Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.
A mutant strain of Rattus norvegicus without a thymus and with depressed or absent T-cell function. This strain of rats may have a small amount of hair at times, but then lose it.
In vivo methods of screening investigative anticancer drugs, biologic response modifiers or radiotherapies. Human tumor tissue or cells are transplanted into mice or rats followed by tumor treatment regimens. A variety of outcomes are monitored to assess antitumor effectiveness.
A cell line derived from cultured tumor cells.
Experimentally induced new abnormal growth of TISSUES in animals to provide models for studying human neoplasms.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
Substances that inhibit or prevent the proliferation of NEOPLASMS.
A pathologic process consisting of the proliferation of blood vessels in abnormal tissues or in abnormal positions.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.
The transfer of a neoplasm from one organ or part of the body to another remote from the primary site.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
The total amount (cell number, weight, size or volume) of tumor cells or tissue in the body.
Tumors or cancer of the LUNG.
Techniques and strategies which include the use of coding sequences and other conventional or radical means to transform or modify cells for the purpose of treating or reversing disease conditions.
Antibodies produced by a single clone of cells.
Radiotherapy where cytotoxic radionuclides are linked to antibodies in order to deliver toxins directly to tumor targets. Therapy with targeted radiation rather than antibody-targeted toxins (IMMUNOTOXINS) has the advantage that adjacent tumor cells, which lack the appropriate antigenic determinants, can be destroyed by radiation cross-fire. Radioimmunotherapy is sometimes called targeted radiotherapy, but this latter term can also refer to radionuclides linked to non-immune molecules (see RADIOTHERAPY).
Established cell cultures that have the potential to propagate indefinitely.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.
A malignant epithelial tumor with a glandular organization.
A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445)
Unstable isotopes of iodine that decay or disintegrate emitting radiation. I atoms with atomic weights 117-139, except I 127, are radioactive iodine isotopes.
Tumors or cancer of the human BREAST.
Tumors or cancer of the PROSTATE.
Tumors or cancer of the PANCREAS. Depending on the types of ISLET CELLS present in the tumors, various hormones can be secreted: GLUCAGON from PANCREATIC ALPHA CELLS; INSULIN from PANCREATIC BETA CELLS; and SOMATOSTATIN from the SOMATOSTATIN-SECRETING CELLS. Most are malignant except the insulin-producing tumors (INSULINOMA).
A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.
Semisynthetic conjugates of various toxic molecules, including RADIOACTIVE ISOTOPES and bacterial or plant toxins, with specific immune substances such as IMMUNOGLOBULINS; MONOCLONAL ANTIBODIES; and ANTIGENS. The antitumor or antiviral immune substance carries the toxin to the tumor or infected cell where the toxin exerts its poisonous effect.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Ability of neoplasms to infiltrate and actively destroy surrounding tissue.
Tumors or cancer of the LIVER.
A malignant neoplasm made up of epithelial cells tending to infiltrate the surrounding tissues and give rise to metastases. It is a histological type of neoplasm but is often wrongly used as a synonym for "cancer." (From Dorland, 27th ed)
Mapping of the KARYOTYPE of a cell.
Processes required for CELL ENLARGEMENT and CELL PROLIFERATION.
An encapsulated lymphatic organ through which venous blood filters.
Methods of investigating the effectiveness of anticancer cytotoxic drugs and biologic inhibitors. These include in vitro cell-kill models and cytostatic dye exclusion tests as well as in vivo measurement of tumor growth parameters in laboratory animals.
The original member of the family of endothelial cell growth factors referred to as VASCULAR ENDOTHELIAL GROWTH FACTORS. Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as "tumor angiogenesis factor" and "vascular permeability factor". Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating VASODILATION via NITRIC OXIDE-dependent pathways. Alternative splicing of the mRNA for vascular endothelial growth factor A results in several isoforms of the protein being produced.
Tumors or cancer of the OVARY. These neoplasms can be benign or malignant. They are classified according to the tissue of origin, such as the surface EPITHELIUM, the stromal endocrine cells, and the totipotent GERM CELLS.
Experimentally induced tumors of the LIVER.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Elements of limited time intervals, contributing to particular results or situations.
Use of radiolabeled antibodies for diagnostic imaging of neoplasms. Antitumor antibodies are labeled with diverse radionuclides including iodine-131, iodine-123, indium-111, or technetium-99m and injected into the patient. Images are obtained by a scintillation camera.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
A primary malignant neoplasm of epithelial liver cells. It ranges from a well-differentiated tumor with EPITHELIAL CELLS indistinguishable from normal HEPATOCYTES to a poorly differentiated neoplasm. The cells may be uniform or markedly pleomorphic, or form GIANT CELLS. Several classification schemes have been suggested.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Unstable isotopes of indium that decay or disintegrate emitting radiation. In atoms with atomic weights 106-112, 113m, 114, and 116-124 are radioactive indium isotopes.
Experimentally induced mammary neoplasms in animals to provide a model for studying human BREAST NEOPLASMS.
A carcinoma derived from stratified SQUAMOUS EPITHELIAL CELLS. It may also occur in sites where glandular or columnar epithelium is normally present. (From Stedman, 25th ed)
A cytologic technique for measuring the functional capacity of tumor stem cells by assaying their activity. It is used primarily for the in vitro testing of antineoplastic agents.
Benign and malignant central nervous system neoplasms derived from glial cells (i.e., astrocytes, oligodendrocytes, and ependymocytes). Astrocytes may give rise to astrocytomas (ASTROCYTOMA) or glioblastoma multiforme (see GLIOBLASTOMA). Oligodendrocytes give rise to oligodendrogliomas (OLIGODENDROGLIOMA) and ependymocytes may undergo transformation to become EPENDYMOMA; CHOROID PLEXUS NEOPLASMS; or colloid cysts of the third ventricle. (From Escourolle et al., Manual of Basic Neuropathology, 2nd ed, p21)
Unstable isotopes of yttrium that decay or disintegrate emitting radiation. Y atoms with atomic weights 82-88 and 90-96 are radioactive yttrium isotopes.
A sarcoma derived from deep fibrous tissue, characterized by bundles of immature proliferating fibroblasts with variable collagen formation, which tends to invade locally and metastasize by the bloodstream. (Stedman, 25th ed)
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
The grafting of skin in humans or animals from one site to another to replace a lost portion of the body surface skin.
Agents and endogenous substances that antagonize or inhibit the development of new blood vessels.
Rhenium. A metal, atomic number 75, atomic weight 186.2, symbol Re. (Dorland, 28th ed)
The practice of living unclothed for reasons of comfort or health.
Isotopes that exhibit radioactivity and undergo radioactive decay. (From Grant & Hackh's Chemical Dictionary, 5th ed & McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The action of a drug in promoting or enhancing the effectiveness of another drug.
Neoplasms of the intracranial components of the central nervous system, including the cerebral hemispheres, basal ganglia, hypothalamus, thalamus, brain stem, and cerebellum. Brain neoplasms are subdivided into primary (originating from brain tissue) and secondary (i.e., metastatic) forms. Primary neoplasms are subdivided into benign and malignant forms. In general, brain tumors may also be classified by age of onset, histologic type, or presenting location in the brain.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells such as the GOBLET CELLS.
A malignant form of astrocytoma histologically characterized by pleomorphism of cells, nuclear atypia, microhemorrhage, and necrosis. They may arise in any region of the central nervous system, with a predilection for the cerebral hemispheres, basal ganglia, and commissural pathways. Clinical presentation most frequently occurs in the fifth or sixth decade of life with focal neurologic signs or seizures.
Tissues, cells or organs transplanted between animals of different species.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Family of retrovirus-associated DNA sequences (ras) originally isolated from Harvey (H-ras, Ha-ras, rasH) and Kirsten (K-ras, Ki-ras, rasK) murine sarcoma viruses. Ras genes are widely conserved among animal species and sequences corresponding to both H-ras and K-ras genes have been detected in human, avian, murine, and non-vertebrate genomes. The closely related N-ras gene has been detected in human neuroblastoma and sarcoma cell lines. All genes of the family have a similar exon-intron structure and each encodes a p21 protein.

Classification of human colorectal adenocarcinoma cell lines. (1/17429)

Eleven human colorectal adenocarcinoma cell lines established in this laboratory were classified into three groups based on morphological features (light and electron microscopy), modal chromosome number, and ability to synthesize carcinoembryonic antigen (CEA). Group 1 cell lines contained both dedifferentiated and differentiating cells growing in tight clusters or islands of epithelium-like cells; their modal chromosome number was about 47, and they synthesized small to moderate amounts of CEA. Group 2 cell lines were more dedifferentiated, were hyperdiploid, and synthesized small amounts of CEA. Group 3 cell lines were morphologically similar to those of Group 1 by light microscopy. They differed ultrastructurally by containing microvesicular bodies; the modal chromosome number varied from hyperdiploid to hypertriploid or they had bimodal populations of hypodiploid and hypertriploid cells, and they synthesized relatively large amounts of CEA. No correlation could be found between Broder's grade or Duke's classification of the original tumor and modal chromosome number or ability to synthesize CEA. These findings support Nowell's hypothesis that the stem line is different for each solid tumor, which makes it difficult to relate chromosomal changes to the initiation of the neoplastic state.  (+info)

Concomitant activation of pathways downstream of Grb2 and PI 3-kinase is required for MET-mediated metastasis. (2/17429)

The Met tyrosine kinase - the HGF receptor - induces cell transformation and metastasis when constitutively activated. Met signaling is mediated by phosphorylation of two carboxy-terminal tyrosines which act as docking sites for a number of SH2-containing molecules. These include Grb2 and p85 which couple the receptor, respectively, with Ras and PI 3-kinase. We previously showed that a Met mutant designed to obtain preferential coupling with Grb2 (Met2xGrb2) is permissive for motility, increases transformation, but - surprisingly - is impaired in causing invasion and metastasis. In this work we used Met mutants optimized for binding either p85 alone (Met2xPI3K) or p85 and Grb2 (MetPI3K/Grb2) to evaluate the relative importance of Ras and PI 3-kinase as downstream effectors of Met. Met2xPI3K was competent in eliciting motility, but not transformation, invasion, or metastasis. Conversely, MetP13K/Grb2 induced motility, transformation, invasion and metastasis as efficiently as wild type Met. Furthermore, the expression of constitutively active PI 3-kinase in cells transformed by the Met2xGrb2 mutant, fully rescued their ability to invade and metastasize. These data point to a central role for PI 3-kinase in Met-mediated invasiveness, and indicate that simultaneous activation of Ras and PI 3-kinase is required to unleash the Met metastatic potential.  (+info)

11q23.1 and 11q25-qter YACs suppress tumour growth in vivo. (3/17429)

Frequent allelic deletion at chromosome 11q22-q23.1 has been described in breast cancer and a number of other malignancies, suggesting putative tumour suppressor gene(s) within the approximately 8 Mb deleted region. In addition, we recently described another locus, at the 11q25-qter region, frequently deleted in breast cancer, suggesting additional tumour suppressor gene(s) in this approximately 2 Mb deleted region. An 11q YAC contig was accessed and three YACs, one containing the candidate gene ATM at 11q23.1, and two contiguous YACs (overlapping for approximately 400-600 kb) overlying most of the 11q25 deleted region, were retrofitted with a G418 resistance marker and transfected into murine A9 fibrosarcoma cells. Selected A9 transfectant clones (and control untransfected and 'irrelevant' alphoid YAC transfectant A9 clones) were assayed for in vivo tumorigenicity in athymic female Balb c-nu/nu mice. All the 11q YAC transfectant clones demonstrated significant tumour suppression compared to the control untransfected and 'irrelevant' YAC transfected A9 cells. These results define two discrete tumour suppressor loci on chromosome 11q by functional complementation, one to a approximately 1.2 Mb region on 11q23.1 (containing the ATM locus) and another to a approximately 400-600 kb subterminal region on 11q25-qter.  (+info)

The Jun kinase 2 isoform is preferentially required for epidermal growth factor-induced transformation of human A549 lung carcinoma cells. (4/17429)

We have previously found that epidermal growth factor (EGF) mediates growth through the Jun N-terminal kinase/stress-activated kinase (JNK/SAPK) pathway in A549 human lung carcinoma cells. As observed here, EGF treatment also greatly enhances the tumorigenicity of A549 cells, suggesting an important role for JNK in cancer cell growth (F. Bost, R. McKay, N. Dean, and D. Mercola, J. Biol. Chem. 272:33422-33429, 1997). Several isoforms families of JNK, JNK1, JNK2, and JNK3, have been isolated; they arise from alternative splicing of three different genes and have distinct substrate binding properties. Here we have used specific phosphorothioate oligonucleotides targeted against the two major isoforms, JNK1 and JNK2, to discriminate their roles in EGF-induced transformation. Multiple antisense sequences have been screened, and two high-affinity and specific candidates have been identified. Antisense JNK1 eliminated steady-state mRNA and JNK1 protein expression with a 50% effective concentration (EC50) of <0.1 microM but did not alter JNK2 mRNA or protein levels. Conversely, antisense JNK2 specifically eliminated JNK2 steady-state mRNA and protein expression with an EC50 of 0.1 microM. Antisense JNK1 and antisense JNK2 inhibited by 40 and 70%, respectively, EGF-induced total JNK activity, whereas sense and scrambled-sequence control oligonucleotides had no effect. The elimination of mRNA, protein, and JNK activities lasted 48 and 72 h following a single Lipofectin treatment with antisense JNK1 and JNK2, respectively, indicating sufficient duration for examining the impact of specific elimination on the phenotype. Direct proliferation assays demonstrated that antisense JNK2 inhibited EGF-induced doubling of growth as well as the combination of active antisense oligonucleotides did. EGF treatment also induced colony formation in soft agar. This effect was completely inhibited by antisense JNK2 and combined-antisense treatment but not altered by antisense JNK1 alone. These results show that EGF doubles the proliferation (growth in soft agar as well as tumorigenicity in athymic mice) of A549 lung carcinoma cells and that the JNK2 isoform but not JNK1 is utilized for mediating the effects of EGF. This study represents the first demonstration of a cellular phenotype regulated by a JNK isoform family, JNK2.  (+info)

Expression and differential regulation of connective tissue growth factor in pancreatic cancer cells. (5/17429)

CTGF is an immediate early growth responsive gene that has been shown to be a downstream mediator of TGFbeta actions in fibroblasts and vascular endothelial cells. In the present study hCTGF was isolated as immediate early target gene of EGF/TGFalpha in human pancreatic cancer cells by suppression hybridization. CTGF transcripts were found in 13/15 pancreatic cancer cell lines incubated with 10% serum. In 3/7 pancreatic cancer cell lines EGF/TGFalpha induced a significant rise of CTGF transcript levels peaking 1-2 h after the start of treatment. TGFbeta increased CTGF transcript levels in 2/7 pancreatic cancer cell lines after 4 h of treatment and this elevation was sustained after 24 h. Only treatment with TGFbeta was accompanied by a parallel induction of collagen type I transcription. 15/19 human pancreatic cancer tissues were shown to overexpress high levels of CTGF transcripts. CTGF transcript levels in pancreatic cancer tissues and nude mouse xenograft tumors showed a good correlation to the degree of fibrosis. In situ hybridization and the nude mouse experiments revealed that in pancreatic cancer tissues, fibroblasts are the predominant site of CTGF transcription, whereas the tumor cells appear to contribute to a lesser extent. We conclude that CTGF may be of paramount importance for the development of the characteristic desmoplastic reaction in pancreatic cancer tissues.  (+info)

Mechanisms related to [18F]fluorodeoxyglucose uptake of human colon cancers transplanted in nude mice. (6/17429)

