The type species of ARENAVIRUS, part of the Old World Arenaviruses (ARENAVIRUSES, OLD WORLD), producing a silent infection in house and laboratory mice. In humans, infection with LCMV can be inapparent, or can present with an influenza-like illness, a benign aseptic meningitis, or a severe meningoencephalomyelitis. The virus can also infect monkeys, dogs, field mice, guinea pigs, and hamsters, the latter an epidemiologically important host.
A form of meningitis caused by LYMPHOCYTIC CHORIOMENINGITIS VIRUS. MICE and other rodents serve as the natural hosts, and infection in humans usually occurs through inhalation or ingestion of infectious particles. Clinical manifestations include an influenza-like syndrome followed by stiff neck, alterations of mentation, ATAXIA, and incontinence. Maternal infections may result in fetal malformations and injury, including neonatal HYDROCEPHALUS, aqueductal stenosis, CHORIORETINITIS, and MICROCEPHALY. (From Joynt, Clinical Neurology, 1996, Ch26, pp1-3)
Serum containing GAMMA-GLOBULINS which are antibodies for lymphocyte ANTIGENS. It is used both as a test for HISTOCOMPATIBILITY and therapeutically in TRANSPLANTATION.
Virus diseases caused by the ARENAVIRIDAE.
Surgical removal of the thymus gland. (Dorland, 28th ed)
Viruses whose taxonomic relationships have not been established.
Viruses whose genetic material is RNA.
The only genus in the family ARENAVIRIDAE. It contains two groups ARENAVIRUSES, OLD WORLD and ARENAVIRUSES, NEW WORLD, which are distinguished by antigenic relationships and geographic distribution.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
A programmed mutation process whereby changes are introduced to the nucleotide sequence of immunoglobulin gene DNA during development.
A transfer RNA which is specific for carrying leucine to sites on the ribosomes in preparation for protein synthesis.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
A contagious disease caused by canine adenovirus (ADENOVIRUSES, CANINE) infecting the LIVER, the EYE, the KIDNEY, and other organs in dogs, other canids, and bears. Symptoms include FEVER; EDEMA; VOMITING; and DIARRHEA.
The theory that T-cells monitor cell surfaces and detect structural changes in the plasma membrane and/or surface antigens of virally or neoplastically transformed cells.
Slender, cylindrical filaments found in the cytoskeleton of plant and animal cells. They are composed of the protein TUBULIN and are influenced by TUBULIN MODULATORS.
A multicentric, malignant neoplastic vascular proliferation characterized by the development of bluish-red cutaneous nodules, usually on the lower extremities, most often on the toes or feet, and slowly increasing in size and number and spreading to more proximal areas. The tumors have endothelium-lined channels and vascular spaces admixed with variably sized aggregates of spindle-shaped cells, and often remain confined to the skin and subcutaneous tissue, but widespread visceral involvement may occur. Kaposi's sarcoma occurs spontaneously in Jewish and Italian males in Europe and the United States. An aggressive variant in young children is endemic in some areas of Africa. A third form occurs in about 0.04% of kidney transplant patients. There is also a high incidence in AIDS patients. (From Dorland, 27th ed & Holland et al., Cancer Medicine, 3d ed, pp2105-7) HHV-8 is the suspected cause.
Nocodazole is an antineoplastic agent which exerts its effect by depolymerizing microtubules.
A microtubule subunit protein found in large quantities in mammalian brain. It has also been isolated from SPERM FLAGELLUM; CILIA; and other sources. Structurally, the protein is a dimer with a molecular weight of approximately 120,000 and a sedimentation coefficient of 5.8S. It binds to COLCHICINE; VINCRISTINE; and VINBLASTINE.
Antitumor alkaloid isolated from Vinca rosea. (Merck, 11th ed.)
Agents that interact with TUBULIN to inhibit or promote polymerization of MICROTUBULES.
Inflammation of the coverings of the brain and/or spinal cord, which consist of the PIA MATER; ARACHNOID; and DURA MATER. Infections (viral, bacterial, and fungal) are the most common causes of this condition, but subarachnoid hemorrhage (HEMORRHAGES, SUBARACHNOID), chemical irritation (chemical MENINGITIS), granulomatous conditions, neoplastic conditions (CARCINOMATOUS MENINGITIS), and other inflammatory conditions may produce this syndrome. (From Joynt, Clinical Neurology, 1994, Ch24, p6)
Bacterial infections of the leptomeninges and subarachnoid space, frequently involving the cerebral cortex, cranial nerves, cerebral blood vessels, spinal cord, and nerve roots.
Infection with CYTOMEGALOVIRUS, characterized by enlarged cells bearing intranuclear inclusions. Infection may be in almost any organ, but the salivary glands are the most common site in children, as are the lungs in adults.
A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, infecting the salivary glands, liver, spleen, lungs, eyes, and other organs, in which they produce characteristically enlarged cells with intranuclear inclusions. Infection with Cytomegalovirus is also seen as an opportunistic infection in AIDS.
Vaccines or candidate vaccines used to prevent infection with CYTOMEGALOVIRUS.
Administration of vaccines to stimulate the host's immune response. This includes any preparation intended for active immunological prophylaxis.
Layers of protein which surround the capsid in animal viruses with tubular nucleocapsids. The envelope consists of an inner layer of lipids and virus specified proteins also called membrane or matrix proteins. The outer layer consists of one or more types of morphological subunits called peplomers which project from the viral envelope; this layer always consists of glycoproteins.
A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, whose viruses have been isolated from lymphocytes. HERPESVIRUS 6, HUMAN is the type species.
The co-occurrence of pregnancy and an INFECTION. The infection may precede or follow FERTILIZATION.

Recognition of the major histocompatibility complex restriction element modulates CD8(+) T cell specificity and compensates for loss of T cell receptor contacts with the specific peptide. (1/1159)

Triggering of a T cell requires interaction between its specific receptor (TCR) and a peptide antigen presented by a self-major histocompatibility complex (MHC) molecule. TCR recognition of self-MHC by itself falls below the threshold of detection in most systems due to low affinity. To study this interaction, we have used a read-out system in which antigen-specific effector T cells are confronted with targets expressing high levels of MHC compared with the selecting and priming environment. More specifically, the system is based on CD8(+) T cells selected in an environment with subnormal levels of MHC class I in the absence of beta2-microglobulin. We observe that the MHC restriction element can trigger viral peptide-specific T cells independently of the peptide ligand, provided there is an increase in self-MHC density. Peptide-independent triggering required at least four times the natural in vivo level of MHC expression. Furthermore, recognition of the restriction element at expression levels below this threshold was still enough to compensate for lack of affinity to peptides carrying alanine substitutions in major TCR contact residues. Thus, the specificity in TCR recognition and T cell activation is fine tuned by the avidity for self-MHC, and TCR avidities for peptide and MHC may substitute for each other. These results demonstrate a functional role for TCR avidity for self-MHC in tuning of T cell specificity, and support a role for cross-reactivity on "self" during T cell selection and activation.  (+info)

Cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) can regulate dendritic cell-induced activation and cytotoxicity of CD8(+) T cells independently of CD4(+) T cell help. (2/1159)

The mechanisms that regulate the strength and duration of CD8(+) cytotoxic T cell activity determine the effectiveness of an antitumor immune response. To better understand the antitumor effects of anti-cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) antibody treatment, we analyzed the effect of CTLA-4 signaling on CD8(+) T cells in vitro and in vivo. In vitro, cross-linking of CTLA-4 on purified CD8(+) T cells caused decreased proliferative responses to anti-CD3 stimulation and rapid loss of activation marker expression. In vivo, blockade of CTLA-4 by neutralizing anti-CTLA-4 mAb greatly enhanced the accumulation, activation, and cytotoxic activity of CD8(+) T cells induced by immunization with Ag on dendritic cells (DC). This enhanced response did not require the expression of MHC class II molecules on DC or the presence of CD4(+) T cells. These results demonstrate that CTLA-4 blockade is able to directly enhance the proliferation and activation of specific CD8(+) T cells, indicating its potential for tumor immunotherapy even in situations in which CD4(+) T cell help is limited or absent.  (+info)

Use of a high-affinity peptide that aborts MHC-restricted cytotoxic T lymphocyte activity against multiple viruses in vitro and virus-induced immunopathologic disease in vivo. (3/1159)

Binding of a specific peptide(s) from a viral protein to major histocompatibility complex (MHC) class I molecules is a critical step in the activation of CD8(+) cytotoxic T lymphocytes (CTLs). Once activated, CTLs can cause lethal disease in an infected host, for example, by killing virus-containing ependymal and ventricular cells in the central nervous system or viral protein-expressing beta cells in the pancreatic islets of Langerhans. Here we describe the usage of a designed (not natural) high-affinity peptide to compete with viral peptide(s)-MHC binding. This peptide blocks virus-induced CTL-mediated disease both in the CNS and in the pancreatic islets in vivo. Further, the blocking peptide aborts MHC-restricted killing of target cells by CTLs generated to three separate viruses: lymphocytic choriomeningitis virus, influenza virus, and simian virus 40.  (+info)

In vivo selection of neutralization-resistant virus variants but no evidence of B cell tolerance in lymphocytic choriomeningitis virus carrier mice expressing a transgenic virus-neutralizing antibody. (4/1159)

B cell tolerance is maintained by active deletion and functional anergy of self-reactive B cells depending on the time, amount, and site of the self-antigen expression. To study B cell tolerance toward a transplacentally transmitted viral Ag, we crossed transgenic mice expressing the mu heavy and the kappa light chain of the lymphocytic choriomeningitis virus (LCMV)-neutralizing mAb KL25 (HL25-transgenic mice) with persistently infected LCMV carrier mice. Although HL25-transgenic LCMV carrier mice exhibited the same high virus titers as nontransgenic LCMV carrier mice, no evidence for B cell tolerance was found. In contrast, enhanced LCMV-neutralizing Ab titers were measured that, however, did not clear the virus. Instead, LCMV isolates from different tissues turned out to be neutralization resistant Ab escape variants expressing different substitutions of amino acid Asn119 of the LCMV-glycoprotein 1 that displays the neutralizing B cell epitope. Virus variants with the same mutations were also selected in vitro in the presence of the transgenic mAb KL25 confirming that substitutions of Asn119 have been selected by LCMV-neutralizing Abs. Thus, despite abundant expression of viral neo-self-antigen in HL25-transgenic LCMV carrier mice, transgenic B cells expressing LCMV-neutralizing Abs were rather stimulated than tolerized and neutralization resistant Ab escape variants were selected in vivo.  (+info)

Identification of MaTu-MX agent as a new strain of lymphocytic choriomeningitis virus (LCMV) and serological indication of horizontal spread of LCMV in human population. (5/1159)

In this study we elucidated the molecular character of MaTu-MX, previously described as an unusual transmissible agent. Amino acid sequencing of peptides generated from a 58-kDa MX-related protein purified from MaTu human carcinoma cells allowed us to identify it as a nucleoprotein (NP) of lymphocytic choriomeningitis virus (LCMV). Northern blot analysis detected LCMV-specific RNAs in MaTu cells. Comparative immunoprecipitations showed cross-reactivity between NP of LCMV strain WE and MX NP. Using RT-PCR, we have cloned MX NP cDNA. According to sequence comparison, MX LCMV is as closely related to both LCMV strains WE and Armstrong as these strains are to one another. Based on this finding we propose that MX is a new strain of LCMV. We also showed that the stability of MX NP in MaTu cells is very high and that the virus is transmissible by cell-to-cell contact or by cell-free extract to human HeLa and monkey Vero cells, but not to human AGS, canine MDCK, mouse NIH 3T3, and hamster CHO cells. Finally, employing MX LCMV NP in immunoprecipitation and solid-phase radioimmunoassay, we found 37.5% prevalence of anti-LCMV antibodies in human sera, suggesting possible horizontal spread of the virus in the human population.  (+info)

Two roads diverged: interferon alpha/beta- and interleukin 12-mediated pathways in promoting T cell interferon gamma responses during viral infection. (6/1159)

Viral infections induce CD8 T cell expansion and interferon (IFN)-gamma production for defense, but the innate cytokines shaping these responses have not been identified. Although interleukin (IL)-12 has the potential to contribute, IL-12-dependent T cell IFN-gamma has not been detected during viral infections. Moreover, certain viruses fail to induce IL-12, and elicit high levels of IFN-alpha/beta to negatively regulate it. The endogenous factors promoting virus-induced T cell IFN-gamma production were defined in studies evaluating CD8 T cell responses during lymphocytic choriomeningitis virus infections of mice. Two divergent supporting pathways were characterized. Under normal conditions of infections, the CD8 T cell IFN-gamma response was dependent on endogenous IFN-alpha/beta effects, but was IL-12 independent. In contrast, in the absence of IFN-alpha/beta functions, an IL-12 response was revealed and substituted an alternative pathway to IFN-gamma. IFN-alpha/beta-mediated effects resulted in enhanced, but the alternative pathway also promoted, resistance to infection. These observations define uniquely important IFN-alpha/beta-controlled pathways shaping T cell responses during viral infections, and demonstrate plasticity of immune responses in accessing divergent innate mechanisms to achieve similar ultimate goals.  (+info)

A novel approach to visualize polyclonal virus-specific CD8 T cells in vivo. (7/1159)

Recent technical breakthroughs in generating soluble MHC class I-peptide tetramers now allow the direct visualization of virus-specific CD8 T cells after infection in vivo. However, this technique requires the knowledge of the immunodominant viral epitopes recognized by T cells. Here, we describe an alternative approach to visualize polyclonal virus-specific CD8 T cells in vivo using a simple adoptive transfer system. In our approach, C57BL/6 (Thy1.2) mice were infected with lymphocytic choriomeningitis virus, vesicular stomatitis virus, or vaccinia virus to induce virus-specific memory T cells. Tracer T cells (2 x 106) from these virus-immune mice were adoptively transferred into nonirradiated (C57BL/6 x B6.PL-Thy-1a)F1 mice. After infection of the F1-recipient mice with the appropriate virus, the transferred cells expanded vigorously, and on day 8 postinfection 60-80% of total CD8 T cells were of donor T cell origin. Under the same conditions memory CD4 T cells gave rise to at least 10 times less cell numbers than memory CD8 T cells. The transfer system described here not only allows to visualize effector and memory CD8 T cells in vivo but also to isolate them for further in vitro characterization without knowing the epitopes recognized by these Ag-specific CD8 T cells.  (+info)

Synergism for cytokine-mediated disease during concurrent endotoxin and viral challenges: roles for NK and T cell IFN-gamma production. (8/1159)

Viral infections in humans or mice can result in increased sensitivity to challenges with bacteria, bacterial products, or cytokine administration. During lymphocytic choriomeningitis virus infections, mice are more sensitive to the lethal effects of bacterial endotoxin LPS, and in the experiments reported here, were observed at up to 10-fold lower doses in infected than in uninfected mice. The mechanisms responsible for heightened susceptibility under these conditions were evaluated. Kinetic studies demonstrated that virus-infected mice had 3- to 50-fold increases over uninfected mice in peak serum TNF, IL-12, and IFN-gamma levels after LPS administration. All three cytokines contributed to lethality during dual challenge, because neutralization of any one of the factors protected from death. Production of TNF was not dependent on either NK or T cells. In contrast, these populations were the predominant sources of IFN-gamma, as determined by lack of detectable IFN-gamma production in NK and T cell-deficient mice and by intracellular cytokine expression in the cell subsets. Concordant with the demonstrations that both cell populations produced IFN-gamma and that this factor was critical for lethality, removal of either subset alone was not sufficient to protect mice from death resulting from dual challenges. Increased resistance required absence of both cell subsets. Taken together, the data show that during viral infections, the normally protective immune responses can profoundly modify reactions to secondary heterologous challenges, to result in dysregulated cytokine expression and consequent heightened detrimental effects.  (+info)

