Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.
Group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. It is inherited as an X-linked or autosomal recessive defect. Mutations occurring in many different genes cause human Severe Combined Immunodeficiency (SCID).
A strain of non-obese diabetic mice developed in Japan that has been widely studied as a model for T-cell-dependent autoimmune insulin-dependent diabetes mellitus in which insulitis is a major histopathologic feature, and in which genetic susceptibility is strongly MHC-linked.
Transplantation between animals of different species.
Syndromes in which there is a deficiency or defect in the mechanisms of immunity, either cellular or humoral.
Experimental transplantation of neoplasms in laboratory animals for research purposes.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
An encapsulated lymphatic organ through which venous blood filters.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
A serine-threonine protein kinase that, when activated by DNA, phosphorylates several DNA-binding protein substrates including the TUMOR SUPPRESSOR PROTEIN P53 and a variety of TRANSCRIPTION FACTORS.
An interleukin receptor subunit that was originally discovered as a component of the INTERLEUKIN 2 RECEPTOR. It was subsequently found to be a component of several other receptors including the INTERLEUKIN 4 RECEPTOR, the INTERLEUKIN 7 RECEPTOR, the INTERLEUKIN-9 RECEPTOR, the INTERLEUKIN-15 RECEPTOR, and the INTERLEUKIN-21 RECEPTOR. Mutations in the gene for the interleukin receptor common gamma chain have been associated with X-LINKED COMBINED IMMUNODEFICIENCY DISEASES.
The transfer of lymphocytes from a donor to a recipient or reinfusion to the donor.
Progenitor cells from which all blood cells derive.
Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.
Blood of the fetus. Exchange of nutrients and waste between the fetal and maternal blood occurs via the PLACENTA. The cord blood is blood contained in the umbilical vessels (UMBILICAL CORD) at the time of delivery.
Transference of cells within an individual, between individuals of the same species, or between individuals of different species.
Ordered rearrangement of B-lymphocyte variable gene regions coding for the IMMUNOGLOBULIN CHAINS, thereby contributing to antibody diversity. It occurs during the differentiation of the IMMATURE B-LYMPHOCYTES.
Transfer of HEMATOPOIETIC STEM CELLS from BONE MARROW or BLOOD between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). Hematopoietic stem cell transplantation has been used as an alternative to BONE MARROW TRANSPLANTATION in the treatment of a variety of neoplasms.
A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.
In vivo methods of screening investigative anticancer drugs, biologic response modifiers or radiotherapies. Human tumor tissue or cells are transplanted into mice or rats followed by tumor treatment regimens. A variety of outcomes are monitored to assess antitumor effectiveness.
The survival of a graft in a host, the factors responsible for the survival and the changes occurring within the graft during growth in the host.
Recombinases involved in the rearrangement of immunity-related GENES such as IMMUNOGLOBULIN GENES and T-CELL RECEPTOR GENES.
Form of passive immunization where previously sensitized immunologic agents (cells or serum) are transferred to non-immune recipients. When transfer of cells is used as a therapy for the treatment of neoplasms, it is called adoptive immunotherapy (IMMUNOTHERAPY, ADOPTIVE).
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Animals or humans raised in the absence of a particular disease-causing virus or other microorganism. Less frequently plants are cultivated pathogen-free.
Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.
Mice bearing mutant genes which are phenotypically expressed in the animals.
The ability of lymphoid cells to mount a humoral or cellular immune response when challenged by antigen.
An individual that contains cell populations derived from different zygotes.
High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.
Antibodies produced by a single clone of cells.
Transplantation of STEM CELLS collected from the fetal blood remaining in the UMBILICAL CORD and the PLACENTA after delivery. Included are the HEMATOPOIETIC STEM CELLS.
A cell line derived from cultured tumor cells.
Semisynthetic conjugates of various toxic molecules, including RADIOACTIVE ISOTOPES and bacterial or plant toxins, with specific immune substances such as IMMUNOGLOBULINS; MONOCLONAL ANTIBODIES; and ANTIGENS. The antitumor or antiviral immune substance carries the toxin to the tumor or infected cell where the toxin exerts its poisonous effect.
Transference of fetal tissue between individuals of the same species or between individuals of different species.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.
An organism that, as a result of transplantation of donor tissue or cells, consists of two or more cell lines descended from at least two zygotes. This state may result in the induction of donor-specific TRANSPLANTATION TOLERANCE.
Differentiation antigens expressed on B-lymphocytes and B-cell precursors. They are involved in regulation of B-cell proliferation.
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.
Ordered rearrangement of T-cell variable gene regions coding for the antigen receptors.
Form of adoptive transfer where cells with antitumor activity are transferred to the tumor-bearing host in order to mediate tumor regression. The lymphoid cells commonly used are lymphokine-activated killer (LAK) cells and tumor-infiltrating lymphocytes (TIL). This is usually considered a form of passive immunotherapy. (From DeVita, et al., Cancer, 1993, pp.305-7, 314)
Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
The transfer of leukocytes from a donor to a recipient or reinfusion to the donor.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
In vivo method of screening investigative anticancer drugs and biologic response modifiers for individual cancer patients. Fresh tumor tissue is implanted under the kidney capsule of immunocompetent mice or rats; gross and histological assessments follow several days after tumor treatment in situ.
Forceful administration into the peritoneal cavity of liquid medication, nutrient, or other fluid through a hollow needle piercing the abdominal wall.
The transference of BONE MARROW from one human or animal to another for a variety of purposes including HEMATOPOIETIC STEM CELL TRANSPLANTATION or MESENCHYMAL STEM CELL TRANSPLANTATION.

Prevention of collagen-induced arthritis by gene delivery of soluble p75 tumour necrosis factor receptor. (1/9154)

Collagen type II-induced arthritis (CIA) in DBA/1 mice can be passively transferred to SCID mice with spleen B- and T-lymphocytes. In the present study, we show that infection ex vivo of splenocytes from arthritic DBA/1 mice with a retroviral vector, containing cDNA for the soluble form of human p75 receptor of tumour necrosis factor (TNF-R) before transfer, prevents the development of arthritis, bone erosion and joint inflammation in the SCID recipients. Assessment of IgG subclass levels and studies of synovial histology suggest that down-regulating the effector functions of T helper-type 1 (Th1) cells may, at least in part, explain the inhibition of arthritis in the SCID recipients. In contrast, the transfer of splenocytes infected with mouse TNF-alpha gene construct resulted in exacerbated arthritis and enhancement of IgG2a antibody levels. Intriguingly, infection of splenocytes from arthritic DBA/1 mice with a construct for mouse IL-10 had no modulating effect on the transfer of arthritis. The data suggest that manipulation of the immune system with cytokines, or cytokine inhibitors using gene transfer protocols can be an effective approach to ameliorate arthritis.  (+info)

Probing the function of Bordetella bronchiseptica adenylate cyclase toxin by manipulating host immunity. (2/9154)

We have examined the role of adenylate cyclase-hemolysin (CyaA) by constructing an in-frame deletion in the Bordetella bronchiseptica cyaA structural gene and comparing wild-type and cyaA deletion strains in natural host infection models. Both the wild-type strain RB50 and its adenylate cyclase toxin deletion (DeltacyaA) derivative efficiently establish persistent infections in rabbits, rats, and mice following low-dose inoculation. In contrast, an inoculation protocol that seeds the lower respiratory tract revealed significant differences in bacterial numbers and in polymorphonuclear neutrophil recruitment in the lungs from days 5 to 12 postinoculation. We next explored the effects of disarming specific aspects of the immune system on the relative phenotypes of wild-type and DeltacyaA bacteria. SCID, SCID-beige, or RAG-1(-/-) mice succumbed to lethal systemic infection following high- or low-dose intranasal inoculation with the wild-type strain but not the DeltacyaA mutant. Mice rendered neutropenic by treatment with cyclophosphamide or by knockout mutation in the granulocyte colony-stimulating factor locus were highly susceptible to lethal infection by either wild-type or DeltacyaA strains. These results reveal the significant role played by neutrophils early in B. bronchiseptica infection and by acquired immunity at later time points and suggest that phagocytic cells are a primary in vivo target of the Bordetella adenylate cyclase toxin.  (+info)

Vascular endothelial growth factor (VEGF)-mediated angiogenesis is associated with enhanced endothelial cell survival and induction of Bcl-2 expression. (3/9154)

Vascular endothelial growth factor (VEGF) is an endothelial cell mitogen and permeability factor that is potently angiogenic in vivo. We report here studies that suggest that VEGF potentiates angiogenesis in vivo and prolongs the survival of human dermal microvascular endothelial cells (HDMECs) in vitro by inducing expression of the anti-apoptotic protein Bcl-2. Growth-factor-enriched and serum-deficient cultures of HDMECs grown on collagen type I gels with VEGF exhibited a 4-fold and a 1.6-fold reduction, respectively, in the proportion of apoptotic cells. Enhanced HDMEC survival was associated with a dose-dependent increase in Bcl-2 expression and a decrease in the expression of the processed forms of the cysteine protease caspase-3. Cultures of HDMECs transduced with and overexpressing Bcl-2 and deprived of growth factors showed enhanced protection from apoptosis and exhibited a twofold increase in cell number and a fourfold increase in the number of capillary-like sprouts. HDMECs overexpressing Bcl-2 when incorporated into polylactic acid sponges and implanted into SCID mice exhibited a sustained fivefold increase in the number of microvessels and a fourfold decrease in the number of apoptotic cells when examined 7 and 14 days later. These results suggest that the angiogenic activity attributed to VEGF may be due in part to its ability to enhance endothelial cell survival by inducing expression of Bcl-2.  (+info)

Suppression of angiogenesis, tumorigenicity, and metastasis by human prostate cancer cells engineered to produce interferon-beta. (4/9154)

