A photoprotein isolated from the bioluminescent jellyfish Aequorea. It emits visible light by an intramolecular reaction when a trace amount of calcium ion is added. The light-emitting moiety in the bioluminescence reaction is believed to be 2-amino-3-benzyl-5-(p-hydroxyphenyl)pyrazine (AF-350).
Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.
Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.

Mitochondrial depolarization accompanies cytochrome c release during apoptosis in PC6 cells. (1/8843)

Cytochrome c is released from mitochondria into the cytosol in cells undergoing apoptosis. The temporal relationship between cytochrome c release and loss of mitochondrial membrane potential was monitored by laser-scanning confocal microscopy in single living pheochromocytoma-6 cells undergoing apoptosis induced by staurosporine. Mitochondrial membrane potential monitored by tetramethylrhodamine methyl ester decreased abruptly in individual cells from 2 to 7 h after treatment with staurosporine. Depolarization was accompanied by cytochrome c release documented by release of transfected green fluorescent protein-tagged cytochrome c in these cells. The results show that mitochondrial depolarization accompanies cytochrome c release in pheochromocytoma-6 cells undergoing apoptosis.  (+info)

The dually acylated NH2-terminal domain of gi1alpha is sufficient to target a green fluorescent protein reporter to caveolin-enriched plasma membrane domains. Palmitoylation of caveolin-1 is required for the recognition of dually acylated g-protein alpha subunits in vivo. (2/8843)

Here we investigate the molecular mechanisms that govern the targeting of G-protein alpha subunits to the plasma membrane. For this purpose, we used Gi1alpha as a model dually acylated G-protein. We fused full-length Gi1alpha or its extreme NH2-terminal domain (residues 1-32 or 1-122) to green fluorescent protein (GFP) and analyzed the subcellular localization of these fusion proteins. We show that the first 32 amino acids of Gi1alpha are sufficient to target GFP to caveolin-enriched domains of the plasma membrane in vivo, as demonstrated by co-fractionation and co-immunoprecipitation with caveolin-1. Interestingly, when dual acylation of this 32-amino acid domain was blocked by specific point mutations (G2A or C3S), the resulting GFP fusion proteins were localized to the cytoplasm and excluded from caveolin-rich regions. The myristoylated but nonpalmitoylated (C3S) chimera only partially partitioned into caveolin-containing fractions. However, both nonacylated GFP fusions (G2A and C3S) no longer co-immunoprecipitated with caveolin-1. Taken together, these results indicate that lipid modification of the NH2-terminal of Gi1alpha is essential for targeting to its correct destination and interaction with caveolin-1. Also, a caveolin-1 mutant lacking all three palmitoylation sites (C133S, C143S, and C156S) was unable to co-immunoprecipitate these dually acylated GFP-G-protein fusions. Thus, dual acylation of the NH2-terminal domain of Gi1alpha and palmitoylation of caveolin-1 are both required to stabilize and perhaps regulate this reciprocal interaction at the plasma membrane in vivo. Our results provide the first demonstration of a functional role for caveolin-1 palmitoylation in its interaction with signaling molecules.  (+info)

Properties of filament-bound myosin light chain kinase. (3/8843)

Myosin light chain kinase binds to actin-containing filaments from cells with a greater affinity than to F-actin. However, it is not known if this binding in cells is regulated by Ca2+/calmodulin as it is with F-actin. Therefore, the binding properties of the kinase to stress fibers were examined in smooth muscle-derived A7r5 cells. Full-length myosin light chain kinase or a truncation mutant lacking residues 2-142 was expressed as chimeras containing green fluorescent protein at the C terminus. In intact cells, the full-length kinase bound to stress fibers, whereas the truncated kinase showed diffuse fluorescence in the cytoplasm. After permeabilization with saponin, the fluorescence from the truncated kinase disappeared, whereas the fluorescence of the full-length kinase was retained on stress fibers. Measurements of fluorescence intensities and fluorescence recovery after photobleaching of the full-length myosin light chain kinase in saponin-permeable cells showed that Ca2+/calmodulin did not dissociate the kinase from these filaments. However, the filament-bound kinase was sufficient for Ca2+-dependent phosphorylation of myosin regulatory light chain and contraction of stress fibers. Thus, dissociation of myosin light chain kinase from actin-containing thin filaments is not necessary for phosphorylation of myosin light chain in thick filaments. We note that the distance between the N terminus and the catalytic core of the kinase is sufficient to span the distance between thin and thick filaments.  (+info)

A fluorescent orthotopic bone metastasis model of human prostate cancer. (4/8843)