Lymphocytic choriomeningitis (LCM) is a rodent-borne viral infectious disease that presents as aseptic meningitis, encephalitis or meningoencephalitis. Its causative agent is the lymphocytic choriomeningitis virus (LCMV), a member of the family Arenaviridae. The name was coined by Charles Armstrong in 1934. The Gale Encyclopedia of Medicine defines lymphocytic choriomeningitis (LCM) as a viral infection of the membranes surrounding the brain and spinal cord and of the cerebrospinal fluid. The name is based on the tendency of an individual to have abnormally high levels of lymphocytes during infection. Choriomeningitis is cerebral meningitis in which there is marked cellular infiltration of the meninges, often with a lymphocytic infiltration of the choroid plexuses. There are several strains of LCM virus, among which the most widely used are LCMV Armstrong and LCMV Clone 13. Armstrong is the original virus strain which was isolated from the brain by Charles Armstrong in 1934. It triggers a ...
Isolates of lymphocytic choriomeningitis virus (LCMV) that elicit a cytotoxic T-lymphocyte response (CTL+) have been compared with isolates that suppress the CTL response (CTL-) in an effort to map this phenotype. A single amino acid change in the glycoprotein of the LCMV Armstrong (ARM) strain is consistently associated with the CTL- trait and the ability of the virus to persist (P+). The CTL+ P- parental strain spontaneously gives rise to CTL- P+ variants within lymphoid tissues of mice persistently infected from birth. To map the structural basis of the phenotype, the complete RNA sequence of LCMV ARM 53b (CTL+) was compared with that of its variant ARM clone 13 (CTL-). Differences in 5 of 10,600 nucleotides were found. Three changes are noted in the large L RNA segment, and two are noted in the small S RNA segment. Only two of the changes distinguishing CTL+ from CTL- isolates affect amino acid coding: lysine to glutamine at amino acid 1079 of the polymerase protein, and phenylalanine to ...
Lymphocytic choriomeningitis virus (LCMV) is a single-stranded RNA virus that belongs to the family Arenaviridae (so named because of its appearance on electron microscopy, which resembles grains of sand). Other members of this family include Lassa virus and the Tacaribe group.
Analyses with segmental reassortants of lymphocytic choriomeningitis virus (LCMV) RNA have shown that cytotoxic T lymphocytes (CTL) are induced by and recognize proteins encoded by the viral short segment, which specifies two virus structural proteins, glycoprotein (GP) and nucleoprotein (NP). Expression of cDNA copies of these genes in vaccinia virus vectors demonstrates that C57BL/6 (H2bb) mice mount significant CTL responses to both GP and NP. We have used LCMV-specific H2bb-restricted CTL clones and a family of serial C-terminal truncations of the LCMV GP expressed in vaccinia virus to map the precise specificities of the anti-GP clones. Of the 18 CTL clones studied, 1 recognizes NP and the other 17 recognize GP. The reactivities of 14 of the 17 anti-GP CTL clones against the deleted GP molecules have been fully characterized, and two clear patterns of anti-GP activity have emerged, defining at least two CTL epitopes. The first epitope, recognized by only two of the clones, lies within GP ...
Goldsmith, Cynthia S.; Ksiazek, Thomas G.; Rollin, Pierre E.; Comer, James A.; Nicholson, William L.; Peret, Teresa C.T.; Erdman, Dean D.; Bellini, William J.; Harcourt, Brian H.; Rota, Paul A.; Bhatnagar, Julu; Bowen, Michael D.; Erickson, Bobbie R.; McMullan, Laura K.; Nichol, Stuart T.; Shieh, Wun-Ju; Paddock, Christopher D.; Zaki, Sherif R ...
We determined the prevalence of infection with lymphocytic choriomeningitis virus (LCMV) among small mammals in northern Italy and analyzed long-term dynamics of LCMV in a rodent population in the province of Trento. LCMV is circulating among the most widespread and common wild rodent species in this area (Apodemus flavicollis, Myodes glareolus, and Microtus arvalis); overall prevalence is 6.8%. During 2000-2006, intensive monitoring of LCMV in a population of yellow-necked mice (A. flavicollis) showed a positive correlation between prevalence of infection and rodent density. At the individual level, weight and sex appeared to correlate with antibody prevalence, which suggests that horizontal transmission of LCMV occurs principally among heavier, older males and occurs during fighting. Isolation and genetic characterization of this virus will be the crucial next steps for a better understanding of its ecology.
Persistent infection of C3H/St mice with certain strains of lymphocytic choriomeningitis virus (LCMV) causes a growth hormone (GH) deficiency syndrome (GHDS) manifested as growth retardation and hypoglycemia. Infected mice show high levels of viral replication in the GH-producing cells in the anterior pituitary leading to decreased synthesis of GH mRNA and protein despite the absence of detectable virus-induced cell structural damage. Virus clones isolated from the GHDS-negative LCMV WE strain can cause the disease, while others cannot. The genetic basis of this phenotypic difference is a nucleotide substitution resulting in a single amino acid difference in the viral glycoprotein. Reassortant studies indicate that the single amino acid substitution (Ser-153 to Phe) is sufficient to allow infection of the GH-producing cells and cause GHDS. These results show that a single change in the genome can affect viral pathogenicity by altering the tropism of the virus.
In Vivo Selection of a Lymphocytic Choriomeningitis Virus Variant That Affects Recognition of the GP33-43 Epitope by H-2Db but Not H-2Kb ...
Lymphocytic choriomeningitis virus RNA-directed RNA polymerase L (L) datasheet and description hight quality product and Backed by our Guarantee
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TY - JOUR. T1 - Effect of Chronic Viral Infection on Epitope Selection, Cytokine Production, and Surface Phenotype of CD8 T Cells and the Role of IFN-γ Receptor in Immune Regulation. AU - Tewari, Kavita. AU - Sacha, Jonah. AU - Gao, Xiaoyan. AU - Suresh, M.. PY - 2004/2/1. Y1 - 2004/2/1. N2 - Regulation of CD8 T cell responses in chronic viral infections is not well understood. In this study, we have compared the CD8 T cell responses to immunodominant and subdominant epitopes during an acute and a chronic lymphocytic choriomeningitis virus (LCMV) infection in mice. The epitope hierarchy of the primary CD8 T cell response was similar in acute and chronic LCMV infections. However, strikingly, the epitope hierarchy of the primary CD8 T cell response was conserved in the T cell memory only in an acute but not in a chronic LCMV infection. Interestingly, in an acute infection, increasing the viral dose caused significant changes in the epitope hierarchy of the LCMV-specific memory CD8 T cell pool, ...
THE interaction of lymphocytic choriomeningitis (LCM) virus with mouse tissues has been discussed recently1-5. The fulminating disease, which occurs in adult mice after intracerebral inoculation with LCM virus, can be ameliorated by several treatments known to impair the immune response of the host, such as X-rays6, amethopterin7, thymectomy8 and anti-lymphocyte serum9. The pathology of the disease10 supports the current view that an immune reaction by the host against the virus or a virus product, located in or on infected cells, plays an essential part in its pathogenesis. A similar mechanism may be important in the chronic autoimmune-like disease in virus carrier mice2,5. Further elucidation of this disease mechanism depends on understanding the nature of the immune response and its target. A question of considerable interest is what the consequences might be for virus infected cells in the event of an immune response against the virus or a virus product. Here we report preliminary experiments
Summary As shown previously, after inoculation into the footpad of a mouse the lymphocytic choriomeningitis (LMC) virus multiplies locally. Beginning on day 6 or 7 after infection, the foot undergoes a delayed-type hypersensitivity (DTH) reaction which consists of two distinct phases that are mediated by CD8+ cells and CD4+ cells, respectively, and at about the same time the virus is eliminated. In general, for terminating infection of the mouse with LCM virus the CD8+ cytotoxic/suppressive T lymphocyte (CTL) is essential; we have now determined the cells that mediate control of the virus in a tissue undergoing a specific DTH reaction. Depletion, in infected mice, of all T lymphocytes by treatment with anti-Thy-1 monoclonal antibody prevented virus elimination from the foot, and the same was true when the CD8+ CTLs were removed. Depletion of the CD4+ helper/suppressor subset only marginally impaired the ability of the mice to rid themselves of the virus. The conclusion that here too the principal
The main reservoir of LCMV, which belongs to the arenaviruses, is the house mouse. Cells infected with LCMV express antigens and are recognised by cytotoxic T lymphocytes. This lymphocyte activity also makes the blood-brain barrier permeable resulting in meninges and neurons being damaged. Infection of adult mice leads to choriomeningitis. In contrast, an intrauterine or neonatal infection generally causes an asymptomatic chronic carrier state in mice, with such animals forming immune complexes in the course of their lifetime that lead to glomerulonephritis. In guinea pigs and hamsters, LCMV infections often progress subclinically, however, conjunctivitis, blepharitis, respiratory symptoms, tremor, seizures and paralysis have been described. LCMV is transmitted diaplacentally and with all secretions and excretions. In humans, LCMV rarely leads to choriomeningitis; the infection is usually asymptomatic or shows mild, flu-like symptoms. An infection in the second part of pregnancy can cause severe ...
To establish if a correlation exists between the expansion of GP33-specific cells observed by flow cytometry and GP33-specific cytotoxicity, direct CTL assays were carried out using spleen cells from the mice described above. CTL activity mediated by antigen-specific CD8+ cells is usually difficult to measure at day 3 after infection since LCMV induces, at this early time point, strong NK cell activity that lyses both antigen-pulsed and unpulsed target cells. To circumvent this problem, direct CTL assays were carried out by adding a twofold excess of unlabeled NK-sensitive target cells (YAC-1 cells) to assay wells as cold-target competitors. Inclusion of cold YAC-1 cells abrogated lysis of chromium-labeled MC57 target cells by NK cells from the spleens of mice that had been injected 48 h previously with poly-IC (data not shown). No inhibition of antigen-specific CTLs was observed when YAC-1 cells were included as cold-target competitors in a direct CTL assay using spleen cells from mice that had ...
During chronic stimulation, CD8+ T cells acquire an exhausted phenotype characterized by expression of inhibitory receptors, down-modulation of effector function, and metabolic impairments. T cell exhaustion protects from excessive immunopathology but limits clearance of virus-infected or tumor cells. We transcriptionally profiled antigen-specific T cells from mice infected with lymphocytic choriomeningitis virus strains that cause acute or chronic disease. T cell exhaustion during chronic infection was driven by high amounts of T cell receptor (TCR)-induced transcription factors IRF4, BATF, and NFATc1. These regulators promoted expression of inhibitory receptors, including PD-1, and mediated impaired cellular metabolism. Furthermore, they repressed the expression of TCF1, a transcription factor required for memory T cell differentiation. Reducing IRF4 expression restored the functional and metabolic properties of antigen-specific T cells and promoted memory-like T cell development. These ...
Three clusters of organ transplant-associated lymphocytic choriomeningitis virus (LCMV) transmissions have been identified in the United States; 9 of 10 recipients died. In February 2011, we identified a fourth cluster of organ transplant-associated LCMV infections. Diabetic ketoacidosis developed in the organ donor in December 2010; she died with generalized brain edema after a short hospitalization. Both kidneys, liver, and lung were transplanted to 4 recipients; in all 4, severe posttransplant illness developed; 2 recipients died. Through multiple diagnostic methods, we identified LCMV infection in all persons, including in at least 1 sample from the donor and 4 recipients by reverse transcription PCR, and sequences of a 396-bp fragment of the large segment of the virus from all 5 persons were identical. In this cluster, all recipients developed severe illness, but 2 survived. LCMV infection should be considered as a possible cause of severe posttransplant illness ...
Lymphocytic choriomeningitis: Lymphocytic choriomeningitis, inflammation of the meninges (membranes covering the central nervous system) and choroid plexus (an area of the brain that regulates the
(a) Schematic view of acute and chronic LCMV infections including virus and cytotoxic T lymphocyte (CTL) dynamics. (b) Representation of the CTL-induced immunop
Katrina Armstrong, MD, MSCE, a world-renowned investigator in the areas of medical decision-making, quality of care and cancer prevention and outcomes, has been selected as the MGHs next physician-in-chief of the MGH Department of Medicine. Armstrong will succeed internationally regarded physician-scientist Dennis Ausiello, MD, who has led the department since 1996.. Armstrong, who currently serves as chief of the Division of General Medicine and professor of Medicine and Obstetrics and Gynecology at the Perelman School of Medicine at the University of Pennsylvania, will start in the new position April 15. We are at a time of unparalleled opportunity to advance science, education and clinical care to benefit our patients and our communities, says Armstrong. MGH is an extraordinary institution with a long tradition of leadership in internal medicine, and I am honored to become part of that tradition.. Armstrong is a graduate of Yale University and Johns Hopkins University School of Medicine ...
Armstrong was promoted to the rank of Lieutenant with seniority of 13 November, 1892.[1] He married Ethel M. Heath on 23 February, 1893 at Kensington.[2] He was appointed in command of the destroyer Lynx on 3 August, 1897.[3] On 15 October, 1897, Armstrong was tried at a Court Martial for negligently stranding Lynx while following Thrasher in dense fog off the Cornish coast. He was acquitted.[4][5][6][7] In September, 1902, Armstrong was credited with the cleaver capture of a pair of pirate dhows at Bussorah.[8] He was promoted to the rank of Commander dated 1 January, 1903.[9] After commanding Lively in the 1903 manoeuvres, Armstrong undertook a war course and a senior officers gunnery course and was then appointed to the second class protected cruiser Challenger on 3 May, 1904. Upon paying her off on 20 July, 1906, he was appointed to Hyacinth, to assume command, temporarily for one month from 20 December, 1906.[10] Armstrong was promoted to the rank of Captain on 31 December, 1908.[11] On ...
Armstrong was promoted to the rank of Lieutenant with seniority of 13 November, 1892.[1] He married Ethel M. Heath on 23 February, 1893 at Kensington.[2] He was appointed in command of the destroyer Lynx on 3 August, 1897.[3] On 15 October, 1897, Armstrong was tried at a Court Martial for negligently stranding Lynx while following Thrasher in dense fog off the Cornish coast. He was acquitted.[4][5][6][7] In September, 1902, Armstrong was credited with the cleaver capture of a pair of pirate dhows at Bussorah.[8] He was promoted to the rank of Commander dated 1 January, 1903.[9] After commanding Lively in the 1903 manoeuvres, Armstrong undertook a war course and a senior officers gunnery course and was then appointed to the second class protected cruiser Challenger on 3 May, 1904. Upon paying her off on 20 July, 1906, he was appointed to Hyacinth, to assume command, temporarily for one month from 20 December, 1906.[10] Armstrong was promoted to the rank of Captain on 31 December, 1908.[11] On ...
tags: hamster, PetSmart, lawsuit, lymphocytic choriomeningitis virus, LCMV, zoonosis Portrait of a murderer: A Siberian dwarf hamster, Phodopus sungorus. Orphaned image. I just learned that a lawsuit was recently filed in Massachusetts Superior Court on behalf of a man who died one month after receiving a transplanted liver that was later determined to be infected…. ...
Summary A quantal microassay for the titration of LCM virus strains is described. It is based on the detection of virus-specific complement-fixing antigen in the medium of infected L cell microcultures.
Microbial infection) Acts as a receptor for Japanese encephalitis virus (PubMed:24623090). Acts as a receptor for ebolavirus (PubMed:16051304). Acts as a receptor for SARS coronavirus/SARS-CoV (PubMed:16051304). Acts as a receptor for lassa virus and Lymphocytic choriomeningitis virus glycoprotein (PubMed:22156524, PubMed:22673088 ...
TY - JOUR. T1 - Viral immune evasion due to persistence of activated T cells without effector function. AU - Zajac, Allan J.. AU - Blattman, Joseph N.. AU - Murali-Krishna, Kaja. AU - Sourdive, David J.D.. AU - Suresh, M.. AU - Altman, John D.. AU - Ahmed, Rafi. PY - 1998/12/1. Y1 - 1998/12/1. N2 - We examined the regulation of virus-specific CD8 T cell responses during chronic lymphocytic choriomeningitis virus (LCMV) infection of mice. Our study shows that within the same persistently infected host, different mechanisms can operate to silence antiviral T cell responses; CD8 T cells specific to one dominant viral epitope were deleted, whereas CD8 T cells responding to another dominant epitope persisted indefinitely. These virus- specific CD8 T cells expressed activation markers (CD69(hi), CD44(hi), CD62L(lo)) and proliferated in vivo but were unable to elaborate any antiviral effector functions. This unresponsive phenotype was more pronounced under conditions of CD4 T cell deficiency, ...
Lymphocytic Choriomeningitis (LCM) Lymphocytic choriomeningitis, or LCM, is a rodent-borne viral infectious disease caused by lymphocytic choriomeningitis virus (LCMV), a member of the family Arenaviridae that was initially isolated in 1933. The primary host of LCMV is the common house mouse, Mus musculus. Infection in house mouse populations may vary by geographic location, though it is estimated that 5% of house mice throughout the United States carry LCMV and are able to transmit virus for the duration of their lives without showing any sign of illness. Other types of rodents, such as hamsters, are not the natural reservoirs but can become infected with LCMV from wild mice at the breeder, in the pet store, or home environment. Humans are more likely to contract LCMV from house mice, but infections from pet rodents have also been reported.. LCMV infections have been reported in Europe, the Americas, Australia, and Japan, and may occur wherever infected rodent hosts of the virus are found. The ...
TY - JOUR. T1 - 4-1BB signaling synergizes with programmed death ligand 1 blockade to augment CD8 T cell responses during chronic viral infection. AU - Vezys, Vaiva. AU - Penaloza-Macmaster, Pablo. AU - Barber, Daniel L.. AU - Ha, Sang Jun. AU - Konieczny, Bogumila. AU - Freeman, Gordon J.. AU - Mittler, Robert S.. AU - Ahmed, Rafi. PY - 2011/8/15. Y1 - 2011/8/15. N2 - Previous studies have identified the inhibitory role that the programmed death 1 (PD-1) pathway plays during chronic infection. Blockade of this pathway results in rescue of viral-specific CD8 T cells, as well as reduction of viral loads in mice chronically infected with lymphocytic choriomeningitis virus (LCMV). We tested the effect of combining PD ligand 1 (PD-L1) blockade with an agonistic regimen that induces 4-1BB costimulation during chronic LCMV infection. There is a boosting effect in the rescue of LCMV-specific CD8 T cell responses after dual treatment with PD-L1 blockade and 4-1BB agonistic Abs when the amount and timing ...
Persistent viral infections can interfere with FcγR-mediated antibody effector functions by excessive immune complex (IC) formation, resulting in resistance to therapeutic FcγR-dependent antibodies. We and others have previously demonstrated that mice persistently infected with lymphocytic choriomeningitis virus (LCMV) are resistant to a wide range of depleting antibodies due to excessive IC formation. Here, we dissect the mechanisms by which two depleting antibodies overcome the obstacle of endogenous ICs and achieve efficient target cell depletion in persistently infected mice. Efficient antibody-mediated depletion during persistent LCMV infection required increased levels of antibody bound to target cells or use of afucosylated antibodies with increased affinity for FcγRs. Antibodies targeting the highly expressed CD90 antigen or overexpressed human CD20 efficiently depleted their target cells in naïve and persistently infected mice, whereas antibodies directed against less abundant ...