[18F]Fluorodeoxyglucose ([18F]FDG), a glucose analogue, has been widely used for tumor imaging. To investigate the mechanisms related to [18F]FDG uptake by tumors, an experiment involving nude mice was performed. METHODS: Human colon cancer cell lines SNU-C2A, SNU-C4 and SNU-C5 were transplanted to nude mice. Using immunohistochemical staining and Western blot, the expression of glucose transporter (Glut) isoforms (Glut-1 through -5) in xenografted tumors was analyzed. For the analysis of messenger ribonucleic acid (mRNA) expression, reverse-transcription polymerase chain reaction and Northern blot were used and the enzyme activity of hexokinase in cancer tissues was measured by continuous spectrophotometric rate determination. RESULTS: [18F]FDG uptake in SNU-C4 and SNU-C5 cells was higher than in normal colon cells. Among these cells and xenografted tumors, SNU-C5 showed the highest level of [18F]FDG uptake, followed by SNU-C4 and SNU-C2A. An immunostaining experiment showed intense staining of Glut-1 in SNU-C5 tumors but somewhat faint staining in SNU-C4. SNU-C5 tumors also showed positive staining with Glut-3, although this was not the case with SNU-C2A and SNU-C4. Western blot analysis showed the expression of Glut-1 and Glut-3 in all tumors. Experiments involving Northern blot analysis and reverse-transcription polymerase chain reaction confirmed the overexpression of Glut-1 mRNA in all tumors, with the highest level in SNU-C5. The level of Glut-3 mRNA was also elevated in SNU-C5 tumors but not in SNU-C2A and SNU-C4. The enzyme activity of hexokinase did not vary among different tumors. CONCLUSION: Gluts, especially Glut-1, are responsible for [18F]FDG uptake in a nude mouse model of colon cancer rather than hexokinase activity. Increased numbers of glucose transporters at the plasma membrane of cancer cells is attributed to an increased level of transcripts of glucose transporter genes and may be a cause of increased [18F]FDG uptake, at least in colon cancer tumors.  (+info)

99mTc-labeled vasoactive intestinal peptide receptor agonist: functional studies. (7/17429)

Vasoactive intestinal peptide (VIP) is a naturally occurring 28-amino acid peptide with a wide range of biological activities. Recent reports suggest that VIP receptors are expressed on a variety of malignant tumor cells and that the receptor density is higher than for somatostatin. Our aims were to label VIP with 99mTc--a generator-produced, inexpensive radionuclide that possesses ideal characteristics for scintigraphic imaging--and to evaluate 99mTc-VIP for bioactivity and its ability to detect experimental tumors. METHODS: VIP28 was modified at the carboxy terminus by the addition of four amino acids that provided an N4 configuration for a strong chelation of 99mTc. To eliminate steric hindrance, 4-aminobutyric acid (Aba) was used as a spacer. VIP28 was labeled with 1251, which served as a control. Biological activity of the modified VIP28 agonist (TP3654) was examined in vitro using a cell-binding assay and an opossum internal anal sphincter (IAS) smooth muscle relaxivity assay. Tissue distribution studies were performed at 4 and 24 h after injection, and receptor-blocking assays were also performed in nude mice bearing human colorectal cancer LS174T. Blood clearance was examined in normal Sprague-Dawley rats. RESULTS: The yield of 99mTc-TP3654 was quantitative, and the yields of 125I-VIP and 1251-TP3654 were >90%. All in vitro data strongly suggested that the biological activity of 99mTc-TP3654 agonist was equivalent to that of VIP28. As the time after injection increased, radioactivity in all tissues decreased, except in the receptor-enriched tumor (P = 0.84) and in the lungs (P = 0.78). The tumor uptake (0.23 percentage injected dose per gram of tissue [%ID/g]) was several-fold higher than 125I-VIP (0.06 %ID/g) at 24 h after injection in the similar system. In mice treated with unlabeled VIP or TP3654, the uptake of 99mTc-TP3654 decreased in all VIP receptor-rich tissues except the kidneys. The blood clearance was biphasic; the alpha half-time was 5 min and the beta half-time was approximately 120 min. CONCLUSION: VIP28 was modified and successfully labeled with 99mTc. The results of all in vitro examinations indicated that the biological activity of TP3654 was equivalent to that of native VIP28 and tumor binding was receptor specific.  (+info)

Effect of tumor necrosis factor alpha on vascular resistance, nitric oxide production, and glucose and oxygen consumption in perfused tissue-isolated human melanoma xenografts. (8/17429)

The effect of tumor necrosis factor alpha (TNF-alpha) on vascular resistance, nitric oxide production, and consumption of oxygen and glucose was examined in a perfused tissue-isolated tumor model in nude mice. One experimental group was perfused with heparinized Krebs-Henseleit buffer, a second one was perfused with TNF-alpha (500 microgram/kg) 5 h before perfusion. The vascular resistance increased significantly 5 h after TNF-alpha injection. The increase in vascular resistance did not seem to be mediated by a decrease in tumor nitric oxide production, as determined by perfusate nitrate/nitrite concentrations, but may be due to aggregation of leukocytes, platelets, and erythrocytes and/or endothelial consumption among the three experimental groups. The oxygen consumption was linearly dependent on the amount of available oxygen in the perfusate, whereas the glucose consumption was constant and independent of the glucose delivery rate. The present experiments provide new insights into physiological and metabolic mechanisms of action of TNF- alpha for optimization of future treatment schedules involving TNF-alpha.  (+info)

