Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.
The process of losing secretory granules (SECRETORY VESICLES). This occurs, for example, in mast cells, basophils, neutrophils, eosinophils, and platelets when secretory products are released from the granules by EXOCYTOSIS.
The secretion of histamine from mast cell and basophil granules by exocytosis. This can be initiated by a number of factors, all of which involve binding of IgE, cross-linked by antigen, to the mast cell or basophil's Fc receptors. Once released, histamine binds to a number of different target cell receptors and exerts a wide variety of effects.
A family of neutral serine proteases with TRYPSIN-like activity. Tryptases are primarily found in the SECRETORY GRANULES of MAST CELLS and are released during mast cell degranulation.
A family of neutral serine proteases with CHYMOTRYPSIN-like activity. Chymases are primarily found in the SECRETORY GRANULES of MAST CELLS and are released during mast cell degranulation.
Specific molecular sites on the surface of B- and T-lymphocytes which combine with IgEs. Two subclasses exist: low affinity receptors (Fc epsilon RII) and high affinity receptors (Fc epsilon RI).
A potent mast cell degranulator. It is involved in histamine release.
An immunoglobulin associated with MAST CELLS. Overexpression has been associated with allergic hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).
A heterogenous group of disorders characterized by the abnormal increase of MAST CELLS in only the skin (MASTOCYTOSIS, CUTANEOUS), in extracutaneous tissues involving multiple organs (MASTOCYTOSIS, SYSTEMIC), or in solid tumors (MASTOCYTOMA).
A chromone complex that acts by inhibiting the release of chemical mediators from sensitized mast cells. It is used in the prophylactic treatment of both allergic and exercise-induced asthma, but does not affect an established asthmatic attack.
A protein-tyrosine kinase receptor that is specific for STEM CELL FACTOR. This interaction is crucial for the development of hematopoietic, gonadal, and pigment stem cells. Genetic mutations that disrupt the expression of PROTO-ONCOGENE PROTEINS C-KIT are associated with PIEBALDISM, while overexpression or constitutive activation of the c-kit protein-tyrosine kinase is associated with tumorigenesis.
An amine derived by enzymatic decarboxylation of HISTIDINE. It is a powerful stimulant of gastric secretion, a constrictor of bronchial smooth muscle, a vasodilator, and also a centrally acting neurotransmitter.
An acute hypersensitivity reaction due to exposure to a previously encountered ANTIGEN. The reaction may include rapidly progressing URTICARIA, respiratory distress, vascular collapse, systemic SHOCK, and death.
A group of disorders caused by the abnormal proliferation of MAST CELLS in a variety of extracutaneous tissues including bone marrow, liver, spleen, lymph nodes, and gastrointestinal tract. Systemic mastocytosis is commonly seen in adults. These diseases are categorized on the basis of clinical features, pathologic findings, and prognosis.
Any member of the group of ENDOPEPTIDASES containing at the active site a serine residue involved in catalysis.
A hematopoietic growth factor and the ligand of the cell surface c-kit protein (PROTO-ONCOGENE PROTEINS C-KIT). It is expressed during embryogenesis and is a growth factor for a number of cell types including the MAST CELLS and the MELANOCYTES in addition to the HEMATOPOIETIC STEM CELLS.
The space enclosed by the peritoneum. It is divided into two portions, the greater sac and the lesser sac or omental bursa, which lies behind the STOMACH. The two sacs are connected by the foramen of Winslow, or epiploic foramen.
An evanescent cutaneous reaction occurring when antibody is injected into a local area on the skin and antigen is subsequently injected intravenously along with a dye. The dye makes the rapidly occurring capillary dilatation and increased vascular permeability readily visible by leakage into the reaction site. PCA is a sensitive reaction for detecting very small quantities of antibodies and is also a method for studying the mechanisms of immediate hypersensitivity.
A form of systemic mastocytosis (MASTOCYTOSIS, SYSTEMIC) characterized by the presence of large numbers of tissue MAST CELLS in the peripheral blood without skin lesions. It is a high-grade LEUKEMIA disease with bone marrow smear of >20% MAST CELLS, multi-organ failure and a short survival.
A hexosaminidase specific for non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. It acts on GLUCOSIDES; GALACTOSIDES; and several OLIGOSACCHARIDES. Two specific mammalian isoenzymes of beta-N-acetylhexoaminidase are referred to as HEXOSAMINIDASE A and HEXOSAMINIDASE B. Deficiency of the type A isoenzyme causes TAY-SACHS DISEASE, while deficiency of both A and B isozymes causes SANDHOFF DISEASE. The enzyme has also been used as a tumor marker to distinguish between malignant and benign disease.
Granular leukocytes characterized by a relatively pale-staining, lobate nucleus and cytoplasm containing coarse dark-staining granules of variable size and stainable by basic dyes.
Carboxypeptidases that are primarily found the DIGESTIVE SYSTEM that catalyze the release of C-terminal amino acids. Carboxypeptidases A have little or no activity for hydrolysis of C-terminal ASPARTIC ACID; GLUTAMIC ACID; ARGININE; LYSINE; or PROLINE. This enzyme requires ZINC as a cofactor and was formerly listed as EC 3.4.2.1 and EC 3.4.12.2.
A cycloheptathiophene blocker of histamine H1 receptors and release of inflammatory mediators. It has been proposed for the treatment of asthma, rhinitis, skin allergies, and anaphylaxis.
Condensed areas of cellular material that may be bounded by a membrane.
The number of CELLS of a specific kind, usually measured per unit volume or area of sample.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Compounds with three aromatic rings in linear arrangement with a SULFUR in the center ring.
The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
A unifocal malignant tumor that consists of atypical pathological MAST CELLS without systemic involvement. It causes local destructive growth in organs other than in skin or bone marrow.
A phenothiazine that has been used as a hemostatic, a biological stain, and a dye for wool and silk. Tolonium chloride has also been used as a diagnostic aid for oral and gastric neoplasms and in the identification of the parathyroid gland in thyroid surgery.
Altered reactivity to an antigen, which can result in pathologic reactions upon subsequent exposure to that particular antigen.
A solid tumor consisting of a dense infiltration of MAST CELLS. It is generally benign.
The most common form of cutaneous mastocytosis (MASTOCYTOSIS, CUTANEOUS) that occurs primarily in children. It is characterized by the multiple small reddish-brown pigmented pruritic macules and papules.
Agents that are used to treat allergic reactions. Most of these drugs act by preventing the release of inflammatory mediators or inhibiting the actions of released mediators on their target cells. (From AMA Drug Evaluations Annual, 1994, p475)
A group of cells that includes FIBROBLASTS, cartilage cells, ADIPOCYTES, smooth muscle cells, and bone cells.
A rare acute myeloid leukemia in which the primary differentiation is to BASOPHILS. It is characterized by an extreme increase of immature basophilic granulated cells in the bone marrow and blood. Mature basophils are usually sparse.
The principal cyclooxygenase metabolite of arachidonic acid. It is released upon activation of mast cells and is also synthesized by alveolar macrophages. Among its many biological actions, the most important are its bronchoconstrictor, platelet-activating-factor-inhibitory, and cytotoxic effects.
A multilineage cell growth factor secreted by LYMPHOCYTES; EPITHELIAL CELLS; and ASTROCYTES which stimulates clonal proliferation and differentiation of various types of blood and tissue cells.
Mice bearing mutant genes which are phenotypically expressed in the animals.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
The conjugation product of LEUKOTRIENE A4 and glutathione. It is the major arachidonic acid metabolite in macrophages and human mast cells as well as in antigen-sensitized lung tissue. It stimulates mucus secretion in the lung, and produces contractions of nonvascular and some VASCULAR SMOOTH MUSCLE. (From Dictionary of Prostaglandins and Related Compounds, 1990)
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
Drugs that selectively bind to but do not activate histamine H1 receptors, thereby blocking the actions of endogenous histamine. Included here are the classical antihistaminics that antagonize or prevent the action of histamine mainly in immediate hypersensitivity. They act in the bronchi, capillaries, and some other smooth muscles, and are used to prevent or allay motion sickness, seasonal rhinitis, and allergic dermatitis and to induce somnolence. The effects of blocking central nervous system H1 receptors are not as well understood.
An infection with TRICHINELLA. It is caused by eating raw or undercooked meat that is infected with larvae of nematode worms TRICHINELLA genus. All members of the TRICHINELLA genus can infect human in addition to TRICHINELLA SPIRALIS, the traditional etiological agent. It is distributed throughout much of the world and is re-emerging in some parts as a public health hazard and a food safety problem.
A family of biologically active compounds derived from arachidonic acid by oxidative metabolism through the 5-lipoxygenase pathway. They participate in host defense reactions and pathophysiological conditions such as immediate hypersensitivity and inflammation. They have potent actions on many essential organs and systems, including the cardiovascular, pulmonary, and central nervous system as well as the gastrointestinal tract and the immune system.
A genus of intestinal nematode parasites belonging to the superfamily HELIGMOSOMATOIDEA, which commonly occurs in rats but has been experimentally transmitted to other rodents and rabbits. Infection is usually through the skin.
A basic helix-loop-helix leucine zipper transcription factor that regulates the CELL DIFFERENTIATION and development of a variety of cell types including MELANOCYTES; OSTEOCLASTS; and RETINAL PIGMENT EPITHELIUM. Mutations in MITF protein have been associated with OSTEOPETROSIS and WAARDENBURG SYNDROME.
Cellular release of material within membrane-limited vesicles by fusion of the vesicles with the CELL MEMBRANE.
A soluble factor produced by activated T-LYMPHOCYTES that induces the expression of MHC CLASS II GENES and FC RECEPTORS on B-LYMPHOCYTES and causes their proliferation and differentiation. It also acts on T-lymphocytes, MAST CELLS, and several other hematopoietic lineage cells.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Granular leukocytes with a nucleus that usually has two lobes connected by a slender thread of chromatin, and cytoplasm containing coarse, round granules that are uniform in size and stainable by eosin.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
A multifunctional cytokine secreted by primarily by activated TH2 CELLS that may play a role as a regulator of allergic INFLAMMATION. It has been shown to enhance the growth and CELL DIFFERENTIATION of MAST CELLS, and can act on a variety of other immune cells.
An enzyme that catalyzes the decarboxylation of histidine to histamine and carbon dioxide. It requires pyridoxal phosphate in animal tissues, but not in microorganisms. EC 4.1.1.22.
A parasite of carnivorous mammals that causes TRICHINELLOSIS. It is especially common in rats and in swine fed uncooked garbage. Human infection is initiated by the consumption of raw or insufficiently cooked pork or other meat containing the encysted larvae.
Substances that are recognized by the immune system and induce an immune reaction.
Organic compounds that contain two nitro groups attached to a phenol.
A copper-containing dye used as a gelling agent for lubricants, for staining of bacteria and for the dyeing of histiocytes and fibroblasts in vivo.
Established cell cultures that have the potential to propagate indefinitely.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.
These growth factors comprise a family of hematopoietic regulators with biological specificities defined by their ability to support proliferation and differentiation of blood cells of different lineages. ERYTHROPOIETIN and the COLONY-STIMULATING FACTORS belong to this family. Some of these factors have been studied and used in the treatment of chemotherapy-induced neutropenia, myelodysplastic syndromes, and bone marrow failure syndromes.
Antigen-type substances that produce immediate hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).

Socs1 binds to multiple signalling proteins and suppresses steel factor-dependent proliferation. (1/5252)

We have identified Socs1 as a downstream component of the Kit receptor tyrosine kinase signalling pathway. We show that the expression of Socs1 mRNA is rapidly increased in primary bone marrow-derived mast cells following exposure to Steel factor, and Socs1 inducibly binds to the Kit receptor tyrosine kinase via its Src homology 2 (SH2) domain. Previous studies have shown that Socs1 suppresses cytokine-mediated differentiation in M1 cells inhibiting Janus family kinases. In contrast, constitutive expression of Socs1 suppresses the mitogenic potential of Kit while maintaining Steel factor-dependent cell survival signals. Unlike Janus kinases, Socs1 does not inhibit the catalytic activity of the Kit tyrosine kinase. In order to define the mechanism by which Socs1-mediated suppression of Kit-dependent mitogenesis occurs, we demonstrate that Socs1 binds to the signalling proteins Grb-2 and the Rho-family guanine nucleotide exchange factors Vav. We show that Grb2 binds Socs1 via its SH3 domains to putative diproline determinants located in the N-terminus of Socs1, and Socs1 binds to the N-terminal regulatory region of Vav. These data suggest that Socs1 is an inducible switch which modulates proliferative signals in favour of cell survival signals and functions as an adaptor protein in receptor tyrosine kinase signalling pathways.  (+info)

Borrelia burgdorferi spirochetes induce mast cell activation and cytokine release. (2/5252)

The Lyme disease spirochete, Borrelia burgdorferi, is introduced into human hosts via tick bites. Among the cell types present in the skin which may initially contact spirochetes are mast cells. Since spirochetes are known to activate a variety of cell types in vitro, we tested whether B. burgdorferi spirochetes could activate mast cells. We report here that freshly isolated rat peritoneal mast cells or mouse MC/9 mast cells cultured in vitro with live or freeze-thawed B. burgdorferi spirochetes undergo low but detectable degranulation, as measured by [5-3H] hydroxytryptamine release, and they synthesize and secrete the proinflammatory cytokine tumor necrosis factor alpha (TNF-alpha). In contrast to findings in previous studies, where B. burgdorferi-associated activity was shown to be dependent upon protein lipidation, mast cell TNF-alpha release was not induced by either lipidated or unlipidated recombinant OspA. This activity was additionally shown to be protease sensitive and surface expressed. Finally, comparisons of TNF-alpha-inducing activity in known low-, intermediate-, and high-passage B. burgdorferi B31 isolates demonstrated passage-dependent loss of activity, indicating that the activity is probably plasmid encoded. These findings document the presence in low-passage B. burgdorferi spirochetes of a novel lipidation-independent activity capable of inducing cytokine release from host cells.  (+info)

gp49B1 inhibits IgE-initiated mast cell activation through both immunoreceptor tyrosine-based inhibitory motifs, recruitment of src homology 2 domain-containing phosphatase-1, and suppression of early and late calcium mobilization. (3/5252)

We define by molecular, pharmacologic, and physiologic approaches the proximal mechanism by which the immunoglobulin superfamily member gp49B1 inhibits mast cell activation mediated by the high affinity Fc receptor for IgE (FcepsilonRI). In rat basophilic leukemia-2H3 cells expressing transfected mouse gp49B1, mutation of tyrosine to phenylalanine in either of the two immunoreceptor tyrosine-based inhibitory motifs of the gp49B1 cytoplasmic domain partially suppressed gp49B1-mediated inhibition of exocytosis, whereas mutation of both abolished inhibitory capacity. Sodium pervanadate elicited tyrosine phosphorylation of native gp49B1 and association of the tyrosine phosphatases src homology 2 domain-containing phosphatase-1 (SHP-1) and SHP-2 in mouse bone marrow-derived mast cells (mBMMCs). SHP-1 associated transiently with gp49B1 within 1 min after coligation of gp49B1 with cross-linked FcepsilonRI in mBMMCs. SHP-1-deficient mBMMCs exhibited a partial loss of gp49B1-mediated inhibition of FcepsilonRI-induced exocytosis at concentrations of IgE providing optimal exocytosis, revealing a central, but not exclusive, SHP-1 requirement in the counter-regulatory pathway. Coligation of gp49B1 with cross-linked FcepsilonRI on mBMMCs inhibited early release of calcium from intracellular stores and subsequent influx of extracellular calcium, consistent with SHP-1 participation. Because exocytosis is complete within 2 min in mBMMCs, our studies establish a role for SHP-1 in the initial counter-regulatory cellular responses whereby gp49B1 immunoreceptor tyrosine-based inhibition motifs rapidly transmit inhibition of FcepsilonRI-mediated exocytosis.  (+info)

Bone marrow angiogenesis and mast cell density increase simultaneously with progression of human multiple myeloma. (4/5252)

Immunohistochemical, cytochemical and ultrastructural data showing vivid angiogenesis and numerous mast cells (MCs) in the bone marrow of 24 patients with active multiple myeloma (MM) compared with 34 patients with non-active MM and 22 patients with monoclonal gammopathy of undetermined significance (MGUS) led us to hypothesize that angiogenesis parallels progression of MM, and that MCs participate in its induction via angiogenic factors in their secretory granules.  (+info)

Potent mast cell degranulation and vascular permeability triggered by urocortin through activation of corticotropin-releasing hormone receptors. (5/5252)

Urocortin (Ucn) is related to corticotropin-releasing hormone (CRH), and both are released in the brain under stress where they stimulate CRH 1 and 2 receptors (CRHR). Outside the brain, they may have proinflammatory actions through activation of mast cells, which are located perivascularly close to nerve endings and degranulate in response to acute psychological stress. Here, we report that a concentration of intradermal Ucn as low as 10 nM induced dose-dependent rat skin mast cell degranulation and increased vascular permeability. This effect appeared to be equipotent to that of calcitonin gene-related peptide and neurotensin. Ucn-induced skin vasodilation was inhibited by pretreatment with the mast cell stabilizer disodium cromoglycate (cromolyn) and was absent in the mast cell-deficient W/Wv mice. The selective nonpeptide CRH receptor 1 antagonist, antalarmin and the nonselective peptide antagonist astressin both reduced vascular permeability triggered by Ucn but not that by Substance P or histamine. In contrast, the peptide antagonist alpha-helical CRH-(9-41) reduced the effect of all three. The vasodilatory effect of Ucn was largely inhibited by pretreatment with H1 receptor antagonists, suggesting that histamine is the major mediator involved in vitro. Neuropeptide depletion of sensory neurons, treatment with the ganglionic blocker hexamethonium, or in situ skin infiltration with the local anesthetic lidocaine did not affect Ucn-induced vascular permeability, indicating that its in situ effect was not mediated through the peripheral nervous system. These results indicate that Ucn is one of the most potent triggers of rat mast cell degranulation and skin vascular permeability. This effect of Ucn may explain stress-induced disorders, such as atopic dermatitis or psoriasis, and may lead to new forms of treatment.  (+info)

Cytokine-mediated inflammatory hyperalgesia limited by interleukin-4. (6/5252)

1. The effect of IL-4 on responses to intraplantar (i.pl.) carrageenin, bradykinin, TNFalpha, IL-1beta, IL-8 and PGE2 was investigated in a model of mechanical hyperalgesia in rats. Also, the cellular source of the IL-4 was investigated. 2. IL-4, 30 min before the stimulus, inhibited responses to carrageenin, bradykinin, and TNFalpha, but not responses to IL-1beta, IL-8 and PGE2. 3. IL-4, 2 h before the injection of IL-1beta, did not affect the response to IL-1beta, whereas IL-4, 12 or 12+2 h before the IL-1beta, inhibited the hyperalgesia (-30%, -74%, respectively). 4. In murine peritoneal macrophages, murine IL-4 for 2 h before stimulation with LPS, inhibited (-40%) the production of IL-1beta but not PGE2. Murine IL-4 (for 16 h before stimulation with LPS) inhibited LPS-stimulated PGE2 but not IL-1beta. 5. Anti-murine IL-4 antibodies potentiated responses to carrageenin, bradykinin and TNFalpha, but not IL-1beta and IL-8, as well as responses to bradykinin in athymic rats but not in rats depleted of mast cells with compound 40/80. 6. These data suggest that IL-4 released by mast cells limits inflammatory hyperalgesia. During the early phase of the inflammatory response the mode of action of the IL-4 appears to be inhibition of the production TNFalpha, IL-1beta and IL-8. In the later phase of the response, in addition to inhibiting the production of pro-inflammatory cytokines, IL-4 also may inhibit the release of PGs.  (+info)

