Glycoside hydrolases that catalyze the hydrolysis of alpha or beta linked MANNOSE.
An enzyme that catalyzes the HYDROLYSIS of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. The enzyme plays a role in the processing of newly formed N-glycans and in degradation of mature GLYCOPROTEINS. There are multiple isoforms of alpha-mannosidase, each having its own specific cellular location and pH optimum. Defects in the lysosomal form of the enzyme results in a buildup of mannoside intermediate metabolites and the disease ALPHA-MANNOSIDOSIS.
An alpha-glucosidase inhibitor with antiviral action. Derivatives of deoxynojirimycin may have anti-HIV activity.
An indolizidine alkaloid from the plant Swainsona canescens that is a potent alpha-mannosidase inhibitor. Swainsonine also exhibits antimetastatic, antiproliferative, and immunomodulatory activity.
A hexose or fermentable monosaccharide and isomer of glucose from manna, the ash Fraxinus ornus and related plants. (From Grant & Hackh's Chemical Dictionary, 5th ed & Random House Unabridged Dictionary, 2d ed)
Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.
A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Yeast-like ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES isolated from exuded tree sap.
A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed)
The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
An inborn error of metabolism marked by a defect in the lysosomal isoform of ALPHA-MANNOSIDASE activity that results in lysosomal accumulation of mannose-rich intermediate metabolites. Virtually all patients have psychomotor retardation, facial coarsening, and some degree of dysostosis multiplex. It is thought to be an autosomal recessive disorder.
A mitosporic Trichocomaceae fungal genus that develops fruiting organs resembling a broom. When identified, teleomorphs include EUPENICILLIUM and TALAROMYCES. Several species (but especially PENICILLIUM CHRYSOGENUM) are sources of the antibiotic penicillin.
Antibiotic and mycotoxin from Aspergillus niveus and Penicillium citrinum.
A plant genus in the CHENOPODIACEAE family.
Compounds that differ from COUMARINS in having the positions of the ring and ketone oxygens reversed so the keto oxygen is at the 1-position of the molecule.
A hemeprotein from leukocytes. Deficiency of this enzyme leads to a hereditary disorder coupled with disseminated moniliasis. It catalyzes the conversion of a donor and peroxide to an oxidized donor and water. EC 1.11.1.7.
Tissue that supports and binds other tissues. It consists of CONNECTIVE TISSUE CELLS embedded in a large amount of EXTRACELLULAR MATRIX.
Any disturbances of the normal rhythmic beating of the heart or MYOCARDIAL CONTRACTION. Cardiac arrhythmias can be classified by the abnormalities in HEART RATE, disorders of electrical impulse generation, or impulse conduction.
An intracellular proteinase found in a variety of tissue. It has specificity similar to but narrower than that of pepsin A. The enzyme is involved in catabolism of cartilage and connective tissue. EC 3.4.23.5. (Formerly EC 3.4.4.23).
An electrical current applied to the HEART to terminate a disturbance of its rhythm, ARRHYTHMIAS, CARDIAC. (Stedman, 25th ed)
A potentially lethal cardiac arrhythmia that is characterized by uncoordinated extremely rapid firing of electrical impulses (400-600/min) in HEART VENTRICLES. Such asynchronous ventricular quivering or fibrillation prevents any effective cardiac output and results in unconsciousness (SYNCOPE). It is one of the major electrocardiographic patterns seen with CARDIAC ARREST.
The N-acetyl derivative of glucosamine.
A nucleoside diphosphate sugar which can be converted to the deoxy sugar GDPfucose, which provides fucose for lipopolysaccharides of bacterial cell walls. Also acts as mannose donor for glycolipid synthesis.
Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.
Eicosamethyl octacontanonadecasen-1-o1. Polyprenol found in animal tissues that contains about 20 isoprene residues, the one carrying the alcohol group being saturated.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.
Social media model for enabling public involvement and recruitment in participation. Use of social media to collect feedback and recruit volunteer subjects.
The terms, expressions, designations, or symbols used in a particular science, discipline, or specialized subject area.
A research and development program initiated by the NATIONAL LIBRARY OF MEDICINE to build knowledge sources for the purpose of aiding the development of systems that help health professionals retrieve and integrate biomedical information. The knowledge sources can be used to link disparate information systems to overcome retrieval problems caused by differences in terminology and the scattering of relevant information across many databases. The three knowledge sources are the Metathesaurus, the Semantic Network, and the Specialist Lexicon.
A specified list of terms with a fixed and unalterable meaning, and from which a selection is made when CATALOGING; ABSTRACTING AND INDEXING; or searching BOOKS; JOURNALS AS TOPIC; and other documents. The control is intended to avoid the scattering of related subjects under different headings (SUBJECT HEADINGS). The list may be altered or extended only by the publisher or issuing agency. (From Harrod's Librarians' Glossary, 7th ed, p163)

Oligosaccharide modification in the early secretory pathway directs the selection of a misfolded glycoprotein for degradation by the proteasome. (1/742)

The role of conformation-based quality control in the early secretory pathway is to eliminate misfolded polypeptides and unassembled multimeric protein complexes from the endoplasmic reticulum, ensuring the deployment of only functional molecules to distal sites. The intracellular fate of terminally misfolded human alpha1-antitrypsin was examined in hepatoma cells to identify the functional role of asparagine-linked oligosaccharide modification in the selection of glycoproteins for degradation by the cytosolic proteasome. Proteasomal degradation required physical interaction with the molecular chaperone calnexin. Altered sedimentation of intracellular complexes following treatment with the specific proteasome inhibitor lactacystin, and in combination with mannosidase inhibition, revealed that the removal of mannose from attached oligosaccharides abrogates the release of misfolded alpha1-antitrypsin from calnexin prior to proteasomal degradation. Intracellular turnover was arrested with kifunensine, implicating the participation of endoplasmic reticulum mannosidase I in the disposal process. Accelerated degradation occurred in a mannosidase-independent manner and was arrested by lactacystin, in response to the posttranslational inhibition of glucosidase II, demonstrating that the attenuated removal of glucose from attached oligosaccharides functions as the underlying rate-limiting step in the proteasome-mediated pathway. A model is proposed in which the removal of mannose from multiple attached oligosaccharides directs calnexin in the selection of misfolded alpha1-antitrypsin for degradation by the proteasome.  (+info)

Molecular cloning, sequencing, and expression of a novel multidomain mannanase gene from Thermoanaerobacterium polysaccharolyticum. (2/742)

The manA gene of Thermoanaerobacterium polysaccharolyticum was cloned in Escherichia coli. The open reading frame of manA is composed of 3,291 bases and codes for a preprotein of 1,097 amino acids with an estimated molecular mass of 119,627 Da. The start codon is preceded by a strong putative ribosome binding site (TAAGGCGGTG) and a putative -35 (TTCGC) and -10 (TAAAAT) promoter sequence. The ManA of T. polysaccharolyticum is a modular protein. Sequence comparison and biochemical analyses demonstrate the presence of an N-terminal leader peptide, and three other domains in the following order: a putative mannanase-cellulase catalytic domain, cellulose binding domains 1 (CBD1) and CBD2, and a surface-layer-like protein region (SLH-1, SLH-2, and SLH-3). The CBD domains show no sequence homology to any cellulose binding domain yet reported, hence suggesting a novel CBD. The duplicated CBDs, which lack a disulfide bridge, exhibit 69% identity, and their deletion resulted in both failure to bind to cellulose and an apparent loss of carboxymethyl cellulase and mannanase activities. At the C-terminal region of the gene are three repeats of 59, 67, and 56 amino acids which are homologous to conserved sequences found in the S-layer-associated regions within the xylanases and cellulases of thermophilic members of the Bacillus-Clostridium cluster. The ManA of T. polysaccharolyticum, besides being an extremely active enzyme, is the only mannanase gene cloned which shows this domain structure.  (+info)

Endometrial lysosomal enzyme activity in normal cycling endometrium. (3/742)

The objective of this study was to evaluate the possible role of four lysosomal enzymes in endometrial function and remodelling during the normal menstrual cycle by fluorimetric measurement (acid phosphatase, N-acetyl-beta-D-glucosaminidase, alpha-L-fucosidase and alpha-D-mannosidase). A prospective study was conducted of 45 endometrial biopsies obtained from women with normal menstrual cycles. Activity of all four enzymes was identified in human endometrium. Activity of acid phosphatase and N-acetyl-beta-D-glucosaminidase was relatively high, whilst that of alpha-L-fucosidase and alpha-D-mannosidase was low. There was no significant change in the activity of any of the four enzymes from the proliferative to the secretory phase of the cycle. This study suggests that the activity of these enzymes remains constant throughout a major portion of the normal cycle.  (+info)

Cloning and sequencing of beta-mannosidase gene from Aspergillus aculeatus no. F-50. (4/742)

The manB gene, coding for a unique beta-mannosidase (MANB) of Aspergillus aculeatus, was cloned from genomic and cDNA libraries, and sequenced. The gene consists of 2,811 bp encoding a polypeptide of 937 amino acid residues with a molecular mass of 104,214 Da. The A. aculeatus MANB shared amino acid sequence identity with MANB of human (24%), goat (24%), bovine (24%), and Caenorhabditis elegans (22%). When the A. aculeatus MANB was compared with other related enzymes, a Glu residue corresponding to the active site identified by the Escherichia coli beta-galactosidase and the human beta-guclonidase was conserved. This is the first fungal gene that encodes MANB.  (+info)

Uptake of iodinated human kidney alpha-D-mannosidase by rat liver- Association with membrane elements and stability in vivo and in vitro. (5/742)

1. Human kidney alpha-D-mannosidase (form A) was labelled with 125I to a specific radio-activity of approx. 2250muCi/mg of protein, essentially without loss of enzymic activity. The enzymic activity and radioactivity of the iodinated material also co-migrated in gel filtration and gel electrophoresis. 2. The binding of 125I-labelled mannosidase in vitro to particulate material in liver and kidney homogenates was of the other of 2 pg/mg of particulate material in liver and kidney homogenates was of the order of 2pg/mg of particulate protein withing 16h at 37 degrees C, and essentially zero in intervals of up to 60 min. The degradation in vitro of labelled exogenous mannosidase was of the order of 10-20pg/ 16th per mg of protein in postnuclear supernatant, and it was saturated entirely within 1h at 37 degrees C. 3. The binding of labelled mannosidase in vivo to particulate elements of liver homogenates 60 min after intravenous injection was at least 10 times higher in terms of specific radioactivity than the highest value attainable in vitro. Virtually all exogenous enzyme bound to liver particulate material could be recovered in macromolecular form after disruption of membranes by detergents. 4. The radioactive enzyme bound to liver particulate material could be detached almost completely by shearing, repeated freezing and thawing, and exposure to strong detergents under conditions that do not eliminate rough-endoplasmic-membrane structure. It could bot be released, however, by high salt concentration (0.5M-KC1) or by exposure to weak detergents such as Tween 80. The particle-bound enzyme should thus be associated with plasma membranes and lysosome-like elements. 5. Of the rat tissues studied, only liver could approach, within 60 min after the injection, the concentration of exogenous mannosidase found in the blood serum. The activity per g tissue weight fell progressively from liver (60% of serum value) to kidney (16% of serum value), lung (8% of serum vlaue), spleen (6% of serum value) and brain (0.9% of serum value). Most of the radioactive enzyme found in tissues other than liver appeared to be present in a free form, whereas in liver more than 50% of the labelled enzyme was associated with membrane elements.  (+info)

Inhibition of secretion by 1,3-Cyclohexanebis(methylamine), a dibasic compound that interferes with coatomer function. (6/742)