We determined whether the IFN-beta gene can be used to suppress angiogenesis, tumor growth, and metastasis of human prostate cancer cells growing in the prostate of nude mice. Highly metastatic PC-3M human prostate cancer cells were engineered to constitutively produce murine IFN-beta subsequent to infection with a retroviral vector containing murine IFN-beta cDNA. Parental (PC-3M-P), control vector-transduced (PC-3M-Neo), and IFN-beta-transduced (PC-3M-IFN-beta) cells were injected into the prostate (orthotopic) or subcutis (ectopic) of nude mice. PC-3M-P and PC-3M-Neo cells produced rapidly growing tumors and regional lymph node metastases, whereas PC-3M-IFN-beta cells did not. PC-3M-IFN-beta cells also suppressed the tumorigenicity of bystander nontransduced prostate cancer cells. PC-3M-IFN-beta cells produced small tumors (3-5 mm in diameter) in nude mice treated with anti-asialo GM1 antibodies and in severe combined immunodeficient/Beige mice. Immunohistochemical staining revealed that PC-3M-IFN-beta tumors were homogeneously infiltrated by macrophages, whereas control tumors contained fewer macrophages at their periphery. Most tumor cells in the control tumors were stained positive by an antibody to proliferative cell nuclear antigen; very few were positively stained by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling. In sharp contrast, PC-3M-IFN-beta tumors contained fewer proliferative cell nuclear antigen-positive cells and many terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling-positive cells. Staining with antibody against CD31 showed that control tumors contained more blood vessels than PC-3M-IFN-beta tumors. PC-3M-IFN-beta cells were more sensitive to lysis mediated by natural killer cells in vitro or to cytostasis mediated by macrophages than control transduced cells. Conditioned medium from PC-3M-IFN-beta cells augmented splenic cell-mediated cytolysis to control tumor cells, which could be neutralized by antibody against IFN-beta. Collectively, the data suggest that the suppression of tumorigenicity and metastasis of PC-3M-IFN-beta cells is due to inhibition of angiogenesis and activation of host effector cells.  (+info)

Induction of lasting complete regression of preformed distinct solid tumors by targeting the tumor vasculature using two new anti-endoglin monoclonal antibodies. (5/9154)

Endoglin (EDG, CD105) is a proliferation-associated antigen on endothelial cells. In this study, two new anti-EDG monoclonal antibodies (mAbs) Y4-2F1 (or termed SN6j) and P3-2G8 (SN6k) were generated and used for treating distinct preformed tumors. These mAbs, both IgG1-kappa antibodies, cross-reacted weakly with mouse endothelial cells but defined epitopes different from the epitope defined by a previously reported anti-EDG mAb K4-2C10 (B. K. Seon et al., Clin. Cancer Res., 3: 1031-1044, 1997). SN6j and SN6k reacted strongly with human endothelial cells and vascular endothelium of malignant human tissues but showed no significant reactivity with tumor cells per se. The deglycosylated ricin A chain (dgRA) conjugates of the two mAbs showed a weak but specific cytotoxic activity against murine endothelial cells in vitro. In the therapeutic studies, severe combined immunodeficient mice were inoculated s.c. with MCF-7 human breast cancer cells and left untreated until palpable tumors of distinct size (4-6 mm in diameter) appeared. Mice with the distinct tumors were treated by i.v. administration of individual anti-EDG conjugates, unconjugated mAbs, or a control conjugate. Long-lasting complete regression of the tumors was induced in the majority of tumor-bearing mice (n = 8 for each conjugate) when 40 microg of the individual conjugates were administered three times via the tail vein. It is remarkable that the tumors remained regressed without further therapy for as long as the mice were followed (i.e., 100 days). Control conjugate did not induce regression of the tumors in any of the treated mice, although weak nonspecific effects were observed in some of the mice (n = 8). The effects of unconjugated mAbs were small with the dose used, i.e., 34 microg three times. The anti-EDG conjugates showed antiangiogenic activity in the dorsal air sac assay in mice. The results suggest good potential of these conjugates for the clinical application.  (+info)

Protective effect of vitamin E on ischaemia-reperfusion injury in ovarian grafts. (6/9154)

Ovarian cortical tissue cryopreservation with subsequent autografting is a potential strategy for the preservation of fertility in patients undergoing systemic chemotherapy and pelvic radiotherapy. Non-vascular implants are first subjected to a period of ischaemia before revascularization and are, therefore, vulnerable to ischaemia-reperfusion injury from reactive oxygen species. Ischaemia-reperfusion injury was investigated during the first week after surgery in murine ovarian grafts and human ovarian xenografts in mice with severe combined immune deficiency (SCID) by measuring total lipid peroxides and malondialdehyde concentrations with a colorometric assay. The effects of administering an antioxidant, vitamin E, on these concentrations were also tested. Products of lipid peroxidation were higher in non-supplemented murine autografts compared with control ovaries (P < 0.05), and were significantly reduced on day 3 by vitamin E administration (P < 0.05). Similarly, in human xenografts, there was a significant reduction in lipid peroxidation with vitamin E administration. These results correspond to a significantly greater total follicle survival in the murine grafts of the supplemented group (45 versus 72%; P < 0.05). They suggest that antioxidant treatment improves the survival of follicles in ovarian grafts by reducing ischaemia-reperfusion injury.  (+info)

Two neutralizing human anti-RSV antibodies: cloning, expression, and characterization. (7/9154)

BACKGROUND: Respiratory syncytial virus (RSV) infection is a major problem in the newborn and aging populations. Fully human monoclonal antibodies with the ability to neutralize RSV could have a major impact on the immunotherapy of the disease. The generation of human antibodies has been difficult because there exists no general way to activate B cells against an antigen of choice in vitro. MATERIALS AND METHODS: Human spleen cells from individuals exposed to RSV were used to repopulate SCID mice. Hu-SCID mice were boosted with RSV fusion (F)-protein and subsequently developed B cell tumors. The tumors were removed and cultured and subcloned in vitro, using a feeder layer of CD154-expressing T cells. Two of these tumors produced the antibodies designated RF-1 and RF-2. VL genes were isolated by standard PCR techniques, however, it was necessary to use high-temperature reverse transcriptase to clone the VH genes. RESULTS: RF-1 and RF-2 VH genes were both found to be closely related members of the VH2 family. Vk genes originated from the VK III family. RF-1 and RF-2 recombinant antibodies expressed in CHO cells (cRF-1 and cRF-2) were found to have affinities for RSV F-protein of 0.1 nM and 0.07 nM, respectively, and both were able to neutralize several A and B subtypes of RSV. CONCLUSION: The technique of immortalizing human B lymphocytes, by passage in SCID mice and expression as recombinant antibodies in CHO cells, provides a method by which high-affinity human antibodies can be developed for immunotherapy of viral diseases.  (+info)

Tolerance to antigen-presenting cell-depleted islet allografts is CD4 T cell dependent. (8/9154)

Pretreatment of pancreatic islets in 95% oxygen culture depletes graft-associated APCs and leads to indefinite allograft acceptance in immunocompetent recipients. As such, the APC-depleted allograft represents a model of peripheral alloantigen presentation in the absence of donor-derived costimulation. Over time, a state of donor-specific tolerance develops in which recipients are resistant to donor APC-induced graft rejection. Thus, persistence of the graft is sufficient to induce tolerance independent of other immune interventions. Donor-specific tolerance could be adoptively transferred to immune-deficient SCID recipient mice transplanted with fresh immunogenic islet allografts, indicating that the original recipient was not simply "ignorant" of donor antigens. Interestingly, despite the fact that the original islet allograft presented only MHC class I alloantigens, CD8+ T cells obtained from tolerant animals readily collaborated with naive CD4+ T cells to reject donor-type islet grafts. Conversely, tolerant CD4+ T cells failed to collaborate effectively with naive CD8+ T cells for the rejection of donor-type grafts. In conclusion, the MHC class I+, II- islet allograft paradoxically leads to a change in the donor-reactive CD4 T cell subset and not in the CD8 subset. We hypothesize that the tolerant state is not due to direct class I alloantigen presentation to CD8 T cells but, rather, occurs via the indirect pathway of donor Ag presentation to CD4 T cells in the context of host MHC class II molecules.  (+info)

