Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Thyroxine: The major hormone derived from the thyroid gland. Thyroxine is synthesized via the iodination of tyrosines (MONOIODOTYROSINE) and the coupling of iodotyrosines (DIIODOTYROSINE) in the THYROGLOBULIN. Thyroxine is released from thyroglobulin by proteolysis and secreted into the blood. Thyroxine is peripherally deiodinated to form TRIIODOTHYRONINE which exerts a broad spectrum of stimulatory effects on cell metabolism.Serum: The clear portion of BLOOD that is left after BLOOD COAGULATION to remove BLOOD CELLS and clotting proteins.Chorionic Gonadotropin, beta Subunit, Human: The beta subunit of human CHORIONIC GONADOTROPIN. Its structure is similar to the beta subunit of LUTEINIZING HORMONE, except for the additional 30 amino acids at the carboxy end with the associated carbohydrate residues. HCG-beta is used as a diagnostic marker for early detection of pregnancy, spontaneous abortion (ABORTION, SPONTANEOUS); ECTOPIC PREGNANCY; HYDATIDIFORM MOLE; CHORIOCARCINOMA; or DOWN SYNDROME.Cell Culture Techniques: Methods for maintaining or growing CELLS in vitro.Culture Media, Serum-Free: CULTURE MEDIA free of serum proteins but including the minimal essential substances required for cell growth. This type of medium avoids the presence of extraneous substances that may affect cell proliferation or unwanted activation of cells.Culture Media, Conditioned: Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis).Pregnancy-Associated Plasma Protein-A: A product of the PLACENTA, and DECIDUA, secreted into the maternal circulation during PREGNANCY. It has been identified as an IGF binding protein (IGFBP)-4 protease that proteolyzes IGFBP-4 and thus increases IGF bioavailability. It is found also in human FIBROBLASTS, ovarian FOLLICULAR FLUID, and GRANULOSA CELLS. The enzyme is a heterotetramer of about 500-kDa.Immunoglobulin Light Chains: Polypeptide chains, consisting of 211 to 217 amino acid residues and having a molecular weight of approximately 22 kDa. There are two major types of light chains, kappa and lambda. Two Ig light chains and two Ig heavy chains (IMMUNOGLOBULIN HEAVY CHAINS) make one immunoglobulin molecule.Triiodothyronine: A T3 thyroid hormone normally synthesized and secreted by the thyroid gland in much smaller quantities than thyroxine (T4). Most T3 is derived from peripheral monodeiodination of T4 at the 5' position of the outer ring of the iodothyronine nucleus. The hormone finally delivered and used by the tissues is mainly T3.Fatty Acids, Nonesterified: FATTY ACIDS found in the plasma that are complexed with SERUM ALBUMIN for transport. These fatty acids are not in glycerol ester form.Thyrotropin: A glycoprotein hormone secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Thyrotropin stimulates THYROID GLAND by increasing the iodide transport, synthesis and release of thyroid hormones (THYROXINE and TRIIODOTHYRONINE). Thyrotropin consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is common in the pituitary glycoprotein hormones (TSH; LUTEINIZING HORMONE and FSH), but the beta subunit is unique and confers its biological specificity.Mass Media: Instruments or technological means of communication that reach large numbers of people with a common message: press, radio, television, etc.Otitis Media: Inflammation of the MIDDLE EAR including the AUDITORY OSSICLES and the EUSTACHIAN TUBE.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Thyroxine-Binding Proteins: Blood proteins that bind to THYROID HORMONES such as THYROXINE and transport them throughout the circulatory system.Nuchal Translucency Measurement: A prenatal ultrasonography measurement of the soft tissue behind the fetal neck. Either the translucent area below the skin in the back of the fetal neck (nuchal translucency) or the distance between occipital bone to the outer skin line (nuchal fold) is measured.Thyroid Function Tests: Blood tests used to evaluate the functioning of the thyroid gland.Hypothyroidism: A syndrome that results from abnormally low secretion of THYROID HORMONES from the THYROID GLAND, leading to a decrease in BASAL METABOLIC RATE. In its most severe form, there is accumulation of MUCOPOLYSACCHARIDES in the SKIN and EDEMA, known as MYXEDEMA.Serum Albumin: A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules.Pregnancy Trimester, First: The beginning third of a human PREGNANCY, from the first day of the last normal menstrual period (MENSTRUATION) through the completion of 14 weeks (98 days) of gestation.Paraproteinemias: A group of related diseases characterized by an unbalanced or disproportionate proliferation of immunoglobulin-producing cells, usually from a single clone. These cells frequently secrete a structurally homogeneous immunoglobulin (M-component) and/or an abnormal immunoglobulin.Radioimmunoassay: Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.Immunoglobulin lambda-Chains: One of the types of light chain subunits of the immunoglobulins with a molecular weight of approximately 22 kDa.Culture Techniques: Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.Thyroid Hormones: Natural hormones secreted by the THYROID GLAND, such as THYROXINE, and their synthetic analogs.Down Syndrome: A chromosome disorder associated either with an extra chromosome 21 or an effective trisomy for chromosome 21. Clinical manifestations include hypotonia, short stature, brachycephaly, upslanting palpebral fissures, epicanthus, Brushfield spots on the iris, protruding tongue, small ears, short, broad hands, fifth finger clinodactyly, Simian crease, and moderate to severe INTELLECTUAL DISABILITY. Cardiac and gastrointestinal malformations, a marked increase in the incidence of LEUKEMIA, and the early onset of ALZHEIMER DISEASE are also associated with this condition. Pathologic features include the development of NEUROFIBRILLARY TANGLES in neurons and the deposition of AMYLOID BETA-PROTEIN, similar to the pathology of ALZHEIMER DISEASE. (Menkes, Textbook of Child Neurology, 5th ed, p213)Thyroid Gland: A highly vascularized endocrine gland consisting of two lobes joined by a thin band of tissue with one lobe on each side of the TRACHEA. It secretes THYROID HORMONES from the follicular cells and CALCITONIN from the parafollicular cells thereby regulating METABOLISM and CALCIUM level in blood, respectively.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Pregnancy: The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Hypoproteinemia: A condition in which total serum protein level is below the normal range. Hypoproteinemia can be caused by protein malabsorption in the gastrointestinal tract, EDEMA, or PROTEINURIA.Biological Markers: Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.Testosterone: A potent androgenic steroid and major product secreted by the LEYDIG CELLS of the TESTIS. Its production is stimulated by LUTEINIZING HORMONE from the PITUITARY GLAND. In turn, testosterone exerts feedback control of the pituitary LH and FSH secretion. Depending on the tissues, testosterone can be further converted to DIHYDROTESTOSTERONE or ESTRADIOL.Blood Protein Electrophoresis: Electrophoresis applied to BLOOD PROTEINS.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Hyperthyroxinemia: Abnormally elevated THYROXINE level in the BLOOD.Nigella sativa: A plant genus of the family RANUNCULACEAE that contains alpha-hederin, a triterpene saponin in the seeds, and is the source of black seed oil.Dose-Response Relationship, Drug: The relationship between the dose of an administered drug and the response of the organism to the drug.Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Kinetics: The rate dynamics in chemical or physical systems.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Blood: The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.Free Radicals: Highly reactive molecules with an unsatisfied electron valence pair. Free radicals are produced in both normal and pathological processes. They are proven or suspected agents of tissue damage in a wide variety of circumstances including radiation, damage from environment chemicals, and aging. Natural and pharmacological prevention of free radical damage is being actively investigated.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Immunoassay: A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.Immunoglobulin kappa-Chains: One of the types of light chains of the immunoglobulins with a molecular weight of approximately 22 kDa.Glucose: A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Agar: A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.Euthyroid Sick Syndromes: Conditions of abnormal THYROID HORMONES release in patients with apparently normal THYROID GLAND during severe systemic illness, physical TRAUMA, and psychiatric disturbances. It can be caused by the loss of endogenous hypothalamic input or by exogenous drug effects. The most common abnormality results in low T3 THYROID HORMONE with progressive decrease in THYROXINE; (T4) and TSH. Elevated T4 with normal T3 may be seen in diseases in which THYROXINE-BINDING GLOBULIN synthesis and release are increased.Bacteriological Techniques: Techniques used in studying bacteria.Hyperthyroidism: Hypersecretion of THYROID HORMONES from the THYROID GLAND. Elevated levels of thyroid hormones increase BASAL METABOLIC RATE.Serum Albumin, Bovine: Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed)Thyrotoxicosis: A hypermetabolic syndrome caused by excess THYROID HORMONES which may come from endogenous or exogenous sources. The endogenous source of hormone may be thyroid HYPERPLASIA; THYROID NEOPLASMS; or hormone-producing extrathyroidal tissue. Thyrotoxicosis is characterized by NERVOUSNESS; TACHYCARDIA; FATIGUE; WEIGHT LOSS; heat intolerance; and excessive SWEATING.Fibroblasts: Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.Insulin: A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1).Nephelometry and Turbidimetry: Chemical analysis based on the phenomenon whereby light, passing through a medium with dispersed particles of a different refractive index from that of the medium, is attenuated in intensity by scattering. In turbidimetry, the intensity of light transmitted through the medium, the unscattered light, is measured. In nephelometry, the intensity of the scattered light is measured, usually, but not necessarily, at right angles to the incident light beam.Social Media: Platforms that provide the ability and tools to create and publish information accessed via the INTERNET. Generally these platforms have three characteristics with content user generated, high degree of interaction between creator and viewer, and easily integrated with other sites.Cell Survival: The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.Myxedema: A condition characterized by a dry, waxy type of swelling (EDEMA) with abnormal deposits of MUCOPOLYSACCHARIDES in the SKIN and other tissues. It is caused by a deficiency of THYROID HORMONES. The skin becomes puffy around the eyes and on the cheeks. The face is dull and expressionless with thickened nose and lips.Thyroid Diseases: Pathological processes involving the THYROID GLAND.Iodide Peroxidase: A hemeprotein that catalyzes the oxidation of the iodide radical to iodine with the subsequent iodination of many organic compounds, particularly proteins. EC 1.11.1.8.Multiple Myeloma: A malignancy of mature PLASMA CELLS engaging in monoclonal immunoglobulin production. It is characterized by hyperglobulinemia, excess Bence-Jones proteins (free monoclonal IMMUNOGLOBULIN LIGHT CHAINS) in the urine, skeletal destruction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIENCY.Prenatal Diagnosis: Determination of the nature of a pathological condition or disease in the postimplantation EMBRYO; FETUS; or pregnant female before birth.Dialysis: A process of selective diffusion through a membrane. It is usually used to separate low-molecular-weight solutes which diffuse through the membrane from the colloidal and high-molecular-weight solutes which do not. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Antithyroid Agents: Agents that are used to treat hyperthyroidism by reducing the excessive production of thyroid hormones.Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)Calcium: A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Reference Values: The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.Amyloidosis: A group of sporadic, familial and/or inherited, degenerative, and infectious disease processes, linked by the common theme of abnormal protein folding and deposition of AMYLOID. As the amyloid deposits enlarge they displace normal tissue structures, causing disruption of function. Various signs and symptoms depend on the location and size of the deposits.Cholesterol: The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils.Thyroiditis, Subacute: Spontaneously remitting inflammatory condition of the THYROID GLAND, characterized by FEVER; MUSCLE WEAKNESS; SORE THROAT; severe thyroid PAIN; and an enlarged damaged gland containing GIANT CELLS. The disease frequently follows a viral infection.Lipids: A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)Temperature: The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).Prospective Studies: Observation of a population for a sufficient number of persons over a sufficient number of years to generate incidence or mortality rates subsequent to the selection of the study group.Blood Glucose: Glucose in blood.Lipid Metabolism: Physiological processes in biosynthesis (anabolism) and degradation (catabolism) of LIPIDS.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Thyrotropin-Releasing Hormone: A tripeptide that stimulates the release of THYROTROPIN and PROLACTIN. It is synthesized by the neurons in the PARAVENTRICULAR NUCLEUS of the HYPOTHALAMUS. After being released into the pituitary portal circulation, TRH (was called TRF) stimulates the release of TSH and PRL from the ANTERIOR PITUITARY GLAND.TriglyceridesMicroscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Communications Media: The means of interchanging or transmitting and receiving information. Historically the media were written: books, journals, newspapers, and other publications; in the modern age the media include, in addition, radio, television, computers, and information networks.Molecular Weight: The sum of the weight of all the atoms in a molecule.Methimazole: A thioureylene antithyroid agent that inhibits the formation of thyroid hormones by interfering with the incorporation of iodine into tyrosyl residues of thyroglobulin. This is done by interfering with the oxidation of iodide ion and iodotyrosyl groups through inhibition of the peroxidase enzyme.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Organ Culture Techniques: A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)Graves Disease: A common form of hyperthyroidism with a diffuse hyperplastic GOITER. It is an autoimmune disorder that produces antibodies against the THYROID STIMULATING HORMONE RECEPTOR. These autoantibodies activate the TSH receptor, thereby stimulating the THYROID GLAND and hypersecretion of THYROID HORMONES. These autoantibodies can also affect the eyes (GRAVES OPHTHALMOPATHY) and the skin (Graves dermopathy).Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Reagent Kits, Diagnostic: Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.Amino Acids: Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Virus Cultivation: Process of growing viruses in live animals, plants, or cultured cells.Embryo Culture Techniques: The technique of maintaining or growing mammalian EMBRYOS in vitro. This method offers an opportunity to observe EMBRYONIC DEVELOPMENT; METABOLISM; and susceptibility to TERATOGENS.Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.Cricetinae: A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.Fetal Diseases: Pathophysiological conditions of the FETUS in the UTERUS. Some fetal diseases may be treated with FETAL THERAPIES.Bacterial Proteins: Proteins found in any species of bacterium.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Epithelial Cells: Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.Contrast Media: Substances used to allow enhanced visualization of tissues.Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Body Weight: The mass or quantity of heaviness of an individual. It is expressed by units of pounds or kilograms.Blastocyst: A post-MORULA preimplantation mammalian embryo that develops from a 32-cell stage into a fluid-filled hollow ball of over a hundred cells. A blastocyst has two distinctive tissues. The outer layer of trophoblasts gives rise to extra-embryonic tissues. The inner cell mass gives rise to the embryonic disc and eventual embryo proper.Glycoproteins: Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.False Positive Reactions: Positive test results in subjects who do not possess the attribute for which the test is conducted. The labeling of healthy persons as diseased when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed)Hydrocortisone: The main glucocorticoid secreted by the ADRENAL CORTEX. Its synthetic counterpart is used, either as an injection or topically, in the treatment of inflammation, allergy, collagen diseases, asthma, adrenocortical deficiency, shock, and some neoplastic conditions.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Maternal Age: The age of the mother in PREGNANCY.Peptones: Derived proteins or mixtures of cleavage products produced by the partial hydrolysis of a native protein either by an acid or by an enzyme. Peptones are readily soluble in water, and are not precipitable by heat, by alkalis, or by saturation with ammonium sulfate. (Dorland, 28th ed)Rats, Sprague-Dawley: A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.Biological Transport: The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Blood Proteins: Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.Evaluation Studies as Topic: Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.Adipose Tissue: Specialized connective tissue composed of fat cells (ADIPOCYTES). It is the site of stored FATS, usually in the form of TRIGLYCERIDES. In mammals, there are two types of adipose tissue, the WHITE FAT and the BROWN FAT. Their relative distributions vary in different species with most adipose tissue being white.Immune Sera: Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.Kidney: Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.Thyroiditis, Autoimmune: Inflammatory disease of the THYROID GLAND due to autoimmune responses leading to lymphocytic infiltration of the gland. It is characterized by the presence of circulating thyroid antigen-specific T-CELLS and thyroid AUTOANTIBODIES. The clinical signs can range from HYPOTHYROIDISM to THYROTOXICOSIS depending on the type of autoimmune thyroiditis.Immunoglobulin G: The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.Tricuspid Valve Insufficiency: Backflow of blood from the RIGHT VENTRICLE into the RIGHT ATRIUM due to imperfect closure of the TRICUSPID VALVE.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Macrophages: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Neck: The part of a human or animal body connecting the HEAD to the rest of the body.Cell Proliferation: All of the processes involved in increasing CELL NUMBER including CELL DIVISION.Virus Replication: The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.Osmolar Concentration: The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Chick Embryo: The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.Cell Line, Tumor: A cell line derived from cultured tumor cells.Models, Biological: Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.Iron: A metallic element with atomic symbol Fe, atomic number 26, and atomic weight 55.85. It is an essential constituent of HEMOGLOBINS; CYTOCHROMES; and IRON-BINDING PROTEINS. It plays a role in cellular redox reactions and in the transport of OXYGEN.Mice, Inbred C57BLRats, Inbred Strains: Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.Carnitine: A constituent of STRIATED MUSCLE and LIVER. It is an amino acid derivative and an essential cofactor for fatty acid metabolism.Cell Count: The number of CELLS of a specific kind, usually measured per unit volume or area of sample.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Alkaline Phosphatase: An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.Blood Physiological Phenomena: Physiological processes and properties of the BLOOD.Microscopy, Electron, Scanning: Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.Rats, Wistar: A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.Hot Temperature: Presence of warmth or heat or a temperature notably higher than an accustomed norm.Collagen: A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).Predictive Value of Tests: In screening and diagnostic tests, the probability that a person with a positive test is a true positive (i.e., has the disease), is referred to as the predictive value of a positive test; whereas, the predictive value of a negative test is the probability that the person with a negative test does not have the disease. Predictive value is related to the sensitivity and specificity of the test.Embryo, Mammalian: The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Carrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.Mice, Inbred BALB COtitis Media, Suppurative: Inflammation of the middle ear with purulent discharge.Biological Assay: A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.TritiumProteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Coculture Techniques: A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.Growth Substances: Signal molecules that are involved in the control of cell growth and differentiation.Fertilization in Vitro: An assisted reproductive technique that includes the direct handling and manipulation of oocytes and sperm to achieve fertilization in vitro.Gestational Age: The age of the conceptus, beginning from the time of FERTILIZATION. In clinical obstetrics, the gestational age is often estimated as the time from the last day of the last MENSTRUATION which is about 2 weeks before OVULATION and fertilization.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Epithelium: One or more layers of EPITHELIAL CELLS, supported by the basal lamina, which covers the inner or outer surfaces of the body.Oxidation-Reduction: A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).Treatment Outcome: Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.Human Growth Hormone: A 191-amino acid polypeptide hormone secreted by the human adenohypophysis (PITUITARY GLAND, ANTERIOR), also known as GH or somatotropin. Synthetic growth hormone, termed somatropin, has replaced the natural form in therapeutic usage such as treatment of dwarfism in children with growth hormone deficiency.Phosphates: Inorganic salts of phosphoric acid.Risk Factors: An aspect of personal behavior or lifestyle, environmental exposure, or inborn or inherited characteristic, which, on the basis of epidemiologic evidence, is known to be associated with a health-related condition considered important to prevent.Cytopathogenic Effect, Viral: Visible morphologic changes in cells infected with viruses. It includes shutdown of cellular RNA and protein synthesis, cell fusion, release of lysosomal enzymes, changes in cell membrane permeability, diffuse changes in intracellular structures, presence of viral inclusion bodies, and chromosomal aberrations. It excludes malignant transformation, which is CELL TRANSFORMATION, VIRAL. Viral cytopathogenic effects provide a valuable method for identifying and classifying the infecting viruses.L-Lactate Dehydrogenase: A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.Fermentation: Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.Methods: A series of steps taken in order to conduct research.Tumor Markers, Biological: Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.Fasting: Abstaining from all food.Chromatography, Gel: Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.Sodium Chloride: A ubiquitous sodium salt that is commonly used to season food.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Estradiol: The 17-beta-isomer of estradiol, an aromatized C18 steroid with hydroxyl group at 3-beta- and 17-beta-position. Estradiol-17-beta is the most potent form of mammalian estrogenic steroids.Cycloheximide: Antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. It acts by inhibiting elongation during protein synthesis.Follow-Up Studies: Studies in which individuals or populations are followed to assess the outcome of exposures, procedures, or effects of a characteristic, e.g., occurrence of disease.Solubility: The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Cell Adhesion: Adherence of cells to surfaces or to other cells.Neurons: The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.Skin: The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.Anaerobiosis: The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Disease Models, Animal: Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Transferrin: An iron-binding beta1-globulin that is synthesized in the LIVER and secreted into the blood. It plays a central role in the transport of IRON throughout the circulation. A variety of transferrin isoforms exist in humans, including some that are considered markers for specific disease states.

