Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
Elements of limited time intervals, contributing to particular results or situations.
The failure by the observer to measure or identify a phenomenon accurately, which results in an error. Sources for this may be due to the observer's missing an abnormality, or to faulty technique resulting in incorrect test measurement, or to misinterpretation of the data. Two varieties are inter-observer variation (the amount observers vary from one another when reporting on the same material) and intra-observer variation (the amount one observer varies between observations when reporting more than once on the same material).
The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.
Determination, by measurement or comparison with a standard, of the correct value of each scale reading on a meter or other measuring instrument; or determination of the settings of a control device that correspond to particular values of voltage, current, frequency or other output.
Methods of creating machines and devices.
Observation of a population for a sufficient number of persons over a sufficient number of years to generate incidence or mortality rates subsequent to the selection of the study group.
A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.
A technique of inputting two-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer.
The process of generating three-dimensional images by electronic, photographic, or other methods. For example, three-dimensional images can be generated by assembling multiple tomographic images with the aid of a computer, while photographic 3-D images (HOLOGRAPHY) can be made by exposing film to the interference pattern created when two laser light sources shine on an object.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
The rate dynamics in chemical or physical systems.
In screening and diagnostic tests, the probability that a person with a positive test is a true positive (i.e., has the disease), is referred to as the predictive value of a positive test; whereas, the predictive value of a negative test is the probability that the person with a negative test does not have the disease. Predictive value is related to the sensitivity and specificity of the test.
The technique that deals with the measurement of the size, weight, and proportions of the human or other primate body.
A value equal to the total volume flow divided by the cross-sectional area of the vascular bed.
Techniques for measuring blood pressure.
A statistical technique that isolates and assesses the contributions of categorical independent variables to variation in the mean of a continuous dependent variable.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
Devices or objects in various imaging techniques used to visualize or enhance visualization by simulating conditions encountered in the procedure. Phantoms are used very often in procedures employing or measuring x-irradiation or radioactive material to evaluate performance. Phantoms often have properties similar to human tissue. Water demonstrates absorbing properties similar to normal tissue, hence water-filled phantoms are used to map radiation levels. Phantoms are used also as teaching aids to simulate real conditions with x-ray or ultrasonic machines. (From Iturralde, Dictionary and Handbook of Nuclear Medicine and Clinical Imaging, 1990)
Non-invasive method of demonstrating internal anatomy based on the principle that atomic nuclei in a strong magnetic field absorb pulses of radiofrequency energy and emit them as radiowaves which can be reconstructed into computerized images. The concept includes proton spin tomographic techniques.
A series of steps taken in order to conduct research.
Measurement of the amount of air that the lungs may contain at various points in the respiratory cycle.
PRESSURE of the BLOOD on the ARTERIES and other BLOOD VESSELS.
The evaluation of incidents involving the loss of function of a device. These evaluations are used for a variety of purposes such as to determine the failure rates, the causes of failures, costs of failures, and the reliability and maintainability of devices.
An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.
Statistical models in which the value of a parameter for a given value of a factor is assumed to be equal to a + bx, where a and b are constants. The models predict a linear regression.
A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy.
Procedures for finding the mathematical function which best describes the relationship between a dependent variable and one or more independent variables. In linear regression (see LINEAR MODELS) the relationship is constrained to be a straight line and LEAST-SQUARES ANALYSIS is used to determine the best fit. In logistic regression (see LOGISTIC MODELS) the dependent variable is qualitative rather than continuously variable and LIKELIHOOD FUNCTIONS are used to find the best relationship. In multiple regression, the dependent variable is considered to depend on more than a single independent variable.
Computer-based representation of physical systems and phenomena such as chemical processes.
Methods developed to aid in the interpretation of ultrasound, radiographic images, etc., for diagnosis of disease.
A type of stress exerted uniformly in all directions. Its measure is the force exerted per unit area. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Measurement of the intensity and quality of fluorescence.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.
The continuous measurement of physiological processes, blood pressure, heart rate, renal output, reflexes, respiration, etc., in a patient or experimental animal; includes pharmacologic monitoring, the measurement of administered drugs or their metabolites in the blood, tissues, or urine.
The closeness of a determined value of a physical dimension to the actual value.
The properties, processes, and behavior of biological systems under the action of mechanical forces.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
The deductive study of shape, quantity, and dependence. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Any device or element which converts an input signal into an output signal of a different form. Examples include the microphone, phonographic pickup, loudspeaker, barometer, photoelectric cell, automobile horn, doorbell, and underwater sound transducer. (McGraw Hill Dictionary of Scientific and Technical Terms, 4th ed)
The visualization of deep structures of the body by recording the reflections or echoes of ultrasonic pulses directed into the tissues. Use of ultrasound for imaging or diagnostic purposes employs frequencies ranging from 1.6 to 10 megahertz.
The resistance to the flow of either alternating or direct electrical current.
Theoretical representations that simulate the behavior or activity of systems, processes, or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.
Studies in which the presence or absence of disease or other health-related variables are determined in each member of the study population or in a representative sample at one particular time. This contrasts with LONGITUDINAL STUDIES which are followed over a period of time.
The monitoring of the level of toxins, chemical pollutants, microbial contaminants, or other harmful substances in the environment (soil, air, and water), workplace, or in the bodies of people and animals present in that environment.
Scales, questionnaires, tests, and other methods used to assess pain severity and duration in patients or experimental animals to aid in diagnosis, therapy, and physiological studies.
The diversion of RADIATION (thermal, electromagnetic, or nuclear) from its original path as a result of interactions or collisions with atoms, molecules, or larger particles in the atmosphere or other media. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The visualization of tissues during pregnancy through recording of the echoes of ultrasonic waves directed into the body. The procedure may be applied with reference to the mother or the fetus and with reference to organs or the detection of maternal or fetal disease.
Transducers that are activated by pressure changes, e.g., blood pressure.
Measuring and weighing systems and processes.
Tomography using x-ray transmission and a computer algorithm to reconstruct the image.
Studies in which individuals or populations are followed to assess the outcome of exposures, procedures, or effects of a characteristic, e.g., occurrence of disease.
An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (LASER) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum.
Levels within a diagnostic group which are established by various measurement criteria applied to the seriousness of a patient's disorder.
A colorless, odorless gas that can be formed by the body and is necessary for the respiration cycle of plants and animals.
An aspect of personal behavior or lifestyle, environmental exposure, or inborn or inherited characteristic, which, on the basis of epidemiologic evidence, is known to be associated with a health-related condition considered important to prevent.
The measurement of the dimensions of the HEAD.
Studies to determine the advantages or disadvantages, practicability, or capability of accomplishing a projected plan, study, or project.

Orally exhaled nitric oxide levels are related to the degree of blood eosinophilia in atopic children with mild-intermittent asthma. (1/3761)

Increased levels of nitric oxide have been found in expired air of patients with asthma, and these are thought to be related to the airway inflammatory events that characterize this disorder. Since, in adults, bronchial inflammatory changes are present even in mild disease, the present study was designed to evaluate whether a significant proportion of children with mild-intermittent asthma could have increased exhaled air NO concentrations. Twenty-two atopic children (aged 11.1+/-0.8 yrs) with mild-intermittent asthma, treated only with inhaled beta2-adrenoreceptor agonists on demand and 22 age-matched controls were studied. NO concentrations in orally exhaled air, measured by chemiluminescence, were significantly higher in asthmatics, as compared to controls (19.4+/-3.3 parts per billion (ppb) and 4.0+/-0.5 ppb, respectively; p<0.01). Interestingly, 14 out of 22 asthmatic children had NO levels >8.8 ppb (i.e. >2 standard deviations of the mean in controls). In asthmatic patients, but not in control subjects, statistically significant correlations were found between exhaled NO levels and absolute number or percentage of blood eosinophils (r=0.63 and 0.56, respectively; p<0.01, each comparison). In contrast, exhaled NO levels were not correlated with forced expiratory volume in one second (FEV1) or forced expiratory flows at 25-75% of vital capacity (FEF25-75%) or forced vital capacity (FVC), either in control subjects, or in asthmatic patients (p>0.1, each correlation). These results suggest that a significant proportion of children with mild-intermittent asthma may have airway inflammation, as shown by the presence of elevated levels of nitric oxide in the exhaled air. The clinical relevance of this observation remains to be established.  (+info)

Increased exhaled nitric oxide on days with high outdoor air pollution is of endogenous origin. (2/3761)

The aim of this study was to assess the effect of outdoor air pollution on exhaled levels of endogenously released nitric oxide. To exclude bias from exogenous NO in the recovered exhaled air (residual NO or NO in dead volume) an experimental design was used that sampled NO of endogenous origin only. The validity of the presented experimental design was established in experiments where subjects were exposed to high levels of exogenous NO (cigarette smoke or 480 microg x m(-3) synthetic NO). Subsequent 1 min breathing and a final inhalation of NO-free air proved to be sufficient to attain pre-exposure values. Using the presented method detecting only endogenous NO in exhaled air, 18 subjects were sampled on 4 separate days with different levels of outdoor air pollution (read as an ambient NO level of 4, 30, 138 and 246 microg x m(-3)). On the 2 days with highest outdoor air pollution, exhaled NO was significantly (p<0.001) increased (67-78%) above the mean baseline value assessed on 4 days with virtually no outdoor air pollution. In conclusion, the level of endogenous nitric oxide in exhaled air is increased on days with high outdoor air pollution. The physiological implications of this findings need to be investigated further.  (+info)

Induction of reactive oxygen intermediates in human monocytes by tumour cells and their role in spontaneous monocyte cytotoxicity. (3/3761)

The present study examined the ability of human monocytes to produce reactive oxygen intermediates after a contact with tumour cells. Monocytes generated oxygen radicals, as measured by luminol-enhanced chemiluminescence and superoxide anion production, after stimulation with the tumour, but not with untransformed, cells. The use of specific oxygen radical scavengers and inhibitors, superoxide dismutase, catalase, dimethyl sulphoxide and deferoxamine as well as the myeloperoxidase inhibitor 4-aminobenzoic acid hydrazide, indicated that chemiluminescence was dependent on the production of superoxide anion and hydroxyl radical and the presence of myeloperoxidase. The tumour cell-induced chemiluminescent response of monocytes showed different kinetics from that seen after activation of monocytes with phorbol ester. These results indicate that human monocytes can be directly stimulated by tumour cells for reactive oxygen intermediate production. Spontaneous monocyte-mediated cytotoxicity towards cancer cells was inhibited by superoxide dismutase, catalase, deferoxamine and hydrazide, implicating the role of superoxide anion, hydrogen peroxide, hydroxyl radical and hypohalite. We wish to suggest that so-called 'spontaneous' tumoricidal capacity of freshly isolated human monocytes may in fact be an inducible event associated with generation of reactive oxygen intermediates and perhaps other toxic mediators, resulting from a contact of monocytes with tumour cells.  (+info)

Rapid film-based determination of antibiotic susceptibilities of Mycobacterium tuberculosis strains by using a luciferase reporter phage and the Bronx Box. (4/3761)

Detecting antibiotic resistance in Mycobacterium tuberculosis is becoming increasingly important with the global recognition of drug-resistant strains and their adverse impact on clinical outcomes. Current methods of susceptibility testing are either time-consuming or costly; rapid, reliable, simple, and inexpensive methods would be highly desirable, especially in the developing world where most tuberculosis is found. The luciferase reporter phage is a unique reagent well-suited for this purpose: upon infection with viable mycobacteria, it produces quantifiable light which is not observed in mycobacterial cells treated with active antimicrobials. In this report, we describe a modification of our original assay, which allows detection of the emitted light with a Polaroid film box designated the Bronx Box. The technique has been applied to 25 M. tuberculosis reference and clinical strains, and criteria are presented which allow rapid and simple discrimination among strains susceptible or resistant to isoniazid and rifampin, the major antituberculosis agents.  (+info)

Evidence that halogenated furanones from Delisea pulchra inhibit acylated homoserine lactone (AHL)-mediated gene expression by displacing the AHL signal from its receptor protein. (5/3761)

Acylated homoserine lactone (AHL)-mediated gene expression controls phenotypes involved in colonization, often specifically of higher organisms, in both marine and terrestrial environments. The marine red alga Delisea pulchra produces halogenated furanones which resemble AHLs structurally and show inhibitory activity at ecologically realistic concentrations in AHL bioassays. Evidence is presented that halogenated furanones displace tritiated OHHL [N-3-(oxohexanoyl)-L-homoserine lactone] from Escherichia coli cells overproducing LuxR with potencies corresponding to their respective inhibitory activities in an AHL-regulated bioluminescence assay, indicating that this is the mechanism by which furanones inhibit AHL-dependent phenotypes. Alternative mechanisms for this phenomenon are also addressed. General metabolic disruption was assessed with two-dimensional PAGE, revealing limited non-AHL-related effects. A direct chemical interaction between the algal compounds and AHLs, as monitored by 1H NMR spectroscopy, was shown not to occur in vitro. These results support the contention that furanones, at the concentrations produced by the alga, can control bacterial colonization of surfaces by specifically interfering with AHL-mediated gene expression at the level of the LuxR protein.  (+info)

Reduction of serum cholesterol and hypercholesterolemic atherosclerosis in rabbits by secoisolariciresinol diglucoside isolated from flaxseed. (6/3761)

BACKGROUND: Secoisolariciresinol diglucoside (SDG) is a plant lignan isolated from flaxseed. Lignans are platelet-activating factor-receptor antagonists that would inhibit the production of oxygen radicals by polymorphonuclear leukocytes. SDG is an antioxidant. Antioxidants studied thus far are known to reduce hypercholesterolemic atherosclerosis. The objective of this study was to determine the effect of SDG on various blood lipid and aortic tissue oxidative stress parameters and on the development of atherosclerosis in rabbits fed a high-cholesterol diet. METHODS AND RESULTS: Rabbits were assigned to 4 groups: group 1, control; group 2, SDG control (15 mg. kg body wt-1. d-1 PO); group 3, 1% cholesterol diet; and group 4, same as group 3 but with added SDG (15 mg. kg body wt-1. d-1 PO). Blood samples were collected before (time 0) and after 4 and 8 weeks of experimental diets for measurement of serum triglycerides, total cholesterol (TC), and LDL, HDL, and VLDL cholesterol (LDL-C, HDL-C, and VLDL-C). The aorta was removed at the end of the protocol for assessment of atherosclerotic plaques; malondialdehyde, an aortic tissue lipid peroxidation product; and aortic tissue chemiluminescence, a marker for antioxidant reserve. Serum TC, LDL-C, and the ratios LDL-C/HDL-C and TC/HDL-C increased in groups 3 and 4 compared with time 0, the increase being smaller in group 4 than in group 3. Serum HDL-C decreased in group 3 and increased in group 4 compared with time 0, but changes were lower in group 3 than in group 4. SDG reduced TC and LDL-C by 33% and 35%, respectively, at week 8 but increased HDL-C significantly, by>140%, as early as week 4. It also decreased TC/LDL-C and LDL-C/HDL-C ratios by approximately 64%. There was an increase in aortic malondialdehyde and chemiluminescence in group 3, and they were lower in group 4 than in group 3. SDG reduced hypercholesterolemic atherosclerosis by 73%. CONCLUSIONS: These results suggest that SDG reduced hypercholesterolemic atherosclerosis and that this effect was associated with a decrease in serum cholesterol, LDL-C, and lipid peroxidation product and an increase in HDL-C and antioxidant reserve.  (+info)