We noted previously that certain aminoglycoside antibiotics inhibit the binding of coatomer to Golgi membranes in vitro. The inhibition is mediated in part by two primary amino groups present at the 1 and 3 positions of the 2-deoxystreptamine moiety of the antibiotics. These two amines appear to mimic the epsilon-amino groups present in the two lysine residues of the KKXX motif that is known to bind coatomer. Here we report the effects of 1, 3-cyclohexanebis(methylamine) (CBM) on secretion in vivo, a compound chosen for study because it contains primary amino groups that resemble those in 2-deoxystreptamine and it should penetrate lipid bilayers more readily than antibiotics. CBM inhibited coatomer binding to Golgi membranes in vitro and in vivo and inhibited secretion by intact cells. Despite depressed binding of coatomer in vivo, the Golgi complex retained its characteristic perinuclear location in the presence of CBM and did not fuse with the endoplasmic reticulum (ER). Transport from the ER to the Golgi was also not blocked by CBM. These data suggest that a full complement of coat protein I (COPI) on membranes is not critical for maintenance of Golgi integrity or for traffic from the ER to the Golgi but is necessary for transport through the Golgi to the plasma membrane.  (+info)

Isolation and purification of a neutral alpha(1,2)-mannosidase from Trypanosoma cruzi. (7/742)

Trypanosoma cruzi is an obligatory intracellular protozoan parasite that causes Chagas' disease in humans. Although a fair amount is known about the biochemistry of certain trypanosomes, very little is known about the enzymic complement of synthesis and processing of glycoproteins and/or functions of the subcellular organelles in this parasite. There have been very few reports on the presence of acid and neutral hydrolases in Trypanosoma cruzi. Here we report the first purification and characterization of a neutral mannosidase from the epimastigote stage of Trypanosoma cruzi. The neutral mannosidase was purified nearly 800-fold with an 8% recovery to apparent homogeneity from a CHAPS extract of epimastigotes by the following procedures: (1) metal affinity chromatography on Co+2-Sepharose, (2) anion exchange, and (3) hydroxylapatite. The purified enzyme has a native molecular weight of 150-160 kDa and is apparently composed of two subunits of 76 kDa. The purified enzyme exhibits a broad pH profile with a maximum at pH 5.9-6.3. It is inhibited by swainsonine (Ki, 0.1 microM), D-mannono-delta-lactam (Ki, 20 microM), kifunensine (Ki, 60 microM) but not significantly by deoxymannojirimycin. The enzyme is activated by Co2+and Ni2+and strongly inhibited by EDTA and Fe2+. The purified enzyme is active against p-nitrophenyl alpha-D-mannoside (km = 87 microM). High-mannose Man9GlcNAc substrate was hydrolyzed by the purified enzyme to Man7GlcNAc at pH 6.1. The purified enzyme does not show activity against alpha1,3- or alpha1,6-linked mannose residues. Antibodies against the recently purified lysosomal alpha-mannosidase from T.cruzi did not react with the neutral mannosidase reported here.  (+info)

Molecular and enzymic properties of recombinant 1, 2-alpha-mannosidase from Aspergillus saitoi overexpressed in Aspergillus oryzae cells. (8/742)

For the construction of an overexpression system of the intracellular 1,2-alpha-mannosidase (EC 3.2.1.113) gene (msdS) from Aspergillus saitoi (now designated Aspergillus phoenicis), the N-terminal signal sequence of the gene was replaced with that of the aspergillopepsin I (EC 3.4.23.18) gene (apnS) signal, one of the same strains as described previously. Then the fused 1, 2-alpha-mannosidase gene (f-msdS) was inserted into the NotI site between P-No8142 and T-agdA in the plasmid pNAN 8142 (9.5 kbp) and thus the Aspergillus oryzae expression plasmid pNAN-AM1 (11.2 kbp) was constructed. The fused f-msdS gene has been overexpressed in a transformant A. oryzae niaD AM1 cell. The recombinant enzyme expressed in A. oryzae cells was purified to homogeneity in two steps. The system is capable of making as much as about 320 mg of the enzyme/litre of culture. The recombinant enzyme has activity with methyl-2-O-alpha-d-mannopyranosyl alpha-D-mannopyranoside at pH 5.0, while no activity was determined with methyl-3-O-alpha-D-mannopyranosyl alpha-D-mannopyranoside or methyl-6-O-alpha-D-mannopyranosyl alpha-D-mannopyranoside. The substrate specificity of the enzyme was analysed by using pyridylaminated (PA)-oligomannose-type sugar chains, Man9-6(GlcNAc)2-PA (Man is mannose; GlcNAc is N-acetylglucosamine). The enzyme hydrolysed Man8GlcNAc2-PA (type 'M8A') fastest, and 'M6C' {Manalpha1-3[Manalpha1-2Manalpha1-3(Manalpha1-6) Manalpha1-6]Manbeta1- 4GlcNAcbeta1-4GlcNAc-PA} slowest, among the PA-sugar chains. Molecular-mass values of the enzyme were determined to be 63 kDa by SDS/PAGE and 65 kDa by gel filtration on Superose 12 respectively. The pI value of the enzyme was 4.6. The N-terminal amino acid sequence of the enzyme was GSTQSRADAIKAAFSHAWDGYLQY, and sequence analysis indicated that the signal peptide from apnS gene was removed. The molar absorption coefficient, epsilon, at 280 nm was determined as 91539 M-1.cm-1. Contents of the secondary structure (alpha-helix, beta-structure and the remainder of the enzyme) by far-UV CD determination were about 55, 38 and 7% respectively. The melting temperature, Tm, of the enzyme was 71 degrees C by differential scanning calorimetry. The calorimetric enthalpy, DeltaHcal, of the enzyme was calculated as 13.3 kJ.kg of protein-1. Determination of 1 g-atom of Ca2+/mol of enzyme was performed by atomic-absorption spectrophotometry.  (+info)

