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Aequorin
A photoprotein isolated from the bioluminescent jellyfish Aequorea. It emits visible light by an intramolecular reaction when a trace amount of calcium ion is added. The light-emitting moiety in the bioluminescence reaction is believed to be 2-amino-3-benzyl-5-(p-hydroxyphenyl)pyrazine (AF-350).
Luminescent Measurements
Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.
Luminescent Proteins
Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.
Green Fluorescent Proteins
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
Internet
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
Databases, Protein
Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.
User-Computer Interface
The portion of an interactive computer program that issues messages to and receives commands from a user.
Software
Sequential operating programs and data which instruct the functioning of a digital computer.
Proteins
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.

Mitochondrial depolarization accompanies cytochrome c release during apoptosis in PC6 cells. (1/8843)

Cytochrome c is released from mitochondria into the cytosol in cells undergoing apoptosis. The temporal relationship between cytochrome c release and loss of mitochondrial membrane potential was monitored by laser-scanning confocal microscopy in single living pheochromocytoma-6 cells undergoing apoptosis induced by staurosporine. Mitochondrial membrane potential monitored by tetramethylrhodamine methyl ester decreased abruptly in individual cells from 2 to 7 h after treatment with staurosporine. Depolarization was accompanied by cytochrome c release documented by release of transfected green fluorescent protein-tagged cytochrome c in these cells. The results show that mitochondrial depolarization accompanies cytochrome c release in pheochromocytoma-6 cells undergoing apoptosis.  (+info)

The dually acylated NH2-terminal domain of gi1alpha is sufficient to target a green fluorescent protein reporter to caveolin-enriched plasma membrane domains. Palmitoylation of caveolin-1 is required for the recognition of dually acylated g-protein alpha subunits in vivo. (2/8843)

Here we investigate the molecular mechanisms that govern the targeting of G-protein alpha subunits to the plasma membrane. For this purpose, we used Gi1alpha as a model dually acylated G-protein. We fused full-length Gi1alpha or its extreme NH2-terminal domain (residues 1-32 or 1-122) to green fluorescent protein (GFP) and analyzed the subcellular localization of these fusion proteins. We show that the first 32 amino acids of Gi1alpha are sufficient to target GFP to caveolin-enriched domains of the plasma membrane in vivo, as demonstrated by co-fractionation and co-immunoprecipitation with caveolin-1. Interestingly, when dual acylation of this 32-amino acid domain was blocked by specific point mutations (G2A or C3S), the resulting GFP fusion proteins were localized to the cytoplasm and excluded from caveolin-rich regions. The myristoylated but nonpalmitoylated (C3S) chimera only partially partitioned into caveolin-containing fractions. However, both nonacylated GFP fusions (G2A and C3S) no longer co-immunoprecipitated with caveolin-1. Taken together, these results indicate that lipid modification of the NH2-terminal of Gi1alpha is essential for targeting to its correct destination and interaction with caveolin-1. Also, a caveolin-1 mutant lacking all three palmitoylation sites (C133S, C143S, and C156S) was unable to co-immunoprecipitate these dually acylated GFP-G-protein fusions. Thus, dual acylation of the NH2-terminal domain of Gi1alpha and palmitoylation of caveolin-1 are both required to stabilize and perhaps regulate this reciprocal interaction at the plasma membrane in vivo. Our results provide the first demonstration of a functional role for caveolin-1 palmitoylation in its interaction with signaling molecules.  (+info)

Properties of filament-bound myosin light chain kinase. (3/8843)

Myosin light chain kinase binds to actin-containing filaments from cells with a greater affinity than to F-actin. However, it is not known if this binding in cells is regulated by Ca2+/calmodulin as it is with F-actin. Therefore, the binding properties of the kinase to stress fibers were examined in smooth muscle-derived A7r5 cells. Full-length myosin light chain kinase or a truncation mutant lacking residues 2-142 was expressed as chimeras containing green fluorescent protein at the C terminus. In intact cells, the full-length kinase bound to stress fibers, whereas the truncated kinase showed diffuse fluorescence in the cytoplasm. After permeabilization with saponin, the fluorescence from the truncated kinase disappeared, whereas the fluorescence of the full-length kinase was retained on stress fibers. Measurements of fluorescence intensities and fluorescence recovery after photobleaching of the full-length myosin light chain kinase in saponin-permeable cells showed that Ca2+/calmodulin did not dissociate the kinase from these filaments. However, the filament-bound kinase was sufficient for Ca2+-dependent phosphorylation of myosin regulatory light chain and contraction of stress fibers. Thus, dissociation of myosin light chain kinase from actin-containing thin filaments is not necessary for phosphorylation of myosin light chain in thick filaments. We note that the distance between the N terminus and the catalytic core of the kinase is sufficient to span the distance between thin and thick filaments.  (+info)

A fluorescent orthotopic bone metastasis model of human prostate cancer. (4/8843)

Here, we report a fluorescent spontaneous bone metastatic model of human prostate cancer developed by surgical orthotopic implantation of green fluorescent protein (GFP)-expressing prostate cancer tissue. Human prostate cancer PC-3 cells were transduced with the pLEIN expression retroviral vector containing the enhanced GFP and neomycin resistance genes. Stable GFP high-expression PC-3 clones were selected in vitro with G418, which were then combined and injected s.c. in nude mice. For metastasis studies, fragments of a single highly fluorescent s.c. growing tumor were implanted by surgical orthotopic implantation in the prostate of a series of nude mice. Subsequent micrometastases and metastases were visualized by GFP fluorescence throughout the skeleton, including the skull, rib, pelvis, femur, and tibia The central nervous system, including the brain and spinal cord, was also involved with tumor, as visualized by GFP fluorescence. Systemic organs, including the lung, plural membrane, liver, kidney, and adrenal gland, also had fluorescent metastases. The metastasis pattern in this model reflects the bone and other metastatic sites of human prostate cancer. Thus, this model should be very useful for the study and development of treatment for metastatic androgen-independent prostate cancer.  (+info)

Role of cytokine signaling molecules in erythroid differentiation of mouse fetal liver hematopoietic cells: functional analysis of signaling molecules by retrovirus-mediated expression. (5/8843)

Erythropoietin (EPO) and its cell surface receptor (EPOR) play a central role in proliferation, differentiation, and survival of erythroid progenitors. Signals induced by EPO have been studied extensively by using erythroid as well as nonerythroid cell lines, and various controversial results have been reported as to the role of signaling molecules in erythroid differentiation. Here we describe a novel approach to analyze the EPO signaling by using primary mouse fetal liver hematopoietic cells to avoid possible artifacts due to established cell lines. Our strategy is based on high-titer retrovirus vectors with a bicistronic expression system consisting of an internal ribosome entry site (IRES) and green fluorescent protein (GFP). By placing the cDNA for a signaling molecule in front of IRES-GFP, virus-infected cells can be viably sorted by fluorescence-activated cell sorter, and the effect of expression of the signaling molecule can be assessed. By using this system, expression of cell-survival genes such as Bcl-2 and Bcl-XL was found to enhance erythroid colony formation from colony-forming unit-erythroid (CFU-E) in response to EPO. However, their expression was not sufficient for erythroid colony formation from CFU-E alone, indicating that EPO induces signals for erythroid differentiation. To examine the role of EPOR tyrosine residues in erythroid differentiation, we introduced a chimeric EGFR-EPOR receptor, which has the extracellular domain of the EGF receptor and the intracellular domain of the EPOR, as well as a mutant EGFR-EPOR in which all the cytoplasmic tyrosine residues are replaced with phenylalanine, and found that tyrosine residues of EPOR are essential for erythroid colony formation from CFU-E. We further analyzed the function of the downstream signaling molecules by expressing modified signaling molecules and found that both JAK2/STAT5 and Ras, two major signaling pathways activated by EPOR, are involved in full erythroid differentiation.  (+info)

Nuclear translocation of green fluorescent protein-nuclear factor kappaB with a distinct lag time in living cells. (6/8843)

A highly fluorescent mutant form of the green fluorescent protein (GFP) has been fused to the human nuclear factor kappaB (NF-kappaB) p50 and p105 (p50/IkappaB gamma), a precursor protein of NF-kappaB p50. GFP-p50 and GFP-p105 were expressed in monkey COS-7 cells and human HeLa cells. Translocation of these chimeric proteins was observed by confocal laser scanning microscopy. GFP-p50 (without IkappaB gamma) in the transfected cells resided in the nucleus. On the other hand, GFP-p105 (GFP-p50 with IkappaB gamma) localized only in the cytoplasm before stimulation and translocated to the nucleus with stimulant specificity similar to that of native NF-kappaB/IkappaB. In addition, the translocation of NF-kappaB to the nucleus had a distinct lag time (a quiescent time) in the target cells. The lag time lasted 10-20 min after stimulation with hydrogen peroxide or tumor necrosis factor alpha. It was suggested that this might be due to the existence of a limiting step where NF-kappaB is released from NF-kappaB/IkappaB by the proteasome.  (+info)

Microtubule-dependent plus- and minus end-directed motilities are competing processes for nuclear targeting of adenovirus. (7/8843)

Adenovirus (Ad) enters target cells by receptor-mediated endocytosis, escapes to the cytosol, and then delivers its DNA genome into the nucleus. Here we analyzed the trafficking of fluorophore-tagged viruses in HeLa and TC7 cells by time-lapse microscopy. Our results show that native or taxol-stabilized microtubules (MTs) support alternating minus- and plus end-directed movements of cytosolic virus with elementary speeds up to 2.6 micrometer/s. No directed movement was observed in nocodazole-treated cells. Switching between plus- and minus end-directed elementary speeds at frequencies up to 1 Hz was observed in the periphery and near the MT organizing center (MTOC) after recovery from nocodazole treatment. MT-dependent motilities allowed virus accumulation near the MTOC at population speeds of 1-10 micrometer/min, depending on the cell type. Overexpression of p50/dynamitin, which is known to affect dynein-dependent minus end-directed vesicular transport, significantly reduced the extent and the frequency of minus end-directed migration of cytosolic virus, and increased the frequency, but not the extent of plus end-directed motility. The data imply that a single cytosolic Ad particle engages with two types of MT-dependent motor activities, the minus end- directed cytoplasmic dynein and an unknown plus end- directed activity.  (+info)

Estimation of the number of alpha-helical and beta-strand segments in proteins using circular dichroism spectroscopy. (8/8843)

A simple approach to estimate the number of alpha-helical and beta-strand segments from protein circular dichroism spectra is described. The alpha-helix and beta-sheet conformations in globular protein structures, assigned by DSSP and STRIDE algorithms, were divided into regular and distorted fractions by considering a certain number of terminal residues in a given alpha-helix or beta-strand segment to be distorted. The resulting secondary structure fractions for 29 reference proteins were used in the analyses of circular dichroism spectra by the SELCON method. From the performance indices of the analyses, we determined that, on an average, four residues per alpha-helix and two residues per beta-strand may be considered distorted in proteins. The number of alpha-helical and beta-strand segments and their average length in a given protein were estimated from the fraction of distorted alpha-helix and beta-strand conformations determined from the analysis of circular dichroism spectra. The statistical test for the reference protein set shows the high reliability of such a classification of protein secondary structure. The method was used to analyze the circular dichroism spectra of four additional proteins and the predicted structural characteristics agree with the crystal structure data.  (+info)

