A species of METHYLOBACTERIUM which can utilize acetate, ethanol, or methylamine as a sole carbon source. (From Bergey's Manual of Determinative Bacteriology, 9th ed)
A genus of gram-negative, aerobic, facultatively methylotrophic rods occurring singly or occasionally in rosettes. Members of this genus are usually motile and are isolated from soil, dust, fresh water, lake sediments, leaf surfaces, rice, air, and hospital environments. (From Bergey's Manual of Determinative Bacteriology, 9th ed)
An enzyme that catalyzes the conversion of L-glutamate and water to 2-oxoglutarate and NH3 in the presence of NAD+. (From Enzyme Nomenclature, 1992) EC 1.4.1.2.
A large group of aerobic bacteria which show up as pink (negative) when treated by the gram-staining method. This is because the cell walls of gram-negative bacteria are low in peptidoglycan and thus have low affinity for violet stain and high affinity for the pink dye safranine.
A colorless, flammable liquid used in the manufacture of FORMALDEHYDE and ACETIC ACID, in chemical synthesis, antifreeze, and as a solvent. Ingestion of methanol is toxic and may cause blindness.
Compounds based on 2-amino-4-hydroxypteridine.
The simplest saturated hydrocarbon. It is a colorless, flammable gas, slightly soluble in water. It is one of the chief constituents of natural gas and is formed in the decomposition of organic matter. (Grant & Hackh's Chemical Dictionary, 5th ed)
A hydrocarbon used as an industrial solvent. It has been used as an aerosal propellent, as a refrigerant and as a local anesthetic. (From Martindale, The Extra Pharmacopoeia, 31st ed, p1403)
A sulfhydryl compound used to prevent urothelial toxicity by inactivating metabolites from ANTINEOPLASTIC AGENTS, such as IFOSFAMIDE or CYCLOPHOSPHAMIDE.
A genus of anaerobic, irregular spheroid-shaped METHANOSARCINALES whose organisms are nonmotile. Endospores are not formed. These archaea derive energy via formation of methane from acetate, methanol, mono-, di-, and trimethylamine, and possibly, carbon monoxide. Organisms are isolated from freshwater and marine environments.
A plant genus of the family LAMIACEAE that is a source of perilla alcohol and the oil is rich in alpha-linolenic acid (alpha-18:3).
A family of gram-negative, aerobic bacteria utilizing only one-carbon organic compounds and isolated from in soil and water.
A chlorinated hydrocarbon that has been used as an inhalation anesthetic and acts as a narcotic in high concentrations. Its primary use is as a solvent in manufacturing and food technology.
Messages between computer users via COMPUTER COMMUNICATION NETWORKS. This feature duplicates most of the features of paper mail, such as forwarding, multiple copies, and attachments of images and other file types, but with a speed advantage. The term also refers to an individual message sent in this way.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A group of cytochromes with covalent thioether linkages between either or both of the vinyl side chains of protoheme and the protein. (Enzyme Nomenclature, 1992, p539)
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
A publication issued at stated, more or less regular, intervals.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.

Halomethane:bisulfide/halide ion methyltransferase, an unusual corrinoid enzyme of environmental significance isolated from an aerobic methylotroph using chloromethane as the sole carbon source. (1/82)

A novel dehalogenating/transhalogenating enzyme, halomethane:bisulfide/halide ion methyltransferase, has been isolated from the facultatively methylotrophic bacterium strain CC495, which uses chloromethane (CH(3)Cl) as the sole carbon source. Purification of the enzyme to homogeneity was achieved in high yield by anion-exchange chromatography and gel filtration. The methyltransferase was composed of a 67-kDa protein with a corrinoid-bound cobalt atom. The purified enzyme was inactive but was activated by preincubation with 5 mM dithiothreitol and 0.5 mM CH(3)Cl; then it catalyzed methyl transfer from CH(3)Cl, CH(3)Br, or CH(3)I to the following acceptor ions (in order of decreasing efficacy): I(-), HS(-), Cl(-), Br(-), NO(2)(-), CN(-), and SCN(-). Spectral analysis indicated that cobalt in the native enzyme existed as cob(II)alamin, which upon activation was reduced to the cob(I)alamin state and then was oxidized to methyl cob(III)alamin. During catalysis, the enzyme shuttles between the methyl cob(III)alamin and cob(I)alamin states, being alternately demethylated by the acceptor ion and remethylated by halomethane. Mechanistically the methyltransferase shows features in common with cobalamin-dependent methionine synthase from Escherichia coli. However, the failure of specific inhibitors of methionine synthase such as propyl iodide, N(2)O, and Hg(2+) to affect the methyltransferase suggests significant differences. During CH(3)Cl degradation by strain CC495, the physiological acceptor ion for the enzyme is probably HS(-), a hypothesis supported by the detection in cell extracts of methanethiol oxidase and formaldehyde dehydrogenase activities which provide a metabolic route to formate. 16S rRNA sequence analysis indicated that strain CC495 clusters with Rhizobium spp. in the alpha subdivision of the Proteobacteria and is closely related to strain IMB-1, a recently isolated CH(3)Br-degrading bacterium (T. L. Connell Hancock, A. M. Costello, M. E. Lidstrom, and R. S. Oremland, Appl. Environ. Microbiol. 64:2899-2905, 1998). The presence of this methyltransferase in bacterial populations in soil and sediments, if widespread, has important environmental implications.  (+info)

Molecular analyses of novel methanotrophic communities in forest soil that oxidize atmospheric methane. (2/82)

Forest and other upland soils are important sinks for atmospheric CH(4), consuming 20 to 60 Tg of CH(4) per year. Consumption of atmospheric CH(4) by soil is a microbiological process. However, little is known about the methanotrophic bacterial community in forest soils. We measured vertical profiles of atmospheric CH(4) oxidation rates in a German forest soil and characterized the methanotrophic populations by PCR and denaturing gradient gel electrophoresis (DGGE) with primer sets targeting the pmoA gene, coding for the alpha subunit of the particulate methane monooxygenase, and the small-subunit rRNA gene (SSU rDNA) of all life. The forest soil was a sink for atmospheric CH(4) in situ and in vitro at all times. In winter, atmospheric CH(4) was oxidized in a well-defined subsurface soil layer (6 to 14 cm deep), whereas in summer, the complete soil core was active (0 cm to 26 cm deep). The content of total extractable DNA was about 10-fold higher in summer than in winter. It decreased with soil depth (0 to 28 cm deep) from about 40 to 1 microg DNA per g (dry weight) of soil. The PCR product concentration of SSU rDNA of all life was constant both in winter and in summer. However, the PCR product concentration of pmoA changed with depth and season. pmoA was detected only in soil layers with active CH(4) oxidation, i.e., 6 to 16 cm deep in winter and throughout the soil core in summer. The same methanotrophic populations were present in winter and summer. Layers with high CH(4) consumption rates also exhibited more bands of pmoA in DGGE, indicating that high CH(4) oxidation activity was positively correlated with the number of methanotrophic populations present. The pmoA sequences derived from excised DGGE bands were only distantly related to those of known methanotrophs, indicating the existence of unknown methanotrophs involved in atmospheric CH(4) consumption.  (+info)

Methylobacterium mesophilicum infection: case report and literature review of an unusual opportunistic pathogen. (3/82)

Methylobacterium mesophilicum is a methylotrophic, pink pigmented, gram-negative rod that was initially isolated from environmental sources that is being increasingly reported as a cause of opportunistic infections in immunocompromised hosts. We present the case of an immunocompromised woman who developed a central catheter infection with M. mesophilicum and review the other 29 cases reported in the literature, noting that it is frequently resistant to beta-lactam agents but is generally susceptible to aminoglycosides and quinolones.  (+info)

DNA polymerase I is essential for growth of Methylobacterium dichloromethanicum DM4 with dichloromethane. (4/82)

Methylobacterium dichloromethanicum DM4 grows with dichloromethane as the unique carbon and energy source by virtue of a single enzyme, dichloromethane dehalogenase-glutathione S-transferase. A mutant of the dichloromethane-degrading strain M. dichloromethanicum DM4, strain DM4-1445, was obtained by mini-Tn5 transposon mutagenesis that was no longer able to grow with dichloromethane. Dichloromethane dehalogenase activity in this mutant was comparable to that of the wild-type strain. The site of mini-Tn5 insertion in this mutant was located in the polA gene encoding DNA polymerase I, an enzyme with a well-known role in DNA repair. DNA polymerase activity was not detected in cell extracts of the polA mutant. Conjugation of a plasmid containing the intact DNA polymerase I gene into the polA mutant restored growth with dichloromethane, indicating that the polA gene defect was responsible for the observed lack of growth of this mutant with dichloromethane. Viability of the DM4-1445 mutant was strongly reduced upon exposure to both UV light and dichloromethane. The polA'-lacZ transcriptional fusion resulting from mini-Tn5 insertion was constitutively expressed at high levels and induced about twofold after addition of 10 mM dichloromethane. Taken together, these data indicate that DNA polymerase I is essential for growth of M. dichloromethanicum DM4 with dichloromethane and further suggest an important role of the DNA repair machinery in the degradation of halogenated, DNA-alkylating compounds by bacteria.  (+info)

