A huge subclass of mostly marine CRUSTACEA, containing over 14,000 species. The 10 orders comprise both planktonic and benthic organisms, and include both free-living and parasitic forms. Planktonic copepods form the principle link between PHYTOPLANKTON and the higher trophic levels of the marine food chains.
Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.
Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.
Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.
The family Lampyidae, which are bioluminescent BEETLES. They contain FIREFLY LUCIFERIN and LUCIFERASES. Oxidation of firefly luciferin results in luminescence.
A genus of bioluminescent marine invertebrates in the family Renillidae, order Pennatulacea, class ANTHOZOA. It contains Renilla LUCIFERASE which oxidizes coelenterazine resulting in LUMINESCENCE.
Luciferases from FIREFLIES, usually Photinus, that oxidizes FIREFLY LUCIFERIN to cause emission of PHOTONS.
A form of psychotherapy with emphasis on the interplay of organism and environment. Basic to this therapy is the development of awareness and maturity, as well as self-confidence.
Emission of LIGHT when ELECTRONS return to the electronic ground state from an excited state and lose the energy as PHOTONS. It is sometimes called cool light in contrast to INCANDESCENCE. LUMINESCENT MEASUREMENTS take advantage of this type of light emitted from LUMINESCENT AGENTS.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
The application of genetic analyses and MOLECULAR DIAGNOSTIC TECHNIQUES to legal matters and crime analysis.
A genus of very large, epibenthic SEA CUCUMBERS in the family Stichopodidae, commercially harvested in Southeast Asia for food.
The principles of proper conduct concerning the rights and duties of the professional, relations with patients or consumers and fellow practitioners, as well as actions of the professional and interpersonal relations with patient or consumer families. (From Stedman, 25th ed)
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
Systematic statements of principles or rules of appropriate professional conduct, usually established by professional societies.
Legally authorized corporations owned and managed by one or more professionals (medical, dental, legal) in which the income is ascribed primarily to the professional activities of the owners or stockholders.
A discipline concerned with studying biological phenomena in terms of the chemical and physical interactions of molecules.
A benzothaizole which is oxidized by LUCIFERASES, FIREFLY to cause emission of light (LUMINESCENCE).
INSECTS of the order Coleoptera, containing over 350,000 species in 150 families. They possess hard bodies and their mouthparts are adapted for chewing.
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Ribonucleic acid that makes up the genetic material of viruses.
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
Detection and counting of scintillations produced in a fluorescent material by ionizing radiation.
Immature ERYTHROCYTES. In humans, these are ERYTHROID CELLS that have just undergone extrusion of their CELL NUCLEUS. They still contain some organelles that gradually decrease in number as the cells mature. RIBOSOMES are last to disappear. Certain staining techniques cause components of the ribosomes to precipitate into characteristic "reticulum" (not the same as the ENDOPLASMIC RETICULUM), hence the name reticulocytes.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
A publication issued at stated, more or less regular, intervals.
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
Antibodies reactive with HIV ANTIGENS.
A large subphylum of mostly marine ARTHROPODS containing over 42,000 species. They include familiar arthropods such as lobsters (NEPHROPIDAE), crabs (BRACHYURA), shrimp (PENAEIDAE), and barnacles (THORACICA).
A saturated 12-carbon fatty alcohol obtained from coconut oil fatty acids. It has a floral odor and is used in detergents, lubricating oils, and pharmaceuticals. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
The rate dynamics in chemical or physical systems.
The scientific disciplines concerned with the embryology, anatomy, physiology, biochemistry, pharmacology, etc., of the nervous system.
The full collection of microbes (bacteria, fungi, virus, etc.) that naturally exist within a particular biological niche such as an organism, soil, a body of water, etc.
Decrease in existing BODY WEIGHT.
A collective genome representative of the many organisms, primarily microorganisms, existing in a community.
Generally refers to the digestive structures stretching from the MOUTH to ANUS, but does not include the accessory glandular organs (LIVER; BILIARY TRACT; PANCREAS).
The act or practice of calling public attention to a product, service, need, etc., especially by paid announcements in newspapers, magazines, on radio, or on television. (Random House Unabridged Dictionary, 2d ed)
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.
An ethylmercury-sulfidobenzoate that has been used as a preservative in VACCINES; ANTIVENINS; and OINTMENTS. It was formerly used as a topical antiseptic. It degrades to ethylmercury and thiosalicylate.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Luciferases from RENILLA that oxidizes certain LUMINESCENT AGENTS to cause emission of PHOTONS.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.

In vivo chaperone activity of heat shock protein 70 and thermotolerance. (1/8235)

Heat shock protein 70 (Hsp70) is thought to play a critical role in the thermotolerance of mammalian cells, presumably due to its chaperone activity. We examined the chaperone activity and cellular heat resistance of a clonal cell line in which overexpression of Hsp70 was transiently induced by means of the tetracycline-regulated gene expression system. This single-cell-line approach circumvents problems associated with clonal variation and indirect effects resulting from constitutive overexpression of Hsp70. The in vivo chaperone function of Hsp70 was quantitatively investigated by using firefly luciferase as a reporter protein. Chaperone activity was found to strictly correlate to the level of Hsp70 expression. In addition, we observed an Hsp70 concentration dependent increase in the cellular heat resistance. In order to study the contribution of the Hsp70 chaperone activity, heat resistance of cells that expressed tetracycline-regulated Hsp70 was compared to thermotolerant cells expressing the same level of Hsp70 plus all of the other heat shock proteins. Overexpression of Hsp70 alone was sufficient to induce a similar recovery of cytoplasmic luciferase activity, as does expression of all Hsps in thermotolerant cells. However, when the luciferase reporter protein was directed to the nucleus, expression of Hsp70 alone was not sufficient to yield the level of recovery observed in thermotolerant cells. In addition, cells expressing the same level of Hsp70 found in heat-induced thermotolerant cells containing additional Hsps showed increased resistance to thermal killing but were more sensitive than thermotolerant cells. These results suggest that the inducible form of Hsp70 contributes to the stress-tolerant state by increasing the chaperone activity in the cytoplasm. However, its expression alone is apparently insufficient for protection of other subcellular compartments to yield clonal heat resistance to the level observed in thermotolerant cells.  (+info)

Transcriptional regulation and induction of apoptosis: implications for the use of monomeric p53 variants in gene therapy. (2/8235)

The p53 tumour suppressor protein is a transcriptional activator, which can induce cell cycle arrest and apoptosis. p53 Gene mutations occur in more than 50% of all human tumours. Reintroduction of wild-type p53 but also of oligomerisation-independent p53 variants into tumour cells by gene transfer methods has been considered. We have investigated the biological properties of two carboxy-terminal deletion mutants of p53, p53 delta 300 (comprising amino acids 1-300) and p53 delta 326 (amino acids 1-326), to evaluate their potential deployment in gene therapy. Transactivation was measured in transiently transfected HeLa and SKBR3 cells. Both monomeric variants showed reduced activities compared with wild-type p53. Individual promoters were differently affected. In contrast to wild-type p53, monomeric variants were not able to induce apoptosis. We also provided wild-type p53 and p53 delta 326 with tetracycline-regulated promoters and stably introduced these constructs into Saos2 and SKBR3 cells. Upon induction, wild-type p53 expressing cells, but not p53 delta 326 expressing cells underwent apoptosis. Consistently, only wild-type p53 expressing cells accumulated p21/waf1/cip1 mRNA and protein and showed increased bax, Gadd45 and mdm2 mRNA. Neither wild-type p53 nor p53 delta 326 repressed the transcription of the IGF-1R gene in these cell lines. We conclude that the transactivation potential of monomeric, carboxy-terminally truncated p53 is not sufficient to cause induction of the endogenous target genes which trigger apoptosis.  (+info)

A strategy for enhancing the transcriptional activity of weak cell type-specific promoters. (3/8235)

Cell type- and tissue-specific promoters play an important role in the development of site-selective vectors for gene therapy. A large number of highly specific promoters has been described, but their applicability is often hampered by their inefficient transcriptional activity. In this study, we describe a new strategy for enhancing the activity of weak promoters without loss of specificity. The basic principle of this strategy is to establish a positive feedback loop which is initiated by transcription from a cell type-specific promoter. This was achieved by using a cell type-specific promoter to drive the simultaneous expression of the desired effector/reporter gene product and a strong artificial transcriptional activator which stimulates transcription through appropriate binding sites in the promoter. Using a VP16-LexA chimeric transcription factor, we show that this approach leads to a 14- to > 100-fold enhancement of both the endothelial cell-specific von Willebrand factor promoter and the gastrointestinal-specific sucrase-isomaltase promoter while maintaining approximately 30- to > 100-fold cell type specificity.  (+info)

Ligand substitution of receptor targeted DNA complexes affects gene transfer into hepatoma cells. (4/8235)

We have targeted the serpin enzyme complex receptor for gene transfer in human hepatoma cell lines using peptides < 30 amino acids in length which contain the five amino acid recognition sequence for this receptor, coupled to poly K of average chain length 100 K, using the heterobifunctional coupling reagent sulfo-LC SPDP. The number of sulfo-LC SPDP modified poly-L-lysine residues, as well as the degree of peptide substitution was assessed by nuclear magnetic resonance spectroscopy. Conjugates were prepared in which 3.5%, 7.8% or 26% of the lysine residues contained the sulfo-LC SPDP moiety. Each of these conjugates was then coupled with ligand peptides so that one in 370, one in 1039, or one in 5882 lysines were substituted with receptor ligand. Electron microscopy and atomic force microscopy were used to assess complex structure and size. HuH7 human hepatoma cells were transfected with complexes of these conjugates with the plasmid pGL3 and luciferase expression measured 2 to 16 days after treatment. All the protein conjugates in which 26% of the K residues were modified with sulfo-LC SPDP were poor gene transfer reagents. Complexes containing less substituted poly K, averaged 17 +/- 0.5 nm in diameter and gave peak transgene expression of 3-4 x 10(6) ILU/mg which persisted (> 7 x 10(5) ILU) at 16 days. Of these, more substituted polymers condensed DNA into complexes averaging 20 +/- 0.7 nm in diameter and gave five-fold less luciferase than complexes containing less substituted conjugates. As few as eight to 11 ligands per complex are optimal for DNA delivery via the SEC receptor. The extent of substitution of receptor-mediated gene transfer complexes affects the size of the complexes, as well as the intensity and duration of transgene expression. These observations may permit tailoring of complex construction for the usage required.  (+info)

Cadmium-mediated activation of the metal response element in human neuroblastoma cells lacking functional metal response element-binding transcription factor-1. (5/8235)

Metal response element-binding transcription factor-1 (MTF-1) binds specifically to metal response elements (MREs) and transactivates metallothionein (MT) gene expression in response to zinc and cadmium. This investigation contrasts the mechanism of mouse MT gene (mMT-I) promoter activation by cadmium and zinc in IMR-32 human neuroblastoma cells to determine whether MTF-1 binding to the MRE is necessary for activation by these metals. Cadmium activated a mMT-1 promoter (-150 base pairs) luciferase reporter 20-25-fold through a MRE-dependent mechanism. In contrast, zinc had little effect on the mMT-1 luciferase reporter. IMR-32 cells lacked MRE binding activity, and treatment with zinc in vitro or in vivo did not generate a MTF-1. MRE complex, suggesting that IMR-32 cells lack functional MTF-1. Overexpression of mMTF-1 regenerated a zinc-mediated induction of the MRE without affecting cadmium activation. Because no other transition metals tested activated the MRE, this effect appeared to be cadmium-specific. These data demonstrate that in IMR-32 human neuroblastoma cells, zinc and cadmium can use independent mechanisms for activation of the mMT-I promoter and cadmium-mediated MRE activation is independent of MTF-1 and zinc.  (+info)

Gene expression and chromatin organization during mouse oocyte growth. (6/8235)

Mouse oocytes can be classified according to their chromatin organization and the presence [surrounded nucleolus (SN) oocytes] or absence [nonsurrounded nucleolus (NSN) oocytes] of a ring of Hoechst-positive chromatin around the nucleolus. Following fertilization only SN oocytes are able to develop beyond the two-cell stage. These studies indicate a correlation between SN and NSN chromatin organization and the developmental competence of the female gamete, which may depend on gene expression. In the present study, we have used the HSP70.1Luc transgene (murine HSP70.1 promoter + reporter gene firefly luciferase) to analyze gene expression in oocytes isolated from ovaries of 2-day- to 13-week-old females. Luciferase was assayed on oocytes after classification as SN or NSN type. Our data show that SN oocytes always exhibit a higher level of luciferase activity, demonstrating a higher gene expression in this category. Only after meiotic resumption, metaphase II oocytes derived from NSN or SN oocytes acquire the same level of transgene expression. We suggest that the limited availability of transcripts and corresponding proteins, excluded from the cytoplasm until GVBD in NSN oocytes, could explain why these oocytes have a lower ability to sustain embryonic development beyond the two-cell stage at which major zygotic transcription occurs. With this study we have furthered our knowledge of epigenetic regulation of gene expression in oogenesis.  (+info)

UV-A-induced decrease in nuclear factor-kappaB activity in human keratinocytes. (7/8235)