Fingerprint Dive into the research topics of Lectin histochemistry of human leukaemic mast cells (HMC-1) transplanted into severe combined immunodeficient (scid) mice. Together they form a unique fingerprint. ...
Recent developments of surrogate assays for human hematopoietic stem cells (HSC) have facilitated efforts at improving HSC gene transfer efficiency. Through the use of xenograft transplantation models, such as nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mice, successful oncoretroviral gene transfer to transplantable hematopoietic cells has been achieved. However, because of the low frequency and/or homing efficiency of SCID repopulating cells (SRC) in bone marrow (BM), studies have primarily focused on cord blood (CB). The recently developed extended (| 60 days) long-term culture-initiating cell (ELTC-IC) assay detects an infrequent and highly quiescent candidate stem cell population in BM as well as CB of the CD34(+)CD38(-) phenotype. Although these characteristics suggest that ELTC-IC and SRC might be closely related, attempts to oncoretrovirally transduce ELTC-IC have been unsuccessful. Here, recently developed conditions (high concentrations of SCF + FL + Tpo in serum-free medium)
The SCID mouse model for rheumatoid arthritis (RA) is an established and reliable approach to examining the distinct mechanisms operative in RA synovium, and evaluating novel gene therapy strategies. However, serum concentrations of circulating gene therapy products following gene transfer are frequently too low to allow detection. This problem stimulated us to develop a novel implantation technique to improve the yield of these soluble gene products. Synovial fibroblasts from patients with RA were cultured, passaged, and transduced with Ad5 sTNFRp55:Ig. sTNFRp55:Ig production was confirmed by ELISA, and then cells were implanted into SCID mice using a novel implantation strategy in which pieces of human cartilage were engrafted into a fibroblast-saturated inert sponge. Thereafter, the sponges were implanted under the skin of the mice instead of under the kidney capsule, as in the original approach, allowing co-implantation of larger pieces of cartilage together with higher numbers of ...
The generation of the immune system is the only known developmental process in mammals that utilizes site-specific genomic recombination mechanisms. B lymphocyte differentiation occurs in fetal liver...
Shao et al presented their findings at the 100th AACR meeting in Denver, Co. The team identified an angiogenic role for YKL-40 in tumor growth and development. Ectopic expression of YKL-40 in MDA-MB-231 breast cancer cells and HCT-116 colon cancer cells led to an extensive angiogenic phenotype of xenografted tumors in SCID/Beige mice and these tumors were 4-8 fold larger than ...
CD3+ T cells in severe combined immunodeficiency (scid) mice. II. Transplantation of dm2 lymphoid cells into semi-allogeneic scid mice | Angelika Rudolphi; Sibylle Spiess; Peter Conradt; Mogens H. Claesson; Jörg Reimann | download | BookSC. Download books for free. Find books
Hill, Laurie Lynn, Growth and metastasis of human melanoma in SCID mice: the effect of human natural killer cells on tumor growth in vivo (1993). Theses. 379 ...
Swine with severe combined immunodeficiency (SCID) are an emerging large animal model for biomedical research. There have been several SCID pig models described since our first discovery of naturally occurring SCID with mutations in Artemis (DCLRE1C) in 2012. SCID animals are particularly useful in biomedical research due to their lack of T, B, and sometimes NK cells. Absence of the adaptive immune system allows for human cell and tissue xenotransplantation into these SCID animals. The works described within this thesis are categorized under four main goals: (1) further characterization of the immune system of Art-/- SCID pigs, (2) development of a method for in utero injection of human stem cells, (3) in utero injection of human stem cells for the study of human immune cell engraftment within Art-/- IL2RG-/Y SCID pigs, and (4) development of an ovarian carcinoma model in SCID pigs. Characterization of the SCID pig immune system is important so that researchers can understand how components of the
To investigate infectious agents prospectively, suitable in vivo laboratory models are needed. This poses a problem in research involving such human-specific pathogens as HIV. To this end, xenochimeric models have been developed by transplanting immunodeficient mice with cellular targets of HIV, i.e., either human PBL (8, 9) or pieces of human fetal tissues such as liver and thymus containing hematopoietic cells (SCID-hu) (10, 11); although suitable to study some aspects of HIV in vivo, both models are limited by systematic issues (1). Human PBL transplanted into SCID mice (hu-PBL-SCID) are activated within the xenogeneic environment, T cells become successively anergic, and, because of lack of both continuing hematopoiesis and appropriate hu-PBL maintenance, the xenograft is nonfunctional within several weeks (1, 9). In contrast, mice transplanted with human fetal liver and thymus (SCID-hu) de novo generate and maintain human cells, especially T cells, within the human thymus graft (10). ...
If a child is diagnosed and treated within the first few months of life before the child has a serious infection, then the long-term survival rate is more than 90%. With early treatment, most children with SCID should be able to develop their own working immune system. The best course of treatment for a child with SCID depends on several factors including the type of SCID, the childs health, and doctor recommendations.. Most infants with SCID are treated with HSCT, or bone marrow transplant, which results in a new immune system that is able to fight infection. In HSCT, doctors take healthy blood-forming cells that can develop into a healthy immune system from a donor and put them into a child. The donor cells provide the child with an immune system.. Another less common but promising treatment option is gene therapy, which is currently in clinical trials. In gene therapy, doctors extract a childs defective blood-forming cells, correct the defect, and put the corrected cells back into the ...
The study is the first combined analysis of more than 3 million infants screened for SCID in 10 states and the Navajo Nation. Infants from participating programs born from the start of the first pilot program in January 2008 through July 2013 were included.. In May, 2010, SCID was the 29th condition added to the national recommended uniform panel for newborn screened disorders, and California began screening newborns statewide on August 15, 2010, just four years ago. Currently, 23 states conduct newborn screening for SCID, and the test is performed for nearly two-thirds of infants born across the country. Now that infants with SCID are being detected at a very young age, we can tailor protection and early treatment for them while they are still healthy, without having to also treat the complications from infections that result from the disease. This leads to the best outcomes in terms of survival and immune reconstitution, said senior author Jennifer Puck, MD, a pediatric immunologist at UCSF ...
On February 26, 2009 Barb was at the meeting today where the advisory committee voted on whether or not to add SCID to the panel for newborn screening. Unfortunately, they voted NO. However, Barb did say that we shouldnt give up hope. For now they voted against recommending SCID to the Secretary for newborn screening. BUT, they want to see if certain criteria can be met and then they will revote. A group of committee members are supposed to work out the criteria tonight and tell the committee in the morning exactly what they want to see. One thing they want to see is if either the Wisconsin or Mass pilot programs find a SCID baby. Neither one has, although theyve found 2 DiGeorge babies that wouldnt have been picked up yet and Wisconsin found a baby with a neutrophil defect that is so rare that its only the 2nd case diagnosed. They reported that the baby has now had a successful transplant. That child wouldnt have lived if it were not for the Wisconsin pilot study.. Im so sorry that I ...
|p||strong|Estimated Removal of Live Colony date: 16 July 2020.|/strong||/p|NSG-nude (or NSG-Foxn1|sup|null|/sup|) mice are NOD.|em|scid|/em|.|em|Il2Rγc|sup|null|/sup||/em| (NSG) animals with the |em|Foxn1|sup|null|/sup||/em| knockout allele. The NSG model is permissive for human xenografts, with Foxn1-deficiency (lack of thymus) allowing human stem cell-derived, thymic epithelial cell (TEC) precursors to reconstitute the only thymopoeitic effector population. NSG-nude mice are useful for studying the function of human TECs in disease models of type 1 diabetes, autoimmunity and transplantation.
These mice are doubly homozygous for the severe combined immunodeficiency (scid) mutation and the beta2m null allele. They lack MHC class I, exhibit low NK cell activity, and support markedly elevated levels of human T cell engraftment.
嚴重聯合免疫缺陷病(severe combined immunodeficiency disease,SCID)、Swiss型無丙球蛋白血症、胸腺淋巴組織發育不良和網狀組織發育不全是一種重型免疫缺陷病。其特點是先天性和遺傳性B細胞性T細胞系統異常。本組疾病呈常染色體隱性或-連鎖遺傳。50%SCID有陽性家族史。伴髮網狀組織發育不全的SCID系由原始造血幹細胞缺陷引起;Swiss型無丙球蛋白血症是淋巴幹細胞缺陷引起;部分SCID則由T細胞分化不良與B細胞成熟障礙所致。
Principal Investigator:TAGUCHI Osamu, Project Period (FY):1997 - 1999, Research Category:Grant-in-Aid for Scientific Research (C), Section:一般, Research Field:Experimental pathology
Abstract: Over the last years novel genetic defects causing SCID have been discovered, and the molecular and immunological mechanisms of SCID have been better characterized. Distinct forms of SCID show both common and peculiar (e.g., absence or presence of nonimmunological features) aspects, and they are currently classified into six groups according to prevalent pathophysiological mechanisms: impaired cytokine-mediated signaling; pre-T cell receptor defects; increased lymphocyte apoptosis; defects in thymus embryogenesis; impaired calcium flux; other mechanisms.This review is the updated, extended and largely modified translation of the article Cossu F: Le basi genetiche delle SCID, originally published in Italian language in the journal Prospettive in Pediatria 2009, 156:228-238.The initial clinical manifestations of SCID (Severe Combined Immunodeficiency), a heterogeneous group of genetic defects with an overall incidence of about 1 in 40,000 to 75,000 newborns [1-3], are most frequently ...
A mother of a baby with severe combined immunodeficiency (SCID) responds to the upcoming film Everything, Everything, which takes on bubble baby disease.
California officially begins a Development Program for SCID Newborn Screening today, August 16, 2010! The program will screen all infants born in the state, roughly 520,000 babies per year, for low or absent T lymphocytes. It will continue until approximately 1 million babies have been tested. Any positive results that are found will be followed by a. ...
Much has changed in the field of immunology since David Vetter, the young Texas boy affectionately known as the boy in the bubble, was born in 1971. David had one of the most documented and studied cases of SCID in history, capturing the worlds attention for 12 years as he lived inside a protective plastic. ...