9.0 9.1 9.2 Gonzalez, DS et al. (1999) Identification, expression, and characterization of a cDNA encoding human endoplasmic reticulum mannosidase I, the enzyme that catalyzes the first mannose trimming step in mammalian Asn-linked oligosaccharide biosynthesis. J. Biol. Chem. 274 21375-86 PubMed GONUTS page ...
Cytosolic α-mannosidases are glycosyl hydrolases that participate in the catabolism of cytosolic free N-oligosaccharides. Two soluble α-mannosidases (E-I and E-II) belonging to glycosyl hydrolases family 47 have been described in Candida albicans. We demonstrate that addition of pepstatin A during the preparation of cell homogenates enriched α-mannosidase E-I at the expense of E-II, indicating that the latter is generated by proteolysis during cell disruption. E-I corresponded to a polypeptide of 52 kDa that was associated with mannosidase activity and was recognized by an anti-α1,2-mannosidase antibody. The N-mannan core trimming properties of the purified enzyme E-I were consistent with its classification as a family 47 α1,2-mannosidase. Differential density-gradient centrifugation of homogenates revealed that α1,2-mannosidase E-I was localized to the cytosolic fraction and Golgi-derived vesicles, and that a 65 kDa membrane-bound α1,2-mannosidase was present in endoplasmic reticulum and Golgi
Shop a large selection of products and learn more about ERManI Mouse anti-Human, Mouse, Alexa Fluor 594, Clone: 3C2, Novus Biologicals ERManI Antibody; Alexa Fluor™ 594;
We use cookies to ensure that we give you the best experience on our website. If you click Continue well assume that you are happy to receive all cookies and you wont see this message again. Click Find out more for information on how to change your cookie settings ...
β-mannosidase (EC 3.2.1.25, MANB) dissects the non-reducing end of N-linked mannose moieties of glycoproteins in eukaryotic cells. The human β-mannosidase gene was amplified by RT-PCR, cloned and sequenced. The DNA sequence was compared with reported human β-mannosidase DNA sequence and sixteen nucleotide differences were found. The deduced amino-acid sequence showed that seven codons coded the same amino acids and nine codons coded different amino acids with reference to nucleotide substitution positions but did not affect recombinant MANB enzyme activity. No splice mutation was observed after comparison with reported MANB DNA sequences. A 75% homology of deduced amino-acid sequence was observed with mouse, goat and bovine β-mannosidase amino-acid sequences. The cloned β-mannosidase gene was subcloned into pET22b+ and pET28a+ expression vectors to transform the BL21-codon plus cells for expression of recombinant MAN22 and MAN28 enzymes, respectively. The optimized conditions for ...
In all cells derived from animals, plants, fungi and protozoa a single protein called UGGT acts as a quality control checkpoint that inspects secreted glycoproteins. UGGT has fascinated scientists for many years as it has the capacity to check the quality of a huge array of glycoproteins, which come in many different shapes and sizes and folds. The way in which it screens such a diverse group of proteins is poorly understood, and no structures exist of UGGT. Furthermore, UGGT is implicated in human diseases as it can ensure that glycoproteins from viruses (such as influenza, hepatitis C, HIV and Zika) are folded correctly. By fully characterising UGGT, it is hoped that an inhibitor could be developed that may act as a broad-spectrum anti-viral drug. Using the Macromolecular Crystallography (MX) beamlines (I02, I03, I04 and I04-1) and cryo-electron microscopy (cryo-EM) at the Electron Bio-Imaging Centre (eBIC), the structure of UGGT was solved. Four distinct crystal structures of a full-length UGGT
Mannonojiritetrazole (7), a novel mannosidase inhibitor, has been synthesized in six steps from 2,3,4,6-tetra-O-benzyl-D-mannose oxime. The structure of 7 has been established by X-ray analysis. The solid state conformation of 7 is H-6(7) (=H-4(3), numbering based on carbohydrate nomenclature), and the conformation in CD3OD is close to S-7 (sofa; = S-3, numbering based upon carbohydrate nomenclature), while the conformation of the previously synthesized analogue with the gluco configuration (6) is H-6(7), both in the solid state and in solution in D2O or CD3OD. Both 6 and 7 have been tested as inhibitors of each of a series of five alpha- and beta-glucosidases and -mannosidases as well as of a beta-galactosidase, and inhibition constants have been determined. A good correlation (p = 0.9) was found between log K-i for each inhibitor-enzyme pair and log (V-m/K-m) for the corresponding substrate-enzyme pair, thereby providing the first such proof for any glycosidase inhibitor being a transition ...
Seeds of Triticale (hybrid of wheat and rye) contain an N-acetylglucosamine specific lectin that was affinity purified in our laboratory (Siva Kumar, N. and Padma, K. (1996) Affinity purification of N-acetyl glucosamine specific lectin. Purification and partial characterization of Triticale lectin. Biochem. Mol. Biol. Int. 38, 1059-1066). Seed extracts also exhibited alpha-mannosidase activity that was isolated by a combination of ion exchange, hydrophobic chromatography and gel filtration. The purified enzyme is a glycoprotein with 7% carbohydrate and exhibited a native molecular mass of 1,95,000 (+/-5000) on Biogel P-200 and dissociated into two major subunits under reducing conditions of molecular masses 58 and 40 kDa, respectively. Both subunits cross-reacted with an antibody to the well-characterized jack bean alpha-mannosidase, suggesting antigenic similarity between the legume and the cereal mannosidases. Purified enzyme binds to Con A-Sepharose gel, possibly through the sugar-binding site.
While multiple alpha 1-2-mannosidases are necessary for glycoprotein N-glycan maturation in vertebrates, a single bacterial alpha 1-2-mannosidase can be sufficient to cleave all alpha 1-2-linked mannose residues in host glycoprotein N-glycans. We report here the characterization and crystal structure of a new alpha 1-2-mannosidase (EfMan-I) from Enterococcus faecalis, a Gram-positive opportunistic human pathogen. EfMan-I catalyzes the cleavage of alpha 1-2-mannose from not only oligomannoses but also high-mannose-type N-glycans on glycoproteins. Its 2.15 angstrom resolution crystal structure reveals a two-domain enzyme fold similar to other CAZy GH92 mannosidases. An unexpected potassium ion was observed bridging two domains near the active site. These findings support EfMan-I as an effective catalyst for in vitro N-glycan modification of glycoproteins with high-mannose-type N-glycans ...
alpha-Mannosidase (EC 3.2.1.24, alpha-D-mannosidase, p-nitrophenyl-alpha-mannosidase, alpha-D-mannopyranosidase, 1,2-alpha-mannosidase, 1,2-alpha-D-mannosidase, exo-alpha-mannosidase) is an enzyme involved in the cleavage of the alpha form of mannose. Its systematic name is alpha-D-mannoside mannohydrolase.[3][4] ...
TY - JOUR. T1 - Molecular cloning and primary structure of Man9‐mannosidase from human kidney. AU - BAUSE, Ernst. AU - BIEBERICH, Erhard. AU - ROLFS, Andreas. AU - VÖLKER, Christof. AU - SCHMIDT, Bernhard. PY - 1993/10. Y1 - 1993/10. N2 - Man9‐mannosidase, a processing enzyme found in the endoplasmic reticulum (ER), catalyses the removal of three distinct mannose residues from peptide‐bound Man9‐GlcNAc2 oligosaccharides producing a single Man6 isomer [Bause, E., Breuer, W., Schweden, J., Roesser, R. & Geyer, R. (1992) Eur. J. Biochem. 208, 451-457]. We have isolated four Man9‐mannosidase‐specific clones from a human kidney cDNA library and used these to construct a full‐length cDNA of 3250 base pairs. A single open reading frame of 1875 nucleotides encodes a protein of approximately 71 kDa, consistent with data from immunological studies. Analysis of the coding sequence predicts that Man9‐mannosidase is a type II transmembrane protein consisting of a short cytoplasmic ...
A recent study in Genetics in Medicine aiming at the identification by whole-exome sequencing of genes involved in infantile nystagmus found heterozygous missense mutations in the MANBA gene, which the encodes lysosomal beta-mannosidase. The mutations resulted in decrease of ?-mannosidase activities in the patients as well as in mutant-transfected HEK293T cells. MANBA is expressed in the pretectal nucleus of the developing midbrain, […]. ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
In eukaryotes, class I alpha-mannosidases are involved in early N-glycan processing reactions and in N-glycan-dependent quality control in the endoplasmic reticulum (ER). To investigate the role of these enzymes in plants, we identified the ER-type alpha-mannosidase I (MNS3) and the two Golgi-alpha-mannosidase I proteins (MNS1 and MNS2) from Arabidopsis thaliana. All three MNS proteins were found to localize in punctate mobile structures reminiscent of Golgi bodies. Recombinant forms of the MNS proteins were able to process oligomannosidic N-glycans. While MNS3 efficiently cleaved off one selected alpha 1,2-mannose residue from Man(9)GlcNAc(2), MNS1/2 readily removed three alpha 1,2-mannose residues from Man(8)GlcNAc(2). Mutation in the MNS genes resulted in the formation of aberrant N-glycans in the mns3 single mutant and Man(8)GlcNAc(2) accumulation in the mns1 mns2 double mutant. N-glycan analysis in the mns triple mutant revealed the almost exclusive presence of Man(9)GlcNAc(2), ...
Diseases caused by the loss of one or more enzymes involved in the hydrolysis of mannoside linkages (MANNOSIDASES). The defects in enzyme activity are primarily associated with genetic mutation of the genes that codes for a particular mannosidase isoenzyme ...
a Crude β-mannanase recovery from solid medium. b Crude β-mannanase recovery from liquid medium. Data shown here are average of quadruplicate measurements wit
1PS3: Comparison of Kifunensine and 1-Deoxymannojirimycin Binding to Class I and II alpha-Mannosidases Demonstrates Different Saccharide Distortions in Inverting and Retaining Catalytic Mechanisms
In the case of Pdi1p, the correlation between accessibility to ERManI and the degree of processing is striking. The modeling also predicted that a domain deletion would expose a glycosylation site on a neighboring domain to processing, which was confirmed experimentally. For influenza HA, we were able to rationalize why certain sites remained as Man9GN2. Further, the modeling work was able to propose a 3D model for how the pulmonary collectin SP-D would bind these Man9GN2 glycans and thus neutralize the virus. When applied to the HIV envelope protein, our modeling approach provides insight into formation of the so-called high-mannose patch.. Conclusion: ...
Complete information for EDEM2 gene (Protein Coding), ER Degradation Enhancing Alpha-Mannosidase Like Protein 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Alpha Mannosidosis is a progressive disorder, and its presence should be suspected in patients with cognitive disabilities, skeletal changes (e.g. swollen joints, curved spine), hearing loss and recurrent infections. Although children with the condition are often born seemingly normal, their condition deteriorates with age. Alpha-mannosidosis can impact on a patients quality of life in many ways, including their ability to live independently, socialise or find employment.[2][7] Generally, phenotypes of alpha-mannosidosis patients are not clearly distinguishable, which makes a prediction of the clinical course for an individual patient challenging.[2] Patients may present to doctors, nurses or health visitors at different stages of progression, and with different ad hoc symptoms, making the link to suspect a diagnosis of alpha-mannosidosis difficult.[2] The main symptoms can also be shared with those of other lysosomal storage disorders, such as mucopolysaccharidosis.[2] Given the progressive ...
The alpha-Mannosidosis Mutation Database compiles all alpha-Mannosidosis mutations that have been reported in the published literature. This information is useful to compile patterns and to draw hypotheses on disease etiology. It also allows the analysis of genotype/phenotype relationships.