Previous reports have demonstrated an increase in nuclear factor-kappaB (NF-kappaB) activity in response to UV radiation. These studies have essentially focused on the DNA-damaging fraction of solar UV radiation (UV-B and UV-C). In contrast, the effects of UV-A radiation (320-400 nm) on NF-kappaB are not well known. In this study, we present evidence that UV-A radiation induces a marked decrease in NF-kappaB DNA-binding activity in NCTC 2544 human keratinocytes. In addition, NCTC 2544 keratinocytes pretreated with UV-A fail to respond to NF-kappaB inducers. Moreover, UV-A radiation induces a decrease in NF-kappaB-driven luciferase reporter gene expression in NCTC 2544 keratinocytes. The expression of the gene encoding IkappaBalpha (IkappaB is the NF-kappaB inhibitor), which is closely associated with NF-kappaB activity, is also reduced (3-fold) upon UV-A treatment. Our results indicate that the UV-A-induced decrease in NF-kappaB DNA-binding activity is associated with a decrease in the levels of the p50 and p65 protein subunits. This is the first evidence that an oxidative stress, such as UV-A radiation, may induce a specific decrease in NF-kappaB activity in mammalian cells, probably through degradation of NF-kappaB protein subunits. These findings suggest that UV-A could modulate the NF-kappaB-dependent gene expression.  (+info)

Modulation of human airway smooth muscle proliferation by type 3 phosphodiesterase inhibition. (8/8235)

Elevation in cell cAMP content can inhibit mitogenic signaling in cultured human airway smooth muscle (HASM) cells. We studied the effects of the type 3-selective phosphodiesterase inhibitor siguazodan, the type 4-selective phosphodiesterase inhibitor rolipram, and the nonselective inhibitor 3-isobutyl-1-methylxanthine (IBMX) on proliferation of cultured HASM cells. At concentrations selective for the type 3 phosphodiesterase isoform, siguazodan inhibited both [3H]thymidine incorporation (IC50 2 microM) and the increase in cell number (10 microM; 64% reduction) induced by platelet-derived growth factor-BB (20 ng/ml). These effects were mimicked by IBMX. At concentrations selective for type 4 phosphodiesterase inhibition, rolipram was without effect. A 20-min exposure to siguazodan and rolipram did not increase whole cell cAMP levels. However, in HASM cells transfected with a cAMP-responsive luciferase reporter (p6CRE/Luc), increases in cAMP-driven luciferase expression were seen with siguazodan (3.9-fold) and IBMX (16.5-fold). These data suggest that inhibition of the type 3 phosphodiesterase isoform present in airway smooth muscle results in inhibition of mitogenic signaling, possibly through an increase in cAMP-driven gene expression.  (+info)