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DNA-dependent protein kinase (DNA-PK), consisting of a Ku heterodimer (Ku70/80) and a large catalytic subunit (DNA-PKcs), plays an important role in the repair of DNA double-strand breaks via non-homologous end-joining (NHEJ) in mammalian cells. Severe combined immunodeficient (scid) mice carry a mutation in the gene encoding DNA-PKcs and are sensitive to ionizing radiation. To examine the roles of DNA-PKcs in the generation of deletion mutations in vivo, we crossed scid mice with gpt delta transgenic mice for detecting mutations. The scid and wild-type (WT) gpt delta transgenic mice were irradiated with a single X-ray dose of 10 Gy, and Spi− mutant frequencies (MFs) were determined in the brain and spleen 2 days after irradiation. Irradiation with X-rays significantly enhanced Spi− MF in both organs in the scid and WT mice. The MFs in the brain of irradiated scid mice were significantly lower than those in WT mice, i.e., 2.9 ± 1.0 × 10− 6 versus 5.0 ± 1.1 × 10− 6 (P | 0.001), respectively.
TY - JOUR. T1 - Development of functional human blood and immune systems in NOD/SCID/IL2 receptor γ chainnull mice. AU - Ishikawa, Fumihiko. AU - Yasukawa, Masaki. AU - Lyons, Bonnie. AU - Yoshida, Shuro. AU - Miyamoto, Toshihiro. AU - Yoshimoto, Goichi. AU - Watanabe, Takeshi. AU - Akashi, Koichi. AU - Shultz, Leonard D.. AU - Harada, Mine. PY - 2005/9/1. Y1 - 2005/9/1. N2 - Here we report that a new nonobese diabetic/severe combined immunodeficient (NOD/SCID) mouse line harboring a complete null mutation of the common cytokine receptor γ chain (NOD/SCID/interleukin 2 receptor [IL2r] γnull) efficiently supports development of functional human hemato-lymphopoiesis. Purified human (h) CD34+ or hCD34 +hCD38- cord blood (CB) cells were transplanted into NOD/SCID/IL2rγnull newborns via a facial vein. In all recipients injected with 105 hCD34+ or 2 × 10 4 hCD34+hCD38- CB cells, human hematopoietic cells were reconstituted at approximately 70% of chimerisms. A high percentage of the human ...
TY - JOUR. T1 - Determination of engraftment potential of human cord blood stem-progenitor cells as a function of donor cell dosage and gestational age in the NOD/SCID mouse model. AU - Ural, Serdar. AU - Sammel, Mary D.. AU - Blakemore, Karin J.. PY - 2005/9/1. Y1 - 2005/9/1. N2 - Objective: The purpose of this study was to determine cell dosage parameters for successful engraftment of human cord blood hematopoietic stem cells (HSC) using an in vivo assay system, and to determine if there are differences with donor gestational age. Study design: HSCs were transplanted into nonobese diabetic-severe combined immunodeficient (NOD/SCID) mice. Donor cell dosage and gestational age ranges were 1 to 40×106 CD34+ cells per mouse, and 23 to 40 weeks, respectively. Recipient bone marrow was assessed for engraftment capacity of the HSCs. Results: There was increasing engraftment levels with increasing dosages of transplanted HSCs. When controlled for donor HSC dosage, engraftment levels using donor cord ...
Severe combined immunodeficiency (Scid) mice have defects in V(D)J recombination and DNA double-strand breaks repair caused by an inherited genetic defect in the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs). Scid mice are highly susceptible to development of T-cell lymphomas, and because of the nature of its association with DNA repair and recombination, DNA-PKcs is considered to belong to the caretaker class of tumor suppressor genes. In the present study, the susceptibility of Scid mice to colon carcinogenesis due to administration of azoxymethane (AOM) was investigated. Significantly higher susceptibility in terms of induction of both aberrant crypt foci (ACFs), putative pre-cancerous lesions of the colon and colon cancers was observed as compared with the isogenic strain, C.B-17 mice. The incidences of colon tumors, either adenomas or adenocarcinomas, in Scid and C.B-17 mice after administration of AOM (10 mg/kg body weight/week) for 6 weeks were 87% (26 of 30) and 50% (15 of ...
TY - JOUR. T1 - Noninvasive MR imaging of magnetically labeled stem cells to directly identify neovasculature in a glioma model. AU - Anderson, Stasia A.. AU - Glod, John. AU - Arbab, Ali S.. AU - Noel, Martha. AU - Ashari, Parwana. AU - Fine, Howard A.. AU - Frank, Joseph A.. PY - 2005/1/1. Y1 - 2005/1/1. N2 - Bone marrow-derived endothelial precursor cells incorporate into neovasculature and have been successfully used as vehicles for gene delivery to brain tumors. To determine whether systemically administered Sca1+ bone marrow cells labeled with superparamagnetic Iron oxide nanoparticles can be detected by in vivo magnetic resonance imaging in a mouse brain tumor model, mouse Sca1+ cells were labeled in vitro with ferumoxides-poly-L- lysine complexes. Labeled or control cells were administered intravenously to glioma-bearing severe combined immunodeficient (SCID) mice. Magnetic resonance imaging (MRI) was performed during tumor growth. Mice that received labeled cells demonstrated ...
Abstract: Objective. To explore the therapeutic potential of CD147/HAb18 mAb in the treatment of RA in severe combined immunodeficiency (SCID) mice engrafted with human cartilage and rheumatoid synovium tissue (SCID-HuRAg).. Methods. SCID-HuRAg mice were treated separately with CD147/HAb18 mAb, anti-TNF- mAb or a combination of both. The mice in control group were treated with anti-Japanese encephalitis virus mAb. The volume of engrafts was measured and the number of inflammatory cells and cartilage erosion score were examined. Expression of MMP-2, -3 and -9 was determined by immunohistochemistry. Human inflammatory cytokine levels in mouse sera were assessed using cytometric bead array kit.. Results. The volume of engrafts decreased significantly in SCID-HuRAg mice treated separately with anti-CD147 mAb or anti-TNF- mAb, and in the mice treated with anti-CD147 mAb plus anti-TNF- mAb (P , 0.05). Significant reduction was observed in cartilage erosion score in anti-CD147 treatment group and ...
Mice with the autosomal recessive severe combined immune deficiency (scid) mutation lack mature lymphocytes because of defective joining of T cell receptor and immunoglobulin (Ig) gene segments. Penetrance of this mutation is incomplete since 10-25% of SCID mice produce some T or B lymphocytes. This leaky phenotype could be due to a reversion of the mutation in some mice or to a constant, low frequency of functional lymphocytes generated in all SCID mice with variable survival of such cells. We report here that all SCID mice can be stimulated to produce functional B cells by the transfer of normal neonatal, but not adult, T cells. T cell-induced rescue of C.B-17scid B cells results in high levels of Ig expressing the Ighb allotype of the SCID recipient. These results show that all SCID mice generate some functional B cells, the majority of which do not survive in the absence of a subset of T cells present in high frequency in the neonate ...
As for lung cancer, investigators have chosen a number to utilize xenograft models of CaP. Unfortunately, CaP xenografts are far more fastidious than lung cancer xenografts, and the generation of models that are representative of typical human disease has only recently been accomplished. Until recently, the majority of research conducted for CaP relied on the cell lines PC-3, DU145, and LNCaP. Among these, only LNCaP cells exhibit androgen responsiveness and express the prostate-specific antigen (PSA) and androgen receptor (AR). Thus, the relevance of DU-145 and PC-3 cells to clinical CaP has been questioned. To overcome the shortage of representative models of human CaP, a number of investigators began establishing xenografts in immune-deficient scid/scid mice using samples obtained directly from patients [145-149]. These xenografts offered the following advantages: (1) the expansion of small amounts of starting clinical material, (2) the enrichment of relatively homogeneous cell populations ...
In contrast with the rapid progress in the characterization of murine CD34− HSCs, the analysis of human HSCs has proceeded more slowly. In 1997, Goodell et al. reported on human and monkey SP cells that showed a rapid Hoechst dye efflux activity. These SP cells were lineage-marker negative, revealed a highly enriched long-term culture-initiating cell (LTC-IC) capacity, and developed into CD34+ cells after stroma-supported cell culture [21], thereby suggesting that these HSCs were CD34−.. In a NOD/SCID mouse model, Bhatia et al. first described a novel HSC population from human cord blood (CB) samples with a Lin−CD34−CD38− phenotype [28]. These cells had low clonogenic activity in vitro, but remarkably, regenerated multilineage hematopoiesis in NOD/SCID mice. Of interest was that these CD34− HSCs could differentiate into CD34+ cells, resulting in a greater repopulating activity in cytokine-supported short-term cultures (2-4 days), whereas CD34+ HSCs lost their stem cell potential in ...
The SCID-hu mouse, engrafted with human hematolymphoid organs, is permissive for infection with the human immunodeficiency virus (HIV). This mouse model was used to test compounds for antiviral efficacy. Two weeks after infection with HIV, 100 percent (40/40) of SCID-hu mice were positive for HIV by the polymerase chain reaction. When first treated with 3-azido-3-deoxythymidine (AZT), none (0/17) were HIV-positive by this assay. However, AZT-treated SCID-hu mice did have a few infected cells; after AZT treatment was stopped, viral spread was detected by polymerase chain reaction in such mice. Thus, the SCID-hu mouse provides a means to directly compare new antiviral compounds with AZT and to further improve antiviral efficacy. ...
The relative efficiency of human HSC engraftment was the most important component of our comparison. Human HSCs are assayed by the functional SRC assay in which limiting numbers of cells are transplanted into NOD-scid mice and assayed for multilineage differentiation. Numerous studies from our group and others have enriched for SRCs based on sorting for CD34 and CD38 expression such that approximately 1 in 121 CD34+CD38−Lin− CB cells are SRCs in NOD/Lt-scid mice. In this study, we used a less enriched population, Lin− CB, and found approximately 1 in 6500 cells are SRCs in NOD/Lt-scid mice and 1 in 1800 cells in NSG mice. Thus, NSG mice were 3.6-fold more sensitive in detecting SRCs. Based on limiting dilution analysis in NSG mice, we found an approximate 9-fold improvement in SRC frequency in female mice compared with NOD/Lt-scid mice. Death of most male NOD/Lt-scid mice precluded accurate determination of SRC frequency (data not shown). The discovery of sex differences when limiting ...
The need for developing improved tumor xenograft models for evaluating anti-cancer therapies is essential as has previously been documented (27). The development of new genetically modified immunodeficient mouse models has greatly facilitated the successful engraftment and prolonged survival of normal and neoplastic human tissues (14). These newer generations of humanized mice bearing human tissue xenografts have begun to provide valuable insights into complex human biological systems including hematopoiesis, innate and adaptive immunity, autoimmunity, infectious diseases, regenerative medicine, and cancer (14). Our goal has been to utilize severely immunodeficient mice to establish xenografts of human tumors that include both the tumor cells and the associated tumor stroma, i.e., fibroblasts and inflammatory leukocytes. The implantation of human lung (7) and ovarian tumors (17) into NSG mice resulted in the long-term engraftment of the tumor and the tumor-associated nonmalignant cells that ...