Tranilast suppresses vascular chymase expression and neointima formation in balloon-injured dog carotid artery. (7/5252)

BACKGROUND: Activation of vascular chymase plays a major role in myointimal hypertrophy after vascular injury by augmenting the production of angiotensin (ANG) II. Because chymase is synthesized mainly in mast cells, we assumed that the chymase-dependent ANG II formation could be downregulated by tranilast, a mast cell-stabilizing antiallergic agent. We have assessed inhibitory effects of tranilast on neointima formation after balloon injury in the carotid artery of dogs, which share a similar ANG II-forming chymase with humans, and further explored the pathophysiological significance of vascular chymase. METHODS AND RESULTS: Either tranilast (50 mg/kg BID) or vehicle was orally administered to beagles for 2 weeks before and 4 weeks after balloon injury. Four weeks after the injury, remarkable neointima was formed in the carotid arteries of vehicle-treated dogs. Chymase mRNA levels and chymaselike activity of vehicle-treated injured arteries were increased 10.2- and 4.8-fold, respectively, those of uninjured arteries. Angiotensin-converting enzyme (ACE) activity was slightly increased in the injured arteries, whereas ACE mRNA levels were not. Tranilast treatment completely prevented the increase in chymaselike activity, reduced the chymase mRNA levels by 43%, and decreased the carotid intima/media ratio by 63%. In vehicle-treated injured arteries, mast cell count in the adventitia showed a great increase, which was completely prevented by the tranilast treatment. Vascular ACE activity and mRNA levels were unaffected by tranilast. CONCLUSIONS: Tranilast suppressed chymase gene expression, which was specifically activated in the injured arteries, and prevented neointima formation. Suppression of the chymase-dependent ANG II-forming pathway may contribute to the beneficial effects of tranilast.  (+info)

Terreic acid, a quinone epoxide inhibitor of Bruton's tyrosine kinase. (8/5252)

Bruton's tyrosine kinase (Btk) plays pivotal roles in mast cell activation as well as in B cell development. Btk mutations lead to severe impairments in proinflammatory cytokine production induced by cross-linking of high-affinity IgE receptor on mast cells. By using an in vitro assay to measure the activity that blocks the interaction between protein kinase C and the pleckstrin homology domain of Btk, terreic acid (TA) was identified and characterized in this study. This quinone epoxide specifically inhibited the enzymatic activity of Btk in mast cells and cell-free assays. TA faithfully recapitulated the phenotypic defects of btk mutant mast cells in high-affinity IgE receptor-stimulated wild-type mast cells without affecting the enzymatic activities and expressions of many other signaling molecules, including those of protein kinase C. Therefore, this study confirmed the important roles of Btk in mast cell functions and showed the usefulness of TA in probing into the functions of Btk in mast cells and other immune cell systems. Another insight obtained from this study is that the screening method used to identify TA is a useful approach to finding more efficacious Btk inhibitors.  (+info)

