A family of gram-negative, aerobic bacteria utilizing only one-carbon organic compounds and isolated from in soil and water.
The simplest saturated hydrocarbon. It is a colorless, flammable gas, slightly soluble in water. It is one of the chief constituents of natural gas and is formed in the decomposition of organic matter. (Grant & Hackh's Chemical Dictionary, 5th ed)
A family of gram-negative methanotrophs in the order Rhizobiales, distantly related to the nitrogen-fixing and phototrophic bacteria.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
A genus of gram-negative, aerobic, straight, curved, or branched rods which are motile by a single polar flagellum. (From Bergey's Manual of Determinative Bacteriology, 9th ed)
A genus of gram-negative rods which form exospores and are obligate methanotrophs.
A genus of gram-negative, aerobic, spherical cells usually occurring in pairs. The resting stage is considered a cyst. (From Bergey's Manual of Determinative Bacteriology, 9th ed)
A family of gram-negative bacteria in the order Methylophilales.
Identification and measurement of ELEMENTS and their location based on the fact that X-RAYS emitted by an element excited by an electron beam have a wavelength characteristic of that element and an intensity related to its concentration. It is performed with an electron microscope fitted with an x-ray spectrometer, in scanning or transmission mode.
A genus of gram-negative, sheathed, rod-shaped bacteria in the family COMAMONADACEAE.
The genomic analysis of assemblages of organisms.
Metals with high specific gravity, typically larger than 5. They have complex spectra, form colored salts and double salts, have a low electrode potential, are mainly amphoteric, yield weak bases and weak acids, and are oxidizing or reducing agents (From Grant & Hackh's Chemical Dictionary, 5th ed)
A species of acetate-oxidizing bacteria, formerly known as Acetobacter xylinum.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A group of the proteobacteria comprised of facultatively anaerobic and fermentative gram-negative bacteria.
Drugs that inhibit the transport of neurotransmitters into axon terminals or into storage vesicles within terminals. For many transmitters, uptake determines the time course of transmitter action so inhibiting uptake prolongs the activity of the transmitter. Blocking uptake may also deplete available transmitter stores. Many clinically important drugs are uptake inhibitors although the indirect reactions of the brain rather than the acute block of uptake itself is often responsible for the therapeutic effects.
Nanometer-scale wires made of materials that conduct electricity. They can be coated with molecules such as antibodies that will bind to proteins and other substances.
A group of PROTEOBACTERIA represented by morphologically diverse, anaerobic sulfidogens. Some members of this group are considered bacterial predators, having bacteriolytic properties.
Habitat of hot water naturally heated by underlying geologic processes. Surface hot springs have been used for BALNEOLOGY. Underwater hot springs are called HYDROTHERMAL VENTS.
A family of bacteria found in the mouth and intestinal and respiratory tracts of man and other animals as well as in the human female urogenital tract. Its organisms are also found in soil and on cereal grains.
Chemical groups containing the covalent sulfur bonds -S-. The sulfur atom can be bound to inorganic or organic moieties.
A flammable, poisonous gas with a characteristic odor of rotten eggs. It is used in the manufacture of chemicals, in metallurgy, and as an analytical reagent. (From Merck Index, 11th ed)
An island in the Lesser Antilles, one of the Windward Islands. Its capital is Fort-de-France. It was discovered by Columbus in 1502 and from its settlement in 1635 by the French it passed into and out of Dutch and British hands. It was made a French overseas department in 1946. One account of the name tells of native women on the shore calling "Madinina" as Columbus approached the island. The meaning was never discovered but was entered on early charts as Martinique, influenced by the name of St. Martin. (From Webster's New Geographical Dictionary, 1988, p734 & Room, Brewer's Dictionary of Names, 1992, p339)
Widely scattered islands in the Atlantic Ocean as far north as the AZORES and as far south as the South Sandwich Islands, with the greatest concentration found in the CARIBBEAN REGION. They include Annobon Island, Ascension, Canary Islands, Falkland Islands, Fernando Po (also called Isla de Bioko and Bioko), Gough Island, Madeira, Sao Tome and Principe, Saint Helena, and Tristan da Cunha.
A condition in which closely related persons, usually in the same family, share the same delusions.
Heat- and storage-labile plasma glycoprotein which accelerates the conversion of prothrombin to thrombin in blood coagulation. Factor V accomplishes this by forming a complex with factor Xa, phospholipid, and calcium (prothrombinase complex). Deficiency of factor V leads to Owren's disease.
A plasma protein that is the inactive precursor of thrombin. It is converted to thrombin by a prothrombin activator complex consisting of factor Xa, factor V, phospholipid, and calcium ions. Deficiency of prothrombin leads to hypoprothrombinemia.
Cylindrical epithelial cells in the innermost layer of the ENAMEL ORGAN. Their functions include contribution to the development of the dentinoenamel junction by the deposition of a layer of the matrix, thus producing the foundation for the prisms (the structural units of the DENTAL ENAMEL), and production of the matrix for the enamel prisms and interprismatic substance. (From Jablonski's Dictionary of Dentistry, 1992)
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
A phylum of bacteria consisting of the purple bacteria and their relatives which form a branch of the eubacterial tree. This group of predominantly gram-negative bacteria is classified based on homology of equivalent nucleotide sequences of 16S ribosomal RNA or by hybridization of ribosomal RNA or DNA with 16S and 23S ribosomal RNA.
A family of nucleocytoplasmic, large, double-stranded DNA viruses with extremely complex genomes.
A person's view of himself.
An island in the Gulf of St. Lawrence constituting a province of Canada in the eastern part of the country. It is very irregular in shape with many deep inlets. Its capital is Charlottetown. Discovered by the French in 1534 and originally named Ile Saint-Jean, it was renamed in 1799 in honor of Prince Edward, fourth son of George III and future father of Queen Victoria. (From Webster's New Geographical Dictionary, 1988, p981 & Room, Brewer's Dictionary of Names, 1992, p433)
Graphic representations, especially of the face, of real persons, usually posed, living or dead. (From Thesaurus for Graphic Materials II, p540, 1995)
1861-1865 conflict between the Union (Northern states) and the 11 Southern states that seceded and were organized as the Confederate States of America.
Individuals' concept of their own bodies.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
Membrane-bound prokaryotic organelles of magnetotactic bacteria that contain chains of MAGNETITE crystals which orient the bacteria to geomagnetic fields.
A genus of microaerophilic, gram-negative bacteria that forms crystals of the mineral magnetite in special organelles called MAGNETOSOMES.
Iron (II,III) oxide (Fe3O4). It is a black ore of IRON that forms opaque crystals and exerts strong magnetism.
Pyrrole containing pigments found in photosynthetic bacteria.
The study of MAGNETIC PHENOMENA.
A phylum of anoxygenic, phototrophic bacteria including the family Chlorobiaceae. They occur in aquatic sediments, sulfur springs, and hot springs and utilize reduced sulfur compounds instead of oxygen.
Ribulose substituted by one or more phosphoric acid moieties.
The discarding or destroying of garbage, sewage, or other waste matter or its transformation into something useful or innocuous.
The relationships of groups of organisms as reflected by their genetic makeup.
A genus of gram-positive, anaerobic bacteria in the family Thermoanaerobacteriaceae. Cultures consist of rods interspersed with coccoid cells.
A family of phototrophic purple sulfur bacteria that deposit globules of elemental sulfur inside their cells. They are found in diverse aquatic environments.
The type species of the genus NITROSOMONAS, a gram-negative chemolithotroph that oxidizes ammonia to nitrite. It is found in soil, sewage, freshwater, and on building walls, and especially in polluted areas where air contains high levels of nitrogen compounds.
A colorless alkaline gas. It is formed in the body during decomposition of organic materials during a large number of metabolically important reactions. Note that the aqueous form of ammonia is referred to as AMMONIUM HYDROXIDE.
DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.

High-affinity methane oxidation by a soil enrichment culture containing a type II methanotroph. (1/236)

Methanotrophic bacteria in an organic soil were enriched on gaseous mixing ratios of <275 parts per million of volume (ppmv) of methane (CH4). After 4 years of growth and periodic dilution (>10(20) times the initial soil inoculum), a mixed culture was obtained which displayed an apparent half-saturation constant [Km(app)] for CH4 of 56 to 186 nM (40 to 132 ppmv). This value was the same as that measured in the soil itself and about 1 order of magnitude lower than reported values for pure cultures of methane oxidizers. However, the Km(app) increased when the culture was transferred to higher mixing ratios of CH4 (1,000 ppmv, or 1%). Denaturing gradient gel electrophoresis of the enrichment grown on <275 ppmv of CH4 revealed a single gene product of pmoA, which codes for a subunit of particulate methane monooxygenase. This suggested that only one methanotroph species was present. This organism was isolated from a sample of the enrichment culture grown on 1% CH4 and phylogenetically positioned based on its 16S rRNA, pmoA, and mxaF gene sequences as a type II strain of the Methylocystis/Methylosinus group. A coculture of this strain with a Variovorax sp., when grown on <275 ppmv of CH4, had a Km(app) (129 to 188 nM) similar to that of the initial enrichment culture. The data suggest that the affinity of methanotrophic bacteria for CH4 varies with growth conditions and that the oxidation of atmospheric CH4 observed in this soil is carried out by type II methanotrophic bacteria which are similar to characterized species.  (+info)