PML is required for p73 transcriptional and proapoptotic activity. (A) A bax promoter-driven luciferase reporter plasmid (bax-Luc) alone or in combination with
There was a positive correlation between CEA levels and CA 15-3 levels and patient prognosis. A high specificity for B-cells was confirmed by assaying for luciferase reporter gene expression from a plasmid containing an analogous combination of immunoglobulin heavy chain regulatory ...
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
Both the luciferase gene and a human housekeeping gene ACTB (both expressed only in human cells) were present only in the injected kidney tissue, as confirmed by the absence of the both housekeeping ACTB and luciferase gene in non-injected kidneys (Figure 3D ...
Thermo Scientific™ Gaussia Luciferase Reporter Assay Vectors pMCS-Gaussia Luc Vector Thermo Scientific™ Gaussia Luciferase Reporter Assay Vectors...
Thermo Scientific™ Pierce™ Gaussia Luciferase Flash Assay Kit 100-rxn kit Thermo Scientific™ Pierce™ Gaussia Luciferase Flash Assay...
Gaussia Luciferase (GLuc) reporter gene offers bright bioluminescence, either as a standalone expression monitor or as a fusion partner with other protein.
The half-life of GLuc remains unclear. All GLuc-expressing vectors available for mammalian expression at NEB harbor the humanized codons for GLuc in which the secreted GLuc has activity similar to that of the native protein. Once GLuc is secreted into the culture medium, it is very stable. For example, ~20% of the GLuc activity was detectable after incubating a GLuc-containing sample at 99˚C for 15 minutes ...
TY - GEN. T1 - Measurement of (anti-)oestrogenic potency in complex mixtures using a novel stably transfected luciferase reporter gene assay in the human T47D breast cancer cell. AU - Legler, J.. AU - van den Brink, C.. AU - Brouwer, A.. AU - van der Saag, P.. AU - Vethaak, A.D.. AU - Murk, A.J.. AU - van der Burg, B.. PY - 2000. Y1 - 2000. M3 - Conference paper. SP - 104. EP - 108. BT - Endocrine Disrupting Compounds : Wildlife and human health risks, The Hague 1998. CY - The Hague. ER - ...
The original aim of this thesis was to utilise Vibrio harveyi luciferase as a reporter of the expression of cell division genes during the cell cycle. Several plasmids expressing luxAB genes from ftsZ promoters were constructed. To achieve maximal luciferase expression, the ribosome binding site in front of the luxA gene was improved, which led to increased expression of luciferase. The level of expression of the improved luciferase reporter from plasmids was sufficiently high to be detected in single cells, although not high enough to be used in lower copy number constructs. However, luciferase activity showed significant fluctuations that did not appear to be linked to cell cycle events. These fluctuations made the detection of any cell cycle related changes in luciferase expression impossible. Another direction of this thesis is represented by the studies on the topology of the cell shape determining RodA protein. The ampicillin resistance levels were measured in 52 fusions with the topology ...
The pGL3 Luciferase Reporter Vectors provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors. The backbone of the pGL2 Luciferase Reporter Vectors was redesigned for the pGL3 Vectors for increased expression, and contains a modified coding region for firefly (Photinus pyralis) luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, the Luciferase Reporter Vectors contain numerous features aiding in the structural characterization of the putative regulatory sequences under investigation.. ...
p21WAF1/CIP1 plays a major role in the induction of G1 arrest following DNA damage. Although p21WAF1/CIP1 expression is regulated by the tumor suppressor p53, induction of p21WAF1/CIP1 expression through p53-independent pathways has been described in numerous cell types. In this report, we describe the mechanism by which the retinoid 6-[3-(1-adamantyl)-4-hydroxyphenyl]-2-naphthalene carboxylic acid (CD437) induces p21WAF1/CIP1 in breast carcinoma cells possessing either a wild-type (MCF-7 cells) or mutated (MDA-MB-468 cells) p53. Exposure of MDA-MB-468 cells to this retinoid results in an approximately 10-fold increase in p21WAF1/CIP1 mRNA levels, whereas less than a 2-fold increase in p21WAF1/CIP1 gene transcription was observed as indicated by transient transfection experiments utilizing a p21WAF1/CIP1 promoter firefly luciferase reporter gene construct and nuclear run-off studies. We found similar results in the MCF-7 cells (Z-M. Shao et al., Oncogene, 11: 493-504, 1995). We have now found ...
To confirm the functionality of LODER-driven siRNA in vivo, we tested the ability of a LODER containing siRNA targeting the luciferase gene, siLuc LODER, to reduce luciferase expression in normal and tumor tissues constitutively expressing the luciferase gene. We therefore implanted empty or siLuc LODERs into the livers of transgenic mice expressing the luciferase gene (MUP-Luc) in liver cells (19). In vivo imaging results showed that siLuc LODERs led to a significant decrease in luciferase levels compared with empty LODERs (Fig. S5A). Next, LODERs carrying siLuc or siGFP were implanted into CT26 cell-derived s.c. synograft tumors. Three days after implantation, measurements of luciferase activity in vivo revealed that siLuc released from the LODERs inhibited luciferase expression (Fig. S5B). This decrease in luciferase activity was not correlated to nonspecific effects on tumor growth, as tumor weights were similar within both groups (Fig. S5C). Together these results show that siRNA is ...
Luciferase Reporter Gene Assay Kit - Information The Luciferase Reporter Gene Assay is based on the quantitation of luciferase expression in mammalian, yeast or E. coil cells, using luciferin and ATP as substrates. The reaction results in light ...
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In this application note, we measure luciferase expression in CHO-K1 cells using the SpectraMax Glo Steady-Luc Reporter Assay Kit, which affords long-lasting luminescence signals.
Luciferase assay products include reporter assay reagents, a comprehensive range of reporter vectors, luciferase-expressing cell lines and low-toxicity transfection reagents to ensure successful delivery of reporter vectors to the cell line of your choice.
Material and Methods: miR-150 expression was quantified by qRT-PCR in two MIBC cell lines (5637 and T24). After successful miR-150 inhibition by transfection, MTS and transwell assays were used to assess the MIBCs cisplatin sensitivity and cell invasiveness, respectively. The TargetScan database and a luciferase reporter system were used to identify whether the programmed cell death 4 protein (PDCD4) is a direct target of miR-150 in MIBC cells ...
The LightSwitch 3´UTR Reporter Collection from Active Motif include over 12,000 3´UTRs available as transfection-ready luciferase reporter vectors for use in reporter assays.
IL-8 is a direct target of miR-106a. (a) Up: potential sites in IL-8 3′UTR targeted by miR-106a. 3′UTR of IL-8 was cloned into a luciferase reporter vector.
Plasmid pGL3-NFAT luciferase from Dr. Jerry Crabtrees lab contains the insert 3x NFAT binding sequence and is published in Nature. 1992 Jun 25. 357(6380):695-7. This plasmid is available through Addgene.
Buy Luciferase recombinant protein-BAF48390 (MBS203675) product datasheet at MyBioSource, Recombinant Proteins. Application: SDS-PAGE
To protect your privacy, your account will be locked after 6 failed attempts. After that, you will need to contact Customer Service to unlock your account.. You have 4 remaining attempts.. You have 3 remaining attempts.. You have 2 remaining attempts.. You have 1 remaining attempt.. Contact Customer Service ...
The Dual Luciferase Reporter Assay allows for the sequential measurement of the activity of two different luciferases, firefly (FFL) and Renilla (RL), in a single sample. The firefly luciferase luminescence is measured first by addition of the FFL Reagent. Next, the RL Reagent is added to the same well. The RL Reagent simultaneously quenches the firefly luciferase luminescence and initiates the Renilla luciferase reaction. The light production of both reactions can be conveniently measured on a luminometer. This bioluminescent dual reporter gene assay is extremely sensitive and is especially suitable for quantifying dual luciferase expression in recombinant cells or in cell free transcription/translation reactions. Assays can be performed in tubes, cuvettes or multi-well plates. ...
Tubulointerstitial fibrosis is a progressive process affecting the kidneys, causing renal failure that can be life-threatening. Thus, renal fibrosis has become a serious concern in the ageing population; however, fibrotic development cannot be diagnosed early and assessed noninvasively in both patients and experimental animal models. Here, we found that serum amyloid A3 (Saa3) expression is a potent indicator of early renal fibrosis; we also established in vivo Saa3/C/EBPβ-promoter bioluminescence imaging as a sensitive and specific tool for early detection and visualization of tubulointerstitial fibrosis. Saa3 promoter activity is specifically upregulated in parallel with tumor necrosis factor α (TNF-α) and fibrotic marker collagen I in injured kidneys. C/EBPβ, upregulated in injured kidneys and expressed in tubular epithelial cells, is essential for the increased Saa3 promoter activity in response to TNF-α, suggesting that C/EBPβ plays a crucial role in renal fibrosis development. Our ...
Lab Reagents Human IgG antibody Laboratories manufactures the single or dual luciferase assay reagents distributed by Genprice. The Single Or Dual Luciferase Assay reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact luciferase assay. Other Single products are available in stock. Specificity: Single Category: Or Group: Dual Luciferase. Dual Luciferase information ...
BioAssay record AID 95279 submitted by ChEMBL: Cellular activity was measured by an IL-2 luciferase reporter gene assay on a Jurkat human T cell line activated by anti-CD28 and anti-TCR antibodies.
Dual luciferase assay - proper controls? - posted in Cell Biology: Hey everyone, I am trying to perform a dual luciferase reporter assay in a cell line with promoters established from another cell line. cDNA and protein of the gene are present in both cell lines. Problem: In the new cell line, the luciferase activity for my promoters of interest is below pGL3, whereas TKpGL3 is as highly active as in the other cell line. Renilla activity was equal in all probes. So I am looking for appr...
Bioluminescent Reporter plasmid to express bacterial luciferase (Lux) in Mycobacteria Background. Bioluminescence, the production of light by luciferase-catalysed reactions, is a versatile reporter technology with multiple applications both in vitro and in vivo. In vivo bioluminescence imaging (BLI) represents one of its most outstanding uses by allowing the non-invasive localisation of luciferase-expressing cells within an animal. Applied to the study of infectious diseases, BLI permits the detection of microorganisms from within living animals thus allowing the spatiotemporal study of infection in real-time in the same host. Moreover, using luciferase as a reporter of gene expression, it is possible to establish when and where a gene function is needed, shedding light on bacterial pathogenesis. Finally, BLI constitutes an easy and rapid method to test novel antimicrobial compounds in vivo.. Three main luciferin-luciferase systems have been utilised for BLI. The first system is represented by ...
0140] The in vitro transfection efficiency of complexes prepared with modified bPEI-2 polymer and luciferase reporter gene was investigated using HepG2 and SK-OV-3 cell lines. HepG2 cells were seeded onto 24-well plates at a density of 8×104 cells per 500 microliters per well for luciferase gene delivery. SK-OV-3 cells were seeded onto 24 well plates at a density of 8×104 cells per 500 microliters per well for luciferase gene delivery. After 24 hours, the plating media were replaced with fresh growth media, followed by the drop-wise addition of 50 microliters of complex solution containing 2.5 micrograms luciferase plasmid DNA at various N/P ratios. Following 4 hours of incubation, free complexes were removed by replacing the medium in each well. After a further 68 hours of incubation, the cell culture medium in each well was removed and the cells rinsed once with 0.5 mL of phosphate-buffered saline (PBS, pH 7.4). For luciferase expression assay, 0.2 mL of reporter lysis buffer was added to ...
The temporal effects of luciferase reaction luminescence have only been discussed in the context of light intensity (flash vs. glow). However, alterations in the color of the light emitted over the course of the luciferase reaction have not been reported. Here, we show a temporal change in the light color emitted during the reaction catalyzed by unmodified firefly luciferase when concentrations of one of the substrates, adenosine triphosphate (ATP), are gradually increased. The temporal color change from green to red occurs within the first few minutes of the luciferase reaction when an ATP-containing solution is either added or synthesized in situ with the aid of an autocatalytic reaction occurring simultaneously ...
Data Availability StatementThe datasets used during the present study are available from the corresponding author upon reasonable request. negative control using Lipofectamine? 3000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.). After 48 h, cells were lysed and assayed for luciferase activity using a dual luciferase reporter assay (Promega Corporation). Normalized luciferase activity was reported as luciferase activity/luciferase activity. Statistical analysis All data are presented as the mean standard deviation. SPSS 21.0 statistical software (IBM Corp., Armonk, NY, USA) was used to explore the statistical analysis. Comparisons between two groups were conducted using two-tailed Students t-test and multiple group comparisons were conducted via one-way analysis of variance with Tukeys post hoc test. P 0.05 was considered to indicate a statistically significant difference. Results miR-106b-3p is upregulated in ESCC tissues and cell lines The expression of miR-106b-3p in 50 paired ESCC ...
Alibaba.com offers 155 luminometer products. About 27% of these are testing equipment, 25% are other measuring & analysing instruments, and 6% are other optics instruments. A wide variety of luminometer options are available to you,
Title:Effects of APC De-Targeting and GAr Modification on the Duration of Luciferase Expression from Plasmid DNA Delivered to Skeletal Muscle. VOLUME: 15 ISSUE: 1. Author(s):Maria C. Subang, Rewas Fatah, Ying Wu, Drew Hannaman, Jason Rice, Claire F. Evans, Yuti Chernajovsky and David Gould. Affiliation:Bone & Joint Research Unit, Queen Mary University of London, William Harvey Research Institute, Charterhouse Square, London EC1M 6BQ, UK.. Keywords:Gene therapy, luciferase, microRNA, plasmid DNA, skeletal muscle, tissue-specific promoter, transgene immunogenicity.. Abstract:Immune responses to expressed foreign transgenes continue to hamper progress of gene therapy development. Translated foreign proteins with intracellular location are generally less accessible to the immune system, nevertheless they can be presented to the immune system through both MHC Class I and Class II pathways. When the foreign protein luciferase was expressed following intramuscular delivery of plasmid DNA in outbred ...
a,b, Western blotting analysis of miR-20a target genes in response to increasing doses of miR-20a mimic (a) or antagomir (b). c,d, RT-qPCR analysis of miR-20a levels in HeLa cells transfected with miR-20a mimic (c) or antagomir (d). e,f, RT-qPCR analysis of DAPK3 mRNA levels in HeLa cells transfected with miR-20a mimic (e) or antagomir (f). g, Luciferase reporter constructs containing the miR-20a MRE from DAPK3 (native) in Renilla 5 UTR or mutated to restore base-pairing in the 5 end (seed) coupled with progressive mismatches (3MM+seed) in the 3 end. h,i, Results of the luciferase reporter assays (h) and quantification of luciferase mRNA (i). j,k, mRNA levels of luciferase reporters containing individual MREs from 4 indicated genes that function in both CDS and 3UTR (j) or those that function only in CDS (k), as shown in main Fig. 2e,f. l,m, Luciferase activities from the reporters containing individual MREs from 4 indicated genes that function only in CDS in response to co-transfected ...
