Mass spectrometry. Medical search

An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.

A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.

A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry.

A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.

A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.

Chromatographic techniques in which the mobile phase is a liquid.

Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.

The systematic study of the complete complement of proteins (PROTEOME) of organisms.

A mass-spectrometric technique that is used for microscopic chemical analysis. A beam of primary ions with an energy of 5-20 kiloelectronvolts (keV) bombards a small spot on the surface of the sample under ultra-high vacuum conditions. Positive and negative secondary ions sputtered from the surface are analyzed in a mass spectrometer in regards to their mass-to-charge ratio. Digital imaging can be generated from the secondary ion beams and their intensity can be measured. Ionic images can be correlated with images from light or other microscopy providing useful tools in the study of molecular and drug actions.

The protein complement of an organism coded for by its genome.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

A mass spectrometric technique that is used for the analysis of a wide range of biomolecules, such as glycoalkaloids, glycoproteins, polysaccharides, and peptides. Positive and negative fast atom bombardment spectra are recorded on a mass spectrometer fitted with an atom gun with xenon as the customary beam. The mass spectra obtained contain molecular weight recognition as well as sequence information.

Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels.

A research technique to measure solvent exposed regions of molecules that is used to provide insight about PROTEIN CONFORMATION.

Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.

Techniques for labeling a substance with a stable or radioactive isotope. It is not used for articles involving labeled substances unless the methods of labeling are substantively discussed. Tracers that may be labeled include chemical substances, cells, or microorganisms.

The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.

Analysis of PEPTIDES that are generated from the digestion or fragmentation of a protein or mixture of PROTEINS, by ELECTROPHORESIS; CHROMATOGRAPHY; or MASS SPECTROMETRY. The resulting peptide fingerprints are analyzed for a variety of purposes including the identification of the proteins in a sample, GENETIC POLYMORPHISMS, patterns of gene expression, and patterns diagnostic for diseases.

The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.

A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy.

Determination, by measurement or comparison with a standard, of the correct value of each scale reading on a meter or other measuring instrument; or determination of the settings of a control device that correspond to particular values of voltage, current, frequency or other output.

Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).

An extraction method that separates analytes using a solid phase and a liquid phase. It is used for preparative sample cleanup before analysis by CHROMATOGRAPHY and other analytical methods.

Deuterium. The stable isotope of hydrogen. It has one neutron and one proton in the nucleus.

The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.

Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)

Concentration or quantity that is derived from the smallest measure that can be detected with reasonable certainty for a given analytical procedure.

Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.

An atom or group of atoms that have a positive or negative electric charge due to a gain (negative charge) or loss (positive charge) of one or more electrons. Atoms with a positive charge are known as CATIONS; those with a negative charge are ANIONS.

An indicator of body density as determined by the relationship of BODY WEIGHT to BODY HEIGHT. BMI=weight (kg)/height squared (m2). BMI correlates with body fat (ADIPOSE TISSUE). Their relationship varies with age and gender. For adults, BMI falls into these categories: below 18.5 (underweight); 18.5-24.9 (normal); 25.0-29.9 (overweight); 30.0 and above (obese). (National Center for Health Statistics, Centers for Disease Control and Prevention)

The systematic identification and quantitation of all the metabolic products of a cell, tissue, organ, or organism under varying conditions. The METABOLOME of a cell or organism is a dynamic collection of metabolites which represent its net response to current conditions.

Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.

Detection of drugs that have been abused, overused, or misused, including legal and illegal drugs. Urine screening is the usual method of detection.

Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.

Analysis based on the mathematical function first formulated by Jean-Baptiste-Joseph Fourier in 1807. The function, known as the Fourier transform, describes the sinusoidal pattern of any fluctuating pattern in the physical world in terms of its amplitude and its phase. It has broad applications in biomedicine, e.g., analysis of the x-ray crystallography data pivotal in identifying the double helical nature of DNA and in analysis of other molecules, including viruses, and the modified back-projection algorithm universally used in computerized tomography imaging, etc. (From Segen, The Dictionary of Modern Medicine, 1992)

Devices for accelerating charged particles in a spiral path by a constant-frequency alternating electric field. This electric field is synchronized with the movement of the particles in a constant magnetic field.

Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)

Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.

Atomic species differing in mass number but having the same atomic number. (Grant & Hackh's Chemical Dictionary, 5th ed)

Use of various chemical separation and extraction methods, such as SOLID PHASE EXTRACTION; CHROMATOGRAPHY; and SUPERCRITICAL FLUID EXTRACTION; to prepare samples for analytical measurement of components.

The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.

A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.

The dynamic collection of metabolites which represent a cell's or organism's net metabolic response to current conditions.

Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.

The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.

Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.

The characteristic 3-dimensional shape of a carbohydrate.

The sum of the weight of all the atoms in a molecule.

A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.

The phenomenon whereby certain chemical compounds have structures that are different although the compounds possess the same elemental composition. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)

A chromatography technique in which the stationary phase is composed of a non-polar substance with a polar mobile phase, in contrast to normal-phase chromatography in which the stationary phase is a polar substance with a non-polar mobile phase.

A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).

Methods for assessing flow through a system by injection of a known quantity of an indicator, such as a dye, radionuclide, or chilled liquid, into the system and monitoring its concentration over time at a specific point in the system. (From Dorland, 28th ed)

Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.

A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of PROTEINS; NUCLEIC ACIDS; and CARBOHYDRATES. (Segen, Dictionary of Modern Medicine, 1992)

Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.

Mixtures of many components in inexact proportions, usually natural, such as PLANT EXTRACTS; VENOMS; and MANURE. These are distinguished from DRUG COMBINATIONS which have only a few components in definite proportions.

The chemical alteration of an exogenous substance by or in a biological system. The alteration may inactivate the compound or it may result in the production of an active metabolite of an inactive parent compound. The alterations may be divided into METABOLIC DETOXICATION, PHASE I and METABOLIC DETOXICATION, PHASE II.

Stable oxygen atoms that have the same atomic number as the element oxygen, but differ in atomic weight. O-17 and 18 are stable oxygen isotopes.

The vapor state of matter; nonelastic fluids in which the molecules are in free movement and their mean positions far apart. Gases tend to expand indefinitely, to diffuse and mix readily with other gases, to have definite relations of volume, temperature, and pressure, and to condense or liquefy at low temperatures or under sufficient pressure. (Grant & Hackh's Chemical Dictionary, 5th ed)

Proteins prepared by recombinant DNA technology.

Separation of a mixture in successive stages, each stage removing from the mixture some proportion of one of the substances, for example by differential solubility in water-solvent mixtures. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.

Spectrophotometric techniques by which the absorption or emmision spectra of radiation from atoms are produced and analyzed.

Proteins found in any species of bacterium.

The process of cleaving a chemical compound by the addition of a molecule of water.

The development and use of techniques and equipment to study or perform chemical reactions, with small quantities of materials, frequently less than a milligram or a milliliter.

Ligand-binding assays that measure protein-protein, protein-small molecule, or protein-nucleic acid interactions using a very large set of capturing molecules, i.e., those attached separately on a solid support, to measure the presence or interaction of target molecules in the sample.

The application of medical knowledge to questions of law.

The first chemical element in the periodic table. It has the atomic symbol H, atomic number 1, and atomic weight [1.00784; 1.00811]. It exists, under normal conditions, as a colorless, odorless, tasteless, diatomic gas. Hydrogen ions are PROTONS. Besides the common H1 isotope, hydrogen exists as the stable isotope DEUTERIUM and the unstable, radioactive isotope TRITIUM.

The pressure at any point in an atmosphere due solely to the weight of the atmospheric gases above the point concerned.

Elements of limited time intervals, contributing to particular results or situations.

The development and use of techniques to study physical phenomena and construct structures in the nanoscale size range or smaller.

A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.

Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

The parts of a macromolecule that directly participate in its specific combination with another molecule.

The rate dynamics in chemical or physical systems.

A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained.

Lipids containing at least one monosaccharide residue and either a sphingoid or a ceramide (CERAMIDES). They are subdivided into NEUTRAL GLYCOSPHINGOLIPIDS comprising monoglycosyl- and oligoglycosylsphingoids and monoglycosyl- and oligoglycosylceramides; and ACIDIC GLYCOSPHINGOLIPIDS which comprises sialosylglycosylsphingolipids (GANGLIOSIDES); SULFOGLYCOSPHINGOLIPIDS (formerly known as sulfatides), glycuronoglycosphingolipids, and phospho- and phosphonoglycosphingolipids. (From IUPAC's webpage)

A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.

The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).

The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.

Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.

Addition of methyl groups. In histo-chemistry methylation is used to esterify carboxyl groups and remove sulfate groups by treating tissue sections with hot methanol in the presence of hydrochloric acid. (From Stedman, 25th ed)

A thiol-containing non-essential amino acid that is oxidized to form CYSTINE.

The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.

The systematic study of the structure and function of the complete set of glycans (the glycome) produced in a single organism and identification of all the genes that encode glycoproteins.

Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.

Methodologies used for the isolation, identification, detection, and quantitation of chemical substances.

Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.

Stable carbon atoms that have the same atomic number as the element carbon, but differ in atomic weight. C-13 is a stable carbon isotope.

Reagents with two reactive groups, usually at opposite ends of the molecule, that are capable of reacting with and thereby forming bridges between side chains of amino acids in proteins; the locations of naturally reactive areas within proteins can thereby be identified; may also be used for other macromolecules, like glycoproteins, nucleic acids, or other.

A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.

The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.

Sequential operating programs and data which instruct the functioning of a digital computer.

The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)

Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.

Organic compounds that have a relatively high VAPOR PRESSURE at room temperature.

An essential amino acid. It is often added to animal feed.

Compounds in which a methyl group is attached to the cyano moiety.

The application of TOXICOLOGY knowledge to questions of law.

Methods for determining interaction between PROTEINS.

Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.

Stable nitrogen atoms that have the same atomic number as the element nitrogen, but differ in atomic weight. N-15 is a stable nitrogen isotope.

Chemical groups containing the covalent disulfide bonds -S-S-. The sulfur atoms can be bound to inorganic or organic moieties.

The chemical and physical integrity of a pharmaceutical product.

Glycosides of GLUCURONIC ACID formed by the reaction of URIDINE DIPHOSPHATE GLUCURONIC ACID with certain endogenous and exogenous substances. Their formation is important for the detoxification of drugs, steroid excretion and BILIRUBIN metabolism to a more water-soluble compound that can be eliminated in the URINE and BILE.

The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.

Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.

The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)

Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)

Proteins which contain carbohydrate groups attached covalently to the polypeptide chain. The protein moiety is the predominant group with the carbohydrate making up only a small percentage of the total weight.

Established cell cultures that have the potential to propagate indefinitely.

The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.

A solventless sample preparation method, invented in 1989, that uses a fused silica fiber which is coated with a stationary phase. It is used for sample cleanup before using other analytical methods.

Changes in the amounts of various chemicals (neurotransmitters, receptors, enzymes, and other metabolites) specific to the area of the central nervous system contained within the head. These are monitored over time, during sensory stimulation, or under different disease states.

Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.

A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)

Derivatives of phosphatidic acid in which the hydrophobic regions are composed of two fatty acids and a polar alcohol is joined to the C-3 position of glycerol through a phosphodiester bond. They are named according to their polar head groups, such as phosphatidylcholine and phosphatidylethanolamine.

A filament-like structure consisting of a shaft which projects to the surface of the SKIN from a root which is softer than the shaft and lodges in the cavity of a HAIR FOLLICLE. It is found on most surfaces of the body.

