Methyl Green
Microsporidia and Candida spores: their discrimination by Calcofluor, trichrome-blue and methylene-blue combination staining. (1/58)
Faeces of immunocompromised patients are often contaminated with the chitin-containing spores of microsporidia and Candida, which exclude the use of the chitin-specific fluorescent brightener Calcofluor white M2R for the identification of microsporidian spores. We developed a combination staining of Calcofluor white M2R with modified trichrome-blue staining and subsequent methylene-blue incubation which permits discrimination between these two types of spores. As a basis for diagnosis, a difference in the fluorescence pattern (365-440 nm) is combined with a difference in the light microscopic staining pattern. Under fluorescence conditions microsporidia spores have a spotted, brilliant white Calcofluor fluorescence and can easily be identified, while Candida spores show a reddish purple colour. Under the light microscope microsporidian spores show a light red colour with nonstained vacuole spots or strips in contrast to the yeast spores with their red-brown colour. This combination technique offers a highly specific means for the diagnosis of microsporidia spores in faeces. (+info)DNA binding and gene activation properties of the Nmp4 nuclear matrix transcription factors. (2/58)
Splice variants of the Nmp4 gene include nuclear matrix transcription factors that regulate the type I collagen alpha1(I) polypeptide chain (COL1A1) promoter and several matrix metalloproteinase (MMP) genes. To date, these are the only Cys(2)His(2) zinc finger proteins known to bind within the minor groove of homopolymeric (dA.dT) DNA. Nmp4 isoforms contain from 5 to 8 Cys(2)His(2) zinc fingers, an SH3-binding domain that overlaps with a putative AT-hook and a polyglutamine-alanine repeat (poly(QA)). To determine the mechanistic significance of Cys(2)His(2) zinc finger association with this unusual consensus DNA binding element, we identified the Nmp4 DNA-binding and transcriptional activation domains. Zinc fingers 2, 3, and 6 mediated association with the homopolymeric (dA.dT) COL1A1/MMP DNA consensus element. The N terminus of the Nmp4 protein exhibited a strong trans-activation capacity when fused to the GAL4 DNA-binding domain, but this activity was masked within the context of the full-length Nmp4-GAL4 DNA-binding domain chimera. However, upon binding to the COL1A1/MMP homopolymeric (dA.dT) element, the native Nmp4 protein up-regulated transcription, and the poly(QA) domain acquired a significant role in trans-activation. We propose that allosteric effects induced upon zinc finger association with the homopolymeric (dA.dT) minor groove confer context-specific functionality to this unusual family of Cys(2)His(2) transcription factors. (+info)Effects of heparin on liver fibrosis in patients with chronic hepatitis B. (3/58)
AIM: To evaluate the effects of heparin on liver fibrosis in patients with chronic hepatitis B. METHODS: Fifty-two cases under study were divided into two groups, group A and group B. The two groups were given regular treatment and heparin/low molecular weight heparin (LMWH) treatment respectively. Hepatic functions, serum hyaluronic acid (HA) and type IV collagen levels were measured before and after the treatment, and six cases were taken liver biopsy twice. RESULTS: After treatment, hepatic functions became significantly better in both groups. Serum HA and type IV collagen levels in group B compared with group A, decreased significantly after treatment. Collagen proliferation also decreased in group B after treatment. CONCLUSION: Heparin/LMWH can inhibit collagen proliferation in liver tissues with hepatitis B. (+info)Use of Hemo-De to eliminate toxic agents used for concentration and trichrome staining of intestinal parasites. (4/58)
In a blind comparison, 465 randomly collected clinical fecal specimens were examined. Hemo-De was found to be an excellent replacement for ethyl acetate in the concentration procedure and for carbol-xylene and xylene in the trichrome staining procedure. Elimination of toxic reagents, combined with its lower cost, makes Hemo-De the preferred choice in routine parasitology examinations. (+info)Methyl green. III. Reaction with desoxyribonucleic acid, stoichiometry, and behavior of the reaction product. (5/58)
1. Methyl green ("ethyl green") C. I. Number 685 was examined and found to behave identically with methyl green C. I. Number 684 (no longer available) in respect to molar extinction coefficient, effect of combination with polymerized DNA, failure to react with depolymerized DNA, and effect of pH. 2. The mass law permits the calculation of P/dye. This is found to be 13 P/dye. The same value is obtained when an excess of methyl green is caused to fade by adjusting the pH to 7.5. 3. The compound formed by methyl green with DNA has the same maximum absorption at 642.5 to 645 mmicro in the pH range 3.5-7.8, whereas the free dye fades markedly above pH 5.0. (+info)Co-localisation of Batrachochytrium dendrobatidis and keratin for enhanced diagnosis of chytridiomycosis in frogs. (6/58)