Here, we report a fluorescent spontaneous bone metastatic model of human prostate cancer developed by surgical orthotopic implantation of green fluorescent protein (GFP)-expressing prostate cancer tissue. Human prostate cancer PC-3 cells were transduced with the pLEIN expression retroviral vector containing the enhanced GFP and neomycin resistance genes. Stable GFP high-expression PC-3 clones were selected in vitro with G418, which were then combined and injected s.c. in nude mice. For metastasis studies, fragments of a single highly fluorescent s.c. growing tumor were implanted by surgical orthotopic implantation in the prostate of a series of nude mice. Subsequent micrometastases and metastases were visualized by GFP fluorescence throughout the skeleton, including the skull, rib, pelvis, femur, and tibia The central nervous system, including the brain and spinal cord, was also involved with tumor, as visualized by GFP fluorescence. Systemic organs, including the lung, plural membrane, liver, kidney, and adrenal gland, also had fluorescent metastases. The metastasis pattern in this model reflects the bone and other metastatic sites of human prostate cancer. Thus, this model should be very useful for the study and development of treatment for metastatic androgen-independent prostate cancer.  (+info)

Role of cytokine signaling molecules in erythroid differentiation of mouse fetal liver hematopoietic cells: functional analysis of signaling molecules by retrovirus-mediated expression. (5/8843)

Erythropoietin (EPO) and its cell surface receptor (EPOR) play a central role in proliferation, differentiation, and survival of erythroid progenitors. Signals induced by EPO have been studied extensively by using erythroid as well as nonerythroid cell lines, and various controversial results have been reported as to the role of signaling molecules in erythroid differentiation. Here we describe a novel approach to analyze the EPO signaling by using primary mouse fetal liver hematopoietic cells to avoid possible artifacts due to established cell lines. Our strategy is based on high-titer retrovirus vectors with a bicistronic expression system consisting of an internal ribosome entry site (IRES) and green fluorescent protein (GFP). By placing the cDNA for a signaling molecule in front of IRES-GFP, virus-infected cells can be viably sorted by fluorescence-activated cell sorter, and the effect of expression of the signaling molecule can be assessed. By using this system, expression of cell-survival genes such as Bcl-2 and Bcl-XL was found to enhance erythroid colony formation from colony-forming unit-erythroid (CFU-E) in response to EPO. However, their expression was not sufficient for erythroid colony formation from CFU-E alone, indicating that EPO induces signals for erythroid differentiation. To examine the role of EPOR tyrosine residues in erythroid differentiation, we introduced a chimeric EGFR-EPOR receptor, which has the extracellular domain of the EGF receptor and the intracellular domain of the EPOR, as well as a mutant EGFR-EPOR in which all the cytoplasmic tyrosine residues are replaced with phenylalanine, and found that tyrosine residues of EPOR are essential for erythroid colony formation from CFU-E. We further analyzed the function of the downstream signaling molecules by expressing modified signaling molecules and found that both JAK2/STAT5 and Ras, two major signaling pathways activated by EPOR, are involved in full erythroid differentiation.  (+info)

Nuclear translocation of green fluorescent protein-nuclear factor kappaB with a distinct lag time in living cells. (6/8843)

A highly fluorescent mutant form of the green fluorescent protein (GFP) has been fused to the human nuclear factor kappaB (NF-kappaB) p50 and p105 (p50/IkappaB gamma), a precursor protein of NF-kappaB p50. GFP-p50 and GFP-p105 were expressed in monkey COS-7 cells and human HeLa cells. Translocation of these chimeric proteins was observed by confocal laser scanning microscopy. GFP-p50 (without IkappaB gamma) in the transfected cells resided in the nucleus. On the other hand, GFP-p105 (GFP-p50 with IkappaB gamma) localized only in the cytoplasm before stimulation and translocated to the nucleus with stimulant specificity similar to that of native NF-kappaB/IkappaB. In addition, the translocation of NF-kappaB to the nucleus had a distinct lag time (a quiescent time) in the target cells. The lag time lasted 10-20 min after stimulation with hydrogen peroxide or tumor necrosis factor alpha. It was suggested that this might be due to the existence of a limiting step where NF-kappaB is released from NF-kappaB/IkappaB by the proteasome.  (+info)

Microtubule-dependent plus- and minus end-directed motilities are competing processes for nuclear targeting of adenovirus. (7/8843)