The identification of selected parameters in newborn infants by various tests, examinations, or other procedures. Screening may be performed by clinical or laboratory measures. A screening test is designed to sort out healthy neonates (INFANT, NEWBORN) from those not well, but the screening test is not intended as a diagnostic device, rather instead as epidemiologic.

Any compound containing one or more monosaccharide residues bound by a glycosidic linkage to a hydrophobic moiety such as an acylglycerol (see GLYCERIDES), a sphingoid, a ceramide (CERAMIDES) (N-acylsphingoid) or a prenyl phosphate. (From IUPAC's webpage)

Salts and esters of gentisic acid.

A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.

The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.

The aggregation of soluble ANTIGENS with ANTIBODIES, alone or with antibody binding factors such as ANTI-ANTIBODIES or STAPHYLOCOCCAL PROTEIN A, into complexes large enough to fall out of solution.

Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.

Placing of a hydroxyl group on a compound in a position where one did not exist before. (Stedman, 26th ed)

A multistage process that includes the determination of a sequence (protein, carbohydrate, etc.), its fragmentation and analysis, and the interpretation of the resulting sequence information.

Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.

A phase transition from liquid state to gas state, which is affected by Raoult's law. It can be accomplished by fractional distillation.

Liquids that dissolve other substances (solutes), generally solids, without any change in chemical composition, as, water containing sugar. (Grant & Hackh's Chemical Dictionary, 5th ed)

A field of biology concerned with the development of techniques for the collection and manipulation of biological data, and the use of such data to make biological discoveries or predictions. This field encompasses all computational methods and theories for solving biological problems including manipulation of models and datasets.

Closed vesicles of fragmented endoplasmic reticulum created when liver cells or tissue are disrupted by homogenization. They may be smooth or rough.

A cell line derived from cultured tumor cells.

Errors in metabolic processes resulting from inborn genetic mutations that are inherited or acquired in utero.

Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.

Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.

Cleavage of proteins into smaller peptides or amino acids either by PROTEASES or non-enzymatically (e.g., Hydrolysis). It does not include Protein Processing, Post-Translational.

Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Mathematical procedure that transforms a number of possibly correlated variables into a smaller number of uncorrelated variables called principal components.

A very strong halogenated derivative of acetic acid. It is used in acid catalyzed reactions, especially those where an ester is cleaved in peptide synthesis.

The covalent bonding of an alkyl group to an organic compound. It can occur by a simple addition reaction or by substitution of another functional group.

Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away, and adjusting the residue to a prescribed standard.

An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (LASER) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum.

The products of chemical reactions that result in the addition of extraneous chemical groups to DNA.

A system for verifying and maintaining a desired level of quality in a product or process by careful planning, use of proper equipment, continued inspection, and corrective action as required. (Random House Unabridged Dictionary, 2d ed)

Large, hoofed mammals of the family EQUIDAE. Horses are active day and night with most of the day spent seeking and consuming food. Feeding peaks occur in the early morning and late afternoon, and there are several daily periods of rest.

Different forms of a protein that may be produced from different GENES, or from the same gene by ALTERNATIVE SPLICING.

The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.

The use of molecularly targeted imaging probes to localize and/or monitor biochemical and cellular processes via various imaging modalities that include RADIONUCLIDE IMAGING; ULTRASONOGRAPHY; MAGNETIC RESONANCE IMAGING; FLUORESCENCE IMAGING; and MICROSCOPY.

Organic compounds containing a carbonyl group in the form -CHO.

An examination of chemicals in the blood.

Oligosaccharides containing two monosaccharide units linked by a glycosidic bond.

Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.

Illegitimate use of substances for a desired effect in competitive sports. It includes humans and animals.

Macromolecular complexes formed from the association of defined protein subunits.

The characteristic three-dimensional shape of a molecule.

The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.

Drugs intended for human or veterinary use, presented in their finished dosage form. Included here are materials used in the preparation and/or formulation of the finished dosage form.

Method for assessing flow through a system by injection of a known quantity of radionuclide into the system and monitoring its concentration over time at a specific point in the system. (From Dorland, 28th ed)

A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.

Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.

Examination of urine by chemical, physical, or microscopic means. Routine urinalysis usually includes performing chemical screening tests, determining specific gravity, observing any unusual color or odor, screening for bacteriuria, and examining the sediment microscopically.

Stable elementary particles having the smallest known negative charge, present in all elements; also called negatrons. Positively charged electrons are called positrons. The numbers, energies and arrangement of electrons around atomic nuclei determine the chemical identities of elements. Beams of electrons are called CATHODE RAYS.

Any compound that contains a constituent sugar, in which the hydroxyl group attached to the first carbon is substituted by an alcoholic, phenolic, or other group. They are named specifically for the sugar contained, such as glucoside (glucose), pentoside (pentose), fructoside (fructose), etc. Upon hydrolysis, a sugar and nonsugar component (aglycone) are formed. (From Dorland, 28th ed; From Miall's Dictionary of Chemistry, 5th ed)

The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.

The removal of a soluble component from a liquid mixture by contact with a second liquid, immiscible with the carrier liquid, in which the component is preferentially soluble. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)

Simple sugars, carbohydrates which cannot be decomposed by hydrolysis. They are colorless crystalline substances with a sweet taste and have the same general formula CnH2nOn. (From Dorland, 28th ed)

Spectrophotometry in the infrared region, usually for the purpose of chemical analysis through measurement of absorption spectra associated with rotational and vibrational energy levels of molecules. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

Techniques used in microbiology.

The homogeneous mixtures formed by the mixing of a solid, liquid, or gaseous substance (solute) with a liquid (the solvent), from which the dissolved substances can be recovered by physical processes. (From Grant & Hackh's Chemical Dictionary, 5th ed)

Electropositive chemical elements characterized by ductility, malleability, luster, and conductance of heat and electricity. They can replace the hydrogen of an acid and form bases with hydroxyl radicals. (Grant & Hackh's Chemical Dictionary, 5th ed)

Methods of comparing two or more samples on the same two-dimensional gel electrophoresis gel.

The measurement of the amplitude of the components of a complex waveform throughout the frequency range of the waveform. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)

A colorless, flammable liquid used in the manufacture of FORMALDEHYDE and ACETIC ACID, in chemical synthesis, antifreeze, and as a solvent. Ingestion of methanol is toxic and may cause blindness.

Drugs used by veterinarians in the treatment of animal diseases. The veterinarian's pharmacological armamentarium is the counterpart of drugs treating human diseases, with dosage and administration adjusted to the size, weight, disease, and idiosyncrasies of the species. In the United States most drugs are subject to federal regulations with special reference to the safety of drugs and residues in edible animal products.

Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.

Organic compounds containing the -CO-NH2 radical. Amides are derived from acids by replacement of -OH by -NH2 or from ammonia by the replacement of H by an acyl group. (From Grant & Hackh's Chemical Dictionary, 5th ed)

Single chains of amino acids that are the units of multimeric PROTEINS. Multimeric proteins can be composed of identical or non-identical subunits. One or more monomeric subunits may compose a protomer which itself is a subunit structure of a larger assembly.

Californium. A man-made radioactive actinide with atomic symbol Cf, atomic number 98, and atomic weight 251. Its valence can be +2 or +3. Californium has medical use as a radiation source for radiotherapy.

Organic silicon derivatives used to characterize hydroxysteroids, nucleosides, and related compounds. Trimethylsilyl esters of amino acids are used in peptide synthesis.

Benzene derivatives that include one or more hydroxyl groups attached to the ring structure.

Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).

The monitoring of the level of toxins, chemical pollutants, microbial contaminants, or other harmful substances in the environment (soil, air, and water), workplace, or in the bodies of people and animals present in that environment.

Compounds containing the -SH radical.

A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.

Formation of an acetyl derivative. (Stedman, 25th ed)

Complex sets of enzymatic reactions connected to each other via their product and substrate metabolites.

Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.

Derivatives of GLUCURONIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that include the 6-carboxy glucose structure.

Hydroxycinnamic acid and its derivatives. Act as activators of the indoleacetic acid oxidizing system, thereby producing a decrease in the endogenous level of bound indoleacetic acid in plants.

A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both.

A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).

Lipid A is the biologically active component of lipopolysaccharides. It shows strong endotoxic activity and exhibits immunogenic properties.

Less common "doping" agents and substances encountered during routine screening for drugs. (1/17751)

The chromatographic and spectroscopic properties of several unusual substances which have been detected in the "alkaloidal" chloroform extract from racehorse urine and saliva samples are reported. Some of these substances have been identified by combined gas chromatography-mass spectrometry and the source of the substance is stated where this is known. Other substances whose identity is not known have been detected and their mass spectra show characteristic amine fragments. The occurrence of these unidentified substances is more frequent in aged urine samples and it would therefore appear that they are associated with putrefaction. (+info)

Urinary lithium: distribution shape, reference values, and evaluation of exposure by inductively coupled plasma argon-emission spectrometry. (2/17751)

Inductively coupled plasma argon-emission spectrometry (ICPAES) was used to evaluate the lithium content of undiluted urine samples. The method can be performed with 1 mL of urine in a single tube using a routine ICPAES analysis for rapid and convenient assessment of lithium exposure in humans. Urine samples obtained from male workers (n = 86) who had not been exposed to lithium were used for the determination of this element by ICPAES. The obtained concentrations were corrected using a specific gravity of 1.024. The particular frequency distribution resulted in a log-normal distribution diagram for anatomical spread. Geometric mean value for urinary lithium in the nonexposed male workers was 23.5 microg/L, and the confidence interval from a log-normal distribution was 11.0 to 50.5 microg/L. Taking into consideration a short biological half-life and the massive urine excretion of lithium, urinary lithium was considered to be a useful index for monitoring of exposure. Calibration curves obtained for lithium standards had good sensitivity and linearity. Good reproducibility was assessed by lithium addition to urine samples. It was concluded that the obtained lithium reference values would be useful for the early diagnosis of lithium intoxication or in the assessment of the degree of exposure to lithium in subjects at risk. (+info)

Archive of mass spectral data files on recordable CD-ROMs and creation and maintenance of a searchable computerized database. (3/17751)

A database containing names of mass spectral data files generated in a forensic toxicology laboratory and two Microsoft Visual Basic programs to maintain and search this database is described. The data files (approximately 0.5 KB/each) were collected from six mass spectrometers during routine casework. Data files were archived on 650 MB (74 min) recordable CD-ROMs. Each recordable CD-ROM was given a unique name, and its list of data file names was placed into the database. The present manuscript describes the use of search and maintenance programs for searching and routine upkeep of the database and creation of CD-ROMs for archiving of data files. (+info)

UV irradiation of polycyclic aromatic hydrocarbons in ices: production of alcohols, quinones, and ethers. (4/17751)

Polycyclic aromatic hydrocarbons (PAHs) in water ice were exposed to ultraviolet (UV) radiation under astrophysical conditions, and the products were analyzed by infrared spectroscopy and mass spectrometry. Peripheral carbon atoms were oxidized, producing aromatic alcohols, ketones, and ethers, and reduced, producing partially hydrogenated aromatic hydrocarbons, molecules that account for the interstellar 3.4-micrometer emission feature. These classes of compounds are all present in carbonaceous meteorites. Hydrogen and deuterium atoms exchange readily between the PAHs and the ice, which may explain the deuterium enrichments found in certain meteoritic molecules. This work has important implications for extraterrestrial organics in biogenesis. (+info)

Relationship between UDP-glucose 4-epimerase activity and oligoglucose glycoforms in two strains of Neisseria meningitidis. (5/17751)