Jan. 2001-present SCIENTIFIC PUBLICATIONS: 1 Beck,M.L., S.H. Butch, W.R. Armstrong, and H.A. Oberman. An Auto-antibody with U- Specificity in a Patient with Myasthenia Gravis. Transfusion, 12:280:1972. 2. VanKirk,I.E., A.B. Simon, and W.R. Armstrong. Candida Myocarditis Causing Complete Heart Block. JAMA 25, Feb74, p.931-33. 3. Amistrong,W.R. and E.W. Catalano. The PAS Stain in Acute Leukemia - Increased Sensitivity with Diastase. Am.J.Clin.Path. 72:652, 1979. 4. Godder,K, Pati,AR, Abhyankar,SH, Lamb,LS, Armstrong,W and Henslee-Downey, J: De-novo Chronic Graft versus Host Disease Presenting as Hemolytic Anemia Following Partially Mismatched Related Donor Bone Marrow Transplant. Bone Marrow Transplant 19:813-817, 1997. 5. Thurer,Robert L, Luban,N., AuBuchonjP, et al: Universal WBC Reduction. Letters to the Editor, Transfusion 40:751-2, June 2000. PRESENTATIONS: 1 Armstrong,W.R. Fibrous Histiocytic Neoplasms. Department of Postgraduate Medicine, University of Michigan, 4/73. M.R. Abell, MD, ...
Transfer of photosynthetic NADP+/NADPH recycling activity to a porous metal oxide for highly specific, electrochemically-driven organic synthesis. B. Siritanaratkul, C. F. Megarity, T. G. Roberts, T. O. M. Samuels, M. Winkler, J. H. Warner, T. Happe and F. A. Armstrong, Chem. Sci, 8, 4579-4586 (2017). Importance of the Active Site Canopy Residues in an O2-tolerant [NiFe]-hydrogenase. E. J. Brooke, R. M. Evans, S. T. A. Islam, G. M. Roberts, S. A. M. Wehlin, S. B. Carr, S. E. V. Phillips and F. A. Armstrong. Biochemistry 56, 132-142 (2017).. Electrochemical investigations of the mechanism of assembly of the active-Site H-cluster of [FeFe]-Hydrogenases. C. F. Megarity, J. Esselborn, S. V. Hexter, F. Wittkamp, U.-P. Apfel, T. Happe and F. A. Armstrong. J. Amer. Chem. Soc. 138, 15227-15233 (2016).. Catalysis of solar hydrogen production by iron atoms on the surface of Fe-doped silicon carbide. Z. Wang and F. A. Armstrong. Catalysis Science and Technology, 6, 7038 - 7041 (2016).. Guiding Principles ...
AUSTIN, Texas (AP) - Lance Armstrong is facing more doping allegations just a few months after he thought he had finally put them to rest. Although federal investigators in February closed a two-year investigation without bringing criminal charges, the U.S. Anti-Doping Agency has filed new doping charges that could strip the seven-time Tour de France winner of his victories in cyclings premier race. Armstrong insists he is innocent. I have never doped, and, unlike many of my accusers, I have competed as an endurance athlete for 25 years with no spike in performance, passed more than 500 drug tests and never failed one, Armstrong said in a statement. Any fair consideration of these allegations has and will continue to vindicate me. The move by USADA immediately bans him from competing in triathlons, which he turned to after he retired from cycling last year. Armstrong has been dogged by doping allegations since his first Tour victory in 1999, but had hoped his fight to be viewed as a clean
The primary finding of this study was the emergence of a distinct A-to-G/U-to-C hypermutation pattern in the single-stranded RNA genome of LCMV both in cell culture and in the organs of infected mice. This pattern is probably due to unspecific deamination by ADAR1-L, which was found to be upregulated during infection of cells and mice with LCMV. Furthermore, these A-to-G mutations lead to a high percentage of nonfunctional viral protein, thereby weakening viral fitness.. The A-to-G hypermutation pattern observed in this study was most likely attributed to RNA editing and not to errors of the viral polymerase. The hypermutation pattern was not observed 2 days after infection in L929 cells. In mice at days 5 and 8 p.i., the dominance of A-to-G mutations is also reduced compared to that at day 4, which is consistent with lower ADAR1-L expression at these time points. Furthermore, although viral RNA polymerases lack 3′-to-5′ proofreading activity, they have never been reported to exhibit a ...
Dendritic cells (DC) play a key role in the initiation of T cell-mediated immune responses and may therefore be successfully used in antiviral and antitumor vaccination strategies. Because both strength and duration of an immune response determines the outcome of a vaccination protocol, we evaluated the life span of DC-induced antiviral CTL memory against systemic and peripheral challenge infections with lymphocytic choriomeningitis virus (LCMV). We found that expansion and activation of CTL by DC was transient. Protection against systemic LCMV infection after DC immunization was relatively long-lived (,60 days), whereas complete protection against peripheral infection via intracerebral infection or infection into the footpad with LCMV, where rapid recruitment of effector T cells to the site of infection and elimination of viral pathogen plays a major role, was short-lived (,30 days). Protective immunity was most efficiently restored by administration of antigenic peptides via DC, rather than in ...
Dendritic cells (DCs) comprise the major antigen-presenting cells (APCs) of the host, uniquely programmed to stimulate immunologically naïve T lymphocytes. Viruses that can target and disorder the...
Persistierende virale Infektionen wie z.B. Hepatitis C und HIV stellen wegen des Trägertums durch Tiere oder Menschen ein Virus-Reservoir dar, welches eine zunehmende Gesundheitsbedrohung bedeutet. Besonders im Bereich der Impfung, aber ebenso hinsichtlich Therapie der Infektion selbst und ihrer Langzeitkomplikationen, sind die Erfolge bescheiden oder unzureichend. Geläufige Konzepte und ...
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Infections induce complex host responses linked to antiviral defense, inflammation, and tissue damage and repair. We hypothesized that the liver, as a central metabolic hub, may orchestrate systemic metabolic changes during infection. We infected mice with chronic lymphocytic choriomeningitis virus (LCMV), performed RNA sequencing and proteomics of liver tissue, and integrated these data with serum metabolomics at different infection phases. Widespread reprogramming of liver metabolism occurred early after infection, correlating with type I interferon (IFN-I) responses. Viral infection induced metabolic alterations of the liver that depended on the interferon alpha/beta receptor (IFNAR1). Hepatocyte-intrinsic IFNAR1 repressed the transcription of metabolic genes, including Otc and Ass1, which encode urea cycle enzymes. This led to decreased arginine and increased ornithine concentrations in the circulation, resulting in suppressed virus-specific CD8+ T cell responses and ameliorated liver ...
Infections induce complex host responses linked to antiviral defense, inflammation, and tissue damage and repair. We hypothesized that the liver, as a central metabolic hub, may orchestrate systemic metabolic changes during infection. We infected mice with chronic lymphocytic choriomeningitis virus …
Adoptive immunization of syngeneic, immunosuppressed recipients infected with lymphocytic choriomeningitis (LCM) virus causes fatal neurologic disease within 2 to 4 days of cell transfer, providing that donors are sampled when the in vitro 51Cr release assay shows maximal specific activity of sensitized thymus-derived lymphocytes (T cells). Prior treatment of immune spleen cells with AKR anti-θ ascitic fluid and complement causes total abrogation of this in vivo activity. Fatal neurologic disease is induced only when donor and recipient share at least one set of H-2 antigenic specificities. Parent → F1 and F1 → parent combinations are as effective as syngeneic systems, but mice given allogeneic immune cells survive as long as controls. Differences at the M-locus in H-2 compatible mice do not inhibit effector activity. Homing of transferred lymphocytes to spleen is similar in syngeneic or allogeneic recipients, but only syngeneic immune cells cross the blood-cerebrospinal fluid (CSF) barrier ...
In a mouse colony in which lymphocytic choriomeningitis is endemic infection takes place either in utero or shortly after birth. Virus is discharged from infected mice with the nasal secretions and urine. In some mice the infection lasts for several months, and such carriers can transmit the disease to healthy mice by contact. The portal of entry appears to be the nasal mucosa rather than the gastrointestinal tract. Mice infected by contact show no definite symptoms while those infected in utero often do. The disease has persisted in the colony for at least 15 months without change in its character. Mouse stocks differ in their susceptibility to contact infection and the findings given in the paper could be reproduced only with a very susceptible stock. Wild mice (Mus musculus) can be infected by contact, although less easily than our white mice.. The source of the infection in the colony has not been determined. The fact that the serum of the caretaker neutralizes the virus indicates that he ...
We use simple mathematical models to examine the dynamics of primary and secondary cytotoxic T-lymphocyte (CTL) responses to viral infections. In particular, we are interested in conditions required to resolve the infection and to protect the host upon secondary challenge. While protection against reinfection is only effective in a restricted set of circumstances, we find that resolution of the primary infection requires persistence of CTL precursors (CTLp), as well as a fast rate of activation of the CTLp. Since these are commonly the defining characteristics of CTL memory, we propose that CTL memory may have evolved in order to clear the virus during primary challenge. W e show experimental data from lymphocytic choriomeningitis virus infection in mice, supporting our theory on CTL memory. We adapt our models to HIV and find that immune impairment during the primary phase of the infection may result in the failure to establish CTL memory which in turn leads to viral persistence. Based on our ...
PENSACOLA, Fla. - Top-ranked Armstrong Atlantic State advanced in penalty kicks, 6-5, past No. 19 Columbus State University on Friday afternooon, capturing the NCAA Division II Womens Soccer Southeast Region title and setting up a showdown with nationally-ranked No. 2 West Florida in the NCAA Quarterfinals on Sunday. The two teams played to a scoreless draw prior to PKs.. It was the first PK shootout win for Armstrong (19-1-2) in program history and keeps the Pirates drive towards a return trip to the Final Four alive. Columbus State (18-3-2) sees its season end on PKs in the NCAA Tournament for a third time in its history.. A tight defensive struggle between both teams generated just five shots between the two teams in the first half. The game opened up a little bit in the second half as each goalkeeper, Mary Mancin for Columbus State and Morgan Luckie for Armstrong, was called upon to make saves to preserve the shutout.. As time ran down in regulation, Columbus States Jackie Ellis had a ...
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Dr. David Armstrong, MD is a Colorectal Surgery Specialist in Alpharetta, GA. Dr. Armstrong has more experience with Colorectal Surgery and Transplant Surgery than other specialists in his area. He is affiliated with medical facilities such as Emory Decatur Hospital and Emory University Hospital Midtown. He is accepting new patients and has indicated that he accepts telehealth appointments. Be sure to call ahead with Dr. Armstrong to book an appointment.
Armstrong State University is now Georgia Southern University. For information about Fall 2018 and beyond, visit GeorgiaSouthern.edu. Current Armstrong students (spring and summer 2018) can continue to access information through Armstrong.edu. For details about the consolidation, visit consolidation.georgiasouthern.edu ...
In a series of emails to media organistions and cycling officials disgraced former Tour de France winner Floyd Landis yesterday admitted to systematic doping and implicated a number of other riders too including arguably cyclings biggest ever star, Lance Armstrong.. Amongst those emailed were the Wall Street Journal, it has been able to confirm to its satisfaction that the emails were genuinely from Landis - the newspaper has taken nearly three weeks to go public on the mails. That Landis doped is not a surprise, that he has finally admitted it is something of a bombshell given the loud and long campaign he waged, and which was waged by many on his behalf in the US media, to proclaim his innocence.. That particuar bombshell pales in to insignificance compared to what Landis has to say about his fellow American and erstwhile team mate, Lance Armstrong. However Armstrong, who has so far seemed to laugh off the accusations in allusions to them on his Twitter page, is not the only American rider ...
Armstrong State University is now Georgia Southern University. For information about Fall 2018 and beyond, visit GeorgiaSouthern.edu. Current Armstrong students (spring and summer 2018) can continue to access information through Armstrong.edu. For details about the consolidation, visit consolidation.georgiasouthern.edu ...
Looking for online definition of lymphocytic choriomeningitis (LCM) in the Medical Dictionary? lymphocytic choriomeningitis (LCM) explanation free. What is lymphocytic choriomeningitis (LCM)? Meaning of lymphocytic choriomeningitis (LCM) medical term. What does lymphocytic choriomeningitis (LCM) mean?
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To clarify the presence of lymphocytic choriomeningitis virus (LCMV) in Spain, we examined blood and tissue specimens from 866 small mammals. LCMV RNA was detected in 3 of 694 wood mice (Apodemus sylvaticus). Phylogenetic analyses suggest that the strains constitute a new evolutionary lineage. LCMV antibodies were detected in 4 of 10 rodent species tested.
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Henry Edward Armstrong FRS FRSE(Hon) (6 May 1848 - 13 July 1937) was an English chemist. Although Armstrong was active in many areas of scientific research, such as the chemistry of naphthalene derivatives, he is remembered today largely for his ideas and work on the teaching of science. Armstrongs acid is named for him. Armstrong was born the son of Richard Armstrong, a commission agent and importer, and Mary Ann Biddle. He lived most of his life in Lewisham, a suburb of London. After finishing school in 1864 at age 16, he spent a winter in Gibraltar, with a relative, for health reasons. In the spring of 1865, Armstrong returned to England and entered the Royal College of Chemistry in London, now the department of chemistry at Imperial College. Chemical training in those days was not lengthy, and at the age of 18 he was selected by Edward Frankland to assist in devising methods of determining organic impurities in sewage. Armstrong pursued further studies under Hermann Kolbe at Leipzig, ...
1 Bridget DWYER =Edward Palmer SEAMAN Marriage: 6 May 1842, Goulburn, , New South Wales, Australia 2 Mary A. SEAMAN 2 Sarah SEAMAN =Charles ARMSTRONG Marriage: 1864, Goulburn, , New South Wales, Australia 3 Mary A. ARMSTRONG =Joseph Edward HILLS Marriage: 1891, Crookwell, , New South Wales, Australia 3 Charles ARMSTRONG 3 Edward Palmer Seaman ARMSTRONG 3 Philip ARMSTRONG 3 Henry ARMSTRONG =Sarah MCPAUL Marriage: 1907, Goulburn, , New South Wales, Australia 3 Albina N. ARMSTRONG 3 Albert Edwin ARMSTRONG 3 Alice Adelaide ARMSTRONG 3 Oscar ARMSTRONG 3 Sydney ARMSTRONG 3 Florence Daisy G. ARMSTRONG 3 Beatrice Frances ARMSTRONG 2 Maria SEAMAN =Thomas Bradley WEEKS Marriage: 1867, Redbank, , NSW, AUS 3 Sidney E. WEEKS 3 Richard WEEKS =Ellen Emma G. BOYLE Marriage: 1891, Goulburn, , New South Wales, Australia 3 John Thomas WEEKS 3 Mary Anne WEEKS 3 Edward Seaman WEEKS =Mary May Elizabeth MCKENNA Marriage: 1900, Goulburn, , New South Wales, Australia 3 William Henry WEEKS 3 Robert Stanley WEEKS 3 Albert ...
Acute viral infections in humans and mice induce T lymphocyte responses which mediate viral clearance and result in the establishment of immunological memory. The course of an immune response to acute viral infection is associated with an immune deficiency in the lymphocyte compartment. This is usually characterized by the inability of lymphocytes to productively respond to mitogen or recall antigen. This thesis examined the acute lymphocytic choriomeningitis virus (LCMV) infection of the mouse and showed that T lymphocytes isolated from acutely LCMV-infected mice underwent activation-induced apoptosis upon signalling through the T-cell receptor (TcR)-CD3 complex. Kinetic studies demonstrated that this sensitivity to apoptosis directly correlated with the induction of immune deficiency, as measured by impaired proliferation in response to anti-CD3 antibody or to concanavalin A. Cell cycling in interleukin-2 (IL-2) alone stimulated proliferation of LCMV-induced T cells without inducing apoptosis, but
Prior immunity to a related or unrelated pathogen greatly influences the hosts immune response to a subsequent infection and can cause a dramatic difference in disease course, a phenomenon known as heterologous immunity. Heterologous immunity can influence protective immunity, immunopathology and/or immune deviation of cytokine-producing T cell subsets. Examples of heterologous immunity have been well documented in mouse models, as well as during human infections. For example, prior immunity to lymphocytic choriomeningitis virus (LCMV) provides partial protection against vaccinia virus (VV), as LCMV-immune mice show reduced VV titers and increased survival upon lethal dose VV infection. Heterologous protection against VV challenge, as a result of LCMV immunity, is mediated by LCMV-specific CD4 and CD8 T cells, as transfer of LCMV-specific memory T cells can mediate this protective effect in naïve mice. The recognition of a single TCR with more than one MHC-peptide complex is referred to as T cell
Studies were undertaken to characterize mechanisms for NK cell cytokine delivery in vivo. Conditions of systemic IFN-beta expression elicited by polyinosinic-polycytidylic acid (poly(I:C)) treatment or IFN-alpha beta production during lymphocytic choriomeningitis virus or murine cytomegalovirus infections resulted in profound splenic histologic changes, with relocalization of nucleated cells from red to white pulp regions. Cell-trafficking experiments, with fluorescently labeled populations, showed that poly(I:C) induced T/B cell-dependent leukocyte migration into white pulp regions. Splenic leukocytes prepared from severe combined immunodeficient (SCID) and bone marrow cells prepared from SCID or normal C57BL/6 mice revealed a unique poly(I:C)-induced accumulation of non-T/non-B cells along splenic red and white pulp region borders characteristic of marginal zones. Lymphocytic choriomeningitis virus and murine cytomegalovirus infections also induced this trafficking pattern. Ab treatments of ...
PubMed journal article: Lymphoma associated with an epizootic of lymphocytic choriomeningitis in Syrian hamsters (Mesocricetus auratus). Download Prime PubMed App to iPhone, iPad, or Android
Michael B.A. Oldstone is a Professor in the Department of Immunology & Microbial Science at The Scripps Research Institute where he heads the Viral-Immunobiology Laboratory. He focuses on viral-host interactions, especially how viruses cause disease. His work studying the prototype arenavirus lymphocytic choriomeningitis virus dissected the biologic and molecular parameters of how virus infection leads to the submission or the enhancement of the immune response. Submission or subversion leads to viral persistence and enhancement to autoimmunity and immunopathology. Using experimental animal model systems, his findings, especially with virus-specific cytotoxic T cells, with molecules that negatively regulate such cytotoxic T cells and with formation and consequences of viral antibody immune complexes, have been extended to infectious diseases of humans. These paradigm observations, coupled with his work on viral variants and dendritic cell interactions have led to his election to the National ...
|jats:p|The highly sensitive quantitation of virus-specific CD8+ T cells using major histocompatibility complex-peptide tetramer assays has revealed higher levels of cytotoxic T lymphocytes (CTLs) in acute and chronic virus infections than were recognized previously. However, studies in lymphocytic choriomeningitis virus infection have shown that tetramer assays may include measurement of a substantial number of tetramer-binding cells that are functionally inert. Such phenotypically silent CTLs, which lack cytolytic function and do not produce interferon (IFN)-γ, have been hypothesized to explain the persistence of virus in the face of a quantitatively large immune response, particularly when CD4 help is impaired. In this study, we examined the role of functionally inert CTLs in chronic HIV infection. Subjects studied included children and adults (n = 42) whose viral loads ranged from <50 to >100,000 RNA copies/ml plasma. Tetramer assays were compared with three functional assays: enzyme
The Pathology, Diagnosis and Treatment of Perforated Gastric Ulcer by George E. Armstrong by G. E. (George E. )., Armstrong available in Trade Paperback on Powells.com, also read synopsis and reviews.This is a pre-1923 historical reproduction that was curated for quality. Quality assurance was...
Next, Armstrong announces a trip to his hometown of New Orleans, which, again, inspires a roar of approval from a crowd that sounds like they were ready to cheer just about anything. The tune is The Buckets Got A Hole In It and Armstrong announces it as being written during the days of rushing the cans. Thats a reference to the kids whose job it was to bring beer to the bordellos. Jelly Roll Morton, on his seminal 1939 recording of Mamies Blues, opens with a spoken introduction where he says, This is the first blues I no doubt heard in my life. Mamie Desdunes, this is her favorite blues. She hardly could play anything else more, but she really could play this number. Of course, to get in on it, to try to learn it, I made myself the, the can rusher. Armstrong usually announced The Buckets Got A Hole In It as being played by Joe Oliver in New Orleans and it was recorded in the 1920s by the likes of Lil Johnson and Washboard Sam, with composer credit going to Clarence Williams. ...