TY - JOUR. T1 - Influence of age, irradiation and humanization on NSG mouse phenotypes. AU - Knibbe-Hollinger, Jaclyn S.. AU - Fields, Natasha R.. AU - Chaudoin, Tammy R.. AU - Epstein, Adrian A.. AU - Makarov, Edward. AU - Akhter, Sidra P.. AU - Gorantla, Santhi. AU - Bonasera, Stephen J.. AU - Gendelman, Howard E.. AU - Poluektova, Larisa Y.. PY - 2015/1/1. Y1 - 2015/1/1. N2 - Humanized mice are frequently utilized in bench to bedside therapeutic tests to combat human infectious, cancerous and degenerative diseases. For the fields of hematology-oncology, regenerative medicine, and infectious diseases, the immune deficient mice have been used commonly in basic research efforts. Obstacles in true translational efforts abound, as the relationship between mouse and human cells in disease pathogenesis and therapeutic studies requires lengthy investigations. The interplay between human immunity and mouse biology proves ever more complicated when aging, irradiation, and human immune reconstitution ...
A fundamental question in cancer biology is whether cells with tumorigenic potential are common or rare within human cancers. Studies on diverse cancers, including melanoma, have indicated that only rare human cancer cells (0.1-0.0001%) form tumours when transplanted into non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice. However, the extent to which NOD/SCID mice underestimate the frequency of tumorigenic human cancer cells has been uncertain. Here we show that modified xenotransplantation assay conditions, including the use of more highly immunocompromised NOD/SCID interleukin-2 receptor gamma chain null (Il2rg -/-) mice, can increase the detection of tumorigenic melanoma cells by several orders of magnitude. In limiting dilution assays, approximately 25% of unselected melanoma cells from 12 different patients, including cells from primary and metastatic melanomas obtained directly from patients, formed tumours under these more permissive conditions. In single-cell ...
The outbreak of the Zika virus (ZIKV) has been associated with increased incidence of congenital malformations. Although recent efforts have focused on vaccine development, treatments for infected individuals are needed urgently. Sofosbuvir (SOF), an FDA-approved nucleotide analog inhibitor of the Hepatitis C (HCV) RNA-dependent RNA polymerase (RdRp) was recently shown to be protective against ZIKV both in vitro and in vivo. Here, we show that SOF protected human neural progenitor cells (NPC) and 3D neurospheres from ZIKV infection-mediated cell death and importantly restored the antiviral immune response in NPCs. In vivo, SOF treatment post-infection (p.i.) decreased viral burden in an immunodeficient mouse model. Finally, we show for the first time that acute SOF treatment of pregnant dams p.i. was well-tolerated and prevented vertical transmission of the virus to the fetus. Taken together, our data confirmed SOF-mediated sparing of human neural cell types from ZIKV-mediated cell death in ...
What is a nude mouse and why is it used in cancer research? How come my mouse pups have a different coat color than their parents? What is a knockout mouse? Answers to these and more are in this introduction to the laboratory mouse, one of the most widely used models in biomedical research. We will explore the natural history and origin of the laboratory mouse; the ethics and regulations on the use of mice in research; the characteristics and nomenclature of commonly used mouse strains; the anatomy, physiology, and husbandry of mice; common mouse diseases and their effects on research; mouse coat color genetics and its relevance to human diseases; immunodeficient mouse models and their uses in research; and the technology for genetically engineering mice (e.g., transgenic mice). Video demonstrations of necropsy, mouse handling, anesthesia and surgery, identification methods, and research techniques will be provided. Each student is expected to read research papers that use the mouse as a ...
Although many biological mechanisms are similar in rodents and humans, there are several structural and functional differences that render the extrapolation of experimental results to clinical practice quite difficult. Multiple transgenic, knockout, and reconstituted models of autoimmune diseases have been developed over the past 2 decades. The creation of humanized mice, defined as immunodeficient mice engrafted with human hematopoietic stem cells or PBMCs, provided a powerful tool for preclinical testing of new immunomodulatory agents and study of human immune responses (25,28-36). This is particularly true in the case of ILT3, which, like other members of the Ig gene superfamily, has no ortholog in rodents.. In addition to T- and B-cell deficiency, NOD/SCID mice have functional defects of macrophages and natural killer cells (31-34) and high rates of human lymphocyte engraftment (34), providing a tool for studying human islet allograft rejection and the effect of immunomodulatory agents ...
Results presented in this study show that treatment with the combination of gemcitabine/doxorubicin improves the regulation of growth of HNSCC cells both in situ and in animal models, suggesting that this combination might offer an alternative treatment strategy against these cancers. Importantly, treatment of SCID mice bearing UM-SCC-22A xenografts with gemcitabine/doxorubicin combination significantly inhibited HNSCC tumor growth, which was concomitant with a significant increase in C18-ceramide levels. In addition, further analysis of a possible role for C18-ceramide, a product of LASS1 activity, in drug-induced cell death in situ, showed that treatment with gemcitabine/doxorubicin resulted in a significant increase in LASS1 expression and endogenous enzymatic activity of LASS1 for the generation of C18-ceramide in these cells. Mechanistically, the data obtained using molecular approaches either to partially inhibit or induce LASS1 expression in these cells revealed an important role of ...
The first SCID/BLAJ mouse model of dysferlinopathy was created by Dr. Ivan Torrente by backcrossing dysferlin deficient BLA/J mice onto the SCID strain while selecting for the retrotransposon insertion in intron 4 of the dysferlin gene, as well as the absence of specific B- and T- lymphocyte markers (CD4, CD8, and CD19) in cells isolated from peripheral blood. The result is a dysferlin deficient animal model that is also deficient in B and T lymphocytes, and thus more amenable to transplantation studies. Dr. Torrente made the mouse available to the reserach community in a private colony through the Jain Foundation, but due to low usage, the colony was cryopreserved.. The Jackson Laboratories created a second strain of SCID/BLAJ mice in their own laboratories in 2012, which are currently available in live repository.. Mutation ...
This is a monumental triumph to children born with severe combined immunodeficiency (SCID) and their families. Newborn screening will detect this problem early before complications develop, and allow for prompt life-saving interventions such as bone marrow transplantation. Usually the diagnosis of SCID is either missed or delayed until the age of 6-12 months of age, after patients suffer from severe and recurrent infections, and sometimes irreversible damage to organs such as the lungs. Newborn screening for SCID will no doubt save many lives and prevent suffering of children.. Professor Chaim Roifman, MD, ...
Mesenchymal stem cells (MSCs) have been shown to migrate to various tissues. There is little information on the fate and potential therapeutic efficacy of the reinfusion of MSCs following total body irradiation (TBI). We addressed this question using human MSC (hMSCs) infused to nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice submitted to TBI. Further, we tested the impact of additional local irradiation (ALI) superimposed to TBI, as a model of accidental irradiation. NOD/SCID mice were transplanted with hMSCs. Group 1 was not irradiated before receiving hMSC infusion. Group 2 received only TBI at a dose of 3.5 Gy, group 3 received local irradiation to the abdomen at a dose of 4.5 Gy in addition to TBI, and group 4 received local irradiation to the leg at 26.5 Gy in addition to TBI. Fifteen days after gamma irradiation, quantitative and spatial distribution of the hMSCs were studied. Histological analysis of mouse tissues confirmed the presence of radio-induced lesions in the ...
The imperative for better, more clinically predictive models of human cancer is obvious. Tumor graft models (also known as patient-derived xenografts, or PDX) are based on the transfer of primary tumors directly from the patient into an immunodeficient mouse. To accomplish this end, patient tumors must be obtained fresh from surgery, at which point they are mechanically or chemically digested, with a small portion saved as a primary stock, and established in a nonobese diabetic/severe combined immunodeficient (NOD/SCID) mouse. This breed of mouse lacks natural killer cells and is considered more immunodeficient than a nude mouse. PDX models are maintained by passaging cells directly from mouse to mouse once the tumor burden becomes too high. Tumors can be engrafted heterotopically or orthotopically. Heterotopic PDX models involve implanting tumors into the subcutaneous flank of a mouse. This method allows for easier cell transfer and precise monitoring of tumor growth and location (4). ...
Gene therapy is a way to change the genes a person has. The therapy is done by inserting a normal gene that a person is missing, or replacing a gene that is defective in some way.. An example of this type of therapy is for severe combined immunodeficiency disease (SCID). This is a very rare, life-threatening disease that a child may be born with. SCID causes a child to have very little or no immune system. This means he or she cant fight normal infections. SCID is also known as the boy in the bubble syndrome. Living in a normal environment can cause death to a child with a SCID. About 1 in 50,000 to 100,000 children is born with this disease.. Gene therapy for SCID is done by taking the childs blood and putting the normal gene into the blood cells. The child is then given a blood transfusion with his or her own blood that has the normal gene inserted. The gene then works itself into the immune system and lessens the symptoms of the disorder. Gene therapy is currently only available in ...
Summary: This zebrafish xenotransplant model of glioblastoma enables in vivo imaging of tumor cells and rapid screening for anti-glioma agents. It provides standardization of a model that is easily replicated across laboratories. ...
WALTHAM, Mass. - December 17, 2014 - PerkinElmer, Inc., a global leader focused on improving the health and safety of people and the environment, today announced the first commercially available screening test in the United States and Canada for Severe Combined Immunodeficiency (SCID), also know...
SEATTLE -- Before Gov. Chris Gregoire left office, she included money for newborn screening in her final budget. The screening looks for a rare condition called SCID -- Severe Combined Immunodeficiency.
So Katlyn got to play with her little friend Cole. Cole was diagnosed with SCID as well and they were both in the IWK at the same time. During which time we became very close with his whole family. We were very lucky they opened their home up to us and allowed us to stay the night in order to be as close to the hospital as possible for early morning. Thank you guys so much. The kids had a great time. I know Katlyn will be very excited when we are going back and she sees Cole again. It was very nice to see Katlyn interact with a child her age, something she has never been able to do before ...
Cancer stem cells (CSCs) have been defined as a unique subpopulation in tumors that possess the ability to initiate tumor growth and sustain tumor ...