Neutralizing anti-drug antibodies (NAbs) against therapeutic interferon beta (IFNβ) in people with multiple sclerosis (MS) are measured with cell-based bioassays. The aim of this study was to redevelop and validate two luciferase reporter-gene bioassays, LUC and iLite, using a cut-point approach to …
Cells transfected with 8 pmol of luc2-encoding IVT RNA were harvested at different time points to measure luminescence from 1 × 104 cells (Bright-Glo Luciferase Assay Kit for 96-well plates (Promega)). Results are the mean ± SD luminescence. Percent luminescence is relative to the highest luminescence signal obtained in each experiment. (c) Viability, reporter gene expression of iDCs and mDCs after eGFP and luc2 electroporation and phenotype after electroporation are depicted in descending order, respectively. iDCs (left panel) and mDCs (right panel) of 2 different donors were transfected with 10 μg eGFP- or luc2-encoding IVT RNA. Negative controls: cells electroporated without RNA (mock) and unelectroporated (no ep) cells. Cells were harvested at different time points. Viability and HLA-DR, CD83, and eGFP expression levels were determined by flow cytometry. Luciferase activity of 1 × 104 viable cells was measured by luminescence in triplicate ...
Supernatants from three individual wells per experimental condition were collected in the indicated time points and assayed for the IL-8 concentration using a human being IL-8 enzyme-linked immunosorbent assay (ELISA) kit according to the instructions of the manufacturer (BioLegend). et al. This content is distributed under the terms of the Creative Commons Attribution 4.0 International license. FIG?S2? Multiple strains induce TIFA-dependent signaling in epithelial cells. (A) Control, KO#1 AGS cells (DKO) were cocultured with the indicated strains (MOI = 10), and IL-8 concentration in the supernatant were measured by ELISA at 6 and 24?h. (B) NF-B luciferase activity in wild-type or strains (lysate normalized using OD600 measurements). NF-B luciferase transmission was normalized to transmission from cotransfected luciferase plasmid, and data are displayed as normalized collapse changes from mock-treated BQ-788 samples. (A and B) Data are representative of results from two self-employed ...
Label License, Gaussia luciferase, material transfer license, US Patent, 6232107, 6436682, 6780974, 7045599, 7109315, 7238497, HTS, high throughput screening
The recombinant cell line used in this assay (H1L6.1c2) was generated by stably transfecting the plasmid pGudLuc6.1 into mouse hepatoma (Hepa1c1c7) cells. The pGudLuc6.1 plasmid contains the CYP1A1 dioxin-responsive domain (inclusive of four DREs) upstream of the firefly luciferase gene ...
A paper published on October 2 in the Journal of Virology describes an exciting development in the world of influenza research-the construction of a luciferase reporter virus that does not affect virulence and can be used to track development and spread of infection in mice.
When I joined the lab team in May I was familiar with lab equipment and testing but I wasnt familiar with luminometers and ATP. I had heard of ATP in my
Our comprehensive collection contains more than 12,000 endogenous human 3′UTR reporter GoClone® constructs that are transfection-ready with no DNA preparation required. Our reporters contain a novel luciferase gene for industry-leading sensitivity ...
Our comprehensive collection contains more than 12,000 endogenous human 3′UTR reporter GoClone® constructs that are transfection-ready with no DNA preparation required. Our reporters contain a novel luciferase gene for industry-leading sensitivity ...
We developed a Gaussia luciferase (Gluc) reporter replicon of West Nile virus (WNV) and used it to quantify viral translation and RNA replication. The advantage of the Gluc replicon is that Gaussia luciferase is secreted into the culture medium from cells transfected with Gluc replicon RNA, and the medium can be assayed directly for luciferase activity. Using a known Flavivirus inhibitor (NITD008), we demonstrated that the Gluc-WNV replicon could be used for antiviral screening. The Gluc-WNV-Rep will be useful for research in antiviral drug development programs, as well as for studying viral replication and pathogenesis of WNV.
Candida albicans is a major fungal pathogen causing life-threatening diseases in immuno-compromised patients. The efficacy of current drugs to combat C. albicans infections is limited, as these infections have a 40-60% mortality rate. There is a real need for novel therapeutic approaches, but such advances require a detailed knowledge of C. albicans and its in vivo pathogenesis. Additionally, any novel antifungal drugs against C. albicans infections will need to be tested for their in vivo efficacy over time. Fungal pathogenesis and drug-mediated resolution studies can both be evaluated using non-invasive in vivo imaging technologies. In the work presented here, we used a codon-optimized firefly luciferase reporter system for detecting C. albicans in mice. We adapted the firefly luciferase in order to improve its maximum emission intensity in the red light range (600-700 nm) as well as to improve its thermostability in mice. All non-invasive in vivo imaging of experimental animals was performed with a
3781 In this study, we examined transfection efficiency of naked plasmid DNA using microbubbles (Optison) with ultrasound in vitro and in vivo experiments as the feasibility of a novel nonviral vector system that transfer naked plasmid DNA into a melanoma. First, we tested the feasibility of ultrasound-mediated transfection of naked plasmid DNA in mouse melanoma B16F1 cell lines. Luciferase plasmid mixed with or without Optison was transfected into cultured mouse melanoma cells using ultrasound (1MHz; 0.4 W2) for 1 sec. Interestingly, luciferase activity was dramatically increased in cells treated with Optison, while few luciferase activity could be detected without Optison (P,0.05). We then transfected luciferase plasmid mixed with Optison by means of therapeutic ultrasound into mouse melanoma tumor. Twenty four hours after transfection, luciferase activity, transfected with Optison and ultrasound, was also higher than that of plasmid alone. Finally, we examined the feasibility of therapeutic ...
The bacterial luciferase gene cassette (lux) is unique among bioluminescent bioreporter systems due to its ability to synthesize and/or scavenge all of the substrate compounds required for its production of light. As a result, the lux system has the unique ability to autonomously produce a luminescent signal, either continuously or in response to the presence of a specific trigger, across a wide array of organismal hosts. While originally employed extensively as a bacterial bioreporter system for the detection of specific chemical signals in environmental samples, the use of lux as a bioreporter technology has continuously expanded over the last 30 years to include expression in eukaryotic cells such as Saccharomyces cerevisiae and even human cell lines as well. Under these conditions, the lux system has been developed for use as a biomedical detection tool for toxicity screening and visualization of tumors in small animal models. As the technologies for lux signal detection continue to improve, it is
FIG 12 HRV16 infection does not block secretion of the Gaussia luciferase reporter. HeLa Ohio cells stably expressing the naturally secreted Gaussia luciferase (HeLa-Gluc) were infected with HRV16 (red squares) at an MOI of 20 for 1 h, followed by culture for the indicated periods of time up to 7 h postinfection (Time hpi). As controls, HeLa-Gluc cells were uninfected (blue circles) or uninfected but treated with BFA (green triangles). The cell culture medium was removed and replaced every 30 min, and the cells were harvested at 1-h intervals. The Gluc activity then was measured in the culture media and cell lysates. The cumulative secretion over time is calculated by adding each successive 30-min culture media sample to the previous total. Panel A shows Gluc activity in the cell lysate, and panel B shows secreted Gluc activity in the cell culture medium. Each point represents the mean relative light units (RLU) (± standard deviations) as a measure of Gluc activity from triplicate assay points ...
Chromosomal aberrations of BCL11A at 2p16.1 have been reported in a variety of B-cell malignancies and its deficiency in mice leads to a profound block in B-cell development. Alternative pre-mRNA splicing of BCL11A produces multiple isoforms sharing a common N-terminus. The most abundant isoform we have identified in human lymphoid samples is BCL11A-XL, the longest transcript produced at this locus, and here we report the conservation of this major isoform and its functional characterization. We show that BCL11A-XL is a DNA-sequence-specific transcriptional repressor that associates with itself and with other BCL11A isoforms, as well as with the BCL6 proto-oncogene. Western blot data for BCL11A-XL expression coupled with data previously published for BCL6 indicates that these genes are expressed abundantly in germinal-center-derived B cells but that expression is extinguished upon terminal differentiation to the plasma cell stage. Although BCL11A-XL/BCL6 interaction can modulate BCL6 DNA binding in
Continuous increase in the number and the variety of anthropogenic sources of electromagnetic radiation causes a high interest in studying the effects ultrahigh frequency on living organisms. In the present research inflence of UHF EMR (15 W, 2.45 GHz) for 5 and 15 min on morphological and genetic peculiarities of Photobacterium phosphoreum colonies was studied. It has been revealed that UHF EMR affected colony growth parameters, induced transcriptional activity of luciferase encoding gene expression and that the effect was depended on exposure duration. The subsequent cultivation of bacteria during a two week period after treatment showed maintaining of the increased luxb mRNA level in irradiated colonies. Opposite bacterial stress responses were detected to UHF EMR and elevated temperature treatments that assumed UHF EMR comprised of not only thermal but specifi component of non-thermal nature.. The Opened International University of Human Development Ukraine ...
The attractiveness of secreted luciferases as reporters is a strong stimulus for the investigation and exploitation of new bioluminescent systems. Metridia longa is a small luminous marine copepod (Fig. 3D). The bioluminescence originates as a secretion from epidermal glands located in the head part and abdomen in response to mechanical, electrical, or chemical stimuli. Bioluminescence in Metridia longa may well serve as a defense mechanism against predators; the release of a luminous bolus from the animal is accompanied by rapid swimming that displaces the copepod away from its glowing phantom. This luciferase emits light at a peak of 480 nm with a broad emission spectrum extending to 600 nm. Gaussia luciferase has been cloned, overexpressed in bacteria, and used as a sensitive analytical reporter for hybridization assays and monitoring of cellular expression in culture and in vivo (Tannous et al. 2005). 7 Coelenterazine Dependent Luciferases 9 Fig. 3 Origins of Glow-light reporter genes used ...
The pCMV-GLuc 2 Control Plasmid is a mammalian expression vector that encodes the secreted luciferase from the copepod Gaussia princeps as a reporter, under the control of the constitutive CMV (cytomegalovirus) promoter. Gaussia luciferase (GLuc) is a 19 kDa protein encoded by a humanized sequence, and it contains a native signal peptide at the N-terminus that allows it to be secreted from mammalian cells into the cell culture medium. A neomycin resistance gene under the control of an SV40 promoter allows selection for stable integration of the plasmid into the mammalian cell genome using G418.
Since GARP appears to regulate TGF-1 secretion by T cells and TGF-one is essential for the suppressive perform of Treg cells, we sought to identify mechanisms
Bringing the firefly luciferase gene from Promegas pGL4.10[luc2] vector into a BioBrick compatible form as a sensitive reporter gene. To amplify the signal of the luciferase three different sensitivity tuners are assembled before the luciferase gene. The sensitivity tuners were created in 2007 by the iGEM team from Cambridge and amplify the read-out signal. We also assembled the firefly luciferase behind three different strong constitutive promoters for gathering additional information about this reporter gene (Cambridge, 2007). You can find this BioBrick here: BBa_K389004 You can find this BioBrick with sensitivity tuner 1 here: BBa_K389401 You can find this BioBrick with sensitivity tuner 2 here: BBa_K389402 You can find this BioBrick with sensitivity tuner 3 here: BBa_K389403 You can find this BioBrick under the control of a weak constitutive promoter here: BBa_K389302 You can find this BioBrick under the control of a medium strong constitutive promoter here: BBa_K389307 You can find this ...
D-Luciferin Firefly, free acid 4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid Luciferin is a common bioluminescent reporter used for in-vivo imaging of the expression of the luc marker gene . It is the substrate for the Firefly luciferase enzyme which utilizes ATP a ...
Andrew C. Liu is the author of this article in the Journal of Visualized Experiments: Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
Background Indie luciferase reporter assays and fluorescent translocation assays have already been successfully found in medication discovery for a number of molecular targets. would work for high throughput testing and can determine small substances that hinder FOXO signaling at different amounts. Background Forkhead package O (FOXO) proteins are growing as transcriptional integrators of pathways that regulate a number of cellular procedures, including differentiation, rate of metabolism, tension response, cell routine and apoptosis [1-3]. FOXO transcription elements have been suggested to do something as em real /em tumor suppressors because of the inhibitory results on cell routine and success [4], properties mediated by their binding as monomers to consensus DNA binding sites. Their transcriptional activity is certainly governed with a network of signaling occasions, the best known of which may be the phosphorylation of FOXO proteins at three extremely conserved serine and threonine IL6ST ...
We have studied the PAC-2 cell line extensively 6,7,14,15 and so have determined a set of electroporation conditions that provide optimal transfec-tion
Plasmids. The IFN-γ firefly-luciferase construct was generated by subcloning of specific IFN-γ-specific promoter regions (chr10:118439048-118441048, mm10) into pGL4.27 (Promega). The TEAD reporter, 8xGTIIC, murine Yap, murine Taz, and dominant-negative human TEAD1 (dnTEAD) were all described previously (76).. Cell culture and luciferase assay. HEK293T cells were maintained at 37°C with 5% CO2 in DMEM supplemented with 10% FBS, 1% penicillin, and 1% glutamate and streptomycin. All transfections were completed using FuGENE 6 (Roche). Luciferase experiments included 250 ng IFN-γ firefly-luciferase reporter constructs, 80 ng Yap or Taz, 80 ng dnTEAD, and 75 ng pGL2-Basic-Renilla luciferase (Promega). All transfections maintained an equal concentration of total DNA with transfection of pCMV-Sport6 empty vector (Invitrogen, Thermo Fisher Scientific). Cellular extracts were collected 48 hours after transfection and used in a dual-luciferase assay (Promega). Firefly luciferase activity was ...
Jurkat-Lucia™ NFAT-CD16 cells are human reporter cells for the early nuclear translocation of NFAT upon antibody-dependent cellular cytotoxicity (ADCC) induction. A bioluminescent signal is produced by an NFAT-dependent Lucia luciferase reporter protein.