Expression of T lymphocyte p56(lck), a zinc-finger signal transduction protein, is elevated by dietary zinc deficiency and diet restriction in mice. (7/3761)

Compromised immune function is common to Zn deficiency, protein and energy malnutrition; however, the causative mechanisms at the molecular level have not been elucidated. The T lymphocyte signal transduction pathway contains several Zn-finger proteins, and it is possible that the in vivo functioning of these proteins could be affected by dietary deficiency of Zn and amino acids. Thus, the objective was to investigate the effects, on expression of the T lymphocyte signal transduction proteins p56(lck), phospholipase Cgamma1 (PLCgamma1) and protein kinase C (PKCalpha), of dietary Zn deficiency (ZnDF, < 1 mg Zn/kg diet) and protein-energy malnutrition syndromes [2% protein deficiency (LP), combined Zn and 2% protein deficiency (ZnDF+LP), and diet restriction (DR, body weight equal to ZnDF)] compared with control (C) mice. Indices of nutritional status and splenocyte counts were also determined. Based on serum albumin and liver lipid concentrations, the ZnDF+LP and LP groups had protein-type malnutrition, whereas the ZnDF and DR groups had energy-type malnutrition. For Western immunoblotting of the signal transduction proteins, mouse splenic T lymphocytes were isolated by immunocolumns. The expression of T lymphocyte p56(lck) was significantly elevated in the ZnDF+LP, ZnDF and DR groups compared to the C group. In contrast, the expression of PLCgamma1 and PKC was unaffected. There was a significant negative correlation between T lymphocyte p56(lck) expression and serum Zn (r= -0.65, P = 0.0007) or femur Zn (r = -0.73, P = 0.0001) concentrations. We propose that elevated T lymphocyte p56(lck) may contribute to altered thymoctye maturation, apoptosis and lymphopenia in Zn deficiency and protein-energy malnutrition syndromes.  (+info)

Intracellular trafficking pathways in the assembly of connexins into gap junctions. (8/3761)

Trafficking pathways underlying the assembly of connexins into gap junctions were examined using living COS-7 cells expressing a range of connexin-aequorin (Cx-Aeq) chimeras. By measuring the chemiluminescence of the aequorin fusion partner, the translocation of oligomerized connexins from intracellular stores to the plasma membrane was shown to occur at different rates that depended on the connexin isoform. Treatment of COS-7 cells expressing Cx32-Aeq and Cx43-Aeq with brefeldin A inhibited the movement of these chimera to the plasma membrane by 84 +/- 4 and 88 +/- 4%, respectively. Nocodazole treatment of the cells expressing Cx32-Aeq and Cx43-Aeq produced 29 +/- 16 and 4 +/- 7% inhibition, respectively. In contrast, the transport of Cx26 to the plasma membrane, studied using a construct (Cx26/43T-Aeq) in which the short cytoplasmic carboxyl-terminal tail of Cx26 was replaced with the extended carboxyl terminus of Cx43, was inhibited 89 +/- 5% by nocodazole and was minimally affected by exposure of cells to brefeldin A (17 +/-11%). The transfer of Lucifer yellow across gap junctions between cells expressing wild-type Cx32, Cx43, and the corresponding Cx32-Aeq and Cx43-Aeq chimeras was reduced by nocodazole treatment and abolished by brefeldin A treatment. However, the extent of dye coupling between cells expressing wild-type Cx26 or the Cx26/43T-Aeq chimeras was not significantly affected by brefeldin A treatment, but after nocodazole treatment, transfer of dye to neighboring cells was greatly reduced. These contrasting effects of brefeldin A and nocodazole on the trafficking properties and intercellular dye transfer are interpreted to suggest that two pathways contribute to the routing of connexins to the gap junction.  (+info)

