. ISBN 1-59259-997- ... This device represents a key technology to fields such as chemical industry, pharmaceutical industry, analytical chemistry, ... ISBN 978-0-387-28597-9. Li, Paul C. H. (2005). Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery. ... ISBN 978-1-58053-972-2. Hardt, Steffen & Schönfeld, Friedhelm (2007). Microfluidic Technologies for Miniaturized Analysis ...

https://en.wikipedia.org/wiki/Micromixer

"Catherine Costello". The Analytical Scientist. Retrieved 2021-11-07. "John B. Fenn Distinguished Contribution". www.asms.org. ... Her research involves structural characterization of biopolymers using mass spectrometry-based techniques, such as liquid ... microfluidic capillary electrophoresis-mass spectrometry, and ion mobility spectrometry-mass spectrometry. She was one of the ... Award 2019 inaugural winner of the US Human Proteome Organization Lifetime Achievement in Proteomics Award 2019 Analytical ...

https://en.wikipedia.org/wiki/Catherine_E._Costello

Common techniques include: Fluorescence activated cell sorting (FACS) Microfluidic devices Combining FACS with scRNA-seq has ... they also presented new computational and analytical challenges. Bioinformaticians can use techniques from bulk RNA-seq for ... Common techniques for measuring expression are quantitative PCR or RNA-seq. There are several methods available to isolate and ... Moreover, combining microfluidic devices with scRNA-seq has been optimized in 10x Genomics protocols. To measure the level of ...

https://en.wikipedia.org/wiki/Single-cell_transcriptomics

Meyvantsson I, Beebe DJ (2008-06-13). "Cell culture models in microfluidic systems". Annual Review of Analytical Chemistry. 1 ( ... This technique has demonstrated a stark difference in the sensitivity of the peripheral terminals compared to the neuronal cell ... Meyvantsson, Ivar; Beebe, David J. (2008). "Cell culture models in microfluidic systems". Annual Review of Analytical Chemistry ... Since the advent of poly(dimethylsiloxane) (PDMS) microfluidic device fabrication through soft lithography microfluidic devices ...

https://en.wikipedia.org/wiki/Microfluidic_cell_culture

Tang T, Badal MY, Ocvirk G, Lee WE, Bader DE, Bekkaoui F, Harrison DJ (February 2002). "Integrated microfluidic electrophoresis ... Cycling probe technology (CPT) is a molecular biological technique for detecting specific DNA sequences. CPT operates under ... Analytical Biochemistry. 432 (2): 106-14. doi:10.1016/j.ab.2012.09.015. PMC 3522425. PMID 23000602. (Molecular biology, ... system for analysis of genetic materials using signal amplification methods". Analytical Chemistry. 74 (4): 725-33. doi:10.1021 ...

https://en.wikipedia.org/wiki/Cycling_probe_technology

Research on microfluidic found its advantages in DNA analysis, lab-on-a-chip, and micro-TAS. Devices in a microfluidic system ... Advances in nanofabrication techniques and concerns about energy shortage make people interested in this idea. The main ... The future of nanofluidic systems will be focused on several areas such as analytical chemistry and biochemistry, liquid ... Integration of these microfluidic devices enables sorting, transporting, and mixing of substances within fluids. However, the ...

https://en.wikipedia.org/wiki/Nanofluidic_circuitry

Despite sharing some similarities in concept, there are significant differences between the two techniques. Micro-fluidic SPR ... Analytical Biochemistry. 508: 78-96. doi:10.1016/j.ab.2016.06.024. PMID 27365220. (Biosensors, Molecular biology techniques). ... Syahir A, Usui K, Tomizaki KY, Kajikawa K, Mihara H (April 2015). "Label and Label-Free Detection Techniques for Protein ... Thus, BLI finds significant use in viscous media such as glycerol, where other techniques may struggle. Bio-layer ...

https://en.wikipedia.org/wiki/Bio-layer_interferometry

Many techniques exist to detect DNA, which is usually a means to detect organisms that have that particular DNA. DNA sequences ... A biosensor is an analytical device, used for the detection of a chemical substance, that combines a biological component with ... The opposite side is then contacted with a microfluidic flow system. The contact with the flow system creates channels across ... These techniques are mainly used in agriculture, food technology and biomedicine. In medical applications biosensors are ...

https://en.wikipedia.org/wiki/Biosensor

Although this technique was first thought of as a way to accurately define a standard unit of current, it turned out to be more ... In recent years, attention has been drawn to using SAWs to drive microfluidic actuation and a variety of other processes. Owing ... Trends in Analytical Chemistry. 29 (2): 141-157. doi:10.1016/j.trac.2009.11.002. Sesen, Muhsincan; Alan, Tuncay; Neild, Adrian ... This technique can be used as an alternative to microchannels and microvalves for manipulation of substrates, allowing for an ...

https://en.wikipedia.org/wiki/Surface_acoustic_wave

"Surface Acoustic Wave Nebulization of Peptides As a Microfluidic Interface for Mass Spectrometry". Analytical Chemistry. 82 (10 ... A Flexible Multimode Ambient Ion Generation Technique". Analytical Chemistry. 81 (18): 7788-7794. doi:10.1021/ac9014098. ISSN ... One of the most used plasma-based techniques for ambient ionization is probably Direct analysis in real time (DART), since it ... Gross, Jürgen H. (2013-09-15). "Direct analysis in real time-a critical review on DART-MS". Analytical and Bioanalytical ...

https://en.wikipedia.org/wiki/Ambient_ionization

In a digital microfluidic biochip, a group of (adjacent) cells in the microfluidic array can be configured to work as storage, ... Although this technique is very powerful in that many sensors can be created simultaneously, it is currently only feasible for ... Analytical Chemistry 70, pp. 1242-1248, 1998 Alexander, F., Eggert, S., Wiest, J.: Skin-on-a-chip: Transepithelial electrical ... For example, digital microfluidic biochips are under investigation for applications in biomedical fields. ...

https://en.wikipedia.org/wiki/Biochip

"Flow control valves for analytical microfluidic chips without mechanical parts based on thermally responsive monolithic ... An example of this deformation is shown in Figure 4. This technique is useful for determining the type of material (brittle, ... A first microfluidic platform technology reported in literature is based on stimuli-responsive gels. To avoid the electrolysis ... Another microfluidic platform is based on ionomeric materials. Pumps made from that material could offer low voltage (battery) ...