Homologous expression of soluble methane monooxygenase genes in Methylosinus trichosporium OB3b. (2/236)

An homologous expression system has been developed for soluble methane monooxygenase (sMMO) genes from Methylosinus trichosporium OB3b. sMMO-minus mutants were previously obtained after marker-exchange mutagenesis, by the insertion of a kanamycin-resistance cassette into the mmoX gene of the sMMO operon. Complementation of the sMMO-minus genotype was achieved by conjugation with broad-host-range plasmids containing the native promoter and sMMO operon from Ms. trichosporium OB3b (pVK100Sc and pHM2). In wild-type methanotrophs, copper ions present in the growth medium at concentrations greater than 0.25 microM inhibit transcription of sMMO genes. The stable maintenance of pVK100Sc resulted in transconjugant methanotrophs with a decreased sensitivity to copper, since expression of sMMO occurred at copper sulphate concentrations of 7.5 microM. sMMO activity was only detected in soluble extracts after the addition of purified sMMO reductase component, which is inhibited by copper ions in vitro. This phenomenon could have arisen due to the increased number of sMMO gene copies (derived from pVK100Sc) in the cell. Transconjugants obtained from conjugations with pHM2 expressed sMMO at copper concentrations of 0-2.5 microM only and sMMO activity was not restored by the addition of purified reductase component at copper concentrations higher than 2.5 microM. Southern hybridization showed that the plasmid had integrated into the chromosome, probably by a single homologous recombination event. This is the first report of homologous sMMO expression in a methanotroph with enzyme activities that are comparable to the activity reported in wild-type strains. This expression system will be useful for site-directed mutagenesis of active-site residues of sMMO from Ms. trichosporium OB3b.  (+info)

Type 2 Cu2+ in pMMO from Methylomicrobium album BG8. (3/236)

EPR spectra were obtained for the type 2 Cu2+ site in particulate methane monooxygenase (pMMO) from Methylomicrobium album BG8 grown on K15NO3 and 63Cu(NO3)2. The concentration of the type 2 Cu2+ signal was approximately 200 microM per 25 mg/ml protein in packed cells and membrane fractions, a concentration that is consistent with its attribution to pMMO, and the EPR parameters were consistent with electron paramagnetic resonance (EPR) parameters previously assigned to pMMO. The superhyperfine structure due to nitrogen is better resolved because I = 1/2 for 15N whereas I = 1 for 14N and A(15N)/A(14N) = 1.4. Under these conditions, superhyperfine structure is resolved in the g region of the X-band spectrum. At low microwave frequency (S-band) the resolution of the nitrogen superhyperfine structure improves. Signals are attributed to type 2 Cu2+ in which cupric ion is bound to four (less likely three) nitrogen donor atoms.  (+info)

Microbial oxidation of methane and methanol: isolation of methane-utilizing bacteria and characterization of a facultative methane-utilizing isolate. (4/236)

A methane-utilizing organism capable of growth both on methane and on more complex organic substrates as a sole source of carbon and energy, has been isolated and studied in detail. Suspensions of methane-grown cells of this organism oxidized C-1 compounds (methane, methanol, formaldehyde, formate); hydrocarbons (ethane, propane); primary alcohols (ethanol, propanol); primary aldehydes (acetaldehyde, propionaldehyde); alkenes (ethylene, propylene); dimethylether; and organic acids (acetate, malate, succinate, isocitrate). Suspensions of methanol-or succinate-grown cells did not oxidize methane, ethane, propane, ethylene, propylene, or dimethylether, suggesting that the enzymatic systems required for oxidation of these substrates are induced only during growth on methane. Extracts of methane-grown cells contained a particulate reduced nicotinamide adenine dinucleotide-dependent methane monooxygenase activity. Oxidation of methanol, formaldehyde, and primary alcohols was catalyzed by a phenazine methosulfate-linked, ammonium ion-requiring methanol dehydrogenase. Oxidation of primary aldehydes was catalyzed by a phenazine methosulfate-linked, ammonium ion-independent aldehyde dehydrogenase. Formate was oxidized by a nicotinamide adenine dinucleotide-specific formate dehydrogenase. Extracts of methane-grown, but not succinate-grown, cells contained the key enzymes of the serine pathway, hydroxypyruvate reductase and malate lyase, indicating that the enzymes of C-1 assimilation are induced only during growth on C-1 compounds. Glucose-6-phosphate dehydrogenase was induced during growth on glucose. Extracts of methane-grown cells contained low levels of enzymes of the tricarboxylic acid cycle, including alpha-keto glutarate dehydrogenase, relative to the levels found during growth on succinate.  (+info)

Oxidation of ultrafast radical clock substrate probes by the soluble methane monooxygenase from Methylococcus capsulatus (Bath). (5/236)

Radical clock substrate probes were used to assess the viability of a discrete substrate radical species in the mechanism of hydrocarbon oxidation by the soluble methane monooxygenase (sMMO) from Methylococcus capsulatus (Bath). New substituted cyclopropane probes were used with very fast ring-opening rate constants and other desirable attributes, such as the ability to discriminate between radical and cationic intermediates. Oxidation of these substrates by a reconstituted sMMO system resulted in no rearranged products, allowing an upper limit of 150 fs to be placed on the lifetime of a putative radical species. This limit strongly suggests that there is no such substrate radical intermediate. The two enantiomers of trans-1-methyl-2-phenyl-cyclopropane were prepared, and the regioselectivity of their oxidation to the corresponding cyclopropylmethanol and cyclopropylphenol products was determined. The results are consistent with selective orientation of the two enantiomeric substrates in the hydrophobic cavity at the active site of sMMO, specific models for which were examined by molecular modeling.  (+info)

A low-molecular-mass protein from Methylococcus capsulatus (Bath) is responsible for the regulation of formaldehyde dehydrogenase activity in vitro. (6/236)

An 8.6 kDa protein, which the authors call a modifin, has been purified from Methylococcus capsulatus (Bath) and has been shown to alter the substrate specificity and kinetics of NAD+-linked formaldehyde dehydrogenase (FDH) isolated from the same organism. Purification methods for both the modifin and FDH are presented which reliably produced pure protein for further analysis. Analysis of the molecular mass and N-terminal sequence of both FDH and the modifin indicate that they are unique proteins and show no similarity to alcohol or aldehyde dehydrogenase enzymes isolated from methylotrophic bacteria. Substrate specificity studies demonstrated that FDH oxidized formaldehyde exclusively in the presence of the modifin; a diverse range of aldehydes and alcohols were oxidized by FDH in the absence of the modifin. No formaldehyde oxidation was detected in the absence of the modifin. Attempts to replace the modifin with glutathione or high concentrations of methanol to stimulate formaldehyde oxidation failed. With acetaldehyde as substrate, FDH showed standard Michaelis-Menten kinetics; interaction of FDH with the modifin using formaldehyde as substrate altered the kinetics of the reaction to sigmoidal. Kinetic analysis during turnover experiments indicated that the FDH may be associated with bound formaldehyde following enzyme isolation and that NAD may also be associated with the enzyme but in a form that is less tightly bound than found with the methanol dehydrogenase from Bacillus methanolicus. Data are presented which indicate that the modifin may play an important role in regulating formaldehyde concentration in vivo.  (+info)

Microbial oxidation of methane and methanol: crystallization and properties of methanol dehydrogenase from Methylosinus sporium. (7/236)

Obligate methylotrophs are divisible into two types on the basis of ultrastructural biochemical characteristics. Both groups possess a soluble phenazine methosulfate (PMS)-dependent methanol dehydrogenase. In addition, particulate PMS-dependent methanol dehydrogenase and PMS-independent methanol oxidase have been found in the type I membrane group. A procedure was developed for the crystallization of methanol dehydrogenase from the soluble fraction of the type II obligate methylotroph Methylosinus sporium. This is the first report of a crystalline methanol dehydrogenase from a methylotrophic bacterium. The crystallized enzyme is homogeneous as judged by ultracentrifugation and by acrylamide gel electrophoresis. In the presence of an electron acceptor (phenazine or phenazinium compound) and an activator (ammonium compound), the crystallized enzyme catalyzed the oxidation of primary alcohols and formaldehyde. Secondary, tertiary, and aromatic alcohols were not oxidized. The molecular weight of the enzyme as estimated by gel filtration is approximately 60,000, and as estimated by sedimentation equilibrium analysis it is 62,000. The sedimentation constant (S20,W) is 2.9. The subunit size determined by sodium dodecyl sulfate-gel electrophoresis is approximately 60,000. The amino acid composition and spectral properties of the enzyme are also presented. Antisera prepared against the crystalline enzyme are nonspecific, they cross-reacted and inhibited isofunctional enzymes from other obligate methylotrophic bacteria.  (+info)

Some characteristics of bacteria found in a bioreactor to treat trichloroethylene-contaminated groundwater. (8/236)

A mixture of bacteria, having a methane-utilizing ability, was separated from a bioreactor supplied with air and methane gas. The bioreactor was operated to treat trichloroethylene (TCE)-contaminated groundwater. The mixture was composed of an obligate methane-utilizing bacterium and a heterotroph, identified as Methylomonas methanica and Pseudomonas sp., respectively. The mixed culture of these two strains removed TCE. In addition, it appeared that a cooperative metabolic interaction of these strains enabled Meth.methanica to maintain the TCE degradation ability.  (+info)