Sodium dodecyl sulfate-polyacrylamide gel analysis of lipooligosaccharide (LOS) from Neisseria meningitidis has demonstrated considerable microheterogeneity in the variable region of LOS due to the presence of novel glycoforms. As a step toward understanding the basis for the expression of these novel glycoforms, we have examined the LOS structures and UDP-glucose 4-epimerase (epimerase) activity levels in two strains (NMB and MA-1) and their respective galE mutants. Strain NMB was found to have low epimerase activity and to contain multiple glycoforms, some of which appear to contain only glucose sugars. The galE mutant had only the oligoglucose glycoforms. Strain MA-1 had higher epimerase activity at both log and stationary phases (2- and 12.5-fold, respectively) and one glycoform with a putative lactosyl structure. Strain MA-1 galE had two glycoforms that contained one or two glucose residues. To understand the molecular basis for the different epimerase activities, we examined the predicted amino acid sequences of the respective galE open reading frames and determined the relative amounts of GalE protein. We found no significant differences between the predicted amino acid sequence of the GalE protein in NMB and that in MA-1. We observed no significant differences in the level of GalE protein between MA-1 and NMB at exponential or stationary phase. We also observed an 8.2-fold drop in epimerase activity in NMB between the log and stationary phases that was not due to the GalE protein level or low glucose levels. (+info)

Insulin-like growth factors I and II are unable to form and maintain their native disulfides under in vivo redox conditions. (6/17751)

Insulin-like growth factor (IGF) I does not quantitatively form its three native disulfide bonds in the presence of 10 mM reduced and 1 mM oxidized glutathione in vitro [Hober, S. et al. (1992) Biochemistry 31, 1749-1756]. In this paper, we show (i) that both IGF-I and IGF-II are unable to form and maintain their native disulfide bonds at redox conditions that are similar to the situation in the secretory vesicles in vivo and (ii) that the presence of protein disulfide isomerase does not overcome this problem. The results indicate that the previously described thermodynamic disulfide exchange folding problem of IGF-I in vitro is also present in vivo. Speculatively, we suggest that the thermodynamic disulfide exchange properties of IGF-I and II are biologically significant for inactivation of the unbound growth factors by disulfide exchange reactions to generate variants destined for rapid clearance. (+info)

Purification and identification of a novel subunit of protein serine/threonine phosphatase 4. (7/17751)

The catalytic subunit of protein serine/threonine phosphatase 4 (PP4C) has greater than 65% amino acid identity to the catalytic subunit of protein phosphatase 2A (PP2AC). Despite this high homology, PP4 does not appear to associate with known PP2A regulatory subunits. As a first step toward characterization of PP4 holoenzymes and identification of putative PP4 regulatory subunits, PP4 was purified from bovine testis soluble extracts. PP4 existed in two complexes of approximately 270-300 and 400-450 kDa as determined by gel filtration chromatography. The smaller PP4 complex was purified by sequential phenyl-Sepharose, Source 15Q, DEAE2, and Superdex 200 gel filtration chromatographies. The final product contained two major proteins: the PP4 catalytic subunit plus a protein that migrated as a doublet of 120-125 kDa on SDS-polyacrylamide gel electrophoresis. The associated protein, termed PP4R1, and PP4C also bound to microcystin-Sepharose. Mass spectrometry analysis of the purified complex revealed two major peaks, at 35 (PP4C) and 105 kDa (PP4R1). Amino acid sequence information of several peptides derived from the 105 kDa protein was utilized to isolate a human cDNA clone. Analysis of the predicted amino acid sequence revealed 13 nonidentical repeats similar to repeats found in the A subunit of PP2A (PP2AA). The PP4R1 cDNA clone engineered with an N-terminal Myc tag was expressed in COS M6 cells and PP4C co-immunoprecipitated with Myc-tagged PP4R1. These data indicate that one form of PP4 is similar to the core complex of PP2A in that it consists of a catalytic subunit and a "PP2AA-like" structural subunit. (+info)

Identification of 17-methyl-18-norandrosta-5,13(17-dien-3beta-ol, the C19 fragment formed by adrenal side chain cleavage of a 20-aryl analog of (20S)-20-hydroxycholesterol. (8/17751)

Incubation of (20R)-20-phenyl-5-pregnene-3beta,20-diol, an aromatic analog of (23S)-20-hydroxycholesterol, with an adrenal mitochondrial preparation leads to the formation of four compounds: pregnenolone, phenol, a C8 ketone, acetophenone, and a nonpolar C19 compound. This latter compound has now been identified by reverse isotope dilution analysis and by gas chromatography/mass spectrometry as 17-methyl-18-norandrosta-5,13(17)-dien-3beta-ol. From these results it is evident that enzymatic fission of the C-17,20 bond of this synthetic derivative occurs. On the other hand, when (20S)-20-hydroxy[21-14C]cholesterol was used as substrate, the analogous cleavage did not take place. Thus, substitution of an aromatic group on C-20 facilitates side chain cleavage between that carbon atom and the nucleus whereas neither of the naturally occuring precursors, cholesterol or its 20-hydroxylated counterpart, are metabolized to a C8 fragment. (+info)

Examples of inborn errors of metabolism include:

1. Phenylketonuria (PKU): A disorder that affects the body's ability to break down the amino acid phenylalanine, leading to a buildup of this substance in the blood and brain.
2. Hypothyroidism: A condition in which the thyroid gland does not produce enough thyroid hormones, leading to developmental delays, intellectual disability, and other health problems.
3. Maple syrup urine disease (MSUD): A disorder that affects the body's ability to break down certain amino acids, leading to a buildup of these substances in the blood and urine.
4. Glycogen storage diseases: A group of disorders that affect the body's ability to store and use glycogen, a form of carbohydrate energy.
5. Mucopolysaccharidoses (MPS): A group of disorders that affect the body's ability to produce and break down certain sugars, leading to a buildup of these substances in the body.
6. Citrullinemia: A disorder that affects the body's ability to break down the amino acid citrulline, leading to a buildup of this substance in the blood and urine.
7. Homocystinuria: A disorder that affects the body's ability to break down certain amino acids, leading to a buildup of these substances in the blood and urine.
8. Tyrosinemia: A disorder that affects the body's ability to break down the amino acid tyrosine, leading to a buildup of this substance in the blood and liver.

Inborn errors of metabolism can be diagnosed through a combination of physical examination, medical history, and laboratory tests such as blood and urine tests. Treatment for these disorders varies depending on the specific condition and may include dietary changes, medication, and other therapies. Early detection and treatment can help manage symptoms and prevent complications.

There are several different types of obesity, including:

1. Central obesity: This type of obesity is characterized by excess fat around the waistline, which can increase the risk of health problems such as type 2 diabetes and cardiovascular disease.
2. Peripheral obesity: This type of obesity is characterized by excess fat in the hips, thighs, and arms.
3. Visceral obesity: This type of obesity is characterized by excess fat around the internal organs in the abdominal cavity.
4. Mixed obesity: This type of obesity is characterized by both central and peripheral obesity.

Obesity can be caused by a variety of factors, including genetics, lack of physical activity, poor diet, sleep deprivation, and certain medications. Treatment for obesity typically involves a combination of lifestyle changes, such as increased physical activity and a healthy diet, and in some cases, medication or surgery may be necessary to achieve weight loss.

Preventing obesity is important for overall health and well-being, and can be achieved through a variety of strategies, including:

1. Eating a healthy, balanced diet that is low in added sugars, saturated fats, and refined carbohydrates.
2. Engaging in regular physical activity, such as walking, jogging, or swimming.
3. Getting enough sleep each night.
4. Managing stress levels through relaxation techniques, such as meditation or deep breathing.
5. Avoiding excessive alcohol consumption and quitting smoking.
6. Monitoring weight and body mass index (BMI) on a regular basis to identify any changes or potential health risks.
7. Seeking professional help from a healthcare provider or registered dietitian for personalized guidance on weight management and healthy lifestyle choices.

... database Mass spectrometry imaging Mass spectrometry software Micro-arrays for mass spectrometry Nanoscale secondary ion mass ... Mass spectrometry Wikimedia Commons has media related to Mass spectrometry. Look up mass spectrometry in Wiktionary, the free ... accelerator mass spectrometry (AMS), thermal ionization-mass spectrometry (TIMS) and spark source mass spectrometry (SSMS). ... Mass spectrometry is often abbreviated as mass-spec or simply as MS. Modern techniques of mass spectrometry were devised by ...

https://en.wikipedia.org/wiki/Mass_spectrometry

... "resolving power in mass spectrometry". doi:10.1351/goldbook.R05321 Biemann, Klaus (1962). Mass Spectrometry: Organic Chemical ... Fundamentals of contemporary mass spectrometry. Chichester: John Wiley & Sons. ISBN 978-0-471-68229-5. (Mass spectrometry). ... In mass spectrometry, resolution is a measure of the ability to distinguish two peaks of slightly different mass-to-charge ... This definition is used in a number of mass spectrometry texts. This use is also implied by the term "high-resolution mass ...

https://en.wikipedia.org/wiki/Resolution_(mass_spectrometry)

... refers to the application of mass spectrometry to the study of proteins. Mass spectrometry is an ... Consequently, protein mass spectrometry now plays a leading role in protein characterization. Mass spectrometry of proteins ... R.E. Iacob & J. R. Engen (2012). "Hydrogen Exchange Mass Spectrometry: Are We Out of the Quicksand?". Mass Spectrometry Reviews ... T. E. Wales & J. R. Engen (2006). "Hydrogen exchange mass spectrometry for the analysis of protein dynamics". Mass Spectrometry ...

https://en.wikipedia.org/wiki/Protein_mass_spectrometry

... (AMS) is a form of mass spectrometry that accelerates ions to extraordinarily high kinetic ... List of accelerator mass spectrometry facilities Arizona Accelerator Mass Spectrometry Laboratory McNaught, A. D.; Wilkinson, A ... de Laeter, J. R. (1998). "Mass spectrometry and geochronology". Mass Spectrometry Reviews. 17 (2): 97-125. Bibcode:1998MSRv... ... Hellborg, Ragnar; Skog, Göran (September 2008). "Accelerator mass spectrometry". Mass Spectrometry Reviews. 27 (5): 398-427. ...

https://en.wikipedia.org/wiki/Accelerator_mass_spectrometry

... is the application of mass spectrometry to the analysis of the composition of aerosol particles. ... Off-line mass spectrometry is performed on collected particles, while on-line mass spectrometry is performed on particles ... Part I: Off-line mass spectrometry techniques". Mass Spectrometry Reviews. 31 (1): 1-16. Bibcode:2012MSRv...31....1P. doi: ... Part II: On-line mass spectrometry techniques". Mass Spectrometry Reviews. 31 (1): 17-48. Bibcode:2012MSRv...31...17P. doi: ...

https://en.wikipedia.org/wiki/Aerosol_mass_spectrometry

... is a mass spectrometry technique that uses multiple stages of tandem mass spectrometry (MSn with n=2 ... site-specific protein N-glycosylations through liquid chromatography/mass spectrometry and targeted tandem mass spectrometry". ... However, the methods of utilizing targeted mass spectrometry are still at a primitive stage, in the sense that the inclusion ... Gillette, Michael A (2013). "Quantitative analysis of peptides and proteins in biomedicine by targeted mass spectrometry". Nat ...

https://en.wikipedia.org/wiki/Targeted_mass_spectrometry

Mass spectrometry/mass spectrometry: techniques and applications of tandem mass spectrometry. New York, N.Y: VCH Publishers. ... Tandem mass spectrometry includes triple quadrupole mass spectrometer (QqQ), multi-sector mass spectrometer, quadrupole-time of ... Mass Spectrometry/Mass Spectrometry: Techniques and Applications of Tandem. Chichester: John Wiley & Sons. ISBN 978-0-471-18699 ... Zaia J (2004). "Mass spectrometry of oligosaccharides". Mass Spectrometry Reviews. 23 (3): 161-227. Bibcode:2004MSRv...23..161Z ...