TY - JOUR. T1 - Orthotopic transplantation of histologically intact clinical specimens of stomach cancer to nude mice. T2 - Correlation of metastatic sites in mouse and individual patient donors. AU - Furukawa, Toshiharu. AU - Kubota, Tetsuro. AU - Watanabe, Masahiko. AU - Kitajima, Masaki. AU - Hoffman, Robert M.. PY - 1993/2/20. Y1 - 1993/2/20. N2 - Fresh surgical specimens derived from 36 patients with advanced stomach cancer were orthotopically transplanted in nude mice using histologically intact tissue. Twenty of 36 patient tumors gave rise to locally growing tumors in the mice. All 20 patients whose stomach tumors resulted in local growth in the nude mice had clinical lymph‐node involvement, whereas 8 of the other 16 patients whose tumors were rejected had lymph‐node involvement. There was a statistical correlation(p ,0.01) between local tumor growth in nude mice and clinical lymph‐node involvement. Of the 20 cases resulting in local growth in the nude mice, 5 had clinical liver ...
BioAssay record AID 710427 submitted by ChEMBL: Binding affinity to TLR2 in human SU.86.86 cells xenografted in Harlan athymic nude mouse tumor assessed as increase in fluorescence at 100 nmol/kg, iv measured up to 24 hrs.
Peloruside A is a microtubule-stabilizing agent isolated from a New Zealand marine sponge. Peloruside prevents growth of a panel of cancer cell lines at low nanomolar concentrations, including cell lines that are resistant to paclitaxel. Three xenograft studies in athymic nu/nu mice were performed to assess the efficacy of peloruside compared with standard anticancer agents such as paclitaxel, docetaxel, and doxorubicin. The first study examined the effect of 5 and 10 mg/kg peloruside (QD×5) on the growth of H460 non-small cell lung cancer xenografts. Peloruside caused tumor growth inhibition (%TGI) of 84% and 95%, respectively, whereas standard treatments with paclitaxel (8 mg/kg, QD×5) and docetaxel (6.3 mg/kg, Q2D×3) were much less effective (%TGI of 50% and 18%, respectively). In a second xenograft study using A549 lung cancer cells and varied schedules of dosing, activity of peloruside was again superior compared with the taxanes with inhibitions ranging from 51% to 74%, compared with ...
METHODS: Eight 6-8 week old female athymic nude mice were randomly selected. ATC cell line ARO cells were injected into the left ventricular cavity of each mouse respectively. Each mouse was imaged using a dedicated small-animal PET/CT scanner after successful injection of [18F]-FDG under deep anesthesia. Pathological examination was carried out to confirm the bone metastases of ATC ...
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Accessible surface proteins of thymus-derived lymphocytes (T-cells) of normal CBA mice and bone-marrow-derived lymphocytes (B-cells) of congenitally athymic nu/nu mice were analysed. The surfaces of lymphocytes were radioiodinated by using the enzyme lactoperoxidase (EC 1.11.1.7), then solubilized either in acid-urea or in the non-ionic detergent Nonidet P-40. These lysates were then precipitated with antisera specific to either immunoglobulin or the θ-alloantigen in order to assess the presence of these surface markers. Comparable amounts of radioactivity in proteins specifically precipitable as immunoglobulin were obtained from T-lymphocytes and B-lymphocytes when the cells were disrupted by acid-urea. This immunoglobulin had mol. wt. approx. 180000 and was composed of light chains and μ-type heavy chains. When radioiodinated lymphocytes were solubilized with Nonidet P-40, 3-4% of radioiodinated high-molecular-weight protein of B-cells consisted of immunoglobulin, a result similar to that ...
Colony counts and light microscopy were used to assess the capacity of Candida albicans to colonize, infect the alimentary tract, and cause disseminated disease in athymic (nu/nu), euthymic (nu/+), beige (bg/bg), black (bg/+), beige athymic (bg/bg nu/nu), or beige euthymic (bg/bg nu/+) germfree mice. The alimentary tracts of all six genotypes of germfree mice were quickly colonized after exposure to yeast-phase C. albicans. Only bg/bg nu/nu mice showed obvious morbidity and mortality after mucosal colonization with C. albicans. Histopathology of C. albicans-colonized immunocompetent (nu/+, bg/+) and singly immunodeficient (nu/nu, bg/bg, bg/bg nu/+) mice showed minimal to moderate mucosal infections, whereas doubly immunodeficient (bg/bg nu/nu) mice showed extensive yeast and hyphal infection of the palate, tongue, esophagus, and stomach. A progressive systemic infection in C. albicans-colonized mice occurred only in bg/bg nu/nu mice 12 to 16 weeks after colonization and mucosal infection. Thus, ...
Toxins. Both PA and LF were expressed and purified as previously described (15, 17).. Cell lines. The human melanoma cell line we chose to use for the s.c. mouse xenograft model was the SK-MEL-28 cell line. SK-MEL-28 cells were purchased from the American Type Culture Collection (Manassas, VA) and grown as recommended in MEM (Eagle) with 2 mmol/L l-glutamine and Earles balanced salt solution adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mmol/L nonessential amino acids, and 1.0 mmol/L sodium pyruvate, 90%; fetal bovine serum, 10%.. Animals. Female athymic nude mice, 8 to 10 weeks old, were purchased from Charles River Laboratories (Wilmington, MA) and maintained in a ventilated rack system. Irradiated food and autoclaved water were provided ad libitum. The mice were allowed to adjust to their environment for 1 week.. PA-to-LF ratio and timing determination. Athymic nude mice were inoculated s.c. with 10 million SK-MEL-28 melanoma cells. At day 9 post-tumor inoculation, animals were then ...
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Stable green fluorescent protein (GFP) expressing BxPC-3 cells (BxPC3-GFP) from Anticancer, Inc., were developed using previously established transfection method [31]. BxPC3 cells were used for the in vivo study because they are compatible with Cetuximab (an Epidermal Growth Factor Receptor (EGFR) inhibitor) based treatment. BxPC3 cells express EGFR and have wild type KRAS (Kirsten ras oncogene homolog). Cells were grown in T-75 tissue culture flasks with RPMI 1640 culture media (Gibco-BRL, Grand Island, NY, USA) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Hyclone, Logan, UT, USA), and maintained in a humidified incubator at 37 â °C, 95% air and 5% carbon dioxide.. Animal model: bilateral BxPC-3 - GFP mouse flank model. The subcutaneous xenograft model was established by injecting 10 million BXPC-3 - GFP cells subcutaneously in the left and right flanks of male athymic (nu/nu) BALB/c mice (Anticancer, Inc., San Diego, CA) [32]. Animals were selected for treatment ...
Prostate cancer (PCa) is the second most common cancer in US men. Metastasis is the final step of tumor progression and remains the primary cause of PCa death. Hence preclinical, orthotopic models of PCa metastasis are necessary to develop new therapeutics against metastatic disease. Yet unlike irrelevant subcutaneous tumor models, the deployment of orthotopic models of cancer metastasis in drug research and development is limited by the inability to longitudinally monitor cancer progression/regression in response to administration of experimental pharmaceuticals. Recently, a nearinfrared fluorescent protein (iRFP) was created for deeper imaging [1]. Imaging prostate tumor growth and lymph node metastasis in nude mice therefore becomes possible using this new fluorescent gene reporter. In this study, we first developed an intensified CCD (ICCD)-based iRFP fluorescence imaging device. Then human PCa PC3 cell lines expressing iRFP gene reporter were orthotopically implanted in male Nu/Nu mice at ...
In this study, we demonstrate that overexpression of IL-13Rα2 chain inhibits tumorigenicity of human breast and pancreatic tumor cells in nude mice. Although there was no difference in growth between vector only transfected cells and IL-13Rα2 chain transfected cells in vitro, in nude mice, tumorigenicity was impaired in IL-13Rα2 chain overexpressing tumors. The presence of inflammatory cells, particularly neutrophils were found to correlate with the lack of tumorigenicity or significant reduction in tumor growth rate. Neutrophils were found to produce IL-13 and IL-13 had modest antitumor effect in IL-13Rα2 overexpressing tumors. Moreover, IL-13Rα2 overexpressing tumor cells produced high level of IL-8 which has been shown to reduce tumorigenicity in several tumor models. To our knowledge this is the first report that implicates cytokine receptor chain in tumorigenesis.. It is of interest to note that stability of IL-13Rα2 chain expression and tumor growth in nude mice correlated extremely ...
Fingerprint Dive into the research topics of AFAP-110 is overexpressed in prostate cancer and contributes to tumorigenic growth by regulating focal contacts. Together they form a unique fingerprint. ...
Ricciardi, castagnoli P.; Lieberman, M; Finn, O; Kaplan, D H.; and Leukemia, L I., Virus in athymic nude mice. (1978). Subject Strain Bibliography 1978. 1049 ...
In this report, we have examined the role of the NT-trk receptor axes in the development and progression of human prostatic carcinoma and PDAC-derived xenografts in nude mice and the consequences of disrupting trk-mediated signaling in these tumor types using a mixture of NT neutralizing antibodies (anti-NGF, anti-BDNF, anti-NT-3, and anti-NT-4/5). We provide evidence that the NT-trk receptor axes are involved in the growth of TSU-Pr1 and PC-3 prostatic carcinoma and AsPC-1 PDAC xenografts. Treatment with the anti-NT antibody mixture inhibited significantly the growth of TSU-Pr1 and AsPC-1 tumor xenografts over a 15-day period and was generally well tolerated. In addition, treatment of nude mice bearing PC-3 tumors with the antibody mixture (400 μg) caused significant tumor regression. The mixture of NT neutralizing antibodies did not inhibit the growth of either the SK-OV-3 or CFPAC xenografts, although each cell line expressed the high-affinity NGF receptor, trkA, supporting previous data ...
Reed, N D.; Isaak, D D.; and Jacobson, R H., The use of nude mice in model systems for studies on acquired immunity to parasitic helminths, p. 3-16. In proc. Second internat. Workshop nude mice, univ. Tokyo (1977). Subject Strain Bibliography 1977. 1051 ...
Enumeration and quantification of M. leprae in mouse FPs using conventional assays.BALB/c (gray bars) and athymic nu/nu (black bars) mice were infected in the F
Enumeration and quantification of M. leprae in mouse FPs by molecular assays.BALB/c (gray bars) and athymic nu/nu (black bars) mice were infected in with 3×107
The growth characteristics and metastatic behavior of human tumors growing in athymic nude mice were studied. Human tumor cell lines HEp-2 (carcinoma of larynx) and SW480 (colon carcinoma) were transplanted into athymic nude mice of BALB/c origin. Tumor cells (1 × 106 and 2 × 107) were given either s.c. or i.p.. Following s.c. injection tumors developed rapidly to become easily palpable within 2 weeks forming a s.c. tumor focus surrounded by a thick fibrous capsule. Animals with s.c. transplants were little affected by the growing tumor. At the time they were sacrificed at Day 34 (HEp-2) and 62 (SW480), a large part of the tumor was necrotic. Capsular infiltration and invasion of lymphatic vessels and perineural and perivascular lymphatic spaces were observed. Metastases to regional lymph nodes were seen in animals kept alive for up to 6 months.. Following i.p. transplantation, tumors spread widely in the peritoneal cavity, invaded intraabdominal organs, and metastasized to mediastinal lymph ...
TGF-&#946;s are pleiotropic regulatory proteins that are often highly overexpressed in the microenvironment of advanced tumors. Since nearly all cell types respond to TGF-&#946;, TGF-&#946;s are wellpositioned to integrate cellular responses in the parenchymal and stromal compartments of normal tissues and of tumors, and to mediate cross-talk between the two. Pre-clinical studies have uncovered complex roles for TGF-&#946;s in tumorigenesis, with tumor suppressive and tumor promoting effects on both compartments. In this review, we discuss the biological activities of TGF-&#946; that underlie these effects and address some of the mechanisms that determine which type of effect dominates at different stages of the tumorigenic process. Finally, we consider the prospects for therapeutic targeting of TGF-&#946; in cancer, as a way to simultaneously normalize both the microenvironmental and parenchymal compartments of the tumor, and thus prevent or reverse tumor progression ...
nude mouse definition: a mouse with an inherited problem that prevents all of them from developing locks also stops them from immunologically rejecting personal cells and tissues; popular in preclinical…
4791 Prostate cancer (PCA) remains the most commonly diagnosed visceral cancer in men with more than 230,000 newly diagnosed cases in 2005, and is the second leading cause of cancer deaths among US men. Epidermal growth factor (EGF) and insulin-like growth factor-I (IGF-I) are potent mitogens that regulate proliferation and survival of PCA cells via autocrine and paracrine loops involving MAPK, Akt and NF-κB-mediated signaling pathways. Recently we showed that inositol hexaphosphate (IP6) inhibits human PCA DU145 xenograft growth in male nude mice, which was associated with its anti-proliferative and apoptotic effects in both in vivo and in vitro PCA systems; however, its mechanism of efficacy is not known completely. Here, we performed detailed mechanistic studies with IP6 to assess and define its effect on growth inhibition and apoptosis in human PCA PC3 cells cultured under serum or starved-condition without or with growth factor stimulation. Cell growth and proliferation were assayed by ...
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The T84 cell line is a transplantable human carcinoma cell line derived from a lung metastasis of a colon carcinoma in a 72-year-old male. Tumor tissue was inoculated subcutaneously and serially transplanted in BALB/c nude mice.
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In Vivo: MK-2206 shows 60% TGI and inhibits more than 70 % of phospho-Akt1/2 (T308 and S473) in A2780 ovarian cancer xenografts at a dose of 240 mg/kg. [1] MK-2206 exhibits significant antitumor activity in NCI-H292 xenograft in combination with erlotinib or ...
3. Kiguchi, K., Kubota, T., Aoki, D., Udagawa, Y., Yamanouchi, S., Saga, M., Amemiya, A., Nozawa, S., Moossa, A.R., and Hoffman, R.M. A patient-like orthotopic implantation nude mouse model of highly metastatic human ovarian cancer. Clinical & Experimental Metastasis 16, 751-756, 1998. ...
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SVEC4-10EHR1 is an endothelial cell line derived from the same solid tumor as SVEC4-10EE2 (see ATCC CRL-2167) in a nude mouse injected with SVEC4-10 (see ATCC CRL-2181). SVEC4-10EHR1 was cloned from cells from the SVEC4-10 induced tumor in 1990 by limiting dilution using medium conditioned by SVEC4-10EE2.
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The HBx protein is known to be a promiscuous transactivator of many viral and cellular promoters in HBV-infected cells, including the promoters of genes involved in cell growth regulation, such as c-fos, c-jun, c-myc, and the epidermal growth factor receptor. In addition to these essential roles, the protein is thought to play a role in the development of HCC during chronic infection (4, 5, 6, 7, 8, 9, 10). In HBx transgenic mice, it has been observed that the development of HCC is critically related to the expression level of HBx protein and the expression of HBx increases the sensitivity to the tumorigenic effects of hepatocarcinogens 2- to 3-fold (31). Although the roles of HBx in viral infection and carcinoma have been well elucidated, its role as either an immunogen or cancer Ag in HBV infection is still unclear.. In this present study, we evaluated whether HBx-specific CTLs exist in chronic HBV patients, and if so, whether these cells are capable of recognizing HBx-derived epitopes and can ...
Athymic nude mice recover from an infection with recombinant vaccinia virus (VV) encoding murine interleukin 2 (IL-2), but treatment with a mAb to IL-2 accentuated infection. Administration of a mAb against interferon gamma (IFN-gamma) to mice infected with the IL-2-encoding virus completely prevented the IL-2-induced mechanisms of recovery. Both asialo-GM1+ (NK) and asialo-GM1- (non-NK) cells were participants in the IFN-gamma-mediated recovery of nude mice from infection with the IL-2-encoding VV recombinant. Depletion of asialo-GM1+ cells exacerbated infection, though not as much as anti-IFN-gamma mAb. In vitro, both asialo-GM1+ and asialo-GM1- nude mouse splenocytes produced IFN-gamma in response to IL-2. ...
A mouse model of metastasis of human gastric cancer is one of the most important tools for studying the biological mechanisms underlying human gastric cancer metastasis. In this paper, we established a mouse model of metastatic human gastric cancer in nude mice that has a higher rate of tumor formation and metastasis than existing models. To generate the mouse model of metastatic human gastric cancer, fresh tumor tissues from patients that have undergone surgery for gastric cancer were subcutaneously implanted in the right and left groins of nude mice. When the implanted tissue grew to 1 cubic centimeter, the mice were killed, and the tumor tissues were examined and resected. The tumor tissues were implanted into nude mice and subjected to pathological examination, immunohistochemical staining, and real-time PCR for cytokeratin 8/18 (CK8/18), E-cadherin, vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1). The mice were also analyzed for metastasis in their
A mouse gastric cancer model is an important tool for studying the mechanisms of gastric cancer. To establish subcutaneously implanted tumors, MKN-45 cell suspensions and tumor tissues were implanted into the middle of the right armpit of nude mice. To generate an abdominal metastasis model, MKN-45 cell suspensions and tumor tissue homogenates were implanted into the middle of the lower abdomen. We measured the weights of the nude mice and the longest dimension, shortest dimension, thickness, and volume of the tumor. We also analyzed the rate of tumor formation, the time required for tumor formation, and the number and size of abdominal tumors in the mice. The rates of formation of the subcutaneously implanted tumors were 100%, 0%, and 100% in the nude mice inoculated with 2 × 10|sup|7|/sup| cells/mL or 1 × 10|sup|7|/sup| cells/mL of the MKN-45 cell suspension or the tumor tissue homogenate (2 × 10|sup|7|/sup| cells/mL),