Methanotrophs are organisms that require methane as a source of carbon and energy for their metabolism. They are gram-negative bacteria that are capable in utilizing methane as a carbon energy source and able to grow both aerobically or anaerobically which only need single-carbon compound to live on. These prokaryotes occur mostly in soils, rice paddles, mud, landfills, and other places where methane is available. They can grow aerobically or anaerobically. In aerobic conditions, they use oxygen and methane to produce formaldehyde. They then incorporate it into organic compounds through ribulose monophosphate pathway or by serine pathway. An example of methanotroph is a gammaproteobacterium that utilize RuMP pathway in assimilating carbon. Such bacteria are referred to as type I methanotrophs. Type II methanotrophs are those from alphaproteobacteria that utilize the serine pathway of carbon assimilation. They have a system of internal membranes where methane oxidation occurs.1 Methanotrophs are ...
A novel moderately thermophilic methanotroph, strain MYHTT, was isolated from a hot spring in Japan. The isolate grew on methane or methanol at 37-67 °C, and optimally at 57-59 °C. It was found to be a Gram-negative aerobe, with colourless colonies of non-motile coccoid cells, possessing type I intracytoplasmic membranes and regularly arranged surface layers of linear (p2) symmetry. Strain MYHTT expressed only the particulate methane monooxygenase and employed the ribulose monophosphate pathway for formaldehyde assimilation. It is a neutrophilic and halotolerant organism capable of growth at pH 6·5-7·5 (optimum pH 6·8) and in up to 3 % NaCl (optimum 0·5-1 % NaCl). Phylogenetic analysis based on 16S rRNA gene sequence analysis indicated that strain MYHTT is most closely related to the thermophilic undescribed methanotroph Methylothermus HB (91 % identity) and the novel halophilic methanotroph Methylohalobius crimeensis 10KiT (90 % identity). Comparative sequence analysis of particulate methane
TY - JOUR. T1 - Different Abilities of Eight Mixed Cultures of Methane-oxidizing Bacteria to Degrade TCE. AU - Broholm, Kim. AU - Christensen, Thomas Højlund. AU - Jensen, Bjørn K.. PY - 1993. Y1 - 1993. N2 - The ability of eight mixed cultures of methane-oxidizing bacteria to degrade trichloroethylene (TCE) was examined in laboratory batch experiments. This is one of the first reported works studying TCE degradation by mixed cultures of methane-oxidizing bacteria at 10°C, a common temperature for soils and groundwaters. Only three of the eight mixed cultures were able to degrade TCE, or to degrade TCE fast enough to result in a significant removal of TCE within the experimental time, when the cultures used methane as growth substrate. The same three mixed cultures were able to degrade TCE when they oxidized methanol, but only for a limited time period of about 5 days. Several explanations for the discontinued degradation of TCE are given. An experiment carried out to re-activate the ...
Methanotrophs (sometimes called methanophiles) are prokaryotes that metabolize methane as their only source of carbon and energy. They can grow aerobically or anaerobically and require single-carbon compounds to survive. These Methanotrophs are currently being modified to absorb methane from the atmosphere, because methane released into the atmosphere contributes to greenhouse gasses, which heat up the earth and cause global warming. Under aerobic conditions, they combine oxygen and methane to form formaldehyde, which is then incorporated into organic compounds via the serine pathway or the ribulose monophosphate (RuMP) pathway. Type I methanotrophs are part of the Gammaproteobacteria and they use the RuMP pathway to assimilate carbon. Type II methanotrophs are part of the Alphaproteobacteria and utilize the Serine pathway of carbon assimilation. They also characteristically have a system of internal membranes within which methane oxidation occurs. Methanotrophs occur mostly in soils, and are ...
Quantification of methanotrophic bacterial biomass.By employing the PLFA concentration per bacterial cell derived by Frostegård and Bååth (15), we estimated the population of methanotrophic bacteria at ca. 7.2 × 106 cells g−1 (dwt) of soil (range, 5.6 × 106 to 8.8 × 106 cells g−1). Incorporation of the 13C label had slowed toward the end of the 11-week incubation, indicating that the microbial biomass had reached, or was close to, a steady state. When considering this methanotrophic biomass estimate, it is important to note that the conversion factor used in the calculation is based on a mean PLFA concentration for a wide range of bacteria (15). We are currently improving this estimate by deriving a specific PLFA concentration for methanotrophic bacteria.. Few studies have made an assessment of methanotrophic biomass in terrestrial environments. Bender and Conrad (1, 2) used a direct most probable number technique to count methanotrophic bacteria from a wide range of different ...
Three UniSysCat research groups investigated in an interdisciplinary approach oxygen activation of a non-heme iron(II) Cyclam Center relevant for understanding the chemistry of dinuclear non-heme iron enzymes.
The classification of high-throughput sequencing data of protein-encoding genes is not as well established as for 16S rRNA. The objective of this work was to develop a simple and accurate method of classifying large datasets of pmoA sequences, a common marker for methanotrophic bacteria. A taxonomic system for pmoA was developed based on a phylogenetic analysis of available sequences. The taxonomy incorporates the known diversity of pmoA present in public databases, including both sequences from cultivated and uncultivated organisms. Representative sequences from closely related genes, such as those encoding the bacterial ammonia monooxygenase, were also included in the pmoA taxonomy. In total, 53 low-level taxa (genus-level) are included. Using previously published datasets of high-throughput pmoA amplicon sequence data, we tested two approaches for classifying pmoA: a naïve Bayesian classifier and BLAST. Classification of pmoA sequences based on BLAST analyses was performed using the lowest common
Stoichiometric Formation of an Oxoiron(IV) Complex by a Soluble Methane Monooxygenase Type Activation of O at an Iron(II)-Cyclam Center, D. Kass, T. Corona, K. Warm, B. Braun-Cula, U. Kuhlmann, E. Bill, S. Mebs, M. Swart, H. Dau, M. Haumann, P. Hildebrandt, K. Ray, Journal of the American Chemical Society 2020, 142, 5924-5928, 10.1021/jacs.9b13756 ...
Hery M, Singer AC, Kumaresan D, Bodrossy L, Sralis-Pavese, Prosser Jl, Thompson IP, Murrell JC (2008). Effect of earthworms on the community structure of active methanotrophic bacteria in landfill cover soil. ISME Journal 2, 92-104. ...
TY - JOUR. T1 - A Genome-Scale Metabolic Model for Methylococcus capsulatus (Bath) Suggests Reduced Efficiency Electron Transfer to the Particulate Methane Monooxygenase. AU - Lieven, Christian. AU - Petersen, Leander A. H.. AU - Jørgensen, Sten Bay. AU - Gernaey, Krist V.. AU - Herrgard, Markus J.. AU - Sonnenschein, Nikolaus. PY - 2018. Y1 - 2018. N2 - Genome-scale metabolic models allow researchers to calculate yields, to predict consumption and production rates, and to study the effect of genetic modifications in silico, without running resource-intensive experiments. While these models have become an invaluable tool for optimizing industrial production hosts like E. coli and S. cerevisiae, few such models exist for one-carbon (C1) metabolizers. Here we present a genome-scale metabolic model for Methylococcus capsulatus (Bath), a well-studied obligate methanotroph, which has been used as a production strain of single cell protein (SCP). The model was manually curated, and spans a total of ...
The particulate methane monooxygenase gene clusters, pmoCAB, from two representative type II methanotrophs of the alpha-Proteobacteria, Methylosinus trichosporium OB3b and Methylocystis sp, strain M, have been cloned and sequenced. Primer extension experiments revealed that the pmo cluster is probably transcribed from a single transcriptional start site located 300 bp upstream of the start of the first gene, pmoC, for Methylocystis sp, strain M, Immediately upstream of the putative start site, consensus sequences for sigma(70) promoters were identified, suggesting that these pmo genes are recognized by sigma(70) and negatively regulated under low-copper conditions. The pmo genes were cloned in several overlapping fragments, since parts of these genes appeared to be toxic to the Escherichia coil host. Methanotrophs contain two virtually identical copies of pmo genes, and it was necessary to use Southern blotting and probing with pmo gene fragments in order to differentiate between the two pmoCAB ...
Journal Publications. Choi, D.-W., R.C. Kunz, E.S. Boyd, J.D. Semrau, W.E. Antholine, J.-I. Han, J.A. Zahn, J.M. Boyd, A.M. de la Mora, and A.A. DiSpirito. 2003. The membrane-associated methane monooxygenase (pMMO) and pMMO-NADH:quinone oxidoreductase complex from Methylococcus capsulatus Bath. J. Bacteriol. 185: 5755 -5764.. Choi, D.-W, W. E. Antholine, Y.S. Do, Jeremy D. Semrau, C.J. Kisting, R.C. Kunz, D. Campbell, V. Rao, S.C. Hartsel, and A.A. DiSpirito. 2005. Effect of methanobactin on the activity and electron paramagnetic resonance spectra of the membrane associated methane monooxygenase on Methylococcus capsulatus Bath. Microbiology 151, 3417-3426.. Choi, D.-W, C.J. Zea, Y.S. Do, J.D. Semrau, W.E. Antholine, M.S. Hargrove, N.L. Pohl, E.S. Boyd, G. G. Geesey, S.C. Hartsel, P.H. Shafe, M.T. McEllistrem, C.J. Kisting, D. Campbell, V. Rao, A.M. de la Mora, and A.A. DiSpirito. 2006. Spectral, Kinetic, and thermodynamic properties of Cu(I) and Cu(II) binding by methanobactin from Methylosinus ...