Misfolded proteins are recognized in the endoplasmic reticulum (ER), transported back to the cytoplasm and degraded by the proteasome. Processing intermediates of N-linked oligosaccharides on incompletely folded glycoproteins have an important role in their folding/refolding, and also in their targeting to proteolytic degradation. In Saccharomyces cerevisiae, we have identified a gene coding for a non-essential protein that is homologous to mannosidase I (HTM1) and that is required for degradation of glycoproteins. Deletion of the HTM1 gene does not affect oligosaccharide trimming. However, deletion of HTM1 does reduce the rate of degradation of the mutant glycoproteins such as carboxypeptidase Y, ABC-transporter Pdr5-26p and oligosaccharyltransferase subunit Stt3-7p, but not of mutant Sec61-2p, a non-glycoprotein. Our results indicate that although Htm1p is not involved in processing of N-linked oligosaccharides, it is required for their proteolytic degradation. We propose that this mannosidase ...
[39 Pages Report] Check for Discount on Alpha-Mannosidosis Global Clinical Trials Review, H2, 2016 report by GlobalData. GlobalDatas clinical trial report,
Mannosidosis is a genetic disorder characterized by a lysosomal enzyme deficiency resulting in progressive mental and physical deterioration.
여러 종류의 mannanase를 생산하는 Cellulosimicrobium sp. YB-43으로부터 mannanase B를 암호하는 manB 유전자와 효소의 특성이 보고된 바 있다. Mannanase C (ManC)로 명명한 효소의 유전자가 manB 유전자의 하류에 위치한 것으로 예상되어 이를 중합효소 연쇄반응으로 클로닝하여 manC 유전자의 염기서열을 결정하였다. ManC는 448 아미노산 잔기로 구성된 것으로 확인되었으며 glycosyl hydrolase family 5에 속하는 mannanase와 상동성이 높은 활성영역과 탄수화물 결합영역(CBM2)이 존재하였다. ManC의 활성영역은 Streptomyces sp. SirexAA-E (55.8%; 4FK9_A) 및 S. thermoluteus (57.6%; BAM62868)의 mannanase와 아미노산 배열의 상동성이 55% 이상으로 가장 높았다. Signal peptide 영역이 제거되고 카르복실 말단에 hexahistidine이 연결되도록 제조한 His-tagged ManC (HtManC)의 유전자를 재조합 대장균에서 발현하여 균체 ...
The sequence variant c.1010G,A (dbSNP rs1133330) in exon 7 changes argnine in amino acid position 337 with glutamine (p.Arg337Gln). It has a minor allele frequency of 0.26-0.41 in different populations, and is considered a common, benign SNP. Amino acid residue 337 is located on the surface of the MAN2B1 protein, and studies in transfected cells show that p.Gln337 is active and processed correctly. ...
Mannanases - Instruments Consumables Reagents Advanced BioMatrix,RANDOX,RANDOX ELISA,Biomedical, biochemical reagents, laboratory supplies, equipment, antibodies, ELISA kits, diagnostic reagents, methods of experimental techniques, general analytical instruments, material testing instruments and equipment, used laboratory equipment, instruments and equipment, life sciences, environmental monitoring equipment , measurement, measuring instruments, rotating wall bioreactor, three-dimensional tissue / stem cell culture system; microcapsule
Purchase high purity enzyme endo-1-4 beta-Mannanase (Bacillus sp.) for for use in research, biochemical enzyme assays and in vitro diagnostic analy...
In the Lindemann chemical kinetic treatment of the isomerization of molecule A to its isomer B, as in the isomerization reaction of cyclopropane to propene
anti-alfa-mannosidase antibodies, plant mannosidase antibodies, AS09 452Alpha-mannosidase cleaves the alpha form of mannose. anti-alfa-mannosidase antibodies
Retro Classic herreur fra det kendte mærke Jacques Lemans. Henrik Ørsnes er autoriseret forhandler af Jacques Lemans ure. Køb nemt og trygt her.
Archibald Leman Cochrane: Archibald Leman Cochrane, British physician who contributed greatly to the development of epidemiology, emphasized the necessity for randomized control trials (RCTs) in
β-mannanase (EC 3.2.1.78); exo-β-1,4-mannobiohydrolase (EC 3.2.1.100); β-1,3-xylanase (EC 3.2.1.32); lichenase / endo-β-1,3-1,4-glucanase (EC 3.2.1.73); mannobiose-producing exo-β-mannanase (EC 3.2.1.- ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Restore your car with this new 1963-68 LeMans Transmission Case Bolts, Muncie 4-Speed Black Oxide Hex Head. The ultimate in performance fasteners. Complete with everything you need. Tighten up your Muncie 4-speed transmission with one of these premium ARP bolt sets. These bolts are rated at a tensile strength of 170,00
With Greys Anatomy hitting its tenth season, it should come as no surprise that Patrick Dempsey may be getting restless. But the actor got a breath of fresh air last year after entering LeMans, Frances 24-hour sports car race.Dempsey, who ...
Cumpara Idei creative 87 - Papusi de mana - Kocsis Eszter pe Libris. Transport gratuit >90 lei si livrare rapida. 30 de zile retur.
MAN2B2 antibody [N1N2], N-term (mannosidase, alpha, class 2B, member 2) for IHC-P, WB. Anti-MAN2B2 pAb (GTX120905) is tested in Human samples. 100% Ab-Assurance.
Ida Leman, Actress: Badai di Ujung Negeri. Ida Leman is an actress, known for Badai di Ujung Negeri (2011), Perempuan berkalung sorban (2009) and Melintas Badai (1985).
Platekompaniet - For å gi deg en bedre opplevelse av nettbutikken bruker Platekompaniet cookies. I innstillingene til din nettleser kan du selv velge å slette informasjonskapsler ...
Perhaps I just forgot how leveling is, and no this isnt about leveling in Wrath being too hard or too slow. I just find my spriest (currently lv73) to have to refrain from using some spells too often otherwise hes constantly struggling with low mana. Thats inspite of Shadowfiend and Dispersion. Thus Im feeling slowed down by that. Whats your take? Is mana an issue for you?
Macam mana nak buat kuah lebih pekat? Selepas Yui masak, yui hanya ambil sedikit sahaja iaitu cukup untuk satu hidangan. Ketika menggelegak, terus masukkan bancuhan tepung jagung ( sikit saja ) dan terus kacau. Autotamatik kuah akan jadi pekat dan likat seperti gambar bawah ...
I shift my weight and put my toes on the base of the dialysis machine, right next to the bicarbonate jugs. A great way to stretch my calf; good to do several times during an ICU run. I only hold it a few seconds before switching to the second needle site. Luckily this patient is lucid and strong enough to hold pressure on his arm, so I get a jump start on wiping down all the equipment and telling his nurse on the floor how he did. Twenty minutes later they wheel him away and I tell him Hasta luego, even though its a lie. After locking up the needles and paperwork Im leaving here and not coming back. Im not a dialysis nurse anymore ...
α-Mannosidosis (MIM 248500) is an autosomal recessive lysosomal storage disorder resulting from deficient activity of lysosomal α-mannosidase (LAMAN) (EC 3.2.1.24). The disease is characterized by massive intracellular accumulation of mannose-rich oligosaccharides with resulting mental retardation, hearing loss, immune deficiency and skeletal changes. We report here the purification and characterization of human placenta LAMAN. The enzyme is synthesized as a single-chain precursor which is processed into three glycopeptides of 70, 42 and 15 kDa. The 70 kDa peptide is further partially proteolysed into three more peptides that are joined by disulfide bridges. The laman cDNA sequence was assembled from overlapping fragments obtained by PCR on human fibroblast and human lung cDNA. The deduced amino acid sequence contains a putative signal peptide of 48 amino acids followed by a polypeptide sequence of 962 amino acids. Northern blot analyses revealed a single transcript of ∼3.5 kb present in all ...
We investigated the influence of a glycosylphosphatidylinositol (GPI) anchor on the ectodomain of the influenza hemagglutinin (HA) by replacing the wild type (wt) transmembrane and cytoplasmic domains with a GPI lipid anchor. GPI-anchored HA (GPI-HA) was transported to the cell surface with equal efficiency and at the same rate as wt-HA. Like wt-HA, cell surface GPI-HA, and its ectodomain released with the enzyme PI-phospholipase C (PI-PLC), were 9S trimers. Compared to wt-HA, the GPI-HA ectodomain underwent additional terminal oligosaccharide modifications; some of these occurred near the receptor binding pocket and completely inhibited the ability of GPI-HA to bind erythrocytes. Growth of GPI-HA-expressing cells in the presence of the mannosidase I inhibitor deoxymannojirimycin (dMM) abrogated the differences in carbohydrate modification and restored the ability of GPI-HA to bind erythrocytes. The ectodomain of GPI-HA produced from cells grown in the presence or absence of dMM underwent ...
Mannan endo-1,4-beta-mannosidase is an enzyme with system name 4-beta-D-mannan mannanohydrolase. This enzyme catalyses the following chemical reaction: Random hydrolysis
SWISS-MODEL Repository entry for A1C8U0 (MANF_ASPCL), Mannan endo-1,4-beta-mannosidase F. Aspergillus clavatus (strain ATCC 1007 / CBS 51365 / DSM 816 / NCTC 3887 /NRRL 1)
Do lipids such as sphingomyelin (SM) that are known to assemble into specific membrane domains play a role in the organization and function of transmembrane proteins? With this paper we display that disruption of SM homeostasis in the trans-Golgi network (TGN) by treatment of HeLa cells with d-ceramide-C6 which was converted together with phosphatidylcholine to short-chain SM and diacylglycerol by SM synthase led to the segregation of Golgi-resident proteins from each other. Our results suggest that SM organizes transmembrane proteins into practical enzymatic domains in the TGN. Intro Newly synthesized proteins are core glycosylated in the ER GRS after which the sugars chains are trimmed and revised in the Golgi complex. This process takes place inside a spatially and timely regulated manner as trimming of the core glycosylations by mannosidases in the cis- and medial-Golgi cisternae is definitely a requirement for complex glycosylation in later on Golgi compartments (Stanley 2011 What is the ...
1DL2: Crystal structure of a class I alpha1,2-mannosidase involved in N-glycan processing and endoplasmic reticulum quality control.
|p||b|High dose, 5 mg methylated folate,|/b||b||i| as|/i||/b||b| the all natural 6S isomer|/b||br| |br| |b|L-5-MTHF|/b| is a safe and effective way to increase the bodys folate levels. It is especially helpful for patients with demonstrated increased n
FREE SHIPPING! Classic Lemans Styling. Give your Mustang the classic styling of yesteryear with a set of Matte Black Lemans Style Stripes. These AmericanMuscle
Comprar Laxar Purgante Natural El Mana Online. Venta del laxante natural Laxar de El Mana, mejor purgante ✅ Tienda Naturista Bogotá ✅ Envío Rápido
Apabila kita dah lalui sendiri taklah ngeri mana, sakit tu memang sakit tapi sakit itu hilang apabila kita dapat tengok zuriat sendiri depan mata lagi-lagi ini anak sulung, orang kata confirm kena gunting, lepas tu kau kena jahit, nak buang air besar pun susah nanti tapi alhamdulillah atas izin Allah, nak melahirkan Adam Malique ni tiada luka terkoyak dan kena jahit. Baby keluar 2.8 kilogram dan ramai juga tanya macam mana boleh tiada koyak, hebat. Saya tak hebat cuma rezeki saya bersalin tiada kena jahit, katanya menerusi akaun Facebook miliknya, malam tadi. ...
DIAMANTS DU LEMAN - Designer French doors from PanoramAH! ✓ all information ✓ high-resolution images ✓ CADs ✓ catalogues ✓ contact information..
@horvath heres my vintage pox list in its last iteration. I played it a few times. I did pretty well with it once. If you catch the right xerox decks you can just go under them and destroy their mana base. Its a lot of fun regardless. Very light on win...
My max mana: 11115 I cast flash heal: 2300 I get 87 mana back. Edit: If Im suppose to get back 2.5% on a flash heal, that means I should be getting back 277. Which I clearly am not. Does anyone know if this is a bug... or is what Rapture means: You can only get back a total of 2.5% mana on a big heal... So far from Im reading into - this new Rapture totally sucks (like I said when it first came out) Lets hope Im wrong.
Wanted to share my report from the NYSE with a normally unplayed archetype that Ive put a lot of work into lately. I played UB Ad Nauseam and ended 5-2. My breakers were terrible so I ended up in 20th place despite several 15 pointers making top 16. The ...
... beta-mannosidase (EC 3.2.1.25); myrosinase (EC 3.2.1.147). 6-phospho-beta-galactosidase InterPro: IPR005928 GBA3; KL; KLB; LCT ...