https://en.wikipedia.org/wiki/Electroactive_polymer

The technique itself reduces the use of a larger volume of reagent needed, which inevitably will lower experiment cost. Also, ... Ramakrishnan R, Qin J, Jones RC, Weaver LS (2013). "Integrated Fluidic Circuits (IFCs) for digital PCR". Microfluidic ... Analytical and Bioanalytical Chemistry. 410 (12): 2879-2887. doi:10.1007/s00216-018-0982-1. PMC 5996397. PMID 29556737. Baker M ... In 1999, Bert Vogelstein and Kenneth Kinzler coined the term "digital PCR" and showed that the technique could be used to find ...

https://en.wikipedia.org/wiki/Digital_polymerase_chain_reaction

This technique was originally proposed by David C. Schwartz and Arvind Ramanathan in 2003. The following is an overview of each ... A microfluidic system for large DNA molecule arrays. Anal. Chem. 76 (2004): 5293-5301. Jo, K., et al. "A Single-Molecule ... "An Integrative Approach for the Optical Sequencing of Single DNA Molecules." Analytical Biochemistry 330.2 (2004): 227-41. ... The advantage of OM over traditional mapping techniques is that it preserves the order of the DNA fragment, whereas the order ...

https://en.wikipedia.org/wiki/Optical_mapping

Kamitani, A.; Morishita, S.; Kotaki, H.; Arscott, S. (2011). "Microfabricated microfluidic fuel cells". Sensors and Actuators B ... Kamitani, A.; Morishita, S.; Kotaki, H.; Arscott, S. (2008). "Miniaturized microDMFC using silicon microsystems techniques: ... The Journal of Analytical and Applied Chemistry. 6 (1177): 390-400. Bibcode:1892Natur..46...56A. doi:10.1038/046056c0. p. 398: ...

https://en.wikipedia.org/wiki/Methanol

Becker, H.; Gärtner, C. (2007). "Polymer microfabrication technologies for microfluidic systems". Analytical and Bioanalytical ... This technique is employed often in paint formulations to ensure that they will be evenly spread on a surface. As a result of ... Many techniques can be used to enhance wetting. Surface treatments, such as corona treatment, plasma treatment and acid etching ... Contact angle Surface tension Sessile drop technique Capillary surface Wulff Construction Marshall, S. J.; Bayne, S. C.; Baier ...

https://en.wikipedia.org/wiki/Surface_energy

"Optical and digital microscopic imaging techniques and applications in pathology". Analytical Cellular Pathology. 34 (1-2): 5- ... "A Camouflaged Film Imitating the Chameleon Skin with Color-Changing Microfluidic Systems Based on the Color Information ... Many fish, such as the marine hatchetfish, use a combination of camouflage techniques to achieve these appearances. Silvering, ...

https://en.wikipedia.org/wiki/Bio-inspired_photonics

This is particularly useful as it is an area where other biophysical techniques can struggle - for example dynamic light ... Analytical Chemistry. 90 (5): 3284-3290. doi:10.1021/acs.analchem.7b04820. PMID 29313342. "Quantitation of low Tryptophan and ... Microfluidic diffusional sizing (MDS) is a method to measure the size of particles based on the degree to which they diffuse ... Gang, Hongze (2018). "Microfluidic Diffusion Platform for Characterizing the Sizes of Lipid Vesicles and the Thermodynamics of ...

https://en.wikipedia.org/wiki/Microfluidic_diffusional_sizing

... that the cost of SERS substrates must be reduced in order to become a commonly used analytical chemistry measurement technique ... A SERS-base multiplex protein biomarker detection platform in a microfluidic chip is used to detect several protein biomarkers ... The enhancement factor can be as much as 1010 to 1011, which means the technique may detect single molecules. SERS from ... Research in 2015 on a more powerful extension of the SERS technique called SLIPSERS (Slippery Liquid-Infused Porous SERS) has ...

https://en.wikipedia.org/wiki/Surface-enhanced_Raman_spectroscopy

Analytical Electrochemistry (1st, 2nd and 3rd Eds, 1994, 1999, 2006) Biosensors and Chemical Sensors Biosensors for Direct ... microfluidic ("Lab-on-a-Chip") devices, and remote sensors for environmental and security monitoring. Wang led a team that ... Monitoring of Environmental Pollutants in Field Electrochemistry of Nucleic Acids and Proteins Electrochemical Techniques in ... Inaugural IUPAC Analytical Chemistry Medal, 2021 IEEE Sensors Achievement Award, 2021. Ralph Adams Award in Bioanalytical ...

https://en.wikipedia.org/wiki/Joseph_Wang

... is used to permanently bond PDMS Microfluidic chips with glass slides or PDMS slabs to create water-tight ... Biro, David A.; Pleizier, Gerald; Deslandes, Yves (1993). "Application of the microbond technique. IV. Improved fiber-matrix ... Sun, Tong; Blanchard, Pierre-Yves; Mirkin, Michael V. (2015-04-21). "Cleaning Nanoelectrodes with Air Plasma". Analytical ... The most widely used material for microfluidic device prototyping is polydimethylsiloxane (PDMS), for its rapid development and ...

https://en.wikipedia.org/wiki/Plasma_cleaning

Birgens HS (April 1985). "Lactoferrin in plasma measured by an ELISA technique: evidence that plasma lactoferrin is an ... A rapid, portable test utilizing microfluidic technology has been developed to enable measurement of lactoferrin levels in ... Analytical Chemistry. 83 (21): 8115-22. doi:10.1021/ac202061v. PMID 21910436. Xavier PL, Chaudhari K, Verma PK, Pal SK, Pradeep ... Karns K, Herr AE (November 2011). "Human tear protein analysis enabled by an alkaline microfluidic homogeneous immunoassay". ...

