A photoprotein isolated from the bioluminescent jellyfish Aequorea. It emits visible light by an intramolecular reaction when a trace amount of calcium ion is added. The light-emitting moiety in the bioluminescence reaction is believed to be 2-amino-3-benzyl-5-(p-hydroxyphenyl)pyrazine (AF-350).
Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.
Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.
A dye used to stain proteins in electrophoretic techniques. It is used interchangeably with its acid form.
Exclusive legal rights or privileges applied to inventions, plants, etc.
Elements with partially filled d orbitals. They constitute groups 3-12 of the periodic table of elements.
A novel composition, device, or process, independently conceived de novo or derived from a pre-existing model.
Property, such as patents, trademarks, and copyright, that results from creative effort. The Patent and Copyright Clause (Art. 1, Sec. 8, cl. 8) of the United States Constitution provides for promoting the progress of science and useful arts by securing for limited times to authors and inventors, the exclusive right to their respective writings and discoveries. (From Black's Law Dictionary, 5th ed, p1014)
The study of microorganisms living in a variety of environments (air, soil, water, etc.) and their pathogenic relationship to other organisms including man.
The study of microorganisms such as fungi, bacteria, algae, archaea, and viruses.
A publication issued at stated, more or less regular, intervals.
Techniques used in microbiology.
A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Methods for determining interaction between PROTEINS.
Graphs representing sets of measurable, non-covalent physical contacts with specific PROTEINS in living organisms or in cells.
Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
Protein modules with conserved ligand-binding surfaces which mediate specific interaction functions in SIGNAL TRANSDUCTION PATHWAYS and the specific BINDING SITES of their cognate protein LIGANDS.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein.
A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors.
An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.
Agents that inhibit PROTEIN KINASES.
It is a form of protection provided by law. In the United States this protection is granted to authors of original works of authorship, including literary, dramatic, musical, artistic, and certain other intellectual works. This protection is available to both published and unpublished works. (from Circular of the United States Copyright Office, 6/30/2008)
Transparent, tasteless crystals found in nature as agate, amethyst, chalcedony, cristobalite, flint, sand, QUARTZ, and tridymite. The compound is insoluble in water or acids except hydrofluoric acid.
Cancers or tumors of the LARYNX or any of its parts: the GLOTTIS; EPIGLOTTIS; LARYNGEAL CARTILAGES; LARYNGEAL MUSCLES; and VOCAL CORDS.
Trans-acting transcription factors produced by retroviruses such as HIV. They are nuclear proteins whose expression is required for viral replication. The tat protein stimulates LONG TERMINAL REPEAT-driven RNA synthesis for both viral regulatory and viral structural proteins. tat stands for trans-activation of transcription.
Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.
The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
Proteins encoded by the TAT GENES of the HUMAN IMMUNODEFICIENCY VIRUS.
A nitrocellulose solution in ether and alcohol. Collodion has a wide range of uses in industry including applications in the manufacture of photographic film, in fibers, in lacquers, and in engraving and lithography. In medicine it is used as a drug solvent and a wound sealant.
Narrow pieces of material impregnated or covered with a substance used to produce a chemical reaction. The strips are used in detecting, measuring, producing, etc., other substances. (From Dorland, 28th ed)
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Artificially produced membranes, such as semipermeable membranes used in artificial kidney dialysis (RENAL DIALYSIS), monomolecular and bimolecular membranes used as models to simulate biological CELL MEMBRANES. These membranes are also used in the process of GUIDED TISSUE REGENERATION.
A process of separating particulate matter from a fluid, such as air or a liquid, by passing the fluid carrier through a medium that will not pass the particulates. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The sum of the weight of all the atoms in a molecule.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
Electron microscopy involving rapid freezing of the samples. The imaging of frozen-hydrated molecules and organelles permits the best possible resolution closest to the living state, free of chemical fixatives or stains.
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.
The portion of an interactive computer program that issues messages to and receives commands from a user.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Sequential operating programs and data which instruct the functioning of a digital computer.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
The naturally occurring or experimentally induced replacement of one or more AMINO ACIDS in a protein with another. If a functionally equivalent amino acid is substituted, the protein may retain wild-type activity. Substitution may also diminish, enhance, or eliminate protein function. Experimentally induced substitution is often used to study enzyme activities and binding site properties.
A species of nematode that is widely used in biological, biochemical, and genetic studies.
Proteins from the nematode species CAENORHABDITIS ELEGANS. The proteins from this species are the subject of scientific interest in the area of multicellular organism MORPHOGENESIS.
A genus of small free-living nematodes. Two species, CAENORHABDITIS ELEGANS and C. briggsae are much used in studies of genetics, development, aging, muscle chemistry, and neuroanatomy.
The functional hereditary units of HELMINTHS.
Proteins found in any species of helminth.
Deoxyribonucleic acid that makes up the genetic material of helminths.
A class of unsegmented helminths with fundamental bilateral symmetry and secondary triradiate symmetry of the oral and esophageal structures. Many species are parasites.

Mitochondrial depolarization accompanies cytochrome c release during apoptosis in PC6 cells. (1/8843)

Cytochrome c is released from mitochondria into the cytosol in cells undergoing apoptosis. The temporal relationship between cytochrome c release and loss of mitochondrial membrane potential was monitored by laser-scanning confocal microscopy in single living pheochromocytoma-6 cells undergoing apoptosis induced by staurosporine. Mitochondrial membrane potential monitored by tetramethylrhodamine methyl ester decreased abruptly in individual cells from 2 to 7 h after treatment with staurosporine. Depolarization was accompanied by cytochrome c release documented by release of transfected green fluorescent protein-tagged cytochrome c in these cells. The results show that mitochondrial depolarization accompanies cytochrome c release in pheochromocytoma-6 cells undergoing apoptosis.  (+info)

The dually acylated NH2-terminal domain of gi1alpha is sufficient to target a green fluorescent protein reporter to caveolin-enriched plasma membrane domains. Palmitoylation of caveolin-1 is required for the recognition of dually acylated g-protein alpha subunits in vivo. (2/8843)

Here we investigate the molecular mechanisms that govern the targeting of G-protein alpha subunits to the plasma membrane. For this purpose, we used Gi1alpha as a model dually acylated G-protein. We fused full-length Gi1alpha or its extreme NH2-terminal domain (residues 1-32 or 1-122) to green fluorescent protein (GFP) and analyzed the subcellular localization of these fusion proteins. We show that the first 32 amino acids of Gi1alpha are sufficient to target GFP to caveolin-enriched domains of the plasma membrane in vivo, as demonstrated by co-fractionation and co-immunoprecipitation with caveolin-1. Interestingly, when dual acylation of this 32-amino acid domain was blocked by specific point mutations (G2A or C3S), the resulting GFP fusion proteins were localized to the cytoplasm and excluded from caveolin-rich regions. The myristoylated but nonpalmitoylated (C3S) chimera only partially partitioned into caveolin-containing fractions. However, both nonacylated GFP fusions (G2A and C3S) no longer co-immunoprecipitated with caveolin-1. Taken together, these results indicate that lipid modification of the NH2-terminal of Gi1alpha is essential for targeting to its correct destination and interaction with caveolin-1. Also, a caveolin-1 mutant lacking all three palmitoylation sites (C133S, C143S, and C156S) was unable to co-immunoprecipitate these dually acylated GFP-G-protein fusions. Thus, dual acylation of the NH2-terminal domain of Gi1alpha and palmitoylation of caveolin-1 are both required to stabilize and perhaps regulate this reciprocal interaction at the plasma membrane in vivo. Our results provide the first demonstration of a functional role for caveolin-1 palmitoylation in its interaction with signaling molecules.  (+info)

Properties of filament-bound myosin light chain kinase. (3/8843)

Myosin light chain kinase binds to actin-containing filaments from cells with a greater affinity than to F-actin. However, it is not known if this binding in cells is regulated by Ca2+/calmodulin as it is with F-actin. Therefore, the binding properties of the kinase to stress fibers were examined in smooth muscle-derived A7r5 cells. Full-length myosin light chain kinase or a truncation mutant lacking residues 2-142 was expressed as chimeras containing green fluorescent protein at the C terminus. In intact cells, the full-length kinase bound to stress fibers, whereas the truncated kinase showed diffuse fluorescence in the cytoplasm. After permeabilization with saponin, the fluorescence from the truncated kinase disappeared, whereas the fluorescence of the full-length kinase was retained on stress fibers. Measurements of fluorescence intensities and fluorescence recovery after photobleaching of the full-length myosin light chain kinase in saponin-permeable cells showed that Ca2+/calmodulin did not dissociate the kinase from these filaments. However, the filament-bound kinase was sufficient for Ca2+-dependent phosphorylation of myosin regulatory light chain and contraction of stress fibers. Thus, dissociation of myosin light chain kinase from actin-containing thin filaments is not necessary for phosphorylation of myosin light chain in thick filaments. We note that the distance between the N terminus and the catalytic core of the kinase is sufficient to span the distance between thin and thick filaments.  (+info)

A fluorescent orthotopic bone metastasis model of human prostate cancer. (4/8843)

Here, we report a fluorescent spontaneous bone metastatic model of human prostate cancer developed by surgical orthotopic implantation of green fluorescent protein (GFP)-expressing prostate cancer tissue. Human prostate cancer PC-3 cells were transduced with the pLEIN expression retroviral vector containing the enhanced GFP and neomycin resistance genes. Stable GFP high-expression PC-3 clones were selected in vitro with G418, which were then combined and injected s.c. in nude mice. For metastasis studies, fragments of a single highly fluorescent s.c. growing tumor were implanted by surgical orthotopic implantation in the prostate of a series of nude mice. Subsequent micrometastases and metastases were visualized by GFP fluorescence throughout the skeleton, including the skull, rib, pelvis, femur, and tibia The central nervous system, including the brain and spinal cord, was also involved with tumor, as visualized by GFP fluorescence. Systemic organs, including the lung, plural membrane, liver, kidney, and adrenal gland, also had fluorescent metastases. The metastasis pattern in this model reflects the bone and other metastatic sites of human prostate cancer. Thus, this model should be very useful for the study and development of treatment for metastatic androgen-independent prostate cancer.  (+info)

Role of cytokine signaling molecules in erythroid differentiation of mouse fetal liver hematopoietic cells: functional analysis of signaling molecules by retrovirus-mediated expression. (5/8843)

Erythropoietin (EPO) and its cell surface receptor (EPOR) play a central role in proliferation, differentiation, and survival of erythroid progenitors. Signals induced by EPO have been studied extensively by using erythroid as well as nonerythroid cell lines, and various controversial results have been reported as to the role of signaling molecules in erythroid differentiation. Here we describe a novel approach to analyze the EPO signaling by using primary mouse fetal liver hematopoietic cells to avoid possible artifacts due to established cell lines. Our strategy is based on high-titer retrovirus vectors with a bicistronic expression system consisting of an internal ribosome entry site (IRES) and green fluorescent protein (GFP). By placing the cDNA for a signaling molecule in front of IRES-GFP, virus-infected cells can be viably sorted by fluorescence-activated cell sorter, and the effect of expression of the signaling molecule can be assessed. By using this system, expression of cell-survival genes such as Bcl-2 and Bcl-XL was found to enhance erythroid colony formation from colony-forming unit-erythroid (CFU-E) in response to EPO. However, their expression was not sufficient for erythroid colony formation from CFU-E alone, indicating that EPO induces signals for erythroid differentiation. To examine the role of EPOR tyrosine residues in erythroid differentiation, we introduced a chimeric EGFR-EPOR receptor, which has the extracellular domain of the EGF receptor and the intracellular domain of the EPOR, as well as a mutant EGFR-EPOR in which all the cytoplasmic tyrosine residues are replaced with phenylalanine, and found that tyrosine residues of EPOR are essential for erythroid colony formation from CFU-E. We further analyzed the function of the downstream signaling molecules by expressing modified signaling molecules and found that both JAK2/STAT5 and Ras, two major signaling pathways activated by EPOR, are involved in full erythroid differentiation.  (+info)

Nuclear translocation of green fluorescent protein-nuclear factor kappaB with a distinct lag time in living cells. (6/8843)

A highly fluorescent mutant form of the green fluorescent protein (GFP) has been fused to the human nuclear factor kappaB (NF-kappaB) p50 and p105 (p50/IkappaB gamma), a precursor protein of NF-kappaB p50. GFP-p50 and GFP-p105 were expressed in monkey COS-7 cells and human HeLa cells. Translocation of these chimeric proteins was observed by confocal laser scanning microscopy. GFP-p50 (without IkappaB gamma) in the transfected cells resided in the nucleus. On the other hand, GFP-p105 (GFP-p50 with IkappaB gamma) localized only in the cytoplasm before stimulation and translocated to the nucleus with stimulant specificity similar to that of native NF-kappaB/IkappaB. In addition, the translocation of NF-kappaB to the nucleus had a distinct lag time (a quiescent time) in the target cells. The lag time lasted 10-20 min after stimulation with hydrogen peroxide or tumor necrosis factor alpha. It was suggested that this might be due to the existence of a limiting step where NF-kappaB is released from NF-kappaB/IkappaB by the proteasome.  (+info)

Microtubule-dependent plus- and minus end-directed motilities are competing processes for nuclear targeting of adenovirus. (7/8843)