A novel species, Methylocella tundrae, is proposed for three methanotrophic strains (T4T, TCh1 and TY1) isolated from acidic Sphagnum tundra peatlands. These strains are aerobic, Gram-negative, non-motile, dinitrogen-fixing rods that possess a soluble methane monooxygenase and utilize the serine pathway for carbon assimilation. Strains T4T, TCh1 and TY1 are moderately acidophilic organisms capable of growth between pH 4.2 and 7.5 (optimum 5.5-6.0) and between 5 and 30 degrees C (optimum 15 degrees C). The major phospholipid fatty acid is 18:1omega7c. The DNA G+C content of strain T4T is 63.3 mol%. The three strains possess almost identical 16S rRNA gene sequences and are most closely related to two previously identified species of Methylocella, Methylocella palustris (97% similarity) and Methylocella silvestris (97.5% similarity). DNA-DNA hybridization values of strain T4T with Methylocella palustris KT and Methylocella silvestris BL2T were respectively 27 and 36%. Thus, the tundra strains represent a
Carbon isotopic analysis of methane has become a popular technique in the exploration for oil and gas because it can be used to differentiate between thermogenic and microbial gas and can sometimes be used for gas-source rock correlations. Methane-oxidizing bacteria, however, can significantly change the carbon isotopic composition of methane; the origin of gas that has been partially oxidized by these bacteria could therefore be misinterpreted. We cultured methane-oxidizing bacteria at two different temperatures and monitored the carbon and hydrogen isotopic compositions of the residual methane. The residual methane was enriched in both 13C and D. For both isotopic species, the enrichment at equivalent levels of conversion was greater at 26??C than at 11.5??C. The change in ??D relative to the change in ??13C was independent of temperature within the range studied. One culture exhibited a change in the fractionation pattern for carbon (but not for hydrogen) midway through the...
Abstract: Soluble methane monooxygenase (sMMO) is a multicomponent metalloenzyme that catalyzes the conversion of methane to methanol at ambient temperature using a nonheme, oxygen-bridged dinuclear iron cluster in the active site. Structural changes in the hydroxylase component (sMMOH) containing the diiron cluster caused by complex formation with a regulatory component (MMOB) and by iron reduction are important for the regulation of O2 activation and substrate hydroxylation. Structural studies of metalloenzymes using traditional synchrotron-based X-ray crystallography are often complicated by partial X-ray-induced photoreduction of the metal center, thereby obviating determination of the structure of pure oxidation states. Here microcrystals of the sMMOH:MMOB complex from Methylosinus trichosporium OB3b were serially exposed to X-ray free electron laser (XFEL) pulses, where the ≦35 fs duration of exposure of an individual crystal yields diffraction data before photoreduction-induced ...
Methanobactin (Mb) from Methylosinus trichosporium OB3b is a member of a class of metal binding peptides identified in methanotrophic bacteria. Mb will selectively bind and reduce Cu(II) to Cu(I), and is thought to mediate the acquisition of the copper cofactor for the enzyme methane monooxygenase. These copper chelating properties of Mb make it potentially useful as a chelating agent for treatment of diseases where copper plays a role including Wilsons disease, cancers, and neurodegenerative diseases. Utilizing traveling wave ion mobility-mass spectrometry (TWIMS), the competition for the Mb copper binding site from Ag(I), Pb(II), Co(II), Fe(II), Mn(II), Ni(II), and Zn(II) has been determined by a series of metal ion titrations, pH titrations, and metal ion displacement titrations ...
The methanol dehydrogenase gene mxaF, encoding the large subunit of the enzyme, was amplified from the DNA of a number of representative methanotrophs, methyletrophs, and environmental samples by PCR using primers designed from regions of conserved amino acid sequence identified by comparison of three known sequences of the large subunit of methanol dehydrogenase. The resulting 550-bp PCR products were cloned and sequenced. Analysis of the predicted amino acid sequences corresponding to these mxaF genes revealed strong sequence conservation. Of the 172 amino acid residues, 47% were conserved among all 22 sequences obtained in this study. Phylogenetic analysis of these MxaF sequences showed that those from type I and type II methanotrophs form two distinct clusters and are separate from MxaF sequences of other gram-negative methylotrophs. MxaF sequences retrieved by PCR from DNA isolated from a blanket bog peat core sample formed a distinct phylogenetic cluster within the MxaF sequences of type ...
TY - JOUR. T1 - Recurrence and frequency of disturbance have cumulative effect on methanotrophic activity, abundance, and community structure.. AU - Ho, A.. AU - van den Brink, E.. AU - Reim, A.. AU - Krause, S.. AU - Bodelier, P.L.E.. N1 - 5979, ME; Data archiving: Data archived at MDA. PY - 2016. Y1 - 2016. N2 - Alternate prolonged drought and heavy rainfall is predicted to intensify with global warming. Desiccation-rewetting events alter the soil quality and nutrient concentrations which drive microbial-mediated processes, including methane oxidation, a key biogeochemical process catalyzed by methanotrophic bacteria. Although aerobic methanotrophs showed remarkable resilience to a suite of physical disturbances induced as a single event, their resilience to recurring disturbances is less known. Here, using a rice field soil in a microcosm study, we determined whether recurrence and frequency of desiccation-rewetting impose an accumulating effect on the methanotrophic activity. The response of ...
Rat liver microsomes act on 1,1-dichloro-cis -diphenylcyclopropane to form several products such as 2-chloro-3-oxo-1,3-diphenyl-1-propene. This involves an unusual type of ring fission which is considered to form 2,3-dichloro-1,3-diphenyl-1-propene as the initial product F573 . Methylococcus capsulatus soluble methane monooxygenase (E.C. 1.14.13.25) converts cyclopropylbenzene into benzyl alcohol. 3-Phenylprop-2-en-1-ol is also formed, and this may be an intermediate F278 . 1.6 Reactions.... ...
Lineage: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales; Methylococcaceae; Methylomonas; Methylomonas ...
ID A0A177P5V1_9GAMM Unreviewed; 251 AA. AC A0A177P5V1; DT 07-SEP-2016, integrated into UniProtKB/TrEMBL. DT 07-SEP-2016, sequence version 1. DT 27-SEP-2017, entry version 12. DE RecName: Full=Flagellar brake protein YcgR {ECO:0000256,HAMAP-Rule:MF_01457}; DE AltName: Full=Cyclic di-GMP binding protein YcgR {ECO:0000256,HAMAP-Rule:MF_01457}; GN Name=ycgR {ECO:0000256,HAMAP-Rule:MF_01457}; GN ORFNames=A1356_13480 {ECO:0000313,EMBL:OAI25472.1}; OS Methylomonas koyamae. OC Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales; OC Methylococcaceae; Methylomonas. OX NCBI_TaxID=702114 {ECO:0000313,EMBL:OAI25472.1, ECO:0000313,Proteomes:UP000077734}; RN [1] {ECO:0000313,EMBL:OAI25472.1, ECO:0000313,Proteomes:UP000077734} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=R-49807 {ECO:0000313,EMBL:OAI25472.1, RC ECO:0000313,Proteomes:UP000077734}; RA Ploux O.; RL Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Acts as a flagellar brake, regulating swimming and ...
Fisher, O. S.; Sendzik, M. R.; Ross, M. O.; Lawton, T. J.; Hoffman, B. M.; Rosenzweig, A. C. PCuAC domains from methane-oxidizing bacteria use a histidine brace to bind copper. J. Biol. Chem. 2019, 294, 16351.. Kenney, G. E.; Dassama, L. M. K.; Manesis, A. C.; Ross, M. O.; Chen, S.; Hoffman, B. M.; Rosenzweig, A. C. MbnH is a diheme MauG-like protein associated with microbial copper homeostasis. J. Biol. Chem. 2019, 294, 16151. Ro, S. Y.; Schachner, L. F.; Koo, C. W.; Purohit, R.; Remis, J. P.; Kenney, G. E.; Liauw, B. W.; Thomas, P. M.; Patrie, S. M.; Kelleher, N. L.; Rosenzweig, A. C. Native top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase. Nat. Commun. 2019, 10, 2675.. Ross, M. O.; MacMillan, F.; Wang, J.; Nisthal, A.; Lawton, T. J.; Olafson, B. D.; Mayo, S. L.; Rosenzweig, A. C.; Hoffman, B. M. Particulate methane monooxygenase contains only monocopper centers. Science 2019, 364, 566-570.. Ross, M. O.; Fisher, O. S.; Morgada, M. N.; ...
Abstract: A binary metallic catalyst (PtSn/C) and a ternary metallic catalyst (PtSnCo/C) with a metal mass fraction of 20% were prepared by borohydride reduction and subsequent hydrothermal treatment in a glycol liquid phase. The structure and composition of the as-prepared electrocatalysts were characterized by X-ray diffraction (XRD) and energy-dispersive spectrometry (EDS). Their activity and stability for the catalysis of methanol oxidation were evaluated by anodic linear sweep voltammetry (LSV), cyclic voltammetry (CV), and the anodic stripping of a pre-adsorbed CO monolayer. We found that the PtSnCo/C catalyst gave the best catalytic activity for the methanol oxidation of all the catalysts including the commercial JM-PtRu/C catalyst. After 100 cycles, the peak current of methanol oxidation for the PtSn/C catalyst rapidly decreased to 11% of its initial peak current but PtSnCo/C decreased to only 50%. This result suggests that the PtSnCo/C catalyst has better chemical stability for the ...