Chytridiomycosis is a disease of post-metamorphic frogs caused by the fungus Batrachochytrium dendrobatidis and is associated with large declines in frog populations on a global scale. B. dendrobatidis is found only in the keratinised tissues, which include the mouthparts of healthy tadpoles. The epidermis of infected post-metamorphic frogs is thickened (hyperkeratosis) and the superficial layer can sometimes slough. Diagnosis is most commonly performed on stained sections of toe clips or ventral skin. Accurate interpretation can be difficult and requires a high level of expertise, particularly in infected animals exhibiting hyperkeratosis with sloughing. Misdiagnosis can occur when zoosporangia of B. dendrobatidis are shed with the superficial keratin layers. We have developed a staining protocol based on previously described methods to detect both B. dendrobatidis and keratin, to improve the sensitivity and specificity of diagnosis of chytridiomycosis by inexperienced diagnosticians. (+info)Staining of the Reinke crystalloids in the human testis--re-evaluation study. (7/58)
Reinke crystalloid (CR)s are rod or corn shaped structures present in the interstitial cells (Leydig cell) of human testis. Due to existing controversies and because of their importance in histological studies, we decided to reevaluate their staining behavior with various dyes. Earlier it has been shown that hematoxylin and eosin dyes do not stain CRs even though their protein nature remains undisputed. In the present study, sections of testicular tissues embedded in glycol-methacrylate (GMA) showed that hematoxylin binds to CRs non-specifically and eosin stains them specifically. The reasons for stainability and/or non stainability of CRs with hematoxylin, eosin and other dyes are discussed. (+info)Histological staining methods preparatory to laser capture microdissection significantly affect the integrity of the cellular RNA. (8/58)
BACKGROUND: Gene expression profiling by microarray analysis of cells enriched by laser capture microdissection (LCM) faces several technical challenges. Frozen sections yield higher quality RNA than paraffin-imbedded sections, but even with frozen sections, the staining methods used for histological identification of cells of interest could still damage the mRNA in the cells. To study the contribution of staining methods to degradation of results from gene expression profiling of LCM samples, we subjected pellets of the mouse plasma cell tumor cell line TEPC 1165 to direct RNA extraction and to parallel frozen sectioning for LCM and subsequent RNA extraction. We used microarray hybridization analysis to compare gene expression profiles of RNA from cell pellets with gene expression profiles of RNA from frozen sections that had been stained with hematoxylin and eosin (H&E), Nissl Stain (NS), and for immunofluorescence (IF) as well as with the plasma cell-revealing methyl green pyronin (MGP) stain. All RNAs were amplified with two rounds of T7-based in vitro transcription and analyzed by two-color expression analysis on 10-K cDNA microarrays. RESULTS: The MGP-stained samples showed the least introduction of mRNA loss, followed by H&E and immunofluorescence. Nissl staining was significantly more detrimental to gene expression profiles, presumably owing to an aqueous step in which RNA may have been damaged by endogenous or exogenous RNAases. CONCLUSION: RNA damage can occur during the staining steps preparatory to laser capture microdissection, with the consequence of loss of representation of certain genes in microarray hybridization analysis. Inclusion of RNAase inhibitor in aqueous staining solutions appears to be important in protecting RNA from loss of gene transcripts. (+info)Methyl Green is not typically considered a medical term, but it is a chemical compound that has been used in various medical and laboratory contexts. Here's the general definition:
Methyl Green is a basic dye, which is a type of organic compound with positively charged ions (cations). It is commonly used as a biological stain to selectively color certain structures in cells or tissues, such as nucleic acids (DNA and RNA), during microscopic examination.
In the medical field, Methyl Green has been used as a component of some topical ointments for treating superficial bacterial infections. However, its use is not widespread due to the availability of more effective antibiotics.