Adenovirus (Ad) enters target cells by receptor-mediated endocytosis, escapes to the cytosol, and then delivers its DNA genome into the nucleus. Here we analyzed the trafficking of fluorophore-tagged viruses in HeLa and TC7 cells by time-lapse microscopy. Our results show that native or taxol-stabilized microtubules (MTs) support alternating minus- and plus end-directed movements of cytosolic virus with elementary speeds up to 2.6 micrometer/s. No directed movement was observed in nocodazole-treated cells. Switching between plus- and minus end-directed elementary speeds at frequencies up to 1 Hz was observed in the periphery and near the MT organizing center (MTOC) after recovery from nocodazole treatment. MT-dependent motilities allowed virus accumulation near the MTOC at population speeds of 1-10 micrometer/min, depending on the cell type. Overexpression of p50/dynamitin, which is known to affect dynein-dependent minus end-directed vesicular transport, significantly reduced the extent and the frequency of minus end-directed migration of cytosolic virus, and increased the frequency, but not the extent of plus end-directed motility. The data imply that a single cytosolic Ad particle engages with two types of MT-dependent motor activities, the minus end- directed cytoplasmic dynein and an unknown plus end- directed activity.  (+info)

Estimation of the number of alpha-helical and beta-strand segments in proteins using circular dichroism spectroscopy. (8/8843)

A simple approach to estimate the number of alpha-helical and beta-strand segments from protein circular dichroism spectra is described. The alpha-helix and beta-sheet conformations in globular protein structures, assigned by DSSP and STRIDE algorithms, were divided into regular and distorted fractions by considering a certain number of terminal residues in a given alpha-helix or beta-strand segment to be distorted. The resulting secondary structure fractions for 29 reference proteins were used in the analyses of circular dichroism spectra by the SELCON method. From the performance indices of the analyses, we determined that, on an average, four residues per alpha-helix and two residues per beta-strand may be considered distorted in proteins. The number of alpha-helical and beta-strand segments and their average length in a given protein were estimated from the fraction of distorted alpha-helix and beta-strand conformations determined from the analysis of circular dichroism spectra. The statistical test for the reference protein set shows the high reliability of such a classification of protein secondary structure. The method was used to analyze the circular dichroism spectra of four additional proteins and the predicted structural characteristics agree with the crystal structure data.  (+info)