*Acholeplasma laidlawii

... may contaminate bovine serum and also occurs in serum-free cell culture media products. The presence of ... may flourish and survive for prolonged periods at refrigeration and ambient temperatures in serum-free cell culture media. ... laidlawii has been identified as a common contaminant of growth media for cell culture. A. laidlawii was first isolated from ... Windsor HM; Windsor GD; Noordergraaf JH (March 2010). "The growth and long term survival of Acholeplasma laidlawii in media ...

*Secretomics

In culture, cells are surrounded by contaminants. Bovine serum from cell culture media and cellular debris can contaminate the ... To remove these contaminants, cells can be washed with PBS or serum-free medium (SFM) before incubating in SFM and collecting ... Supernatant from cells grown in normal medium and cells grown in medium with stable-isotope labeled amino acids is mixed in a 1 ... Some proteins are secreted in low abundance and then diluted further in the cell culture medium or body fluid, making these ...

*Platelet lysate

FBS-free cell culture media, e.g. with Platelet Lysate or chemically defined/ animal component free, are used for cell therapy ... Laboratory use of serum Laner‑Plamberger et al. J Transl Med (2015) 13:354 Iudicone P (Jan 2014). "Pathogen-free, plasma-poor ... Platelet lysate is commonly used for supplementation of basal media in mesenchymal stem cells culture. Prior the use, the ... The included clotting factors require to add heparin to the cell culture media to prevent coagulation during incubation. ...

*Basic fibroblast growth factor

It is necessary in mouse-feeder cell dependent culture systems, as well as in feeder and serum-free culture systems. FGF2, in ... bFGF is a critical component of human embryonic stem cell culture medium; the growth factor is necessary for the cells to ... "A novel chemical-defined medium with bFGF and N2B27 supplements supports undifferentiated growth in human embryonic stem cells ... Soulet F, Al Saati T, Roga S, Amalric F, Bouche G (Nov 2001). "Fibroblast growth factor-2 interacts with free ribosomal protein ...

*QMCF Technology

CHO or HEK293 based cells) for expression in serum-free suspension. Since QMCF plasmids contain antibiotic resistance gene and ... are able to stably replicate and remain inside dividing cells, a selection and growth of the cell culture takes place. This ... Finally, the process is switched to production phase by changing media temperature to 30 ̊C. construction of protein ... QMCF cell lines express Large-T antigen and EBNA-1 proteins which bind the viral sequences on the QMCF plasmid and hence ...

*Explant culture

... carbon dioxide and a growth medium. Serum-free, nutritious medium provides all the nutrients for the cells to grow. Thus cells ... and the pieces are placed in a cell culture dish containing growth media. Over time, progenitor cells migrate out of the tissue ... In biology, explant culture is a technique to organotypically culture cells from a piece or pieces of tissue or organ removed ... Explant culture can also refer to the culturing of the tissue pieces themselves, where cells are left in their surrounding ...

*E-SCREEN

... and the 4 seeding medium is then removed and replaced with the experimental culture medium (phenol red free DMEM with charcoal ... To accomplish the E-SCREEN assay the cells are trypsinized and plated in well culture plates. Cells are allowed to attach for ... with fetal bovine serum (FBS) and phenol red as buffer tracer (culture medium), at 37 °C, in an atmosphere of 5% CO₂ and 95% ... The E-SCREEN cell proliferation assay is performed with the human MCF-7 breast cancer cell line, an established estrogenic cell ...

*Chemically defined medium

GIBCO Perspectives in Cell Culture Invitrogen Guide to Serum-Free Culture TNC Bio list of Chemically Defined products ... Reduced-serum media (commonly 1-5% FBS) Serum-free media (synonymous with Defined media) Protein-free media (no protein but ... There is a clear distinction between serum-free media and chemically defined media. Serum-free media may contain undefined ... Biological immortality Cell culture assays Organ culture Plant tissue culture Tissue culture Jayme DW, Smith SR (2000). "Media ...

*Roswell Park Memorial Institute medium

RPMI 1640 has traditionally been used for the serum-free expansion of human lymphoid cells. RPMI 1640 uses a bicarbonate ... commonly referred to as RPMI medium or RPMI 1640, is a form of medium used in cell culture and tissue culture used for growing ... www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cell-Culture/Mammalian-Cell-Culture/Classical_Media/RPMI. ... Properly supplemented with serum or an adequate serum replacement, RPMI 1640 allows the cultivation of many cell types, ...

*NS0 cell

"Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium". In vitro ... cellular & developmental biology : journal of the Tissue Culture Association. 24 (12): 1223-8. doi:10.1007/bf02624194. PMID ... From this tumor, the P3K cells were isolated and developed into two cell lines, 289-16 and P3-X63. The 289-16 cell line ... Clones from that cell line were isolated and a nonsecreting cell line was identified and was named NS0/1. As myeloma cells, ...

*Raji cell

The culture medium used to grow this cell line is RPMI supplemented with serum. Some characteristics of Raji cell include a ... Raji cells grow as single, non-motile, free-floating (non-adhesion) individuals or doublets to glass. Some cells look elongated ... The limit of sensitivity of this test was 6 mug AHG/ml serum. The ability of Raji cells to detect AHG in serum depended on the ... "Raji cell". Retrieved February 3, 2012. American Type Culture Collection Website. "ATCC-86: Raji Cell". Retrieved February 3, ...

*Cell culture assay

An extract of the material which is being tested is prepared using physiological saline or serum free media (the latter is ... A near confluent layer of fibroblasts are prepared in a culture plate Old cell culture media is removed The cells are covered ... A near confluent layer of fibroblasts are prepared in a culture plate Old cell culture media ([agar] generally) is removed ... which are incubated for 24 hours at 37 degrees Celsius The material is removed The culture media is removed The remaining cells ...

*Neural stem cell

... been used to isolate and expand CNS stem cells by its ability to aggregate and proliferate hmNPCs under serum-free media ... There are two ways to culture the hmNPCs, the adherent monolayer and the neurosphere culture systems. The neurosphere culture ... both daughter cells are also stem cells. In asymmetric division, a stem cells produces one stem cell and one specialized cell. ... Neural stem cells are more specialized than ESCs because they generate only neural cells (neurons or glial cells). During ...

*Fetal bovine serum

The rich variety of proteins in fetal bovine serum maintains cultured cells in a medium in which they can survive, grow, and ... serum free and chemically defined media (CDM) have been developed as a matter of good laboratory practice. Fetal bovine serum ... Fetal bovine serum is the most widely used serum-supplement for the in vitro cell culture of eukaryotic cells. This is due to ... Fetal bovine serum, as with the vast majority of animal serum used in cell culture, is produced from blood collected at ...

*Induced stem cells

Thus, the ability to generate platelet products ex vivo and platelet products lacking HLA antigens in serum-free media would ... "Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells". ... human iPS cell-derived myeloid cell lines as unlimited cell source for dendritic cell-like antigen-presenting cells". Gene ... "Human Somatic Cell Nuclear Transfer Using Adult Cells". Cell Stem Cell. 14 (6): 777-80. doi:10.1016/j.stem.2014.03.015. PMID ...

*Schneider 2 cells

The S2 cells have been shown to grow up to 5.1×107 cells/ml in serum free medium and above 107 cells/ml in basal media such as ... Several media have been developed for culturing insect cell lines with many of them suitable for culturing S2 cells. ... Schneider 2 cells, usually abbreviated as S2 cells, are one of the most commonly used Drosophila melanogaster cell lines. S2 ... ExpreS2ion Biotechnologies have shown a cell concentration of up 7.0x107 cell/ml. S2 cells are often used for expression of ...

*Embryoid body

... and TPO on development of embryonic stem cell-derived mesoderm and blood progenitors in a defined, serum-free media". ... "Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells". ... or the reprogramming of somatic cells to yield induced pluripotent stem cells (iPS).[8][9][10][11] Similar to ESCs cultured in ... Scalable Embryonic Stem Cell Differentiation Culture". Stem Cells. 22 (3): 275-282. doi:10.1634/stemcells.22-3-275. PMID ...

*Embryonic stem cell

The extracted inner cell mass was cultured on fibroblasts treated with mitomycin-c in a medium containing serum and conditioned ... 2005 detailed information about a new stem cell line that was derived from human embryos under completely cell- and serum-free ... The resulting inner cell mass cells are plated onto cells that will supply support. The inner cell mass cells attach and expand ... These cells isolated from the teratocarcinoma replicated and grew in cell culture as a stem cell and are now known as embryonal ...

*Alpha-Parinaric acid

α-Parinaric acid is cytotoxic to human leukemia cells in cell culture at concentrations of 5 μM or less, by sensitizing the ... the process where free radicals react with electrons from cell membrane lipids, resulting in cell damage. It is similarly ... The concentrations of fatty acids in blood serum or plasma can be measured using α-parinaric acid, which will compete for ... as these proteins help protect beer from foam-reducing medium- and long-chain fatty acids. ...

*HL60

... as HL-60 proliferation immediately ceases if either of these compounds is removed from the serum-free culture media. With this ... The HL-60 cultured cell line provides a continuous source of human cells for studying the molecular events of myeloid ... HL-60 cell model was used to study the effect of DNA topoisomerase (topo) IIα and IIβ on differentiation and apoptosis of cells ... The cell line was derived from a 36-year-old woman with acute promyelocytic leukemia at MD Anderson Cancer Center. HL-60 cells ...