https://en.wikipedia.org/wiki/Tandem_mass_spectrometry

... "α-Cyano-4-hydroxycinnamic acid as a matrix for matrix-assisted laser desorption mass spectrometry". Organic Mass Spectrometry. ... A new matrix for laser desorption-ionization mass spectrometry". International Journal of Mass Spectrometry and Ion Processes. ... "Detection of 500-nucleotide DNA by laser desorption mass spectrometry". Rapid Communications in Mass Spectrometry. 8 (9): 727- ... "Matrix-assisted laser-desorption mass spectrometry using 355 nm radiation". Rapid Communications in Mass Spectrometry. 3 (12): ...

https://en.wikipedia.org/wiki/Matrix_(mass_spectrometry)

... (MSI) is a technique used in mass spectrometry to visualize the spatial distribution of molecules, as ... McDonnell, Liam A.; Heeren, Ron M.A. (2007-07-01). "Imaging mass spectrometry". Mass Spectrometry Reviews. 26 (4): 606-643. ... McDonnell LA, Heeren RM (2007). "Imaging mass spectrometry". Mass Spectrometry Reviews. 26 (4): 606-43. Bibcode:2007MSRv...26.. ... Imaging Mass Spectrometry Markup Language: A Common Data Format for Mass Spectrometry Imaging in Data Mining in Proteomics: ...

https://en.wikipedia.org/wiki/Mass_spectrometry_imaging

In mass spectrometry the mass defect is defined as the difference between the exact mass and the nearest integer mass. The ... In mass spectrometry, the difference between the nominal mass and the monoisotopic mass is the mass defect. This differs from ... Sleno, Lekha (2012). "The use of mass defect in modern mass spectrometry". Journal of Mass Spectrometry. 47 (2): 226-236. ... The mass defect used in nuclear physics is different from its use in mass spectrometry. In nuclear physics, the mass defect is ...

https://en.wikipedia.org/wiki/Mass_(mass_spectrometry)

Journal of Mass Spectrometry Rapid Communications in Mass Spectrometry John Wiley & Sons v t e (Articles with short description ... Mass Spectrometry Reviews (usually abbreviated as Mass Spectrom. Rev.), is a peer-reviewed scientific journal, published since ... It publishes reviews in selected topics of mass spectrometry and associated scientific disciplines bimonthly. ... Mass spectrometry journals, Publications established in 1987, All stub articles, Biochemistry journal stubs). ...

https://en.wikipedia.org/wiki/Mass_Spectrometry_Reviews

Mass chromatogram Mass spectral interpretation Mass spectrum analysis Tandem mass spectrometry McLafferty FW (1 January 1993). ... "Early gas chromatography/mass spectrometry". Journal of the American Society for Mass Spectrometry. 4 (5): 367-371. doi:10.1021 ... Principles and application of electron-based dissociation in mass spectrometry-based analysis of protein structure" (PDF). Mass ... Tandem mass spectrometry-generated fragmentation is typically made in the collision zone (post-source fragmentation) of a ...

https://en.wikipedia.org/wiki/Fragmentation_(mass_spectrometry)

Liquid chromatography-mass spectrometry Gas chromatography-mass spectrometry Loo JA, Udseth HR, Smith RD (June 1989). "Peptide ... Mass spectrometry is an analytical technique that identifies chemical species depending on their mass-to-charge ratio. During ... and protein analysis by electrospray ionization-mass spectrometry and capillary electrophoresis-mass spectrometry". Analytical ... "Capillary electrophoresis-mass spectrometry as a powerful tool in clinical diagnosis and biomarker discovery". Mass ...

https://en.wikipedia.org/wiki/Capillary_electrophoresis–mass_spectrometry

The British Mass Spectrometry Society. The British Mass Spectrometry Society. Retrieved 16 June 2015. "Summer studentships". ... sustained contributions by individual members of the British Mass Spectrometry Society to the development of mass spectrometry ... The British Mass Spectrometry Society is a registered charity founded in 1964 that encourages participation in every aspect of ... mass spectrometry. It aims to encourage participation in all aspects of mass spectrometry on the widest basis, to promote ...

https://en.wikipedia.org/wiki/British_Mass_Spectrometry_Society

Stable isotope mass spectrometry is conducted in the Department of Geography, and was recently used by the Landmark Trust to ... A Biomolecular Analysis Mass Spectrometry (BAMS) facility was officially opened in 2003, headed by Professor Newton and Dr ... National Mass Spectrometry Service EPSRC National Research Facilities (CS1 maint: url-status, Orphaned articles from December ... In 1975, John H. Beynon was appointed the Royal Society Research Professor and established the Mass Spectrometry Research Unit ...

https://en.wikipedia.org/wiki/Mass_spectrometry_at_Swansea

Capillary electrophoresis-mass spectrometry Ion-mobility spectrometry-mass spectrometry Liquid chromatography-mass spectrometry ... "Gas chromatography-mass spectrometry with supersonic molecular beams". Journal of Mass Spectrometry. 43 (2): 141-63. Bibcode: ... Gas chromatography-mass spectrometry (GC-MS) is an analytical method that combines the features of gas-chromatography and mass ... or in the case of an ion trap MSn where n indicates the number mass spectrometry stages. When a second phase of mass ...

https://en.wikipedia.org/wiki/Gas_chromatography–mass_spectrometry

... (TRMS) is a strategy in analytical chemistry that uses mass spectrometry platform to collect ... "Time-resolved mass spectrometry for monitoring millisecond time-scale solution-phase processes". European Journal of Mass ... Urban P.L., Chen Y.-C., Wang Y.-S. 2016, Time-Resolved Mass Spectrometry: From Concept to Applications. Wiley, Chichester, ISBN ... Meyer, Richard T. (1967). "Flash Photolysis and Time‐Resolved Mass Spectrometry. I. Detection of the Hydroxyl Radical". The ...

https://en.wikipedia.org/wiki/Time-resolved_mass_spectrometry

"Membrane introduction mass spectrometry: trends and applications". Mass Spectrometry Reviews. 19 (1): 1-37. Bibcode:2000MSRv... ... Membrane-introduction mass spectrometry (MIMS) is a method of introducing analytes into the mass spectrometer's vacuum chamber ... v t e (Mass spectrometry, All stub articles, Analytical chemistry stubs). ... Atmospheric pressure chemical ionization Liquid chromatography-mass spectrometry Johnson RC, Cooks RG, Allen TM, Cisper ME, ...

https://en.wikipedia.org/wiki/Membrane-introduction_mass_spectrometry

Gas chromatography-mass spectrometry Capillary electrophoresis-mass spectrometry Ion-mobility spectrometry-mass spectrometry de ... "Mass spectrometry-based plant metabolomics: Metabolite responses to abiotic stress". Mass Spectrometry Reviews. 35 (5): 620-649 ... "Combined liquid chromatography mass spectrometry. Part I. Coupling by means of a moving belt interface". Mass Spectrometry ... "Combined liquid chromatography mass spectrometry. Part III. Applications of thermospray". Mass Spectrometry Reviews. 11 (1): 3- ...

https://en.wikipedia.org/wiki/Liquid_chromatography–mass_spectrometry

... (IAMS) is a form of mass spectrometry that uses a "soft" form of ionization similar to ... "Aromatic ion attachment mass spectrometry: an ion-molecule reaction for organosulfur analysis". Organic Mass Spectrometry. "The ... "Direct analysis of diesel exhaust particles by fragmentation-free mass spectrometry using ion attachment mass spectrometry". ... Fujii T.; Selvin P.C.; Sablier M.; Iwase K. (2001). "Lithium ion attachment mass spectrometry for on-line analysis of trace ...

https://en.wikipedia.org/wiki/Ion-attachment_mass_spectrometry

Society for Mass Spectrometry British Mass Spectrometry Society Canadian Society for Mass Spectrometry List of female mass ... The International Mass Spectrometry Foundation (IMSF) is a non-profit scientific organization in the field of mass spectrometry ... The International Mass Spectrometry Society evolved from this series of International Mass Spectrometry Conferences. The IMSF ... Advances in Mass Spectrometry, which is the oldest continuous series of publications in mass spectrometry. ...

https://en.wikipedia.org/wiki/International_Mass_Spectrometry_Foundation

"chapter 3- Elemental and Isotopic Mass Spectrometry". In Pico, Yolanda (ed.). Advanced mass spectrometry for food safety and ... Thermal ionization mass spectrometry (TIMS) is also known as surface ionization and is a highly sensitive isotope mass ... mass spectrometers with magnetic sectors have better precision than a quadrupole mass spectrometer or quadrupole mass analyzer ... Gross, Jürgen H. (2011). Mass spectrometry : a textbook (2nd ed.). Germany: Springer. ISBN 978-3-642-10711-5. Lehto, J., X. Hou ...

https://en.wikipedia.org/wiki/Thermal_ionization_mass_spectrometry

Ambient Mass-Spectrometry Source". Journal of the American Society for Mass Spectrometry. 25: 800-808. Bibcode:2014JASMS..25.. ... Now in the 2000s the ambient version of flowing afterglow mass spectrometry is flowing atmospheric pressure afterglow mass ... Flowing-afterglow mass spectrometry (FA-MS), is an analytical chemistry technique for the sensitive detection of trace gases. ... doi:10.1016/0020-7381(76)80133-7. Newman K, Mason RS (2006). "Organic mass spectrometry and control of fragmentation using a ...

https://en.wikipedia.org/wiki/Flowing-afterglow_mass_spectrometry

1964 British Mass Spectrometry Society established as first dedicated mass spectrometry society. It holds its first meeting in ... History of Mass Spectrometry - Pioneers - University of New South Wales Sydney Five Mass Spectrometry Nobel Prize Pioneers - ... 2005 Commercialization of Orbitrap MS 2008 ASMS Distinguished Contribution in Mass Spectrometry Award Mass spectrometry History ... Gohlke, R. S.; McLafferty, F. W. (1993). "Early gas chromatography/mass spectrometry". J. Am. Soc. Mass Spectrom. 4 (5): 367- ...

https://en.wikipedia.org/wiki/History_of_mass_spectrometry

Together with the American Society for Mass Spectrometry a JCAMP-DX format for mass spectrometry was developed with aim to ... The Analytical Data Interchange Format for Mass Spectrometry is a format for exchanging data. Many mass spectrometry software ... MGF and PMF Mass spectrometry software Deutsch EW (December 2012). "File formats commonly used in mass spectrometry proteomics ... "An efficient data format for mass spectrometry-based proteomics". Journal of the American Society for Mass Spectrometry. 21 (10 ...

https://en.wikipedia.org/wiki/Mass_spectrometry_data_format

Mass Spectrometry Reviews Rapid Communications in Mass Spectrometry "Journal of Mass Spectrometry". 2020 Journal Citation ... The Journal of Mass Spectrometry is a peer-reviewed scientific journal covering all aspects of mass spectrometry including ... It was established in 1968 as Organic Mass Spectrometry and obtained its current title in 1995. According to the Journal ... Mass spectrometry journals, Publications established in 1995, Wiley (publisher) academic journals, Monthly journals, English- ...