https://en.wikipedia.org/wiki/Lactoferrin

doi:10.1016/0924-4247(93)80213-Z. van der Wijngaart (2001). "A valve-less diffuser micropump for microfluidic analytical ... Large compression ratio is achieved thanks to special patented technique of piezo mounting, when electrical voltage is applied ... Within analytical systems, the micropump can be for lab-on-chip applications, HPLC and Gas Chromatography systems etc. For the ... Within the microfluidic world, physical laws change their appearance. As an example, volumetric forces, such as weight or ...

https://en.wikipedia.org/wiki/Micropump

The application of this preparatory technique gives rise to tunable pore sizes. Temperature gradient elutions can be used to ... The inner surface of the microfluidic channels is composed of polyethylene terephthalate, to which the PEG-b/pNIPAAm beads ... Irene Tan, Farnoosh Roohi, Maria-Magdalena Titirici, Thermoresponsive polymers in liquid chromatography, Analytical Methods, ... Noah Malmstadt; Paul Yager; Allan S. Hoffman; Patrick S. Stayton (2003). "A smart microfluidic affinity chromatography matrix ...

https://en.wikipedia.org/wiki/Thermoresponsive_polymers_in_chromatography

... techniques known as microfluidic resistive pulse sensing as well as one commercial venture which sells a technique it terms ... The Coulter principle is most commonly employed in a Coulter counter, which is an analytical instrument designed for a specific ... The technique has been used to diagnose a variety of diseases, and is the standard method for obtaining red blood cell counts ( ... The Coulter principle and the Coulter counter that is based on it is the commercial term for the technique known as resistive ...

https://en.wikipedia.org/wiki/Coulter_counter

Liver-on-a-chip devices utilize microfluidic techniques to simulate the hepatic system by imitating complex hepatic lobules ... Analytical Chemistry. 78 (13): 4291-8. doi:10.1021/ac051856v. PMID 16808435. Kang YB, Sodunke TR, Lamontagne J, Cirillo J, ... but this technique results in diffusion limitations of nutrients and dissolved gases. Because microfluidic systems introduce ... An organ-based microfluidic platform has been developed as a lab-on-a-chip onto which a fragile blood vessel can be fixed, ...

https://en.wikipedia.org/wiki/Organ-on-a-chip

Moon, H.; Wheeler, A.R.; Garrell, R.L.; Loo, J.A.; Kim, C.-J., "An Integrated Digital Microfluidic Chip for Multiplexed ... Reprinted in Instrumentation in Analytical Chemistry; L. Voress, Ed.; American Chemical Society: Washington, DC, 1992. Also ... where she worked with Samuel Krimm to develop surface-enhanced Raman spectroscopy as a technique for characterizing adsorption ... Son, S.U.; Garrell, R.L. "Transport of Live Yeast and Zebrafish Embryo on a Droplet ("Digital") Microfluidic Platform," Lab ...

https://en.wikipedia.org/wiki/Robin_L._Garrell

Microfluidic Analytical Techniques. 1. 2020. 760. 0.120. Why? Cell Line, Tumor. 2. 2022. 17106. 0.080. Why? ...

https://connects.catalyst.harvard.edu/Profiles/display/person/160504/network/researchareas/details

Therefore, new analytical techniques addressing single-cell behavior are the key for further optimization. In particular, state ... This systematic study revealed a maximum growth rate of μ_max=0.6 h^(-1) during microfluidic cultivation compared to μ_max=0.4 ... The aim of the present thesis was to develop and establish a new microfluidic platform technology for microbial single-cell ... In the present project, novel single-use microfluidic cultivation devices with structures in the sub-micrometer range for ...

https://www.avt.rwth-aachen.de/go/id/iavo/lidx/1/file/540646

This chapter reviews the up-to-date researches in the field of biosensors integrated with microfluidic techniques, most of ... Critical Reviews in Analytical Chemistry. 2016:213-223. *9. Mok J et al. Digital microfluidic assay for protein detection. ... This chapter reviews the up-to-date researches in the field of biosensors integrated with microfluidic techniques, most of ... Analytical Chemistry. 2013;. 85. (7):3532-3538. *27. Mooltongchun M, Teepoo S. A simple and cost-effective microfluidic paper- ...

https://www.intechopen.com/chapters/71835

An Analytical Solution to Neumann-Type Mixed Boundary Poiseuille Microfluidic Flow in Rectangular Channel Cross-Sections (Slip/ ... No-Slip) including a Numerical Technique to Derive It (Articles) Christiane Richter, Frederik Kotz, N. Keller, Tobias M. ...

https://scirp.org/journal/articles.aspx?searchcode=Christiane+Damg%C3%A9&searchfield=authors&page=1&skid=0

His group is focused on the study of interactions of proteins with nanostructured surfaces and their use in analytical ... Although a number of nanomaterials have been used in conjunction with traditional separation techniques, the small volumes and ... Additionally, he is developing microfluidic devices to monitor biologically active compounds. Carlos is engaged in various ... Combining these properties with their remarkable recognition capabilities has resulted in analytical systems with significantly ...

https://www.clemson.edu/health-research/faculty/garcia.html

Analytical costs for the uPAD technique were approximately 50 times lower than market-rate costs with ICPOES. Further, the uPAD ... The objective of this research was to evaluate a relatively new technology, microfluidic paper-based analytical devices (uPADs ... Fumes from three common welding techniques (shielded metal arc, metal inert gas, and tungsten inert gas welding) were sampled ... Rapid detection of transition metals in welding fumes using paper-based analytical devices. ...

http://www2a.cdc.gov/nioshtic-2/BuildQyr.asp?s1=chromium&f1=%2A&Adv=0&terms=1&PageNo=9&RecNo=85&View=f&

Using SERS-based microfluidic paper-based device (μPAD) for calibration-free quantitative measurement of AMI cardiac biomarkers ... Eryılmaz M, Yıldırım E, Tamer U. Lab on a chip: A versatile integration with spectroscopic techniques. Handbook on ... Miniaturization in Analytical Chemistry 2020. [DOI: 10.1016/b978-0-12-819763-9.00007-6] [Reference Citation Analysis] ...

https://wjgnet.com/1948-5182/CitedArticlesInF6?id=10.3390%2Fnano8090730

by a routine culture technique. The entire process was achieved in a single microfluidic chip within 35 min. In comparison to ... this new device performed well in regards to the analytical parameters of sensitivity, specificity and accuracy. Therefore, the ...

