Methylenetetrahydrofolate Dehydrogenase (NADP)
Formate-Tetrahydrofolate Ligase
Methenyltetrahydrofolate Cyclohydrolase
Aminohydrolases
Methylenetetrahydrofolate Reductase (NADPH2)
5,10-Methylenetetrahydrofolate Reductase (FADH2)
Oxidoreductases Acting on CH-NH Group Donors
Multienzyme Complexes
Folic Acid
Polymorphism, Genetic
5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase
Genotype
Hyperhomocysteinemia
Formate-tetrahydrofolate ligase, also known as formyltetrahydrofolate synthetase, is an enzyme that catalyzes the reaction between formate and tetrahydrofolate to form formyltetrahydrofolate. This reaction is an important step in the metabolic pathway of one-carbon metabolism, which is involved in the biosynthesis of purines, thymidylate, and methionine. The enzyme requires ATP for its activity and plays a crucial role in maintaining the cellular pool of one-carbon units. Deficiencies in this enzyme can lead to serious health consequences, including megaloblastic anemia and neurological disorders.
Methylenetetrahydrofolate cyclohydrolase is an enzyme that is involved in the metabolism of folate, a type of B vitamin. The official medical definition of this enzyme is:
"An enzyme that catalyzes the conversion of 5,6,7,8-tetrahydrofolate to 5,6,7,8-tetrahydropteroylglutamate, a reaction that involves the cleavage of the carbon-nitrogen bond of the methylene bridge and the formation of a cyclic structure."
This enzyme plays an important role in the synthesis of tetrahydrofolate, which is a cofactor involved in the transfer of one-carbon units in various metabolic reactions. Mutations in the gene that encodes this enzyme can lead to a rare inherited disorder called methylenetetrahydrofolate reductase deficiency, which can cause neurological symptoms and developmental delay.
Aminohydrolases are a class of enzymes that catalyze the hydrolysis of amide bonds and the breakdown of urea, converting it into ammonia and carbon dioxide. They are also known as amidases or urease. These enzymes play an essential role in various biological processes, including nitrogen metabolism and the detoxification of xenobiotics.
Aminohydrolases can be further classified into several subclasses based on their specificity for different types of amide bonds. For example, peptidases are a type of aminohydrolase that specifically hydrolyze peptide bonds in proteins and peptides. Other examples include ureases, which hydrolyze urea, and acylamidases, which hydrolyze acylamides.
Aminohydrolases are widely distributed in nature and can be found in various organisms, including bacteria, fungi, plants, and animals. They have important applications in biotechnology and medicine, such as in the production of pharmaceuticals, the treatment of wastewater, and the diagnosis of genetic disorders.
Tetrahydrofolates (THFs) are a type of folate, which is a form of vitamin B9. Folate is essential for the production and maintenance of new cells, especially in DNA synthesis and methylation. THFs are the active forms of folate in the body and are involved in various metabolic processes, including:
1. The conversion of homocysteine to methionine, an amino acid required for protein synthesis and the formation of S-adenosylmethionine (SAM), a major methyl donor in the body.
2. The transfer of one-carbon units in various metabolic reactions, such as the synthesis of purines and pyrimidines, which are essential components of DNA and RNA.
3. The remethylation of homocysteine to methionine, a process that helps maintain normal homocysteine levels in the body. Elevated homocysteine levels have been linked to an increased risk of cardiovascular disease.
THFs can be obtained from dietary sources, such as leafy green vegetables, legumes, and fortified cereals. They can also be synthesized endogenously in the body through the action of the enzyme dihydrofolate reductase (DHFR), which reduces dihydrofolate (DHF) to THF using NADPH as a cofactor.
Deficiencies in folate or impaired THF metabolism can lead to various health issues, including megaloblastic anemia, neural tube defects during fetal development, and an increased risk of cardiovascular disease due to elevated homocysteine levels.
Oxidoreductases acting on CH-NH group donors are a class of enzymes within the larger group of oxidoreductases, which are responsible for catalyzing oxidation-reduction reactions. Specifically, this subclass of enzymes acts on CH-NH group donors, where the CH-NH group is a chemical functional group consisting of a carbon atom (C) bonded to a nitrogen atom (N) via a single covalent bond.
These enzymes play a crucial role in various biological processes by transferring electrons from the CH-NH group donor to an acceptor molecule, which results in the oxidation of the donor and reduction of the acceptor. This process can lead to the formation or breakdown of chemical bonds, and plays a key role in metabolic pathways such as amino acid degradation and nitrogen fixation.