https://en.wikipedia.org/wiki/Journal_of_Mass_Spectrometry

... (IRMS) is a specialization of mass spectrometry, in which mass spectrometric methods are used ... Mass spectrometry of soils. New York: M. Dekker. ISBN 978-0-8247-9699-0. OCLC 34473560. (Geochemistry, Mass spectrometry). ... "High-precision continuous-flow isotope ratio mass spectrometry". Mass Spectrometry Reviews. 16 (5): 227-58. Bibcode:1997MSRv... ... mass spectrometry or infrared spectrometry?". Isotopes in Environmental and Health Studies. 41 (4): 345-61. doi:10.1080/ ...

https://en.wikipedia.org/wiki/Isotope-ratio_mass_spectrometry

Secondary-ion mass spectrometry (SIMS) is a technique used to analyze the composition of solid surfaces and thin films by ... The mass/charge ratios of these secondary ions are measured with a mass spectrometer to determine the elemental, isotopic, or ... a mass analyser separating the ions according to their mass-to-charge ratio, and (5) a detector. SIMS requires a high vacuum ... One was an American project, led by Liebel and Herzog, which was sponsored by NASA at GCA Corp, Massachusetts, for analyzing ...

https://en.wikipedia.org/wiki/Secondary_ion_mass_spectrometry

Downard, Kevin M. (2016). "Indirect study of non-covalent protein complexes by MALDI mass spectrometry: Origins, advantages, ... Kiselar, Janna G.; Downard, Kevin M. (1999). "Antigenic surveillance of the influenza virus by mass spectrometry". Biochemistry ... Mass Spectrometry Reviews. 35: 559-573. doi:10.1002/mas.21480. (Protein-protein interaction assays). ... "Direct Identification of Protein Epitopes by Mass Spectrometry without Immobilization of Antibody and Isolation of Antibody- ...

https://en.wikipedia.org/wiki/Intensity_fading_MALDI_mass_spectrometry

The Canadian Society for Mass Spectrometry is an organization that promotes mass spectrometry in Canada. The goal of the ... "CSMS - Canadian Society for Mass Spectrometry". www.csms-scsm.ca. "International Mass Spectrometry Foundation , Welcome". www. ... The society is an affiliate society of the International Mass Spectrometry Foundation. Its current president is Lekha Sleno. ... Canadian Society for Mass Spectrometry v t e (All articles with dead external links, Articles with dead external links from ...

https://en.wikipedia.org/wiki/Canadian_Society_for_Mass_Spectrometry

... modern gymnosperm resins as well as in the rock record are separated and characterized by gas chromatography-mass spectrometry ... MS-MS analysis has been used to elucidate fragmentation mechanisms for mass spectrum peaks of interest. Mass spectra for ...

https://en.wikipedia.org/wiki/Abietane

... of Chinese star anise fruits and teas for neurotoxic anisatin by Direct Analysis in Real Time high resolution mass spectrometry ...

https://en.wikipedia.org/wiki/Illicium_anisatum

2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. ...

https://en.wikipedia.org/wiki/Caspase-activated_DNase

"Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/ ...

https://en.wikipedia.org/wiki/NOL3

... mass spectrometry, high-field magnetic resonance, and subsurface flow and transport. The Bioproducts, Sciences, and Engineering ... Non-Destructive Analysis Laboratory Radiological Calibration and Irradiation Facility Proteomics other Mass Spectrometry-based ...

https://en.wikipedia.org/wiki/Pacific_Northwest_National_Laboratory

Gas chromatography/mass spectrometry analysis has revealed that A. dracunculus oil contains predominantly phenylpropanoids such ... Louis, MO & Harvard University Herbaria, Cambridge, MA. Lin, Yourun; Humphries, Christopher J.; Gilbert, Michael G. "Artemisia ... Louis, MO & Harvard University Herbaria, Cambridge, MA. "Artemisia dracunculus L.". Flora of Pakistan. Missouri Botanical ...

https://en.wikipedia.org/wiki/Tarragon

2006). "Human Plasma N-Glycoproteome Analysis by Immunoaffinity Subtraction, Hydrazide Chemistry, and Mass Spectrometry". J. ... Liu T, Qian WJ, Gritsenko MA, et al. ( ...

https://en.wikipedia.org/wiki/MUPCDH

Beuhler, R. J.; Flanigan, E.; --; Friedman, L. (1974). "Proton transfer mass spectrometry of peptides. A rapid heating ...

https://en.wikipedia.org/wiki/Lewis_Joel_Greene

Secondary-Ion Mass Spectrometry) and HLE (Hydrogen-Line Extraction) showed this not to be the case. Of particular importance ... electron microprobe and a micro-SIMS for Secondary Ion Mass Spectrometry, and formed a consortium with other local scientists ... Cooper, M.A., Dunning, G.E., Hawthorne, F.C., Ma, C., Kampf, A.R., Spratt, J., Stanley, C.J., Christy, A.G. (2021) Mikecoxite, ...

https://en.wikipedia.org/wiki/Frank_Hawthorne

2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi: ...

https://en.wikipedia.org/wiki/SKIV2L2

The Encyclopedia of Mass Spectrometry: Volume 9: Historical Perspectives, Part B: Notable People in Mass Spectrometry "Advisor ... His professional activities have focused on research and teaching in analytical mass spectrometry, particularly tandem mass ... He won the ASMS Award for Distinguished Contribution to Mass Spectrometry along with Chris Enke in 1993. His research has also ... Yost is currently the president of the American Society for Mass Spectrometry. "www.epi.ufl.edu" (PDF). Retrieved 2019-04-19. " ...

https://en.wikipedia.org/wiki/Richard_Yost

Proteogenomics based approaches utilize information from expressed proteins, often derived from mass spectrometry, to improve ... applications of mass-spectrometry for proteogenomic annotation". Genome Research. 17 (9): 1362-1377. doi:10.1101/gr.6427907. ...

https://en.wikipedia.org/wiki/DNA_annotation

Determination of tetramine in marine gastropods by liquid chromatography/electrospray ionization-mass spectrometry ; Science ...

https://en.wikipedia.org/wiki/Buccinum_leucostoma

... a method of language learning Tandem mass spectrometry, see Mass spectrometry Tandem repeat, a pattern of adjacent repetitions ...

https://en.wikipedia.org/wiki/Tandem_(disambiguation)

... and mass spectrometry". Eur. J. Biochem. 239 (1): 144-9. doi:10.1111/j.1432-1033.1996.0144u.x. PMID 8706699. Kenmochi N, ... Rossman TG, Visalli MA, Komissarova EV (2003). "fau and its ubiquitin-like domain (FUBI) transforms human osteogenic sarcoma ( ... Yu Y, Ji H, Doudna JA, Leary JA (2005). "Mass spectrometric analysis of the human 40S ribosomal subunit: native and HCV IRES- ...

https://en.wikipedia.org/wiki/40S_ribosomal_protein_S30

The concurrent development of mass spectrometry, starting in 1886, supported the concept of atomic and molecular mass and ... and the molar mass of an isotope in grams per mole is equal to the mass number. For example, a molecule of water has a mass of ... 1886: Eugen Goldstein observes discrete particle rays in gas discharges, laying the foundation of mass spectrometry, a tool ... For example, the ratio of the mass of a sample by its amount of substance is the molar mass, whose SI unit is kilograms (or, ...

https://en.wikipedia.org/wiki/Amount_of_substance

... , obtained from an 80 kg sample of Carrara marble, is used as the IAEA-603 isotopic standard in mass spectrometry for ...

https://en.wikipedia.org/wiki/Calcite

Mass spectrometry and mass spectrometry informatics documents have been published as recommendations by the mass spectrometry ... for mass spectrometry data), and ProteoRed MIAPE Generator tool (for gel electrophoresis and mass spectrometry data) It is ... "MIAPE mass spectrometry working group page". HUPO Proteomics Standards Initiative. Retrieved 2009-04-23. "MIAPEGelDB home page ... "Guidelines for reporting the use of mass spectrometry in proteomics". Nat. Biotechnol. 26 (8): 860-861. doi:10.1038/nbt0808-860 ...

https://en.wikipedia.org/wiki/Proteomics_Standards_Initiative

2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi: ...

https://en.wikipedia.org/wiki/RAD54B

International Journal of Mass Spectrometry. 220 (2): 127. Bibcode:2002IJMSp.220..127B. doi:10.1016/S1387-3806(02)00689-9. A.V. ...

https://en.wikipedia.org/wiki/Rydberg_matter

Bottom-up proteomics Mass spectrometry software Protein mass spectrometry Shotgun lipidomics Top-down proteomics Alves P, ... Nesvizhskii AI (2007). "Protein identification by tandem mass spectrometry and sequence database searching". Mass Spectrometry ... Tandem mass spectrometry is then used to identify the peptides. Targeted proteomics using SRM and data-independent acquisition ... As the peptides elute from the column, they are ionized and separated by m/z in the first stage of tandem mass spectrometry. ...

https://en.wikipedia.org/wiki/Shotgun_proteomics

... a mass spectrometry approach". Journal of the American Society for Mass Spectrometry. 21 (9): 1633-42. doi:10.1016/j.jasms. ... the crystallized structure of the catalytic region of TPST-2 and different experiments other methods using mass spectrometry ...

https://en.wikipedia.org/wiki/Tyrosylprotein_sulfotransferase

Ion attachment mass spectrometry (IAMS) can be used to measure BFR concentrations in plastics. The BFR ban has significantly ... REACH Battery Directive Electronic waste Green computing Ion attachment mass spectrometry - used to enforce RoHS limits on ... with full substance declaration by mass. Similarly, good developers (and users) should carefully validate the product info to ...

https://en.wikipedia.org/wiki/Restriction_of_Hazardous_Substances_Directive

"Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. doi: ... Ma X, Zhao H, Shan J, Long F, Chen Y, Chen Y, Zhang Y, Han X, Ma D (Jun 2007). "PDCD10 interacts with Ste20-related kinase MST4 ... "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. doi: ...

https://en.wikipedia.org/wiki/PDCD10

In the 1990s, Laukien was a lecturer in NMR and mass spectrometry at the University of Bremen, Germany, and a part-time ... Laukien was the lead organizer of the Cancer & Evolution Symposium in Boston MA in October 2020. This led to the formation of ... professor at the Institute of Mass Spectrometry of the University of Amsterdam, NL. He has previously served on the Dean's ... In 1984 he earned a bachelor's degree from Massachusetts Institute of Technology, and a Ph.D. in chemical physics from Harvard ...

https://en.wikipedia.org/wiki/Frank_Laukien

... mass spectrometry, and enzyme-linked immunosorbent assay (ELISA), among others, have been used to test for aflatoxin B1 ... Espada Y, Domingo M, Gomez J, Calvo MA (1992). Pathological lesions following an experimental intoxication with aflatoxin B1 in ... Semela, Maryann (2001). "The chemistry and biology of aflatoxin B1: from mutational spectrometry to carcinogenesis" (PDF). ...

https://en.wikipedia.org/wiki/Aflatoxin_B1

"Spectrometry of Primary Charged Particles". NSSDC Master Catalog. NASA. Retrieved 12 September 2011. "Spectrometry of Celestial ... Seven instruments were carried aboard TD-1A, with a combined mass of 120 kilograms (260 lb). The Stellar UV Radiation ... Spectrometry of Celestial X-Rays was another payload operated by the Saclay Nuclear Research Centre. It consisted of two slot ... Spectrometry of Primary Charged Particles was an experiment conducted by the Saclay Nuclear Research Centre, which studied ...

https://en.wikipedia.org/wiki/TD-1A

Chemical analysis done on the Ing'ombe beads using laser ablation inductively coupled plasma mass spectrometry shows that the ...

https://en.wikipedia.org/wiki/Ingombe_Ilede

Affected infants show low levels of free carnitine and all other acylcarnitine species by tandem mass spectrometry. Not all ...