https://www.ikerlan.es/en/lines-of-research/publications/item/a-novel-real-time-micro-pcr-based-pointofcare-device-for-salmonella-detection-in-human-clinical-samples

YAMADA K, HENARES TG, SUZUKI K and CITTERIO D (2015) Paper‐based inkjet‐printed microfluidic analytical devices. Angew Chem. ... The accuracy of the proposed method in this paper was comparable with that of previously published techniques. It is worthwhile ... The microfluidic paper-based analytical device for the direct determination of ammonia in wastewater has been successfully ... JAYAWARDANE BM, MCKELVIE ID and KOLEV SD (2015) Development of a gas-diffusion microfluidic paper-based analytical device (μPAD ...

http://www.scielo.org.za/scielo.php?script=sci_arttext&pid=S1816-79502020000300015&lng=es&nrm=iso

Microchip Analytical Procedures E5.196.630 Microdissection E5.200.500.620.265 Microfluidic Analytical Techniques E5.196.630.465 ... Fluorescent Antibody Technique E5.478.412 Fluorescent Antibody Technique, Direct E5.478.412.300 Fluorescent Antibody Technique ... Post and Core Technique E6.323.428.100.500 E6.780.346.250.500 E6.323.528.250.500 E7.695.190.88.500 E6.780.345.250.500 E6.780. ... Two-Hybrid System Techniques E5.393.525.870 E5.601.870 Tylophora B1.650.940.800.575.100.99.875 B1.650.940.800.575.100.81.958 ...

https://decs.bvs.br/P/mnchg2016.txt

The technique has many potential applications, including miniaturizing standard analytical tests performed in chemical ... But current microfluidic devices have a key limitation: they can only effectively handle liquids with high surface tension, ...

https://www.utoronto.ca/news/nature-inspired-coatings-could-power-lab-chip-devices-rapid-inexpensive-medical-tests

microfluidic (4). *microfluidic analytical techniques (1). *midazolam (2). *omeprazole (3). *omeprazole sulfone (1) ... Evaluation of seven drug metabolisms and clearances by cryopreserved human primary hepatocytes cultivated in microfluidic ... Evaluation of seven drug metabolisms and clearances by cryopreserved human primary hepatocytes cultivated in microfluidic ... The microfluidic perfusion played a part in stabilizing and partially recovering the levels of the HNF4α, PXR, OAPT2, CYP 1A2, ...

https://accounts.public.ce.basespace.illumina.com/b/search/article.nb?id=22830982

To achieve this, two techniques were combined and used: T-Junction microfluidics and electrohydrodynamic (EHD) processing. The ... Microfluidic Analytical Techniques, Nanofibers, Organosilicon Compounds, Polyglycolic Acid, Polylactic Acid-Polyglycolic Acid ... of controlled porosity particle/fibre loaded scaffolds using a hybrid micro-fluidic and electrohydrodynamic technique. ... To achieve this, two techniques were combined and used: T-Junction microfluidics and electrohydrodynamic (EHD) processing. The ...

https://www.ndorms.ox.ac.uk/publications/491867

Microfluidic Analytical Technique. Technique, Microfluidic Analytical. Techniques, Microfluidic Analytical. Tree number(s):. ... Analytical Techniques, Microfluidic Microfluidic Analyses Microfluidic Analysis Microfluidic Analytical Technique Technique, ... Analyses, Microfluidic. Analysis, Microfluidic. Analytical Technique, Microfluidic. Analytical Techniques, Microfluidic. ... Microfluidic Analytical Techniques Entry term(s). Analyses, Microfluidic Analysis, Microfluidic Analytical Technique, ...

https://decs.bvsalud.org/en/ths/resource/?id=38816

To date, while there exist many techniques for inducing microalgal cells to produce and accumulate lipid with high efficiency, ... few analytical methods are available for characterizing a population of such lipid-accumulated microalgae including E. gracilis ... which consists of an optical time-stretch microscope and a fluorescence analyzer on top of an inertial-focusing microfluidic ... This method provides a promise for evaluating the efficiency of lipid-inducing techniques for biofuel production, which is also ...

https://spie.org/Publications/Proceedings/Paper/10.1117/12.2245836

TrAC Trends in Analytical Chemistry, 28(3), 336-346. doi:10.1016/j.trac.2008.11.014. Hamilton, D. J., Ambrus, Á., Dieterle, R. ... Picó, Y., Fernández, M., Ruiz, M. J., & Font, G. (2007). Current trends in solid-phase-based extraction techniques for the ... Yakovleva, J., Davidsson, R., Lobanova, A., Bengtsson, M., Eremin, S., Laurell, T., & Emnéus, J. (2002). Microfluidic Enzyme ... TrAC Trends in Analytical Chemistry, 28(4), 404-415. doi:10.1016/j.trac.2008.12.006. Zui, O. V., & Birks, J. W. (2000). Trace ...

https://riunet.upv.es/handle/10251/33727

Tailor-made microfluidic devices (analytical chemistry) are key in developing improved tritium-detection strategies ( ... These approaches are complemented by ultrasensitive characterization techniques using hyperfine spectroscopy (Pöppl), ... microfluidic devices (Belder) and experimental isotope separation and T-detection (Fischer).. Interdisciplinarity in 1,2,3H ... from the fundamental quantum mechanical principles to more applied aspects in the implementation phase involving analytical and ...

https://www.uni-leipzig.de/en/123h/profile

Microfluidic Analytical Techniques. (3 times). IMV (2 times). PDMS (2 times). AP (1 time). ≫ ... 2004 Cardiac chamber and coronary artery doses associated with postmastectomy radiotherapy techniques to the chest wall and ...

https://allie.dbcls.jp/short/exact/Any/LAD.html

Microfluidic Analytical Techniques. Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and ... Analytical, Diagnostic and Therapeutic Techniques and Equipment11. Cathode Ray TubeBioelectric Energy SourcesElectrodesStem ... Cell ResearchFlow CytometryThermodilutionImage CytometryCell SeparationMicrofluidic Analytical TechniquesBlood Flow Velocity ... DysfunctionFlow CytometryThermodilutionImage CytometryRegional Blood FlowCell SeparationMicrofluidic Analytical TechniquesBlood ...