TY - JOUR. T1 - Involvement of p38 MAP kinase and Smad3 in TGF-β-mediated mast cell functions. AU - Funaba, Masayuki. AU - Ikeda, Teruo. AU - Murakami, Masaru. AU - Ogawa, Kenji. AU - Nishino, Yoshii. AU - Tsuchida, Kunihiro. AU - Sugino, Hiromu. AU - Abe, Matanobu. PY - 2006/12/1. Y1 - 2006/12/1. N2 - Transforming growth factor-β (TGF-β) modulates functions of bone marrow-derived cultured mast cells (BMMCs); cell maturation (up-regulation of mouse mast cell proteases (mmcps)), growth arrest and migration. We investigated the roles of p38 MAP kinase and Smad3 in TGF-β-mediated cell responses in BMMCs. Treating BMMCs with TGF-β induced the phosphorylation of p38 within 2 h and persisted for 24 h. The involvement of p38 in TGF-β-induced cell responses depended upon mast cell functions; it was necessary for up-regulation of mmcp-1 and migration, but not for up-regulation of mmcp-7 and inhibition of metabolic activity. New protein synthesis was required for the up-regulation of mmcp-1 but not ...
Mast cells may activate fibroblasts and contribute to remodeling processes in the lung. However, the mechanism behind these actions needs to be further investigated. Fibroblasts are major regulators of on-going remodeling processes. Protease activated receptor 2 (PAR2) expressed by fibroblasts may be activated by serine proteases, such as the mast cell mediator tryptase. The objective in this study was to investigate the effects of mast cells and specifically mast cell tryptase on fibroblast migration and the role of PAR2 activation. Human lung fibroblasts (HFL-1) were cultured together with human peripheral blood-derived mast cells or LAD2 mast cells and stimulated with either conditioned medium from LAD2 cells or tryptase. Analyses of immunological stimulation of mast cells by IgE/anti IgE in the co-culture system were also performed. The importance of PAR2 activation by mast cells and mast cell tryptase for the migratory effects of fibroblasts was investigated by pre-treatment with the PAR2
TY - JOUR. T1 - Proliferative quiescence of normal mast cells resembles that of cold-sensitive mutant mastocytoma cells. Dominant expression of the quiescent state in heterokaryons. AU - Laeng, H.. AU - Harris, David T.. AU - Schindler, R.. PY - 1985. Y1 - 1985. N2 - Normal murine peritoneal mast cells were fused to serum-deprived, non-proliferating cells of a cultured subline (41-SB-4) of the P-815 murine mastocytoma. Upon reincubation in medium containing 10% horse serum for 48 h, mono- and binuclear 41-SB-4 cells reentered S phase of the cell cycle, while mast cell × 41-SB-4 heterokaryons as well as mono- and binuclear mast cells remained in proliferative quiescence, indicating dominant expression of the quiescent state of mast cells. The quiescent state of normal mast cells thus resembles that of cold-sensitive (cs) mutant cells (21-F) of the undifferentiated P-815 mastocytoma: at the non-permissive temperature of 33 °C, the 21-F cells were found to enter a state of quiescence which is ...
Background: Mast cells infiltrate the bronchial smooth muscle (BSM) in asthmatic patients, but the mechanism of mast cell adhesion is still unknown. The adhesion molecules CD44 (i.e. hyaluronate receptor) and CD51 (i.e. vitronectin receptor) are widely expressed and bind to many extracellular matrix (ECM) proteins. The aims of the study are (i) to identify the role of ECM in mast cell adhesion to BSM and (ii) to examine the role of CD51 and CD44 in this adhesion.. Methods: Human lung mast cells, human mast cell line (HMC-1), and BSM cells from control donors or asthmatic patients were cultured in the presence/absence of various cytokines. Mast cell-BSM interaction was assessed using 3H-thymidine-pulsed mast cells, confocal immunofluorescence, or electron microscopy. Adhesion molecules expression and collagen production on both cell types were evaluated by quantitative RT-PCR, western blot, and flow cytometry.. Results: Mast cell adhesion to BSM cells mostly involved type I collagen of the ECM. ...
BACKGROUND: In asthma and other allergic disorders, the activation of mast cells by IgE and antigen induces the cells to release histamine and other mediators of inflammation, as well as to produce certain cytokines and chemokines. To search for new mast cell products, we used complementary DNA microarrays to analyze gene expression in human umbilical cord blood-derived mast cells stimulated via the high-affinity IgE receptor (Fc(epsilon)RI).. RESULTS: One to two hours after Fc(epsilon)RI-dependent stimulation, more than 2,400 genes (about half of which are of unknown function) exhibited 2-200 fold changes in expression. The transcriptional program included changes in the expression of IL-11 and at least 30 other cytokines and chemokines. Human mast cells secreted 130-529 pg of IL-11/106 cells by 6 h after stimulation with anti-IgE.. CONCLUSION: Our initial analysis of the transcriptional program induced in in vitro-derived human mast cells stimulated via the Fc(epsilon)RI has identified many ...
Mast cell activation causes degranulation and release of cytokines, thereby promoting inflammation. The aim of this study was to investigate the inhibitory effect of CDK4/6 inhibition on mast cell activation in vitro and in vivo. RBL-2H3 rat basophilic leukemia cells (BLCs) and mouse bone marrow-derived mast cells (BMMCs) were sensitized with anti-dinitrophenol (DNP) immunoglobulin (Ig)E antibodies, stimulated with DNP-human serum albumin (HSA) antigens, and treated with the CDK4/6 inhibitor palbociclib. Histological stains were applied to reveal cytomorphological changes. Murine IgE-mediated passive cutaneous anaphylaxis (PCA) and ovalbumin (OVA)-induced active systemic anaphylaxis (ASA) models were used to examine palbociclib effects on allergic reactions in vivo. Western blots were performed to detect the expression of cell signaling molecules associated with mast cell activation. Activated BLCs and BMMCs released copious granule-related mediators (histamine and β-hexosaminidase), which was reduced
TY - JOUR. T1 - Mast cell deficiency results in the accumulation of preadipocytes in adipose tissue in both obese and non-obese mice. AU - Ishijima, Yasushi. AU - Ohmori, Shinya. AU - Ohneda, Kinuko. PY - 2014. Y1 - 2014. N2 - Mast cells have been suggested to play key roles in adipogenesis. We herein show that the expression of preadipocyte, but not adipocyte, marker genes increases in the white adipose tissue of mast cell-deficient (KitW-sh/W-sh) mice under both obese and non-obese conditions. In vitro culturing with adipogenic factors revealed increased adipocytes differentiated from the KitW-sh/W-sh stromal vascular fraction, suggesting the accumulation of preadipocytes. Moreover, the increased expression of preadipocyte genes was restored by mast cell reconstitution in the KitW-sh/W-sh mice. These results suggest positive effects of mast cells on the preadipocyte to adipocyte transition under both physiological and pathological conditions.. AB - Mast cells have been suggested to play key ...
Interactions between products of the mouse W locus, which encodes the c-kit tyrosine kinase receptor, and the Sl locus, which encodes a ligand for c-kit receptor, which we have designated stem cell factor (SCF), have a critical role in the development of mast cells. Mice homozygous for mutations at either locus exhibit several phenotypic abnormalities including a virtual absence of mast cells. Moreover, the c-kit ligand SCF can induce the proliferation and maturation of normal mast cells in vitro or in vivo, and also can result in repair of the mast cell deficiency of Sl/Sld mice in vivo. We now report that administration of SCF intradermally in vivo results in dermal mast cell activation and a mast cell-dependent acute inflammatory response. This effect is c-kit receptor dependent, in that it is not observed when SCF is administered to mice containing dermal mast cells expressing functionally inactive c-kit receptors, is observed with both glycosylated and nonglycosylated forms of SCF, and ...
Intravital multiphoton microscopy has provided insightful information of the dynamic process of immune cells in vivo. However, the use of exogenous labeling agents limits its applications. There is no method to perform functional imaging of mast cells, a population of innate tissue-resident immune cells. Mast cells are widely recognized as the effector cells in allergy. Recently their roles as immunoregulatory cells in certain innate and adaptive immune responses are being actively investigated. Here we report in vivo mouse skin mast cells imaging with two-photon microscopy using endogenous tryptophan as the fluorophore. We studied the following processes. 1) Mast cells degranulation, the first step in the mast cell activation process in which the granules are released into peripheral tissue to trigger downstream reactions. 2) Mast cell reconstitution, a procedure commonly used to study mast cells functioning by comparing the data from wild type mice, mast cell-deficient mice, and mast-cell deficient
c-kit ligand (KL) activated mouse bone marrow-derived mast cells (BMMC) for the dose- and time-dependent release of arachidonic acid from cell membrane phospholipids, with generation of leukotriene (LT) C4 in preference to prostaglandin (PG)D2. KL at concentrations of 10 ng/ml elicited half-maximal eicosanoid generation and at concentrations of , 50 ng/ml elicited a maximal generation of approximately 15 ng LTC4 and 1 ng PGD2 per 10(6) cells, with 20% net beta-hexosaminidase release 10 min after stimulation. Of the other cytokines tested, none, either alone or in combination with KL, elicited or modulated the immediate phase of mediator release by BMMC, indicating strict specificity for KL. Activation of BMMC in response to KL was accompanied by transient phosphorylation of cytosolic phospholipase A2 and reversible translocation of 5-lipoxygenase to a cell membrane fraction 2-5 min after stimulation, when the rate of arachidonic acid release and LTC4 production were maximal. BMMC continuously ...
Resveratrol, a polyphenol abundant in peanuts, red wine and the skin of grapes, has been shown to have anti-cancer, anti-oxidant and anti-inflammatory activities, and may also have beneficial effects on allergic inflammation. We investigated the effects of resveratrol on human mast cell activation in comparison to the anti-allergy drug tranilast. In LAD2 mast cells, both resveratrol and tranilast inhibited degranulation induced by the mast cell activators substance P, IgE/anti-IgE, and compound 48/80. Resveratrol inhibition was immediate, preventing degranulation when added simultaneously to physiological stimuli, and the effect was sustained for up to 24 hrs. The inhibitory effect was not cAMP dependent, but may be attributable to calcium modulation, as resveratrol, and to a lesser extent tranilast, prevented substance P-induced increases in intracellular calcium. Resveratrol attenuated substance P-induced TNF and MCP-1 production and inhibited IgE-mediated release of cysteinyl leukotrienes, whereas
TY - JOUR. T1 - Leflunomide inhibits PDK1/Akt pathway and induces apoptosis of human mast cells. AU - Sawamukai, Norifumi. AU - Saito, Kazuyoshi. AU - Yamaoka, Kunihiro. AU - Nakayamada, Shingo. AU - Ra, Chisei. AU - Tanaka, Yoshiya. PY - 2007/11/15. Y1 - 2007/11/15. N2 - Mast cells release many inflammatory mediators that play an important role not only in allergic diseases but also in chronic inflammatory diseases, autoimmune diseases, and others. A lot of mast cells exist in synovium of rheumatoid arthritis, and it is known that synovitis does not occur in mast cell-deficient mice. Thus, it is thought that mast cells play a very important role in rheumatoid arthritis pathogenesis. Leflunomide is a drug used clinically in the treatment of rheumatoid arthritis. We used clinical doses of 2-cyano-3-hydroxy-N-(4-trifluoromethylphenyl)-butenamide (A77 1726), which is an active metabolite of leflunomide, and decreased the number of viable human primary mast cells in a concentration-dependent manner. ...
Exposure to monomeric IgE in vitro markedly upregulated the ability of mature mouse peritoneal mast cells or mouse BMCMCs or cloned mast cells to bind IgE. Two separate lines of evidence indicate that this response largely, if not entirely, reflected the increased surface expression of FcεRI. First, while mouse mast cells also express FcγRII/ III ((17), (18)), which can bind IgE immune complexes ((18)), virtually all of the binding of monomeric IgE to mouse mast cells that is detectable under the conditions used in our experiments reflects binding of the ligand to a single class of high affinity binding sites, i.e., FcεRI ((18)). Second, we used anti-IgE to immunoprecipitate surface-bound IgE, and associated IgE receptors, from lysates of BMCMCs that had been first incubated with or without IgE at 5 μg/ml for 21 h and then exposed briefly to excess IgE just before recovery for flow cytometry and Western blot analysis. We found that, in comparison to aliquots of the same mast cell population ...
TY - JOUR. T1 - Progress in allergy signal research on mast cells. T2 - Signal regulation of multiple mast cell responses through FcεRI. AU - Yamasaki, Shou. AU - Saito, Takashi. PY - 2008/4/8. Y1 - 2008/4/8. N2 - The crosslinking of FcεRI by IgE and antigen (Ag) on mast cells initiates activation cascades that lead to allergic responses. Although it was thought that IgE binding to FcεRI is a passive sensitization, recent reports suggest that IgE actively promotes mast cell survival in the absence of Ag. However, it is largely unknown how these distinct responses are delivered through the same receptor, FcεRI, depending on the types of stimli. As an underlying molecular mechanism for the generation of diverse responses through FcεRI, we found that the quantity and the duration of the signal through the FcεRI γ chain (FcRγ) determine different mast cell responses. Furthermore, FcRγ-mediated sustained Erk activation is critical for IgE-induced mast cell survival through autocrine ...
In this study the diversity of mast cell proteases and some of the factors regulating mast cell growth and protease expression were examined in rodents. Five proteases were isolated from mouse small intestinal mucosa and their substrate specificities defined. The isolated proteases were all of mast cell origin and were chymotrypsin-like in their substrate specificities. The proteases were all identified as variants of mouse mast cell protease-1 which differed only in their carbohydrate moieties. Despite the fact that these enzymes shared a common core polypeptide they all differed significantly in the rate at which they hydrolysed synthetic substrates and in the rates at which they were inhibited by α1-proteinase inhibitor. A related, but distinct protease was isolated from peritoneal cavity mast cells of mice. This enzyme, also a chymase, had N-terminal sequence identity with mouse mast cell protease-4. This enzyme was not inhibited by α1-proteinase inhibitor. Factors which regulate mast cell ...
Mast cells are best known for their function in hypersensitive reactions, where aggregation of FcRI leads to the release of mast cell mediators leading to hypersensitive symptoms. although activation-induced success is certainly suffered, suggesting a minimal function for Bcl-XL, Bcl-2, Mcl-1 and Bcl-w. Reducing but not really amounts by siRNA inhibited activation-induced mast cell success. We also demonstrate that mast cell phrase of Bfl-1 is certainly raised in birch-pollen-provocated epidermis and in lesions of atopic dermatitis and psoriasis sufferers. Used jointly, our outcomes high light Bfl-1 as a main effector in activation-induced individual mast cell success. Launch Mast cells are known to end up being central regulators and effectors in allergic illnesses. When a multivalent antigen binds to IgE occupying the high affinity receptor for IgE (FcRI), receptor aggregation and following mast cell account activation takes place. This total result in mast cell degranulation, adjustments in ...
The mechanism of chronic mast cell activation in asthma is unclear. Monomeric immunoglobulin (Ig)E in the absence of allergen induces mediator release from rodent mast cells, indicating a possible role for IgE in the continued activation of mast cells within the asthmatic bronchial mucosa. In this study it was investigated whether monomeric IgE induces Ca2+ influx and mediator release from human lung mast cells (HLMC). Purified HLMC were cultured for 4 weeks and then exposed to monomeric human myeloma IgE. Ratiometric Ca2+ imaging was performed on single fura-2-loaded cells. Histamine release was measured by radioenzymatic assay; leukotriene C4 (LTC4) and interleukin (IL)-8 were measured by ELISA. At concentrations experienced in vivo, monomeric IgE induced dose-dependent histamine release, LTC4 production and IL-8 synthesis. This was associated with a rise in cytosolic free Ca2+. Enhanced histamine release was still evident 1 week after initial exposure to IgE suggesting that continued exposure ...
Previous in vitro studies have shown biphasic effects of adenosine on mast cell activity; however, the receptor subtypes that mediate the inhibitory effects of adenosine are still controversial (Peachell et al., 1991; Yip et al., 2009). Mast cells express two distinct Gs-coupled adenosine receptors; their biologic roles have not been comprehensively defined, especially in vivo. Since activation of Gs-coupled adenosine receptors increases intracellular cAMP, we hypothesized that the inhibitory effects of adenosine on mast cells are mediated by the Gs-coupled adenosine receptors. In this study, we used both genetically modified animal models and mast cell cultures to comprehensively investigate the role of Gs-coupled adenosine receptors on mast cells both in vitro and in vivo. First, our data demonstrate a potent inhibitory effect of the nonhydrolyzable adenosine analog NECA on IgE-induced mast cell degranulation; this inhibitory effect of NECA was abolished by the genetic deletion of the A2B but ...
TY - JOUR. T1 - Time- and concentration-dependent effects of exogenous serotonin and inflammatory cytokines on mast cell function. AU - Gruba, Sarah M.. AU - Meyer, Audrey F.. AU - Manning, Benjamin M.. AU - Wang, Yiwen. AU - Thompson, John W.. AU - Dalluge, Joseph J.. AU - Haynes, Christy L.. PY - 2014/2/21. Y1 - 2014/2/21. N2 - Mast cells play a significant role in both the innate and adaptive immune response; however, the tissue-bound nature of mast cells presents an experimental roadblock to performing physiologically relevant mast cell experiments. In this work, a heterogeneous cell culture containing primary culture murine peritoneal mast cells (MPMCs) was studied to characterize the time-dependence of mast cell response to allergen stimulation and the time- and concentration-dependence of the ability of the heterogeneous MPMC culture to uptake and degranulate exogenous serotonin using high performance liquid chromatography (HPLC) coupled to an electrochemical detector. Additionally, ...
To our knowledge, this study is the first to report a regulatory function of tetraspanin CD151 in mast cells. Moreover, it is one of the first reports, to our knowledge, addressing the signaling mechanism of modulation of mast cell activation by any member of the tetraspanin family. In the present study, we demonstrated that CD151 deficiency exacerbated late-phase allergic inflammation in mice in vivo and enhanced proinflammatory cytokine production by cultured BMMCs ex vivo. Moreover, BMMCs deficient in CD151 showed enhanced and sustained FcεRI-induced ERK1/2 and Akt phosphorylation compared with WT cells. Conversely, CD151 deficiency had no effect on mast cell degranulation or the acute phase of PCA. Thus, our data demonstrate that the tetraspanin CD151 functions to selectively inhibit late-phase anaphylaxis responses and the de novo synthesis of cytokines by activated mast cells.. Mast cells possess mechanisms for fine tuning cellular activation that allow initial FcεRI-mediated signaling ...
Latexin, a protein possessing inhibitory activity against rat carboxypeptidase A1 (CPA1) and CPA2, is expressed in a neuronal subset in the cerebral cortex and cells in other neural and non-neural tissues of rat. Although latexin also inhibits mast-cell CPA (MCCPA), the expression of latexin in rat mast cells has not previously been confirmed. In the present study we examined the expression and subcellular localization of latexin in rat peritoneal mast cells. Western blot and reverse-transcriptase-mediated PCR analyses showed that latexin was contained and expressed in the rat peritoneal mast cells. Immunocytochemically, latexin immunofluorescence was localized on granular structures distinct from MCCPA-, histamine- or cathepsin D-immunopositive granules. Immunoelectron microscopy revealed that latexin was associated with a minority population of granules. The latexin-associated granules were separated from MCCPA- or histamine-containing granules on a self-generating density gradient of ...
Background and objective:Gingival bleeding reduction in smokers has been associated with decreased blood vessel density. The mechanism of suppressive effect of cigarette smoking on blood vessel density is not precisely defined. The aim of this study was to evaluate the impact of smoking on angiogenesis by assessing mast cells density and VEGF expression in chronic periodontitis. Materials& Methods: 52 paraffin embedded block of gingiva tissues with periodontitis obtained from 30 nonsmokers and 22 smokers undergoing flap surgery were examined immunohistochemically for VEGF expression. Mast cell counts was completed on toluidine blue stained slides. Exposure to cigarette smoking was calculated by the number of packs × year. Patients were classified into 4 groups based on the number of smoked cigarettes. The correlation between VEGF expression and mast cell counts was evaluated and compared in nonsmokers and smokers. Results: The mean number of mast cells (p=0.004) and average value of VEGF expression (p
Author(s): Hata, D; Kawakami, Y; Inagaki, N; Lantz, CS; Kitamura, T; Khan, WN; Maeda-Yamamoto, M; Miura, T; Han, W; Hartman, SE; Yao, L; Nagai, H; Goldfeld, AE; Alt, FW; Galli, SJ; Witte, ON; Kawakami, T | Abstract: We investigated the role of Brutons tyrosine kinase (Btk) in FcepsilonRI-dependent activation of mouse mast cells, using xid and btk null mutant mice. Unlike B cell development, mast cell development is apparently normal in these btk mutant mice. However, mast cells derived from these mice exhibited significant abnormalities in FcepsilonRI-dependent function. xid mice primed with anti-dinitrophenyl monoclonal IgE antibody exhibited mildly diminished early-phase and severely blunted late-phase anaphylactic reactions in response to antigen challenge in vivo. Consistent with this finding, cultured mast cells derived from the bone marrow cells of xid or btk null mice exhibited mild impairments in degranulation, and more profound defects in the production of several cytokines, upon FcepsilonRI
phdthesis{d683936c-1726-4ede-86a7-193f0162cd84, abstract = {Mast cell are found throughout the body, but are especially prominent in tissues that have direct contact with the external milieu such as the skin, gastrointestinal tract and lungs. Mast cells are commonly recognized for their detrimental role in allergic reactions and can, upon activation through the high-affinity receptor for IgE (FcεRI), rapidly produce and secrete many of the mediators responsible for the typical symptoms in urticaria, asthma and rhinitis. However, increasing amount of data show that mast cells have important, even vital, roles in host defence against bacteria, viruses, parasites and venoms. Mast cells exist as two different subtypes, MCT (mucosal mast cells) and MCTC (connective tissue mast cells). These two subtypes differ in their molecular expression and distribution in the body. MCT are for example the dominating subtype in the lungs, while MCTC are most common in the skin and the gastrointestinal tract. ...