... CAZY GH_47 comprises enzymes with only one known activity; alpha-mannosidase (EC 3.2.1.113). ... Alpha-mannosidase is involved in the maturation of Asn-linked oligosaccharides. The enzyme hydrolyses terminal 1,2-linked alpha ... 2-mannosidase involved in the processing of asparagine-linked oligosaccharides". The Journal of Biological Chemistry. 269 (13 ...
... beta-mannosidase (EC 3.2.1.25); beta-glucuronidase (EC 3.2.1.31). These enzymes contain a conserved glutamic acid residue which ...
Inhibitors of B-Mannosidases?". Helv. Chim. Acta. 85 1118-. Cope, A.C.; Mehta, A.S. (1963). "Mechanism of the Hofmann ...
March 2001). "Mannosidase production by viridans group streptococci". J. Clin. Microbiol. 39 (3): 995-1001. doi:10.1128/JCM. ...
"Entrez Gene: MANEA mannosidase, endo-alpha". Land A, Braakman I (2001). "Folding of the human immunodeficiency virus type 1 ... Glycoprotein endo-alpha-1,2-mannosidase is an enzyme that in humans is encoded by the MANEA gene. GRCh38: Ensembl release 89: ... Spiro MJ, Bhoyroo VD, Spiro RG (1997). "Molecular cloning and expression of rat liver endo-alpha-mannosidase, an N-linked ...
4-beta-mannosidases. Mannan endo-1,4-beta-mannosidase hydrolyses mannan and galactomannan, but displays little activity towards ...
Stegelmeier BL, Ralphs MH, Gardner DR, Molyneux RJ, James LF (October 1994). "Serum alpha-mannosidase activity and the ... As the estimated intake of swainsonine increased, blood serum alpha-mannosidase activity and albumin decreased, and alkaline ... Swainsonine inhibits a lysosomal enzyme, alpha-mannosidase. This results in abnormal accumulation of the molecules normally ... and analyzing serum for alpha-mannosidase activity and swainsonine. In mule deer, clinical signs of locoism are similar to ...
"OMIM Entry - * 609489 - MANNOSIDASE, BETA A, LYSOSOMAL; MANBA". www.omim.org. Retrieved 9 May 2018. Johnson, William (2015). ... Beta-mannosidosis, also called lysosomal beta-mannosidase deficiency, is a disorder of oligosaccharide metabolism caused by ... Beta-mannosidase function is consistent with, it being a lysosomal enzyme catalyzing and thus involved in degradation route for ... Beta-mannosidase Alpha-mannosidosis "Mannosidosis, beta A, lysosomal , Genetic and Rare Diseases Information Center (GARD) - an ...
... glucosidases and mannosidases". J. Am. Chem. Soc. 65: 1369-1380. doi:10.1021/ja01247a029. Inulinase at the US National Library ...
The domain is predominantly found in the enzyme alpha-mannosidase. Henrissat B, Callebaut I, Fabrega S, Lehn P, Mornon JP, ... Glycoside hydrolase family 38 CAZY GH_38 comprises enzymes with only one known activity; alpha-mannosidase (EC 3.2.1.24) (EC ... "The structure of bovine lysosomal alpha-mannosidase suggests a novel mechanism for low-pH activation". Journal of Molecular ... 3.2.1.114). Lysosomal alpha-mannosidase is necessary for the catabolism of N-linked carbohydrates released during glycoprotein ...
... is a deficiency in mannosidase, an enzyme. There are two types: Alpha-mannosidosis Beta-mannosidosis Swainsonine ...
6-mannosidase EC 3.2.1.-, α-mannosidase EC 3.2.1.24, α-1,2-mannosidase EC 3.2.1.-, α-1,3-mannosidase EC 3.2.1.- and α-1,4- ... This domain occurs within alpha-1,2-mannosidases, which remove alpha-1,2-linked mannose residues from Man(9)(GlcNAc)(2) by ... Glycoside hydrolase family 92 includes enzymes with mannosyl-oligosaccharide α-1,2-mannosidase EC 3.2.1.113, mannosyl- ... mannosidase EC 3.2.1.- activities. It includes enzymes critical for the maturation of N-linked oligosaccharides and ER- ...
Epididymis-specific alpha-mannosidase precursor Mannosidase alpha class 2B member core-specific lysosomal alpha-1,6-Mannosidase ... "Entrez Gene: MAN2B2 mannosidase, alpha, class 2B, member 2". Land A, Braakman I (2001). "Folding of the human immunodeficiency ... Epididymis-specific alpha-mannosidase is an enzyme that in humans is encoded by the MAN2B2 gene. ... 1998). "Stage-specific expression of a mouse homologue of the porcine 135kDa alpha-D-mannosidase (MAN2B2) in type A ...
"Entrez Gene: MAN1B1 mannosidase, alpha, class 1B, member 1". Land A, Braakman I (2001). "Folding of the human immunodeficiency ... Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase is an enzyme that in humans is encoded by the MAN1B1 gene ... Tremblay LO, Herscovics A (Nov 1999). "Cloning and expression of a specific human alpha 1,2-mannosidase that trims Man9GlcNAc2 ... 2-mannosidase gene involved in N-glycan maturation". Glycobiology. 8 (6): 585-95. doi:10.1093/glycob/8.6.585. PMID 9592125. v t ...
1997). "Man9-mannosidase from pig liver is a type-II membrane protein that resides in the endoplasmic reticulum. cDNA cloning ... "Entrez Gene: MAN1A1 mannosidase, alpha, class 1A, member 1". Land A, Braakman I (2001). "Folding of the human immunodeficiency ... Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA is an enzyme that in humans is encoded by the MAN1A1 gene. This gene encodes ... Bieberich E, Bause E (1995). "Man9-mannosidase from human kidney is expressed in COS cells as a Golgi-resident type II ...
ER mannosidase catalyses the removal of this final glucose. However, if the protein is not folded properly, the glucose ... In the cis-Golgi, a series of mannosidases remove some or all of the four mannose residues in α-1,2 linkages. Whereas in the ...
"Molecular cloning and expression of cDNAs encoding human alpha-mannosidase II and a previously unrecognized alpha-mannosidase ... Alpha-mannosidase 2 is an enzyme that in humans is encoded by the MAN2A1 gene. This gene encodes a protein which is a member of ... "Entrez Gene: MAN2A1 mannosidase, alpha, class 2A, member 1". Land A, Braakman I (Aug 2001). "Folding of the human ... Moremen KW, Touster O (Jun 1985). "Biosynthesis and modification of Golgi mannosidase II in HeLa and 3T3 cells". The Journal of ...
"Entrez Gene: MAN1A2 mannosidase, alpha, class 1A, member 2". Land A, Braakman I (2001). "Folding of the human immunodeficiency ... Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB is an enzyme that in humans is encoded by the MAN1A2 gene. GRCh38: Ensembl ... 2-mannosidase gene involved in N-glycan maturation". Glycobiology. 8 (6): 585-595. doi:10.1093/glycob/8.6.585. PMID 9592125. " ...
alpha-Mannosidase at the US National Library of Medicine Medical Subject Headings (MeSH) Medicine portal v t e. ... Velmanase alfa is a recombinant form of human alpha-mannosidase. Velmanase alfa was approved for medical use in the European ...
ER degradation-enhancing alpha-mannosidase-like 1 is an enzyme that in humans is encoded by the EDEM1 gene. GRCh38: Ensembl ... "Entrez Gene: EDEM1 ER degradation enhancer, mannosidase alpha-like 1". Nagase T, Seki N, Ishikawa K, et al. (1997). "Prediction ... 2001). "A novel ER alpha-mannosidase-like protein accelerates ER-associated degradation". EMBO Rep. 2 (5): 415-22. doi:10.1093/ ...
... inhibits human endoplasmic reticulum α-1,2-mannosidase I and Golgi Class I mannosidases IA, IB and IC with Ki ... Kifunensine shows no inhibitory action against mannosidase II or the endoplasmic reticulum alpha-mannosidase, and it weakly ... Kifunensine does not inhibit mannosidase II or the endoplasmic reticulum alpha-mannosidase. It weakly inhibits arylmannosidase ... Kifunensine is a potent inhibitor of the mannosidase I enzyme. It is 50 to 100 times more potent than deoxymannojirimycin - an ...
ER degradation-enhancing alpha-mannosidase-like 3 is an enzyme that in humans is encoded by the EDEM3 gene. GRCh38: Ensembl ... "Entrez Gene: EDEM3 ER degradation enhancer, mannosidase alpha-like 3". Maruyama K, Sugano S (1994). "Oligo-capping: a simple ...
ER degradation-enhancing alpha-mannosidase-like 2 is an enzyme that in humans is encoded by the EDEM2 gene. GRCh38: Ensembl ... "Entrez Gene: EDEM2 ER degradation enhancer, mannosidase alpha-like 2". Andersson B, Wentland MA, Ricafrente JY, et al. (1996 ...
"A novel ER α‐mannosidase‐like protein accelerates ER‐associated degradation." EMBO Reports 2.5 (2001): 415-422. Moremen, Kelley ...
Jacob CA, Bodmer D, Spirig U, Battig P, Marcil A, Dignard D, Bergeron JJ, Thomas DY, Aebi M (2001). "Htm1p, a mannosidase-like ... Hosokawa N, Wada I, Hasegawa K, Yorihuzi T, Tremblay LO, Herscovics A, Nagata K (2001). "A novel ER alpha-mannosidase-like ... Yos-9 recognizes mannose residues exposed after α-mannosidase removal of an outer mannose of misfolded glycoproteins. Calnexin ...
The inhibitory effect of swainsonine on Golgi Mannosidase II (GMII) was proposed to be due to its ability to bind in the GMII ... It is a potent inhibitor of Golgi alpha-mannosidase II, an immunomodulator, and a potential chemotherapy drug. As a toxin in ... The actual state in which swainsonine binds in the mannosidase remains undetermined and is most likely dependent on the pH at ... Disruption of Golgi alpha-mannosidase II with swainsonine induces hybrid-type glycans. These glycans have a Man5GlcNAc2 core ...
Mannosidase I creates a Man5GlcNAc2-structure on the protein, but note that this has a different structure than the one made on ... Next, a GlcNAc residue forms GlcNAc1Man5GlcNAc2, the substrate for a-mannosidase II (aManII). aManII then removes two Man ...
Using a guar-gum based medium, β-mannanase, β-mannosidase, α-galactosidase have been purified. These galactomannans are ... McCutchen, Carol M. (1997). "Purification and Characterization of Extremely Thermostable B-Mannanase, B-Mannosidase, and A- ...
"Alpha-mannosidase-II deficiency results in dyserythropoiesis and unveils an alternate pathway in oligosaccharide biosynthesis ...
Mannan endo-1,6-alpha-mannosidase (EC 3.2.1.101, exo-1,6-beta-mannanase, endo-alpha-1-,6-D-mannanase, endo-1,6-beta-mannanase, ... Mannan endo-1,6-alpha-mannosidase at the US National Library of Medicine Medical Subject Headings (MeSH) ... mannan endo-1,6-beta-mannosidase, 1,6-alpha-D-mannan mannanohydrolase) is an enzyme with systematic name 6-alpha-D-mannan ... Retrieved from "https://en.wikipedia.org/w/index.php?title=Mannan_endo-1,6-alpha-mannosidase&oldid=777533804" ...
Mannosidase is an enzyme which hydrolyses mannose. There are two types: alpha-Mannosidase beta-Mannosidase A deficiency is ... Mannosidases at the US National Library of Medicine Medical Subject Headings (MeSH) Biology portal v t e. ... A family of mannosidases are also responsible for processing newly formed glycoproteins in the endoplasmic reticulum into ...
... alpha-D-mannosidase, p-nitrophenyl-alpha-mannosidase, alpha-D-mannopyranosidase, 1,2-alpha-mannosidase, 1,2-alpha-D-mannosidase ... Humans express the following three alpha-mannosidase isozymes: It can be utilized in experiments that determine the effects of ... Liberation of D-mannose from various glycoproteins by α-mannosidase isolated from jack bean meal". J. Biol. Chem. 241 (4): 1010 ... GeneReviews/NCBI/NIH/UW entry on Alpha-Mannosidosis OMIM entries on Alpha-Mannosidosis alpha-Mannosidase at the US National ...
1-2,3 Mannosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of ?1-2 and ?1-3 linked mannose residues ... α1-2,3 Mannosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of α1-2 and α1-3 linked mannose residues ... α1-2,3 Mannosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of α1-2 and α1-3 linked D-Mannopyranosyl ... α1-2,3 Mannosidase. P0729SVIAL. 4 1 x 0.02 ml 32,000 units/ml ... What is a good positive control for this α1-2, 3 Mannosidase? ...
alpha-1,2-Mannosidase. Primary source. WormBase:WBGene00008411 Locus tag. CELE_D2030.1 Gene type. protein coding. RNA name ... alpha-1,2-Mannosidase. RefSeq status. REVIEWED. Organism. Caenorhabditis elegans (strain: Bristol N2) Lineage. Eukaryota; ... mannosyl-oligosaccharide 1,2-alpha-mannosidase activity IBA Inferred from Biological aspect of Ancestor. more info ... NM_059715.5 → NP_492116.1 alpha-1,2-Mannosidase [Caenorhabditis elegans]. See identical proteins and their annotated locations ...
GO:0016985 mannan endo-1,4-beta-mannosidase activity GO:0030246 carbohydrate binding ...
Hultberg, B.: Properties of α-mannosidase in mannosidosis. Scand. J. Clin. Lab. Invest. 26: 155, 1970.PubMedCrossRefGoogle ... Phillips, N.C., Robinson, D. and Winchester, B.G.: Human liver α-mannosidase activity. Clin. Chim. Acta 55: 11, 1974.PubMed ... Snaith, S.M.: Characterization of jack-bean α-D-mannosidase as a zinc metalloenzyme. Biochem. J. 147: 83, 1975.PubMedGoogle ... Snaith, S.M., Hay, A.J. and Levvy, G.A.: Relation between the α-mannosidase activity and the zinc content of mammalian sex ...