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TY - JOUR. T1 - High-Performance Immunoaffinity Chromatography and Chemiluminescent Detection in the Automation of a Parathyroid Hormone Sandwich Immunoassay. AU - Hage, David S.. AU - Kao, Pai C.. PY - 1991/3/1. Y1 - 1991/3/1. N2 - An automated sandwich immunoassay was developed based on high-performance immunoaffinity chromatography and chemiluminescent detection, using the determination of parathyroid hormone (PTH) in plasma as a model system. In this method, Injections of plasma and acrldinium ester-labeled antl-(1-34 PTH) antibodies were made onto a column containing Immobilized anti-(44-68 PTH) antibodies. Upon elution, PTH and Its associated labeled antibody were combined with an alkaline peroxide postcolumn reagent, and the resulting light production was measured. Factors considered in optimizing this system included the columns dissociation properties, the rate of light production in the postcolumn reactor, and the use of sequential vs simultaneous injection of sample and labeled ...
A monitor continuously senses light emitted by the chemiluminescent reaction of such substances as ozone and nitric oxide in order to measure an unknown quantity or concentration of one of the substances which may, for example, be an air pollutant. The monitor comprises a specially designed reaction chamber intended to utilize a high concentration reactant gas in such a fashion as to permit the measurement of light output of the chemiluminescent reaction with the sample gas by a solid state detector and associated electronics which eliminate the need for the use of a photomultiplier tube. The reactor body has an end closure means to form an annular plenum chamber surrounding a light transmitting means mounted by the closure means in one end of the reaction chamber. An annular discharge passage for discharging a conical curtain of reactant mixture from the plenum chamber axially into the reaction chamber around and away from the light transmitting means to prevent obscuration thereof by dirt from the
TY - JOUR. T1 - Flow and evaporation cells for the detection of proteins on membranes with the peroxyoxalate chemiluminescent reaction in organic media. AU - Castro-Hartmann, Pablo. AU - Daban, Joan Ramon. PY - 2004/8/1. Y1 - 2004/8/1. N2 - The high-energy intermediates generated in the reaction of bis(2,4,6-trichlorophenyl)oxalate (TCPO) with H2O2 can excite electronically different fluorophores with a high quantum yield in organic solvents. We have previously applied this peroxyoxalate chemiluminescent reaction to the detection of proteins labeled with the fluorescent dye 2-methoxy-2,4-dphenyl-3(2H)-furanone (MDPF) on polyvinylidene difluoride (PVDF) membranes. In this work, we have investigated the possibility to enhance the sensitivity of this detection method using specially designed cells in which the reagents TCPO and H2O2 in acetone are continuously renewed. In the flow cell, two syringes are used to renew the reagents in the reaction chamber containing the PVDF membrane with blotted ...
An optimized chemiluminescent assay for beta-galactosidase using a chemiluminescent substrate AMPGD (3-(4-methoxyspiro[1,2-dioxetane-3,2-tricyclo-[3.3.1. 1(3,7)]decan]-4- yl)phenyl-beta-D-galactopyranoside) is described. This assay is rapid and sensitive and can detect as little as 2 fg of beta-gal …
Neutrophil chemiluminescence in burned patients.: Phagocytic and bactericidal assays showed that a close correlation was present between bactericidal activity a
North America Chemiluminescence Immunoassays (CLIA) Market provides pictorial methodology along with detailed sizing by capturing all dependent markets
Vitamin B12 is very important for the human body. Early diagnosis of vitamin B12 deficiency is essential because it is associated with many problems such as fatigue, lethargy, depression, poor memory, breathlessness, headaches, pale skin, mania, psychosis, etc. Most of the vitamin B12 molecules in serum bind to specific proteins as a complex; so, although researchers have developed some sensitive methods to quantify vitamin B12, few can be used for human serum detection of vitamin B12 because of disturbed releasing process. In this study, we are aiming to develop a rapid and accurate chemiluminescence immunoassay (CLIA) for human serum testing ...
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In this research, the increase in sensitivity and decrease in diagnose limit of chemiluminescence system was made possible by producing gold/silver nanoalloy as the catalyst and introducing it in combination with Amplex red. Amplex red has unique luminescence properties (its oxidized form has fluorescence - chemiluminescence ability), and it is used as the probe. The observation of fluorescence caused by Amplex red in the absence of vitamin C a few minutes after the end of luminescence reaction confirms the presence of hydrogen peroxide as the oxidant in chemiluminescence system. In fact, hydrogen peroxide and its other active forms make possible the oxidation of Amplex red and its conversion into fluorescence form. Vitamin C, having antioxidant properties, is able to decompose hydrogen peroxide and disturb chemiluminescence reaction. Therefore, the designed luminescence system was used in the measurement of vitamin C in medical products ...
SeraCare is a trusted partner and worldwide supplier to the diagnostic testing industry, advancing the development of clinical genomics, molecular, and serological diagnostics. Check out how our product, LumiGLO Reserve® Chemiluminescent Substrate Kit 2400 cm2 can help with research, development, validation, and lab testing.
Hospital environment poses a substantial risk for transmission of pathogens. However, assessing cleaning efficiency is difficult and often is carried out by a subjective visual check only. The ATP-bioluminescence assay technology was developed to assist in evaluation of the environmental cleaning process by counting living and non-living organic matter in Relative Light Units (RLUs). In a 1000-bed university-affiliated hospital the standard practice following patient discharge includes terminal cleaning of the patient unit performed by nursing aides associated with the ward. ...
Assessment of ATP Bioluminescence monitoring in restaurants. Paul B. Allwood, PhD, MPH, RS Minnesota Department of Health St. Paul, Minnesota. How fireflies produce light. Luciferase/luciferin Oxygen ATP. Adenosine Triphosphate (ATP). Light proportional to ATP. Slideshow 3202236 by conley
Chemiluminescence immunoassay clia kits for the laboratory. Famous supplier of clia kits reagent,You can get the clia test kits from Elabscience.
Peri- and post-operative (up to day 7 after surgery) neutrophil chemiluminescence and the plasma concentrations of interleukin 6 (IL-6), IL-8, IL-10 and tumour necrosis factor alpha (TNF-alpha) were evaluated in the blood of patients undergoing liver transplantation. IL-6, IL-8 and IL-10 levels increased during early reperfusion and then returned to normal mostly within the first post-operative day. TNF-alpha was increased during the whole period observed. Spontaneous as well as activated neutrophil chemiluminescence was depressed in early reperfusion and remained low during the whole period followed. Samples collected during early reperfusion provided positive correlation for IL-6 vs IL-8 as well as for IL-6 and IL-8 vs chemiluminescence. The data were also evaluated with respect to the outcome of transplantation. Since IL-8, IL-10 and TNF-alpha levels increased significantly during the first post-operative week, mainly in a group of patients who developed serious complications within the first ...
297283835 - EP 0898620 B1 2002-11-06 - ASSAY OF PEROXIDASE ACTIVITY - [origin: WO9739142A1] Chemiluminescent detection of molecules of synthetic or natural origin such as proteins and nucleic acids (DNA and RNA), as well as other biologic molecules, is increasingly replacing radioactive detection as the method of choice where sensitivity is critical. In such assays, luminescence is customarily achieved by the oxidation of a luminol or isoluminol substrate in the presence of an oxidizing agent such as hydrogen peroxide or hydrogen peroxide source, such as perborate, and a peroxidase catalyst such as horseradish peroxidase. To obtain useful levels of luminescence (e.g., detectable levels) by customary techniques, a luminescent enhancer is also present during oxidation. It has been found in the practice of the present invention that azine enhancers have contained an impurity which reduces the properties of the chemiluminescent assay working solutions. The present invention describes a working solution, a
BioAssay record AID 632132 submitted by ChEMBL: Antagonist activity at rat MCHR1 expressed in HEK293 cells by aequorin bioluminescence assay.
Alibaba.com offers 155 luminometer products. About 27% of these are testing equipment, 25% are other measuring & analysing instruments, and 6% are other optics instruments. A wide variety of luminometer options are available to you,
In this article, is reported both cell and animal imaging onAlpha-synuclein (αSYN) aggregation, a pivotal role in the Parkinson disease
Recent advances in molecular biology have improved the detection applications of bioluminescence and chemiluminescence. These developments facilitate the continuous detection of biological processes in vitro and in vivo and can be applied to clinical, diagnostic and drug development. Moreover, hypersensitive and selective bioanalytical tools, such as recombinant cell biosensors, immunoassays and nucleic acid hybridization systems, have been developed by combining chemiluminescent enzyme assays or certain photoluminescent proteins with biologically specific binding sites at the gene level.. Since more than 20 years ago, Marlene DeLucas first successful access to the transgenic tobacco of firefly fluorometric enzyme assays, the application of bioluminescence and enzymology assays have entered a new era.. New BL / CL biotechnology tools. Reported gene technology represents one of the recent major advances in molecular biology. The reporter gene is a DNA sequence that encodes a protein or enzyme ...
Recent advances in molecular biology have improved the detection applications of bioluminescence and chemiluminescence. These developments facilitate the continuous detection of biological processes in vitro and in vivo and can be applied to clinical, diagnostic and drug development. Moreover, hypersensitive and selective bioanalytical tools, such as recombinant cell biosensors, immunoassays and nucleic acid hybridization systems, have been developed by combining chemiluminescent enzyme assays or certain photoluminescent proteins with biologically specific binding sites at the gene level.. Since more than 20 years ago, Marlene DeLucas first successful access to the transgenic tobacco of firefly fluorometric enzyme assays, the application of bioluminescence and enzymology assays have entered a new era.. New BL / CL biotechnology tools. Reported gene technology represents one of the recent major advances in molecular biology. The reporter gene is a DNA sequence that encodes a protein or enzyme ...
Minute WB Stripping Solution is designed to rapidly strip antibodies bound to antigens on Western blotting membranes using chemiluminescent substrate. One of the major advantages of chemiluminescent detection is high sensitivity and the ability to strip reagents from a blot and re-probe the same blot. Re-probing Western blots are very useful for optimizing the blotting conditions such as testing different antibodydilution and membrane blocking conditions. The key to this process is to useconditions that cause the release of antibody from the antigen without causing a significant amount of antigen to be released from the membrane. During the stripping procedure, small amount of antigen is inevitably lost from the membrane. It is important to minimize this loss by stripping the antibody under gentle conditions. Minute WB stripping solution is able to achieve maximum removal of antibodies from a membrane and preserving the integrity of the antigen in 10-15 min without using harsh conditions suchas ...
Peterson ME, DeMarco CL, Sheldon KM. in Scientific Proceedings (Abstract). American College of Veterinary Internal Medicine 2003;396. Endocrine testing of dogs
Peterson M.E., Demarco C.L. and Sheldon K.M. Conference Proceedings, (2003). American College of Veterinary Internal Medicine: p.396 Endocrine testing of dogs and cats is commonplace in clinical practice. Feline hyperthyroidism and canine hypothyroidism comprise the most frequently encountered endocrinopathies … Read More
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The effect of binding the Trp-free motor domain mutant of Dictyostelium discoideum, rabbit skeletal muscle myosin S1, and tropomyosin on the dynamics and conformation of actin filaments was characterized by an analysis of steady- state tryptophan phosphorescence spectra and phosphorescence decay kinetics over a temperature range of 140-293 K. The binding of the Trp-free motor domain mutant of D. discoideum to actin caused red shifts in the phosphorescence spectrum of two internal Trp residues of actin and affected the intrinsic lifetime of each emitter, decreasing by roughly twofold the short phosphorescence lifetime components (t 1 and t 2) and increasing by ;20% the longest component (t3). The alteration of actin phosphorescence by the motor protein suggests that i), structural changes occur deep down in the core of actin and that ii), subtle changes in conformation appear also on the surface but in regions distant from the motor domain binding site. When actin formed complexes with skeletal ...
Well-defined mechanistic principles have emerged to rationalize micellar enhancement of chemiluminescence. The review of Lin and Yamada[1] focuses on how micelles may be used to improve chemiluminescence signals by changes that affect the reaction rate. These occur in the microenvironment (i.e. polarity, viscosity and/or acidity, etc.), in the chemical and photophysical pathway and in the solubilization, concentration and organization of the solute/reactant. We shall now use these principles as a framework for discussing this work and it will become clear that they are highly inter-related rather than mutually exclusive.[2]. There follow examples of micellar enhancement which have been explained by changes in the microenvironment. In the interaction of sulfite groups in drugs with dissolved oxygen in presence of acidic rhodamine 6G, the surfactant Tween 60 can enhance chemiluminescence by 200%, attributable to a microenvironment that leads to an increase in the fluorescence quantum yield of ...
The chemiluminescent response of rabbit alveolar macrophages to foreign particles was investigated with regard to determination of the involvement of reactive forms of oxygen. The alveolar macrophages were obtained by tracheal lavage performed on New- Zealand-white-rabbits. The foreign particle used to initiate the chemiluminescent response was zymosan at a concentration of two milligrams per 10(7
Antibodies against insulin measured by electrochemiluminescence predicts insulitis severity and disease onset in non-obese diabetic mice and can distinguish human type 1 diabetes status. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
In the report the esoteric testing market, the market is expected to reach US$ $ 45,081.6 Mn in 2025 from US$ 19,868.2 in 2017. The market is estimated to grow with a CAGR of 11.7% from 2018-2025. The esoteric testing market is driven by the driving...
A method and apparatus for performing a chemiluminescent immunoassay featuring electrochemical techniques to generate an oxidant used to trigger the chemiluminescence of the labelled immunoreactant. The generation of the oxidant is precisely controlled and the oxidant precisely and uniformly delivered to provide a more accurate, precise and sensitive assay.
Product Description specificalThe micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CGalpha. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CGalpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CGalpha, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit(RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CGalpha. You can calculate the concentration of Human CGalpha in the samples by comparing the RLU value of the samples to the standard curve. ...
Bioluminescence methods are gaining increased attention due to their sensitivity, selectivity, and simplicity, along with the fact that bioluminescence can be monitored both in vitro and in vivo. This book introduces bioluminescence and fluorescence systems, along with the principles of their application for in vivo imaging of intracellular processes, and covers recent developments in optical (bioluminescence and fluorescence) imaging in cell biology. This book is intended for scientists and students involved in basic cell physiology research, as well as industry professionals, engineers, and managers involved in drug discovery and pre-clinical drug development. It discusses the practical aspects of luminescence in vivo imaging for monitoring intracellular processes. While some basic knowledge of biochemistry and biophysics is preferable, the book includes a brief review of fundamental principles to allow those not familiar with these disciplines to grasp basic concepts. ...