https://en.wikipedia.org/wiki/Systemic_primary_carnitine_deficiency

Wolk demonstrated that mass spectrometry could be used to differentiate biomarkers of anti-microbial resistance as well as in ... "Matrix-assisted laser desorption ionization-time of flight mass spectrometry: a fundamental shift in the routine practice of ...

https://en.wikipedia.org/wiki/Donna_Wolk

Mass spectrometry[edit]. Main article: Mass spectrometry. For coupling to mass spectrometry, the ions from the plasma are ... Inductively coupled plasma mass spectrometry (ICP-MS) is a type of mass spectrometry that uses an inductively coupled plasma to ... compared with other types of mass spectrometry, such as thermal ionization mass spectrometry (TIMS) and glow discharge mass ... This is in contrast to other forms of inorganic mass spectrometry; Glow Discharge Mass Spectrometry (GDMS) and Thermal ...

https://en.wikipedia.org/wiki/Inductively_coupled_plasma_mass_spectrometry

The iCAPTQ is a quadruple-based inductively coupled plasma - mass spectrometer (ICP-MS). This ICP-MS is unique such that up to ... This ICP-MS can operate in 3 different mass resolving modes, including low (,300 m/Δm), medium (,4000 m/Δm) and high (,10,000 m ... The Element 2 is a magnetic sector-based inductively coupled plasma - mass spectrometer (ICP-MS) often referred to as a "high- ... MC-ICP-MS: The NACIL group houses three Multi Collector - Inductively Coupled Plasma - Mass Spectrometer (MC-ICP-MS) systems ...

https://www.ornl.gov/group/nacil/cims/instrumentation

Efficiency of database search for identification of mutated and modified proteins via mass spectrometry. Genome Res. 2001;11(2) ... De novo peptide sequencing via tandem mass spectrometry. J Comput Biol. 1999;6(3-4):327-42. doi:10.1089/106652799318300. ... for multiplexed deep-scale proteome and phosphoproteome analysis of tumor tissues by liquid chromatography-mass spectrometry. ...

https://www.broadinstitute.org/bibcite/keyword/4102

The new technique, called protein mass spectrometry, could uncover other drug targets and reveal how drugs affect cancer cells ... Oregon researchers have used a new mass spectrometry technique to uncover a mutation that can cause leukemia. Their discovery ... who uses mass spectrometry to study cell-signaling networks, is more cautious about direct clinical applications for the mass ... Protein mass spectrometry, in contrast, provides a clearer picture of what the cell is doing. Thats why Cell Signaling ...

https://www.technologyreview.com/2006/08/02/228511/spying-on-cancer-cells-with-mass-spectrometry/

Tandem mass spectrometry (MS/MS). * Gas-phase ion chemistry/physics. * Organic mass spectrometry * Biological mass spectrometry ... Mass spectrometry history. * Isotopes and molecular weight. * Mass analyzers. * Ion sources, ion detectors. * Mass ... Dr Diego Cobice has been working/contributing in the field of Mass Spectrometry (MS) from more than 20 years. He has obtained a ... Finally, he was appointed Director (SO) of Mass Spectrometry Centre at Ulster (BMRSI), where he is currently in charge of ...

https://www.ulster.ac.uk/faculties/life-and-health-sciences/events/2-introduction-to-mass-spectrometry-and-imaging-mass-spectrometry

... tandem mass spectrometry, not tandem mass spectroscopy. * The standard abbreviation for tandem mass spectrometry is MS/MS, not ... mass spectrometry in neonatal screening: a model for clinical mass spectrometry in the next millennium. Acta Paediatr Suppl ... liquid secondary ion mass spectrometry. International Journal of Mass Spectrometry and Ion Processes 1991;111:21128. ... atom bombardment with tandem mass spectrometry and liquid chromatography/mass spectrometry to the analysis of acylcarnitines in ...

https://www.cdc.gov/mmwr/preview/mmwrhtml/rr5003a1.htm

Many faculty in the sciences at Concordia use mass spectrometry in their research. You can check the research interests of ... The Centre for Biological Applications of Mass Spectrometry (CBAMS) is a state-of-the-art facility that supports researchers ... CBAMS takes a leading role in providing Concordia students with hands-on training in the applications of mass spectrometry. ... Centre for Biological Applications of Mass Spectrometry. Concordia University. 7141 Sherbrooke St. W.. Suite SP-180.20. ...

https://www.concordia.ca/research/mass-spec.html

Mass SpectrometryMass Spectrometry *Selective Reagent Ion/Proton transfer reaction - time-of-flight - mass spectrometry (SRI/ ... Mass Spectrometry *Selective Reagent Ion/Proton transfer reaction - time-of-flight - mass spectrometry (SRI/PTR-ToF-MS) *Proton ... Gas Chromatography - Ion Mobility Spectrometry (GC-IMS) *Thermal desorption - gas chromatography - mass spectrometry (TD-GC-MS) ... Gas Chromatography - Ion Mobility Spectrometry (GC-IMS) *Thermal desorption - gas chromatography - mass spectrometry (TD-GC-MS) ...

https://www.ru.nl/tdlab/research/mass_spectrometry/thermal-desorption-gas-chromatography-mass/

RAPID COMMUNICATIONS IN MASS SPECTROMETRY publishes preliminary accounts of recent research in mass spectrometry. ...

https://www.periodicals.com/stock_e/r/ttl10087.html

Clinical Applications of Mass Spectrometry. Clinical Applications of Mass Spectrometry in Barcelona, Spain. Barcelona, Spain. ... Quantitative Mass Spectrometry of Peptides and Proteins, A New Era in Clinical Biomarker Detection Sylvain Lehmann, Professor, ... Innovative Methods and Mass Spectrometry Instrumentation for the Characterization of Proteins and MHC Peptides Donald Hunt, ... Contributions of Mass Spectrometry in the Diagnosis and Management of Hypothalamic-pituitary-adrenal Axis Disorders. Gregori ...

http://selectbiosciences.com/conferences/agenda.aspx?pid=2354&conf=CAMS2014

However, though mass spectrometry is highly accurate and fast, taking only minutes to convert a specimen into a result, it is ... Advancements That Could Bring Proteomics and Mass Spectrometry to Clinical Laboratories. Jan 13, 2020 , Laboratory Instruments ... clinical laboratories find new ways to use mass spectrometry to analyze clinical specimens, producing results that may be more ...

https://www.darkdaily.com/tag/american-society-for-mass-spectrometry/

New Mass Spectrometry Techniques Uncover Clues from Ancient Proteins Researchers can now pinpoint when early cultures began ... and what kind of homemade glue was used to repair a punch bowl owned by George Washingtons mother using new mass spectrometry ...

https://www.aaas.org/disciplines/research-methods/spectroscopy/mass-spectrometry

... In a recent article published in BioTechniques, titled: Democratizing Mass Spectrometry ... In the article Perkel discusses the best way for novices to approach mass spectrometry experiments such as attending courses at ... mass spectrometry is often seen as a complicated and highly technical technique that requires years of experience to master. ... Gas for your mass spec can be provided in various ways; by cylinders, bulk storage or by a gas generator. Traditional gas ...

https://www.peakscientific.com/discover/news/mastering-the-art-of-mass-spectrometry/

A large amount of mass spectrometry data comprehensively provides molecular distributions. In this study, we focus on the ... Mass spectrometry imaging (MSI) allows us to visualize the spatial distribution of molecular components in a sample. ...

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0283966

Discover the effectiveness of MALDI-TOF mass spectrometry for organism identification in the clinical laboratory and explore ... Home / Resources / Webinars / Microbiology Webinars / MALDI-TOF Mass Spectrometry in the Clinical Lab ... MALDI-TOF mass spectrometry has emerged as a rapid, cost-efficient and clinically effective tool for identifying pathogens.1,2 ... Describe MALDI-TOF mass spectrometry technology *Plan an implementation of MALDI-TOF for microorganism identification in the ...

https://www.beckmancoulter.com/en/learning-and-events/webinars/microbiology-webinars/maldi-tof-mass-spectrometry-in-the-lab

A Resonance Ionization Spectroscopy/Resonance Ionization Mass Spectrometry Data Service. IV - Data Sheets for Be, In, Li, K, Rb ... Saloman, E. (1993), A Resonance Ionization Spectroscopy/Resonance Ionization Mass Spectrometry Data Service. IV - Data Sheets ... www.nist.gov/publications/resonance-ionization-spectroscopyresonance-ionization-mass-spectrometry-data-service-iv ...

https://www.nist.gov/publications/resonance-ionization-spectroscopyresonance-ionization-mass-spectrometry-data-service-iv

IDT Oligos are analyzed by ESI mass spectrometry as part of our QC assessment prior to shipment, except those containing mixed ... Is mass spectrometry analysis performed on oligonucleotides before they are shipped? Oligos manufactured by IDT are analyzed by ... ESI mass spectrometry as part of our quality control (QC) assessment prior to shipment, except those containing mixed bases. ... Mass spec traces are available in customer online accounts under "Order History". ...

https://www.idtdna.com/pages/support/faqs/is-mass-spectrometry-analysis-performed-on-oligonucleotides-before-they-are-shipped-

Liquid chromatography mass spectrometry (LC-MS) is shown to provide high discriminating power for the identification of textile ... Analysis of Fiber Dyes By Liquid Chromatography Mass Spectrometry (LC-MS) with Electrospray Ionization: Discriminating Between ... Liquid chromatography mass spectrometry (LC-MS) is shown to provide high discriminating power for the identification of textile ...

https://www.astm.org/jfs2004223.html

... is a promising approach for studying protein-drug interactions native mass spectrometry ... Mass spectrometry (MS) performed under native-like conditions (nMS) ... Mass spectra showing how dissociation constants can be calculated using native mass spectrometry. A concertation series of the ... Marcoux J, Wang SC, Politis A, Reading E, Ma J, Biggin PC, et al. Mass spectrometry reveals synergistic effects of nucleotides ...

https://www.europeanpharmaceuticalreview.com/news/65963/native-mass-spectrometry-drug-development/

... ionization-mass spectrometry and chemometric tools. Download Prime PubMed App to iPhone, iPad, or Android ... AnimalsCattleDiscriminant AnalysisFatsMass SpectrometryMeatQuality ControlSwineTriglycerides ... Ambient mass spectrometry employing direct analysis in real time (DART) ion source for olive oil quality and authenticity ... Authentication of animal fats using direct analysis in real time (DART) ionization-mass spectrometry and chemometric tools.. J ...

https://www.unboundmedicine.com/medline/citation/21526761/Authentication_of_animal_fats_using_direct_analysis_in_real_time__DART__ionization_mass_spectrometry_and_chemometric_tools_

For more information about Cellenions single cell mass spectrometry-based proteomics offering, please visit: www.cellenion.com ... Cellenion Announces Co-Marketing Agreement for Single Cell Mass Spectrometry-Based Proteomics Analysis ... Cellenion Announces Co-Marketing Agreement for Single Cell Mass Spectrometry-Based Proteomics Analysis. ... from single cell isolation and automated sample preparation through to mass spectrometry (MS)-based proteomics analysis on ...

https://news.cision.com/bico-group/r/cellenion-announces-co-marketing-agreement-for-single-cell-mass-spectrometry-based-proteomics-analys,c3453970

... were studied using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The reactions ... diphenylmethane-diisocyanate with polymer polyols as revealed by matrix-assisted laser desorption/ionization mass spectrometry ... diphenylmethane-diisocyanate with polymer polyols as revealed by matrix-assisted laser desorption/ionization mass spectrometry† ...