TY - JOUR. T1 - Diamine oxidase-gold ultrastructural localization of histamine in isolated human lung mast cells stimulated to undergo anaphylactic degranulation and recovery in vitro. AU - Dvorak, Ann M.. AU - Morgan, Ellen S.. AU - Schleimer, Robert P.. AU - Lichtenstein, Lawrence M.. PY - 1996/1/1. Y1 - 1996/1/1. N2 - A new enzyme-affinity-gold ultrastructural method makes use of the affinity of the enzyme, diamine oxidase coupled to gold, for its substrate, histamine, for localization of histamine in isolated human lung mast cells (HLMCs). The method works with routinely prepared ultrastructural samples, thereby allowing precise identification of ultrastructural structures that contain histamine. We used this method to identify the release of histamine from granule stores in anti-immunoglobulin-E (IgE)-stimulated HLMCs and the replacement of histamine in secretory granules of HLMCs during recovery from anaphylactic degranulation in vitro. The findings show that electron-dense granules in ...
Mast cells (MC) have been mainly studied as key effectors in allergic diseases and inflammatory conditions such hypersensitivity reactions, asthma, atopic dermatitis and multiple sclerosis. Following the crosslinkage of membraneous FcεRI, by antigens, a large number of chemical mediators are secreted. This event leads to the recruitment and activation of basophils and eosinophils that sustain the inflammatory response. The role of mast cells, however, is not limited to the initiation of allergic response but they are also fundamental players in the innate immune response; for example they can be activated directly by pathogens through a family of pattern recognition receptors called Toll-like receptors (TLRs). In particular, TLR2 and 4 seem to be crucial to the mast cell response to pathogens. In rodents, mast cells respond to lipopolysaccharide through their TLR4s by the release of pro-inflammatory cytokines without concurrent degranulation or they can degranulate following peptidoglycan ...
TY - JOUR. T1 - Pediatric Expression of Mast Cell Activation Disorders. AU - Broesby-Olsen, Sigurd. AU - Carter, Melody. AU - Kjaer, Henrik Fomsgaard. AU - Mortz, Charlotte Gotthard. AU - Møller, Michael Boe. AU - Kristensen, Thomas Kielsgaard. AU - Bindslev-Jensen, Carsten. AU - Agertoft, Lone. PY - 2018/8. Y1 - 2018/8. N2 - Mast cell activation disorders is a term proposed to cover diseases and conditions related to activation of mast cells and effects of mast cell mediators. In its broadest sense, the term encompasses a wide range of diseases from allergic asthma to rhinoconjunctivitis, urticaria, food allergy, anaphylaxis, mastocytosis, and other conditions where MC activation is contributing to the pathogenesis. This article focuses on clinical presentations, challenges, and controversies in pediatric mastocytosis and gives an overview of current knowledge and areas in need of further research.. AB - Mast cell activation disorders is a term proposed to cover diseases and conditions related ...
Expression of Mast Cell Proteases Correlates with Mast Cell Maturation and Angiogenesis during Tumor Progression. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Mast cells donate to allergy through IgE-dependent activation the high-affinity IgE receptor FcεRI. receptor gain-of-function human mast cell line HMC-1. Unlike MS4A2 MS4A2trunc did not traffic to the cytoplasmic membrane but instead was associated with the nuclear membrane. Overexpression of MS4A2trunc induced human lung mast cell death and profoundly inhibited HMC-1 cell proliferation by inducing G2-phase cell cycle arrest and apoptosis. Thus we have identified a novel splice variant of MS4A2 that might be important in the regulation of human mast cell proliferation and survival. This finding demonstrates that the MS4A2 gene has multiple roles extending beyond the rules of acute sensitive reactions. By understanding the systems regulating its function it could be feasible to induce its manifestation in mast cells cells had been then transformed using the MS4A2 clones and plated from agar plates including 100 μg/ml ampicillin with 100 μl of IPTG and 20 μl of X-galactose added. Transformed ...
Mast cells are present in limited numbers in normal human synovium, but in rheumatoid arthritis and other inflammatory joint diseases this population can expand to constitute 5% or more of all synovial cells. Recent investigations in a murine model have demonstrated that mast cells can have a critical role in the generation of inflammation within the joint. This finding highlights the results of more than 20 years of research indicating that mast cells are frequent participants in non-allergic immune responses as well as in allergy. Equipped with a diversity of surface receptors and effector capabilities, mast cells are sentinels of the immune system, detecting and delivering a first response to invading bacteria and other insults. Accumulating within inflamed tissues, mast cells produce cytokines and other mediators that may contribute vitally to ongoing inflammation. Here we review some of the non-allergic functions of mast cells and focus on the potential role of these cells in murine and human
Rheumatoid arthritis (RA) is a multifactorial autoimmune disease, which is characterized by inflammation of synovial joints leading to the destruction of cartilage and bone. Infiltrating mast cells can be found within the inflamed synovial tissue, however their role in disease pathogenesis is unclear. Therefore we have studied the role of mast cells during different phases of experimental arthritis. We induced collagen-induced arthritis (CIA), the most frequently used animal model of arthritis, in an inducible mast cell knock-out mouse and determined the effect of mast cell depletion on the development and severity of arthritis. Depletion of mast cells in established arthritis did not affect clinical outcome. However, depletion of mast cells during the preclinical phase resulted in a significant reduction in arthritis. This reduction coincided with a decrease in circulating CD4+ T cells and inflammatory monocytes but not in the collagen-specific antibody levels. Mast cell depletion resulted in reduced
In addition to their central role in allergy, mast cells are involved in a wide variety of cellular interactions during homeostasis and disease. In this review, we discuss the ability of mast cells to extend their mechanisms for intercellular communication beyond the release of soluble mediators. These include formation of mast cell synapses on antigen presenting surfaces, as well as cell-cell contacts with dendritic cells and T cells. Release of membrane bound exosomes also provide for the transfer of antigen, mast cell proteins, and RNA to other leukocytes. With the recognition of the extended role mast cells have during immune modulation, further investigation of the processes in which mast cells are involved is necessary. This reopens mast cell research to exciting possibilities, demonstrating it to be an immunological frontier ...
TY - JOUR. T1 - BLT2 is upregulated in allergen-stimulated mast cells and mediates the synthesis of Th2 cytokines. AU - Cho, Kyung Jin. AU - Seo, Ji Min. AU - Lee, Min-Goo. AU - Kim, Jae-Hong. PY - 2010/11/15. Y1 - 2010/11/15. N2 - Mast cells are effector cells that mediate the allergic response through Ag stimulation of IgE bound to FcεRI. In allergic reactions, cross-linking of the surface receptors for IgE on mast cells results in the synthesis of Th2 cytokines such as IL-4 and IL-13, which are critical for the initiation and progression of the allergic response. Despite the important roles of these cytokines, the signaling mechanism by which Ag stimulation mediates the production of IL-4 and IL-13 in mast cells is not clearly understood. In the present study, we found that Ag-stimulated bone marrow-derived mast cells (BMMCs) highly upregulated the expression of BLT2, a leukotriene B 4 receptor, and that blockade of BLT2 with the specific antagonist LY255283 or small interfering RNA ...
Mast cells are connected with swelling and fibrosis. led to renin-dependent protracted circulation recovery. This demonstrates that mast cell renin is PIK-294 supplier definitely energetic in situ and the following ANG II can modulate intrarenal vascular level of resistance in the UUO kidney. Jointly, the data Rabbit Polyclonal to STEA3 demonstrate that mast cells are essential to the advancement of renal fibrosis in the 14-day time UUO kidney. Since renin is definitely present in human being kidney mast cells, our function recognizes potential focuses on in the treatment of renal fibrosis. is definitely the quantity of photo slides for a provided pet. Renin activity (ANG I radioimmunoassay). Renin activity was assessed in separated mast cell lysate (rat kidney and human being kidney), as previously reported (32, 48, 54). The recognition limit was 0.01 pmol (32). Remote mast cells had been lysed in 1 ml of PBS by four cycles of freeze-thaw. The renin-containing lysates had been after that ...
Mast cell (MC) differentiation, survival, and activation are controlled by the membrane tyrosine kinase c-Kit upon interaction with stem cell factor (SCF). Here we describe a single point mutation induced by N-ethyl-N-nitrosurea (ENU) mutagenesis in C57BL/6J mice-an A to T transversion at position 2388 (exon 17) of the c-Kit gene, resulting in the isoleucine 787 substitution by phenylalanine (787F), and analyze the consequences of this mutation for ligand binding, signaling, and MC development. The Kit(787F/787F) mice carrying the single amino acid exchange of c-Kit lacks both mucosal and connective tissue-type MCs. In bone marrow-derived mast cells (BMMCs), the 787F mutation does not affect SCF binding and c-Kit receptor shedding, but strongly impairs SCF-induced cytokine production, degranulation enhancement, and apoptosis rescue. Interestingly, c-Kit downstream signaling in 787F BMMCs is normally initiated (Erk1/2 and p38 activation as well as c-Kit autophosphorylation) but fails to be ...
Mast cells are tissue-resident hematopoietic cells. Because infectious agents enter the host through environmentally exposed barriers, such as the skin, gastrointestinal tract, and respiratory tract, mast cells are poised to be one of the first cell types to respond to invading pathogens. Furthermore, mast cells express a wide array of pattern recognition receptors that endow them with the ability to respond to a broad range of stimuli, such as infections and pathogenic conditions (51). It is well established that mast cells play crucial immune surveillance roles during bacterial and parasitic infections (8, 12). In contrast, the role of mast cells in the immune surveillance of viral infections has received less attention. In the current study, we examined the role of mast cells in sensing IAV infection and initiating the subsequent inflammatory response.. A primary rationale for our work stems from the recent work by Teijaro et al. (6), who demonstrated that blunting the cytokine storm ...
TY - JOUR. T1 - Novel Site-Specific Mast Cell Subpopulations in the Human Lung.. AU - Andersson, Cecilia K. AU - Mori, Michiko. AU - Bjermer, Leif. AU - Löfdahl, Claes-Göran. AU - Erjefält, Jonas. PY - 2009. Y1 - 2009. N2 - BACKGROUND: Lung mast cells are stereotypically divided into connective tissue (MCTC) and mucosal (MCT) mast cells. This study tests the hypothesis that each of these subtypes can be divided further into site-specific populations created by the microenvironment within each anatomic lung compartment. METHODS: To study mast cells under non-inflamed conditions surgical resections and bronchial and transbronchial biopsies from non-smoking individuals were obtained to investigate morphometric and molecular characteristics of mast cell populations in multiple lung structures by immunohistochemistry and electron microscopy. RESULTS: MCT and MCTC coexisted at all compartments with MCT being the prevailing type in bronchi, bronchioles and the alveolar parenchyma. MCTC were more ...
T cell mediated immune responses in the gut can produce enteropathy and malabsorption. We have investigated the relevance of mucosal mast cells (MMC) to the mechanisms of this enteropathy by using graft-versus-host reaction (GvHR) in the rat as a model of mucosal delayed type hypersensitivity. Measurements of mucosal architecture, intraepithelial lymphocytes (IEL) and MMC counts were performed in control and experimental rats, and release of rat mast cell protease II (RMCPII) into the bloodstream was used as an index of MMC activation. In unirradiated rats, jejunal MMC count was increased on day 14 of the GvHR (mean 272/mm2 v 182 in controls, p less than 0.01), as was serum RMCPII (p less than 0.01). Irradiated rats (4.5 Gy, reconstituted with isogeneic spleen cells) had low counts of IEL and crypt hyperplasia seven to 14 days after irradiation. Irradiated rats with GvHR (induced by ip injection of parental strain spleen cells) and studied on days 7, 10 and 14, had significant enteropathy with ...
Mast cells are found in tissues throughout the body where they play important roles in the regulation of inflammatory responses. One characteristic feature of mast cells is their longevity. Although it is well established that mast cell survival is dependent on stem cell factor (SCF), it has not been described how this process is regulated. Herein, we report that SCF promotes mast cell survival through inactivation of the Forkhead transcription factor FOXO3a (forkhead box, class O3A) and down-regulation and phosphorylation of its target Bim (Bcl-2 [B-cell lymphoma-2] interacting modulator of cell death), a Bcl-2 homology 3 (BH3)-only proapoptotic protein. SCF induced a rapid and transient phosphorylation of Akt (protein kinase B) and FOXO3a. SCF treatment prevented up-regulation of Bim protein expression and led to increased Bim phosphorylation. Bim phosphorylation was inhibited by PD98059 and LY294002 treatment, suggesting the involvement of mitogen-activated protein kinase ...
METHODS AND RESULTS Specimens of normal and atherosclerotic human coronary intima from 32 autopsy cases with ages ranging from 13 to 67 years were stained with monoclonal antibodies against the two major proteases of mast cells, tryptase and chymase. Of the tryptase-containing mast cells, a variable proportion (average, 40%; range, 0% to 100%) also contained chymase. In the normal coronary intimas, mast cells amounted to 0.1% of all nucleated cells. In the fatty streaks, this proportion was higher by 9-fold, and in the cap, core, and shoulder regions of atheromas by 5-, 5-, and 10-fold, respectively. Electron and light microscopic studies of mast cells in the shoulder region of atheromas revealed degranulation of mast cells, a sign of their activation, and moreover, that the proportion of activated mast cells was much higher (85%) in this region than in the normal intima (18%). ...
Mast cells are abundantly expressed in synovial tissues and have been proposed to exert proinflammatory effects primarily based on antibody transfer-induced disease models (28, 29). The mode of mast cell activation and the mechanism by which activated-mast cells mediate antigen-induced arthritis are largely unknown and likely complex. We now provide direct in vivo evidence that IL-33 plays a major role in mast cell activation in the context of antigen-induced arthritis. Thus, IL-33 enhanced CIA when ST2−/− mice were reconstituted with BMMCs from WT but not from ST2−/− mice (Fig. 5). Our data also provide mechanisms by which mast cells could promote inflammatory synovitis (Fig. 6). IL-33 induces mast cell production of IL-1, IL-6, IL-13, and a range of chemokines (13, 14, 30) (Fig. 4). Because both IL-1 and IL-6 play crucial roles in the induction of Th17 cells (31-33), a key pathogenic cell type in arthritis (21, 22), our studies indicate a relationship between ST2/IL-33 function and ...
The mast cell possesses within itself granules of especially inflammatory biochemicals meant for use against invading parasites. (Think of these as small bombs that can be released). The mast cell has binding sites on its surface for a special type of antibody called IgE. IgE is produced in response to exposure to antigens typical of parasites (i.e., worm skin proteins, or similarly shaped proteins). IgE antibodies, which are shaped like tiny Ys, find their way to a tissue mast cell and perch there. With enough exposure to the antigen in question, the mast cell may be covered with Y- shaped IgE antibodies like the fluff of a dandelion. The mast cell is said, at this point, to be sensitized ...
The mast cell possesses within itself granules of especially inflammatory biochemicals meant for use against invading parasites. (Think of these as small bombs that can be released). The mast cell has binding sites on its surface for a special type of antibody called IgE. IgE is produced in response to exposure to antigens typical of parasites (i.e., worm skin proteins, or similarly shaped proteins). IgE antibodies, which are shaped like tiny Ys, find their way to a tissue mast cell and perch there. With enough exposure to the antigen in question, the mast cell may be covered with Y- shaped IgE antibodies like the fluff of a dandelion. The mast cell is said, at this point, to be sensitized ...
TY - JOUR. T1 - ATP-induced pore formation in the plasma membrane of rat peritoneal mast cells. AU - Hofmann, Polly. AU - Metzger, Joseph M.. AU - Greaser, Marion L.. AU - Moss, Richard L.. PY - 1990/3/1. Y1 - 1990/3/1. N2 - Various functional roles for myosin light chain 2 (LC2) have been suggested on the basis of numerous and predominantly in vitro biochemical studies. Using skinned fibers from rabbit psoas muscle, the present study examines the influence of partial removal of LC2 on isometric tension, stiffness, and maximum velocity of shortening at various levels of activation by Ca2+. Isometric tension, stiffness, and velocity of shortening were measured at pCa values between 6.6 and 4.5 (a) in a control fiber segment, (b) in the same fiber segment after partial removal of LC2, and (c) after recombination with LC2. The extraction solution contained 20 mM EDTA, 20 or 50 mM KC1, and either imidazole or PO4 2− as a pH buffer (pH 7.0). The amount of LC2 extracted varied with the temperature, ...
The nonselective beta-adrenoceptor agonist, isoprenaline (pD2; 8.8 +/- 0.2), and selective beta2-adrenoceptor agonists, clenbuterol (9.2 +/- 0.4) and salbutamol (7.1 +/- 0.1), inhibited the immunoglobulin E-mediated release of histamine from human lung mast cells in a concentration-dependent manner. …
Mast cells play a central role in the pathogenesis of allergic reaction. Activation of mast cells by antigens is strictly dependent on the influx of extracellular calcium that involves a complex interaction between signalling molecules located within the cells. We have previously reported that tHGA, an active compound originally isolated from a local shrub known as Melicope ptelefolia, prevented IgE-mediated mast cell activation and passive systemic anaphylaxis by suppressing the release of interleukin-4 (IL-4) and tumour necrosis factor (TNF)-α from activated rat basophilic leukaemia (RBL)-2H3 cells. However, the mechanism of action (MOA) as well as the molecular target underlying the mast cell stabilising effect of tHGA has not been previously investigated. In this study, DNP-IgE-sensitised RBL-2H3 cells were pre-treated with tHGA before challenged with DNP-BSA. To dissect the MOA of tHGA in IgE-mediated mast cell activation, the effect of tHGA on the transcription of IL-4 and TNF-α mRNA was ...
With regard to mast cells, Im always looking at mesenchymal and histiocytic tumors of human skin and in my experience dendritic connective tissue mast cells also stain for CD68 (KP-1) a lysosomal glycoprotein. In fact, Im having to do toluidine blue (which gets most of the stromal mast cells,no?) to distinguish these from the histiocytes (some CD68+) and dermal dendritic cells (CD68 negative) in a series of histiocytomas. Havent noticed many mucosal (epithelial?) mast cells in skin though. I like to use end mounted individual controls. For our commonly performed stains, (like alcian blue on Gastroesophageal junction and esophagus, iron, PAS, mucin. etc, I choose a block from that days work, (eg a small colon polyp for alcian blue/muci) and pickup some extra sections. That way, I can pick up a section on the end of each special slide,each has its own control. I also keep common control blocks handy and do this with ER/PR/Her 2 neu, iron, retic, fungus etc. Mine is a one person lab so the ...
The á2â1 integrin is expressed on many cell types throughout the immune system. Expression of the á2â1 integrin on mast cells is required for the early innate immune response to Listeria monocytogenes. Interaction between the á2â1 integrin and Listeria occurs through C1q within a Listeria immune complex, but is not sufficient for activation suggesting an additional co-receptor is required for activation. We demonstrate that Listeria immune complex activation of mast cells occurs through crosstalk between the á2â1 integrin and c-met. The best described mechanism of mast cell activation is IgE-mediated degranulation. We examined the mechanism of mediator release by mast cells following activation by Listeria immune complex. Activation by Listeria immune complex results in á2â1 integrin-dependent release of IL-6 from a granule pool that is distinct from known mast cell granules, identifying a novel population of mast cell granules. The á2â1 integrin-dependent early innate immune ...
Mast cells are powerful immune modulators of the tissue microenvironment. Within seconds of activation, these cells release a variety of preformed biologically active products, followed by a wave of mediator synthesis and secretion. Increasing evidence suggests that an intricate network of inhibitory and activating receptors, specific signaling pathways, and adaptor proteins governs mast cell responsiveness to stimuli. Here, we discuss the biological and clinical relevance of negative and positive signaling modalities that control mast cell activation, with an emphasis on novel Fc epsilon RI regulators, immunoglobulin E (IgE)-independent pathways [e.g., Mas-related G protein-coupled receptor X2 (MRGPRX2)], tetraspanins, and the CD300 family of inhibitory and activating receptors.. ...
TY - JOUR. T1 - Mechanism of bradykinin-induced histamine release from rat peritoneal mast cells. AU - Zhao, Qiu E.. AU - Mihara, Takuma. AU - Sugimoto, Yukio. AU - Kamei, Chiaki. PY - 1996/2. Y1 - 1996/2. N2 - Bradykinin at concentrations higher than 2 μM caused a significant histamine release from rat peritoneal mast cells when extracellular Ca2+ was removed from the medium. Under the same experimental conditions, bradykinin increased Ca2+ release from the intracellular Ca store of the rat peritoneal mast cells, and a clear relationship was observed between the magnitude of histamine release and an increase in fluorescence intensity. Addition of Ca2+ to the medium resulted in an inhibition of the response to bradykinin in a concentration-dependent manner. Almost the same results were obtained when Mg2+, Ba2+ and La3+ were added to the medium. Neither B1 nor B2 antagonists caused significant antagonistic effects on histamine release induced by bradykinin. However, B2 antagonists caused a ...
Immune cells like NK cells, T cells, neutrophils and mast cells store high amounts of granule serine proteases, graspases. Graspases are encoded from the mast cell chymase locus. The human locus holds four genes: α-chymase, cathepsin G, and granzymes H and B. In contrast, the mouse locus contains at least 14 genes. Many of these belong to subfamilies not found in human, e.g. the Mcpt8-family. These differences hamper functional comparisons of graspases and of immune cells in the two species. Studies of the mast cell chymase locus are therefore important to better understand the mammalian immune system. In this thesis, the evolution of the mast cell chymase locus was analysed by mapping the locus in all available mammalian genome sequences. It was revealed that one single ancestral gene founded this locus probably over 215 million years ago. This ancestor was duplicated more than 185 million years ago. One copy evolved into the α-chymases, whereas the second copy founded the families of ...