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Initially, we were faced with the decision of how to efficiently construct and screen libraries of DsRed mutants such that we could direct the evolution of tetrameric DsRed toward a mRFP. Ultimately the best solution was to take the semirational approach of breaking the dimer interfaces in a stepwise fashion (first AB then AC) and undertaking a directed evolution strategy to rescue the red fluorescence. This combination of targeted and random mutagenesis successfully directed the evolution of DsRed from the poorly fluorescent dimer T1-I125R to the monomeric mRFP1 in eight generations. In retrospect, breaking up the tetramer was not the barrier to the discovery of mRFP1; the challenge was to find the correct combination of many mutations to rescue the red fluorescence in the crippled dimers and monomers.. There are likely several mechanisms by which mutations in dimer2 and mRFP1 have contributed to rescuing the red fluorescence. Although one mechanism probably involves improving the folding ...
Initially, we were faced with the decision of how to efficiently construct and screen libraries of DsRed mutants such that we could direct the evolution of tetrameric DsRed toward a mRFP. Ultimately the best solution was to take the semirational approach of breaking the dimer interfaces in a stepwise fashion (first AB then AC) and undertaking a directed evolution strategy to rescue the red fluorescence. This combination of targeted and random mutagenesis successfully directed the evolution of DsRed from the poorly fluorescent dimer T1-I125R to the monomeric mRFP1 in eight generations. In retrospect, breaking up the tetramer was not the barrier to the discovery of mRFP1; the challenge was to find the correct combination of many mutations to rescue the red fluorescence in the crippled dimers and monomers.. There are likely several mechanisms by which mutations in dimer2 and mRFP1 have contributed to rescuing the red fluorescence. Although one mechanism probably involves improving the folding ...
Green-to-red photoconversion is a reaction that occurs in a limited number of fluorescent proteins and that is currently mechanistically debated. In this contribution, we report on our investigation of the photoconvertible fluorescent protein Dendra2 by employing a combination of pump-probe, up-conversion and single photon timing spectroscopic techniques. Our findings indicate that upon excitation of the neutral green state an excited state proton transfer proceeds with a time constant of 3.4 ps between the neutral green and the anionic green states. In concentrated solution we detected resonance energy transfer (25 ps time constant) between green and red monomers. The time-resolved emission spectra suggest also the formation of a super-red species, first observed for DsRed (a red fluorescent protein from the corallimorph species Discosoma) and consistent with peculiar structural details present in both proteins.
Author(s): Shaner, Nathan Christopher | Abstract: Fluorescent proteins are intrinsically fluorescent, genetically encodable tags that can be expressed in many heterologous organisms. Originally cloned from jellyfish and corals, these proteins and their engineered derivatives have become ubiquitous tools in molecular and cell biology. While wild-type fluorescent proteins sometimes possess sufficiently beneficial properties to be used unmodified, many applications require improvements in brightness or photostability, reduction of oligomerization, or other specific properties that require additional engineering of the wild-type protein. This dissertation presents experiments drawn from the entire spectrum of fluorescent protein science, from the cloning of novel wild-type fluorescent proteins to the engineering of wavelength-shifted, photostable, and photoactivatable variants of existing fluorescent proteins. The previously engineered monomeric red fluorescent protein, mRFP1, was engineered through a
Read pSAT vectors: a modular series of plasmids for autofluorescent protein tagging and expression of multiple genes in plants, Plant Molecular Biology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
mKate2 is the next generation of monomeric far-red fluorescent protein TagFP635 (mKate) [Shcherbo et al., 2007; Shcherbo et al., 2009]. Possessing fluorescence with excitation/emission maxima at 588 and 633 nm, mKate2 is almost 3-fold brighter than TagFP635 and is 10-fold brighter than mPlum at the physiological pH = 7.5. Within the optical window optimal for light penetration in living tissues, calculated brightness of mKate2 is at least 2-fold higher compared to any monomeric fluorescent protein reported to date. mKate2 is characterized by complete and fast chromophore maturation at 37°C with maturation half-time ,20 min (versus 40 min for mCherry). It is more photostable under both widefield and confocal illumination than other monomeric far-red proteins, including TagFP635, mRaspberry and mPlum. The high brightness, far-red emission spectrum, excellent pH resistance and photostability, coupled with low toxicity demonstrated in transgenic Xenopus laevis embryos, make mKate2 a superior ...
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Citrine ... the stone of wealth. Premium grade citrine varies from deep yellow to a wonderful yellow-orange hue. Very bright with minimal brown tones. Clarity is eye clean. Fine quality cutting. Almost all citrine today is created by heat-treating amethyst.
Supplementary MaterialsSupplementary Details Supplementary information srep00688-s1. results in the conformational dynamics from the RFP chromophore. The genetically-encoded fluorescent proteins (FPs) are effective equipment for imaging in biology1. The prototypical green fluorescent proteins (GFP) in the jellyfish includes an 11-stranded -barrel encircling the 4-(p-hydroxybenzylidene)imidazolidin-5-one chromophore, which is normally produced autocatalytically1. The crimson fluorescent proteins (DsRed) from coral isomerization from the chromophore in the thrilled state, regarding rotation around imidazolinone exocyclic connection (I-bond)6. Furthermore to I-bond isomerization, the rotation around phenyl (P-) connection may also be extremely efficient (occasionally barrierless and on picosecond timescale) in the isolated chromophore7,8,9,10,11,12,13,14. In mFruits and DsRed, fast reversible bleaching seen in mass tests15,16, fluorescence relationship17,18,19,20, and one molecule spectroscopy20,21 ...
Simple and easy to engineer metal-sensing molecules that are capable of differentiating metal ions and producing metal-specific signals are highly desirable. Metal ions affect the thermal stability of proteins by increasing or decreasing their resistance to unfolding. This work illustrates a new strategy for designing bivalent fluorescent fusion proteins capable of differentiating metal ions in solution through their distinct effects on a proteins thermal stability. A new dual purpose metal sensor was developed consisting of biotin protein ligase (BirA) from B. pseudomallei (Bp) fused to green fluorescent protein (GFP). When coupled with differential scanning fluorimetry of GFP-tagged proteins (DSF-GTP) for signal-transduction detection, Bp BirA-GFP yields distinct protein unfolding signatures with Zn(II) and Cu(II) ions in aqueous solutions. The limit of detection of the system is ∼1 μM for both metal species. The system can be used in a variety of high-throughput assay formats including ...
Plasmid AAV-FLEX-rev-ChR2-tdtomato from Dr. Scott Sternsons lab contains the insert Channelrhodopsin 2-tdtomato and is published in J Neurosci. 2008 Jul 9. 28(28):7025-30. This plasmid is available through Addgene.
Plasmid 20XUAS IVS CsChrimson tdtomato_tr from Dr. Vivek Jayaramans lab contains the insert Chrimson_tdtomato_trafficked and is published in Nat Methods. 2014 Mar;11(3):338-46. Epub 2014 Feb 9. This plasmid is available through Addgene.
This X-linked targeted knock-in strain co-marks cells expressing the Foxp3 (forkhead box P3) gene with monomeric red fluorescent protein (mRFP). RFP expression faithfully marks gene expression in lymphocytes. This strain may be helpful in studies of Foxp3-expressing regulatory T cells.
Author: Reiländer, Helmut et al.; Genre: Journal Article; Published in Print: 1996-02-06; Title: Functional Expression of the Aequorea victoria Green Fluorescent Protein in Insect Cells Using the Baculovirus Expression System
Structural investigations on green fluorescent protein variants and the adenylyl cyclase associated protein [Elektronische Ressource] / Dorota Ksiazek : Technische Universität München Institut für Organische Chemie und Biochemie Max-Planck-Institut für Biochemie Abteilung Strukturforschung Biologische NMR-Arbeitsgruppe Structural Investigations on Green Fluorescent Protein Variants and the Adenylyl Cyclase-Associated Protein Dorota Ksiazek Vollständiger Abdruck der von der Fakultät für Chemie der Technischen Universität München zur
TY - JOUR. T1 - Green fluorescent protein mutant as label in homogeneous assays for biomolecules. AU - Deo, Sapna K.. AU - Daunert, Sylvia. PY - 2001/2/1. Y1 - 2001/2/1. N2 - The green fluorescent protein (GFP) and its mutants have been extensively used to study various cellular processes and, more recently, as labels in binding assays. We have employed a mutant of GFP, an enhanced GFP (EGFP), in the development of homogeneous assays for biotin and cortisol. To demonstrate the feasibility of using EGFP as a label with different kinds of binders in the development of homogeneous assays, we employed the biotin-avidin and an antigen-antibody as the binding pairs. Biotin and cortisol were chemically conjugated to EGFP. A quenching of fluorescence intensity of EGFP was observed upon binding of avidin to the EGFP-biotin conjugate. The percentage fluorescence quenching observed decreased as the concentration of free biotin in the sample increased due to the fewer binding sites on avidin available for ...
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
TY - JOUR. T1 - Engineering ESPT pathways based on structural analysis of LSSmKate red fluorescent proteins with large stokes shift. AU - Piatkevich, Kiryl D.. AU - Malashkevich, Vladimir N.. AU - Almo, Steven C.. AU - Verkhusha, Vladislav. PY - 2010/8/11. Y1 - 2010/8/11. N2 - LSSmKate1 and LSSmKate2 are monomeric red fluorescent proteins (RFPs) with large Stokes shifts (LSSs), which allows for efficient separation of absorbance and emission maxima, as well as for excitation with conventional two-photon laser sources. These LSSmKates differ by a single amino acid substitution at position 160 and exhibit absorbance maxima around 460 nm, corresponding to a neutral DsRed-like chromophore. However, excitation at 460 nm leads to fluorescence emission above 600 nm. Structures of LSSmKate1 and LSSmKate2, determined at resolutions of 2.0 and 1.5, respectively, revealed that the predominant DsRed-chromophore configurations are cis for LSSmKate1 but trans for LSSmKate2. Crystallographic and mutagenesis ...
TY - JOUR. T1 - FMDV replicons encoding green fluorescent protein are replication competent. AU - Tulloch, Fiona. AU - Pathania, Uday. AU - Luke, Garry A.. AU - Nicholson, John. AU - Stonehouse, Nicola J.. AU - Rowlands, David J.. AU - Jackson, Terry. AU - Tuthill, Toby. AU - Haas, Juergen. AU - Lamond, Angus I.. AU - Ryan, Martin D.. PY - 2014/12/1. Y1 - 2014/12/1. N2 - The study of replication of viruses that require high bio-secure facilities can be accomplished with less stringent containment using non-infectious replicon systems. The FMDV replicon system (pT7rep) reported by Mclnerney et al. (2000) was modified by the replacement of sequences encoding chloramphenicol acetyl-transferase (CAT) with those encoding a functional (L pro) linked to a bi-functional fluorescent/antibiotic resistance fusion protein (green fluorescent protein/puromycin resistance, [GFP-PAC]). Cells were transfected with replicon-derived transcript RNA and GFP fluorescence quantified. Replication of transcript RNAs ...
Supplementary Materialsoncotarget-09-6518-s001. This demonstrates the significant potential of alveolar type II cells in orchestrating the process of metastasis, rendering it as one of the target cell types of the lung of therapeutic importance in human NSCLC. expression is usually replaced with reddish fluorescent protein (DsRed*MST) expression in tissues expressing Cre recombinase. We utilized transgenic mice in which the human SPC (Sftpc) gene promoter is used expressing the invert tetracycline transactivator (rtTA) hence placing the appearance of Cre-recombinase (CRE) beneath the conditional control of doxycycline. Appearance of Cre was utilized to completely label cells with Crimson fluorescent proteins (DsRed) in alveolar type II cells. Distinctive lines of transgenic mice that exhibit rtTA beneath the control of the individual surfactant-associated proteins C (Sftpc/SPC) gene promoter had been bred VE-821 enzyme inhibitor to TetO-Cre mice and reporter mice (LacZ/DsRed) creating triple ...
Current methods for determining ambient redox potential in cells are labor-intensive and generally require destruction of tissue. This precludes single cell or real time studies of changes in redox poise that result from metabolic processes or environmental influences. By substitution of surface-exposed residues on the Aequorea victoria green fluorescent protein (GFP) with cysteines in appropriate positions to form disulfide bonds, reduction-oxidation-sensitive GFPs (roGFPs) have been created. roGFPs have two fluorescence excitation maxima at about 400 and 490 nm and display rapid and reversible ratiometric changes in fluorescence in response to changes in ambient redox potential in vitro and in vivo. Crystal structure analyses of reduced and oxidized crystals of roGFP2 at 2.0- and 1.9-A resolution, respectively, reveal in the oxidized state a highly strained disulfide and localized main chain structural changes that presumably account for the state-dependent spectral changes. roGFP1 has been ...
Aequoria victoria green fluorescent protein (GFP) is a revolutionary molecular biology tool because of its spontaneous peptide backbone cyclization and chromophore formation from residues Ser65, Tyr66, and Gly67. Here we use structure-based design, comprehensive targeted mutagenesis, and high-resolution crystallography to probe the significant functional role of conserved Arg96 (R96) in chromophore maturation. The R96M GFP variant, in which the R96M side chain is similar in volume but lacks the R96 positive charge, exhibits dramatically slower chromophore maturation kinetics (from hours to months). Comparison of the precyclized conformation of the chromophore-forming residues with the mature R96M chromophore reveals a similar Y66 conformer, contrary to the large Y66 conformational change previously defined in the slowly maturing R96A variant [Barondeau, D. P., Putnam, C. D., Kassmann, C. J., Tainer, J. A., and Getzoff, E. D. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 12111-12116]. Comprehensive ...
References for The Development of New FPs 15. Shaner , N C, Patterson, G H, & Davidson, M W. (2007). Advances in fluorescent protein technology . Journal of Cell Science, 120. PMID: 18057027 16. Shaner, N C, Steinbach, P A & Tsien, R Y. (2005). A guide to choosing fluorescent proteins. Nature Methods, 2, PMID: 16299475 17. Ai, H, Shaner, N C, Cheng, Z, Tsien, R Y & Campbell, R E. (2007). Exploration of new chromophore structures leads to the identification of improved blue fluorescent proteins. Biochemistry, 46, PMID: 17444659 18. Kremers, G J, Goedhart, J, van den Heuvel, D J, Gerritsen, H C. & Gadella, T W J. (2007). Improved green and blue fluorescent proteins for expression in bacteria and mammalian cells. Biochemistry, 46, PMID: 17323929 19. Cubitt, A B., Heim, R, Adams, S R, Boyd, A E, Gross, L A & Tsien, R Y. (1995). Understanding, improving and using green fluorescent proteins. Trends Biochemical Science, 20, PMID: 8578587 20. Rizzo, M A, Springer, G H, Granada, B & Piston, D W. (2004). ...
References for The Development of New FPs 15. Shaner , N C, Patterson, G H, & Davidson, M W. (2007). Advances in fluorescent protein technology . Journal of Cell Science, 120. PMID: 18057027 16. Shaner, N C, Steinbach, P A & Tsien, R Y. (2005). A guide to choosing fluorescent proteins. Nature Methods, 2, PMID: 16299475 17. Ai, H, Shaner, N C, Cheng, Z, Tsien, R Y & Campbell, R E. (2007). Exploration of new chromophore structures leads to the identification of improved blue fluorescent proteins. Biochemistry, 46, PMID: 17444659 18. Kremers, G J, Goedhart, J, van den Heuvel, D J, Gerritsen, H C. & Gadella, T W J. (2007). Improved green and blue fluorescent proteins for expression in bacteria and mammalian cells. Biochemistry, 46, PMID: 17323929 19. Cubitt, A B., Heim, R, Adams, S R, Boyd, A E, Gross, L A & Tsien, R Y. (1995). Understanding, improving and using green fluorescent proteins. Trends Biochemical Science, 20, PMID: 8578587 20. Rizzo, M A, Springer, G H, Granada, B & Piston, D W. (2004). ...