This complete and well-organized overview of chemiluminescence and bioluminescence is divided into two parts. The first covers historical developments and the fundamental principles of these phenomena before going on to review recent advances and instrumentation. The second part deals with the applications in a variety of research fields including life sciences, drug discovery, diagnostics, environment, agrofood, and forensics. The book is suitable not only for researchers currently employing detection techniques in their research activity, but also for those approaching the subject for the first time. Particular emphasis is placed on the use of chemiluminescence and bioluminescence for the development of a variety of (bio)analytical methods, such as flow-assisted methods, enzyme-, antibody- or gene probe-based assays also in multiplexed formats, miniaturized analytical devices, biosensors, BRET and protein complementation assays, whole-cell biosensors, and bioluminescence molecular imaging. Individual
Herein, an ultrasensitive and stereo-selective electrochemiluminescent (ECL) biosensor based on ECL signal amplification of luminol by the synergetic catalysis of a hemin-functionalized composite and gold-platinum nanowires (Au-PtNWs) has been designed for the detection of d-alanine (d-Ala). In the sensor de
TY - JOUR. T1 - Clinical performance of the Elecsys electrochemiluminescent immunoassay for the detection of SARS-CoV-2 total antibodies. AU - Favresse, Julien. AU - Eucher, Christine. AU - Elsen, Marc. AU - Marie, Tré-Hardy. AU - Dogné, Jean-Michel. AU - Douxfils, Jonathan. PY - 2020/8/1. Y1 - 2020/8/1. KW - COVID-19. KW - SARS-CoV-2. KW - evaluation. KW - serology. KW - kinetics. UR - https://researchportal.unamur.be/en/publications/clinical-performance-of-the-elecsys-electrochemiluminescent-immunoassay-for-the-detection-of-sarscov2-total-antibodies(16ab5056-361a-49db-b30a-c0259d072208).html. UR - http://www.scopus.com/inward/record.url?scp=85087489211&partnerID=8YFLogxK. U2 - 10.1093/clinchem/hvaa131. DO - 10.1093/clinchem/hvaa131. M3 - Letter. C2 - 32484887. VL - 66. SP - 1104. EP - 1106. JO - Clinical Chemistry. JF - Clinical Chemistry. SN - 0009-9147. IS - 8. M1 - hvaa131. ER - ...
Osteocalcin by Electrochemiluminescent Immunoassay,ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative laboratory research and development. ARUP offers an extensive test menu of highly complex and unique medical tests in clinical and anatomic pathology. Owned by the University of Utah, ARUP Laboratories client,medicine,medical supply,medical supplies,medical product
LOC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET NUCLEIC ACID SEQUENCES WITH CALIBRATED PHOTODETECTION OF PROBES IN HYBRIDIZATION ARRAY - diagram, schematic, and image 103 ...
LOC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET NUCLEIC ACID SEQUENCES WITH CALIBRATED PHOTODETECTION OF PROBES IN HYBRIDIZATION ARRAY - diagram, schematic, and image 84 ...
The wavelengths of chemiluminescence emission that are analytically useful depend on the characteristics of the detector. Visible emission (though it is seldom visible to the naked eye) has a wavelength range of about 400-750 nm, corresponding to enthalpy changes of exothermic reactions of between 180 and 300 kJ mol−1, provided that there is a pathway to an excited state that relaxes with the loss of a photon (see Figure 1.1). Emission intensity is proportional to the concentration of the emitting species, which is either an intermediate or a product in an electronically excited state. This concentration depends on the rate of the reaction producing it. Analytical detection of chemiluminescence usually involves no wavelength selection, i.e., it is emission photometry rather than emission spectrophotometry. Selectivity is achieved by on-line treatments rather than by processing of the signal, which has little fine-structure.[1]. Because of this, the importance of chemiluminescence spectroscopy ...
Bioluminescent Reporter plasmid to express bacterial luciferase (Lux) in Mycobacteria Background. Bioluminescence, the production of light by luciferase-catalysed reactions, is a versatile reporter technology with multiple applications both in vitro and in vivo. In vivo bioluminescence imaging (BLI) represents one of its most outstanding uses by allowing the non-invasive localisation of luciferase-expressing cells within an animal. Applied to the study of infectious diseases, BLI permits the detection of microorganisms from within living animals thus allowing the spatiotemporal study of infection in real-time in the same host. Moreover, using luciferase as a reporter of gene expression, it is possible to establish when and where a gene function is needed, shedding light on bacterial pathogenesis. Finally, BLI constitutes an easy and rapid method to test novel antimicrobial compounds in vivo.. Three main luciferin-luciferase systems have been utilised for BLI. The first system is represented by ...
Sensitive and feasible electrochemiluminescence (ECL) bioassay relies on the achievement of the ECL signal tags with high and stable signal intensity. In this work, strong electrochemiluminescence (ECL) emission was achieved from metal organic framework (MOFs) accelerator enriched Quantum Dots (CdTe), which was applied as an efficient ECL signal tag for trace biomarker detection. It is particularly worth noting that a novel mechanism to drastically enhance the ECL intensity of CdTe is established because the isoreticular metal organic framework-3 (IRMOF-3) with 2-amino terephthalic acid (2-NH2-BDC) as organic ligand is not only devoted for loading large amount of CdTe via encapsulating effect and internal/external decoration, but also as a novel co-reactant accelerator for promoting the conversion of co-reactant S2O82- into the sulfate radical anion (SO4•-), further boosting the electrochemiluminescence (ECL) emission of CdTe ...
CTX : Testing is performed using the Roche Cobas 6000 e601 analyzer and the Roche Beta-CrossLaps assay, a 2-site immunometric (sandwich) assay using electrochemiluminescence detection. Patient specimen, biotinylated monoclonal beta-CrossLaps-specific antibody, and monoclonal beta-CrossLaps-specific antibody labeled with ruthenium react to form a complex. Streptavidin-coated microparticles act as the solid phase to which the complex binds. Voltage is applied to the electrode, inducing a chemiluminescent emission from the ruthenium, which is then measured against a calibration curve to determine the amount of beta-CrossLaps in the patient specimen. This assay is specific for crosslinked isomerized type I collagen fragments, independent of the nature of the crosslink (eg, pyrrole, pyridinolines). The assay specificity is guaranteed through the use of 2 monoclonal antibodies, each recognizing linear beta-8AA octapeptides (EKAHD-beta-GGR). The assay, therefore, quantifies all type I collagen degradation
OBJECTIVE: Activated phagocytes, generating a variety of powerful inflammatory mediators, such as oxygen and nitrogen species, may participate in oxidative stress-mediated inflammation and organ toxicity. At present, great attention is devoted to the important class of phenolic compounds - coumarins - due to their antiinflammatory/antioxidant activities. We compared two synthetic phenylcoumarins: 7-hydroxy-3- (4-hydroxyphenyl) coumarin (HHC; 0.01-100 mu mol/l) and its hydrogenated analogue: 7-hydroxy-3-(4-hydroxyphenyl)-3,4-dihydrocoumarin (HHDC; 0.01-100 mu mol/l) as their ability to inhibit reactive oxygen species (ROS) generation in human neutrophils and nitric oxide (NO) production by RAW 264.7 macrophages in vitro, with respect to some of their physicochemical characteristics. METHODS: ROS production was measured with luminol-enhanced chemiluminescence (CL) in the microplate luminometer Immunotech LM-01T, nitrite formation was determined by the Griess reaction - spectrophotometrically. ...
Publications:. Stevani, C.V., Silva, S.M. & Baader, W.J. Studies on the Involvement of the CIEEL Sequence in the Peroxyoxalate Reaction; em: Bioluminescence and Chemiluminescence, Perspectives for the 21st Century, eds: Roda, A., Pazzagli, M., Kricka, L.J. & Stanley, P.E., John Wiley & Sons, Bologna, Italy, 33-38, 1999.. Stevani. C.V, Silva, S.M. & Baader, W.J Studies on the Mechanism of the Excitation Step in Peroxyoxalate Chemiluminescence Eur. J. Org. Chem., 2000, 4037-4046.. Silva, S.M., Wagner, K., Weiss, D., Beckert, R. & Baader, W.J. Studies on the Chemiexcitation Step in Peroxyoxalate Chemiluminescence Using Steroid-Substituted Activators Luminescence, 2002, 17, 362-369.. Silva, S.M., Jr., F.C., Oyamaguchi, K.H., Ciscato, L.F.L.M., Stevani, C.V. & Baader, W.J. Kinetic Studies on the Peroxyoxalate Chemiluminescence Reaction: Determination Of the Cyclization Rate Constant Luminescence, 2002, 17, 313-320.. Martins TS, Araújo AAS, Silva SM, Matos JR, Isolani PC & Vicentini G, ...
The temporal effects of luciferase reaction luminescence have only been discussed in the context of light intensity (flash vs. glow). However, alterations in the color of the light emitted over the course of the luciferase reaction have not been reported. Here, we show a temporal change in the light color emitted during the reaction catalyzed by unmodified firefly luciferase when concentrations of one of the substrates, adenosine triphosphate (ATP), are gradually increased. The temporal color change from green to red occurs within the first few minutes of the luciferase reaction when an ATP-containing solution is either added or synthesized in situ with the aid of an autocatalytic reaction occurring simultaneously ...
Promega offers several reporter and assay combinations for different situations.This article helps you choose the bioluminescent reporter protein and assay chemistry that is right for your needs.
Read independent reviews on Orion L Microplate Luminometer from Titertek-Berthold (Berthold Detection Systems GmbH) on SelectScience
The possibility that the mode of action of sulphasalazine and its active metabolite 5-amino-salicylic acid (5ASA) involves modification of toxic oxygen metabolite production by neutrophils has been investigated by measuring the effect of these drugs on luminol-dependent chemiluminescence, superoxide release and oxygen consumption by stimulated neutrophils in vitro. 5ASA, and to a lesser extent sulphasalazine, had profound inhibitory effects on the luminol dependent chemiluminescent response of neutrophils stimulated with formyl-methionyl-leucyl-phenylalanine (1 microM) + cytochalasin B (5 micrograms/ml). A concentration of 50 microM 5ASA or sulphasalazine produced 93.8 (2.3)% and 65.7 (3.7)% inhibition of control responses respectively. The concentration of 5ASA and sulphasalazine producing 50% inhibition of chemiluminescence were 3.6 (1.8) microM and 16.5 (6) microM respectively. Both drugs had little effect on the chemiluminescent response of neutrophils stimulated with phorbol myristate ...
TY - JOUR. T1 - Real-time monitoring of bioaerosols via cell-lysis by air ion and ATP bioluminescence detection. AU - Park, Chul Woo. AU - Park, Ji Woon. AU - Lee, Sung Hwa. AU - Hwang, Jungho. PY - 2014/2/15. Y1 - 2014/2/15. N2 - In this study, we introduce a methodology for disrupting cell membranes with air ions coupled with ATP bioluminescence detection for real-time monitoring of bioaerosol concentrations. A carbon fiber ionizer was used to extract ATP from bacterial cells for generating ATP bioluminescence. Our methodology was tested using Staphylococcus epidermidis and Escherichia coli, which were aerosolized with an atomizer, and then indoor bioaerosols were also used for testing the methodology. Bioaerosol concentrations were estimated without culturing which requires several days for colony formation. Correlation equations were obtained for results acquired using our methodology (Relative Luminescent Unit (RLU)/m3) and a culture-based (Colony Forming Unit (CFU)/m3) method; ...
Isolated equine alveolar macrophages were shown to generate a luminol-dependent light response when challenged with a phagocytic stimulus. The chemiluminescent response was not detected with luminol prepared at 1.0 x 10(-5) or 1.0 x 10(-4) molar concentrations, but was readily quantitated when used at a 1.0 x 10(-3) molar concentration. Challenge of the alveolar macrophages with latex particles or with equine IgG-coated sheep red blood cells elicited the luminol-dependent light response, whereas unchallenged equine alveolar macrophages or those challenged with unopsonized erythrocytes failed to emit light above background levels. Latex-bead-challenged macrophages released 8.06 times the total amount of light as those equine alveolar macrophages challenged with equine IgG-opsonized erythrocytes. This study represents the first investigation on chemiluminescence and equine alveolar macrophages. ...
The authors reported on the cervical histologic results of 209 patients demonstrating equivocal results for hrHPV from SurePath (204 patients) or ThinPrep (5 patients) vials, and compared patients in this cohort with atypical squamous cells of undetermined significance (ASC-US) cytology on the index cervical Papanicolaou (Pap) test (Group 1; n = 148 patients) with a patient cohort demonstrating unequivocal positive hrHPV test results (Group 2; n = 148 patients). The chemiluminescence intensity of hrHPV tests from patients in Group 2 were correlated with the presence and severity of dysplasia on subsequent histologic results, and patients were thereby stratified for their subsequent risk of cervical intraepithelial neoplasia (CIN) types II/III. ...
Title: Recent Applications of Chemiluminescence Assays in Clinical Immunology. VOLUME: 10 ISSUE: 14. Author(s):A. R. Rodriguez-Orozco, H. Ruiz-Reyes and N. Medina-Serriteno. Affiliation:Posgraduate Division, Medical College Dr. Ignacio Chavez, University of San Nicolas De Hidalgo, Rafael Carrillo esquina Salvador Gonzalez Herrejon s/n. Bosque Cuauhtemoc, Colonia Centro, CP 58000, PO Box 136, Morelia, Michoacan, Mexico.. Keywords:Chemiluminescence assays, phagocytic activity, clinical immunology, tumors, immunodeficiencys states, inflammatory response, infectious diseases, endocrine disorders, cell activation, adjuvants, cytotoxic drugs, autoimmune diseases, inflammatory responses, immunodeficiencys states, electromagnetic radiation, neutrophils, PMN, monocytes, MN, NADPH oxidase, Luminol, singlet oxygen, superoxide anion, hydrogen peroxide, hypochlorous acid, IL-1 receptor antagonist, Nitric Oxide (NO)/peroxynitrite formation, polymorphonuclear leukocytes, rheumatism, PCR/RT-PCR techniques, ...
While lateral flow test formats can be utilized with whole blood and low sample volumes, their diagnostic characteristics are inferior to immunoassays based on chemiluminescence immunoassay (CLIA) or enzyme-linked immunosorbent assay (ELISA) technology. CLIAs and ELISAs can be automated to a high degree but commonly require larger serum or plasma volumes for sample processing. We addressed the suitability of EDTA-anticoagulated whole blood as an alternative sample material for antibody testing against SARS-CoV-2 by electro-CLIA (ECLIA; Roche, Rotkreuz, Switzerland) and ELISA (IgG and IgA; Euroimmun, Germany). Simultaneously drawn venous serum and EDTA-anticoagulated whole blood samples from 223 individuals were included. Correction of the whole blood results for hematocrit led to a good agreement with the serum results for weakly to moderately positive antibody signals. In receiver-operating characteristic curve analysis, all three assays displayed comparable diagnostic accuracy (area under the ...
A new flow-injection chemiluminescence method for the determination of iron is described. It is based upon the chemiluminescence reaction of iron (Ⅱ)-lucigenin system with Jones reductor. The chemiluminescence signal-to-noise ration can be improved apparently by the addition of cetyltrimethyl- ammonium bromide and emulsor OP. The present method has a wide linear of 1×10-11~1×10-5 g/mL for Fe(Ⅱ). The detection limit is 2 × 10-12 g/mL. The relative standard deviation is 2.0% (1× 10-8 g/mL Fe, n = 11). The method has been applied to the determination of iron in hair samples with satisfactory results. The mechanism of this reaction is also investigated in detail.
The statistical surveying report on the global Automatic Chemiluminescence Immunoassay Analyzer Market has been presented by using professional or expert knowledge through standard and modified...
Farm collaborated with BioKit to help launch its first proprietary clinical analysis device, a chemiluminescent immunoassay analyzer designed to establish and enhance BioKits brand identity.
Read independent reviews on cAMP-Screen ® and cAMP-Screen Direct ® Chemiluminescent Immunoassay System for Determination of cAMP Concentration from Thermo Fisher Scientific on SelectScience
TY - JOUR. T1 - Solvent-Slaved Dynamic Processes Observed by Tryptophan Phosphorescence of Human Serum Albumin. AU - Draganski, Andrew R.. AU - Friedman, Joel M.. AU - Ludescher, Richard D.. PY - 2017/3/14. Y1 - 2017/3/14. N2 - Despite extensive experimental and computational efforts to understand the nature of the hierarchy of protein fluctuations and the modulating role of the protein hydration shell, a detailed microscopic description of the dynamics of the protein-solvent system has yet to be achieved. By using single tryptophan protein phosphorescence, we follow site-specific internal protein dynamics over a broad temperature range and demonstrate three independent dynamic processes. Process I is seen at temperatures below the bulk solvent Tg, has low activation energy, and is likely due to fast vibrations that may be enabled by water mobility on the protein surface. Process II is observed above 170 K, with activation energy typical of β relaxations in a glass; it has the same temperature ...
Figure D11.1 - On-column coaxial flow interface between capillary electrophoresis and chemiluminescence detection (adapted from reference D9.1).. As with HPLC, there is an inherent problem of compatibility between the conditions needed for separation and those needed for chemiluminescence. Additionally, there is a potential problem with the stability of chemiluminescence reagents. Both of these are addressed by using the post-column mode rather than pre-column. Post-column interfaces are devices for mixing the eluent with the chemiluminescence reagents and for this purpose designs may make use of merging flow, coaxial flow or reservoir mixing. Interfaces may also be classified as off-, on- or end-column, depending on the site of detection and on whether this is isolated from the high voltage supply used for capillary electrophoresis.. The simplest interface, off-column and merging flow, did not find widespread application. Buffer flowed from a reservoir through the separation capillary and ...