https://lookformedical.com/en/search/cells

Microfluidic Analytical Techniques Microwaves Nitric Acid Oxidation-Reduction Paper Particulate Matter Polyethylenes Quaternary ... Title : A Microfluidic Paper-Based Analytical Device for Rapid Quantification of Particulate Chromium Personal Author(s) : ... A Microfluidic Paper-Based Analytical Device for Rapid Quantification of Particulate Chromium. ... A Microfluidic Paper-Based Analytical Device for Rapid Quantification of Particulate Chromium ...

https://stacks.cdc.gov/view/cdc/29789

... a reagent enclosed in the microfluidic channel; and a substance inlet to the microfluidic channel; a centrifugal device ... A substance determination system comprises a chip comprising: a substrate; a microfluidic channel disposed on the substrate; ... It is critical for microfluidic devices aiming to be used as an alternative to conventional analytical techniques to have ... Diagnostics for the developing world: microfluidic paper-based analytical devices. EP3134211B1 (en) 2021-09-15. Microfluidic ...

https://patents.google.com/patent/US20180313765A1/en

Microfluidic Analytical Techniques * Microscopy, Electron, Transmission * Microscopy, Fluorescence * Nanoparticles * ... "Microfluidic-SERS Devices for One Shot Limit-of-Detection." ,i>ANALYST,/i> 139 (13). ROYAL SOC CHEMISTRY: 3227-34.,detail:{ ... "Microfluidic-SERS Devices for One Shot Limit-of-Detection." ,i>ANALYST,/i> 139.13 (2014): 3227-3234. Print.,pubMedId: ... i>ANALYST,/i>, ,i>139,/i>(13), 3227-3234.,capCitation:,span class=\title\>\n ,span>Microfluidic-SERS devices for one shot ...

https://med.stanford.edu/profiles/nathan-burrows

Experience in analytical techniques (e.g. optical microscopy, flow cytometry, qPCR). *Experience in fabrication of microfluidic ... We envisage that the postdoc has some experience of neutron scattering techniques. The intended use of the magnetic contrast ... We are looking for postdoctoral fellow which will be driving the development of magnetic reference layers, using PVD techniques ... A strong background in sample growth and characterisation using scattering techniques is required. Good computer skills and ...

http://www.phd-positions.dk/?tag=uppsala

Synchronization techniques. Term project. ECE 414 Wireless Systems 4R-0L-4C W. Prerequisites: ECE 310 Corequisites: There are ... Design process, modeling; analytical and numerical. Actuators; dynamics and thermal issues. Use of software for layout and ... Microsensors, microfluidic systems, applications in engineering, biology, chemistry, and physics. Cross-listed with ME 519, EP ... Industrial testing techniques for AC and DC tests of mixed-signal integrated circuits using an automatic test environment (ATE ...

https://rose-hulman.edu/academics/course-catalog/2021-2022/programs/Computer%20Engineering/course-descriptions.html

A new proteomics technique for analyzing 3-nitrotyrosine-containing peptides is presented here. This technique is based on the ... Microfluidic interfaces coupled to ESI mass spectrometers hold great potential for proteomics as they have been shown to ... term somewhat more than a decade ago the field of proteomics witnessed a rapid growth mainly fueled by instrumental analytical ... Proteome identification using peptide-centric proteomics techniques is a routinely used analysis technique. One of the most ...

https://www.researchgate.net/profile/Kenny-Helsens

Microfluidic Devices - Key Technologies to Enable Real-Time Patient Monitoring and Treatment. Monday, 2 October 2017 at 15:00 ... The same measurement techniques are used in patients and in experimental models allowing genuine translational research. Martyn ... He develops novel analytical science methods using microfluidics, electrochemical sensors / biosensors, and wireless ... Microfluidic Devices - Key Technologies to Enable Real-Time Patient Monitoring and Treatment. Biosensors and Biosecurity Summit ...

http://selectbiosciences.com/conferences/biographies.aspx?conf=BIOSEC2017&speaker=412232

Suitable for technique. 3D imaging (42). Ablation (7). Airyscan (7). Confocal (31). FLIM (5). FRAP (20). FRET (22). High ...

https://miap.eu/journal_article/negative-pressure-induced-droplet-generation-in-a-microfluidic-flow-focusing-device-analytical-chemistry2017-2/

In Microfluidic Biosensors (pp. 159-170). Academic Press. (Read it) 2022 Baretta, R., Raucci, A., Cinti, S.,* & Frasconi, M. ( ... Advanced techniques for manufacturing paper-based microfluidic analytical devices. ... Advanced techniques for manufacturing paper-based microfluidic analytical devices. In Microfluidic Biosensors (pp. 159-170). ... In Handbook of Smart Materials in Analytical Chemistry (pp. 179-209). Chichester, UK: John Wiley & Sons, Ltd. (Read it) ...

https://uninanobiosensors.com/publications/

Milavanovic L, Ma H, Microfluidic technique for measuring friction between single cells and engineered surfaces, Analytical ... Santoso AT, Deng X, Lee JH, Matthews K, Duffy SP, Islamzada E, Myrand-Lapierre M, McFaul SM, Ma H, Microfluidic Cell-phoresis ... Todenhöfer T, Park S, Jin C, Ang RR, Duffy SP, Matthews K, Abdi H, Ma H, Black PC, Microfluidic enrichment of circulating tumor ... Matthews K, Duffy SP, Myrand-Lapierre M, Ang RR, Li L, Scott MD, Ma H, Microfluidic analysis of red blood cell deformability as ...

https://cbr.ubc.ca/our-people/investigators/hongshen-ma/

Electrophoretic separations comprise a group of analytical tech- niques such as capillary zone electrophoresis, isoelectric focusing, isota- chophoresis and free ﬂow electrophoresis. (amcaonline.org.ar)