*Cellular agriculture

Van der Valk, J (2010). "Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in ... Missing or empty ,title= (help) https://biotechin.asia/2015/12/02/gelzen-inc-making-sustainable-animal-free-gelatin/. Missing ... making milk from cell culture), Clara Foods (making egg whites from cell culture), Gelzen (making gelatin from bacteria and ... After the creation of the cell lines, efforts to remove serum from the growth media are key to the advancement of cellular ...

*Stemcell Technologies

... serum-free and complete medium designed for the feeder-free culture of human embryonic stem cells (ES cells) and induced ... Stem Cell Culture Medium Low Protein Medium for Feeder-Free Culture of Human ES Cells and iPS Cells - TeSR™-E8™ Official ... mFreSR is a serum-free cryopreservation medium for human embryonic stem cells and induced pluripotent stem cells. mTeSR1 is a ... pluripotent stem cells (iPS cells). It is the most-published feeder-free medium for the culture of pluripotent stem cells and ...

*Gaseous signaling molecules

"Homocysteine remethylation during nitrous oxide exposure of cells cultured in media containing various concentrations of ... "Reaction of nitric oxide with the free sulfhydryl group of human serum albumin yields a sulfenic acid and nitrous oxide". ... This effect was shown in lymphocyte cell cultures and in human liver biopsy samples. Nitrous oxide does not bind as a ligand to ... The beta cells of the pancreas in type 1 diabetes produce an excess of the gas, leading to the death of these cells and to a ...