Methylotrophic Methylobacterium bacteria nodulate and fix nitrogen in symbiosis with legumes. (5/82)

Rhizobia described so far belong to three distinct phylogenetic branches within the alpha-2 subclass of Proteobacteria. Here we report the discovery of a fourth rhizobial branch involving bacteria of the Methylobacterium genus. Rhizobia isolated from Crotalaria legumes were assigned to a new species, "Methylobacterium nodulans," within the Methylobacterium genus on the basis of 16S ribosomal DNA analyses. We demonstrated that these rhizobia facultatively grow on methanol, which is a characteristic of Methylobacterium spp. but a unique feature among rhizobia. Genes encoding two key enzymes of methylotrophy and nodulation, the mxaF gene, encoding the alpha subunit of the methanol dehydrogenase, and the nodA gene, encoding an acyltransferase involved in Nod factor biosynthesis, were sequenced for the type strain, ORS2060. Plant tests and nodA amplification assays showed that "M. nodulans" is the only nodulating Methylobacterium sp. identified so far. Phylogenetic sequence analysis showed that "M. nodulans" NodA is closely related to Bradyrhizobium NodA, suggesting that this gene was acquired by horizontal gene transfer.  (+info)

Hyphomicrobium chloromethanicum sp. nov. and Methylobacterium chloromethanicum sp. nov., chloromethane-utilizing bacteria isolated from a polluted environment. (6/82)

Two chloromethane-utilizing facultatively methylotrophic bacteria, strains CM2T and CM4T, were isolated from soil at a petrochemical factory. On the basis of their morphological, physiological and genotypical properties, strain CM2T (= VKM B-2176T = NCIMB 13687T) is proposed as a new species of the genus Hyphomicrobium, Hyphomicrobium chloromethanicum, and strain CM4T (= VKM B-2223T = NCIMB 13688T) as a new species of the genus Methylobacterium, Methylobacterium chloromethanicum.  (+info)

Sequence variation in dichloromethane dehalogenases/glutathione S-transferases. (7/82)

Dichloromethane dehalogenase/glutathione S-transferase allows methylotrophic bacteria to grow with dichloromethane (DCM), a predominantly man-made compound. Bacteria growing with DCM by virtue of this enzyme have been readily isolated in the past. So far, the sequence of the dcmA gene encoding DCM dehalogenase has been determined for Methylobacterium dichloromethanicum DM4 and Methylophilus sp. DM11. DCM dehalogenase genes closely related to that of strain DM4 were amplified by PCR and cloned from total DNA from 14 different DCM-degrading strains, enrichment cultures and sludge samples from wastewater treatment plants. In total, eight different sequences encoding seven different protein sequences were obtained. Sequences of different origin were identical in several instances. Sequence variation was limited to base substitutions; strikingly, 16 of the 19 substitutions in the dcmA gene itself encoded amino acids that were different from those of the DM4 sequence. The kinetic parameters k(cat) and K:(m), the pH optimum and the stability of representative DCM dehalogenase variants were investigated, revealing minor differences between the properties of DCM dehalogenases related to that from strain DM4.  (+info)

Chloromethane: tetrahydrofolate methyl transfer by two proteins from Methylobacterium chloromethanicum strain CM4. (8/82)

The cmuA and cmuB genes are required for growth of Methylobacterium chloromethanicum strain CM4 with chloromethane as the sole carbon source. While CmuB was previously shown to possess methylcobalamin:tetrahydrofolate methyltransferase activity, sequence analysis indicated that CmuA represented a novel and so far unique two-domain methyltransferase/corrinoid-binding protein involved in methyl transfer from chloromethane to a corrin moiety. CmuA was purified from wild-type M. chloromethanicum strain CM4 and characterized as a monomeric, cobalt-containing and zinc-containing enzyme of molecular mass 67 kDa with a bound vitamin B12 cofactor. In combination, CmuA and CmuB proteins catalyze the in vitro transfer of the methyl group of chloromethane to tetrahydrofolate, thus affording a direct link between chloromethane dehalogenation and core C1 metabolism of Methylobacterium. Chloromethane dehalogenase activity in vitro is limited by CmuB, as formation of methyltetrahydrofolate from chloromethane displays apparent Michaelis-Menten kinetics with respect to methylated CmuA, with an apparent Km of 0.27 microM and a Vmax of 0.45 U x mg(-1). This contrasts with sequence-related systems for methyl transfer from methanogens, which involve methyltransferase and corrinoid protein components in well-defined stoichiometric ratios.  (+info)