Examples of enzymes that fall within this class include:
* Amino oxidases, which catalyze the oxidative deamination of amino acids to produce alpha-keto acids, ammonia, and hydrogen peroxide.
* Transaminases, which transfer an amino group from one molecule to another, often in the process of amino acid biosynthesis or degradation.
* Amine oxidoreductases, which catalyze the oxidation of primary amines to aldehydes and secondary amines to ketones, with the concomitant reduction of molecular oxygen to hydrogen peroxide.
Multienzyme complexes are specialized protein structures that consist of multiple enzymes closely associated or bound together, often with other cofactors and regulatory subunits. These complexes facilitate the sequential transfer of substrates along a series of enzymatic reactions, also known as a metabolic pathway. By keeping the enzymes in close proximity, multienzyme complexes enhance reaction efficiency, improve substrate specificity, and maintain proper stoichiometry between different enzymes involved in the pathway. Examples of multienzyme complexes include the pyruvate dehydrogenase complex, the citrate synthase complex, and the fatty acid synthetase complex.
Homocysteine is an amino acid that is formed in the body during the metabolism of another amino acid called methionine. It's an important intermediate in various biochemical reactions, including the synthesis of proteins, neurotransmitters, and other molecules. However, elevated levels of homocysteine in the blood (a condition known as hyperhomocysteinemia) have been linked to several health issues, such as cardiovascular disease, stroke, and cognitive decline.
Homocysteine can be converted back to methionine with the help of vitamin B12 and a cofactor called betaine, or it can be converted to another amino acid called cystathionine with the help of vitamin B6 and folate (vitamin B9). Imbalances in these vitamins and other factors can lead to an increase in homocysteine levels.
It is crucial to maintain normal homocysteine levels for overall health, as high levels may contribute to the development of various diseases. Regular monitoring and maintaining a balanced diet rich in folate, vitamin B6, and vitamin B12 can help regulate homocysteine levels and reduce the risk of related health issues.
Folic acid is the synthetic form of folate, a type of B vitamin (B9). It is widely used in dietary supplements and fortified foods because it is more stable and has a longer shelf life than folate. Folate is essential for normal cell growth and metabolism, and it plays a critical role in the formation of DNA and RNA, the body's genetic material. Folic acid is also crucial during early pregnancy to prevent birth defects of the brain and spine called neural tube defects.
Medical Definition: "Folic acid is the synthetic form of folate (vitamin B9), a water-soluble vitamin involved in DNA synthesis, repair, and methylation. It is used in dietary supplementation and food fortification due to its stability and longer shelf life compared to folate. Folic acid is critical for normal cell growth, development, and red blood cell production."
Genetic polymorphism refers to the occurrence of multiple forms (called alleles) of a particular gene within a population. These variations in the DNA sequence do not generally affect the function or survival of the organism, but they can contribute to differences in traits among individuals. Genetic polymorphisms can be caused by single nucleotide changes (SNPs), insertions or deletions of DNA segments, or other types of genetic rearrangements. They are important for understanding genetic diversity and evolution, as well as for identifying genetic factors that may contribute to disease susceptibility in humans.
5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase is also known as Methionine Synthase. It is a vital enzyme in the human body that plays a crucial role in methionine metabolism and homocysteine regulation.
The medical definition of 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase is as follows:
A enzyme (EC 2.1.1.13) that catalyzes the methylation of homocysteine to methionine, using 5-methyltetrahydrofolate as a methyl donor. This reaction also requires the cofactor vitamin B12 (cobalamin) as a coenzyme. The enzyme is located in the cytosol of cells and is essential for the synthesis of methionine, which is an important amino acid required for various biological processes such as protein synthesis, methylation reactions, and the formation of neurotransmitters.
Deficiency or dysfunction of this enzyme can lead to several health issues, including homocystinuria, a genetic disorder characterized by elevated levels of homocysteine in the blood, which can cause serious complications such as neurological damage, cardiovascular disease, and skeletal abnormalities.
Genotype, in genetics, refers to the complete heritable genetic makeup of an individual organism, including all of its genes. It is the set of instructions contained in an organism's DNA for the development and function of that organism. The genotype is the basis for an individual's inherited traits, and it can be contrasted with an individual's phenotype, which refers to the observable physical or biochemical characteristics of an organism that result from the expression of its genes in combination with environmental influences.
It is important to note that an individual's genotype is not necessarily identical to their genetic sequence. Some genes have multiple forms called alleles, and an individual may inherit different alleles for a given gene from each parent. The combination of alleles that an individual inherits for a particular gene is known as their genotype for that gene.