*Trypsinization

This process of cell culture or tissue culture requires a method to dissociate the cells from the container and each other. ... as cell surface proteins will also be cleaved over time and this will affect cell functioning. Trypsin is inhibited by serum ... cells are provided with growth medium comprising the essential nutrients required for proliferation, and the cells adhere to ... alternate reagents formulated from animal origin-free recombinant proteins enable simpler workflows and are gentler on cells. ...
|p||strong|Technical Advantage:|/strong|  Product manufactured under industry-leading standards for low endotoxin content and superior results|/p| |p||br /| Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells.  This research indicated that additions made to BME aided cell propagation.  MEM is modified with higher concentrations of amino acids to more closely approximate the protein composition of mammalian cells.  When supplemented with serum, MEM has been used for cultivation of a wide variety of cells grown in monolayers, such as fibroblasts.  This formulation contains Earles salts and does not contain L-glutamine, phenol red, and sodium bicarbonate.|/p| |p||br /| -  Complete Certificate of Analysis ...
|p||strong|Technical Advantage: |/strong| Product manufactured under industry-leading standards for low endotoxin content and superior results.|br /| Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells.  This research indicated that additions made to BME aided cell propagation.  MEM is modified with higher concentrations of amino acids to more closely approximate the protein composition of mammalian cells.  When supplemented with serum, MEM has been used for cultivation of a wide variety of cells grown in monolayers, such as fibroblasts.  This formulation contains Earles salts and does not contain L-glutamine and phenol red.|br /| -  Complete Certificate of Analysis available for each production lot, along ...
We recently finished our Ask the Expert discussion, "Incorporating recombinant proteins to produce blood-free media formulations for therapeutic cell types". During this Ask the Expert session, we discussed transitioning cells to blood free formulations, cell health and growth in blood-free medium and associated benefits.. Recent advancements in recombinant protein expression have enabled the cost-effective inclusion of recombinant proteins, such as albumin and transferrin in media formulations. These recombinant proteins can effectively replace human plasma or plasma-derived proteins and provide the benefit of reducing variability and risk of contamination from adventitious agents.. In this Ask the Expert session, Randall Alfano, Ph.D., Vice President of Product Development, provided insight on the use of recombinant albumin and transferrin ...
Tissue culture. Dissociated mixed neocortical cell suspensions were prepared from fetal Swiss-Webster mice (gestational age 14-16 d) and plated on a previously established confluent layer of cortical astrocytes (see below) in 24-well tissue culture plates at 1-2 × 105 cells/cm2, generally as described previously (Rose et al., 1993). The initial plating medium was Eagles Minimum Essential Medium (MEM; Earles salts, supplied glutamine-free) supplemented with 10% heat-inactivated horse serum, 10% heat-inactivated fetal bovine serum, glutamine (total, 2 mm), and glucose (total, 21 mm). Cultures were kept in a 37°C/5% CO2 incubator. After 4-6 d in vitro, non-neuronal cell division was halted by exposure to 10−5 m cytosine arabinoside for 1-3 d. The cells were then shifted into a maintenance ...
Cell lines and culture conditions. Human breast cancer cell lines (MDA-MB-231 and MCF-7) and 293T were purchased from the American Type Culture Collection (ATCC). These cell lines were authenticated at ATCC before purchase by standard short tandem repeat DNA-typing methodology. The murine mammary carcinoma cell line E0771 was purchased from BeNa Culture Collection. The Py8119 cell line was provided by Suling Liu (Fudan University, Shanghai, China). The MDA-MB-231, E0771, and 293T cell lines were maintained in Dulbeccos modified Eagle medium (DMEM; Invitrogen) supplemented with 10% FBS (Invitrogen Corp.). The MCF-7 cell line was maintained in Eagles minimum essential medium (Invitrogen Corp.) supplemented with 10% FBS and 0.01 mg/ml human recombinant insulin. The Py8119 cell ...
In this article,study fermentation conditions of the recombinant strains in shake culture,aiming at improving the protein expression and fermenting in 5L fermentation tank. The result indicates that the suitable fermentation conditions for the recombinant cell are as below: the temperature of cell growth and protein expression is 30℃,the methanol feeding is 2%(volume ratio of the medium)per day,the initial pH is 6.2,the induction time is 72h.According to these conditions,the enzyme activity of shake culture is increased by 64% comparing with before optimization. In 5L fermentation cultivate,the enzyme activity reaches 40864U·mL-1,86 times of the shake culture. ...
Looking for online definition of Sabouraud dextrose agar in the Medical Dictionary? Sabouraud dextrose agar explanation free. What is Sabouraud dextrose agar? Meaning of Sabouraud dextrose agar medical term. What does Sabouraud dextrose agar mean?
The effect has been studied of various media, hormones and of amino acids on the membrane potential of rat hepatoma cells in culture measured by microelectrode impalement. Cells in Eagles minimal essential medium plus 5% serum had a value which varied daily from about 5-8 mV, inside negative. The membrane potential of rat hepatocytes was measured to be 8.7 ± 0.2mV, inside negative. The membrane potential of the hepatoma cells was decreased by insulin and increased by glucagon. Membrane potential was unaffected by change of medium to Hanks or Earles balanced salt solutions or deprivation of serum. It was, however, reduced in cells in phosphate-buffered saline and by reduction of pH. The former effect was shown to be due to the higher [Na+] of phosphat-buffered saline as opposed to the other media. Addition of ...
The effects of extracellular folate concentration on intracellular folate and phosphoribosylpyrophosphate (PRPP) levels and the cytotoxicity of methotrexate and 5-fluorouracil were studied in human KB cells grown in fetal bovine serum-supplemented Eagles minimum essential medium, which contained standard high folic acid levels (2.3 µm) (standard or S medium), or folic acid-free serum-supplemented medium containing approximately 4 nm 5-methyltetrahydrofolate (physiological or P medium), a folate level and form more comparable to that in normal human serum. Macrocytosis and prolongation of the doubling time by 150% were observed after 5-10 serial passes in P medium, but after 10-15 serial passes, KB cells became "adapted" to P medium with return of size and doubling time to values indistinguishable from cells maintained in S medium. Cellular ...
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
AIMS--To determine the best medium for the primary isolation of Helicobacter pylori. METHODS--Sixty six gastric mucosal biopsy specimens frozen in 1 ml Cysteine Albimi media with 20% glycerol from 22 histologically proven H pylori infected patients were cultured on brain heart infusion agar (BHIA) with 7% fresh whole defibrinated horse blood, egg yolk agar (EYA), Columbia blood agar-cyclodextrin agar (CBA-Cd), and commercial trypticase soy agar (TSA) supplemented with 5% sheep blood. RESULTS--Successful primary isolation of H pylori was 96% with BHIA, 78% with TSA, 64% for EYA, and 32% with CBA-Cd. Colonies appeared earlier on BHIA (4.7 +/- 0.1 days, 5.3 +/- 0.4 days, 5.3 +/- 0.4 days, and 7.1 +/- 0.9 days for BHIA, TSA, EYA, and CBA-Cd) and there were more colonies on BHIA than on CBA-Cd, EYA or TSA (599 +/- 88, 104 +/- 66, 260 +/- 107, and 358 +/- 89, respectively). CONCLUSIONS--Success of a medium for passage of isolates apparently does ...
0060] The term "cell proliferation control" means a procedure in which cell proliferative capacity is stopped at a desired time, the cell concentration is maintained without significant damage affecting the viability of cells to the cells, and subsequently cell proliferation is restarted at a desired time. In other words, in a method for controlling cell proliferation of the present invention, the proliferation of primate pluripotent stem cells can be inhibited with a maintenance medium for pluripotent stem cells of the present invention, and after a desired period elapsed, the proliferation of primate pluripotent stem cells can be promoted with a culture medium containing glucose. In a method for controlling cell proliferation of the present invention, the growth rate and the morphology of ...
A comparative study was performed to evaluate best practice culture media and enrichment broths for recovering Salmonella species from human stool samples. A total of 1297 human stools were collected and processed in this study. Evaluation of agar media was carried out by direct plating (DP), 1096 stool samples were inoculated on Modified Semisolid Rappaport-Vassiliadis (MSRV), Xylose-Lysine-Deoxycolate (XLD), MacConkey (MAC), and Hektoen Enteric (HE) agars. Evaluation of enrichment broths were carried out by enrichment all 1297 stool samples in Selenite broth (SB), Rappaport-Vassiliadis (RV) broth, and Buffered Peptone Water (BPW), followed by plating on MSRV, MAC, and HE agars. A total of 102 Salmonella-positive stools by DP, 85.3% (87/102) were recovered utilizing MSRV while recovery from XLD, MAC, and HE agars were 34.3% (35/102), 34.3% (35/102), and 29.4% (30/102) respectively. A total 299/1297 stools ...
In order to adapt LaSota strain of Newcastle disease virus (NDV) on chick embryo fibroblast (CEF) cells, 0.1 ml from LaSota vaccine produced in Razi vaccine and serum research institute inoculated to the CEF cells and was passaged five times in the CEF grown in Eagles minimum essential medium (MEM). First to third passages were blind but in fourth passage cytopathic effect of virus was observed. Fifth passage viruses were propagated in SPF embryoneted eggs and then harvested allantoic fluids showed presence of NDV with the titer of 108 EID50/ml. Plaque assays performed on this harvest using two agar overlays one supplemented with trypsin, DEAE dextran and magnesium sulfate, the second with neutral red along with the mentioned supplements, overlaid 72 hours after first one. Eight hours later, plaques of adapted virus appeared with various sizes ranging from 1 mm to 3 mm in the diameter. Discrete plaques were observed in the 10-6 dilution and the calculated ...
The purpose of the present investigation was to explore the effects of well-defined flow conditions on the activity of tissue factor (TF) expressed on the surface of cultured rat vascular smooth muscle cells. Cells were cultured to confluence on Permanox brand slides and stimulated to express TF by a 90 min incubation with fresh growth medium containing 10 percent calf serum. The stimulated cells were then placed in a parallel plate flow chamber and perfused with Hanks Balanced Salt Solution containing factor VIIa, factor X (FX), and calcium. The chamber effluent was collected and assayed for factor Xa (FXa) and the steady-state flux of FXa was calculated. The flux values were 68.73, 94.81, 139.75, 138.19, 316.82, and 592.92 fmole/min/cm2 at wall shear rates of 10, 20, 40, 80, 320, and 1280 s−1 respectively. The FXa flux depended on the wall shear rate to a greater degree ...
Sartorius™ Nutrient Media Pad Sets Diameter: 47mm Sartorius™ Nutrient Media Pad Sets Plates Prepared Microbiology Media
Aliquots of wash fluids were taken from wells 1, 2 and 10 in each wash plate, as well as from the media in which the embryos were held before (virus bath) and after washing (holding medium). All the specimens were processed separately on BHK-21 cells.. Embryos were added to 200 µL Eagles minimum essential medium (EMEM) (BioWhittaker, Cambrex) in Eppendorf tubes (AEC-Amersham, Johannesburg), homogenised for 30 s and centrifuged for 5 min at 9300 rcf using an Eppendorf 5415 R centrifuge. Supernatants were aliquoted in 96-well plates in triplicate in 25-µL volumes per well. A 100-µL volume of BHK-21 cells in EMEM was added and the plates were incubated for 6 days at 37 ºC in an incubator containing 5% CO2 before reading. The cells were monitored for the cytopathic effects of BTV. Negative cell cultures were blind-passaged after 7 days for two additional passages.. Experiment 2. ...
TY - JOUR. T1 - Modulation of collagen synthesis by tumor necrosis factor alpha in cultured vascular smooth muscle cells. AU - Hiraga, Syouichi. AU - Kaji, Toshiyuki. AU - Ueda, Yoshimichi. AU - Zisaki, Fumiko. AU - Iwata, Kazushi. AU - Koizumi, Fumitomo. AU - Okada, Yasunori. AU - Katsuda, Shogo. AU - Nakanishi, Isao. PY - 1999/12/10. Y1 - 1999/12/10. N2 - Collagen synthesis in vascular smooth muscle cells (SMCs) after exposure to tumor necrosis factor alpha (TNF-α) was investigated using a culture system. The synthesis of collagenase-digestible proteins (CDP) and noncollagenous proteins (NCP) was evaluated by the [3H]proline incorporation. It was shown that TNF-α markedly suppresses the incorporation of [3H]proline into both CDP and NCP in confluent cultures of SMCs but not in sparse cultures of the cells. Such a marked ...
Volatile fatty acids produced in Robertsons cooked meat medium by a range of clinically relevant anaerobes were compared by gas liquid chromatography with those produced in blood agar. The same volatile fatty acid profiles were obtained in both media, although the concentration of acids was lower in blood agar. We conclude that detection of volatile fatty acids from a pure culture of an organism on solid medium is practicable and offers advantages over the conventional technique.. ...
Jeeven BioSciences, Inc. Announces the Release of its Neuro-PURE™ Serum Free and Xeno Free Tissue Culture Media. Neuro-PURE™ is the only commercially available 100% Serum Free and Xeno Free Tissue Culture Media on the Market.. Atlanta, Georgia (October 22, 2013) - Jeevan BioSciences, Inc. (www.jeevanbiosciences.com) a start-up biotechnology company co-founded by Dr. Rodney Nash, PhD, a former Neuroscience Post-Doctoral Fellow at Emory University, today announces the commercial release of its first product Neuro - PURETM Tissue Culture Media; the first 100% Serum Free and Xeno Free media product available on the market. ...
Purpose: To test the feasibility of using microparticles for slow and sustained release of active matrix metalloproteinase-3 (MMP-3) to degrade extracellular matrix (ECM) elements such as fibronectin in cultured human trabecular meshwork (TM) cells. Accumulation of ECM materials in the TM is believed to be a contributing factor to intraocular pressure elevation, a major risk factor/cause of primary open angle glaucoma.. Methods: β-casein, with molecular weight (26 kDa) and hydrophobicity similar to those of the active MMP-3 fragment (19.2 kDa), was used for the initial testing. β-casein (2.2 mg) was encapsulated into poly(lactic-co-glycolic acid) (PLGA) microparticles following a double emulsion procedure. The protein amount encapsulated into the microparticles was measured by Bradford protein assay and the encapsulation efficiency was determined. The release test was conducted in Dulbeccos Eagles minimum essential medium (DMEM) without or ...
TY - JOUR. T1 - Lipopolysaccharide extends the lifespan of mouse primary-cultured microglia. AU - Kaneko, Yoko S.. AU - Nakashima, Akira. AU - Mori, Keiji. AU - Nagatsu, Toshiharu. AU - Nagatsu, Ikuko. AU - Ota, Akira. PY - 2009/7/7. Y1 - 2009/7/7. N2 - Microglial activation has been implicated in the recognition and phagocytic removal of degenerating neurons; however, this process must be tightly regulated in the central nervous system, because prolonged activation could damage normal neurons. We report that mouse primary-cultured microglia, which are destined to die within a few days under ordinary culture conditions, can live for more than 1 month when kept activated by lipopolysaccharide (LPS) treatment. Primary-cultured microglia treated with sublethal doses of LPS remained viable, without any measurable increase in apoptotic or necrotic cell death. ...
Purpose: The oral mucosal epithelium shows substantial potential for use in regenerative medicine, including the treatment of ocular surface disease. The rationale for using oral mucosal cells is the possibility of treating bilateral ocular surface stem cell disease without the use of immunosuppression. The present project determines the ideal harvesting site for oral mucosal biopsies and the optimum culture media for these cells.. Methods: Pieces of oral epithelium were used from four locations: buccal mucosa (BM), hard palate (HP), lower lip (LL), and transition zone of the lower lip (TZ) of Sprague-Dawley rats. Explants were grown in four different culture media for six days. The media were 1) RPMI 1640 with 10% heat- inactivated fetal bovine serum (FBS), 2 mM ...
Domesticated (farm) salmonid fishes display an increased willingness to accept risk while foraging, and achieve high growth rates not observed in nature. Theory predicts that elevated growth rates in domestic salmonids will result in greater risk-taking to access abundant food, but low survival in the presence of predators. In replicated whole-lake experiments, we observed that domestic trout (selected for high growth rates) took greater risks while foraging and grew faster than a wild strain. However, survival consequences for greater growth rates depended upon the predation environment. Domestic trout experienced greater survival when risk was low, but lower survival when risk was high. This suggests that animals with high intrinsic growth rates are selected against in populations with abundant predators, explaining the absence of such phenotypes in nature. This is, to our knowledge, the first large-scale field experiment to directly test this theory and simultaneously quantify the initial ...
Hi All - Found this Sterile Liquid Culture Media on eBay. Says it will Multiply Spore Syringe up to 40x. for mushroom spores. I was thinking about getting some and using it with Panaeolus (Copelandia)
TY - JOUR. T1 - Multicenter evaluation of BBL CHROMagar MRSA medium for direct detection of methicillin-resistant Staphylococcus aureus from surveillance cultures of the anterior nares. AU - Flayhart, Diane. AU - Hindler, Janet F.. AU - Bruckner, David A.. AU - Hall, Geraldine. AU - Shrestha, Rabin K.. AU - Vogel, Sherilynn A.. AU - Richter, Sandra S.. AU - Howard, Wanita. AU - Walther, Rhonda. AU - Carroll, Karen C. PY - 2005/11. Y1 - 2005/11. N2 - Active surveillance for methicillin-resistant Staphylococcus aureus (MRSA) is among the strategies recommended by the Society for Healthcare Epidemiology of America for control of nosocomial MRSA infections. Infection control and laboratory personnel desire rapid, sensitive, and inexpensive methods to enhance surveillance activities. A multicenter study was performed to evaluate a new selective and differential chromogenic medium, BBL CHROMagar MRSA (C-MRSA) medium (BD Diagnostics, Sparks, MD), which enables recovery and ...
Bbl sabouraud dextrose agar bbl sabouraud dextrose agar bd. Sabouraud dextrose agar (agar dextrosa sabouraud) licuar sabouraud dextrose agar deeps en tubos a mediante ebullición en baño maría.* candida albicans.. medicine for yeast contamination facet results candida. I requerir un experto en este resolver mi problema. Medicinal drug for yeast contamination aspect effects garlic will help due to candida albicans are usually.. Que significa candidato a magister candida albicans en. Fermenti lattici consistent with candida quali; candida albicans sintomas how long does it take to kill the candida on average? Que significa candidato a magister these.. Candida albicans en agar dextrosa sabouraud. Candida albicans en agar dextrosa sabouraud if you have a susceptible immune device, remedy might be greater hard. Candida albicans en agar dextrosa sabouraud the.. Sabouraud agar wikipedia. Candida albicans in sabouraud agar. Trichophyton terrestre in sabouraud agar. Sabouraud agar ...
Culture conditions play an important role in regulating the genomic integrity of Human Pluripotent Stem Cells (HPSCs). We report that HPSCs cultured in Essential 8 (E8) and mTeSR, two widely used media for feeder-free culturing of HPSCs, had many fold higher levels of ROS and higher mitochondrial potential than cells cultured in Knockout Serum Replacement containing media (KSR). HPSCs also exhibited increased levels of 8-hydroxyguanosine, phospho-histone-H2a.X and p53, as well as increased sensitivity to γ-irradiation in these two media. HPSCs in E8 and mTeSR had increased incidence of changes in their DNA sequence, indicating genotoxic stress, in addition to changes in nucleolar morphology and number. Addition of antioxidants to E8 and ...
Prostate Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC ® Primary Cell Solutions ™ system. This serum-free system is designed to support prostate epithelial cells derived from normal human prostate. Prostate Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of various types of prostate epithelial cells, Prostate Cell Basal Medium must be supplemented with the appropriate cell-specific growth kit. When using this complete media system, the growth of prostate epithelial cells is supported without the use of ...
Global Cell Culture Media Market by Manufacturers, Regions, Type and Application, Forecast to 2021"Purchase This Report by calling ResearchnReports.com at +1-888-631-6977.. Cell Culture Media are used to provide nutrients for cell growth in research, diagnostic and manufacturing applications. Typical cell culture media contain a mixture of defined nutrients dissolved in a buffered physiological saline solution.. Scope of the Report:. This report focuses on the Global Cell Culture Media market, especially in North America, Europe and Asia-Pacific, South America, Middle East and Africa. This report categorizes the market based on manufacturers, regions, type ...
The aim of this work is to investigate the effect of acid treatment -before and during heat inactivation- on the heat resistance of Escherichia coli K12 MG1655 cells at lethal temperatures. E. coli cells were grown in Brain Heart Infusion broth until they reached the stationary phase (≈10(9) cfu/mL). Approximately 30 min before thermal inactivation the early stationary phase cells were added in Brain Heart Infusion broth with a specific pH value, achieved with addition of either acetic (50% (v/v)), lactic (50% (v/v)) or hydrochloric acid (30% (v/v)), and inactivation experiments took place at 54 °C and 58 °C. The inactivation dynamics are analysed using the inactivation model of Geeraerd et al. (2000). This enables to define the induced thermotolerance of E. coli as a prolongation of the shoulder and/or a reduction of the inactivation rate. Generally, addition of acids increased the heat resistance of E. coli. The induced resistance depends on the type of ...
China Muscle Growth Supplement Peg Mechano Growth Powder Peg Mgf, Find details about China Peg Mgf, Powder Peg Mgf from Muscle Growth Supplement Peg Mechano Growth Powder Peg Mgf - Guangzhou Kafen Biotech Co., Ltd.