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
We present recent data on dynamic imaging of Rac1 activity in live T-cells. Förster resonance energy transfer between enhanced green and monomeric red fluorescent protein pairs which form part of a biosensor molecule provides a metric of this activity. Microscopy is performed using a multi-functional high-content screening instrument using fluorescence anisotropy to provide a means of monitoring protein-protein activity with high temporal resolution. Specifically, the response of T-cells upon interaction of a cell surface receptor with an antibody coated multi-well chamber was measured. We observed dynamic changes in the activity of the biosensor molecules with a time resolution that is difficult to achieve with traditional methodologies for observing Förster resonance energy transfer (fluorescence lifetime imaging using single photon counting or frequency domain techniques) and without spectral corrections that are normally required for intensity based methodologies.
Intensive searches for novel green fluorescent protein (GFP)-like fluorescent proteins have identified more than 150 distinct genes that, together with its mutants, cover the excitation range from 380 to 600 nanometers (nm) and the emission range from 440 to 650 nm (see table below). Despite spectral diversity, a family of GFP-like proteins possesses common significant structural, biochemical and photophysical features. Many of these spectroscopically active proteins are developed to commercially available genetically-encoded fluorescent probes. In comparison to other natural pigments and fluorophores, GFP-like proteins stand out because they form internal chromophores without requiring accessory cofactors, external enzymatic catalysis or substrates other than molecular oxygen. It gives GFP-like proteins many advantages including that chromophore formation is possible in live organisms, tissues or cells while maintaining their integrity as well as molecular, organelle and tissue targeting and ...
The recent emergence of an autofluorescent protein, the green fluorescent protein (GFP), has opened the door for the convenient use of intact living cells and organisms as experimental systems in fields ranging from cell biology to biomedicine. We present an overview of some of the major applications of GFP, namely its use in protein tagging and in monitoring gene expression as well as its potential in a variety of biological screens.. ...
Fluorescent proteins are likely one of the most famous research tools derived from bioprospecting. Examples include dsRed as well as GFP and its many derivatives, which have been utilized throughout biological research. Interestingly, these fluorescent proteins are finding new purpose in medicine as visual guides during surgery. Before tumorectomy, a mouse with internal tumors is injected with a recombinant form of GFP, which is targeted to and accumulates on the cells of blood vessels. During surgical removal of the tumor, the introduced GFP provides a surgeon with a strong visual queue of nearby blood vessels greatly reducing the risk of blood vessel lacerations. DNA and RNA polymerases are the workhorses of modern biotechnology. Almost every aspect of modern biological research depends upon nucleic acid polymerases in one way or another. Recombinant cloning techniques, Sanger sequencing, and qPCR cover a few of the most common uses. These examples also highlight the shared importance of ...
TY - JOUR. T1 - DNA sequence-enabled reassembly of the green fluorescent protein. AU - Stains, Cliff I.. AU - Porter, Jason R.. AU - Ooi, Aik T.. AU - Segal, David J.. AU - Ghosh, Indraneel. PY - 2005/8/10. Y1 - 2005/8/10. N2 - We describe a general methodology for the direct detection of DNA by the design of a split-protein system that reassembles to form an active complex only in the presence of a targeted DNA sequence. This approach, called SEquence Enabled Reassembly (SEER) of proteins, combines the ability to rationally dissect proteins to construct oligomerization-dependent protein reassembly systems and the availability of DNA binding Cys2-His2 zinc-finger motifs for the recognition of specific DNA sequences. We demonstrate the feasibility of the SEER approach utilizing the split green fluorescent protein appended to appropriate zinc fingers, such that chromophore formation is only catalyzed in the presence of DNA sequences that incorporate binding sites for both zinc fingers.. AB - We ...
TY - JOUR. T1 - Structural characterization of the photoswitchable fluorescent protein Dronpa-C62S. AU - Nam, Ki Hyun. AU - Kwon, Oh Yeun. AU - Sugiyama, Kanako. AU - Lee, Won Ho. AU - Kim, Young Kwan. AU - Song, Hyun Kyu. AU - Kim, Eunice Eunkyung. AU - Park, Sam Yong. AU - Jeon, Hyesung. AU - Hwang, Kwang Yeon. N1 - Funding Information: We thank Dr. H.S. Lee and his staff for assistance during data collection at beamline 4A of Pohang Light Source, Korea. H.J. is supported by Grant M10420010001-04N2001-00110 from MOST, Korea and by the Molecular Imaging Program at the Korea Institute of Science and Technology. K.Y.H. is supported by the Functional Proteomics Center, 21C Frontier Program of the Korea Ministry of Science and Technology. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.. PY - 2007/3/23. Y1 - 2007/3/23. N2 - The photoswitching behavior of green fluorescent proteins (GFPs) or GFP-like proteins is increasingly recognized as a new technique for optical marking. Recently, ...
The green fluorescent protein (GFP) is the most commonly used reporter protein for monitoring gene expression and protein localization in a variety of living and fixed cells, including not only prokaryotes, but eukaryotes also, e. EGFP inhibited both cell nest and growth development, and activated cell loss of life in Ku80-lacking hamster cells, i.y., xrs-6 cells. In addition, Ku80 attenuated EGFP-induced cytotoxicity in the xrs-6 cells. No EGFP-induced cytotoxicity was noticed in the NHEJ primary proteins XRCC4-lacking hamster cells, i.y., XR-1 cells. Furthermore, Substantially enhanced X-ray-induced cytotoxicity in the xrs-6 cells EGFP. These outcomes recommend that Ku80 has a essential function in the story NHEJ-independent protection system against EGFP-induced cytotoxicity. Extreme care should end up being used in taking into consideration of the potential impact by the tension response system, specifically, the Ku80-reliant reduction system of EGFP-induced cytotoxicity, getting turned on, ...
Mechanics of living mammalian cytoplasm 1.Overview The cytoplasm of living mammalian cells is a crowded, yet dynamic environment(1). It provides the key physical environment to the cellular factory and all intracellular physiological processes from transcription, translation, to protein binding and folding. Therefore, understanding the fundamental physical nature of the cytoplasm is critical to understanding the basic physiology of cells. Moreover, there are continuous intracellular movements that are vital for cell function, such as transport of vesicles and other organelles. While biological motors and other enzymatic processes provide key driving forces for these activities, the mechanical behavior of the cytoplasm are crucial for determining the mechanical resistance that cellular compartments experience. Both the active driving force and appropriate mechanical environment are critical for shaping the living cellular machinery. However, while the force that molecular motors generate both ...
The culture of human osteosarcoma cells featured in this section was transfected with a pDsRed-Mitochondria plasmid subcellular localization vector, thus localizing a red fluorescent protein tag to the intracellular mitochondrial network.
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Photoswitchable protein IrisFP molecule. Computer model showing the structure of the reversibly photoswitchable green to red fluorescent protein IrisFP. - Stock Image C035/6331
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We report for the first time the rela time non-invasive kinetic analysis of three steps in the NF-κB signalling pathway; IκBα degradation, p65 translocation and NF-κB-dependent transcription. We have used these tools to investigate the link between the kinetics of the NF-κB pathway, the levels of NF-κB and IκB proteins in cell compartments, and the resulting timing of transcription.. We showed that both the p65-EGFP and p65-dsRed fluorescent fusion proteins gave rise to the nuclear translocation in response to TNFα treatment, which is characteristic of the functional endogenous protein. Ding et al. previously reported an endogenous p65 nuclear translocation half time of 7-8 minutes in HeLa cells following TNFα stimulation ( Ding et al., 1998). In comparison, we obtained a longer half time of 19±2.9 minutes for nuclear translocation of p65-EGFP in singly transfected cells ( Fig. 1A) in agreement with other studies using a p65-EGFP fluorescent fusion protein and stimulation with IL-1β ( ...
The collection of specimens illustrated in this section demonstrates the effectiveness of the Nikon YFP HYQ filter combination with a series of cell lines containing a chimeric EYFP plasmid vector that expresses a fluorescent fusion protein targeted at the intracellular Golgi apparatus.
The collection of specimens illustrated in this section demonstrates the effectiveness of the Nikon YFP HYQ filter combination with a series of cell lines containing a chimeric EYFP plasmid vector that expresses a fluorescent fusion protein targeted at the cellular endosomal network.
pKatushka2S-N is a mammalian expression vector encoding far-red fluorescent protein Katushka2S (see reporter description). The vector allows generation of fusions to the Katushka2S N-terminus and expression of Katushka2S fusions or Katushka2S alone in eukaryotic (mammalian) cells. Katushka2S codon usage is optimized for high expression in mammalian cells (humanized) [Haas et al., 1996]. To increase mRNA translation efficiency, Kozak consensus translation initiation site is generated upstream of the Katushka2S coding sequence [Kozak, 1987]. Multiple cloning site (MCS) is located between PCMV IE and Katushka2S coding sequence. The vector backbone contains immediate early promoter of cytomegalovirus (PCMV IE) for protein expression, SV40 origin for replication in mammalian cells expressing SV40 T-antigen, pUC origin of replication for propagation in E. coli, and f1 origin for single-stranded DNA production. SV40 polyadenylation signals (SV40 poly A) direct proper processing of the 3-end of the ...
Spectral imaging and linear unmixing has become an important tool in confocal and widefield fluorescence microscopy to discriminate between fluorophores having overlapping spectral characteristics.
Since the discovery of the green fluorescent protein gene, fluorescent proteins have impacted molecular cell biology. This protocol...
Congratulations to Dr Mark Fricker for his work on the paper ATP sensing in living plant cells reveals tissue gradients and stress dynamics of energy physiology that was published open access on the eLIFE website.. Read the paper here: https://doi.org/10.7554/eLife.26770. Press release: https://www.uni-bonn.de/news/178-2017. ...
In search of localised membrane protein assembly centres in bacteria. PspA (Phage-Shock Protein A) is a widespread bacterial protein known to be important for preserving membrane integrity under environmental stress conditions. The related Vipp1 protein is found in cyanobacteria (and plant chloroplasts) and seems to play a crucial role in the biogenesis of photosynthetic membranes. In both case, the mechanism of action of the protein is enigmatic. We have visualised both proteins in vivo using fluorescence microscopy and fluorescent protein tags, and found that they form clusters near to the membrane under the conditions when they are likely to be physiologically active. Combined with biochemical identification of interaction partners under these conditions, this suggests a novel hypothesis: that both these proteins may help to organise assembly centres for the rapid and localised production of membrane and secreted proteins. A key prediction of this hypothesis is that specific mRNA molecules ...
The result is luminescent. A chemiluminescent microparticle immunoassay uses magnetic, protein-coated microparticles. ... A plate is coated with a viral protein, such as a SARS-CoV-2 spike protein. Samples are incubated with the protein, allowing ... Antigen tests look for antigen proteins from the viral surface. In the case of a coronavirus, these are usually proteins from ... "A SARS-CoV-2 surrogate virus neutralization test (sVNT) based on antibody-mediated blockage of ACE2-spike (RBD) protein-protein ...
"Luminescent Eel Muscles Fluorescent Protein Revolution into Clinic." Huffington Post, June 18, 2013. "Mending Broken Hearts: ...
A way around this problem is with luminescent tags. These tags are quantum dots attached to proteins that penetrate cell ... These devices that are built with nanowires to detect cancer proteins; each nanowire detector is primed to be sensitive to a ... Binding agents such as proteins, antibiotics, or synthetic ligands are covalently linked to the particle surface. These binding ... able to detect proteins and other biomarkers left behind by cancer cells, could enable the detection and diagnosis of cancer in ...
Turro, C; Fu, PK; Bradley, PM (2003). "Lanthanide ions as luminescent probes of proteins and nucleic acids". Metal Ions in ... polyaminocarboxylates as luminescent sensors in time-resolved luminescent (TRL) immunoassays. Optimization of analytical ... The luminescent probe may for instance serve to localize the MRI contrast agent. This has helped to visualize the delivery of ... LRET was also used to study the interaction of the proteins dystrophin and actin in muscle cells. Dystrophin is present in the ...
Permyakov, Eugene A.. [Luminescent Spectroscopy of Proteins], CRC Press, 1993. Fluorescence lifetimes and dynamic quenching. ...
"Cloning and sequence analysis of cDNA for the luminescent protein aequorin". Proc. Natl. Acad. Sci. U.S.A. 82 (10): 3154-58. ... "Prediction of EF-hand calcium-binding proteins and analysis of bacterial EF-hand proteins". Proteins. 65 (3): 643-55. doi: ... Notably, the protein contains three EF hand motifs that function as binding sites for Ca2+ ions. The protein is a member of the ... In the animals, the protein occurs together with the green fluorescent protein to produce green light by resonant energy ...
Bioluminescence imaging - a technique for studying laboratory animals using luminescent protein. Calcium imaging - determining ...
A number of atomically precise luminescent clusters have been made in proteins and their growth involves inter-protein metal ... "Understanding the Evolution of Luminescent Gold Quantum Clusters in Protein Templates". ACS Nano. 5 (11): 8816-8827. doi: ... Combining luminescent atomically precise clusters with mesoflowers and nanofibres, he developed sensors at sub-zeptomole levels ... 4. Noble metal clusters in protein templates, T. Pradeep, A. Baksi and P. L. Xavier in Functional nanometer-sized clusters of ...
Recently, chimeras of RLuc have been developed and demonstrated to be the brightest luminescent proteins to date, and have ... "Luminescent proteins for high-speed single-cell and whole-body imaging". Nature Communications. 3: 1262. Bibcode:2012NatCo... ... Protein Engineering. 5 (3): 197-211. doi:10.1093/protein/5.3.197. PMID 1409539. Loening AM, Fenn TD, Wu AM, Gambhir SS ( ... and is coupled with a closely interacting green fluorescent protein (RrGFP), and a Ca++ activated luciferin binding protein ( ...
"Luminescent quantum clusters of gold in transferrin family protein, lactoferrin exhibiting FRET" (PDF). Nanoscale. 2 (12): 2769 ... The fraction of protein extracted from milk, contains 3.3% RNA, but, the protein preferably binds to double-stranded DNA rather ... Occurrence of iron-containing red protein in bovine milk was reported as early as in 1939; however, the protein could not be ... optical absorption spectra and presence of two iron atoms per protein molecule. The protein was extracted from milk, contained ...
Lamture, JB; Wensel, TG (1995). "Intensely luminescent immunoreactive conjugates of proteins and dipicolinate-based polymeric ... phycoerythrin or green fluorescent protein). Alternatively, specific or general proteins, nucleic acids, lipids or small ... Other proteins are fluorescent but require a fluorophore cofactor, and hence can only be used in vitro; these are often found ... Several fluorescent protein exist in nature[citation needed], but the most important one as a research tool is Green ...