Understanding an individual's genotype can provide important information about their susceptibility to certain diseases, their response to drugs and other treatments, and their risk of passing on inherited genetic disorders to their offspring.
Hyperhomocysteinemia is a medical condition characterized by an excessively high level of homocysteine, an amino acid, in the blood. Generally, a level of 15 micromoles per liter (μmol/L) or higher is considered elevated.
Homocysteine is a byproduct of methionine metabolism, an essential amino acid obtained from dietary proteins. Normally, homocysteine gets converted back to methionine with the help of vitamin B12 and folate (vitamin B9), or it can be converted to another amino acid, cysteine, with the aid of vitamin B6.
Hyperhomocysteinemia can occur due to genetic defects in these enzymes, nutritional deficiencies of vitamins B12, B6, or folate, renal insufficiency, or aging. High homocysteine levels are associated with increased risks of cardiovascular diseases, including atherosclerosis, thrombosis, and stroke. It may also contribute to neurodegenerative disorders like Alzheimer's disease and cognitive decline.
It is essential to diagnose and manage hyperhomocysteinemia early to prevent potential complications. Treatment typically involves dietary modifications, supplementation of the deficient vitamins, and, in some cases, medication.
L-Lactate Dehydrogenase (LDH) is an enzyme found in various tissues within the body, including the heart, liver, kidneys, muscles, and brain. It plays a crucial role in the process of energy production, particularly during anaerobic conditions when oxygen levels are low.
In the presence of the coenzyme NADH, LDH catalyzes the conversion of pyruvate to lactate, generating NAD+ as a byproduct. Conversely, in the presence of NAD+, LDH can convert lactate back to pyruvate using NADH. This reversible reaction is essential for maintaining the balance between lactate and pyruvate levels within cells.
Elevated blood levels of LDH may indicate tissue damage or injury, as this enzyme can be released into the circulation following cellular breakdown. As a result, LDH is often used as a nonspecific biomarker for various medical conditions, such as myocardial infarction (heart attack), liver disease, muscle damage, and certain types of cancer. However, it's important to note that an isolated increase in LDH does not necessarily pinpoint the exact location or cause of tissue damage, and further diagnostic tests are usually required for confirmation.
Methylenetetrahydrofolate dehydrogenase (NADP+)
Folate
Nicotinamide adenine dinucleotide
List of MeSH codes (D08)
List of enzymes
List of EC numbers (EC 1)
Riboflavin
Pharmacogenomics and Therapeutic Strategies for Dementia
Publication Detail
Investigation of 95 variants identified in a genome-wide study for association with mortality after acute coronary syndrome -...
Biomarkers Search
Methylenetetrahydrofolate dehydrogenase (NADP+) - Wikipedia
EWS-FLI1 reprograms the metabolism of Ewing sarcoma cells via positive regulation of glutamine import and serine-glycine...
BiGG Reaction MTHFD in iECW 1372
Inflammatory response mediates cross-talk with immune function and reveals clinical features in acute myeloid leukemia |...
DeCS
Code System Concept
MeSH Browser
MeSH Browser
ExplorEnz: EC 6.3.4.3
Pre GI: BLASTP Hits
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亞甲基四氫葉酸脫氫?(MTHFD2)對於肺癌癌幹細胞特性和腫瘤
Elza Ibrahim - Fingerprint - Universitas Indonesia
TMU Research Center of Cancer Translational Medicine - Projects - Taipei Medical University
Pharmacological targeting of MTHFD2 suppresses acute myeloid leukemia by inducing thymidine depletion and replication stress<...