citrine crystal propertiescitrine crystal properties
Citrine crystal meaning is based on the yellow hues in citrine color. And even though yellow stone tends to yield more masculine than feminine quality, anyone can get great benefits healing power from Citrine stone . Often used for chronic fatigue, Citrine has a reputation of being able to increase energy and drive. Citrine is a powerful cleanser and regenerator. Children: It helps children - including teenagers - to feel loved. The Properties Of Citrine Citrine crystals are usually yellow, yellow-brown, orange, dark orange-brown, and reddish-brown in color. Citrine crystals, clusters, and geodes. Via the third eye chakra this natural form of Citrine allows for the clarity of mind needed to aid in manifestation work. The citrine crystal resembles that of the topaz, and its color is due to chemical impurities. As noted, citrine is formed by heat-treating amethyst, specifically purple amethyst. Citrine is one of the crystals that never needs cleaning. Citrine is a stone of abundance and optimism. ...
https://customerlabs.co/g4i2n/kr2xb7.php?id=citrine-crystal-properties-8d33cc
Resonance CARS Study of the Structure of Green and Red Chromophores within the Red Fluorescent Protein DsRedResonance CARS Study of the Structure of Green and Red Chromophores within the Red Fluorescent Protein DsRed
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https://www.infona.pl/resource/bwmeta1.element.acs-doi-10_1021_ja0260824
A monomeric red fluorescent protein | PNASA monomeric red fluorescent protein | PNAS
Initially, we were faced with the decision of how to efficiently construct and screen libraries of DsRed mutants such that we could direct the evolution of tetrameric DsRed toward a mRFP. Ultimately the best solution was to take the semirational approach of breaking the dimer interfaces in a stepwise fashion (first AB then AC) and undertaking a directed evolution strategy to rescue the red fluorescence. This combination of targeted and random mutagenesis successfully directed the evolution of DsRed from the poorly fluorescent dimer T1-I125R to the monomeric mRFP1 in eight generations. In retrospect, breaking up the tetramer was not the barrier to the discovery of mRFP1; the challenge was to find the correct combination of many mutations to rescue the red fluorescence in the crippled dimers and monomers.. There are likely several mechanisms by which mutations in dimer2 and mRFP1 have contributed to rescuing the red fluorescence. Although one mechanism probably involves improving the folding ...
https://www.pnas.org/content/99/12/7877?ijkey=47f7ad3c6b7cc70dc4eb2b7e2350fc40b1ada6e7&keytype2=tf_ipsecsha
A monomeric red fluorescent protein | PNASA monomeric red fluorescent protein | PNAS
Initially, we were faced with the decision of how to efficiently construct and screen libraries of DsRed mutants such that we could direct the evolution of tetrameric DsRed toward a mRFP. Ultimately the best solution was to take the semirational approach of breaking the dimer interfaces in a stepwise fashion (first AB then AC) and undertaking a directed evolution strategy to rescue the red fluorescence. This combination of targeted and random mutagenesis successfully directed the evolution of DsRed from the poorly fluorescent dimer T1-I125R to the monomeric mRFP1 in eight generations. In retrospect, breaking up the tetramer was not the barrier to the discovery of mRFP1; the challenge was to find the correct combination of many mutations to rescue the red fluorescence in the crippled dimers and monomers.. There are likely several mechanisms by which mutations in dimer2 and mRFP1 have contributed to rescuing the red fluorescence. Although one mechanism probably involves improving the folding ...
https://www.pnas.org/content/99/12/7877?ijkey=273839a4cf3c2b5d1d41247c50dc4df0b873f099&keytype2=tf_ipsecsha
Revealing the Excited-State Dynamics of the Fluorescent Protein Dendra2
Green-to-red photoconversion is a reaction that occurs in a limited number of fluorescent proteins and that is currently mechanistically debated. In this contribution, we report on our investigation of the photoconvertible fluorescent protein Dendra2 by employing a combination of pump-probe, up-conversion and single photon timing spectroscopic techniques. Our findings indicate that upon excitation of the neutral green state an excited state proton transfer proceeds with a time constant of 3.4 ps between the neutral green and the anionic green states. In concentrated solution we detected resonance energy transfer (25 ps time constant) between green and red monomers. The time-resolved emission spectra suggest also the formation of a super-red species, first observed for DsRed (a red fluorescent protein from the corallimorph species Discosoma) and consistent with peculiar structural details present in both proteins.
http://hal.univ-grenoble-alpes.fr/hal-01662511
Engineering novel fluorescent proteinsEngineering novel fluorescent proteins
Author(s): Shaner, Nathan Christopher | Abstract: Fluorescent proteins are intrinsically fluorescent, genetically encodable tags that can be expressed in many heterologous organisms. Originally cloned from jellyfish and corals, these proteins and their engineered derivatives have become ubiquitous tools in molecular and cell biology. While wild-type fluorescent proteins sometimes possess sufficiently beneficial properties to be used unmodified, many applications require improvements in brightness or photostability, reduction of oligomerization, or other specific properties that require additional engineering of the wild-type protein. This dissertation presents experiments drawn from the entire spectrum of fluorescent protein science, from the cloning of novel wild-type fluorescent proteins to the engineering of wavelength-shifted, photostable, and photoactivatable variants of existing fluorescent proteins. The previously engineered monomeric red fluorescent protein, mRFP1, was engineered through a
https://escholarship.org/uc/item/3kx7d1fb
Citrine Informatics Raises $20M in Series B Funding - Citrine InformaticsCitrine Informatics Raises $20M in Series B Funding - Citrine Informatics
REDWOOD CITY, Calif.-(BUSINESS WIRE)-Citrine Informatics, the developer of the first materials artificial intelligence (AI) operating platform, announced today $20M in Series B funding. The round was co-led by Prelude Ventures and Innovation Endeavors with participation from Moore Strategic Ventures, Next47, and other investors. The financing will help support commercial growth of the Citrine Platform, the companys core product, as well as the expansion in business development and sales efforts in the United States, Europe, and Asia.. The technological innovation behind the Citrine Platform dramatically reduces the time and cost of materials and chemicals development by combining materials-specific data handling and storage, scalable compute for AI and data processing, along with modular composable AI units optimized to predict materials behavior. The Citrine Platform has helped some of the worlds largest organizations, including chemicals and product companies, hit overall R&D milestones in ...
https://citrine.io/media-post/citrine-informatics-raises-20m-in-series-b-funding/
pSAT vectors: a modular series of plasmids for autofluorescent protein tagging and expression of multiple genes in plants,...pSAT vectors: a modular series of plasmids for autofluorescent protein tagging and expression of multiple genes in plants,...
Read pSAT vectors: a modular series of plasmids for autofluorescent protein tagging and expression of multiple genes in plants, Plant Molecular Biology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
https://www.deepdyve.com/lp/springer_journal/psat-vectors-a-modular-series-of-plasmids-for-autofluorescent-protein-071cWHw45o
Red Fluorescent Proteins- mCherry, tdTomato, DsRed, AsRed & mStrawberryRed Fluorescent Proteins- mCherry, tdTomato, DsRed, AsRed & mStrawberry
Red Fluorescent Proteins- mCherry, tdTomato, DsRed, AsRed & mStrawberryRed Fluorescent Proteins- mCherry, tdTomato, DsRed, AsRed & mStrawberry
Far Red Fluorescent Proteins Far Red Fluorescent Proteins Red FluorescentFar Red Fluorescent Proteins Far Red Fluorescent Proteins Red Fluorescent
mKate2 is the next generation of monomeric far-red fluorescent protein TagFP635 (mKate) [Shcherbo et al., 2007; Shcherbo et al., 2009]. Possessing fluorescence with excitation/emission maxima at 588 and 633 nm, mKate2 is almost 3-fold brighter than TagFP635 and is 10-fold brighter than mPlum at the physiological pH = 7.5. Within the optical window optimal for light penetration in living tissues, calculated brightness of mKate2 is at least 2-fold higher compared to any monomeric fluorescent protein reported to date. mKate2 is characterized by complete and fast chromophore maturation at 37°C with maturation half-time ,20 min (versus 40 min for mCherry). It is more photostable under both widefield and confocal illumination than other monomeric far-red proteins, including TagFP635, mRaspberry and mPlum. The high brightness, far-red emission spectrum, excellent pH resistance and photostability, coupled with low toxicity demonstrated in transgenic Xenopus laevis embryos, make mKate2 a superior ...
https://capitalbiosciences.com/shop/category/show/mkate2.html
Towers - Natural Citrine Tower - Dark Extra (Brazil)- Citrine - Healing CrystalsTowers - Natural Citrine Tower - Dark Extra (Brazil)- Citrine - Healing Crystals
Natural Citrine Towers (Brazil) - These beautiful cut and polished Dark Extra grade Natural Citrine Towers from Brazil are made of natural (non-heat-treated) Citrine. These crystals are sold individually unless specified as a set and are pictured in multiples to demonstrate the range of
http://www.healingcrystals.com/Towers_-_Natural_Citrine_Tower_-_Dark__Extra___Brazil_.html
Gem, citrine (heated), 3mm faceted round, A grade, Mohs hardness 7. Sold per pkg of 6. - Fire Mountain Gems and BeadsGem, citrine (heated), 3mm faceted round, A grade, Mohs hardness 7. Sold per pkg of 6. - Fire Mountain Gems and Beads
Citrine ... the stone of wealth. Premium grade citrine varies from deep yellow to a wonderful yellow-orange hue. Very bright with minimal brown tones. Clarity is eye clean. Fine quality cutting. Almost all citrine today is created by heat-treating amethyst.
https://www.firemountaingems.com/itemdetails/h201765fg
IC-87114 novel inhibtior | Role of NK1 and NK2 receptors in mouse gastric mechanical activityIC-87114 novel inhibtior | Role of NK1 and NK2 receptors in mouse gastric mechanical activity
Supplementary MaterialsSupplementary Details Supplementary information srep00688-s1. results in the conformational dynamics from the RFP chromophore. The genetically-encoded fluorescent proteins (FPs) are effective equipment for imaging in biology1. The prototypical green fluorescent proteins (GFP) in the jellyfish includes an 11-stranded -barrel encircling the 4-(p-hydroxybenzylidene)imidazolidin-5-one chromophore, which is normally produced autocatalytically1. The crimson fluorescent proteins (DsRed) from coral isomerization from the chromophore in the thrilled state, regarding rotation around imidazolinone exocyclic connection (I-bond)6. Furthermore to I-bond isomerization, the rotation around phenyl (P-) connection may also be extremely efficient (occasionally barrierless and on picosecond timescale) in the isolated chromophore7,8,9,10,11,12,13,14. In mFruits and DsRed, fast reversible bleaching seen in mass tests15,16, fluorescence relationship17,18,19,20, and one molecule spectroscopy20,21 ...
http://stopvivisection.info/tag/ic-87114-novel-inhibtior/
Discosoma sp. 15Discosoma sp. 15
Discosoma sp. 15is commonly referred to as Mushroom. Difficulty in the aquarium: Bardzo łatwy. A aquarium size of at least 50 Liter is recommended.
https://pl.reeflex.net/tiere/1047_Discosoma_sp_15.htm
A new bivalent fluorescent fusion protein for differential Cu(II) and Zn(II) ion detection in aqueous solution -...A new bivalent fluorescent fusion protein for differential Cu(II) and Zn(II) ion detection in aqueous solution -...
Simple and easy to engineer metal-sensing molecules that are capable of differentiating metal ions and producing metal-specific signals are highly desirable. Metal ions affect the thermal stability of proteins by increasing or decreasing their resistance to unfolding. This work illustrates a new strategy for designing bivalent fluorescent fusion proteins capable of differentiating metal ions in solution through their distinct effects on a proteins thermal stability. A new dual purpose metal sensor was developed consisting of biotin protein ligase (BirA) from B. pseudomallei (Bp) fused to green fluorescent protein (GFP). When coupled with differential scanning fluorimetry of GFP-tagged proteins (DSF-GTP) for signal-transduction detection, Bp BirA-GFP yields distinct protein unfolding signatures with Zn(II) and Cu(II) ions in aqueous solutions. The limit of detection of the system is ∼1 μM for both metal species. The system can be used in a variety of high-throughput assay formats including ...
https://researchonline.jcu.edu.au/61890/
Functional Expression of the Aequorea victoria Green Fluorescent Protein in Insect Cells Using the Baculovirus Expression...Functional Expression of the Aequorea victoria Green Fluorescent Protein in Insect Cells Using the Baculovirus Expression...
Author: Reiländer, Helmut et al.; Genre: Journal Article; Published in Print: 1996-02-06; Title: Functional Expression of the Aequorea victoria Green Fluorescent Protein in Insect Cells Using the Baculovirus Expression System
https://pure.mpg.de/pubman/faces/ViewItemOverviewPage.jsp?itemId=item_3262407
Structural investigations on green fluorescent protein variants and th
Structural investigations on green fluorescent protein variants and the adenylyl cyclase associated protein [Elektronische Ressource] / Dorota Ksiazek : Technische Universität München Institut für Organische Chemie und Biochemie Max-Planck-Institut für Biochemie Abteilung Strukturforschung Biologische NMR-Arbeitsgruppe Structural Investigations on Green Fluorescent Protein Variants and the Adenylyl Cyclase-Associated Protein Dorota Ksiazek Vollständiger Abdruck der von der Fakultät für Chemie der Technischen Universität München zur
https://en.youscribe.com/catalogue/documents/knowledge/structural-investigations-on-green-fluorescent-protein-variants-and-1420702
Green fluorescent protein mutant as label in homogeneous assays for biomolecules<...
TY - JOUR. T1 - Green fluorescent protein mutant as label in homogeneous assays for biomolecules. AU - Deo, Sapna K.. AU - Daunert, Sylvia. PY - 2001/2/1. Y1 - 2001/2/1. N2 - The green fluorescent protein (GFP) and its mutants have been extensively used to study various cellular processes and, more recently, as labels in binding assays. We have employed a mutant of GFP, an enhanced GFP (EGFP), in the development of homogeneous assays for biotin and cortisol. To demonstrate the feasibility of using EGFP as a label with different kinds of binders in the development of homogeneous assays, we employed the biotin-avidin and an antigen-antibody as the binding pairs. Biotin and cortisol were chemically conjugated to EGFP. A quenching of fluorescence intensity of EGFP was observed upon binding of avidin to the EGFP-biotin conjugate. The percentage fluorescence quenching observed decreased as the concentration of free biotin in the sample increased due to the fewer binding sites on avidin available for ...
https://miami.pure.elsevier.com/en/publications/green-fluorescent-protein-mutant-as-label-in-homogeneous-assays-f
RCSB PDB - 1W7U: Photoproduct of the Wild-Type Aequorea victoria Green Fluorescent Protein after structural annealing at 170KRCSB PDB - 1W7U: Photoproduct of the Wild-Type Aequorea victoria Green Fluorescent Protein after structural annealing at 170K
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
https://www.rcsb.org/structure/1W7U
Engineering ESPT pathways based on structural analysis of LSSmKate red fluorescent proteins with large stokes shift<...Engineering ESPT pathways based on structural analysis of LSSmKate red fluorescent proteins with large stokes shift<...
TY - JOUR. T1 - Engineering ESPT pathways based on structural analysis of LSSmKate red fluorescent proteins with large stokes shift. AU - Piatkevich, Kiryl D.. AU - Malashkevich, Vladimir N.. AU - Almo, Steven C.. AU - Verkhusha, Vladislav. PY - 2010/8/11. Y1 - 2010/8/11. N2 - LSSmKate1 and LSSmKate2 are monomeric red fluorescent proteins (RFPs) with large Stokes shifts (LSSs), which allows for efficient separation of absorbance and emission maxima, as well as for excitation with conventional two-photon laser sources. These LSSmKates differ by a single amino acid substitution at position 160 and exhibit absorbance maxima around 460 nm, corresponding to a neutral DsRed-like chromophore. However, excitation at 460 nm leads to fluorescence emission above 600 nm. Structures of LSSmKate1 and LSSmKate2, determined at resolutions of 2.0 and 1.5, respectively, revealed that the predominant DsRed-chromophore configurations are cis for LSSmKate1 but trans for LSSmKate2. Crystallographic and mutagenesis ...
https://einstein.pure.elsevier.com/en/publications/engineering-espt-pathways-based-on-structural-analysis-of-lssmkat-2
FMDV replicons encoding green fluorescent protein are replication competent<...
TY - JOUR. T1 - FMDV replicons encoding green fluorescent protein are replication competent. AU - Tulloch, Fiona. AU - Pathania, Uday. AU - Luke, Garry A.. AU - Nicholson, John. AU - Stonehouse, Nicola J.. AU - Rowlands, David J.. AU - Jackson, Terry. AU - Tuthill, Toby. AU - Haas, Juergen. AU - Lamond, Angus I.. AU - Ryan, Martin D.. PY - 2014/12/1. Y1 - 2014/12/1. N2 - The study of replication of viruses that require high bio-secure facilities can be accomplished with less stringent containment using non-infectious replicon systems. The FMDV replicon system (pT7rep) reported by Mclnerney et al. (2000) was modified by the replacement of sequences encoding chloramphenicol acetyl-transferase (CAT) with those encoding a functional (L pro) linked to a bi-functional fluorescent/antibiotic resistance fusion protein (green fluorescent protein/puromycin resistance, [GFP-PAC]). Cells were transfected with replicon-derived transcript RNA and GFP fluorescence quantified. Replication of transcript RNAs ...