... used reporter genes that induce visually identifiable characteristics usually involve fluorescent and luminescent proteins. ... The mRNA is then translated into protein. It is important that both proteins be able to properly fold into their active ... Nordgren, I. K.; Tavassoli, A (2014). "A bidirectional fluorescent two-hybrid system for monitoring protein-protein ... A more complex use of reporter genes on a large scale is in two-hybrid screening, which aims to identify proteins that natively ...
Martin Chalfie (professor, United States): for developing new methods for bioluminescent analysis using GFP luminescent protein ...
Amyloid beta (Aβ) is a small protein, most often 40 or 42 amino acids in length, that is released from a longer parent protein ... Klingstedt T; Nilsson KPR (2012). "Luminescent conjugated poly- and oligo-thiophenes: optical ligands for spectral assignment ... The misfolded proteins stick to one another, eventually stacking together to form protofibrils that twist together to make the ... The normal function of Aβ is not certain, but plaques arise when the protein misfolds and begins to accumulate in the brain by ...
These involve labelled proteins targeted at biomarkers, nucleic acid sequences present within cells that are found when the ... The isolate is then exposed to fluoresent dye, which will be luminescent when viewed. Improvements to existing platforms are ...
The use of fluorescent dyes has been explored.[9] These involve labelled proteins targeted at biomarkers, nucleic acid ... The isolate is then exposed to fluoresent dye, which will be luminescent when viewed.[9] ...
... allows one to control the expression of envelope proteins. A frequently used protein is the glycoprotein G (VSV-G ... In cell culture, neutralized pseudoviruses will be prevented from infecting cells and producing the luminescent reporter gene ... Envelope proteins incorporated into the pseudovirus allow the virus to readily enter different cell types with the ... With this method, the foreign viral envelope proteins can be used to alter host tropism or increase or decrease the stability ...
... s are a type of enzyme, made of protein, from bioluminescent organisms. They add to the function of the luciferins ... The term photoprotein was first used to describe the unusual chemistry of the luminescent system of Chaetopterus (a marine ... Polychaete worm). This was meant to distinguish them from other light-producing proteins because these do not exhibit the usual ...
Protein tag Green NM (1975). "Avidin". Advances in Protein Chemistry. 29: 85-133. doi:10.1016/s0065-3233(08)60411-8. PMID ... Ostojic GN, Hersam MC (June 2012). "Biomolecule-directed assembly of self-supported, nanoporous, conductive, and luminescent ... Sinclair JC, Davies KM, Vénien-Bryan C, Noble ME (July 2011). "Generation of protein lattices by fusing proteins with matching ... "Amine Landscaping to Maximize Protein-Dye Fluorescence and Ultrastable Protein-Ligand Interaction". Cell Chemical Biology. 24 ( ...
All these proteins catalyze the oxidation of this substance, an reaction catalogued EC 1.13.12.5. Coelenterazine was ... Both groups unknowingly discovered that the same compound was used in both luminescent systems, however the name of the ... Myctophidae echinoderms such as Amphiura filiformis The compound has also been isolated from organisms that are not luminescent ... simultaneously isolated and characterized by two groups studying the luminescent organisms sea pansy (Renilla reniformis) and ...
The DNA encoding the luminescent protein is incorporated into the laboratory animal either via a viral vector or by creating a ... and green fluorescent protein for continuous imaging of cell culture and animal models". Journal of Biomedical Optics. 16 (4): ...
Branda SS, Chu F, Kearns DB, Losick R, Kolter R (February 2006). "A major protein component of the Bacillus subtilis biofilm ... 23 November 2016). "Salmonella biofilms using luminescent oligothiophenes". NPJ Biofilms and Microbiomes. 2: 16024. doi:10.1038 ... The EPS matrix consists of exopolysaccharides, proteins and nucleic acids. A large proportion of the EPS is more or less ... This means that the genes necessary for the production of proteins that work towards defending the plant against pathogens have ...
Furthermore, the chromatophores contain luminescent protein nanostructures in which tethered pigment granules modify light ... The blood of a cuttlefish is an unusual shade of green-blue, because it uses the copper-containing protein haemocyanin to carry ... "How Egg Case Proteins Can Protect Cuttlefish Offspring?". PLOS ONE. 10 (7): e0132836. Bibcode:2015PLoSO..1032836C. doi:10.1371/ ... oxygen instead of the red, iron-containing protein haemoglobin found in vertebrates' blood. The blood is pumped by three ...
"Luminescent quantum clusters of gold in transferrin family protein, lactoferrin exhibiting FRET" (PDF). Nanoscale. 2 (12): 2769 ... Lactoferrin (larger protein) and a siderophore of an E. Coli cell (smaller protein) are shown. Lactoferrin is a protein found ... protein serine/threonine kinase activator activity. • metal ion binding. • peptidase activity. • protein binding. • serine-type ... The fraction of protein extracted from milk, contains 3.3% RNA,[24] but, the protein preferably binds to double-stranded DNA ...
These include secretory proteins in prokaryotes and eukaryotes and also proteins that are intended to be incorporated in ... Olson and Eglen; Eglen, RM (2007). "beta Galactosidase complementation: A cell-based luminescent assay platform for drug ... were used to detect protein modifications. The technique showed that the antigenic proteins of the non-virulent E.muris is more ... The oxidized protein is then treated with a complex mixture, generating a new conjugate on the membrane. The membrane is then ...
The protein was isolated and mutated to catalyze a bright and sustained luminescent reaction to create an engineered luciferase ... The proteins signal the enzyme for secretion in luminescence, catalyzed by the protein 19 kDa [3,4,7]. The luciferase has many ... with one cysteine in the carboxyl terminus and is distinct from proteins found in other luciferases [4]. The protein is made up ... Protein expression and purification, 56(2), 261-268. Hall, M. P., Unch, J., Binkowski, B. F., Valley, M. P., Butler, B. L., ...
Examples of viral Bcl-2 proteins include the Epstein-Barr virus BHRF1 protein and the adenovirus E1B 19K protein.[104] Some ... Apoptosis is known to be one of the primary mechanisms of targeted cancer therapy.[38] Luminescent iridium complex-peptide ... The adenovirus E1B-55K protein and the hepatitis B virus HBx protein are examples of viral proteins that can perform such a ... these inhibitory proteins target retinoblastoma tumor-suppressing proteins.[83] These tumor-suppressing proteins regulate the ...
C. Zhi, Y. Bando, C. Tang and D. Golberg : «Immobilization of Proteins on Boron Nitride Nanotubes» J. Am. Chem. Soc. 127[49] ( ... X. Li, N. Hanagata, X. Wang, M. Yamaguchi, W. YI, Y. Bando, D. Golberg : «Multimodal luminescent-magnetic boron nitride ... Potential Luminescent and Field-Emitter Nanomaterial» JOURNAL OF PHYSICAL CHEMISTRY C 112[12] (2008) 4735-4742 ... Efficient disentanglement of boron nitride nanotubes using water-soluble polysaccharides for protein immobilization» RSC ...
... usually utilizes an antibody to one or more proteins of interest that are conjugated to enzymes yielding either luminescent or ... Main article: Protein domain. Many proteins are composed of several protein domains, i.e. segments of a protein that fold into ... globular proteins, fibrous proteins, and membrane proteins. Almost all globular proteins are soluble and many are enzymes. ... Protein purification. Main article: Protein purification. To perform in vitro analysis, a protein must be purified away from ...
Prime examples of dishonest signals include the luminescent lure of the anglerfish, which is used to attract prey, or the ... secretes an accessory gland protein during mating that makes them unattractive to other males and thus prevents females from ... The natural world is replete with examples of signals, from the luminescent flashes of light from fireflies, to chemical ...
Pigments, in this case, are minerals which reflect the color green, rather that emitting it through luminescent or ... a protein that carries copper ions in chelation. ...
In the 1960s and 1970s, GFP, along with the separate luminescent protein aequorin (an enzyme that catalyzes the breakdown of ... Wikimedia Commons has media related to Green fluorescent proteins.. *A comprehensive article on fluorescent proteins at ... The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green ... There are many GFP-like proteins that, despite being in the same protein family as GFP, are not directly derived from Aequorea ...
identical protein binding. • TRAIL binding. Cellular component. • integral component of membrane. • membrane. • integral ... Luminescent iridium complex-peptide hybrids, which mimic TRAIL, have recently been synthesized in vitro. These artificial TRAIL ... In the field of cell biology, TNF-related apoptosis-inducing ligand (TRAIL), is a protein functioning as a ligand that induces ... GO:0001948 protein binding. • tumor necrosis factor receptor superfamily binding. • tumor necrosis factor receptor binding. • ...
The action spectra of chlorophyll molecules are slightly modified in vivo depending on specific pigment-protein interactions. ... Luminescent. *Fluorescent *Fluorescent lamp (compact). *Fluorescent induction. *Photoluminescent *laser lamp. *Chemiluminescent ...
Sankaran, R. M.; Holunga, D.; Flagan, R. C.; Giapis, K. P. (2005). "Synthesis of blue luminescent Si nanoparticles using ... yang menyebabkan komponen sel rusak seperti protein, lipid, dan DNA.[31] beberpa penelitian telah menunjukan bahwa penambahan ... Mangolini, L.; Thimsen, E.; Kortshagen, U. (2005). "High-yield plasma synthesis of luminescent silicon nanocrystals". Nano ...
Biologists use it in cellular assays to detect copper, iron, cyanides, as well as specific proteins via western blotting.[2] ... dried bloodstains may thus be made luminescent repeatedly. ...
"Interactions among three distinct CesA proteins essential for cellulose synthesis". 》Proceedings of the National Academy of ... "Nondestructive, real-time determination and visualization of cellulose, hemicellulose and lignin by luminescent ...
Other proteinsEdit. Zinc serves a purely structural role in zinc fingers, twists and clusters.[186] Zinc fingers form parts of ... 2) is used in a number of organic syntheses.[129] Zinc sulfide (ZnS) is used in luminescent pigments such as on the hands of ... In proteins, zinc ions are often coordinated to the amino acid side chains of aspartic acid, glutamic acid, cysteine and ... Wapnir, Raul A. (1990). Protein Nutrition and Mineral Absorption. Boca Raton, Florida: CRC Press. ISBN 0-8493-5227-4.. ...
Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production. ... Desjardin DE, Perry BA, Lodge DJ, Stevani CV, Nagasawa E. Luminescent Mycena: new and noteworthy species. Mycologia. 2010, 102 ... Hydrophobins: the protein-amphiphiles of filamentous fungi. FEMS Microbiology Reviews. November 2005, 29 (5): 877-96. PMID ... Animals and fungi are each other's closest relatives: congruent evidence from multiple proteins. Proceedings of the National ...
Polyphenolic proteins, Polyphenols Raspberry ellagitannin, Tannic acid Not in this Harborne classification are the C6-C7-C6 ... Detection can be made by recombinant luminescent bacterial sensors.[33] Profiling[edit]. Phenolic profiling can be achieved ... The hardening of the protein component of insect cuticle has been shown to be due to the tanning action of an agent produced by ... In plants, VirA is a protein histidine kinase which senses certain sugars and phenolic compounds. These compounds are typically ...
protein kinaze A).[36] Simbioza med lignjem Euprymna scolopes in luminiscenčnimi bakterijami predstavlja ključni ... 2009). "Deep-sea, swimming worms with luminescent "bombs"". Science 325 (5943): 964. doi:10.1126/science.1172488.. ... Specifičen mehanizem je prisoten v dinoflagelatih rodu Gonyaulax, kjer je luciferin vezan na protein, imenovan luciferin ... Pri nekaterih sistemih je prisoten t. i. energijsko prenašalni protein z vezanim fluorescenčnim kromoforom, ki je udeležen v ...
P0DN86[21-165]; Two specific hCGb proteins that differ by three amino acids in positions 2,4 and 117 have been described: type ... which uses antibodies to hCG labeled with an enzyme or a conventional or luminescent dye. Pregnancy urine dipstick tests are ... See also: Receptor/signaling modulators • Signaling peptide/protein receptor modulators • GnRH and gonadotropins ... 1hcn: STRUCTURE OF HUMAN CHORIONIC GONADOTROPIN AT 2.6 ANGSTROMS RESOLUTION FROM MAD ANALYSIS OF THE SELENOMETHIONYL PROTEIN ...
ipRGCs release both pituitary adenylyl cyclase-activating protein (PACAP) and glutamate onto the SCN via a monosynaptic ... rats lacking rods and cones were able to learn to swim toward sequences of vertical bars rather than an equally luminescent ... a light sensitive protein. Therefore they constitute a third class of photoreceptors, in addition to rod and cone cells.[1] ...
Sankaran, R. M.; Holunga, D.; Flagan, R. C.; Giapis, K. P. (2005). "Synthesis of blue luminescent Si nanoparticles using ... proteins, and other biological molecules". Analytical and Bioanalytical Chemistry. 391 (5): 1609-1618. doi:10.1007/s00216-007- ... Mangolini, L.; Thimsen, E.; Kortshagen, U. (2005). "High-yield plasma synthesis of luminescent silicon nanocrystals". Nano ... which in turn can damage cellular components such as proteins, lipids and DNA.[35] Some studies have also demonstrated that ...
Fin skeletons are elongated and supported with soft and unsegmented rays named ceratotrichia, filaments of elastic protein ... and megamouth sharks make suction feeding more efficient by using the luminescent tissue inside of their mouths to attract prey ...
In place of histones, dinoflagellate nuclei contain a novel, dominant family of nuclear proteins that appear to be of viral ... Dinoflagellate bioluminescence is controlled by a circadian clock and only occurs at night.[72] Luminescent and nonluminescent ... "Loss of nucleosomal DNA condensation coincides with appearance of a novel nuclear protein in dinoflagellates". Current Biology ...
Wiedenmann, Jörg (2008). "Marine proteins". In Patrick J. Walsh (ed.). Oceans and human health: risks and remedies from the ... use of the term cypridinid solves the Cypridina/Vargula dilemma for naming the constituents of the luminescent system of ...
Some of the most common assays are: ELISAs Protein and cell growth assays Protein:protein interactions Reporter assays Nucleic ... Some plate readers offer filter wheel or tunable wavelength monochromator optical systems for selecting specific luminescent ... For example, large molecules (e.g. proteins) in solution, which rotate relatively slowly because of their size, will emit ... protein and nucleic acid quantification or enzyme activity assays (i.e. in the MTT assay for cell viability). A light source ...
He established that, similarly to many proteins and other biomolecules, nanoparticles can self-organize into chains, sheets, ... "Spontaneous Organization of Single CdTe Nanoparticles into Luminescent Nanowires". Science. 297 (5579): 237-40. Bibcode:2002Sci ...
Nordgren, I. K.; Tavassoli, A. (2014). "A bidirectional fluorescent two-hybrid system for monitoring protein-protein ... A cloud of luminescent material is expelled, distracting or repelling a potential predator, while the animal escapes to safety ... It produces greenish luminescent mucus which may have an anti-predator function. The marine snail Hinea brasiliana uses flashes ... Pyrosomes are colonial tunicates and each zooid has a pair of luminescent organs on either side of the inlet siphon. When ...
... lipid anchored protein - lipid bilayer - lipoprotein - Liquid - List of compounds - list of gene families - locus - luminescent ... protein - protein biosynthesis - Protein Data Bank - protein design - protein expression - protein folding - protein isoform - ... protein P16 - protein P34cdc2 - protein precursor - protein structure prediction - protein subunit - protein synthesis - ... proto-oncogene protein C-kit - proto-oncogene proteins c-abl - proto-oncogene proteins c-bcl-2 - Proto-oncogene proteins c-fos ...
... polypeptides and proteins in gels and on solid supports, using neutral or anionic complexes of transition metals. ... Proteins appear as red to orange luminescent bands on a clear background. Compared to Example 7, this is the preferred method ... Luminescent Protein Staining. US20090131640 *. Nov 20, 2008. May 21, 2009. Bio-Rad Laboratories, Inc.. Photoluminescent metal ... Spotted proteins appear as red to orange luminescent bands on a faint pink to faint blue background. By comparison, staining ...