Portneuf was built using a fast track construction schedule, which meant the Ascension Group worked with an accelerated timeline for project delivery. But, finding interior finish colors that are the right colors and that work well together can take a lot of time. The Armstrong CONTINUUM system assists architects and designers working on fast track projects by allowing designers to quickly mix and match flooring choices to achieve a naturally integrated look. It also simplifies the design process because the colors and designs are coordinated within product lines and cross-coordinated with other Armstrong flooring lines. Three floor products- ColorArt Medintone Sheet, LinoArt MARMORETTE Linoleum and Premium EXCELON Stonetex VCT- feature Armstrong CONTINUUM and are used throughout the Portneuf project.. Designers chose seven coordinating ColorArt MEDINTONE Sheet vinyl colors for the halls and patient rooms. ColorArt MEDINTONE Sheet has proven itself to the hospital owners and our firm to be a ...
Back when he announced his new foray into the pro cycling world, and perhaps the Tour de France, Lance Armstrong stated he was developing his own private doping label. Apparently the 7-time Tour winner is too busy to follow through with the details. Lance Armstrong has stated that his personal drug-testing program will be in place by the time of his first race at the Tour Down Under in January - but no sooner. When seven-times Tour de France winner Armstrong announced his comeback to professional cycling in September, he also announced that he would be part of a rigorous...
Initiation of antiviral and anti-tumour T cell responses is probably achieved mainly by dendritic cells (DC) transporting antigen from the periphery into organised lymphoid tissues. To develop T cell vaccines it is, therefore, important to understand the accessibility of the antigen to DC in vivo and whether DC are activated by vaccination. Here we have evaluated the immunogenicity of a liposomal vaccine formulation with antigenic peptides derived from the glycoprotein of the lymphocytic choriomeningitis virus. Liposome-encapsulated peptides were highly immunogenic when administered intradermally and elicited protective antiviral immunity. After intradermal injection, liposomes formed antigen depots which facilitated long-lasting in vivo antigen loading of dendritic cells almost exclusively in the local draining lymph nodes. The immunogenicity of the liposomal peptide vaccine was further enhanced by incorporation of immunostimulatory oligonucleotides leading to activation of DC. This optimised ...
CD8 T cells are recognized key players in control of persistent virus infections, but increasing evidence suggests that assistance from other immune mediators is also needed. Here, we investigated whether specific antibody responses contribute to control of lymphocytic choriomeningitis virus (LCMV), a prototypic mouse model of systemic persistent infection. Mice expressing transgenic B cell receptors of LCMV-unrelated specificity, and mice unable to produce soluble immunoglobulin M (IgM) exhibited protracted viremia or failed to resolve LCMV. Virus control depended on immunoglobulin class switch, but neither on complement cascades nor on Fc receptor gamma chain or Fc gamma receptor IIB. Cessation of viremia concurred with the emergence of viral envelope-specific antibodies, rather than with neutralizing serum activity, and even early nonneutralizing IgM impeded viral persistence. This important role for virus-specific antibodies may be similarly underappreciated in other primarily T cell-controlled
Generation of reactive oxygen intermediates (ROI) following antigen receptor ligation is critical to promote cellular responses. However, the effect of antioxidant treatment on humoral immunity during a viral infection was unknown. Mice were infected with lymphocytic choriomeningitis virus (LCMV) and treated with Mn(III)tetrakis(4-benzoic acid)porphyrin chloride (MnTBAP), a superoxide dismutase mimetic, from days 0 to 8 postinfection. On day 8, at the peak of the splenic response in vehicle-treated mice, virus-specific IgM and IgG antibody-secreting cells (ASC) were decreased 22- and 457-fold in MnTBAP-treated animals. By day 38, LCMV-specific IgG ASC were decreased 5-fold in the bone marrow of drug-treated mice, and virus-specific antibodies were of lower affinity. Interestingly, antioxidant treatment had no effect on the number of LCMV-specific IgG memory B cells. In addition to decreases in ASC, MnTBAP treatment decreased the number of functional virus-specific CD4+ T cells. The decreased numbers of
More damningly, it paints Armstrong as the driving force behind a sophisticated, worldwide doping ring involving riders, coaches, and trainers.. At this point, there is only one argument left for Armstrong to make: everyone was doing it. Specifically, 20 of 21 top-3 finishers from 1999 to 2005 were doing it, and 36 of 45 top-3 finishers from 1996 to 2010 were doing it. Take a look at this paragraph (which was slipped into the introduction of the report without much comment) in the USADA report:. 20 of the 20-one podium finishers in the Tour de France from 1999 through 2005 have been directly tied to likely doping through admissions, sanctions, public investigations or exceeding the UCI hematocrit threshold. Of the 40-five podium finishes during the time period between 1996 and 2010, 30-six were by riders similarly tainted by doping.. So in a 15-year period, there were only 9 riders who managed to succeed without cheating, according to the USADA.. Its fair to say that in that period, doping was ...
The Ethel Louise Armstrong Foundation, Inc. (ELA) is pleased to announce that the 2003 ELA Scholarship application is now available in the scholarship section of the ELA website at www.ela.org. The ELA Scholarship provides financial assistance to women with physical disabilities who are enrolled in a college or university graduate program in the United States. ELA Scholarship awards are based on merit and are given in an objective and nondiscriminatory basis. Each applicant is required to submit an application packet including an application form, a college transcript, two letters of academic recommendation, a medical verification form and an essay outlining how they will Change the Face of Disability on the Planet. Scholarships range from $1,000 to $2,000 per year. The application deadline is June 1st, 2003. The vision of the Ethel Louise Armstrong (ELA) Foundation, Inc. is to Change the Face of Disability on the Planet. Our mission is to promote, through grants and scholarships, the ...
Den Neil Armstrong koum 1930 op enger Farm, zéng Kilometer vu Wapakoneta am Ohio ewech, op dWelt. Säi Papp Stephen war staatleche Rechnungspréifer, dMamm Viola war Hausfra. Ee vu sengen Urgrousspäpp huet vu Ladbergen (Nordrhein-Westfalen) gestaamt. Zanter senger Jugend, wou hie Scout bei de Boy Scouts of America war, hat hie sech fir dFligerei interesséiert. Hien huet Modellfliger gebastelt an hat scho mat 17 Joer eng Pilotelizenz. No der High School krut hien 1947 vun der US-Marine e Stipendium fir op der Purdue University e Studium fir Fligeringenieur ze maachen. No dräi Semester huet dNavy hien am Januar 1949 agezunn an a Florida geschéckt. Zu Pensacola gouf hien zum Kampfpilot ausgebilt. Vun 1950 un war den Armstrong am Koreakrich dobäi. Hien huet zum Fighter Squadron 51 (VF-51) gehéiert a war mat 20 Joer de jéngste Member vun der Eenheet, déi um Fligerdréier USS Essex stationéiert war. Vun do ass hie mat engem F9F „Panther am Ganzen 78 Asätz geflunn. Den 3. September ...
Stephen eta Viola Armstrongen semea, haurtzaroan hainbat komunitatetan bizi izan zen, izan ere, bere aita Ohio estatuko Bobsville hiriko auditorea zen; honela, Armstrong gaztea hainbat hiritan hazi zen: Warren, Jefferson, Ravenna, St. Marys eta Upper Sandusky. Azkenik, bere familiak Wapakonetan behin betiko bizitzea erabaki zuen. Hegan egiteko interesa bi urte besterik ez zuela piztu zitzaion Armstrongi, aitak Ohioko Nazio Aire-lasterketetara eraman zuenean. Bere interesa sei urte zituela areagotu zen, aireplano batean bere lehenengo buelta egin zuenean; berau hiru motorreko Ford bat zen, edo, era informalean deitzen zioten bezala, Latorrizko Antzara. Harrezkero, hegazkinek liluratu egin zuten. Hamabost urte zituelarik, Armstrong Wapakoneta hiriaren iparraldeko aireportu batean hegan egiteko ikastaro bat egiten hasi zen, diru pixka bat irabazteko eta eskolak ordaintzeko herrian zein aireportuan bertan lan batzuk eginez. Hamasei urterako, gidatzeko azterketa gainditu baino lehen, pilotu-ikasle ...
Inherited defects of granule-dependent cytotoxicity led to the life-threatening immune disorder hemophagocytic lymphohistiocytosis (HLH), characterized by uncontrolled CD8 T-cell and macrophage activation. In a cohort of HLH patients with genetic abnormalities expected to result in the complete absence of perforin, Rab27a, or syntaxin-11, we found that disease severity as determined by age at HLH onset differed significantly, with a severity gradient from perforin (early onset) | Rab27a | syntaxin-11 (late onset). In parallel, we have generated a syntaxin-11-deficient (Stx11(-/-)) murine model that faithfully reproduced the manifestations of HLH after lymphocytic choriomeningitis virus (LCMV) infection. Stx11(-/-) murine lymphocytes exhibited a degranulation defect that could be rescued by expression of human syntaxin-11 but not expression of a C-terminal-truncated mutant. Comparison of the characteristics of LCMV infection-induced HLH in the murine counterparts of the 3 human conditions revealed a
Rodents with hanta virus, lymphocytic choriomeningitis virus (LCMV) and leptospirosis usually do not exhibit signs of disease. The disease agents are typically shed in the urine of infected animals and people acquire the infection by inhalation, oral ingestion and direct contact with contaminated urine or feces. These are occasionally transmitted from bite wounds and Leptospira can infect people through abraded skin. These diseases often initially appear as a mild flu-like illness in people but may progress to severe disease. LCMV infection is considered hazardous to the unborn fetus. Please refer to the WSU Hantavirus guidelines (http://www.iacuc.wsu.edu/documents/forms/pdf/Zoonoses_19.pdf) if working with wild rodents or in rodent-infested areas and buildings. Salmonellosis and campylobacterosis are acquired by contact and accidental ingestion of fecal material from infected rodents. Animals infected with these diseases may have diarrhea but some may show no symptoms of disease. Any animal ...
|jats:p|For viruses to establish persistent infections in their hosts, they must possess some mechanism for evading clearance by the immune system. When inoculated into adult immunocompetent mice, wild-type lymphocytic choriomeningitis virus (LCMV ARM) induces a CD8(+)-mediated cytotoxic T lymphocyte (CTL) response that clears the infection within 7-14 d (CTL+ [P-]). By contrast, variant viruses isolated from lymphoid tissues of persistently infected mice fail to induce a CTL response and are thus able to establish a persistent infection in adult mice (CTL- [P+]). This report compares the interaction of CTL+ (P-) and CTL- (P+) viruses with cells of the immune system. Both types of virus initially bind to 2-4% of CD4+ and CD8+ T lymphocytes and replicate within cells of both subsets. The replication of CTL- (P+) and CTL+ (P-) viruses in lymphocytes in vivo is similar for the first 5 d after initiating infection. Thereafter, in mice infected with CTL- (P+) variants, lymphocytes retain viral genetic
Structural characterization of peptides restricted by major histocompatibility complex (MHC) class I molecules has identified residues critical for MHC class I binding and for T-cell receptor recognition. For example, optimal peptides fitting into the murine MHC class I Db groove are 9 to 11 amino acids long and require as MHC anchor residues an Asn (N) at position 5 and also either a hydrophobic residue, a Met (M) or a Cys (C), at the carboxy terminus. The three known Db-restricted peptides of lymphocytic choriomeningitis virus (LCMV) are glycoproteins GP1 (amino acids [aa] 33 KAVYNFATC), GP2 (aa 276 SGVENPGGYCL), and nucleoprotein NP (aa 396 FQPQNGQFI). In addition to these two GP and one NP peptides, computer search revealed 11 other GP peptide sequences and 20 additional NP sequences that contained the Db binding motif. By Db competitive binding analysis, only two of these 11 GP peptides and 1 of these 20 NP peptides bound to the MHC Db molecule with an affinity equivalent to the measured ...
Beura LK, Hamilton SE, Bi K, Schenkel JM, Odumade OA, Casey KA, Thompson EA, Fraser-- KA, Rosato PC, Filali-Mouhim A, Sekaly RP, Jenkins MK, Vezys V, Haining WN, Jameson SC, Masopust D. --Normalizing the environment recapitulates adult human immune traits in laboratory mice. 2016. Nature. 532:512-6.. Steinert EM, Schenkel JM, Fraser KA, Beura LK, Manlove LS, Igyarto BZ, Southern P, Masopust D. Quantifying memory CD8 T cells reveals regionalization of immunosurveillance. 2015. Cell. 161:737-49.. Beura LK, Anderson KG, Schenkel JM, Locquiao JJ, Fraser KA, Vezys V, Pepper M, Masopust D. Lymphocytic choriomeningitis virus persistence promotes effector-like memory differentiation and enhances mucosal T cell distribution. 2015. 97:217-25. J Leuk Bio.. Schenkel JM, Masopust D. Tissue-resident memory CD8 T cells. 2014. Immunity. 41:886-897.. Schenkel JM, Fraser KA, Beura LK, Vezys V, Masopust D. Resident memory CD8 T cells trigger a rapid organ defense response through cytokines. 2014. Science. ...
SH2D1A, which encodes signaling lymphocyte activation molecule (SLAM)-associated protein (SAP), is altered in patients with X-linked lymphoproliferative disease (XLP), a primary immunodeficiency. SAP-deficient mice infected with lymphocytic choriomeningitis virus had greatly increased numbers of CD8+ and CD4+ interferon-gamma-producing spleen and liver cells compared to wild-type mice. The immune responses of SAP-deficient mice to infection with Leishmania major together with in vitro studies showed that activated SAP-deficient T cells had an impaired ability to differentiate into T helper 2 cells. The aberrant immune responses in SAP-deficient mice show that SAP controls several distinct key T cell signal transduction pathways, which explains in part the complexity of the XLP phenotypes.
Soon after Lance Armstrong announced that he was stepping down as chairman of the cancer foundation that bears his name and Nike finally announced it had severed ties with the Texan, the Twitter masses took to their scrolling feeds, exhorting their opinions on the two massive announcements in Armstrongs world. Most commenters were applauding the decision, and interestingly enough, many began to blame Nike for the hero worship of Armstrong and the corporations part in what many are tagging the greatest sporting fraud in history.. From 1999 to just a mere month ago, Armstrong (and his minions) threatened, bullied, sued, intimidated, and bought off the media, his sponsors, his teammates, and allegedly members of national federations and the UCI to ensure their silence. Armstrong was the toast of Hollywood. He was the darling of the political set. It was a fairy tale story.. Last night during an appearance on Bicycle Radio about the Armstrong experience, one of the co-hosts asked my thoughts on ...
Soon after Lance Armstrong announced that he was stepping down as chairman of the cancer foundation that bears his name and Nike finally announced it had severed ties with the Texan, the Twitter masses took to their scrolling feeds, exhorting their opinions on the two massive announcements in Armstrongs world. Most commenters were applauding the decision, and interestingly enough, many began to blame Nike for the hero worship of Armstrong and the corporations part in what many are tagging the greatest sporting fraud in history.. From 1999 to just a mere month ago, Armstrong (and his minions) threatened, bullied, sued, intimidated, and bought off the media, his sponsors, his teammates, and allegedly members of national federations and the UCI to ensure their silence. Armstrong was the toast of Hollywood. He was the darling of the political set. It was a fairy tale story.. Last night during an appearance on Bicycle Radio about the Armstrong experience, one of the co-hosts asked my thoughts on ...
Mathur, G., Yadav, K., Ford, B., Schafer, I., Basavaraju, S., Knust, B., Shieh, W., Hill, S., Locke, G., Quinlisk, P., Brown, S., Gibbons, A., Cannon, D., Kuehnert, M., Nichol, S., Rollin, P., Ströher, U. & Miller, R. (2017). High clinical suspicion of donor-derived disease leads to timely recognition and early intervention to treat solid organ transplant-transmitted lymphocytic choriomeningitis virus. Transplant Infectious Diseases, 4(19). PMID: 28423464.. Ford, B. A. (2017). mecC-MRSA: Hiding in Plain Sight. Journal of clinical microbiology. PMID: 29118173.. Dunseth, C. D., Ford, B. A. & Krasowski, M. D. (2017). Traditional versus reverse syphilis algorithms: A comparison at a large academic medical center. Practical Laboratory Medicine, 8(2017), 52-59. DOI: 10/1016/j.plabm.2017.04.007.. Marra, A. R., Edmond, M. B., Ford, B. A., Herwaldt, L. A., Algwizani, A. R. & Diekema, D. J. (2016). Failure of Risk-Adjustment by Test Method for C. difficile Laboratory-Identified Event Reporting. Infection ...
Case-patient 2, a 39-year-old man, sought treatment in May 2010 with headache, nausea, vomiting, increased perspiration, and a temperature of 37.5°C. Aseptic meningitis was suspected, and CSF and serum samples were collected. CSF analysis demonstrated 1,715 leukocytes/mm3 (95% mononuclear cells), normal glucose level (68 mg/dL), and elevated protein levels (240 mg/dL). Results of a cranial computed tomographic scan were normal. Further virologic investigation detected LCMV RNA in the CSF and an IgG titer of 640 by IFA and IgM antibodies against LCMV in the serum sample. Serum amount was insufficient to conduct IgM titration and Western blot assay. No CSF sample was available to attempt viral isolation. The sequence of a 194-nt PCR product (nucleocapsid protein gene) obtained was most closely related to sequences of the lineage I. Sequence homology among the LCMV amplicon from case-patient 2 and lineage I strains was ,87% versus 77%-79% sequence homology among case-patient 2 and strains from ...
There are data to support both coinhibitory and costimulatory functions for CD160. CD160 can promote NK cell cytotoxicity and IFN-γ production in vitro and in vivo (4, 10, 13, 29). In vitro binding of HVEM to CD160 on human CD4+ T cells delivered an inhibitory signal to the T cell (5), whereas anti-CD160 mAb enhanced the function of exhausted CD8+ T cells from LCMV clone 13-infected mice in vitro (15). Less is known about the function of CD160 on T cells in vivo. Recent reports indicating that CD160 may regulate immune responses during enteric infections (8, 26) led us to investigate CD160 expression and function in the gut microenvironment as well as during acute and chronic LCMV infection. Our studies reveal that CD160 promotes CD8+ T cell effector functions during oral infection with L. monocytogenes and is critical for optimal clearance of this acute bacterial infection.. We identified several factors that regulate CD160 expression in IELs in the intestinal microenvironment. We determined ...
Our hypothesis was that if we could artificially induce conditions like those produced by the Armstrong strain, it would help the immune system clear an infection by clone 13, says Altman.. His team turned to an experimental drug called FTY720, which prevents white blood cells from leaving lymph nodes.. FTY720, also known as fingolimod, desensitizes white blood cells so they cant respond to the chemical messenger sphingosine-1-phosphate (S1P). S1P also influences heart rate and smooth muscle contraction in the airways.. Scientists had previously thought of FTY720 as something that suppresses the immune system, Altman says. While not approved for sale by the FDA, doctors have tested it for the treatment of multiple sclerosis and preventing kidney transplant rejection.. Even if mice have a stable chronic LCMV clone 13 infection, treatment with FTY720 can still improve their immune response against LCMV enough to have them rid it from their systems, the authors found.. FTY720 appears to prevent ...
Court documents show that an Ohio hospital paid the estate of astronaut Neil Armstrong $6 million to settle allegations that post-surgical complications led to his 2012 death.
Neil Armstrong changed the world by becoming the first man in history to successfully pilot a spacecraft to land safely on the lunar surface and then walk on the moon. This not only was a high point...
Professor Armstrong leads a research group concerned with mechanistic and exploratory Bioinorganic Chemistry. The principal aims are to understand the chemical reactions carried out by the active sites of metalloproteins (a well-known example would be the Fe-porphyrin group as occurs in haemoglobin) and to elucidate how long-range electron transfer in complex protein systems is coupled to catalysis and ion/proton transfer.. Subjects range from the properties of unstable Fe-S clusters and Fe(IV)=O (to most chemists an unusual oxidation state) to medically important respiratory chain electron transport enzymes such as succinate dehydrogenase. To study these systems, his group uses a range of kinetic techniques, and is currently developing a powerful new method in which the chemistry of metalloprotein active sites is probed electrochemically while they are absorbed on a carbon electrode surface ...