Profiling of glycosylation gene expression in CHO fed-batch cultures in response to glycosylation-enhancing medium components. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Renal Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC ® Primary Cell Solutions ™ system. This system is designed to support epithelial cells (e.g., renal proximal tubule epithelial cells) derived from normal human kidney. Renal Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of renal epithelial cells (e.g., Primary Renal Proximal Tubule Epithelial Cells, Normal, Human, ATCC ® PCS-400-010; Primary Renal Cortical Epithelial Cells, Normal, Human, ATCC ® PCS-400-011; Primary Renal Mixed Epithelial Cells, Normal, Human, ATCC ® PCS-400-012), Renal ...
Chocolate agar (CHOC) or chocolate blood agar (CBA) - is a nonselective, enriched growth medium used for isolation of pathogenic bacteria. It is a variant of the blood agar plate, containing red blood cells that have been lysed by slowly heating to 80 °C. Chocolate agar is used for growing fastidious respiratory bacteria, such as Haemophilus influenzae and Neisseria meningitidis. In addition, some of these bacteria, most notably H. influenzae, need growth factors such as NAD (factor V) and hemin (factor X), which are inside red blood cells; thus, a prerequisite to growth for these bacteria is lysis of the red blood cells. The heat also inactivates enzymes which could otherwise degrade NAD. The agar is named for the color and contains no actual chocolate. Chocolate agar with the addition of bacitracin becomes selective, it is most critically, for the genus Haemophilus. Another variant of chocolate agar called Thayer-Martin agar contains an assortment of ...
The Department of Media Culture is committed to providing students with a critical understanding of film, media, and communications. We integrate studies in history, theory, and criticism with practical experiences in production technique and creativity as well as external field experience, allowing our undergraduate majors to specialize in cinema studies or film and television production, media studies, corporate communications, journalism, or design and digital media.. ...
Langford test 1 (from quizlet) - infectious diseases 4180 with at muscles. Human Muscle Cell Can Perform Quizlet pleasant in order to the web site, within this moment I will explain to you with regards to Human muscle cell can perform quizlet.. And from now on, this can be the very first picture, human muscle cells can perform quizlet :. ...
Links connect you with a Word document list of the media placements.. NAMI Mass Media Placements - June 2016. NAMI Mass Media Placements - May 2016. NAMI Mass Media Placements - April 2016. NAMI Mass Media Placements - March 2016. NAMI Mass Media Placements - February 2016. NAMI Mass Media Placements - January 2016. NAMI Mass Media Placements - December 2015. NAMI Mass Media Placements - November 2015. NAMI Mass Media Placements - October 2015. NAMI Mass Media Placements - September 2015. NAMI Mass Media Placements - July-August 2015. NAMI Mass Media Placements - June 2015. ...
This study showed that cultivation conditions (medium composition, initial medium pH, aeration rate, inoculum density) affect biomass and polysaccharide production in Ganoderma lucidum strain HAI 447. The synthetic medium (pH 5.5) with maltose at the concentration of 65.0 g L-1, peptone of 0.2%, as well as in the presence of K, Na, and Mg at concentrations of 6.0, 3.0, and 2.0 mM, respectively, presented the best conditions for biomass (29.2 g L-1) and extra- and intracellular polysaccharide (2.9 mg mL(-1) and 73.3 mg g(-1), respectively) production in the analyzed strain. Constant aeration was optimal for biomass and extracellular polysaccharide production, whereas cultivation under stationary conditions was the best for intracellular polysaccharide production. A peak in biomass and polysaccharide production was observed at an inoculum density of 20.0 mL ...
The present study was undertaken to elucidate different requirements for CD2-mediated activation of naive (CD45RO-) and memory (CD45RO+) CD4+ T cells. A mitogenic combination of anti-CD2 (anti-T11(2) and anti-T11(3] mAbs could effectively induce the proliferation of memory CD4+ T cells even in the absence of monocytes. In marked contrast, naive CD4+ T cells did not disclose any proliferative responses to anti-CD2 mAbs, when monocytes were absent in culture. This differential responsiveness of naive and memory CD4+ T cells appeared to be related largely to a difference in IL-6-producing ability between both populations. IL-6 among monocyte-derived cytokines could correct unresponsiveness of naive CD4+ T cells to anti-CD2 stimulation. Unlike naive CD4+ T cells, memory CD4+ T cells produced IL-6 by themselves, with its mRNA ...
In cooperation with the 1st Internal Clinics of the General University Hospital in Prague, we perform long-term cultivation of primary cells from patients with multiple myeloma and plasma cell leukemia. Two cell lines, UHKT-893 and UHKT-944, have already been established and characterized.. ...
Esculin hydrolysis was first described by Rochaix in 1924.(8) Swan first introduced the use of Bile Esculin Agar in 1954.(9) In 1970, Facklam and Moody determined that the use of the bile esculin test was a reliable way of identifying group D streptococci from non-group D streptococci.(3) When using BEA in biochemical testing of group D streptococci, they found that all group D streptococci will blacken this medium.(3) Other researchers have used BEA for the presumptive identification of Enterobacter spp., Klebsiella spp., and Serratia spp., among the Enterobacteriaceae. This medium contains esculin, ferric citrate to provide ferric ions, and 4% oxbile to inhibit most other strains of non-group D streptococci. Esculin is hydrolyzed by group D streptococci to form dextrose and esculin. This compound reacts with the ferric ions contained within the medium, turning the medium from its original amber color to a dark brown to black. Thus the tolerance to the presence of bile and the hydrolysis of ...
We investigated the expression of the alpha- and beta-subunits of the lysosomal enzyme beta-N-acetylhexosaminidase in the BV-2 microglial cell line under different culture conditions. Beta-N-acetylhexosaminidase from BV-2 microglia cells was separated into its constituent isoenzymes on diethylaminoethyl (DEAE) cellulose, and its activity was monitored with 4-methylumbelliferyl-beta-N-acetylglucosamine and 4-methylumbelliferyl-beta-N-acetylglucosamine-6-sulphate substrates. Forms corresponding to the mouse isoenzymes A and B were present in the cells incubated in serum-supplemented medium as well as in serum-free medium. Lipopolysaccharide, a well-known activator of microglia in vitro, added to the BV-2 cells in serum-supplemented medium induced a decrease in the specific enzymatic activity determined with the ...
Preliminary work has been done on the formulation of a new solventogenic clostridial fermentation medium based on the use of corn steep liquor (CSL). CSL is a by-product of the corn wet-milling industry, and has been used primarily as a feed supplement in the livestock industry. This nutrient-rich medium is an ideal base for use in a bacterial fermentation medium. A medium developed from CSL has been found to support good bacterial growth while allowing a level of solvent production approaching that of complex, more expensive clostridial growth media. When used in combination with C. beijerinckii, BA101, this newly developed medium holds promise to increase the cost-effectiveness of the ABE fermentation. (Abstract shortened by UMI ...
The production of extra cellular lipase in Solid State Fermentation (SSF) using Yarrowia lipolytica NCIM 3589 with Palm Kernal cake (Elaeis guineensis) has been studied. Different parameters such as incubation time, inoculum level, initial moisture content, carbon level and nitrogen level of the medium were optimized. Screening of various process variables has been accomplished with the help of Plackett-Burman design. The maximum lipase activity of 18.58 units per gram of dry fermented substrate (U/gds) was observed with the substrate of Palm Kernal cake in four days of fermentation.
TOONPOOL Cartoons - FINGERPRINTS by fcartoons, tagged officer, exhibition, artwork, pro, museum, art, policeman, police, detective, sea, water, fish, fingerprint, finger, fingerprints, see, meer, ozean, polizist, detektiv, kommissar, hut, kippe, zigarette, fingerabdruck, mütze, ausstellung - Category Media & Culture - rated 3.78 / 5.00
TOONPOOL Cartoons - Downloads by toons, tagged computer, cafe, downloads, toilets, restrooms, computers, coffee, computer, cafe, downloads, toilets, restrooms, computers, coffee - Category Media & Culture - rated 5.00 / 5.00
We reviewed reports of false-positive cultures for Mycobacterium tuberculosis and here propose guidelines for detecting and managing patients with possible false-positive cultures. Mechanisms of false-positive cultures included contamination of clinical devices, clerical errors, and laboratory cross-contamination. False-positive cultures were identified in 13 (93%) of the 14 studies that evaluated ⩾100 patients; the median false-positive rate was 3.1% (interquartile range, 2.2%-10.5%). Of the 236 patients with false-positive cultures reported in sufficient detail, 158 (67%) were treated, some of whom had toxicity from therapy, as well as unnecessary hospitalizations, tests, and contact investigations. Having a single positive culture was a sensitive but nonspecific criterion ...
In 106 Stenotrophomonas maltophilia clinical strains the susceptibility to 19 kinds of antibiotics was tested by the broth dilution micromethod at 24 h and 48 h incubation. Isolated strains demonstrated the lowest frequency of resistance to cotrimoxazole (7.5% of resistant strains at 24 h incubation and 18.9% at 48 h), ofloxacin (13.2% and 30.2%), ciprofloxacin (19.8% and 50.9%) and to cefoperazone/sulbactam (20.8% and 37.7%). The smallest growth of the number of resistant strains after extended incubation was recorded in gentamicin (by 10.4%), ceftazidime (by 11.3%) and cotrimoxazole (by 11.4%). On the contrary, the largest growth of resistance was demonstrated in cefoperazone and ciprofloxacin (by 31.1%). Average values of the growth of minimum inhibitory concentrations (MICs) were lowest in ciprofloxacin and ofloxacin (2.3 times) and highest in piperacillin/tazobactam (4.5 times) and piperacillin (5.0 times). As far as the stability of MIC is concerned, the largest occurrence of strains with the MIC
Abstract: The effects of superoxide-generating agents on the growth and viability of Escherichia coli was investigated using two different strains, a lab strain (ER2566) and a clinical isolate (ATCC 4157). Endogenous superoxide was generated using the redoxcycling agent paraquat, while exogenous superoxide was generated using the xanthine/xanthine oxidase (X/XO) enzyme system. Using optical density measurements to monitor culture growth, the bacteriostatic effect of paraquat was tested in three different growth media: Luria-Bertani broth, double strength Luria-Bertani broth, and nutrient broth. For both strains, paraquat toxicity was greatest in nutrient broth, with toxicity in each medium dependent upon the time of paraquat addition following inoculation. Protection against paraquat toxicity by salts and yeast extract was suggested by the differences observed between growth rates of ER2566 treated cultures ...
Cystic fibrosis (CF) is a disease that causes airway blockage and infection that damages the lung. The lungs of CF patients are frequently loaded with inflammatory cells, damaging proteins and oxidants. Oxidants are molecules that contain oxygen and are capable of disrupting cells and tissue. The CF protein is involved in the transport of the important antioxidant glutathione (GSH). Antioxidants are molecules that block oxidants and render them inactive. The absence of this protection in the airways makes them prone to damage from oxidants. Sodium pyruvate is part of the bodys natural anti-oxidant defense system. Sodium pyruvate reacts directly with oxygen radicals to neutralize them and increases cellular levels of glutathione.. The purpose of this study is to investigate the safety and tolerability of 3 different single dose levels of sodium pyruvate administered via a nebulizer to persons with CF lung disease.. This will be a phase I study with 3 stages. ...
evaluation of increase characteristics on blood agar and. Assessment of increase traits on blood agar candida albicans and candida (torulopsis) glabrata identical day definitive identity of candida albicans, Candida albicans colony morphology on blood agar. Candida albicans colony morphology on blood agar very a waste of money 11/9/2009 customer review by denrecnoc candida albicans colony […]. ...
This study documents the presence of type 1 fimbriae on Shigella and confirms these mannose-sensitive adherence structures to be bona fide components of the Shigella surface. While laboratory-passaged Shigella strains and lyophilized clinical isolates failed to express type 1 fimbriae, 6 of 20 recent clinical isolates, including 4 Shigella flexneri strains, 1 Shigella boydii strain, and 1 Shigella dysenteriae strain, produced type 1 fimbriae as detected by mannose-sensitive hemagglutination (MSHA) and electron microscopy. Optimal production of a predominantly Fim+ population required serial passage every 48 to 72 h in unshaken brain heart infusion broth at 37 degrees C. Fim+ Shigella cultures were capable of reversibly switching to a non-MSHA, afimbriated phase during serial aerobic cultivation on tryptic soy agar plates. The amino acid sequence of S. flexneri type 1 FimA contained 18 substitutions compared to that of Escherichia coli fimbrillin. Indirect immunoelectron ...
A membrane bioreactor was developed to counteract the inhibition effect of furfural in ethanol production. Furfural, a major inhibitor in lignocellulosic hydrolyzates, is a highly toxic substance which is formed from pentose sugars released during the acidic degradation of lignocellulosic materials. Continuous cultivations with complete cell retention were performed at a high dilution rate of 0.5 h−1. Furfural was added directly into the bioreactor by pulse injection or by addition into the feed medium to obtain furfural concentrations ranging from 0.1 to 21.8 g L−1. At all pulse injections of furfural, the yeast was able to convert the furfural very rapidly by in situ detoxification. When injecting 21.8 g L−1 furfural to the cultivation, the yeast converted it by a specific conversion rate of 0.35 g g−1 h−1. At high cell density, Saccharomyces cerevisiae could tolerate very high furfural levels without major changes in the ethanol ...
In a recent study, researchers used our MEA chip cell-culture system to culture the hiPSC-Derived Sensory Neuron Progenitors, along with our Sensory Neuron Maintenance Medium and coating reagents. The study also used our step-by-step guideline to help with the set up and maintenance of the cell-culture itself, along with our MEA system guideline to assist with culturing the hiPSC-Derived Sensory Neuron Progenitors on an MEA system. One key part of the protocol in particular, is to remove the non-neuronal population. This ensures a more homogeneous population of sensory neurons and is simple to do with the addition of mitomycin C to the Sensory Neuron Maintenance Medium after two days of culture. You should see the full effects of growth arrest after seven days. The hiPSC-Derived Sensory Neuron Progenitors must then be maintained in the ...
Gradual increasing of glycerol concentration up to 10% using sheep ruminal fluid as an inoculum for in vitro cultivation was accompanied by significant changes in bacterial population as documented by DGGE analysis. The resulting bacterial consortium was composed of three dominant bacteria with Actinomyces related bacterium to be predominant. Upon cultivation on media with glycerol as a sole carbon source a single bacterium was cultivated from this consortium. Isolate G10 was found to be anaerobic, Gram-positive rod-shaped bacterium. Phylogenetic analysis based on 16S rRNA gene sequences showed that G10 isolate is related to the Actinomyces ruminicola species (97.7% of similarity). The role of rumen actinobacteria is largely unknown and their participation in glycerol utilization (tolerance) has not been described yet. The G10 bacterium and related consortium could be possibly used to improve glycerol tolerance and uptake by ruminants ...
Batch fermentation was performed to investigate the effects of pH and temperature on the butanol production of Clostridium pasteurianum DSM 525 using a synthetic medium containing glycerol as a sole source of carbon and energy. C. pasteurianum DSM 525 showed a good growth at the initial glycerol concentration of 40 g/L, the temperature of 37 ℃, and a pH range from 5.0 to 6.5. The growth rate and the proportion of butanol among the fermentation products increased as the culture pH decreased, but the final glycerol consumption decreased at lower pHs, probably due to the reduced tolerance to butanol at lower pHs. The growth rate and the proportion of butanol increased as the temperature of the medium increased from 25, to 30, and to 37 ℃. However, more butanol was produced at lower temperatures (18.7, 16.8, and 11.5 g/L of butanol were finally produced at the temperatures of 25, 30, and 37 ℃, respectively), probably due to the reduced toxicity of butanol at lower temperatures. ...
Abstract The assay of Rickettsia tsutsugamushi infectivity by plaquing has been improved substantially by a number of changes which were based on our understanding of factors which enhance scrub typhus rickettsial infection of, and replication in, cultured cells. Greater numbers of plaques and/or larger plaques resulted from: 1) use of tissue culture medium instead of brain heart infusion broth as the rickettsial diluent; 2) plaquing in a contact-inhibited mouse embryo cell line rather than in growth-inhibited or uninhibited Vero cells; 3) infection and incubation of monolayers at 35°C instead of at lower temperatures; 4) frequent feeding of infected cultures with medium containing ample amounts of serum; and 5) inclusion of chicken serum in the overlay medium. Plaquing in 24-well tissue ...
Fed-batch culture is, in the broadest sense, defined as an operational technique in biotechnological processes where one or more nutrients (substrates) are fed (supplied) to the bioreactor during cultivation and in which the product(s) remain in the bioreactor until the end of the run. An alternative description of the method is that of a culture in which "a base medium supports initial cell culture and a feed medium is added to prevent nutrient depletion". It is also a type of semi-batch culture. In some cases, all the nutrients are fed into the bioreactor. The advantage of the fed-batch culture is that one can control concentration of fed-substrate in the culture liquid at arbitrarily desired levels (in many cases, at low levels). Generally speaking, fed-batch ...
Looking for purpose of membrane filter technique? Here you can find the lowest price products about purpose of membrane filter technique. We Provide for you about purpose of membrane filter technique page1
The acrosome reaction of epididymal spermatozoa and fertilization in vitro of mouse eggs in chemically defined media without tissue fluid were investigated. About 8 to 10% of motile spermatozoa lost their acrosome but no eggs were penetrated when the spermatozoa and eggs were incubated in a basic medium (modified Krebs-Ringer bicarbonate solution containing glucose) for 5 to 7 hr. Addition of a single metabolic intermediate, such as sodium oxaloacetate or sodium pyruvate, to the basic medium increased the proportion of motile spermatozoa without an acrosome (19 to 34%) and the proportion of eggs penetrated (3·2 to 25·5%). Incubation of spermatozoa and eggs in the basic medium containing serum albumin of various species caused a further increase in the proportion of motile spermatozoa without an acrosome (50 to 65%) and in that of penetrated eggs (60·7 to 86%). The best medium for sperm capacitation and fertilization of mouse ...
The optimal production of P. haemolytica leukotoxin in the culture supernatant of a fluid medium is dependent on a number of factors. The leukotoxin has to be produced by using a strain that is known for its ability to produce high quantities of leukotoxin, inoculated into the most suitable type of medium at the correct culture density containing the necessary supplements and harvested after a certain growth period. The volume in which it is produced may also have an influence. Two different procedures are described to produce the leukotoxin in 5 to 15-ℓ quantities in RPMI 1640 medium. The first method used to produce leukotoxin is one that has been repeatedly described since the presence of the leukotoxin was first established in 1978. Using this method seven batches of leukotoxin were produced in litre quantities with leukotoxin activity ranging from 23-67 u/mℓ. The seed culture inoculum is prepared in ...
Looking for online definition of Thayer-Martin agar in the Medical Dictionary? Thayer-Martin agar explanation free. What is Thayer-Martin agar? Meaning of Thayer-Martin agar medical term. What does Thayer-Martin agar mean?
Sabouraud Dextrose Agar was formulated by Sabouraud in 1892, for culturing dermatophytes.(6) The pH is adjusted to approximately 5.6 in order to enhance the growth of fungi, especially dermatophytes, and to slightly inhibit bacterial growth. This medium is recommended for mold and yeast counts by the United States Pharmacopeia, Standard Methods for the Examination of Water and Wastewater, the Association of Official Analytical Chemists, and the Compendium of Methods for the Microbiological Examination of Foods.(1-3,7,8). Sabouraud Dextrose (SabDex) Agar with Lecithin and Tween® 80 contains digests of animal tissues (peptones), which provide a nutritious source of amino acids and nitrogenous compounds for the growth of fungi and yeasts. Dextrose is added as the energy and carbon source. Lecithin and Tween® 80 serve to neutralize quarternary ammonium compounds and phenolic disinfectants, respectively.. Sabouraud Dextrose (SabDex) Agar with Lecithin and Tween® 80 Contact Plates are used to ...
Brand™ Soda Lime Glass Culture Tubes with Screw Cap Dimensions: 16 dia. x 100mmL Brand™ Soda Lime Glass Culture Tubes with Screw Cap Glass Cell...
The aim of this study was to examine effects of sodium pyruvate on viability as well as on synthesis of reactive oxygen species (ROS), lipid peroxidation and DNA integrity of cryopreserved bovine sperm. In each of 23 Simmental AI bulls three ejaculates were collected. In a split sample design ejaculates were diluted by using Triladyl(®) extender without and with the addition of 5mM sodium pyruvate. Both aliquots were equilibrated for 24h before freezing. Frozen sperm samples were thawed, and examined immediately after thawing (0h) as well as after 3, 6, 12, and 24h incubation at 37°C. The percentages of rapidly motile sperm (RMS), plasma membrane and acrosome intact sperm (PMAI), sperm with a high mitochondrial membrane potential (HMMP), amounts of ROS synthesis (dichlorofluorescein-diacetate (DCFH), CellROX Deep Red Reagent(®) probe (CellROX)) and lipid peroxidation of sperm (LPO) and percentage of sperm with a high degree ...
Background: Non-tuberculous mycobacteria (NTM) pulmonary infections are an emerging issue in the cystic fibrosis (CF) population. In Europe, Mycobacterium abscessus complex (MABSC), which may cause accelerated lung function decline, is the predominant species of NTM in patients with CF. In our clinical practice, isolation of mycobacteria consists of culture of decontaminated sputum on solid media (Lowenstein medium) and automated liquid broth method (MGIT™, Becton Dickinson®). Unfortunately, due to bacteria and fungi overgrowth, isolation of mycobacteria from sputum of these patients remains challenging. Indeed, pulmonary tract of patients with cystic fibrosis is known to be frequently colonised by microorganisms which grow faster and resist to decontamination. RGM medium is a novel agar-based culture medium which contains growth factors and selective agents to allow isolation of rapidly-growing ...