https://pubs.rsc.org/en/content/articlelanding/2016/RA/C6RA06671B

Analysis of Amphetamine and Methamphetamine in Whole Blood by Solid Phase Extraction and Gas Chromatography - Mass Spectrometry ...

https://www.aafs.org/research/analysis-amphetamine-and-methamphetamine-whole-blood-solid-phase-extraction-and-gas

... mass spectrometry alone and also coupled capillary electrophoresis/electrospray mass spectrometry (CE/ES). Clinically ... The application of capillary electrophoresis and mass spectrometry to clinical and environmental problems.. PhD thesis The Open ... Two techniques of mass spectrometry were used within this thesis, fast atom bombardment and electrospray but only electrospray ... Other work involved using CE, ES and FAI3 mass spectrometry, to develop a new technique to detect diol containing compounds. ...

http://oro.open.ac.uk/19806/

... ... 6-Pyruvoyltetrahydropterin synthase deficiency diagnosed in tandem mass spectrometry-based newborn screening. EMHJ - Eastern ...

https://extranet.who.int/iris/restricted/handle/10665/118540

... we developed an analytical method for the determination of the mass concentration of THC and its metabolites... ... Optimisation of a gas chromatography-mass spectrometry method for the simultaneous determination of tetrahydrocannabinol and ... N. Fuchs, et al., "Optimisation of a gas chromatography-mass spectrometry method for the simultaneous determination of ... "Optimisation of a gas chromatography-mass spectrometry method for the simultaneous determination of tetrahydrocannabinol and ...

https://hrcak.srce.hr/clanak/333588

Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) offers superior mass resolving power and mass ... An enrichment strategy was developed to purify oxidised p53 and top-down FT-ICR mass spectrometry revealed unambiguously that ... Study of the molecular details of p53 redox-regulation using Fourier transform ion cyclotron resonance mass spectrometry ... The primary goal of the work described in this thesis was to employ FT-ICR mass spectrometry to investigate the molecular ...

https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.586211

Identification of Residual Blood Proteins in Ticks by Mass Spectrometry Proteomics Samanthi Wickramasekara*1, Jonas Bunikis†1, ... Identification of Residual Blood Proteins in Ticks by Mass Spectrometry Proteomics. ... Tandem mass spectra of 2 peptides from sheep hemoglobin β-subunit identified in a nymph of an Amblyomma americanum tick. A) ...

https://wwwnc.cdc.gov/eid/content/14/8/1273-f1.htm

...

https://figshare.com/articles/dataset/_Mass_spectrometry_scores_for_selected_of_the_proteins_purified_by_affinity_chromatography_using_region_A_/365108/1

Cross-linking/Mass Spectrometry Combined with Ion Mobility on a timsTOF Pro Instrument for Structural Proteomics. Christian H. ... Cross-linking/Mass Spectrometry Combined with Ion Mobility on a timsTOF Pro Instrument for Structural Proteomics ... Cross-linking/Mass Spectrometry Combined with Ion Mobility on a timsTOF Pro Instrument for Structural Proteomics ... Cross-linking/Mass Spectrometry Combined with Ion Mobility on a timsTOF Pro Instrument for Structural Proteomics ...

https://www.biorxiv.org/content/10.1101/2021.03.26.437136v1.supplementary-material

Experts list the top challenges facing widespread adoption of proteomics in the medical laboratory industry Year-by-year, clinical laboratories find new ways to use mass spectrometry to analyze clinical specimens, producing results that may be more precise than test results produced by other methodologies. (darkdaily.com)
The goal of the partnership is to combine Cellenion's cellenONE® and proteoCHIP TM with Thermo Fisher's TMT multiplexing technologies and Thermo Scientific™ Orbitrap™ mass spectrometers to deliver complete solutions, from single cell isolation and automated sample preparation through to mass spectrometry (MS)-based proteomics analysis on single cells. (cision.com)
With single cell proteomics providing vital information on the state of individual cells within heterogeneous environments, the combination of this sample preparation technology with Thermo Fisher's TMT multiplexing technologies and Orbitrap mass spectrometers will provide researchers with powerful tools to standardize MS-based single cell proteomics workflows. (cision.com)

De novo peptide sequencing via tandem mass spectrometry. (broadinstitute.org)
Tandem mass spectra of 2 peptides from sheep hemoglobin β-subunit identified in a nymph of an Amblyomma americanum tick. (cdc.gov)
Steady-state plasma concentrations of methadone, oxycodone, hydrocodone and tramadol were determined by HPLC tandem mass spectrometry. (medscape.com)
Dr, Victor R. De Jesús setting up a liquid chromatography/tandem mass spectrometry run in the laboratory. (cdc.gov)
Newborn screening for isovaleric acidemia using tandem mass spectrometry: data from 1.6 million newborns. (medlineplus.gov)

Prospective Evaluation of a Matrix-assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry System in a Hospital Clinical Microbiology Laboratory for Identification of Bacteria and Yeasts: A Bench-by-bench Study for Assessing the Impact on Time to Identification and Cost-effectiveness. (beckmancoulter.com)
Comparison of Two Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Methods with Conventional Phenotypic Identification for Routine Identification of Bacteria to the Species Level. (beckmancoulter.com)
A combination of direct analysis in real time (DART) ionization coupled to time-of-flight mass spectrometry (TOFMS) and chemometrics was used for animal fat (lard and beef tallow) authentication. (unboundmedicine.com)
Urinary benzo[a]pyrene and its metabolites as molecular biomarkers of asphalt fume exposure characterized by microflow LC coupled to hybrid quadrupole time-of-flight mass spectrometry. (cdc.gov)
This method is based on microflow liquid chromatography (LC) coupled to hybrid quadrupole orthogonal acceleration time-of-flight mass spectrometry (Q-TOFMS). (cdc.gov)
In October 2022, Zimbabwe made the Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) technology available for the clinical microbiology laboratory's quick and precise identification of bacteria, mycobacteria, and fungal infections. (who.int)

[2] The Inductively Coupled Plasma Mass Spectrometry is operated in the H mode. (wikipedia.org)
What makes Inductively Coupled Plasma Mass Spectrometry (ICP-MS) unique to other forms of inorganic mass spectrometry is its ability to sample the analyte continuously, without interruption. (wikipedia.org)
Title : Rapid determination of thorium in urine by quadrupole inductively coupled plasma mass spectrometry (Q-ICP-MS) Personal Author(s) : Liu, Yongzhong;Xiao, Ge;Jones, Robert L. (cdc.gov)

However, compared with other types of mass spectrometry, such as thermal ionization mass spectrometry (TIMS) and glow discharge mass spectrometry (GD-MS), ICP-MS introduces many interfering species: argon from the plasma, component gases of air that leak through the cone orifices, and contamination from glassware and the cones. (wikipedia.org)
Glow Discharge Mass Spectrometry (GDMS) and Thermal Ionization Mass Spectrometry (TIMS), that require a two-stage process: Insert sample(s) into a vacuum chamber, seal the vacuum chamber, pump down the vacuum, energize sample, thereby sending ions into the mass analyzer. (wikipedia.org)
A Resonance Ionization Spectroscopy/Resonance Ionization Mass Spectrometry Data Service. (nist.gov)
TY - JOUR T1 - Authentication of animal fats using direct analysis in real time (DART) ionization-mass spectrometry and chemometric tools. (unboundmedicine.com)
To perform MS-SG, natural libraries of glycans derived from glycoconjugates in cells or tissues are screened against a target GBP using catch-and-release electrospray ionization mass spectrometry ( CaR -ESI-MS). Because glycan concentrations are challenging to determine, ligand affinities cannot be directly measured. (bvsalud.org)

Samples were analysed using a combination of CE alone, mass spectrometry alone and also coupled capillary electrophoresis/electrospray mass spectrometry (CE/ES). (open.ac.uk)

Reproducible workflow for multiplexed deep-scale proteome and phosphoproteome analysis of tumor tissues by liquid chromatography-mass spectrometry. (broadinstitute.org)
Gas chromatography - mass spectrometry (GC-MS) is the golden standard for the analysis of (semi) volatile organic compounds (VOCs). (ru.nl)
Liquid chromatography mass spectrometry (LC-MS) is shown to provide high discriminating power for the identification of textile dyes that can not be reliably distinguished on the basis of their UV/visible absorption profile. (astm.org)
2019). 'Optimisation of a gas chromatography-mass spectrometry method for the simultaneous determination of tetrahydrocannabinol and its metabolites in rat urine', Arhiv za higijenu rada i toksikologiju , 70(4), str. (srce.hr)
For this purpose, hydrolysis and solid phase extraction conditions of the investigated analytes were optimised and a gas chromatography-mass spectrometry (GC-MS) method was developed to determine all three analytes in rat urine. (srce.hr)
The analytical methods used to measure benzidine and its metabolites in biological materials are GC (NIOSH 1984b), gas chromatography/mass spectrometry (GC/MS) (Hsu et al. (cdc.gov)

Efficiency of database search for identification of mutated and modified proteins via mass spectrometry. (broadinstitute.org)
In proteomic mass spectrometry the researchers first break up cancer cells, purify their proteins, and cut them up. (technologyreview.com)
This mixture is put into the mass spectrometry machine, which sequences the proteins. (technologyreview.com)
Mass spectrometry might be used during cancer drug development to assay a compound's effects on cancer cells' signaling proteins. (technologyreview.com)
New methods and mass spectrometry instrumentation for the characterization of posttranslationally modified proteins and peptides will be presented. (selectbiosciences.com)
The utility of mass spectrometry for peptides and proteins in clinical chemistry will be discussed, using amyloid peptides, tau protein and apolipoprotein E as examples. (selectbiosciences.com)
These advances are made possible by preserving proteins in their native state in solution, followed by careful transfer into the gas phase of the mass spectrometer. (europeanpharmaceuticalreview.com)
Once inside the mass spectrometer, individual protein-ligand complexes can be isolated and dissociated to provide ligand identification or to quantify the stabilisation that ligands provide upon binding to the proteins. (europeanpharmaceuticalreview.com)
A schematic depicting how membrane proteins are studied using native mass spectrometer. (europeanpharmaceuticalreview.com)
Thermo Scientific Orbitrap mass spectrometers in combination with enhanced sensitivity and throughput by multiplexing cells with TMT isobaric labelling allows their platforms to deliver more proteins per cell and from tens to hundreds of cells per day, going beyond one cell at a time. (cision.com)
Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) offers superior mass resolving power and mass measurement accuracy, which is beneficial for the study of intact proteins and the characterisation of their posttranslational modifications (PTMs). (bl.uk)
Mass spectrometry -based shotgun glycomics (MS-SG) is a rapid, sensitive, label-, and immobilization -free approach for the discovery of natural ligands of glycan - binding proteins (GBPs). (bvsalud.org)
Nine proteins were identified by spectrometry. (who.int)

To identify the analytes, experiments were carried out to couple CE with a mass spectrometer. (open.ac.uk)

Instead, he suggests that the real value of using mass spectrometry to analyze a cell's protein content lies in the possibility for a dynamic understanding of how cancer cells behave in different stages of the disease. (technologyreview.com)
One of his former students, a CDC employee, reached out to him to let him know that the branch she worked in was looking for a mass spectrometrist to analyze environmental and biological samples. (cdc.gov)

A Mettler vacuum recording thermoanalyzer interfaced with a Finnigan Model 1050 quadrupole mass spectrometer is described and several applications using the system are given. (cdc.gov)