lysosomal alpha-mannosidase. Names. lysosomal acid alpha-mannosidase. mannosidase alpha-B. mannosidase, alpha B, lysosomal. NP_ ... MAN2B1 mannosidase alpha class 2B member 1 [Homo sapiens] MAN2B1 mannosidase alpha class 2B member 1 [Homo sapiens]. Gene ID: ... mannosidase alpha class 2B member 1provided by HGNC. Primary source. HGNC:HGNC:6826 See related. Ensembl:ENSG00000104774 MIM: ... PLN02701; alpha-mannosidase. cd10810. Location:63 → 341. GH38N_AMII_LAM_like; N-terminal catalytic domain of lysosomal alpha- ...
"Molecular cloning and expression of cDNAs encoding human alpha-mannosidase II and a previously unrecognized alpha-mannosidase ... "Molecular cloning and expression of cDNAs encoding human alpha-mannosidase II and a previously unrecognized alpha-mannosidase ... "Molecular cloning and expression of cDNAs encoding human alpha-mannosidase II and a previously unrecognized alpha-mannosidase ... Alpha-mannosidase 2x (Mannosidase, alpha, class 2A, member 2, isoform CRA_f) ...
Beta-mannosidaseAdd BLAST. 862. Amino acid modifications. Feature key. Position(s). DescriptionActions. Graphical view. Length ... "Caprine beta-mannosidase: sequencing and characterization of the cDNA and identification of the molecular defect of caprine ... sp,Q95327,MANBA_CAPHI Beta-mannosidase OS=Capra hircus OX=9925 GN=MANBA PE=2 SV=1 ...
BETA-MANNOSIDASE. A, B. 846. Bacteroides thetaiotaomicron VPI-5482. Mutation(s): 0 Gene Names: BT_0458. EC: 3.2.1.25. ... Structural and Biochemical Evidence for a Boat-Like Transition State in Beta-Mannosidases.. Tailford, L.E., Offen, W.A., Smith ... Structural and biochemical evidence for a boat-like transition state in beta-mannosidases. *DOI: 10.2210/pdb2VMF/pdb ... Here we present the analysis of 25 putative beta-mannosidase inhibitors, whose Ki values range from nanomolar to millimolar, on ...
... alpha-D-mannosidase, p-nitrophenyl-alpha-mannosidase, alpha-D-mannopyranosidase, 1,2-alpha-mannosidase, 1,2-alpha-D-mannosidase ... File:Tonge redo alpha mannosidase.png Cartoon depiction of the protein Streptococcus pyogenes family GH38 α-Mannosidase created ... Liberation of D-mannose from various glycoproteins by α-mannosidase isolated from jack bean meal". J. Biol. Chem. 241 (4): 1010 ... Winchester, B. (1984). "Role of α-D-mannosidases in the biosynthesis and catabolism of glycoproteins". Biochem. Soc. Trans. 12 ...
2 Mannosidase IA antibody. Validated in WB, ICC/IF and tested in Mouse, Rat, Human. Cited in 1 publication(s). Independently ... Anti-alpha 1,2 Mannosidase IA antibody. See all alpha 1,2 Mannosidase IA primary antibodies. ... Anti-alpha 1,2 Mannosidase IA antibody (ab51262) at 1/1000 dilution + Human colon whole cell lysate at 40 µg. Secondary. HRP- ... Western blot - Anti-alpha 1,2 Mannosidase IA antibody (ab51262)This image is courtesy of an Abreview submitted by Praveen ...
View mouse Man1b1 Chr2:25332730-25352213 with: sequences, polymorphisms, proteins, references, function, expression
What is beta-mannosidase deficiency? Meaning of beta-mannosidase deficiency medical term. What does beta-mannosidase deficiency ... Looking for online definition of beta-mannosidase deficiency in the Medical Dictionary? beta-mannosidase deficiency explanation ... Beta-mannosidase activity in plasma, fibroblasts and leukocytes is minimal.. Molecular pathology. Defects in MANBA, which ... Beta-mannosidase deficiency , definition of beta-mannosidase deficiency by Medical dictionary https://medical-dictionary. ...
View and select products within our alpha-mannosidase Inhibitors category available for immediate purchase. ... ChemCruz™ Chemical alpha-mannosidase Substrates for functional analysis of cellular responses to alpha-mannosidase ... A selective inhibitor of α-mannosidases 73465-43-7. sc-255823 sc-255823A 1 mg. 5 mg. $80.00 $135.00 2 ... A mannose analog that acts as an inhibitor of a-mannosidase 108032-93-5. sc-222158 1 g. $220.00 0 ...
beta-mannosidase , mannosidase, beta A, lysosomal , beta-mannosidase-like , hypothetical protein , Beta-mannosidase , lysosomal ... Mannosidase, beta A, Lysosomal (MANBA) ELISA Kit ELISA Kit MANBA Reaktivität: Maus Colorimetric Sandwich ELISA 6.25-400 ng/mL ... Mannosidase, beta A, Lysosomal (MANBA) Antigen-Profil Protein Überblick This gene encodes a member of the glycosyl hydrolase 2 ... Mannosidase, beta A, Lysosomal (MANBA) ELISA Kit ELISA Kit MANBA Reaktivität: Human Colorimetric Competition ELISA 0.78-50 ng/ ...
An inborn error of metabolism marked by a defect in the lysosomal isoform of BETA-MANNOSIDASE that results in lysosomal ... beta-Mannosidase Deficiency; Deficiency, beta-Mannosidase; beta Mannosidase Deficiency; beta Mannosidosis; beta-Mannosidase ... MannosidasesIBA 01/01/1992 - "We describe the biochemical aspects of these beta-mannosidosis and the properties of the beta- ... mannosidases of mammalian origin. ". 06/01/1982 - "This investigation indicates that deficiency of plasma beta-mannosidases ...
Man9-mannosidase , alpha-1,2-mannosidase IA , man(9)-alpha-mannosidase , mannosyl-oligosaccharide 1,2-alpha-mannosidase IA , ... processing alpha-1,2-mannosidase IA , mannosidase 1, alpha , mannosyl-oligosaccharide alpha-1,2-mannosidase , mannosidase alpha ... anti-Mannosidase, Alpha, Class 1A, Member 1 Antikörper MAN1A1 encodes a class I mammalian Golgi 1,2-mannosidase which is a type ... Target Mannosidase, Alpha, Class 1A, Member 1 (MAN1A1) * Mannosidase, Alpha, Class 1A, Member 1 (MAN1A1) ...
α-Mannosidase from Canavalia ensiformis (Jack bean), ammonium sulfate suspension, ≥15 units/mg protein (biuret) pricing ... Rapid screening for Chlamydia trachomatis infection by detecting α-mannosidase activity in urogenital tract specimens.. [Ze-yu ... We established another new rapid non-culture method for detection of C. trachomatis based on the measurement of α-mannosidase ... Only C. trachomatis was positive for α-mannosidase activity among different types of microbes tested in the research. When ...
2-mannosidase reveals the basis for differences in specificity of the endoplasmic reticulum and Golgi class I enzymes. ... Class I alpha-1;2-mannosidase, catalytic domain Class I alpha-1;2-mannosidase, catalytic domain Fungus (Penicillium citrinum) [ ... Class I alpha-1;2-mannosidase, catalytic domain Class I alpha-1;2-mannosidase, catalytic domain Fungus (Penicillium citrinum) [ ... Mannosyl-oligosaccharide alpha-1,2-mannosidase (1KRF:A,B) * Mannosyl Oligosaccharide 1 2 Alpha Mannosidase Activity ...
2-mannosidase, catalytic domain family. Additional information, provided for both this family and the superfamily it belongs to ... Mannosyl-oligosaccharide 1,2-alpha-mannosidase. 0. 2.929. --. DIRECT. Enzyme Commission (EC). Hydrolases. 0. 0.4043. Least ... Class I alpha-1;2-mannosidase, catalytic domain family. SCOP classification Root: SCOP hierarchy in SUPERFAMILY [. 0] (11) ... Mannosyl-oligosaccharide 1,2-alpha-mannosidase. 0. 2.741. --. DIRECT. Enzyme Commission (EC). Glycosidases, i.e. enzymes ...
HomeAlpha-mannosidase-II deficiency results in dyserythr. Alpha-mannosidase-II deficiency results in dyserythr. By admin ...
Oligomannosides or oligosaccharide-lipids as potential substrates for rat liver cytosolic α-d-mannosidase. Thierry GRARD, ... We have previously reported the substrate specificity of the cytosolic α-D-mannosidase purified from rat liver using Man9GlcNAc ... These results suggest a role for α-D-mannosidase in the catabolism of glycans released from the endoplasmic reticulum rather ... We study here the activity of the purified cytosolic α-D-mannosidase towards the oligosaccharide-diphosphodolichol ...
What is ER degradation-enhancing -mannosidase-like protein 3? Meaning of ER degradation-enhancing -mannosidase-like protein 3 ... What does ER degradation-enhancing -mannosidase-like protein 3 mean? ... mannosidase-like protein 3 in the Medical Dictionary? ER degradation-enhancing -mannosidase-like protein 3 explanation free. ... ER degradation-enhancing -mannosidase-like protein 3 , definition of ER degradation-enhancing -mannosidase-like protein 3 by ...
Inhibition of Helix pomatia beta-mannosidase using p-nitrophenyl glycoside as substrate assessed as residual activity at 1 mM ...
... identity with the equivalent mannosidases from Sacchromyces cerevesiae. ER mannosidase from G. antartica was then cloned into ... Cloning and expression of N-glycosylation-related mannosidase from Glaciozyma antarctica for the production of a mannosynthase ... In this study, endoplasmic reticulum 1,2-α-mannosidase from Glaciozyma antractica was recombinantly expressed in Pichia ... The gene sequence for ER mannosidase was obtained from the Glaciozyma antractica database. The BLAST (Basic Local Alignment ...
We have examined the expression of one of these genes, alpha-mannosidase. Using an alpha-mannosidase cDNA probe in Northern ( ... However, cell density did play a major role in the expression of alpha-mannosidase. High cell density or the presence of buffer ... Finally, the alpha-mannosidase gene was not expressed in aggregation-negative mutant strain HMW 404. ... Developmental regulation of the alpha-mannosidase gene in Dictyostelium discoideum: control is at the level of transcription ...
Purchase high purity recombinant enzyme beta-Mannosidase (Cellulomonas Fimi) for use in research, biochemical enzyme assays and ... One Unit of β-D-mannosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per ... β-Mannosidases were present in both endosperm and cotyledon-embryo and could be separated chromatographically. The level of ... High purity recombinant β-Mannosidase (Cellilomonas fimi) for use in research, biochemical enzyme assays and in vitro ...
The plasma alpha-mannosidase assay is a rapid and reliable screening test for the mannosidosis genotype and for detecting ... The plasma alpha-mannosidase assay is a rapid and reliable screening test for the mannosidosis genotype and for detecting ... The plasma alpha-mannosidase assay is a rapid and reliable screening test for the mannosidosis genotype and for detecting ... The plasma alpha-mannosidase assay is a rapid and reliable screening test for the mannosidosis genotype and for detecting ...
  • Lysosomal storage disease in the brain: mutations of the β-mannosidase gene identified in autosomal dominant nystagmus. (nih.gov)
  • The gene sequence for ER mannosidase was obtained from the Glaciozyma antractica database. (open.ac.uk)
  • Developmental regulation of the alpha-mannosidase gene in Dictyostelium discoideum: control is at the level of transcription and is affected by cell density. (asm.org)
  • Finally, the alpha-mannosidase gene was not expressed in aggregation-negative mutant strain HMW 404. (asm.org)
  • Mutations in the gene ( MAN2B1 ) encoding lysosomal α-D-mannosidase cause improper coding, resulting in dysfunctional or non-functional protein, causing the disease α-mannosidosis. (biomedcentral.com)
  • A gene encoding mannan endo-1,4-β-mannosidase or 1,4-β- D -mannan mannanohydrolase (E.C. 3.2.1.78), commonly termed β-mannanase, from Aspergillus niger BK01, which belongs to glycosyl hydrolase family 5 (GH5), was cloned and successfully expressed heterologously (up to 243 μg of active recombinant protein per mL) in Pichia pastoris . (biomedcentral.com)
  • Three peptides were generated, two of which matched a tentative consensus sequence in the TIGR C. posadasii 2.0 gene index database that is similar to fungal 1,2-α-mannosidases. (elsevier.com)
  • It is caused by mutations of the MAN2B1 gene, which lead to alpha-mannosidase deficiency. (symptoma.com)
  • In Saccharomyces cerevisiae, we have identified a gene coding for a non-essential protein that is homologous to mannosidase I (HTM1) and that is required for degradation of glycoproteins. (unibas.ch)
  • To identify novel genes contributing to the development of IBD, we analyzed single nucleotide polymorphisms (SNPs) of glycosylation-related genes in IBD patients and identified MAN2A1, encoding alpha-mannosidase II (α-MII), as a candidate gene. (cdc.gov)
  • Alpha-mannosidase I function was altered by i) treatment with Kifunensine, a specific inhibitor for class I alpha-mannosidases, ii) downregulation of alpha-mannosidase I gene expression using siRNA transfection, and iii) overexpression utilising retroviral gene transfer. (fu-berlin.de)