Methylobacterium radiotolerans ATCC ® 27329D-5™ Designation: Genomic DNA from Methylobacterium radiotolerans strain O-1 TypeStrain=True Application:
Fingerprint Dive into the research topics of Lanthanide-dependent methanol and formaldehyde oxidation in methylobacterium aquaticum strain 22A. Together they form a unique fingerprint. ...
Original data comes from a project which takes or took place as part of the DFG priority program Exploratories for large-scale and long-term functional biodiversity research. The data is stored together with descriptive metadata, in combination called a dataset, in the project repository (https://www.bexis.uni-jena.de). Species information was extracted from that original dataset. The second paragraph is part of the metadata of the original dataset.Pink-pigmented colonies with different morphologies were isolated to asses Methylobacterium community composition on leaf surfaces with cultivation-dependent methods ...
In examining the presence of indole-3-acetic acid (IAA) in supernatants of pink-pigmented facultativemethylotrophic (PPFMs) bacterial cultures, three out of the 16 isolates tested showed a positive re
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ID B8I9H1_METNO Unreviewed; 254 AA. AC B8I9H1; DT 03-MAR-2009, integrated into UniProtKB/TrEMBL. DT 03-MAR-2009, sequence version 1. DT 08-MAY-2019, entry version 53. DE RecName: Full=Heme exporter protein C {ECO:0000256,RuleBase:RU364092}; DE AltName: Full=Cytochrome c-type biogenesis protein {ECO:0000256,RuleBase:RU364092}; GN Name=ccmC {ECO:0000256,RuleBase:RU364092}; GN OrderedLocusNames=Mnod_0178 {ECO:0000313,EMBL:ACL55224.1}; OS Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060). OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; OC Methylobacteriaceae; Methylobacterium. OX NCBI_TaxID=460265 {ECO:0000313,EMBL:ACL55224.1, ECO:0000313,Proteomes:UP000008207}; RN [1] {ECO:0000313,EMBL:ACL55224.1, ECO:0000313,Proteomes:UP000008207} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=LMG 21967 / CNCM I-2342 / ORS 2060 RC {ECO:0000313,Proteomes:UP000008207}; RG US DOE Joint Genome Institute; RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., ...
ID B8I9Q2_METNO Unreviewed; 542 AA. AC B8I9Q2; DT 03-MAR-2009, integrated into UniProtKB/TrEMBL. DT 03-MAR-2009, sequence version 1. DT 05-JUL-2017, entry version 63. DE RecName: Full=Cytochrome c oxidase subunit 1 {ECO:0000256,RuleBase:RU363061}; DE EC=1.9.3.1 {ECO:0000256,RuleBase:RU363061}; GN OrderedLocusNames=Mnod_0261 {ECO:0000313,EMBL:ACL55305.1}; OS Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060). OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; OC Methylobacteriaceae; Methylobacterium. OX NCBI_TaxID=460265 {ECO:0000313,EMBL:ACL55305.1, ECO:0000313,Proteomes:UP000008207}; RN [1] {ECO:0000313,EMBL:ACL55305.1, ECO:0000313,Proteomes:UP000008207} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=LMG 21967 / CNCM I-2342 / ORS 2060 RC {ECO:0000313,Proteomes:UP000008207}; RG US DOE Joint Genome Institute; RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., RA Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., RA Detter ...
Study compares bacterial composition in healthy vs. cancerous breast tissue. Source:. Cleveland Clinic. Summary:. Researchers have uncovered differences in the bacterial composition of breast tissue of healthy women vs. women with breast cancer. The research team has discovered for the first time that healthy breast tissue contains more of the bacterial species Methylobacterium, a finding which could offer a new perspective in the battle against breast cancer.. FULL STORY. Bacteria that live in the body, known as the microbiome, influence many diseases.. In a newly published study, Cleveland Clinic researchers have uncovered differences in the bacterial composition of breast tissue of healthy women vs. women with breast cancer. The research team has discovered for the first time that healthy breast tissue contains more of the bacterial species Methylobacterium, a finding which could offer a new perspective in the battle against breast cancer.. Bacteria that live in the body, known as the ...
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p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
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Methylobacterium rhodinum 16S ribosomal RNA gene, partial sequence;tRNA-Ile and tRNA-Ala genes, complete sequence; and 23S ribosomal RNA gene,partial ...
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The objective in the present thesis was to evaluate possible improvements when using training as a treatment for peripheral arterial disease (PAD) patients and to study possible mechanisms behind the adaptations to training. During exercise patients with PAD are limited by muscle pain in the lower extremities, distal to the atherosclerotic lesions, due to lack of oxygen. In the first experiment high aerobic intensity whole body treadmill endurance training was found to be superior to previous recommendations using moderate aerobic intensity. During the 8 weeks of training whole body peak oxygen consumption (VO2peak) and time to exhaustion when walking improved, whereas no changes in blood flow were observed. Since the high aerobic intensity treadmill walking showed muscular, but not cardiac output or stroke volume responses, it was hypothesized in paper II that specific calf muscle individual leg training could result in even better effect on the distal limitations from the PAD. The calf muscle ...
A pink-pigmented, facultatively methylotrophic bacterium, strain 23eT, was isolated from the leaves of Gnaphalium spicatum (cudweed). The cells of strain 23eT were Gram-reaction negative, motile and non-spore-forming rods. On the basis of 16S rRNA gene sequence similarities, strain 23eT was related to Methylobacterium organophilum ATCC 27886T (97.1 %) and Methylobacterium marchantiae JT1T (97 %), and the phylogenetic similarities to all other Methylobacterium species with validly published names were less than 97 %. Major cellular fatty acids were C18 : 1ω7c, C16 : 00 and C18 : 0. The results of DNA-DNA hybridization, phylogenetic analyses based on 16S rRNA and cpn60 gene sequences, fatty acid profiles, whole-cell matrix-assisted laser desorption/ionization time of flight/MS analysis, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 23eT from the phylogenetically closest relatives. We propose that strain 23eT represents a novel species within the genus
16S ribosomal RNAs (rRNA) of 12 methylotrophic bacteria have been almost completely sequenced to establish their phylogenetic relationships. Methylotrophs that are physiologically related are phylogenetically diverse and are scattered among the purple eubacteria (class Proteobacteria). Group I methylotrophs can be classified in the β-and the γ-subdivisions and group II methylotrophs in the α-subdivision of the purple eubacteria, respectively. Pink-pigmented facultative and non-pigmented obligate group II methylotrophs form two distinctly separate branches within the α-subdivision. The secondary structures of the 16S rRNA sequences of Methylocystis parvus strain OBBP, Methylosinus trichosporium strain OB3b, Methylosporovibrio methanica strain 81Z and Hyphomicrobium sp. strain DM2 are similar, and these non-pigmented obligate group II methylotrophs form one tight cluster in the α-subdivision. The pink-pigmented facultative methylotrophs, Methylobacterium extorquens strain AM1, Methylobacterium
Volatile organic compounds from Penicillium viridicatum and Methylobacterium mesophilicum growing on laboratory media and on component materials of automobile air conditioners were analyzed with gas chromatography and mass spectrometry. P. viridicatum produced compounds such as 4-methyl thiazole, te …
The discovery of several members of the Leguminosae family growing on a TCE contaminated site demonstrated that they have the capabilities to tolerate the TCE contamination. This led to an investigation of the plants ability to metabolize TCE. Research by Dr. Lee Newman showed that nodules from Crotalaria spectabilis grown on the site had TCE metabolites present while nodules from other legumes at the site did not. The presence of TCE metabolites within the C. spectabilis nodules suggests that the bacterial symbiont may be responsible for TCE metabolism. This led us to investigate the symbiont of C. spectabilis and its ability to metabolize TCE. Nodules from C. spectabilis grown on TCE contaminated and non-contaminated soils were used to isolate the bacterial symbiont. Some species of Crotalaria are known to host Methylobacterium nodulans and this unique symbiont may explain why only the C. spectabilis nodules metabolize TCE. However, methane and methanol enrichments from the nodules were not
Methylotrophy describes the ability of organisms to grow on reduced organic compounds without carbon-carbon bonds. Methylobacterium extorquens AM1 is capable of growth on one-carbon compounds such as methanol. Methanol is oxidized to formaldehyde which is then used metabolically to generate either energy or biomass. These bacteria are commonly found in the environment, especially associated with plants which produce methanol when metabolizing pectin during cell wall synthesis. The 6.88 Mb genome of strain AM1 comprises a 5.51 Mb chromosome, a 1.26 Mb megaplasmid and three plasmids. [Vuilleumier 2009]. ...