Green Fluorescent Protein as a Noninvasive Stress Probe in Resting Escherichia coli Cells Hyung Joon Cha, Ranjan Srivastava, ... Green Fluorescent Protein as a Reporter To Monitor Gene Expression and Food Colonization by Aspergillus flavus Wanglei Du, ... Dual Labeling with Green Fluorescent Proteins for Confocal Microscopy Stacie E. Cowan, Eric Gilbert, Artem Khlebnikov, J. D. ... Use of Green Fluorescent Protein To Tag Lactic Acid Bacterium Strains under Development as Live Vaccine Vectors Marie-Claude ...
... bioluminescent pull-down workflow for validating protein:protein interaction results obtained with NanoBRET™ assays. ... NanoBRET™ Protein:Protein Interaction Assay. To perform this study, we used two well-characterized protein interactions: p53: ... protein and NanoLuc® luciferase as protein fusion tags. (1) (2) NanoLuc® luciferase is a small (19kDa) luminescent reporter ... A Luminescent Pull-Down Approach to Confirm NanoBRET™ Protein Interaction Assays. Leta Steffen and Jacqui Méndez-Johnson. ...
Packaging luminescent proteins in the form of rubber, a white-producing LED eliminates the need for expensive inorganic ... Their technique involves introducing luminescent proteins into a polymer matrix to produce the luminescent rubber. The team ... 13, 2016 - Packaging luminescent proteins in the form of rubber, a white-producing LED eliminates the need for expensive ... The proteins have] luminescent properties that remain intact during the months of storage under different environmental ...
Conjugation of luminescent quantum dots with antibodies using an engineered adaptor protein to provide new reagents for ... The conjugation strategy employs an engineered molecular adaptor protein, attached to the QDs via electrostatic/hydrophobic ... QD-antibody conjugates were successfully used in fluoroimmunoassays for detection of both a protein toxin (staphylococcal ... We describe the preparation and characterization of bioinorganic conjugates made with highly luminescent semiconductor CdSe-ZnS ...
Universal, homogenous, and high throughput luminescent assay technologies to monitor protein kinase activity. Said Goueli, ... Universal, homogenous, and high throughput luminescent assay technologies to monitor protein kinase activity ... Universal, homogenous, and high throughput luminescent assay technologies to monitor protein kinase activity ... Universal, homogenous, and high throughput luminescent assay technologies to monitor protein kinase activity ...
Luminescent solar concentrators (LSCs) are a solution to overcome the mismatch between solar cell absorption and the solar ... Environmentally friendly luminescent solar concentrators based on an optically efficient and stable green fluorescent protein† ... Environmentally friendly luminescent solar concentrators based on an optically efficient and stable green fluorescent protein C ... Luminescent solar concentrators (LSCs) are a solution to overcome the mismatch between solar cell absorption and the solar ...
Bioluminescent Proteins; Photoproteins. On-line free medical diagnosis assistant. Ranked list of possible diseases from either ... Luminescent Proteins (Bioluminescent Proteins; Photoproteins). proteins which are involved in the phenomenon of light emission ...
... Vorotnikov, Yuri A.; Pozmogova, Tatiana N.; Solovieva, Anastasiya O ... The SMPs were modified to allow their functionalisation by a protein, which then delivered the protein (GFP) efficiently into ... Luminescent silica mesoparticles for protein transduction. Materials Science and Engineering: C, 96, 530-538. doi:10.1016/j. ... the luminescent SMPs offer a cheap and trackable alternative to existing materials for cellular internalisation of proteins, ...
Luminescent silica mesoparticles for protein transduction. Yuri A. Vorotnikov, Tatiana N. Pozmogova, Anastasiya O. Solovieva, ... Luminescent silica mesoparticles for protein transduction. / Vorotnikov, Yuri A.; Pozmogova, Tatiana N.; Solovieva, Anastasiya ... title = "Luminescent silica mesoparticles for protein transduction",. abstract = "Unlike silica nanoparticles, the potential of ... Fingerprint Dive into the research topics of Luminescent silica mesoparticles for protein transduction. Together they form a ...
The fluorescent proteins, luciferases, and a simplified protocol for protein purification were then shared with a local ... B. Fluorescent protein purification. Fluorescent proteins were isolated to quantify and measure fluorescence. The protocol to ... B) Graph shows amount of fluorescent protein (ug) present in 1mL of culture for each of the fluorescent proteins. The ratio ... and proteins were analyzed by adding reagent to dilutions of the purified proteins. Absorbance was measured using a TECAN M1000 ...
This method is highly amenable to the detection of G protein-coupled receptor (GPCR) interactions with proteins critical for ... This method is highly amenable to the detection of G protein-coupled receptor (GPCR) interactions with proteins critical for ... The BRET method utilizes heterologous co-expression of fusion proteins linking one protein of interest (GPCR) to a ... The BRET method utilizes heterologous co-expression of fusion proteins linking one protein of interest (GPCR) to a ...
A bioluminescent antibody-free method to detect HiBiT-tagged proteins blotted on nitrocellulose membranes in less than 10 ... Fast Luminescent Detection of Proteins on Blots *Determine protein size and quantify expression on blots ... A luminescent live-cell method to detect HiBiT-tagged proteins on the cell surface or secreted into the medium. ... Learn how the tiny 11-amino-acid luminescent tag called HiBiT can quantify protein abundance even at endogenous levels. CRISPR- ...
Purchase Fluorescent and Luminescent Probes for Biological Activity - 2nd Edition. Print Book & E-Book. ISBN 9780124478367, ... The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as ... Fluorescent and Luminescent Probes for Biological Activity 2nd Edition. A Practical Guide to Technology for Quantitative Real- ... S.R. Kain, Enhanced Variants of the Green Fluorescent Protein for Greater Sensitivity, Different Colors, and Detection of ...
Luminescent Proteins. Grant support. *R01 AI064668/AI/NIAID NIH HHS/United States ... Imaging multiple fluorescent proteins (FPs) by two-photon microscopy has numerous applications for studying biological ... Fluorescent protein spectra and selective excitations. (a) Two-photon brightness (dotted curves) and fluorescence emission ... Two-photon imaging of multiple fluorescent proteins by phase-shaping and linear unmixing with a single broadband laser.. ...
Structure and formation of highly luminescent protein-stabilized gold clusters D. M. Chevrier, V. D. Thanthirige, Z. Luo, S. ... Findings on the structure and formation of luminescent protein-stabilized gold clusters reveal interlocked gold-thiolate rings ... We report a luminescent micelle that is prepared through the self-assembly of an amphiphilic, neutral Pt(II) complex with ... Examination of the luminescent properties of these aggregates - narrow red-shifted absorption and emission bands, minimal ...
Highly luminescent carbon dots (C-dots) were synthesized by the one-pot simple hydrothermal method directly from lemon juice ... Lemon contains very little fat and protein. Its composition consists mainly of carbohydrates (10%) and water (88-89%). ... Green Synthesis of Highly Luminescent Carbon Quantum Dots from Lemon Juice. Bui Thi Hoan. ,1. ,. 2 Phuong Dinh Tam. ,1. ,. 3 ... Luminescent spectra of the C-dots at 280°C in different hydrothermal times of 3, 6, 9, and 12 h are shown in Figure 8(a). It is ...
It was established that the luminescent label retains unchanged its emission characteristics not only in the polypeptides but ... The growing attention to the luminescent nanocarriers is strongly stimulated by their potential application as drug delivery ... in situ generation of intrinsically fluorescent recognition ligands for protein detection. Chem. Comm. 47, 2294-2296 (2011). ... Investigation of the luminescent properties of 2-P(Z)Lys and 2-P(Bzl)Glu polypeptides in organic medium (CHCl3/DMSO) revealed ...
Classification: LUMINESCENT PROTEIN. *Organism(s): Aequorea victoria. *Expression System: Escherichia coli BL21(DE3) ... supercharged protein assembly (SuPrA). We show that supercharged variants of green fluorescent protein can assemble into a ... Symmetrical protein oligomers are ubiquitous in biological systems and perform key structural and regulatory functions. However ... Symmetrical protein oligomers are ubiquitous in biological systems and perform key structural and regulatory functions. However ...
Classification: LUMINESCENT PROTEIN. *Organism(s): Aequorea victoria. *Expression System: Escherichia coli. *Mutation(s): Yes ... Green Fluorescent Protein. A. 236. Aequorea victoria. Mutation(s): 2 Gene Names: GFP. ... The reactivity described here further expands the chemical diversity observed in the active site of GFP-like proteins, and may ... Y66L Variant of Enhanced Green Fluorescent Protein with 374-nm Absorbing Chromophore. *DOI: 10.2210/pdb1Z1Q/pdb ...
Helminth Proteins / genetics* * Helminth Proteins / physiology* * Luminescent Proteins / genetics * Luminescent Proteins / ... Here we show that CLK-1 is fully active when fused to green fluorescent protein and is found in the mitochondria of all somatic ...
In sink leaves, biolistic bombardment of plasmids encoding GFP-fusion proteins demonstrated that proteins with an Mr up to 50 ... Green Fluorescent Proteins * Luminescent Proteins / genetics * Luminescent Proteins / metabolism * Plant Leaves / metabolism ... In sink leaves, biolistic bombardment of plasmids encoding GFP-fusion proteins demonstrated that proteins with an Mr up to 50 ... Simple, but not branched, plasmodesmata allow the nonspecific trafficking of proteins in developing tobacco leaves Cell. 1999 ...
Zinc Finger Protein Inhibitors. CXCR4 Inhibitors. Xanthine Oxidase Inhibitors. Miscellaneous Protein Inhibitors and Conclusions ... Transition-Metal Containing Luminescent Agents. Lanthanide-Based Luminophores. Nanoparticle-Based Luninophores. Conclusions and ... Protein Phosphatase Inhibitors. Trypsin and Thrombin Inhibitors. Cysteine Protease Inhibitors and Glutathione Transferase ...
RT @_atanas_: Self-luminescent photodynamic therapy using breast cancer targeted proteins #luminescent #photodynamictherapy #b… ... RT @_atanas_: Self-luminescent photodynamic therapy using breast cancer targeted proteins #luminescent #photodynamictherapy # ... DeepMind AI cracks 50-year-old problem of protein folding #AI #ProteinFolding More from #DHPSP, INPST, and CRBIOTECH: https:// ... DeepMind AI cracks 50-year-old problem of protein folding #AI #ProteinFolding More from #DHPSP, INPST, and CRBIOTECH: https:// ...
Lanthanide Ions of Electron Transfer in Proteins. In Metal Ions in Biological Systems; Sigel, A., Sigel, H., Eds.; Marcel ... Luminescent Sensor Based on Ln(III) Ternary Complexes for NAD(P)H Detection by Filip Smrčka ... The luminescent ternary Eu(III) complex is also electroactive due to possibility of the reduction pathway Eu(III) → Eu(II) ... Bűnzli, J.C. On the design of highly luminescent lanthanide complexes. Coord. Chem. Rev. 2015, 293-294, 19-47. [Google Scholar ...
... required for luminescent XpressPack™ from CHEMICON,The XpressPack Luminescent Detection System is intended for use in the ... The XpressPack Luminescent Detection System is a hybridizatio,biological,biology supply,biology supplies,biology product ... Protein DetectorTM Microarray Dot Blot Kits overcome ... and quantitation over a wide range in ... ) detection. They are ideal ... Luciferase Luminescent Assay Kit, LucLite from PerkinElmer. 3. The LAS-1000 plus Luminescent Image Analysis System from ...
Protein (lb); protein (nb); Protéin (su); Protein (hif); 朊 (lzh); بروتين (ar); Protein (br); ပရိုတိန်း (my); 蛋白質 (yue); Белок ( ... प्रोटिन (dty); Prótín (is); Protein (ms); protein (tr); لحمیات (ur); Bielkovina (sk); білок (uk); 蛋白质 (zh-cn); Protein (gsw); ... protein (sco); Уураг (mn); protein (nn); ಪ್ರೋಟೀನ್ (kn); پرۆتین (ckb); protein (en); fehérje (hu); પ્રોટિન (gu); प्रोटिन (new); ... protein (hr); протеин, белки, протеины (ru); протеин (tt-cyrl); protein, Bílkoviny ve výživě člověka (cs); Protein (nutrien) ( ...
Self-assembled luminescent CdSe-ZnS quantum dot bioconjugates prepared using engineered poly-histidine terminated proteins. ... Self-assembled luminescent CdSe-ZnS quantum dot bioconjugates prepared using engineered poly-histidine terminated proteins. / ... Self-assembled luminescent CdSe-ZnS quantum dot bioconjugates prepared using engineered poly-histidine terminated proteins. In ... These results open up the possibility to conjugate luminescent QDs to a whole range of proteins to form QD bioconjugates that ...
It has been predicted that there are several hundred thousand pairs of protein-protein int... ... Luminescent detection of Protein-Protein Interaction by tagging tiny peptides - Department of Life Science and Technology, ... As protein-protein interactions play major roles for life phenomenon, understanding of protein-protein interaction is very ... In protein-protein interaction assay fluorescent proteins and luciferase enzymes often fused to interacting pair. However, the ...
The proteins use a Bio-LED, featuring white light, with colored luminescent proteins. This technology can effectively be used ... luminescent proteins. The proteins can be used in color filters and backlighting systems, and are made with 3D printing, ... Tagged with: 3d printing research • FAU • germany • journal article • LCD screens • LED • LED lighting • luminescent proteins ... The details of the second display component enhanced by the luminescent proteins, the color filter, are described in an paper ...
  • The enhanced green fluorescent protein (eGFP) was efficiently applied as an optically active center. (rsc.org)
  • These results are higher than the figures of merit for naturally-based fluorescent proteins in aqueous media, with the advantage of presenting enhanced photostability when stored under ambient conditions. (rsc.org)
  • GPCR) to a bioluminescent donor enzyme, a variant of Renilla luciferase (luminophore: Rluc, hRluc, Rluc2, or Rluc8), and a second protein of interest (e.g., β-arrestin) to an acceptor fluorophore, a variant of green fluorescent protein (Venus or GFP10). (frontiersin.org)
  • Fluorescent proteins including BFP, GFP, YFP, OFP and RFP were purified and evaluated in an effort to standardize measurements that are useful for modeling and sharing this data across the synthetic biology community. (plos.org)
  • The fluorescent proteins, luciferases, and a simplified protocol for protein purification were then shared with a local community lab. (plos.org)
  • Two-photon imaging of multiple fluorescent proteins by phase-shaping and linear unmixing with a single broadband laser. (nih.gov)
  • Imaging multiple fluorescent proteins (FPs) by two-photon microscopy has numerous applications for studying biological processes in thick and live samples. (nih.gov)
  • Fluorescent protein spectra and selective excitations. (nih.gov)
  • Images of two-photon excitation of a mixed sample, in which each mammalian COS-7 cell only expresses one of either mAmetrine, TagRFPt or mKate2 fluorescent proteins. (nih.gov)
  • V.L. Singer and R.P. Haugland , Fluorescent Imaging of Nucleic Acids and Protein in Gels. (elsevier.com)
  • S.R. Kain , Enhanced Variants of the Green Fluorescent Protein for Greater Sensitivity, Different Colors, and Detection of Apoptosis. (elsevier.com)
  • We show that supercharged variants of green fluorescent protein can assemble into a variety of architectures including a well-defined symmetrical 16-mer structure that we solved using cryo-electron microscopy at 3.47 Å resolution. (rcsb.org)
  • The mechanism of chromophore biosynthesis in green fluorescent protein (GFP) is triggered by a spontaneous main chain cyclization reaction of residues 65-67. (rcsb.org)
  • Here we show that CLK-1 is fully active when fused to green fluorescent protein and is found in the mitochondria of all somatic cells. (nih.gov)
  • In protein-protein interaction assay fluorescent proteins and luciferase enzymes often fused to interacting pair. (titech.ac.jp)
  • However, the rather large sizes of the fluorescent proteins and the luciferase enzymes sometimes cause the problems of the misfolding and the steric hindrance. (titech.ac.jp)
  • In a frequently used intracellular protein-protein interaction assay Protein-fragment Complementation Assay (PCA), a sensor enzyme or a fluorescent protein is divided into two fragments. (titech.ac.jp)
  • Presently using a recombinant virus containing the gene for green fluorescent protein we will attempt to localize the cellular reservoir latent virus by confocal microscopy. (duke.edu)