It's important to note that Methyl Green should not be confused with Methylene Blue, another basic dye that has broader medical applications, such as treating methemoglobinemia and used as a marker in some diagnostic tests.
Nucleic acids are biological macromolecules composed of linear chains of nucleotides. They play crucial roles in the structure and function of cells, serving as the primary information-carrying molecules in all known forms of life. The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is responsible for storing genetic information in a stable form that can be passed down from generation to generation, while RNA plays a key role in translating the genetic code stored in DNA into functional proteins.
Each nucleotide consists of a sugar molecule, a phosphate group, and a nitrogenous base. The sugar in DNA is deoxyribose, while in RNA it is ribose. The nitrogenous bases found in both DNA and RNA include adenine (A), guanine (G), and cytosine (C). Thymine (T) is found in DNA, but uracil (U) takes its place in RNA. These nucleotides are linked together by phosphodiester bonds between the sugar of one nucleotide and the phosphate group of another, forming a long, helical structure with backbones made up of alternating sugar and phosphate groups.
The sequence of these nitrogenous bases along the nucleic acid chain encodes genetic information in the form of codons, which are sets of three consecutive bases that specify particular amino acids or signals for protein synthesis. This information is used to direct the synthesis of proteins through a process called transcription (converting DNA to RNA) and translation (converting RNA to protein).
In summary, nucleic acids are essential biomolecules composed of chains of nucleotides that store, transmit, and express genetic information in cells. They consist of two main types: DNA and RNA, which differ in their sugar type, nitrogenous bases, and functions.
Methyl green
Methyl green-pyronin stain
Tert-Amyl methyl ether
Cyclopentyl methyl ether
Β-Naphthol methyl ether
Methyl acrylate
Methyl cinnamate
Methyl violet
Methyl salicylate
16α-Methyl-11-oxoprednisolone
Methyl orange
Chloromethyl methyl ether
Green solvent
Choline
Silica gel
Janet Scott (scientist)
Agriculture in California
Vitex rotundifolia
Isocyanide
Gaston Milian
Viologen
Garlic
Mercury methylation
Strepsilin
1-Butyl-3-methylimidazolium hexafluorophosphate
Cinnamon basil
Nutriepigenomics
Ethyl Green
Bioconversion of biomass to mixed alcohol fuels
Tetrabromonickelate
Methyl green - Wikipedia
ABC of Safety in the Biological Sciences - Methyl Green
Product recovery from ionic liquids by solvent-resistant nanofiltration: application to ozonation of acetals and methyl oleate ...
Chemical Database: 1H-Indole-3-acetic acid, 2-methyl-, 2-((2,4-dimethoxyphenyl)methyl)hydrazide (EnvironmentalChemistry.com)
Chemical Database: Ethylene dibromide and Methyl bromide mixture, liquid (EnvironmentalChemistry.com)
ARCHIVED - Environment and Climate Change Canada - Evaluating Existing Substances - Assessment report for Benzene, 1-methyl-2...
Molbank | Free Full-Text | 1-{4-[(Hexyloxy)methyl]pyridin-2-yl}ethanone
IUCr) Di-μ-thio-cyanato-κ4N:N-bis--({2,4-di-bromo-6-[2-(methyl-amino)ethyl-imino-meth-yl]-phenol-ato-κ3N,N′,O}nickel(II))
DailyMed - ANTISEPTIC- eucalyptol, menthol, methyl salicylate, thymol mouthwash
Effects of temperature, ultraviolet radiation and pectin methyl esterase on aerobic methane release from plant material - Open...
ethyl methyl anthranilate, 35472-56-1
Frontiers | Anti-N-methyl-D-Aspartate Receptor Encephalitis Mimicking Sporadic Creutzfeldt-Jakob Disease
Molbank | Free Full-Text | Tributyl(1-((dimethylamino)(dimethyliminio)methyl)-1,4-dihydropyridin-4-yl)phosphonium...
Improvement of Thermal and Mechanical Properties of Vietnam Deproteinized Natural Rubber via Graft Copolymerization with Methyl...
Partitioning and transport of total and methyl mercury in the Lower Fox River, Wisconsin | U.S. Geological Survey
Methyl Hydrate - HollyNorth Production Supplies Ltd.