https://discovery.dundee.ac.uk/en/publications/fmdv-replicons-encoding-green-fluorescent-protein-are-replication
VE-821 enzyme inhibitor - biobender.comVE-821 enzyme inhibitor - biobender.com
Supplementary Materialsoncotarget-09-6518-s001. This demonstrates the significant potential of alveolar type II cells in orchestrating the process of metastasis, rendering it as one of the target cell types of the lung of therapeutic importance in human NSCLC. expression is usually replaced with reddish fluorescent protein (DsRed*MST) expression in tissues expressing Cre recombinase. We utilized transgenic mice in which the human SPC (Sftpc) gene promoter is used expressing the invert tetracycline transactivator (rtTA) hence placing the appearance of Cre-recombinase (CRE) beneath the conditional control of doxycycline. Appearance of Cre was utilized to completely label cells with Crimson fluorescent proteins (DsRed) in alveolar type II cells. Distinctive lines of transgenic mice that exhibit rtTA beneath the control of the individual surfactant-associated proteins C (Sftpc/SPC) gene promoter had been bred VE-821 enzyme inhibitor to TetO-Cre mice and reporter mice (LacZ/DsRed) creating triple ...
http://biobender.com/tag/ve-821-enzyme-inhibitor/
Citrine * Golden Age of Light
Citrine, one of the five major types of quartz, is the perfect stone for courage, confidence, self-worth and self-empowerment. Why? Because it cannot hold negative energy. Citrine aligns with the energies of the solar plexus, the subtle bodys home of the will. It is extremely helpful clearing overflowing emotions from the heart or within space innfused with negative energies. By aligning your will with Divine Will, or ones Higher Self, a natural flow of spiritual input and output can be achieved through the human vehicle. Citrine can often be found with rainbow inclusions, the Mineral Kingdoms way of reminding you that there is always hope and something good to be strived for. Citrine is also known as the Merchants Stone as it is a magnet for abundance. Its positive energies, brightness and allure are beneficial to you whether carried as a talisman in your pocket, worn as jewelry or placed in your home or working environment.. Lions Gate, August 8, 2018.. ...
https://goldenageoflight.com/citrine/
1jc1 - Proteopedia, life in 3D1jc1 - Proteopedia, life in 3D
Current methods for determining ambient redox potential in cells are labor-intensive and generally require destruction of tissue. This precludes single cell or real time studies of changes in redox poise that result from metabolic processes or environmental influences. By substitution of surface-exposed residues on the Aequorea victoria green fluorescent protein (GFP) with cysteines in appropriate positions to form disulfide bonds, reduction-oxidation-sensitive GFPs (roGFPs) have been created. roGFPs have two fluorescence excitation maxima at about 400 and 490 nm and display rapid and reversible ratiometric changes in fluorescence in response to changes in ambient redox potential in vitro and in vivo. Crystal structure analyses of reduced and oxidized crystals of roGFP2 at 2.0- and 1.9-A resolution, respectively, reveal in the oxidized state a highly strained disulfide and localized main chain structural changes that presumably account for the state-dependent spectral changes. roGFP1 has been ...
http://proteopedia.org/wiki/index.php/1jc1
Defining the role of arginine 96 in green fluorescent protein fluorophore biosynthesisDefining the role of arginine 96 in green fluorescent protein fluorophore biosynthesis
Aequoria victoria green fluorescent protein (GFP) is a revolutionary molecular biology tool because of its spontaneous peptide backbone cyclization and chromophore formation from residues Ser65, Tyr66, and Gly67. Here we use structure-based design, comprehensive targeted mutagenesis, and high-resolution crystallography to probe the significant functional role of conserved Arg96 (R96) in chromophore maturation. The R96M GFP variant, in which the R96M side chain is similar in volume but lacks the R96 positive charge, exhibits dramatically slower chromophore maturation kinetics (from hours to months). Comparison of the precyclized conformation of the chromophore-forming residues with the mature R96M chromophore reveals a similar Y66 conformer, contrary to the large Y66 conformational change previously defined in the slowly maturing R96A variant [Barondeau, D. P., Putnam, C. D., Kassmann, C. J., Tainer, J. A., and Getzoff, E. D. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 12111-12116]. Comprehensive ...
https://vivo.scripps.edu/display/endnote8904
Difference between revisions of Group 9 Project - Fluorescent Proteins - CellBiologyDifference between revisions of Group 9 Project - Fluorescent Proteins - CellBiology
References for The Development of New FPs 15. Shaner , N C, Patterson, G H, & Davidson, M W. (2007). Advances in fluorescent protein technology . Journal of Cell Science, 120. PMID: 18057027 16. Shaner, N C, Steinbach, P A & Tsien, R Y. (2005). A guide to choosing fluorescent proteins. Nature Methods, 2, PMID: 16299475 17. Ai, H, Shaner, N C, Cheng, Z, Tsien, R Y & Campbell, R E. (2007). Exploration of new chromophore structures leads to the identification of improved blue fluorescent proteins. Biochemistry, 46, PMID: 17444659 18. Kremers, G J, Goedhart, J, van den Heuvel, D J, Gerritsen, H C. & Gadella, T W J. (2007). Improved green and blue fluorescent proteins for expression in bacteria and mammalian cells. Biochemistry, 46, PMID: 17323929 19. Cubitt, A B., Heim, R, Adams, S R, Boyd, A E, Gross, L A & Tsien, R Y. (1995). Understanding, improving and using green fluorescent proteins. Trends Biochemical Science, 20, PMID: 8578587 20. Rizzo, M A, Springer, G H, Granada, B & Piston, D W. (2004). ...
https://cellbiology.med.unsw.edu.au/cellbiology/index.php?title=Group_9_Project_-_Fluorescent_Proteins&diff=next&oldid=22481
Group 9 Project - Fluorescent Proteins - CellBiologyGroup 9 Project - Fluorescent Proteins - CellBiology
References for The Development of New FPs 15. Shaner , N C, Patterson, G H, & Davidson, M W. (2007). Advances in fluorescent protein technology . Journal of Cell Science, 120. PMID: 18057027 16. Shaner, N C, Steinbach, P A & Tsien, R Y. (2005). A guide to choosing fluorescent proteins. Nature Methods, 2, PMID: 16299475 17. Ai, H, Shaner, N C, Cheng, Z, Tsien, R Y & Campbell, R E. (2007). Exploration of new chromophore structures leads to the identification of improved blue fluorescent proteins. Biochemistry, 46, PMID: 17444659 18. Kremers, G J, Goedhart, J, van den Heuvel, D J, Gerritsen, H C. & Gadella, T W J. (2007). Improved green and blue fluorescent proteins for expression in bacteria and mammalian cells. Biochemistry, 46, PMID: 17323929 19. Cubitt, A B., Heim, R, Adams, S R, Boyd, A E, Gross, L A & Tsien, R Y. (1995). Understanding, improving and using green fluorescent proteins. Trends Biochemical Science, 20, PMID: 8578587 20. Rizzo, M A, Springer, G H, Granada, B & Piston, D W. (2004). ...
https://cellbiology.med.unsw.edu.au/cellbiology/index.php?title=Group_9_Project_-_Fluorescent_Proteins&oldid=22676&printable=yes
RCSB PDB - 2DUF: crystal structure of a green fluorescent protein variant S65T/H148D at pH 5.6RCSB PDB - 2DUF: crystal structure of a green fluorescent protein variant S65T/H148D at pH 5.6
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
https://www.rcsb.org/structure/2DUF
Time-lapse FRET microscopy using fluorescence anisotropy. - Department of Oncology
We present recent data on dynamic imaging of Rac1 activity in live T-cells. Förster resonance energy transfer between enhanced green and monomeric red fluorescent protein pairs which form part of a biosensor molecule provides a metric of this activity. Microscopy is performed using a multi-functional high-content screening instrument using fluorescence anisotropy to provide a means of monitoring protein-protein activity with high temporal resolution. Specifically, the response of T-cells upon interaction of a cell surface receptor with an antibody coated multi-well chamber was measured. We observed dynamic changes in the activity of the biosensor molecules with a time resolution that is difficult to achieve with traditional methodologies for observing Förster resonance energy transfer (fluorescence lifetime imaging using single photon counting or frequency domain techniques) and without spectral corrections that are normally required for intensity based methodologies.
https://www.oncology.ox.ac.uk/publications/72872/
Natural Congo Pale Citrine Crystal - 71mm, 46g - Deep Earth TreasuresNatural Congo Pale Citrine Crystal - 71mm, 46g - Deep Earth Treasures
Locality - Lwena, Democratic Republic of Congo Size - 71 x 29 x 23mm Weight - 46g This citrine crystal displays cascading side crystalline formation climbing up the main termination point and has nice, pale golden colour at its apex with a subtle rainbow reflective veil within (as photographed). All of our citrine crys
https://www.deepearthtreasures.com/products/natural-congo-pale-citrine-crystal-71mm-46g
18k Gold Lemon Citrine Button Earrings | Citrine | Earrings | Jewelry | ScullyandScully.com18k Gold Lemon Citrine Button Earrings | Citrine | Earrings | Jewelry | ScullyandScully.com
Stunning 16Mm Lemon Citrine Gemstones Are Bezel Set In 18K Gold With Beaded Borders These Earrings Provide A Rich Accent Of Color For Day Or Evening
https://www.scullyandscully.com/jewelry/earrings/citrine/18k-gold-lemon-citrine-button-earrings.axd
GFP - Normal Human Vascular Smooth Muscle Cell Culture ResearchGFP - Normal Human Vascular Smooth Muscle Cell Culture Research
Intensive searches for novel green fluorescent protein (GFP)-like fluorescent proteins have identified more than 150 distinct genes that, together with its mutants, cover the excitation range from 380 to 600 nanometers (nm) and the emission range from 440 to 650 nm (see table below). Despite spectral diversity, a family of GFP-like proteins possesses common significant structural, biochemical and photophysical features. Many of these spectroscopically active proteins are developed to commercially available genetically-encoded fluorescent probes. In comparison to other natural pigments and fluorophores, GFP-like proteins stand out because they form internal chromophores without requiring accessory cofactors, external enzymatic catalysis or substrates other than molecular oxygen. It gives GFP-like proteins many advantages including that chromophore formation is possible in live organisms, tissues or cells while maintaining their integrity as well as molecular, organelle and tissue targeting and ...
https://promuscle.org/gfp/
Applications of the green fluorescent protein in cell biology and biotechnology - CSHL Scientific Digital RepositoryApplications of the green fluorescent protein in cell biology and biotechnology - CSHL Scientific Digital Repository
The recent emergence of an autofluorescent protein, the green fluorescent protein (GFP), has opened the door for the convenient use of intact living cells and organisms as experimental systems in fields ranging from cell biology to biomedicine. We present an overview of some of the major applications of GFP, namely its use in protein tagging and in monitoring gene expression as well as its potential in a variety of biological screens.. ...
http://repository.cshl.edu/id/eprint/26337/
Difference between revisions of CH391L/S13/Metagenomics & Bioprospecting - OpenWetWareDifference between revisions of CH391L/S13/Metagenomics & Bioprospecting - OpenWetWare
Fluorescent proteins are likely one of the most famous research tools derived from bioprospecting. Examples include dsRed as well as GFP and its many derivatives, which have been utilized throughout biological research. Interestingly, these fluorescent proteins are finding new purpose in medicine as visual guides during surgery. Before tumorectomy, a mouse with internal tumors is injected with a recombinant form of GFP, which is targeted to and accumulates on the cells of blood vessels. During surgical removal of the tumor, the introduced GFP provides a surgeon with a strong visual queue of nearby blood vessels greatly reducing the risk of blood vessel lacerations. DNA and RNA polymerases are the workhorses of modern biotechnology. Almost every aspect of modern biological research depends upon nucleic acid polymerases in one way or another. Recombinant cloning techniques, Sanger sequencing, and qPCR cover a few of the most common uses. These examples also highlight the shared importance of ...
https://openwetware.org/wiki/?title=CH391L/S13/Metagenomics_%252526_Bioprospecting:_Part_Deux&curid=130731&diff=678969&oldid=678968
DNA sequence-enabled reassembly of the green fluorescent protein<...
TY - JOUR. T1 - DNA sequence-enabled reassembly of the green fluorescent protein. AU - Stains, Cliff I.. AU - Porter, Jason R.. AU - Ooi, Aik T.. AU - Segal, David J.. AU - Ghosh, Indraneel. PY - 2005/8/10. Y1 - 2005/8/10. N2 - We describe a general methodology for the direct detection of DNA by the design of a split-protein system that reassembles to form an active complex only in the presence of a targeted DNA sequence. This approach, called SEquence Enabled Reassembly (SEER) of proteins, combines the ability to rationally dissect proteins to construct oligomerization-dependent protein reassembly systems and the availability of DNA binding Cys2-His2 zinc-finger motifs for the recognition of specific DNA sequences. We demonstrate the feasibility of the SEER approach utilizing the split green fluorescent protein appended to appropriate zinc fingers, such that chromophore formation is only catalyzed in the presence of DNA sequences that incorporate binding sites for both zinc fingers.. AB - We ...
https://experts.nebraska.edu/en/publications/dna-sequence-enabled-reassembly-of-the-green-fluorescent-protein
Structural characterization of the photoswitchable fluorescent protein Dronpa-C62S<...
TY - JOUR. T1 - Structural characterization of the photoswitchable fluorescent protein Dronpa-C62S. AU - Nam, Ki Hyun. AU - Kwon, Oh Yeun. AU - Sugiyama, Kanako. AU - Lee, Won Ho. AU - Kim, Young Kwan. AU - Song, Hyun Kyu. AU - Kim, Eunice Eunkyung. AU - Park, Sam Yong. AU - Jeon, Hyesung. AU - Hwang, Kwang Yeon. N1 - Funding Information: We thank Dr. H.S. Lee and his staff for assistance during data collection at beamline 4A of Pohang Light Source, Korea. H.J. is supported by Grant M10420010001-04N2001-00110 from MOST, Korea and by the Molecular Imaging Program at the Korea Institute of Science and Technology. K.Y.H. is supported by the Functional Proteomics Center, 21C Frontier Program of the Korea Ministry of Science and Technology. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.. PY - 2007/3/23. Y1 - 2007/3/23. N2 - The photoswitching behavior of green fluorescent proteins (GFPs) or GFP-like proteins is increasingly recognized as a new technique for optical marking. Recently, ...
https://koreauniv.pure.elsevier.com/en/publications/structural-characterization-of-the-photoswitchable-fluorescent-pr
Addgene: Bright cyan fluorescent protein variants identified by fluorescence lifetime screening.Addgene: Bright cyan fluorescent protein variants identified by fluorescence lifetime screening.
Please note: Your browser does not support the features used on Addgenes website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more ...
https://www.addgene.org/browse/article/9130/
The green fluorescent protein (GFP) is the most commonly used reporter | kinase inhibitor tool compounds for pharmacological...The green fluorescent protein (GFP) is the most commonly used reporter | kinase inhibitor tool compounds for pharmacological...
The green fluorescent protein (GFP) is the most commonly used reporter protein for monitoring gene expression and protein localization in a variety of living and fixed cells, including not only prokaryotes, but eukaryotes also, e. EGFP inhibited both cell nest and growth development, and activated cell loss of life in Ku80-lacking hamster cells, i.y., xrs-6 cells. In addition, Ku80 attenuated EGFP-induced cytotoxicity in the xrs-6 cells. No EGFP-induced cytotoxicity was noticed in the NHEJ primary proteins XRCC4-lacking hamster cells, i.y., XR-1 cells. Furthermore, Substantially enhanced X-ray-induced cytotoxicity in the xrs-6 cells EGFP. These outcomes recommend that Ku80 has a essential function in the story NHEJ-independent protection system against EGFP-induced cytotoxicity. Extreme care should end up being used in taking into consideration of the potential impact by the tension response system, specifically, the Ku80-reliant reduction system of EGFP-induced cytotoxicity, getting turned on, ...
http://cdkkinase.com/the-green-fluorescent-protein-gfp-is-the-most-commonly-used-reporter/
Journal Club for December 2017: Mechanics of living mammalian cytoplasm | iMechanica
Mechanics of living mammalian cytoplasm 1.Overview The cytoplasm of living mammalian cells is a crowded, yet dynamic environment(1). It provides the key physical environment to the cellular factory and all intracellular physiological processes from transcription, translation, to protein binding and folding. Therefore, understanding the fundamental physical nature of the cytoplasm is critical to understanding the basic physiology of cells. Moreover, there are continuous intracellular movements that are vital for cell function, such as transport of vesicles and other organelles. While biological motors and other enzymatic processes provide key driving forces for these activities, the mechanical behavior of the cytoplasm are crucial for determining the mechanical resistance that cellular compartments experience. Both the active driving force and appropriate mechanical environment are critical for shaping the living cellular machinery. However, while the force that molecular motors generate both ...