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Over the past few months, reporting stations around the Arctic have shown a ramping rate of atmospheric methane accumulation. The curves in the graphs are steepening, hinting at a growing release of methane from a warming Arctic environment. * * * * (Alert, Canada methane graph shows atmospheric methane increases in the range of 20…
Over the past few months, reporting stations around the Arctic have shown a ramping rate of atmospheric methane accumulation. The curves in the graphs are steepening, hinting at a growing release of methane from a warming Arctic environment. * * * * (Alert, Canada methane graph shows atmospheric methane increases in the range of 20…
Active methanotrophs in a landfill soil were revealed by detecting the 16S rRNA of methanotrophs and the mRNA transcripts of key genes involved in methane oxidation. New 16S rRNA primers targeting type I and type II methanotrophs were designed and optimized for analysis by denaturing gradient gel electrophoresis. Direct extraction of RNA from soil enabled the analysis of the expression of the func ...
Active methanotrophs in a landfill soil were revealed by detecting the 16S rRNA of methanotrophs and the mRNA transcripts of key genes involved in methane oxidation. New 16S rRNA primers targeting type I and type II methanotrophs were designed and optimized for analysis by denaturing gradient gel electrophoresis. Direct extraction of RNA from soil enabled the analysis of the expression of the func ...
In this activity, students graph and analyze methane data, extracted from an ice core, to examine how atmospheric methane has changed over the past 109,000 years in a case study format. Calculating the rate of change of modern methane concentrations, they compare the radiative forcing of methane and carbon dioxide and make predictions about the future, based on what they have learned from the data and mans role in that future.. ...
Current estimates of permafrost soil carbon stocks are 1670 PgC (Tarnocai et al., 2009), the single largest component of the terrestrial carbon pool and higher than previously thought. Terrestrial carbon models show a land CO2 sink with warming at high northern latitudes, however none of the models participating in C4MIP or CMIP5 included explicit representation of permafrost soil carbon decomposition, which at a minimum requires sufficient vertical resolution in modelled soil carbon distribution and processes to separate surface pools from very old (Pleistocene) permafrost carbon pools. Including permafrost carbon processes into an ESM can change the sign of this C response to warming from a sink to a source in northern high latitudes (Koven et al., 2011). The magnitude of this source of CO2 to the atmosphere from decomposition of permafrost carbon varies widely by 2100 according to different model estimates: process-model estimates include 7-17 Pg (Zhuang et al., 2006), 55-69 Pg (Koven et al., ...
Bifunctional Anode Catalysts for Direct Methanol Fuel Cells, J. Rossmeisl, P. Ferrin, G. A. Tritsaris, A. U. Nilekar, S. Koh, S. E. Bae, S. R. Brankovic, P. Strasser, M. Mavrikakis, Energy & Environmental Science 5, 8335 (2012). [DOI] ...
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Please take a moment to fill out a short, 5 minute evaluation of the Global Methane Forum. Your feedback will help determine how GMI plans its future meetings.. The Global Methane Initiative (GMI) and the Climate and Clean Air Coalition (CCAC) were pleased to welcome more than 500 attendees from 62 countries to Washington, DC for the Global Methane Forum and CCAC Working Group meetings. Methane experts and policy-makers from around the world gathered to discuss options for methane reduction and abatement activities.. This unique opportunity granted access to:. ...
Constance Chu, MD is part of Stanford Profiles, official site for faculty, postdocs, students and staff information (Expertise, Bio, Research, Publications, and more). The site facilitates research and collaboration in academic endeavors.
WASHINGTON (AP) - The Interior Department said Thursday it is delaying an Obama-era regulation aimed at restricting harmful methane emissions from oil and gas production on federal lands.
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Its a small town, so everyone knows. There was a trial and national press, but a couple loud conversations at the bank spread the word just as well.
This week on The MMO Report, Casey discusses the un-disbanding of the Multiverse project and associated Firefly/Buffy IPs, Wakfus bright and bizarre charact...
鎂是人體內重要元素之一,是體內礦物質含量第四豐富的陽離子,僅次於鈣、鉀、鈉,在體液中主要以二價陽離子形式存在。成年人體內鎂總量約21-28克,鎂主要存在於細胞內,它在細胞內液的含量僅次於鉀,是細胞內的重要陽離子。其中骨骼肌佔27%,其他細胞佔6%-7%(以肝臟為最高)中,細胞外液(extracellular fluid,ECF)約佔1%,其餘60%-65%集中在骨骼,占骨頭總灰分的0.5%~0.7%。骨骼的鎂約三分之一與磷酸根緊密結合,其餘三分之二吸附於骨骼表面,可用來維持血液和組織的正常濃度。軟組織的鎂含量約為每公斤2.5-9毫莫耳,與代謝活性成正比。紅細胞內濃度2.5mmo1/L,血清 0.75-1.25 mmo1/L。血漿中鎂濃度平均約為0.85 mM (0.7-1.0 mM),其中60%為離子態,30%與白蛋白(albumin)結合,10%為小分子複合物。細胞內鎂約90 ...
Methanes Contribution to Global Warming Is Worse than You Thought It seems weve been fudging the numbers in a sense, when comparing the global warming pote…
Product Name: Proparyl-PEG5-methaneFormula: C12H22O5MW: 246.3Web Site:MedchemexpressPurity: 0.98Availability: In StockCAS NO: 1345675-02-6 Product: ETP-46464 S
In the end, MA will either have more conservative representation in the Senate for the next epoch and will have wasted millions of campaign $ and hours, or it will have the same representation and will have wasted millions of campaign $ and hours. Thanks @joekennedy! # ...
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D-arabino-3-Hexulose 6-phosphate was prepared by condensation of formaldehyde with ribulose 5-phosphate in the presence of 3-hexulose phosphate synthase from methane-grown Methylococcus capsulatus. The 3-hexulose phosphate was unstable in solutions of pH greater than 3, giving a mixture of products in which, after dephosphorylation, allulose and fructose were detected. A complete conversion of d-ribulose 5-phosphate and formaldehyde into d-fructose 6-phosphate was demonstrated in the presence of 3-hexulose phosphate synthase and phospho-3-hexuloisomerase (prepared from methane-grown M. capsulatus). d-Allulose 6-phosphate was prepared from d-allose by way of d-allose 6-phosphate. No evidence was found for its metabolism by extracts of M. capsulatus, thus eliminating it as an intermediate in the carbon assimilation process of this organism. A survey was made of the enzymes involved in the regeneration of pentose phosphate during C1 assimilation via a modified pentose phosphate cycle. On the basis ...
Bacteriohopanepolyols (BHPs) are bacterial biomarkers with a likely potential to identify present and past methanotrophic communities. To unravel the methanotrophic community in peat bogs, we report the BHP signatures of type I and type II methanotrophs isolated from Sphagnum mosses and of an extreme acidophilic verrucomicrobial methanotroph. A type I Methylovulum-like strain (M200) contains a remarkable combination of BHPs, including a complete suite of mono-unsaturated aminobacteriohopanepentol, -tetrol and -triol. The Methylomonas-like strain (M5) mainly produces aminobacteriohopanepentol, characteristic for type I methanotrophs, and the Methylosinus-like strain (29) contains both aminobacteriohopanetetrol and aminobacteriohopanetriol, typical for a type II methanotroph. The type II methanotroph Methylocella palustris and the verrucomicrobial Methylacidiphilum fumariolicum strain SolV primarily produce aminotriol, which is also produced by many other bacteria. In Sphagnum mosses and ...
1MTY: Crystal structures of the methane monooxygenase hydroxylase from Methylococcus capsulatus (Bath): implications for substrate gating and component interactions.
Bioremediation is a promising and cost-effective technology widely used to clean up waste containing organic or inorganic contaminants. Cr(VI), the highest oxidation state of the metal chromium, is widely used in various industries, and is extremely toxic to animals and humans. Utilisation of chromium (Cr) reducing microbes and their products can enhance the efficiency of the process of detoxification of Cr(VI) to Cr(III).This study was conducted to investigate the microbial reduction of chromium [Cr(VI)] in general and the potential for biological treatment of Cr(Vl)-containing wastes in particular. Cr(VI) was transformed to non-toxic Cr(III) by Escherichia coli ATCC 33456. It was observed for the first time that the type I methanotrophic bacterium Methylococcus capsulatus (Bath) is able to reduce chromium (VI) to chromium (III), using methane as the carbon and energy source. The reaction occurred over a wide range of chromium (VI) concentrations (10-1,000 mg/1). The reaction was studied by a ...
Methane is a flammable gas that is the main component of natural gas. It is a highly potent greenhouse gas, and accounts for about 20% of greenhouse gas emissions. Methane is routinely flared in many industrial processes without harnessing any of its energy. The environmental impact and wasted energy potential make it highly desirable to find an economically feasible process to use this methane.One possibility is to convert methane into liquid fuels for transportation and energy generation. Current technologies to convert methane gas to liquid fuels (GTL) are complex, and the facilities are only economical at huge scales. Methane gas is very difficult to transport and store, so GTL plants must be located at the source of the methane, typically at large petroleum fields or refineries.Biological conversion of methane to liquid fuels is an attractive alternative to traditional GTL processes, as microbial oxidation of methane can produce liquid fuels (e.g. methanol) at ambient temperatures and ...