Adenovirus (Ad) enters target cells by receptor-mediated endocytosis, escapes to the cytosol, and then delivers its DNA genome into the nucleus. Here we analyzed the trafficking of fluorophore-tagged viruses in HeLa and TC7 cells by time-lapse microscopy. Our results show that native or taxol-stabilized microtubules (MTs) support alternating minus- and plus end-directed movements of cytosolic virus with elementary speeds up to 2.6 micrometer/s. No directed movement was observed in nocodazole-treated cells. Switching between plus- and minus end-directed elementary speeds at frequencies up to 1 Hz was observed in the periphery and near the MT organizing center (MTOC) after recovery from nocodazole treatment. MT-dependent motilities allowed virus accumulation near the MTOC at population speeds of 1-10 micrometer/min, depending on the cell type. Overexpression of p50/dynamitin, which is known to affect dynein-dependent minus end-directed vesicular transport, significantly reduced the extent and the frequency of minus end-directed migration of cytosolic virus, and increased the frequency, but not the extent of plus end-directed motility. The data imply that a single cytosolic Ad particle engages with two types of MT-dependent motor activities, the minus end- directed cytoplasmic dynein and an unknown plus end- directed activity.  (+info)

Estimation of the number of alpha-helical and beta-strand segments in proteins using circular dichroism spectroscopy. (8/8843)

A simple approach to estimate the number of alpha-helical and beta-strand segments from protein circular dichroism spectra is described. The alpha-helix and beta-sheet conformations in globular protein structures, assigned by DSSP and STRIDE algorithms, were divided into regular and distorted fractions by considering a certain number of terminal residues in a given alpha-helix or beta-strand segment to be distorted. The resulting secondary structure fractions for 29 reference proteins were used in the analyses of circular dichroism spectra by the SELCON method. From the performance indices of the analyses, we determined that, on an average, four residues per alpha-helix and two residues per beta-strand may be considered distorted in proteins. The number of alpha-helical and beta-strand segments and their average length in a given protein were estimated from the fraction of distorted alpha-helix and beta-strand conformations determined from the analysis of circular dichroism spectra. The statistical test for the reference protein set shows the high reliability of such a classification of protein secondary structure. The method was used to analyze the circular dichroism spectra of four additional proteins and the predicted structural characteristics agree with the crystal structure data.  (+info)

Aequorin is a bioluminescent protein found in certain jellyfish species, such as Aequorea victoria. It emits light when it undergoes a conformational change in the presence of calcium ions (Ca^2+^). This property makes aequorin a valuable tool in studying intracellular calcium levels and dynamics in various biological systems, including cells and model organisms.

The reaction that leads to light emission involves the binding of Ca^2+^ ions to aequorin, which then triggers the oxidation of coelenterazine, a chromophore molecule, to produce coelenteramide along with the release of energy in the form of blue light (approximately 469 nm). The intensity of the light emitted is directly proportional to the concentration of Ca^2+^ ions, allowing researchers to monitor and measure calcium levels in real-time.

Aequorin has been widely used in various research fields, such as neuroscience, cardiology, and cell biology, to investigate calcium signaling pathways and their roles in numerous physiological processes and diseases. Additionally, aequorin-based biosensors have been developed to study calcium dynamics in vivo, providing valuable insights into the complex interplay between calcium homeostasis and cellular functions.

Luminescent measurements refer to the quantitative assessment of the emission of light from a substance that has been excited, typically through some form of energy input such as electrical energy or radiation. In the context of medical diagnostics and research, luminescent measurements can be used in various applications, including bioluminescence imaging, which is used to study biological processes at the cellular and molecular level.

Bioluminescence occurs when a chemical reaction produces light within a living organism, often through the action of enzymes such as luciferase. By introducing a luciferase gene into cells or organisms, researchers can use bioluminescent measurements to track cellular processes and monitor gene expression in real time.

Luminescent measurements may also be used in medical research to study the properties of materials used in medical devices, such as LEDs or optical fibers, or to develop new diagnostic tools based on light-emitting nanoparticles or other luminescent materials.

In summary, luminescent measurements are a valuable tool in medical research and diagnostics, providing a non-invasive way to study biological processes and develop new technologies for disease detection and treatment.

Luminescent proteins are a type of protein that emit light through a chemical reaction, rather than by absorbing and re-emitting light like fluorescent proteins. This process is called bioluminescence. The light emitted by luminescent proteins is often used in scientific research as a way to visualize and track biological processes within cells and organisms.

One of the most well-known luminescent proteins is Green Fluorescent Protein (GFP), which was originally isolated from jellyfish. However, GFP is actually a fluorescent protein, not a luminescent one. A true example of a luminescent protein is the enzyme luciferase, which is found in fireflies and other bioluminescent organisms. When luciferase reacts with its substrate, luciferin, it produces light through a process called oxidation.

Luminescent proteins have many applications in research, including as reporters for gene expression, as markers for protein-protein interactions, and as tools for studying the dynamics of cellular processes. They are also used in medical imaging and diagnostics, as well as in the development of new therapies.