https://imechanica.org/node/21924
Molecular Expressions Microscopy Primer: Specialized Microscopy Techniques - Fluorescence Digital Image Gallery - Human Bone...Molecular Expressions Microscopy Primer: Specialized Microscopy Techniques - Fluorescence Digital Image Gallery - Human Bone...
The culture of human osteosarcoma cells featured in this section was transfected with a pDsRed-Mitochondria plasmid subcellular localization vector, thus localizing a red fluorescent protein tag to the intracellular mitochondrial network.
https://micro.magnet.fsu.edu/primer/techniques/fluorescence/gallery/cells/u2/u2cellslarge5.html
Golden Citrine 10k Yellow Gold Stud Earrings .20ctw - CWP019L | JTV.comGolden Citrine 10k Yellow Gold Stud Earrings .20ctw - CWP019L | JTV.com
Add some sparkle to your look with JTVs gorgeous Golden Citrine 10k Yellow Gold Stud Earrings .20ctw! Shop JTV jewelry today for an incredible deal!
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Bypass Oval Pave Phala Aquamarine Ring with Ruby and Citrine in Palladium | AzeeraBypass Oval Pave Phala Aquamarine Ring with Ruby and Citrine in Palladium | Azeera
This modern ring is timeless and elegant. Your oval cut gem sits in a 4 prong bypass setting. In between walls, a pave of smaller round brilliant gems runs along the upper half of the band. This Aquamarine ring is casted in Palladium, set with Natural Aquamarine, Ruby and Citrine. Your Modern gemstone ring also comes with Free Shipping, Free Returns, and a Lifetime Warranty.
https://www.azeera.com/bypass-oval-pave-phala-ring-az1004-7x5-aq-rb-ct-pd
SOLAR PLEXUS Citrine
citrine, protection, negative, energy, protect, jewelry, healing, gemstone, heart, love, heal, mind, joy, express, self-esteem, clarity, grounding, cash, money, rich, job, creation, active, accomplish, work, ideas, hope, solar plexus, cash, kidney,
http://shop.oholivia.com/Citrine-SOLAR-PLEXUS-CHAKRA-Pendant-OO17-04.htm
Photoswitchable protein molecule - Stock Image C035/6331 - Science Photo LibraryPhotoswitchable protein molecule - Stock Image C035/6331 - Science Photo Library
Photoswitchable protein IrisFP molecule. Computer model showing the structure of the reversibly photoswitchable green to red fluorescent protein IrisFP. - Stock Image C035/6331
http://www.sciencephoto.com/media/847904/view
Hempz Citrine Crystal & Quartz Herbal Body Buff | Ulta BeautyHempz Citrine Crystal & Quartz Herbal Body Buff | Ulta Beauty
Uplift your skin and mood and with Hempz Citrine Crystal & Quartz Herbal Body Buff! Sparkling, crushed exfoliators gently smooth and rejuvenate skin, energized with H20, Vitamins, nutrients, anti-oxidants and quartz for hydrated, illuminating, healthy looking skin.
https://www.ulta.com/citrine-crystal-quartz-herbal-body-buff?productId=pimprod2004186
Vertical pedigree: CH Citrines EZr SedDanDun
CH Citrines EZr SedDanDun. A vertical pedigree lists the siblings of each dog in the pedigree. Siblings are found by searching for dogs in the database with the same parents. They may be from the same litter or from another breeding of the same parents. In most cases, this pedigree will be incomplete, as not all dogs have all their siblings entered in the database.. Vertical pedigree: ...
http://www.k9data.com/verticalpedigree.asp?ID=29114
Citrine Quartz<...Citrine Quartz<...
Citrine is one of a very few stones that does not hold on to or accumulate negative energy, but dissipates and transmutes it., working out problems on both the physical and subtle levels. It hence, never needs clearing or cleansing...
http://www.exquisitecrystals.com/quartz/citrine-quartz
Nest - Citrine Eau de Parfum | Candle DeliriumNest - Citrine Eau de Parfum | Candle Delirium
Nest Citrine Eau de Parfum isthe dewy softness of lotus flower and freesia, set against sparkling citrus notes and hints of precious woods.
https://www.candledelirium.com/nest-citrine-eau-de-parfum/
Multi-parameter analysis of the kinetics of NF-κB signalling and transcription in single living cells | Journal of Cell ScienceMulti-parameter analysis of the kinetics of NF-κB signalling and transcription in single living cells | Journal of Cell Science
We report for the first time the rela time non-invasive kinetic analysis of three steps in the NF-κB signalling pathway; IκBα degradation, p65 translocation and NF-κB-dependent transcription. We have used these tools to investigate the link between the kinetics of the NF-κB pathway, the levels of NF-κB and IκB proteins in cell compartments, and the resulting timing of transcription.. We showed that both the p65-EGFP and p65-dsRed fluorescent fusion proteins gave rise to the nuclear translocation in response to TNFα treatment, which is characteristic of the functional endogenous protein. Ding et al. previously reported an endogenous p65 nuclear translocation half time of 7-8 minutes in HeLa cells following TNFα stimulation ( Ding et al., 1998). In comparison, we obtained a longer half time of 19±2.9 minutes for nuclear translocation of p65-EGFP in singly transfected cells ( Fig. 1A) in agreement with other studies using a p65-EGFP fluorescent fusion protein and stimulation with IL-1β ( ...
http://jcs.biologists.org/content/115/6/1137
Enhanced Yellow Fluorescent Protein (EYFP) Golgi Localization | Nikons MicroscopyUEnhanced Yellow Fluorescent Protein (EYFP) Golgi Localization | Nikons MicroscopyU
The collection of specimens illustrated in this section demonstrates the effectiveness of the Nikon YFP HYQ filter combination with a series of cell lines containing a chimeric EYFP plasmid vector that expresses a fluorescent fusion protein targeted at the intracellular Golgi apparatus.
https://www.microscopyu.com/techniques/fluorescence/nikon-fluorescence-filter-sets/yellow-fluorescent-protein-excitation-yfp-hyq/enhanced-yellow-fluorescent-protein-eyfp-golgi-localization
Enhanced Yellow Fluorescent Protein (EYFP) Endosome Localization | MicroscopyUEnhanced Yellow Fluorescent Protein (EYFP) Endosome Localization | MicroscopyU
The collection of specimens illustrated in this section demonstrates the effectiveness of the Nikon YFP HYQ filter combination with a series of cell lines containing a chimeric EYFP plasmid vector that expresses a fluorescent fusion protein targeted at the cellular endosomal network.
https://www.microscopyu.com/techniques/fluorescence/nikon-fluorescence-filter-sets/yellow-fluorescent-protein-excitation-yfp-hyq/enhanced-yellow-fluorescent-protein-eyfp-endosome-localization
Evrogen pKatushka2S-N (CAT.# FP762)Evrogen pKatushka2S-N (CAT.# FP762)
pKatushka2S-N is a mammalian expression vector encoding far-red fluorescent protein Katushka2S (see reporter description). The vector allows generation of fusions to the Katushka2S N-terminus and expression of Katushka2S fusions or Katushka2S alone in eukaryotic (mammalian) cells. Katushka2S codon usage is optimized for high expression in mammalian cells (humanized) [Haas et al., 1996]. To increase mRNA translation efficiency, Kozak consensus translation initiation site is generated upstream of the Katushka2S coding sequence [Kozak, 1987]. Multiple cloning site (MCS) is located between PCMV IE and Katushka2S coding sequence. The vector backbone contains immediate early promoter of cytomegalovirus (PCMV IE) for protein expression, SV40 origin for replication in mammalian cells expressing SV40 T-antigen, pUC origin of replication for propagation in E. coli, and f1 origin for single-stranded DNA production. SV40 polyadenylation signals (SV40 poly A) direct proper processing of the 3-end of the ...
https://evrogen.com/products/vectors/pKatushka2S-N/pKatushka2S-N.shtml
Molecular Expressions Microscopy Primer: Specialized Microscopy Techniques - Spectral Imaging and Linear UnmixingMolecular Expressions Microscopy Primer: Specialized Microscopy Techniques - Spectral Imaging and Linear Unmixing
Spectral imaging and linear unmixing has become an important tool in confocal and widefield fluorescence microscopy to discriminate between fluorophores having overlapping spectral characteristics.
https://micro.magnet.fsu.edu/primer/techniques/fluorescence/spectralimaging/index.html
Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications | ProtocolGenetic Barcoding with Fluorescent Proteins for Multiplexed Applications | Protocol
Since the discovery of the green fluorescent protein gene, fluorescent proteins have impacted molecular cell biology. This protocol...
https://www.jove.com/video/52452/genetic-barcoding-with-fluorescent-proteins-for-multiplexed
ATP sensing in living plant cells reveals tissue gradients and stress dynamics of energy physiology | Department of Plant...
Congratulations to Dr Mark Fricker for his work on the paper ATP sensing in living plant cells reveals tissue gradients and stress dynamics of energy physiology that was published open access on the eLIFE website.. Read the paper here: https://doi.org/10.7554/eLife.26770. Press release: https://www.uni-bonn.de/news/178-2017. ...
http://www.plants.ox.ac.uk/news/atp-sensing-living-plant-cells-reveals-tissue-gradients-and-stress-dynamics-energy-physiology-0
Prof. Dr. Conrad W. Mullineaux - Freiburg Institute for Advanced Studies - FRIAS
In search of localised membrane protein assembly centres in bacteria. PspA (Phage-Shock Protein A) is a widespread bacterial protein known to be important for preserving membrane integrity under environmental stress conditions. The related Vipp1 protein is found in cyanobacteria (and plant chloroplasts) and seems to play a crucial role in the biogenesis of photosynthetic membranes. In both case, the mechanism of action of the protein is enigmatic. We have visualised both proteins in vivo using fluorescence microscopy and fluorescent protein tags, and found that they form clusters near to the membrane under the conditions when they are likely to be physiologically active. Combined with biochemical identification of interaction partners under these conditions, this suggests a novel hypothesis: that both these proteins may help to organise assembly centres for the rapid and localised production of membrane and secreted proteins. A key prediction of this hypothesis is that specific mRNA molecules ...
http://www.frias.uni-freiburg.de/en/people/fellows/current-fellows/mullineaux
Lanthanide probesLanthanide probes
Turro, C; Fu, PK; Bradley, PM (2003). "Lanthanide ions as luminescent probes of proteins and nucleic acids". Metal Ions in ... polyaminocarboxylates as luminescent sensors in time-resolved luminescent (TRL) immunoassays. Optimization of analytical ... The luminescent probe may for instance serve to localize the MRI contrast agent. This has helped to visualize the delivery of ... LRET was also used to study the interaction of the proteins dystrophin and actin in muscle cells. Dystrophin is present in the ...
https://en.wikipedia.org/wiki/Lanthanide_probes
Stern-Volmer relationshipStern-Volmer relationship
Permyakov, Eugene A. [Luminescent Spectroscopy of Proteins], CRC Press, 1993. Fluorescence lifetimes and dynamic quenching ( ...
https://en.wikipedia.org/wiki/Stern–Volmer_relationship
Outline of photographyOutline of photography
Bioluminescence imaging - a technique for studying laboratory animals using luminescent protein. Calcium imaging - determining ...
https://en.wikipedia.org/wiki/Outline_of_photography
Renilla-luciferin 2-monooxygenaseRenilla-luciferin 2-monooxygenase
Recently, chimeras of RLuc have been developed and demonstrated to be the brightest luminescent proteins to date, and have ... "Luminescent proteins for high-speed single-cell and whole-body imaging". Nature Communications. 3: 1262. Bibcode:2012NatCo... ... Protein Engineering. 5 (3): 197-211. doi:10.1093/protein/5.3.197. hdl:11370/2d4c057d-1a67-437d-ad10-701f7a60f1e6. PMID 1409539 ... Protein Engineering, Design & Selection. 19 (9): 391-400. doi:10.1093/protein/gzl023. PMID 16857694. Anderson JM, Charbonneau H ...
https://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase
Marc ZimmerMarc Zimmer
"Luminescent Eel Muscles Fluorescent Protein Revolution into Clinic." Huffington Post, June 18, 2013. "Mending Broken Hearts: ... "From CRISPR to glowing proteins to optogenetics - scientists' most powerful technologies have been borrowed from nature." The ... "AI makes huge progress predicting how proteins fold - one of biology's greatest challenges - promising rapid drug development ...
https://en.wikipedia.org/wiki/Marc_Zimmer
NanomedicineNanomedicine
A way around this problem is with luminescent tags. These tags are quantum dots attached to proteins that penetrate cell ... These devices are built with nanowires to detect cancer proteins; each nanowire detector is primed to be sensitive to a ... Binding agents such as proteins, antibiotics, or synthetic ligands are covalently linked to the particle surface. These binding ... able to detect proteins and other biomarkers left behind by cancer cells, could enable the detection and diagnosis of cancer in ...
https://en.wikipedia.org/wiki/Nanomedicine
COVID-19 testingCOVID-19 testing
The result is luminescent. A chemiluminescent microparticle immunoassay uses magnetic, protein-coated microparticles. ... A plate is coated with a viral protein, such as a SARS-CoV-2 spike protein. Samples are incubated with the protein, allowing ... Antigen tests look for antigen proteins from the viral surface. In the case of a coronavirus, these are usually proteins from ... "A SARS-CoV-2 surrogate virus neutralization test based on antibody-mediated blockage of ACE2-spike protein-protein interaction ...
https://en.wikipedia.org/wiki/COVID-19_testing
Fluorescence in the life sciencesFluorescence in the life sciences
Lamture, JB; Wensel, TG (1995). "Intensely luminescent immunoreactive conjugates of proteins and dipicolinate-based polymeric ... phycoerythrin or green fluorescent protein). Alternatively, specific or general proteins, nucleic acids, lipids or small ... Other proteins are fluorescent but require a fluorophore cofactor, and hence can only be used in vitro; these are often found ... Several fluorescent protein exist in nature[citation needed], but the most important one as a research tool is Green ...
https://en.wikipedia.org/wiki/Fluorescence_in_the_life_sciences
AequorinAequorin
"Cloning and sequence analysis of cDNA for the luminescent protein aequorin". Proc. Natl. Acad. Sci. U.S.A. 82 (10): 3154-58. ... "Prediction of EF-hand calcium-binding proteins and analysis of bacterial EF-hand proteins". Proteins. 65 (3): 643-655. doi: ... Notably, the protein contains three EF hand motifs that function as binding sites for Ca2+ ions. The protein is a member of the ... In the animal, the protein occurs together with the green fluorescent protein to produce green light by resonant energy ...
https://en.wikipedia.org/wiki/Aequorin
Thalappil PradeepThalappil Pradeep
A number of atomically precise luminescent clusters have been made in proteins and their growth involves inter-protein metal ... "Understanding the Evolution of Luminescent Gold Quantum Clusters in Protein Templates". ACS Nano. 5 (11): 8816-8827. doi: ... Combining luminescent atomically precise clusters with mesoflowers and nanofibres, he developed sensors at sub-zeptomole levels ... 4. Noble metal clusters in protein templates, T. Pradeep, A. Baksi and P. L. Xavier in Functional nanometer-sized clusters of ...
https://en.wikipedia.org/wiki/Thalappil_Pradeep
Reporter geneReporter gene
... used reporter genes that induce visually identifiable characteristics usually involve fluorescent and luminescent proteins. ... The mRNA is then translated into protein. It is important that both proteins be able to properly fold into their active ... Nordgren, I. K.; Tavassoli, A (2014). "A bidirectional fluorescent two-hybrid system for monitoring protein-protein ... A more complex use of reporter genes on a large scale is in two-hybrid screening, which aims to identify proteins that natively ...
https://en.wikipedia.org/wiki/Reporter_gene
Lomonosov Gold MedalLomonosov Gold Medal
Martin Chalfie (professor, United States): for developing new methods for bioluminescent analysis using GFP luminescent protein ...
https://en.wikipedia.org/wiki/Lomonosov_Gold_Medal
LactoferrinLactoferrin
"Luminescent quantum clusters of gold in transferrin family protein, lactoferrin exhibiting FRET" (PDF). Nanoscale. 2 (12): 2769 ... The fraction of protein extracted from milk, contains 3.3% RNA, but, the protein preferably binds to double-stranded DNA rather ... Occurrence of iron-containing red protein in bovine milk was reported as early as in 1939; however, the protein could not be ... optical absorption spectra and presence of two iron atoms per protein molecule. The protein was extracted from milk, contained ...
https://en.wikipedia.org/wiki/Lactoferrin
Amyloid plaquesAmyloid plaques
Amyloid beta (Aβ) is a small protein, most often 40 or 42 amino acids in length, that is released from a longer parent protein ... Klingstedt T; Nilsson KPR (2012). "Luminescent conjugated poly- and oligo-thiophenes: optical ligands for spectral assignment ... The misfolded proteins stick to one another, eventually stacking together to form oligomers that merge to make the amyloid ... The normal function of Aβ is not certain, but plaques arise when the protein misfolds and begins to accumulate in the brain by ...
https://en.wikipedia.org/wiki/Amyloid_plaques
PhotoproteinPhotoprotein
... s are a type of enzyme, made of protein, from bioluminescent organisms. They add to the function of the luciferins ... The term photoprotein was first used to describe the unusual chemistry of the luminescent system of Chaetopterus (a marine ... Polychaete worm). This was meant to distinguish them from other light-producing proteins because these do not exhibit the usual ...
https://en.wikipedia.org/wiki/Photoprotein
CalmodulinCalmodulin