Methylobacterium extorquens AM1 has been considered as a potential platform strain for industrial production of valuable chemicals such as mevalonate, 1-butanol and 2-hydroxyisobutyrate [30, 32, 33, 41]. In this work, we first optimized a 3-HP synthetic pathway in M. extorquens AM1, and then focused on the demonstration of the mechanism of 3-HP reassimilation.. It has been reported that tuning of gene expression levels was critical for proper functioning of a heterologous synthetic pathway in M. extorquens AM1. For instance, Hu et al. found that the strain expressing the adhE2 and ter from a promoter of intermediate strength produced the highest 1-butanol [32]. In our case, four different promoter strengths were tested and the strongest promoter mxaF was shown to generate the highest 3-HP, comparable with the preliminary titer of other engineered microorganisms [5]. The pool size of precursor acetyl-CoA was similar between the YHP5 strains and the other three recombinant strains, implying that ...
Methylobacterium extorquens ATCC ® 14718™ Designation: NCIB 9133 TypeStrain=False Application: Produces glutamate dehydrogenase (NADP+) NADP+-dependent glutamate dehydrogenase, glutamate dehydrogenase (NADP-dependent) Biotechnology
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Roseomonas is a genus of Gram negative bacteria. The cells are coccoid rods when viewed microscopically. Certain species are known to be opportunistic infections for humans. The first species isolated had pink colonies when grown on agar, which led to the naming of the genus (from Latin roseus, pink). Roseomonas species have been isolated frequently from blood, wounds, exudates, abscesses and genitourinary specimens, and have been isolated from environmental sources. Roseomonas can cause bacteremia in humans, especially in immunocompromised patients. While uncommon, the reported rate of Roseomonas infection may be lower due to difficulty in detection of the bacteria in clinical settings. The first species of Roseomonas were identified when 42 strains of pink-pigmented bacteria from various clinical sources were examined. While similar to characteristics of genus Methylobacterium, the strains were found to contain three new species of a previously unidentified genus, which led to the creation ...
There is evidence that methanol is consumed by Methylobacterium and contributes to the epiphytic fitness of the organism. However, in addition to the peak of methanol emission in the morning, Methylobacterium should adapt its metabolism to use additional carbon sources during the rest of the day when methanol emission is low or during the night when stomata are closed and methanol is consequently no longer available. PPFMs are aerobic, Gram-negative bacteria and, although they are able to grow on a wide range of multicarbon substrates, they are characterized by the capability to grow on one-carbon compounds such as formate, formaldehyde, and methanol as their sole carbon and energy source. They are easily isolated on a methanol based mineral medium. Recently published data suggest that the degree of the plant-Methylobacterium association varies from strong, or symbiotic to loose, or epiphytic, a range that also includes the intermediate endophytic association. However, the overall nature of ...
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Author: Schwachtje, J. et al.; Genre: Journal Article; Published in Print: 2012; Keywords: Arabidopsis/*growth & development/*microbiology|br/|Biological Assay|br/|Carbon-Carbon Lyases/metabolism|br/|*Ecotype|br/|Indoleacetic Acids/metabolism|br/|Phosphates/metabolism|br/|Rhizobium/enzymology/*isolation & purification|br/|Solubility; Title: Plant-growth promoting effect of newly isolated rhizobacteria varies between two Arabidopsis ecotypes
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ID C5ARV5_METEA Unreviewed; 396 AA. AC C5ARV5; DT 28-JUL-2009, integrated into UniProtKB/TrEMBL. DT 28-JUL-2009, sequence version 1. DT 22-NOV-2017, entry version 63. DE RecName: Full=Elongation factor Tu {ECO:0000256,HAMAP-Rule:MF_00118, ECO:0000256,RuleBase:RU004061}; DE Short=EF-Tu {ECO:0000256,HAMAP-Rule:MF_00118}; GN Name=tuf {ECO:0000256,HAMAP-Rule:MF_00118, GN ECO:0000313,EMBL:ACS42443.1}; GN OrderedLocusNames=MexAM1_META1p4832 {ECO:0000313,EMBL:ACS42443.1}; OS Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / OS NCIMB 9133 / AM1). OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; OC Methylobacteriaceae; Methylobacterium. OX NCBI_TaxID=272630 {ECO:0000313,EMBL:ACS42443.1, ECO:0000313,Proteomes:UP000009081}; RN [1] {ECO:0000313,EMBL:ACS42443.1, ECO:0000313,Proteomes:UP000009081} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 RC {ECO:0000313,Proteomes:UP000009081}; RX PubMed=19440302; ...
Please cite Turkarslan et al., 2017 Mol. Sys. Biol. if you find Syntrophy Portal useful.. Acknowledgement: This material by ENIGMA- Ecosystems and Networks Integrated with Genes and Molecular Assemblies (http://enigma.lbl.gov), a Scientific Focus Area Program at Lawrence Berkeley National Laboratory is based upon work supported by the U.S. Department of Energy, Office of Science, Office of Biological & Environmental Research under contract number DE-AC02-05CH11231 ...
Please cite Turkarslan et al., 2017 Mol. Sys. Biol. if you find Syntrophy Portal useful.. Acknowledgement: This material by ENIGMA- Ecosystems and Networks Integrated with Genes and Molecular Assemblies (http://enigma.lbl.gov), a Scientific Focus Area Program at Lawrence Berkeley National Laboratory is based upon work supported by the U.S. Department of Energy, Office of Science, Office of Biological & Environmental Research under contract number DE-AC02-05CH11231 ...
Bulman, Zack, Andre O. Hudson, and Michael A. Savka. Propolis, a Product of the Bee Hive, has an Antagonistic Affect on Quorum-sensing Regulated Bioluminescence, Gene Transcription and Motility. Proceedings of the American Society for Biochemistry & Molecular Biology Meeting. Ed. K. Cornely, P. Ortiz, and M.A. Wallert. Washington, DC: American Society for Biochemistry & Molecular Biology, 2011. Print. *. Subki, Mior MM Ahmad, et al. Isolation and Identification of Methylobacterium sp. From Grapevine Xylem Fluids and Mutants Deficient in Acyl-homoserine Signal Production, Abstract 818. Proceedings of the American Society for Microbiology, 111th General Meeting. Ed. American Society for Microbiology. New Orleans, LA: American Society for Microbiology, 2011. Print. *. Aziz, Nazrin A. and Michael A. Savka. Screening of Transposon Mutants of Sphingobium sp. KK22 Altered in Quorum-sensing Signal Synthesis. Proceedings of the Rochester Academy of Sciences, Fall 2011 Scientific Paper Day. Ed. ...
Eww … theres weird pink stuff growing in the bathroom. Gross. If you have pink stains in your shower and pinkish sludge on your shower curtain, then youre probably looking at a case of pink mold. What is this common bathroom infestation and how do you get rid of it?What pink mold really isPink mold usually appears as a fuzzy (but not in a good way) or slimy buildup in damp areas of your home - most commonly on the shower curtain or the grout between
Eww … theres weird pink stuff growing in the bathroom. Gross. If you have pink stains in your shower and pinkish sludge on your shower curtain, then youre probably looking at a case of pink mold. What is this common bathroom infestation and how do you get rid of it?What pink mold really isPink mold usually appears as a fuzzy (but not in a good way) or slimy buildup in damp areas of your home - most commonly on the shower curtain or the grout between
Eww … theres weird pink stuff growing in the bathroom. Gross. If you have pink stains in your shower and pinkish sludge on your shower curtain, then youre probably looking at a case of pink mold. What is this common bathroom infestation and how do you get rid of it?What pink mold really isPink mold usually appears as a fuzzy (but not in a good way) or slimy buildup in damp areas of your home - most commonly on the shower curtain or the grout between
Eww … theres weird pink stuff growing in the bathroom. Gross. If you have pink stains in your shower and pinkish sludge on your shower curtain, then youre probably looking at a case of pink mold. What is this common bathroom infestation and how do you get rid of it?What pink mold really isPink mold usually appears as a fuzzy (but not in a good way) or slimy buildup in damp areas of your home - most commonly on the shower curtain or the grout between
Eww … theres weird pink stuff growing in the bathroom. Gross. If you have pink stains in your shower and pinkish sludge on your shower curtain, then youre probably looking at a case of pink mold. What is this common bathroom infestation and how do you get rid of it?What pink mold really isPink mold usually appears as a fuzzy (but not in a good way) or slimy buildup in damp areas of your home - most commonly on the shower curtain or the grout between
Eww … theres weird pink stuff growing in the bathroom. Gross. If you have pink stains in your shower and pinkish sludge on your shower curtain, then youre probably looking at a case of pink mold. What is this common bathroom infestation and how do you get rid of it?What pink mold really isPink mold usually appears as a fuzzy (but not in a good way) or slimy buildup in damp areas of your home - most commonly on the shower curtain or the grout between