Forest White , assistant professor of biological engineering at MIT, who uses mass spectrometry to study cell-signaling networks, is more cautious about direct clinical applications for the mass spectrometry technology: "It's hard to imagine a mass spec in every hospital," he says, because the results require expert interpretation. (technologyreview.com)
White points out that even scientists with advanced biological research labs have trouble reading mass spectrometry results. (technologyreview.com)
Discover the Centre for Biological Applications of Mass Spectrometry on the Canada Foundation for Innovation's Research Facilities Navigator. (concordia.ca)

Thermospray techniques used in conjunction with mass spectrometry (MS) with or without high performance liquid chromatographic separation have proven useful for the determination of thermally labile compounds such as toxicant metabolites (Korfmacher et al. (cdc.gov)

He was the head of the Mass Spectrometry Centre at Boehringer Ingelheim Pharmaceuticals in Argentina. (ulster.ac.uk)
This led to the first publication that confirmed 11-βHSD1 as a novel therapeutic target for Alzheimer's disease which has revolutionised the field and became the European centre of excellence in steroid imaging by Mass Spectrometry. (ulster.ac.uk)
Finally, he was appointed Director (SO) of Mass Spectrometry Centre at Ulster (BMRSI), where he is currently in charge of centre facilities and research support. (ulster.ac.uk)

The Infinity series of laboratory gas generators are compressor-less nitrogen systems which have been engineered to provide gas to labs which already have an external source of air, and again is suitable for most mass spectrometers available on the market. (peakscientific.com)
In this webinar, "The Use of MALDI-TOF in the Clinical Laboratory: Effectiveness and Challenges," Dr. Elizabeth Palavecino shares her laboratory's clinical experience with MALDI-TOF mass spectrometry. (beckmancoulter.com)

MALDI-TOF mass spectrometry has emerged as a rapid, cost-efficient and clinically effective tool for identifying pathogens. (beckmancoulter.com)

They know that to perform single cell analysis at scale our mass spectrometers in combination with TMT multiplexing is the way to go. (cision.com)

The researchers were led by Brian Druker of the Oregon Health and Science University in Portland and Roberto Polakiewicz, chief scientific officer of Cell Signaling Technology in Danvers, MA. (technologyreview.com)

Looking at a cell's actual molecular activity using mass spectrometry lets Druker avoid much of the guesswork in searching for cancer mechanisms. (technologyreview.com)
He is a member of British Mass Spectrometry Society (BMSS), Royal Society of Chemistry (RSC), Endocrine Society, Society of Endocrinology, European Society of Molecular Imaging (Chair and editor) and member of advisory board committee of the Irish Mass Spectrometry Society. (ulster.ac.uk)
The primary goal of the work described in this thesis was to employ FT-ICR mass spectrometry to investigate the molecular details of p53 redox-regulation. (bl.uk)

Oregon researchers have used a new mass spectrometry technique to uncover a mutation that can cause leukemia. (technologyreview.com)
Researchers can now pinpoint when early cultures began consuming animal milk and what kind of homemade glue was used to repair a punch bowl owned by George Washington's mother using new mass spectrometry chemical analysis techniques. (aaas.org)

Thermal desorption combined with GC-MS allows us to look at complex mixture and obtain confident identifications with the use of mass spectral libraries. (ru.nl)
Thermal Analysis and Mass Spectrometry. (cdc.gov)

The new technique, called protein mass spectrometry, could uncover other drug targets and reveal how drugs affect cancer cells. (technologyreview.com)
Protein mass spectrometry, in contrast, provides a clearer picture of what the cell is doing. (technologyreview.com)
Protein mass spectrometry profiles of cells from a tumor biopsy could identify which protein is running amok and what drug would work best on it. (technologyreview.com)
Hundreds of candidate protein biomarkers can be rapidly triaged, using MRM mass spectrometry, without the use of antibodies. (selectbiosciences.com)
Mass spectrometry (MS) performed under native-like conditions (nMS) is a promising approach for studying protein-drug interactions, and has already informed treatments of some of the most intractable diseases including cancer, diabetes and Alzheimer's. (europeanpharmaceuticalreview.com)
This allows the mass of protein and their bound ligands to be determined with high accuracy and resolution, elucidating not only their binding stoichiometry but also the capability to observe each species present in solution. (europeanpharmaceuticalreview.com)

Two techniques of mass spectrometry were used within this thesis, fast atom bombardment and electrospray but only electrospray ionisation mass spectrometry was used to couple to capillary electrophoresis and was the only mass spectrometric technique used to analyse clinical and environmental samples. (open.ac.uk)

Become familiar with principles of mass spectrometric techniques and their applications particularly in biomedical research applications. (ulster.ac.uk)
CBAMS takes a leading role in providing Concordia students with hands-on training in the applications of mass spectrometry. (concordia.ca)

Mass/charge ratio measurements were obtained for each analyte used and these allowed the unambiguous identification of each analyte. (open.ac.uk)

An enrichment strategy was developed to purify oxidised p53 and top-down FT-ICR mass spectrometry revealed unambiguously that Cys176, 182, 238 and 242 were the oxidised residues. (bl.uk)

Utilising this additional dimension of separation, it is possible to differentiate highly complex mixtures composed of species of equivalent mass, known as isobaric species, based on different conformations. (europeanpharmaceuticalreview.com)

This involved complexing the diol with a boron-containing acid to produce an anion which could then be detected using ES and FAB mass spectrometric methods. (open.ac.uk)

However, even though training facilities are available and mass spec companies have worked hard to make the 'software more straightforward', many still opt for bypassing the learning altogether and instead send their samples to a research facility that analyses the sample and send back the results. (peakscientific.com)

In most cases the gas that feeds their mass spec is often the least of the scientists' worries - that is until they run out of gas mid analysis. (peakscientific.com)
Is mass spectrometry analysis performed on oligos before shipped? (idtdna.com)
Mass spectral records were processed by principal component analysis (PCA) and stepwise linear discriminant analysis (LDA). (unboundmedicine.com)

Many faculty in the sciences at Concordia use mass spectrometry in their research. (concordia.ca)
RAPID COMMUNICATIONS IN MASS SPECTROMETRY publishes preliminary accounts of recent research in mass spectrometry. (periodicals.com)

Mass spectrometry is what Victor was trained for during his graduate work, so, not one to overlook an opportunity, he sent in his CV. (cdc.gov)

multiple vacuum stages separate by differential apertures (holes), the ions created in the argon plasma are, with the aid of various electrostatic focusing techniques, transmitted through the mass analyzer to the detector(s) and counted. (wikipedia.org)
In the article Perkel discusses the best way for novices to approach mass spectrometry experiments such as attending courses at facilities that offer open-access to instruments and step by step training to master key techniques. (peakscientific.com)

CE instruments were successfully coupled to an electrospray mass spectrometer which then became the detector. (open.ac.uk)

Mass Spectrometry-Based Shotgun Glycomics Using Labeled Glycan Libraries. (bvsalud.org)

Druker's mass spectrometry study focused on acute myeloid leukemia, the most common form of the disease. (technologyreview.com)

However, though mass spectrometry is highly accurate and fast, taking only minutes to convert a specimen into a result, it is not fully. (darkdaily.com)
At the core of nMS is the ability to preserve non-covalent interactions and perform very accurate mass measurements. (europeanpharmaceuticalreview.com)

mass spectrometry is often seen as a complicated and highly technical technique that requires years of experience to master. (peakscientific.com)

Dr. Elizabeth Palavecino is professor of pathology, director of clinical microbiology and co-director of the clinical and translational mass spectrometry center at Wake Forest School of Medicine. (beckmancoulter.com)
2 Center for Structural Mass Spectrometry , Kurt-Mothes-Str. (biorxiv.org)

Proteomic [mass spectrometry] gives you the true readout of what's going on in the cell," says Mark Cobbold, a clinician scientist at the University of Birmingham, U.K. (technologyreview.com)

If you're looking for a mass spectrometrist who can develop a method for your particular project, I am your guy. (cdc.gov)

Dr Diego Cobice has been working/contributing in the field of Mass Spectrometry (MS) from more than 20 years. (ulster.ac.uk)

Other work involved using CE, ES and FAI3 mass spectrometry, to develop a new technique to detect diol containing compounds. (open.ac.uk)

After 7 years in the pharmaceutical sector, he has moved to the University of Edinburgh (Medical School) to pursue a MSc in applied MS to cardiovascular diseases/risk factors using a novel MS modality called "Mass Spectrometry Imaging" (MSI). (ulster.ac.uk)

Anatomy12

Organisms6

Diseases1

Chemicals and Drugs87

Analytical, Diagnostic and Therapeutic Techniques and Equipment83

Phenomena and Processes50

Disciplines and Occupations9

Anthropology, Education, Sociology and Social Phenomena3

Technology, Industry, Agriculture4

Information Science8

Health Care13

Less common "doping" agents and substances encountered during routine screening for drugs. (1/17751)

Urinary lithium: distribution shape, reference values, and evaluation of exposure by inductively coupled plasma argon-emission spectrometry. (2/17751)

Archive of mass spectral data files on recordable CD-ROMs and creation and maintenance of a searchable computerized database. (3/17751)

UV irradiation of polycyclic aromatic hydrocarbons in ices: production of alcohols, quinones, and ethers. (4/17751)

Relationship between UDP-glucose 4-epimerase activity and oligoglucose glycoforms in two strains of Neisseria meningitidis. (5/17751)

Insulin-like growth factors I and II are unable to form and maintain their native disulfides under in vivo redox conditions. (6/17751)

Purification and identification of a novel subunit of protein serine/threonine phosphatase 4. (7/17751)

Identification of 17-methyl-18-norandrosta-5,13(17-dien-3beta-ol, the C19 fragment formed by adrenal side chain cleavage of a 20-aryl analog of (20S)-20-hydroxycholesterol. (8/17751)

Mass Spectrometry

Tandem Mass Spectrometry

Spectrometry, Mass, Electrospray Ionization

Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Gas Chromatography-Mass Spectrometry

Chromatography, Liquid

Chromatography, High Pressure Liquid

Proteomics

Spectrometry, Mass, Secondary Ion

Proteome

Molecular Sequence Data

Amino Acid Sequence

Spectrometry, Mass, Fast Atom Bombardment

Electrophoresis, Gel, Two-Dimensional

Deuterium Exchange Measurement

Peptides

Isotope Labeling

Molecular Structure

Peptide Mapping

Reproducibility of Results

Reference Standards

Calibration

Magnetic Resonance Spectroscopy

Solid Phase Extraction

Deuterium

Carbohydrate Sequence

Sensitivity and Specificity

Limit of Detection

Proteins

Ions

Body Mass Index

Metabolomics

Peptide Fragments

Substance Abuse Detection

Chromatography, Thin Layer

Chromatography, Gas

Fourier Analysis

Cyclotrons

Indicators and Reagents

Protein Processing, Post-Translational

Isotopes

Analytic Sample Preparation Methods

Protein Binding

Trypsin

Metabolome

Electrophoresis, Polyacrylamide Gel

Polysaccharides

Glycosylation

Oligosaccharides

Carbohydrate Conformation

Molecular Weight

Sequence Analysis, Protein

Isomerism

Chromatography, Reverse-Phase

Oxidation-Reduction

Indicator Dilution Techniques

Cattle

Electrophoresis, Capillary

Models, Molecular

Complex Mixtures

Biotransformation

Oxygen Isotopes

Gases

Recombinant Proteins

Chemical Fractionation

Biological Markers

Spectrophotometry, Atomic

Bacterial Proteins

Hydrolysis

Microchemistry

Protein Array Analysis

Forensic Medicine

Hydrogen

Atmospheric Pressure

Time Factors

Nanotechnology

Algorithms

Spectrophotometry, Ultraviolet

Binding Sites

Kinetics