For the diagnosis of prosthetic joint infection (PJI) automated BACTEC™ blood culture bottle methods have comparable sensitivity, specificity and a shorter time to positivity than traditional cooked meat enrichment broth methods. We evaluate the culture incubation period required to maximise sensitivity and specificity of microbiological diagnosis, and the ability of BACTEC™ to detect slow growing Propionibacteria spp. Multiple periprosthetic tissue samples taken by a standardised method from 332 patients undergoing prosthetic joint revision arthroplasty were cultured for 14 days, using a BD BACTEC™ instrumented blood culture system, in a prospective study from 1st January to 31st August 2012. The
Objectives: Removable surface-protecting disposable plastic barriers and disinfectant solutions are commonly used in dental clinics in order to sterilize surfaces and instruments from contaminations. In this study we isolated and identified 3 distinct bacteria from plastic barriers at the dental clinic, and compared the bactericidal effect of 5 different commercially available disinfectants on these bacteria. Methods: After dental procedures, bacteria were collected from surface-protecting plastic barriers at the dental clinic. Bacteria were cultured aerobically on Trypticase Soy Agar plates at 370C for 24hr, and 16S RNA analysis was used for the identification of 3 isolated and selected red, yellow, and white bacterial colonies. Bacteria were streaked on agar plates, exposed to the aerosol spray of Cavicide, Micrylium, OPTIM 33TB, UNISEPTA Plus and PureGreen-24 disinfectant solutions. Plates were incubated for 24hr to determine the extent of bacterial growth on the agar ...
HORADAGODA, N. U., DE ALWIS, M. C. L., WETTIMUNY, S. G. D. S., & VIPULASIRI, A. A. (2017). BIOCHEMICAL AND CULTURAL CHARACTERISTICS OF PASTEURELLA HAEMOLYTICA ISOLATED FROM PNEUMONIC LUNGS OF GOATS. Journal of the National Science Foundation of Sri Lanka, 22(4), 325-333. DOI: http://doi.org/10.4038/jnsfsr.v22i4.8135. ...
Biofilms have been implicated as an important reservoir for pathogens and commensal enteric bacteria such as Escherichia coli in natural and engineered water systems. However, the processes that regulate the survival of E. coli in aquatic biofilms have not been thoroughly studied. We examined the effects of hydrodynamic shear and nutrient concentrations on E. coli colonization of pre-established Pseudomonas aeruginosa biofilms, co-inoculation of E. coli and P. aeruginosa biofilms, and P. aeruginosa colonization of pre-established E. coli biofilms. In nutritionally-limited R2A medium, E. coli dominated biofilms when co-inoculated with P. aeruginosa, and successfully colonized and overgrew pre-established P. aeruginosa biofilms. In more enriched media, P. aeruginosa formed larger clusters, but E. coli still extensively overgrew and colonized the interior of P. aeruginosa clusters. In mono-culture, E. coli formed sparse and discontinuous ...
... \r\n\r\nPublication No._____________\r\n\r\n\r\nMichele Lynn Marquette, Ph.D.\r\nThe University of Texas Medical Branch, 2007\r\n\r\nSupervisor: Marguerite A. Sognier\r\n\r\nThe objectives of this research were to: 1) develop a three-dimensional in vitro model; and 2) subsequently, utilize this model to investigate mechanisms of myoblast adhesion, fusion, and differentiation. C2C12 cells were examined as pre-aggregated single cells and multicellular aggregates in the Rotary Cell Culture System (RCCS). At the time intervals tested, RCCS cultured cells maintained viability and did not exhibit increased apoptosis markers such as Caspase 3 (activated) and phosphatydylserine. In contrast, increases in cell death and apoptotic markers were noted in suspension culture (SC) control cells. RCCS ...
Hippocampal culture and drug treatments. Embryonic day 18 (E18) rat hippocampi were cultured as described (Goslin and Banker, 1998). Cells were plated (90,000-120,000 cells per square centimeter) on poly-l-lysine-coated coverslips (1.0 mg/ml) in minimal essential medium (MEM) with FBS (10%) for 2 hr, inverted onto dishes containing astroglia, and grown in defined N2-conditioned medium (Goslin and Banker, 1998). Cells were fixed with 4% paraformaldehyde in 1× PBS at room temperature for 18 min or treated at various time points before fixation with 30 mm KCl (10 min, except where indicated), 50 μm APV (5 or 15 min; Tocris, Ellisville, MO), 100 μm CNQX (15 min; Tocris), or 1 mm (S)-α-methyl-4 carboxyphenylglycine (MCPG) (30 min; Tocris) added to KCl-containing media. Additionally, 10 μm 2-methyl-6-(phenylethynyl) pyridine hydrochloride (MPED) (Tocris) and 5 μm ...
human-diploid fibroblast cell cultures (strain WI-38)…fetal bovine serum…monosodium glutamate [MSG]…human serum albumin…magnesium stearate…FD&C Yellow #6 aluminum lake dye…acetone…castor oil…aluminum phosphate…formaldehyde…casein…inorganic salts…Fenton medium containing a bovine extract…aluminum hydroxide…polysorbate 80 (Tween 80)…VERO cells, a continuous line of monkey kidney cells…calf serum…neomycin sulfate…polymyxin B…ammonium sulfate…modified Mueller-Miller casamino acid medium without beef heart infusion…MRC-5 cells…normal human diploid cells…CMRL 1969 medium supplemented with calf serum…Medium 199 without calf serum…2-phenoxyethanol…synthetic medium…formalin…complex fermentation medium…soy peptone…mineral salts…potassium aluminum sulfate…amorphous aluminum hydroxyphosphate sulfate…sodium ...
The influence of the auxotrophic deficiencies of the host strain and expression vector selection on the production of a heterologous protein was investigated. Heterologous xylanase production by two prototrophic S. cerevisiae transformants, containing either a plasmid-based, YEp-type expression system or an integrative, YIp-type expression system, were compared with production by an auxotrophic transformant, containing an identical YEp-type expression system, in batch and continuous cultivation, using a chemically defined medium. Heterologous xylanase production by the auxotrophic strains in defined medium was critically dependent on the availability of amino acids, as extracellular xylanase production increased dramatically when amino acids were over-consumed from the medium to the point of saturating the cell. Saturation with amino acids, indicated by an increased leakage of amino acids from the cell, was thus a prerequisite for high level of heterologous; ...
article{7fb96577-46ba-4de6-8791-54c72ea71182, abstract = {Four qualitative methods for the detection of beta-lactamase production in Staphylococcus and Micrococcus species were evaluated and compared with a quantitative macroiodometric reference method. The disc diffusion test with penicillin G and the cloverleaf method could not separate beta-lactamase-positive from beta-lactamase-negative strains. Two applications of the chromogenic cephalosporin test, using uninduced strains and strains grown on blood agar plates, gave a large number of false negative and false positive results. False negative reactions were most common among uninduced strains, while the false positive reactions were most often recorded for Staphylococcus saprophyticus. A high degree of efficiency was recorded for the nitrocefin spot test, using induced strains grown on antibiotic susceptibility agar, and for the starch-iodine plate method. The starch-iodine plate with methicillin as inducer gave the most reliable results.}, ...
Sciatic Nerve Stuart, Chiropractic Adjustment Stuart, Chiropractor Near Me Stuart, Stenosis Stuart, Chiropractic Treatment Stuart, Chiropractic Care Stuart, Chiropractor Massage Stuart, Sciatica Pain Relief Stuart, Sciatic Nerve Pain Stuart, Sciatica Symptoms Stuart, Chiropractic Stuart, Chiropractic Clinic Stuart, Back Pain Stuart, Lower Back Pain Stuart, Spinal Stenosis Stuart, Pediatric Chiropractor Stuart, Neck Pain Stuart, Chiropracter Stuart, Advanced Chiropractic Stuart, Chiropractor Stuart, Pinched Nerve Stuart, Family Chiropractic Stuart, Sciatica Stuart, Stiff Neck Stuart, Best Chiropractor Stuart, Herniated Disc Stuart, Slipped Disc Stuart
Incubation of human erythrocytes in medium containing inosine (10 mM), pyruvate (10 mM), phosphate (50 mM) and NaCl (75 mM) at pH 6.6 leads to a more than 1000-fold increase in the concentration of 5-phosphoribosyl 1-pyrophosphate (PRPP), as identified and quantified by 31P-n.m.r. spectroscopy. The accumulation is highly pH-dependent, with a maximum at extracellular pH 6.60, and the maximum value of 1.3-1.6 mmol/l of erythrocytes is attained within 1 h at 37 degrees C. PRPP was accumulated despite high concentrations of 2,3-bisphosphoglycerate (2,3-BPG), an inhibitor of PRPP synthetase. The concentration of PRPP correlated with the intracellular concentration of inorganic phosphate (Pi). Substitution of either adenosine or adenosine plus inosine for inosine in the medium did not lead to 31P-n.m.r.-detectable accumulation of PRPP. These results show that neither 2,3-BPG nor PRPP itself inhibits the synthesis of PRPP in the human erythrocyte. Adenosine, however, prevents the ...
Concentrated clinical specimens are cultured using two different media types. The section utilizes the automated Mycobacteria Growth Indicator Tube (MGIT) system and Lowenstein-Jensen slants for the isolation and identification of mycobacteria. Positive isolates are identified by High Pressure Liquid Chromatography (HPLC) or DNA Probe. Isolates that are confirmed to contain Mycobacterium tuberculosis complex are considered critical and require same day submitter notification. Isolates that are confirmed to contain non-tuberculosis mycobacteria or actinomycetes are reported the same day but do not require submitter notification.. Cultures are incubated for a maximum of six weeks. A report of "no mycobacteria isolated" means that no acid-fast organisms have grown by the end of six weeks. A report of "contaminated" means one of the following: Initial smear positive and no growth on ...
Enhancement of the CAMP reaction by heated milk whey and maltose when incorporated into a selective agar medium, the thallium crystal violet toxin medium (TKT), is described. Milk whey was prepared by enzyme coagulation of milk at room temperature or by acid coagulation followed by neutralization with sodium hydroxide. Concentrations of whey from 1-14% (v/v) were examined. The CAMP reaction increased with increasing whey concentration. A similar increase in the CAMP reaction was also observed with increasing concentration of maltose up to 1% (w/v), beyond which it decreased with increasing concentration of maltose. A combination of heated milk whey with maltose produced a CAMP reaction in excess of that produced by whey or maltose alone ...
Of 130 strains classified as Bacillus subtilis, 60 fermented lactose and utilized gluconate slowly. High deoxyribonucleic acid relatedness values of 70 to 100% to the type strain (NRRL B-14393) of Bacillus amyloliquefaciens indicated these organisms to be strains of that species. The 70 remaining strains did not ferment lactose, utilized gluconate strongly, and were highly related genetically to the type strain (NRRL NRS-744) of B. subtilis. Lactose fermentation was observed in a standard medium containing 2% lactose instead of the usual 0.5%. Low deoxyribonucleic acid relatedness values of 25 to 37% established that neither group was related to B. pumilus, B. coagulans, B. firmus, or B. licheniformis. The results indicated that lactose fermentation and gluconate utilization are characteristics that can differentiate between B. subtilis and B. amyloliquefaciens.
The last 30 years Enterococcus faecium has become an important nosocomial pathogen in hospitals worldwide. The aim of this study was to obtain insight in the cell surface proteome of E. faecium when grown in laboratory and clinically relevant conditions. Enterococcus faecium E1162, a clinical blood stream isolate, was grown ... read more until mid-log phase in brain heart infusion medium (BHI) with, or without 0.02% bile salts, Tryptic Soy Broth with 1% glucose (TSBg) and urine, and its cell surface was "shaved" using immobilized trypsin. Peptides were identified using MS/MS. Mapping against the translated E1162 whole genome sequence identified 67 proteins that were differentially detected in different conditions. In urine, 14 proteins were significantly more and nine proteins less abundant relative to the other conditions. Growth in BHI-bile and TSBg, revealed four and six proteins, respectively, which were uniquely present in these conditions while two proteins were ...
Social Media for Business is an insiders guide to online marketing for the small business owner, manager or entrepreneur who wants to build the right multidimensional Web presence. Guaranteed to boost your social networking IQ, this book invests in your future with tips for sustainable tactics and savvy communications that are proven to deliver the real social media ROI - higher levels of customer engagement. Co-authors Martin Brossman and Anora McGaha speak from experience in educating small businesses and solo-professionals in the everyday strategies that drive social media results. In all, more than twenty experts contribute their perspectives on Web marketing and social media management. Social Media for Business: - Explains the competitive advantage for small and micro-business owners - Clarifies how businesses attract, engage and ...
Get human primary cells derived from normal and diseased tissue. Replicate the in vivo environment as closely as possible for more relevant results.
Get human primary cells derived from normal and diseased tissue. Replicate the in vivo environment as closely as possible for more relevant results.
Microbiology culture preparation. Person using a bunsen flame to sterilise the top of a jar containing a bacterial culture. - Stock Image C024/5723
We offer 4 types of normal human primary cells in the adipose cell system including endothelial cells, preadipocytes and mesenchymal stem cells.
In present investigation, B. subtilis and B. cereus was isolated and Identified from the petroleum contaminated site soil and identified by grams staining, motility and biochemical tests. After the isolation and identification, biosurfactant production from bacterial organisms was screened using mineral salt medium, by oil spreading, drop collapse and hemolysis test. From the blood agar plates, the biosurfactant producing bacterial culture were obtained and maintained as stock cultures. Organisms were inoculated into the mineral salt broth with petroleum oil, diesel oil, kerosene oil and crude oil as a carbon source for biosurfactant production. Biosurfactant was extracted by acid precipitation method. Biosurfactants production was optimized by using different pH, temperature, carbon and nitrogen sources. Emulsification activity of biosurfactant was analyzed. The extracted biosurfactant was characterized by using Thin Layer Chromatography ...
Salmonella enterica bacterial culture on XLD-Agar, which is a selective culture medium used in the isolation of Salmonella and Shigella species. Salmonella colonies typically grow colourless with a black centre. Salmonella enterica can cause gastroenteritis, bacteraemia, or enteric fever, but may be asymptomatic. - Stock Image C029/1360
The formation of viable but nonculturable (VBNC) Escherichia coli O157:H7 induced by high-pressure CO2 (HPCD) was investigated using RNA sequencing (RNA-Seq) transcriptomics and isobaric tag for relative and absolute quantitation (iTRAQ) proteomic methods. The analyses revealed that 97 genes and 56 proteins were significantly changed upon VBNC state entry. Genes and proteins related to membrane transport, central metabolisms, DNA replication, and cell division were mainly downregulated in the VBNC cells. This caused low metabolic activity concurrently with a division arrest in cells, which may be related to VBNC state formation. Cell division repression and outer membrane overexpression were confirmed to be involved in VBNC state formation by homologous expression of z2046 coding for transcriptional repressor and ompF encoding outer membrane protein F. Upon VBNC state entry, pyruvate catabolism in the cells shifted from the ...
The use of mature embryos as explants to initiate cultures is a best alternative to save time and costs, especially for producing somatic embryos for genetic transformation of durum wheat. However, plantlets regeneration from cultures derived from matured embryos is usually low. In this study, we tested matured embryos as explants from eight Moroccan durum wheat varieties (Irden, Marzak, Kyperounda, Isly, Amria, Karim, Marouane and Tomouh) to define suitable culture media for obtaining high frequencies of somatic embryogenesis and in vitro plantlets regeneration. For this purpose, we tested five induction and maintenance media (M1 to M5) based on MS media (macro and oligo-elements) which differed with respect to concentrations of plant hormones (2,4-D and BA), vitamins, sucrose, maltose, L-asparagine, and ...
Spawning market squid lay embryo capsules on the seafloor of the continental shelf of the California Current System (CCS), where ocean acidification, deoxygenation and intensified upwelling lower the pH and [O2]. Squid statolith geochemistry has been shown to reflect the squids environment (e.g., seawater temperature and elemental concentration). We used real-world environmental levels of pH and [O2] observed on squid-embryo beds to test in the laboratory whether or not squid statolith geochemistry reflects environmental pH and [O2]. We asked whether pH and [O2] levels might affect the incorporation of element ratios (B:Ca, Mg:Ca, Sr:Ca, Ba:Ca, Pb:Ca, U:Ca) into squid embryonic statoliths as (1) individual elements and/or (2) multivariate elemental signatures, and consider future applications as proxies for pH and [O2] exposure. Embryo exposure to high and low pH and [O2] alone and together during development over four weeks only moderately affected elemental concentrations of the statoliths, and
Production of lysine by Corynebacterium glutamicum (PTCC 1532) from different agricultural by-products (molasses and pulpy waste date) was compared to glucose as raw materials. For this purpose, ammonium sulphate was selected as a constant nitrogen source. The effect of different nitrogen sources was also investigated with glucose as a constant carbon source. The production of L-lysine was examined qualitatively and quantitatively using thin layer chromatography (TLC). Results of fermentation experiments showed that the maximum yield corresponded to molasses (48 g L-1) for the fermentation period of 96 hours. For other substrates the yield was lower and the period of fermentation exceeded that for molasses.
NIES-Collection (Microbial Culture Collection at the National Institute for Environmental Studies) was founded in 1983 within NIES. When the NIES-Collection was created, environmental issues such as eutrophication of lakes and rivers, air and water pollution, and human health problems caused by environmental pollution were much more severe than nowadays. The NIES-Collection started with ca. 250 strains mostly deposited by NIES scientists who were involved in environmental research and by other culture collections in Japan. Thus, red-tide-forming algae, such as Chattonella antiqua and Heterosigma akashiwo, and water-bloom-forming cyanobacteria, such as Microcystis aeruginosa were representatives at the start, and still characterize the culture collection ...
Growth Requirements. The culture media used for cell cultures are generally quite complex, and culture condition widely varies for each cell type. However, media generally include amino acids, vitamins, salts (maintain osmotic pressure), glucose, a bicarbonate buffer system (maintains a pH between 7.2 and 7.4), growth factors, hormones, O2 and CO2. To obtain best growth, addition of a small amount of blood serum is usually necessary, and several antibiotics, like penicillin and streptomycin are added to prevent bacterial contamination.. Temperature varies on the type of host cell. Most mammalian cells are maintained at 37oC for optimal growth, while cells derived from cold- blooded animals tolerate a wider temperature range (i.e. 15oC to ...
Aims: To analyse the relative importance of different biological and technical factors on the analytical sensitivity of conventional culture methods for detection of Salmonella Dublin in cattle faeces. Methods and Results: Faeces samples collected from six adult bovines from different salmonella-negative herds were split into subpools and spiked with three strains of S. Dublin at a concentration level of c. 10 CFU g(-1) faeces. Each of the 18 strain-pools was divided into two sets of triplicates of four volumes of faecal matter (1, 5, 10 and 25 g). The two sets were pre-enriched with and without novobiocin, followed by combinations of culture media (three types) and selective media (two types). The sensitivity of each combination and sources of variation in detection were determined by a generalized linear mixed model using a split-plot design. Conclusions: Biological ...
Complete article is available online.. PDF version is available online.. Trueperella abortisuis is a gram-positive bacterium that has been previously identified in aborted porcine feti and placentae located in Asia and Europe. Routine microbiological screening of extended boar semen from a US mid-Atlantic stud identified delayed growth of very small white colonies on both brain-heart infusion and sheep-blood agars after 48 to 72 hours incubation at 37°C under aerobic conditions. Isolate identification was performed using matrix-assisted laser desorption-ionization time of flight (MALDI-TOF) mass spectrometry, with T abortisuis identified (MALDI biotyper score = 2.103). After storage at 16°C and 72 hours post collection, total and progressive motility parameters had decreased in extended semen samples positive for T abortisuis. Further work is needed to elucidate the role T abortisuis may play in extended boar semen quality, extended semen longevity, and sow reproductive performance.. Keywords: ...
Liver mitochondria isolated from glucagon-treated rats by using both mannitol- and sucrose-based media showed enhanced uncoupled succinate oxidation, pyruvate metabolism and citrulline synthesis. Mitochondria prepared in mannitol medium showed some stimulation of these parameters compared with those prepared in sucrose medium. This was accompanied by an increase in matrix volume of about 20%. Some [14C]mannitol became permanently associated with mitochondria during preparation. It is suggested that mannitol may enter mitochondria during their preparation and cause swelling. The presence of 4mM-phosphate in the sucrose isolation medium stimulated the same parameters as did glucagon treatment, and also caused an increase in matrix volume of about 20%. These results confirm the conclusion that the mitochondrial volume may be important in the regulation of mitochondrial metabolism. They contradict the conclusion of others [Siess (1983) Hoppe-Seylers Z. Physiol. Chem. 364, ...
This is an immortalized cell line derived from the brain of an apparently normal 10 day old mouse. ref Cells were cloned in soft agar in the presence of CSF-1 and expanded on microcarrier beads. Beads were transferred to culture dishes and were subsequently passaged by scraping. 