That is one among the primary makes use of of using a corticosteroid like prednisone. MyBroadband I can say a hundred% that it really works and in 90 days Ive nearly all my hair I used propecia (need prescription) and minoxidil which youll get Finasteride vs. To start with, the web has introduced an incredible market opportunity for every one of the travel and leisure organizations. The generic finasteride needs no marketing as well as analysis because these are accomplished by the model company. The producer could have changed the elements. You shouldnt have any worry because whether you are taking this Propecia drug within the model variant or generic kind, the effectiveness in your body is identical. There are such a lot of to select from, however your vet solely ever seems to offer one or two. The doctor could prescribe finasteride as an alternative, and theres really no purpose not to decide on the generic version, normally.. Are there any supplements I should keep away from when taking ...
The draft genome of S. proteamaculans S4 was generated using a combination of Illumina and 454 sequencing platforms. The details of library construction and sequencing are available at the JGI [26]. The sequence data generated from Illumina GAii (4,232 Mb) were assembled with Velvet [27] and the consensus sequence was computationally shredded into 1.5 kb overlapping fake reads. The sequencing data generated from 454 pyrosequencing (89.5 Mb) were assembled with Newbler and consensus sequences were computationally shredded into 2 kb overlapping fake reads. The initial draft assembly contained 50 contigs in 2 scaffolds. The 454 Newbler consensus reads, the Illumina Velvet consensus reads and the read pairs in the 454 paired end library were integrated using parallel Phrap [28,29]. The software, Consed [30] was used for the subsequent finishing process. The software Polisher [31] was used to correct the base errors and increase the consensus quality. Possible mis-assemblies were corrected with ...
Post-doctoral fellow, 2010-2014, Harvard University. Research Interests. Please see my website.. Selected Publications. Chubiz, L.M., Granger, B.R., Segrè, D., Harcombe, W.R., Species interactions differ in their genetic robustness, Frontiers Microbiol., (2015). Chubiz, L.M., Purswani, J., Carroll, S.M. and Marx, C.J., A novel pair of inducible expression vectors for use in Methylobacterium extorquens, BMC Research Notes, 6(186) (2013). Chubiz, L.M., Lee, M.C., Delaney, N.F. and Marx, C.J., FREQ-Seq: a rapid, cost-effective, sequencingbased method to determine allele frequencies directly from mixed populations. PLoS One, 7(10)(2012). Faculty of 1000 recommended by Drs. Andreas Wagner and Kathleen Sprouffske. Chubiz, L.M.,, Glekas, G.D. and Rao, C.V., Transcriptional cross talk within the mar-sox-rob regulon in Escherichia coli is limited to the rob and marRAB operons, Journal of Bacteriology, 194(18):4867-4875 (2012). Chubiz, L.M. and Rao, C.V., Role of the mar-sox-rob regulon in ...
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Dedication. This chapter is dedicated to The Cabanas Guesthouse and Spa (www.thecabanasguesthouse.com) in Wilton Manors, FL. (thats Fort Lauderdale, folks). The accommodations and staff there are first rate and carry my highest recommendation. However, as I discovered, it is a very popular place so make reservations early.. To answer a challenge from my friend (and editor) Mike, I was finally able to come up with two things I didnt like... The shower curtains blow in and touched my leg and the communal ice dispenser does not work fast enough. Minor inconveniences both.. The Usual Stuff. Thanks as always go to my friend Mike who continues to proofread and edit for me, not to mention offering many helpful suggestions. That assistance is essential to the quality of what you are reading.. Thanks also to the Nifty Archive for hosting this and all the other stories. They do take donations to defray operating expenses, so please help them out as much as you can.. This story contains graphic ...
Today was voting day here in California. We had a couple state propositions to decide on, plus local measures, and local elections. I got up, voted, then caught the bus to BART to go into the City. I checked my mail and closed my savings account (the bank was too far away and too far out). I only had about $69 in the account anyway. My exercise was walking to work from where I get off BART. I stopped at WalMart and spent most of the money Id just got from savings. I mostly bought food items, but I did get a shower curtain since my current one is pretty much ready for the trash and I want to leave a new one behind me when I move so the owners dont charge me for that). I checked the price of venetian blinds and they look cheap enough. I have to replace at least two that the cats ruined ...
There was more story, but less fun on Life Unexpected. After two really good episodes in a row, this one was a little blah. Lux continued to flirt with Tiny Teacher and also be a raving bitch to him. He continued to like her inappropriately and resist getting completely out of her life. He forces tutoring on her while also sleeping with Paige in the loft where Lux is staying and where the walls are made of shower curtains. Not only is that inappropriate, but its also pretty gross and deeply awkward for them and the viewers. He tells her that he HAS to keep tutoring her because thats the only way its appropriate for them to be together and for him to lust after her. Um...no! He figures out that she has a learning disability then promptly dumps Paige and arranges for someone else to tutor Lux. After last week when I was all relieved that someone finally squashed a student/teacher affair before it got out of control, and then this week it was once again un-squashed, Im not falling for it again. ...
Experimental Animal Center, Chinese Academy of Sciences and raised under SPF conditions. Those of 8 weeks old, weighing 20-22 g were used for bacterial inoculation.. ...
Green PN (2001). "Methylobacterium". In Dworkin M (ed.). The Prokaryotes: An Evolving Electronic Resource for the ... Koenig RL, Morris RO, Polacco JC (April 2002). "tRNA is the source of low-level trans-zeatin production in Methylobacterium spp ... Siddikee M, Hamayun M, Han GH, Sa TM (2010). "Optimization of gibberellic acid production by Methylobacterium oryzae CBMB20". ... by Methylobacterium spp. Botanical". Bulletin of Academia Sinica: 45. Doronina NV, Ivanova EG, Trotsenko I (2002). "[New ...
O'Connor M, Wopat A, Hanson RS (1977). "Genetic transformation in Methylobacterium organophilum". J. Gen. Microbiol. 98 (1): ... Methylobacterium spp.), symbionts of plants (e.g., Rhizobium spp.), endosymbionts of arthropods (Wolbachia) and intracellular ... Methylobacterium organophilum, and Bradyrhizobium japonicum. Natural genetic transformation is a sexual process involving DNA ...
"Methylobacterium cerastii sp. nov., isolated from the leaf surface of Cerastium holosteoides". International Journal of ...
Scientists report the discovery of unknown species of bacteria of Methylobacterium, tentatively named Methylobacterium ajmalii ... "Methylobacterium ajmalii sp. nov., Isolated From the International Space Station". Frontiers in Microbiology. 12. doi:10.3389/ ...
15 March - Scientists report the discovery of a new unknown bacteria species, for the first time, of Methylobacterium, ... tentatively named Methylobacterium ajmalii, associated with three new strains, designated IF7SW-B2T, IIF1SW-B5, and IIF4SW-B5, ... "Methylobacterium ajmalii sp. nov., Isolated From the International Space Station". Frontiers in Microbiology. 12. doi:10.3389/ ...
"Methylobacterium ajmalii sp. nov., Isolated From the International Space Station". Frontiers in Microbiology. 12. doi:10.3389/ ...
"Methylobacterium cerastii sp. nov., a novel species isolated from the leaf surface of Cerastium holosteoides". International ...
O'Connor M, Wopat A, Hanson RS (1977). "Genetic transformation in Methylobacterium organophilum". J. Gen. Microbiol. 98 (1): ... Methylobacterium organophilum, and Bradyrhizobium japonicum. Natural genetic transformation is a sexual process involving DNA ...
and Methylobacterium chloromethanicum sp. nov., chloromethane-utilizing bacteria isolated from a polluted environment". Int J ...
Sahin, N; Kato, Y; Yilmaz, F (October 2008). "Taxonomy of oxalotrophic Methylobacterium strains". Die Naturwissenschaften. 95 ( ...
and Methylobacterium dichloromethanicum sp. nov.--novel aerobic facultatively methylotrophic bacteria utilizing dichloromethane ... and Methylobacterium dichloromethanicum sp. nov.--novel aerobic facultatively methylotrophic bacteria utilizing dichloromethane ...
This protein family is found in Caulobacter and Methylobacterium. Crescentin was recently discovered by Christine Jacobs-Wagner ...
"The atomic resolution structure of methanol dehydrogenase from Methylobacterium extorquens". Acta Crystallographica Section D. ...
"Formaldehyde-detoxifying role of the tetrahydromethanopterin-linked pathway in Methylobacterium extorquens AM1". J. Bacteriol. ...
In the methylotrophic bacterium Methylobacterium extorquens, however, it acts as a methenyltetrahydrofolate cyclohydrolase, ... "A methenyl tetrahydromethanopterin cyclohydrolase and a methenyl tetrahydrofolate cyclohydrolase in Methylobacterium extorquens ...