  • MAN2B1 (Mannosidase Alpha Class 2B Member 1) is a Protein Coding gene. (genecards.org)
  • Gene Ontology (GO) annotations related to this gene include carbohydrate binding and alpha-mannosidase activity . (genecards.org)
  • The MANBA gene provides instructions for making the enzyme beta-mannosidase. (medlineplus.gov)
  • Uchino Y, Fukushige T, Yotsumoto S, Hashiguchi T, Taguchi H, Suzuki N, Konohana I, Kanzaki T. Morphological and biochemical studies of human beta-mannosidosis: identification of a novel beta-mannosidase gene mutation. (medlineplus.gov)
  • [2] In humans it is known to be caused by an autosomal recessive genetic mutation in the gene MAN2B1, located on chromosome 19, affecting the production of the enzyme alpha-D-mannosidase, resulting in its deficiency. (wikipedia.org)
  • The C. albicans MNS1 gene is predicted to encode a member of the glycosyl hydrolase family 47, with α 1,2-mannosidase activity. (fiocruz.br)
  • BACKGROUND & AIMS: Human tumors and liver cancer cell lines express the product of a fusion between the first 13 exons in the mannosidase α class 2A member 1 gene (MAN2A1) and the last 6 exons in the FER tyrosine kinase gene (FER), called MAN2A1-FER. (bireme.br)
  • The human β-mannosidase gene was amplified by RT-PCR, cloned and sequenced. (edu.pl)
  • The cloned β-mannosidase gene was subcloned into pET22b+ and pET28a+ expression vectors to transform the BL21-codon plus cells for expression of recombinant MAN22 and MAN28 enzymes, respectively. (edu.pl)
  • Polymorphism in the endoplasmic reticulum mannosidase I (MAN1B1) gene is not associated with liver disease in individuals homozygous for the Z variant of the alpha1-antitrypsin protease inhibitor (PiZZ individuals). (cdc.gov)
  • Traffic of human α-mannosidase in plant cells suggests the presence of a new endoplasmic reticulum-to-vacuole pathway without involving the Golgi complex. (nih.gov)
  • MAN1A1 encodes a class I mammalian Golgi 1,2-mannosidase which is a type II transmembrane protein. (antikoerper-online.de)
  • They also inhibit glycan processing alpha-mannosidases in the Golgi complex and block maturation to complex type glycan structures on cell surface and secreted glycoproteins. (grantome.com)
  • The unique interdisciplinary team that we have assembled takes advantage of ongoing synergistic collaborative interactions between investigators at the University of Georgia and Baylor College of Medicine with expertise in the synthesis of selective glycosidase inhibitors (Boons), the biochemistry and structural biology of the ER and Golgi mannosidases (Moremen), and cell-based assays for a human glycoprotein misfolding disorder, alpha1-antitrypsin deficiency (Sifers). (grantome.com)
  • The amino acid sequence of several peptide regions, including a calcium‐binding consensus sequence, bears striking similarities to an ER α‐1,2‐mannosidase from yeast, whereas, by contrast, no sequence similarity was detectable with rat liver ER α‐mannosidase and Golgi α‐mannosidase II. (elsevier.com)
  • This distribution overlaps that of several Golgi and vesicle markers, including mannosidase II, p58, trans-Golgi network (TGN)38, and β-COP and is distinct from the endoplasmic reticulum markers calnexin and Bip. (pnas.org)
  • In this study, we examined the transglycosylating activity of a Golgi endo-α-mannosidase. (bireme.br)
  • We prepared various glycosyl donors and acceptors, and recombinant human Golgi endo-α-mannosidase and its various mutants were expressed. (bireme.br)
  • Mannosidase 2 is a 135kD protein located on the luminal side of the Golgi membranes. (acris-antibodies.com)
  • Differential density-gradient centrifugation of homogenates revealed that α 1,2-mannosidase E-I was localized to the cytosolic fraction and Golgi-derived vesicles, and that a 65 kDa membrane-bound α 1,2-mannosidase was present in endoplasmic reticulum and Golgi-derived vesicles. (microbiologyresearch.org)
  • Using an alpha-mannosidase cDNA probe in Northern (RNA) blot analysis, we have shown that the previously observed increase in alpha-mannosidase enzyme-specific activity during development is due to an increase in the levels of alpha-mannosidase mRNA. (asm.org)
  • The 5′ and 3′ ends of the mannosidase cDNA were mapped using rapid amplification of cDNA ends (RACE) polymerase chain reaction (PCR), and a full-length cDNA was then obtained using reverse-transcription (RT) PCR. (elsevier.com)
  • We have isolated four Man 9 ‐mannosidase‐specific clones from a human kidney cDNA library and used these to construct a full‐length cDNA of 3250 base pairs. (elsevier.com)
  • Human beta-mannosidase cDNA characterization and first identification of a mutation associated with human beta-mannosidosis. (medlineplus.gov)
  • Alkhayat AH, Kraemer SA, Leipprandt JR, Macek M, Kleijer WJ, Friderici KH (1998) Human β-mannosidase cDNA characterization and first identification of a mutation associated with human β-mannosidosis. (edu.pl)
  • 100 μg) Partial protein comprised of amino acids 1 - 86 of the human mannosidase beta A lysosomal-like (MANBAL) protein. (allelebiotech.com)
  • Mannosidase) Is Required for HIV-1 Envelope Glycoprotein Degradation via Endoplasmic Reticulum-associated Protein Degradation Pathway. (grantome.com)
  • alpha-Mannosidase I N-linked protein glycosylation represents an important cellular process for modifying protein properties. (fu-berlin.de)
  • Analysis of the coding sequence predicts that Man 9 ‐mannosidase is a type II transmembrane protein consisting of a short cytoplasmic polypeptide tail, a single transmembrane domain acting as a non‐cleavable signal sequence and a large luminal catalytic domain. (elsevier.com)
  • The protein sequence of the Man 9 ‐mannosidase contains three potential N‐glycosylation sites of which only one site is used. (elsevier.com)
  • Endoplasmic reticulum degradation-enhancing α-mannosidase-like protein 1 targets misfolded HLA-B27 dimers for endoplasmic reticulum-associated degradation. (uclan.ac.uk)
  • The ER stress-induced protein ER degradation-enhancing α-mannosidase-like protein 1 (EDEM1) was overexpressed by transfection, and dimer levels were monitored by immunoblotting. (uclan.ac.uk)
  • A family of mannosidases are also responsible for processing newly formed glycoproteins in the endoplasmic reticulum into mature glycoproteins containing highly heterogeneous complex-type glycans. (wikipedia.org)
  • We study here the activity of the purified cytosolic α- D -mannosidase towards the oligosaccharide-diphosphodolichol intermediates formed during the biosynthesis of N -glycans, and also towards soluble oligosaccharides released from the endoplasmic reticulum which are glucosylated or not and possessing at their reducing end either a single N -acetylglucosamine residue or a di- N -acetylchitobiose sequence. (biochemj.org)
  • These results suggest a role for α- D -mannosidase in the catabolism of glycans released from the endoplasmic reticulum rather than in the regulation of the biosynthesis of asparagine-linked oligosaccharides. (biochemj.org)
  • In this study, endoplasmic reticulum 1,2-α-mannosidase from Glaciozyma antractica was recombinantly expressed in Pichia pastoris . (open.ac.uk)
  • This enzyme functions in N-glycan biosynthesis, and is a class I alpha-1,2-mannosidase that specifically converts Man9GlcNAc to Man8GlcNAc isomer B. It is required for N-glycan trimming to Man5-6GlcNAc2 in the endoplasmic-reticulum-associated degradation pathway. (nih.gov)
  • These properties are similar to those displayed by Mns1 purified from C . albicans membranes and strongly suggest that the enzyme is an α 1,2-mannosidase that is localised to the endoplasmic reticulum and involved in the processing of N- linked mannans. (fiocruz.br)
  • Single nucleotide polymorphism-mediated translational suppression of endoplasmic reticulum mannosidase I modifies the onset of end-stage liver dise. (cdc.gov)
  • Single nucleotide polymorphism-mediated translational suppression of endoplasmic reticulum mannosidase I modifies the onset of end-stage liver disease in alpha1-antitrypsin deficiency. (cdc.gov)
  • alpha-Mannosidase (EC 3.2.1.24, alpha-D-mannosidase, p-nitrophenyl-alpha-mannosidase, alpha-D-mannopyranosidase, 1,2-alpha-mannosidase, 1,2-alpha-D-mannosidase, exo-alpha-mannosidase) is an enzyme involved in the cleavage of the alpha form of mannose. (wikipedia.org)
  • The primary metabolic defect responsible for these manifestations is the deficiency of the acidic α-mannosidase activity (α-D-mannoside mannohydrolase EC 3.2.1.24) (4,19,22) which results in the lysosomal accumulation of mannose-rich oligosaccharides in neural (23) and visceral tissues (1,22) and in the urine (1,20,21,32) of affected homozygous patients. (springer.com)
  • α-Mannosidosis (MIM 248500) is an autosomal recessive lysosomal storage disorder resulting from deficient activity of lysosomal α-mannosidase (LAMAN) (EC 3.2.1.24). (oup.com)
  • Comparison of these docked conformers with other rice GH1 structures revealed differences in the residues interacting with the catalytic acid/base between enzymes with and without β--D--mannosidase activity. (ntu.edu.sg)
  • Diseases caused by the loss of one or more enzymes involved in the hydrolysis of mannoside linkages (MANNOSIDASES). (ctdbase.org)
  • Complex N-glycans generated by enzymes such as a-mannosidase I inhibit the activation of naive T cells. (cusabio.com)
  • Thus complex N-glycans generated by enzymes such as alpha-mannosidase I inhibit the activation of naïve T cells. (fu-berlin.de)
  • Glycosylases are a group of enzymes that includes glucosidases, mannosidases and heparanases. (genecards.org)
  • Polyclonal antibodies specifically raised against recombinant Mns1 also immunoreacted with the soluble α 1,2-mannosidases E-I and E-II, indicating that Mns1 could share structural similarities with both soluble enzymes. (fiocruz.br)
  • Thereafter, N -glycans are modified by ER-resident enzymes glucosidase I (GI), glucosidase II (GII), UDP-glucose:glycoprotein glucosyltransferase (UGT) and mannosidase(s) and become functional appendices that determine the fate of the associated polypeptide. (biologists.org)
  • Avila, J.L. and Convit, J.: Characterization and properties of α-D-mannosidase of human polymorphonuclear leukocytes. (springer.com)
  • Purification and characterization of exo-α-d-mannosidase from a Cellulomonas sp. (elsevier.com)
  • A diagnosis can be established by measuring the activity of lysosomal alpha-mannosidase in leucocytes and screening for abnormal urinary excretion of mannose-rich oligosaccharides. (nih.gov)
  • Lysosomal α-mannosidase is an enzyme that acts to degrade N-linked oligosaccharides and hence plays an important role in mannose metabolism in humans and other mammalian species, especially livestock. (biomedcentral.com)
  • α-D-mannosidase is a lysosomal enzyme which is involved in the catabolism of N-linked glycoproteins through the sequential degradation of high-mannose, hybrid and complex oligosaccharides [ 1 ]. (biomedcentral.com)
  • We propose that this mannosidase homolog is a lectin that recognizes Man8GlcNAc2 oligosaccharides that serve as signals in the degradation pathway. (unibas.ch)
  • A defective alpha-mannosidase enzyme, which normally helps to break down complex sugars derived from glycoproteins in the lysosome , causes progressive lysosomal accumulation of mannose-rich oligosaccharides in all tissues, resulting in impaired cellular function and apoptosis (Figure 2). (wikipedia.org)
  • Cytosolic α -mannosidases are glycosyl hydrolases that participate in the catabolism of cytosolic free N -oligosaccharides. (microbiologyresearch.org)
  • Analysis of cytosolic free N -oligosaccharides revealed that cytosolic α 1,2-mannosidase E-I trims free Man 8 GlcNAc 2 isomer B into Man 7 GlcNAc 2 isomer B. This is believed to be the first report demonstrating the presence of soluble α 1,2-mannosidase from the glycosyl hydrolases family 47 in a cytosolic compartment of the cell. (microbiologyresearch.org)
  • β-Mannosidases are exo-acting glycoside hydrolases (GHs) that catalyse the removal of the nonreducing end β-D-mannose from manno-oligosaccharides or mannoside-substituted molecules. (semanticscholar.org)