The AtBAG6 protein is a CaM-binding protein that binds to CaM only in the absence of Ca2+ and not in the presence of it. AtBAG6 ... Kilhoffer MC, Demaille JG, Gerard D (July 1980). "Terbium as luminescent probe of calmodulin calcium-binding sites; domains I ... "Protein-peptide interaction studies demonstrate the versatility of calmodulin target protein binding". Protein and Peptide ... CaM binding proteins are also known to regulate reproductive development in plants. For instance, the CaM-binding protein ...
https://en.wikipedia.org/wiki/Calmodulin
CoelenterazineCoelenterazine
All these proteins catalyze the oxidation of this substance, a reaction catalogued EC 1.13.12.5. Coelenterazine was ... Both groups independently discovered that the same compound was used in both luminescent systems. The molecule was named after ... Myctophidae echinoderms such as Amphiura filiformis The compound has also been isolated from organisms that are not luminescent ... simultaneously isolated and characterized by two groups studying the luminescent organisms sea pansy (Renilla reniformis) and ...
https://en.wikipedia.org/wiki/Coelenterazine
Gerd Ulrich NienhausGerd Ulrich Nienhaus
There, he continued his research on heme proteins, studying ligand migration within these proteins and its effects on the ... He has also been engaged in the development and characterization of nanoscale luminescent markers for bioimaging (fluorescent ... his laboratory carried on with studies of ligand binding and protein dynamics, mainly on heme proteins. Since 1997, he has been ... "Structural basis for photo-induced protein cleavage and green-to-red conversion of fluorescent protein EosFP". Proceedings of ...
https://en.wikipedia.org/wiki/Gerd_Ulrich_Nienhaus
Antibiotic sensitivity testingAntibiotic sensitivity testing
These involve labelled proteins targeted at biomarkers, nucleic acid sequences present within cells that are found when the ... The isolate is then exposed to fluorescent dye, which will be luminescent when viewed. Improvements to existing platforms are ...
https://en.wikipedia.org/wiki/Antibiotic_sensitivity_testing
Anthony Campbell (British biochemist)Anthony Campbell (British biochemist)
Extraction, partial purification and properties of obelin, the calcium- activated luminescent protein from the hydroid Obelia ... He is an expert on bioluminescence, having developed the use of genetically engineered bioluminescent proteins to measure ... pioneering the application of Ca2+-activated bioluminescent proteins to measure free Ca2+ in live animal, plant, bacterial, and ...
https://en.wikipedia.org/wiki/Anthony_Campbell_(British_biochemist)
PseudotypingPseudotyping
... allows one to control the expression of envelope proteins. A frequently used protein is the glycoprotein G (VSV-G ... In cell culture, neutralized pseudoviruses will be prevented from infecting cells and producing the luminescent reporter gene ... Envelope proteins incorporated into the pseudovirus allow the virus to readily enter different cell types with the ... With this method, the foreign viral envelope proteins can be used to alter host tropism or increase or decrease the stability ...
https://en.wikipedia.org/wiki/Pseudotyping
StreptavidinStreptavidin
Protein tag Green NM (1975). "Avidin". Advances in Protein Chemistry. 29: 85-133. doi:10.1016/s0065-3233(08)60411-8. ISBN ... Ostojic GN, Hersam MC (June 2012). "Biomolecule-directed assembly of self-supported, nanoporous, conductive, and luminescent ... Sinclair JC, Davies KM, Vénien-Bryan C, Noble ME (July 2011). "Generation of protein lattices by fusing proteins with matching ... Protein articles without symbol, CS1: long volume value, Bacterial proteins). ...
https://en.wikipedia.org/wiki/Streptavidin
Bioluminescence imagingBioluminescence imaging
The DNA encoding the luminescent protein is incorporated into the laboratory animal either via a viral vector or by creating a ... and green fluorescent protein for continuous imaging of cell culture and animal models". Journal of Biomedical Optics. 16 (4): ...
https://en.wikipedia.org/wiki/Bioluminescence_imaging
CuttlefishCuttlefish
Furthermore, the chromatophores contain luminescent protein nanostructures in which tethered pigment granules modify light ... The blood of a cuttlefish is an unusual shade of green-blue, because it uses the copper-containing protein haemocyanin to carry ... "How Egg Case Proteins Can Protect Cuttlefish Offspring?". PLOS ONE. 10 (7): e0132836. Bibcode:2015PLoSO..1032836C. doi:10.1371/ ... oxygen instead of the red, iron-containing protein haemoglobin found in vertebrates' blood. The blood is pumped by three ...
https://en.wikipedia.org/wiki/Cuttlefish
BiofilmBiofilm
Branda SS, Chu F, Kearns DB, Losick R, Kolter R (February 2006). "A major protein component of the Bacillus subtilis biofilm ... 23 November 2016). "Salmonella biofilms using luminescent oligothiophenes". npj Biofilms and Microbiomes. 2: 16024. doi:10.1038 ... The EPS matrix consists of exopolysaccharides, proteins and nucleic acids. A large proportion of the EPS is more or less ... This means that the genes necessary for the production of proteins that work towards defending the plant against pathogens have ...
https://en.wikipedia.org/wiki/Biofilm
Eastern blotEastern blot
These include secretory proteins in prokaryotes and eukaryotes and also proteins that are intended to be incorporated in ... Olson and Eglen; Eglen, RM (2007). "beta Galactosidase complementation: A cell-based luminescent assay platform for drug ... were used to detect protein modifications. The technique showed that the antigenic proteins of the non-virulent E.muris is more ... The oxidized protein is then treated with a complex mixture, generating a new conjugate on the membrane. The membrane is then ...
https://en.wikipedia.org/wiki/Eastern_blot
John Woodland HastingsJohn Woodland Hastings
At still another level, protein phosphorylation inhibitors also influence the period of the rhythm. Other luminescent systems: ... Because the mRNAs that code for these proteins remain unchanged from day to night, the synthesis of these proteins is ... another luminescent, but heterotrophic, dinoflagellate, Noctiluca scintillans, has but a single protein, which appears to ... This work has now been expanded to other proteins in the cell. On the other hand, short pulses of inhibitors of synthesis of ...
https://en.wikipedia.org/wiki/John_Woodland_Hastings
Oplophorus-luciferin 2-monooxygenaseOplophorus-luciferin 2-monooxygenase
The protein was isolated and mutated to catalyze a bright and sustained luminescent reaction to create an engineered luciferase ... The proteins signal the enzyme for secretion in luminescence, catalyzed by the protein 19 kDa [3,4,7]. The luciferase has many ... with one cysteine in the carboxyl terminus and is distinct from proteins found in other luciferases [4]. The protein is made up ... Protein expression and purification, 56(2), 261-268. Hall, M. P., Unch, J., Binkowski, B. F., Valley, M. P., Butler, B. L., ...
https://en.wikipedia.org/wiki/Oplophorus-luciferin_2-monooxygenase
History of science and technology in JapanHistory of science and technology in Japan
Proteins and enzymes In the 1960s and 1970s, green fluorescent proteins (GFP), along with the separate luminescent protein ... He was awarded the 2008 Nobel Prize in Chemistry "for the discovery and development of the green fluorescent protein, GFP". ... a bioluminescent protein from the luminous hydromedusan, Aequorea". Journal of Cellular and Comparative Physiology. 59 (3): 223 ... he demonstrated that laser pulses could blast apart large protein molecules so that ions in gaseous form are produced. ...
https://en.wikipedia.org/wiki/History_of_science_and_technology_in_Japan
KeroplatusKeroplatus
Orfelia-type luciferin and its associated storage protein in the non-luminescent cave worm Neoditomyia sp. (Diptera: ...
https://en.wikipedia.org/wiki/Keroplatus
Organ-on-a-chipOrgan-on-a-chip
The production of urea and steady-state protein proves the viability of this device for use in high-throughput toxicity studies ... January 2010). "Bioconjugated lanthanide luminescent helicates as multilabels for lab-on-a-chip detection of cancer biomarkers ... Although PDMS is cheap, malleable, and transparent, proteins and small molecules can be absorbed by it and later leech at ... I. Molecular definition in intercalated disks of cardiomyocytes by immunoelectron microscopy of desmosomal proteins". European ...
https://en.wikipedia.org/wiki/Organ-on-a-chip
Vargula hilgendorfiiVargula hilgendorfii
Wiedenmann, Jörg (2008). "Marine proteins". In Patrick J. Walsh (ed.). Oceans and human health: risks and remedies from the ... use of the term cypridinid solves the Cypridina/Vargula dilemma for naming the constituents of the luminescent system of ...
https://en.wikipedia.org/wiki/Vargula_hilgendorfii
Plate readerPlate reader
Some of the most common assays are: ELISAs Protein and cell growth assays Protein:protein interactions Reporter assays Nucleic ... Some plate readers offer filter wheel or tunable wavelength monochromator optical systems for selecting specific luminescent ... For example, large molecules (e.g. proteins) in solution, which rotate relatively slowly because of their size, will emit ... protein and nucleic acid quantification or enzyme activity assays (i.e. in the MTT assay for cell viability). A light source ...
https://en.wikipedia.org/wiki/Plate_reader
Nicholas A. KotovNicholas A. Kotov
He established that, similarly to many proteins and other biomolecules, nanoparticles can self-organize into chains, sheets, ... "Spontaneous Organization of Single CdTe Nanoparticles into Luminescent Nanowires". Science. 297 (5579): 237-40. Bibcode:2002Sci ...
https://en.wikipedia.org/wiki/Nicholas_A._Kotov
BioluminescenceBioluminescence
Nordgren, I. K.; Tavassoli, A. (2014). "A bidirectional fluorescent two-hybrid system for monitoring protein-protein ... A cloud of luminescent material is expelled, distracting or repelling a potential predator, while the animal escapes to safety ... It produces greenish luminescent mucus which may have an anti-predator function. The marine snail Hinea brasiliana uses flashes ... Pyrosomes are colonial tunicates and each zooid has a pair of luminescent organs on either side of the inlet siphon. When ...
https://en.wikipedia.org/wiki/Bioluminescence
Index of biochemistry articlesIndex of biochemistry articles
... lipid anchored protein - lipid bilayer - lipoprotein - liquid - list of compounds - list of gene families - locus - luminescent ... protein - protein biosynthesis - Protein Data Bank - protein design - protein expression - protein folding - protein isoform - ... protein P16 - protein P34cdc2 - protein precursor - protein structure prediction - protein subunit - protein synthesis - ... proto-oncogene protein C-kit - proto-oncogene proteins c-abl - proto-oncogene proteins c-bcl-2 - Proto-oncogene proteins c-fos ...
https://en.wikipedia.org/wiki/Index_of_biochemistry_articles
DNA-functionalized quantum dotsDNA-functionalized quantum dots
In the past, green fluorescent protein (GFP) was used to track movement inside cells. However, GFP does not light up well and ... Warren CW Chan; Dustin J Maxwell; Xiaohu Gao (2002). "Luminescent quantum dots for multiplexed biological detection and imaging ... Thus, GFP prevented long term studies of protein movement. By using quantum dots, which are more stable, researchers can now ... This potentially allows researchers to understand when and where certain proteins are made. Self-assembled quantum dots form ...
https://en.wikipedia.org/wiki/DNA-functionalized_quantum_dots
Infrared Nanospectroscopy (AFM-IR)Infrared Nanospectroscopy (AFM-IR)
Middle: AFM-IR absorption at 1650 cm−1, corresponding to the amide I band associated with protein. Right: AFM-IR absorption at ... imaged by a panel of luminescent and vibrational techniques" (PDF). Analyst. 138 (19): 5627-5638. Bibcode:2013Ana...138.5627C. ... 10 nm, with a sensitivity down to the scale of molecular monolayer and single protein molecules with molecular weight down to ... The chemical and structural properties of protein determine their interactions, and thus their functions, in a wide variety of ...
https://en.wikipedia.org/wiki/Infrared_Nanospectroscopy_(AFM-IR)
List of MeSH codes (D27)List of MeSH codes (D27)
... protein kinase inhibitors MeSH D27.505.519.389.760 - protein synthesis inhibitors MeSH D27.505.519.389.936 - uncoupling agents ... luminescent agents MeSH D27.720.470.410.505.500 - fluorescent dyes MeSH D27.720.470.410.650 - radiopharmaceuticals MeSH D27.720 ...
https://en.wikipedia.org/wiki/List_of_MeSH_codes_(D27)
Bhabha Atomic Research CentreBhabha Atomic Research Centre
Studying macromolecular structures and protein-ligand interactions using biophysical techniques like X-ray crystallography, ... luminescent materials for bio-imaging, materials for light emitting devices and security applications etc. Development of new ... protein crystallography, Infrared spectroscopy, Extended X-ray absorption fine structure (EXAFS), Photoelectron spectroscopy ( ...
https://en.wikipedia.org/wiki/Bhabha_Atomic_Research_Centre
Protein tagProtein tag
"CRISPR-Mediated Tagging of Endogenous Proteins with a Luminescent Peptide". ACS Chemical Biology. 13 (2): 467-474. doi:10.1021/ ... Protein tags are peptide sequences genetically grafted onto a recombinant protein. Tags are attached to proteins for various ... a protein which binds to immobilized glutathione Green fluorescent protein-tag, a protein which is spontaneously fluorescent ... This tag is used for protein purification of recombinant proteins and its fragments. It can be used in research labs and it is ...
https://en.wikipedia.org/wiki/Protein_tag
GreenGreen
Pigments, in this case, are minerals which reflect the color green, rather that emitting it through luminescent or ... a protein that carries copper ions in chelation. The green huntsman spider is green due to the presence of bilin pigments in ...
https://en.wikipedia.org/wiki/Green
Aggregation-induced emissionAggregation-induced emission
Due to their high biocapacity and fluorescence, they can help researchers to find and mark the location of proteins. And ... "Facile Multicomponent Polymerizations toward Unconventional Luminescent Polymers with Readily Openable Small Heterocycles". ...
https://en.wikipedia.org/wiki/Aggregation-induced_emission
Irshad HussainIrshad Hussain
PVP-templated highly luminescent copper nanoclusters for sensing trinitrophenol and living cell imaging' Nanoscale, 2019. ' ... Protein-mediated synthesis, pH-induced reversible agglomeration, toxicity and cellular interaction of silver nanoparticles' ... Tan, Bien; Su, Li; Hussain, Irshad; Yan, Wei; Feng, Lingyun; Li, Yulian (2019-01-17). "PVP-templated highly luminescent copper ... Control of surface tension at liquid-liquid interfaces using nanoparticles and nanoparticle-protein complexes.' Langmuir, 2012 ...
https://en.wikipedia.org/wiki/Irshad_Hussain
Green fluorescent proteinGreen fluorescent protein
In the 1960s and 1970s, GFP, along with the separate luminescent protein aequorin (an enzyme that catalyzes the breakdown of ... Protein methods, Recombinant proteins, Cell imaging, Protein imaging, Fluorescent proteins, Bioluminescence, Cnidarian proteins ... The natural protein has 238 amino acids. Its molecular mass is 27 kD. Therefore, fusing the GFP gene to the gene of a protein ... The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to light in the blue to ...
https://en.wikipedia.org/wiki/Green_fluorescent_protein
ApoptosisApoptosis
The adenovirus E1B-55K protein and the hepatitis B virus HBx protein are examples of viral proteins that can perform such a ... Luminescent iridium complex-peptide hybrids (IPHs) have recently been designed, which mimic TRAIL and bind to death receptors ... Examples of viral Bcl-2 proteins include the Epstein-Barr virus BHRF1 protein and the adenovirus E1B 19K protein. Some viruses ... these inhibitory proteins target retinoblastoma tumor-suppressing proteins. These tumor-suppressing proteins regulate the cell ...
https://en.wikipedia.org/wiki/Apoptosis
Quantula striataQuantula striata
The snails can eat chalk for calcium, and commercially available rat food for protein. Latia neritoides, a freshwater snail ... PDF Daston M. M. & Copeland J. (1993) "The luminescent organ and sexual maturity in Dyakia striata". Malacologia 35(1): 9-19. ... abstract and PDF[permanent dead link]. Counsilman, J. J.; Ong, P. P. (1988). "Responses of the luminescent land snailDyakia ( ...
https://en.wikipedia.org/wiki/Quantula_striata
David AvnirDavid Avnir
"The near-symmetry of proteins", Proteins, 83, 722-734 (2015). doi:10.1002/prot.24706 I. Saragusti, I. Sharon, O. Katzenelson ... obtaining luminescent metals inducing magnetism in and formation of bioactive metals. The first focal point of this broad topic ... "Enzymes and Other Proteins Entrapped in Sol-Gel Materials", Chem. Mater., 6, 1605-1614 (1994).doi:10.1021/cm00046a008 S. Braun ... "Metallic Conductive Luminescent Film", ACS Nano, 13, 10826−10834 (2019). doi:10.1021/acsnano.9b06021 Sharon Marx and David ...
https://en.wikipedia.org/wiki/David_Avnir
Combinatorial chemistryCombinatorial chemistry
The beads to which the protein was attached were picked out, removed the protein from the bead then the tethered peptide was ... in the mid-nineties in the context of luminescent materials obtained by co-deposition of elements on a silicon substrate. His ... J. Peptide Protein Res., 1991, 37, 487-493. Merrifield RB, 1963 J. Am. Chem. Soc. 85, 2149. Scott, J.; Smith, G. (1990-07-27 ... The tethered peptide library was tested with a dissolved target protein. ...
https://en.wikipedia.org/wiki/Combinatorial_chemistry
Arachnocampa luminosaArachnocampa luminosa
This luminescent species resides in dark, cool and damp places such as caves and their diet is predatory, they use their bluish ... The luciferase enzyme in this species shares similarities with the protein that occurs in fireflies. However, the luciferin ... Females of the A. Luminosa are the ones that are actually luminescent but it is still unknown whether or not the female's light ...
https://en.wikipedia.org/wiki/Arachnocampa_luminosa
TRAILTRAIL