The unique cathodic electrochemiluminescence(ECL) emission of Ru(bpy)32+(bpy=2,2′-bipyridine) was observed via Nafion film at Au electrode[Au/Nafion/Ru(bpy)32+] at about 0.20 V(vs. Ag/AgCl) and applied to the determination of several amino acids without prior derivatization with high sensitivity. The cathodic electrochemiluminescence(ECL) exhibits the detection limits and linear ranges of several amino acids comparable to or better than those of capillary electrophoresis with conventional ECL detection method(at 1.10-1.20 V vs. Ag/AgCl) based on precolumn derivatization. The results suggest that the cathodic ECL is promising for the detection of amino acids in bioanalysis.. ...
Аннотация: Two methods for phenol determination based on the suppression of chemiluminescence (CL) produced upon HRP-catalysed oxidation of luminol were developed. One method used an enhanced CL reaction with 3-(100-phenothiazinyl)-propane-1-sulfonate and 4-morpholinopyridine as primary and secondary enhancers, respectively, and another one is free of the enhancers. A comparison of the phenol assay with enhancers showed much higher sensitivity and lower LDL value than the assay without enhancers. In addition, the amount of HRP used in the assay with enhancers is 40 times less than in the assay without them. The study of the specificity of the developed assays demonstrated that in the assay without enhancers several phenol compounds, for instance, unsubstituted phenol, suppressed the CL intensity, whereas other phenols, such as 4-chlorophenol and 2,4-dichlorophenol, increased the light output. In contrast to that assay, in the assay with enhancers all tested phenols suppressed the CL ...
Continuous increase in the number and the variety of anthropogenic sources of electromagnetic radiation causes a high interest in studying the effects ultrahigh frequency on living organisms. In the present research inflence of UHF EMR (15 W, 2.45 GHz) for 5 and 15 min on morphological and genetic peculiarities of Photobacterium phosphoreum colonies was studied. It has been revealed that UHF EMR affected colony growth parameters, induced transcriptional activity of luciferase encoding gene expression and that the effect was depended on exposure duration. The subsequent cultivation of bacteria during a two week period after treatment showed maintaining of the increased luxb mRNA level in irradiated colonies. Opposite bacterial stress responses were detected to UHF EMR and elevated temperature treatments that assumed UHF EMR comprised of not only thermal but specifi component of non-thermal nature.. The Opened International University of Human Development Ukraine ...
The validity of chemiluminescence-based methods relies upon the uniqueness of the relationship between the concentration of the analyte and the intensity of chemiluminescence produced. We have examined the chemiluminescence of luminol (5-amino-2,3-dihydro-1,4-phthalazinedione) in the presence of O2, without added H2O2, to measure nanomolar concentrations of total Fe(II) (both inorganically and organically complexed) in aqueous samples. To test the validity of the method, we have developed a kinetic model that describes the two-step oxidation of luminol by superoxide and hydroxyl (or hydroxyl-like) radicals produced from the oxidation of Fe(II) by O2 by synthesis of existing published data. This model was coupled with a model for Fe(II) oxidation by O2 in the presence and absence of the naturally occurring Suwannee River fulvic acid (SRFA). The production of chemiluminescence depended upon the concentrations of free radicals in both the sample and luminol reagent and the pH at which the reactions were
Presence of anti-HCV antibodies indicates prior exposure to HCV infection. However, the assay may be negative early in acute HCV infection, in immunosuppressed individuals. Anti-HCV antibodies can be detected by rapid immunoassays, enzyme-linked immunosorbent assays (ELISA), or chemiluminescent immunoassays. Quality of some of the test kits for anti-HCV may be a reason for concern.. Rapid immunoassay tests use a unique combination of modified HCV antigens conserved across all genotypes. However, some studies have demonstrated that rapid tests cannot be solely relied upon in screening patients from high- risk population in India. ELISA tests indicate presence of antibodies against HCV core, NS3 and NS5 regions. A study in North India has demonstrated the value of blood banks screening using two ELISA kits concurrently to improve detection of contaminated blood.. Enhanced chemiluminescence immunoassay (ECi) a quantitative assay based on chemiluminescent reaction, which is as good as ELISA.. ...
Detectable compounds comprising a chemically-transformable first compound covalently linked to an electrochemiluminescent compound are provided. Such compounds are useful in processes and kits that monitor the status of the first compound and derive information from such monitoring. A rapid single step assay suitable for the detection or quantification of β-lactam antibiotics and β-lactamases. The assay can be performed directly on samples of food, such as milk and meat, blood or serum and is useful in determining the suitability of a particular antibiotic in treating a particular bacterial infection and in diagnosis of a bacterial infection. The assay is also useful in determining and quantifying β-lactam antibiotic resistance. The assay can be performed on an IGEN OrigenR Analyzer.
If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Centers RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.. ...
The limit of detection calculated from repeated measurements of the zero calibrator was found to be ,0.6 kilounits/L in all analyses. This is equivalent to the readable limit of the analyzer. One laboratory performed measurements in a serum sample with a CA 125 value of 0.8 kilounits/L. The limit of detection obtained for serum was 1.1 kilounits/L, which is highly sufficient for diagnostic purposes.. According to the manufacturer, the assay is linear up to 5000 kilounits/L. This was checked in four laboratories in a total of 24 dilution series covering three decades of CA 125 concentrations, using either human sera or diluent for dilution. On the basis of an acceptance range of 90-110% recovery, the linearity of the assay when serum was used as diluent was confirmed throughout the range studied (highest serum concentration ∼4500 kilounits/L) with the exception of the highest dilution, which exceeded the acceptance criteria in 5 of 12 experiments. The acceptance criteria were not met with the ...
Virus-like particles (VLPs) offer a system to test the hypothesis that since antibody binding to native cover glycoprotein (Env) trimers ends up in HIV-1 neutralization that local Env trimers presented in membranes can be useful for causing neutralizing antibodies (nAbs) within a vaccine placing. models. The latest immunogenicity research in rabbits appear to have out all of the 3 for these leads. plus the Animal Wellbeing Act. Macaques were encased indoors in stainless steel galetas had a 12h/12h daily lumination cycle had been fed 2 times daily given water R595 cell wall membrane skeleton out of and man made trehalose dicorynomycolate. Adjuplex is made up of detergent-free carbomer and lecithin homopolymer. AS01B consists of liposomes containing deacylated QS21 and MPL. Normally immunogens and adjuvants had been co-formulated. In buy 1207456-01-6 some cases had been administered independently (Fig. 2). iv) Medication dosage Gp120 and gp41 had been administered in 100μg amounts. Doses ...
A novel dioxetane compound is provided having a cationically polymerizable oxetane group, which compound is excellent in compatibility with a liquid crystalline compound and a non-liquid crystalline compound. An optical film is also provided with excellent liquid crystal orientation retention properties and mechanical strength, produced by aligning a composition of the dioxetane compound and a cationically polymerizable compound in a liquid crystal orientation and fixing the liquid crystal orientation by polymerization. Further, a liquid crystal display device is provided with the optical film.
Read A novel ultrasensitive electrochemiluminescence biosensor for glutathione detection based on poly-L-lysine as co-reactant and graphene-based poly(luminol/aniline) as nanoprobes, Biosensors and Bioelectronics on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
CURRENT LUMINESCENCE BIOASSAYS RELY SOLELY UPON MESUREMENT OF LUMINESCENCE INTENSITY TO GENERATE INFORMATION. MULTIPLE COLORS FROM BEETLE LUCIFERASE HAS CREATED THE OPPORTUNITY TO INCREASE ASSAY INFORMATION CONTENT BY USING MULTIPLEXING TECHNIQUES TO MEASURE LIGHT INTENSITIES AT DIFFERENT WAVELENGTHS. THIS PROVIDES GREATER ASSAY RELIABILITY, PRECISION, AND ABILITY TO MEASURE CONCURRENT MOLECULAR EVENTS. THE EXCELLENT PERFORMANCE OF BEETLE LUCIFERASE ENZYMES IN BIOASSAY METHODS YIELDS THE HIGHEST QUANUM EFFICIENCY OF ANY LUMINESCENCE METHODOLOGY, AND DEMONSTRATES SUPERIOR PRECISION, LINEARITY, AND RAPIDITY. TO FURTHER ASSESS THE FEASIBILITY OF USING MULTIPLEXING TO INCRASE THE COMMERCIAL POTENTIAL OF BIOLUMINESCENCE ASSAYS, RESEARCHERS ARE IDENTIFYING NOVEL SUBSTITUTIONS THAT REGULATE COLOR BY EXAMINING THE RELATIONSHIP BETWEEN AMINO ACID SUBSTITUTIONS IN THE PRIMARY STRUCTURES OF BETLE LUCIFERASES TO THE ENZYMES SPECTRAL PROPERTISE ...
This work demonstrates an aptasensor for ultrasensitive electrochemiluminescence (ECL) detection of thrombin based on an off-on-off approach. The system is composed of an Eu3+-doped CdS nanocrystals (CdS:Eu NCs) film on glassy carbon electrode (GCE) as ECL emitter. Then gold nanoparticles (AuNPs) labeled hairpin-DNA probe (ssDNA1) containing thrombin-binding aptamer (TBA) sequence was linked on the NCs film, which led to ECL quenching (off) as a result of Förster-resonance energy transfer (FRET) between the CdS:Eu NC film and the proximal AuNPs. Upon the occurrence of hybridization with its complementary DNA (ssDNA2), an ECL enhancement (on) occurred owing to the interactions of the excited CdS:Eu NCs with ECL-induced surface plasmon resonance (SPR) in AuNPs at large separation. Thrombin could induce ssDNA1 forming a G-quadruplex and cause the AuNPs to be close to CdS:Eu NCs film again, which resulted in an enhanced ECL quenching (off). This off-on-off system showed a maximum 7.4-fold ...
Lucigenin-enhanced chemiluminescence, reflective of testis superoxide levels. Luminescence was recorded in the absence or presence of NADPH (100 µmol/l). C: a
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
The bacterial luciferase gene cassette (lux) is unique among bioluminescent bioreporter systems due to its ability to synthesize and/or scavenge all of the substrate compounds required for its production of light. As a result, the lux system has the unique ability to autonomously produce a luminescent signal, either continuously or in response to the presence of a specific trigger, across a wide array of organismal hosts. While originally employed extensively as a bacterial bioreporter system for the detection of specific chemical signals in environmental samples, the use of lux as a bioreporter technology has continuously expanded over the last 30 years to include expression in eukaryotic cells such as Saccharomyces cerevisiae and even human cell lines as well. Under these conditions, the lux system has been developed for use as a biomedical detection tool for toxicity screening and visualization of tumors in small animal models. As the technologies for lux signal detection continue to improve, it is
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TY - JOUR. T1 - Correlation among monitoring methods of surface cleaning and disinfection in outpatient facilities. AU - Furlan, Mara Cristina Ribeiro. AU - Ferreira, Adriano Menis. AU - Rigotti, Marcelo Alessandro AU - Guerra, Odanir Garcia. AU - Frota, Oleci Pereira. AU - Sousa, Álvaro Francisco Lopes de. AU - C. de Andrade, Denise PY - 2019. Y1 - 2019. N2 - Objectives: To evaluate the correlation among microbiological culture, ATP bioluminescence assay, and visual inspection in monitoring the effectiveness of surface cleaning and disinfection in an outpatient facility and determine the ATP bioluminescence cutoff capable of indicating a clean surface regarding microbiological evaluation. Methods: Exploratory, cross-sectional, and correlation study consisting of 720 evaluations in five surfaces before and after cleaning and disinfection. The results were used to run two-proportions tests, calculate Spearmans correlation, and plot the receiver operating characteristic curve. Results: Similar ...
Manufactured by Caliper Life Sciences. The IVIS 100 Imaging System is a highly sensitive, low light-level system optimized for in-vivo (whole, living animal) imaging. The system is composed of: 1) 1-inch ultra-sensitive CCD camera cooled with a closed cycle mechanical refrigeration unit to -105°C; 2) light-tight imaging chamber; 3) a PC computer used to operate the IVIS, collect and store images, and image analysis; 4) the Living Image® software that runs the IVIS System. The System takes very low-light level images, stores them, and displays them for subsequent analysis.. Operation of the IVIS Imaging System is simple and straightforward. Place the anesthetized animal or animals on the shelf in the imaging chamber and close the door. Collect a preliminary image for animal positioning and if necessary, adjust the field of view and focus with the AFOV and AFocus controls, in the Camera Control panel. Click Acquire in the Camera Control panel, and the system will take a picture per your settings ...
The Difference Between Fluorescence & Phosphorescence: What is Fluorescence? Some materials absorb invisible, high-energy black (ultraviolet) light, use part of the energy, then emit nanoseconds later bright visible light, but only while the ultraviolet light shines on it. What is Phosphorescence? Phosphorescence is similar to fluorescence. Both involve absorption of energy. Glow-in-the-dark light (phosphorescence) is not as bright as fluorescence but lasts much longer. Phosphorescence is controlled by forbidden quantum physics transitions. Quantum Physics Transitions Quantum physics transitions are changes in molecular states made possible by energy absorption. Energy changes are quantized if they can only be absorbed or emitted in predetermined values or quanta. After absorption, lower energy photons (light particles) can be re-emitted. Practical Uses Fluorescence is utilized in medicine, while phosphorescence is used for glow-in-the-dark products. Hobbyist Uses Mineralogists treasure ...
China Cardiovascular series catalog of Myoglobin (Myo) Kits (Chemiluminescence Immunoassay), HS-Ctni Reagent (chemiluminescence assay) Heart Failure Diagnosis provided by China manufacturer - Nanjing Norman Biological Technology Co., Ltd., page1.
A method for the rapid and sensitive determination of organophosphate and carbamate pesticides is disclosed. The method employs an insect brain preparation having a mixture of receptors or enzymes wit
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Uruguay has a flock of nine six-month brilliant lambs which behave as any other sheep but are really genetically modified and are planned to help with medicine research. They were born in a farm belonging to the Animal Reproduction Institute of Uruguay (Irauy) a non profit organization connected to the Genetically Modified Animals Unit from the Pasteur Institute, a branch in Montevideo of the renowned French scientific organization.
It is not surprising, then, that many measures of disease burden concentrate on the factors that cause illness or make it worse. Wound-related bacteria can release metalloproteases and other mediators of inflammation that will lead to local tissue damage. Wound Management Challenges in Prison Populations, Hard-to-Heal Wounds: Steps for Interventions, Strategies for Early Biofilm Interventions, Exponential Benefits: Positive Patient Impacts with the Standardized Use of Hypochlorous Acid, Investigating the benefits of placental tissue in the wound healing process, A New Approach to Managing Wound Exudate During Challenging Times, Biofilm Management Using a Wound Hygiene Protocol, Protecting the skin microbiome and preventing pressure injuries with Cardinal Health™ breathable adult briefs, Arterial Ulcers: Assessment and Treatment. Medium 8Look฀for฀the฀signs฀outlined฀in฀the฀enabler฀ NERDS. Other bacteria stain red. The ATP bioluminescence method has been increasingly employed as a ...
GloMax Navigator is an easy-to-use plate-reading luminometer integrated with Promega chemistries for superior bioluminescence detection.
The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and time-resolved fluorescence (50-1450 µsec, 200 µsec increments) measurements in 6, 12, 24, 48, 96, and 384 well microplates or in a cuvette.. ...
For a laboratory that needs hassle-free chemiluminescent detection, as well as routine gel documentation, using the G:BOX Chemi XRQs powerful GeneSys software to switch between applications is simplicity itself. Place your chemi blot in, and the systems cooled camera captures images with a sensitivity equal to film or slide in your gels to generate quality pictures of your DNA and proteins anytime you want.. Featuring a standard darkroom and a 4 million pixel image resolution, the G:BOX Chemi XRQ camera is cooled to provide a low signal to noise ratio with perfect background.. HI-LED lighting options cover the full spectrum of high intensity blue, green, red and infra-red resulting in faster exposure times and publication quality images.. The G:BOX Chemi XRQ is a cost-effective alternative to laser based technology offering a faster workflow for imaging complex multiplex fluorescent gels and blots.. The system is controlled by GeneSys application driven image capture software and comes ...