The CHRocodile MPS optical sensor is a new multi-channel system that provides outstanding value per channel of operation. With up to 24 independent channels a mixture of single point distance and thickness measurements can be acquired at up to 108,000 measurements per second. When used in conjunction with the new MPS 10 optical probe a line of 10 points is able to measure either distance or thickness from ten positions simultaneously. The CHRocodile MPS has been designed for industrial environments - it is housed in a 19″ rack and is robust and maintenance free. As well as Ethernet, USB and RS422 interfaces it also has 5 encoder inputs, trigger input, and sync-output. It is perfectly suited for challenging measuring tasks, such as non-contact measurements of container glass where out of specification items can be found more reliably saving money and time by avoiding unnecessary rejects. For more details please download the datasheet by clicking the link CHRocodile MPS. Optical probe MPS 10 ...
The CLS chromatic confocal line scan sensor system is available in two configurations - linear and with a 90° fold. Three line widths are available of 1, 2 and 5mm length providing a data-point pitch of 5, 10 and 25µm along the line. The three line-widths offer measuring ranges of 0.2, 1 and 4mm and resolutions of 30, 150 and 300nm respectively. All configurations can operate at data collection rates of up to 2KHz.. Ths CLS chromatic confocal system will soon be available for demonstration - if you have an application that you feel would benefit from the linescan performance dont wait - just call us on +44 (0) 1604 654220 to talk to one of our specialists or CLICK HERE. For more information please download the Precitec Optronik CLS chromatic confocal brochure.. Wondering about chromatic confocal measurement? Click this link to have it explained Chromatic Confocal Measurement principles, ...
While Ill always remember him for the part he played in a ridiculous local commercial when he was a member of the Pittsburgh Penguins, Colby Armstrong is also known for his rugged style mixed with a touch of half-decent scoring ability.
Armstrong International offers a large range of liquid drainers which are suitable for automatically draining water or consensate from air of gas systems.. These are needed due to the fact that if water or condensate builds up in these systems, it can cause severe damage to them and to the valves due to water hammer or corrosion. This is because warer / condensate acts as an insulator to heat.. The firms liquid trainer can be used on drainers from any industry, however they are particularly used in the oil and gas sectors.. Typical applications include draining gas condensate from filters / separators and water from compressed air receivers.. ...
EPO can be detect 2-4 days after injection, and therefore, if the samples were taken during this time interval and freezed for long term storage (-80C), these samples would be just as good as the day they were taken.. I am a biochemist by training, and for people to argue that these samples are too old is ridiculous. Posted by: Gregory Timbledon at Aug 23, 2005 9:09:21 PM. Well, like the president of the doping lab says; the EPO is either broken down...or it isnt. If it is, it will not be detected. If synthetic EPO is discovered, there is no other way to explain than that the rider must have doped.. On the comment by Sara Collison on cancer patients receiving EPO; Armstrong himself has said in his book Its not about the bike (that title has a different ring to it now doesnt it? Hehehe) that he received EPO when his hematocrit level was very low. But this was in 1996 of 1997. Although that might have been in his hairs later on, it will definitely not have been in his urine during the ...
Find, compare and select ballet dancing classes & lessons for kids in Armstrong Beach (4737). Read reviews of kids activities and events. ActiveActivities - Australias leading online marketplace for kids activities.
"Morphological and Cytochemical Studies on Lymphocytic Choriomeningitis Virus". Journal of Virology. 2 (12): 1465-1478. doi: ... He was among the first "to recognize the role of the immune response in the pathogenesis of murine lymphocytic choriomeningitis ... over 1300 citations) Hartley, J. W.; Wolford, N. K.; Old, L. J.; Rowe, W. P. (1977). "A new class of murine leukemia virus ... Hoggan, M. D.; Blacklow, N. R.; Rowe, W. P. (1966). "Studies of small DNA viruses found in various adenovirus preparations: ...
"Monoclonal antibodies to lymphocytic choriomeningitis and pichinde viruses: Generation, characterization, and cross-reactivity ... "Monoclonal antibodies to lymphocytic choriomeningitis virus react with pathogenic arenaviruses". Nature. 288 (5790): 486-487. ... in Nigeria in 2018 Impact of yellow fever on the developing world Monoclonal antibodies to lymphocytic choriomeningitis virus ... WHO Working Group on Geographic Risk for Yellow Monoclonal antibodies to lymphocytic choriomeningitis and pichinde viruses: ...
Old World arenaviruses include lymphocytic choriomeningitis virus and Lassa virus. New world arena viruses are further broken ... A notable event in the timeline of this virus' scientific knowledge was the unexplained disappearance of a vial of the virus at ... "Virus vial missing from Galveston lab". ETX News. 24 March 2013. Archived from the original on 30 March 2013.. ... On the biosafety level scale of one to four, with four causing the most risk, the viruses causing hemorrhagic fevers have been ...
... influenza virus, lymphocytic choriomeningitis virus, polyomavirus, hepatitis delta virus, and hepatitis C virus. Although ADAR1 ... "A-to-G hypermutation in the genome of lymphocytic choriomeningitis virus". Journal of Virology. 81 (2): 457-64. doi:10.1128/jvi ... This seems to be true for the HCV replicon, Lymphocytic choriomeningitis LCMV, and polyomavirus ADAR1 is known to be proviral ... ADAR1's A to I editing has been found in many viruses including measles virus, ...
"Identification of alpha-dystroglycan as a receptor for lymphocytic choriomeningitis virus and Lassa fever virus". Science. 282 ... "Lassa virus RefSeq Genome". Cornu TI, de la Torre JC (October 2001). "RING finger Z protein of lymphocytic choriomeningitis ... It shares this receptor with the prototypic Old World arenavirus lymphocytic choriomeningitis virus. Receptor recognition ... Lassa and lymphocytic choriomeningitis virus instead use an endocytotic pathway independent of clathrin, caveolin, dynamin and ...
Jamieson DJ, Kourtis AP, Bell M, Rasmussen SA (June 2006). "Lymphocytic choriomeningitis virus: an emerging obstetric pathogen ... Bonthius, DJ (September 2012). "Lymphocytic choriomeningitis virus: an underrecognized cause of neurologic disease in the fetus ... "Interim guidance for minimizing risk for human lymphocytic choriomeningitis virus infection associated with rodents". MMWR. ... "Lymphocytic Choriomeningitis" (PDF). Iowa State University Center for Food Security and Public Health. March 2010. Verhaegh EM ...
"Identification of alpha-dystroglycan as a receptor for lymphocytic choriomeningitis virus and Lassa fever virus". Science. 282 ...
He coined the name Lymphocytic choriomeningitis in 1934 after isolating the hitherto completely unknown virus. He discovered in ... which he named lymphocytic choriomeningitis (LCM) and (b) to his successful work in polio research and polio prevention. In ... which led him to name the disease lymphocytic choriomeningitis. While still doing research work on LCM in 1939, Armstrong was ... which he had labelled lymphocytic choriomeningitis, "La Maladie d'Armstrong". 1938-9 Appointment to General Advisory Committee ...
"High prevalence of antibodies to lymphocytic choriomeningitis virus in a murine typhus endemic region in Croatia". Journal of ... The island is one of the biggest described endemic places of origin of house-mouse-borne lymphocytic choriomeningitis in the ...
Lìjiāng virus (LIJV) Loei River virus (LORV) Lujo virus(LUJV) Luna virus (LUAV) Lunk virus (LNKV) Lymphocytic choriomeningitis ... Alxa virus (ALXV) Dandenong virus (DANV) Gairo virus (GAIV) Gbagroube virus Ippy virus (IPPYV) Kodoko virus (KODV) Lassa virus ... Merino Walk virus (MRWV) Menekre virus Minu virus Mobala virus (MOBV) Morogoro virus (MORV) Mopeia virus (MOPV) Ryukyu virus ( ... Flexal virus (FLEV) Paraná virus (PRAV) Pichindé virus (PICHV) Pirital virus (PIRV) Clade B Amaparí virus (AMAV) Aporé virus ( ...
For instance, West Nile virus, mumps, measles, herpes simplex types I and II, varicella and lymphocytic choriomeningitis (LCM) ... Herpes simplex virus, varicella zoster virus and cytomegalovirus have a specific antiviral therapy. For herpes the treatment of ... common stomach viruses).[3][4][5] However, other viruses can also cause viral meningitis. ... Viruses are the most common cause of aseptic meningitis.[2] Most cases of viral meningitis are caused by enteroviruses ( ...
... generation and maintenance of functional virus-specific CD8+ T cells in mice infected with lymphocytic choriomeningitis virus ... March 2010). "Transient CD86 expression on hepatitis C virus-specific CD8+ T cells in acute infection is linked to sufficient ... May 2010). "Large-scale candidate gene analysis of spontaneous clearance of hepatitis C virus". The Journal of Infectious ... June 2010). "Interleukin-9 polymorphism in infants with respiratory syncytial virus infection: an opposite effect in boys and ...
RNA virus. MeV Subacute sclerosing panencephalitis. LCV Lymphocytic choriomeningitis. Arbovirus encephalitis. Orthomyxoviridae ... DNA virus. HBV (B). RNA virus. CBV. HAV (A). HCV (C). HDV (D). HEV (E). HGV (G). ... Human parainfluenza virus infection Human parainfluenza viruses (HPIV) Hymenolepiasis Hymenolepis nana and Hymenolepis diminuta ... DNA virus. HBV Hepatocellular carcinoma. HPV Cervical cancer. Anal cancer. Penile cancer. Vulvar cancer. Vaginal cancer. ...
Critical for the Anti-Interferon Activity of the Nucleoprotein of the Prototypic Arenavirus Lymphocytic Choriomeningitis Virus ... "Vaccination of Pigs against Swine Influenza Viruses by Using an NS1-Truncated Modified Live-Virus Vaccine". Journal of Virology ... "An enzymatic virus-like particle assay for sensitive detection of virus entry". Journal of Virological Methods. 163 (2): 336- ... "Inhibition of Interferon-Mediated Antiviral Responses by Influenza a Viruses and Other Negative-Strand RNA Viruses". Virology. ...
IL-21 (or IL-21R) knock-out mice infected with chronic LCMV (lymphocytic choriomeningitis virus) were not able to overcome ... In addition, IL-21 producing virus specific CD8 T cells were also preferentially found in HIV controllers. These data and the ... and proliferation in CD8 T cells of patients with human immunodeficiency virus-1 (HIV)". Blood. 109 (9): 3873-80. doi:10.1182/ ... rare individuals who don't progress to AIDS by controlling the virus replication without treatment) are able to produce ...
... cowpox virus and adenovirus serotype 5. Evidence has shown that the clearance of lymphocytic choriomeningitis virus by marginal ... Furthermore, both types of marginal zone macrophages in the clearance and degradation of viruses e.g. ...
Human immunodeficiency virus (HIV); causes AIDS La Crosse virus Lymphocytic choriomeningitis virus (LCMV) Measles Mumps St. ... such as West Nile virus, mumps, measles, herpes simplex types I and II, varicella and lymphocytic choriomeningitis (LCM) virus ... Lymphocytic choriomeningitis virus (LCMV) can be differentiated from the common presenting meningeal symptoms by the appearance ... Louis encephalitis virus West Nile virus Play media Viral Meningitis is mostly caused by an infectious agent that has colonized ...
... lymphocytic choriomeningitis virus infection, and herpes simplex virus. Some tests should be repeated up to 6 months later as ... "Zika Virus". CDC. 5 November 2014. Retrieved 20 June 2019. "Symptoms, Diagnosis, & Treatment of Zika Virus". Zika Virus Home. ... Zika virus has been isolated from semen samples, with one person having 100,000 times more virus in semen than blood or urine, ... Zika virus is a mosquito-borne flavivirus closely related to the dengue and yellow fever viruses. While mosquitoes are the ...
... on the role of lymphocytic choriomeningitis virus in prenatal human pathology, in 1980, at the age of 71. In 1993, Šeinbergas ...
Baldridge JR, Buchmeier MJ (1992). "Mechanisms of antibody-mediated protection against lymphocytic choriomeningitis virus ... Stram Y, Kuzntzova L. (2006). "Inhibition of viruses by RNA interference". Virus Genes. 32 (3): 299-306. PMID 16732482.. ... Kebanyakan vaksin virus berasal dari virus yang dilemahkan, sedangkan banyak vaksin bakteri berasal dari komponen aseluler dari ... Virus dikonfirmasi sebagai patogen manusia pada 1901 dengan penemuan virus demam kuning oleh Walter Reed.[7] ...
Technology in Zurich showed that LAG3 was an exhaustion marker for CD8+ T cells specific for Lymphocytic choriomeningitis virus ...
... lassa virus MeSH B04.820.057.070.100.550 - lymphocytic choriomeningitis virus MeSH B04.820.057.070.800 - arenaviruses, new ... lassa virus MeSH B04.909.777.080.070.100.550 - lymphocytic choriomeningitis virus MeSH B04.909.777.080.070.800 - arenaviruses, ... yellow fever virus MeSH B04.820.250.400 - gb virus a MeSH B04.820.250.405 - gb virus b MeSH B04.820.250.410 - GB virus C MeSH ... yellow fever virus MeSH B04.909.777.310.400 - gb virus a MeSH B04.909.777.310.405 - gb virus b MeSH B04.909.777.310.410 - GB ...
... performing research on vaccines and viruses, including pseudorabies virus and lymphocytic choriomeningitis virus (LCM). During ... immunity in white mice and its relationship to Lymphocytic choriomeningitis (LCM), which had long been a research interest of ... Also in 1953, he published another paper for the Navy with Worth I. Capps on the foot-and-mouth disease virus and methods for ... In 1944, Blome sent Traub to pick up a strain of Rinderpest virus in Turkey; upon his return, this strain proved inactive ( ...
... mumps and lymphocytic chorio-meningitis. Virus isolation had also been attempted. A salmonella reference service had been ...
"Lassa virus RefSeq Genome".. *^ Cornu, T. I.; De La Torre, J. C. (2001). "RING Finger Z Protein of Lymphocytic Choriomeningitis ... Dystroglycan as a Receptor for Lymphocytic Choriomeningitis Virus and Lassa Fever Virus". Science. 282 (5396): 2079-2081. doi: ... It shares this receptor with the prototypic Old World arenavirus lymphocytic choriomeningitis virus. Receptor recognition ... Lassa and lymphocytic choriomeningitis virus instead use an endocytotic pathway independent of clathrin, caveolin, dynamin and ...
... lymphocytic choriomeningitis virus), reovirus (Colorado tick virus), and henipaviruses.[10][11] The Powassan virus is a rare ... Causes of encephalitis include viruses such as herpes simplex virus and rabies as well as bacteria, fungi, or parasites.[1][2] ... Other causes of acute viral encephalitis are rabies virus, poliovirus, and measles virus.[9] ... Rozenberg, F; Deback C; Agut H (June 2011). "Herpes simplex encephalitis: from virus to therapy". Infectious Disorders Drug ...
... Virus from Pet Rodents. CDC, n.d. Web. 22 Sept. 2009. ---. Lymphocytic Choriomeningitis Virus ( ... "Lymphocytic Choriomeningitis Virus." Hotchin J, Weigand H (April 1961). "Studies of lymphocytic choriomeningitis in mice. I. ... "Lymphocytic choriomeningitis." Barton LL, Hyndman NJ (March 2000). "Lymphocytic choriomeningitis virus: reemerging central ... "Lymphocytic Choriomeningitis." CDC. "Lymphocytic Choriomeningitis Virus from Pet Rodents." Hotchin J (1977). "Experimental ...
Lymphocytic choriomeningitis. Lymphocytic choriomeningitis virus (LCMV). Malaria. Géineas Plasmodium. Marburg hemorrhagic fever ... Ionfhabhtú Human parainfluenza virus. Human parainfluenza viruses (HPIV). Hymenolepiasis. Hymenolepis nana and Hymenolepis ... Colorado tick fever virus (CTFV). Common cold (Acute viral rhinopharyngitis; Acute coryza). usually rhinoviruses and ... Dengue viruses (DEN-1, DEN-2, DEN-3 and DEN-4) - Flaviviruses. ... Hepatitis E Virus. Herpes simplex. Herpes simplex virus 1 and 2 ...
... includes Hepatitis D virus (not a true virus, but a subviral agent) Lassa virus (Arenaviridae) Lymphocytic choriomeningitis ... virus Extra small virus Goji berry chlorosis virus Harmonia axyridis virus 1 Hepelivirus Jingmen tick virus Le Blanc virus ... includes Yellow fever virus, West Nile virus, Hepatitis C virus, Dengue fever virus, Zika virus Family Fusariviridae Family ... includes Measles virus, Mumps virus, Nipah virus, Hendra virus, and NDV Family Pneumoviridae - includes RSV and Metapneumovirus ...
RNA virus. MeV Subacute sclerosing panencephalitis. LCV Lymphocytic choriomeningitis. Arbovirus encephalitis. Orthomyxoviridae ... DNA virus. HBV (B). RNA virus. CBV. HAV (A). HCV (C). HDV (D). HEV (E). HGV (G). ... The JC virus or John Cunningham virus is a type of human polyomavirus (formerly known as papovavirus). It was identified by ... DNA virus. HBV Hepatocellular carcinoma. HPV Cervical cancer. Anal cancer. Penile cancer. Vulvar cancer. Vaginal cancer. ...
Lymphocytic choriomeningitis Lymphocytic choriomeningitis virus rodents exposure to urine, feces, or saliva ... Eastern equine encephalitis virus, Venezuelan equine encephalitis virus, Western equine encephalitis virus horses, donkeys, ... Kyasanur Forest disease virus rodents, shrews, bats, monkeys tick bite La Crosse encephalitis La Crosse virus chipmunks, tree ... Barmah Forest virus kangaroos, wallabies, opossums mosquito bite Bird flu Influenza A virus subtype H5N1 wild birds, ...
A87.2) Lymphocytic choriomeningitis. *(A88.) Other viral infections of central nervous system, not elsewhere classified *(A88.1 ... B20-B24) Human immunodeficiency virus (HIV) disease[संपादित करें]. *(B20.) Human Immunodeficiency Virus (HIV) disease Resulting ... B24.) Unspecified Human Immunodeficiency Virus (HIV) Disease. (B25-B34) Other viral diseases[संपादित करें]. *(B25.) ... B21.) Human Immunodeficiency Virus (HIV) disease Resulting in malignant neoplasms *(B21.0) HIV disease resulting in Kaposi's ...
RNA virus. MeV Subacute sclerosing panencephalitis. LCV Lymphocytic choriomeningitis. Arbovirus encephalitis. Orthomyxoviridae ... DNA virus. HBV (B). RNA virus. CBV. HAV (A). HCV (C). HDV (D). HEV (E). HGV (G). ... DNA virus. HBV Hepatocellular carcinoma. HPV Cervical cancer. Anal cancer. Penile cancer. Vulvar cancer. Vaginal cancer. ... RNA virus. HCV Hepatocellular carcinoma. Splenic marginal zone lymphoma. HTLV-I Adult T-cell leukemia/lymphoma. ...
RNA virus. MeV Subacute sclerosing panencephalitis. LCV Lymphocytic choriomeningitis. Arbovirus encephalitis. Orthomyxoviridae ... DNA virus. HBV (B). RNA virus. CBV. HAV (A). HCV (C). HDV (D). HEV (E). HGV (G). ... The hepatitis C virus (HCV)[2] is a small (55-65 nm in size), enveloped, positive-sense single-stranded RNA virus of the family ... Hepatitis C virus is predominantly a blood-borne virus, with very low risk of sexual or vertical transmission.[47] Because of ...
Lymphocytic choriomeningitis(英语:Lymphocytic choriomeningitis). 虫媒病毒性脑炎. 正黏液病毒科 流行性腦炎. 狂犬病病毒 狂犬病. 钱迪普病毒(英语:Chandipura virus). 疱疹 ... CBV(英语:Coxsackie B virus). 甲型肝炎 (A). HCV(英语:Hepatitis C virus) (C). 丁型肝炎 (D). HEV(英语:Hepatitis E virus) (E). 庚型肝炎 (G). ... Yaba monkey tumor virus(英语:Yaba monkey tumor virus). *MCV(英语:Molluscum contagiosum virus) *Molluscum contagiosum(英语:Molluscum ... JC 病毒
RNA virus. MeV Subacute sclerosing panencephalitis. LCV Lymphocytic choriomeningitis. Arbovirus encephalitis. Orthomyxoviridae ... DNA virus. HBV (B). RNA virus. CBV. HAV (A). HCV (C). HDV (D). HEV (E). HGV (G). ... Further information: Influenza A virus subtype H7N9. Influenza A virus subtype H7N9 is a novel avian influenza virus first ... Until H5N1, all known avian influenza viruses had a Glu at position 627, while all human influenza viruses had a Lys.[15]. HA ...
RNA virus:MeV (Subacute sclerosing panencephalitis) · LCV(Lymphocytic choriomeningitis) · Arbovirus encephalitis · ... DNA virus: HBV (B). RNA virus: CBV · HAV (A) · HCV (C) · HDV (D) · HEV (E) · HGV (G) ... "Death toll from India's Nipah virus outbreak rises to 12 - Xinhua , English.news.cnDeath toll from India's Nipah virus outbreak ... DNA virus: HBV (Hepatocellular carcinoma) · HPV (Cervical cancer, Anal cancer) · Kaposi's sarcoma-associated herpesvirus ( ...
Lymphocytic choriomeningitis. *Solitary lymphatic nodule. History[edit]. Hippocrates, in the 5th century BC, was one of the ... It is associated with past infection with the Epstein-Barr Virus, and generally causes a painless "rubbery" lymphadenopathy. It ...
RNA virus. MeV Subacute sclerosing panencephalitis. LCV Lymphocytic choriomeningitis. Arbovirus encephalitis. Orthomyxoviridae ... DNA virus. HBV (B). RNA virus. CBV. HAV (A). HCV (C). HDV (D). HEV (E). HGV (G). ... Hepatitis B virus (HBV) is a member of the hepadnavirus family.[36] The virus particle (virion) consists of an outer lipid ... The virus gains entry into the cell by binding to NTCP [45] on the surface and being endocytosed. Because the virus multiplies ...