Mast cells are responsible for the majority of allergic conditions. It was originally thought that almost all allergic events were mediated directly only via the high-affinity immunoglobulin E receptors. However, recent evidence showed that many other receptors, such as G protein-coupled receptors and ligand-gated ion channels, are also directly involved in mast cell degranulation, the release of inflammatory mediators such as histamine, serine proteases, leukotrienes, heparin, and serotonin. These mediators are responsible for the symptoms in allergic conditions such as allergic asthma. In recent years, it has been realized that purinergic signaling, induced via the activation of G protein-coupled adenosine receptors and P2Y nucleotide receptors, as well as by ATP-gated P2X receptors, plays a significant role in mast cell degranulation. Both adenosine and ATP can induce degranulation and bronchoconstriction on their own and synergistically with allergens. All three classes of receptors, adenosine, P2X
TY - JOUR. T1 - Role of mast cells and their mediators in failing myocardium under mechanical ventricular support. AU - Akgul, Ahmet. AU - Skrabal, Christian A.. AU - Thompson, Larry O.. AU - Loebe, Matthias. AU - Lafuente, Javier A.. AU - Noon, George P.. AU - Youker, Keith A.. PY - 2004/6/1. Y1 - 2004/6/1. N2 - Background Mast cells have been implicated in tissue remodeling and fibroblast stimulation. We explored the effect of mechanical support by left ventricular assist device (LVAD) in failing myocardium and looked into grade and distribution of interstitial fibrosis, mast cell density, mast cell phenotypes and basic fibroblast growth factor (bFGF) expression pre- and post-LVAD. Methods Myocardial tissue was obtained from 20 patients with end-stage cardiomyopathy at the time of LVAD implantation and LVAD removal and from 7 donor hearts not used for transplantation. Tissue sections were stained for mast cells using tryptase as a marker and the myocardial fibrosis was measured. Double ...
Experimental autoimmune encephalomyelitis (EAE) is a mouse model that reproduces cardinal signs of clinical, histopathological, and immunological features found in Multiple Sclerosis (MS). Mast cells are suggested to be involved in the main inflammatory phases occurring during EAE development, possibly by secreting several autacoids and proteases. Among the latter, the chymase mouse mast cell protease 4 (mMCP-4) can contribute to the inflammatory response by producing endothelin-1 (ET-1). The aim of this study was to determine the impact of mMCP-4 on acute inflammatory stages in EAE. C57BL/6 wild type (WT) or mMCP-4 knockout (KO) mice were immunized with MOG(35-55) plus complete Freunds adjuvant followed by pertussis toxin. Immunized WT mice presented an initial acute phase characterized by progressive increases in clinical score, which were significantly reduced in mMCP-4 KO mice. In addition, higher levels of spinal myelin were found in mMCP-4 KO as compared with WT mice. Finally, whereas EAE ...
Concern about the use of nanomaterials has increased significantly in recent years due to potentially hazardous impacts on human health. Mast cells are critical for innate and adaptive immune responses, often modulating allergic and pathogenic conditions. Mast cells are well known to act in response to danger signals through a variety of receptors and pathways including IL-33 and the IL-1-like receptor ST2. Here, the involvement of mast cells and the IL-33/ST2 axis in pulmonary and cardiovascular responses to multi-walled carbon nanotube (MWCNT) exposure are examined. Toxicological effects of MWCNTs are observed only in mice with a sufficient population of mast cells and are not observed when mast cells are absent or incapable of responding to IL-33. Our findings establish for the first time that mast cells and the IL-33/ST2 axis orchestrates adverse pulmonary and cardiovascular responses to an engineered nanomaterial, giving insight into a previously unknown mechanism of toxicity. This novel mechanism
Primary graft dysfunction (PGD), as characterized by pulmonary infiltrates and high oxygen requirements shortly after reperfusion, is the major cause of early morbidity and mortality after lung transplantation. Donor, recipient and allograft-handling factors are thought to contribute, although new insights regarding pathogenesis are needed to guide approaches to prevention and therapy. Mast cells have been implicated in ischemic tissue injury in other model systems and in allograft rejection, leading to the hypothesis that mast cell degranulation contributes to lung injury following reperfusion injury. We tested this hypothesis in a mouse model of PGD involving reversible disruption of blood flow to one lung. Metrics of injury included albumin permeability, plasma extravasation, lung histopathology, and mast cell degranulation. Responses were assessed in wild-type (Kit +/+ ) and mast cell-deficient (Kit W-sh/W-sh ) mice. Because mouse
TY - JOUR. T1 - Effective mast cell degranulating peptide inhibitors of the IgE/FcεRI receptor interaction. AU - Buku, Angeliki. AU - Keselman, Inna. AU - Lupyan, Dmitry. AU - Mezei, Mihaly. AU - Price, Joseph. PY - 2008/8/1. Y1 - 2008/8/1. N2 - Previous studies with mast cell degranulating (MCD) peptide have shown that peptide [Ala12]MCD 8 was an inhibitor of IgE binding to mast cell receptors. In an attempt to produce increased inhibition, analogs were synthesized that maintained the alanine residue in position 12 in the MCD peptide sequence and were further modified at both termini. Analogs modified at the C-terminus were [Ala12,desLys21]MCD 2 and [Ala 12,d-Lys21]MCD 4. N-terminus modifications were [desLys6-Arg7-His8,Ala12]MCD 1, [Ala6, Ala12]MCD 6, and [Val6,Ala 12]MCD 7. To assess the role of the Proline12, analogs [d-Ala12]MCD 3 and [Meleu12]MCD 5 were also synthesized. The analogs were tested for binding to the IgE receptor in cultured mast cells. Inhibitory activity of IgE-caused ...
Muscle layer alterations such as myositis and fibrosis that could contribute to the pathogenesis of Chagas disease megas are often found both in the esophagus1 3 16 and in the colon2 16. According to Tafuri and Raso16, fibrosis in Chagas megaesophagus sometimes is focal, possibly representing a sequel of myositis and thought to be associated with mast cell infiltrate, and sometimes is diffuse and interstitial without showing a topographic relationship with the inflammation. Andrade & Andrade8, analyzing myocardial fibrosis, also accepted that focal fibrosis could result from scarring of inflammatory foci related to the presence of mast cells. Nonetheless, Andrade & Andrade8 stressed that the pathogenesis of diffuse interstitial fibrosis had not been clarified.. Quantitative studies made on cardiopathic Chagas disease patients7 and on the circular esophagus musculature of chronic Chagas patients without megaesophagus11, have reported a marked increase in the mast cell count in these organs. ...
TY - JOUR. T1 - Ultrastructural immunolocalization of basic fibroblast growth factor in mast cell secretory granules. T2 - Morphological evidence for bFGF release through degranulation. AU - Qu, Zhenhong. AU - Kayton, Robert J.. AU - Ahmadi, Proochista. AU - Liebler, Janice M.. AU - Powers, Michael R.. AU - Planck, Stephen R.. AU - Rosenbaum, James T.. PY - 1998/10. Y1 - 1998/10. N2 - We previously reported that mast cells (MCs) serve as a source of basic fibroblast growth factor (bFGF), a potent angiogenic and mitogenic polypeptide, suggesting that bFGF may mediate MC-related neovascularization and fibroproliferation. Unlike many other growth factors, bFGF lacks a classic peptide sequence for its secretion, and the mechanism(s) for its release remains controversial. Because MCs release a wide spectrum of bioactive products via degranulation, we hypothesized that MC degranulation may be a mechanism of bFGF release and used ultrastructural immunohistochemistry to test the hypothesis. We reasoned ...
It has been suggested that histamine plays an important role in the pathogenesis of cluster headache. In addition, both neurogenic and vascular components have been described during cluster headache attacks without an obvious anatomical link between them. Our ultrastructural observations of human temporal arteries from cluster headache patients and their comparison to those from a control group strongly suggest that mast cells may be this link. Mast cells in both groups show a very close apposition with nerve fibres, suggesting a functional interaction between them. Moreover, in the cluster headache group exclusively, adventitial mast cells show profound morphological modifications suggesting progressive degranulation. These data strongly suggest that mast cells could be directly or indirectly involved in the pathophysiology of cluster headaches. ...
Converging sources of evidence point to a role for inflammation in the development of depression, fatigue and cognitive dysfunction. More precisely, the tryptophan (TRP) catabolism is thought to play a major role in inflammation-induced depression. Mastocytosis is a rare disease in which chronic symptoms, including depression, are related to mast cell accumulation and activation. Our objectives were to study the correlations between neuropsychiatric features and the TRP catabolism pathway in mastocytosis in order to demonstrate mast cells potential involvement in inflammation-induced depression. Fifty-four patients with mastocytosis and a mean age of 50.1 years were enrolled in the study and compared healthy age-matched controls. Depression and stress were evaluated with the Beck Depression Inventory revised and the Perceived Stress Scale. All patients had measurements of TRP, serotonin (5-HT), kynurenine (KYN), indoleamine 2,3-dioxygenase 1 (IDO1) activity (ratio KYN/TRP), kynurenic acid (KA) ...
35. Why are there different sets of criteria for mast cell activation syndrome? What are the differences between them?. To answer this fully, we need to first discuss the history behind some terms.. Mast cell activation syndrome was first used to describe episodes of mast cell mediator release symptoms in a paper published in 2007 (Akin 2007). Specifically, the term was used to detail the experience of patients who had symptoms we commonly associated with mast cell activation, like flushing, hives, and low blood pressure.. However, the patients in this study were all found to have some features of systemic mastocytosis. While they had some of the criteria for an SM diagnosis, they didnt meet all the criteria. These patients sort of looked like SM and quacked like SM but would not cleanly meet the diagnostic criteria. So the author of that paper made a separate diagnostic category for them. He called it monoclonal mast cell activation syndrome.. The use of the word monoclonal is VERY important ...
TY - JOUR. T1 - Heterogeneity of human basophils and mast cells in response to muscle relaxants. AU - Stellato, C.. AU - de Paulis, A.. AU - de Crescenzo, G.. AU - Tatangelo, F.. AU - Rickler, O.. AU - Marone, G.. PY - 1992/6. Y1 - 1992/6. N2 - We investigated the in vitro effects of increasing concentrations (10-5-10-3M) of four muscle relaxants (succinylcholine, d-tubocurarine, vecuronium and atracurium) on histamine release (HR) from human peripheral blood basophils and mast cells isolated from lung parenchyma (HLMC) and skin tissues (HSMC). Basophils released less than 5% of their histamine content when incubated with any one of the muscle relaxants. In contrast, mast cells showed a marked heterogeneity in their response. Succinylcholine did not induce HR from any type of mast cell, and only high concentrations of d-tubocurarine (10-3M) caused HR from HSMC and HLMC. Vecuronium concentration-dependently induced HR from HLMC and HSMC. Atracurium concentration-dependently caused marked HR from ...
Mastocytosis: A disease of Mast Cells. What are Mast Cells ?. Mast cells belong to the family of ´white blood cells´ most of which are produced in our bone marrow. The mast cell was first discovered and described by Paul Ehrlich in 1876. In contrast to most other bone marrow-derived cells, mast cells are not found in the peripheral blood, but are located in the tissues where they reside for many months or even years. Like most white blood cells, mast cells belong to the immune system that helps in the body´s defence against bacteria and other microbes. As part of an alarm system, mast cells can respond very rapidly to foreign attacks of microbes by releasing potent vasoactive and defence-related molecules into the tissues in local areas. These chemical mediators are released from mast cells systemically in our body during a severe allergic reaction which may result in the clinical picture of anaphylaxis. One of the most important chemical mediators of mast cells is histamine, which can cause ...
In this issue of the International Neurourology Journal, we have published an article reviewing new perspectives on mast cell regulation [4]. It was confirmed in other organs that interleukin (IL) 33 can modulate allergic inflammation. IL-33 is closely related to mast cell activation, which is considered the core of IC pathogenesis. Therefore, it may be possible to regulate mast cell activation by inhibiting IL-33. Promising results have been reported in a number of studies. However, because the reaction of the anti-IL-33 could vary in different organs, we have many challenges to overcome before systemic administration of anti-IL-33 as a therapeutic agent can be implemented. Nevertheless, this is valuable work as it presents a potential therapeutic target for an incurable disease yet unconquered by modern medicine ...
Recurrent or perpetual elevation in blood pressure has been observed in multiple studies and may affect up to 31% of patients with mast cell activation disease (systemic mastocytosis, mast cell activation syndrome/disorder, monoclonal mast cell activation syndrome). Despite this high prevalence, many providers continue to believe that this symptom cannot be inherently from mast cell activation.. A number of mast cell mediators are vasoconstrictors, narrowing the blood vessels and elevating blood pressure. Histamine can both increase and lower blood pressure depending on which receptor it acts upon (H1: hypotension; H2: hypertension).. Several mediators participate in the angiotensin-renin pathway. Angiotensin II, the level of which is largely determined by mast cell mediators like renin, strongly elevates blood pressure. Chymase, involved in the angiotensin-renin pathway, can also either increase or lower blood pressure depending on concentration relative to other mediators present. ...
In 2008 Dr. Afrin started coming to understand that a newly recognized type of mast cell disease, now called mast cell activation syndrome (MCAS), was the underlying diagnosis in many patients he was seeing who were each suffering large assortments - quite different from one patient to the next - of chronic multisystem inflammatory illnesses of unclear cause. Dr. Afrin soon gained experience that MCAS is far more prevalent than the only mast cell disease previously known to medicine (the rare disease of mastocytosis) and that most MCAS patients, once accurately diagnosed, can eventually find significantly helpful medications targeted at the disease. The frequency and magnitude of the improvements Dr. Afrin has seen - even the relief that comes from finally having a unifying diagnosis other than psychosomatism - have spurred him to focus in this area, not only tending to the needs of his patients but also pursuing research to advance our understanding of the disease and helping to educate other ...
In 2008 Dr. Afrin started coming to understand that a newly recognized type of mast cell disease, now called mast cell activation syndrome (MCAS), was the underlying diagnosis in many patients he was seeing who were each suffering large assortments - quite different from one patient to the next - of chronic multisystem inflammatory illnesses of unclear cause. Dr. Afrin soon gained experience that MCAS is far more prevalent than the only mast cell disease previously known to medicine (the rare disease of mastocytosis) and that most MCAS patients, once accurately diagnosed, can eventually find significantly helpful medications targeted at the disease. The frequency and magnitude of the improvements Dr. Afrin has seen - even the relief that comes from finally having a unifying diagnosis other than psychosomatism - have spurred him to focus in this area, not only tending to the needs of his patients but also pursuing research to advance our understanding of the disease and helping to educate other ...
Mast cell activation initiated by antigen-mediated crosslinking of IgE receptors results in stimulated exocytosis of secretory lysosomes in the process known as degranulation. Much has been learned about the molecular mechanisms important for this process, including the critical role of Ca2+ mobilization, but spatio-temporal relationships between stimulated Ca2+ mobilization and granule exocytosis are incompletely understood. Here we use a novel imaging-based method that utilizes fluorescein isothiocyanate (FITC)-dextran as a reporter for granule exocytosis in RBL mast cells and takes advantage of the pH sensitivity of FITC. We demonstrate the selectivity of FITC-dextran, accumulated by fluid phase uptake, as a marker for secretory lysosomes, and we characterize its capacity to delineate different exocytotic events, including full fusion, kiss-and-run transient fusion, and compound exocytosis. Using this method, we find strong dependence of degranulation kinetics on the duration of ...
Im trying to culture rat BMMCs (from Brown Norway rats) using similar culture conditions to those weve used successfully with mouse BMMCs, but with no luck. My current media is pretty standard - RPMI, b-ME, 10% FBS, P/S/glut, sodium pyruvate. rrIl-3 added fresh, tested with and without rSCF. also tested ConA stimulated spleen conditioned media, as a source of IL-3, but my cells seemed to stick to the plate and die faster than previous cultures. I only change out half the media with each feeding, as with mouse BMMCs or the viability decreases. Very few problems with viability - routinely ,90% even after 35 days in culture. My main problem is that all of my cells keep adhering to the plastic - ive looked at the adherent cells by flow and spun some out onto slides and they appear to be monocytes / macs, which i dont want. All the suspension cells dwindle away quite quickly ...
Other names: mast cell protease I; skeletal muscle protease; skin chymotryptic proteinase; mast cell serine proteinase, chymase; skeletal muscle (SK) protease. Comments: In mast cell granules. In peptidase family S1 (trypsin family). Links to other databases: BRENDA, EXPASY, KEGG, MEROPS, Metacyc, PDB, CAS registry number: 97501-92-3. References 1. Woodbury, R.G., Everitt, M. and Neurath, H. Mast cell proteases. Methods Enzymol. 80 (1981) 588-609. [PMID: 7043202]. 2. Powers, J.C., Tanaka, T., Harper, J.W., Minematsu, Y., Barker, L., Lincoln, D., Crumley, K.V., Fraki, J.E., Schechter, N.M., Lazarus, G.G., Nakajima, K., Nakashino, K., Neurath, H. and Woodbury, R.G. Mammalian chymotrypsin-like enzymes. Comparative reactivities of rat mast cell proteases, human and dog skin chymases, and human cathepsin G with peptide 4-nitroanilide substrates and with peptide chloromethyl ketone and sulfonyl fluoride inhibitors. Biochemistry 24 (1985) 2048-2058. [PMID: 3893542]. 3. Johnson, L.A., Moon, K.E. and ...
Our results clearly demonstrate that acute psychological stress induces cardiac mast cell degranulation in 30 min through the local release of CRH, since anti-CRH serum or affinity purified antibody to CRH could neutralize this effect. The same antiserum to CRH used here had previously been shown to block carrageenin-induced skin inflammation (Karalis et al., 1991) and stress-induced dura mast cell degranulation (Theoharides et al., 1995). Pretreatment with the CRHR-1 selective antagonist Antalarmin partially reduced stress-induced mast cell degranulation, implying that CRH receptors are involved. This finding is supported by the fact that CRH receptor mRNA was shown to be expressed in mouse heart (Stenzelet al., 1995). Direct CRHR-mediated mast cell degranulation has recently been demonstrated in rat skin although human leukemic mast cells were shown to express mRNA for CRHR1 (Theoharideset al., 1998). The fact that antalarmin was only a weak inhibitor may be due to its poor solubility or the ...
Upon dermal vibration, patients with vibratory urticaria (VU) experience localized hives and increased histamine levels in serum. These responses are caused by hyperreactivity of skin mast cells to a mechanical stimulus. We found that patients with a familial form of VU harbor a missense substitution in an adhesion G-protein coupled receptor (aGPCR), ADGRE2, that renders mast cells more susceptible to vibration-induced activation. However, the mechanisms of activation of ADGRE2 and how this mutation enhances the ability of mast cells to respond to a mechanical stimulus is not understood. In the present study, we detail the signaling pathways activated through the ADGRE2 receptor upon ligand binding and vibration. Our results provide insights into the structural requirements for ADGE2 activation, how it signals and possible therapeutic targets for patients with VU.. ...
Mast cells are present in the blood, bone marrow and various tissues throughout the body. They originally arise from the bone marrow and migrate to other areas as needed. Rat studies have previously confirmed that stress increases mast cells in the intestine and causes leaky gut. Mast cells seem to have several important functions in the gut including not only immune function but also gut nerve function. Mast cell activation can result in increase gut contractions or decrease gut contractions. A recent study confirms that the stress hormone corticotropin-releasing hormone (CRH) stimulates mast cells in the human colon through receptors present on the mast cells and can trigger their release of chemicals from granules. Increase mast cells are found in association with other inflammatory bowel diseases such as ulcerative colitis and Crohns disease as well as in celiac disease, allergic esophagus or eosinophilic esophagitis, and in post-infectious irritable bowel syndrome (IBS). Mast cells are ...
Ive been debating doing a month summary for all my doctor visits. Nothing detailed. Types of doctors seen and status of visit. Follow-up, new patient, etc. Im leaning towards doing this even though its a lot of work and being this sick is a job enough already. Ive also decided to try keeping a log of my mast cell explosion episodes since starting Xolair and understanding mast cell activation disease (or syndrome) better. Its so damned aggravating that the most information about MCAS on the internet comes from patients. For the Mast cell degranulation attacks (because I think thats probably the best description) Ill note what I assume are triggers, times, meds, and ALL symptoms. If youre reading this, what would you like to hear about?. ...