Metastatic uveal melanoma is a highly fatal disease; most patients die from their hepatic metastasis within 1 year. A major drawback in the development of new treatments for metastatic uveal melanoma is the difficulty in obtaining appropriate cell lines and the lack of appropriate animal models. Patient-derived xenograft (PDX) tumor models, bearing ectopically implanted tumors at a subcutaneous site, have been developed. However, these ectopically implanted PDX models have obstacles to translational research, including a low engraftment rate, slow tumor growth, and biological changes after multiple passages due to the different microenvironment. To overcome these limitations, we developed a new method to directly transplant biopsy specimens to the liver of immunocompromised mice. By using two metastatic uveal melanoma cell lines, we demonstrated that the liver provides a more suitable microenvironment for tumor growth compared to subcutaneous sites and that surgical orthotopic implantation (SOI) of
Cyclooxygenase-2 (COX-2) is associated with poor-prognosis breast cancer. We used a nude mouse xenograft model to determine the effects of COX-2 inhibition in breast cancer. Oestrogen receptor (ER)-positive MCF7/HER2-18 and ER-negative MDAMB231 breast cancer cell lines were injected into nude mice and allowed to form tumours. Mice then received either chow containing Celecoxib (a COX-2 inhibitor) or control and tumour growth measured. Tumour proliferation, apoptosis, COX-2, lymphangiogenesis and angiogenesis were assessed by immunohistochemistry (IHC), Western blotting or Q-PCR. Celecoxib inhibited median tumour growth in MCF7/HER2-18 (58.7%, P=0.029) and MDAMB231 (46.3%, P=0.0002) cell lines compared to control. Cyclooxygenase-2 expression decreased following Celecoxib treatment (MCF7/HER2-18 median control 65.3% vs treated 22.5%, P=0.0001). Celecoxib increased apoptosis in MCF7/HER2-18 tumours (TUNEL 0.52% control vs 0.73% treated, P=0.0004) via inactivation of AKT (median pAKT(ser473) 57.3% control
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TY - JOUR. T1 - Antitumor activity of [Pt(O,O-acac)(γ-acac)(DMS)] in mouse xenograft model of breast cancer. AU - Muscella, A.. AU - Vetrugno, C.. AU - Migoni, D.. AU - Biagioni, F.. AU - Fanizzi, F. P.. AU - Fornai, F.. AU - De Pascali, S. A.. AU - Marsigliante, S.. PY - 2014. Y1 - 2014. N2 - The higher and selective cytotoxicity of [Pt(O,O-acac)(γ-acac)(DMS)] toward cancer cell in both immortalized cell lines and in breast cancer cells in primary cultures, stimulated a pre-clinical study so as to evaluate its therapeutic potential in vivo. The efficacy of [Pt(O,O-acac)(γ-acac)(DMS) ] was assessed using a xenograft model of breast cancer developed by injection of MCF-7 cells in the flank of BALB/c nude mice. Treatment of solid tumor-bearing mice with [Pt(O,O-acac)(γ-acac)(DMS)] induced up to 50% reduction of tumor mass compared with an average 10% inhibition recorded in cisplatin-treated animals. Thus, chemotherapy with [Pt(O,O-acac)(γ-acac) (DMS)] was much more effective than ...
Figure 6. A, dietary GSPs inhibit the growth of A549 and H1299 non-small cell lung cancer cells (NSCLC) grown as xenografts in athymic nude mice (29). Tumor xenografts tissues were harvested at the termination of the experiment, and the wet weight of the tumor/mouse in each group is reported in grams as mean ± SD, n = 10. Statistical significance versus non-GSP-treated controls, *, P , 0.05; ¶, P , 0.01; †, P , 0.001. B, tumor xenograft tissues from control and GSP-treated (0.5%, w/w) mice were used for the analysis of the levels of COX-2 and PGE2 receptors using Western blotting. Dietary GSPs inhibit the levels of COX-2 and PGE2 receptors, EP1, EP3 and EP4, in the tumor xenograft tissues grown in athymic nude mice compared with control tumor xenograft tissues. Representative blots from A549 or H1299 xenografts are presented from the independent analysis of tumors from 6 animals per group with identical results. The relative density (arbitrary) of each band after normalization for β-actin ...
TY - JOUR. T1 - Suppression of distant pulmonary metastasis of MDA-MB 435 human breast carcinoma established in mammary fat pads of nude mice by retroviral-mediated TIMP-2 gene transfer. AU - Lee, Young Kwan. AU - So, In Seob. AU - Lee, Sang Chul. AU - Lee, Jeong Hyung. AU - Lee, Chang Woo. AU - Kim, Whan Mook. AU - Park, Mi Kyung. AU - Lee, Seung Taik. AU - Park, Do Youn. AU - Shin, Deug Yong. AU - Park, Chung Ung. AU - Kim, Yeon Soo. PY - 2005/2/1. Y1 - 2005/2/1. N2 - Background: Previous studies have shown that TIMP-2 overexpression is a useful therapeutic tool for inhibiting tumor growth and invasion in animals. However, it has not been reported whether genetic manipulation for TIMP-2 overexpression can induce an inhibitory effect on s0pontaneous metastasis from the primary tumor site to other organs such as lungs or lymph nodes in an animal model. Methods: The present studies describe the effects of retrovirus-mediated TIMP-2 gene transfer into human breast cancer cell lines on the in vitro ...
HCC is one of the deadliest types of cancers, with a mortality of almost 100 % [36]. The mortality of HCC remains high because the disease is typically diagnosed when it is already at an advanced stage, when most potential curative therapies such as resection and transplantation are of limited efficacy. Recent studies reported that different therapies including surgery [5-7], hepatic artery ligation [37], insufficient radiofrequency ablation [38-40], and sublethal heat treatment [41] promoted residual tumor progression. It is especially important to investigate the underlying mechanism. Our research team has developed a safe and reliable method of palliative HCC resection in an orthotopic nude mouse model, and reported that palliative resection enhances metastatic potential of residual HCC in liver [5]. In the present study, using this method, we investigated the precise mechanism mediating this metastasis.. Data from our institution (1958-2008, unpublished) revealed that in HCC patients who ...
We analyzed CP, PP (Peyers patch), and MLN (mesenteric lymph node) cells obtained from 4-week-old athymic (nu/nu) weanling BALB/c mice by flow cytometry (11). All locations had no CD3+ T cells (Fig. 1B) or CD8+ cells, and most CP cells were c-kit+Lin− (Lin; CD3, B220, Mac-1, Gr-1, and TER119) (Fig. 1B), whereas cells from PPs and MLNs (Fig. 1B), and cells from the tissue fragments without CPs, did not have a c-kit+Lin− population. Almost all c-kit+Lin− CP cells were IL-7R+Pgp-1+ (12), 14% of c-kit+Lin− CP cells were CD4+(Fig. 1B), and 68% of c-kit+Lin− CP cells were Thy1+ (Fig. 1B). Four disinct lymphocyte subsets were present among c-kit+Lin− CP cells as determined by the expression of CD4 and Thy1. In the three lymphoid tissues of euthymic nu/+ littermates, only CP cells contained a dominant population (60 to 70%) of c-kit+Lin− cells (12). Thus, CPs differed from PPs and MLNs with respect to lymphoid residents, although they all constitute organized gut-associated lymphoid ...
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In the present study, the antitumor effects of Mel-P15 and the underlying molecular mechanisms of these effects in vivo were investigated. Mel-P15 directly stimulated natural killer (NK) cell cytotoxicity in vitro, which was increased to 55.45% at a 4 µg/ml dose of Mel-P15. In the mouse liver cancer (H22) xenograft mice model, Mel-P15 suppressed tumor growth in vivo; the tumor inhibitory rate was 61.15% following treatment with 2 mg/kg Mel-P15. In addition, the immune response was activated following Mel-P15 treatment. Mel-P15 treatment increased the spleen and thymus indices, promoted splenocyte proliferation, stimulated NK cytotoxicity and upregulated the secretion of cytokines, including interleukin-2, interferon-γ and tumor necrosis factor-α. In addition, the tumor inhibitory effect of Mel-P15 on BEL-7402-bearing nude mice was abrogated by the selective depletion of NK cells via the intraperitoneal injection of an anti-asialo GM-1 antibody ...
In the present study, the antitumor effects of Mel-P15 and the underlying molecular mechanisms of these effects in vivo were investigated. Mel-P15 directly stimulated natural killer (NK) cell cytotoxicity in vitro, which was increased to 55.45% at a 4 µg/ml dose of Mel-P15. In the mouse liver cancer (H22) xenograft mice model, Mel-P15 suppressed tumor growth in vivo; the tumor inhibitory rate was 61.15% following treatment with 2 mg/kg Mel-P15. In addition, the immune response was activated following Mel-P15 treatment. Mel-P15 treatment increased the spleen and thymus indices, promoted splenocyte proliferation, stimulated NK cytotoxicity and upregulated the secretion of cytokines, including interleukin-2, interferon-γ and tumor necrosis factor-α. In addition, the tumor inhibitory effect of Mel-P15 on BEL-7402-bearing nude mice was abrogated by the selective depletion of NK cells via the intraperitoneal injection of an anti-asialo GM-1 antibody ...
This study investigated the homing phenomenon in hepatocellular carcinoma (HCC). The homing specificity of endothelial progenitor cells (EPC) by establishing an orthotopic implantation model in nude mice. EPCs harvested from the marrow cells were separated by density gradient centrifugation. Flu …
In control arm, the tumor grew the fastest within the first couple weeks. As the tumor enlarged, we noticed a decline in growth rate of these tumors. In the control arm, the tumor growth rate was significantly higher in the first 15 days compared to the second 15 days. The tumor volume doubling time changed from 103 h in the first 15 days to 144 h in the second 15 days (P , 0.01). However, in the 2 mg T and 5 mg T arms, the opposite results were found. The data demonstrated that in 2 mg T and 5 mg T arms, the tumor growth rate was significantly lower in the first 15 days compared to the second 15 days, (184 h vs 133 h in 2 mg T arm, P , 0.05, 206 h vs 140 h in 5 mg T arm, P , 0.01). When comparing the growth rates of the control arm and the 2 mg T and 5 mg T arms in the first 15 days, the growth rates were much higher in the control arm (P , 0.01). However, in the second 15 days there was no significant difference in tumor growth rates in the control arm, 2 mg T arm, and 5 mg T arms (Fig. 4). ...
Human tumor xenograft models are used for new drug discovery and development, and are extremely useful tools in oncology. These models can be used for general treatment research and for identifying the details of tumor growth and development. Xenograft models are also widely used for personalized cancer treatment set up and tests, as their cancerous cells are similar to those of the original tumor. Xenograft model techniques allow in vivo tests on human tumors, not on mouse tumors, which may not have the same response on a particular drug or dose concentration.. Studies with xenotransplantation can be used to measure the activity of compounds based on the rate of growth exhibited by the engrafted tumors. Experimental targets may be tumors that originate from specific tissues such as breast, colon or lung. A multitude of tissue specific models exist and more are being developed. Altogen Labs has the expertise to utilize these models and any new models as they become available. In addition, we ...
Gd-albumin is efficiently accumulated in SCC transplants. MRI with low loaded Gd-albumin may offer relevant opportunities for recognizing tumors sensitive to a therapy with cyostic drug-labeled albumins.
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Mouse monoclonal to BLK Keeping track of Package-8 (CCK-8, Beyotime, China) and regular colony development assays had been utilized to measure cell proliferation. Each test was repeated 3-4 moments. For cell routine analysis, cells had been gathered 48 hrs after transfection with indicated plasmids, stained with propidium iodide (PI, Sigma) and assayed utilizing a Beckman Coulter Movement Cytometer (Fullerton). Man BALB/c nude mice (4~6 weeks old) had been purchased from the pet Research Committee from the Institute of KU-0063794 Biology and Cell Biology (Shanghai, China) and housed in the Shandong College or university School of Medication animal facility regarding to protocols accepted by the Shandong College or university Animal Treatment Committee. HepG2.2.15 cells (1107) were transplanted subcutaneously into nude mice. After achieving a size of 0.5 cm, tumors were injected with plasmid (20g/100l) every fourth day for a complete of 3-4 injections. Tumor size was supervised every other time. ...
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Multidrug level of resistance (MDR) is a trend where tumor cells become simultaneously resistant to anticancer medicines with different constructions and systems of actions. telatinib (15 mg/kg) with doxorubicin (1.8 mg/kg) significantly decreased the development price and tumor size of ABCG2 overexpressing tumors inside a xenograft nude mouse magic size. These results, so long as they could be translated to human beings, recommending that telatinib, in conjunction with particular ABCG2 substrate medicines could be useful in dealing with tumors that overexpress ABCG2. gene manifestation in addition has been connected with poor response to chemotherapy in years as a child severe myeloid leukemia (AML) and relapsed AML [13, 14]. Furthermore, increased mRNA continues to be reported in irinotecan treated hepatic metastases than in irinotecan-naive metastases [15]. ABCG2 manifestation continues to be reported in a variety of solid tumors, such as for example digestive system, endometrium and melanoma ...
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Cell-line tumor. Huh-7-GFP cells (2×106) in 0.1 ml phosphate-buffered saline (PBS) were initially subcutaneously injected in the shoulder of nude mice. Resulting tumors were harvested and sectioned into small pieces for sutureless SOI to the liver.. Patient-derived tumor. A colon-cancer liver metastasis from a human patient was previously surgically obtained under standard sterile conditions at the UCSD Thornton hospital under UCSD IRB protocol 140046. Informed patient consent to utilize the tumor tissue for research was obtained prior to surgical resection (Figure 1A). Fresh tumor fragments were subcutaneously implanted in nude mice (Figure 1B). Once tumor growth was established subcutaneously, tumors were harvested and sectioned into small pieces for sutureless SOI to the liver (Figure 1C).. Sutureless SOI to the liver. Nude mice (n=10) were injected intra-muscularly with a ketamine solution (0.02 mL) for anesthesia prior to all procedures. The entire abdomen was sterilized with a 70% ethanol ...
Supplementary Materials? CAS-110-2794-s001. cervical malignancy cells. In vivo, xenograft experiments verified that SALL4 enhanced the tumor formation of cervical malignancy cells in female BALB/c Nude mice. Cell cycle analysis by fluorescence\activated cell sorting found that SALL4 accelerates cell cycle transition from your G0/G1 phase to the S phase. TOP/FOP\Adobe flash reporter assay exposed that SALL4 […]. ...
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Animal models for human tumor xenografts are used for the study of biology and treatment of human cancer. The aim of this study was to develop a model for long-term tumor observations with the...
Athymic Nude MouseIn Vivo Targeting of OV-TL 3 Immunoliposomes to Ascitic Ovarian Carcinoma Cells (OVCAR-3) in Athymic Nude MiceNU/NU Nude Mouse
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The first nude mouse strain is discovered by Dr. N. R. Grist at Ruchill Hospital's Brownlee virology laboratory in Glasgow. ... "NMRI Nude Mice" (PDF). Charles River. Archived from the original (PDF) on 1 October 2011. Retrieved 4 August 2011.. ... ISBN 0 900178 02 7. "Mouse (immunodeficient)". AnimalResearch.info. Archived from the original on 4 September 2011. Retrieved 4 ...
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Zhang Z, Burnley P, Coder B, Su DM (2012). "Insights on FoxN1 biological significance and usages of the "nude" mouse in studies ... Mutations in the winged-helix transcription factor gene at the nude locus in mice and rats produce the pleiotropic phenotype of ... Schorpp M, Hofmann M, Dear TN, Boehm T (Dec 1997). "Characterization of mouse and human nude genes". Immunogenetics. 46 (6): ... "New member of the winged-helix protein family disrupted in mouse and rat nude mutations". Nature. 372 (6501): 103-7. doi: ...
"Naked Jessica Rabbit". Snopes.com. Retrieved July 13, 2009. Schweizer, Peter; Schweizer, Rochelle (1998). Disney: The Mouse ... Mickey Mouse, Minnie Mouse, Tom and Jerry, Heckle and Jeckle, Chip n' Dale, Felix the Cat, Herman and Katnip, Mighty Mouse, ... View topic - Mickey Mouse & Roger Rabbit in The Stooge". Dvdizzy.com. Retrieved August 24, 2014. Brew, Simon. "Who Framed Roger ... Joe Alaskey voiced Yosemite Sam (in place of Blanc), Wayne Allwine voiced Mickey Mouse, Tony Anselmo voiced Donald Duck (also ...
The first nude mice were discovered by Dr. Norman R. Grist at the newly-estblished Brownlee virology laboratory in the hospital ... CS1 maint: discouraged parameter (link) "Nude Mice" (PDF). Archived from the original (PDF) on 2011-10-01. Retrieved 2013-06-08 ...
The first nude mouse strain is discovered by Dr. N. R. Grist at Ruchill Hospital's Brownlee virology laboratory in Glasgow. The ... CS1 maint: discouraged parameter (link) "NMRI Nude Mice" (PDF). Charles River. Archived from the original (PDF) on 2011-10-01. ... "Mouse (immunodeficient)". AnimalResearch.info. Archived from the original on 2011-09-04. Retrieved 2011-08-04. ... including all five of the naked-eye planets plus the Sun and Moon, all within 16° of each another on the ecliptic. February 19 ...