Luminescent iridium complex-peptide hybrids, which mimic TRAIL, have recently been synthesized in vitro. These artificial TRAIL ... In the field of cell biology, TNF-related apoptosis-inducing ligand (TRAIL), is a protein functioning as a ligand that induces ... Masum AA, Yokoi K, Hisamatsu Y, Naito K, Shashni B, Aoki S (September 2018). "Design and synthesis of a luminescent iridium ... It is composed of 281 amino acids and has characteristics of a type II transmembrane protein. The N-terminal cytoplasmic domain ...
https://en.wikipedia.org/wiki/TRAIL
PhosphorusPhosphorus
The main food sources for phosphorus are the same as those containing protein, although proteins do not contain phosphorus. For ... In dark surroundings, fragments are seen as luminescent spots. Promptly debride the burn if the patient's condition will permit ... As a rule, if a diet has sufficient protein and calcium, the amount of phosphorus is probably sufficient. Organic compounds of ... Cellular membranes are composed of a phospholipid matrix and proteins, typically in the form of a bilayer. Phospholipids are ...
https://en.wikipedia.org/wiki/Phosphorus
Pernicious anemiaPernicious anemia
Following its release, most B12 is absorbed by the body in the small bowel (ileum) after binding to a protein known as ... "Intrinsic factor autoantibodies by luminescent immuno-precipitation system in patients with corpus atrophic gastritis". J ... They later suggested a high-protein diet with high amounts of raw liver. This caused a rapid improvement in symptoms and a ... When foods containing B12 are eaten, the vitamin is usually bound to protein and is released by proteases released by the ...
https://en.wikipedia.org/wiki/Pernicious_anemia
COVID-19 testing - WikipediaCOVID-19 testing - Wikipedia
The result is luminescent. A chemiluminescent microparticle immunoassay uses magnetic, protein-coated microparticles. ... A plate is coated with a viral protein, such as a SARS-CoV-2 spike protein. Samples are incubated with the protein, allowing ... Antigen tests look for antigen proteins from the viral surface. In the case of a coronavirus, these are usually proteins from ... "A SARS-CoV-2 surrogate virus neutralization test based on antibody-mediated blockage of ACE2-spike protein-protein interaction ...
https://en.wikipedia.org/wiki/COVID-19_testing
Pervasive synaptic branch removal in the mammalian neuromuscular system at birthPervasive synaptic branch removal in the mammalian neuromuscular system at birth
Luminescent Proteins / genetics * Luminescent Proteins / metabolism * Mice * Mice, Transgenic * Microscopy, Confocal * ...
https://pubmed.ncbi.nlm.nih.gov/22681687/
RCSB PDB - 1KP5: Cyclic Green Fluorescent ProteinRCSB PDB - 1KP5: Cyclic Green Fluorescent Protein
Cyclic Green Fluorescent Protein. *PDB DOI: 10.2210/pdb1KP5/pdb. *Classification: LUMINESCENT PROTEIN ... Green Fluorescent Protein. A, B. 246. Aequorea victoria. Mutation(s): 20 Gene Names: GFP. ... Structure of cyclized green fluorescent protein.. Hofmann, A., Iwai, H., Hess, S., Pluckthun, A., Wlodawer, A.. (2002) Acta ... This is supported by the absence of crystal contacts in the linker-peptide region and the fact that the core of the protein ...
https://www.rcsb.org/structure/1KP5
BioenergeticsBioenergetics
To overcome this problem, we developed a luminescent pH sensor by fusing the luminescent protein Nanoluc to a uniquely designed ... A luminescent Nanoluc-GFP fusion protein enables readout of cellular pH in photosynthetic organisms. Journal of Biological ... Fluorescent sensor proteins capable of measuring in situ pH in animal cells have been reported. However, these proteins require ... Monitoring cellular redox dynamics using newly developed BRET-based redox sensor proteins. Journal of Biological Chemistry ...
https://www.jbc.org/bioenergetics?startPage=&ContribAuthorRaw=Kondo%2C%20Kumiko
Results for Innate Immunity | Abcam: antibodies, proteins, kits...Results for 'Innate Immunity' | Abcam: antibodies, proteins, kits...
Proteins and Peptides. By product type. Proteomics tools. Agonists, activators, antagonists and inhibitors. Cell lines and ...
https://www.abcam.com/products?selected.researchAreas=Immunology--Innate+Immunity
Characterization of Cu2+-binding modes in the prion protein by visible circular dichroism and multivariate curve resolution -...Characterization of Cu2+-binding modes in the prion protein by visible circular dichroism and multivariate curve resolution -...
Our study reveals the number of binding modes used in the uptake of Cu2+ by the full metal-binding region of the prion protein ... MCR-ALS is well suited to complex biochemical systems such as the prion protein which binds multiple copper ions and thus gives ... Home / KnowledgeBase Articles / Characterization of Cu2+-binding modes in the prion protein by visible circular dichroism and ... Characterization of Cu2+-binding modes in the prion protein by visible circular dichroism and multivariate curve resolution. By ...
https://jascoinc.com/knowledgebase/characterization-of-cu2-binding-modes-in-the-prion-protein-by-visible-circular-dichroism-and-multivariate-curve-resolution/
NHANES 2001-2002: C-Reactive protein (CRP), Fibrinogen, Bone Alkaline Phosphatase & Urinary N-telopeptides Data...
The bound HRP conjugate is then measured through the addition of a luminescent substrate. This signal reagent contains the ... C-reactive protein(mg/dL). English Text: C-reactive protein (mg/dL). Target: Both males and females 3 YEARS - 150 YEARS. Code ... C-reactive protein C-reactive protein is considered one of the best measures of the acute phase response to an infectious ... C-reactive protein This method quantified C-reactive protein (CRP) by latex-enhanced nephelometry. Particle-enhanced assays ...
https://wwwn.cdc.gov/Nchs/Nhanes/2001-2002/L11_B.htm
CRISPR Cas9 Knock-In Tagging | CRISPR Knock-InCRISPR Cas9 Knock-In Tagging | CRISPR Knock-In
Changes in protein abundance and details of cellular protein dynamics can be monitored in real time, in live cells at ... CRISPR-Mediated Tagging of Endogenous Proteins with a Luminescent Peptide. This ACS Chem. Biol. publication demonstrates the ... A comparison of several methods to detect and quantify any tagged protein with a simple, luminescent signal. ... Targeted Protein Degradation. Selectively targeting proteins for removal from the cell is a promising new modality for ...
https://www.promega.com/applications/small-molecule-drug-discovery/crispr-cas9-knock-in-tagging/?utm_source=promegaconnections_07_01_2020&utm_medium=social&utm_campaign=pharma_crispr
luminescent | Specktra: The online community for beautyluminescent | Specktra: The online community for beauty
Are protein bars and powders worth it? *Latest: Green Emily. *. Tuesday at 3:32 AM. ...
https://www.specktra.net/threads/luminescent.43098/
Chemistry Seminars 2017 | School of Physical and Mathematical Sciences | NTU Singapore
... firefly luminescent intermediate-based protein-protein interaction assay), which utilizes the functional complementation of two ... The luminescent proteins are entirely genetically encoded and can be engineered to generate functional probes. I herein ... Here I will introduce our novel approaches to engineer natural mono-functional proteins e.g. binding proteins such as antibody ... We found that only two or three Ds bases in the aptamers greatly affected the tight binding to target proteins. We are now ...
https://www.ntu.edu.sg/spms/about-us/chemistry/lectures-and-seminars/seminars/seminars-events-2017
Multicellular 3D Neurovascular Unit Model for Assessing Hypoxia and Neuroinflammation Induced Blood-Brain Barrier Dysfunction |...Multicellular 3D Neurovascular Unit Model for Assessing Hypoxia and Neuroinflammation Induced Blood-Brain Barrier Dysfunction |...
The expression of tight junction proteins between adjacent endothelial cells and the presence of efflux proteins prevents entry ... We evaluated the expression levels of proteins critical in BBB maintenance, basement membrane proteins, tight junction proteins ... The luminescent signal was stabilized by incubating the plate at room temperature for 10 minutes. Luminescence was measured ... Total protein levels of both multidrug resistance protein 1 (MDR-1) and aquaporin 4 (AQP4) were significantly increased in ...
https://www.nature.com/articles/s41598-020-66487-8?elqTrackId=c6110966b7b541e69110345561e951e8&error=cookies_not_supported&code=612964a8-97bb-4896-90e4-e601ee53fd10
Recording the Symphony of Cellular Signals That Drive BiologyRecording the Symphony of Cellular Signals That Drive Biology
... self-assembling proteins that act like LEGO bricks. These small proteins "clicked together," forming clusters that were ... Each cluster, which appears under the microscope as a luminescent dot, reports only one type of cellular signal. "Its like ... To see cell signaling in action, scientists typically introduce genes encoding sensors connected to fluorescent proteins. These ...
https://www.newswise.com/articles/recording-the-symphony-of-cellular-signals-that-drive-biology
NIOSHTIC-2 Search Results - Basic View
Ring Oxygen In The Tricyclo Ring System Patents and Patent Applications (Class 546/89) - Justia Patents SearchRing Oxygen In The Tricyclo Ring System Patents and Patent Applications (Class 546/89) - Justia Patents Search
... to the luminescent compounds for particular uses; to a kit comprising compounds and to a protein. The method is applicable for ... Particularly, the compounds are inhibitors of cholesterol ester transfer protein (CETP) and thus are suitable for treatment and ... Particularly, the compounds are inhibitors of cholesterol ester transfer protein (CETP) and thus are suitable for treatment and ... Abstract: Inhibitors of p67phox protein are provided herein, as well as pharmaceutical compositions and methods relating ...
https://patents.justia.com/patents-by-us-classification/546/89
Correlative cathodoluminescence electron microscopy bioimaging: towards single protein labelling with ultrastructural context -...Correlative cathodoluminescence electron microscopy bioimaging: towards single protein labelling with ultrastructural context -...
Recently, the use of correlative cathodoluminescence electron microscopy (CCLEM) imaging based on luminescent inorganic ... In this review, we discuss the opportunities of (volumetric) multi-color single protein labelling based on correlative ... Correlative cathodoluminescence electron microscopy bioimaging: towards single protein labelling with ultrastructural context ... Correlative cathodoluminescence electron microscopy bioimaging: towards single protein labelling with ultrastructural context K ...
https://pubs.rsc.org/en/content/articlelanding/2020/NR/D0NR02563A
A simple, sensitive and selective quantum-dot-based western blot method for the simultaneous detection of multiple targets from...A simple, sensitive and selective quantum-dot-based western blot method for the simultaneous detection of multiple targets from...
keywords = "Western blotting, fluorescent dyes, humans, immunoglobulin G, luminescent measurements, nerve tissue proteins, ... proteins, quantum dots, staphylococcal protein A",. author = "Gilroy, {Kathryn L.} and Cumming, {Sarah A.} and Pitt, {Andrew R ... Here, we report the straightforward generation of stable, covalent quantum dot-protein A/G bioconjugates that will be able to ... Here, we report the straightforward generation of stable, covalent quantum dot-protein A/G bioconjugates that will be able to ...
https://research.aston.ac.uk/en/publications/a-simple-sensitive-and-selective-quantum-dot-based-western-blot-m
Ataliotis P et al. (2005), XTbx1 is a transcriptional activator involved i... - Paper Ataliotis P et al. (2005), XTbx1 is a transcriptional activator involved i... - Paper
Luciferase activity was measured using a luminescent assay as described. Cotransfection of a GAL4DBD-XTbx1 fusion protein ... CAT protein levels were measured in cell lysates using a CAT-ELISA 48 hr after transfection. Transfection of 200 ng of Xbra ... A fusion protein of GAL4DBD and XTbx1 lacking the C-terminus, GAL4DBD-XTbx1-ΔCT failed to activate transcription from the GAL4- ... that XTbx1 is a transcriptional activator and that this trans-activation requires the C-terminal region of the protein. A ...
https://www.xenbase.org/entry/literature/article.do?method=display&articleId=2262
PGC1α/β Expression Predicts Therapeutic Response to Oxidative Phosphorylation Inhibition in Ovarian Cancer | Cancer Research |...PGC1α/β Expression Predicts Therapeutic Response to Oxidative Phosphorylation Inhibition in Ovarian Cancer | Cancer Research |...
Total proteins were extracted with passive lysis buffer (Promega) and quantified using the Bio-Rad Bradford protein assay. ... Intracellular ATP levels were measured with the ATP determination kit (Molecular Probes). The luminescent signal was measured ... A, PGC1α and PGC1β transcript expression and proteins in OVCAR3 and IGROV1 cells. B, Heatmap of OXPHOS-related gene expression ... A, PGC1α and PGC1β transcript expression and proteins in OVCAR3 and IGROV1 cells. B, Heatmap of OXPHOS-related gene expression ...
https://aacrjournals.org/cancerres/article/82/7/1423/694053/PGC1-Expression-Predicts-Therapeutic-Response-to
Comparative assessment of fluorescent proteins for in vivo imaging in an animal model system.
Animals, Caenorhabditis elegans, Disease Models, Animal, Luminescent Proteins, Green Fluorescent Proteins, Fluorescent Dyes, ... and we report on optimal red fluorescent proteins. These results identify ideal fluorescent proteins for imaging in vivo in C. ... or red fluorescent proteins in embryos and imaged embryos expressing different fluorescent proteins under the same conditions ... 2016). Comparative assessment of fluorescent proteins for in vivo imaging in an animal model system.. Mol Biol Cell, 27 (22), ...
https://www.repository.cam.ac.uk/handle/1810/283362
ZFIN Publication: Whitesell et al., 2014ZFIN Publication: Whitesell et al., 2014
Luminescent Proteins. *Microscopy, Confocal. *Muscle, Smooth, Vascular/embryology*. *Muscle, Smooth, Vascular/metabolism ...
https://zfin.org/ZDB-PUB-140513-416
ProtoBlock System - National DiagnosticsProtoBlock System - National Diagnostics
ProtoBlock solution contains a broad spectrum of proteins, protein analogs, detergents, and buffers which are designed to ... ProtoBlock Solution is designed to work optimally with Horseradish Peroxidase Luminescent Kit (Protoglow ECL kit, order number ... Following transfer of proteins to a membrane, it is very important to block the membrane with a good blocking solution. ... Mix well and add 20 ml of ProtoBlock Reagent B. This solution is used to block the membrane after protein transfer and to ...
https://www.nationaldiagnostics.com/product/protoblock-system/?add-to-cart=18061
Pre-Clinical Assessment of MCL-1 as a Therapeutic Target in T-Cell LymphomasPre-Clinical Assessment of MCL-1 as a Therapeutic Target in T-Cell Lymphomas
Here we characterize the BCL-2 family members by protein abundance, RNA expression, and copy number variations across a set of ... Subsequently we tested the in vitro activity of an MCL-1 inhibitor, AZD5991, using a luminescent cell viability assay. As ... In contrast, there was little correlation between drug sensitivity and either MCL-1 protein abundance or RNA expression. The in ...
https://dash.harvard.edu/handle/1/37945088
Biomedical Science, B.S.Biomedical Science, B.S.
As a biomedical science major, youll study DNA, proteins, cell biology, physiology and bioinformatics. If youre interested in ... a DNA sequencer and a fluorescent and luminescent plate reader and imager. We also have advanced cell culture facilities. ...
https://www.tarleton.edu/degrees/biomedical-science-bs/
Dual-color fluorescence lifetime correlation spectroscopy to quantify protein-protein interactions in live cell. - Nuffield...
We show that FLCS applied to dual-color EGFP and mCherry cross-correlation allows the determination of protein-protein ... laser intensities do not have to be controlled during experiment is really very helpful to study quantitatively protein ... Dual-color fluorescence correlation spectroscopy is an interesting method to quantify protein interaction in living cells. But ... Dual-color fluorescence lifetime correlation spectroscopy to quantify protein-protein interactions in live cell. Share Share ...
https://www.ndm.ox.ac.uk/publications/394789
Performance Optimization of Microvalves Based on a Microhole Array for Microfluidic ChipsPerformance Optimization of Microvalves Based on a Microhole Array for Microfluidic Chips
... microfluidic chip with a microvalve based on a microhole array is proposed in this paper for the POCT of tumor marker proteins ... and therefore the color bars appear due to the luminescent material. The quantity of the tumor marker proteins can be evaluated ... where the tumor marker proteins in the serum are labeled with the luminescent material by the immunological reaction. The ... Protein adsorption is a complex process that is affected by various factors [20], which mainly include the physical and ...
https://www.hindawi.com/journals/jamc/2020/8842890/
Why A Little Green Signaling Protein Prompted This Years No... : Neurology TodayWhy A Little Green Signaling Protein Prompted This Year's No... : Neurology Today
He is now trying to use these luminescent proteins to tag cells in humans and begin to think about ways to design cancer ... Once activated, aequorin produces blue light, but if the green fluorescent protein is nearby, the two proteins together yield a ... He searched an online database and was shocked to find a new paper in the journal Gene on the cloning of the protein - by Dr. ... Why A Little Green Signaling Protein Prompted This Years Nobel Prize in Chemistry - The Nobelists Share Their Story. * ...
https://journals.lww.com/neurotodayonline/Fulltext/2008/11060/Why_A_Little_Green_Signaling_Protein_Prompted_This.11.aspx
Luminescent ocean drifters hold keys to deep-sea animal adaptations | NSF - National Science FoundationLuminescent ocean drifters hold keys to deep-sea animal adaptations | NSF - National Science Foundation