In the methylotrophic bacterium Methylobacterium extorquens, Ftr interacts with three other polypeptides to form an Ftr/ ... from Methylobacterium extorquens AM1". FEBS Lett. 523 (1-3): 133-7. doi:10.1016/S0014-5793(02)02962-9. PMID 12123819. S2CID ...
PHB is produced by microorganisms (such as Cupriavidus necator, Methylobacterium rhodesianum or Bacillus megaterium) apparently ... "Methylobacterium rhodesianum cells tend to double the DNA content under growth limitations and accumulate PHB". Journal of ...
"The refined structure of the quinoprotein methanol dehydrogenase from Methylobacterium extorquens at 1.94 A". Structure. 3 (2 ...
... of the quinoprotein alcohol dehydrogenase of Acetobacter aceti modelled on that of methanol dehydrogenase from Methylobacterium ...
Methylobacterium, Paracoccus, Pseudomonas Starkeya, Thermithiobacillus, and Xanthobacter. On the other hand, the cable bacteria ...
Chistoserdova, L. V.; Lidstrom, M. E. (1994-12-01). "Genetics of the serine cycle in Methylobacterium extorquens AM1: ... "Identification of a Fourth Formate Dehydrogenase in Methylobacterium extorquens AM1 and Confirmation of the Essential Role of ...
"Sequence analysis of pqq genes required for biosynthesis of pyrroloquinoline quinone in Methylobacterium extorquens AM1 and the ...
This family of proteins also includes amicyanin from bacteria such as Methylobacterium extorquens or Paracoccus versutus ( ...
During later studies of interactions between diplopterol and lipid A in Methylobacterium extorquens, multidrug transport was ...
Hemisynthesis of deuteriated adenosylhopane and conversion into bacteriohopanetetrol by a cell-free system of Methylobacterium ... reduction of ribosylhopane into bacteriohopanetetrol by a cell-free system from Methylobacterium organophilum, ChemBioChem 16, ...
Genus Methylobacterium. *Genus Microvirga. *Genus Protomonas. *Genus Roseomonas. *Famili Methylocystaceae *Genus Methylocystis ...
... because pink algae is not a true algae but is actually caused by a bacterium in the genus Methylobacterium. The color of the ...
... and Methylobacterium extorquens (alpha-proteobacteria) indicated that these species utilize the linear oxidation pathway via ... whereas Methylobacterium extorquens uses this pathway as its "main energy-generating pathway for methylotrophic growth." ... is more closely related to Methylobacterium extorquens (alphaproteobacteria) and Methylococcus capsulatus (gammaproteobacteria ...
While similar to characteristics of genus Methylobacterium, the strains were found to contain three new species of a previously ...
A rapid method for the purification of methanol dehydrogenase from Methylobacterium extorquens. Prot Exp Pur 46:316-320. ...
Parte, A.C. "Methylobacterium". LPSN. Madhaiyan, M; Poonguzhali, S (July 2014). "Methylobacterium pseudosasicola sp. nov. and ... Methylobacterium organophilum cells are able to undergo genetic transformation and become competent for DNA uptake near the end ... As well as its normal habitats in soil and water, Methylobacterium has also been identified as a contaminant of DNA extraction ... One species, found on a HEPA filter in the stations life-support system, was a garden-variety (literally!) Methylobacterium ...
Methylobacterium bullatum is a Gram-negative, facultatively methylotrophic, strictly aerobic and non-spore-forming bacteria ... Type strain of Methylobacterium bullatum at BacDive - the Bacterial Diversity Metadatabase v t e. ... Hoppe, T; Peters, K; Schmidt, F (November 2011). "Methylobacterium bullatum sp. nov., a methylotrophic bacterium isolated from ... LPSN lpsn.dsmz.de Straininfo of Methylobacterium bullatum UniProt Deutsche Sammlung von Mikroorganismen und Zellkulturen [1] ...
Methylobacterium populi BJ001, complete genome Methylobacterium populi BJ001, complete genome. gi,188579286,ref,NC_010725.1, ...
To take advantage of this technology, 60-mer oligonucleotide microarrays were designed for Methylobacterium extorquens AM1 to ... Implementation of microarrays for Methylobacterium extorquens AM1 OMICS. Winter 2007;11(4):325-40. doi: 10.1089/omi.2007.0027. ... To take advantage of this technology, 60-mer oligonucleotide microarrays were designed for Methylobacterium extorquens AM1 to ...
Methylobacterium extorquens ATCC ® 14718™ Designation: NCIB 9133 TypeStrain=False Application: Produces glutamate dehydrogenase ... Methylobacterium extorquens (Urakami and Komagata) Bousfield and Green (ATCC® 14718D-5™) Add to ... Methylobacterium extorquens (Urakami and Komagata) Bousfield and Green (ATCC® 14718™) Strain Designations: NCIB 9133 [D 355; ... Three new Methylobacterium species: M. rhodesianum sp. nov., M. zatmanii sp. nov., and M. fujisawaense sp. nov.. Int. J. Syst. ...
Methylobacterium extorquens ATCC ® 55366™ Designation: 58 TypeStrain=False Application: Produces poly-beta-hydroxybutyric acid ... Methylobacterium extorquens (Urakami and Komagata) Bousfield and Green ATCC® 55366™ frozen For-Profit: $354.00 Non-Profit: $ ... Methylobacterium extorquens (Urakami and Komagata) Bousfield and Green (ATCC® 55366™) Strain Designations: 58 / Type Strain: no ... Methylobacterium extorquwns microorganism useful for the preparation of poly-beta-hydroxybutyric acid polymers. US Patent ...
M00130 Inositol phosphate metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 ...
Methane metabolism - Methylobacterium extorquens CM4 [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ...
Asymmetric, bimodal trade-offs during adaptation of Methylobacterium to distinct growth substrates.. Lee MC1, Chou HH, Marx CJ. ... Here, we use adaptation of experimental populations of the model methylotroph, Methylobacterium extorquens AM1, to C(1) ( ... metabolism across the genus Methylobacterium requires relatively frequent use of C(1) compounds to prevent rapid loss. ...
Methylobacterium extorquens [TaxId: 408] B. d1h4jb_. All alpha proteins Non-globular all-alpha subunits of globular proteins ... Methylobacterium extorquens [TaxId: 408] D. d1h4jd_. All alpha proteins Non-globular all-alpha subunits of globular proteins ... Methylobacterium extorquens [TaxId: 408] F. d1h4jf_. All alpha proteins Non-globular all-alpha subunits of globular proteins ... Methylobacterium extorquens [TaxId: 408] H. d1h4jh_. All alpha proteins Non-globular all-alpha subunits of globular proteins ...
Crystal structure of Malyl-CoA lyase from Methylobacterium extorquens. Gonzalez, J.M., Marti-Arbona, R., Unkefer, C.J.. To be ... Crystal structure of Malyl-CoA lyase from Methylobacterium extorquens. *DOI: 10.2210/pdb4ROQ/pdb ...
Methylobacterium populi. Methylobacterium salsuginis. Methylobacterium sp. Leaf456. Methylobacterium zatmanii. Methylobacterium ... Methylobacterium salsuginis. Methylobacterium sp. Leaf456. Methylobacterium sp. Leaf92. Methylobacterium sp. Leaf123. ... Methylobacterium extorquens DSM 13060. Methylobacterium extorquens (strain CM4 / NCIMB 13688) (Methylobacterium ... Methylobacterium extorquens DSM 13060. Methylobacterium extorquens (strain CM4 / NCIMB 13688) (Methylobacterium ...
PhyR has a central role in the adaptation of Methylobacterium to the plant environment. Our proteome analysis points to a ... A proteomic study of Methylobacterium extorquens reveals a response regulator essential for epiphytic growth. Benjamin Gourion ... For this work, we have used the Alphaproteobacterium Methylobacterium extorquens AM1, a well studied model pink-pigmented ... Aerial plant surfaces are colonized by diverse bacteria such as the ubiquitous Methylobacterium spp. The specific physiological ...
Methylobacterium sp. (strain 4-46). ,p>This subsection of the Names and taxonomy section shows the unique identifier assigned ... Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060). 590. UniRef90_B8I9T2. Cluster: Arginine--tRNA ligase. 2 ... sp,B0UI81,SYR_METS4 Arginine--tRNA ligase OS=Methylobacterium sp. (strain 4-46) GN=argS PE=3 SV=1 ...
... are assigned to a novel species within the genus Methylobacterium, and the name Methylobacterium ajmalii sp. nov. is proposed. ... The average nucleotide identity (ANI) values of these three ISS strains were Methylobacterium species. Based on the ANI and ... with any described Methylobacterium species. Based on the ANI and dDDH analyses, these three ISS strains were considered as ... with the closest being Methylobacterium indicum SE2.11T. Furthermore, the multi-locus sequence analysis placed these three ISS ...
V. Gallego, M. T. García, and A. Ventosa, "Methylobacterium hispanicum sp. nov. and Methylobacterium aquaticum sp. nov., ... P. N. Green, I. J. Bousfield, and D. Hood, "Three new Methylobacterium species: M. rhodesianum sp. nov, M. zatmanii sp. nov., ... Unusual Methylobacterium fujisawaense Infection in a Patient with Acute Leukaemia Undergoing Hematopoietic Stem Cell ... A. L. Truant, R. Gulati, O. Giger, V. Satishchandran, and J. G. Caya, "Methylobacterium bactaeremia in AIDS," Clinical ...
... Metab Eng. 2015 ... α-humulene from the abundantly available non-food carbon source methanol by metabolically engineered Methylobacterium ...