The naked stem gives the plant its common name. Naked buckwheat can be found scattered around the west coast of the United ... murinum - mouse buckwheat Eriogonum nudum var. nudum Eriogonum nudum var. oblongifolium - oblong-leaved buckwheat Eriogonum ... Eriogonum nudum is a perennial shrub of the wild buckwheat genus which is known by the common name naked buckwheat or nude ... The complex of varieties and subspecies of naked buckwheat includes: Eriogonum nudum var. auriculatum - eared naked buckwheat ...
The tails of mouse opossums are naked. Mouse opossums have ears that are, both, large and naked. General opossums, including ... Gracile mouse opossums, excluding the fat-tailed mouse opossum, in general, have a prehensile tail that is thin and very long. ... G. aceramarcae has naked skin compared to G. ignitus. G. emiliae is smaller than G. ignitus. G. emiliae, in general, has a very ... Martins, E. G.; Bonato, V.; Pinheiro, H. P.; dos Reis, S. F. (May 2006). "Diet of the gracile mouse opossum (Gracilinanus ...
March 1992). "Lymphocytic choriomeningitis outbreak associated with nude mice in a research institute". JAMA. 267 (10): 1349-53 ... Although the house mouse (Mus musculus) is the primary reservoir host for LCMV, it is also often found in the wood mouse ( ... Other modes of mouse-to-mouse transmission include nasal secretions, milk from infected dams, bites, and during social grooming ... LCMV is naturally spread by the common house mouse, Mus musculus. Once infected, these mice can become chronically infected by ...
Long-term function of tilapia islet tissue in diabetic nude mice". Diabetes. 41 (12): 1528-1532. doi:10.2337/diab.41.12.1528. ... and fish islet grafts transplanted into diabetic nude mice". General and Comparative Endocrinology. 106 (3): 384-388. doi: ... The tilapia islet grafts give better blood glucose level than rat or mouse islet grafts. But as in mammalian transplant, tissue ... The transplantation of tilapia Brockmann bodies into a diabetic mice model has been shown to promote long-term normal blood ...
Patients were expected to sit inside them naked. The accumulators were tested on plant growth and mice with cancer. Reich wrote ... Children were asked to stand naked in front of Reich and a group of 30 therapists in his basement, while Reich described the ... Reich said he had seen orgone when he injected his mice with bions and in the sky at night through an "organoscope", a special ... where he conducted experiments on mice with cancer, injecting them with bions. He built a small Faraday cage to examine the ...
Naked Gun 33⅓: The Final Insult. Big Hairy Con. 1996. The Mouse. Himself. ... Naked Gun 33⅓: The Final Insult, Fletch Lives, and Ernest Goes to Jail. He has made guest appearances on several television ...
The winner is Proceedings of the Second International Workshop on Nude Mice. See 1978 Governor General's Awards for a complete ... The first winner is Proceedings of the Second International Workshop on Nude Mice. November 15 - Harold Pinter's play Betrayal ...
Rule, Doug (September 11, 2002). "Getting "Naked" with Amber". Metro Weekly. Retrieved April 15, 2020. The first hit from Naked ... "Into the West" by Annie Lennox is about The Lord of the Rings "So Said Kay" by The Field Mice is based on the film Desert ... "The Field Mice \ Biography". LTM Recordings. Retrieved March 1, 2001. So Said Kay was written about the movie Desert Hearts, ...
"Mebendazole inhibits growth of human adrenocortical carcinoma cell lines implanted in nude mice". Cancer Chemotherapy and ...
"Establishment of a human medulloblastoma cell line and its heterotransplantation into nude mice". J Neuropathol Exp Neurol. 44 ...
It will also form tumors in nude mice in around 3-4 weeks. The loss of neuronal characteristics has been described with ... 4-tetrahydroisoquinoline Derivatives in C57BL Mouse in Vivo". Biological & Pharmaceutical Bulletin. 29 (7): 1401-1403. doi: ...
Zhang ZJ (Feb 2014). "Bufalin attenuates the stage and metastatic potential of hepatocellular carcinoma in nude mice". J Transl ... Venom in mice". Exp Toxicol Pathol. 64 (5): 417-23. doi:10.1016/j.etp.2010.10.007. PMID 21084181.. ...
"Facebook Worm - "Likejacking"". Naked Security. 31 May 2010. Retrieved 23 October 2018. Lekies, Sebastian (2012). "On the ... "Wireless Mouse Hacks & Network Security Protection". MOUSEJACK. Retrieved 3 January 2020. Valotta, Rosario (2011). " ... "CERT VU#981271 Multiple wireless keyboard/mouse devices use an unsafe proprietary wireless protocol". www.kb.cert.org. ... "Viral clickjacking 'Like' worm hits Facebook users". Naked Security. 31 May 2010. Retrieved 23 October 2018. " ...
Extract in Nude Mice Xenografts". Biomed Res Int. 2015: 1-9. doi:10.1155/2015/601015. PMC 4450285. PMID 26090427.CS1 maint: ... Its extract has been found to inhibit hepatocellular carcinoma in vivo in mice and in vitro in human cells.[citation needed] ...
... on glioblastoma growth in nude mice". Neurosurgery. 59 (6): 1304-12, discussion 1312. doi:10.1227/01.NEU.0000245622.70344.BE. ... Preclinical studies in mice of cilengitide were able to demonstrate efficacious tumor regression. In a rat xenograft model, ...
Rae-Venter, B.; Reid, L. M. (1980). "Growth of human breast carcinomas in nude mice and subsequent establishment in tissue ...
In a HepG2 orthotropic HCC xenograft model in nude mice, bafilomycin prevented tumor growth. V-ATPase dysregulation is thought ... In vivo bafilomycin reduced average tumor volume in MCF-7 and MDA-MB-231 xenograft mouse models by 50% and did not show toxic ... Additionally, when combined with sorafenib, bafilomycin also caused tumor regression in MDA-MB-231 xenograft mice. ...
Penelope - A mouse kept by Kentauros. She eventually escapes her cage and joins Tamala in her journey to Orion. Human Mother - ... She is always depicted nude, playing video games with a giant snake wrapped around her. She is determined to not let Tamala ... he has a fetish for sailor boys and keeps a mouse, Penelope, locked in a cage for his pleasure. He is eventually killed in a ... accompanied now by the mouse Penelope, a former sex slave of Kentauros. As she leaves we see through product branding, ...
Expression of T-cadherin leads to complete inhibition of subcutaneous tumor growth in nude mice. Seeding T-cadherin expressing ... "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. University of Tokyo ... 2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci ...
Fogh J, Fogh JM, Orfeo T (1977). "One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice". ...
"Efficacy of sulforaphane in eradicating Helicobacter pylori in human gastric xenografts implanted in nude mice". Antimicrobial ... An H. pylori recN mutant displays an attenuated ability to colonize mouse stomachs, highlighting the importance of ... and are unable to establish successful infection in a mouse model. Similarly, RecN protein plays an important role in DSB ...
Nude mouse Naked mole-rat Guinea pig breed Skinny pig Majchrowicz, Margaret. "Baldwin Origin". Margaret's Hairless Pigs. ...
... true mice and rats, gerbils, spiny mice, crested rat Family Nesomyidae: climbing mice, rock mice, white-tailed rat, Malagasy ... The naked mole rat lives completely underground and can form colonies of up to 80 individuals. Only one female and up to three ... The grasshopper mouse from dry regions of North America feeds on insects, scorpions, and other small mice, and only a small ... Because laboratory mice (house mice) and rats (brown rats) are widely used as scientific models to further our understanding of ...
In mice, the nude mouse strain are congenitally thymic deficient. These mice are an important model of primary T cell ... A fully functional thymus derived from reprogrammed mouse embryonic fibroblasts has been grown in the kidney capsule of mice. ... and upon transplantation it was able to restore immune function in immunocompromised mice. The mouse embryonic fibroblasts were ... Some animals have multiple secondary (smaller) thymi in the neck; this phenomenon has been reported for mice and also occurs in ...
J. Sprent: Migration and Lifespan of Circulating B-Lymphocytes of Nude (nu/nu) Mice. In: Proc. First Intern. Workshop Nude Mice ... s Lymphoma serially grown in nude mice. In: Proceedings of the first international workshop on nude mice. Verlag Gustav Fischer ... B. J. Braakhuis u. a.: The potential of the nude mouse xenograft model for the study of head and neck cancer. In: Archives of ... S. Loisel u. a.: Establishment of a novel human B-CLL-like xenograft model in nude mouse. In: Leukemia Research 29/2005, S. ...
... are not visible to the naked eye when inspecting the skin and require a microscope to be seen.[28] Many features may indicate ... A vaccine against inflammatory acne has shown promising results in mice and humans.[50][196] Some have voiced concerns about ...
"Three Blind Mice" is a medieval English nursery rhyme about three blind mice whose tails are cut off after chasing the farmer's ... while another holds that he was blinded as punishment after he saw Athena naked while she was bathing. In the Odyssey, the one- ... The work is explicitly incongruous, ending with the comment Did you ever see such a sight in your life, As three blind mice? ...
"Genomic maps and comparative analysis of histone modifications in human and mouse". Cell. 120 (2): 169-81. doi:10.1016/j.cell. ... who believed that transcription was activated by protein-DNA and protein-protein interactions on largely naked DNA templates, ... After 7 days of nicotine treatment of mice, acetylation of both histone H3 and histone H4 was increased at the FosB promoter in ... "Genomic instability in mice lacking histone H2AX". Science. 296 (5569): 922-7. Bibcode:2002Sci...296..922C. doi:10.1126/ ...
Finch, Christopher (May 27, 1988). "Chapter 9: The End of an Era". The Art of Walt Disney: From Mickey Mouse to the Magic ... Davies, Jonathan (January 11, 1999). "Dis Calls in 'Rescuers' After Nude Images Found". The Hollywood Reporter.. ... Two of these mice, jittery janitor Bernard (Bob Newhart) and his co-agent, the elegant Miss Bianca (Eva Gabor), set out to ... The mice travel to Medusa's pawn shop, where they discover that she and her partner, Mr. Snoops, are on a quest to find the ...
Most pests concerned at this time are ants, spiders, crickets, cockroaches, ladybugs, yellowjackets, hornets, mice, and rats. ... attracted to the scent of traces of blood unseen by the naked eye.[2] It became apparent to all that the owner of that sickle ...
... famous for describing human behaviour from a zoological perspective in his books The Naked Ape and The Human Zoo.[240][241] ... and the technique behind gene targeting and knockout mice.[325] ...
As of 2018, there are about 20 TARDBP mouse models, a dozen FUS mouse models, and a number of C9orf72, PFN1, and UBQLN2 mouse ... which are features of the disease that can be seen with the naked eye, include skeletal muscle atrophy, motor cortex atrophy, ... the SOD1G93A model remains both the most widely used SOD1 mouse model[136] and the most widely used ALS mouse model overall.[23 ... especially SOD1G93A mice.[23] However, many drug targets that were shown to be effective in the SOD1G93A transgenic mouse ...
They have a naked, wrinkled head and feathered body. The North American wild turkey - Meleagris gallopavo - has five distinct ... Domestic hens (Gallus domesticus) share this opportunistic behaviour and will eat insects, mice, worms, and amphibians. ... mice, lizards, and amphibians, wading in water to hunt for the latter. ...
The largest insect bodies are about as big as a mouse.[13] ... but they can usually be seen with the naked eye. ...
The Country Mouse and the City Mouse Adventures (1999-2004). *Gamerz (1998-2000) ... Almost Naked Animals (2011-13). *Mr. Young (2011-13). *Splatalot! (2011-13) ...
For example, they use their wings to cover the naked skin of the upper legs and flanks to conserve heat, or leave these areas ... The initial reason was probably to prevent mice and rats from climbing down the chain to eat the oil.[92] Another, symbolical ...
Naked Eyes, Devo, and the early work of Tears for Fears and Talk Talk. Giorgio Moroder, Brian Eno, Phil Collins, Howard Jones, ...
... delivered as naked DNA or recombinant protein. Journal of Immunology. 2004, roč. 172, čís. 12, s. 7618-28. PMID 15187142.. ... A pantothenate auxotroph of Mycobacterium tuberculosis is highly attenuated and protects mice against tuberculosis. Nature ...
Update : Baird's Pocket Gopher • Beaver • Black squirrel • Chinchilla • Cypriot Mouse • Eurasian Beaver • House mouse • Idaho ... Rowett nude rat, BALB/c. When in doubt, open a discussion on the article's talk page. ... Update : Baird's Pocket Gopher • Beaver • Black squirrel • Chinchilla • Cypriot Mouse • Eurasian Beaver • House mouse • Idaho ... SMcCandlish ☺ ☏ ¢ ≽ʌⱷ҅ᴥⱷʌ≼ (landraces & formal breeds/strains; coloration genetics; fancy mouse/fancy rat activities; and ...
mexicanus group: Mexican deer mouse (P. mexicanus). *Naked-eared deer mouse (P. gymnotis) ... Cotton mouse - P. gossypinus *Key Largo cotton mouse P. g. allapaticola. *†Chadwick Beach cotton mouse P. g. restrictus - ... Peromyscus is a genus of rodents whose members are commonly referred to as deer mice. They are New World mice only distantly ... Not to be confused with mouse-deer.. "Deer mouse" redirects here. For a broader category of rodents which includes other deer ...
Helpless chicks are termed altricial, and tend to be born small, blind, immobile and naked; chicks that are mobile and ... some birds whose sexes appear similar to the naked eye are distinguished by the presence of ultraviolet reflective patches on ...
Leong, T. M.; Teo, S. C.; Lim, K. K. P. (2009). "The Naked Bulldog Bat, Cheiromeles torquatus in Singapore - past and present ... Their bodies are about the same size as mice. They're insectivores, which means they eat only insects." - K.O.. ... mouse lemur, and others.[83][84][85] Bats can estimate the elevation of their target using the interference patterns from the ... the lesser mouse-eared bat (Myotis blythii) the greater horseshoe bat (Rhinolophus ferrumequinum), and the Indian flying fox ( ...
Kits are born blind and naked, with pale rose skin and a membrane over the ears. At birth, they measure 6.5-7.0 cm in length ... Unlike the European polecat, which feeds mostly on mouse-like rodents, the steppe polecat preys on larger, steppe-dwelling ... a single steppe polecat can destroy at least 200 ground squirrels a year or 1,500 mouse-like rodents in winter alone. It is ...
"Sustained expression of naked plasmid DNA encoding hepatocyte growth factor in mice promotes liver and overall body growth". ... "Mouse PubMed Reference:".. *^ Gallagher, J.T., Lyon, M. (2000). "Molecular structure of Heparan Sulfate and interactions with ... "Enhanced cardioprotective effects by coexpression of two isoforms of hepatocyte growth factor from naked plasmid DNA in a rat ...
Volodarsky and his girlfriend were arrested for stripping naked and simulating public sex. As a result, Volodarsky served six ... had objected to exhibits which depicted Mickey Mouse and Lenin as Jesus Christ. ...
Chehl S, Job C, Hastings R (1985). "Transmission of leprosy in nude mice". Am J Trop Med Hyg. 34 (6): 1161-6. PMID 3914846.. ... Shepard C (1960). "Acid-fast bacilli in nasal excretions in leprosy, and results of inoculation of mice". Am J Hyg. 71: 147-57 ... Rees R, McDougall A (1977). "Airborne infection with Mycobacterium leprae in mice". J Med Microbiol. 10 (1): 63-8. doi:10.1099/ ...
"Flaxseed and its components reduce metastasis after surgical excision of solid human breast tumor in nude mice". Cancer Lett. ...
Fezco breaks into the house of Mouse's supplier and robs him in order to pay Mouse. At the winter formal, Kat seeks out Ethan ... Later, Rue helps Jules take nude photos of herself after Nate sends her a "dick pic". Rue steals pills from Jules' kitchen. A ... Nate threatens to report the nudes Jules has sent him as child pornography unless she keeps quiet about him and his father. ...
The Pinky and the Brain episode "Mice Don't Dance" (3-11a) takes place in the 1939 World's Fair. ... artist Salvador Dalí which contained within it a number of unusual sculptures and statues as well as live nearly-nude ... a Mickey Mouse watch, a Gillette safety razor, a kewpie doll, a dollar in change, a pack of Camel cigarettes, millions of pages ...
... was shown in 2014 to be viable cell line for tumor xenografts in C57BL/6 nude mice,[35] and was subsequently used to ...
Other examples of artificially selected hairless animals include the hairless guinea-pig, nude mouse, and the hairless rat. ... Mammals with reduced amounts of fur are often called "naked", as with the naked mole-rat, or "hairless", as with hairless dogs ... The naked mole-rat has evolved hairlessness, perhaps as an adaptation to their subterranean life-style. Two of the largest ... These are often called "naked" or "hairless". Natural selectionEdit. Some mammals naturally have reduced amounts of fur. Some ...
Naked siRNA Pachyonychia congenita I Completed Pachyonychia Congenita Project NCT00716014 AGN211745 VEGFR1 Naked siRNA Age- ... Mouse oocytes and cells from early mouse embryos lack this reaction to exogenous dsRNA and are therefore a common model system ... Naked siRNA Respiratory syncytial virus infections II Completed Alnylam Pharmaceuticals NCT00658086 PRO-040201 ApoB LNP ... Naked siRNA Ocular hypertension, open-angle glaucoma II Recruiting Sylentis NCT01739244 CEQ508 CTNNB1 Escherichia coli-carrying ...
The forearm is furred on the half closer to the body, but naked on the half closer to the wrist and fingers.[12] ... Taxidermied spectral bat "consuming" a mouse. The spectral bat uses echolocation to navigate, creating short pulses of ...
... observed that these mice also lacked a thymus that they began to be used widely in different... ... Nude was described as another hairless mutant by Flanagan in 1966, but it was not until Pantelouris (1968) ... Nude Mouse Spleen Cell Athymic Nude Mouse Athymic Mouse Secondary Response These keywords were added by machine and not by the ... NZB and BALB/c mice on the immune response of nude mice, in: Proceedings of the 1st International Workshop on Nude Mice (J. ...
Most strains of nude mice are slightly "leaky" and do have a few T cells, especially as they age. For this reason, nude mice ... The phenotype (main outward appearance) of the mouse is a lack of body hair, which gives it the "nude" nickname. The nude mouse ... The genetic basis of the nude mouse mutation is a disruption of the FOXN1 gene. The nomenclature for the nude mouse has changed ... A nude mouse is a laboratory mouse from a strain with a genetic mutation that causes a deteriorated or absent thymus, resulting ...