Corning Announces Expanded Portfolio of Serum-free Cell Culture MediaCorning Announces Expanded Portfolio of Serum-free Cell Culture Media

... s serum-free product portfolio with the addition of two new cell culture media. ... Corning Announces Expanded Portfolio of Serum-free Cell Culture Media Corning Announces Expanded Portfolio of Serum-free Cell ... Corning Announces Expanded Portfolio of Serum-free Cell Culture Media. Corning Announces Expanded Portfolio of Serum-free. ... Corning Announces Expanded Portfolio of Serum-free Cell Culture Media. We use cookies to ensure the best experience on our ...
more infohttps://www.corning.com/cala/pt/products/life-sciences/news-events/news-releases/2012/10/lifesciences_us_canada_en_news_and_events_news_releases_2012_2012101001.html

Corning Announces Expanded Portfolio of Serum-free Cell Culture MediaCorning Announces Expanded Portfolio of Serum-free Cell Culture Media

... s serum-free product portfolio with the addition of two new cell culture media. ... Corning Announces Expanded Portfolio of Serum-free Cell Culture Media Corning Announces Expanded Portfolio of Serum-free Cell ... Corning Announces Expanded Portfolio of Serum-free Cell Culture Media. Corning Announces Expanded Portfolio of Serum-free. ... Corning Announces Expanded Portfolio of Serum-free Cell Culture Media. We use cookies to ensure the best experience on our ...
more infohttps://www.corning.com/emea/de/products/life-sciences/news-events/news-releases/2012/10/lifesciences_us_canada_en_news_and_events_news_releases_2012_2012101001.html

Lonza to Host New Webinar - Cell Culture Media: Why Go Serum-Free?Lonza to Host New Webinar - "Cell Culture Media: Why Go Serum-Free?"

... more and more scientists are considering serum-free cell culturing. In the webinar titled "Cell culture media: Why go serum- ... Free Lonza webinar to discuss the benefits of working with serum-free cell culture media and provide guidance on how to get the ... Lonza to Host New Webinar - "Cell Culture Media: Why Go Serum-Free?" October 01,2019 *News Release PDF ... In cell biology, cells are commonly grown in medium supplemented with serum. Serum is a highly variable, undefined product, ...
more infohttps://www.lonza.com/about-lonza/media-center/news/Tensid/2019-10-01-13-00-English.aspx

Keratinocyte cultures with serum or serum free media - Cell BiologyKeratinocyte cultures with serum or serum free media - Cell Biology

Top : Forum Archives: : Cell Biology. Keratinocyte cultures with serum or serum free media - (May/17/2005 ). can i know ... should it be cultivated under serum contained media or serum free media??. around what are the seeding densities do vary with ... should it be cultivated under serum contained media or serum free media??. around what are the seeding densities do vary with ... In my experience with serum-free media, cell lines that were normally adherent did not adhere, and did not grow well (Cho1.1, ...
more infohttp://www.protocol-online.org/biology-forums/posts/6738.html

IJMS | Free Full-Text | Comparison of Human Dermal Fibroblasts and HaCat Cells Cultured in Medium with or without Serum via a...IJMS | Free Full-Text | Comparison of Human Dermal Fibroblasts and HaCat Cells Cultured in Medium with or without Serum via a...

However, both cell types were successfully co-cultured in 2D using serum-free medium if the initial cell seeding density was ... Human dermal fibroblasts (HDFs) and immortalized keratinocytes (HaCat cells) mono- or co-cultured in serum or serum-free medium ... When mono-cultured on TCP, both HDFs and HaCat cells were less proliferative in medium without serum than with serum. ... research platform had great potential for in-depth understanding of HDFs and HaCat cells cultivated in serum-free medium, which ...
more infohttp://www.mdpi.com/1422-0067/19/2/388

Cell / Tissue Culture > Tissue Culture > Media, Serum Free  Laboratory Product Directory and Equipment Reviews on...Cell / Tissue Culture > Tissue Culture > Media, Serum Free Laboratory Product Directory and Equipment Reviews on...

Tissue Culture > Tissue Culture > Media, Serum Free products in the SelectScience products and suppliers directory ... Defined, xeno-free, serum-free culture medium, Designed to support the isolation and expansion of human mesenchymal stem cells ... Serum-Free Cell Freezing Medium. Biological Industries. A chemically defined, animal component-free, protein-free ... Defined, xeno-free, serum-free medium for long-term expansion of large and small vessels of endothelial cells. To be used with ...
more infohttp://www.selectscience.net/cell-+-tissue-culture/product-directory/media,-serum-free/?catID=1716&u=282661D4-C0B7-4038-8CCA-2932337AB5A8

US5945337A - Method for culturing CD34+ cells in a serum-free medium 
        - Google PatentsUS5945337A - Method for culturing CD34+ cells in a serum-free medium - Google Patents

The recipe for the formulation is given, which provides a medium suitable for the proliferation and differentiation of CD34 + ... cells purified from normal bone marrow, peripheral blood of patients treated with cytokines, and umbilical cord blood is ... A serum-free medium which supports the proliferation and differentiation of CD34 + ... Serum Free 2 951 Medium 1 Serum Free 2 60 Medium 2 IMDM + 20% 2 78 FBSExpt. Serum Free 2 1402 Medium 1 Serum Free 2 36 Medium 2 ...
more infohttps://patents.google.com/patent/US5945337

CellGenix GMP SCGM • serum-free culture media for HSCs & NK cellsCellGenix GMP SCGM • serum-free culture media for HSCs & NK cells

CellGenix GMP SCGM medium can be used for serum-free cultivation of HSCs as well as expansion and differentiation of NK & CIK ... Stem Cell Growth Medium. CellGenix GMP SCGM is an optimized, xeno-free medium used for the serum-free expansion of low numbers ... Hematopoietic stem cells (HSC). Cytokine induced killer cells (CIK cells). Natural killer cells (NK cells). ... Cytokines & Growth Factors Serum-free Media Bioprocessing Containers Supplements & Cells CellGenix® GMP SCGM. ...
more infohttps://cellgenix.com/products/gmp-scgm/

NutriStem® hPSC XF Medium A Superior Xeno-free, Serum-free Culture Medium for hES and hiPS CellsNutriStem® hPSC XF Medium A Superior Xeno-free, Serum-free Culture Medium for hES and hiPS Cells

... Featured Product Nov 21, 2016 ... Expanding the culture of excellence. NutriStem® hPSC XF Medium is a defined, xeno-free, serum-free medium designed to support ... cells in a feeder-free environment. NutriStem® hPSC XF Medium offers the ability to culture human pluripotent cells without the ... hPSC XF Medium and the leading competing medium for feeder-free culture. Medium was changed and proliferation was assessed ...
more infohttps://www.technologynetworks.com/drug-discovery/products/nutristem-hpsc-xf-medium-a-superior-xenofree-serumfree-culture-medium-for-hes-and-hips-cells-224909

Media Matters: Serum-Free Hematopoietic Cell Culture for Gene Editing and Gene Therapy Research | RegMedNetMedia Matters: Serum-Free Hematopoietic Cell Culture for Gene Editing and Gene Therapy Research | RegMedNet

Culturing HSPCs in Serum-Free Media for Cell Therapy Research Whether a protocol uses cell culture methods to expand edited ... Media Matters: Serum-Free Hematopoietic Cell Culture for Gene Editing and Gene Therapy Research. STEMCELL™ Technologies ... As compared to other serum-free media, StemSpan™ SFEM II medium supports the superior expansion of CD34+ cells when combined ... serum-free medium undergoes rigorous QC testing to ensure consistent conditions for the culture of HSPCs. StemSpan™ media do ...
more infohttps://www.regmednet.com/posts/20399-media-matters-serum-free-hematopoietic-cell-culture-for-gene-editing-and-gene-therapy-research

Isolation of tumor - secreted products from human carcinoma cells maintained in a defined protein - free medium ( serum - free...Isolation of tumor - secreted products from human carcinoma cells maintained in a defined protein - free medium ( serum - free...