Article Production of an insecticidal crystal protein from bacillus thuringiensis by the methylotroph methylobacterium ... Production of an insecticidal crystal protein from bacillus thuringiensis by the methylotroph methylobacterium extorquens. 0 ... We cloned and expressed the cry1Aa gene in a plant-colonizing methylotroph, Methylobacterium extorquens, under the control of ... were found for Production of an insecticidal crystal protein from bacillus thuringiensis by the methylotroph methylobacterium ...
sp,Q9F0C9,NODA_METNO Nodulation protein A OS=Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060) OX=460265 GN ... cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium › ... Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060). ,p>This subsection of the ,a href="http://www.uniprot. ...
Methylobacterium extorquens AM1 is a facultative methylotroph capable of growth on both single-carbon and multi-carbon ... Methylobacterium extorquens AM1, a facultative methylotrophic α-proteobacterium, has been engineered to generate 1-butanol from ... Title: Non-targeted determination of 13C-labeling in the Methylobacterium extorquens AM1 metabolome using the two-dimensional ... "Non-targeted determination of 13C-labeling in the Methylobacterium extorquens AM1 metabolome using the two-dimensional mass ...
Methylobacterium. In A. Balows, H. G. Truper, M. Dworkin, W. Harder, and K. H. Schleifer (ed.), The prokaryotes. Springer- ... Methylotrophy in Methylobacterium extorquens AM1 from a genomic point of view. J. Bacteriol. 185 : 2980-2987. ... Methylobacterium extorquens AM1 grows on one-carbon (C1) compounds using the serine cycle for assimilation (25). This ... Alternative Route for Glyoxylate Consumption during Growth on Two-Carbon Compounds by Methylobacterium extorquens AM1 Yoko ...
Nitrogen metabolism - Methylobacterium sp. 17Sr1-43 Help. [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ...
Two-component system - Methylobacterium sp. 17Sr1-43 [ Pathway menu , Pathway entry , Download KGML , Show description , Image ...
In contrast to growth of Methylobacterium strains with the closely-related compound dichloromethane, we find that chloride ... In contrast to growth of Methylobacterium strains with the closely-related compound dichloromethane, we find that chloride ... as well as Methylobacterium nodulans (Sy et al., 2001) and Methylobacterium radiotolerans (Sanders and Maxcy, 1979) ( ... Methylobacterium strains were grown in M-PIPES at 30°C, supplemented with 3.5 mM succinate for routine growth and with 12.5 μg/ ...
"Methylobacterium terrae" Kim et al. 2019. not validly published. Methylobacterium terricola Kim et al. 2020. validly published ... "Methylobacterium indicum" Chaudhry et al. 2016. not validly published. Methylobacterium iners Weon et al. 2008. validly ... "Methylobacterium planium" Jiang et al. 2020. not validly published. Methylobacterium platani Kang et al. 2007. validly ... "Methylobacterium ajmalii" Bijlani et al. 2021. not validly published. Methylobacterium aminovorans Urakami et al. 1993. validly ...
We report the presence of ACC deaminase in Methylobacterium fujisawaenseand its lowering of ethylene levels and promotion of ... Koenig RL, Morris RO, Polacco JC (2002) tRNA is the source of low-level trans-Zeatin production in Methylobacterium spp. J ... Green PN (1992) The genus Methylobacterium. In: Balows A, Trüper HG, Dworkin M, Harder W, Schleifer KH (eds), The prokaryotes, ... We report the presence of ACC deaminase in Methylobacterium fujisawaense and its lowering of ethylene levels and promotion of ...
The pink-pigmented facultative methylotroph Methylobacterium extorquens AM1 is known to generate a pink carotenoid (6, 11). ... Genetic Characterization of the Carotenoid Biosynthetic Pathway in Methylobacterium extorquens AM1 and Isolation of a Colorless ... Genetic Characterization of the Carotenoid Biosynthetic Pathway in Methylobacterium extorquens AM1 and Isolation of a Colorless ... Genetic Characterization of the Carotenoid Biosynthetic Pathway in Methylobacterium extorquens AM1 and Isolation of a Colorless ...
Complete genome sequences of six strains of the genus Methylobacterium. J. Bacteriol. 194:4746-4748. doi:10.1128/JB.01009-12. ... Methylobacterium extorquens DM4 lacks the MMA dehydrogenase encoded by mau genes, which in M. extorquens AM1 is essential for ... Genes of the N-Methylglutamate Pathway Are Essential for Growth of Methylobacterium extorquens DM4 with Monomethylamine. ... Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and ...
In a recent paper we reported the sequence of the beginning of a serine cycle gene cluster on the Methylobacterium extorquens ... Chistoserdova, Ludmila V. and Lidstrom, Mary E. (1994) Genetics of the serine cycle in Methylobacterium extorquens AM1: ... Genetics of the serine cycle in Methylobacterium extorquens AM1: identification, sequence, and mutation of three new genes ...
The nucleotide sequence and deduced amino acid sequence of the cytochrome cL gene of Methylobacterium extorquens AM1, a novel ... The nucleotide sequence and deduced amino acid sequence of the cytochrome cL gene of Methylobacterium extorquens AM1, a novel ... The nucleotide sequence and deduced amino acid sequence of the cytochrome cL gene of Methylobacterium extorquens AM1, a novel ... The nucleotide sequence and deduced amino acid sequence of the cytochrome cL gene of Methylobacterium extorquens AM1, a novel ...
  • To take advantage of this technology, 60-mer oligonucleotide microarrays were designed for Methylobacterium extorquens AM1 to study gene expression changes that occur under differing physiological conditions. (nih.gov)
  • Methylobacterium extorquens AM1, complete genome. (atcc.org)
  • Here, we use adaptation of experimental populations of the model methylotroph, Methylobacterium extorquens AM1, to C(1) (methanol) or multi-C (succinate) compounds to investigate specialization and trade-offs between these two metabolic lifestyles. (nih.gov)
  • The purpose of this study was to identify proteins produced specifically in the phyllosphere by comparing the proteome of Methylobacterium extorquens colonizing the leaves either with that of bacteria colonizing the roots or with that of bacteria growing on synthetic medium. (pnas.org)
  • For this work, we have used the Alphaproteobacterium Methylobacterium extorquens AM1, a well studied model pink-pigmented facultative methylotroph (PPFM) ( 10 ), whose draft genome sequence is available (see www.integratedgenomics.com/genomereleases.html#6 ). (pnas.org)
  • In this study we present de novo synthesis of the sesquiterpenoid α-humulene from the abundantly available non-food carbon source methanol by metabolically engineered Methylobacterium extorquens AM1. (nih.gov)
  • We cloned and expressed the cry1Aa gene in a plant-colonizing methylotroph, Methylobacterium extorquens, under the control of the strong M. extorquens AM1 methanol dehydrogenase promoter, PmxaF. (environmental-expert.com)
  • Methylobacterium extorquens AM1 is a facultative methylotroph capable of growth on both single-carbon and multi-carbon compounds. (osti.gov)
  • Methylobacterium extorquens AM1 grows on one-carbon (C 1 ) compounds using the serine cycle for assimilation ( 25 ). (asm.org)
  • Genomic searches were used to reconstruct the putative carotenoid biosynthesis pathway in the pink-pigmented facultative methylotroph Methylobacterium extorquens AM1. (asm.org)
  • The pink-pigmented facultative methylotroph Methylobacterium extorquens AM1 is known to generate a pink carotenoid ( 6 , 11 ). (asm.org)
  • Methylobacterium extorquens DM4 lacks the MMA dehydrogenase encoded by mau genes, which in M. extorquens AM1 is essential for growth on MMA. (asm.org)
  • In a recent paper we reported the sequence of the beginning of a serine cycle gene cluster on the Methylobacterium extorquens AM1 chromosome, containing the genes encoding serine glyoxylate aminotransferase (sgaA), hydroxypyruvate reductase (hprA), and 5,10-methylenetetrahydrofolate dehydrogenase (mtdA) (L. V. Chistoserdova and M. E. Lidstrom J. Bacteriol. (caltech.edu)
  • Methylobacterium extorquens AM1 uses dedicated cofactors for one-carbon unit conversion. (pubmedcentralcanada.ca)
  • The importance of "archaeal" cofactors for one-carbon unit conversion was later extended to aerobic bacteria with the discovery of "methanogenic" enzymes in the methylotrophic model bacterium Methylobacterium extorquens AM1. (pubmedcentralcanada.ca)
  • The gene (glyA) of Methylobacterium extorquens AM1 encoding serine hydroxymethyltransferase (SHMT), one of the key enzymes of the serine cycle for C_1 assimilation, was isolated by using a synthetic oligonucleotide with a sequence based on amino acid sequence conserved in SHMTs from different sources. (caltech.edu)
  • Methylobacterium extorquens AM1, a facultative methylotrophic α-proteobacterium, has been engineered to generate 1-butanol from cheap carbon feedstocks through a synthetic metabolic pathway. (biomedcentral.com)
  • Methylobacterium extorquens AM1 is capable of growth on one-carbon compounds such as methanol. (sybirg.re.kr)
  • Heterogeneity of cells within exponentially growing populations was addressed in a bacterium, the facultative methylotroph Methylobacterium extorquens AM1. (mendeley.com)
  • Three previously reported Tn 5 mutants of Methylobacterium extorquens AM1, Cou-1, Cou-3 and Cou-6, which are able to grow on methylamine but not methanol, were characterized by biochemical analyses and complementation tests using two genomic libraries of M. extorquens AM1. (microbiologyresearch.org)