His group is focused on the study of interactions of proteins with nanostructured surfaces and their use in analytical chemistry. (clemson.edu)
Combining these properties with their remarkable recognition capabilities has resulted in analytical systems with significantly improved performance and novel applications across physics, chemistry, biology, and engineering. (clemson.edu)
Interdisciplinarity in 1,2,3 H adds competence compared to traditional programmes in chemistry or physics, e.g. by providing a sufficiently broad basis for understanding the multi-faceted properties of hydrogen isotopes, from the fundamental quantum mechanical principles to more applied aspects in the implementation phase involving analytical and synthetic chemistry as well as chemical technology. (uni-leipzig.de)
Analytical Chemistry , DOI:10.1021/acs.analchem.1c03856. (uninanobiosensors.com)
Analytical Chemistry , 93 (41), 14007-14013. (uninanobiosensors.com)
TrAC Trends in Analytical Chemistry , 116374. (uninanobiosensors.com)
He has a PhD in Analytical Chemistry, Mass Spectrometry. (redshiftbio.com)
Rob completed formal training in Analytical Chemistry at RMIT University in 2004 with Professor Philip Marriott. (edu.au)
Robert Shellie has a PhD in Analytical Chemistry from RMIT University. (edu.au)

Additionally, he is developing microfluidic devices to monitor biologically active compounds. (clemson.edu)

These approaches are complemented by ultrasensitive characterization techniques using hyperfine spectroscopy (Pöppl), microfluidic devices (Belder) and experimental isotope separation and T-detection (Fischer). (uni-leipzig.de)
Burlington, MA - RedShift BioAnalytics, Inc. (RedShiftBio), a provider of innovative analytical solutions for protein characterization used in biotherapeutic drug development, appoints Nigel Danielson-Ewing as Director of Product Management. (redshiftbio.com)
The appointment is a key part of the company's strategy for accelerated growth following the successful launch of the AQS³pro, a novel platform filling a critical gap in the protein characterization analytical toolkit. (redshiftbio.com)

Each device contained up to several thousand micrometer sized cultivation structures in parallel intended for high-throughput analysis of single cells and isogenic microcoloniesIn the present research two major single-cell investigations were performed demonstrating the universal applicability and potential of the microfluidic single-cell cultivation technology:(i) Growth analysis of industrially relevant bacteria (in particular E. coli and C. glutamicum) with single-cell resolution was performed. (rwth-aachen.de)
In comparison to the culture reference method that is used in the clinical laboratories, this new device performed well in regards to the analytical parameters of sensitivity, specificity and accuracy. (ikerlan.es)
The purpose of this study was to develop and evaluate the effectiveness of the microfluidic paper-based device (µPAD) for the quantification of ammonia in wastewater. (scielo.org.za)
2012). It has been suggested that a low-cost microfluidic paper-based analytical device offers an opportunity to tackle this need, thereby increasing the frequency and geographic coverage of environmental monitoring while also reducing analytical costs and complexity of measurement (Meredith et al. (scielo.org.za)
Here we demonstrate a high-throughput optofluidic Euglena gracilis profiler which consists of an optical time-stretch microscope and a fluorescence analyzer on top of an inertial-focusing microfluidic device that can detect fluorescence from lipid droplets in their cell body and provide images of E. gracilis cells simultaneously at a high throughput of 10,000 cells/s. (spie.org)
Our team at the Group of Microfluidics and Complex Fluids will use these data to design and fabricate an innovative microfluidic device utilizing SRS microscopy for label-free, rapid leukaemia cell detection, which will help in quick and reliable differentiation between different types of leukemia cells providing clinicians with quantitative information that can be applied to diagnostics. (edu.pl)
Multi-dimensional gas chromatography with a planar microfluidic device for the characterisation of volatile oxygenated organic compounds. (edu.au)
Cheng-Hsing Lin, Cheng-Kuan Su, Yuh-Chang Sun, Development of Online Microdialysis Microfluidic-Based Photocatalyst-Assisted Vaporization Device Inductively Coupled Plasma-Mass Spectrometry Hyphenated Analytical System for in vivo Quantification of the Transition of Brain Extracellular Mercury after Thimerosal Administration, Microchem. (speciation.net)

This chapter reviews the up-to-date researches in the field of biosensors integrated with microfluidic techniques, most of which are publications within the last 5 years. (intechopen.com)
He develops novel analytical science methods using microfluidics, electrochemical sensors / biosensors, and wireless electronics to make portable, wearable monitoring devices. (selectbiosciences.com)
In Microfluidic Biosensors (pp. 159-170). (uninanobiosensors.com)
Colorimetric, electrochemical, or fluorescence techniques might be the transduction mechanisms for paper-based biosensors for SARS-CoV-2 RNA detection. (fcdijack.com)
This includes: spectroscopy-based techniques, lab-on-a-chip, mass spectrometry (including MALDI-TOF and proteomics), and the development of ultra-small biosensors. (pittcon.org)

To achieve this, two techniques were combined and used: T-Junction microfluidics and electrohydrodynamic (EHD) processing. (ox.ac.uk)
Paper microfluidics is a transformational technology that enables the development of inexpensive analytical devices based on designs printed in wax-based ink on chromatography paper. (ojp.gov)

Rapid detection of transition metals in welding fumes using paper-based analytical devices. (cdc.gov)
The objective of this research was to evaluate a relatively new technology, microfluidic paper-based analytical devices (uPADs), for measuring the metals content in welding fumes. (cdc.gov)
Although a number of nanomaterials have been used in conjunction with traditional separation techniques, the small volumes and low analysis time offered by microfluidic devices represent an open field for innovation with unique opportunities in terms of research and training. (clemson.edu)
But current microfluidic devices have a key limitation: they can only effectively handle liquids with high surface tension, such as water. (utoronto.ca)
In this presentation I will describe the development of 3D printed microfluidic devices connected to wireless electronics for transplant organ and patient monitoring. (selectbiosciences.com)
Advanced techniques for manufacturing paper-based microfluidic analytical devices. (uninanobiosensors.com)
Fingerprinting techniques operating over the network are proposed to identify various aspects of industrial control systems (ICSs) including software, hardware, and physical devices. (gatech.edu)
This fact sheet reports on the key benefits and underlying National Institute of Justice (NIJ) research on paper microfluidic devices, which provide police and forensic evidence-collection teams with an easily stored and reliable tool for presumptive testing of unknown materials. (ojp.gov)
With NIJ support, Dr. Bruce McCord at Florida International University has developed a suite of paper microfluidic devices for forensic residue analysis applications, such as presumptive testing of explosives, serological stains, and detection of seized drugs. (ojp.gov)
Microscale analysis methods, such as microfluidic devices and capillary electrophoresis, can treat a pL~nL order solution rapidly and sensitively, so that they are expected to be basis of single-cell-level trace bioanalysis. (elsevier.com)