Methane utilization by methanotrophic bacteria is an attractive application for biotechnological conversion of natural or biogas into high-added-value products. Haloalcaliphilic methanotrophic bacteria belonging to the genus Methylomicrobium are among the most promising strains for methane based biotechnology, providing easy and inexpensive cultivation, rapid growth, and the availability of established genetic tools. A number of methane bioconversions using these microbial cultures have been discussed, including the derivation of biodiesel, alkanes, and OMEGA-3 supplements. These compounds are derived from bacterial fatty acid pools. Here, we investigate fatty acid biosynthesis in Methylomicrobium buryatense 5G(B1). Most of the genes homologous to typical Type II fatty acid biosynthesis pathways could be annotated by bioinformatics analyses, with the exception of fatty acid transport and regulatory elements. Different approaches for improving fatty acid accumulation were investigated. These ...
NORMAN, OKLA. - A University of Oklahoma-led study shows that paddy rice (both area and plant growth) is significantly related to the spatial-temporal dynamics of atmospheric methane concentration in monsoon Asia, where 87% of paddy rice fields are situated in the world.. Methane is one of the major greenhouse gases. It has a lifetime of 12.4 years and its global warming potential is approximately 86 times higher than carbon dioxide over a 20-year period.. Rice paddy is a large source of methane emission; however, it has been a challenging task to attribute relative role of rice paddy in the spatial distribution, seasonal dynamics and interannual variation of atmospheric methane concentration as measured by spaceborne sensors, said Xiangming Xiao, a member of the Earth Observation and Modeling Facility at OU and a professor in the Department of Microbiology and Plant Biology who coordinated this interdisciplinary study.. Over the past few years, researchers at OU developed annual paddy rice ...
The currently accepted thesis on nitrogenous fertilizer additions on methane oxidation activity assumes niche partitioning among methanotrophic species, with activity responses to changes in nitrogen content being dependent on the in situ methanotrophic community structure Unfortunately, widely applied tools for microbial community assessment only have a limited phylogenetic resolution mostly restricted to genus level diversity, and not to species level as often mistakenly assumed. As a consequence, intragenus or intraspecies metabolic versatility in nitrogen metabolism was never evaluated nor considered among methanotrophic bacteria as a source of differential responses of methane oxidation to nitrogen amendments. We demonstrated that fourteen genotypically different Methylomonas strains, thus distinct below the level at which most techniques assign operational taxonomic units (OTU), show a versatile physiology in their nitrogen metabolism. Differential responses, even among strains with identical 16S
Methanotrophic bacteria utilize methane as their sole source of carbon and energy. The first step in their metabolic pathway is the oxidation of methane to meth...
1FYZ: Crystal structures of the soluble methane monooxygenase hydroxylase from Methylococcus capsulatus (Bath) demonstrating geometrical variability at the dinuclear iron active site.
Methanol oxidation catalysts comprising an outer Pt-shell with an inner Ni-core supported on carbon, (Pt-Ni/C), were prepared with either crystalline or amorphous Ni core structures. Structural comparisons of the two forms of catalyst were made using transmission electron microscopy (TEM), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS), and methanol oxidation activity compared using CV and chronoamperometry (CA). While both the amorphous Ni core and crystalline Ni core structures were covered by similar Pt shell thickness and structure, the Pt-Ni(amorphous)/C catalyst had higher methanol oxidation activity. The amorphous Ni core thus offers improved Pt usage efficiency in direct methanol fuel cells.
Methane availability is rising as the extraction of natural gas booms, and more methane is escaping into the atmosphere. Methanotrophs are the primary biological mechanism for mitigating the release of methane by consuming it for carbon and energy. These organisms also have great potential in the biotechnological utilisation of methane, a readily renewable carbon source, for the production of bulk and fine chemicals and sustainable energy.. To oxidise methane, methanotrophs use an enzyme called methane monooxygenase whose essential cofactor is copper (some can also use iron). Understanding how methanotrophs handle copper is therefore of great importance for all potential applications of these organisms.. The scientists describe the discovery and characterisation of Csp1 from a methanotroph that can bind large quantities of copper and propose this is a protein that accumulates copper for methane oxidation.. Lead author Chris Dennison (pictured), Professor of Biological Chemistry at Newcastle ...
Fingerprint Dive into the research topics of Mechanism of the methane → methanol conversion reaction catalyzed by methane monooxygenase: A density functional study. Together they form a unique fingerprint. ...
Researchers from Nijmegen in the Netherlands and Naples, found Acidomethylosilex fumarolicum in hot, acidic volcano mudpots in Italy. This bacterium can thrive in extremely acidic conditions, down to pH 0.8, and its preferred temperature is around 55 deg. C. Surprisingly, it is unrelated to any of the known methanotrophic bacteria, which are all found in one of two large phylogenetics groups, the alpha and the gamma proteobacteria. A. fumarolicum appears to belong to the phylum verrucomicrobia, only very distantly related to proteobacteria. The microbe hunters tracked it down by looking for a key enzyme of methane metabolism, methane mono-oxygenase. Eventually they found three copies of the corresponding gene in A. fumarolicum, but they were quite different from the ones in known methane eaters, explaining why the extreme bug hasnt been found before ...
Proteobacteria capable of converting the greenhouse gas methane to biomass, energy, and carbon dioxide represent a small but important sink in global methane inventories. Currently, 23 genera of methane oxidizing (methanotrophic) proteobacteria have been described, although many are represented by only a single validly described species. Here we describe a new methanotrophic isolate that shares phenotypic characteristics and phylogenetic relatedness with the marine methanotroph Methylomarinum vadi. However, the new isolate derives from a terrestrial saline mud pot at the northern terminus of the Eastern Pacific Rise (EPR). This new cultivar expands our knowledge of the ecology of Methylomarinum, ultimately towards a fuller understanding of the role of this genus in global methane cycling.
Membranes form the structural and functional limits of the cell and are the site of many essential functions such as energy transduction and the initiation of responses to environmental stimuli. My lab has studied the molecular events involved in membrane development using two model systems - phototrophic bacteria and methanotrophic bacteria. Unlike most other bacteria, both of these groups form an intracytoplasmic membrane (ICM) the formation of which is affected by environmental conditions thus allowing laboratory manipulation of membrane development. Methanotrophic bacteria use methane as a sole source of carbon and energy. The role of the ICM in the metabolism of one carbon compounds is evidenced by the localization of enzymes of methane metabolism to the ICM. The photosynthetic apparatus of Rhodospirillum rubrum is housed in an ICM. Using biochemical, immunochemical, and ultrastructural approaches, work from my laboratory has demonstrated that the ICM and cytoplasmic membrane are continuous ...
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Read Electro-catalytic performance of Pt-supported poly (o-phenylenediamine) microrods for methanol oxidation reaction, Research on Chemical Intermediates on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Methane emissions due to human-related activities, shown to the right of the volcano, account for approximately 70 per cent of the total emissions (Reeburgh 2007; Colwell and Ussler III 2010). Gas Hydrates are currently estimated to contribute about 5 Tg (Tg = 1012 grammes) of methane (3.5 Tg carbon) per year to the atmosphere (IPCC, 2007). Emission rates for gas hydrates are highly speculative, however, and may overestimate the atmospheric methane contribution by not fully accounting for methane consumed by microbes in the sediment and water column. Additional research will be required to constrain the methane flux from gas hydrates that reaches the atmosphere. For any form of publicat...
Get information, facts, and pictures about Council of Constance at Encyclopedia.com. Make research projects and school reports about Council of Constance easy with credible articles from our FREE, online encyclopedia and dictionary.
A rise in atmospheric methane emissions after 2006 is likely due to expanding wetlands and an increase in emissions from mining of fossil fuels, say researchers.