For example, says Thuesen, "the deep sea is capable of squishing the structure of some proteins, so were investigating how ... Luminescent ocean drifters hold keys to deep-sea animal adaptations. Comb jellies, or ctenophores, link the shallows and the ... The luminescent drifters are named for the eight rows of shimmering combs that line their translucent bodies. ... While the researchers find answers, look for luminescent creatures that twirl in the shallows, flashing their comb plates like ...
https://beta.nsf.gov/news/luminescent-ocean-drifters-hold-keys-deep-sea
Creating a New Kind of Night Light: Glow-in-the-Dark Trees | Innovation| Smithsonian MagazineCreating a New Kind of Night Light: Glow-in-the-Dark Trees | Innovation| Smithsonian Magazine
Others have added a gene from the crystal jelly responsible for creating green fluorescent protein to animals such as cats and ... Traditionally, what theyve done is insert the gene for luciferase [an enzyme from a luminescent organism] along with a ... scientists have inserted human genes into plants and animals so that these hosts can produce proteins for pharmaceuticals. ... promoter [a region at the beginning of a gene that tells a cell to start transcription, the first step to producing a protein] ...
https://www.smithsonianmag.com/innovation/creating-a-new-kind-of-night-light-glow-in-the-dark-trees-9600277/
AssaysHumansNanoparticlesFluorescenceMiceAssayComplexesAntibodiesMicroscopyGeneLuminescenceOrganismTransgenicMolecularLuciferaseVectorsPolymersDesigned pH-sensPeptideMechanismsCellsExcitationMembraneReceptorsBioconjugatesSpectroscopyQuantumShineArgan oilReactive ProteinDetectionBioluminescentJellyfishAequorinCircular dichroismImmunoassaysMoleculesCellularCellIntracellularFluorescent proteinEnzymeCapsidIonsBindingPrion proteinProteomicsDetectRibosomes
Assays9
  • On the one hand, you will have to run basic assays to quantify biomolecules such as nucleic acids and proteins, or cell viability . (bmglabtech.com)
  • Here, we describe the inhibition of HIV-1 infection and the p65/p50 NF-κB phosphorylation by DING protein, analyzed by ELISA and northern-blot assays, western-blot assays, cell fractionation, and promoter-reporter assays in DING-expressing cells, using a pTet-on inducible system. (sciforschenonline.org)
  • Results from HIV-1 infection assays demonstrate a strong inhibition of HIV-1 and HIV-LTR RNA expression by DING protein, determined by p24 ELISA and by northern blot assay. (sciforschenonline.org)
  • These two proteins interact with each other, as shown by Yeast 2-hybrid (Y2H) and pull-down assays. (plantae.org)
  • BioInvenu's proprietary LinkLight™ technology offers innovative tools for cell-based protein-protein interaction assays. (bioinvenu.com)
  • Furthermore, LinkLight assay technology has a unique ability in that one protein tagged with TEV can interact with multiple proteins tagged with permuted reporters to perform multiplex assays. (bioinvenu.com)
  • The technology is also different from protein-fragment complementation assays (PCA) or enzyme-fragment complementation assays (ECA) in which fragment irreversible self-complementation could produce high background signals, especially when proteins are over-expressed or two fragments have high complementation affinity. (bioinvenu.com)
  • LinkLight™ assays can generate stable signals even from rapid transient protein-protein interactions. (bioinvenu.com)
  • Immediate & physiological relevant signals: the cell-based assays generate stable and immediate signals for either transient or stable protein-protein interactions. (bioinvenu.com)
Humans1
  • He is now trying to use these luminescent proteins to tag cells in humans and begin to think about ways to design cancer treatments using these proteins as an escort for targeted medicines. (lww.com)
Nanoparticles5
  • The application of luminescent silver nanoparticles as imaging agents for neural stem and rat basophilic leukemia cells was demonstrated. (springeropen.com)
  • It was found that towards rat basophilic leukemia cells, luminescent silver nanoparticles had a toxic effect in the silver atom concentration range of 10-100 μM. (springeropen.com)
  • The nanoparticles are much larger than the protein molecules of the cells, but many times smaller than the cells themselves. (fwf.ac.at)
  • Alfa Chemistry uses different types of natural or synthetic fluorescent substances to design and construct fluorescent coatings, including fluorescent proteins, organic fluorophores, rare earth complexes, and luminescent nanoparticles. (alfa-chemistry.com)
  • Compared with traditional organic fluorophores, luminescent nanoparticles have the following advantages: adjustable emission color and intensity, low toxicity, and stable chemical and physical properties. (alfa-chemistry.com)
Fluorescence11
  • Dual-color fluorescence lifetime correlation spectroscopy to quantify protein-protein interactions in live cell. (ox.ac.uk)
  • Dual-color fluorescence correlation spectroscopy is an interesting method to quantify protein interaction in living cells. (ox.ac.uk)
  • In 1962, Dr. Shimomura identified GFP and figured out which part of the protein is responsible for its fluorescence. (lww.com)
  • When coupled to recent technical advances in widefield fluorescence and confocal microscopy, including ultrafast low light level digital cameras and multitracking laser control systems, the green fluorescent protein and its color-shifted genetic derivatives have demonstrated invaluable service in many thousands of live-cell imaging experiments. (microscopyu.com)
  • During the isolation procedure, a second protein was observed that lacked the blue-emitting bioluminescent properties of aequorin, but was able to produce green fluorescence when illuminated with ultraviolet light. (microscopyu.com)
  • Over the next two decades, researchers determined that aequorin and the green fluorescent protein work together in the light organs of the jellyfish to convert calcium-induced luminescent signals into the green fluorescence characteristic of the species. (microscopyu.com)
  • Among the most important aspects of the green fluorescent protein to appreciate is that the entire 27 kiloDalton native peptide structure is essential to the development and maintenance of its fluorescence. (microscopyu.com)
  • Temperature dependent fluorescence data showed the strength of the dye-protein complexation to be inversely proportional to temperature and the fluorescence quenching was static in nature. (jascoinc.com)
  • The binding-induced conformational change in the protein was investigated using circular dichroism, synchronous fluorescence, 3D fluorescence and FTIR spectroscopy results. (jascoinc.com)
  • pH dependent fluorescence studies revealed the existence of electrostatic interaction between the protein and dye molecules. (jascoinc.com)
  • Some metal nanoclusters show high fluorescence and can be synthesized with protein mediation. (olemiss.edu)
Mice2
  • Tumour development and levels of associated proteins were determined in hsa_circ_102209 knockdown mice. (researchsquare.com)
  • These mice also produced larger amounts of specific proteins involved in immune responses, which contributed to the animal's increased sensitivity to pain. (elifesciences.org)
Assay9
  • Subsequently we tested the in vitro activity of an MCL-1 inhibitor, AZD5991, using a luminescent cell viability assay. (harvard.edu)
  • HRP-protein conjugates are extensively used as secondary detection reagents in ELISA, immunohistochemistry and assay development. (mobitec.com)
  • The SensoLyte® Luminescent Peroxidase Assay Kit provides highly sensitive chemiluminescent substrate to quantify peroxidase activity in solutions, in cell extracts, in live cells. (mobitec.com)
  • Methods: The amplified luminescent proximity homogeneous assay (AlphaLISA) method, which incorporates glutathione- or streptavidin-donor beads and anti-human-IgG-acceptor beads, was used to evaluate serum antibody levels in serum samples. (longdom.org)
  • In this study, qRT-PCR, Western blot, ELISA and dual luciferase assay were used to detect the cytokines, spleen index, and gene and protein expression in spleen in the exercise induced immunosuppression rat model. (alliedacademies.org)
  • We used a rapid molecular conversion assay (protein misfolding cyclic amplification) to test whether brain homogenates from specimens of classical bovine spongiform encephalopathy (BSE), atypical BSE (H-type BSE and L-type BSE), classical scrapie, atypical scrapie, and chronic wasting disease can convert normal human prion protein to the abnormal disease-associated form. (cdc.gov)
  • In terms of the reporter assay itself, biotin end-labeling of nucleic acid, fluorophore-streptavidin bioconjugation, and steric hindrances between DNA and protein molecules further contribute substantial variability to the assay. (gstp-wisc.org)
  • The LinkLight assay is not another enzyme or protein fragment complementation (EFC or PFC) methods. (bioinvenu.com)
  • The assay system can theoretically be used to find molecules that modulate protein-protein interaction or directly block protein-protein interactions. (bioinvenu.com)
Complexes2
  • Luminescent ionic transition-metal complexes and their use in light-emitting electrochemical cells are the topic of this week's Review by N. Armaroli et al. (chemistryviews.org)
  • The Highlights deal with catalysis by dinuclear gold complexes (A. Gómez-Suárez and S. P. Nolan), artificial adaptor proteins (C. Meyer and M. Köhn), and switchable catalysts (U. Lüning). (chemistryviews.org)
Antibodies8
  • Abcam: antibodies, proteins, kits. (abcam.com)
  • Western Blot immunoassay is a highly specific method of protein analysis using affinity-purified antibodies. (nationaldiagnostics.com)
  • Mix well and add 20 ml of ProtoBlock Reagent B. This solution is used to block the membrane after protein transfer and to dilute the antibodies used in the immunoassays. (nationaldiagnostics.com)
  • The fluorescent protein technique avoids the problem of purifying, tagging, and introducing labeled proteins into cells or the task of producing specific antibodies for surface or internal antigens. (microscopyu.com)
  • Antibodies can recognise certain protein structures in order to eliminate pathogens in the body. (fwf.ac.at)
  • The antibodies used in this particular case are designed to recognise a certain protein in the cell membrane of the cancer cells and bind to it. (fwf.ac.at)
  • The proteins are then transferred to a membrane where they are allowed to react to antibodies. (wholepsychiatry.com)
  • Proteins such antibodies to viral core initiation factors in the target these vaccines that viral transcription and translation initiation. (buysingulair.space)
Microscopy3
  • Recently, the use of correlative cathodoluminescence electron microscopy (CCLEM) imaging based on luminescent inorganic nanocrystals has been proposed. (rsc.org)
  • In this review, we discuss the opportunities of (volumetric) multi-color single protein labelling based on correlative cathodoluminescence electron microscopy, and its prospective impact on biomedical research in general. (rsc.org)
  • The discovery of green fluorescent protein in the early 1960s ultimately heralded a new era in cell biology by enabling investigators to apply molecular cloning methods, fusing the fluorophore moiety to a wide variety of protein and enzyme targets, in order to monitor cellular processes in living systems using optical microscopy and related methodology. (microscopyu.com)
Gene13
  • Fluorescent protein tags are fundamental tools used to visualize gene products and analyze their dynamics in vivo. (cam.ac.uk)
  • He searched an online database and was shocked to find a new paper in the journal Gene on the cloning of the protein - by Dr. Prasher. (lww.com)
  • Although the gene for green fluorescent protein was first cloned in 1992, the significant potential as a molecular probe was not realized until several years later when fusion products were used to track gene expression in bacteria and nematodes. (microscopyu.com)
  • A cassette containing the structural gene for the FACS-adapted green fluorescent protein mutant 2 (GFPmut2), expressed from the 5' and 3' flanking sequences of the circumsporozoite (CS) protein gene, was integrated and expressed at the endogenous CS locus. (ox.ac.uk)
  • Reporter vectors containing reporter genes are usually used to assess the transfection efficiency of a target gene into a cell, as well as the protein expression of the target gene. (amerigoscientific.com)
  • In addition, reporter vectors can be used to monitor other processes such as recombination events, gene targeting, RNA processing, protein secretion pathways, and signal transduction pathways in cells. (amerigoscientific.com)
  • Novel plant DING proteins (full-length 38 kDa p38SJ, and 27 kDa p27SJ) exhibit phosphatase activity and modulate HIV-1 gene transcription. (sciforschenonline.org)
  • Electronic cigarette smoke reduces ribosomal protein gene expression to impair protein synthesis in primary human airway epithelial cells. (harvard.edu)
  • Using gene set enrichment analysis, we found that high ACSL4 expression was frequently associated with the oxidative stress pathway, especially ferroptosis-related proteins. (biomedcentral.com)
  • Most cases of human prion disease are apparently spontaneously occurring (sporadic CJD [sCJD]) or are associated with mutations in the human prion protein gene, designated PRNP (genetic CJD, Gerstmann-Sträussler-Scheinker disease, or fatal familial insomnia). (cdc.gov)
  • Regulatory elements controlling the expression of the NfdnaK2 gene were identified by fusing putative promoters to luminescent reporter gene luxAB in Nostoc sp. (plantae.org)
  • The adenoviral vector gene delivery system is a range of ready-to-use recombinant adenoviral preparations, each of which contains the gene encoding a protein target fused to EGFP or emerald FP, or a response element controlling the expression of nitroreductase. (genomeweb.com)
  • Recently, we demonstrated that the antipsychotic drugs clozapine and haloperidol stimulate lipogenic gene expression in cultured glioma cells through activation of the sterol regulatory element-binding protein (SREBP) transcription factors. (biomedcentral.com)
Luminescence1
  • Considering the pharmacological applications and the development of luminescent sensors and optoelectronic devices, NMR studies in combination with other techniques, such as theoretical and luminescence studies are also performed. (uc.pt)
Organism1
  • What if a bioluminescent protein could cast its light in an organism that was already transparent? (lww.com)
Transgenic2
  • We generated transgenic Caenorhabditis elegans strains expressing green, yellow, or red fluorescent proteins in embryos and imaged embryos expressing different fluorescent proteins under the same conditions for direct comparison. (cam.ac.uk)
  • Additionally, after high light exposure, the D1 protein turned over much faster in the transgenic strain, which also showed higher levels of FtsH compared to WT. (plantae.org)
Molecular3
  • The molecular interaction between hemoglobin (HHb), the major human heme protein, and the acridine dyes acridine orange (AO) and 9-aminoacridine (9AA) was studied by various spectroscopic, calorimetric and molecular modeling techniques. (jascoinc.com)
  • Molecular chaperones such as Heat shock protein (Hsp) 70 and Hsp40 guide this process (Hartl and Hayer-Hartl, 2009). (plantae.org)
  • provide an in depth molecular analysis of DnaJ-DnaK proteins and their role in PSII repair in N. flagelliforme . (plantae.org)
Luciferase6
  • In vivo imaging of cells tagged with light-emitting probes, such as firefly luciferase or fluorescent proteins, is a powerful technology that enables a wide range of biological studies in small research animals. (perkinelmer.com)
  • Dr. Katarina Bohm, Marketing Manager of Promega: "Our luciferase vectors translate cellular changes into a luminescent signal. (analytica-world.com)
  • Plasmids encoding the dif ferent virus proteins have been cotransfected using the reporter plas mid pISRE 54 CAT likewise like a plasmid driving the constitu tive expression of rey luciferase. (sirnalibrary.com)
  • A protein A is linked to a Tobacco Etch Virus (TEV) protease and a protein B is linked to a permuted luciferase (pLuc). (bioinvenu.com)
  • Upon interaction between protein A and B, inactive pLuc is cleaved, the cleaved luciferase fragments are spontaneously refolded, driven by fragment self-complementation affinity, and active luciferase is reconstituted. (bioinvenu.com)
  • Reporter proteins that generate a fluorescent, colorimetric, luminescent, or cell survival readout can be permuted to substitute the permuted luciferase in the LinkLight™ technology. (bioinvenu.com)
Vectors5
  • The HeLa cells were co-transfected with sub-cellular localization vectors fused to cyan ( mTurquoise ) and yellow ( mVenus ) fluorescent protein coding sequences (Golgi complex and the nucleus, respectively), as well as the "Fruit" protein, mCherry, targeting the mitochondrial network. (microscopyu.com)
  • Green fluorescent protein, and its mutated allelic forms, blue, cyan, and yellow fluorescent proteins are used to construct fluorescent chimeric proteins that can be expressed in living cells, tissues, and entire organisms, after transfection with the engineered vectors. (microscopyu.com)
  • Amerigo Scientific offers flexible, convenient, and robust reporter vectors and dual-reporter vectors for assessing the efficiency of transfection in cells, targeting of different cell locations, obtaining cell lines with reporter proteins, or other applications. (amerigoscientific.com)
  • The production of green fluorescent protein (GFP) in Methylobacterium extorquens was studied by creating four different constructs using pJB3KmD, pRK310 and pVK101 vectors, as well as pLac and soluble methane monooxygenase (sMMO) promoters. (canada.ca)
  • UI - 20564155LA - engRN - 0 (Culture Media)RN - 0 (Genetic Vectors)RN - 0 (Luminescent Proteins)RN - 0 (Plasmids)RN - 0 (Recombinant Proteins)RN - 147336-22-9 (green fluorescent protein)RN - 7440-50-8 (Copper)RN - EC 1.13. (canada.ca)