RNA virus. MeV Subacute sclerosing panencephalitis. LCV Lymphocytic choriomeningitis. Arbovirus encephalitis. Orthomyxoviridae ... DNA virus. HBV (B). RNA virus. CBV. HAV (A). HCV (C). HDV (D). HEV (E). HGV (G). ... Virus lifecycle[edit]. The lifecycle of hepatitis E virus is unknown; the capsid protein obtains viral entry by binding to a ... Recovery leads to virus clearance from the blood, while the virus may persist in stool for much longer. Recovery is also marked ...
RNA virus. MeV Subacute sclerosing panencephalitis. LCV Lymphocytic choriomeningitis. Arbovirus encephalitis. Orthomyxoviridae ... DNA virus. HBV (B). RNA virus. CBV. HAV (A). HCV (C). HDV (D). HEV (E). HGV (G). ... RNA viruses[edit]. Not all oncoviruses are DNA viruses. Some RNA viruses have also been associated such as the hepatitis C ... DNA viruses[edit]. *Human papilloma virus (HPV), a DNA virus, causes transformation in cells through interfering with tumor ...
"Timed appearance of lymphocytic choriomeningitis virus after gastric inoculation of mice". Archives of pathology & laboratory ... virus Ébola (EBOV, tamén chamado Zaire), virus Bundibugyo (BDBV), virus Sudán (SUDV), e virus Bosque Taï (TAFV, tamén chamado ... Inicialmente, creuse que o virus era o virus Marburg, pero despois foi identificado como un novo tipo de virus relacionado co ... Os primeiros ebolavirus que se descubriron foron o virus Ebola e o virus Sudán. Máis tarde apareceron os virus Reston, Taï ...
RNA virus. MeV Subacute sclerosing panencephalitis. LCV Lymphocytic choriomeningitis. Arbovirus encephalitis. Orthomyxoviridae ... DNA virus. HBV (B). RNA virus. CBV. HAV (A). HCV (C). HDV (D). HEV (E). HGV (G). ... DNA virus. HBV Hepatocellular carcinoma. HPV Cervical cancer. Anal cancer. Penile cancer. Vulvar cancer. Vaginal cancer. ... RNA virus. HCV Hepatocellular carcinoma. Splenic marginal zone lymphoma. HTLV-I Adult T-cell leukemia/lymphoma. ...
RNA virus. MeV Subacute sclerosing panencephalitis. LCV Lymphocytic choriomeningitis. Arbovirus encephalitis. Orthomyxoviridae ... DNA virus. HBV (B). RNA virus. CBV. HAV (A). HCV (C). HDV (D). HEV (E). HGV (G). ... Epstein-Barr virus snoRNA1 is a box CD-snoRNA generated by the virus during latency. V-snoRNA1 may act as a miRNA-like ... The Epstein-Barr virus was named after Michael Anthony Epstein and Yvonne Barr, who discovered the virus together with Bert ...
會引起腦膜炎的病毒包括腸病毒、單純皰疹II型病毒(I型病毒比較少見)、水痘帶狀皰狀病毒(導致水痘和帶狀皰疹的病毒)、
RNA virus: Subacute sclerosing panencephalitis - Lymphocytic choriomeningitis - Tick-borne meningoencephalitis teu katangén: ... DNA virus: HPV (Genital wart, Kanker sérviks). Virus RNA, retrovirus: HIV (AIDS, AIDS dementia complex) - Adult T-cell leukemia ... DNA virus, Herpesviridae: Herpes simplex - Chickenpox - Herpes zoster - KSHV DNA virus, liana: Poxviridae (Smallpox, Monkeypox ... RNA virus, IV: Acute viral nasopharyngitis - Severe acute respiratory syndrome RNA virus, V: Influenza/Avian influenza - Human ...
RNA virus. MeV Subacute sclerosing panencephalitis. LCV Lymphocytic choriomeningitis. Arbovirus encephalitis. Orthomyxoviridae ... DNA virus. HBV (B). RNA virus. CBV. HAV (A). HCV (C). HDV (D). HEV (E). HGV (G). ... Gastroenteritis viruses: A = rotavirus, B = adenovirus, C = norovirus and D = astrovirus. The virus particles are shown at the ... DNA virus. HBV Hepatocellular carcinoma. HPV Cervical cancer. Anal cancer. Penile cancer. Vulvar cancer. Vaginal cancer. ...
RNA virus:হাম (Subacute sclerosing panencephalitis) · LCV(Lymphocytic choriomeningitis) · Arbovirus encephalitis · ... DNA virus: HBV (হেপাটাইটিস বি). RNA virus: CBV · HAV (A) · HCV (C) · HDV (D) · HEV (E) · HGV (G) ... DNA virus: HBV (Hepatocellular carcinoma) · HPV (জরায়ুমুখের ক্যান্সার, Anal cancer) · Kaposi's sarcoma-associated herpesvirus ... RNA virus: HCV (Hepatocellular carcinoma) · HTLV-I (Adult T-cell leukemia/lymphoma) ...
RNA virus. MeV Subacute sclerosing panencephalitis. LCV Lymphocytic choriomeningitis. Arbovirus encephalitis. Orthomyxoviridae ... DNA virus. HBV (B). RNA virus. CBV. HAV (A). HCV (C). HDV (D). HEV (E). HGV (G). ... The mutation rate in the virus is relatively high even for an RNA virus with a synonymous substitution rate of 1.0 x 10−2 ... DNA virus. HBV Hepatocellular carcinoma. HPV Cervical cancer. Anal cancer. Penile cancer. Vulvar cancer. Vaginal cancer. ...
Lymphocytic choriomeningitis. Arbovirus encephalitis. ارتومیکسوویریده Encephalitis lethargica. RV هاری. Chandipura virus. ...
RNA virus. MeV Subacute sclerosing panencephalitis. LCV Lymphocytic choriomeningitis. Arbovirus encephalitis. Orthomyxoviridae ... DNA virus. HBV (B). RNA virus. CBV. HAV (A). HCV (C). HDV (D). HEV (E). HGV (G). ... The virus is subsequently absorbed into the bloodstream.[32] Known as viremia, the presence of a virus in the bloodstream ... DNA virus. HBV Hepatocellular carcinoma. HPV Cervical cancer. Anal cancer. Penile cancer. Vulvar cancer. Vaginal cancer. ...
Lymphocytic Choriomeningitis Virus (LCMV). Lymphocytic choriomeningitis (LCM) is a rare viral disease that can be transmitted ... Lymphocytic Choriomeningitis Virus Infection in Organ Transplant Recipients - Massachusetts, Rhode Island, 2005. Morbidity and ... Monkeypox Virus *not currently in the United States. Monkeypox is a rare viral disease that usually found in central and ... Although the virus is not currently reported in the United States, people can become infected if they are bitten or come in ...
... is a single-stranded RNA virus of the family Arenaviridae. Other members of Arenaviridae include Lassa, Argentine hemorrhagic ... fever, Bolivian hemorrhagic fever, and Venezuelan hemorrhagic fever viruses. ... encoded search term (Pediatric Lymphocytic Choriomeningitis Virus) and Pediatric Lymphocytic Choriomeningitis Virus What to ... Lymphocytic choriomeningitis virus (LCMV) is a single-stranded RNA virus of the family Arenaviridae. Other members of ...
... is a single-stranded RNA virus that belongs to the family Arenaviridae (so named because of its appearance on electron ... microscopy, which resembles grains of sand). Other members of this family include Lassa virus and the Tacaribe group. ... encoded search term (Lymphocytic Choriomeningitis Virus (LCMV) Infection) and Lymphocytic Choriomeningitis Virus (LCMV) ... Lymphocytic Choriomeningitis Virus (LCMV) Infection. Updated: Sep 11, 2017 * Author: Philip J McDonald, MD; Chief Editor: Burke ...
Lymphocytic Choriomeningitis Virus Transmitted Through Solid Organ Transplantation --- Massachusetts, 2008. Lymphocytic ... Transmission of lymphocytic choriomeningitis virus by organ transplantation. N Engl J Med 2006;354:2235--49. ... hepatitis B and C viruses (HBV and HCV), human T-lymphotropic virus, and syphilis. In addition, HIV, HBV, and HCV nucleic acid ... choriomeningitis virus (LCMV) is a rodent-borne arenavirus found worldwide. House mice (Mus musculus) are the natural reservoir ...
Lymphocytic Choriomeningitis Virus: Reemerging Central Nervous System Pathogen Message Subject (Your Name) has sent you a ... Lymphocytic Choriomeningitis Virus: Reemerging Central Nervous System Pathogen. Leslie L. Barton, N. Joanne Hyndman ...
... the RNA virus that causes lymphocytic choriomeningitis; infects mice and monkeys and dogs and guinea pigs and human beings ... n. the RNA virus that causes lymphocytic choriomeningitis; infects mice and monkeys and dogs and guinea pigs and human beings ...
The pathology of the disease10 supports the current view that an immune reaction by the host against the virus or a virus ... is what the consequences might be for virus infected cells in the event of an immune response against the virus or a virus ... A similar mechanism may be important in the chronic autoimmune-like disease in virus carrier mice2,5. Further elucidation of ... Here we report preliminary experiments involving transplantation of cells from virus carrier mice to syngeneic non-infected ...
2005) Dual role of the lymphocytic choriomeningitis virus intergenic region in transcription termination and virus propagation ... 1936) An epidemic in a mouse colony due to the virus of acute lymphocytic choriomeningitis. J Exp Med 63:533-546. ... 1996) Lymphocytic choriomeningitis virus: A neglected central nervous system pathogen. Clin Infect Dis 22:197. ... 1993) Quantification of lymphocytic choriomeningitis virus with an immunological focus assay in 24-well plates. ALTEX 10:6-14. ...
Lymphocytic choriomeningitis virus in man. Serologic evidence of association with pet hamsters;. Download Prime PubMed App to ... Lymphocytic-choriomeningitis-virus infection traced to a pet hamster.. *Lymphocytic choriomeningitis in university hospital ... TestsCricetinaeFemaleFluorescent Antibody TechniqueHumansLymphocytic ChoriomeningitisLymphocytic choriomeningitis virusMale ... Some observations on hamster-derived human infection with lymphocytic choriomeningitis virus.. *Radioimmunoassay for LCM virus ...
Replication-defective lymphocytic choriomeningitis virus vectors expressing guinea pig cytomegalovirus gB and pp65 homologs are ... To determine the efficacy of replication-defective lymphocytic choriomeningitis virus (rLCMV) vectors expressing the guinea pig ...
... and negative results for other agents and confirmed by a positive PCR result for lymphocytic choriomeningitis virus in an ... We report congenital microencephaly caused by infection with lymphocytic choriomeningitis virus in the fetus of a 29-year-old ... Wright R, Johnson D, Neumann M, Ksiazek TG, Rollin P, Keech RV, et al. Congenital lymphocytic choriomeningitis virus syndrome: ... Congenital lymphocytic choriomeningitis virus: when to consider the diagnosis. J Child Neurol. 2014;29:837-42. DOIPubMed ...
Congenital lymphocytic choriomeningitis virus: an unrecognized teratogenic pathogen. Emerg Infect Dis. 1995;1:152-3. DOIPubMed ... Lymphocytic choriomeningitis virus: reemerging central nervous system pathogen. Pediatrics. 2000;105:e35-6. DOIPubMed ... Lymphocytic choriomeningitis virus meningitis, New York, NY, USA, 2009. Emerg Infect Dis. 2010;16:328-30.PubMed ... Human Infection with Lymphocytic Choriomeningitis Virus. Emerging Infectious Diseases. 2010;16(6):1046-1047. doi:10.3201/ ...
... we focused on two RNA viruses: lymphocytic choriomeningitis (LCMV) and influenza (Flu). Many of the currently established ... virus-induced immunosuppression, virus-induced autoimmunity, immune complex disease, and virus-lymphocyte and virus-dendritic ... virus-induced immunosuppression, virus-induced autoimmunity, immune complex disease, and virus-lymphocyte and virus-dendritic ... we focused on two RNA viruses: lymphocytic choriomeningitis (LCMV) and influenza (Flu). Many of the currently established ...
adenoviral vector expressing lymphocytic choriomeningitis virus glycoprotein. Ad-IiGP. adenoviral vector expressing lymphocytic ... lymphocytic choriomeningitis virus. pfp. perforin deficient. pfp/ifn. perforin/IFN-γ double deficient. p.i.. postinfection. WT ... Vaccination against Lymphocytic Choriomeningitis Virus Infection in MHC Class II-Deficient Mice. Peter Johannes Holst, Jan ... Quantification of lymphocytic choriomeningitis virus with an immunological focus assay in 24- or 96-well plates. J. Virol. ...
... we reported that Sp-MØ down-regulated their ability to cross-present the cell-associated lymphocytic choriomeningitis virus ... We also addressed the capacity of diverse LCMV antigens, generated during virus infection, to induce LCMV-specific CTL ... responses via cross-presentation by employing antigen donor cells (ADCs) that provide sufficient LCMV antigens after virus ...
Publications about Experts and Doctors on lymphocytic choriomeningitis virus in Atlanta, Georgia, United States ... lymphocytic choriomeningitis virus*lymphocytic choriomeningitis*arenaviridae infections*radiation chimera*interleukin 7 ... Qualitatively different memory CD8+ T cells are generated after lymphocytic choriomeningitis virus and influenza virus ... Experts and Doctors on lymphocytic choriomeningitis virus in Atlanta, Georgia, United States. Summary. Locale: Atlanta, Georgia ...
... virus infection. Download Prime PubMed App to iPhone, iPad, or Android ... Ultrastructural pathology of glomerular lesions in gnotobiotic mice with congenital lymphocytic choriomeningitis (LCM) ... ViralKidney DiseasesKidney GlomerulusLymphocytic ChoriomeningitisLymphocytic choriomeningitis virusMiceMicroscopy, Electron ... Congenital lymphocytic choriomeningitis virus infection in gnotobiotic mice.. *Pathogenesis of lesions in lymphoid tissue of ...
Hepatitis C und HIV stellen wegen des Trägertums durch Tiere oder Menschen ein Virus-Reservoir dar, welches eine zunehmende ... Role of the virus nucleoprotein in the regulation of lymphocytic choriomeningitis virus transcription and RNA replication. J. ... Isolation of a recombinant lymphocytic choriomeningitis virus expressing the glycoprotein of vesicular stomatitis virus. ... Generation of recombinant lymphocytic choriomeningitis virus from plasmids GRS-060/00 CHF 39000 Daniel Pinschewer 01.2001 - ...
... the potential role of ADAR1-L in the innate immune response to lymphocytic choriomeningitis virus (LCMV), a widely used virus ... A-to-G Hypermutation in the Genome of Lymphocytic Choriomeningitis Virus. Roland C. Zahn, Ina Schelp, Olaf Utermöhlen, Dorothee ... A-to-G Hypermutation in the Genome of Lymphocytic Choriomeningitis Virus. Roland C. Zahn, Ina Schelp, Olaf Utermöhlen, Dorothee ... A-to-G Hypermutation in the Genome of Lymphocytic Choriomeningitis Virus. Roland C. Zahn, Ina Schelp, Olaf Utermöhlen, Dorothee ...
The cellular receptor for lymphocytic choriomeningitis virus (LCMV), Lassa fever virus (LFV), and several other arenaviruses is ... Characterization of lymphocytic choriomeningitis virus-binding protein(s): a candidate cellular receptor for the virus. J Virol ... Identification of alpha-dystroglycan as a receptor for lymphocytic choriomeningitis virus and Lassa fever virus. Science 282 ... Infection of Dendritic Cells by Lymphocytic Choriomeningitis Virus. In: Steinkasserer A. (eds) Dendritic Cells and Virus ...
... and tumor cell rejection in mice persistently infected with lymphocytic choriomeningitis virus.. J F Bukowski, C A Biron and R ... and resistance to tumor implants were studied in mice persistently infected with lymphocytic choriomeningitis virus (LCMV). NK ... and tumor cell rejection in mice persistently infected with lymphocytic choriomeningitis virus. ... and tumor cell rejection in mice persistently infected with lymphocytic choriomeningitis virus. ...
... ... It has been previously reported that Lymphocytic Choriomeningitis nucleoprotein expressed in HEK cells (HEK-NP) could be cross ...
... lymphocytic choriomeningitis virus; rhsp 70, recombinant human hsp70; VSV, vesicular stomatitis virus. ... 1976) Antibody-complement interactions with purified lymphocytic choriomeningitis virus. Virology 73:59-71, pmid:986722.. ... Immunization with a Lymphocytic Choriomeningitis Virus Peptide Mixed with Heat Shock Protein 70 Results in Protective Antiviral ... Immunization with a Lymphocytic Choriomeningitis Virus Peptide Mixed with Heat Shock Protein 70 Results in Protective Antiviral ...
Induction and Exhaustion of Lymphocytic Choriomeningitis Virus-specific Cytotoxic T Lymphocytes Visualized Using Soluble ... Induction and Exhaustion of Lymphocytic Choriomeningitis Virus-specific Cytotoxic T Lymphocytes Visualized Using Soluble ... Cytotoxic activity and virus titers in mice infected intravenously with LCMV-DOCILE. LCMV-specific CTL activity was measured ... Cytotoxic activity and virus titers in mice infected intracranially with LCMV-DOCILE. LCMV-specific CTL activity was measured ...
... we engineered a panel of chimeric Lassa/lymphocytic choriomeningitis viruses. Mutants carrying a viral envelope with the ... Starting from an emerging virus in a wild-type mouse, ... of recombinant lymphocytic choriomeningitis virus/Lassa virus ... Here, we describe gain-of-function mutations of lymphocytic choriomeningitis virus (LCMV) expressing Lassa virus (LASV) GP, ... we engineered a panel of chimeric Lassa/lymphocytic choriomeningitis viruses. Mutants carrying a viral envelope with the ...
Selection of genetic variants of lymphocytic choriomeningitis virus in spleens of persistently infected mice. Role in ... EM011-treated mice mount normal T-cell responses to an acute lymphocytic choriomeningitis virus infection. Ritu Aneja, Vandana ... importance of single amino acid changes in the polymerase and glycoprotein of lymphocytic choriomeningitis virus. J Virol 1993; ... we chose to use the well-established model of murine infection with lymphocytic choriomeningitis virus (LCMV). This model ...
Lymphocytic Choriomeningitis Lymphocytic Choriomeningitis Virus Mice Microcephaly Pregnancy Pregnancy Complications, Infectious ... A case of lymphocytic choriomeningitis virus (LCMV) infection led to investigation of the reservoir. LCMV was detected in mice ... Lymphocytic choriomeningitis virus (LCMV) was detected in 2 patients with acute meningitis in southern Spain within a 3-year ... This report describes several events in which virus isolation and identification by electron microscopy were critical to i... ...
Antivirally protective cytotoxic T cell memory to lymphocytic choriomeningitis virus is governed by persisting antigen. - S ... was investigated in vivo and in vitro using lymphocytic choriomeningitis virus (LCMV) and recombinant vaccinia viruses ... Antivirally protective cytotoxic T cell memory to lymphocytic choriomeningitis virus is governed by persisting antigen.. ... Protection against lethal choriomeningitis is less sensitive to assess memory because it depends upon high levels of CTL ...
T-cell-mediated immunity to lymphocytic choriomeningitis virus in beta2-integrin (CD18)- and ICAM-1 (CD54)-deficient mice.. J P ... The T-cell response to lymphocytic choriomeningitis virus was studied in mice with deficient expression of beta2-integrins or ... T-cell-mediated immunity to lymphocytic choriomeningitis virus in beta2-integrin (CD18)- and ICAM-1 (CD54)-deficient mice. ... T-cell-mediated immunity to lymphocytic choriomeningitis virus in beta2-integrin (CD18)- and ICAM-1 (CD54)-deficient mice. ...
We undertook evaluation of a novel bivalent vaccine based on nonreplicating lymphocytic choriomeningitis virus (rLCMV) vectors ... Congenital Cytomegalovirus Conferred by Combined Glycoprotein B/pp65 Vaccination Using a Lymphocytic Choriomeningitis Virus ... Keywords: congenital cytomegalovirus; cytomegalovirus; fetal infection; live vector vaccines; lymphocytic choriomeningitis ...
  • Lymphocytic choriomeningitis virus (LCMV) is a single-stranded RNA virus of the family Arenaviridae. (medscape.com)
  • Lymphocytic choriomeningitis virus (LCMV) was first isolated in 1933 from a patient suspected to have St. Louis Encephalitis . (medscape.com)
  • Lymphocytic choriomeningitis virus (LCMV) is a rodent-borne arenavirus found worldwide. (cdc.gov)
  • On April 22, archived whole blood collected on the day of death had evidence of acute LCMV infection by PCR and virus isolation. (cdc.gov)
  • However, the prototypic arenavirus lymphocytic choriomeningitis virus (LCMV) has proven to be a Rosetta stone for the investigation of virus-host interactions. (pnas.org)
  • The ability to generate recombinant LCMV (rLCMV) expressing additional foreign genes of interest would open novel avenues for the study of virus-host interactions and the development of novel vaccine strategies and high-throughput screens to identify antiarenaviral molecules. (pnas.org)
  • Thus, the prototypic arenavirus lymphocytic choriomeningitis virus (LCMV) has proven to be a superb workhorse in the field of virology and immunology ( 1 ). (pnas.org)
  • Reverse genetics systems have been now developed for LCMV ( 11 ), LASV ( 12 ), and Tacaribe virus ( 13 ), which are allowing investigators to examine the cis -acting sequences and trans -acting factors that control arenavirus replication and gene expression, as well as assembly and budding ( 14 ). (pnas.org)
  • Lymphocytic choriomeningitis virus (LCMV) is an arenavirus, discovered by Armstrong and Lillie in 1933 ( 1 ) that chronically infects small rodents. (cdc.gov)
  • Because of these negative results for virus infections, an ascitic fluid sample was tested by PCR for LCMV. (cdc.gov)
  • I read with great interest the article regarding lymphocytic choriomeningitis virus (LCMV) meningitis in a New York City resident ( 1 ). (cdc.gov)
  • To describe the pathogenesis and significance of viral infection in terms of host responses, resultant injury, and therapy, we focused on two RNA viruses: lymphocytic choriomeningitis (LCMV) and influenza (Flu). (pnas.org)
  • Many of the currently established concepts and consequences about viruses and immunologic tolerance, virus-induced immunosuppression, virus-induced autoimmunity, immune complex disease, and virus-lymphocyte and virus-dendritic cell interactions evolved through studies of LCMV in its natural murine host. (pnas.org)
  • In this study, we demonstrated that a broad CD8 + T cell immune response could be elicited in MHC class II-deficient mice by vaccination with adenovirus encoding lymphocytic choriomeningitis virus (LCMV) glycoprotein tethered to MHC class II-associated invariant chain. (jimmunol.org)
  • In contrast, vaccination with adenovirus encoding unlinked LCMV glycoprotein induced weak virus control in the absence of CD4 + T cells, and mice may die of increased immunopathology associated with incomplete protection. (jimmunol.org)