Analysis of samples from 2020 also showed a significant jump in the atmospheric burden of methane, which is far less abundant but 28 times more potent than CO2 at trapping heat over a 100-year time frame. NOAAs preliminary analysis showed the annual increase in atmospheric methane for 2020 was 14.7 parts per billion (ppb), which is the largest annual increase recorded since systematic measurements began in 1983. The global average burden of methane for December 2020, the last month for which data has been analyzed, was 1892.3 ppb. That would represent an increase of about 119 ppb, or 6 percent, since 2000.. In early April, GML typically releases a preliminary estimate of the global annual atmospheric increase for key greenhouse gases from January 1st in one year to January 1st in the next year. This preliminary estimate is based on measurements from weekly air samples collected at about 40 sites around the world. While GMLs preliminary estimates are typically a little higher than the final ...
I am a New Age monitor of this blog. Indian Country Today Media Network, the worlds largest Indian/Indigenous news source, occasionally posts a selective comment or two, rarely three, on its articles. It regularly posts my comments on a world-renowned indigenous peoples rights activists articles. The url to my New Age website is included in some of my comments. One of my most recent comments reminds me of your [or Constance Cumbeys] book Hidden Dangers of the Rainbow. My comment reads. Good article Mr. ... I believe that the solution is the assimilation of the entire human race into the New Age retribalization of the world movement. On my website, located at http://www.towahkon.org/Tomssite.html, I first present a short summary of my indigenous peoples rights advocacy work and related hippie countercultural New Age mission. At the end of the summary I have news statements related to the summary. One of the news statements reads: On Aug. 12, 2016, I sent a family letter to my uncles and ...
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Constance Mao, M.D. is a UW associate professor in the Department of Obstetrics and Gynecology who joined the department in 1997. She is director of the…
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Friedrichshafen: Friedrichshafen, city, Baden-Württemberg Land (state), southwestern Germany. It lies on the north shore of Lake Constance (Bodensee), about 50 miles (80 km) northeast of
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Chen, Hsiang-Sheng; Benedetti, Tania M.; Lian, Jiaxin; Cheong, Soshan; OMara, Peter B. et al. (2021). Role of the Secondary Metal in Ordered and Disordered Pt-M Intermetallic Nanoparticles: An Example of Pt3Sn Nanocubes for the Electrocatalytic Methanol Oxidation. ACS Catalysis 11(4) , 2235-2243. 10.1021/acscatal.0c05370. ...
The US Environmental Protection Agency (EPA) has proposed updates to oil and gas regulations that would remove restrictions on methane emissions.
ഒരിക്കൽ മാർഗരറ്റ്, ക്ലാർക്ക് യൂണിവേഴ്സിറ്റിയിൽ നിന്നും ലിയോനാർഡ് ബ്ലെയിൻ നൈസുമായി (Leonard Blaine Nice) കൂടിക്കാഴ്ച നടത്തുകയുണ്ടായി. തുടർന്ന് 1908 -ൽ അവർ വിവാഹിതരായി. ഇവർക്ക് അഞ്ചു കുട്ടികൾ ജനിച്ചു. 1911 ഇൽ കോൺസ്റ്റൻസ് (Constance), 1912 ഇൽ മാർജറി (Marjorie), 1916 -ഇൽ ബാർബറ (Barbara), 1918-ഇൽ എലീനോർ ( Eleanor), 1922 ഇൽ ജനിച്ച ജാനെറ്റ് (Janet) എന്നിവരായിരുന്നു മക്കൾ. ഇവരിൽ ഒഹായോയിലെ കൊളംബസിൽ വെച്ച് ഒമ്പതാം വയസ്സിൽ ന്യുമോണിയ ബാധിച്ച് ...
E ai pessoal, tudo blz? Montei um question rio para coletar dados sobre o que motiva os players a jogarem um MMO. Gostaria muito que a comunidade da
A mixture of ethyne ( C2H2 ) and methane ( CH4 ) occupied a certain volume at a total pressure of 16.8 kPa. Upon burning the sample to form CO2 and H2O, the CO2 was collected and its pressure found to be 25.6 kPa in the same volume and at the same temperature as the original mixture. What percent of the original mixture was methane? ...
RealClimate: Ive put together an easy-to-play-with online model of methane in the atmosphere. Im going to use it for teaching along with the rest of the Understanding the Forecast webmodels, but it was designed to be relevant to the issue of abrupt new methane burps as weve been ruminating about lately on Realclimate. The model runs […]
Læs mere om New Holland [Product] T6 METHANE POWER serien, kig i galleriet, gennemgå de tekniske specifikationer eller find en forhandler.
NMED, EMNRD hosted meetings as the state works to develop methane emission regulations. Two readers say the state needs strong regulations.
Directed by Moody Shoaibi. With Mark Tonderai, Nathan Constance, David Oyelowo, Crunski. A kidnap caper of a dog to make some much needed cash to pay off some outstanding debts goes wrong...with not so hilarious consequences.
When you're stressed and in a jam, Constance C.R. White says, "So what?" That phrase keeps life's challenges in their proper context.
The long-rumored new installment of Blizzards massive StarCraft series is set to be unveiled later this month - and its another massively-multiplayer
CBD is an abbreviation for cannabidiol, a natural cannabinoid found in the cannabis plant. This substance has been subject to much curiosity from medical researchers and scientists. CBD naturally interacts with the human bodys endocannabinoid system, which consists of cannabinoid receptors throughout the body. CBD has become a point of interest due to its potential therapeutic effects without psychoactive effects. CBDs cousin THC has potential therapeutic effects, but people are wary of the psychoactive effects.. CBD is isolated and extracted from varieties of the cannabis plant. Usually, CBD comes from hemp, which contains high levels of CBD but nearly no THC. Most of the CBD used for medical or dietary purposes comes from the hemp plant. Current research presents a variety of different potential benefits for humans. In the future, we may see CBD become a normal part of our everyday lives.. As we mentioned cannabidiol, is a substance made of cannabinoids, is derived from the cannabis plants. ...
... s in Gammaproteobacteria are known from family Methylococcaceae. Methanotrophs from Alphaproteobacteria are found ...
... is a bacterial genus from the family of Methylococcaceae. "Methylohalobius". List of Prokaryotic names with ...
Methylococcaceae, a family of microbes found in the Fourth Street Basin, consume methane. Desulfobacterales take in sulfate and ...
... have been observed with the presence of gammaproteobacteria in the Methylococcaceae lineages. Methanotrophs convert methane ... major symbionts Allochromatium Thiomicrospira Thioalkalivibrio Methylococcaceae Beggiatoa Thioploca Deltaproteobacteria - ...
... which are structurally and biochemically distinct from the Methylococcaceae or type I methanotrophs. In this family methane is ...
... methylococcaceae MeSH B03.440.400.425.500.500 - Methylococcus MeSH B03.440.400.425.500.500.100 - Methylococcus capsulatus MeSH ... methylococcaceae MeSH B03.660.250.500.500 - Methylococcus MeSH B03.660.250.500.500.100 - Methylococcus capsulatus MeSH B03.660. ...
The Methylococcaceae are a family of bacteria that obtain their carbon and energy from methane, called methanotrophs. They ... The Methylococcaceae have internal membranes in the form of flattened discs, perpendicular to the cell wall. Methane is ... Springer, New York, 2005, Volume 2: The Proteobacteria, Part B: The Gammaproteobacteria Methylococcaceae J.P. Euzéby: List of ... Some species of the Methylococcaceae have formed with certain marine mussels endosymbiotic relationships. UniProt Madigan MT, ...
The Methylococcaceae are a family of bacteria that obtain their carbon and energy from methane, called methanotrophs. They ... The Methylococcaceae have internal membranes in the form of flattened discs, perpendicular to the cell wall. Methane is ... Springer, New York, 2005, Volume 2: The Proteobacteria, Part B: The Gammaproteobacteria Methylococcaceae J.P. Euzéby: List of ... Some species of the Methylococcaceae have formed with certain marine mussels endosymbiotic relationships. UniProt Madigan MT, ...
Family Methylococcaceae. Warning: In the List of Prokaryotic names with Standing in Nomenclature, an arrow (→) only indicates ... n. Methylococcaceae, the Methylococcus family. Valid publication: Validation List no. 15. Int. J. Syst. Bacteriol., 1984, 34, ... Methylococcaceae Whittenbury and Krieg 1984 emend. Bowman et al. 1993. Type genus: ¤ Methylococcus Foster and Davis 1966 ( ... n. Methylococcaceae, the Methylococcus family. Reference: BOWMAN (J.P.), SLY (L.I.), NICHOLS (P.D.) and HAYWARD (A.C.): Revised ...
Methanotrophs in Gammaproteobacteria are known from family Methylococcaceae. Methanotrophs from Alphaproteobacteria are found ...
Bowman J (2006) The methanotrophs - the families Methylococcaceae and Methylocystaceae. In: Dworkin M, Falkow S, Rosenberg E, ... Methanotrophic bacteria Methane monooxygenase Methylococcaceae Methylothermaceae Methylocystaceae Beijerinckiaceae ... Bowman JP (2016a) Methylococcaceae. In: Bergeys manual of systematics of archaea and bacteria, Online © 2016 Bergeys Manual ...
The metagenomic analysis revealed that Methylococcaceae and Methylophilaceae families were the most abundant in both ... The metagenomic analysis revealed that Methylococcaceae and Methylophilaceae families were the most abundant in both ... Methylophilaceae and Methylococcaceae were the most abundant families in both samples (6.390 ± 4.132 and 8.970 ± 4.086% for ... The metagenomic analysis revealed that Methylococcaceae and Methylophilaceae families were the most abundant in both ...
OC Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales; OC Methylococcaceae; Methylomonas. OX NCBI_TaxID=702114 {ECO ...