Removing Greasy Smudges From Marble Using The Cotton Swab Method | GSA
FMES-2 Fatty Acid Methyl Esters Ethoxylate Sulfonate CAS 71338-19-24
4-Hydroxy-6-methyl-3-nitro-2-pyridone 4966-90-9 | Tokyo Chemical Industry (India) Pvt. Ltd.
Methyl-CpG-binding protein 2 (MECP2) mutation type is associated with disease severity in Rett syndrome | Journal of Medical...
Search Odors
Alzheimer Disease Medication: Cholinesterase Inhibitors, N-Methyl-D-Aspartate Antagonists, Combination Drugs, Monoclonal...
Conversion of the toxic and hazardous Zanthoxylum armatum seed oil into methyl ester using green and recyclable silver oxide...
Millipore Sigma's 6-Methyl-5-hepten-2-ol, natural | Perfumer & Flavorist
Novel triazole analogs of apigenin‑7‑methyl ether exhibit potent antitumor activity against ovarian carcinoma cells via the...
Miscellaneous Chemicals Labchem
YSL Black Opium Illicit Green Eau de Parfum 75ml | Boots
Green Tea 500 mg, 100 Veggie Caps | Jarrow Formulas
Ester2
Synthesis4
- The Project Gutenberg eBook of Synthesis of 2-methyl-4-selenoquinazolone 2-phenylbenzoselenazole, and Its Derivatives, by Y -Gwan Chen, M. A. (gutenberg.org)
- The Project Gutenberg EBook of Synthesis of 2-methyl-4-selenoquinazolone, 2-phenylbenzoselenazole, and its derivatives, by Y -Gwan Chen This eBook is for the use of anyone anywhere in the United States and most other parts of the world at no cost and with almost no restrictions whatsoever. (gutenberg.org)
- 6. S.H. Pyo, T. Dishisha, S. Dayankac, J. Gerelsaikhan, S. Lundmark, N. Rehnberg, R. Hatti-Kaul, A new route for the synthesis of methacrylic acid from 2-methyl-1,3-propanediol by integrating biotransformation and catalytic dehydration. (lu.se)
- 8. S.H. Pyo, P. Persson, S. Lundmark, R. Hatti-Kaul, Solvent-free lipase-mediated synthesis of six-membered cyclic carbonates from trimethylolpropane and dialkylcarbonates, Green Chem . (lu.se)
Iodide2
- Recent studies have indicated that exposures to methyl iodide (MeI) produce a number of effects in laboratory animals, including fetal toxicity, neurotoxicity, and degeneration of the nasal epithelium. (cdc.gov)
- Recent studies have indicated that exposures to methyl based pharmacokinetic (PBPK) modeling, and evaluat- iodide (MeI) produce a number of effects in laboratory ani- ing the relevance of the observations in animals to human mals, including fetal toxicity, neurotoxicity, and degenera- health. (cdc.gov)
Bromide1
- Inhalation Toxicology Downloaded from informahealthcare.com by CDC Information Center on 07/06/12 alternative to methyl bromide as a fumigant, there is potential here as a sequence of key events and processes, starting for human exposures to MeI. (cdc.gov)
Anthranilate2
- Methyl anthranilate is found in far higher concentrations in labrusca grapes. (enworld.org)
- Being perfectly honest, I had previously thought that Concord grape jelly was laced with artificial sources of methyl anthranilate in order to make it taste more like grape candy (in one of those 'reality is unrealistic' kinds of ways. (enworld.org)
Fatty6
- The Fatty Acid methyl Esters Ethoxylate Sulfonate, a new type sulfate anionic surfactant. (cookingmamacookoff.com)
- Is a reliable global Fatty Acid Methyl Esters Ethoxylate Sulfonate. (cookingmamacookoff.com)
- Prices of Fatty acid methyl esters ethoxylate sulfonate If you want to buy Fatty Acid Methyl Esters Ethoxylate Sulfonate at bulk. (cookingmamacookoff.com)
- The Fatty Acid methyl Esters ethoxylate Sulfonate exhibits excellent penetration, decontamination emulsification, and resistance to hardwater. (cookingmamacookoff.com)
- The Fatty Acid methyl Esters ethoxylate Sulfonate is rich in foam and has good alkali-resistance. (cookingmamacookoff.com)
- Fatty acid methyl esters ethoxylate sulfonate shipping Payment receipt can be used to ship the goods by air, sea, or express as soon a possible. (cookingmamacookoff.com)
425851
- Methyl green (CI 42585) is a cationic or positive charged stain, related to Ethyl Green, that has been used for staining DNA since the 19th century. (wikipedia.org)
Analogs1