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నెల్లూరు: మనుబోలు మండలం బద్వేలు క్రాస్‌రోడ్డు దగ్గర కారు బోల్తా, ముగ్గురికి గాయాలు,కర్నూలు: 16 వ రోజు జగన్ ప్రజా సంకల్ప యాత్ర,రంగారెడ్డి: మైలార్‌దేవ్‌పల్లిలో కింగ్స్‌ కాలనీలో ముస్తఫా అనే వ్యక్తిపై దుండగుల కాల్పులు,కడప: జగన్ సీఎం అయితే తన ఆస్తులు పెరుగుతాయి..చంద్రబాబు సీఎంగా ఉంటే ప్రజల ఆస్తులు పెరుగుతాయి: మంత్రి సోమిరెడ్డి,సిరిసిల్ల: అన్ని గ్రామాల్లో కేసీఆర్ గ్రామీణ ప్రగతి ...
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UCSF scientists have engineered human immune cells that can precisely locate diseased cells anywhere in the body and execute a wide range of customizable responses, including the delivery of drugs or other therapeutic payloads directly to tumors or other unhealthy tissues.
Moreover, SCID mice and Mongolian gerbil can be experimentally infected. The life cycle is basically similar to those of other ...
"bcl-2 antisense therapy chemosensitizes human melanoma in SCID mice". Janssen. February 1, 1998. Retrieved 24 November 2019. " ... "Gallium disrupts bacterial iron metabolism and has therapeutic effects in mice and humans with lung infections". Retrieved 5 ...
Genetically modified mice are the most common genetically engineered animal model. They have been used to study and model ... Fischer A, Hacein-Bey-Abina S, Cavazzana-Calvo M (June 2010). "20 years of gene therapy for SCID". Nature Immunology. 11 (6): ... "Knockout Mice". Nation Human Genome Research Institute. 2009. "GM pigs best bet for organ transplant". Medical News Today. 21 ... Rudolf Jaenisch created the first GM animal when he inserted foreign DNA into a mouse in 1974. The first company to focus on ...
SCID chimeric mice. In 28th Cologne workshop on Dope Analysis (Manfred Donike workshop) (Vol. 18, pp. 52-61). Sportverlag ...
Smith, C. I., Hassan, M. S. (1991). "Biological half-life of normal and truncated human IgG3 in scid mice". Eur J Immunol. 21 ( ...
"Helicobacter bilis-induced inflammatory bowel disease in scid mice with defined flora". Infection and Immunity. 65 (11): 4858- ... 2000]. H. rappini has also been isolated from the feces of healthy people, dogs, and mice, as well as from patients with ... Like Helicobacter hepaticus, it colonizes the bile, liver, and intestine of mice, and is associated with multifocal chronic ... nov., a novel Helicobacter species isolated from bile, livers, and intestines of aged, inbred mice". Journal of Clinical ...
"High level engraftment of NOD/SCID mice by primitive normal and leukemic hematopoietic cells from patients with chronic myeloid ... "A cell initiating human acute myeloid leukaemia after transplantation into SCID mice". Nature. 367 (6464): 645-648. Bibcode: ... Mouse models of breast cancer metastasis Marusyk, A; Polyak, K (2010). "Tumor heterogeneity: Causes and consequences". ...
Dick developed an in vivo repopulation assay using the NOD/SCID mouse. This technique of using an immune-deficient mouse to ... "A cell initiating human acute myeloid leukaemia after transplantation into SCID mice". Nature. 367 (6464): 645-8. doi:10.1038/ ... "Identification of primitive human hematopoietic cells capable of repopulating NOD/SCID mouse bone marrow: Implications for gene ... Dick is also known for his demonstration of a blood stem cell's ability to replenish the blood system of a mouse, his ...
Valproic scid. Valproic acid in mice studies restored histone acetylation levels, increased levels of pro-survival factors, and ... are first tested in mouse models of SMA created through a variety of mutations in the mouse SMN1 gene. If the mice show ... Tested on: mouse (M), only mouse cells (MC), human (H), Drosophila (D), rat (R). Successful treatment: yes (y), yes but with ... Sodium butyrate was the first HDAC inhibitor tested in SMA mouse models. It prolonged SMA mouse life span by 35% and showed ...
A cell initiating human acute myeloid leukaemia after transplantation into SCID mice. „Nature". 367 (6464), s. 645-8, Feb 1994 ... Homeobox gene expression in the intestinal epithelium of adult mice. „J Biol Chem". 266 (5), s. 3246-51, Feb 1991. PMID: ... An Mll-AF9 fusion gene made by homologous recombination causes acute leukemia in chimeric mice: a method to create fusion ... OTT-MAL fusion oncogene activates RBPJ-mediated transcription and induces acute megakaryoblastic leukemia in a knockin mouse ...
"DNA repair kinetics in SCID mice Sertoli cells and DNA-PKcs-deficient mouse embryonic fibroblasts". Chromosoma. 126 (2): 287- ... mice defective in SIRT7 show phenotypic and molecular signs of accelerated aging such as premature pronounced curvature of the ... SIRT6-deficient mice develop profound lymphopenia, loss of subcutaneous fat and lordokyphosis, and these defects overlap with ... mice with deficient ERCC5 show loss of subcutaneous fat, kyphosis, osteoporosis, retinal photoreceptor loss, liver aging, ...
"Establishment of a Human Cutaneous T-Cell Lymphoma in C.B-17 SCID Mice". Journal of Investigative Dermatology. 94 (3): 381-384 ...
1985 - Started producing BALB/c mice. 1991 - Became the first commercial provider of the C.B-17 SCID mouse model. 1994 - ... They continue to supply NCI with BDF1 mice. 1975 - Began offering the SHR hypertensive rat and the WKY control strain. 1980 - ... 1949 - Robert Phelan began shipping mice from his garage in Canajoharie, NY. 1952 - Taconic Farms is officially founded in ... 1963 - Contracted to provide BDF1 mice for the National Cancer Institute. 1969 - Began offering rats, including Sprague Dawley ...
KSHV targets multiple leukocyte lineages during long-term productive infection in NOD/SCID mice. Journal of Clinical ...
"DNA repair kinetics in SCID mice Sertoli cells and DNA-PKcs-deficient mouse embryonic fibroblasts". Chromosoma. 126 (2): 287- ... However, in mice there is no increase in mutation in the brain with aging. Mice defective in a gene (Pms2) that ordinarily ... mutant mice defective in Ku70, or Ku80, or double mutant mice deficient in both Ku70 and Ku80 exhibit early aging. The mean ... and the three mutant mice were found to display the same aging signs as the control mice, but at a much earlier age. Cancer ...
"Development of an orthotopic SCID mouse-human tumor xenograft model displaying the multidrug-resistant phenotype". Cancer ... WEINSTEIN, R (1972). "Effects of continuous inhalation of dichloromethane in the mouse: Morphologic and functional observations ...
"Potent activity of the HIV-1 maturation inhibitor bevirimat in SCID-hu Thy/Liv mice". PLoS ONE. 2 (11): e1251. doi:10.1371/ ...
All NHEJ mutant mice show a SCID phenotype, sensitivity to ionizing radiation, and neuronal apoptosis. A system was developed ... NHEJ efficiency could be compared across tissues of the same mouse and in mice of different age. Efficiency was higher in the ... SCID) due to defective V(D)J recombination. Loss-of-function mutations in Artemis also cause SCID, but these patients do not ... Many NHEJ genes have been knocked out in mice. Deletion of XRCC4 or LIG4 causes embryonic lethality in mice, indicating that ...
Myeloma cells overexpressing Sulf1 and 2 were subcutaneously injected in severe combined immunodeficient (SCID) mice. Enhanced ... deficient mice, BMP deficient mice and hypermorphic Fgfr1 and 3 mice. This provides evidence that Sulf1 and 2 is linked to HS ... Sulf null mice and other model systems implicated Sulfs in other developmental and disease systems. For example, studies ... "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. "Entrez Gene: SULF1 ...
1980 Discovery of the SCID mouse, a mouse strain with no natural immunity, by Melvin Bosma. The SCID mouse is an essential ... 1981 Beatrice Mintz's laboratory is one of the first to introduce a cloned gene into fertilized mouse eggs and prove that it is ... 1979 Beatrice Mintz shows that a fatal genetic anemia of mice can be prevented in utero by injecting normal blood-forming stem ... 1993 Beatrice Mintz produces the first mouse model of human malignant melanoma, in which the disease resembles the human ...
... a small molecule inhibitor of Kv1.3 is effective in the SCID mouse psoriasis--xenograft model". Journal of Autoimmunity. 55: 63 ... "The beneficial effect of blocking Kv1.3 in the psoriasiform SCID mouse model". The Journal of Investigative Dermatology. 131 (1 ... In a mouse model of diet-induced obesity, ShK-186 counteracted the negative effects of increased caloric intake. It reduced ... In a mouse model of brain radiation, ShK-170 reversed neurological deficits, and protected neurons from radiation-induced brain ...
"Modeling human lymphoid precursor cell gene therapy in the SCID-hu mouse". Blood. 84 (5): 1393-1398. doi:10.1182/blood.v84.5. ...
"Overexpression of plasminogen activator inhibitor 2 in human melanoma cells inhibits spontaneous metastasis in scid/scid mice" ... "Mouse PubMed Reference:".. *^ Schroder WA, Major L, Suhrbier A (2011). "The role of SerpinB2 in immunity". Critical Reviews in ...
... administration of the antibody prolonged survival of SCID mice implanted with JOK-1 cells. Apoptosis induction appears to be ... In a mouse model of multiple myeloma, tumor metastasis to bone was decreased in CD47-deficient mice compared with wild type ... CD47 knockout mice show reduced recruitment of blood T cells as well as neutrophils and monocytes in areas of inflammation. ... "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. "Entrez Gene: CD47 ...
Both ESC and EpiSC induce teratoma when injected in the test animals (scid mice) which proves pluripotency. EpiSC display ... Scientists have been able to demonstrate the induction of EpiSC-like cells in vitro from mouse ESCs, which are referred to as ... Hanna, J (2010). "Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs". ... is similar to primed state mouse stem cells rather than Naïve state. Table 1. Comparison of Naïve and Primed pluripotent states ...
... nude and SCID) mice". Infect Immun. 60 (8): 3325-31. doi:10.1128/IAI.60.8.3325-3331.1992. PMC 257318. PMID 1639500. Lupo PJ, ... first isolated from the gastric glands of the common mouse.Cryptosporidium does originate in common mice, specifically ... Nov.), of the gastric Glands of the Common Mouse". J Med Res. 23 (3): 487-510.3. PMC 2098948. PMID 19971982. "Cryptosporidium ... Cryptosporidium muris has prevalence in the following species in the following amount: Dairy Cows -68% Feedlot Cows- 80% Mice- ...
Experiments in animal models have shown X-SCID to occur similarly in dogs, but not in mice. Alterations in the immune response ... "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. Russell SM, Keegan ... SCID). Most of these mutations involve changes in one or a few nucleotides (DNA building blocks) in the gene. These changes ...
B-17 scid mice". International Immunology. 5 (11): 1461-71. doi:10.1093/intimm/5.11.1461. PMID 7903159. Powrie F, Leach MW, ... CD45RBhi T cells to Rag deficient or SCID mice led to the development of severe intestinal inflammation and wasting disease. ... Mauze S, Menon S, Caddle LB, Coffman RL (1994). "Inhibition of Th1 responses prevents inflammatory bowel disease in scid mice ... This work identified that CD4+OX22hi (OX22 is CD45RC in rats and the equivalent of CD45RB in mice, both isoforms of CD45) T ...
Severe combined immunodeficiency (SCID) mice models demonstrate that these precursors may be transmitted to recipients with ... Deficit of this factor in mice leads to a state resembling systemic lupus erythematosus (SLE). Furthermore, mice lacking LT or ... In mice lacking B cells, or with blocked TNF-a and lymphotoxin (LT) production, cells with FDC phenotype are missing. In normal ... "Impaired prion replication in spleens of mice lacking functional follicular dendritic cells". Science. 288 (5469): 1257-9. doi: ...