The result is luminescent. A chemiluminescent microparticle immunoassay uses magnetic, protein-coated microparticles. ... A plate is coated with a viral protein, such as a SARS-CoV-2 spike protein. Samples are incubated with the protein, allowing ... Antigen tests look for antigen proteins from the viral surface. In the case of a coronavirus, these are usually proteins from ... "A SARS-CoV-2 surrogate virus neutralization test based on antibody-mediated blockage of ACE2-spike protein-protein interaction ...
"Luminescent Eel Muscles Fluorescent Protein Revolution into Clinic." Huffington Post, June 18, 2013. "Mending Broken Hearts: ... "From CRISPR to glowing proteins to optogenetics - scientists' most powerful technologies have been borrowed from nature." The ... "AI makes huge progress predicting how proteins fold - one of biology's greatest challenges - promising rapid drug development ...
A way around this problem is with luminescent tags. These tags are quantum dots attached to proteins that penetrate cell ... These devices are built with nanowires to detect cancer proteins; each nanowire detector is primed to be sensitive to a ... Binding agents such as proteins, antibiotics, or synthetic ligands are covalently linked to the particle surface. These binding ... able to detect proteins and other biomarkers left behind by cancer cells, could enable the detection and diagnosis of cancer in ...
Turro, C; Fu, PK; Bradley, PM (2003). "Lanthanide ions as luminescent probes of proteins and nucleic acids". Metal Ions in ... polyaminocarboxylates as luminescent sensors in time-resolved luminescent (TRL) immunoassays. Optimization of analytical ... The luminescent probe may for instance serve to localize the MRI contrast agent. This has helped to visualize the delivery of ... LRET was also used to study the interaction of the proteins dystrophin and actin in muscle cells. Dystrophin is present in the ...
"CRISPR-Mediated Tagging of Endogenous Proteins with a Luminescent Peptide". ACS Chemical Biology. 13 (2): 467-474. doi:10.1021/ ... Protein tags are peptide sequences genetically grafted onto a recombinant protein. Tags are attached to proteins for various ... a protein which binds to immobilized glutathione Green fluorescent protein-tag, a protein which is spontaneously fluorescent ... This tag is used for protein purification of recombinant proteins and its fragments. It can be used in research labs and it is ...
Permyakov, Eugene A. [Luminescent Spectroscopy of Proteins], CRC Press, 1993. Fluorescence lifetimes and dynamic quenching ( ...
"Cloning and sequence analysis of cDNA for the luminescent protein aequorin". Proc. Natl. Acad. Sci. U.S.A. 82 (10): 3154-58. ... "Prediction of EF-hand calcium-binding proteins and analysis of bacterial EF-hand proteins". Proteins. 65 (3): 643-655. doi: ... Notably, the protein contains three EF hand motifs that function as binding sites for Ca2+ ions. The protein is a member of the ... In the animal, the protein occurs together with the green fluorescent protein to produce green light by resonant energy ...
Bioluminescence imaging - a technique for studying laboratory animals using luminescent protein. Calcium imaging - determining ...
A number of atomically precise luminescent clusters have been made in proteins and their growth involves inter-protein metal ... "Understanding the Evolution of Luminescent Gold Quantum Clusters in Protein Templates". ACS Nano. 5 (11): 8816-8827. doi: ... Combining luminescent atomically precise clusters with mesoflowers and nanofibres, he developed sensors at sub-zeptomole levels ... 4. Noble metal clusters in protein templates, T. Pradeep, A. Baksi and P. L. Xavier in Functional nanometer-sized clusters of ...
Recently, chimeras of RLuc have been developed and demonstrated to be the brightest luminescent proteins to date, and have ... "Luminescent proteins for high-speed single-cell and whole-body imaging". Nature Communications. 3: 1262. Bibcode:2012NatCo... ... Protein Engineering. 5 (3): 197-211. doi:10.1093/protein/5.3.197. hdl:11370/2d4c057d-1a67-437d-ad10-701f7a60f1e6. PMID 1409539 ... Protein Engineering, Design & Selection. 19 (9): 391-400. doi:10.1093/protein/gzl023. PMID 16857694. Anderson JM, Charbonneau H ...
"A Luminescent Ruthenium Complex for Ultrasensitive Detection of Proteins Immobilized on Membrane Supports". Analytical ... "A Luminescent Europium Complex for the Sensitive Detection of Proteins and Nucleic Acids Immobilized on Membrane Supports". ... is used as a protein dye in biochemistry for differentiating and detecting different proteins in laboratory settings. In recent ... "Metal Chelates as Reversible Stains for Detection of Electroblotted Proteins: Application to Protein Microsequencing and ...
"Luminescent quantum clusters of gold in transferrin family protein, lactoferrin exhibiting FRET" (PDF). Nanoscale. 2 (12): 2769 ... The fraction of protein extracted from milk, contains 3.3% RNA, but, the protein preferably binds to double-stranded DNA rather ... Occurrence of iron-containing red protein in bovine milk was reported as early as in 1939; however, the protein could not be ... optical absorption spectra and presence of two iron atoms per protein molecule. The protein was extracted from milk, contained ...