Our proprietary Microfluidic Modulation Spectroscopy technology addresses a key unmet need in the rapidly growing protein therapeutics development market. (redshiftbio.com)
The company has developed a powerful new analytical technique, Microfluidic Modulation Spectroscopy (MMS) that enables direct probing of the biophysical structure of proteins. (redshiftbio.com)

In contrast to conventional systems, for example, fluorescence activated cell sorting, microfluidic cultivations enable the analysis of cell dynamics by automated time-lapse microscopy with full spatio-temporal resolution. (rwth-aachen.de)

Develop a microfluidic cell sorter for isolation of selected leukemia cells based on their Raman signal, optimize the system for increased sensitivity and selectivity and automatize the setup for high throughput. (edu.pl)
UC San Francisco scientists have used a high-throughput CRISPR-based technique to rapidly map the functions of nearly 500 genes in human cells, many of them never before studied in detail. (phys.org)

Microfluidic-Based Bacteria Isolation from Whole Blood for Diagnostics of Blood Stream Infection. (cdc.gov)

The modified version of the colorimetric method using Nessler reagent was combined with microfluidic technologies to create a low-cost monitoring system for detection of ammonia in wastewater. (scielo.org.za)
Relevant analytical data such as limits of detection, dynamic ranges, recoveries, and sample pretreatments are recorded in three tables. (upv.es)
Targeted proteomics via selected reaction monitoring is a powerful mass spectrometric technique affording higher dynamic range, increased specificity and lower limits of detection than other shotgun mass spectrometry methods when applied to proteome analyses. (researchgate.net)
In order to cope with the extremely low available sample volume, the low Hg concentration and the high salinity of the sample matrix, a microfluidic approach was developed based on microdialysis for sampling and photocatalyst-assisted vaporization for sample introduction to the ICP-MS. Under optimal operation conditions, the developed analytical system provided limits of detection (based on 3s) of 2.7 and 1.7 ng/L for MMHg and TMHg. (speciation.net)

Further single-cell analysis exposed that solely the medium composition was the growth enhancing factor, rather than the continuous perfusion during single-cell cultivation or the analytical method itself. (rwth-aachen.de)

Cooperate with the younger members of the team providing them support in the development and integration of the other microfluidic modules (for manipulation of single cells preferentially by optical tweezers, surface acoustic waves or hydrodynamic traps). (edu.pl)

Therefore, new analytical techniques addressing single-cell behavior are the key for further optimization. (rwth-aachen.de)
The successful candidate will join a multinational and interdisciplinary team of scientists and will be responsible for i) designing, constructing and testing microfluidic cell sorters separating leukemia cellsfrom WBCs, ii) integration ofthe cell sorter with the Stimulated Raman Spectrometer (SRS) and iii) optimization and automation ofthe systemfor increased sensitivity, selectivity and throughput. (edu.pl)

Single cell analysis of microbial production strains in microfluidic biore. (rwth-aachen.de)
The aim of the present thesis was to develop and establish a new microfluidic platform technology for microbial single-cell analysis in order to address key concerns on population heterogeneity and reactor inhomogeneity in industrial biotechnology. (rwth-aachen.de)
Therefore, various lab-scale cultivations were performed and results compared with our microfluidic single-cell analysis. (rwth-aachen.de)
Proteome identification using peptide-centric proteomics techniques is a routinely used analysis technique. (researchgate.net)
His research primarily focuses on food, flavour, and fragrance analysis using hyphenated chromatographic techniques. (edu.au)
Rob joined the Centre for Food Innovation in August 2022 where his research and research training focuses on adopting new techniques and developing cutting edge separation science approaches for chemical analysis of food and flavour. (edu.au)
JH Analytical provides insight into using vacuum coating in sample preparation and EDS analysis. (jh-analytical.com)

However, these analytical methods each have their own restrictions, and different levels of investment in equipment, operating costs, secondary pollution, and other deficiencies (Guo et al. (scielo.org.za)
To date, while there exist many techniques for inducing microalgal cells to produce and accumulate lipid with high efficiency, few analytical methods are available for characterizing a population of such lipid-accumulated microalgae including E. gracilis with high throughout, high accuracy, and single-cell resolution simultaneously. (spie.org)
however, practical single-cell-level analytical methods are quite limited. (elsevier.com)

In particular, state-of-the-art microfluidic cultivation systems facilitating single-cell resolution and accurate environmental control over long time periods at the same time, offer completely new experimental assays on microbial populations. (rwth-aachen.de)
The same measurement techniques are used in patients and in experimental models allowing genuine translational research. (selectbiosciences.com)

The development and use of techniques to study physical phenomena and construct structures in the nanoscale size range or smaller. (lookformedical.com)
RedShiftBioⓇ is a provider of innovative analytical instrumentation for the research, development and manufacture of protein therapeutic drugs. (redshiftbio.com)

Point-of-care (POC) diagnostics is a discipline focused on using instrumentation or analytical techniques to detect and identify target analytes at or near a patient in need of treatment, whereby the patient is properly diagnosed and provided the necessary medical care. (datacite.org)

Technology is powering developments in biomedicine, with advances in computing power and imaging techniques providing just some of the solutions to today's biggest health issues. (pittcon.org)
This article will cover recent advances in clinical and biomedical science and the ways in which these techniques could be applied to tackle current global health threats. (pittcon.org)

A Review of Analytical Techniques and Their Application in Disease Diagnosis in Breathomics and Salivaomics Research. (cdc.gov)

Microfluidic Technologies for Deformability based Cell Sorting. (ubc.ca)
In September 2015, he embraced an opportunity for industry-academia mobility as an investigator in the ARC Training Centre for Portable Analytical Separation Technologies (ASTech). (edu.au)

Microfluidic paper-based analytical instruments are promising candidates because they are low-cost, easy-to-use, quick, accurate, and long-lasting in a wide range of settings. (fcdijack.com)