  • The extent to which virus or vaccine induced CD8 + T cells can contribute to the control of chronic virus infection in the absence or presence of CD4 + T cell help has been addressed by transfer experiments of sorted cell populations into carriers of different strains of lymphocytic choriomeningitis virus (LCMV) ( 12 , 13 ). (jimmunol.org)
  • As a result of examining the antigen presentation of Sp-MØ during differentiation, we reported that Sp-MØ down-regulated their ability to cross-present the cell-associated lymphocytic choriomeningitis virus nucleoprotein (LCMV-NP) but not the soluble OVA proteins without altering their capacity to directly present LCMV antigens after infection. (queensu.ca)
  • We also addressed the capacity of diverse LCMV antigens, generated during virus infection, to induce LCMV-specific CTL responses via cross-presentation by employing antigen donor cells (ADCs) that provide sufficient LCMV antigens after virus inactivation with no possible direct antigen presentation. (queensu.ca)
  • Ziel dieses Projektes ist es, basierend auf molekularvirologischen Methoden, die Technologie zu erarbeiten, um LCMV gänzlich aus Plasmid-cDNA und somit nach exaktem, willentlich festgelegtem „Bauplan" herzustellen, um Viren zu erhalten, welche sich zum Beispiel nur in einer einzigen Determinante vom „normalen" Virus unterscheiden oder Determinanten fremder Viren anstelle der eigenen aufweisen. (grstiftung.ch)
  • Mit diesen rekombinanten Viren soll in einem nächsten Schritt der Infektionsverlauf und die Immunantwort in der Maus studiert werden, um insbesondere die Frage zu erläutern, warum LCMV die Fähigkeit hat, sich einer effektiven Abwehr durch Antikörper zu entziehen, welche ein Persistieren vergleichbarer Viren wie dem Vesikulären Stomatitis Virus (VSV) verhindert. (grstiftung.ch)
  • Entsprechende Techniken wurden zwar für verschiedene Virusfamilien bereits erfolgreich erarbeitet und angewendet, doch ist es bislang noch keiner Forschergruppe geglückt, in ähnlicher Weise ein Virus aus der Familie der Arenaviren, wozu auch LCMV gehört, genetisch zu verändern. (grstiftung.ch)
  • Here, the potential role of ADAR1-L in the innate immune response to lymphocytic choriomeningitis virus (LCMV), a widely used virus model, was studied. (asm.org)
  • In this study, we analyzed the genome of the lymphocytic choriomeningitis virus (LCMV), an arenavirus, for mutations indicative of the antiviral activity of ADAR1-L in vivo. (asm.org)
  • The cellular receptor for lymphocytic choriomeningitis virus (LCMV), Lassa fever virus (LFV), and several other arenaviruses is α-dystroglycan (α-DG). (springer.com)
  • 10%), rarely replicate in the white pulp, and generate a robust anti-LCMV CTL response that clears the virus infection. (springer.com)
  • To assess the effects of chronic virus infection on NK cells, the related phenomena of interferon (IFN) production, NK cell activation, and resistance to tumor implants were studied in mice persistently infected with lymphocytic choriomeningitis virus (LCMV). (jimmunol.org)
  • The plasma from LCMV-carrier mice contained an antiviral substance identified as IFN type I, based on species specificity, virus nonspecificity, resistance to pH 2, and sensitivity to antibody to type I IFN. (jimmunol.org)
  • A CTL epitope from the well-defined lymphocytic choriomeningitis virus (LCMV) system was mixed with recombinant hsp70 in vitro under conditions that optimize peptide binding to hsp70. (rupress.org)
  • Cytotoxic activity and virus titers in mice infected intravenously with LCMV-DOCILE. (rupress.org)
  • Here, we describe gain-of-function mutations of lymphocytic choriomeningitis virus (LCMV) expressing Lassa virus (LASV) GP, which can prolong viremia in mice depending on the sequences in the GP-2 cytoplasmic tail. (nih.gov)
  • Further replacement of the entire sequence of the cytosolic tail from LASV to LCMV GP resulted in increased viral titers and delayed clearance of the viruses. (nih.gov)
  • The basis of antiviral protection by memory cytotoxic T lymphocytes (CTL) was investigated in vivo and in vitro using lymphocytic choriomeningitis virus (LCMV) and recombinant vaccinia viruses expressing the LCMV- glycoprotein (vacc-GP) or - nucleoprotein (vacc-NP). (curehunter.com)
  • In contrast, antiviral protection measured as the capacity of the primed host to reduce virus titers after challenge infection correlated with elevated CTLp frequencies after immunization with live LCMV or recombinant vaccinia virus-expressing the major LCMV epitope. (curehunter.com)
  • CTLp frequencies were constantly increased up to 70 d for LCMV immune mice, but rapidly decreased a few weeks after immunization with low dose vaccinia recombinant virus. (curehunter.com)
  • Hickerson, Brady T;Westover, Jonna B;Jung, Kie-Hoon;Komeno, Takashi;Furuta, Yousuke;Gowen, Brian B 2018-05-14 00:00:00 Abstract Lymphocytic choriomeningitis virus (LCMV) poses a substantial risk to immunocompromised individuals. (deepdyve.com)
  • Abstract Lymphocytic choriomeningitis virus (LCMV) poses a substantial risk to immunocompromised individuals. (deepdyve.com)
  • Lymphocytic choriomeningitis virus (LCMV) is carried by wild mice. (cdc.gov)
  • The resulting antisera cross-reacted completely with antisera against lymphocytic choriomeningitis virus (LCMV) in an immunoradiometric assay employing BHK-21F antigen. (uu.nl)
  • Persistent infections by LCMV can be maintained without expression of extracellular virus particles and without appearance of large amounts of viral antigens on the cell surface. (uu.nl)
  • In laboratories working with the lymphocytic choriomeningitis virus (LCMV), the virus can be transmitted to humans through needlestick injury and lead to serious acute illness up to meningitis. (biomedcentral.com)
  • It highlights the importance of infection prevention practices that comprise particularly well established safety precaution protocols in research laboratories handling this pathogenic virus, because exposure to even a small amount of LCMV can lead to a severe, life-threatening infection. (biomedcentral.com)
  • This highlights the importance of infect prevention strategies that comprise particularly well established safety precaution protocols in research laboratories handling this pathogenic virus, because exposure to LCMV can lead to a life-threatening infection. (biomedcentral.com)
  • The needle stuck on a syringe that was used to infect mice with Lymphocytic choriomeningitis virus (LCMV) variant clone-13. (biomedcentral.com)
  • Lymphocytic choriomeningitis virus (LCMV) produces a persistent infection of the nervous system in susceptible mice. (elsevier.com)
  • Lymphocytic choriomeningitis virus (LCMV), strain WE, is a non-cytopathic RNA virus that is highly adapted to its natural host, the mouse. (ox.ac.uk)
  • Here we demonstrate LCMV-WE persistence at low levels after infection with 10(2) or 10(6) plaque-forming units, shown as viral genome, viral antigen, and replicative virus using sensitive in vitro and in vivo assays. (ox.ac.uk)
  • The path that the arenavirus lymphocytic choriomeningitis virus (LCMV) uses to enter rodent fibroblastic cell lines was dissected by infectivity and inhibition studies and immunoelectron microscopy. (ox.ac.uk)
  • We studied the mechanism of lymphocytic choriomeningitis virus (LCMV) persistence and the suppression of cytotoxic T lymphocyte (CTL) responses in BALB/c WEHI mice infected at birth with LCMV Armstrong strain. (semanticscholar.org)
  • Lymphocytic choriomeningitis virus (LCMV) is a ubiq- from 665 specimens. (cdc.gov)
  • To explore this concept, we have developed a recombinant lymphocytic choriomeningitis virus (LCMV) (rLCMVΔGP/GFP) where we replaced the viral glycoprotein (GP) with the green fluorescent protein (GFP). (scripps.edu)
  • PCR (RT-PCR) for LCMV was negative, indicating that specimens were inoculated onto cell culture to isolate virus, viremia was no longer present. (cdc.gov)
  • We used the infection of mice with lymphocytic choriomeningitis virus (LCMV) as a model to study how pathogens induce weight loss. (ulster.ac.uk)
  • If LCMV is introduced into the CNS of CTL-deficient mice, the immune response against the virus leads to a severe weight loss called wasting disease. (ulster.ac.uk)
  • Overall, this work defines the critical role of virus-primed CD4 T cells and of proinflammatory cytokines in the pathogenesis of wasting disease induced by LCMV infection. (ulster.ac.uk)
  • In addition, A549 cells infected with rLCMV/NP*(D382A), but not with WT LCMV, produced IFN and failed to rescue replication of the IFN-sensitive Newcastle disease virus. (pubmedcentralcanada.ca)
  • Moreover, compelling evidence indicates that the worldwide-distributed prototypic arenavirus lymphocytic choriomeningitis virus (LCMV) is a neglected human pathogen of clinical significance ( 23 , 32 ) and poses a special threat to immunocompromised individuals, as illustrated by cases of transplant-associated infections by LCMV with fatal outcomes ( 10 , 36 , 40 ). (pubmedcentralcanada.ca)
  • Mice persistently infected with LCMV exhibit relative high levels of virus replication in many tissues, but only very modest levels of type I interferon (IFN-I) are detected in sera from these mice ( 4 ). (pubmedcentralcanada.ca)
  • These findings would suggest that LCMV is able to modulate the innate defense responses of its natural host, the mouse, to favor virus long-term persistence. (pubmedcentralcanada.ca)
  • The attachment of lymphocytic choriomeningitis virus (LCMV) to murine and primate cell lines was quantitated by a fluorescence-activated cell sorter assay in which binding of biotinylated virus was detected with streptavidin-fluorescein isothiocyanate. (ox.ac.uk)
  • It was also blocked by LCMV-specific antiserum and a neutralizing monoclonal antibody to the virus glycoprotein GP-1 but not by antibodies specific for GP-2, indicating that attachment was likely mediated by GP-1. (ox.ac.uk)
  • Treatment of cells with any of several proteases abolished LCMV binding, whereas phospholipases including phosphatidylinositol-specific phospholipase C had no effect, indicating that one or more membrane proteins were involved in virus attachment. (ox.ac.uk)
  • Virus bound to protein(s) with a molecular mass of 120 to 140 kDa in membranes from cell lines permissive for LCMV but not from nonpermissive cell lines. (ox.ac.uk)
  • Binding was specific, since unlabeled LCMV, but not the unrelated enveloped virus herpes simplex virus type 1, competed with 125I-labeled LCMV for binding to the 120- to 140-kDa band. (ox.ac.uk)
  • The proteinaceous nature of the LCMV-binding substance was confirmed by the lack of virus binding to proteinase K-treated membrane components. (ox.ac.uk)
  • Hence, LCMV attachment to rodent fibroblastic cell lines is mediated by a glycoprotein(s) with a molecular mass of 120 to 140 kDa, with complex N-linked sugars that are not involved in virus binding. (ox.ac.uk)
  • Conversation This study in the LCMV model demonstrates enhanced intracellular replication-a house lent from the L1079 mutation in the Cl13 polymerase-is a primary determinant of viral chronicity and exhaustion of the virus-specific T-cell response as well as generalized immunosuppression. (lecollege.org)
  • We acknowledge that DCs not merely are initial goals of LCMV an infection (38) dispersing chlamydia to various other cell types through the entire body (42) but also signify the primary cell type priming LCMV-specific CTLs in vivo (43) presumably after virus-induced phenotypic transformation of pDCs into Compact disc11c high-expressing traditional DCs (44). (lecollege.org)
  • Analyses with segmental reassortants of lymphocytic choriomeningitis virus (LCMV) RNA have shown that cytotoxic T lymphocytes (CTL) are induced by and recognize proteins encoded by the viral short segment, which specifies two virus structural proteins, glycoprotein (GP) and nucleoprotein (NP). (scripps.edu)
  • We have used LCMV-specific H2bb-restricted CTL clones and a family of serial C-terminal truncations of the LCMV GP expressed in vaccinia virus to map the precise specificities of the anti-GP clones. (scripps.edu)
  • Its causative agent is the lymphocytic choriomeningitis virus (LCMV), a member of the family Arenaviridae that was initially isolated in 1933. (news-medical.net)
  • The article highlights a case of a congenital lymphocytic choriomeningitis virus (LCMV) in an infant in upstate New York. (ebscohost.com)
  • LCMV is an arena-virus carried by domestic house mice as well as laboratory and pet rodents. (ebscohost.com)
  • The article discusses research published in a 2014 issue of the journal "Emerging Infectious Diseases" which found that infections are limited in an outbreak of lymphocytic choriomeningitis virus (LCMV) at three U.S. rodent-breeding facilities in 2012. (ebscohost.com)
  • Role of Lymphocytic Choriomeningitis Virus (LCMV) in Understanding Viral Immunology: Past, Present and Future. (ebscohost.com)
  • Lymphocytic choriomeningitis virus (LCMV) is a common infection of rodents first identified over eighty years ago in St. Louis, MO, U.S.A. It is best known for its application in immunological studies. (ebscohost.com)
  • Shows that the suppression of neutralizing-antibody responses in lymphocytic choriomeningitis virus (LCMV) infections in mice is due to selective infection of neutralizing-antibody-producing B cells by this non-cytopathic virus, and their subsequent destruction by virus-specific cytotoxic T. (ebscohost.com)
  • To clarify the presence of lymphocytic choriomeningitis virus (LCMV) in Spain, we examined blood and tissue specimens from 866 small mammals. (ebscohost.com)
  • A letter to the editor is presented in response to an article on lymphocytic choriomeningitis virus (LCMV) meningitis. (ebscohost.com)
  • Presents a study which determined the prevalence of lymphocytic choriomeningitis virus (LCMV) in human and rodent populations and to delineate the clinical spectrum of congenital infection. (ebscohost.com)
  • A peripheral membrane protein that is interactive with lymphocytic choriomeningitis virus (LCMV) was purified from cells permissive to infection. (ox.ac.uk)
  • Several strains of LCMV and other arenaviruses, including Lassa fever virus (LFV), Oliveros, and Mobala, bound to purified alpha-DG protein. (ox.ac.uk)
  • Wild-caught animals may act as carriers for such viruses as hantavirus and lymphocytic choriomeningitis (LCMV) depending on where they were captured. (csbsju.edu)
  • Lymphocytic choriomeningitis virus (LCMV), the prototype arenavirus, and Lassa virus (LASV), the causative agent of Lassa fever (LF), have considerable strain diversity and significant variations in pathogenicity for human beings and experimental pets. (saracatinib.info)
  • Intro The Old Globe (OW) band of the contains the prototypic lymphocytic choriomeningitis pathogen (LCMV) and Lassa pathogen (LASV), the causative agent of Lassa fever (LF), which may be the most widespread viral hemorrhagic fever (VHF) in Western world Africa (20). (saracatinib.info)
  • To evaluate the impact of immunodominance on CD8 T-cell properties, we compared the functional properties of dominant and subdominant populations in the response to lymphocytic choriomeningitis virus (LCMV). (ox.ac.uk)
  • Persistent infection of C3H/St mice with certain strains of lymphocytic choriomeningitis virus (LCMV) causes a growth hormone (GH) deficiency syndrome (GHDS) manifested as growth retardation and hypoglycemia. (jenner.ac.uk)
  • Virus clones isolated from the GHDS-negative LCMV WE strain can cause the disease, while others cannot. (jenner.ac.uk)
  • The purpose of the present study was to further elucidate the anti-viral spectrum of PAP by examining its effects on the survival of mice challenged with lymphocytic choriomeningitis virus (LCMV). (springer.com)
  • Lymphocytic choriomeningitis virus (LCMV), which is one of several arenaviruses that are pathogenic for humans, causes encephalitis and meningitis in man. (uab.edu)
  • In addition to these hemor- rhagic fever viruses, the arenavirus lymphocytic choriomeningitis virus (LCMV) can infect humans worldwide, although the illness is most often less disabling and severe than those elicited by the other arenaviruses. (springer.com)
  • Cases have been caused by emerging pathogens, including West Nile virus (WNV) (7, 8), rabies virus (9), lymphocytic choriomeningitis virus (LCMV) (10), and Balamuthia mandrillaris amebae (11). (thefreelibrary.com)
  • To address this issue, we reduced the precursor frequency of autoreactive T-cells in NOD mice through allelic exclusion induced by transgenic expression of an H2-D b class I−restricted T-cell receptor (TCR) specific for a pathologically irrelevant lymphocytic choriomeningitis virus (LCMV) peptide. (diabetesjournals.org)
  • Hamsters - Hamsters and other "pocket pets" can carry lymphocytic choriomeningitis virus (LCMV). (cnn.com)
  • This is a view of a modified lymphocytic choriomeningitis virus (LCMV). (eurekalert.org)
  • The researchers built artificial viruses based on lymphocytic choriomeningitis virus (LCMV), which can infect both rodents and humans. (eurekalert.org)
  • We report congenital microencephaly caused by infection with lymphocytic choriomeningitis virus in the fetus of a 29-year-old pregnant women at 23 weeks' gestation. (cdc.gov)
  • Other members of Arenaviridae include Lassa, Argentine hemorrhagic fever, Bolivian hemorrhagic fever, and Venezuelan hemorrhagic fever viruses. (medscape.com)
  • Other members of the Arenaviridae family include the Lassa virus (LASV) and the New World complex viruses (Junin, Machupo, Guanarito, Sabia). (medscape.com)
  • The Old World arenavirus Lassa virus (LASV) poses the highest public health concern among HF arenaviruses. (pnas.org)
  • Starting from an emerging virus in a wild-type mouse, we engineered a panel of chimeric Lassa/lymphocytic choriomeningitis viruses. (nih.gov)
  • Several arenaviruses, chiefly Lassa virus (LASV) and Junin virus in West Africa and Argentina, respectively, cause hemorrhagic fever (HF) disease in humans that is associated with high morbidity and significant mortality. (scripps.edu)
  • Several arenaviruses, chiefly Lassa virus (LASV), cause hemorrhagic fever disease in humans and represent a serious public health problem in their areas of endemicity ( 3 , 31 , 39 ). (pubmedcentralcanada.ca)
  • Identification of alpha-dystroglycan as a receptor for lymphocytic choriomeningitis virus and Lassa fever virus. (ox.ac.uk)
  • Arenaviruses cause several human diseases known primarily as the hemorrhagic fevers occurring in South and Latin America (Bolivia: Machupo, Argentine, Junin virus, and Brazil: Sabia virus) and in Africa (Lassa fever virus). (springer.com)
  • The S segment encodes for the virus nucleoprotein and glycoprotein, whereas the L segment encodes for the virus polymerase (L) and Z protein. (pnas.org)
  • VSV pseudotyped with the glycoprotein GP of the lymphocytic choriomeningitis virus (VSV-GP) has completely lost neurotropism and is perfectly safe in mouse models, while maintaining replication fitness.Here, the potential of VSV-GP as a vaccine vector was analyzed with ovalbumin (OVA) as a model antigen. (uibk.ac.at)
  • A single amino acid change in the glycoprotein of lymphocytic choriomeningitis virus is associated with the ability to cause growth hormone deficiency syndrome. (jenner.ac.uk)
  • Role of the stable signal peptide and cytoplasmic domain of G2 in regulating intracellular transport of the Junin virus envelope glycoprotein complex. (springer.com)
  • Bitopic membrane topology of the stable signal peptide in the tripartite Junin virus Gp-C envelope glycoprotein complex. (springer.com)
  • Beyer WR, Popplau D, Garten W, Von Laer D, Lenz O. Endoproteolytic processing of the lymphocytic choriomeningitis virus glycoprotein by the subtilase Ski-1/S1p. (springer.com)
  • Unusual molecular architecture of the machupo virus attachment glycoprotein. (springer.com)
  • These virions contain a single virus‑encoded membrane glycoprotein, GP, which is organized into homotrimeric spikes on the viral surface (see [ 7 , 8 , 9 ] for recent reviews). (mdpi.com)
  • 1953) and by Hilleman and Werner (1954) aroused great interest and excitement among clinicians and virologists alike in that no new acute viral respiratory disease of humans had been isolated since the identification of influenza virus 20 years earlier (Smith et al. (wikipedia.org)
  • But pets also carry certain bacteria, viruses, parasites, and fungi that can cause illness if transmitted to humans. (kidshealth.org)
  • Humans acquire LCM virus from infected rodents by coming in contact with the animals or their excretions. (thefreedictionary.com)
  • Influenza a(H3N2) virus in swine at agricultural fairs and transmission to Humans, Michigan and Ohio, USA, 2016. (thefreelibrary.com)
  • Nucleotide sequences of the H3N2v viruses detected in humans were deposited in the GISAID database (online Technical Appendix, https ://www.nc.cdc.gov/EID/article/23191170847-Techapp.pdf). (thefreelibrary.com)
  • The viruses recovered from swine were nearly identical to viruses identified in humans, and human virus gene segment sequences were nested within monophyletic swine virus clades. (thefreelibrary.com)
  • It has been previously reported that Lymphocytic Choriomeningitis nucleoprotein expressed in HEK cells (HEK-NP) could be cross presented to CD8+ T cells. (queensu.ca)
  • Lysosomotropic weak bases (chloroquine and ammonium chloride) and carboxylic ionophores (monensin and nigericin) inhibited virus entry, assessed as virus nucleoprotein expression at early times post-infection, indicating that the entry process involved a pH-dependent fusion step in intracellular vesicles. (ox.ac.uk)