Lamture, JB; Wensel, TG (1995). "Intensely luminescent immunoreactive conjugates of proteins and dipicolinate-based polymeric ... phycoerythrin or green fluorescent protein). Alternatively, specific or general proteins, nucleic acids, lipids or small ... Other proteins are fluorescent but require a fluorophore cofactor, and hence can only be used in vitro; these are often found ... Several fluorescent protein exist in nature[citation needed], but the most important one as a research tool is Green ...
... used reporter genes that induce visually identifiable characteristics usually involve fluorescent and luminescent proteins. ... The mRNA is then translated into protein. It is important that both proteins be able to properly fold into their active ... Nordgren, I. K.; Tavassoli, A (2014). "A bidirectional fluorescent two-hybrid system for monitoring protein-protein ... A more complex use of reporter genes on a large scale is in two-hybrid screening, which aims to identify proteins that natively ...
In the 1960s and 1970s, GFP, along with the separate luminescent protein aequorin (an enzyme that catalyzes the breakdown of ... Protein methods, Recombinant proteins, Cell imaging, Protein imaging, Fluorescent proteins, Bioluminescence, Cnidarian proteins ... The natural protein has 238 amino acids. Its molecular mass is 27 kD. Therefore, fusing the GFP gene to the gene of a protein ... The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to light in the blue to ...
Martin Chalfie (professor, United States): for developing new methods for bioluminescent analysis using GFP luminescent protein ...
Amyloid beta (Aβ) is a small protein, most often 40 or 42 amino acids in length, that is released from a longer parent protein ... Klingstedt T; Nilsson KPR (2012). "Luminescent conjugated poly- and oligo-thiophenes: optical ligands for spectral assignment ... The misfolded proteins stick to one another, eventually stacking together to form oligomers that merge to make the amyloid ... The normal function of Aβ is not certain, but plaques arise when the protein misfolds and begins to accumulate in the brain by ...
... allows one to control the expression of envelope proteins. A frequently used protein is the glycoprotein G (VSV-G ... In cell culture, neutralized pseudoviruses will be prevented from infecting cells and producing the luminescent reporter gene ... Envelope proteins incorporated into the pseudovirus allow the virus to readily enter different cell types with the ... With this method, the foreign viral envelope proteins can be used to alter host tropism or increase or decrease the stability ...
The AtBAG6 protein is a CaM-binding protein that binds to CaM only in the absence of Ca2+ and not in the presence of it. AtBAG6 ... Kilhoffer MC, Demaille JG, Gerard D (July 1980). "Terbium as luminescent probe of calmodulin calcium-binding sites; domains I ... "Protein-peptide interaction studies demonstrate the versatility of calmodulin target protein binding". Protein and Peptide ... CaM binding proteins are also known to regulate reproductive development in plants. For instance, the CaM-binding protein ...
... s are a type of enzyme, made of protein, from bioluminescent organisms. They add to the function of the luciferins ... The term photoprotein was first used to describe the unusual chemistry of the luminescent system of Chaetopterus (a marine ... Polychaete worm). This was meant to distinguish them from other light-producing proteins because these do not exhibit the usual ...
These involve labelled proteins targeted at biomarkers, nucleic acid sequences present within cells that are found when the ... The isolate is then exposed to fluorescent dye, which will be luminescent when viewed. Improvements to existing platforms are ...
Extraction, partial purification and properties of obelin, the calcium- activated luminescent protein from the hydroid Obelia ... He is an expert on bioluminescence, having developed the use of genetically engineered bioluminescent proteins to measure ... pioneering the application of Ca2+-activated bioluminescent proteins to measure free Ca2+ in live animal, plant, bacterial, and ...
All these proteins catalyze the oxidation of this substance, a reaction catalogued EC 1.13.12.5. Coelenterazine was ... Both groups independently discovered that the same compound was used in both luminescent systems. The molecule was named after ... Myctophidae echinoderms such as Amphiura filiformis The compound has also been isolated from organisms that are not luminescent ... simultaneously isolated and characterized by two groups studying the luminescent organisms sea pansy (Renilla reniformis) and ...