Rhea- Annotated reactions database
SACOL RS05470 - AureoWiki
NEW (2004) MESH HEADINGS WITH SCOPE NOTES (UNIT RECORD FORMAT; 10/2/2003
Derme
DeCS 2004 - Changed terms
BiGG Metabolite mlthf c in iSB619
Saccharopine Dehydrogenases | Profiles RNS
Newborn Screening Codes
Bio2Vec
AHY40105 details
Glyceraldehyde-3-phosphate d1
- glyceraldehyde-3-phosphate dehydrogenase. (gsea-msigdb.org)
Mitochondrial1
- Exposure of brown adipose tissue (BAT) to a variety of stimuli ranging from cold to nutraceuticals, drugs and disease states, activated brown adipocytes release exosomes containing a secretome of nucleic acids and proteins cargoes into the circulation, one of which is mitochondrial methylene tetrahydrofolate dehydrogenase (NADP-dependent) 1-like protein (MTHFD1L) that appears to be a consistent biomarker of BAT activation. (silverchair.com)
Oxidoreductase1
- The systematic name of this enzyme class is 5,10-methylenetetrahydrofolate:NADP+ oxidoreductase. (wikipedia.org)
Enzyme3
- In enzymology, a methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) is an enzyme that catalyzes the chemical reaction 5,10-methylenetetrahydrofolate + NADP+ ⇌ {\displaystyle \rightleftharpoons } 5,10-methenyltetrahydrofolate + NADPH + H+ Thus, the two substrates of this enzyme are 5,10-methylenetetrahydrofolate and NADP+, whereas its 3 products are 5,10-methenyltetrahydrofolate, NADPH, and H+. (wikipedia.org)
- Mutations of the MTHFD1 gene may disrupt the activity of the enzyme and cause methylenetetrahydrofolate dehydrogenase 1 deficiency, also known as combined immunodeficiency and megaloblastic anemia with or without hyperhomocysteinemia (CIMAH). (wikipedia.org)
- 3. Pahlich, E. and Joy, K.W. Glutamate dehydrogenase from pea roots: purification and properties of the enzyme. (qmul.ac.uk)
Coenzyme2
Protein1
- protein_coding" "AAC74323","adhE","Escherichia coli","fused acetaldehyde-CoA dehydrogenase/iron-dependent alcohol dehydrogenase/pyruvate-formate lyase deactivase [Ensembl]. (ntu.edu.sg)
Tetrahydrofolate1
- It is an inhibitor of TETRAHYDROFOLATE DEHYDROGENASE and prevents the formation of tetrahydrofolate, necessary for synthesis of thymidylate, an essential component of DNA. (illumina.com)
Process1
- The Hydride Transfer Process in NADP-dependent Methylene-tetrahydromethanopterin Dehydrogenase. (mpg.de)
Methenyltetrahydrofolate cyclohydrolase2
- 11. A polymorphism, R653Q, in the trifunctional enzyme methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase/formyltetrahydrofolate synthetase is a maternal genetic risk factor for neural tube defects: report of the Birth Defects Research Group. (nih.gov)
- In eukaryotes occurs as a trifunctional enzyme also having methylenetetrahydrofolate dehydrogenase (NADP + ) ( EC 1.5.1.5 ) and methenyltetrahydrofolate cyclohydrolase ( EC 3.5.4.9 ) activity. (enzyme-database.org)
Enzyme methylenetetrahydrofolate dehydrogenase1
- The bifunctional enzyme methylenetetrahydrofolate dehydrogenase - cyclohydrolase (FolD) is identified as a potential drug target in Gram-negative bacteria, in particular the troublesome Pseudomonas aeruginosa. (rhea-db.org)
Lactate Dehydrogenase1
- D-lactate dehydrogenase [Ensembl]. (ntu.edu.sg)
NADPH1
- In enzymology, a methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) is an enzyme that catalyzes the chemical reaction 5,10-methylenetetrahydrofolate + NADP+ ⇌ {\displaystyle \rightleftharpoons } 5,10-methenyltetrahydrofolate + NADPH + H+ Thus, the two substrates of this enzyme are 5,10-methylenetetrahydrofolate and NADP+, whereas its 3 products are 5,10-methenyltetrahydrofolate, NADPH, and H+. (wikipedia.org)
Deficiency2
- 15. Biochemical analysis of patients with mutations in MTHFD1 and a diagnosis of methylenetetrahydrofolate dehydrogenase 1 deficiency. (nih.gov)
- Mutations of the MTHFD1 gene may disrupt the activity of the enzyme and cause methylenetetrahydrofolate dehydrogenase 1 deficiency, also known as combined immunodeficiency and megaloblastic anemia with or without hyperhomocysteinemia (CIMAH). (wikipedia.org)
Interproscan2
- ACT domain, D-isomer specific 2-hydroxyacid dehydrogenase [Interproscan]. (ntu.edu.sg)
- Isopropylmalate dehydrogenase-like domain [Interproscan]. (ntu.edu.sg)
Reaction1
- Catalysis of an oxidation-reduction (redox) reaction in which a CH-NH group acts as a hydrogen or electron donor and reduces NAD or NADP. (planteome.org)
Descriptor1
- Saccharopine Dehydrogenases" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (childrensmercy.org)
Publications2
- This graph shows the total number of publications written about "Saccharopine Dehydrogenases" by people in this website by year, and whether "Saccharopine Dehydrogenases" was a major or minor topic of these publications. (childrensmercy.org)
- Below are the most recent publications written about "Saccharopine Dehydrogenases" by people in Profiles. (childrensmercy.org)