  • Lymphocytic choriomeningitis virus (LCVM) nucleoprotein (NP) counteracts the host type I interferon (IFN) response by inhibiting activation of the IFN regulatory factor 3 (IRF3). (pubmedcentralcanada.ca)
  • Serologic test results were also negative for cytomegalovirus, rubella virus, Toxoplasma gondii , and Treponema pallidum . (cdc.gov)
  • Viral infections, particularly herpes simplex virus, varicella zoster virus, and cytomegalovirus, result either due to reactivation of latent viruses or new infections as sequelae of chemotherapy and debilitated cell-mediated immunity. (aacrjournals.org)
  • Infection with cytomegalovirus, Toxoplasma gondii , or LCM virus can appear similar enough in infants to be confused when diagnosed. (thefreedictionary.com)
  • Finally, virus control was found to be somewhat impaired in both mutant strains. (asm.org)
  • A quantal microassay for the titration of LCM virus strains is described. (microbiologyresearch.org)
  • I. Generation and recognition of virus strains and H-2b mutants. (semanticscholar.org)
  • Genomic and biological variation among commonly used lymphocytic choriomeningitis virus strains. (semanticscholar.org)
  • RNA sequence was similar to strains previously isolated offspring, that shed virus throughout life ( 2 ). (cdc.gov)
  • Zoonotic transmission allows for the introduction of novel influenza A virus strains to the human population, which has the potential to cause the next influenza pandemic. (thefreelibrary.com)
  • We examined the role of exhibition swine in the transmission of this reassortant influenza A virus, which contained a hemagglutinin gene from 2010-11 human seasonal H3N2 strains. (thefreelibrary.com)
  • Asnis DS , Muana O , Kim DG , Garcia M , Rollin PE , Slavinski S . Lymphocytic choriomeningitis virus meningitis, New York, NY, USA, 2009. (cdc.gov)
  • Lymphocytic for aseptic meningitis and encephalitis ( 6 ) and may cause congenital malformations or abortion ( 7 ). (cdc.gov)
  • Virus in Wood with aseptic meningitis ( 9 ). (cdc.gov)
  • Lymphocytic choriomeningitis, or LCM, is a rodent-borne viral infectious disease that presents as aseptic meningitis (inflammation of the membrane, or meninges, that surrounds the brain and spinal cord), encephalitis (inflammation of the brain), or meningoencephalitis (inflammation of both the brain and meninges). (news-medical.net)
  • It is caused by the lymphocytic choriomeningitis virus and characterized by fever, headache, and stiff neck often complicated by aseptic meningitis. (thefreedictionary.com)
  • Viruses are the most common cause of aseptic meningitis . (wikipedia.org)
  • [2] Most cases of viral meningitis are caused by enteroviruses (common stomach viruses). (wikipedia.org)
  • The viruses that cause meningitis are typically acquired from sick contacts. (wikipedia.org)
  • However, in most cases, people infected with viruses that may cause meningitis do not actually develop meningitis. (wikipedia.org)
  • Selection of genetic variants of lymphocytic choriomeningitis virus in spleens of persistently infected mice. (springer.com)
  • Elevated natural killer cell-mediated cytotoxicity, plasma interferon, and tumor cell rejection in mice persistently infected with lymphocytic choriomeningitis virus. (jimmunol.org)
  • Surviving mice became persistently infected carriers, with high virus titers in blood and brain. (rupress.org)
  • Lymphocytic choriomeningitis (LCM) is a rare viral disease that can be transmitted through the urine, droppings, saliva, or cage material of infected wild and domestic rodents, including hamsters, guinea pigs, rats, mice, and other small rodents. (cdc.gov)
  • Pet rodents and fatal lymphocytic choriomeningitis in transplant patients. (labome.org)
  • Presents information on lymphocytic choriomeningitis virus, an arenavirus primarily affecting rodents. (ebscohost.com)
  • Food and dust can become contaminated by the excretions of rodents infected with LCM virus. (thefreedictionary.com)
  • Congenital lymphocytic choriomeningitis virus: an unrecognized teratogenic pathogen. (cdc.gov)
  • Congenital Lymphocytic Choriomeningitis Virus Infection: Decade of Rediscovery. (ebscohost.com)
  • Analysis of acute influenza virus infections uncovered the prominent direct role that cytokine storm plays in the pathogenesis, morbidity, and mortality from this disease. (pnas.org)
  • Thus, studies in Th-deficient mice revealed a complete dependence on CD4 + T cells for the acute and long-term control of infection with viruses normally causing chronic infections ( 1 , 2 ). (jimmunol.org)
  • In contrast, transient virus control may be observed following infection of CD4 + T cell-deficient mice with viruses, which in immunocompetent mice causes only acute and self-limiting infection ( 3 ). (jimmunol.org)
  • In contrast, we recently demonstrated significant acute virus control and secondary expansion in Th-deficient mice vaccinated 60 d previously with an adenovirus-vectored Ag engineered for enhanced presentation ( 8 ), but the question of chronic virus control was not addressed. (jimmunol.org)
  • More importantly, EM011 treatment at tumor-suppressive dosages (300 mg/kg) does not suppress cell-mediated immune responses in mice experimentally challenged with acute lymphocytic choriomeningitis virus infection, in that mice mount robust virus-specific CD8 + and CD4 + T-cell immune responses while maintained on daily drug treatment. (aacrjournals.org)
  • Acute infection of adult mice leads to generalized virus spread, followed by cytotoxic T lymphocyte-mediated virus clearance below the detection levels of conventional assays within 2-3 weeks. (ox.ac.uk)
  • Here, we evaluated the in vivo effects of IM156 on antigen-specific CD8⁺ T cells during their effector and memory differentiation after acute lymphocytic choriomeningitis virus infection. (bvsalud.org)
  • Measles virus most often causes an acute infection accompanied by fevers and characteristic rashes. (scripps.edu)
  • To determine the efficacy of replication-defective lymphocytic choriomeningitis virus (rLCMV) vectors expressing the guinea pig CMV (GPCMV) antigens, gB and pp65, in the guinea pig model of congenital CMV infection. (nih.gov)
  • Unlike what occurred with the original rLCMV/LASV wild-type (wt) GP, infection of C57BL/6 mice with the mutated recombinant virus led to a detectable viremia of 2 weeks' duration. (nih.gov)
  • We undertook evaluation of a novel bivalent vaccine based on nonreplicating lymphocytic choriomeningitis virus (rLCMV) vectors expressing a cytoplasmic tail-deleted gB [gB(dCt)] and full-length pp65 from human CMV in mice. (nih.gov)
  • Replication-incompetent lymphocytic choriomeningitis virus (rLCMV) vectors do not elicit vector-neutralizing antibody responses, and homologous prime-boost regimens with rLCMV vectors induce boostable and protective T cell responses to model antigens in mice. (jpt.com)
  • Replication of these GP-pseudotyped rLCMVΔGP/GFP viruses was restricted to GP-expressing cell lines. (scripps.edu)
  • Kamperschroer, C & Quinn, D 2002, ' The role of proinflammatory cytokines in wasting disease during lymphocytic choriomeningitis virus infection ', JOURNAL OF IMMUNOLOGY , vol. 169, no. 1, pp. 340-349. (ulster.ac.uk)
  • Because of its role in protein synthesis, it is an essential molecule for the survival of the normal cell as well as for synthesis of new viral proteins in a virus-infected cell. (rupress.org)
  • Mutations that functionally impair these viral IFN-counteracting factors often cause attenuation both in vitro and in vivo, as illustrated by influenza and Ebola viruses containing mutant NS1 and VP35, respectively, proteins unable to inhibit IRF3 activation ( 7 , 15 , 17 , 18 , 20 , 43 ). (pubmedcentralcanada.ca)
  • These proteins were characterized with a virus overlay protein blot assay. (ox.ac.uk)
  • Expression levels of certain proteins can affect the immune system's ability to neutralize a virus. (news-medical.net)
  • The virologists also integrated certain proteins into the virus that are otherwise found only in cancer cells. (eurekalert.org)
  • Infection with the designer virus enabled the immune system to recognize these cancer proteins as dangerous. (eurekalert.org)
  • Lymphocytic choriomeningitis (LCM) virus interfering particles were enriched relative to infectious virions by ultracentrifugation in a shallow gradient made of Urografin. (microbiologyresearch.org)
  • Alanine substitutions at several positions within this region resulted in NP mutants that lacked the IFN-counteracting activity but retained their functions in virus RNA synthesis and assembly of infectious particles. (pubmedcentralcanada.ca)
  • NIH investigators and colleagues have discovered that when the immune system first responds to infectious agents such as viruses or bacteria, a natural brake on the response prevents overactivation. (news-medical.net)
  • Infectious T lymphocytes were determined in thymuses and spleens of mice acutely infected with LCM virus. (ias.ac.in)
  • Either susceptibility to LCM virus is associated with some degree of immaturity or the probability of murine T lymphocytes to encounter the virus as an infectious entity rather than an immunogen is higher in very young than immunologically mature mice. (ias.ac.in)
  • Further, they provide new problems for healthcare persons owing to the narrowing of the world as visitors from many countries travel increasingly to and from endemic areas and may incubate the infectious agent taking it from an endemic area into an area where the virus is not expected. (springer.com)
  • Dissection of antiviral and immune regulatory functions of tumor necrosis factor receptors in a chronic lymphocytic choriomeningitis virus infection. (semanticscholar.org)
  • Toll-like receptors (TLRs), the retinoic acid-inducible gene I (RIG-I)-like helicases (RLHs), RIG-I, and melanoma differentiation-associated gene 5 (MDA5) will be the main pattern identification receptors (PRR) of pet cells spotting RNA viruses through the early stage of infections (2, 45, 57). (saracatinib.info)
  • Several years ago, using transgenic techniques he developed the first in vivo models that carry the measles receptors and are susceptible to the virus. (scripps.edu)
  • A Cluster of Lymphocytic Choriomeningitis Virus Infections Transmitted Through Organ Transplantation - Iowa, 2013. (medscape.com)
  • At present, the only feasible way to prevent further cases is the physician's special attention to the possibility of rodent contacts of patients with CNS disease and early laboratory confirmation of suspected cases of human LCM virus infections. (unboundmedicine.com)
  • Results of PCR screening of amniotic fluid were negative for TORCH agents ( t oxoplasmosis/ Toxoplasma gondii , o ther infections, r ubella virus, c ytomegalovirus, h erpes simplex virus-2 or neonatal herpes simplex virus), as well as enterovirus, Listeria monocytogenes , Mycoplasma spp. (cdc.gov)
  • Early during viral infections, innate immunity is essential to restrict viral replication and systemic spreading until the virus-specific adaptive immune response can effectively eliminate the virus. (asm.org)
  • Immunocompromisation is a major cause of opportunistic infections, activation of latent viruses, and the development of certain carcinomas such as Kaposi's sarcoma ( 1 , 2 ). (aacrjournals.org)
  • Lymphocytic Choriomeningitis Virus Infections among American Indians. (ebscohost.com)
  • these unique swine-human interfaces have generated most human infections with variant influenza A virus in the United States (1). (thefreelibrary.com)
  • During July-August 2016, outbreaks of variant H3N2 virus (H3N2v) were reported in Ohio and Michigan, and 18 zoonotic influenza A virus infections were detected (2). (thefreelibrary.com)
  • The manifestations of the disease are thought to result from the host response to the virus as natural killer cells and cytotoxic T cells respond to infection with the production of interferon and additional inflammatory mediators. (medscape.com)
  • These data indicate that virus specific memory CD8 T cells can rapidly acquire cytotoxic function upon re-exposure to Ag and are much more efficient killers in vivo than previously appreciated. (labome.org)
  • Infection of lymphocytes by a virus that aborts cytotoxic T lymphocyte activity and establishes persistent infection. (springer.com)
  • Antivirally protective cytotoxic T cell memory to lymphocytic choriomeningitis virus is governed by persisting antigen. (curehunter.com)
  • In such mice, the generation of virus-specific cytotoxic T lymphocytes was only slightly impaired and bystander activation was as extensive as that observed in wild-type mice. (asm.org)
  • Biology of cloned cytotoxic T lymphocytes specific for lymphocytic choriomeningitis virus. (semanticscholar.org)
  • Expression of cDNA copies of these genes in vaccinia virus vectors demonstrates that C57BL/6 (H2bb) mice mount significant CTL responses to both GP and NP. (scripps.edu)
  • Development of replication-defective lymphocytic choriomeningitis virus vectors for the induction of potent CD8+ T cell immunity. (unil.ch)
  • Hence, receptor-virus interaction on DCs in vivo is an essential step in the initiation of virus-induced immunosuppression and viral persistence. (springer.com)
  • Persistence of lymphocytic choriomeningitis virus at very low levels in immune mice. (ox.ac.uk)
  • Additionally the GP260 mutation which is responsible for improved a-dystroglycan affinity and DC focusing on played an accessory role B-HT 920 2HCl in enhancing the period of persistence and generalized immunosuppression by those viruses that also carried the L1079 position of Cl13. (lecollege.org)
  • Otherwise the carried virus resembled the original armstrong virus in conferring solid immunity to mice, reacting specifically with antibodies and sedimenting in the ultracentrifuge like lymphocytic choriomeningitis virus. (microbiologyresearch.org)
  • Treatment of L (Arm) cultures with lymphocytic choriomeningitis-neutralizing antibodies slowly decreased the proportion of antigen-containing cells. (microbiologyresearch.org)
  • A similar mechanism may be important in the chronic autoimmune-like disease in virus carrier mice 2,5 . (nature.com)
  • Investigation into the mechanism of how virus-infected DCs cause immunosuppression reveals loss of MHC class II surface expression and costimulatory molecules on surface of such DCs. (springer.com)
  • Because several other viruses known to cause immunosuppression (HIV, measles) interact with DCs, the observations noted here are likely a common selective mechanism by which viruses also are able to evade the host's immune system. (springer.com)
  • Mechanism of lymphocytic choriomeningitis virus entry into cells. (ox.ac.uk)
  • He looks at the mechanism, asking how the virus interacts with the host and how the host interacts with the virus. (scripps.edu)
  • Unexpectedly, our results showed that in vivo treatment of IM156 exacerbated the memory differentiation of virus-specific CD8⁺ T cells, resulting in an increase in short-lived effector cells but decrease in memory precursor effector cells. (bvsalud.org)
  • Others and we have previously described the potent in vivo and in vitro activity of the broad-spectrum antiviral agent PAP (Pokeweed antiviral protein) against a wide range of viruses. (springer.com)
  • Likewise, several New World arenaviruses, chiefly Junin virus, cause viral HF disease ( 3 ). (pnas.org)
  • Recent studies have identified small molecule inhibitors of Tacaribe virus ( 7 ) and LASV ( 8 ) cell entry, but their antiviral activity in animal models of HF arenaviruses has not been examined. (pnas.org)
  • Arenaviruses are enveloped viruses with a bisegmented negative-strand (NS) RNA genome ( 9 ). (pnas.org)
  • The investigation of antiviral strategies to combat HF arenaviruses is hampered by the requirement of biosafety level 4 (BSL-4) facilities to work with these viruses. (scripps.edu)
  • LCM is caused by an arenavirus, which is an RNA virus and is a mild cousin in the family containing the much more threatening arenaviruses that cause hemmorrhagic fever . (thefreedictionary.com)
  • The arenaviruses are a large family of emerging negative-stranded RNA viruses that include several severe human pathogens causing hemorrhagic fevers with high mortality. (springer.com)
  • This observation indicated that the persistent infection of L cells with the lymphocytic choriomeningitis virus, strain armstrong , was not only maintained by a vertical transfer of virus from parent to daughter cells but that horizontal spread played a role. (microbiologyresearch.org)
  • The Clone 13 (Cl13) strain of lymphocytic choriomeningitis virus is widely studied as a style of chronic systemic viral infection. (lecollege.org)
  • In a similar way, CTLs specific for a vesicular stomatitis virus (VSV) peptide were generated by immunizing mice with another hsp, gp96, purified from VSV-infected cells. (rupress.org)
  • Labudová M, Pastorek J, Pastoreková S. Lymphocytic choriomeningitis virus: ways to establish and maintain non-cytolytic persistent infection. (medscape.com)
  • These findings reveal a novel role for antigen in maintaining virus-specific CD8 T cells during persistent infection and provide insight toward understanding T cell differentiation in chronic infection. (labome.org)
  • Persistent infection of some standard cell lines by lymphocytic choriomeningitis virus. (uu.nl)
  • Persistent infection of mice with the virus of lymphocytic choriomeningitis: virus-specific immunological tolerance. (semanticscholar.org)
  • These results show that a single change in the genome can affect viral pathogenicity by altering the tropism of the virus. (jenner.ac.uk)
  • This is a list of representative viruses (grouped by genome structure) compiled from: 'Virus Taxonomy', the Sixth Report of the International Committee on Taxonomy of Viruses (ICTV) 1995. (virology.net)
  • The fulminating disease, which occurs in adult mice after intracerebral inoculation with LCM virus, can be ameliorated by several treatments known to impair the immune response of the host, such as X-rays 6 , amethopterin 7 , thymectomy 8 and anti-lymphocyte serum 9 . (nature.com)
  • A single dose of 150 mg/g of cyclophosphamide (CY), given 3 days after intracerebral (i.c.) inoculation of lymphocytic choriomeningitis (LCM) virus, protected over 90% of adult BALB/c mice against acutely fatal choriomeningitis. (rupress.org)
  • Epitope specificity and relative clonal abundance do not affect CD8 differentiation patterns during lymphocytic choriomeningitis virus infection. (ox.ac.uk)
  • For instance, West Nile virus , mumps , measles , herpes simplex types I and II, varicella and lymphocytic choriomeningitis (LCM) virus. (wikipedia.org)
  • The patient had been vaccinated against varicella virus. (cdc.gov)
  • The vesicular stomatitis virus (VSV) has proven to be a potent viral vector vaccine candidate.However, translation of these promising studies into clinical application has so far been limited by VSVs neurotropism. (uibk.ac.at)
  • Mice were treated either with vehicle or PAP administered intraperitoneally 24 hours prior to, 1 hour prior to and 24 hours, 48 hours 72 hours and 96 hours after virus inoculation. (springer.com)
  • The pulmonary localization of virus-specific T lymphocytes is governed by the tissue tropism of infection. (semanticscholar.org)
  • Lehmann-Grube, F. (1980) Infection of murine T lymphocytes with lymphocytic choriomeningitis virus: effect of age of mice on susceptibility Zeitschrift für Naturforschung - C, 35 (11-12). (ias.ac.in)
  • Development of a reverse transcription-polymerase chain reaction assay for diagnosis of lymphocytic choriomeningitis virus infection and its use in a prospective surveillance study. (uab.edu)
  • A species of FLAVIVIRUS, one of the Japanese encephalitis virus group (ENCEPHALITIS VIRUSES, JAPANESE). (nih.gov)
  • heterologous interference against vesicular stomatitis, encephalomyocarditis and vaccinia viruses could not be demonstrated. (microbiologyresearch.org)
  • Infection in man is acquired after inhalation, ingestion or direct contact with virus found in the urine, feces and saliva of infected mice, hamsters and guinea pigs. (springer.com)
  • Cytokine storm of influenza virus infection is initiated via a pulmonary endothelial cell amplification loop involving IFN-producing cells and virus-infected pulmonary epithelial cells. (pnas.org)
  • In accordance with this hypothesis, an A-to-G/U-to-C hypermutation pattern has been observed in several negative-strand RNA viruses ( 9 , 25 , 30 , 41 ). (asm.org)
  • Members of the family Filoviridae (filoviruses) are non-segmented negative-strand RNA viruses that produce filamentous enveloped virions. (mdpi.com)
  • These recombinant chimeric viruses could allow the study of immune responses and antivirals targeting the LASV GP. (nih.gov)
  • In addition, evidence indicates that morbidity and mortality associated with LASV infection involve a failure of the host's innate immune response to restrict virus replication and to facilitate the initiation of an effective adaptive immune response ( 31 ). (pubmedcentralcanada.ca)
  • Gram stain and culture for bacterial pathogens and herpes simplex virus-1/2 polymerase chain reaction (PCR) were negative in CSF. (cdc.gov)
  • The L segment encodes the viral RNA-dependent RNA polymerase (or L polymerase), and a small (≈12 kDa) RING finger protein Z that is functionally the counterpart of the matrix protein found in many enveloped NS RNA viruses. (pnas.org)