The cell lysates were collected for luminescence quantification using the protocol DLR-0-INJ (with 10 s integral time) of the GloMaxTM Luminometer (Promega). Idasanutlin manufacturer Ten microliter of each sample was treated with 50 μL of Luciferase Assay Reagent II to obtain the first measurement, while the second measurement was acquired upon addition of 50 μL Stop & Glo® Reagent. The ratio of the first and second luminescence readings was taken as the. level of desired plasmid activation. The Stealth siRNA (Invitrogen) designated S1, S2 or S3 were designed to target human SARM in three different domains. HEK293 cells were seeded into 24-well plates at a density of 1×105 cells/well in 0.5 mL medium, and were transfected with expression vectors and luciferase reporter genes together with siRNA for 24 h. Then the cells were harvested and divided into two halves, one for measurement of SARM mRNA level by end-point PCR and the other for luciferase assay. To examine the effect of LPS ...
Read independent reviews on Orion L Microplate Luminometer from Titertek-Berthold (Berthold Detection Systems GmbH) on SelectScience
ences derived from the estrogen responsive Complement three (C3) or Metalloproteinase 1 (MMP1) gene. The transfection efficiency was monitored by the
Plasmid pAAV:cTNT::Luciferase from Dr. William Pus lab contains the insert Firefly luciferase and is published in Circ Res. 2014 Jul 18;115(3):354-63. doi: 10.1161/CIRCRESAHA.115.303632. Epub 2014 May 15. This plasmid is available through Addgene.
Name of Products GFP and Luciferase (firefly) Expressing Human Dermal Fibroblasts-Neonatal (GFP-Luc-HDFCs-Neo) Catalogue Number UBP-0008-NeoGFP-Luc Product
Description of a method for the co-transfection of individual 3UTR luciferase reporter constructs with a miRNA mimic. This method uses the LightSwitch Luciferase Assay System and the protocol is efficient, reproducible, and amenable to high-throughput analysis.
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I am interested in obtaining luminescence in E. coli in-vivo and would like information on plasmids including sequence and restriction maps. I would like to find someone who has some expertise with luciferase and perhaps has a variety of constructs available. Thanks for your help, David Rosen ...
Luciferase Control RNA from Promega,in vitro Translation, Accessory Products,biological,biology supply,biology supplies,biology product
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ScopesMonkey is a fanfiction author that has written 113 stories for StarTrek: Deep Space Nine, StarTrek: Other, Firefly, and Sherlock.
Because the reporter enzymes themselves (e.g. firefly luciferase) can be direct targets of small molecules and confound the ... Smale, S. T. (2010-05-01). "Luciferase Assay". Cold Spring Harbor Protocols. 2010 (5): pdb.prot5421. doi:10.1101/pdb.prot5421. ... Solberg, Nina; Krauss, Stefan (2013). "Luciferase assay to study the activity of a cloned promoter DNA fragment". Gene ... "A GUS/Luciferase Fusion Reporter for Plant Gene Trapping and for Assay of Promoter Activity with Luciferin-Dependent Control of ...
Luciferase was first purified by McElroy and Green in 1955. It was later discovered that there were two subunits to luciferase ... utilizing luciferase and luciferin), there are different types of luciferin-luciferase systems. For bacterial bio-luminescence ... LuxAB codes for luciferase while luxCDE codes for a fatty-acid reductase complex that is responsible for synthesizing aldehydes ... All bacterial luciferases are approximately 80 KDa heterodimers containing two subunits: α and β. The α subunit is responsible ...
Luciferase enzymes produce bioluminescence. Squids can then emit the luminescence from the light organ. Because Euprymna ... After bacterial cells divide and increase in population, they begin expressing enzyme luciferase as a result of quorum sensing ...
These bioassays form a group of the so-called CALUX (Chemically Activated LUciferase eXpression) bioassays. These systems are ... firefly luciferase). That is an in vitro reporter gene assays detecting estrogen receptor (ER) activation. ... endogenously together with an ER-specific Luciferase construct. A version of the ER CALUX bioassay, consisting of the human ... U2OS osterosarcoma cell line transfected with both ERα and ER-specific Luciferase construct is currently under validation to be ...
These bacteria did not synthesize luciferase-and therefore did not luminesce-in freshly inoculated culture but only after the ... However, when the population reaches the threshold in the photophore (about 1011 cells/ml), transcription of luciferase is ... Nealson, Kenneth H. (1977). "Autoinduction of bacterial luciferase". Archives of Microbiology. Springer Science and Business ...
Other names in common use include bacterial luciferase, aldehyde monooxygenase, luciferase, and Vibrio fischeri luciferase. As ... Hastings JW, Presswood RP (1978). "Bacterial luciferase: FMNH2-aldehyde oxidase". Methods Enzymol. Methods in Enzymology. 53: ...
... the discovery of a flavin to be a substrate in its luciferase reaction, the determination of gene regulation of the luciferases ... that contain both the luciferase and the luciferin-binding protein. More recently, the lab found that the luciferase gene in ... In other work when he was in the McElroy lab he examined the basic biochemical mechanism of firefly luciferase and demonstrated ... controls luciferase activity by regulating turnover". J. Biol. Chem. 256 (20): 10509-10518. PMID 7197271. Nealson, K.H.; ...
The light originates by way of a photoprotein, which differs from the photogenic molecule luciferase in firefly beetles. ... and structure function relationships of insect luciferases". Cellular and Molecular Life Sciences. 59 (11): 1833-1850. doi: ...
... belonging to a group of coelenterazine luciferases. Unlike other luciferases, it has a broader substrate specificity [3,4,6] ... The proteins signal the enzyme for secretion in luminescence, catalyzed by the protein 19 kDa [3,4,7]. The luciferase has many ... This mechanism causes O.gracilirostris release a luminous, bright blue luciferase cloud [2]. The small protein subunit of OpLuc ... In enzymology, an Oplophorus-luciferin 2-monooxygenase (EC, also known as Oplophorus luciferase (referred in this ...
The glow is the result of a chemical reaction that involves luciferin, the substrate; luciferase, the enzyme that acts upon ...
16(11): p. 2350-9. Taneoka, A., et al., The construction of a glucose-sensing luciferase. Biosens Bioelectron, 2009. 25(1): p. ... Daunert makes a Ggbp-split aqueorin sensor and in 2009 Koji Sode made Ggbp-luciferase with Asp459Asn (Glc not Gal). In addition ... such as Aqueorin from symbiont in jellyfish and luciferase from symbiont in fireflies. These have been used to make glucose ...
... belonging to a group of coelenterazine luciferases. Of this group of enzymes, the luciferase from Renilla reniformis has been ... Renilla luciferase contains 311 amino acids, and is active as a nearly spherical single polypeptide chain monomer of 36 kDa, ... However, this enzyme appears to be unrelated from most other luciferases that act on coelenterazine, such as those from ... Loening AM, Fenn TD, Gambhir SS (December 2007). "Crystal structures of the luciferase and green fluorescent protein from ...
They add to the function of the luciferins whose usual light-producing reaction is catalyzed by the enzyme luciferase. The term ... Photoproteins do not display typical enzyme kinetics as seen in luciferases. Instead, when mixed with luciferin, they display ... rather than the prolonged glow that is seen for luciferases when luciferin is added. In this respect, it may appear that ... was meant to distinguish them from other light-producing proteins because these do not exhibit the usual luciferin-luciferase ...
The bacterial luciferin-luciferase system is encoded by a set of genes labelled the lux operon. In A. fischeri, five such genes ... This operon has a known gene sequence of luxCDAB(F)E, where luxA and luxB code for the protein subunits of the luciferase ... Luciferase produces blue/green light through the oxidation of reduced flavin mononucleotide and a long-chain aldehyde by ... Eberhad, A (1981). "Structural identification of autoinducer of Photobacterium fischeri luciferase". PubMed. Retrieved 2020-04- ...
"Structural identification of autoinducer of Photobacterium fischeri luciferase". Biochemistry. 20 (9): 2444-2449. doi:10.1021/ ...
Luciferase is injected just before taking the images. Intravital microscopy with multi photon excitation is a technique to ... The substrate luciferin, is oxidized to oxyluciferin in the presence of luciferase and emits light, which can be detected using ...
2008). "Novel Genetically Encoded Biosensors Using Firefly Luciferase". ACS Chem. Biol. 3 (6): 346-51. doi:10.1021/cb8000414. ...
At low cell concentrations, V. fischeri did not express the luciferase gene. However, once the cultures had reached exponential ... luciferase) only when cultures had reached a threshold population density. ... "Structural identification of autoinducer of Photobacterium fischeri luciferase". Biochemistry. 20 (9): 2444-2449. doi:10.1021/ ... growth phase, the luciferase gene was rapidly activated. This phenomenon was termed "autoinduction" because it involved a ...
This enzyme is also called Watasenia-type luciferase. Inoue S, Kakoi H, Goto, T (1976). "Squid bioluminescence. III. Isolation ...
There he worked with William D. McElroy, who had recently isolated the enzyme luciferase. Seliger used his measurement ... Wood, K.; Lam, Y.; Seliger, H.; McElroy, W. (1989). "Complementary DNA coding click beetle luciferases can elicit ...
Keller, G. A.; Gould, S.; Deluca, M.; Subramani, S. (May 1987). "Firefly luciferase is targeted to peroxisomes in mammalian ... it was described that firefly luciferase is targeted to peroxisomes in mammalian cells, allowing the discovery of the import ...
... luciferase is a kleptoprotein, obtained from their diet on bioluminescent ostracods. There are currently 11 ... Parapriacanthus fish obtain luciferase from ostracod prey". Science Advances. American Association for the Advancement of ...
The light-producing enzyme is luciferase, and is found within cells of the lantern. Luciferases require oxygen, luciferin and ... "Firefly luciferase is targeted to peroxisomes in mammalian cells". Proceedings of the National Academy of Sciences. 84: 3264- ...
Gaussia princeps is used in the production of luciferase. J. Kouwenberg & G. Boxshall (2009). T. C. Walter & G. Boxshall (ed ...
ATP powers the oxidation of luciferase through the luciferase; this reaction generates a light signal recorded as a pyrogram ...
... but obtains the luciferase enzyme within these organs from bioluminescent ostracods, including Cypridina noctiluca or Vargula ... Parapriacanthus fish obtain luciferase from ostracod prey". Science Advances. American Association for the Advancement of ...
It is the substrate of luciferase (EC, which is responsible for the characteristic yellow light emission from many ... Such differences are likely due to pH changes or differences in primary structure of the luciferase. Modification of the ... In firefly, oxidation of luciferins, which is catalyzed by luciferases, yields a peroxy compound 1,2-dioxetane. The dioxetane ... Gomi K, Kajiyama N (2001). "Oxyluciferin, a Luminescence Product of Firefly Luciferase, Is Enzymatically Regenerated into ...
Luciferase, including ReBiL (recombinase enhanced bimolecular luciferase) and Gaussia princeps luciferase. Commercial products ... Kolkhof P, Werthebach M, van de Venn A, Poschmann G, Chen L, Welte M, Stühler K, Beller M (March 2017). "A Luciferase-fragment ... 2014) Split luciferase complementation assay to detect regulated protein-protein interactions in rice protoplasts in a large- ... "Benchmarking a luciferase complementation assay for detecting protein complexes". Nature Methods. 8 (12): 990-2. doi:10.1038/ ...
Luciferase}}][{\text{other cofactors}}]}}} Oxyluciferin + light energy Instead of a luciferase, the jellyfish Aequorea victoria ... generally called luciferin and luciferase, respectively. Because these are generic names, luciferins and luciferases are often ... Conversely, luciferase enzymes vary widely and tend to be different in each species. Play media Bioluminescence occurs widely ... ISBN 978-1-134-18630-3. Koo, J.; Kim, Y.; Kim, J.; Yeom, M.; Lee, I. C.; Nam, H. G. (2007). "A GUS/Luciferase Fusion Reporter ...
Other commonly used reporters include green fluorescent protein and luciferase. Targeting sequence: Expression vectors may ...
The reaction between luciferin and oxygen is catalyzed by the enzyme luciferase. Luciferases, like luciferins, usually have ... Luciferase- An enzyme that catalyzes the reaction between oxygen and luciferin.. Luciferin- Complex carbon molecules that ... The reaction between luciferin and oxygen is catalyzed by the enzyme luciferase. Luciferases, like luciferins, usually have ... The reaction between luciferin and oxygen is catalyzed by the enzyme luciferase. Luciferases, like luciferins, usually have ...
Luciferases can be produced in the lab through genetic engineering for a number of purposes. Luciferase genes can be ... In the luciferase reaction, light is emitted when luciferase acts on the appropriate luciferin substrate. Photon emission can ... Luciferase can act as an ATP sensor protein through biotinylation. Biotinylation will immobilize luciferase on the cell-surface ... in addition to the amount of luciferase. Luciferase is a heat-sensitive protein that is used in studies on protein denaturation ...
During a reaction, luciferase has a conformational change and goes into a "closed" form with the two domains coming together to ... This shows that luciferase can differentiate between the isomers of the luciferin structure. L-luciferin is able to emit a weak ... Firefly luciferase generates light from luciferin in a multistep process. First, D-luciferin is adenylated by MgATP to form ... Firefly luciferase is the light-emitting enzyme responsible for the bioluminescence of fireflies and click beetles. The enzyme ...
Gaussia Luciferase (GLuc) reporter gene offers bright bioluminescence, either as a standalone expression monitor or as a fusion ... Gaussia Luciferase. Product Listing Application Overview Gaussia Luciferase (GLuc) is a recently discovered, naturally secreted ... NEB offer a variety of products that utilize secreted Gaussia Luciferase (GLuc) and Cypridina Luciferase (CLuc) in reporter ... Luciferase Cell Lysis Buffer. *Protein expression using the K. lactis Protein Expression Kit - Cloning a PCR fragment into ...
... David C. Rosen darosen at eis.calstate.edu Wed Jul 6 20:10:42 EST 1994 *Previous message: Ligase Chain ... I would like to find someone who has some expertise with luciferase and perhaps has a variety of constructs available. Thanks ...
An extremely sensitive reagent for rapid quantitation of firefly luciferase. Can be used for assays in single-tube luminometers ... Luciferase Assay System Technical Bulletin PDF (413 KB) - English Quick Protocols. Luciferase Assay System Quick Protocol FB026 ... Nano-Glo® Luciferase Assay System. Add-mix-measure assay for NanoLuc® Luciferase with >2 hour half-life in most applications. ... Dual-Glo® Luciferase Assay System. Consecutive detection of firefly and Renilla luciferase activities in a single sample. Two- ...
Cypridina Luciferase (CLuc) reporter genes ultrasensitive bioluminescence activity can be measured in mammalian living cells ... Cypridina Luciferase. Product Listing Application Overview Cypridina Luciferase (CLuc) is secreted from the marine ostracod, ... NEB offer a variety of products that utilize secreted Gaussia Luciferase (GLuc) and Cypridina Luciferase (CLuc) in reporter ... Activity of Gaussia (GLuc) and Cypridina (CLuc) Luciferases Expressed in CHO Cells.. Activity was determined from the ...
ATCC is bridging the gap between the in vitro and the in vivo by offering luciferase-labeled cell lines for biological ... ATCC maintains luciferase-expressing reporter cell lines derived from the most commonly used cells in molecular imaging studies ... The addition of the luciferase reporter to these cell lines increases their utility by allowing for real-time imaging of the ... These cells either constitutively or inducibly express a reporter gene (such as GFP, RFP, luciferase, or lacZ). ATCC is ...
To protect your privacy, your account will be locked after 6 failed attempts. After that, you will need to contact Customer Service to unlock your account.. You have 4 remaining attempts.. You have 3 remaining attempts.. You have 2 remaining attempts.. You have 1 remaining attempt.. Contact Customer Service ...
... biosci-request at net.bio.net biosci-request at net.bio.net Fri Jun 25 13:43:08 EST 1999 *Previous ... Does anyone have a plasmid that can be used to express firefly luciferase in S. cerevisiae? Id be interested in both ...