... inhibits epithelial-mesenchymal transition and human prostate cancer stem cell growth in NOD/SCID IL2Rγ null mice by regulating ... 5E1 also restricts the proliferation of pancreatic cancer cells in mice. While shown to be effective in the lab, both these Shh ... 5E1, a monoclonal antibody against Shh, has been shown to inhibit medulloblastoma growth in mouse models. ... Molecular therapy targeting Sonic hedgehog and hepatocyte growth factor signaling in a mouse model of medulloblastoma. ...
... and multilineage engraftment potential from subfractions of human CD34+ cord blood cells transplanted into NOD/SCID mice". ... "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. "Entrez Gene: CD34 ... It is important to mention that Long-Term Haematopoietic Stem Cells (LT-HSCs) in mice and humans are the haematopoietic cells ... Antigens,+CD34 at the US National Library of Medicine Medical Subject Headings (MeSH) Mouse CD Antigen Chart Human CD Antigen ...
Studies of the mouse counterpart suggest that this gene may be involved in the development of urogenital tract and female ... SCID)". Nature. 377 (6544): 65-8. doi:10.1038/377065a0. PMID 7659163. S2CID 4329874. Tashima LS, Hieber AD, Greenwood FC, ... "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. Burkhardt E, Adham ... Nef S, Parada LF (July 1999). "Cryptorchidism in mice mutant for Insl3". Nature Genetics. 22 (3): 295-9. doi:10.1038/10364. ...
The two most common types of genetically modified mice are knockout mice and oncomice. Knockout mice are a type of mouse model ... 17 October 2003). "LMO2-Associated Clonal T Cell Proliferation in Two Patients after Gene Therapy for SCID-X1". Science. 302 ( ... Mouse cells were first transformed in 1979, followed by mouse embryos in 1980. Most of the very first transmutations were ... Genetically modified mice are the most common animal model for transgenic research. Transgenic mice are currently being used to ...
Meanwhile, the Structured Clinical Interview for DSM-IV - Dissociative Disorders (SCID-D) and its second iteration, the SCID-D- ... retrosplenial cortex in mice). These slow oscillations disconnect other brain regions from interacting with the posteromedial ... Draijer N, Boon S (1992). The validation of the DES (Dissociative Experience Scale) against the criterium of the SCID-D ( ... SCID-D)". Journal of Trauma & Dissociation. 22 (1): 19-34. doi:10.1080/15299732.2020.1760169. PMID 32419662. S2CID 218678678. ...
September 2009). "Efficient targeting of a SCID gene by an engineered single-chain homing endonuclease". Nucleic Acids Res. 37 ... in contrast to mouse ES cells, have low rates of HR. The technique has been widely adopted for use in engineering human cell ... can be harnessed to perform precise genome alterations in mice. rAAV mediated genome-editing improves the efficiency of this ... "Knock-in of oncogenic Kras does not transform mouse somatic cells but triggers a transcriptional response that classifies human ...
Furthermore, LEKTI-knockout mice exhibit a phenotype similar to Netherton syndrome in humans. There is no known cure at the ... but are not as vulnerable to opportunistic pathogens as patients with true Natural Killer cell deficiency-type SCID. Netherton ... mice". Genes Dev. 18 (19): 2354-8. doi:10.1101/gad.1232104. PMC 522985. PMID 15466487.CS1 maint: multiple names: authors list ( ... "Spink5-deficient mice mimic Netherton syndrome through degradation of desmoglein 1 by epidermal protease hyperactivity". Nat ...
Doctors perform the first successful bone marrow transplant, to treat severe combined immunodeficiency (SCID). DiGeorge ... Engelbart of Stanford Research Institute's Augmentation Research Center demonstrates for the first time the computer mouse, the ...
... has also shown to reduce Aβ deposits in a transgenic mouse model of Alzeimer's dementia. There is also research going on on ... or even genetic diseases such as SCID. Some gene therapies have already been developed or are being developed to correct these ... PS1 Transgenic Mice". Molecular Therapy. 16 (9): 1580-1586. doi:10.1038/mt.2008.148. PMC 2706523. PMID 18665160. ProQuest ... "Successful Preclinical Development of Gene Therapy for Recombinase-Activating Gene-1-Deficient SCID". Molecular Therapy - ...
2017). "Single Cell-Based Vector Tracing in Patients with ADA-SCID Treated with Stem Cell Gene Therapy". Molecular Therapy - ... 2015). "In vivo genome editing improves muscle function in a mouse model of Duchenne muscular dystrophy". Science. 351 (6271): ... and RT-ddPCR protocols for mouse studies". Methods. doi:10.1016/j.ymeth.2020.07.004. ISSN 1046-2023. Taylor, Sean S.; et al. ( ...
The transgenic mice were not susceptible to HBV, but mice with severe combined immunodeficiency (SCID) can develop chronic ... Mice: Several researchers developed transgenic mice in order to study the expression of coding regions for the surface antigens ... In addition, they permitted HBV to replicate in human hepatocytes that were able to proliferate in these mice. In general, ...
... which was then xenografted into SCID mice and later harvested and plated on tissue culture dishes, where it can be propagated ... VCaP cells are XMRV virus positive and produce the mouse xenotropic retrovirus Bxv-1, likely acquired during passaging in ... infected mice. Korenchuk, S; Lehr, JE; MClean, L; Lee, YG; Whitney, S; Vessella, R; Lin, DL; Pienta, KJ (2001). "VCaP, a cell- ...
January 2007). "WHIM syndrome myelokathexis reproduced in the NOD/SCID mouse xenotransplant model engrafted with healthy human ...
Jiang HQ, Kushnir N, Thurnheer MC, Bos NA, Cebra JJ (May 2002). "Monoassociation of SCID mice with Helicobacter muridarum, but ... Feng, S.; Ku, K.; Hodzic, E.; Lorenzana, E.; Freet, K.; Barthold, S. W. (2005). "Differential Detection of Five Mouse-Infecting ... with Enterohepatic Helicobacter Species Can Ameliorate or Promote Helicobacter pylori-Induced Gastric Pathology in C57BL/6 Mice ...
Changes in the proliferative activity of human hematopoietic stem cells in NOD/SCID mice and enhancement of their ... rather than in mice. He received this further clinical training in Toronto and Vancouver, becoming a Fellow of the Royal ... a gene activated by insertional mutagenesis in mouse models of leukemia. Blood 103: 3897-3904, 2004. Leukemia/Bone Marrow ...
... mice, rats, rabbits, pigs, cattle, and various types of mammalian cells. Zinc finger nucleases have also been used in a mouse ... September 2009). "Efficient targeting of a SCID gene by an engineered single-chain homing endonuclease". Nucleic Acids Res. 37 ... "In vivo genome editing restores haemostasis in a mouse model of haemophilia". Nature. 475 (7355): 217-221. doi:10.1038/ ...
In vivo models have demonstrated NSG (NOD/SCID/γ-chain deficient mice) with defects of both lymphocyte and myeloid lineage ... An in vivo model found that GM-CSF knockout CAR-T cells do not induce CRS in mice. However, IL-1 knockout and IL-6 knockout ... Monoclonal antibody blockade of GM-CSF with lenzilumab has been demonstrated to protect mice from CAR-T associated CRS and ... December 2016). "Mechanisms of Acute Toxicity in NKG2D Chimeric Antigen Receptor T Cell-Treated Mice". Journal of Immunology. ...
RAG-2 deficient mice were chosen over other methods of inducting immunodeficiency (such as SCID mice) as an absence of these ... mice although levels of macrophages at the site of tumor challenge are similar to wild type mice. INOS −/− mice also show ... IFN-γ null mice showed virtually no immunity, while IL-4 null mice showed a 50% reduction when compared to immunised wild type ... Hung, 1998) These mice also show reduced antitumor immunity, suggesting that IL-4 deficient mice, which would produce less IL-5 ...
15 December - Scientists use a new form of gene therapy to partially reverse aging in mice. After six weeks of treatment, the ... "Strimvelis receives European marketing authorisation to treat very rare disease, ADA-SCID". GlaxoSmithKline. 27 May 2016. ... "Researchers watch in 3-D as neurons talk to each other in a living mouse brain". Science Daily. 1 November 2016. Retrieved 1 ... Researchers at the University of Toronto use stem cell therapy to reverse age-related osteoporosis in mice. 21 March - Man-made ...
... orthotopic growth and the production of bloody pleural effusion by human malignant pleural mesothelioma cells in SCID mice". ... "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. Nishioka M, Kohno T ...
In Vivo Cell Tracking of Indocyanine Green Labeled Human Stem Cells Transplanted at Superficial or In-Depth Tissue of SCID Mice ... The intravenous LD50 values measured in animals are 60 mg/kg in mice and 87 mg/kg in rats. Occasionally - in one out of 42,000 ...
... mice, quaking MeSH B01.150.900.649.865.635.505.500.550.780 - mice, scid MeSH B01.150.900.649.865.635.505.500.800 - mice, ... mice, inbred a MeSH B01.050.157.520.318 - mice, inbred akr MeSH B01.050.157.520.338 - mice, inbred balb c MeSH B01.050.157.520. ... mice, inbred a MeSH B01.050.199.520.520.318 - mice, inbred akr MeSH B01.050.199.520.520.338 - mice, inbred balb c MeSH B01.050. ... mice, inbred mdx MeSH B01.050.157.520.440 - mice, inbred cba MeSH B01.050.157.520.445 - mice, inbred cftr MeSH B01.050.157.520. ...
S1P has protected ovarian tissue xenografts in SCID mouse models from radiation induced atresia. In animal models these ... Fukuda, Yu; Kihara, Akio; Igarashi, Yasuyuki (12 September 2003). "Distribution of sphingosine kinase activity in mouse tissues ... September 2002). "Sphingosine 1-phosphate preserves fertility in irradiated female mice without propagating genomic damage in ... September 2006). "Sphingosine-1 phosphate prevents monocyte/endothelial interactions in type 1 diabetic NOD mice through ...
Functional defects in this protein may be associated with the pathogenesis of severe combined immunodeficiency (SCID). Several ... Knockout studies in mice suggest that blocking apoptosis is an essential function of this protein during differentiation and ...
Another one is ChAd-SARS-CoV-2-S; the vaccine reportedly prevented mice that were genetically modified to have human ACE2 ( ... This therapy has been found especially useful in treating monogenic disease (e.g. cystic fibrosis, X-linked SCID, alpha1- ...
Thymomagenesis in the NOD-scid/scid mouse was associated with expression of an NOD mouse-unique endogenous ecotropic murine ... The nonobese diabetic scid mouse: model for spontaneous thymomagenesis associated with immunodeficiency.. M Prochazka, H R ... The nonobese diabetic scid mouse: model for spontaneous thymomagenesis associated with immunodeficiency. ... The nonobese diabetic scid mouse: model for spontaneous thymomagenesis associated with immunodeficiency. ...
... including immunodeficient mice such as the Fox Chase SCID Beige mouse, for use in basic research and nonclinical drug discovery ... This mouse was developed by Croy, et al. at the University of Guelph by an intercross of C.B-17 SCID/SCID to C57BL/6 bg/bg mice ... This mouse was developed by Croy, et al. at the University of Guelph by an intercross of C.B-17 SCID/SCID to C57BL/6 bg/bg mice ... This mouse was developed by Croy, et al. at the University of Guelph by an intercross of C.B-17 SCID/SCID to C57BL/6 bg/bg mice ...
Biological context of Mice, SCID. *These results suggest that the SCID-hu mouse provides a useful small animal model to assess ... High impact information on Mice, SCID. *The SCID-hu mouse is a heterochimeric small animal model designed to support ... scid mice [21].. *Significantly more of the HIV-1-infected SCID-hu mice expressed mRNA for human tumor necrosis factors alpha ... Because mice are not able to metabolize thalidomide efficiently, SCID-hu mice received implants of fetal human liver fragments ...