PEGs related to methane monooxygenase of Methylococcaceae were also detected, but at very low abundance (Fig. 1). The metabolic ... 5 for Methylococcaceae (class γ-proteobacteria); 6 for Brocadiaceae (phylum Planctomycetes); 7 for Desulfobulbaceae and ...
Family Methylococcaceae [IV] *Genus Methylococcus [I]. *Genus Methylomonas [II]. *Family Neisseriaceae [VIII] *Genus ...
2009), who proposed that Crenothrix could be a member of the Methylococcaceae, based on its 16S rRNA gene phylogeny (Figure 1). ... Bowman, J. P., Sly, L. I., and Stackebrandt, E. (1995). The phylogenetic position of the family Methylococcaceae. Int. J. Syst ... Whittenbury, R., and Krieg, N. R. (1984). "Family Methylococcaceae," in Bergeys Manual of Systematic Bacteriology, eds N. R. ... They are present in further Methylococcaceae strains, which are distantly related to the known genera but have so far not been ...
The phylogenetic position of the family Methylococcaceae. Int. J. Syst. Bacteriol. 45: 182-185, 1995. PubMed: 7857800 ... Erratum: The phylogenetic position of the family Methylococcaceae. Int. J. Syst. Bacteriol. 45: 622, 1995. ... and aproposal that the family Methylococcaceae includes only the Group I methanotrophs. Int. J. Syst. Bacteriol. 43: 735-753, ...
Lineage: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales; Methylococcaceae; Methylomonas; ...
Methylococcaceae. - Oceanospirillaceae. - Alcanivoraceae. - Hahellaceae and Litoricolaceae. - Halomonadaceae. - ...
... with the Crenotrichaceae and Methylococcaceae being most abundant. Dominant taxa within the mixed cave water were affiliated to ... Methane release from sediment seeps to the atmosphere is counteracted by highly active Methylococcaceae in the water column of ... Members of the Methylococcaceae and Methylophilaceae have been previously reported to trophically interact in methane-fueled ... A metagenomic insight into freshwater methane-utilizing communities and evidence for cooperation between the Methylococcaceae ...
Whittenbury R and Krieg NR (1989) Methylococcaceae. In: Staley JT, Bryant MP, Pfennig N and Holt JG (eds) Bergeys Manual of ...
Methanotrophs are organisms that require methane as a source of carbon and energy for their metabolism. They are gram-negative bacteria that are capable in utilizing methane as a carbon energy source and able to grow both aerobically or anaerobically which only need single-carbon compound to live on. These prokaryotes occur mostly in soils, rice paddles, mud, landfills, and other places where methane is available. They can grow aerobically or anaerobically. In aerobic conditions, they use oxygen and methane to produce formaldehyde. They then incorporate it into organic compounds through ribulose monophosphate pathway or by serine pathway. An example of methanotroph is a gammaproteobacterium that utilize RuMP pathway in assimilating carbon. Such bacteria are referred to as type I methanotrophs. Type II methanotrophs are those from alphaproteobacteria that utilize the serine pathway of carbon assimilation. They have a system of internal membranes where methane oxidation occurs.1 Methanotrophs are ...
Methylococcaceae ( jstor ). Microbiology ( jstor ). Nutrients ( jstor ). Oxidation ( jstor ). Oxygen ( jstor ). Record ...
Novel Methanotrophs of the Family Methylococcaceae from Different Geographical Regions and Habitats. Microorganisms. ...
The methanotrophs-the families Methylococcaceae and Methylocystaceae, p 266-289. In Dworkin M, Falkow S, Rosenberg E, Schleifer ... The relative abundance of SSU rRNA assigned to the methane-oxidizing bacteria (MOB) within Methylococcaceae increased with ... The relative abundances of 16S rRNA and mRNA assigned to the methanotrophic Methylococcaceae, primarily Methylobacter, ... within the Methylococcaceae, mRNA sequence tags were not assigned further to genus level taxonomy. The majority of mRNA was ...
Appearance of Methylococcaceae strain SSMP-1 when grown on solid medium.. Colonies required approximately 2 weeks to become ... Motility in Methylococcaceae strain SSMP-1, video loop.. Actively growing cells demonstrate clear tumbling motility in wet- ... Analysis of the 16S rRNA gene supports placement of Methylococcaceae strain SSMP-1 near Methylomarinum vadi strains IT4 and T2- ... Methylomarinum vadi (Gammaproteobacteria, family Methylococcaceae) was isolated in 2007 from a marine mat associated with a ...
Ammonia oxidizers (family Nitrobacteraceae) and methanotrophs (family Methylococcaceae) oxidize CO and CH4 to CO2 and NH4+ to ...
Communal metabolism by Methylococcaceae and Methylophilaceae is driving rapid aerobic methane oxidation in sediments of a ...
Family Methylococcaceae Methylobacter Methylocaldum Methylococcus Methylogaea Methyloglobulus Methylohalobius Methylomagnum ...
Communal metabolism by Methylococcaceae and Methylophilaceae is driving rapid aerobic methane oxidation in sediments of a ...
Communal metabolism by Methylococcaceae and Methylophilaceae is driving rapid aerobic methane oxidation in sediments of a ...
The relative abundances of 16S rRNA and mRNA assigned to the methanotrophic Methylococcaceae, primarily Methylobacter, ...
High Throughput Sequencing to Detect Differences in Methanotrophic Methylococcaceae and Methylocystaceae in Surface Peat, ...
... and DNA-SIP identified members of the methanotrophic family Methylococcaceae as major 13CH 4 consumers. Members of ... and DNA-SIP identified members of the methanotrophic family Methylococcaceae as major 13CH4 consumers. Members of ...
With the notable exception of members of the family Methylococcaceae, bacteria do not produce sterols, but they do produce ...
A metagenomic insight into freshwater methane-utilizing communities and evidence for cooperation between the Methylococcaceae ...
Tsednee, M., Castruita, M., Salomé, P. A., Sharma, A., Lewis, B. E., Schmollinger, S. R., Strenkert, D., Holbrook, K., Otegui, M. S., Khatua, K., Das, S., Datta, A., Chen, S., Ramon, C., Ralle, M., Weber, P. K., Stemmler, T. L., Pett-Ridge, J., Hoffman, B. M. & Merchant, S. S., Jan 1 2019, In : Journal of Biological Chemistry. 294, 46, p. 17626-17641 16 p.. Research output: Contribution to journal › Article ...
  • The Methylococcaceae are a family of bacteria that obtain their carbon and energy from methane, called methanotrophs. (wikipedia.org)
  • The metagenomic analysis revealed that Methylococcaceae and Methylophilaceae families were the most abundant in both communities, moreover, it strongly suggest that backbones of both mats were formed by filamentous bacteria, such as Leptothrix, Thiothrix, and Beggiatoa. (frontiersin.org)
  • Methanogens belonging to family Methanobacteriaceae and methanotrophic bacteria belonging to family Methylococcaceae were heavily enriched in three ultrabasic springs (pH 12). (peerj.com)
  • The bacteria that consumed methane were a group of bacteria called Methylococcaceae -- the same bacteria that were abundant in September after the methane had been consumed, suggesting that they were, in fact, important in consuming methane. (ucsb.edu)
  • Searches for particulate methane monooxygenase (pmoA) and methyl-coenzyme M reductase (mcrA), marker genes for aerobic and anaerobic oxidation of methane respectively, identified pmoA in the 0-4 cm metagenome as Methylococcaceae related. (uio.no)
  • A metagenomic insight into freshwater methane-utilizing communities and evidence for cooperation between the Methylococcaceae and the Methylophilaceae. (uni-bielefeld.de)
  • Moreover, a decrease in the relative abundance of methanotrophs within the bacterial family Methylococcaceae was detected, concurrent with an increase in methane concentration in the water column. (asm.org)
  • Methanotrophs in Gammaproteobacteria are known from family Methylococcaceae. (wikipedia.org)
  • BOWMAN (J.P.), SLY (L.I.), NICHOLS (P.D.) and HAYWARD (A.C.): Revised taxonomy of the methanotrophs: description of Methylobacter gen. nov., emendation of Methylococcus , validation of Methylosinus and Methylocystis species, and a proposal that the family Methylococcaceae includes only the group I methanotrophs. (bacterio.net)
  • Bowman J (2006) The methanotrophs - the families Methylococcaceae and Methylocystaceae . (springer.com)
  • Revised taxonomy of the methanotrophs: Description of Methylobacter gen. nov., emendation of Methylococcus, validation of Methylosinus and Methylocystis species, and aproposal that the family Methylococcaceae includes only the Group I methanotrophs. (atcc.org)
  • Fatty acids characteristic of methanotrophs were shown to be abundant and 13 C-enriched in SIP samples, and DNA-SIP identified members of the methanotrophic family Methylococcaceae as major 13 CH 4 consumers. (osti.gov)
  • Gypsum-stimulated OTUs were affiliated with several potential sulfate-reducing (Syntrophobacter, Desulfovibrio, unclassified Desulfobulbaceae, unclassified Desulfobacteraceae) and sulfur-oxidizing taxa (Thiobacillus, unclassified Rhodocyclaceae), while gypsum-repressed OTUs were dominated by aerobic methanotrophs (Methylococcaceae). (uni-konstanz.de)
  • Methylophilaceae and Methylococcaceae) and aerobic heterotrophs (Sphingomonadaceae and Comamonadaceae), yet differed by specific core populations and lower diversity and evenness. (edu.sa)
  • The relative abundances of 16S rRNA and mRNA assigned to the methanotrophic Methylococcaceae , primarily Methylobacter , increased with depth. (asm.org)
  • The phylogenetic position of the family Methylococcaceae. (atcc.org)
  • Some species of the Methylococcaceae have formed with certain marine mussels endosymbiotic relationships. (wikipedia.org)