Charles Rivers portfolio includes inbred and outbred nude mice. They are particularly useful for tumor studies, due to their ... Nude Mice. Nude mice were the first immunocompromised mouse strain to be used in cancer research. Our nude mouse models are ... What are nude mice? Nude mouse models contain a genetic mutation that causes the thymus to be absent or deteriorated. This ... Nude Mouse Models. Caused by a genetic mutation, nude mouse models lack a normal immune system and thymus gland. They have a ...
Paralysis in nude mice caused by polyomavirus-induced vertebral tumors.. Harper JS 3rd, Dawe CJ, Trapp BD, McKeever PE, Collins ... Eighty-one of 95 BaLB/c nude mice infected intracranially with the LID strain of polyomavirus developed tumors of the vertebrae ... Wallerian degeneration was the only pathological alteration found in the nervous system of polyoma-infected mice. While ...
... mouse is a useful model for studying the biology and response to therapies of human tumors in vivo. A survey of recent ... Balkwill FR, Proietti E (1986) Effects of mouse interferon on human tumour xenografts in the nude mouse host. Int J Cancer 38: ... The athymic (nude) mouse is a useful model for studying the biology and response to therapies of human tumors in vivo. A survey ... Tumor Size Tumor Volume Nude Mouse Human Tumor Accurate Estimation These keywords were added by machine and not by the authors ...
The naked-eared deer mouse (Peromyscus gymnotis) is a species of rodent in the family Cricetidae. It is found in El Salvador, ...
... into Nude mouse submitted to liver injury. Results are discussed and compared to other available immunodeficient mouse models. ... The Nude Mouse as Model for Liver Deficiency Study and Treatment and Xenotransplantation. Isabelle Vidal1,2 and Lysiane Richert ... We aimed at reviewing the various uses of Nude mouse for the development of liver deficiency models and evaluation of efficacy ... Altogether, these results show that Nude mouse appears to be a suitable small animal model to expand our insight into liver ...
Transgenic Nude Mouse. uPA-Nude mice were used by Rhim et al. [19] by crossing Alb-uPA transgenic mice with athymic nu/nu mice ... Acute Toxic Liver Injury in Nude Mouse. 2.2.1. Thioacetamide (TA). Various doses were tested on Nude mice, with a 20%-mortality ... Fah−/− (Fumarylacetoacetate Hydrolase) Transgenic Nude Mouse. The transgenic mouse model Fah−/− is a mutant mouse deficient in ... Induction of Liver Cirrhosis in Nude Mouse. We succeeded in inducing cirrhosis in Nude mice, by repeated thioacetamide ...
Nude mouse: a new experimental model for Pneumocystis carinii infection Message Subject. (Your Name) has forwarded a page to ... Experimental work with P. carinii in the nude mouse should be performed in isolators because of the communicability of the ... Experimental infection with both human- and rat-derived Pneumocystis carinii has been produced in nude mice by intrapulmonary ... Most infected mice did not appear ill, and their lungs exhibited a mild and nonspecific inflammatory response. Thus, P. carinii ...
Bark scorpion stings can kill other mammals of similar size, yet it seems to act as a painkiller in grasshopper mice, rather ... Researchers led by Ashlee Rowe in the US have reported that little grasshopper mice are resistant to the painful effects of the ... The Naked Scientists® 2000-2020 , The Naked Scientists® and Naked Science® are registered trademarks created by Dr Chris Smith ... Bark scorpion stings can kill other mammals of similar size, yet it seems to act as a painkiller in grasshopper mice, rather ...
Mice, like us, can normally only see whats called the visible light range. But this has changed with the use of cleverly ... The Naked Scientists® 2000-2018 , The Naked Scientists® and Naked Science® are registered trademarks created by Dr Chris Smith ... And as a final test they actually did tests where they show the mice different patterns and they tried to teach the mouse to do ... Theyve injected these nanoparticles into the mices eyes and they have shown that mice can indeed see the infrared light. ...
... their tumorigenesis occurred in 10 nude mice without exception after a subcutaneous injection and did the same in 20 nude mice ... Stable fluorescent protein-expressing HCCLM3-R and HCCLM3-G xenografts in nude mice could be of two useful models for studying ... Biological characteristics of fluorescent protein-expressing human hepatocellular carcinoma xenograft model in nude mice.. Yang ... were subcutaneously injected and two patient-like metastatic models of HCCLM3-R and HCCLM3-G in nude mice were established ...
... and microbacteria composition change by establishing androgen-independent PCa cell PC-3 xenograft nude mice. Nude mice were ... Astaxanthin Inhibits PC-3 Xenograft Prostate Tumor Growth in Nude Mice Mar Drugs. 2017 Mar 8;15(3):66. doi: 10.3390/md15030066 ... Mice were intragastrically administered 100 mg/kg astaxanthin (HA), 25 mg/kg astaxanthin (LA), or olive oil (TC). The results ... and Lachnospiraceae in mice stools, while there was no significant difference between LA and TC groups. These results provide a ...
... and microbacteria composition change by establishing androgen-independent PCa cell PC-3 xenograft nude mice. Nude mice were ... and Lachnospiraceae in mice stools, while there was no significant difference between LA and TC groups. These results provide a ... Mice were intragastrically administered 100 mg/kg astaxanthin (HA), 25 mg/kg astaxanthin (LA), or olive oil (TC). The results ... and microbacteria composition change by establishing androgen-independent PCa cell PC-3 xenograft nude mice. Nude mice were ...
Histologically-intact patient breast tumor tissue was transplanted as intact tissue to the mammary fat pad of nude mice where ... We report here the development of an orthotopic-transplant model of human patient breast cancer in nude mice. ... A Metastatic Orthotopic-Transplant Nude-Mouse Model of Human Patient Breast Cancer Anticancer Res. Jul-Aug 1993;13(4):901-4. ... Histologically-intact patient breast tumor tissue was transplanted as intact tissue to the mammary fat pad of nude mice where ...
What is Athymic nude mouse? Meaning of Athymic nude mouse medical term. What does Athymic nude mouse mean? ... Looking for online definition of Athymic nude mouse in the Medical Dictionary? Athymic nude mouse explanation free. ... nude mouse. (redirected from Athymic nude mouse). Also found in: Dictionary, Thesaurus. mouse. [mows] a small rodent, various ... nude mouse a mouse homozygous for the nu gene; these mice are hairless, lack a thymus, and thus lack T lymphocytes. ...
... Areberg, Johan LU ; Wennerberg, Johan LU ; Johnsson, Anders LU ... METHODS AND MATERIALS: Tumor-bearing (human squamous cell carcinoma, AB) nude mice were divided into four groups and given, i.p ... METHODS AND MATERIALS: Tumor-bearing (human squamous cell carcinoma, AB) nude mice were divided into four groups and given, i.p ... METHODS AND MATERIALS: Tumor-bearing (human squamous cell carcinoma, AB) nude mice were divided into four groups and given, i.p ...
Athymic nude mice, which are used in models of human breast cancer metastasis, were fed a stearic acid, linoleic acid ( ... Dietary stearic acid leads to a reduction of visceral adipose tissue in athymic nude mice.. [Ming-Che Shen, Xiangmin Zhao, Gene ... In contrast total body fat was only slightly reduced in the stearic acid diet fed mice when measured by dual-energy x-ray ...
... for nude mice and 79.9 ± 3.7% for WT controls, p , 0.05, Students t test, n = 9 for nude mice and n = 6 for WT mice, Table 2 ... Response to light pulses in nude and WT mice. Athymic nude and control WT mice were subjected to light pulses during the early ... Activity patterns of nude and WT mice in LD and DD conditions. Representative actograms of nude (left) and wild-type (right) ... In the advances phase shift, nude mice took 7.5 ± 0.5 (n = 6) while WT mice took 5.7 ± 0.2 (n = 3) days to reentrain to the new ...
Title: Enhancement of bone marrow allografts from nude mice into mismatched recipients by T cells void of graft-versus-host ... Journal Article: Enhancement of bone marrow allografts from nude mice into mismatched recipients by T cells void of graft- ... MAT.; BONE MARROW CELLS; TRANSPLANTS; LYMPHOCYTES; BIOLOGICAL FUNCTIONS; GRAFT-HOST REACTION; LECTINS; MICE; RADIATION CHIMERAS ... To ensure engraftment, a large inoculum of nude bone marrow must be supplemented with a trace number of donor T cells, whereas ...
... a mouse with an inherited problem that prevents all of them from developing locks also stops them from immunologically ... Definition for "nude mouse"*a mouse with an inherited problem that prevents… ... Medical Dictionary for "nude mouse"*A mutant mouse said to be… ... How would you define nude mouse?. All the definitions on AZdictionary were written by people just like you. Nows your chance ...
... remained at a higher level in the lungs of nude mice receiving IL-1 than in control mice. In addition, IL-1 injected 1 h, but ... Interleukin 1-induced Augmentation of Experimental Metastases from a Human Melanoma in Nude Mice. Raffaella Giavazzi, Angela ... Interleukin 1-induced Augmentation of Experimental Metastases from a Human Melanoma in Nude Mice ... Interleukin 1-induced Augmentation of Experimental Metastases from a Human Melanoma in Nude Mice ...
... and xenografted into nude mice. The drugs tested were: 4′-epidoxorubicin; 4′-deoxydoxorubicin; and 4′-O-methyldoxorubicin. Mice ... New Doxorubicin Analogs Active against Doxorubicin-resistant Colon Tumor Xenografts in the Nude Mouse. Fernando C. Giuliani and ... New Doxorubicin Analogs Active against Doxorubicin-resistant Colon Tumor Xenografts in the Nude Mouse ... New Doxorubicin Analogs Active against Doxorubicin-resistant Colon Tumor Xenografts in the Nude Mouse ...
Radioimmunoscintigraphy with a novel monoclonal antiprostate antibody (E4): an experimental study in nude mice.. Rydh, Anders ...
Gastric cancer xenografts were established in BALB/C nude mice, and the mice were treated with phosphate-buffered saline (PBS ... This study investigated MSC-delivered NK4 gene therapy in nude mice bearing gastric cancer xenografts. MSCs were transduced ... Tumor growth, tumor cell apoptosis and intratumoral microvessel density of tumor tissue were measured in nude mice bearing ... and tumor cellular apoptosis was significantly induced in the mice treated with MSCs-NK4 compared to control mice. These ...
In athymic nude mice, infection with schistosomes of the same duration and intensity (in terms of the number of eggs in the ... enzymes were investigated in congenitally athymic homozygotic nude mice and in a heterozygotic strain of BALB/c derived mice. ... Therefore, the severe reductions of the hepatic drug-metabolizing function in this infection occur only in mice that are ... liver) as in heterozygotic mice resulted in a much smaller reduction in hepatic drug-metabolizing enzyme activities. ...
Pillars Article: New Member of the Winged-Helix Protein Family Disrupted In Mouse And Rat Nude Mutations. Nature. 1994. 372: ... Pillars Article: New Member of the Winged-Helix Protein Family Disrupted In Mouse And Rat Nude Mutations. Nature. 1994. 372: ... Pillars Article: New Member of the Winged-Helix Protein Family Disrupted In Mouse And Rat Nude Mutations. Nature. 1994. 372: ... Pillars Article: New Member of the Winged-Helix Protein Family Disrupted In Mouse And Rat Nude Mutations. Nature. 1994. 372: ...
... into nude mice. When 1 × 107 SNU-398 cells were injected s.c., the nude mice bore tumors of ∼300 mm3 on day 8. The mice were ... and IL-2 into xenografted nude mice. Before engrafting HCC into nude mice, we tested the expression of intracellular HBx ... Cell lines and mice. C57BL/6-based nude mice were purchased from Charles River Laboratories (Wilmington, MA). T2 cells were ... After the LC-46 clone was injected into tumor-bearing nude mice, 200 μl blood was isolated via tail vein from each mouse, ...
The novel HDAC-inhibitor Panobinostat inhibits growth of human hepatocellular carcinoma xenografts in nude mice. Pietro Di ... The novel HDAC-inhibitor Panobinostat inhibits growth of human hepatocellular carcinoma xenografts in nude mice ... The novel HDAC-inhibitor Panobinostat inhibits growth of human hepatocellular carcinoma xenografts in nude mice ... The novel HDAC-inhibitor Panobinostat inhibits growth of human hepatocellular carcinoma xenografts in nude mice ...
1987) A Nude-Mouse-Grown NPC Tumor Produces Interleukin-1. In: Levine P.H., Ablashi D.V., Nonoyama M., Pearson G.R., Glaser R ...
  • Early work using nude mice for studying T-cell differentiation and the role of T cells in antibody- and cell-mediated responses was soon overwhelmed by oncologic studies when it was established that many human tumors could be grown in nude mice and that the tumors showed only minor changes in their growth patterns and morphology. (springer.com)
  • Nude mice are ideal for tumor and tissue studies because they have no rejection responses and are hairless, making it easier to identify tumors. (criver.com)
  • Paralysis in nude mice caused by polyomavirus-induced vertebral tumors. (nih.gov)
  • Eighty-one of 95 BaLB/c nude mice infected intracranially with the LID strain of polyomavirus developed tumors of the vertebrae which compressed the spinal cord or peripheral nerves and produced paralysis. (nih.gov)
  • The athymic (nude) mouse is a useful model for studying the biology and response to therapies of human tumors in vivo. (springer.com)
  • A survey of recent literature revealed the use of 19 different formulas for determining the size of subcutaneous tumors grown as xenografts in nude mice (2 for determining tumor area, 3 for tumor diameter, and 14 for calculating tumor volume). (springer.com)
  • We compared the volumes, areas, and diameters predicted by each of the 19 formulas with the actual weights of 50 tumors ranging from 0.46 to 22.0 g established in nude mice as xenografts from human cell lines. (springer.com)
  • Auerbach R, Morrissey LW, Sidky YA (1978) Regional differences in the incidence and growth of mouse tumors following intradermal or subcutaneous inoculation. (springer.com)
  • Bullard DE, Schold SC, Bigner SH, Bigner DD (1981) Growth and chemotherapeutic response in athymic mice of tumors arising from human glioma-derived cell lines. (springer.com)
  • Given its lack of functional T lymphocytes, due to a failure in thymus development, mice carrying the Foxn1(Δ/Δ) mutation (nude mice) have been traditionally used in studies including implantation of xenogeneic tumors. (jcircadianrhythms.com)
  • The antitumor activity of doxorubicin and three new derivatives modified on the position 4′ of the amino sugar was tested against five human colon tumors and two human rectal tumors (originating from different patients) and xenografted into nude mice. (aacrjournals.org)
  • Mice were treated i.v. on a weekly basis for 3 to 4 weeks, starting when the tumors were well established (advanced stage of tumor treatment). (aacrjournals.org)
  • When LC-46 cells were adoptively transferred into xenografted nude mice bearing human hepatomas expressing HLA-A2.1 molecules and intracellular HBx proteins, the tumors were eradicated. (jimmunol.org)
  • Notably, when an HBx 52-60-specific clone (LC-46) was adoptively transferred into nude mice xenografted with HCC, the tumors were eradicated. (jimmunol.org)
  • The distribution of the conjugate after i.p. or i.v. injection in nude mice bearing human LN-229 glioma xenografts was confirmed by magnetic resonance imaging, with an increase in signal intensity in all the organs and tumors except for healthy brain parenchyma with an intact blood-brain barrier. (aspetjournals.org)
  • Nude mice with established U251 MG flank tumors (0.5 to 1.0 cm in diameter) were randomly assigned to be treated with 100-µl intratumoral injections of Tf-CRM107 (10 µg) or 454A12-rRA (10 µg), equimolar doses of CRM107 (4.3 µg), 454A12 antibody (7.5 µg), or rRA (1.5 µg), or phosphate-buffered saline (PBS) every 2 days for a total of four doses. (thejns.org)
  • Nude mice with established U251 MG flank tumors (0.5 to 1.0 cm in diameter) were treated with 100-µl intratumoral injections of 10, 1.0, or 0.1 µg of Tf-CRM107 or PBS every 2 days for a total of four doses. (thejns.org)
  • In this study, we show that in vivo systemic LeTx treatment of s.c. xenograft melanoma tumors in athymic nude mice yields partial and complete tumor regressions with minor toxicity to mice. (aacrjournals.org)
  • HE staining xenografted tumors in nude mice. (nih.gov)
  • LMB-H226-GL was found to be able to consistently form solid tumors in the peritoneum of mice. (jcancer.org)
  • Hybridoma-derived monoclonal anti-colorectal carcinoma antibodies suppressed the growth of colorectal carcinoma in nude mice as evidenced by a lower incidence of tumors, a longer latency period, and a smaller volume of tumors in antibody-treated than in control animals. (semanticscholar.org)
  • 131 I-hVEGF siRNA/SilenceMag was then subcutaneously injected into nude mice with HCC tumors exposed to an external magnetic field (EMF). (biomedcentral.com)
  • Female nu/nu mice inoculated subcutaneously with SKOV-3 cells, and treated with OGF (10 mg/kg) for 5 weeks commencing at the time tumors became measurable, had tumor volumes and weight that were reduced by up to 50% from animals receiving saline. (scirp.org)
  • When the same toxic pairing was applied to regular Black-6 lab mice as an experimental control, a cluster of tumors popped up within weeks. (slate.com)
  • In addition to its longevity, naked mole-rats have an extraordinary resistance to cancer as tumors have never been observed in these rodents. (pnas.org)
  • Because of the above features, nude mice have served in the laboratory to gain insights into the immune system , leukemia , solid tumors , AIDS and other forms of immune deficiency as well as leprosy . (hitchhikersgui.de)
  • Tumors developed by using tissue from the same genetic background strain of mouse and used for testing and evaluating new drugs and formulations. (invitekinc.com)