  • This physical property makes conjugated polymers an indispensible tool in the toolbox of fluorescent reporters used for distinguishing protein aggregates. (diva-portal.org)
  • Learn the fascinating uses and history of the green fluorescent protein (GFP). (exploratorium.edu)
  • In Renilla reniformis, RLuc is found in membrane-bound intracellular structures within specialized light emitting cells, and is coupled with a closely interacting green fluorescent protein (RrGFP), and a Ca++ activated luciferin binding protein (RrLBP). (wikipedia.org)
  • A C -terminal green fluorescent protein (GFP) fusion to AgrC served as a reporter for monitoring protein expression levels in real time. (mdpi.com)
  • To understand better the dynamics of intermediate filament formation, transport, and stability in a healthy, living cell , we inserted neurofilament heavy chain (NFH)-green fluorescent protein (GFP) fusion constructs in adenoviral vectors with tetracycline (tet)-regulated promoters. (nasa.gov)
  • Luminescent Eel Muscles Fluorescent Protein Revolution into Clinic. (wikipedia.org)
  • According to W.O. Gordon et al, the small size of luminescent inorganic nanoparticles allows rare-earth ions to replace fluorescent molecules or complexes in analytical applications. (photonics.com)
  • RXR, PPAR, and the coactivator SRC-1 proteins will be tagged with luminescent or fluorescent proteins. (epa.gov)
  • Aequorea , the jellyfish from which green fluorescent protein is derived. (princeton.edu)
  • Shimomura, who was a researcher in the Department of Biology from 1960 to 1982, will be honored along with two other researchers for his work on the green fluorescent protein (GFP) in jellyfish. (princeton.edu)
  • Gary Borisy, director and CEO of Woods Hole, agreed that Shimomura's work on fluorescent proteins has proven crucial for generations of scientists. (princeton.edu)
  • They also isolated a second protein that was slightly greenish in sunlight, yellowish in the light from a light bulb and fluorescent green in ultraviolet light. (princeton.edu)
  • They called it the green protein, but it eventually came to be called the green fluorescent protein. (princeton.edu)
  • 9. The method of claim 8 , wherein the fluorescent molecule is green fluorescent protein, lumazine, or yellow fluorescent protein. (google.co.uk)
  • They developed fluorescent protein from jellyfish for use in tracing cell molecules. (latimes.com)
  • A UC San Diego pharmacologist and two other U.S.-based scientists won the 2008 Nobel Prize in Chemistry on Wednesday for their development of a green fluorescent protein from jellyfish that has provided researchers their first new window into the workings of the cell since the development of the microscope. (latimes.com)
  • The story of the fluorescent protein starts with Shimomura. (latimes.com)
  • From this material, they isolated a blue luminescent protein called aequorin and a green fluorescent protein, commonly called GFP. (latimes.com)
  • QD-antibody conjugates were successfully used in fluoroimmunoassays for detection of both a protein toxin (staphylococcal enterotoxin B) and a small molecule (2,4,6-trinitrotoluene). (nih.gov)
  • This method is highly amenable to the detection of G protein-coupled receptor (GPCR) interactions with proteins critical for regulating their function, such as β-arrestins. (frontiersin.org)
  • Luminescent detection of Protein-Protein Interaction b. (titech.ac.jp)
  • The XpressPack Luminescent Detection System is intended for use in the quantitative detection of amplified DNA generated by an in-house validated method for in vitro nucleic acid amplification of a selected target of interest. (bio-medicine.org)
  • The XpressPack Luminescent Detection System is a hybridization capture assay designed to detect amplified nucleic acid specific to the target of interest. (bio-medicine.org)
  • Typically, expression levels of experimentally introduced proteins are not regulated, and high levels are often preferred to facilitate detection. (nasa.gov)
  • A luminescent ruthenium complex for ultrasensitive detection of proteins immobilized on membrane supports. (thermofisher.com)
  • Kumagai T, Abe K, Yoshida W, Ikebukuro K (2015) DNA Detection Technology Using Zinc Finger Protein. (omicsonline.org)
  • Using zinc finger protein for DNA detection element, simple, accurate and sensitive DNA detection can be achieved. (omicsonline.org)
  • In this review, dsDNA detection using zinc finger protein is described and compared with recent advanced technology. (omicsonline.org)
  • Following extensive washing, a mixture of detection antibodies is added to detect the captured target proteins. (cellsignal.com)
  • The invention is directed to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system. (google.ca)
  • Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system. (google.ca)
  • The invention also comprises proteins prepared using misaminoacylated tRNAs which can be utilized in pharmaceutical compositions for the treatment of diseases and disorders in humans and other mammals, and kits which may be used for the detection of diseases and disorders. (google.ca)
  • Subsequent time resolved detection of the metal-centered luminescent probe yields the desired signal. (wikipedia.org)
  • We successfully achieved three indispensable basic elements for the system as follows: (1) construction of novel protein-expression control system, (2) construction of detection system for self-assembly reaction as a first step in establishing the analysis system of protein-protein interaction inside cells, (3) construction of cell array system for high-throughput analysis. (nii.ac.jp)
  • Rubber with red, green and blue luminescent proteins used to produce BioLEDs. (photonics.com)
  • It took the team months to purify just a few drops of the blue luminescent material from the liquid. (princeton.edu)
  • The invention relates to the staining of poly(amino acids), including peptides, polypeptides and proteins in gels and on solid supports, using neutral or anionic complexes of transition metals. (google.com)
  • It is applicable to all kinds of kinase substrates regardless of their nature with no prior modification (peptides, protein, polymer, lipids, and sugars). (aacrjournals.org)
  • The assay is robust as indicated by the high Z' values (over 0.8), homogenous, can be completed in one step after completion of kinase reaction, does not require antibodies or custom synthesized substrates, and is ideal to search for optimal substrates in a collection of peptides, proteins, lipids in one assay format and to screen for ATP and Non-ATP competitive inhibitors. (aacrjournals.org)
  • γ-Secretase inhibitors (GSIs) reduce amyloid-β (Aβ) peptides but inevitably increase the β-C-terminal fragment (β-CTF) of amyloid precursor protein (APP), potentially having undesirable effects on synapses. (jneurosci.org)
  • Reactive oxygen species are increased by environmental factors and intracellular metabolism, which cause the accumulation of abnormally high levels of damaged proteins in cells 11 , 12 . (nature.com)
  • Most importantly, expression of adipose FA binding protein (A-FABP) in macrophages facilitated metabolism of excess sFAs for Cer synthesis. (jimmunol.org)
  • The BRET method utilizes heterologous co-expression of fusion proteins linking one protein of interest (GPCR) to a bioluminescent donor enzyme, a variant of Renilla luciferase, and a second protein of interest (β-arrestin) to an acceptor fluorophore. (frontiersin.org)
  • The BRET method is based on heterologous co-expression of fusion proteins linking one protein of interest (e.g. (frontiersin.org)
  • In sink leaves, biolistic bombardment of plasmids encoding GFP-fusion proteins demonstrated that proteins with an Mr up to 50 kDa could move freely through plasmodesmata. (nih.gov)
  • This system is suitable for cloning of the genes and for directing the synthesis of large amounts of the fusion proteins αB-Cry/A-chain (αB-AC) and αB-Cry/B-chain (αB-BC). (jascoinc.com)
  • The construction of vectors, their efficient expression in Escherichia coliand simple purification of the fusion proteins and two insulin chains are described. (jascoinc.com)
  • A large amount of the recombinant fusion proteins with high purity was obtained by applying a single step anion exchange chromatography or metal chelate affinity. (jascoinc.com)
  • The insulin A- and B-chain were released from the fusion proteins using cyanogen bromide cleavage. (jascoinc.com)
  • In one embodiment, the invention comprises the use of non-cleavable multimerized ubiquitin fusion proteins to destabilize a target protein, such as a reporter moiety. (freepatentsonline.com)
  • Conjugation of luminescent quantum dots with antibodies using an engineered adaptor protein to provide new reagents for fluoroimmunoassays. (nih.gov)
  • We describe the preparation and characterization of bioinorganic conjugates made with highly luminescent semiconductor CdSe-ZnS core-shell quantum dots (QDs) and antibodies for use in fluoroimmunoassays. (nih.gov)
  • The conjugation strategy employs an engineered molecular adaptor protein, attached to the QDs via electrostatic/hydrophobic self-assembly, to link the inorganic fluorophore with antibodies. (nih.gov)
  • To further assess the state of viral activity in latent infection we have tested the activity of monoclonal antibodies against putative latent viral proteins. (duke.edu)
  • Abcam: antibodies, proteins, kits. (abcam.com)
  • After incubation with cell lysates, the spotted antibodies capture the target proteins. (cellsignal.com)
  • A general method for the rapid characterization of tyrosine-phosphorylated proteins by mini two-dimensional gel electrophoresis. (thermofisher.com)
  • There has been a great deal of interest in the fabrication and characterization of highly luminescent nanoparticles because of their potential applications as nanosensors and as biosensors for applications such as molecular diagnostics. (photonics.com)
  • The present invention relates to the discovery, identification, and characterization of novel human polynucleotides encoding proteins that share sequence similarity with animal kinases. (google.es)
  • The bioluminescence resonance energy transfer (BRET) technique has become extremely valuable for the real-time monitoring of protein-protein interactions in live cells. (frontiersin.org)
  • Therefore, the enhanced BRET methodology has not only enabled live cell compound screening as we have recently published, it now provides a new level of sensitivity for monitoring specific transient, weak or hardly detectable protein-protein complexes, including agonist-independent GPCR/β-arrestin interactions. (frontiersin.org)
  • The 16-mer is composed of two stacked rings of octamers, in which the octamers contain supercharged proteins of alternating charges, and interactions within and between the rings are mediated by a variety of specific electrostatic contacts. (rcsb.org)
  • The ready assembly of this structure suggests that combining oppositely supercharged pairs of protein variants may provide broad opportunities for generating novel architectures via otherwise unprogrammed interactions. (rcsb.org)
  • It has been predicted that there are several hundred thousand pairs of protein-protein interactions in one cells of human. (titech.ac.jp)
  • As protein-protein interactions play major roles for life phenomenon, understanding of protein-protein interaction is very important for various fields such as basic biology, diagnosis and drug discovery. (titech.ac.jp)
  • The interactions of proteins having complex structures were often undetected by conventional assays due to the probe size. (titech.ac.jp)
  • Now we are improving this technology to develop probes that can detect many more protein-protein interactions. (titech.ac.jp)
  • NanoLuc complementation reporter optimized for accurate measurement of protein interactions in cells. (titech.ac.jp)
  • Knowledge of protein-protein interactions and their binding sites is indispensable for in-depth understanding of the networks in living cells. (mdpi.com)
  • Most proteins whose functions are essential to life are associated with protein-protein interactions [ 1 ]. (mdpi.com)
  • Protein interactions will be monitored in cells, in real-time, using bioluminescence resonance energy transfer. (epa.gov)
  • Bioimaging based on luminescent microscopy represents one of the most powerful analytical techniques in the life sciences because of its high sensitivity accompanied with simplicity and low cost. (nature.com)
  • NUREMBERG, Germany, Jan. 13, 2016 - Packaging luminescent proteins in the form of rubber, a white-producing LED eliminates the need for expensive inorganic materials. (photonics.com)
  • Highly luminescent carbon dots (C-dots) were synthesized by the one-pot simple hydrothermal method directly from lemon juice using different temperatures, time, aging of precursors, and diluted solvents to control the luminescence of C‐dots. (hindawi.com)
  • Carbon quantum dots (C-dots) have received considerable attention in bioimaging because of their highly biocompatibility, luminescent properties, and sphere-shaped nanoparticles [ 1 , 2 ]. (hindawi.com)
  • It was established that the luminescent label retains unchanged its emission characteristics not only in the polypeptides but also in more complicated nanoaggregates such as the polymer derived amphiphilic block-copolymers and self-assembled nanoparticles. (nature.com)
  • Another way of monitoring of drug-carrier localization is preparation of nanoparticles containing covalently bound dye molecules, which represent a stable form of luminescent nanoobjects. (nature.com)
  • 2 A rapid and inexpensive method of preparing luminescent nanoparticles of europium oxide has been demonstrated recently by using a microwave-assisted surface chemistry. (photonics.com)
  • These data suggest that the Y219A mutation generates a G protein-biased state primarily by conformational selection against GRK coupling, rather than against β-arrestin. (sciencemag.org)
  • 40. The method of claim 39, wherein said ubiquitin homolog comprises a mutation that prevents cleavage by α-NH-ubiquitin protein endoproteases. (freepatentsonline.com)
  • Energy transfer implies that these molecules are in close proximity, and therefore, the proteins of interest (e.g. (frontiersin.org)
  • 1) Y Ohmuro-Matsuyama & H Ueda, Homogeneous noncompetitive luminescent immunodetection of small molecules by ternary protein fragment complementation. (titech.ac.jp)
  • This enables the filtering of their signal from the in vivo background originating from organic molecules with luminescent decay on the ns time scale [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 ]. (mdpi.com)
  • Biologists for years have been trying to visualize the movement and action of proteins and other molecules in and around cells and tissues. (nsti.org)
  • tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives, or substances recognized by the protein synthesizing machinery. (google.ca)
  • The LuxI-like proteins are autoinducer synthases and are responsible for production of acyl homoserine lactone autoinducer signal molecules, and the LuxR-like proteins are transcriptional activators whose activity is regulated by interaction with a cognate acyl homoserine lactone autoinducer ( 16 , 20 ). (asm.org)
  • The protein can be attached to any of the 10,000 individual molecules within a living cell, allowing researchers for the first time to trace their paths as they wind through the complex pathways of life. (latimes.com)
  • We have further established the gene map location encoding one of these unique proteins and is found to be distinct from the immediate early genes. (duke.edu)
  • These products are often proteins, but in non-protein coding genes such as ribosomal RNA (rRNA) genes or transfer RNA (tRNA) genes, the product is a functional RNA. (wikibooks.org)
  • Genes are expressed by being transcribed into RNA, and this transcript may then be translated into protein. (wikibooks.org)
  • We report a simple and versatile approach for the conjugation of luminescent CdSe-ZnS core-shell quantum dots (QDs) to proteins through coordination of engineered C-terminal oligohistidine sequences. (uthscsa.edu)
  • LuxP is similar to the periplasmic ribose binding proteins of Escherichia coli and Salmonella typhimurium . (asm.org)
  • These findings suggest that the degradation of different PIF proteins might be controlled by specific E3 ligase complexes. (elifesciences.org)
  • Consequently, these lanthanide complexes have potential applications in bioassays and luminescent probes ( Hemmila and Webb, 1997 ). (frontiersin.org)
  • It is the special photophysical behavior-the extreme brightness coupled with the switch-like character-that makes the EP2-19G2 antibody attractive as a potential biosensor and as a model for the development of other equally potent types of luminescent antibody-chromophore complexes. (scripps.edu)