Polymers1
  • We aim at the development of new polymeric materials including cellulose-based polymers functionalization, conducting and luminescent sensing polymers and bio-hydrogels for drug delivery and water remediation. (uc.pt)
Designed pH-sens1
  • To overcome this problem, we developed a luminescent pH sensor by fusing the luminescent protein Nanoluc to a uniquely designed pH-sensitive GFP variant protein. (jbc.org)
Peptide3
  • This is supported by the absence of crystal contacts in the linker-peptide region and the fact that the core of the protein exhibits a very similar conformation to that known from other GFP structures, thereby not implicating any constraints arising from the presence of the artificial linker. (rcsb.org)
  • What is unique to the fluorescent protein is that the location of this peptide triplet resides in the center of a remarkably stable barrel structure consisting of 11 beta -sheets folded into a tube. (microscopyu.com)
  • The protein array method was used for the initial screening, and peptide arrays were used to identify epitope sites. (longdom.org)
Mechanisms2
  • KEY WORDS: REDD1, mTOR, G-protein-coupled receptors, Bioluminescence resonance energy transfer, Ca 2+ /calmodulin signaling pathway, Plasma membrane translocation INTRODUCTION One of the key mechanisms of signal transduction is a change in intracellular localization of signaling effectors. (vdocuments.mx)
  • Structural analysis of JAK2 kinase in complicated with NS 018 Phosphorylation from the activation loop is amongst the most common mechanisms for regulating protein kinase action, and it prospects towards the relocation of an Asp Phe Gly motif positioned adjacent for the N terminus with the A loop. (sirnalibrary.com)
Cells19
  • Fluorescent sensor proteins capable of measuring in situ pH in animal cells have been reported. (jbc.org)
  • The restoration of the apoptotic cascade in tumor cells has long been recognized as a promising way to treat cancer but the major members of this protein family, BCL2, MCL1, and BCL-xL have long remained elusive targets decades long for drug discovery. (ntu.edu.sg)
  • The expression of tight junction proteins between adjacent endothelial cells and the presence of efflux proteins prevents entry of foreign substances into the brain parenchyma. (nature.com)
  • Most quantitative comparisons of the brightness and photostability of different fluorescent proteins have been made in vitro, removed from biological variables that govern their performance in cells or organisms. (cam.ac.uk)
  • We show that FLCS applied to dual-color EGFP and mCherry cross-correlation allows the determination of protein-protein interactions in living cells without the need of spectral bleed through calibration. (ox.ac.uk)
  • He is one of three scientists to win the 2008 Nobel Prize in Chemistry for their work in developing green fluorescent protein (GFP) to track changes in cells or organisms. (lww.com)
  • With the rapid evolution of fluorescent protein technology, the utility of this genetically encoded fluorophore for a wide spectrum of applications beyond the simple tracking of tagged biomolecules in living cells is now becoming fully appreciated. (microscopyu.com)
  • Illustrated in Figure 1 are two examples of multiple fluorescent protein labeling in living cells using fusion products targeted at sub-cellular (organelle) locations. (microscopyu.com)
  • We experimentally demonstrate ten-color super-resolution imaging in vitro on synthetic DNA structures as well as four-color two-dimensional imaging and three-color 3D imaging of proteins in fixed cells. (mpg.de)
  • Messenger RNA (mRNA) based vaccines use lab-created mRNA that provides cells with instructions for making protein antigens and triggers an adaptive immune response. (abnova.com)
  • Promega Corp. announced that it has signed an agreement with Leica Microsystems to enable fluorescent imaging of proteins in live cells. (analytica-world.com)
  • GATA binding protein 3 (GTA3) is a Th2-type specific transcription factor that is only expressed in Th2 cells and is essential in the Th2 differentiation [ 7 , 8 ]. (alliedacademies.org)
  • Recently, we have shown that insulin or IGF-I stimulation of Mvt1 cells leads to the upregulation of several mRNA transcripts, associated with cell proliferation and migration, such as Cyclin D1 and the transcription factor ETS2, as well as to the downregulation of transcriptional repressor, high mobility group (HMG) box-containing protein 1 (HBP1) ( 12 ). (frontiersin.org)
  • We now present the successful identification of REDD1 during such a protein translocation screening procedure in living cells. (vdocuments.mx)
  • In a Minireview, A. Rutkowska and C. Schultz discuss methods and reagents for the visualization and induction of dynamic protein-protein interactions in living cells . (chemistryviews.org)
  • The 2KNS4B protein from WNV has become demonstrated to avoid STAT1 phos phorylation in IFN treated cells. (sirnalibrary.com)
  • LinkLight™ technology provides simple, robust, economic, and sensitive methods to detect molecules modulating protein-protein interactions in live cells with immediate and stable signals. (bioinvenu.com)
  • In addition to long running symposia on batteries, semiconductors, fuel cells, fullerenes, and luminescent materials, the Seattle meeting will also explore newer areas such as materials recycling, data science for modeling and design, consumer products, and flexible electronics. (electrochem.org)
  • Using far red/near-infrared light-emitting diode devices (called High Emissivity Aluminiferous Luminescent Substrate, or HEALS in this case) has been shown to release long wavelength energy in the form of photons that stimulate cells to aid in healing. (findmeacure.com)
Excitation2
  • However, these proteins require an excitation laser for pH measurement that may affect photosynthetic performance and induce autofluorescence from chlorophyll. (jbc.org)
  • The core itself is a spherical "quantum dot," an extraordinarily small semiconductor that is luminescent upon optical excitation. (lbl.gov)
Membrane6
  • Following transfer of proteins to a membrane, it is very important to block the membrane with a good blocking solution. (nationaldiagnostics.com)
  • The cell consists of a permeable cell membrane, DNA, protein factories called ribosomes, and a protective outer cell wall. (si.edu)
  • Also, in pharmacology, they have been used to screen compound libraries for new drugs by high-content screening, for example in the G-protein- coupled receptor (GPCR) field, using plasma membrane translocation of the general effector b -arrestin2 as a read-out of GPCR activation (Hudson et al. (vdocuments.mx)
  • GPCR s are membrane-localized proteins that play an important role in cell signaling. (moleculardevices.com)
  • Photosystem II (PSII) is a thylakoid-membrane integral protein complex of the electron transport chain that particularly is sensitive to photodamage under drought. (plantae.org)
  • The technology can be applied to a broad range of protein-protein interactions by engineering appropriate fusion protein partners, such as cell membrane and cytoplasmic protein interactions, membrane, and membrane protein interactions, cytoplasmic protein interactions, nuclear protein interactions, and cell-cell interaction through membrane protein interactions. (bioinvenu.com)
Receptors3
  • G protein-coupled receptors (GPCRs) represent one of the most important therapeutic targets in drug discovery research. (moleculardevices.com)
  • Recently three orphan G-protein coupled receptors, RAIG1, GPRC5B and GPRC5C, with homology to members of family C (metabotropic glutamate receptor-like) have been identified. (ku.dk)
  • Using the protein sequences of these receptors as queries we identified overlapping expressed sequence tags which were predicted to encode an additional subtype. (ku.dk)
Bioconjugates1
  • Here, we report the straightforward generation of stable, covalent quantum dot-protein A/G bioconjugates that will be able to bind to almost any IgG antibody, and therefore can be used in many applications. (aston.ac.uk)
Spectroscopy1
  • Near-field spectroscopy of the quantum constituents of a luminescent system. (janelia.org)
Quantum2
  • There have been several attempts to make graphene-based quantum dots with specific electronic and luminescent properties using chemical breakdown or e-beam lithography of graphene layers," said Ajayan, Rice's Benjamin M. and Mary Greenwood Anderson Professor of Mechanical Engineering and Materials Science and of Chemistry. (wordpress.com)
  • Luminescent semiconductor nanocrystals (quantum dots) provide a promising alternative to traditional fluorescent labels. (gstp-wisc.org)
Shine3
  • Indulge in rich, fortifying keratin proteins that reinforce each strand, increasing elasticity to resist breakage caused by brushing and styling, while argan oil conditions and smoothes creating a luminescent shine. (makeupandbeauty.com)
  • This Blend Helps To Resist Breakage Caused By Brushing And Styling, While Enhancing A Luminescent Shine. (kunooz.com)
  • This unique formulation contains enhancing colour preserving nutrients to help revitalize while providing luminescent shine and eliminating flyaways. (veganizedvanity.com)
Argan oil1
  • Indulge In This Fortifying Blend With Keratin Proteins And Argan Oil. (kunooz.com)
Reactive Protein2
  • C-reactive protein is considered one of the best measures of the acute phase response to an infectious disease or other cause of tissue damage and inflammation. (cdc.gov)
  • This method quantified C- reactive protein (CRP) by latex-enhanced nephelometry. (cdc.gov)
Detection2
  • Detection of the virus is usually done either by looking for the virus's inner RNA, or pieces of protein on the outside of the virus. (wikipedia.org)
  • A chemical reaction produces light (called chemo-luminescent detection) which is then detected by photographic film. (wholepsychiatry.com)
Bioluminescent2
  • Osamu Shimomura and Frank Johnson, working at the Friday Harbor Laboratories of the University of Washington in 1961, first isolated a calcium-dependent bioluminescent protein from the Aequorea victoria jellyfish, which they named aequorin . (microscopyu.com)
  • These reporters include enzymes giving new enzymatic abilities to the cell, existing enzymes but with higher thermal stabilities, products secreted by the cell that can be easily measured in the surrounding media, fluorescent proteins, and bioluminescent proteins. (amerigoscientific.com)
Jellyfish1
  • He found that calcium ions activate a jellyfish protein called aequorin. (lww.com)
Aequorin2
  • Once activated, aequorin produces blue light, but if the green fluorescent protein is nearby, the two proteins together yield a bright green signal. (lww.com)
  • Once Ca 2+ ions are bound to the three Ca 2+ -binding sites in aequorin, the protein is converted into an oxygenase. (biomedcentral.com)
Circular dichroism1
  • Visible circular dichroism (CD) spectra from the copper(II) titration of the metal-binding region of the prion protein, residues 57-98, were analyzed using the self-modeling curve resolution method multivariate curve resolution-alternating least squares (MCR-ALS). (jascoinc.com)
Immunoassays1
  • Multiplexed immunoassays for proteins using magnetic luminescent nanoparticle s for internal calibration. (cdc.gov)
Molecules1
  • Certain sugar molecules can interact selectively with bacterial proteins, and the researchers plan to harness these interaction to make fluorescent materials which glow at first, darkening when they become compromised by bacteria, allowing clinicians to react faster to potential infections before they become a serious risk to patient health. (byrneresearch.com)
Cellular5
  • Each cluster, which appears under the microscope as a luminescent dot, reports only one type of cellular signal. (newswise.com)
  • We show a true biological validation using a four-colour multiplexed western blot against a complex cell lysate background, and have significantly improved previously reported non-specific binding of the Qdots to cellular proteins. (aston.ac.uk)
  • However, cellular mechanics are ultimately generated by structures at the scale of a single protein, and current techniques cannot map forces with nanoscale spatial resolution. (lbl.gov)
  • The prion hypothesis posits that the posttranslational conformational conversion of a host's normal cellular prion protein (PrP C ) by the abnormal form of the prion protein (PrP Sc ) is the fundamental event in prion disease pathogenesis and that PrP Sc itself constitutes the infectious agent. (cdc.gov)
  • One interesting point incorporate the life cycle of RNA viruses is that empire have evolved several strategies for expressing their own proteins inside the host is by modulating cellular transcription and translation. (buysingulair.space)
Cell5
  • To see cell signaling in action, scientists typically introduce genes encoding sensors connected to fluorescent proteins. (newswise.com)
  • These small proteins "clicked together," forming clusters that were randomly scattered across the cell like little islands. (newswise.com)
  • Here we characterize the BCL-2 family members by protein abundance, RNA expression, and copy number variations across a set of 21 T-NHL cell lines and 5 patient-derived xenograft models. (harvard.edu)
  • Providing our customers with innovative bioanalytical solutions for protein and cell biology for over 30 years. (moleculardevices.com)
  • When a receptor is activated by a ligand, the conformation of the receptor is modified, activating G-proteins inside the cell. (moleculardevices.com)
Intracellular4
  • They trigger intracellular signaling pathways by the activation of heterotrimeric G-proteins. (vdocuments.mx)
  • 2009). We reasoned that screening a library of fluorescently modified proteins of unknown function for any changes in their intracellular localization upon GPCR activation might allow us to discover new protein components of the GPCR signaling pathways. (vdocuments.mx)
  • An active G-protein has the potential to induce various cascades of intracellular messengers, including calcium. (moleculardevices.com)
  • Imaging intracellular fluorescent proteins at nanometer resolution. (janelia.org)
Fluorescent protein8
  • Crystals of cyclic green fluorescent protein (cGFP) engineered by the previously reported split intein technology [Iwai et al. (rcsb.org)
  • Recent advances in genome editing have expedited the precise insertion of fluorescent protein tags into the genomes of diverse organisms. (cam.ac.uk)
  • To address the gap, we quantitatively assessed fluorescent protein properties in vivo in an animal model system. (cam.ac.uk)
  • This year's Nobel laureates in chemistry discuss their journey toward discovering green fluorescent protein. (lww.com)
  • Due to this property, the protein was eventually christened with the unceremonious name of green fluorescent protein ( GFP ). (microscopyu.com)
  • Since these early studies, green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors that are broadly referred to as fluorescent proteins. (microscopyu.com)
  • loop_ _audit_author.name _audit_author.pdbx_ordinal 'Shu, X.' 1 'Remington, S.J.' 2 # _citation.id primary _citation.title 'Ultrafast excited-state dynamics in the green fluorescent protein variant S65T/H148D. (rcsb.org)
  • Examples of these labels include many well-known organic fluorophores and naturally occurring chromophores such as green or red fluorescent protein and phycoerytherin. (gstp-wisc.org)
Enzyme1
  • LinkLight technology overcomes the significant drawback of enzyme or protein fragment spontaneous self-complementation. (bioinvenu.com)
Capsid1
  • Inhibition of viral protein of the capsid protein synthesis and translate genes in maturation. (buysingulair.space)
Ions2
  • MCR-ALS is well suited to complex biochemical systems such as the prion protein which binds multiple copper ions and thus gives rise to titration data consisting of several pure component spectra with overlapped or superimposed absorption bands. (jascoinc.com)
  • When mixed in solution, the free Au3+ ions from HAuCl4 form clusters by attaching to the many cysteine side chains of the protein. (olemiss.edu)
Binding3
  • Our study reveals the number of binding modes used in the uptake of Cu2+ by the full metal-binding region of the prion protein and their relative concentration profiles throughout the titration. (jascoinc.com)
  • These variants have in common a higher transmissibility, and an accumulation of a high number of mutations in the spike protein, especially within the amino terminal domain (NTD) and the receptor binding domain (RBD). (abnova.com)
  • These include the binding of a substance to one of the skin's proteins and a resulting stress reaction. (analytica-world.com)
Prion protein3
  • The fundamental event in prion disease pathogenesis is thought to be the seeded conversion of normal prion protein to its pathologic isoform. (cdc.gov)
  • None of the tested prion isolates from diseased animals were as efficient as classical BSE in converting human prion protein. (cdc.gov)
  • However, in the case of chronic wasting disease, there was no absolute barrier to conversion of the human prion protein. (cdc.gov)
Proteomics1
  • A range of techniques, mostly focused on detecting interactions with GPCRs themselves, including proteomics and two- hybrid screening, have been used to identify the regulatory proteins within the diverse GPCR signaling pathways (Daulat et al. (vdocuments.mx)
Detect3
  • Fluorescent or luminescent reporters have been adopted more widely because they are more sensitive, easier to detect and quantify. (amerigoscientific.com)
  • The qRT-PCR and Western Blot were used to detect the mRNA and protein change of GATA-3 in spleen tissue. (alliedacademies.org)
  • It can also be used to detect proteins in native PAGE gels after an additional SDS incubation step. (moisteane.com.cn)
Ribosomes1

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