... free medium ( serum - free culture medium / conditioned medium / tumor antigens / growth factors / glutamine ) by Edward M. ... secreted products from human carcinoma cells maintained in a defined protein - ... free medium ( serum - free culture medium / conditioned medium / tumor antigens / growth factors / glutamine ). *. Edward M. ... free medium ( serum - free culture medium / conditioned medium / tumor antigens / growth factors / glutamine )}, author={Edward ...
more infohttps://www.semanticscholar.org/paper/Isolation-of-tumor---secreted-products-from-human-Alderman-Lobb/6884c5dc6995f06aced23d59f964e3be69d11549

NAVER Academic | Growth and differentation of rat mammary epithelial cells cultured in serum-free mediumNAVER Academic | Growth and differentation of rat mammary epithelial cells cultured in serum-free medium

... complete hormone serum-free medium, CHSFM) showed cell-cell communication at 14 days of primary culture or of subculture. By ... basal serum-free medium, BSFM) failed to show cell-cell communication. However, colonies cultured in BSFM supplemented with ... Mammary organoids from the glands of female F344 rats were cultured in a serum-free medium. Monolayer culture colonies ... we distinguished four RMEC subpopulations in cultures in both media: Thy-1.1+ cells, PNA+ cells, cells negative to both ...
more infohttps://academic.naver.com/article.naver?doc_id=141269661

Cell Culture Media | Fisher ScientificCell Culture Media | Fisher Scientific

Sf9 Serum-free/Protein-free Medium, 1X, With L-glutamine Formulated to support propagation of Sf9 insect cells in culture. ... HyClone™ SFX Insect™ Cell Culture Media is a protein-free media that support cell culture growth. ... Cell Culture & Analysis Cell Culture & Analysis * Cell Culture Dishes, Plates and Flasks ... CDM4PERMAb cell culture media. Chemically defined Animal-Derived Component-Free (ADCF) media for use with PER.C6 cells ...
more infohttps://www.fishersci.com/us/en/products/life-sciences/cell-culture/cell-culture-media.html

Riboflavin in Cell Culture | China-Mainland | Sigma-AldrichRiboflavin in Cell Culture | China-Mainland | Sigma-Aldrich

... protein-free and animal protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal ... This page segues to comprehensive insights on how riboflavin and other important cell culture components affect the performance ... Sigmas Cell Culture Media Expert provides in depth discussion of this and other serum-free and protein-free media supplements ... used as a starting formulation for development of proprietary serum-free, or protein-free cell culture media for Chinese ...
more infohttps://www.sigmaaldrich.com/china-mainland/zh/life-science/cell-culture/learning-center/media-expert/riboflavin.html

Selenium in Cell Culture | Sigma-AldrichSelenium in Cell Culture | Sigma-Aldrich

... protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page ... How the trace metal selenium and other cell culture components affect the performance of serum-free, ... Our Cell Culture Media Expert provides in depth discussion of this and other serum-free and protein-free media supplements. The ... Serum-Free/Protein Free Hybridoma Medium; Swims S-77 Medium; Waymouth Medium MB; Williams Medium E and various proprietary ...
more infohttps://www.sigmaaldrich.com/life-science/cell-culture/learning-center/media-expert/selenium.html

Animal Cell Culture Techniques | SpringerLinkAnimal Cell Culture Techniques | SpringerLink

Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological ... Serum-Free Media Joanne Keenan, Paula Meleady, Martin Clynes. Pages 54-66 ... Colon Monoclonal Antibodies cell cell culture cell cycle cell differentiation cells cytokine gene expression gene therapy ... The Human Leukemia Cell Line HL-60 as a Cell Culture Model To Study Neutrophil Functions and Inflammatory Cell Responses ...
more infohttps://link.springer.com/book/10.1007%2F978-3-642-80412-0

Team:Freiburg Bioware/Project/Methods - 2010.igem.orgTeam:Freiburg Bioware/Project/Methods - 2010.igem.org

Serum-free cell culture medium. Introduction. Serum-free mediums allow users to standardize their cell culture conditions. It ... Testing serum free medium. Serum free medium was obtained from AAV-293 cells are not adapted to serum-free growth conditions so ... serum-free medium, e.g. 15 ml DMEM (not serum-free) + 5 ml serum-free medium (FreeStyle™ 293 Expression Medium) for a T75 flask ... We raised the serum-free ratio to 100 % over 7 passages.. 100% serum-free cells grow slower compared to the serum-supplemented ...
more infohttp://2010.igem.org/wiki/index.php?title=Team:Freiburg_Bioware/Project/Methods&diff=prev&oldid=190898

Team:Freiburg Bioware/Project/Methods - 2010.igem.orgTeam:Freiburg Bioware/Project/Methods - 2010.igem.org

Serum-free cell culture medium. Introduction. Serum-free mediums allow users to standardize their cell culture conditions. It ... Testing serum free medium. Serum free medium was obtained from AAV-293 cells are not adapted to serum-free growth conditions so ... serum-free medium, e.g. 15 ml DMEM (not serum-free) + 5 ml serum-free medium (FreeStyle™ 293 Expression Medium) for a T75 flask ... We raised the serum-free ratio to 100 % over 7 passages.. 100% serum-free cells grow slower compared to the serum-supplemented ...
more infohttp://2010.igem.org/wiki/index.php?title=Team:Freiburg_Bioware/Project/Methods&diff=prev&oldid=190846

Buy Media formulationsBuy Media formulations

... have been engineered to provide the basic media nutrients required for the growth and support of several specific cell types ... PeproGrow-1 Serum-Free Cell Culture Supplement Kit. Catalog Number: 700-C100 ... Media Products. Aiming to support your cell culture needs, our Media and Media Supplement products provide you with the ... components necessary for the successful culturing and preservation of several cell types.. ...
more infohttps://www.peprotech.com/gb/media-products

Buy Media formulationsBuy Media formulations

... have been engineered to provide the basic media nutrients required for the growth and support of several specific cell types ... PeproGrow-1 Serum-Free Cell Culture Supplement Kit. Numero de catalogo: 700-C100 ... Media Products. Aiming to support your cell culture needs, our Media and Media Supplement products provide you with the ... components necessary for the successful culturing and preservation of several cell types.. ...
more infohttps://www.peprotech.com/es/media-products

Patent US6440930 - Protein formulations - Google PatentsPatent US6440930 - Protein formulations - Google Patents

Serum free cell culture medium. US5118666. Jun 1, 1990. Jun 2, 1992. The General Hospital Corporation. Insulinotropic hormone. ... Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:RINELLA, JOSEPH VINCENT, JR.;REEL/FRAME:011736/0793 ... DNA encoding the beta subunit of human follide stimulating hormone and expression vectors and cells containing same. ... making possible a multi-use parenteral formulation that is relatively free of protein aggregation. ...
more infohttp://www.google.com/patents/US6440930?dq=6,123,819

Stem Cells:  Drug Discovery and Therapeutics : Pharmaceuticals : AllStem Cells: Drug Discovery and Therapeutics : Pharmaceuticals : All

Serum free culture media. *Starting material. *Reproducible end product. 10:55 Morning Coffee. ... 10:20 STANDARDIZATION OF STEM CELL CULTURING. Judit Nagy, Research Scientist, Director Proteomics Facility, Imperial College. * ... 9:45 HOW TO BOOST STEM CELL NUMBERS IN THE BLOOD. Sara Rankin, Reader in Leukocyte and Stem Cell Biology, National Heart & Lung ... 15:25 STEM CELLS IN PREDICTIVE TOXICOLOGY. Louise Leong, Science Director, SC4SM, Stem Cells for Safer Medicine. *SC4SM - A ...
more infohttp://www.smi-online.co.uk/pharmaceuticals/archive/2-2009/conference/stem-cells/

Patent US5405772 - Medium for long-term proliferation and development of cells - Google PatentsPatent US5405772 - Medium for long-term proliferation and development of cells - Google Patents

... a standard culture medium such as Iscoves modified Dulbeccos medium; serum albumin; transferrin; a source of lipids and fatty ... particularly hematopoietic cells and bone marrow stromal cells, the medium comprising cell proliferation and development ... when the cells to be cultured are hematopoietic cells); nucleosides for synthesis of DNA and RNA; growth factors that stimulate ... the proliferation and development of stromal cells and cells from a variety of tissues or organs, such as epidermal growth ...
more infohttp://www.google.com/patents/US5405772?dq=U.S.+Patent+

STEMPRO® MSC SFM-Human Mesenchymal Stem Cell Culture Medium | Thermo Fisher Scientific - USSTEMPRO® MSC SFM-Human Mesenchymal Stem Cell Culture Medium | Thermo Fisher Scientific - US

... specially formulated for the growth and expansion of human mesenchymal stem cells (hMSCs) ... First serum-free medium for hMSCs * Superior efficiency of hMSC expansion at high cell densities-less medium, surface area, and ... the first serum-free medium (SFM) specially formulated for the growth and expansion of human mesenchymal stem cells (hMSCs) ... CELLstart™ - Substrate for serum-free hMSC culture CELLstart™ is designed for use with STEMPRO® MSC SFM as a substrate to ...
more infohttps://www.thermofisher.com/us/en/home/life-science/stem-cell-research/stem-cell-culture/stem-cell-research-misc/stempro-msc-sfm.html

Retracted] Serum‑free‑medium‑type mesenchymal stem cell culture supernatant exerts a protective effect on A549 lung epithelial...Retracted] Serumfreemedium‑type mesenchymal stem cell culture supernatant exerts a protective effect on A549 lung epithelial...

Serum-free-medium-type mesenchymal stem cell culture supernatant exerts a protective effect on A549 lung epithelial cells in ... Retracted] Serumfreemedium‑type mesenchymal stem cell culture supernatant exerts a protective effect on A549 lung epithelial ... Wu, J., Shang, A., Chen, C., Wang, W., Xiong, C., Guo, N.[Retracted] Serumfreemedium‑type mesenchymal stem cell culture ... Wu, J., Shang, A., Chen, C., Wang, W., Xiong, C., Guo, N.[Retracted] Serumfreemedium‑type mesenchymal stem cell culture ...
more infohttps://tessera.spandidos-publications.com/or/41/6/3586?text=fulltext
  • Hematopoietic stem cells (HSCs) have long been used to provide lifesaving treatments for patients with malignant and non-malignant hematological disorders. (regmednet.com)
  • Lentiviral-based gene therapy methods to modify human CD34+ hematopoietic stem cells have been investigated as a way of treating various hematological disorders including X-linked chronic granulomatous disease (X-CGD). (regmednet.com)
  • The use of FCS to supplement cell culture medium for AAV particle production is problematic because even smallest amounts of animal antigens in the administered drug could lead to a strong immune response in patients. (igem.org)
  • Aiming to support your cell culture needs, our Media and Media Supplement products provide you with the components necessary for the successful culturing and preservation of several cell types. (peprotech.com)
  • Stem cells in numerous tissues replace mature cell loss during physical activity or injury throughout life. (biomedcentral.com)
  • Controlling the behavior of your stem cells to ensure stemness or to efficiently stimulate differentiation along a particular lineage can be challenging. (clontech.com)
  • Cell biomass was estimated by protein assay using Lowry's method. (uclouvain.be)
  • citation needed] Using a standard Raji assay employing 125I-IgG goat anti-human Fc gamma, the digestion of Raji cells with pronase reduced by 95% their ability to bind complement-fixed aggregated human gamma globulin and complement-fixed tetanus toxoid-antitetanus toxin complexes. (wikipedia.org)
  • CellGenix GMP SCGM is widely used for standardized generation of autologous and allogeneic cell therapies using HSCs, NK cells or CIK cells. (cellgenix.com)
  • While originally these treatments took the form of allogeneic bone marrow transplants, more recent breakthroughs in the field have provided cell therapy researchers with methods for correcting genetic mutations in a patient's own cells to provide an autologous source of HSCs for transplant, or to expand cells ex vivo and extend the potential of allogeneic cell sources. (regmednet.com)
  • Corning Incorporated (NYSE: GLW) today announced the expansion of Mediatech, Inc.'s serum-free product portfolio with the addition of two new cell culture media. (corning.com)
  • The Corning ® Hepatocyte Maintenance Medium and the Corning stemgro ® MSC Medium capitalize on the growing global demand for specifically defined, serum-free media. (corning.com)
  • Regulatory requirements, performance, and cost concerns have increasingly led to the development of serum-free media alternatives and their use by researchers worldwide," said Jason Walsh, business director, Mediatech, Corning Life Sciences . (corning.com)
  • Corning™ Minimum Essential Medium Eagle (Mod. (fishersci.com)
  • SeP may serve as the primary selenium transport and delivery protein to cells especially neuronal and immune cells. (sigmaaldrich.com)
  • Curiously, although α-Syn aggregation and pathology are prominent in humans and in animal models of synucleinopathies ( 8 - 10 ), overexpression of α-Syn in neuronal and nonneuronal cells, as well as primary neurons derived from α-Syn transgenic mice, does not lead to significant α-Syn inclusion formation ( 11 ). (pnas.org)
  • As compared to other serum-free media, StemSpan™ SFEM II medium supports the superior expansion of CD34+ cells when combined with cytokine supplements formulated specifically for this purpose (Figure 1). (regmednet.com)
  • They applied this method to modify HSCs isolated from patients with X-CGD and found that this restored the antimicrobial activity of neutrophils derived from the transgene-containing cell population. (regmednet.com)
  • On repeating certain of the experiments, the authors found that the Nrf2-Keap1-ARE signaling pathway only has a role in the lung epithelial cell injury model, whereas it does not serve a role in the A549 model. (spandidos-publications.com)
  • The pluripotent H9.2 cells were suspended in serum-supplemented medium, where they spontaneously formed EBs containing cells of embryonic germ layers. (technologynetworks.com)
  • Clontech offers a range of media and supplements for the derivation, maintenance, and expansion of embryonic stem (ES), induced pluripotent stem (iPS), embryonic germ (EG), and neural stem (NS) cells. (clontech.com)
  • Basel, Switzerland, 1 October 2019 - On 15 October, Lonza will host a free 60-minute webinar that will address current concerns regarding the use of serum in cell culture media, explore viable alternatives and provide guidance on how to adapt cells to a serum-free environment. (lonza.com)
  • The ability to scale up hMSC production will be critical for any viable cell therapy. (thermofisher.com)
  • The chemistry and bioavailability of riboflavin in cell culture plays an important role in the stability and utility of media used for biomanufacturing of heterologous proteins and tissue engineering. (sigmaaldrich.com)
  • The cells were used for AAV production, and we produced similar amounts of virus particles compared to cells grown in FCS-supplemented medium. (igem.org)
  • In this technique, cells are taken from an animal or plant and grown under favourable artificial conditions. (bizpr.us)
  • The following cell types were identified by examination of the histological sections of14-day-old EBs stained with H&E: (A) neural rosette (ectoderm), (B) neural rosette stained with Tubulin, (C) primitive blood vessels (mesoderm), and (D) megakaryocytes (mesoderm). (technologynetworks.com)
  • hMSCs cultured in STEMPRO® MSC SFM retained the tri-lineage mesoderm differentiation potential (adipocyte, chondrocyte, and osteoblast) beyond passage 5 (Figure 2). (thermofisher.com)
  • Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. (springer.com)
  • It was demonstrated that serum depletion had significant influence on the attachment of HaCat cells onto tissue culture plastic (TCP), porous substrates and cellulosic scaffolds, which was further enhanced by the pre-seeded HDFs. (mdpi.com)
  • The complex structures formed by the HDFs colonized within the porous substrates and scaffolds not only prevented the seeded HaCat cells from filtering through the open pores, but also acted as cellular substrates for HaCat cells to attach onto. (mdpi.com)
  • Based on the results from 2D cultures, co-culture of both cell types on modular substrates with small open pores (125 μm) and cellulosic scaffolds with open pores of varying sizes (50-300 µm) were then conducted successfully in serum-free medium. (mdpi.com)