  • Syntheses of alkyl β- d -mannopyranosides and β-1,4 linked oligosaccharides using β-mannosidase from Rhizopus niveus . (springer.com)
  • Support for a B2,5 transition state during enzymatic beta-mannosidase hydrolysis should also facilitate the design and exploitation of transition state mimics for the inhibition of retaining alpha-mannosidases--an area that is emerging for anticancer therapeutics. (rcsb.org)
  • Die Inhibition der alpha-Mannosidase-I-Aktivität führt zu einer höheren Frequenz an CD69+-T-Zellen, gesteigerten IL-2-Produktion und -Transkription. (fu-berlin.de)
  • To study the effect of inhibition of 6A8 alpha-mannosidase expression on adhesiveness among and E-cadherin expression on CNE-2L2 cells , and on metastasis of the tumors from the cells inoculated in nude mice . (bvsalud.org)
  • Inhibition of 6A8 alpha-mannosidase expression results in enhancement of cell - cell adhesion and of E-cadherin expression on CNE-2L2 cells . (bvsalud.org)
  • The defects in enzyme activity are primarily associated with genetic mutation of the genes that codes for a particular mannosidase isoenzyme. (ctdbase.org)
  • This biochemical test is a quantitative measurement of alpha-mannosidase enzyme activity and can be used as a 1st tier test for patients with a clinical suspicion of alpha-mannosidosis. (isinproduction.com)
  • Demonstration of deficient alpha-mannosidase enzyme activity is considered the gold standard to confirm a diagnosis of alpha-mannosidosis. (isinproduction.com)
  • Alpha mannosidosis is an autosomal recessive lysosomal storage disorder caused by a deficiency of alpha-mannosidase enzyme activity. (isinproduction.com)
  • When alpha-1,2-mannosidase enzyme activity was blocked, Treg retained their capacity to suppress T cell proliferation in vitro but were unable to bind to physiologically relevant ligands in vitro. (ox.ac.uk)
  • Orthologous to human MAN2B1 (mannosidase alpha class 2B member 1). (jax.org)
  • Here we present the analysis of 25 putative beta-mannosidase inhibitors, whose Ki values range from nanomolar to millimolar, on the Bacteroides thetaiotaomicron beta-mannosidase BtMan2A. (rcsb.org)
  • Click on the product name to view detailed information such as the chemical structure and specific chemical properties for each of our alpha-mannosidase Inhibitors. (scbt.com)
  • In stock alpha-mannosidase Inhibitors are available for immediate shipping. (scbt.com)
  • Functionalized pyrrolidine inhibitors of human type II alpha-mannosidases as anti-cancer agents: optimizing the fit to the active site. (expasy.org)
  • Beta-mannosidase is an enzyme with system name beta-D-mannoside mannohydrolase. (creative-enzymes.com)
  • Humans express the following three alpha-mannosidase isozymes: It can be utilized in experiments that determine the effects of the presence or absence of mannose on specific molecules, such as recombinant proteins that are used in vaccine development. (wikipedia.org)
  • High purity recombinant β-Mannosidase ( Cellilomonas fimi ) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. (megazyme.com)
  • The optimized conditions for overexpression of recombinant β-mannosidase enzyme were induction with 1 mM IPTG for 12 h at 37°C. The expressed β-mannosidase enzyme was purified to homogeneity by a combination of DEAE-ion exchange and size exclusion chromatography. (edu.pl)
  • There are two types: alpha-Mannosidase beta-Mannosidase A deficiency is associated with mannosidosis. (wikipedia.org)
  • Hultberg, B.: Properties of α-mannosidase in mannosidosis. (springer.com)
  • deficient in beta-mannosidosis) and nonlysosomal beta-mannosidase (neutral) activity. (curehunter.com)
  • The plasma alpha-mannosidase assay is a rapid and reliable screening test for the mannosidosis genotype and for detecting carrier animals. (monash.edu)
  • A comprehensive homology modeling study of all the wild-type and inherited mutations of lysosomal α-mannosidase in four different species, human, cow, cat and guinea pig, reveals a significant correlation between the severity of the genotype and the phenotype in α-mannosidosis. (biomedcentral.com)
  • Alpha-mannosidosis (AM) is a lysosomal storage disease , which may also be referred to as lysosomal acid hydrolase alpha-mannosidase or LAHAM. (symptoma.com)
  • Biochemicals that inhibit alpha-mannosidase have many applications in biochemical and physiological research. (scbt.com)
  • Cooper A, Hatton C, Thornley M, Sardharwalla IB (1988) Human β-mannosidase deficiency: biochemical findings in plasma, fibroblasts, white cells and urine. (edu.pl)
  • Mannan endo-1,4-β-mannosidases (1,4-β- D -mannanases) catalyze the random hydrolysis of β-1,4-mannosidic linkages in the main chain of β-mannans. (biomedcentral.com)
  • This study is the first report on the cloning and expression of a thermostable mannan endo-1,4-β-mannosidase from A. niger in Pichia pastoris . (biomedcentral.com)
  • The N -mannan core trimming properties of the purified enzyme E-I were consistent with its classification as a family 47 α 1,2-mannosidase. (microbiologyresearch.org)
  • Activity and capacity to utilize Saccharomyces cerevisiae mannan of the best α-mannosidase producer Aspergillus flavus Link 69. (springer.com)
  • We established another new rapid non-culture method for detection of C. trachomatis based on the measurement of α-mannosidase enzymatic activity in urogenital tract specimens. (sigmaaldrich.com)
  • It resembles a cascade of various enzymatic reactions, in which class I alpha−mannosidases play a central role. (fu-berlin.de)
  • Overall, the present study not only helps in understanding the catalytic mechanism of GH family 5 β-mannosidases, but also provides a basis for further enzymatic engineering of β-mannosidases and β-mannanases. (semanticscholar.org)
  • α-Mannosidase of genera Aspergillus and Rhizopus . (springer.com)
  • This enzyme, ERalpha-mannosidase I (ERManl), acts as a key timer for ER residence for newly synthesized glycoproteins by initiating a rate-limiting step leading to a cascade of interactions that ultimately leads to the targeting of terminally misfolded glycoproteins for retrotranslocation to the cytoplasm and proteasomal disposal in a process known as """"""""ER-associated degradation"""""""" (ERAD). (grantome.com)
  • 1,2-mannosidases that participate in the N -glycan trimming and ER-associated degradation of glycoproteins (Herscovics 1999a, b, Herscovics 2001, Helenius & Aebi 2004) belong to family 47 of glycosyl hydrolases. (fiocruz.br)
  • ER mannosidase I (ERManI) is a quality control factor that plays a critical role in the sorting and targeting of misfolded glycoproteins for proteasome-mediated degradation. (cdc.gov)
  • We have examined the expression of one of these genes, alpha-mannosidase. (asm.org)
  • High cell density or the presence of buffer conditioned by high-density cells was sufficient to induce expression of alpha-mannosidase, indicating that this is one of the prestarvation response genes. (asm.org)
  • This finding may indicate that the mammalian α‐mannosidases, which differ significantly in their substrate specificity, are coded for by evolutionarily unrelated genes, providing an attractive means of regulation and fine‐tuning oligosaccharide processing, not only at the enzymic but also at the transcriptional level. (elsevier.com)
  • We used the X-ray crystallographic structure of bovine lysosomal α-mannosidase as template, containing only two disulphide bonds and some ligands, to build structural models of wild-type structures with four disulfide linkages and all bound ligands. (biomedcentral.com)
  • Mannosidase Deficiency Diseases" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (childrensmercy.org)
  • This graph shows the total number of publications written about "Mannosidase Deficiency Diseases" by people in this website by year, and whether "Mannosidase Deficiency Diseases" was a major or minor topic of these publications. (childrensmercy.org)
  • Below are the most recent publications written about "Mannosidase Deficiency Diseases" by people in Profiles. (childrensmercy.org)
  • abstract = "A Gram-positive bacterium isolated from a soil sample was found to produce a high level of α-mannosidase in culture media. (elsevier.com)
  • This paper describes a manual fluorimetric method for the assay of acidic alpha-mannosidase activity in bovine plasma. (monash.edu)
  • β-mannosidase (EC 3.2.1.25, MANB) dissects the non-reducing end of N-linked mannose moieties of glycoproteins in eukaryotic cells. (edu.pl)
  • We previously demonstrated that alpha-1,2-mannosidase, an enzyme involved in the synthesis and processing of N-linked glycoproteins, is highly expressed in tolerant mice, in both graft infiltrating leukocytes and peripheral blood lymphocytes. (ox.ac.uk)
  • The mutations result in a beta-mannosidase enzyme with little or no activity, and interfere with the ability of the enzyme to perform its role in breaking down mannose-containing disaccharides. (medlineplus.gov)
  • mannosidases involved in Asn-linked glycan maturation in the mammalian secretory pathway. (grantome.com)
  • 1,2-mannosidases, which after the action of ER enzyme, trim three 1,2-linked mannose residues to generate the Man 5 GlcNAc 2 (M 5 ) intermediate required for the formation of the complex and hybrid N -glycans found in mammalian cells and filamentous fungi (Ichishima et al. (fiocruz.br)
  • Mannosidase is an enzyme which hydrolyses mannose. (wikipedia.org)
  • An inborn error of metabolism marked by a defect in the lysosomal isoform of BETA-MANNOSIDASE that results in lysosomal accumulation of mannose-rich intermediate metabolites containing 1,4-beta linkages. (curehunter.com)
  • Alpha-mannosidase cleaves the alpha form of mannose. (agrisera.com)
  • Beta-mannosidase is involved in the last step of this process, helping to break down complexes of two sugar molecules (disaccharides) containing a sugar molecule called mannose. (medlineplus.gov)
  • 1,2-mannosidases, that remove only one mannose residue from Man 9 GlcNAc 2 (M 9 ), generating the Man 8 GlcNAc 2 isomer B (M 8 B) (Ziegler & Trimble 1991, Tremblay & Herscovics 1999, Mora-Montes et al. (fiocruz.br)
  • Trp119, Asn260 and Glu380 in the β-mannosidase, which are involved in hydrogen-bond contacts with the -1 mannose, might be essential for exo catalytic activity. (semanticscholar.org)
  • Chester, M.A., Lundblad, A. and Masson, P.K.: The relationship between different forms of human α-mannosidase. (springer.com)
  • The DNA sequence was compared with reported human β-mannosidase DNA sequence and sixteen nucleotide differences were found. (edu.pl)
  • E-I corresponded to a polypeptide of 52 kDa that was associated with mannosidase activity and was recognized by an anti- α 1,2-mannosidase antibody. (microbiologyresearch.org)
  • We have previously reported the substrate specificity of the cytosolic α- D -mannosidase purified from rat liver using Man 9 GlcNAc, i.e. (biochemj.org)
  • Cytosolic class II α-mannosidase from T. maritima (TM1851), a family 38 glycoside hydrolase, was crystallized. (iucr.org)
  • Class I 1,2-α-mannosidase enzyme activitywas also detected in the T27K vaccine using the substrate, Man-α-1,2-Man-α-OCH 3 in a spectrophotometric assay. (elsevier.com)
  • Two affected siblings of Palestinian origin were homozygous for a mutation that causes a His→Leu replacement at a position which is conserved among class 2α-mannosidases from several species. (oup.com)
  • Two fold serial dilutions of α1-2,3 Mannosidase are incubated with 1 nmol AMC-labeled substrate in 1X GlycoBuffer 1, supplemented with 100 µg/ml BSA, in a 10 µl reaction. (neb.com)
  • In contrast to Bacteroidetes, these Bifidobacteria have a specialist and strain-specific β-mannosidase that contains three distinctive structural elements conferring high selectivity for Man-β-1,4-GlcNAc: a lid that undergoes conformational changes upon substrate binding, a tryptophan residue swapped between the two dimeric subunits to accommodate the GlcNAc moiety, and a Rossmann fold subdomain strategically located near to the active site pocket. (unicamp.br)
  • Structural insights into the substrate specificity and transglycosylation activity of a fungal glycoside hydrolase family 5 β-mannosidase. (semanticscholar.org)