  • In 1988, Chalfie heard about GFP and thought it would be useful for tracing the fate of proteins in the roundworm, Caenorhabditis elegans, which is widely used in biological studies because it is transparent, allowing researchers to study its organs under a microscope. (latimes.com)
  • Attention to lanthanide probes increased greatly in the mid-1970s when Finnish researchers proposed Eu(III), Sm(III), Tb(III), and Dy(III) polyaminocarboxylates as luminescent sensors in time-resolved luminescent (TRL) immunoassays. (wikipedia.org)
  • Thus nanometer- and micrometer-size phosphors made of rare-earth doped metal oxides are promising for use as a luminescent tag or reporter for affinity or immunoassays in biomedical, environmental, food quality and drug testing probes. (photonics.com)
  • The bar graph shows measurements of four different target proteins. (cellsignal.com)
  • This allows the researcher to measure four different target proteins from a single well simultaneously. (cellsignal.com)
  • PathScan ® Cell Growth 4-Plex Array Kit detects endogenous levels of all target proteins. (cellsignal.com)
  • In another embodiment the invention provides for a generalized way of coordinately regulating the cellular concentration of a plurality of target proteins. (freepatentsonline.com)
  • In one aspect of this method, the target proteins are operatively coupled to a ubiquitin fusion protein via a linker containing a protease cleavage site. (freepatentsonline.com)
  • The reporter plasmid encodes a reporter protein (RFP or Gaussia luciferase) under control of T7 RNAP. (plos.org)
  • In one aspect of this method the constructs also comprises a linker that operatively couples the reporter moiety to the multimerized ubiquitin fusion protein. (freepatentsonline.com)
  • In this embodiment, enzymatic modification of the linker results in a modulation of the coupling of the reporter protein to the multimerized ubiquitin domains resulting in a change in the stability of the reporter moiety. (freepatentsonline.com)
  • The environment of individual tryptophans in known protein structures and the effectiveness of four commercial robotic UV microscopes to illuminate tryptophan-containing protein crystals by either tryptophan fluorescence (epi-illumination) or absorbance (transmission) are evaluated. (iucr.org)
  • In agreement with other studies, tryptophan residues are found on average to be largely buried in protein structures (with ∼84% of their surface area buried) and to be surrounded by partially polar microenvironments (with ∼43% of their surface area covered by polar residues), which suggests an inherent degree of fluorescence signal quenching. (iucr.org)
  • Transgenic way of attaching green fluorescence proteins started to help in such investigation. (nsti.org)
  • Protein expression levels were analyzed by the microscopic assessment of the whole-cell fluorescence. (mdpi.com)
  • Luciferase proteins from Photinus (firefly), Renilla and Gaussia were also characterized and tested as reporters. (plos.org)
  • We developed an improved split luciferase complementation assay coupled to a powerful genetic system to show that colocalization within the same membrane domain enables formation of RAS dimers/coclusters with itself and other membrane-associated proteins. (pnas.org)
  • 12. The method of claim 11 , wherein the bioluminescent molecule is a luciferase protein. (google.co.uk)
  • Here we examine the potential of luminescent (namely, octahedral molybdenum cluster doped) SMPs synthesised by a simple one-pot reaction for the labelling of cells and for protein transduction into larynx carcinoma (Hep-2) cells using GFP as a model protein. (worktribe.com)
  • The SMPs were modified to allow their functionalisation by a protein, which then delivered the protein (GFP) efficiently into the cells. (worktribe.com)
  • Inoculation of sink leaves with a movement protein-defective virus showed that virally expressed GFP, but not viral RNA, was capable of trafficking between sink cells during infection. (nih.gov)
  • The growing attention to the luminescent nanocarriers is strongly stimulated by their potential application as drug delivery systems and by the necessity to monitor their distribution in cells and tissues. (nature.com)
  • First, our results showed that TE at the concentration of 1 g/ml could promote cell proliferation over various durations, induce a transition of the cells from the G1-phase to the S/G2-phase of the cell cycle, and increase the expression of cell cycle proteins (CyclinB1 and CyclinD1). (bireme.br)
  • The research, by scientists at McGill University in Montreal and the Radboud University Medical Center in the Netherlands, focuses on a complex of proteins, known as podosomes, found in the membrane of migratory cells and in certain invasive cancer cells. (mcgill.ca)
  • Here, we examined the effect of amitriptyline on protein clearance and its relevant mechanisms in neuronal cells. (nature.com)
  • Protein quality control is crucial for maintaining cellular homeostasis and the functionality of eukaryotic cells. (nature.com)
  • Figure 3: Cell extracts from unstimulated or stimulated NIH/3T3 cells were applied to wells at increasing total protein concentrations and analyzed. (cellsignal.com)
  • As shown in Figure 2, stimulation of NIH/3T3 cells with PDGF promotes phosphorylation of Akt1 at Ser473, S6 ribosomal protein at Ser235/236 and p44 MAP kinase at Thr202/Tyr204. (cellsignal.com)
  • This invention is directed towards methods of destabilizing proteins in living cells, and their use for the development of novel assays. (freepatentsonline.com)
  • Prolonged exposure to high glucose with or without insulin downregulated B cell lymphoma 2-associated X (Bax) and failed to enhance the expression of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (p38MAPK) in drug-treated cells. (hindawi.com)
  • After one passage, the cells were treated with 5 ng/ml of transforming growth factor (TGF)-β2 for 48 h, and the expression levels of α-smooth muscle actin (α-SMA), extracellular matrix proteins, and phosphorylated Smad3 were evaluated with western blotting. (molvis.org)
  • Construction of quantitative analysis system for protein-protein interaction inside animal-derived cells was attempted with controlling those protein expression levels using peptide nucleic acid (PNA)-peptide conjugates. (nii.ac.jp)
  • Bartosz, G. 2002-03-25 00:00:00 We employed human red blood cells as a model system to check the affinity of MRP1 (Multi-drug Resistance-associated Protein 1) towards fluorescein and a set of its carboxyl derivatives: 5/6-carboxyfluorescein (CF), 2′,7′-bis-(2-carboxyethyl)-5/6-carboxyfluorescein (BCECF) and calcein (CAL). (deepdyve.com)
  • Photosystem I (PSI) is a stable, abundant, and highly efficient photocatalytic protein that is found in photosynthetic organisms and has been utilized in solar cells and hydrogen fuel cells. (vanderbilt.edu)
  • LuxI is a Vibrio fischeri enzyme that catalyzes the synthesis of N -(3-oxohexanoyl)- l -homoserine lactone from an acyl-acyl carrier protein and S -adenosylmethionine. (asm.org)
  • A model for the synthesis of acyl-HSLs by the LuxI protein family. (asm.org)
  • The ability of the purified fusion protein to catalyze the synthesis of acyl-HSLs was examined. (asm.org)
  • A DNA transcription unit encoding for a protein contains not only the sequence that will eventually be directly translated into the protein (the coding sequence) but also regulatory sequences that direct and regulate the synthesis of that protein. (wikibooks.org)
  • Expression of small heat shock proteins HspB2, HspB8, Hsp20 and cvHsp in different tissues of the pe. (biomedsearch.com)
  • Finally, we demonstrated that the TE-induced expression of cell cycle proteins was decreased by ERK1/2 inhibitor PD98059 but not by PI3K inhibitor LY294002 or JNK inhibitor SP600125. (bireme.br)
  • The reduced complexity of protein production in cell-free expression systems results in a frequent correlation of efficiency problems with the essential transcription/translation process. (jascoinc.com)
  • We present a systematic tag variation strategy for the rapid improvement of cell-free expression efficiencies of membrane proteins based on the optimization of translation initiation. (jascoinc.com)
  • A small number of rationally designed short expression tags is attached via overlap PCR to the 5-prime end of the target protein coding sequence. (jascoinc.com)
  • The generated pool of DNA templates is analyzed in a cell-free expression screen and the most efficient template is selected for further preparative scale protein production. (jascoinc.com)
  • The current study introduces a novel expression and purification system for human insulin production using the molecular chaperone αB-crystallin (αB-Cry) as a fusion partner protein. (jascoinc.com)
  • Overall, the novel expression system using αB-Cry is highly demanding for producing human insulin and functional protein. (jascoinc.com)
  • Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. (wikibooks.org)
  • The FREQUENCY (FRQ) protein is a central component of the Neurospora core clock, a transcription/translation negative feedback loop that controls genome-wide rhythmic gene expression. (genetics.org)
  • The first analyte is linked to a specific binding agent on a solid support such as a polymer and then another reaction couples the first poorly luminescent lanthanide complex with a new better one. (wikipedia.org)
  • This shows that BOP proteins act as substrate adaptors in a CUL3 BOP1/BOP2 E3 ubiquitin ligase complex, targeting PIF4 proteins for ubiquitination and subsequent degradation. (elifesciences.org)
  • Chemiluminescent HRP substrate is used to produce luminescent signal. (cellsignal.com)
  • RAS proteins, critical regulators of cell growth and differentiation, are the most frequently mutated oncogenes in humans. (pnas.org)
  • AinS does show similarity with the luxM gene product from the marine luminescent bacterium Vibrio harveyi ( 5 , 9 ). (asm.org)
  • If the gene transcribed encodes a protein, the result of transcription is messenger RNA (mRNA), which will then be used to create that protein via the process of translation. (wikibooks.org)
  • Alternatively, the transcribed gene may encode for either ribosomal RNA (rRNA) or transfer RNA (tRNA), other components of the protein-assembly process, or other ribozymes. (wikibooks.org)
  • With the avalanche of protein sequences generated in the postgenomic age, it is critical to develop computational methods for identifying in a timely fashion the protein-protein binding sites (PPBSs) based on the sequence information alone because the information obtained by this way can be used for both biomedical research and drug development. (mdpi.com)
  • The AinS protein shows no significant sequence similarity with LuxI family members, but it does show sequence similarity with the Vibrio harveyi LuxM protein. (asm.org)
  • Zinc finger protein is the major DNA binding protein in nature and it recognizes dsDNA with sequence specific manner. (omicsonline.org)
  • LuxU shows sequence similarity to other described phosphorelay proteins, including BvgS, ArcB, and Ypd1. (asm.org)
  • We report a luminescent micelle that is prepared through the self-assembly of an amphiphilic, neutral Pt( II ) complex with isoxazole moieties in THF/water on account of its aggregation-induced emission (AIE) property. (rsc.org)
  • Moreover, we found that BOP proteins physically interact with both PIF4 and CULLIN3A and that a CULLIN3-BOP2 complex ubiquitinates PIF4 in vitro. (elifesciences.org)
  • Lanthanides can be used because their small size (ionic radius) gives them the ability to replace metal ions inside protein complex such as calcium or nickel. (wikipedia.org)
  • We show that removal of the single protein subunit NDUFS4 prevents assembly of complex I and removes its function from mitochondria without pleiotropic effects on other respiratory components. (plantphysiol.org)
  • Thus, the luminescent SMPs offer a cheap and trackable alternative to existing materials for cellular internalisation of proteins, such as the HIV TAT protein and commercial protein delivery agents (e.g. (worktribe.com)
  • The cellular protein actin is linked to a luminescent protein. (mcgill.ca)
  • The accumulation of misfolded protein aggregates has been suggested to cause cellular toxicity and has been implicated in the common pathogenesis of neurodegenerative diseases. (nature.com)
  • Because it can attach itself to individual proteins, GFP is used as a "tag" allowing scientists to track all sorts of cellular processes under a microscope. (princeton.edu)
  • Since the linear range of ATP is extended to 500 µM, it is feasible to screen libraries for compounds that are not only competitive with ATP but also for those that are non competitive which broaden the selection of inhibitors of both serine/threonine protein kinases as well as tyrosine protein kinases. (aacrjournals.org)
  • The autoinducers are recognized by two-component hybrid sensor kinases called LuxN and LuxQ, and sensory information from both systems is transduced by a phosphorelay mechanism to the response regulator protein LuxO. (asm.org)
  • Amitriptyline interfered with the fusion of autophagosome and lysosome through the activation of PI3K/Akt/mTOR pathway and Beclin 1 acetylation, and regulated lysosome positioning by increasing the interaction between proteins Arl8, SKIP, and kinesin. (nature.com)
  • C-reactive protein is considered one of the best measures of the acute phase response to an infectious disease or other cause of tissue damage and inflammation. (cdc.gov)
  • This method quantified C- reactive protein (CRP) by latex-enhanced nephelometry. (cdc.gov)
  • C-reactive protein (CRP) Participants aged 16-69 years were tested. (cdc.gov)
  • Therefore, in the present study, we developed a very small probe for protein-protein interaction assay 1) . (titech.ac.jp)
  • This technology "NanoLuc ternary technology" could be a tool to analyze the interaction of such proteins. (titech.ac.jp)
  • In this study, the researchers propose a mechanism of interaction between zinc and protein with a synergy between the two partners. (cea.fr)
  • In addition, we have developed a simple purification strategy based on mixed-composition conjugates of the molecular adaptor and a second two-domain protein that allows the use of affinity chromatography. (nih.gov)
  • However, the difficulty of expressing and purifying functional membrane proteins has drastically hindered in-depth understanding of the molecular structures and physiological functions of these proteins. (mdpi.com)
  • This study may serve as a general guide for improving the yields of other membrane protein preparations and selecting the appropriate detergent to stabilize membrane proteins for biophysical and biochemical analyses. (mdpi.com)
  • LA JOLLA, CA, February 28, 2008-A chance discovery of a uniquely luminescent monoclonal antibody nearly ten years ago has proven to be far more interesting-and far more tenacious-than anyone might have suspected. (scripps.edu)
  • Luminescent conjugated oligothiophenes (LCOs) represent a useful and interesting class of materials well known for their abilities as transducers for colorimetric and fluorometric reporting. (diva-portal.org)
  • LuxI is from the marine luminescent bacterium Vibrio fischeri . (asm.org)
  • Usually, quorum sensing is controlled by two regulatory proteins similar to LuxI and LuxR from V. fischeri . (asm.org)
  • The luminescent properties of organic nanocarriers are usually associated with the native material emission characteristics or are the result of their labeling with emissive moieties. (nature.com)
  • operatively coupling a target protein to a linear multimerized destabilization domain, wherein said linear multimerized destabilization domain is non-cleavable by a α-NH-ubiquitin protein endoproteases, and comprises at least two copies of a destabilization domain. (freepatentsonline.com)
  • 8 . The process of claim 7 , wherein said ENZ66 preparation comprises an isolated ENZ66 protein, polypeptide or peptide. (google.es)
  • A sensing element is attached to the distal end of the optical conduit, and comprises at least one binding protein adapted to bind with at least one target analyte. (google.com)
  • 7 . The device of claim 1 , wherein the device comprises at least one reference group, and wherein the at least one reference group is associated with a protein. (google.com)
  • We foresee that these findings might aid in the chemical design of thiophene-based ligands that are increasingly selective for distinct disease-associated protein aggregates. (diva-portal.org)
  • SPC signaling has been found to involve intracellular signaling components such as pertussis toxin (PTX)-sensitive G proteins, which act in processes such as chemotactic migration ( 12 ). (jimmunol.org)