  • The second part tackles the outcome of rat hepatocyte as well as human cell transplantation, both mature hepatocyte and hepatic progenitor, into Nude mouse submitted to liver injury. (hindawi.com)
  • Transplantation of 8 x 10(6) C57BL/6-Nu+/Nu+ (nude) bone marrow cells into C3H/HeJ recipients after conditioning with 8 Gy of total body irradiation has resulted in a markedly higher rate of graft rejection or graft failure compared to that found in recipients of normal C57BL/6 or C57BL/6-Bg+/Bg+ (beige) T-cell-depleted bone marrow. (osti.gov)
  • The goal of this protocol is to illustrate tumorigenicity assay by subcutaneous tumor-cell-transplantation in nude mice. (bio-protocol.org)
  • Immunodeficiency mouse models such as nude (nu) strains and the severe combined immunodeficiency (scid) strains most commonly used for human xenograft tumor transplantation. (invitekinc.com)
  • Biological characteristics of fluorescent protein-expressing human hepatocellular carcinoma xenograft model in nude mice. (nih.gov)
  • Stable fluorescent protein-expressing HCCLM3-R and HCCLM3-G xenografts in nude mice could be of two useful models for studying mechanisms of hepatocellular carcinoma growth and metastasis in real time. (nih.gov)
  • The anti-cancer activity of these two pennogenyl saponins was investigated in nude mice bearing human hepatocellular carcinoma (HCC) HepG2 xenografts. (ovid.com)
  • A nude mouse is a laboratory mouse from a strain with a genetic mutation that causes a deteriorated or absent thymus, resulting in an inhibited immune system due to a greatly reduced number of T cells. (wikipedia.org)
  • Because they lack a thymus, nude mice cannot generate mature T lymphocytes. (wikipedia.org)
  • Nude was described as another hairless mutant by Flanagan in 1966, but it was not until Pantelouris (1968) observed that these mice also lacked a thymus that they began to be used widely in different fields of research. (springer.com)
  • Caused by a genetic mutation, nude mouse models lack a normal immune system and thymus gland. (criver.com)
  • Nude mouse models contain a genetic mutation that causes the thymus to be absent or deteriorated. (criver.com)
  • these mice are hairless, lack a thymus , and thus lack T lymphocytes . (thefreedictionary.com)
  • A type of laboratory mouse that is hairless, lacks a normal thymus gland, and has a defective immune system because of a genetic mutation. (semanticscholar.org)
  • These results suggest that prothymocytes present in nude mice are capable of responding to immunologic stimuli by differentiating, in vitro, into cytolytic T lymphocytes and that furthermore, a major function of the thymus may be to effect the maturation of TCGF-producing cells. (rupress.org)
  • It is suggested that increased number of newly-formed lymphocytes, found in lymph nodes and blood of splenectomized mice, are released from the lympho-myeloid organs in compensation for the loss of long-lived, thymus-derived cells. (ku.dk)
  • Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. (childrensmercy.org)
  • 2016). Thus, nude mice lack a proper thymus, T cell maturation, and may show small amounts of sparse, generally abnormal fur or whiskers that are structurally weak and break as they grow (Mecklenburg et al. (jax.org)
  • The effects of infection with Schistosoma mansoni on the activities of several hepatic drug-metabolizing enzymes were investigated in congenitally athymic homozygotic nude mice and in a heterozygotic strain of BALB/c derived mice. (ajtmh.org)
  • Autoradiography and various quantitations on lymphoid tissues have been used to evaluate the kinetics of small lymphocytes in normal (+/nu or +/+) and congenitally athymic nude (nu/nu) NMRI mice 1 month after splenectomy or sham-splenectomy. (ku.dk)
  • In a xenograft tumor model in nude mice, shRNA-knockdown of COPS3 significantly reduced tumor growth. (jcancer.org)
  • Shen, S. Astaxanthin Inhibits PC-3 Xenograft Prostate Tumor Growth in Nude Mice. (mdpi.com)
  • Ni X, Yu H, Wang S, Zhang C, Shen S. Astaxanthin Inhibits PC-3 Xenograft Prostate Tumor Growth in Nude Mice. (mdpi.com)
  • CONCLUSION: (191)Pt-cisplatin is a more effective drug than nonradioactive cisplatin in retarding tumor growth on nude mice without adding systemic toxic effects. (lu.se)
  • Marked enhancement of donor type chimerism was also found when F1 thymocytes were added to nude bone marrow cells, indicating that the enhancement of bone marrow engraftment by T cells is not only mediated by alloreactivity against residual host cells but may rather be generated by growth factors, the release of which may require specific interactions between T cells and stem cells or between T cells and bone marrow stroma cells. (osti.gov)
  • Modulation of cell cycle regulatory protein expression and suppression of tumor growth by mimosine in nude mice. (spandidos-publications.com)
  • Furthermore, results of TUNEL analysis indicated that mimosine may induce apoptosis to suppress tumor growth in nude mice. (spandidos-publications.com)
  • Chang H, Weng C, Yen M, Chuang L and Hung W: Modulation of cell cycle regulatory protein expression and suppression of tumor growth by mimosine in nude mice. (spandidos-publications.com)
  • RESULTS: HT-29-cell human-colon-carcinoma xenograft mice treated with IP6 showed a significant reduction in tumor growth rate, irrespective of the IP6 dose compared to normal diet group. (koreamed.org)
  • Tumor xenografts from IP6-fed mice showed significantly decreased cancer formation and growth, but increased toxicity was noted for high doses of IP6. (koreamed.org)
  • Imaging prostate tumor growth and lymph node metastasis in nude mice therefore becomes possible using this new fluorescent gene reporter. (spie.org)
  • The tumor growth decreased significantly in mice receiving 131I-labeled peptide compared with the controls and the effect of 131I-labeled peptide can be blocked by unlabeled cNGEGQQc. (ovid.com)
  • A pilot study of the in vivo potency of LeTx by Koo and colleagues showed that the i.t. injection of LeTx inhibits tumor growth in a mouse xenograft melanoma model ( 16 ). (aacrjournals.org)
  • Inhibition of growth of colorectal carcinoma in nude mice by monoclonal antibody. (semanticscholar.org)
  • article{Herlyn1980InhibitionOG, title={Inhibition of growth of colorectal carcinoma in nude mice by monoclonal antibody. (semanticscholar.org)
  • We used a modified orthotopic PC-3 nude mouse tumor model of human prostate cancer to study whether alendronate affects prostate tumor growth and metastasis. (biomedcentral.com)
  • In addition, this investigation assessed whether OGF biotherapy, alone or in combination with taxol or cisplatin, inhibits tumor growth in mice with xenografts of ovarian cancer. (scirp.org)
  • Anti-epidermal growth factor receptor antibody C225 inhibits angiogenesis in human transitional cell carcinoma growing orthotopically in nude mice. (semanticscholar.org)
  • Influence of organ environment on the growth, selection, and metastasis of human colon carcinoma cells in nude mice. (semanticscholar.org)
  • Effect of estrogens and antiestrogens on growth of human breast cancer cells in athymic nude mice. (semanticscholar.org)
  • Tumorigenicity of virus-transformed cells in nude mice is correlated specifically with anchorage independent growth in vitro. (semanticscholar.org)
  • Our results are consistent with our hypothesis and this study demonstrated that intraocular administration of zeaxanthin significantly inhibits the growth and invasion of human uveal melanoma in nude mice, suggesting that zeaxanthin may be a promising agent to be explored for the prevention and treatment of uveal melanoma. (arvojournals.org)
  • Furthermore, we show that a combination of activated Ras and SV40 LT fails to induce robust anchorage-independent growth in naked mole-rat cells, while it readily transforms mouse fibroblasts. (pnas.org)
  • Target cells are transplanted to 6-week-old nude mice subcutaneously and the tumor growth is monitored over a period of observation or treatment. (bio-protocol.org)
  • No significant differences in tumour growth and metastatic behaviour were observed when MOC-25 was injected in ovariectomized nude mice or in male nude mice. (elsevier.com)
  • As noted in the datastrain sheet, these NU/J inbred mice have a homogenous mouse genetic background, which allows for highly consistent and reproducible growth of many allogeneic cell lines. (jax.org)
  • WG-391D inhibited tumor growth in nude mice inoculated with SKOV3 cells or a patient-derived xenograft (PDX). (frontiersin.org)
  • Balkwill FR, Proietti E (1986) Effects of mouse interferon on human tumour xenografts in the nude mouse host. (springer.com)
  • Most strains of nude mice are slightly "leaky" and do have a few T cells, especially as they age. (wikipedia.org)
  • These studies suggest that maturational and/or activation states of the B cells in mice bearing the X(id) or nude genes are different from those seen in the parent strains of mice. (elsevier.com)
  • What nude mouse strains exist, and how do they differ? (jax.org)
  • Today, JAX now maintains several nude strains from NIH. (jax.org)
  • Invitek offers in vivo xenograft tumor models in both nude (nu) and the severe immunodeficiency (scid) strains. (invitekinc.com)
  • The pattern of infection differs markedly from the more common MHV wasting syndrome in nude mice caused by non-enteric strains of MHV. (elsevier.com)
  • In conclusion, u-PA mediated matrix degradation in vitro and production of u-PA and PAI-1 by human melanoma cell lines correlated with their ability to form spontaneous lung metastasis in nude mice. (rupress.org)
  • Nude mice were the first immunocompromised mouse strain to be used in cancer research. (criver.com)
  • Our nude mouse models are ideal for cancer studies because they don't reject tumor cells. (criver.com)
  • Athymic nude mice are the most commonly used model in cancer research. (criver.com)
  • Boven E, Nauta MM, Schluper HM, Elferink F, Vijgh WJ van der, Pinedo HM (1985) Secondary screening of platinum compounds in human ovarian cancer xenografts in nude mice. (springer.com)
  • Nude mice were inoculated with androgen-independent prostate cancer PC-3 cells subcutaneously. (nih.gov)
  • We report here the development of an orthotopic-transplant model of human patient breast cancer in nude mice. (nih.gov)
  • Athymic nude mice, which are used in models of human breast cancer metastasis, were fed a stearic acid, linoleic acid (safflower oil), or oleic acid (corn oil) enriched diet or a low fat diet ad libitum. (sigmaaldrich.com)
  • In this study, we examined the anti-cancer effect of mimosine on human H226 lung cancer cells grown in nude mice. (spandidos-publications.com)
  • When administered at a nontoxic dose of 500 μg/kg on days 7, 9, and 11 to nude mice injected i.v. with the two human lung cancer cell lines, SS1(dsFv)-PE38 selectively inhibited experimental lung metastases produced by the mesothelin-producing NCI-H226 cells. (aacrjournals.org)
  • The first antimesothelin monoclonal antibody, K1, was isolated from mice immunized with the ovarian cancer cell line OVCAR3 ( 15 ). (aacrjournals.org)
  • The incorporation of an empirical approach to dosing in mouse models of cancer in preclinical studies may allow for better prediction of clinical efficacy for ZD6474 alone and in combination with other therapeutic modalities based on equivalent drug exposure. (aspetjournals.org)
  • Nude mice bearing lung cancer cell xenografts (H1975 and L78) were used as an in-vivo model for tumor-inhibition efficacy studies with 131I-cNGEGQQc. (ovid.com)
  • Aim: To establish a pancreatic cancer stem cell model using human pancreatic cancer cells in nude mice to provide a platform for pancreatic cancer stem cell research. (koreascience.or.kr)
  • Materials and Methods: To establish pancreatic cancer xenografts using human pancreatic cancer cell line SW1990, nude mice were randomly divided into control and gemcitabine groups. (koreascience.or.kr)
  • Materials and Methods: A 3-mm fragment of HT29 human colon cancer was implanted to the liver of 5 athymic nude mice (nu/nu). (iiarjournals.org)
  • Naked mole rats: Can they help us cure cancer? (slate.com)
  • Unlike mice, which die of cancer by the crateful, not a single one of her animals has ever developed a naturally occurring tumor. (slate.com)
  • This time she infected cells from a naked mole rat with a virus designed to corrupt their nuclei with the cancer-causing genes SV40 TAg and Ras . (slate.com)
  • Every single mouse had cancer, and every single mouse died. (slate.com)
  • The mechanisms responsible for the cancer resistance of naked mole-rats were unknown. (pnas.org)
  • The naked mole rat lives ten times longer than its distant cousins the rat and the mouse, and the hope is that by comparing their genomes, scientists will unlock some of the genetic and biological secrets of aging and cancer , including in humans. (medicalnewstoday.com)
  • Co-author Dr Rochelle Buffenstein, professor of physiology at the Barshop Institute for Longevity and Aging Studies, part of the UT Health Science Center San Antonio, told the media that aging and biomedical research could learn a lot from the naked mole rat 's capacity to resist cancer and maintain protein integrity in the face of oxidative damage. (medicalnewstoday.com)
  • If we understand which genes are different or are expressed differently in naked mole rats -- compared to short-lived mice that clearly have poor defenses against aging and cancer -- we might find clues as to why the naked mole rat is able to extend both health span and longevity, as well as fight cancer, and this information could be directly relevant and translatable to humans. (medicalnewstoday.com)
  • As well as delayed aging, the naked mole rat is resistant to both spontaneous cancer and experimentally induced tumor formation, it can't feel certain types of pain and is able to rewire its brain (high neural plasticity). (medicalnewstoday.com)
  • Using a phage display library made from spleen mRNA of mice immunized with recombinant mesothelin produced in Escherichia coli , an antimesothelin scFv was isolated ( 17 ). (aacrjournals.org)
  • We found that spleen, lymph node, and bone marrow cells, isolated from nude mice, were incapable of producing TCGF yet responded normally to T-cell mitogen sensitization provided stimulation was conducted in the presence of TCGF. (rupress.org)
  • Nude mouse spleen cells were also capable of responding to alloantigen sensitization in mixed lymphocyte cultures (NLMC) conducted in the presence of TCGF. (rupress.org)
  • Surface immunoglobulin on spleen cells from NZB and NZB/W mice and congenic mice bearing the nude or X-linked immune defective (X(id)) gene was examined by flow microfluorometry with regard to both the frequency of positive cells and density expressed on the cell. (elsevier.com)
  • Spleen cells from these mice were also separated by free-flow electrophoresis and analyzed in a similar manner. (elsevier.com)
  • In this study, we compared the anatomy and tissue morphology of NMR and ICR mouse spleens and found that the gross appearance of the NNMR spleen differed from ICR. (oncotarget.com)
  • Free-running periods of athymic mice and their wild type counterpart were 23.86 ± 0.03 and 23.88 ± 0.05 hours, respectively. (jcircadianrhythms.com)
  • It was in the 1960s that the first publication specifically mentioned nude mice, also known as athymic mice. (jax.org)
  • Brunner N, Spang-Thomsen M, Vindelov L, Wolff J, Engelholm SA (1985) Effect of tamoxifen on the receptor-positive T61 and the receptor-negative T60 human breast carcinomas grown in nude mice. (springer.com)
  • The scientists still don't know why the grasshopper mice don't get poisoned by the stings, but they do hope their discovery could lead to ways to better develop painkillers for humans, rather than mice. (thenakedscientists.com)
  • Their relatively small size, and both genetic and physiological similarity to humans, make mice a model organism for human disease. (jax.org)
  • Wear Personal Protective Equipment (PPE) to help protect the mouse from getting infections from humans. (jax.org)
  • However, molecular nature in mice, rats, and humans, loss of transcription factor Foxn1 the keratinization processes is significantly impaired. (intechopen.com)
  • During the anagen phase hair is actively growing with a progression of tissue proliferation, a short resting phase with a massive apoptosis of hair follicle (catagen) and the relatively quiescence of telogen follicle, thus maintaining hairy phenotype in mice, rats, and humans [ 1 , 2 , 3 ]. (intechopen.com)
  • Stable RFP-expressing or GFP-expressing HCCLM3 cells, namely HCCLM3-R and HCCLM3-G, were subcutaneously injected and two patient-like metastatic models of HCCLM3-R and HCCLM3-G in nude mice were established using surgical orthotopic implantation from subcutaneous tumor tissues. (nih.gov)
  • The phenotype (main outward appearance) of the mouse is a lack of body hair, which gives it the "nude" nickname. (wikipedia.org)
  • Mice with a targeted deletion in the FOXN1 ("knockout" mice) also show the "nude" phenotype. (wikipedia.org)
  • To enhance research efforts of Foxn1 target gene regulation and the pharmacological manipulation of the nude phenotype are important open questions for promising research strategies related with Foxn1 biology. (intechopen.com)
  • How do I select the right nude mouse model? (criver.com)
  • Selecting the most appropriate mouse model is an essential step a researcher makes when designing an oncology study. (criver.com)
  • Altogether, these results show that Nude mouse appears to be a suitable small animal model to expand our insight into liver cell engraftment and regeneration. (hindawi.com)
  • Since athymic Foxn1(Δ/Δ) mice presented no differences with the WT controls in the response of the circadian system to the experimental manipulations performed in this work, we conclude that they represent a good model in studies that combine xenograft implants with either alteration of the circadian schedules or chronopharmacological approaches to therapeutics. (jcircadianrhythms.com)
  • We have developed a new antimetastatic chemotherapeutic strategy for combination with hepatic resection of human colon cancers in a high-metastasis nude mouse model. (aacrjournals.org)