There, he continued his research on heme proteins, studying ligand migration within these proteins and its effects on the ... He has also been engaged in the development and characterization of nanoscale luminescent markers for bioimaging (fluorescent ... his laboratory carried on with studies of ligand binding and protein dynamics, mainly on heme proteins. Since 1997, he has been ... "Structural basis for photo-induced protein cleavage and green-to-red conversion of fluorescent protein EosFP". Proceedings of ...
The DNA encoding the luminescent protein is incorporated into the laboratory animal either via a viral vector or by creating a ... and green fluorescent protein for continuous imaging of cell culture and animal models". Journal of Biomedical Optics. 16 (4): ...
Furthermore, the chromatophores contain luminescent protein nanostructures in which tethered pigment granules modify light ... The blood of a cuttlefish is an unusual shade of green-blue, because it uses the copper-containing protein haemocyanin to carry ... "How Egg Case Proteins Can Protect Cuttlefish Offspring?". PLOS ONE. 10 (7): e0132836. Bibcode:2015PLoSO..1032836C. doi:10.1371/ ... oxygen instead of the red, iron-containing protein haemoglobin found in vertebrates' blood. The blood is pumped by three ...
Protein tag Green NM (1975). "Avidin". Advances in Protein Chemistry. 29: 85-133. doi:10.1016/s0065-3233(08)60411-8. ISBN ... Ostojic GN, Hersam MC (June 2012). "Biomolecule-directed assembly of self-supported, nanoporous, conductive, and luminescent ... Sinclair JC, Davies KM, Vénien-Bryan C, Noble ME (July 2011). "Generation of protein lattices by fusing proteins with matching ... Protein articles without symbol, CS1: long volume value, Bacterial proteins). ...
The adenovirus E1B-55K protein and the hepatitis B virus HBx protein are examples of viral proteins that can perform such a ... Luminescent iridium complex-peptide hybrids (IPHs) have recently been designed, which mimic TRAIL and bind to death receptors ... Examples of viral Bcl-2 proteins include the Epstein-Barr virus BHRF1 protein and the adenovirus E1B 19K protein. Some viruses ... these inhibitory proteins target retinoblastoma tumor-suppressing proteins. These tumor-suppressing proteins regulate the cell ...
Unlike GFPs, which are derived from the luminescent jellyfish Aequorea victoria, fluorescent proteins derived from anthozoa, ... of proteins within living cells while retaining complex biological functions like protein-protein interactions and protein-DNA ... Many different fusion proteins have been created using EosFP and its engineered variants. These fusion proteins allow for the ... Like other fluorescent proteins, Eos allows for applications such as the tracking of fusion proteins, multicolour labelling and ...
Branda SS, Chu F, Kearns DB, Losick R, Kolter R (February 2006). "A major protein component of the Bacillus subtilis biofilm ... 23 November 2016). "Salmonella biofilms using luminescent oligothiophenes". npj Biofilms and Microbiomes. 2: 16024. doi:10.1038 ... The EPS matrix consists of exopolysaccharides, proteins and nucleic acids. A large proportion of the EPS is more or less ... This means that the genes necessary for the production of proteins that work towards defending the plant against pathogens have ...
Tau Protein Binding Modes in Alzheimers Disease for Cationic Luminescent Ligands. * No Comments ... Figure caption: Luminescent conjugated oilgothiphenes are developed for early-stage detection of Alzheimers disease. The ... It is conclusively shown that the ligand-protein binding occurs at the hydrophobic pocket defined by residues Ile360, Thr361, ... Tau Protein Binding Modes in Alzheimers Disease for Cationic Luminescent Ligands. Phys. Chem. B 125, 11628 (2021); https://doi ...
A High-Resolution Luminescent Assay for Rapid and Continuous Monitoring of Protein Translocation across Biological Membranes. ... A High-Resolution Luminescent Assay for Rapid and Continuous Monitoring of Protein Translocation across Biological Membranes. ... A High-Resolution Luminescent Assay for Rapid and Continuous Monitoring of Protein Translocation across Biological Membranes. ... A High-Resolution Luminescent Assay for Rapid and Continuous Monitoring of Protein Translocation across Biological Membranes. ...
Fluorescent reporter proteins in the Drosophila model system offer a degree of specificity that allows monitoring cellular and ... Therefore, this chapter will show exciting results of the group using fluorescent proteins and nanocrystals in biological ... This book chapter will comment on fluorescent reporter proteins and nanocrystals applicability as fluorescent markers. ... This book chapter will comment in particular on green fluorescent protein and luminescent nanocrystals. ...
Luminescent Proteins / genetics * Photoreceptor Cells / cytology * Photoreceptor Cells / metabolism * Promoter Regions, Genetic ... An HIV vector containing a gene encoding the green fluorescent protein (GFP) was injected into the subretinal space of rat eyes ...