... mouse recipients of congenic splenocytes. The H. pylori-infected and uninfected C57BL/6J and recipient SCID mice were evaluated ... Adoptive Transfer of Splenocytes in SCID Mice Implicates CD4+ T Cells in Apoptosis and Epithelial Proliferation Associated with ... Recipient mice differed, however, according to the source of the transferred CD4+ cells. The CD4+ cell scores for recipients of ... In all H. pylori-infected mice, gastric CD4+ cell scores were increased, compared with scores for uninfected controls. ...
... viral spread was detected by polymerase chain reaction in such mice. Thus, the SCID-hu mouse provides a means to directly ... The SCID-hu mouse, engrafted with human hematolymphoid organs, is permissive for infection with the human immunodeficiency ... However, AZT-treated SCID-hu mice did have a few infected cells; after AZT treatment was stopped, ... Two weeks after infection with HIV, 100 percent (40/40) of SCID-hu mice were positive for HIV by the polymerase chain reaction ...
ICR scid mice are an immunodeficient strain comparable to the C.B-17 scid, but with reduced incidence of Ig production. ... The ICR scid spontaneous mutant model was developed by the Fox Chase Cancer Center by intercrossing ICR mice to C.B-17-SCID ... Mice homozygous for the scid mutation lack both T and B cells due to a defect in V(D)J recombination. They easily accepts ... Scid mice are also useful for examining the relationship between immunity and disease. ...
... Nature. 1994 Feb 17;367(6464):645-8. doi: ... We have now identified an AML-initiating cell by transplantation into severe combined immune-deficient (SCID) mice. These cells ... cells on the basis of cell-surface-marker expression and found that the leukaemia-initiating cells that could engraft SCID mice ...
Severe combined immunodeficient (SCID) mice reconstituted with human peripheral blood leukocytes (hu-PBL-SCID mice) have ... Human immunodeficiency virus infection of human-PBL-SCID mice. By DE Mosier, RJ Gulizia, SM Baird, DB Wilson, DH Spector, SA ... Human immunodeficiency virus infection of human-PBL-SCID mice. By DE Mosier, RJ Gulizia, SM Baird, DB Wilson, DH Spector, SA ... Hu-PBL-SCID mice infected with human immunodeficiency virus-1 (HIV-1) contained virus that was recoverable by culture from the ...
... antibodies to Borrelia burgdorferi-infected C3H/HeN-scid mice increased the severity of acute Lyme arthri ... These data suggest that downregulation of innate immunity in SCID mice in the absence of B- and T-cell responses leads to an ... The administration of interleukin-12 (IL-12) antibodies to Borrelia burgdorferi-infected C3H/HeN-scid mice increased the ...
EBs per mouse) into NOD/SCID/IL2R. mice that were bled (500 L) the day before (Supplemental Figure 3). Mice were sacrificed at ... NOD/SCID, and the improved NOD/SCID/IL2R. mice, lack cell mediated and immunoglobulin-mediated humoral responses (these mice do ... mice (4 mice per group) were used. NOD/SCID/IL2R. mice were anaesthetized with isoflurane (Baxter, Deerfield, USA) and ... Mice. 12-week-old female NOD/SCID/IL. mice were purchased from Jackson laboratory and housed in the animal facility of Memorial ...
SUNY Upstate Medical University currently maintains a severe combined immunodeficiency (SCID) mouse facility which has been ...
To address this issue, 3-wk-old NOD mice received two injections of purified rat brain GAD; one mouse rapidly developed ... Adoptive transfer of 5A cells into NOD/SCID mice produced insulitis in all mice. Diabetes occurred in 83% of the mice. We ... GAD-reactive CD4+ Th1 cells induce diabetes in NOD/SCID mice J Clin Invest. 1998 Jan 1;101(1):68-73. doi: 10.1172/JCI119878. ... To address this issue, 3-wk-old NOD mice received two injections of purified rat brain GAD; one mouse rapidly developed ...
C.B-17 severe combined immunodeficient (scid) mice carry the scid mutation and are severely deficient in both T cell- and B ... scid mutation in mice confers hypersensitivity to ionizing radiation and a deficiency in DNA double-strand break repair.. K A ... scid mutation in mice confers hypersensitivity to ionizing radiation and a deficiency in DNA double-strand break repair. ... scid mutation in mice confers hypersensitivity to ionizing radiation and a deficiency in DNA double-strand break repair. ...
Studies Using Scid Mice.- Growth of Human Tumors in Immune-Deficient scid Mice and nude Mice. ... T Cell-Independent Macrophage Activation in Scid Mice.- Pneumocystis carinii Pneumonia in scid/scid Mice.- Immunobiology of ... Leaky Phenotypes.- T Cell Leakiness in Scid Mice.- Limited Clonal Diversity of Serum Immunoglobulin in Leaky Scid Mice.- ... scid) mouse discovered. Although it has taken most animal facilities several years to breed scid mice of high quality for ...
... infected NOD/SCID mice (hu-SCID) and nonreconstituted, infected NOD/SCID mice (SCID) on day 8. Virus positive-strand RNA was ... Signs of dengue virus infection in humanized NOD/SCID mice. (a) Thrombocytopenia and erythema in infected mice by day 8 (means ... Dengue Fever in Humanized NOD/SCID Mice. Dennis A. Bente, Michael W. Melkus, J. Victor Garcia, Rebeca Rico-Hesse ... The nonreconstituted, infected mouse is on the left, and the reconstituted, infected mouse is on the right (both day 7 ...
Until recently, testing of new therapeutic agents has relied extensively upon the use of mice and nonhuman primates for in vivo ... C.B‐17 scid/scid (SCID) or C.B‐17 scid/scid bg/bg (SCID.BG) mice, preferably male, 6 to 8 weeks old (Taconic Farms) ... SCID‐hu mice). The describes the surgical implantation of human fetal thymus and liver under the kidney capsules of SCID mice ( ... SCID‐hu Thy/Liv model). Subcutaneous transplantation of human fetal bone marrow and thymus (SCID‐hu Bm/Thy mice) is described ...
Single human B cells were sorted from bone marrow and periphery of humanized NOD/SCID γc(null) (hNSG) mice at 8-10 months post ... Human B-cell ontogeny in humanized NOD/SCID γc(null) mice generates a diverse yet auto/poly- and HIV-1-reactive antibody ... Sorting criteria of human B cell subsets from humanized NOD/SCID γcnull (hNSG) mice. (a) Single cell suspension was prepared ... Human B-cell ontogeny in humanized NOD/SCID γcnull mice generates a diverse yet auto/poly- and HIV-1 reactive antibody ...
... in the humanized SCID mouse (hu-PBL-SCID).. METHODS: Two models of hu-PBL-SCID were used for these studies. In one model, human ... The allogeneic response to cultured human skin equivalent in the hu-PBL-SCID mouse model of skin rejection.. Briscoe DM1, ... Both human foreskin and HSE successfully engrafted onto naive SCID mice and remained stable for more than 6 months. In contrast ... HSE also engrafted onto hu-PBL-SCID mice that were exposed to alloantigen by prior injection with interferon-gamma-treated ...
... Ming- ... "Apoptosis Induction in Primary Human Colorectal Cancer Cell Lines and Retarded Tumor Growth in SCID Mice by Sulforaphane," ...
Mice.SCID (C.B-17/Icr-scid/scid) mice and immunocompetent C.B-17 (C.B-17/Icr-+/+) mice were obtained from Japan CLEA Inc. ( ... As controls, SCID mice (n = 10), C.B-17 mice (n = 6), and A/J mice (n = 6) were mock inoculated with PBS. The mice were ... As controls, SCID mice (n = 6) and C.B-17 mice (n = 6) were mock inoculated with PBS. The SCID mice were observed for 60 days, ... The 50% lethal dose of C. burnetii in SCID mice was at least 108 times less than that in immunocompetent mice. The SCID mouse ...
Visualization of the human CD4{sup +} T-cell response in humanized HLA-DR4-expressing NOD/Shi-scid/γc{sup null} (NOG) mice by ... Title: Hepatitis C virus dynamics and cellular gene expression in uPA-SCID chimeric mice with humanized livers during ... Accepted Manuscript: Hepatitis C virus dynamics and cellular gene expression in uPA-SCID chimeric mice with humanized livers ... Hepatitis C virus dynamics and cellular gene expression in uPA-SCID chimeric mice with humanized livers during intravenous ...
Seven cord blood (CB) units were tested for their capacity to repopulate irradiated NOD/SCID mice after one or two successive ... Multilineage engraftment of refrozen cord blood hematopoietic progenitors in NOD/SCID mice. Haematologica 2006;91(3):369-372; ... Multilineage engraftment of refrozen cord blood hematopoietic progenitors in NOD/SCID mice ...
SCID) mice. The drug was administered orally at a dose of 3, 10 and 30 mg/kg, starting shortly before experimental infection of ... the mice. A dose dependent inhibition of arthritic joint swelling was observed. Full protection was obtained with 30 mg/kg ... but progression and severity of the disease was considerably less than in the other treated as well as in the untreated mice. ... Prednisolone reduces experimental arthritis, and inflammatory tissue destruction in SCID mice infected with Borrelia ...
Thymic selection of the human T cell receptor V beta repertoire in SCID-hu mice.. B A Vandekerckhove, R Baccala, D Jones, D H ... Implantation of pieces of human fetal liver and thymus into SCID mice results in the development of a human thymus-like organ, ... Thymic selection of the human T cell receptor V beta repertoire in SCID-hu mice. ... Thymic selection in SCID-hu thymus induces changes in V beta usage by the single-positive CD4+ or CD8+ T cells comparable with ...
... 0-9. A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P. Q. R. S. ...
Mice. C.B-17(H-2d) SCID mice homozygous for the scid mutation were bred from stocks obtained from the SCID Mouse Core Facility ... BALB/c × C57BL/6 F1 (CB6F1) mice, which have the H-2d/H-2b haplotype, were used for transfer into C.B-17 SCID mice. When ... Mice in this colony, including the rederived C.B-17 SCID mice, are devoid of intestinal aerobes. They have been reassociated ... Lymph node lymphocytes prepared from CB6F1 mice were transferred to C.B-17 SCID mice. Eight to ten weeks post-transfer, SI-IEL ...
Lymphomagenesis in SCID-X1 mice following lentivirus-mediated phenotype correction independent of insertional mutagenesis and ... Lymphomagenesis in SCID-X1 mice following lentivirus-mediated phenotype correction independent of insertional mutagenesis and ... "Lymphomagenesis in SCID-X1 Mice Following Lentivirus-mediated Phenotype Correction Independent of Insertional Mutagenesis and ... Lymphomagenesis in SCID-X1 mice following lentivirus-mediated phenotype correction independent of insertional mutagenesis and ...
Human T-cell engraftment in Hu-NOD/SCID mice.. Human CD45+ PBMC engraftment in NOD/SCID mice recipients of allogeneic islet ... ILT3-Fc treatment prevents islet allograft rejection in hu-NOD/SCID mice. Diabetic NOD/SCID mice were transplanted under the ... hu-NOD/SCID mice).. RESEARCH DESIGN AND METHODS. NOD/SCID female mice purchased from Charles River Laboratories were used at 6- ... Ts cell assays in ILT3-Fc-treated (▵) and human IgG-treated (▪) hu-NOD/SCID mice recipients of allogeneic islet cells. Mice ...
Thus, the total cell number in colitic mice ranged from 2 to 11 × 106, whereas MLN from unrestored SCID mice or SCID mice ... Although these activated DC were present in significantly reduced number in SCID mice compared with SCID mice restored with ... protected SCID), normal BALB/c mice, and unreconstituted SCID mice (Fig. 5⇓A). In all cases, the CD134L+ cells were CD11c ... 106 per mouse from protected or unreconstituted SCID mice. Data represent the mean plus SEM of 5-11 mice per group. B, MLN from ...

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