Publications:. Rowe L, Teasley K, Dikici E, Qu X, Ensor M, Deo S, Daunert S. Recombinant Aequorin-Based Systems for Biomarker Analysis. Editor(s): Marks, Robert S. Handbook of Biosensors and Biochips, John Wiley & Sons, 1:173-186, 2007.. Teasley Hamorsky K, Ensor CM, Wei Y, Daunert S. A Bioluminescent Molecular Switch for Glucose. Angewandte Chemie, International Edition 47(20): 3718-3721, 2008.. Teasley Hamorsky K, Dikici E, Ensor CM, Daunert S. Biotechnological Improvements of Bioluminescent Systems. Editor(s): Roda, Aldo. Analytical Chemiluminescence and Bioluminescence: past, present and future, Royal Society of Chemistry, 1:443-487, 2010.. Scott D, Teasley Hamorsky K, Ensor CM, Anderson K, Daunert S. Cyclic AMP Receptor Protein-Aequorin Molecular Switch for Cyclic AMP. Bioconjugate Chemistry 22(3): 475-481, 2011.. Teasley Hamorsky K, Ensor CM, Pasini P, Daunert S. A Protein Switch Sensing System for the Quantification of Sulfate. Analytical Biochemistry 421(1): 172-180, 2012.. Teasley ...
BioTek Wissenschaftliche Poster, 29-Apr-09, Automation of Luminescence-based CYP Inhibition Assays using BioTek Instrumentation for use in Drug Discovery
Increased oxidative stress plays a significant role in the etiology of bone diseases. Heightened levels of H2O2 disrupt bone homeostasis, leading to greater bone resorption than bone formation. Organochalcogen compounds could act as free radical trapping agents or glutathione peroxidase mimetics, reducing oxidative stress in inflammatory diseases. In this report, we synthesized and screened a library of organoselenium and organotellurium compounds for hydrogen peroxide scavenging activity, using macrophagic cell lines RAW264.7 and THP-1, as well as human mono- and poly-nuclear cells. These cells were stimulated to release H2O2, using phorbol 12-myristate 13-acetate, with and without organochalogens. Released H2O2 was then measured using a chemiluminescent assay over a period of 2 h. The screening identified an organoselenium compound which scavenged H2O2 more effectively than the vitamin E analog, Trolox. We also found that this organoselenium compound protected MC3T3 cells against H2O2 -induced ...
We also decided that we needed to increase the concentration of the HRP in the cuvet. The Fluorimeter was not working and so we just used visual analysis to see if any fluorescence was occurring. The first concentration in the cuvet we tested was 2mM- which did not produce any luminescence. The next concentration we attempted was 10mM which also did not result in any luminescence. We then decided to double this final concentration to 20mM HRP while maintaining 3mM concentration of Luminol. This sample resulted in fluorescence of the luminol in the form of a blue light! We plan on running different variations of the HRP concentration beginning with 20mM tomorrow when conducting the chemiluminescence assay. All other reagents will remain constant ...
Inova Diagnostics, a world leader in autoimmune disease diagnostic systems and reagents for the clinical laboratory, is pleased to announce the 510(k) clearance of QUANTA Flash® Rheumatoid Factor (RF) IgM and QUANTA Flash RF IgA assays by the US Food and Drug Administration (FDA). These chemiluminescent assays complement Inova Diagnostics previously cleared QUANTA Flash CCP3 assay that detects anti-citrullinated peptide antibodies (ACPA) and expands the number of FDA-cleared assays available on BIO-FLASH®, a random access chemiluminescent system.. ...
PerkinElmer, Inc., a global leader committed to innovating for a healthier world, today announced the first two CE-marked chemiluminescence immunoassays (ChLIA) for the detection of anti-Borrelia (Lyme disease) and anti-EBV/EBNA (Epstein-Barr Virus) antibodies on its EUROIMMUN RA Analyzer 10 system.
... luminescent measurements MeSH E05.196.712.516.200 - chemiluminescent measurements MeSH E05.196.712.516.600 - fluorometry MeSH ...
Measurements of the external gamma dose are also carried out. A network of 62 monitoring points with thermal-luminescent ... dosimeters for the measurement of the gamma dose has been established around the power plant and across an area with a radius ...
Spectroscopic measurements indicated virtually no re-absorption losses on distances of tens of centimeters. Photon harvesting ... The luminescent component might be a dopant in the material of some or all of the transparent medium, or it might be in the ... whereas doping with stable inorganic luminescent agents usually is not practical except in inorganic glasses. Luminescent ... Alternatively the luminescent materials can be configured into thin films that emit light into transparent passive media that ...
... for in-vivo electrochemical measurements. They also have potential applications as luminescent probes for the detection of ...
J. P. Feist, J. R. Nicholls, M. J. Fraser, A. L. Heyes (2006) "Luminescent material compositions and structures incorporating ... This allows for temperature measurements and thermal analysis to be performed on intricate and inaccessible components, which ... These technologies utilise the luminescent properties of materials to provide information on past temperatures that they ... SCS has developed two offline temperature measurements: thermal history coatings and thermal history paint. ...
Phosphor thermometry is the measurement technique used for determining the past temperatures of THCs, whereby the luminescent ... The temperature range that THCs provide accurate temperature measurements in is 900 °C to 1400 °C with an accuracy of ±10 °C. ... This allows for point measurements to be made across the coated surfaces of components and allows thermal analysis to be ... Thermocouple Thermocrystal Pyrometer J. P. Feist, J. R. Nicholls, M. J. Fraser, A. L. Heyes (2006) "Luminescent material ...
... and biorhythm assays based on the luminescent detection of ATP. Time-resolved fluorescence (TRF) measurement is very similar to ... The only difference is the timing of the excitation/measurement process. When measuring FI, the excitation and emission ... This results in lower measurement backgrounds than in standard FI assays. The drawbacks are that the instrumentation and ... Lin, Kedan; Sadée, Wolfgang; Mark Quillan, J. (1999-02-01). "Rapid Measurements of Intracellular Calcium Using a Fluorescence ...
... point as confirmed with rope and stone measurement methods by local guides in the area Lake Kaco is reported to be luminescent ...
It was later demonstrated that pure, Cs4PbBr6 NCs were non-luminescent, and that these could be converted to luminescent CsPbX3 ... The lifetime measurements were carried out utilizing both time correlated single photon counting equipment as well as a ... but it is also an indirect-semiconductor and is non-luminescent. The non-luminescent nature of this phase was further ... If the B-X-B angle deviates too far from 180°, phase transitions towards non-luminescent or all-together non-perovskite phases ...
New luminescent phases of strontium vanadate were also discovered during the course of this program. "Lauren Rohwer". Sandia ... This work led to the development of a measurement standard for CL luminous efficiency of phosphor powders and films. She ... Rohwer researches synthesis and characterization of nanoscale luminescent materials with applications to solid-state lighting. ... Rohwer's dissertation was titled Development of efficient, small particle size luminescent oxides using combustion synthesis. ...
A temperature sensor based on direct decay time measurement has been shown to reach a temperature from 1000 to as high as 1,600 ... Early works considered the integration of luminescent materials as erosion sensors in TBCs. The notion of a "thermal barrier ... Therefore, care has to be taken in order to keep constant these parameters for all measurements. A thermal barrier coating (TBC ... Further, temperature measurement capabilities of APS sensor coatings were demonstrated beyond 1400 °C. Results on multilayer ...
... polyaminocarboxylates as luminescent sensors in time-resolved luminescent (TRL) immunoassays. Optimization of analytical ... Measurements can be done under physiological conditions in vitro with genetically encoded dyes, and often in vivo as well. The ... The luminescent probe may for instance serve to localize the MRI contrast agent. This has helped to visualize the delivery of ... Protonation of basic sites in systems comprising a chromophore and a luminescent metal center leads the way for pH sensors. ...
In vitro measurements Other tests measure the antioxidant capacity of a fraction. Some make use of the 2,2'-azino-bis(3- ... Detection can be made by recombinant luminescent bacterial sensors. Phenolic profiling can be achieved with liquid ... Some methods for quantification of total phenolic content are based on colorimetric measurements. Total phenols (or antioxidant ... "Analysis of bioavailable phenols from natural samples by recombinant luminescent bacterial sensors". Chemosphere. 64 (11): 1910 ...
"Basic Instrumentation for Optical Measurement". Fiber Optic Measurement Techniques. pp. 129-258. doi:10.1016/b978-0-12-373865- ... ". "US Patent for Method for manufacturing a metallized luminescent screen for a cathode-ray tube Patent (Patent # 5,800,234 ... Most oscilloscopes have a graticule as part of the visual display, to facilitate measurements. The graticule may be permanently ... Various phosphors are available depending upon the needs of the measurement or display application. The brightness, color, and ...
It has a calculated density of 4.045 g/cm3, and a tested density of 4.00 g/cm3, agreeing favorably, since measurements used to ... Crystals are not luminescent or fluorescent. Jerrygibbsite forms orthorhombic crystals with an imperfect cleavage along the { ...
In luminescent/fluorescent chemosensing these two parts can be 'spaced' out or connected with a covalent spacer. The ... This can be achieved through either a single measurement or through the use of continuous monitoring. The signalling moiety ... F., Callan, J.; P., de Silva, A.; C., Magri, D. (2005). "Luminescent sensors and switches in the early 21st century". ... Ashton, Trent D.; Jolliffe, Katrina A.; Pfeffer, Frederick M. (2015-07-07). "Luminescent probes for the bioimaging of small ...
Quinoline based sensors have been developed that form luminescent complexes with Cd(II) and fluorescent ones with Zn(II). It is ... Fluorophores are essential to our measurement of the metal binding event, and indirectly, metal concentration. There are many ... hypothesized to function by changing its lowest luminescent state from n-π* to π-π* when coordinating to a metal. When the ...
As a result, for crystalline ML materials, XRD measurement may not able to detect changes before and after mechanical stimuli ... Mechanochromic luminescence (ML) references to intensity and/or color changes of (solid-state) luminescent materials induced by ... "A mechanistic investigation of mechanochromic luminescent organoboron materials". Journal of Materials Chemistry. 22 (33): ...
Since luminescent ink or luminescent paper are only delivered to specialist printers, tagging also serves as an anti- ... Measurement and Instrumentation, Management and Education, Reviews. 128 (2): 107-116. Kevin Danchisko. "Machines or Bust: Post ... Deutsche Post of the GDR did not use luminescent tagging on stamps. Luminescent tagging has been added to postage stamps of the ... Tagging of postage stamps means that the stamps are printed on luminescent paper or with luminescent ink to facilitate ...
... was a French scientist and a pioneer in the study of electric and luminescent phenomena. He was born at Châtillon-sur-Loing ( ... In 1825 he invented a differential galvanometer for the accurate measurement of electrical resistance. In 1829 he invented a ...
Experiments using luminescent Photobacterium leiognathi and non-luminescent mutants have shown that luminescence attracts ... The applications of bioluminescent bacteria include biosensors for detection of contaminants, measurement of pollutant toxicity ... Nevertheless, all bio-luminescent bacteria share a common gene sequence: the enzymatic oxidation of Aldehyde and reduced Flavin ... Of all light emitters in the ocean, bio-luminescent bacteria is the most abundant and diverse. However, the distribution of bio ...
Nanothermometers are classified as luminescent thermometers (if they use light to measure temperature) and non-luminescent ... These measurements are used to initialize weather forecast models. Thermometers are used in roadways in cold weather climates ... R.P. Benedict (1984) Fundamentals of Temperature, Pressure, and Flow Measurements, 3rd ed, ISBN 0-471-89383-8, page 5 J. Lord ( ... Galileo thermometers are used to measure indoor air temperature, due to their limited measurement range. Such liquid crystal ...
Y 2SiO 5:Ce3+ degrades by loss of luminescent Ce3+ ions. Zn 2SiO 4:Mn (P1) degrades by desorption of oxygen under electron ... Phosphor thermometry is a temperature measurement approach that uses the temperature dependence of certain phosphors. For this ... Postage stamps are sometimes collected by whether or not they are "tagged" with phosphor (or printed on luminescent paper). ... to create luminescent paint for dials of watches and instruments (radium dials). Between 1913 and 1950 radium-228 and radium- ...
... performance recording automated machine action altered through measurement segregation/rejection according to measurement ... and thus even more expensive when compared to wind energy harvesters or luminescent solar concentrators). For simplification, ... measurement selected signaling control, e.g. hydro power control ...
However, the direct measurement of nitrogen in the platelets by EELS (an analytical technique of electron microscopy) revealed ... it was noted that not all dislocations are luminescent, and there is no correlation between the dislocation type and the ... Tucker, O.; Newton, M.; Baker, J. (1994). "EPR and N14 electron-nuclear double-resonance measurements on the ionized nearest- ... Twitchen, D.; Newton, M.; Baker, J.; Anthony, T.; Banholzer, W. (1999). "Electron-paramagnetic-resonance measurements on the ...
... in which a single measurement is performed after a fixed incubation period; or A kinetic assay, in which measurements are ... MTT assay Cell Counting Kit-8 (WST-8 based cell viability assay) SRB (Sulforhodamine B) assay CellTiter-Glo® Luminescent Cell ... Nephelometry where a measurement of the amount of light scattering that occurs when a beam of light is passed through the ... Viscoelastic measurements e.g. viscometry, elastography (e.g. thromboelastography) Counting assays: e.g. optic Flow cytometric ...
Turro, N.J.; Yekta, A. (1978). "Luminescent probes for detergent solutions. A simple procedure for determination of the mean ... 2)". In Bills, Donald D.; Mussinan, Cynthia J. (eds.). Characterization and Measurement of Flavor Compounds. ACS Symposium ...
Earlier sound measurements did not meet the standards in place in 2002. According to Small Arms Review, the lower earlier ... The Welrod is provided with sights marked with luminescent paint for use in low light conditions. Regarding operating range, ... measurements were 'Undoubtedly […] a function of the available measuring equipment (including excessive meter rise time)'. A ...
The luminescent glow is concentrated on the surface of the ocean and does not mix evenly throughout the water column. In 1985, ... "Observations and measurements of planktonic bioluminescence in and around a milky sea". Journal of Experimental Marine Biology ... The luminescent field was observed to glow over three consecutive nights. While monochromatic photos make this effect appear ...
It makes use of histograms of images captured by a camera in real-time and does not make use of any distance measurements to ... The algorithm utilizes agents called glowworms which use a luminescent quantity called Luciferin to (indirectly) communicate ...
G. Dmitri, M. Mitome, K. Kurashima, Y. Bando : «In situ electrical measurements and manipulation of B/N-dopped C nanotubes in a ... X. Li, N. Hanagata, X. Wang, M. Yamaguchi, W. YI, Y. Bando, D. Golberg : «Multimodal luminescent-magnetic boron nitride ... N. Kawamoto, M. Wang, X. Wei, D. Tang, Y. Murakami, D. Shindo, M. Mitome and D. Golberg : «Local temperature measurements on ... B. Liu, Y. Bando, M. Wang and D. Golberg : «Synthesis and In-Situ TEM Transport Measurements of Individual GaN Nanowires and ...
Measurement[edit]. Main article: Photometry (optics). Measurement of light or photometry is generally concerned with the amount ... Typical measurements of light have used a Dosimeter. Dosimeters measure an individual's or an object's exposure to something in ... Several measurement methods have been developed to control glare resulting from indoor lighting design. The Unified Glare ... The basic SI unit of measurement is the candela (cd), which describes the luminous intensity, all other photometric units are ...
Invisible or poorly visible prints can be further enhanced by applying a luminescent or non-luminescent stain. ... There is no singular measurement of toxicity for all cyanoacrylate adhesives because of the large number of adhesives that ...