As is the case for other G protein-coupled … ... Luminescent Proteins / genetics * Luminescent Proteins / ... As is the case for other G protein-coupled receptors, chemokine receptors are not isolated entities that are activated ...
Structure of the Yellow Fluorescent Protein Citrine Frozen at 4000 Atmospheres Number 3: Structure 25 in a Series of 26 High ... Classification: LUMINESCENT PROTEIN. *Organism(s): Aequorea victoria. *Expression System: Escherichia coli. *Mutation(s): Yes ... because they suggest methods to tune protein function by modification of the protein scaffold. ... The protein has been compressed to produce deformations of its chromophore by applying a high-pressure cryocooling technique. A ...
The result is luminescent. A chemiluminescent microparticle immunoassay uses magnetic, protein-coated microparticles. ... A plate is coated with a viral protein, such as a SARS-CoV-2 spike protein. Samples are incubated with the protein, allowing ... Antigen tests look for antigen proteins from the viral surface. In the case of a coronavirus, these are usually proteins from ... "A SARS-CoV-2 surrogate virus neutralization test based on antibody-mediated blockage of ACE2-spike protein-protein interaction ...
Internalization of a preformed atomically precise silver cluster in proteins by multistep events and emergence of luminescent ... Internalization of a preformed atomically precise silver cluster in proteins by multistep events and emergence of luminescent ...
University of Basel researchers have reached an important milestone in their quest to produce more sustainable luminescent ... Using solid-state nanopores and DNA barcoding to identify misfolded proteins in neurodegenerative disorders. Dec 5, 2023 ... Manganese could make luminescent materials and the conversion of sunlight more sustainable. by University of Basel ... Manganese could make luminescent materials and the conversion of sunlight more sustainable. ...
Proteins and Peptides. Proteomics tools. Agonists, activators, antagonists and inhibitors. Cell lines and Lysates. Multiplex ...
Here we show that high-mobility group AT-hook 2 protein (HMGA2) induces DNA nicks at the transcription start site, which are ... However, the order of events and the precise function of high-mobility group proteins during transcription initiation remain ... of which the high-mobility group proteins are the most abundant. Chromatin-mediated regulation of transcription involves DNA ... Signals were detected and analyzed with Luminescent Image Analyzer (Las 4000, Fujifilm). Protein concentrations were determined ...
The F1/S and A genetic variants of the protein bind ellipticine with comparable affinity albeit the distinct topography of ... Dimeric binding of plant alkaloid ellipticine to human serum proteins. By Ferenc Zsila, Tamás Beke-Somfai ... Home / KnowledgeBase Articles / Dimeric binding of plant alkaloid ellipticine to human serum proteins ... Taking advantage of the ability of chiral protein environments to induce optical activity, circular dichroism (CD) spectroscopy ...
Submillisecond protein folding kinetics studied by ultrarapid mixing. By Chi-Kin Chan, Yi Hu, Satoshi Takahashi, Denis L. ... The results raise several fundamental issues concerning the dynamics of collapse and barrier crossings in protein folding. ... An ultrarapid-mixing continuous-flow method has been developed to study submillisecond folding of chemically denatured proteins ...
The cells were then homogenized at 4 °C, and the total protein concentration in 0.1 M NaOH extract was calculated. Protein ... Stable luminescent signals are correlated with either the GSH or GSSG concentration of a sample. In this method, the GSH- ... Determination of Total Protein Concentration. The MDA-MB-231 and ZR-75-1 cells (2.5 × 105 cells/mL) were incubated in 2 mL of ... Lowry, O.H.; Rosebrouugh, N.J.; Far, A.L.; Randal, R.J. Protein mesaurement with the Folin phenol reagent. J. Biol. Chem. 1951 ...
Amino Acids, Peptides, and Proteins [D12]. *Proteins [D12.776]. *Luminescent Proteins [D12.776.532] ... Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms ...
The bound HRP conjugate is then measured through the addition of a luminescent substrate. This signal reagent contains the ... C-reactive protein(mg/dL). English Text: C-reactive protein (mg/dL). Target: Both males and females 3 YEARS - 150 YEARS. Code ... C-reactive protein C-reactive protein is considered one of the best measures of the acute phase response to an infectious ... C-reactive protein This method quantified C-reactive protein (CRP) by latex-enhanced nephelometry. Particle-enhanced assays ...
Green Fluorescent Proteins; Luminescent Proteins; Mutagenesis; RNA, Viral; Replicon. ...
The amplified luminescent proximity homogeneous assay (AlphaScreen) identifies the interaction of HIV-1 Gag with PRKCI. PubMed ... General protein information Go to the top of the page Help Preferred Names. protein kinase C iota type. Names. PRKC-lambda/iota ... Protein interactions. Protein. Gene. Interaction. Pubs. Envelope surface glycoprotein gp120 env HIV-1 gp120 upregulates the ... mRNA and Protein(s) * XM_047448574.1 → XP_047304530.1 protein kinase C iota type isoform X1 ...