Lucifer means light-bringer, and luciferases are a loose family of enzymes that are used by many organisms to produce light ... Aequorin is the luciferase used by other marine animals, like squid, but GFP is not a luciferase. However, both proteins have ... luciferase (thing). See all of luciferase, there is 1 more in this node. ... Finally, probably the best known example of a luciferase is in the firefly, although it is a symbiotic bacterium that does the ...
... ,in vitro Translation, Accessory Products,biological,biology supply,biology supplies,biology ... Luciferase Luminescent Assay Kit, LucLite from PerkinElmer. 4. Luciferase Reporter Assay Kit from MBL International. 5. LabLinx ... pMIR-mU6-Luc, Mouse U6 Luciferase Control Vector from Mirus Bio Corporation. 2. pMIR-hU6-Luc, Human U6 Luciferase Control ...
An easy-to-use directory for life science and biomedical research products. Find special deals on products, order catalogs and browse product lines from suppliers of reagents, antibodies, laboratory equipment, and more.
... Dave Bates dobates at ucdavis.edu Wed Apr 6 17:53:29 EST 1994 *Previous message: ... provides luciferase as the positive control. I am wondering which of the ,assays - standard one using luminometer or ...
Measuring HIV neutralization in a luciferase reporter gene assay.. Montefiori DC1. ... assays may be used with molecularly cloned Env-pseudotyped viruses for greater reagent stability and traceability.A luciferase ...
Firefly luciferase is much brighter than the bacterial luciferase sometimes used for chemiluminescence studies. The process ... Researchers have developed a way to use firefly luciferase - which is much brighter than bacterial luciferase - as a ... They engineered BL21 and XLU102 E. coli strains to express firefly luciferase. The first strain was otherwise normal, while the ... The investigators saw a clear difference in the two cell lines and were able to map the spatial distribution of luciferase ...
William Kaelins lab contains the insert Firefly Luciferase and is published in Proc Natl Acad Sci U S A. 2006 Jan 3. 103(1): ... Luciferase-pcDNA3 was a gift from William Kaelin (Addgene plasmid # 18964 ; http://n2t.net/addgene:18964 ; RRID:Addgene_18964) ...
Plasmid 10XSTAT92E-luciferase from Dr. Norbert Perrimons lab contains the insert SOCS36E enhancer fragment and is published in ... Finally, an XhoI/XbaI fragment containing the firefly luciferase gene from the pGL3-luciferase vector (Promega) was subcloned ... 10XSTAT92E-luciferase was a gift from Norbert Perrimon (Addgene plasmid # 37393 ; http://n2t.net/addgene:37393 ; RRID:Addgene_ ... into the XhoI/XbaI sites of 10XSTAT92E-hsp70 to generate 10XSTAT92E-luciferase. ...
Thus the oxyluciferin-luciferase complex is the lightemitter. The binding is equimolar, with dissociation constant KD equal to ... Cypridina Bioluminescence: Light-Emitting Oxyluciferin-Luciferase Complex. By Osamu Shimomura, Frank H. Johnson, Takashi Masugi ... Cypridina Bioluminescence: Light-Emitting Oxyluciferin-Luciferase Complex. By Osamu Shimomura, Frank H. Johnson, Takashi Masugi ... Cypridina Bioluminescence: Light-Emitting Oxyluciferin-Luciferase Complex Message Subject. (Your Name) has forwarded a page to ...
Daily News How Gaining and Losing Weight Affects the Body Millions of measurements from 23 people who consumed extra calories every day for a month reveal changes in proteins, metabolites, and gut microbiota that accompany shifts in body mass.. ...
MiRNA-UTR etkileşimleri daha iyi anlamak için, biz, bir roman luciferase geninin ve tahlil reaktif, LightSwitch sistemi ile ... Aldred, S. F., Collins, P., Trinklein, N. Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System ...
Mouse monoclonal Firefly Luciferase antibody [Luci17]. Validated in WB and tested in Mouse, Human. Cited in 16 publication(s). ... Luciferase from the firefly and the luciferase genes can be synthesized and inserted into organisms or transfected into cells. ... The total light emission is proportional to the luciferase activity of the sample. The use of an antibody to detect luciferase ... We are using pGL3 Luciferase Reporter Vector. It has a modified coding region for firefly (Photinus pyralis) luciferase that ...
Rabbit recombinant monoclonal Renilla Luciferase antibody [EPR17792] validated for WB, Flow Cyt, ICC/IF. With 1 independent ... The Green Renilla luciferase is a 36kDa protein produced by a derivative of the wild type Renilla luciferase gene from the sea ... WB: Renilla Luciferase transfected HEK293T whole cell lysate. ICC/IF: Renilla Luciferase transfected HEK293T cells. Flow Cyt: ... cells transfected with Empty vector or Renilla Luciferase, labeling Renilla Luciferase with ab185926 at 1/1000 dilution, ...
LightSwitch luciferase परख प्रणाली पूरी तरह से अनुकूलित संवाददाता प्रणाली है कि GoClone RenSP luciferase जीन और LightSwitch ... बेहतर miRNA-UTR बातचीत को समझने के लिए, हम 3 UTR luciferase संवाददाताओं के एक संग्रह जीनोम चौड़ा एक उपन्यास luciferase जीन और ... लक्ष्यीकरण miRNA की उपस्थिति में लक्ष्य 3UTR luciferase पछाड़ना दिखा. जाने 7a द्वारा 3UTR luciferase संवाददाता गतिविधि ... luciferase संवाददाता डेटा स्थिर राज्य प्रतिलेख स्तर के उपाय करने के लिए तुलना में किया जा सकता है. Luciferase ...
... and triple mutant C62/146/164S of the Luciola mingrelicafirefly luciferase carrying... ... firefly luciferase Luciola mingrelica site-directed mutagenesis cysteine residues polyhistidine tag kinetic parameters thermal ... and triple mutant C62/146/164S of the Luciola mingrelica firefly luciferase carrying C-terminal His6-tag were obtained on the ...
Use a secreted luciferase reporter for promoter studies in bacteria, cultured cells, and transgenic plants or animals. ... Luciferase Reporter-Ready-To-Glow Secreted Luciferase Assay. The Ready-To-Glow Secreted Luciferase Assay is based on the ... Secreted luciferase assay protocol eliminates cell lysis *2-4 fold higher signal than firefly or Renilla luciferase *Single ... thereby initiating transcription of the secreted Metridia luciferase reporter gene. *The CRE Secreted Luciferase Reporter ...
Luciferase transfection controls - (Mar/24/2010 ). Hi, I am doing a luciferase reporter assay. I would like to have a control ... Yes, the usual way to go is the dual luciferase assay, at least as far as I know... I have never done this and will never do, ... would suck if only selection vector entered the cell, but luciferase did not Co-transfections are never perfect, but in your ... Im doing a Renilla luciferase assay to measure the activity of protein promoter "A", and Im transfecting with another plasmid ...
This antibody reacts with Luciferase samples. Supplied as 100 µL purified antibody (0.9 mg/ml) in 0.1M tris glycine with 20% ... Firefly luciferase Polyclonal Antibody from Invitrogen for Western Blot applications. ... Cite Firefly luciferase Polyclonal Antibody. The following antibody was used in this experiment: Firefly luciferase Polyclonal ... Protein Aliases: Firefly Luciferase; Genji firefly; Green Luciferase; Japanese firefly; Luciferin 4-monooxygenase; Photinus ...
I am planning to do a dual luciferase assay using the firefly and renilla luciferase vectors. Can someone suggest me a ratio ... I am planning to do a dual luciferase assay using the firefly and renilla luciferase vectors.. Can someone suggest me a ratio ... The Dual-Luciferase Assay chemistry will quench the firefly signal 10,000-fold, so this more has to do with maintaining more ... Dual Luciferase Assay. Started by Sank, Jan 13 2010 09:04 AM ... The reason is that luciferase and renilla constructs can have ...
Quantitative High-Throughput Luciferase Screening in Identifying CAR Modulators.. Lynch C1, Zhao J1, Wang H2, Xia M3. ... A double stable HepG2.CYP2B6.CAR cell line was used to perform a luciferase assay in agonist and antagonist mode. Varying ... Constitutive Androstane Receptor (CAR); Cytochrome P450 2B6 (CYP2B6); Luciferase; Quantitative high-throughput screening (qHTS) ...
  • Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a photoprotein. (wikipedia.org)
  • Firefly luciferase is the light-emitting enzyme responsible for the bioluminescence of fireflies and click beetles. (wikipedia.org)
  • Luciferase can function in two different pathways: a bioluminescence pathway and a CoA-ligase pathway. (wikipedia.org)
  • Firefly luciferase bioluminescence color can vary between yellow-green (λmax = 550 nm) to red (λmax = 620). (wikipedia.org)
  • Lucifer means "light-bringer", and luciferases are a loose family of enzymes that are used by many organism s to produce light - a phenomenon known as bioluminescence . (everything2.com)
  • molecular activities of luciferase for the bioluminescence reaction and for the hydrolysis of oxy-luciferin are 1600 and 2 per minute, respectively. (sciencemag.org)
  • Luciferases are different from photoproteins, which are also involved in bioluminescence . (biology-online.org)
  • Obtain bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters. (fishersci.ca)
  • The Thermo Scientific™ Pierce Cypridina Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters. (fishersci.ca)
  • The Influence of Hypoxia and pH on Bioluminescence Imaging of Luciferase-Transfected Tumor Cells and Xenografts," International Journal of Molecular Imaging , vol. 2013, Article ID 287697, 9 pages, 2013. (hindawi.com)
  • The modified firefly luciferase expressed in C. albicans (Mut2) was found to significantly increase the sensitivity of bioluminescence imaging (BLI) in systemic infections as compared to unmodified luciferase (Mut0). (frontiersin.org)
  • Firefly Luciferase Mutants Allow Substrate-Selective Bioluminescence Imaging in the Mouse Brain. (umassmed.edu)
  • Aminoluciferins extend firefly luciferase bioluminescence into the near-infrared and can be preferred substrates over D-luciferin. (umassmed.edu)
  • The effect of CoA on the characteristic light decay of the firefly luciferase catalysed bioluminescence reaction was studied. (nih.gov)
  • At least part of the light decay is due to the luciferase catalysed formation of dehydroluciferyl-adenylate (L-AMP), a by-product that results from oxidation of luciferyl-adenylate (LH2-AMP), and is a powerful inhibitor of the bioluminescence reaction (IC50 = 6 nm). (nih.gov)
  • The bioluminescence produced by luciferase, a firefly enzyme, requires three substrates: luciferin, ATP and oxygen. (springer.com)
  • We have recently demonstrated that Renilla luciferase (Rluc) is a promising bioluminescence reporter gene that can be used for noninvasive optical imaging of reporter gene expression in living mice, with the aid of a cooled charged couple device (CCD) camera. (spiedigitallibrary.org)
  • We used a low-light video imaging system to establish that luciferase bioluminescence in vivo accurately reports the temporal and spatial regulation of cab2 transcription in single seedlings. (plantcell.org)
  • Luciferase-labelled BxPC3 cells inoculated orthotopically into the pancreas were successfully followed for 5 weeks with non-invasive bioluminescence imaging by IVIS, and the results demonstrated high-quality follow-up of tumor growth compared with tumor models using non-labelled cells. (aacrjournals.org)
  • Firefly luciferase catalyzes the oxidative carboxylation of luciferin, a reaction with the highest efficiency of any known bioluminescence reaction. (promokine.info)
  • Tissue damage was much more pronounced than the luciferase expression following thioacetamide treatment when compared with tissue damage and bioluminescence of the other toxicants. (ovid.com)
  • Available in a variety of promoter-reporter combinations that enable multiple imaging modalities-fluorescence with copGFP, bioluminescence with luciferase, and positron emission tomography (PET) with thymidine kinase (TK)-you can select from lentivector or minicircle technologies for both integrated and episomal expression. (systembio.com)
  • In the present study, to develop a new cancer cell line that is applicable to bioluminescence and fluorescence imaging, we transfected the expression vector of a green fluorescent protein ZsGreen1 into a metastatic cell line 5a-D-Luc, which is a subclone of the MDA-MB-231 breast cancer cell line expressing luciferase, and established a new tumor cell line 5a-D-Luc-ZsGreen expressing both luciferase and ZsGreen1. (spandidos-publications.com)
  • Enzymatic bioluminescence using luciferase derived from the firefly ( Photinus pyralis ) is a widely used method for image-based cell tracking in vivo . (bio-protocol.org)
  • Benefits of luciferase bioluminescence in cell tracking include penetration of tissue for non-invasive monitoring and the re-usability of the enzymatic marker. (bio-protocol.org)
  • Firefly luciferase (FLuc) is frequently used as a reporter in high-throughput screening assays owing to the exceptional sensitivity, dynamic range, and rapid measurement that bioluminescence affords. (ku.edu)
  • The aim of this research was to analyze the effects and the modes of action of elemental sulfur (So) in bioluminescence and respiration of Vibrio fischeri cells and the enzymes crude luciferase, pure catalase, and alcohol dehydrogenase (ADH). (lu.se)
  • Bioluminescence relies on the oxidation of a luciferin substrate catalysed by a luciferase enzyme. (figshare.com)
  • The name was first used by Raphaël Dubois who invented the words luciferin and luciferase, for the substrate and enzyme, respectively. (wikipedia.org)
  • However, unlike fluorescent proteins, luciferases do not require an external light source, but do require addition of luciferin, the consumable substrate. (wikipedia.org)
  • The substrate is then available for oxidation by the luciferase, where it is degraded to coelenteramide with a resultant release of energy. (wikipedia.org)
  • There is a large pocket in the β-barrel of the dinoflagellate luciferase at pH 8 to accommodate the tetrapyrrole substrate but there is no opening to allow the substrate to enter. (wikipedia.org)
  • During a reaction, luciferase has a conformational change and goes into a "closed" form with the two domains coming together to enclose the substrate. (wikipedia.org)
  • CLuc uses a specific luciferin, (also known as Vargulin) which is not a substrate for Gaussia , firefly or Renilla luciferases. (neb.com)
  • The process requires the introduction of the substrate luciferin into the cells, because the reaction of the substrate with luciferase in the presence of oxygen and ATP results in light. (photonics.com)
  • This halts or quenches the first reaction, and provides the required substrate for the Renilla luciferase reaction. (news-medical.net)
  • Luciferase production, and therefore gene activity, is monitored by adding a substrate that is converted by luciferase into a detectable luminescent signal. (genomeweb.com)
  • However, Metridia luciferase is constantly secreted by the cell into the surrounding media, meaning researchers can add the appropriate substrate at any point and detect the luciferase-catalyzed luminescence without harming the cell. (genomeweb.com)
  • The kit can be used to detect the activity of any Cypridina luciferases or derivatives that use Vargulin as a substrate. (fishersci.ca)
  • This reporter-and-control combination enables simultaneous monitoring of experimental reporter and control luciferase activities in a single-read assay without the need for two-step addition of substrate reagents or quenching. (fishersci.com)
  • In general, a bioluminescent reaction requires a substrate (generically called the luciferin), an enzyme (luciferase), and the presence of molecular oxygen with or without cofactors ( 3 , 4 ). (sciencemag.org)
  • The One-Step Luciferase Assay kit combines proprietary reagent buffer and reagent substrate components to form a working solution that contains all the necessary components for simple cell lysis and luciferase quantitation. (onenucleus.com)
  • QUANTI-Luc™ contains the coelenterazine substrate for the luciferase reaction, which produces a light signal that is quantified using a luminometer and expressed as relative light units (RLU). (invivogen.com)
  • The bacterial luciferase gene cassette ( lux ) is unique among bioluminescent bioreporter systems due to its ability to synthesize and/or scavenge all of the substrate compounds required for its production of light. (mdpi.com)