  • The present experimental evidence further supports conclusions gained from previous assays with normal and TTD variant scalp grafts, i.e. that the nude mouse bearing human scalp specimens may serve as a clinically relevant laboratory model for evaluating regulation of normal and abnormal gene expression in the hair follicle under well controlled experimental conditions. (biomedsearch.com)
  • A s.c. xenograft melanoma model in athymic nude mice was treated with different i.p. doses of LeTx. (aacrjournals.org)
  • In this study, we determined the in vivo potency and selectivity of systemically administered LeTx in an athymic nude mouse melanoma model. (aacrjournals.org)
  • The human melanoma cell line we chose to use for the s.c. mouse xenograft model was the SK-MEL-28 cell line. (aacrjournals.org)
  • We further investigated the biological consequence of Id-1 activation in tumorigenesis with the nude mouse xenograft model. (nih.gov)
  • CONCLUSIONS: Both micro-PET/CT and histomorphometric measurement confirmed the bone metastases model of ATC in nude mice by left ventricular cavity injection of ARO cell line. (minervamedica.it)
  • Zhang L, Wang H, Liang S, Ma C. A bone metastases model of anaplastic thyroid carcinoma in athymic nude mice. (minervamedica.it)
  • With increasing efforts devoted to developing therapeutics targeting mesothelioma, a xenograft mouse model with in vivo tumor imaging is especially desired for evaluating anti-tumor therapies. (jcancer.org)
  • The present study aimed to use ICG to visualize small CCLM in an orthotopic mouse model. (iiarjournals.org)
  • Conclusion: Small CCLM can be clearly identified by ICG fluorescence in an orthotopic nude-mouse model. (iiarjournals.org)
  • The present study investigated the in vivo effects of zeaxanthin on human uveal melanoma in a nude mouse model. (arvojournals.org)
  • These characteristics suggest that naked mole-rats have evolved efficient anti-aging defenses, making these rodents an invaluable model organism for aging research. (pnas.org)
  • The mouse is arguably the most widely used mammalian model in biomedical research. (jax.org)
  • Taken together, these characteristics of the nude mice make them an excellent model for xenografting tumor cell lines, understanding mechanisms of tumor malignancy, and testing the impact of potential treatments (Price, 2001). (jax.org)
  • The genetic basis of the nude mouse mutation is a disruption of the FOXN1 gene. (wikipedia.org)
  • The effects of the nude (nu/nu) mutation on bleomycin-induced pulmonary fibrosis. (biomedsearch.com)
  • In the present study, we have evaluated the impact of the athymic (nude) mutation on the development of pulmonary fibrosis in mice induced by a single intratracheal instillation of bleomycin (0.75 units/animal). (biomedsearch.com)
  • The data indicate that the nude athymic mutation protects, at least partially, against bleomycin-induced pulmonary fibrosis, thus suggesting a role for the cellular immune system in regulating the fibrogenic response to this drug. (biomedsearch.com)
  • In 1975, The Jackson Laboratory (JAX) imported the Foxn1nu mutation from the National Institutes of Health (NIH) on an outbred stock of mice. (jax.org)
  • These mice have the Foxn1 nu mutation fully congenic on the C57BL/6J inbred genetic background. (jax.org)
  • Their biomarker expression and karyotype were found to be similar to those of the parental HCCLM3, and their tumorigenesis occurred in 10 nude mice without exception after a subcutaneous injection and did the same in 20 nude mice after an orthotopic implantation. (nih.gov)
  • The in vitro generation and sustained culture of nude mouse cytolytic T-lymphocytes. (rupress.org)
  • Blood lymphocyte numbers were at normal levels in splenectomized nude mice, in spite of reduced numbers of bone marrow and thoracic duct lymphocytes. (ku.dk)
  • These data indicate that although the frequency of unseparated sIg + B lymphocytes is equivalent among all of these groups of mice, the densities of sIgM and sIgD are different. (elsevier.com)
  • abstract = "The cause of emaciation and diarrhea in athymic nude mice was found to be hyperplastic typhlocolitis resulting from infection with enterotropic mouse hepatitis virus (MHV). (elsevier.com)
  • This study has examined the effect of the cytokine interleukin 1 (IL-1) on metastasis formation by the human melanoma A375M in nude mice. (aacrjournals.org)
  • Metastatic behavior of human melanoma cell lines in nude mice correlates with urokinase-type plasminogen activator, its type-1 inhibitor, and urokinase-mediated matrix degradation. (rupress.org)
  • Cultured human uveal melanoma cells from an immortal cell line were inoculated into the choroid of nude mice. (arvojournals.org)
  • Experimental infection with both human- and rat-derived Pneumocystis carinii has been produced in nude mice by intrapulmonary injection of lung homogenates that contained P. carinii and by environmental transmission. (sciencemag.org)
  • Each mouse was imaged using a dedicated small-animal PET/CT scanner after successful injection of [18F]-FDG under deep anesthesia. (minervamedica.it)
  • When the tumor grew to a volume of $125mm^3$ , they treated with gemcitabine at a dose of 50mg/kg by intraperitoneal injection of 0.2ml in the gemcitabine group, while the mice in control group were treated with the same volume of normal saline. (koreascience.or.kr)
  • Mice were randomly divided into control group (without treatment of zeaxanthin), zeaxanthin low group and zeaxanthin high group (treated with intra-choroidal injection of zeaxanthin at 0.171 mg and 0.684 mg, respectively). (arvojournals.org)
  • Hence, this nude gene lack of function makes very similar hair pattern baldness in both human and nude mice (Foxn1nu/Foxn1nu). (intechopen.com)
  • The Nude Mouse in Experimental and Clinical Research (Vol.1). (wikipedia.org)
  • Giovanella, B.C.(eds) Academic Press, 1978, ISBN 0-12-261860-2 The Nude Mouse in Experimental and Clinical Research (Vol.2). (wikipedia.org)
  • Experimental work with P. carinii in the nude mouse should be performed in isolators because of the communicability of the organism. (sciencemag.org)
  • 0.01 µg per mouse i.v. given before tumor cells) induced an augmentation of experimental lung metastases from the A375M tumor cells in nude mice. (aacrjournals.org)
  • Radioimmunoscintigraphy with a novel monoclonal antiprostate antibody (E4) : an experimental study in nude mice. (diva-portal.org)
  • However, these mice may breed better, live longer, and may be better able to withstand surgical procedures and other experimental manipulation (Zeineldin M et al. (jax.org)
  • Material derived from an experimental mouse was passed into neonatal Swiss mice and caused acute typhlocolitis. (elsevier.com)
  • Berkelhammer J, Luethans TN, Hook RR Jr, Oxenhandler RW (1986) Phenotypic instability of mouse melanomas after propagation in vivo and in vitro. (springer.com)
  • In vitro, both asialo-GM1+ and asialo-GM1- nude mouse splenocytes produced IFN-gamma in response to IL-2. (rupress.org)
  • The observation that thymocytes responded normally to T-cell mitogens in the presence of TCGF, prompted the investigation of the effect of TCGF on nude mouse lymphocyte responses in vitro. (rupress.org)
  • Nude mice have a spontaneous deletion in the FOXN1 gene. (wikipedia.org)
  • Attia MA, Weiss DW (1966) Immunology of spontaneous mammary carcinomas in mice with acquired tumor resistance and enhancement in strain A mice infected with mammary tumor virus. (springer.com)
  • The domestic and foreign scholars have studied naked mole rats more focused on the respect such as its long life, resistant to low oxygen, little spontaneous tumor, but the study of the immune system is little. (oncotarget.com)
  • METHODS: HT-29 cells were injected into nude mice. (koreamed.org)
  • ATC cell line ARO cells were injected into the left ventricular cavity of each mouse respectively. (minervamedica.it)
  • A mutant strain of lab mouse that is hairless, thymusless due to congenital thymic aplasia, and T cell-less. (thefreedictionary.com)
  • inbred strain of laboratory mouse which is hairless and has no thymic tissue. (thefreedictionary.com)
  • These results show that an enterotropic strain of MHV can cause chronic enteric disease in athymic nude mice. (elsevier.com)
  • PURPOSE: To investigate the effect of (191)Pt-cisplatin in vivo in terms of the antitumor effect and general toxicity on tumor-bearing nude mice. (lu.se)
  • METHODS AND MATERIALS: Tumor-bearing (human squamous cell carcinoma, AB) nude mice were divided into four groups and given, i.p., physiological saline (controls), cisplatin, (191)Pt-cisplatin (80 MBq/mg), or (191)Pt-cisplatin (160 MBq/mg), respectively. (lu.se)
  • No differences in mortality between the different groups could be observed and no significant differences in weight change between the mice treated with cisplatin or (191)Pt-cisplatin could be seen. (lu.se)
  • In the present study, PAMAM G4 NH2 and Biotin PAMAM G4 NH2 dendrimer cisplatin complexes were administered intraperitoneally to SKOV xenografted athymic nude mice at a dose of 4 mg/kg. (omicsonline.org)
  • The increase in lifespan of tumor bearing mice and biodistribution of cisplatin in various organs was evaluated after IP administration of cisplatin and dendrimer-cisplatin complexes. (omicsonline.org)
  • The lifespan of mice increased by 41.93% and 25.8%, after treatment with PAMAM G4 NH2 and Biotin PAMAM G4 NH2 dendrimer cisplatin complexes, respectively. (omicsonline.org)
  • The combination of OGF with taxol (3 mg/kg, weekly) or cisplatin (4 mg/kg, weekly for 2 weeks) for 37 days reduced tumor volumes and weight in contrast to mice receiving individual agents alone. (scirp.org)
  • Moreover, OGF treatment in mice receiving cisplatin provided protection against the weight loss associated with cisplatin alone. (scirp.org)
  • Nude mice are housed in isolators that maintain a microbiologically controlled environment. (criver.com)
  • In vivo, HepG2 cells were xenografted to male NMRI mice (n/group = 8) and treated with daily intraperitoneal injections of 10 mg/kg Panobinostat. (aacrjournals.org)
  • Feng M, Zhang J, Anver M, Hassan R, Ho M. In Vivo Imaging of Human Malignant Mesothelioma Grown Orthotopically in the Peritoneal Cavity of Nude Mice. (jcancer.org)
  • The in vivo tumorigenicity of Luc/GFP-labeled LMB-H226-GL was determined by using intraperitoneal injections of the cells in nude mice. (jcancer.org)
  • To evaluate the in vivo efficacy of drugs targeting mesothelioma, we treated mice with SS1P, a recombinant immunotoxin currently evaluated in Phase II clinical trials for treatment of mesothelioma. (jcancer.org)
  • In vivo assay of human NK-dependent ADCC using NOD/SCID/gammac(null) (NOG) mice. (semanticscholar.org)
  • FOXN1 - 600838 http://animalresearch.info/en/designing-research/249/mouse-immunodeficient-/ "Archived copy" (PDF). (wikipedia.org)
  • General activity circadian rhythms in 2-4 month-old Foxn1(Δ/Δ) mice (from Swiss Webster background) and their corresponding wild type (WT) controls was recorded. (jcircadianrhythms.com)
  • For this reason, nude mice are less popular in research today, since knockout mice with more complete defects in the immune system have been constructed (e.g. (wikipedia.org)
  • Busuttil A, O'Conor GT, Foster ME, Gurtsevitch V, Morten JE, Steel CM (1986) The gross pathology and histological features of tumours produced by inoculation of human cell lines into immune-deprived mice. (springer.com)
  • Since the immune system is able to modulate the circadian clock, we investigated if there were alterations in the circadian system of the athymic mutant mice. (jcircadianrhythms.com)
  • Charles River offers multiple immunodeficient mice, such as the athymic nude mouse, for use in basic research and nonclinical drug discovery and development. (criver.com)
  • Results are discussed and compared to other available immunodeficient mouse models. (hindawi.com)
  • Nude mice do require additional precautions as they are immunodeficient. (jax.org)
  • By looking at the gene sequences for the receptor molecules that respond to the toxin, the researchers discovered a single crucial difference between the receptors in the grasshopper mice and those in other mammals that do feel pain from the scorpion's stings. (thenakedscientists.com)
  • knockout mouse a mouse that has had a specific gene artificially deleted from its genome. (thefreedictionary.com)
  • When HBsAg-specific CTLs are adoptively transferred into HBV transgenic mice, HBV gene expression and replication are abolished by the secretion of IFN-γ and TNF-α after Ag recognition ( 17 , 18 ). (jimmunol.org)
  • Then human PCa PC3 cell lines expressing iRFP gene reporter were orthotopically implanted in male Nu/Nu mice at 8-10 weeks old. (spie.org)
  • 2004). In the 50-plus years since the discovery of nude mice, the field has changed significantly due to the development of gene-targeting technology. (jax.org)
  • In contrast to control animals treated with saline, total lung hydroxyproline in the nude mouse was not significantly increased at 14 and 30 days after bleomycin treatment. (biomedsearch.com)
  • However, lung collagen synthetic rates, normalized to dry lung weights, were significantly higher at 14 days in euthymic bleomycin-treated control mice than in the nude bleomycin-treated animals. (biomedsearch.com)
  • peritoneal mouse a free body in the peritoneal cavity, probably a small detached mass or omentum, sometimes visible radiographically. (thefreedictionary.com)
  • METHODS: Eight 6-8 week old female athymic nude mice were randomly selected. (minervamedica.it)
  • Since nude females have underdeveloped mammary glands and are unable to effectively nurse their young, nude males are bred with heterozygous females. (wikipedia.org)
  • Histologically-intact patient breast tumor tissue was transplanted as intact tissue to the mammary fat pad of nude mice where the tumor tissue grew extensively and metastasized to the lung. (nih.gov)
  • 5-[ 125 I]Iodo-2′-deoxyuridine-radiolabeled A375M tumor cells injected i.v. remained at a higher level in the lungs of nude mice receiving IL-1 than in control mice. (aacrjournals.org)
  • Mouse health directly correlates with data integrity and scientific reproducibility. (jax.org)
  • Naked mole-rats are native to Kenya, Ethiopia and Somalia. (cornell.edu)
  • There's a problem with the colonies of naked mole rats that Rochelle Buffenstein carted down to her new lab in Texas four years ago. (slate.com)
  • Earlier this year, one of Buffenstein's graduate students tried smearing the skin of half a dozen naked mole rats with a pair of vicious carcinogens: A synthetic compound called DMBA and an inflammatory agent known as TPA . (slate.com)
  • The naked mole rats went on skittering through their tubes. (slate.com)
  • Naked mole-rats live in large subterranean colonies in Africa. (pnas.org)
  • Naked mole-rats display exceptional longevity, with a maximum lifespan exceeding 28 years ( 2 , 3 ). (pnas.org)
  • It was suggested that naked mole-rats display negligible senescence ( 3 ), characterized by very slow or nonexistent changes in physiological parameters with age, and the lack of an age-related increase in mortality rate ( 6 ). (pnas.org)
  • Accordingly, naked mole-rats do not exhibit age-related changes in basal metabolism, body composition, or bone mineral density ( 3 , 7 ). (pnas.org)
  • Furthermore, naked mole-rats reproduce prolifically until death ( 2 , 3 ). (pnas.org)
  • A large team of international researchers, including members of the University of Texas (UT) Health Science Center at San Antonio in the US, where there is a colony of more than 2,000 naked mole rats, worked on the study, which was published in Nature on 12 October. (medicalnewstoday.com)
  • In the wild, naked mole rats live underground in full darkness in large family groups. (medicalnewstoday.com)
  • We aimed at reviewing the various uses of Nude mouse for the development of liver deficiency models and evaluation of efficacy of hepatic cell xenotransplantation. (hindawi.com)
  • To study biological characteristics of stable red fluorescent protein (RFP)-expressing or green fluorescent protein (GFP)-expressing HCCLM3 cell lines and those of their relevant xenograft models in nude mice. (nih.gov)
  • This study used intragastric administration of astaxanthin to detect its role on tumor proliferation, apoptosis, microRNA (miRNA) overexpression, and microbacteria composition change by establishing androgen-independent PCa cell PC-3 xenograft nude mice. (nih.gov)
  • Mixing experiments using different numbers of nude bone marrow cells with or without mature thymocytes (unagglutinated by peanut agglutinin) revealed that engraftment of allogeneic T-cell-depleted bone marrow is T-cell dependent. (osti.gov)
  • Tumorigenicity and metastasis of human breast carcinoma cell lines in nude mice. (semanticscholar.org)
  • The metastatic capacity of these cell lines was studied in nude mice. (rupress.org)
  • Such alterations in cell-surface antigens correlate with the differences in the natural history of immunopathology of the autoimmune disease in these congenic colonies of New Zealand mice. (elsevier.com)
  • In cell culture, naked mole-rat fibroblasts arrest at a much lower density than those from a mouse. (pnas.org)
  • Invitek intends to establish more cell lines of the NCI panel which are tumorigenic in mice. (invitekinc.com)
  • The disease was reproduced in experimentally-inoculated nude mice using intestinal homogenates from affected mice and cell culture-derived virus. (elsevier.com)
  • Virus failed to grow in NCTC-1469 cells and 17Cl-1 cells, which are normally permissive for MHV, but grew to low titer in a mouse rectal carcinoma cell line, CMT 93. (elsevier.com)
  • Fingerprint Dive into the research topics of 'Enterotropic mouse hepatitis virus infection in nude mice. (elsevier.com)
  • The MOC-25 tumour arose spontaneously in a female nude mouse and was established as a continuous line intraperitoneally in nude mice, where it reproduces the topological features of its origin, growing preferentially in the uterus, ovaries and liver. (elsevier.com)
  • RESULTS: Histopathology established ATC bone metastases in five nude mice's tibia. (minervamedica.it)