Structure of lumazine protein, an optical transponder of luminescent bacteria. Journal of Molecular Biology 382 (1), pp. 44 - ... and pro-aggregation variants to assess the effects of methionine oxidation in human prion protein. Proceedings of the National ... Interplay of PDZ and protease domain of DegP ensures efficient elimination of misfolded proteins. Proceedings of the National ...
Multiplexed immunoassays for proteins using magnetic luminescent nanoparticles for internal calibration. Authors. ...
Biologist David Gruber studies radiant creatures and their fluorescent proteins. ... He is purifying its fluorescent proteins and hopes they can someday be used as luminescent tags in biomedical research. ... Researchers can introduce these glowing proteins into cells to track all kinds of activity, including the growth of tumors or ... Few scientists knew the complete history of fluorescent protein research, so he and Pieribone wrote a book about it. ...
A Single-Component Luminescent Biosensor for the SARS-CoV-2 Spike Protein. Journal of the American Chemical Society Ravalin, M ... In particular, we focus on the use of PL for mapping protein-protein, protein-RNA and protein-DNA interactions in living cells ... We report a new method for detection of protein-protein interactions in vitro and in cells. One protein partner is fused to ... Protein-protein interaction detection in vitro and in cells by proximity biotinylation JOURNAL OF THE AMERICAN CHEMICAL SOCIETY ...
HSP, heat shock protein; MCAO, middle cerebral artery occlusion; GFAP, glial fibrillary acidic protein IBA1, ionized calcium- ... The PVDF membrane was incubated with the luminescent agent (cat no. SQ202, EpiZyme, Inc.) for a sufficient period and then ... Heat shock protein (HSP) 90 is abundant in cells, mainly in the form of homodimers, including HSP90α and HSP90β. As a molecular ... Qi J, Liu Y, Yang P, Chen T, Liu XZ, Yin Y, Zhang J and Wang F: Heat shock protein 90 inhibition by 17-dimethylaminoethylamino- ...
We have developed a novel method for co-expressing multiple chimeric fluorescent fusion proteins in plants to overcome the ... Iridium(III) Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II… ... Iridium(III) Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II… ... Journal / Chemistry / Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants… ...
We also found two RanBP2 substrates, RanGTPase-activating protein and retinitis pigmentosa GTPase regulator interacting protein ... The ubiquitin-proteasome system (UPS) plays a critical role in protein degradation. The 19S regulatory particle (RP) of the 26S ... Yi, H., Friedman, J. L., & Ferreira, P. A. (2007). The cyclophilin-like domain of Ran-binding protein-2 modulates selectively ... The cyclophilin-like domain of Ran-binding protein-2 modulates selectively the activity of the ubiquitin-proteasome system and ...
We introduce the term protein connectivity-based dysfunction (PCBD) to define this mechanism. Among most sensitive to PCBD are ... We propose AD is a protein connectivity-based dysfunction disorder whereby a switch of the chaperome into epichaperomes rewires ... These structures provide the backbone upon which proteome-wide connectivity, and in turn, protein networks become disturbed and ... PU-AD was added to the cells for 72 h and the CellTiter-Glo Luminescent Cell Viability Assay (Promega) assay was performed ...
Luminescent reporter assays are powerful research tools for a variety of applications. This webinar explains critical aspects ... signal transduction and protein function. Luminescent reporters are also increasingly used for live, real-time measurement of ... Protein Purification Promotions Theres no time to waste! Applications for our $5,000 D.O.O.R.S. Scholarship close on September ... Luminescent reporter assays are widely used across scientific disciplines, and for good reason. Reporters can provide a highly ...
Journal Article] Rigid Luminescent Bis-Zinc(II)-Bis-Cyclen Complexes for the Detection of Phosphate Anions and Non-Covalent ... Presentation] Chemistry-based protein labeling and imaging in cell and in vivo2010. *. Author(s). Itaru Hamachi ... Presentation] Chemistry-based protein labeling and imaging in cell and in vivo2010. *. Author(s). Itaru Hamachi ... Journal Article] Quantitative comparison of protein dynamics in live cells and in vitro by in-cell (19)F-NMR.2013. *. Author(s) ...
Fluorescent protein. Proteins that emit fluorescence represented by green fluorescent protein (GFP). When a fluorescent protein ... When luciferase reacts with a specific luminescent substance, the enzymatic reaction proceeds and light of a particular ... We have created a transgenic P. falciparum that expresses two reporter proteins, GFP and NanoLuc, at high levels in both the ... This transgenic P. falciparum reporter line constantly expresses two reporter proteins, GFP and NanoLuc, at high levels in two ...

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