Only recently recognised, mast cell activation syndrome (MCAS) is a large, prevalent collection of illnesses resulting from mast cells (MCs) which are inappropriately activated but which, in contrast to the (collectively rare) forms of mastocytosis, are not significantly proliferating. Due to the diversity of direct and indirect, local and remote effects of the menagerie of mediators released by MCs, likely due to highly heterogeneous sets of mutations in MC regulatory elements, MCAS typically presents as chronic, persistent or recurrent, waxing/waning or slowly progressive, generally inflammatory multisystem polymorbidity. Initial manifestations often occur in childhood but are non-specific; in fact, virtually all of the syndromes manifestations are non-specific, leading to decades of mysterious illness complicated by incorrect or superficial diagnoses often poorly responsive to empiric therapies. Diagnosis is further challenged in detecting specific biomarkers of MC activation other than ...
TY - JOUR. T1 - FcεRI Signaling in the Modulation of Allergic Response. T2 - Role of Mast Cell-Derived Exosomes. AU - Lecce, Mario. AU - Molfetta, Rosa. AU - Milito, Nadia Domenica. AU - Santoni, Angela. AU - Paolini, Rossella. PY - 2020/7/30. Y1 - 2020/7/30. N2 - Mast cells (MCs) are immune cells that act as environment resident sentinels playing a crucial role in Th2-mediated immune responses, including allergic reactions. Distinguishing features of MCs are the presence of numerous cytoplasmic granules that encapsulate a wide array of preformed bio-active molecules and the constitutive expression of the high affinity receptor of IgE (FcεRI). Upon FcεRI engagement by means of IgE and multivalent antigens, aggregated receptors trigger biochemical pathways that ultimately lead to the release of granule-stored and newly synthesized pro-inflammatory mediators. Additionally, MCs are also able to release exosomes either constitutively or upon stimulation. Exosomes are nanosized vesicles of ...
Mast cells are cells that reside in the connective tissues, especially those vessels and nerves that are closest to the external surfaces (e.g., skin, lungs, nose, mouth). Their primary functions include defense against parasitic infestations, tissue repair, and the formation of new blood vessels (angiogenesis). A tumor consisting of mast cells is called a mastocytoma, or mast cell tumor.
Mast cell degranulation, the release of allergic mediators, is important in allergy, asthma, and parasite defense. Here we demonstrate...
TY - JOUR. T1 - Glycomic analysis of human mast cells, eosinophils and basophils. AU - North, Simon J.. AU - Von Gunten, Stephan. AU - Antonopoulos, Aristotelis. AU - Trollope, Alana. AU - MacGlashan, Donald W.. AU - Jang-Lee, Jihye. AU - Dell, Anne. AU - Metcalfe, Dean D.. AU - Kirshenbaum, Arnold S.. AU - Bochner, Bruce S.. AU - Haslam, Stuart M.. N1 - Funding Information: This work was supported by the Analytical Glycotechnology Core (Core C) of the Consortium for Functional Glycomics (GM 62116), the Swiss National Foundation (PBBEB-113394 to S.G.), the National Institutes of Health (AI 72265 to B.S.B.). PY - 2012/1. Y1 - 2012/1. N2 - In allergic diseases such as asthma, eosinophils, basophils and mast cells, through release of preformed and newly generated mediators, granule proteins and cytokines, are recognized as key effector cells. While their surface protein phenotypes, mediator release profiles, ontogeny, cell trafficking and genomes have been generally explored and compared, there has ...
wrote: Hi Histonetters, I was wondering if anyone knows how to stain paraffin-embedded tissues for a certain Ab (standard IHC), and use toluidine blue to stain mast cells on the same section. Is it at all possible? I emphasize that I would like to use toluidine blue not for counterstaining, but to detect mast cells, and to still be able to see my IHC staining. Glad to recieve any piece of advice. Thanks, Moran -- Moran Elishmereni Department of Pharmacology and Experimental Therapeutics School of Pharmacy, Faculty of Medicine The Hebrew University of Jerusalem POB 12065 Jerusalem 91120, ISRAEL Tel: 972-2-675-8746 Fax: 972-2-675-8144 Email: [email protected] _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. ...
In summary, I would favor catamenial anaphylaxis and not autoimmune progesterone dermatitis, idiopathic anaphylaxis, mast cell disorder or NSAID induced anaphylaxis.. I recently had an exchange with a lovely young woman who found out her mast cell flares during menstruation were kept completely at bay after receiving a diagnosis of catamenial epilepsy and being put on the proper medicines for that condition. I found that extremely interesting so Ill be doing more research into this and will be writing on it in a future post.. As for those of us with definite mast cell disorders who struggle each month through ovulation and menstruation, we have few options. Hormone therapy generally makes us worse so increasing our doses of regular meds or even relying on rescue meds are our main options. For some of us, having a hysterectomy (or oophorectomy) may be the only thing that stops the female cycle MCAS flares but unfortunately, in some cases, it can also trigger us.. There are no easy ...
Mast cells participate in allergy and inflammation by secreting inflammatory mediators such as histamine and proinflammatory cytokines. Flavonoids are naturally occurring molecules with antioxidant, cytoprotective, and antiinflammatory actions. However, effect of flavonoids on the release of histamine and proinflammatory mediator, and their comparative mechanism of action in mast cells were not well defined. Here, we compared the effect of six flavonoids (astragalin, fisetin, kaempferol, myricetin, quercetin, and rutin) on the mast cell-mediated allergic inflammation. Fisetin, kaempferol, myricetin, quercetin, and rutin inhibited IgE or phorbol-12-myristate 13-acetate and calcium ionophore A23187 (PMACI)-mediated histamine release in RBL-2H3 cells. These five flavonoids also inhibited elevation of intracellular calcium. Gene expressions and secretion of proinflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, and IL-8 were assessed in PMACI-stimulated ...
Proteomic-based drug testing is an emerging approach to establish the clinical value and anti-neoplastic potential of multikinase inhibitors. The multikinase inhibitor midostaurin (PKC412) is a promising new agent used to treat patients with advanced systemic mastocytosis (SM). We examined the target interaction profiles and the mast cell (MC)-targeting effects of two pharmacologically relevant midostaurin metabolites, CGP52421 and CGP62221. All three compounds, midostaurin and the two metabolites, suppressed IgE-dependent histamine secretion in basophils and MC with reasonable IC50 values. Midostaurin and CGP62221 also produced growth inhibition and dephosphorylation of KIT in the MC leukemia cell line HMC-1.2, whereas the second metabolite, CGP52421, which accumulates in vivo, showed no substantial effects. Chemical proteomic profiling and drug competition experiments revealed that midostaurin interacts with KIT and several additional kinase targets. The key downstream regulator FES was recognized by
OMALIZUMAB TRIAL. The results of the first of three phase III clinical trials of the effects of omalizumab in patients with chronic idiopathic urticaria were reported online in the New England Journal of Medicine this week.. Urticaria (hives) occurs when mast cells in the skin are triggered to release histamine and other inflammatory mediators stored in cytoplasmic granules (degranulation). In acute urticaria due to allergic reactions, degranulation is triggered when allergen is bound by IgE antibodies attached to FcεRI receptors on the mast cell surface. However, chronic urticaria is not IgE mediated and the mechanism by which mast cells are triggered to degranulate and release histamine is not known.. Omalizumab is a recombinant humanized monoclonal antibody which binds rapidly to circulating IgE, inhibiting its attachment to FcεRI receptors on basophils and mast cells. Expression of FcεRI receptors in basophils is downregulated within 2 weeks, and within 8 weeks there is reduced ...
Mastocytosis, also known as mast cell disease, is an uncommon disorder caused by the accumulation of a normally rare white blood cell type called mast cells. Normal mast cells are involved in fighting certain infections but are most well known as the primary cells that drive allergic responses. When someone has abnormal accumulations of mast cells in the skin, bone marrow, and
Mast cell chymase antibody [CC1] (chymase 1, mast cell) for ELISA, IHC-Fr, IHC-P, WB. Anti-Mast cell chymase mAb (GTX75583) is tested in Human samples. 100% Ab-Assurance.
Mast Cell chymase antibody (chymase 1, mast cell) for IHC-P, WB. Anti-Mast Cell chymase pAb (GTX105829) is tested in Human, Mouse samples. 100% Ab-Assurance.
Human subcutaneous white adipose tissue (SC WAT) increases the expression of beige adipocyte genes in the winter. Studies in rodents suggest that a number of immune mediators are important in the beiging response. Here we studied the seasonal beiging response in SC WAT from lean humans. We measured the gene expression of various immune cell markers and performed multivariate analysis of the gene expression data to identify genes that predict UCP1. IL4 and, unexpectedly, the mast cell marker CPA3 predicted UCP1 gene expression. We therefore investigated the effects of mast cells on UCP1 induction by adipocytes. TIB64 mast cells responded to cold by releasing histamine and IL4, and this medium stimulated UCP1 expression and lipolysis by 3T3-L1 adipocytes. Pharmacological block of mast cell degranulation potently inhibited histamine release by mast cells and inhibited adipocyte UCP1 mRNA induction by conditioned medium. Consistent with this, the histamine receptor antagonist chlorpheniramine ...
Anaphylaxis is a clinical syndrome often presenting as a medical emergency requiring immediate recognition of symptoms, proper treatment and, if possible, the identification and elimination of risk factors. The symptoms of anaphylaxis are mainly determined by chemicals mediators released upon activation of the immune cells. Mast cells which are abundant in cardiovascular tissues, are the main cells activated during anaphylaxis. Human cardiac mast cells have been identified at the site of sarcolemma, within perivascular tissues, in the adventitia of large coronary arteries, and within coronary plaques. Cardiac mast cells display unique immunological and functional features that make them distinct from mast cells in other tissues. Mast cells play a complex role in the development of several pathological processes in the heart. High affinity receptors for IgE (FcṘI) and for C5a anaphylatoxin are involved in development of systemic and cardiac anaphylactic reactions. Furthermore, in myocardial ...
TY - JOUR. T1 - On the mechanism of mast cell formation. Effect of glucocorticoids on the mast cells of normal and thymectomized rats.. AU - Csaba, G.. AU - Surján, L.. AU - Fischer, J.. AU - Kiss, J.. AU - Törö, I.. PY - 1969. Y1 - 1969. UR - http://www.scopus.com/inward/record.url?scp=0014449696&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0014449696&partnerID=8YFLogxK. M3 - Article. C2 - 5792192. AN - SCOPUS:0014449696. VL - 20. SP - 57. EP - 74. JO - Acta Biologica Hungarica. JF - Acta Biologica Hungarica. SN - 0236-5383. IS - 1. ER - ...
Control of Fyn kinase activity and mast cell degranulation is restored by retroviral transduction of Lyn kinase in Lyn-deficient mast cells. (a) Lyn−/− mast
Buku, A; Priceb, JA; Mendlowitzc, M; Masurd, S (2001). "Mast cell degranulating peptide binds to RBL-2H3 mast cell receptors ... The MCD peptide has an immunotoxic effect on mast cells by releasing histamine from these cells. MCD peptide has also been ... Mast cell degranulating (MCD) peptide is a cationic 22-amino acid residue peptide, which is a component of the venom of the ... As a mast cell activator, the MCD peptide evokes large increases in antigen-specific serum immunoglobulin G (IgG) responses. ...
"Mast cells and atopic dermatitis. Stereological quantification of mast cells in atopic dermatitis and normal human skin". Arch ... Giemsa stains the fungus Histoplasma, Chlamydia bacteria, and can be used to identify mast cells. Giemsa's solution is a ... It can be used to study the adherence of pathogenic bacteria to human cells. It differentially stains human and bacterial cells ... It is also used in Wolbachia cell stain in Drosophila melanogaster.[citation needed] Giemsa stain is a classic blood film stain ...
Brown, W. R. and Hardy, M. H. (1989). Mast Cells in Asebia Mouse Skin. Journal of Investigative Dermatology, 93(5): 708. Hardy ... Experimental Cell Research, 46(2): 367-384. Josefowicz, W. J. and Hardy, M. H. (1978). The expression of the gene asebia in the ... Hardy's curiosity-driven research inspired a new generation of stem cell scientists to use hair follicles as "an accessible and ... Hardy, M. H. and Vielkind, U. (1996). Changing patterns of cell adhesion molecules during mouse pelage hair follicle ...
"Distinguishing mast cell and granulocyte differentiation at the single-cell level". Cell Stem Cell. 6 (4): 361-8. doi:10.1016/j ... although there are less than that found in mast cell granules. Mast cells were once thought to be basophils that migrated from ... The mast cell, another granulocyte, is similar in appearance and function. Both cell types store histamine, a chemical that is ... Heneberg P (November 2011). "Mast cells and basophils: trojan horses of conventional lin- stem/progenitor cell isolates". ...
Mast cell studies; conducted on Rana tigrina.. ...
... mast cell activation; keratinocyte adhesion and it is the main regulator of cell migration. Integrin α10β1 preferentially binds ... It is expressed mainly in epithelial cells and leukocytes and expression rate changes due to cell cycle phase. Functions ... It is specific for mesenchymal cells. Functions include: Chondrocyte proliferation and bone growth; regulation of cell ... They control mainly cell proliferation, migration and adhesion, coagulation cascade activation and they affect ECM structure by ...
"Mast Cell Tumour". Genetic Welfare Problems of Companion Animals. ufaw.org.uk: Universities Federation for Animal Welfare. ... The breed is particularly predisposed to mast cell tumours, a cancer of the immune system. Median lifespan was 10.25 years. ...
... they regulate other immune cell functions (e.g., CD4+ T cell, dendritic cell, B cell, mast cell, neutrophil, and basophil ... Basophils Eosinophils Neutrophils Mast cells Except for the mast cells, their names are derived from their staining ... Mast cells are a type of granulocyte that are present in tissues; they mediate host defense against pathogens (e.g., parasites ... Mast cells are also involved in mediating inflammation and autoimmunity as well as mediating and regulating neuroimmune system ...
... degranulation of mast cells, basophils, and eosinophils; and other processes). In a new development in the field of antibody- ... The fragment crystallizable region (Fc region) is the tail region of an antibody that interacts with cell surface receptors ... The Fc region is, therefore, sometimes incorrectly termed the "fragment constant region". Fc binds to various cell receptors ... In this way, it mediates different physiological effects of antibodies (detection of opsonized particles; cell lysis; ...
It inhibits the degranulation of mast cells. It has a quinidine-like anti-arrhythmic effect. It is isolated from the plant ... Tetrandrine has also been shown to inhibit entry of Ebola virus into host cells in vitro and showed therapeutic efficacy ... "The study of anti-tumor effect of Tetrandrine combined with Nedaplatin on human liver cancer cell line 7402". Zhong Yao Cai = ... "Two-pore channels control Ebola virus host cell entry and are drug targets for disease treatment". Science. 347 (6225): 995-998 ...
"Cutaneous Mast Cell Tumors". The Merck Veterinary Manual. 2006. Retrieved 2007-08-21. Modiano J, Breen M, Burnett R, Parker H, ... Frequently seen cancers include lymphoma, melanoma, mast cell tumors (which are considered to be potentially malignant, even ... Boxers and pugs are prone to multiple mast cell tumors. Scottish terriers have eighteen times the risk of mixed breed dogs to ... Inusah S, Thomas R, Avery P, Lindblad-Toh K, Ostrander E, Cutter G, Avery A (2005). "Distinct B-cell and T-cell ...
He found that mast cells form a unique lineage in the hematopoietic cell system. In subsequent studies, neoplastic mast cells ... Criteria and Global Classification of Mast Cell Disorders with Special Reference to Mast Cell Activation Syndromes: A Consensus ... Valent, Peter; Bettelheim, P. (1992). "Cell surface structures on human basophils and mast cells: biochemical and functional ... of Diagnostic Criteria and Therapies in Mastocytosis and one on the Global Classification of Mast Cell Disorders and Mast Cell ...
Stendahl O, Molin L, Lindroth M (1983). "Granulocyte-mediated release of histamine from mast cells. Effect of myeloperoxidase ... Severe side effects may include a decrease in blood cells, red blood cell breakdown especially in those with glucose-6- ... Abnormalities in white blood cell formation, including aplastic anemia, are rare, yet are the cause of the majority of deaths ...
Involvement of mast cells has been reported reflecting a possible immunoallergic aspect to IH. Mattingly suggests that IH may ... Malone, DG; Wilder, RL (Mar 1989). "Participation of synovial mast cells in intermittent hydrarthrosis". Arthritis Rheum. 32 (3 ... Aspirated synovial fluid is usually sterile but will sometimes show elevated cell count (>100 cells/mL) with 50% being ...
Ribatti, Domenico (2018). "The Staining of Mast Cells: A Historical Overview". International Archives of Allergy and Immunology ... the study of diseased cells). Romanowsky-type stains are used to differentiate cells for microscopic examination in ... shades of purple in the chromatin of the cell nucleus and within granules in the cytoplasm of some white blood cells. This ...
The enclosing mesothelium contains macrophages, lymphocytes and mast cells. They are also known as secondary lymphoid organs. ... The milky spots are made up of mesenchymal cells and are covered in a layer of mesothelium. These structures surround the small ... Other structural elements are plasmocytes and various connective tissue cells including reticular and elastic fibres. There is ...
Since 1993, at least 25 papers have been published on the various effects of PEA on mast cells. These cells are often found in ... PEA, as well as its analogue adelmidrol (di-amide derivative of azelaic acid), can both down-regulate mast cells. PEA reduces ... Xanthos DN, Gaderer S, Drdla R, Nuro E, Abramova A, Ellmeier W, Sandkühler J (June 2011). "Central nervous system mast cells in ... Facci L, Dal Toso R, Romanello S, Buriani A, Skaper SD, Leon A (April 1995). "Mast cells express a peripheral cannabinoid ...
Wouters MM, Vicario M, Santos J (2015). "The role of mast cells in functional GI disorders". Gut. 65 (1): 155-168. doi:10.1136/ ...
Hiramatsu, H; Tadokoro, S; Nakanishi, M; Hirashima, N (December 2010). "Latrotoxin-induced exocytosis in mast cells transfected ... So because of the channel and the insertion in the cell membrane the protein makes the cell more permeable to substances that ... The pores formed by α-LTX in the membrane are permeable to Ca2+ and therefore allow an influx of Ca2+ into the cell. This ... The cytosolic α-LTX precursor molecule is released from the cell by means of holocrine secretion where it ends up in the venom ...
Most histamine in the body is generated in granules in mast cells and in white blood cells (leukocytes) called basophils. Mast ... The most important pathophysiologic mechanism of mast cell and basophil histamine release is immunologic. These cells, if ... can be caused by either dysfunctional internal signals from defective mast cells or by the development of clonal mast cell ... By helping T cells to differentiate, the T cells will be less likely to attack the body's own cells, and instead, attack ...
Subramani, T; Rathnavelu, V; Yeap, SK; Alitheen, NB (Feb 2013). "Influence of mast cells in drug-induced gingival overgrowth". ... Hyperplasia refers to an increased number of cells, and hypertrophy refers to an increase in the size of individual cells. As ... neoplasm benign neoplasms, such as fibromas, papillomas and giant cell granulomas malignant neoplasms, such as a carcinoma or ...
Mast cells secrete it during the anaphylactic reaction, inducing inflammation. It can be found in basophils. It induces ...
The further roteins increase the vascular fluid, fibroblasts and promote the ploriferation of mast cells which produce the ... this will increase the production of fibroblasts and mast cells. Lymphatic obstruction due to any cause can increase the amount ... exocytosis of eosinophils and single-cell necrosis of keratinocytes in AGEP and acanthosis and papillomatosis in pustular ...
Another mast cell stabilizer Gastrocrom, a form of cromoglicic acid has also been used to reduce mast cell degranulation.[ ... The bug bites are actually the clumps of mast cells. Doctors can confirm the presence of mast cells by rubbing the baby's skin ... c-kit is a transmembrane protein which, when bound to Mast Cell Growth Factor (MCGF), signals the cell to divide. Mutations in ... These mast cells, when irritated (e.g. by rubbing the skin, heat exposure), produce too much histamine, triggering an allergic ...
It also stains mast cell granules brown. It can be used with live cells. It is also used to stain cartilage in bone specimens, ... Tomov, Nikola; Dimitrov, Nikolay (2017). "Modified Bismarck brown staining for demonstration of soft tissue mast cells" (PDF). ...
... and mast cells. It is also used by certain lymphocytes such as natural killer (NK) cells and cytotoxic T cells, whose main ... via the activation of tyrosine kinases within the cell. The mast cell releases a mixture of compounds, including histamine, ... Antigens interact with IgE molecules already bound to high affinity Fc receptors on the surface of mast cells to induce ... Cytotoxic T cells and NK cells release molecules like perforin and granzymes by a process of directed exocytosis to kill ...
Bulfone-Paus specializes her research in the biology of the immune response, especially cytokines and mast cells. Mast cells ... ATP induces P2X7 receptor-independent cytokine and chemokine expression through P2X1 and P2X3 receptors in murine mast cells- ... Journal of Immunology Retraction Notice Mast Cells Express Novel Functional IL-15 Receptor α Isoforms- Journal of Immunology ... These cell-signaling molecules play a role in the immune response and include interferons and interleukins. In the fall of 2009 ...
Mast cells are one cell type which act to promote scarring. Scarring during healing can create both physical and psychological ... back to their undifferentiated cell lineage also known as mesenchymal cells. It is this area that is known as the blastema ... Proteins and cell surface receptors found in the ECM differ in fetal and adult wound healing. This is due to the early up ... This involves covering of the wound by a layer of epithelial cells known as the wound cap and subsequent innervation of this ...
"Histamine H4 receptor mediates chemotaxis and calcium mobilization of mast cells". J. Pharmacol. Exp. Ther. 305 (3): 1212-21. ...
... called mast cells. Mast cells have antibodies on their surface called immunoglobulin E (IgE). These act as receptors, and if ... The APC then travels to a lymph node, where it presents the displayed allergen to a CD4+ T-cell, or T-helper cell. The T-cell ... When skin is exposed to an allergen, the antigen-presenting cells (APCs) - also known as Langerhans cell or Dermal Dendritic ... the memory t-cells in the skin recognize the antigen and produce cytokines (chemical signals), which cause more T-cells to ...
Segmented basophils and mast cells 0.1% 0.0-0.2 Celloedd. Erythropoietic Pronormoblasts 0.6% 0.2-1.3 ... Gyda bodau dynol, mae Cell goch y gwaed yn cael eu cynhyrchu gan rhan tu mewn i'r mer esgyrn ym mhen esgyrn hir mewn proses a ... macroffabau, sy'n cyfrannu'n sylweddol at gynhyrchiad Cell goch y gwaed, gan eu bod yn mynd a Haearn ar gyfer cynhyrchiad ... Mae'n hysbys fod MSCau yn addasu, yn vitro neu'n vivo, i osteoblastau, chondroctyeau (cell cartilag), have been shown to ...