- The present study was designed to synthesize a series of novel triazole analogs of the bioactive apigenin‑7‑methyl ether to evaluate its anticancer activity against three human ovarian cancer cell lines. (spandidos-publications.com)
Flavor3
- Acceptance of green tea, a health promoting functional food material, could be increased if its flavor is improved. (academicjournals.org)
- Persimmon seeds and leaves are likely potential options for green tea improvement since they contain a large number of volatile flavor compounds. (academicjournals.org)
- In the present study, amino acids, volatile flavor compounds, and antioxidant potentials of persimmon tea (PT) prepared with green tea were investigated. (academicjournals.org)
Aqueous2
- When excited at 244 or 388 nm in neutral aqueous solution, methyl green produces a fluorescent emission at 488 or 633 nm respectively. (wikipedia.org)
- When binding DNA under neutral aqueous conditions, methyl green also becomes fluorescent in the far red with an excitation maximum of 633 nm and an emission maximum of 677 nm. (wikipedia.org)
CELLULOSE2
- Some of the lifting moose hair was stabilized with dabs of 5% methyl cellulose. (penn.museum)
- Conservation treatment so far has included consolidation of the flaking paint with methyl cellulose, carried out under the binocular microscope. (penn.museum)
ACID2
- In view of the high compatibility between ozone and ionic liquids, two ozone -mediated model reactions were chosen: firstly the oxidation of acetals to esters in the presence of ozone and secondly the ozonation of methyl oleate to monomethyl azelate and pelargonic acid . (rsc.org)
- poly(2-acrylamido-2-methyl-propane sulfonic acid), P-SA. (degruyter.com)
Ethyl Green1
- Double green SF, light green, ethyl green. (ihcworld.com)
Celery1
- It enhances green leafy vegetables like celery and lettuce and leafy spices like cilantro and parsley. (perfumerflavorist.com)
Concentrations2
- Despite elevated Hg(T) concentrations, methyl mercury (MeHg) concentrations were relatively low, suggesting limited bioavailability of Hg(T) associated with sediments.To investigate transport and partitioning processes of HgT in the Fox River, we coupled detailed time series data of total mercury (HgT) at the river mouth with transect sampling in the Lower Fox River. (usgs.gov)
- Despite elevated HgT concentrations, methyl mercury (MeHg) concentrations were relatively low, suggesting limited bioavailability of HgT associated with sediments. (usgs.gov)
Fruity1
- A dark, roasted coffee base, with warm balsamic notes from vanilla bourbon, is contrasted with the fruity and tangy qualities of green mandarin. (boots.com)
Crystal1
- Commercial Methyl green preparations are often contaminated with Crystal violet. (wikipedia.org)
FRACTION1
- Jarrow Formulas ® Green Tea is water extracted and features 50% polyphenols, including 30% catechins, approximately 15% of which are the EGCG fraction. (jarrow.com)
Extract1
- Taking an extract of black and green tea by mouth doesn't improve blood sugar levels in people with diabetes. (medlineplus.gov)
Vegetable2
- Article: Optimised biodiesel production from waste vegetable cooking oil catalysed by green synthesised calcium oxide nanoparticles Journal: International Journal of Renewable Energy Technology (IJRET) 2022 Vol.13 No.4 pp.377 - 395 Abstract: The current study proposes a simple, cost-effective and environmentally friendly technique for producing biodiesel via transesterification of waste vegetable cooking oil using green synthesised CaO nanoparticles, as a catalyst. (inderscience.com)
- The current study proposes a simple, cost-effective and environmentally friendly technique for producing biodiesel via transesterification of waste vegetable cooking oil using green synthesised CaO nanoparticles, as a catalyst. (inderscience.com)
Nanoparticles2
- In the current study, the potential of the novel, toxic and non-edible seed oil of Zanthoxylum armatum was investigated for eco-friendly production of biodiesel catalysed by green nanoparticles of silver oxide. (edu.sa)