  • Nucleotide sequences of the Methylobacterium extorquens sp. (microbiologyresearch.org)
  • Characterisation of a novel soluble c -type cytochrome in a moxD mutant of Methylobacterium extorquens AM1. (microbiologyresearch.org)
  • Methylobacterium extorquens AM1, a facultative methylotrophic α-proteobacterium, is a model system for assessing the possibility of generating products such as 1-butanol from one-carbon and two-carbon feedstocks. (biomedcentral.com)
  • In this work, we engineered a modified CoA-dependent pathway in Methylobacterium extorquens AM1 to produce 1-butanol. (biomedcentral.com)
  • Methylobacterium extorquens AM1 is a facultative methylotrophic α-proteobacterium capable of using both one-carbon (C1) compounds as well as multi-carbon compounds as sole carbon and energy sources. (biomedcentral.com)
  • In the methylotrophic bacterium Methylobacterium extorquens strain AM1, MxaF, a Ca 2+ -dependent methanol dehydrogenase (MDH), is the main enzyme catalyzing methanol oxidation during growth on methanol. (elsevier.com)
  • Methylobacterium extorquens AM1, a facultative methylotrophic α-proteobacterium, is a model system for assessing the possibility of generating 3-HP from one-carbon feedstock methanol. (biomedcentral.com)
  • In order to improve the efficiency of the PQQ production by Methylobacterium extorquens AM1, the strain was treated by atmospheric and room temperature plasma (ARTP). (bvsalud.org)
  • The remaining isolates were closely related to Methylobacterium extorquens , Bosea vestrii , and Achromobacter xylosoxidans . (cdc.gov)
  • In March 2021, a related new unknown species, tentatively named Methylobacterium ajmalii, associated with three new strains, designated IF7SW-B2T, IIF1SW-B5, and IIF4SW-B5, were reported to have been discovered, for the first time, on the International Space Station. (wikipedia.org)
  • Three new Methylobacterium species: M. rhodesianum sp. (atcc.org)
  • 46.4% with any described Methylobacterium species. (frontiersin.org)
  • Phenotypically, these three ISS strains resemble M. aquaticum and M. terrae since they assimilate similar sugars as sole carbon substrate when compared to other Methylobacterium species. (frontiersin.org)
  • Methylobacterium species are Gram-negative, rod-shaped bacteria. (frontiersin.org)
  • with Methylobacterium organophilum as the type species. (frontiersin.org)
  • Thereafter, 11 species from the genus Methylobacterium were redefined into a new genus proposed as Methylorubrum , based on 16S rRNA gene sequence, multi-locus sequence analysis (MLSA), genomic, and phenotypic data ( Green and Ardley, 2018 ). (frontiersin.org)
  • G. J. Zaharatos, A. Dascal, and M. A. Miller, "Discordant carbapenem susceptibility in Methylobacterium species and its application as a method for phenotypic identification," Journal of Clinical Microbiology , vol. 39, no. 5, pp. 2037-2038, 2001. (hindawi.com)
  • Numerous insertion sequences are present in the genome of strain PR1016A T , which has a larger genome than the four Methylobacterium species described above. (microbiologyresearch.org)
  • The genotypic and phenotypic characteristics indicate that strain PR1016A T represents a novel species of the genus Methylobacterium , for which the name Methylobacterium currus sp. (microbiologyresearch.org)
  • We propose that strain 23e T represents a novel species within the genus Methylobacterium , for which the name Methylobacterium gnaphalii sp. (microbiologyresearch.org)
  • In addition to having multiple incidences of urinary tract infections with Morganella species and Enterococcus species, the patient has also had multiple episodes of bacteremia in the past 2 years with Methylobacterium mesophilicum , Acinetobacter lwoffii , Delftia acidovorans , and another unidentified Gram-negative bacillus. (asm.org)
  • API NE (bioMérieux) identified the organism as Methylobacterium species, which was the same organism that was previously identified 14 months prior. (asm.org)
  • This is a potential problem because, for example, Methylobacterium species exhibit resistance to chlorination and are classified as 'opportunistic pathogens' in dental unit water lines (Tsagkari et al, 2017). (dentistry.co.uk)
  • The ' mox FG ' region encodes four polypeptides in the methanol-oxidizing bacterium Methylobacterium sp. (microbiologyresearch.org)
  • The colonization of Spodoptera frugiperda J.E. Smith larvae and rice seedlings by genetically modified endophytic bacterium Methylobacterium mesophilicum, and also the possible transfer of this bacterium to inside the larva 's body during seedlings consumption were studied. (bvsalud.org)
  • Methylobacterium organophilum cells are able to undergo genetic transformation and become competent for DNA uptake near the end of the exponential growth phase. (wikipedia.org)
  • Methylan, a polysaccharide produced from Methylobacterium organophilum, was chemically modified by adding diethylaminoethyl (DEAE) group to the backbone of methylan. (kisti.re.kr)
  • Construction of broad host range cloning vectors: identification of genes necessary for growth of Methylobacterium organophilum on methanol. (microbiologyresearch.org)
  • and Methylobacterium mesophilicum (Austin and Goodfellow 1979) comb. (bacterio.net)
  • The GenBank/EMBL/DDBJ accession numbers of the 16S rRNA gene sequence of Methylobacterium currus PR1016A T are MH158285-MH158295. (microbiologyresearch.org)
  • Methylobacterium bullatum is a Gram-negative, facultatively methylotrophic, strictly aerobic and non-spore-forming bacteria from the genus of Methylobacterium which has been isolated from the moss Funaria hygrometrica in the Bergpark Wilhelmshöhe near Kassel in Germany. (wikipedia.org)
  • Aerial plant surfaces are colonized by diverse bacteria such as the ubiquitous Methylobacterium spp. (pnas.org)
  • Another taxonomic study classified all other previously known pink-pigmented facultative methylotrophic bacteria under the genus Methylobacterium ( Green and Bousfield, 1982 ). (frontiersin.org)
  • Methylobacterium , a new genus of facultatively methylotrophic bacteria. (bacterio.net)
  • To test a part of the previous model proposed for ACC deaminase producing bacteria with Methylobacterium , ACC levels and various enzyme activities were monitored in canola. (springer.com)
  • Methylobamine, a UVA-absorbing compound from the plant-associated bacteria methylobacterium sp. (elsevier.com)
  • Among the most commonly found bacteria in drinking water are Pseudomonas, Sphingomonas, Methylobacterium, Aeromonas and Acinetobacter. (dentistry.co.uk)
  • Based on the substantial, asymmetric loss of C(1) growth during growth on succinate, we suggest that the long-term maintenance of C(1) metabolism across the genus Methylobacterium requires relatively frequent use of C(1) compounds to prevent rapid loss. (nih.gov)
  • Further studies of the genus Methylobacterium and description of Methylobacterium aminovorans sp. (straininfo.net)
  • predominated in larvae and adults, whereas Methylobacterium was the dominant genus at the pupal stage. (mdpi.com)
  • Genome Sequence of Methylobacterium Sp. (rit.edu)
  • Whole genome sequencing reveals a new genospecies of Methylobacterium sp. (rit.edu)
  • RT "Complete sequence of chromosome of Methylobacterium nodulans ORS RT 2060. (univ-lyon1.fr)
  • Nitrogen metabolism - Methylobacterium sp. (kegg.jp)
  • Methylobacterium rhodinum (Heumann 1962) comb. (bacterio.net)
  • Methylobacterium rhodinum corrig. (dsmz.de)
  • M. Fernandez, Z. Dreyer, M. Hockenberry-Eaton, and C. J. Baker, " Methylobacterium mesophilica as a cause of persistent bacteremia in a child with lymphoma," Pediatric Infectious Disease Journal , vol. 16, no. 10, pp. 1007-1008, 1997. (hindawi.com)
  • Methylobacterium radiotolerans (Ito and Iizuka 1971) comb. (bacterio.net)
  • These promising candidates for colonizing and simultaneously reducing the phytotoxicity of TGF were affiliated with Pseudomonas putida, Serratia plymuthica, Pseudomonas corrugata, Methylobacterium radiotolerans and Coniochaeta ligniaria. (rug.nl)
  • Pseudo-outbreak of Sphingomonas and Methylobacterium sp. (cambridge.org)
  • While subsequent formaldehyde utilization is well-characterized, little is known about the cellular consequences of formaldehyde imbalance in Methylobacterium or the mechanisms used to maintain intracellular homeostasis and avert/repair cellular damage. (umn.edu)
  • Methylobacterium AM1) shows that this cytochrome c is completely different, except for its haem-binding site, from all other cytochromes. (biochemj.org)
  • We report the presence of ACC deaminase in Methylobacterium fujisawaense and its lowering of ethylene levels and promotion of root elongation in canola seedlings under gnotobiotic conditions. (springer.com)
  • Isolation and Identification of Methylobacterium sp. (rit.edu)
  • Asymmetric, bimodal trade-offs during adaptation of Methylobacterium to distinct growth substrates. (nih.gov)
  • Our results collectively suggest that Methylobacterium commonly found in soils, as well as on the surfaces of leaves, seeds, and in the rhizosphere of a wide variety of plants could be better exploited to promote plant growth. (springer.com)
  • Genera include METHYLOBACTERIUM, Protomonas, and Roseomonas. (harvard.edu)

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