"Substance P induces TNF-alpha and IL-6 production through NF kappa B in peritoneal mast cells". Biochimica et Biophysica Acta. ... Substance P has been known to stimulate cell growth in normal and cancer cell line cultures,[37] and it was shown that ... on cells (including cancer cells) bestowing upon them mobility.[40] and metastasis.[41] It has been suggested that cancer ... stem cells, white blood cells) in many tissues and organs. SP amplifies or excites most cellular processes.[15][16] ...
Inspired by the skyline of the Colosseum in Rome, the roofs have a skin suspended from two masts. The buildings were inspired ... In his speech he used words such as "cell" and "metabolism" in relation to urban design. The Metabolist movement grew out of ...
158 Cells of the immune system, such as macrophages, mast cells, plasma cells and eosinophils are found scattered in loose ... The cells of connective tissue include fibroblasts, adipocytes, macrophages, mast cells and leucocytes. ... Cells are spread through an extracellular fluid.. *Ground substance - A clear, colorless, and viscous fluid containing ... In hematopoietic and lymphatic tissues, reticular fibers made by reticular cells provide the stroma-or structural support-for ...
輔助性CD4+/TFH(英語:Follicular B helper T cells)/Th3(英語:T helper 3 cell)/Th17(英語:T helper 17 cell)/調節T細胞 ... T細胞(英語:T cell、T淋巴細胞/T lymphocyte)是淋巴細胞的一種,在免疫反應中扮演着重要的角色。T細胞在胸腺內分化成熟,成熟後移居於周圍淋巴組織中。T是「胸腺」(thymus)而不是甲狀腺(thyroid)的英文縮寫。T細胞
顆粒白血球是一類細胞質中包含顆粒體(英語:Granule_(cell biology))的白血球,又因其細胞核形態多樣而稱多形核白血球,(PMN或PML)。術語多形核白血球通常特指最常見的嗜中性顆粒白血球[1]。顆粒白血球由補體調節蛋白調控從骨髓中產生 ... The eosinophil: the cell and its weapons, the cytokines, its locations. Seminars in Respiratory and Critical Care Medicine
Pulendran B, Ono SJ (2008). "A shot in the arm for mast cells". Nat. Med. 14 (5): 489-490. doi:10.1038/nm0508-489. PMID ... Prussin C, Metcalfe DD (2003). "IgE, mast cells, basophils, and eosinophils". J Allergy Clin Immunol. 111 (2 Suppl): S486-94. ... Mast = pitanje, odtod tudi slovensko ime pitanka). Danes jih uvrščamo med celice imunskega sistema. ...
... mast cell - MedlinePlus - mega-HAART - memory T cells - meninges - meningitis - messenger RNA - metabolism - metastasis - MHC ... T suppressor cells - T4 cell - T4 cells (T-helper cells) - T8 cells - Tanner staging - TAT - TB - template - TeachAIDS - ... B-cell lymphoma - B cells - B lymphocytes (B cells) - bactericidal - bacteriostatic - bacterium - baculovirus - baseline - ... cells - CDC National Prevention Information Network (CDC-NPIN) - cell lines - cell-mediated immunity (CMI) - cellular immunity ...
Mast cells. *Microglia. Disorders. The two commonly used categories of white blood cell disorders divide them quantitatively ... T cells: *CD4+ helper T cells: T cells displaying co-receptor CD4 are known as CD4+ T cells. These cells have T-cell receptors ... B cells: releases antibodies and assists activation of T cells. *T cells: *CD4+ Th (T helper) cells: activate and regulate T ... Natural killer cells: virus-infected and tumor cells.. Deeply staining, eccentric. NK-cells and cytotoxic (CD8+) T-cells. Years ...
The inflammatory cells involved include neutrophil granulocytes and macrophages, two types of white blood cells. Those who ... Aleva FE, Voets LW, Simons SO, de Mast Q, van der Ven AJ, Heijdra YF (March 2017). "Prevalence and Localization of Pulmonary ... Several new long-acting agents are under development.[2] Treatment with stem cells is under study.[201] While there is ... Part of this cell response is brought on by inflammatory mediators such as chemotactic factors. Other processes involved with ...
Mast cells[change , change source]. Main article: Mast cell. Mast cells are a type of innate immune cell in connective tissue ... Instead, NK cells destroy compromised host cells, such as tumor cells or virus-infected cells. It recognises such cells by a ... Natural killer cells[change , change source]. Main article: Natural killer cell. Natural killer cells, or NK cells, are a part ... Dendritic cells[change , change source]. Main article: Dendritic cell. Dendritic cells (DC) are phagocytic cells present in ...
পুষ্ট কোষ (Mast cell). *প্রতিরক্ষিকা (Antibodies). *পূরক ব্যবস্থা (Complement system). *বি কোষ (B cell) ...
Dok su neutrofilni granulociti primarna meta za IL-8, postoji relativno širok krug ćelija (endotelne ćelije, makrofage, mast ... Yuan A, Chen JJ, Yao PL, Yang PC (2006). "The role of interleukin-8 in cancer cells and microenvironment interaction". Front. ... 2003). "Neutrophil gelatinase B and chemokines in leukocytosis and stem cell mobilization". Leuk. Lymphoma 43 (2): 233-41. PMID ... human venular endothelial cells store interleukin 8 in Weibel-Palade bodies". J. Exp. Med. 188 (9): 1757-62. PMC 2212526. PMID ...
... ဟာ mast cells နဲ့ basophils လို့ခေါ်တဲ့ အချို့သော သွေးဖြူဥဆဲလ်တွေကို IgE လို့ခေါ်တဲ့ Antibody တစ်မျိုးကနေပြီး အလွန်အမင်းလှ ...
Synthesis by mast cell-fibroblast monolayers during lymphocyte-dependent mast cell proliferation»։ J. Biol. Chem. 257 (15): ... Cell. Biochem. 48 (3): 161-182։ PMID 6757715։ doi:10.1007/BF00421226 *↑ Cox M., Nelson D. (2004)։ Lehninger, Principles of ... Effects of heparin on polymerase chain reaction for blood white cells»։ J. Clin. Lab. Anal. 13 (3): 133-40։ 1999։ PMID 10323479 ... Cell surface heparin sulfate is a receptor for attachment of envelope protein-free retrovirus-like particles and VSV-G ...
Mast cells[edit]. Main article: Mast cell. Mast cells are a type of innate immune cell that reside in connective tissue and in ... Rather, NK cells destroy compromised host cells, such as tumor cells or virus-infected cells, recognizing such cells by a ... Kupffer cells, and mast cells. These cells present receptors contained on the surface or within the cell, named pattern ... Natural killer cells[edit]. Main article: Natural killer cell. Natural killer cells (NK cells) are a component of the innate ...
2003). "beta 1 Integrin-dependent cell adhesion to EMILIN-1 is mediated by the gC1q domain". J. Biol. Chem. 278 (8): 6160-7. ... 2000). "Elastic fiber proteins in the glomerular mesangium in vivo and in cell culture". Kidney Int. 58 (4): 1588-602. doi: ... Cell. Proteomics. 5 (2): 226-33. doi:10.1074/mcp.M500324-MCP200. PMID 16263699.. ... integrin binding involved in cell-matrix adhesion. Cellular component. • collagen trimer. • proteinaceous extracellular matrix ...
Small cell. *. Bell Laboratories Layered. Space-Time (BLAST). *Massive Multiple-input multiple-output (MIMO) ...
... but it is produced also by a broad variety of cell types including lymphoid cells, mast cells, endothelial cells, cardiac ... positive regulation of heterotypic cell-cell adhesion. • negative regulation of mitotic cell cycle. • endothelial cell ... NK cells, neutrophils, mast cells, eosinophils, and neurons.[5] TNFα is a member of the TNF superfamily, consisting of various ... 2007). "TNF Trafficking to Human Mast Cell Granules: Mature Chain-Dependent Endocytosis". The Journal of Immunology. 178 (9): ...
Mast Cell Activation Syndrome»։ Clinical Reviews in Allergy & Immunology 54 (3): 353-365։ June 2018։ PMID 25944644։ doi:10.1007 ... Grimbaldeston MA, Metz M, Yu M, Tsai M, Galli SJ (December 2006). "Effector and potential immunoregulatory roles of mast cells ... mast cell; 7 - newly formed mediators (prostaglandins, leukotrienes, thromboxanes, PAF) ...
"Oxidative activity of ammonium persulfate salt on mast cells and basophils: implication in hairdressers' asthma". Int. Arch. ...
... multifunctional activities on mast cells". Current Drug Targets. Inflammation and Allergy. 2 (3): 224-31. doi:10.2174/ ... Cathelicidins were originally found in neutrophils, but have since been found in many other cells including epithelial cells ... killing by host of symbiont cells. • chronic inflammatory response. • defense response to bacterium. • antimicrobial humoral ... and T cells". The Journal of Experimental Medicine. 192 (7): 1069-74. doi:10.1084/jem.192.7.1069. PMC 2193321 . PMID 11015447. ...
Anti-allergy: mast cell inhibitors. *Anti-glaucoma: adrenergic agonists, beta-blockers, carbonic anhydrase inhibitors/ ... inhaled and systemic corticosteroids, Beta2-adrenergic agonists, anticholinergics, Mast cell stabilizers. Leukotriene ... monoclonal antibodies and cell therapy (for instance, stem-cell therapies). Other ways to classify medicines are by mode of ... and cell therapy (for instance, stem cell therapies). ... Hits from these screens are then tested in cells and then in ...
... on the surface of other kinds of immune cells called mast cells and basophils, which are both involved in the acute ... and activates the sensitized cell. Activated mast cells and basophils undergo a process called degranulation, during which they ... The reaction is usually seen 2-24 hours after the original reaction.[41] Cytokines from mast cells may also play a role in the ... If later exposure to the same allergen occurs, the allergen can bind to the IgE molecules held on the surface of the mast cells ...
... mast cell stabilizers, which inhibit the degranulation of mast cells that is normally triggered by IgE-binding at FcεRI. Long- ... FcεRI is expressed on mast cells, basophils, and the antigen-presenting dendritic cells in both mice and humans. Binding of ... or natural helper cells). Basophils, which share a common haemopoietic progenitor with mast cells, upon the cross-linking of ... and the secretion of several types of type 2 cytokines like IL-3 and Stem Cell Factor (SCF) which both help the mast cells ...
mast cell granule. • Schwann cell microvillus. • Schmidt-Lanterman incisure. • nucleoplasm. • cell projection cytoplasm. • ... The involvement in oxidative stress diseases, cell signal transduction and cell proliferation process endows AKR1B1 the ... cell signal transduction and cell proliferation process including cardiovascular disorders, sepsis, and cancer.[13] ... Sato S, Lin LR, Reddy VN, Kador PF (August 1993). "Aldose reductase in human retinal pigment epithelial cells". Experimental ...
Genes promoted downstream of LSD1 are involved in cancer cell growth and metastasis, and several tumor cells express high ... Mast, N; Charvet, C; Pikuleva, IA; Stout, CD (8 October 2010). "Structural basis of drug binding to CYP46A1, an enzyme that ...
... its wires supported at the same height by masts at its several corners.[1] The main beam is upwards. Above 10 MHz, the loop is ... Small cell. *. Bell Laboratories Layered. Space-Time (BLAST). *Massive Multiple-input multiple-output (MIMO) ...
A 72-metre (236 ft) mast was constructed in 1912. Shortened flag mast now located at the Australian National Maritime Museum ... Navy gaol with 20 cells. Closed in 1943, demolished in 1948 1884-1888. Original End of Island. Reclaimed from Hill spoil. ... Cell door bolted to wall behind Barracks. The only relic remaining of Detention Quarters ...
1986). „Anticoagulantly active heparin-like molecules from mast cell-deficient mice". Am. J. Physiol. 250 (5 Pt 2): H879-888. ... Heparin se obično skladišti unutar sekretornih granula mast ćelija i oslobađa se samo u vaskulaturu na mestima povrede tkiva. ...
... Steven Pirie-Shepherd srps at galactose.mc.duke.edu Thu Oct 19 06:48:45 EST 1995 *Previous message ... Tilo Brunnee (tilo at brunnee.IN-Berlin.DE) wrote: : Hallo! : I attempt to isolate human mast cell heparin proteoglycan from ... mast cells? : - If so, is the heprain sidechain cleaved from the protein core during or : before degranulation? : - Is there ... Is there another source of heparin than mast cells in humans? : - I wonder how commercially available heparin is isolated. That ...
Mast cell activation disorders involve an unusual accumulation of mast cells that release histamine and other mediators for ... Mast cell activation disorders can cause mast cells to release mediators almost anywhere in the body, including the brain, ... Mast cell activation disorders involve an unusual accumulation of mast cells that release histamine and other mediators for ... As explained by the Reflex Sympathetic Dystrophy Syndrome Association, mast cells are a type of white blood cell commonly known ...
IL-33 Exacerbates CIA in ST2−/− Mice Engrafted with WT Mast Cells.. To directly demonstrate a role for mast cells in IL-33- ... 1998) The IL-1 receptor-related T1 antigen is expressed on immature and mature mast cells and on fetal blood mast cell ... 5). Our data also provide mechanisms by which mast cells could promote inflammatory synovitis (Fig. 6). IL-33 induces mast cell ... Such autoantibodies could bind to both low- and high-affinity Fc receptors on mast cells and subsequently trigger mast cell ...
In mast cell cultures, only adenine among cytosine, adenine, adenosine, ADP and ATP dose-dependently suppressed FcɛRI (a high ... Adenine suppresses IgE-mediated mast cell activation.. [Prashanta Silwal, Keuna Shin, Seulgi Choi, Seong Wook Kang, Jin Bong ... We investigated the anti-allergic effect of adenine on IgE-mediated mast cell activation in vitro and passive cutaneous ... Therefore, adenine may be useful for the treatment of mast cell-mediated allergic diseases. Also, the upregulation of adenine ...
Mast cell hyperplasia must be differentiated from metastatic mast cell tumors. Mast cells within the spleen in the absence of a ... Spleen - Hyperplasia, Mast cell in a female B6C3F1/N mouse from a chronic study. Multifocal aggregates of mast cells (arrows) ... Mast cells are relatively large cells with abundant cytoplasm and typically have numerous densely basophilic intracytoplasmic ... Spleen - Hyperplasia, Mast cell in a female B6C3F1/N mouse from a chronic study (higher magnification of Figure 1). Clusters of ...
This study will examine growth factors that promote and inhibit mast cell proliferation resulting in mastocytosis, a disease of ... Regulation of the Proliferation and Survival of Normal and Neoplastic Human Mast Cells. Trial Phase:. N/A. Minimum Age:. N/A. ... Histologic evidence of increased mast cell number by bone marrow and/or skin biopsy.. Must be under the care of a primary care ... regulate mast cell number and survival in patients with mastocytosis, and to explore the. molecular basis of the disease ...
In LAD2 mast cells, both resveratrol and tranilast inhibited degranulation induced by the mast cell activators substance P, IgE ... The effects of resveratrol on mast cell activation were more marked in human primary CD34+-derived mast cells (HuMC), and the ... We investigated the effects of resveratrol on human mast cell activation in comparison to the anti-allergy drug tranilast. ... In conclusion, resveratrol inhibited key aspects of human mast cell activation to physiological stimuli, and was comparable, if ...
Connective tissue mast cells , are probably the easiest to stain with traditional dye technology. , Mucosal mast cells can be ... mast cells , , , ,Hi everyone , , , ,I have used AA1 for mast cells myself, and found it good. But I would , ,ask, why use ICC ... and dermal dendritic cells (CD68 negative) in a series of histiocytomas. Havent noticed many mucosal (epithelial?) mast cells ... Re: mast cells, controls. ,, Previous Message , Next Message ,, From:. Jeff Silverman ,[email protected],. ...
Mast cells are powerful immune modulators of the tissue microenvironment. Within seconds of activation, these cells release a ... Positive and Negative Signals in Mast Cell Activation. Bulfone-Paus, Silvia Univ Manchester, Div Musculoskeletal & Dermatol Sci ... Here, we discuss the biological and clinical relevance of negative and positive signaling modalities that control mast cell ... and adaptor proteins governs mast cell responsiveness to stimuli. ...
Mast cell stabilizers work to prevent allergy cells called mast cells from breaking open and releasing chemicals that help ... Mast Cell Stabilizers. Mast cell stabilizers work to prevent allergy cells called mast cells from breaking open and releasing ... Mast cell stabilizers are not rescue medicines. They work slowly over time, taking two to six weeks to become effective. Mast ... cell stabilizers come in metered dose inhalers and in a solution for nebulizers. They must be taken two to four times a day to ...
Other neoplastic disorders associated with mast cells include mast cell sarcoma and mast cell leukemia. Mast cell activation ... connective tissue-type mast cells and mucosal mast cells. The activities of the latter are dependent on T-cells. Mast cells are ... Mastocytosis is a rare clonal mast cell disorder involving the presence of too many mast cells (mastocytes) and CD34+ mast cell ... Surface markers: cell surface markers of mast cells were discussed in detail by Heneberg, claiming that mast cells may be ...
Mast cell stabilizers are medications used to prevent or control certain allergic disorders. They block mast cell degranulation ... Castillo M, Scott NW, Mustafa MZ, Mustafa MS, Azuara-Blanco A (2015). "Topical antihistamines and mast cell stabilisers for ... Mast cell stabilizer medications include: β2-adrenergic agonists Cromoglicic acid Ketotifen Loratadine Desloratadine ... "Vitamin D contributes to mast cell stabilization". Allergy. 72 (8): 1184-1192. doi:10.1111/all.13110. PMID 27998003. v t e. ...
... cell which, on becoming injured at a site of tissue damage, releases certain chemicals. Some of these cause a restriction of ... mast cell (mahst) n. a large cell in connective tissue. Mast cells contain heparin, histamine, and serotonin, which are ... mast cell A connective tissue cell which, on becoming injured at a site of tissue damage, releases certain chemicals. Some of ... mast cell A Dictionary of Zoology © A Dictionary of Zoology 1999, originally published by Oxford University Press 1999. ...
... experts in this challenging field explore techniques to research these cells from the most practical point of view. Given the ... In Basophils and Mast Cells: Methods and Protocols, ... Generation of Mast Cells from Murine Stem Cell Progenitors ... Basophils and Mast Cells: Methods and Protocols will provide the necessary tools for future research into mast cells and ... Generation of a Human Allergic Mast Cell Phenotype from CD133+ Stem Cells ...
Learn more about grading mast cell tumors here. ... There are three grades of mast cell tumors: Grade I (well- ... Grading Mast Cell Tumors. There are three grades of mast cell tumors: Grade I (well-differentiated), Grade II (moderately ... What are Mast Cell Tumors? Learn about Mast Cell Tumors in dogs and the best course of treatment. ... "Mast Cell Tumors in Dogs." Feb. 12, 2011. (April 30, 2011). http://www.petcancercenter.org/Cancer_Types_mast_cell_dog.html ...
Treatments and Tools for Mast Cell Tumors. Find Mast Cell Tumors information, treatments for Mast Cell Tumors and Mast Cell ... MedHelps Mast Cell Tumors Center for Information, Symptoms, Resources, ... Posts on Mast Cell Tumors. Mass Cell Tumor in Boston Terrier - Cancer in Pets Community ... My 15 yr old cockerpoo was dx w/mast cell ca multiple tumors to many and large to remove wi... ...
Mast Cell Atopic Eczema Food Allergy Sodium Cromoglycate Systemic Mastocytosis These keywords were added by machine and not by ... Bienenstock J (1988) An update on mast cell heterogeneity. J Allergy Clin Immunol 81: 763PubMedCrossRefGoogle Scholar ... In: Pepys J, Edwards AM (eds) The mast cell: its role in health and disease. Pitman Medical, Bath, p 617Google Scholar ... Enerbäck L (1966) Mast cells in rat gastrointestinal mucosa. II. Dye binding and metachromatic properties. Acta Pathol ...
There are three grades of mast cell tumors: Grade I (well-differentiated), Grade II (moderately differentiated) and Grade III ( ...
However, increasing evidence implicates the important role of mast cells in autoimmune disease like rheumatoid arthritis and ... Here we review the current stage of knowledge about mast cells in autoimmune diseases. ... Mast cells are important in innate immune system. They have been appreciated as potent contributors to allergic reaction. ... Interaction among Mast Cells, T Regulatory Cell (Treg), and Th17 Cells. Treg cells are defined as CD4+CD25+FoxP3+ and are known ...
The development of mature mast cells (MCs) from hematopoietic progenitor cells as well as the identification and ... Murine and human mast cell progenitors.. Schmetzer O1, Valentin P1, Church MK1, Maurer M1, Siebenhaar F2. ... Based on the published data, MCs develop either from a committed progenitor or from a common basophil/mast cell precursor. This ... characterization of committed progenitor cells are a current focus of mast cell research. Most published reports in this area ...
... a cell line with homology to mucosal mast cells [80-84]. The D1 cell line is deficient in GM1 gangliosides and in mast cell ... Mast cells, like blood cells, are derived from pluripotent bone marrow hematopoietic stem cells but, unlike blood cells, they ... It is the microenvironment surrounding the mast cells that determines their mature phenotype [1-6]. Mast cells are effector ... S. J. Galli, "New insights into The riddle of the mast cells: microenvironmental regulation of mast cell development and ...
The recruitment of mast cells to sites of angiogenesis is not completely understood. However, once at the site, mast cell ... Mast cells and angiogenesis.. Meininger CJ1, Zetter BR.. Author information. 1. Department of Medical Physiology, ... Much data exists in the literature to suggest a correlation between mast cell accumulation and angiogenesis. This correlation ... Understanding the role of mast cells in angiogenesis may provide avenues for intervening in and manipulating the ...
Types of Mast Cell Tumors. The mast cell tumors may be benign or malignant and forms due to an overgrowth of mast cells. ... Mast Cells in Dogs. The mast cells are part of the immune system and are present on the skin and other tissues in the body. The ... Grades of Mast Cell Tumors. The grading indicates how malignant the tumor is:. *Grade 1 mast cell tumors are considered benign ... Detecting Mast Cell Tumors. Mast cell tumors may be located anywhere on the dogs skin. The tumors may vary in appearance (i.e ...
MCPs reconstitute mast cell compartments in mast cell-deficient KitW-sh/KitW-sh mice. Lethally irradiated KitW-sh/KitW-sh mice ... Harvested cells were stained for c-Kit and FcεRIα to identify mast cells. c-Kit+FcεRIα+ cells (green) were gated, and their ... Mast cells are present in adult mammals in virtually all vascularized tissues (1, 2). Mast cells express on their surface the ... 9). In each experiment, a few mast cells arose from the population of β7+T1/ST2- GMPs. However, the mast cell-generating ...
Mast cell definition is - a granulocyte that occurs especially in connective tissue and has basophilic granules containing ... Learn More about mast cell. Share mast cell Post the Definition of mast cell to Facebook Share the Definition of mast cell on ... Britannica.com: Encyclopedia article about mast cell. Comments on mast cell What made you want to look up mast cell? Please ... Dictionary Entries near mast cell. mastage mastax mast brown mast cell mastectomy master masters ...
When my 4 year old cat was diagnosed with a mast cell tumor inside his ear I was devastated. He had this swelling inside his ... Cutaneous mast cell tumor in cats can be curative with surgery, if there is no metastasis. The prognosis is good in this case. ... When my 4 year old cat was diagnosed with a mast cell tumor inside his ear I was devastated. He had this swelling inside his ...
Cytologic abnormalities of mast cells in MCL. Mast cells from MCL range from mature mast cells (A-D) to more immature cells ... Sequential immunophenotypic analysis of mast cells in a case of systemic mast cell disease evolving to a mast cell leukemia. ... KIT mutation in mast cells and other bone marrow hematopoietic cell lineages in systemic mast cell disorders: a prospective ... Mast-cell leukemia exome sequencing reveals a mutation in the IgE mast-cell receptor beta chain and KIT V654A. Leukemia 2012;26 ...