Lab color measurements, unless otherwise noted, assume that the measurement was taken under a D65 light source, or "Daylight ... Dutch and Flemish painters of the 17th and 18th centuries favored it for its luminescent qualities, and often used it to ... Averaged measurements of pigment samples will only yield approximations of their true appearance under a specific source of ... However, by making careful measurements of pigments, close approximations can be made. The Munsell Color System provides a good ...
Plasma scattering of electromagnetic radiation : theory and measurement techniques. Froula, Dustin H. (1st ed., 2nd ed.). ... luminescent lamps,[64] fuel ignition, while even playing a part in supersonic combustion engines for aerospace engineering.[71] ...
... or antibiotic susceptibility testing is the measurement of the susceptibility of bacteria to ... The isolate is then exposed to fluoresent dye, which will be luminescent when viewed.[9] ...
"Water Vapor Measurements with LED Detectors". cs.drexel.edu (2002). *^ Dziekan, Mike (February 6, 2009) "Using Light-Emitting ... Ben-Ezra, Moshe; Wang, Jiaping; Wilburn, Bennett; Xiaoyang Li; Le Ma (2008). "An LED-only BRDF measurement device". 2008 IEEE ... Luminescent. *Fluorescent *Fluorescent lamp (compact). *Fluorescent induction. *Photoluminescent *laser lamp. *Chemiluminescent ...
A measurement unit called CRI is used to express how the light source's ability to render the eight color sample chips compare ... Luminescent. *Fluorescent *Fluorescent lamp (compact). *Fluorescent induction. *Photoluminescent *laser lamp. *Chemiluminescent ...
Xavier PL, Chaudhari K, Verma PK, Pal SK, Pradeep T (2010). "Luminescent quantum clusters of gold in transferrin family protein ... A rapid, portable test utilizing microfluidic technology has been developed to enable measurement of lactoferrin levels in ...
... synthetic and imitation diamonds are most commonly distinguished using optical techniques or thermal conductivity measurements ... Luminescent minerals. *Industrial minerals. Hidden categories: *Articles with short description. *Wikipedia indefinitely semi- ... "High spatial resolution photoluminescence and Raman spectroscopic measurements of a natural polycrystalline diamond, carbonado ...
Detection can be made by recombinant luminescent bacterial sensors.[33] Profiling[edit]. Phenolic profiling can be achieved ... In vitro measurements. Other tests measure the antioxidant capacity of a fraction. Some make use of the 2,2'-azino-bis(3- ... Some methods for quantification of total phenolic content are based on colorimetric measurements. Total phenols (or antioxidant ... "Analysis of bioavailable phenols from natural samples by recombinant luminescent bacterial sensors". Chemosphere. 64 (11): 1910 ...
... synthetic and imitation diamonds are most commonly distinguished using optical techniques or thermal conductivity measurements ... Luminescent minerals. *Industrial minerals. Hidden categories: *Articles with imported freely licensed text ... "High spatial resolution photoluminescence and Raman spectroscopic measurements of a natural polycrystalline diamond, carbonado ...
Different measurements of solar irradiance (direct normal irradiance, global horizontal irradiance) are mapped below : ... Luminescent solar concentrators (when combined with a PV-solar cell) can also be regarded as a CPV system. Concentrated ... "What is a luminescent solar concentrator?". Science.howstuffworks.com. Archived from the original on 10 March 2010. Retrieved ...
IEEE Instrumentation & Measurement Magazine. 14 (5): 41-48. doi:10.1109/MIM.2011.6041381. Archived (PDF) from the original on ... and this principle is used for very accurate measurements of very small mass changes in the quartz crystal microbalance and in ... Luminescent minerals. *Quartz gemstones. *Industrial minerals. *Silica polymorphs. *Symbols of South Dakota ... "The evolution of time measurement, Part 2: Quartz clocks [Recalibration]" (PDF). ...
Luminescent radium was used to paint watches and other items that glowed. The most famous incident is the "Radium Girls" of ... It is believed that the capsule, originally contained in a measurement device, was lost sometime during the late 1970s and ...
... is an optical and electromagnetic phenomenon in which electrons impacting on a luminescent material such as ... to picosecond-level time-resolved measurements if the electron beam can be "chopped" into nano- or pico-second pulses by a beam ...
... portable test utilizing microfluidic technology has been developed to enable measurement of lactoferrin levels in human tear ... "Luminescent quantum clusters of gold in transferrin family protein, lactoferrin exhibiting FRET" (PDF). Nanoscale. 2 (12): ...
... luminescent sub-cellular vesicles that he believed were observable in decaying materials, and presumably present universally. ... http://nccih.nih.gov/health/backgrounds/energymed.htm "putative energy fields (also called biofields) have defied measurement ...
Integrating cavity measurements". Applied Optics. Optical Society of America. 36 (33): 8710-8723. doi:10.1364/AO.36.008710. ... Owing to their spiky distribution, much finer increments are advisable for taking measurements of fluorescent lights, and this ... Luminescent. *Fluorescent *Fluorescent lamp (compact). *Fluorescent induction. *Photoluminescent *laser lamp. *Chemiluminescent ...
By applying small voltages to the leads, current through the quantum dot can be controlled and thereby precise measurements of ... Sankaran, R. M.; Holunga, D.; Flagan, R. C.; Giapis, K. P. (2005). "Synthesis of blue luminescent Si nanoparticles using ... Norris, D.J. (1995). "Measurement and Assignment of the Size-Dependent Optical Spectrum in Cadmium Selenide (CdSe) Quantum Dots ... Mangolini, L.; Thimsen, E.; Kortshagen, U. (2005). "High-yield plasma synthesis of luminescent silicon nanocrystals". Nano ...
The impact of the Cultural Revolution on accessible education varied among regions, and formal measurements of literacy did not ... and luminescent".[146][148] ...
Two very similar measurement systems were created to bridge the scotopic and photopic luminous efficiency functions,[43][44][45 ... creating a Unified System of Photometry.[46][47] This new measurement has been well-received because the reliance on V(λ) alone ... Luminescent. *Fluorescent *Fluorescent lamp (compact). *Fluorescent induction. *Photoluminescent *laser lamp. *Chemiluminescent ...
Blinks JR, Wier WG, Hess P, Prendergast FG (1982). "Measurement of Ca2+ concentrations in living cells". Prog Biophys Mol Biol ... Shimomura O, Johnson FH, Morise H (1974). "Mechanism of the luminescent intramolecular reaction of aequorin". Biochemistry. 13 ... Rembold CM, Kendall JM, Campbell AK (January 1997). "Measurement of changes in sarcoplasmic reticulum [Ca2+] in rat tail artery ... Mithöfer A, Mazars C (2002). "Aequorin-based measurements of intracellular Ca2+ signatures in plant cells". Biol. Proced. ...
LEED is a measurement tool for green building in the United States and it is developed and continuously modified by workers in ... "Highly efficient luminescent solar concentrators based on earth-abundant indirect-bandgap silicon quantum dots, Nature ... LEED is a design tool and not a performance measurement tool. It is also not yet climate-specific, although the newest version ... Also to create the "luminescent discovery" for solar concentrators as the innovation by using nanoparticle technology has been ...
... pioneer in the study of electric and luminescent phenomena Henri Becquerel (1852-1908) - awarded the Nobel Prize in physics for ... Astronomer who pioneered the application of photoelectric methods to the measurement of the brightness of celestial bodies ...
During the dive, measurement instruments (gauges, dive computers, compass, etc.) are available to cross-check one another, a ... The octopus is usually clearly marked, the convention is a yellow hose and yellow second stage though a luminescent green is ...
The luminescent in vivo glucose measurement method includes the steps of illuminating displaced luminescent molecules with ... molecules and associated captive glucose analogue molecules being contained within an implanted luminescent in vivo measurement ... A luminescent in vivo glucose measurement method and apparatus is provided. ... 6. The luminescent in vivo glucose measurement method of claim 1. , wherein said luminescent in vivo measurement apparatus is ...
... Martha OBrien1, Mike Scurria2, Laurent Bernad ... Measurement-of-Three-Proteasome-Proteolytic-Activities-Using-Luminescent-AssaysPDF. (. 127. KB. ). - English ...
"Luminescent Measurements" by people in this website by year, and whether "Luminescent Measurements" was a major or minor topic ... "Luminescent Measurements" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... Below are the most recent publications written about "Luminescent Measurements" by people in Profiles. ... Below are MeSH descriptors whose meaning is more general than "Luminescent Measurements". ...
The effect of luminescent coupling on modulated photocurrent measurements in multijunction solar cells. August 21, 2019 ... Luminescent coupling in multijunction solar cells has a major impact on device response and its impact on current-voltage and ... quantum efficiency measurements is well established. However, the role of luminescent coupling in more advanced ... accurate methods for proper measurements of the current vs voltage characteristics of light-harvesting solar photovoltaic ...
Luminescent Measurements * Neutrophils / cytology * Neutrophils / immunology* * Neutrophils / metabolism * Phagocytosis / drug ...
Luminescent Measurements * Male * Methacholine Chloride * Nitric Oxide / metabolism* * Respiratory Function Tests * Respiratory ...
Luminescent Measurements. *Mass Spectrometry. *Pulse Radiolysis. *Spectrophotometry. *Spectrophotometry, Ultraviolet. Research ...
Luminescent Measurements. Lysosomal Storage Diseases/*diagnosis. Malonates/blood. Mercury. Mouth Mucosa. Mutagenicity Tests ...
Luminescent in vivo glucose measurement. WO2005011520A2. 21 Jul 2004. 10 Feb 2005. Dexcom, Inc.. Oxygen enhancing membrane ... Non-invasive measurement of analytes. US20070027384 *. 4 Oct 2006. 1 Feb 2007. Mark Brister. Dual electrode system for a ... Non-invasive measurement of analytes. US8512239. 20 Apr 2009. 20 Aug 2013. Abbott Diabetes Care Inc.. Glucose measuring device ... Non-invasive measurement of analytes. US20040152187 *. 6 Nov 2003. 5 Aug 2004. Haight Levoy Golden. System, apparatus and ...
Simultaneous velocity and pressure measurements using luminescent microspheres.. Kimura F, McCann J, Khalil GE, Dabiri D, Xia Y ... Continuous measurement of oxygen consumption by pancreatic islets.. Sweet IR, Khalil G, Wallen AR, Steedman M, Schenkman KA, ... Measurement of respiration rates of Methylobacterium extorquens AM1 cultures by use of a phosphorescence-based sensor. ...
Bromides; Crystallization; Electron Spin Resonance Spectroscopy; Luminescent Agents; Luminescent Measurements; Microscopy, ...
Luminescent Measurements. Male. Monocytes / metabolism, physiology*. Neutrophils / metabolism, physiology*. Pneumonia / blood* ...
Luminescent Measurements. Male. Oligomenorrhea / blood, diagnosis*. Oligospermia / blood, diagnosis. Polycystic Ovary Syndrome ... The FAI may be a cost-effective alternative to free T measurement in the diagnosis of oligomenorrhea and hirsutism in women as ...
Dissolved oxygen measurements were collected using a luminescent sensor. Nitrate measurements were collected using a Hach ...
Luminescent decay time techniques for temperature measurement. US4476870. 7 Jul 1982. 16 Oct 1984. The United States Of America ... Some of the light is absorbed by the luminescent chemical dye in the film whereupon it produces luminescent light at a second, ... The luminescent chemical sensor is not toxic to the patient and is a part of a consumable (i.e., disposable) airway adapter ... Luminescent oxygen sensor based on a lanthanide complex. US4892383. 17 Feb 1989. 9 Ene 1990. Fiberchem Inc.. Reservoir fiber ...
Multiplex measurements of multiple luminescent species. Overview of the Nano-Glo® In-Gel Detection System. ... Cells transfected with carrier DNA alone showed no luminescent background bands, while transfected cells showed fusion proteins ...
Temperature measurement with combined photo-luminescent and black body sensing techniques. US5196694 *. May 13, 1991. Mar 23, ... However, a second source is added in order to provide measurement over a range greater than the 500 C. limit discussed above. ... Not only does this widen the range of direct applications where the sensor can be used for temperature measurement, but it also ... J.W. Berthold, S.E. Reed and R.G. Sarkis, "Simple, Repeatable, Fiber Optic Intensity Sensor for Temperature Measurement", SPIE ...
Measurement of interchain and intrachain exciton hopping barriers in luminescent polymer. Journal of Physics: Condensed Matter ... Dias, FB, Maiti, M, Hintschich, SI & Monkman, AP (2005). Intramolecular fluorescence quenching in luminescent copolymers ... O Ligands and Their Highly Luminescent Tetradentate Pt(II) Complexes for Solution-Processable OLEDs. Inorganic Chemistry 57(7 ... containing fluorenone and fluorene units: A direct measurement of intrachain exciton hopping rate. Journal Of Chemical Physics ...
9781118384633 Our cheapest price for Handbook of Measurement in Science and Engineering, Two Volume Set is $435.27. Free ... 16 Luminescent Method for Pressure Measurement 567. Gamal E. Khalil, Jim W. Crafton, Sergey D. Fonov, Marvin Sellers, and Dana ... 35.2 Stationary Methods for Measurement of the Thermal Conductivity, 1157. 35.3 Transient Methods for the Measurement of the ... 48.6 Resistance Bridge Transducer Measurement System Calibration, 1636. 48.7 Resistance Bridge Transducer Measurement System ...
Ullman, Edwin F. et al., "Luminescent oxygen channeling immunoassay: Measurement of particle binding kinetics by ... 1994; and Measurement of mouse and human Interferon gamma, Schreiber, R. D. In Current Protocols in Immunology. J. E.e.a. ... Methods of determining patient response by measurement of her-3. US20100215584 *. Nov 29, 2006. Aug 26, 2010. Passe James G. ... Methods of Determining Patient Response By Measurement of HER-2 Expression. US20110050431 *. Sep 2, 2009. Mar 3, 2011. Hood ...
Dynamic strain measurements with a luminescent photoelastic coating Author(s): Daniel R. Gerber; Hisham K. Ali; James P. Hubner ... shear velocity measurements along with the corresponding attenuation measurements are presented. The measurements were carried ... Pulse-echo measurements confirmed the reflected echoes from the specimen. Such measurement was not possible with commercial UTs ... Optimal measurement location selection problem and comparison of power-meter and CCD camera based sensor measurements were both ...
A luminescent assay for real-time measurements of receptor endocytosis in living cells. ... A luminescent biosensor with increased dynamic range for intracellular cAMP.. Binkowski BF, Butler BL, Stecha PF, Eggers CT, ... Quantitative, Real-Time Measurements of Intracellular Target Engagement Using Energy Transfer.. Robers MB, Vasta JD, Corona CR ... CRISPR-Mediated Tagging of Endogenous Proteins with a Luminescent Peptide.. Schwinn MK, Machleidt T, Zimmerman K, Eggers CT, ...
For example, a novel confocal luminescent lifetime measurement method, says Yean-Sheng. This method is non-destructive, does ... During early experiments, our measurements didnt always match our expectations, Yean-Sheng shares. ... to find a good balance between performing numerical analyses and setting up new sets of experimental devices and measurements. ...
Investigatin of a luminescent based technique for surface temperature measurements. p. 393. ... Ultrasonic tensiographic measurements on liquid drops for liquid fingerprinting. p. 297. A dual element optical fibre water ... Surface roughness measurement using fibre grating assisted fibre interferometer. p. 209. Interferometric interrogation of in ... Concurrent measurement of mass flow rate and size distribution of pneumatically conveyed particles using combined electrostatic ...
Luminescent Measurements (methods) *Male. *Middle Aged. *Recombinant Proteins (administration & dosage, pharmacokinetics) * ...
Viability measurements were made using CellTiter-Glo Luminescent Cell Viability Assay (Promega) per manufacturers suggestions. ... Proliferation measurement was after 100 h and migration measurement was after 24 h. Mean ± standard deviation with n = 8. (b) ... End point measurement lysates were diluted at both 25× and 10× in assay diluent to a final volume of 25 μL per well with data ... a) (left panel) End point lysate measurements and (right panel) end point percent shed (rf/rt) levels normalized to the mean ...
Phase shift measurement for luminescent light US20040150819A1 (en) * 2003-02-03. 2004-08-05. Inberg Steven Bradley. Phase shift ... Phase shift measurement for luminescent light US20050012502A1 (en) * 2003-06-02. 2005-01-20. Wolfgang Renz. Magnetic resonance ... measurement for luminescent light US20050012502A1 (en) * 2003-06-02. 2005-01-20. Wolfgang Renz. Magnetic resonance system with ... Real time in vivo measurement of temperature changes with NMR imaging US6078176A (en) 2000-06-20. Fast spin echo pulse sequence ...
Digital LCD plus analog bargraph; Electro-luminescent lamp for viewing displayed measurements at very low light levels ... Measurement results are displayed on an Electroluminescent backlit Custom LCD. It has a digital f/stop range from f/0.35 to f/ ... f/stop difference: the difference in f/stop between a new reading and the last memorized measurement is displayed in 1/10th ... This compact and user-friendly light meter provides an easy, economical means of making highly accurate measurement of indoor ...