  • The mechanism of light emission is broadly conserved: an enzymatic oxidation reaction by a luciferase enzyme turns a luciferin substrate into a high energy intermediate, which decays to produce light ( Shimomura, 2006 ). (elifesciences.org)
  • Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. (biocatalogue.org)
  • The indicator is composed of an engineered firefly luciferase, of which the N- and C-terminal ends are linked with a substrate sequence of caspase-3 (Asp-Glu-Val-Asp). (springer.com)
  • When activated caspase-3 digests the substrate sequence, the cyclized luciferase recovers its activity. (springer.com)
  • We explore the strategies of simultaneous imaging of both Renilla luciferase enzyme (RL) and synthetic Renilla luciferase enzyme (hRL):coelenterazine (substrate for RL/hRL) in the same living mouse. (spiedigitallibrary.org)
  • In addition to the Firefly luciferase substrate D-Luciferin, we also provide a wide range of coelenterazine substrates for the Renilla luciferase catalysed reaction (see order information). (promokine.info)
  • Natural substrate of firefly luciferase. (axonmedchem.com)
  • We here report that the PHI JNJ-42041935 (JNJ-1935) is structurally similar to the firefly luciferase substrate D-luciferin. (uzh.ch)
  • Several enzymes are commonly used as reporter proteins, among them are chloramphenicol acetyl transferase (CAT), -galactosidase, human growth hormone (hGH) and luciferase. (abcam.com)
  • Luciferase has become one of the widely used reporter enzymes. (abcam.com)
  • Luciferase enzymes isolated from different animal species have inherent variability in light emission, allowing two or more luciferase enzymes to be used in combination for multiplex analyses, including in vivo imaging, cell viability and single and dual-spectral luciferase reporter assays. (thermofisher.com)
  • Luciferases are enzymes that act to bring about the oxidation of luciferins . (biology-online.org)
  • Luciferase enzymes present in a wide range of organisms have gained popularity in reporter systems, because of their inbuilt sensitivity and effortless measurement. (news-medical.net)
  • Luciferase is the general term given to a class of oxidative enzymes that catalyze reactions that give off bioluminescent light. (onenucleus.com)
  • The DLR assay is a two-step reaction that uses two luciferase enzymes, Firefly and Renilla (Figure 1). (bmglabtech.com)
  • Our Firefly & Renilla Luciferase Single Tube Assay now features 1X Passive Lysis Buffer 2.0, which can be used with recombinant luciferase enzymes without the addition of stabilizer. (biotium.com)
  • Conti E, Franks NP, Brick P (1996) Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes. (springer.com)
  • This study provides insights into a previously unknown JNJ-1935 off-target effect as well as into the chemical requirements for firefly luciferase and PHD inhibitors and may inform the development of novel compounds targeting these enzymes. (uzh.ch)
  • Luciferins and luciferases are generic terms used to describe a large variety of substrates and enzymes. (figshare.com)
  • Whereas luciferins can be shared by phylogenetically distant organisms which feed on organisms producing them, luciferases have been thought to be lineage-specific enzymes. (figshare.com)
  • Our findings strongly indicate that those enzymes, belonging to the haloalkane dehalogenase family, might then have been convergently co-opted into luciferases in cnidarians and echinoderms. (figshare.com)
  • In this organism, the luciferase (Renilla-luciferin 2-monooxygenase) is closely associated with a luciferin-binding protein as well as a green fluorescent protein (GFP). (wikipedia.org)
  • The N-terminal domain is conserved between dinoflagellate luciferase and luciferin binding proteins (LBPs). (wikipedia.org)
  • The chemical reaction catalyzed by firefly luciferase takes place in two steps: luciferin + ATP → luciferyl adenylate + PPi luciferyl adenylate + O2 → oxyluciferin + AMP + light Light is produced because the reaction forms oxyluciferin in an electronically excited state. (wikipedia.org)
  • Firefly luciferase generates light from luciferin in a multistep process. (wikipedia.org)
  • This shows that luciferase can differentiate between the isomers of the luciferin structure. (wikipedia.org)
  • Luciferases from click beetles, fireflies, and railway worms catalyze the ATP-dependent decarboxylation of luciferin (Figure 1). (encyclopedia.com)
  • Luciferase catalyses the oxygenation of luciferin using ATP and oxygen to produce oxyluciferin, a highly unstable, singlet-excited compound that emits light upon relaxation to its ground state. (thermofisher.com)
  • The firefly beetle ( Photinus pyralis ) contains Firefly luciferase - a 61 kDa monomeric enzyme that is capable of catalyzing the oxidation of luciferin while discharging light at about 560 nm. (news-medical.net)
  • Here, we report that Parapriacanthus ransonneti , a bioluminescent fish, obtains not only its luciferin but also its luciferase enzyme from bioluminescent ostracod prey. (sciencemag.org)
  • Luciferases from FIREFLIES, usually Photinus, that oxidizes FIREFLY LUCIFERIN to cause emission of PHOTONS. (umassmed.edu)
  • For example, an enzyme known as luciferase in fireflies produces light by acting on a molecule called luciferin. (elifesciences.org)
  • We also demonstrate that hRL:coelenterazine can yield a higher signal when compared to Firefly luciferase enzyme (FL): D-Luciferin, both in cell culture studies and when imaged from cells at the surface and from lungs of living mice. (spiedigitallibrary.org)
  • The synthesized luciferase catalyzes the luminescent reaction of luciferin with O2 and ATP. (uncg.edu)
  • The amino acid sequences on the surface of the two domains facing each other are conserved in bacterial and firefly luciferase, thereby strongly suggesting that the active site is located in the cleft between the domains. (wikipedia.org)
  • Firefly luciferase is much brighter than the bacterial luciferase sometimes used for chemiluminescence studies. (photonics.com)
  • Researchers have developed a way to use firefly luciferase - which is much brighter than bacterial luciferase - as a chemiluminescent reporter in bacteria. (photonics.com)
  • The following antibody was used in this experiment: Bacterial luciferase Polyclonal Antibody, Biotin from Thermo Fisher Scientific, catalog # PA1-26227, RRID AB_794784. (thermofisher.com)
  • PA1-26227 detects bacterial Luciferase from Photobacterium fischerii samples. (thermofisher.com)
  • Bacterial luciferase (LuxAB) was exploited in studying the wax ester metabolism in Acinetobacter baylyi ADP1. (ebscohost.com)
  • In this transgenic mouse, the firefly luciferase gene was introduced into the translation start site of the mouse Bdnf gene using a bacterial artificial chromosome (BAC). (nature.com)
  • Close D, Xu T, Smartt A, Rogers A, Crossley R, Price S, Ripp S, Sayler G. The Evolution of the Bacterial Luciferase Gene Cassette ( lux ) as a Real-Time Bioreporter. (mdpi.com)
  • Bacterial DnaJ protein could replace mammalian dj2 in the refolding of luciferase. (rupress.org)
  • Crystal structure of the bacterial luciferase/flavin complex provides insight into the function of the beta subunit. (sigmaaldrich.com)
  • Bacterial luciferase from Vibrio harveyi is a heterodimer composed of a catalytic alpha subunit and a homologous but noncatalytic beta subunit. (sigmaaldrich.com)
  • Specific transfer and expression of bacterial luciferase genes via bacteriophages provides an efficient way to detect and assay viable host cells. (asm.org)
  • Construction Of A Novel Functional Bacterial Luciferase By Gene Fusion And Its Use As A Gene Marker In Low Light Video Image Analysis. (spie.org)
  • We have used the gene encoding the monomeric bacterial luciferase as a gene marker in prokaryotic and eukaryotic organisms. (spie.org)
  • This study examines the structure of the active center of bacterial luciferase by exploiting the existence of two well-documented structural features of the (alpha) subunit of the enzyme, the essential cysteine and the protease-labile region, which are thought to reside there. (illinois.edu)
  • This Pierce Luciferase Glow Assay Kit contains reagents for measuring Cypridina luciferase activity in mammalian cell culture media and lysates. (fishersci.ca)
  • The Thermo Scientific Pierce Cypridina-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates. (fishersci.com)
  • The Pierce Cypridina -Firefly Luciferase Dual Assay Kit is a highly sensitive assay that allows for the simultaneous detection of Cypridina and Red Firefly luciferase activity. (fishersci.com)
  • This product is discontinued, and replaced by Renilla Luciferase Assay Kit 2.0 (catalog no. 30082). (biotium.com)
  • In this application note, we demonstrate the measurement of luciferase expression in CHO-K1 cells using the SpectraMax ® Glo Steady-Luc™ Reporter Assay Kit, which affords longlasting luminescence signals. (moleculardevices.com)
  • The SpectraMax® DuoLuc™ Reporter Assay Kit enables highly sensitive quantitation of both firefly and Renilla luciferases in mammalian cells. (moleculardevices.com)
  • Here, our improved Luciferase Reporter Assay Kit III (Firefly & Renilla Single-tube Assay) provides optimal convenience and highest sensitivity. (promokine.info)
  • Dual Luciferase Reporter Gene Assay Kit is used for sequential measuring the activity of two different luciferases, firefly (FFL) and Renilla (RL), in a single sample. (creativebiomart.net)
  • In addition, its improved luminescence module is capable of measuring multicolor assays with up to five varied luciferases, and also provides spectral scanning of luminescent substances. (news-medical.net)
  • Automated addition of firefly working solution by injection initiates the firefly luciferase luminescence reaction, and subsequent addition of Renilla working solution via a second injection quenches the firefly luminescence and adds Aquaphile™ coelenterazine to initiate the Renilla reaction. (moleculardevices.com)
  • Lemasters JJ, Hackenbrock CR (1977) Kinetics of product inhibition during firefly luciferase luminescence. (springer.com)
  • The firefly luciferase luminescence is measured first by addition of the FFL Reagent. (creativebiomart.net)
  • The RL Reagent simultaneously quenches the firefly luciferase luminescence and initiates the Renilla luciferase reaction. (creativebiomart.net)
  • Wells containing a cell lysate sample were injected with 100 μl of the firefly luciferase reagent (LAR II). (news-medical.net)
  • Assay kits based on firefly luciferase are sold by a slew of molecular biology reagent vendors. (genomeweb.com)
  • QUANTI-Luc™ is a lyophilized assay reagent containing all the components required to quantitatively measure the activity of Lucia luciferase and other coelenterazine-utilizing luciferases. (invivogen.com)
  • The Luciferase Quamtum Dpt reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (iowaodes.com)
  • The present invention relates to a method for producing Cypridina luciferase labeled with hydrophilic biotin, characterized in that a biotin reagent containing a polyalkylene glycol structure as a spacer is reacted with Cypridina luciferase, and biotin-labeled Cypridina luciferase wherein a sugar chain in Cypridina luciferase has been biotinylated. (freepatentsonline.com)
  • 1. A method for producing Cypridina luciferase labeled with biotin, characterized in that a biotin reagent containing a polyalkylene glycol structure as a spacer is reacted with Cypridina luciferase. (freepatentsonline.com)
  • 4. A method for producing Cypridina luciferase wherein a sugar chain moiety has been biotinylated, comprising a step of introducing an aldehyde group in the sugar chain moiety by treating Cypridina luciferase having a sugar chain with a periodate salt and a step of subsequently reacting the aldehyde group in Cypridina luciferase having the sugar chain with a biotinylation reagent which selectively reacts with the aldehyde group. (freepatentsonline.com)
  • Lucia luciferase activity can be detected and quantified directly in the culture medium of transfected cells using InvivoGen's QUANTI-Luc™ detection reagent. (invivogen.com)
  • 1 min, 0.2 ml luciferase reagent was added and emission of light was measured in a luminometer. (eurekamag.com)
  • Light emission in some bioluminescent bacteria utilizes 'antenna' such as lumazine protein to accept the energy from the primary excited state on the luciferase, resulting in an excited lulnazine chromophore which emits light that is of a shorter wavelength (more blue), while in others use a yellow fluorescent protein (YFP) with FMN as the chromophore and emits light that is red-shifted relative to that from luciferase. (wikipedia.org)
  • The gene encodes a bioluminescent Green Renilla luciferase (36kDa). (fishersci.ca)
  • When used with Thermo Scientific Cypridina Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of luciferase activity. (fishersci.ca)
  • The enzyme purified from the fish's light organs was identical to the luciferase of Cypridina noctiluca , a bioluminescent ostracod that they feed upon. (sciencemag.org)
  • For bioluminescent imaging, we offer reporter genes luciferase, firefly luciferase (FLuc) and Renilla luciferase mRNAs. (trilinkbiotech.com)
  • Methods of introducing bioluminescent genes, such as firefly luciferase, by viral transduction has allowed for the production of tumor cell lines that can be followed in vivo longitudinally over long periods of time. (bio-protocol.org)
  • The Metridia longa secreted luciferase gene encodes a 24 kDa protein containing an N-terminal secretory signal peptide of 17 amino acid residues. (wikipedia.org)
  • Dinoflagellate luciferase is a multi-domain eukaryote protein, consisting of an N-terminal domain, and three catalytic domains, each of which preceded by a helical bundle domain. (wikipedia.org)
  • The protein structure of firefly luciferase consists of two compact domains: the N-terminal domain and the C-terminal domain. (wikipedia.org)
  • Gaussia Luciferase (GLuc) is a recently discovered, naturally secreted protein by the copepod, Gaussia princeps (1). (neb.com)
  • In addition, the light produced by the luciferase can be absorb ed and reemitted at a different wavelength by another protein. (everything2.com)
  • Fusion protein corresponding to Firefly Firefly Luciferase. (abcam.com)
  • Hybridoma produced by the fusion of splenocytes from mice immunized with luciferase protein isolated from Photinus pyralis. (abcam.com)
  • The use of an antibody to detect luciferase can provide an alternative detection assay which directly detects luciferase protein levels, and thus has the advantage that it does not require luciferase activity and is not dependent on rapid kinetics. (abcam.com)
  • Reacts with Luciferase (Firefly) Protein. (abcam.com)
  • The Green Renilla luciferase is a 36kDa protein produced by a derivative of the wild type Renilla luciferase gene from the sea pansy, Renilla reniformis. (abcam.com)
  • The CRE Secreted Luciferase Reporter System is designed to monitor the activation of cAMP binding protein (CREB) and cAMP-mediated signal transduction pathways. (clontech.com)
  • Hi everyone, I'm doing a Renilla luciferase assay to measure the activity of protein promoter "A", and I'm transfecting with another plasmid, which is the protein "A" fused to GFP, so my question is, if I have some measures problems when measuring luciferase activity with luminometer, I mean GFP could interfere with the measurement of luciferase? (protocol-online.org)
  • The light output generated by the luciferase reaction can be correlated to the amount of luciferase protein produced, which in turn is proportional to the activity of the promoter driving the luciferase expression. (fishersci.ca)
  • The signal produced correlates to the amount of luciferase protein expressed, indicating promoter activity in the reporter assay. (invivogen.com)
  • Firefly luciferase, a 61 kD monomeric protein, is especially attractive to many researchers because of its high sensitivity, wide linear detection range and extremely low background due to the absence of endogenous luminescent activity in mammalian cells. (moleculardevices.com)
  • Firefly luciferase is a 62-kDa protein that is active as a monomer and does not require subsequent processing for its activity. (moleculardevices.com)