- It was concluded from the current investigation that Zanthoxylum armatum is a potential biomass feedstock for the sustainable production of biodiesel using green nanoparticles of silver oxide. (edu.sa)
Chemistry1
- RTIL's have attracted significant attention for green chemistry applications. (cdc.gov)
Grapes1
- I personally prefer green or white grapes myself. (enworld.org)
Laboratory1
- The LabChem 1 Liter bromocresol green-methyl red alcoholic blends well with alkalines is highly flammable in nature and is mainly used in laboratory and manufacturing factories. (thomassci.com)
Ovarian2
- Taken together, these results showed that the apigenein‑7‑methyl ether novel derivative 3d may prove an important lead molecule for the treatment of ovarian cancer. (spandidos-publications.com)
- People who regularly drink tea, including black tea or green tea, seem to have a lower risk of developing ovarian cancer compared to those who never or rarely drink tea. (medlineplus.gov)
Parfum2
- Introducing new YSL Black Opium Illicit Green Eau de Parfum, a fresh, vibrant take on the iconic, best-selling Black Opium. (boots.com)
- Black Opium Illicit Green Eau de Parfum is a bold and unique collaboration between Black Opium Master Perfumer Nathalie Lorson, and award-winning Parisian mixologist Margot Lecarpentier. (boots.com)
Mild1
- It is a mild, green raw material. (cookingmamacookoff.com)
Jasmine1
- Add in the green floral facets of jasmine and the sweetness of fig, and this electrifying combination of ingredients makes for a scent that is at once zesty, sparkling, creamy and sensual, and challenges gender stereotypes in fragrances. (boots.com)
Floral1
- Together, they twisted Black Opium's original captivating floral gourmand scent with juicy green mandarin and creamy fig accord, making for a vibrant mocktail-inspired fragrance that invigorates all the senses. (boots.com)
Properties3
- In this study, we investigated the improvement of the thermal and mechanical properties of Vietnam deproteinized natural rubber (DPNR) via graft copolymerization of methyl methacrylate (MMA). (hindawi.com)
- Fuel properties of biodiesel were investigated and found comparable to international standards of ASTM D-6571 and EN-14214. (edu.sa)
- It has different properties than green tea, which is made from the fresh leaves of the same plant. (medlineplus.gov)
Antioxidant1
- The antioxidant polyphenols in green tea support cardiovascular and immune health. (jarrow.com)
Creamy2
- FEMA# 4884 brings fresh, green and vegetative notes to your application with creamy, waxy nuances. (perfumerflavorist.com)
- Inspired by the world of mixology, Illicit Green has key notes of zesty green mandarin, creamy fig and the signature Black Opium coffee base. (boots.com)
Crystals1
- After keeping the solution in air for 15 days to allow slow evaporation, green block-like crystals were formed. (iucr.org)
Material1
- Therefore, NR is an abundant green material and also an important resource of Vietnam's economy. (hindawi.com)
Powder1
- A green, combustible powder. (ihcworld.com)
Environmental2
- Pursuant to section 74 of the Canadian Environmental Protection Act, 1999 (CEPA 1999), the Ministers of the Environment and of Health have conducted a screening assessment on benzene, 1-methyl-2-nitro- (2-nitrotoluene), Chemical Abstracts Service Registry Number 88-72-2. (gc.ca)
- The Green Impact Index measures the environmental and societal impact of our products based on an overall score of 20 criteria. (renefurterer.com)
Coffee1
- Don't confuse black tea with other caffeine sources, including coffee, oolong tea, and green tea. (medlineplus.gov)
Blue1
- Methyl blue is an irritant to eyes, the respiratory tract and the gastrointestinal tract. (ihcworld.com)
Alternative1
- Methyl green also emerges as an alternative stain for DNA in agarose gels, fluorometric assays and flow cytometry. (wikipedia.org)
Treatment1
- A partial N -methyl-D-aspartate (NMDA) antagonist is approved for treatment of moderate and severe AD. (medscape.com)
Cancer1
- People who drink tea, including black tea and green tea, do not seem to have a lower risk of bladder cancer compared to those who don't drink tea. (medlineplus.gov)