Symptoms of Mast Cell Tumors in Dogs. Mast cell tumors in dogs generally develop on the skin. They occur when mast cells, a ... Mast cell tumors make up about 20% of all dog skin tumors. Mast cell tumors can occur in dogs of any age, breed or gender, but ... Treating Mast Cell Tumors in Dogs. If your dogs mast cell tumors have not yet spread to other parts of his body, then surgical ... In some dogs with mast cell tumors, mast cells can be found circulating in the blood. ...
... they found more mast cells. A line of mice that lack mature mast cells (KitW-sh/W-sh mice) gained less weight when fed a " ... conclude that mast cells promote the development of diet-induced obesity and diabetes and that agents that prevent mast cell ... find that mast cells may also play a role in obesity and diabetes. Consistent with previous studies, Liu et al. found more ... Noting that mast cells in WAT of obese humans and mice were frequently located near microvessels, the authors hypothesized that ...
... , Cromolyn Sodium Ophthalmic, Crolom, Olopatadine, Patanol, Lodoxamide, Alomide, Ketotifen ... Ocular Mast Cell Stabilizer. Aka: Ocular Mast Cell Stabilizer, Cromolyn Sodium Ophthalmic, Crolom, Olopatadine, Patanol, ...
The recruitment of mast cells to sites of angiogenesis is not completely understood. However, onc … ... Much data exists in the literature to suggest a correlation between mast cell accumulation and angiogenesis. This correlation ... The recruitment of mast cells to sites of angiogenesis is not completely understood. However, once at the site, mast cell ... Mast cells and angiogenesis Semin Cancer Biol. 1992 Apr;3(2):73-9. ...
National Institutes of Health have developed a method that stops allergic reactions by removing a key receptor from mast cells ... IMAGE: Left: mast cells are activated by allergens reacting with IgE bound to IgE receptors on the mast cell surface to trigger ... Cruse and Metcalfe tested their therapy on mast cells in vitro - where it eliminated activation of mast cells by allergen - and ... rendering mast cells unresponsive to IgE-mediated activation. As FcεRIβ expression is restricted to mast cells and basophils, ...
Characteristics of Mast Cells in Mastocytosis. *Mastocytosis. Observational. *National Institute of Allergy and Infectious ... Study of Factors Regulating Mast Cell Proliferation. *Mastocytosis. Observational. *National Institute of Allergy and ... Reduction of mast-cell induced adverse events and symptoms as summarized and calculated from patient's main complaint ... Phase II Midostaurin in Aggressive Systemic Mastocytosis and Mast Cell Leukemia. *Systemic Mastocytosis, Aggressive (ASM) ...
Anti-bFGF antibody was bound to lung MC, basement membrane, endothelial cells, and smooth-muscle cells. Morphometric analysis ... To investigate the role of mast cells (MC) and their fibrogenic growth factors in silicosis, we performed quantitative ... To investigate the role of mast cells (MC) and their fibrogenic growth factors in silicosis, we performed quantitative ... Anti-bFGF antibody was bound to lung MC, basement membrane, endothelial cells, and smooth-muscle cells. Morphometric analysis ...
Including a table of selected mediators produced by or released from mast cells. ... Discover a review of recent developments in mast cells and atherosclerosis. ... mast cell reconstitution protocol in mast cell-deficient mice.. Apparently, mast cell reconstitution in mast cell-deficient ... and here mast cell-deficient mice directly proved that mast cells play a role atherosclerosis (Sun et al., 2007). Mast cell- ...
Mast cells (MCs) influence intercellular communication during inflammation by secreting cytoplasmic granules that contain ...
  • In mast cell cultures, only adenine among cytosine, adenine, adenosine, ADP and ATP dose-dependently suppressed FcɛRI (a high affinity receptor for IgE)-mediated degranulation with a median inhibitory concentration of 1.6mM. (sigmaaldrich.com)
  • In LAD2 mast cells, both resveratrol and tranilast inhibited degranulation induced by the mast cell activators substance P, IgE/anti-IgE, and compound 48/80. (scirp.org)
  • Monomeric immunoglobulin E alone activates cultured human lung mast cells initiating Ca2+ influx, degranulation, arachidonic acid metabolism and cytokine synthesis. (le.ac.uk)
  • Following both IgE-mediated and substance P-mediated activation, PDE3i-pretreated basophils, LAD2 cells, and HuMCs, showed less degranulation than diluent controls, as measured by surface CD63 expression. (eur.nl)
  • In conclusion PDE3 plays a critical role in basophil and mast cell degranulation and therefore its inhibition may be a treatment option in allergic disease. (eur.nl)
  • Involvement of Bruton's tyrosine kinase in FcepsilonRI-dependent mast cell degranulation and cytokine production. (escholarship.org)
  • Consistent with this finding, cultured mast cells derived from the bone marrow cells of xid or btk null mice exhibited mild impairments in degranulation, and more profound defects in the production of several cytokines, upon FcepsilonRI cross-linking. (escholarship.org)
  • Relative hypoxia stimulated mast cell degranulation via transient receptor potential ankyrin 1. (jci.org)
  • Mast cell activation disorders involve an unusual accumulation of mast cells that release histamine and other mediators for inappropriate or unknown reasons and cause inflammation, reports the Journal of Hematology & Oncology. (reference.com)
  • Mast cell activation disorders can cause mast cells to release mediators almost anywhere in the body, including the brain, according to Mastocytosis Society Canada. (reference.com)
  • 2004) Resveratrol inhibits the release of mediators from bone marrow-derived mouse mast cells in vitro. (scirp.org)
  • Mast cells are commonly recognized for their detrimental role in allergic reactions and can, upon activation through the high-affinity receptor for IgE (FcεRI), rapidly produce and secrete many of the mediators responsible for the typical symptoms in urticaria, asthma and rhinitis. (lu.se)
  • Accumulating within inflamed tissues, mast cells produce cytokines and other mediators that may contribute vitally to ongoing inflammation. (biomedcentral.com)
  • Beyond the acute phase of the immune response, mast cells may participate in the response of tissue to injury by means of mediators that promote angiogenesis and fibrosis. (biomedcentral.com)
  • Retroviral transfer of Emt (= Itk/Tsk), Btk's closest relative, also partially improved the ability of btk mutant mast cells to secrete mediators. (escholarship.org)
  • Mast cell activation disorders is a term proposed to cover diseases and conditions related to activation of mast cells and effects of mast cell mediators. (sdu.dk)
  • Mast cells release many inflammatory mediators that play an important role not only in allergic diseases but also in chronic inflammatory diseases, autoimmune diseases, and others. (elsevier.com)
  • In this review, we discuss the ability of mast cells to extend their mechanisms for intercellular communication beyond the release of soluble mediators. (ru.nl)
  • Itchy body welts, also referred to as hives, appear in response to histamine, a chemical that is released by mast cells, which are its major source, accord. (reference.com)
  • Immunologic activation of purified human lung mast cells (HLMC) and basophils with anti-IgE induced histamine release but failed to elicit any changes in cAMP levels. (eurekamag.com)
  • In contrast, histamine release and monophasic rises in cAMP were observed in both rat peritoneal mast cells (RPMC) challenged with concanavalin A (73% enhancement over basal cAMP 20 sec after activation) and a cultured mouse the bone marrow-derived mast cell (PT18 cell line) passively sensitized with dinitrophenol-specific IgE and stimulated with antigen (39% increase above basal at 15 sec). (eurekamag.com)
  • abstract = "Mast cells (MCs) were first described by Paul Ehrlich 1 in his doctoral thesis. (elsevier.com)
  • Equipped with a diversity of surface receptors and effector capabilities, mast cells are sentinels of the immune system, detecting and delivering a first response to invading bacteria and other insults. (biomedcentral.com)
  • Mast cells express surface receptors for IgG, complement, and specific pathogen-associated molecular patterns. (biomedcentral.com)
  • Mast cell hyperplasia must be differentiated from metastatic mast cell tumors. (nih.gov)
  • As most tumors contain inflammatory cell infiltrates, which often include plentiful MCs, the question as to the possible contribution of MCs to tumor development has progressively been emerging. (elsevier.com)
  • Mast cells within the spleen in the absence of a primary mast cell tumor are supportive of a hyperplastic rather than a neoplastic lesion. (nih.gov)
  • In this chapter, the specific involvement of MCs in tumor biology and tumor fate will be considered, with particular emphasis on the capacity of these cells to stimulate tumor growth by promoting angiogenesis and lymphangiogenesis. (elsevier.com)
  • Results confirmed feline cutaneous mast cell tumor. (animalcareinfo.com)
  • A mast cell tumor (MCT) is caused by the over-proliferation of neoplastic mast cells. (animalcareinfo.com)
  • When a patient presents with a skin or subcutaneous mass, it is impossible to eliminate the chance of a patient having a mast cell tumor based on palpation and appearance alone. (animalcareinfo.com)
  • Thus, IL-33 is closely associated with the activation and production of type II cytokines from in vitro polarized Th2 cells ( 1 ). (pnas.org)
  • Mast cells express a high density of ST2 and produce a variety of proinflammatory cytokines in vitro in response to IL-33 ( 1 , 12 - 14 ). (pnas.org)
  • We investigated the anti-allergic effect of adenine on IgE-mediated mast cell activation in vitro and passive cutaneous anaphylaxis (PCA) in mice. (sigmaaldrich.com)
  • As explained by the Reflex Sympathetic Dystrophy Syndrome Association, mast cells are a type of white blood cell commonly known for causing allergic reactions because they release histamines. (reference.com)
  • From this result, we report for the first time that adenine inhibits PCA in mice and allergic reaction by inhibiting FcɛRI-mediated signaling events in mast cells. (sigmaaldrich.com)
  • Therefore, adenine may be useful for the treatment of mast cell-mediated allergic diseases. (sigmaaldrich.com)
  • In this thesis mast cells were, henceforth, studied in human tissue from patients suffering from allergic and non-allergic inflammatory airway diseases. (lu.se)
  • The increase in alveolar mast cell expression of FcεRI might be specific to atopic asthma since we could detect similar changes in mild asthma, but not in patients with allergic rhinitis, CF and COPD. (lu.se)
  • This finding highlights the results of more than 20 years of research indicating that mast cells are frequent participants in non-allergic immune responses as well as in allergy. (biomedcentral.com)
  • Here we review some of the non-allergic functions of mast cells and focus on the potential role of these cells in murine and human inflammatory arthritis. (biomedcentral.com)
  • The mast cell has long been known to mediate important manifestations of allergic disease. (biomedcentral.com)
  • Epithelial mast cells are generally present in the airways of patients with allergic asthma that are inadequately controlled. (eur.nl)
  • Airway mast cells (MCs) are critically involved in allergic airway inflammation and contribute directly to the main symptoms of allergic patients. (eur.nl)
  • Mast cells play a crucial role in the pathogenesis of allergic diseases. (mysciencework.com)
  • These advances have significantly improved our understandings to mast cell biology and to the molecular mechanisms of mast cells in the pathogenesis of allergic diseases. (mysciencework.com)
  • The crosslinking of FcεRI by IgE and antigen (Ag) on mast cells initiates activation cascades that lead to allergic responses. (elsevier.com)
  • The specificity of these effects of btk mutations was confirmed by the improvement in the ability of btk mutant mast cells to degranulate and to secrete cytokines after the retroviral transfer of wild-type btk cDNA, but not of vector or kinase-dead btk cDNA. (escholarship.org)
  • In recent years, tremendous progresses have been made in studies of mast cell origination, migration, proliferation, maturation and survival, and the cytokines regulating these activities. (mysciencework.com)
  • This lesion is characterized by loose aggregates of well-differentiated mast cells within the splenic red pulp that are not associated with inflammation. (nih.gov)
  • 2007) Mast cells in the promotion and limitation of chronic inflammation. (scirp.org)
  • Recent investigations in a murine model have demonstrated that mast cells can have a critical role in the generation of inflammation within the joint. (biomedcentral.com)
  • however, recent studies indicate that these cells also contribute to neovascularization, which is critical for tissue remodeling, chronic inflammation, and carcinogenesis. (jci.org)
  • IL-33 treatment exacerbated CIA in ST2 −/− mice engrafted with mast cells from WT but not from ST2 −/− mice. (pnas.org)
  • However, studies in ST2 −/− mice demonstrate that ST2 is not required for IL-4-induced Th2 cell development or function ( 9 - 11 ), suggesting that ST2/IL-33 may drive type II responses via an alternative pathway or that the function of IL-33/ST2 could extend beyond type II responses. (pnas.org)
  • Correspondingly, mice deficient in mast cells have been found to exhibit striking susceptibility to death from certain types of bacterial infection. (biomedcentral.com)
  • We investigated the role of Bruton's tyrosine kinase (Btk) in FcepsilonRI-dependent activation of mouse mast cells, using xid and btk null mutant mice. (escholarship.org)
  • Unlike B cell development, mast cell development is apparently normal in these btk mutant mice. (escholarship.org)
  • However, mast cells derived from these mice exhibited significant abnormalities in FcepsilonRI-dependent function. (escholarship.org)
  • A lot of mast cells exist in synovium of rheumatoid arthritis, and it is known that synovitis does not occur in mast cell-deficient mice. (elsevier.com)
  • Although mouse strains lacking mast cells did not exhibit retinal neovascularization following hypoxia, these mice developed OIR following infusion of mast cells or after injection of mast cell tryptase (MCT). (jci.org)
  • Mast cell stabilizers as well as specific tryptase and MCP1 inhibitors prevented the development of OIR in WT mice. (jci.org)
  • Within seconds to minutes of IgE crosslinking, granules in the cytoplasm of the mast cell fuse with each other and with the cell surface membrane, ejecting their contents into the extracellular milieu. (biomedcentral.com)
  • Mast cells exist as two different subtypes, MCT (mucosal mast cells) and MCTC (connective tissue mast cells). (lu.se)
  • Since mast cells (unlike the majority of other immune cells) only mature inside the tissue, their final phenotype is highly dependent on the local tissue milieu. (lu.se)
  • Mast cells are found principally in mucosae and in connective tissue, generally clustered at epithelial surfaces and around nerves and blood vessels [ 3 ]. (biomedcentral.com)
  • N2 - BACKGROUND: Lung mast cells are stereotypically divided into connective tissue (MCTC) and mucosal (MCT) mast cells. (lu.se)
  • Recently, a critical role for mast cells in RA biology was proposed ( 26 - 29 ). (pnas.org)
  • B cells are also critically involved in disease pathogenesis by cytokine production, antigen presentation, and autoantibody synthesis that subsequently triggers joint damage either by directly targeting self-tissues or forming immune complexes with autoantigens ( 23 - 25 ). (pnas.org)
  • Mast cells are capable of phagocytosis, intracellular killing, and antigen presentation. (biomedcentral.com)
  • These include formation of mast cell synapses on antigen presenting surfaces, as well as cell-cell contacts with dendritic cells and T cells. (ru.nl)
  • Release of membrane bound exosomes also provide for the transfer of antigen, mast cell proteins, and RNA to other leukocytes. (ru.nl)
  • IL-33 mRNA is broadly expressed in many tissues but is restricted in cellular distribution to smooth muscle cells, epithelial cells, fibroblasts, keratinocytes, dendritic cells, and activated macrophages. (pnas.org)
  • ST2, a member of the IL-1 receptor family, is selectively expressed on Th2 but not Th1 cells and has been implicated in type II but not type I functions ( 5 - 7 ). (pnas.org)
  • Furthermore, both resveratrol and tranilast reduced expression of the high affinity IgE receptor, FcεRI, on LAD2 cells. (scirp.org)
  • A variety of histological methods are used to study the balance between MCT and MCTC, mast cell morphology and mediator and receptor expression patterns in different lung compartments. (lu.se)
  • Monomeric immunoglobulin (Ig)E in the absence of allergen induces mediator release from rodent mast cells, indicating a possible role for IgE in the continued activation of mast cells within the asthmatic bronchial mucosa. (le.ac.uk)
  • Here, we demonstrate that mast cells are essential for sprouting angiogenesis in a murine model of oxygen-induced retinopathy (OIR). (jci.org)
  • Subsequent surges in MCT stimulated retinal endothelial cells to produce monocyte chemotactic protein-1 (MCP1) and angiogenic factors, leading to sprouting angiogenesis. (jci.org)
  • This paper investigates the pathophysiological role and disease-modifying effects of PDE3 in mouse bone marrow-derived MCs (bmMCs), human LAD2- and HMC1 mast cell lines, human blood basophils, and peripheral blood-derived primary human MCs (HuMCs). (eur.nl)
  • Mouse bone marrow-derived MCs (bmMCs) and the human HMC1 and LAD2 cell lines predominantly expressed PDE3B and PDE4A. (eur.nl)
  • We investigated the effects of resveratrol on human mast cell activation in comparison to the anti-allergy drug tranilast. (scirp.org)
  • In conclusion, resveratrol inhibited key aspects of human mast cell activation to physiological stimuli, and was comparable, if not more efficacious than the anti-allergy drug tranilast. (scirp.org)
  • In addition to their central role in allergy, mast cells are involved in a wide variety of cellular interactions during homeostasis and disease. (ru.nl)
  • Yamasaki, S & Saito, T 2008, ' Progress in allergy signal research on mast cells: Signal regulation of multiple mast cell responses through FcεRI ', Journal of Pharmacological Sciences , 巻. (elsevier.com)
  • Th1 responses were considered to predominate within RA synovial T cell subsets and thereby contribute significantly to dysregulated cytokine production ( 19 , 20 ). (pnas.org)
  • Mast cell hyperplasia can be seen within the spleen of rodents. (nih.gov)
  • The mast cell populations differ between human and rodents. (lu.se)
  • Mast cell alterations are present in patients with COPD, CF and IPF, diseases characterised by increases in the density of MCTC with increased expression of pro-inflammatory and pro-fibrotic molecules. (lu.se)
  • Mast cells are present in limited numbers in normal human synovium, but in rheumatoid arthritis and other inflammatory joint diseases this population can expand to constitute 5% or more of all synovial cells. (biomedcentral.com)
  • This observation is suggested to have important implications in unravelling the recently proposed role of mast cells in a variety of pulmonary diseases. (lu.se)
  • These results indicate a new way of controlling mast cells and may therefore be the basis for innovative approaches to the treatment of various diseases related to mast cells. (elsevier.com)
  • Together, these results suggest therapies that suppress mast cell activity should be further explored as a potential option for preventing eye diseases and subsequent blindness induced by neovascularization. (jci.org)
  • Although it was thought that IgE binding to FcεRI is a passive "sensitization", recent reports suggest that IgE actively promotes mast cell survival in the absence of Ag. (elsevier.com)
  • Here, we explored directly the role of IL-33 and mast cells in inflammatory arthritis. (pnas.org)
  • However, recent studies demonstrate that IL-17-producing CD4 + T cells (Th17) play a key pathogenic role in RA ( 21 , 22 ). (pnas.org)
  • However, research over the past two decades has revealed that the role of mast cells is not limited to IgE-mediated immune responses. (biomedcentral.com)
  • We review here the functions of mast cells as a prelude to the discussion of the current state of knowledge about the role of mast cells in murine and human inflammatory arthritis. (biomedcentral.com)
  • Taken together, these results demonstrate an important role for Btk in the full expression of FcepsilonRI signal transduction in mast cells. (escholarship.org)
  • Thus, it is thought that mast cells play a very important role in rheumatoid arthritis pathogenesis. (elsevier.com)
  • With the recognition of the extended role mast cells have during immune modulation, further investigation of the processes in which mast cells are involved is necessary. (ru.nl)
  • Mast cell are found throughout the body, but are especially prominent in tissues that have direct contact with the external milieu such as the skin, gastrointestinal tract and lungs. (lu.se)
  • Mast cells are normally found in the skin and many other tissues such as lymph nodes as part of the immune system. (animalcareinfo.com)
  • The effects of resveratrol on mast cell activation were more marked in human primary CD34+-derived mast cells (HuMC), and the polyphenol was significantly more efficacious than tranilast in these cells. (scirp.org)
  • A main finding was that the alveolar mast cell population had low expression of FcεRI in the healthy human lung. (lu.se)
  • Leicester Research Archive: Activation of human lung mast cells by monomeric immunoglobulin E. (le.ac.uk)
  • Activation of human lung mast cells by monomeric immunoglobulin E. (le.ac.uk)
  • In this study it was investigated whether monomeric IgE induces Ca2+ influx and mediator release from human lung mast cells (HLMC). (le.ac.uk)
  • Novel Site-Specific Mast Cell Subpopulations in the Human Lung. (lu.se)
  • Home Research Outputs Novel Site-Specific Mast Cell Subpopulations in the Human Lu. (lu.se)
  • We used clinical doses of 2-cyano-3-hydroxy-N-(4-trifluoromethylphenyl)-butenamide (A77 1726), which is an active metabolite of leflunomide, and decreased the number of viable human primary mast cells in a concentration-dependent manner. (elsevier.com)
  • A77 1726 dramatically induced apoptosis of human mast cells and inhibited the phosphorylation of Akt, an important survival signal of mast cells, in a concentration-dependent manner. (elsevier.com)
  • Preterm infants with early retinopathy of prematurity had markedly higher plasma MCT levels than age-matched infants without disease, suggesting mast cells contribute to human disease. (jci.org)
  • This direct protocol for differentiation of hES cells, without the need of co-culturing, represents a new and unique model to analyze the function and development of human mast cells. (lifemapsc.com)
  • Molecular regulation of mast cell development and maturation. (mysciencework.com)
  • In this review, the signal regulation for the distinct responses of mast cells through FcεRI are discussed. (elsevier.com)
  • Also, the upregulation of adenine production may provide another mechanism for suppressing mast cell activity especially at inflammatory sites. (sigmaaldrich.com)
  • The mechanism of chronic mast cell activation in asthma is unclear. (le.ac.uk)
  • As an underlying molecular mechanism for the generation of diverse responses through FcεRI, we found that the quantity and the duration of the signal through the FcεRI γ chain (FcRγ) determine different mast cell responses. (elsevier.com)