These techniques have been miniaturized in the last years and now represent one of the most im- portant applications of the lab-on-a-chip technology. (amcaonline.org.ar)

A technique encompassing morphometry, densitometry, neural networks, and expert systems that has numerous clinical and research applications and is particularly useful in anatomic pathology for the study of malignant lesions. (lookformedical.com)

The RApID (Raman Imaging for Diagnostics) project aims to develop the first Stimulated Raman Scattering (SRS) microscopic-microfluidic system for non-invasive imaging of live cells and apply it to rapid leukaemia cell imaging, diagnostics and sorting. (edu.pl)
March 17 marks the start of Pittcon 2019, which will highlight a range of biomedical techniques that can provide improved diagnostics, better treatments and faster discovery. (pittcon.org)

The technique has many potential applications, including miniaturizing standard analytical tests performed in chemical laboratories. (utoronto.ca)

Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. (lookformedical.com)
It is a useful technique for live blood cell imaging. (bioimager.com)

High time resolutions can be achieved using a novel droplet based microfluidic system. (selectbiosciences.com)
We developed and evaluated NSAG techniques for Group III-Nitrides as a way to mitigate the various difficulties with this material system (high defect density, threading dislocations, phase separation and graining, etc. (gatech.edu)

Kang N, Guo Q , Islamzada E , Ma H , Scott MD, Microfluidic determination of lymphocyte vascular deformability: effects of intracellular complexity and early immune activation, Integrative Biology, 10(4) 207-217, 2018. (ubc.ca)

At Pittcon 2019, Ester Segal will provide an overview of PRISM, a chip-based spectroscopic technique that can correctly guide a physician to the best antibiotic for an infection, reducing the misuse of these vital drugs. (pittcon.org)

This method provides a promise for evaluating the efficiency of lipid-inducing techniques for biofuel production, which is also applicable for identifying biomedical samples such as blood cells and cancer cells. (spie.org)

Platelet-targeting sensor reveals thrombin gradients within blood clots forming in microfluidic assays and in mouse. (jefferson.edu)
In this paper, the microfluidic size-separation technique pinched flow fractionation (PFF) is used to separate cancer cells from white blood cells (WBCs). (ox.ac.uk)

Compared to a typical 1 liter lab-scale batch cultivation, interestingly a 1.5-fold enhanced growth rate of C. glutamicum wild type cells under constant microfluidic cultivation conditions was found. (rwth-aachen.de)

Analytical strategy photodegradation/chemiluminescence/continuous-flow multicommutation methodology for the determination of the herbicide Propanil. (upv.es)

Anatomy1

Chemicals and Drugs11

Analytical, Diagnostic and Therapeutic Techniques and Equipment46

Phenomena and Processes9

Disciplines and Occupations7

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture12

Information Science3

Health Care14

Geographicals1

Metabolite fingerprinting: detecting biological features by independent component analysis. (1/1368)

Microviscoelasticity of the apical cell surface of human umbilical vein endothelial cells (HUVEC) within confluent monolayers. (2/1368)

Formation of droplets of alternating composition in microfluidic channels and applications to indexing of concentrations in droplet-based assays. (3/1368)

Computerized microfluidic cell culture using elastomeric channels and Braille displays. (4/1368)

Syntheses of 11C- and 18F-labeled carboxylic esters within a hydrodynamically-driven micro-reactor. (5/1368)

Wetting morphologies at microstructured surfaces. (6/1368)

Controlling nonspecific protein adsorption in a plug-based microfluidic system by controlling interfacial chemistry using fluorous-phase surfactants. (7/1368)

High-throughput mouse genotyping using robotics automation. (8/1368)

No images available that match "microfluidic analytical techniques"

Microfluidic Analytical Techniques

Microfluidics

Dimethylpolysiloxanes

Canada

Chemistry Techniques, Analytical

Lab-On-A-Chip Devices

Mass Spectrometry

Electrophoresis, Capillary

Biosensing Techniques

Equipment Design

Microchip Analytical Procedures

Electrophoresis, Microchip

Metabolomics

Spectrometry, X-Ray Emission

Antibodies, Immobilized

Microtechnology

Chromatography, High Pressure Liquid

Reproducibility of Results

Miniaturization

Gas Chromatography-Mass Spectrometry

Food Analysis

Printing

Environmental Monitoring

Calibration

Chromatography, Gas

Single-Cell Analysis

Magnetic Resonance Spectroscopy

Equipment Failure Analysis

Hydrodynamics

Flow Injection Analysis

Spectrometry, Mass, Electrospray Ionization

Health Surveys

Sensitivity and Specificity

Silicones

Spectrophotometry, Atomic

Electrochemical Techniques

Time Factors

Pharmaceutical Preparations

Polymethyl Methacrylate

Cell Culture Techniques

Chromatography, Liquid

Nanotechnology

Spectroscopy, Fourier Transform Infrared

Cycloparaffins

Data Interpretation, Statistical

Longitudinal Studies

Electroosmosis

Models, Statistical

Models, Biological

Microchemistry

Molecular Structure

Logistic Models

Proteins

Polycarboxylate Cement

Glass

Reference Standards

Cluster Analysis

Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Algorithms

Paper

Research Design

Electrowetting

Surface Properties

Silicon

Capillary Action

Oxidation-Reduction

Point-of-Care Systems

Health Services Research

Age Distribution

Analytic Sample Preparation Methods

Cells

Immunoassay

Biological Markers

Polystyrenes

Electrodes

Limit of Detection

Cross-Sectional Studies

Metabolite fingerprinting: detecting biological features by independent component analysis. (1/1368)

Microviscoelasticity of the apical cell surface of human umbilical vein endothelial cells (HUVEC) within confluent monolayers. (2/1368)

Formation of droplets of alternating composition in microfluidic channels and applications to indexing of concentrations in droplet-based assays. (3/1368)

$Patent US7094345 - Implementation of microfluidic components, including molecular fractionation ... - Google Patents$ Patent US7094345 - Implementation of microfluidic components, including molecular fractionation ... - Google Patents

$Patent US7094345 - Implementation of microfluidic components, including molecular fractionation ... - Google Patents$ Patent US7094345 - Implementation of microfluidic components, including molecular fractionation ... - Google Patents