Luminescent in vivo glucose measurement US6485703B1 (en) * 1998-07-31. 2002-11-26. The Texas A&M University System. ... Luminescent in vivo glucose measurement US6846638B2 (en) * 2000-08-10. 2005-01-25. Nanobiodynamics, Inc.. Method and system for ... Non-invasive measurement of blood glucose US5101814A (en) * 1989-08-11. 1992-04-07. Palti Yoram Prof. System for monitoring and ... Non-invasive measurement of blood glucose US5101814A (en) * 1989-08-11. 1992-04-07. Palti Yoram Prof. System for monitoring and ...
Wavelength, space, and time resolved fluorescence measurements allowed the luminescent areas to be monitored on whole bananas. ... In luminescent rings on banana peels hypermodified FCCs accumulate. (A) HPLC-analysis of FCCs in luminescent rings and dark ... In Vivo Fluorescence Maps of Luminescent Rings.. The development of the blue luminescent rings with time was further monitored ... blue luminescent rings develop. As reported here, the luminescent rings form blue halos around the "dark" spots on the peel of ...
  • Measurements were made using optically-stimulated luminescent dosimeters (OSLDs), composed of carbon-doped aluminum oxide, with characteristics suitable for radiotherapy dosimetry studies. (medworm.com)
  • The ASTRO presentation is based on Dr. Jursinic's independent research and 2007 article in the Journal of Medical Physics, "Characterization of optically stimulated luminescent dosimeters, OSLDs, for clinical dosimetric measurements," which concludes that OSLDs may be a superior substitute for TLDs and diodes for in vivo dosimetric measurements, particularly for surface dose measurements. (redorbit.com)
  • Dissolved oxygen measurements were collected using a luminescent sensor. (usgs.gov)
  • Being able to act on accurate measurement of Dissolved Oxygen in activated sludge plants will enable you to maintain levels of bacterial activity, avoid breaches in discharge consents and operate as cost effectively as possible. (environmental-expert.com)
  • The OxyTechw² GAL Sensor has been designed to provide highly reliable Dissolved Oxygen measurements. (environmental-expert.com)
  • The 9065 Luminescent Dissolved Oxygen Analyzer utilizes new luminescent technology for measuring dissolved oxygen in water at sub ppb levels. (ecmconnection.com)
  • Luminescent technology features superior benefits and cost savings compared to traditional dissolved oxygen sensing technologies. (ecmconnection.com)
  • The new measurement principle (LDO stands for Luminescent Dissolved Oxygen) has revolutionised oxygen measurement. (uniquegroup.com)
  • The ProODO™ handheld meter provides durability for the measurement of optical, luminescent-based dissolved oxygen for any field application. (thomassci.com)
  • patented Luminescent Dissolved Oxygen (LDO) technology. (thomassci.com)
  • The LBOD101 is a digital, luminescent dissolved oxygen (LDO) sensor with a replaceable, integrated stirring system. (thomassci.com)
  • Comprised of five main section, the book covers theoretical and very simple suspension-based fluorimetric assays, specialist measurement systems, measurement of channel activity, measurement of store release, as well as specialist measurement techniques which include targeted probes, using G-protein chimeras to force Ca 2+ signalling for screening, and genetically encoded sensors. (springer.com)
  • Originally developed as a methodology for diagnostic assays called LOCI ® (Luminescent Oxygen Channeling Immunoassay) in 1994 1 , today the AlphaScreen Technology (Alpha Technology) includes AlphaScreen, AlphaLISA ® and AlphaPlex TM . (bmglabtech.com)
  • A method for performing time resolved homogeneous assays using a long-lifetime luminescent dye as a donor. (patentgenius.com)
  • Ripening bananas exhibit blue luminescence and develop strongly luminescent blue halos around senescence associated dark spots. (pnas.org)
  • Every single measurement contained 2,500 pH values due to the luminescence imaging technique used. (uni-muenchen.de)
  • 3. A method for detecting an luminescence comprising: reacting a compound according to claim 1 with luminescent beetle luciferase, and detecting an luminescence from the compound. (patentgenius.com)
  • It is known that this bioluminescence of the firefly emit a luminescence by chemically reacting with the luciferin which is luminescent substrate through the carton of the luciferase which is luminescent enzyme. (patentgenius.com)
  • Landauer, Inc. (NYSE: LDR), a recognized leader in personal and environmental radiation monitoring, today announced that noted medical physicist, Paul A. Jursinic, Ph.D., will be presenting at ASTRO his findings on the superiority of Landauer's Optically Stimulated Luminescence Dosimetry (OSLD) for clinical dosimetric measurements. (redorbit.com)
  • As a contamination index, ATP measurement was taken by luminescence with a luminometer (lumitester K-210) on the solution made of lOOmul of specimen liquid and lOOmul of ATP extract reagent with addition of ATP luminescent reagent (luciferin - luciferase) Areas measured were tip and handle portions of instruments and equipment, the face and chest surface of the operator, assistant and patient, and left and right forearms of the operator and assistant. (nii.ac.jp)
  • AlphaScreen ® (ALPHA for Amplified Luminescent Proximity Homogeneous Assay) is a bead-based chemistry used to study interactions between molecules in a microplate. (bmglabtech.com)
  • In this application protocol we demonstrate the use of the modified luminescent firefly luciferase-based Promega GloSensor™ cAMP Assay on the FLIPR Tetra ® High Throughput Cellular Screening System to enable detection of cAMP-mediated G s - and G i -coupled GPCR activity in kinetic mode. (moleculardevices.com)
  • For comparison, a luminescent assay using Firefly luciferase was utilized. (news-medical.net)
  • Laboratory methodology Serum ferritin was measured by chemi-luminescent micro-particle immunoassay on Cobos® E-601 and hemoglobin was measured using Sysmex® XP-100. (clinicaltrials.gov)
  • Nitrate measurements were collected using a Hach Nitratax sensor with 2 mm wavelength sensing. (usgs.gov)
  • Methods: Time domain dual lifetime referencing with luminescent sensor foils was used for pH(SS) measurements. (uni-muenchen.de)
  • The Hach LDO sensor works using a coating of luminescent material. (ferret.com.au)
  • Between the flashes of blue light a red LED is flashed on the sensor and used as an internal reference, essentially validating each measurement. (ferret.com.au)
  • Wavelength, space, and time resolved fluorescence measurements allowed the luminescent areas to be monitored on whole bananas. (pnas.org)
  • Fluorescence measurements are performed when greater sensitivity is needed. (news-medical.net)
  • In the case of fluorescence measurements, it is essential to quench the auto-fluorescence emitted by the polystyrene substrate and this can be achieved by adding 1% carbon black to the polystyrene. (news-medical.net)
  • The effects of excitation wavelength on the optical properties (emission spectrum and quantum yield) of a luminescent solar concentrator (LSC) containing a fluorescent organic dye (Lumogen F Rot 305) are studied. (osapublishing.org)
  • The temperature is obtained by evaluating the emission decay of individual luminescent polymer particles, whereas the velocity field can be calculated simultaneously from the flow-induced shift of individual particle images in time. (springer.com)
  • Per definition, luminescent light stems from cold sources and is differentiated from light emission from heated sources such as hot iron or a burning candle 1 . (bmglabtech.com)
  • The latter one is what we see as luminescent emission 2 . (bmglabtech.com)
  • The optical emission spectroscopy (OES) in situ measurement revealed the kinetics species variation during rf plasma deposition process. (hindawi.com)
  • These dsDs have the potential to become new-generation luminescent probes for super-multiplex biological analysis without disturbance from short lifetime background emission. (sciencemag.org)
  • Besides, Luís Carlos said, luminescent nanoparticles (quantum dots and nanocrystals with lanthanide ions) emerge in diagnostic techniques and also in treatments, such as the hyperthermia process. (sbpmat.org.br)
  • DJL returned to the University of Birmingham to work under Professor Zoe Pikramenou, developing luminescent nanoparticles for flow velocimetry measurements in collaboration with Professor Panagiota Angeli, University College London. (rsc.org)
  • As is well known, the bioluminescent system of firefly shows great efficiency in luminescent, and it is said that this system is one of the most efficiently systems that can convert energy into a light. (patentgenius.com)
  • The development of modern biological and medical science highly depends on advanced luminescent probes. (sciencemag.org)
  • Augustsson P, Barnkob R, Wereley ST, Bruus H, Laurell T (2011) Automated and temperature-controlled micro-PIV measurements enabling long-term-stable microchannel acoustophoresis characterization. (springer.com)
  • Radiation Measurements provides a forum for the presentation of the latest developments in the broad field of ionizing radiation detection and measurement. (elsevier.com)
  • Radiation Measurements, 90, 132−135.doi:10.1016/j.radmeas.2015.12.032. (etis.ee)
  • Radiation Measurements, 90, 43−46.10.1016/j.radmeas.2016.01.007. (etis.ee)
  • The chelates find particular use in resonance energy transfer between chelate-lanthanide complexes and another luminescent agent, often a fluorescent non-metal based resonance energy acceptor. (google.co.uk)
  • 7. The luminescent in vivo glucose measurement method of claim 1 , wherein said illuminating light is of a first wavelength and said emitted light is of a second wavelength. (google.com.au)
  • 9. The luminescent in vivo glucose measurement method of claim 1 , wherein a wavelength of said illuminating light is in a range of about 800 nanometers to about 2000 nanometers. (google.com.au)
  • 10. The luminescent in vivo glucose measurement method of claim 1 , wherein a wavelength of said illuminating light is about 830 nanometers and a wavelength of said emitted light is about 900 nanometers. (google.com.au)
  • two light sources of different wavelengths, a first wavelength being selected to provide a temperature measurement for a lower temperature range of -100 C. to 400 C. and a second wavelength being selected to provide a temperature measurement for a higher temperature range of from 400 C. to 900 C. (google.com)
  • Herein, we revealed a symmetry-breaking charge transfer (SBCT) process in the excited state of a directly linked push-pull porphyrin dyad (AD) and triad (ADA) via spectroscopic measurements including steady-state absorption and fluorescence, time-resolved fluorescence (TRF), femtosecond transient absorption (fs-TA), and time-resolved infrared (TRIR) measurements. (bioportfolio.com)
  • The importance of using this method for further polarization investigations to increase our understanding of the structure and spectroscopic properties of complex luminescent guest molecules, as well as the internal structure and motions of different host polymers, is pointed out. (osapublishing.org)
  • accident and retrospective dosimetry (including activation detectors), and dosimetry and measurements related to medical applications. (elsevier.com)
  • 6. The luminescent in vivo glucose measurement method of claim 1 , wherein said luminescent in vivo measurement apparatus is implanted about three to about four millimeters below an outer surface of a patient's skin. (google.com.au)
  • 11. The luminescent in vivo glucose measurement method of claim 1 , wherein said illuminating light travels from outside said subject, through said subject's skin, and into said luminescent in vivo measurement apparatus. (google.com.au)
  • 2. The luminescent in vivo glucose measurement method of claim 1 , wherein said glucose level in said interstitial fluid is substantially equal to a blood glucose level. (google.com.au)
  • Recovery of intrinsic fluorescence from single-point interstitial measurements for quantification of doxorubicin concentration. (rochester.edu)
  • The lower the ATP concentration ( = the degree of pollution) is, the lower the luminescent measurement value (RLU) is. (nii.ac.jp)
  • The luminescent in vivo glucose measurement method includes the steps of illuminating displaced luminescent molecules with illuminating light, the displaced luminescent molecules and associated captive glucose analogue molecules. (google.com.au)
  • 4. The luminescent in vivo glucose measurement method of claim 1 , wherein said displaced luminescent molecules are inorganic. (google.com.au)
  • Luminescent organic complexes, for example, are marketed as contrast agents for magnetic resonance imaging, and as markers for fluoroimmunoassays (used in pre- and neonatal screening and detection of proteins, viruses, antibodies, drug residues etc. (sbpmat.org.br)
  • 5 .Charge Pumping Measurement for Organic Diodes, Anuj Singal (EE). (iitk.ac.in)
  • Abram C, Pougin M, Beyrau F (2016) Temperature field measurements in liquids using ZnO thermographic phosphor tracer particles. (springer.com)
  • A luminescent in vivo glucose measurement method and apparatus is provided. (google.com.au)
  • 3. The luminescent in vivo glucose measurement method of claim 1 , wherein said method is used to measure a glucose level in a bloodstream of said subject. (google.com.au)
  • 8. The luminescent in vivo glucose measurement method of claim 1 , wherein said illuminating light is an infrared light. (google.com.au)
  • Cell health and viability measurements provide essential insight into a broad range of biological processes and treatment responses. (biotek.com)
  • Luminescent and structural properties of ZnxMg1-xWO4 mixed crystals. (etis.ee)
  • In particular embodiments, no laser is needed if the donor fluorophore is a luminescent molecule or is stimulated by one. (freepatentsonline.com)
  • In some plants, FCCs are uniquely persistent, as is seen in bananas, and can thus be used as luminescent in vivo markers in tissue undergoing senescence. (pnas.org)
  • Dominantly black, it offers a nice contrast with highly luminescent markers. (watchalyzer.com)
  • Above that I learnt to find a good balance between performing numerical analyses and setting up new sets of experimental devices and measurements. (utwente.nl)
  • The numerical modeling results and the experimental measurements are in excellent agreement for the different parameters studied in this paper. (spie.org)
  • In this work, we first proposed a comprehensive numerical simulation for luminescent coupling efficiency (LCE) in a complete multi-junction (MJ) configuration and discussed its dependencies on temperature, incident light spectrum and bias voltage. (spiedigitallibrary.org)
  • Fast Multisite Optical Measurement of Membrane Potential, with Two Examples. (elsevier.com)
  • Improved membrane life assures measurement consistency and speeds maintenance. (thomassci.com)
  • When a model does not fit the calibration measurements well, we face the difficult choice between readjusting model parameters and re-measuring wafer CDs of calibration patterns. (spie.org)
  • A growing number of tools now allow live recordings of various signaling pathways and protein-protein interaction dynamics in time and space by ratiometric measurements, such as Bioluminescence Resonance Energy Transfer (BRET) Imaging. (frontiersin.org)
  • Accurate and reproducible analysis of ratiometric measurements has thus become mandatory to interpret quantitative imaging. (frontiersin.org)
  • Each offers advantages for the reproducible measurement of cell proliferation. (jove.com)
  • Quantitative, Real-Time Measurements of Intracellular Target Engagement Using Energy Transfer. (nih.gov)
  • Our studies revealed an accumulation of FCCs in luminescent rings, within senescing cells undergoing the transition to dead tissue, as was observable by morphological textural cellular changes. (pnas.org)
  • The FAI may be a cost-effective alternative to free T measurement in the diagnosis of oligomenorrhea and hirsutism in women as previously shown, but may have little relevance in men. (biomedsearch.com)
  • Being an expert in luminescent materials, which emit light not derived from heat, the scientist showed in his plenary lecture that these materials are already of great use in medical diagnosis. (sbpmat.org.br)
  • Luminescent Measurements" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (uchicago.edu)
  • These dynamics were also confirmed via TRIR measurements, which provided further information on the solvation and structural relaxation processes of the SBCT process. (bioportfolio.com)
  • Funatani S, Fujisawa N, Ikeda H (2004) Simultaneous measurement of temperature and velocity using two-colour LIF combined with PIV with a colour CCD camera and its application to the turbulent buoyant plume. (springer.com)
  • and Materials Properties and Testing, Instrumentation, and Measurement Standards in Volume II. (ecampus.com)
  • The needs for apparatuses and labeling materials with higher-sensitivity and higher-performance are also rapidly increasing, according to the measurement technology. (patentgenius.com)
  • Luminescent materials for biomedical applications: the example of nanothermometers. (sbpmat.org.br)
  • This level of luminosity is achieved by applying micro tubes containing luminescent 3H gas with light intensity more than 100 times effective than conventional luminous paints. (watchalyzer.com)
  • The blue light excites the luminescent material. (ferret.com.au)
  • As the luminescent material relaxes it emits red light. (ferret.com.au)
  • Under a strong quantum measurement, the motion of an oscillator is disturbed by the measurement backaction, as required by the Heisenberg uncertainty principle. (bioportfolio.com)
  • Cierpka C, Kähler CJ (2012) Particle imaging techniques for volumetric three-component (3D3C) velocity measurements in microfluidics. (springer.com)