  • Berovic N, Pratontep S, Bryant A, Montouris A, Green RG (2002) The kinetics of radiation damage to the protein luciferase and recovery of the enzyme activity after irradiation. (springer.com)
  • Then co-transfected with CXCL12 3'UTR luciferase reporter plasmid and miR-5100 mimics or negative control into HEK293T cells, analysis the luciferase activity 48 hours after transfection, the transfection efficiency was confirmed by transfection of green fluorescent protein GFP. (vde-verlag.de)
  • We have previously shown that the peroxisomal targeting signal in firefly luciferase consists of the COOH-terminal three amino acids of the protein, serine-lysine-leucine (Gould, S.J., G.A. Keller, N. Hosken, J. Wilkinson, and S. Subramani, 1989. (rupress.org)
  • The concentration of the bulk of luciferase protein did not reflect these patterns but maintained a relatively constant level, implying that a post-translational mechanism(s) leads to the high-amplitude regulation of luciferase activity. (plantcell.org)
  • The noninvasive character of this method using light emitted from luciferase-expressing bioreporter cells is applicable to living animals, and has been used to study a wide range of biomolecular functions such as gene function, drug discovery and development, cellular trafficking, protein-protein interactions, and especially tumorigenesis, cancer treatment, and disease progression. (axonmedchem.com)
  • Small volumes of Luciferase recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. (mybiosource.com)
  • Our Ready-To-Glow MetLuc Monoclonal Antibody makes it easy to detect Metridia luciferase expressed by mammalian cells, as well as recombinant Metridia luciferase, in amounts down to 20 ng using Western blot analysis. (clontech.com)
  • twinlite ™ is a dispense-and-read ultra-high sensitivity dual luciferase reporter gene assay system for the quantitation of Firefly and Renilla luciferase expression in mammalian cells. (perkinelmer.com)
  • Firefly luciferase gene: structure and expression in mammalian cells. (asm.org)
  • It is a very sensitive reporter due to the lack of any endogenous luciferase activity in mammalian cells or tissue. (moleculardevices.com)
  • In the current study, we explore the expression of a novel synthetic Renilla luciferase reporter gene (hRluc) in living mice, which has previously been reported to be a more sensitive reporter than native Rluc in mammalian cells. (spiedigitallibrary.org)
  • The following antibody was used in this experiment: Firefly luciferase Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-32208, RRID AB_2549681. (thermofisher.com)
  • Does anyone have a plasmid that can be used to express firefly luciferase in S. cerevisiae? (bio.net)
  • Single mutants (C62S, C62V, C86S, C146S, C164S), double mutants (C62/146S, C62/164S, C86/146S, C146/164S), and triple mutant C62/146/164S of the Luciola mingrelica firefly luciferase carrying C-terminal His6-tag were obtained on the basis of plasmid pETL7 by site-directed mutagenesis. (springer.com)
  • The Ready-To-Glow Secreted Luciferase Reporter System includes a promoterless plasmid and is designed to insert your promoter of interest upstream of the sequence-optimized Metridia secreted luciferase reporter gene. (clontech.com)
  • I was thinking of transfecting the luciferase plasmid with a selectable vector (both plasmids being relatively the same size ~5.5kb). (protocol-online.org)
  • The experiments were performed with a 1:1 ratio of luciferase to expression plasmid. (scientistsolutions.com)
  • The Thermo Scientific™ pMCS-Renilla Luc Vector is a multiple cloning site plasmid to designed to accept a promoter sequence for study of gene regulation using the intracellular Renilla luciferase reporter. (fishersci.ca)
  • In this study, human CXCL12 3'UTR luciferase reporter plasmid was successfully constructed. (vde-verlag.de)
  • The result showed that transfection of miR-5100 mimics can decrease the luciferase activity of CXCL12 3'UTR luciferase reporter plasmid compared with the negative control group. (vde-verlag.de)
  • pNiFty3-T-Lucia plasmid is composed of three key elements: the mouse interferon beta minimal promoter, five NFAT transcription factor binding sites and a secreted luciferase (Lucia) reporter gene. (invivogen.com)
  • Site-specific introduction of the luciferase gene into the phage genome was achieved by homologous recombination in infected cells between a plasmid carrying A511 DNA flanking luxAB and phage DNA. (asm.org)
  • PromoKine's ready-to-use Luciferase Reporter Assay Kits provide complete systems for rapid, convenient, and quantitative detection of Firefly and/or Renilla luciferase luciferase activity in cultured eukaryotic cells, e.g., cells transfected with a plasmid carrying the Firefly and/or Renilla luciferase reporter genes. (promokine.info)
  • The catalyzed reaction is: coelenterazine + O2 → coelenteramide + CO2 + photon of light Newer luciferases have recently been identified that, unlike other luciferases, are naturally secreted molecules. (wikipedia.org)
  • In both pathways, luciferase initially catalyzes an adenylation reaction with MgATP. (wikipedia.org)
  • Luciferase is a generic name for an enzyme that catalyzes a light-emitting reaction. (thermofisher.com)
  • Biological researchers can take advantage of this reaction and use it as a readout for processes where a promoter region from a gene of interest is placed immediately upstream of the coding sequence for luciferase. (onenucleus.com)
  • When assayed under these conditions, light emitted from the reaction is directly proportional to the number of luciferase enzyme molecules. (promokine.info)
  • I am about to repeat a luciferase reporter experiment and need some advice concerning transfection. (scientistsolutions.com)
  • Note that the unique RenSP luciferase technology utilized in the LightSwitch Assay System eliminates the need for co-transfection of a normalizing control in most cases. (activemotif.com)
  • This dual reporting of each sample allows a quantitative result based on the normalization of the Renilla luciferase (transfection control). (bmglabtech.com)
  • Luciferase from the sea pansy Renilla reniformis is often used in multiplexed luciferase assays as a second reporter for normalizing transfection efficiency and for studying gene regulation and function. (moleculardevices.com)
  • Knock down efficiency after transfection of a constitutively luciferase-expressing cell line SKOV-3/Luc and after self-imposed transfection with pDNA, encoding luciferase, was compared and quantified by chemoluminescence assay. (uni-marburg.de)
  • Renilla luciferase is often used in conjunction with Firefly luciferase as a normalizing transfection control or for multiplex transcriptional reporter assays. (promokine.info)
  • Finally, an XhoI/XbaI fragment containing the firefly luciferase gene from the pGL3-luciferase vector (Promega) was subcloned into the XhoI/XbaI sites of 10XSTAT92E-hsp70 to generate 10XSTAT92E-luciferase. (addgene.org)
  • Drive Green Renilla luciferase expression as a reporter for gene regulation studies using vectors with a multiple cloning site (MCS) or control promoter (CMV or TK). (fishersci.ca)
  • The pCMV and pTK vectors have the luciferase gene under the CMV (Cytomegalovirus) promoter and Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, respectively. (fishersci.ca)
  • The empty 3´UTR vector contains the RenSP luciferase reporter gene and its constitutive promoter. (activemotif.com)
  • Because the constitutive promoter driving the RenSP luciferase gene is the same for all vectors, it is expected that all of the vectors will give comparable signals, as this experiment does not include miRNAs to interact with the various 3´UTR regions and change the amount of luciferase produced. (activemotif.com)
  • Assaying the expression of luciferase provides a rapid and inexpensive method for monitoring promoter activity. (asm.org)
  • We fused this promoter fragment to the firefly luciferase (Luc) gene to create a real-time reporter for regulated gene expression in intact plants. (plantcell.org)
  • The HPV16 LCRs were cloned into the pALuc vector in front of the luciferase gene in order to determine whether the above-mentioned sequence alterations affect the P97 promoter activity. (nih.gov)
  • Transgenic mice expressing the luciferase (luc) gene under the control of the heme oxygenase-1 promoter ( Ho1) were used to measure the induction of heme oxygenase in response to known toxicants. (ovid.com)
  • To explore the transcriptional regulation of MMP12 gene, a sensitive luciferase reporter HEK293 cell line for endogenous MMP12 promoter was generated by CRISPR/Cas9 technology. (ejbiotechnology.info)
  • In this protocol we describe a modified expression assay using transgenic plants expressing the luciferase gene under the control of phosphate transporter PHT1;1 promoter. (bio-protocol.org)
  • This assay was originally optimized for analyzing the repression of PHT1;1 promoter in response to arsenate As (V) which can be adapted to study the kinetics of transcriptional regulation of any gene in response to biotic or environmental stimuli measuring Luciferase activity in Arabidopsis thaliana . (bio-protocol.org)
  • Quantitative analysis was performed using L6-myoblasts stably expressing FGFR1, transfected with a luciferase-reporter vector containing elements of the FGF-responsive osteocalcin promoter. (garvan.org.au)
  • The DNA label contains a firefly luciferase coding sequence downstream from a T7 RNA polymerase promoter. (uncg.edu)
  • A reporter construct consisting of 13 kilobases of the human CYP3A4 promoter controlling the firefly luciferase gene was used to generate a transgenic mouse line [FVB/N-Tg( CYP3A4-luc )Xen]. (aspetjournals.org)
  • Dual-Luciferase Reporter Assay System from Promega has been designed to measure a couple of luciferase activities. (news-medical.net)
  • pGL4.13[luc2/SV40] firefly luciferase expression vector (Promega cat. (moleculardevices.com)
  • pGL4.75[hRluc/CMV] Renilla luciferase expression vector (Promega cat. (moleculardevices.com)
  • A novel complementation system with short peptide-inserted- Renilla luciferase (PI-Rluc) and split-RNA probes was constructed for noninvasive RNA detection. (ebscohost.com)
  • Therefore, when using firefly luciferase, scientists must lyse the cell to conduct the detection step. (genomeweb.com)
  • Kanno A., Umezawa Y., Ozawa T. (2009) Detection of Apoptosis Using Cyclic Luciferase in Living Mammals. (springer.com)
  • The utility of luciferase reporter mycobacteriophages (LRPs) for detection, identification, and antibiotic susceptibility testing of Mycobacterium tuberculosis was prospectively evaluated in a clinical microbiology laboratory in Mexico City, Mexico. (asm.org)
  • Serial injection of two optimized detection reagents allows the luciferases to be assayed in the same microplate well. (moleculardevices.com)
  • Ready-to-use kit providing a complete system for a rapid, convenient and quantitative detection of Firefly luciferase activity in eukaryotic cells. (promokine.info)
  • High sensitivity and wide detection range: detection of as little of 2 fg luciferase.Compatible with routine laboratory and HTS formats: assays can be performed in tubes or microplates, and measured with any luminometer. (creativebiomart.net)
  • Can be readily automated on HTS liquid handling systems.Fast and convenient: three step assay allows detection of dual luciferase levels within 20 minutes. (creativebiomart.net)
  • Activity of Gaussia (GLuc) and Cypridina (CLuc) Luciferases Expressed in CHO Cells. (neb.com)
  • Cypridina Luciferase (CLuc) is secreted from the marine ostracod, Cyridina noctiluc a. (neb.com)
  • Therefore, Cypridina is compatible with other luciferases in dual activity assays. (neb.com)
  • Simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates. (fishersci.com)
  • Cypridina luciferase acts as an experimental reporter with constitutively active red firefly as a normalization control. (fishersci.com)
  • 3. The method according to claim 1 wherein a position corresponding to a lysine residue K180 and/or K203 in the Cypridina luciferase in SEQ ID NO:1 has been biotinylated. (freepatentsonline.com)
  • or (ii) a structure wherein Cypridina luciferase having a sugar chain is linked with biotin through the sugar chain. (freepatentsonline.com)
  • 9. The Cypridina luciferase labeled with biotin according to claim 8 wherein n is 2 to 100. (freepatentsonline.com)
  • 10. A complex of the Cypridina luciferase labeled with biotin according to claim 5 with a polyvalent avidin substance. (freepatentsonline.com)
  • Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. (abcam.com)
  • The adsorption of the reagents onto the inner surface of the injector tube was prevented by pipetting 20 μl aliquots of a 1:50 dilution of recombinant firefly luciferase and Renilla luciferase into one row (12 wells) of a 96-well microplate. (news-medical.net)
  • Because all LightSwitch reporter constructs contain the optimized RenSP luciferase gene, you MUST use our LightSwitch Luciferase Assay Reagents to obtain optimal results. (activemotif.com)
  • Other luciferase assay reagents are not compatible with RenSP. (activemotif.com)
  • Human IgG antibody Laboratories manufactures the luciferase quamtum dpt reagents distributed by Genprice. (iowaodes.com)
  • Luciferase is a luminescent enzyme produced by Photinus pyralis, also referred to as the common eastern firefly or North American firefly. (thermofisher.com)
  • Monoclonal antibodies that inhibit the luminescent activity of the Gaussia luciferase (Gluc) have been prepared using this method. (eurekaselect.com)
  • Another advantage of luciferases is that most cells are not luminescent such that high signal-to-noise ratios can be achieved. (bio-protocol.org)
  • We reveal the development of a versatile and nonradioactive firefly luciferase in vitro transcribed (IVT) RNA-based assay. (hindawi.com)
  • Luciferases are widely used in biotechnology, for microscopy and as reporter genes, for many of the same applications as fluorescent proteins. (wikipedia.org)
  • Housekeeping control constructs contain the 3´UTRs for common housekeeping genes cloned downstream of the RenSP luciferase reporter gene. (activemotif.com)
  • Lucia is encoded by a synthetic gene derived by genetic engineering of copepod luciferase genes. (invivogen.com)
  • Firefly and Renilla luciferases are widely used as reporter genes for studying gene regulation and function, and for pharmaceutical screening. (promokine.info)
  • One well-studied luciferase is that of the Photinini firefly Photinus pyralis, which has an optimum pH of 7.8. (wikipedia.org)
  • The nucleotide sequence of the luciferase gene from the firefly Photinus pyralis was determined from the analysis of cDNA and genomic clones. (asm.org)
  • The first report of in vivo imaging using Gluc demonstrated sensitivity that is several orders of magnitude higher than the first generation luciferases, Firefly Luciferase (Fluc) and Renilla (4). (neb.com)
  • The sensitivity and high signal intensity of this luciferase molecule proves advantageous in many reporter studies. (wikipedia.org)
  • Extreme Sensitivity of the Gaussia Luciferase Reporter. (neb.com)
  • Together with the long-lasting expression and minimal background, the direct measurement of intracellular luciferase activity of living cells allows for the monitoring of killing kinetics and displays paramount sensitivity. (hindawi.com)
  • 2. Transfer samples, for instance 10 µl of media from cells containing the secreted luciferase to opaque 96-microwell plates suitable for your luminometer. (invivogen.com)
  • Ekspresjonsplasmid og reporterplasmid vart transfektert inn i COS-1 celler og luciferase aktivitet vart målt på eit luminometer. (uib.no)
  • One such example is the Metridia coelenterazine-dependent luciferase (MetLuc, A0A1L6CBM1) that is derived from the marine copepod Metridia longa. (wikipedia.org)
  • Squid use coelenterazine and a luciferase called aequorin . (everything2.com)
  • The smaller Renilla luciferase (31 kDa), also a monomeric enzyme from the sea pansy ( Renilla reniformis ), oxidizes coelenterazine and gives out light centered at 480 nm, as shown in Figure 1. (news-medical.net)
  • Measure luminosity in endpoint mode when using Lucia luciferase or in kinetic mode depending on the coelenterazine-luciferase used. (invivogen.com)
  • Renilla luciferase catalyzes coelenterazine oxidation by oxygen to produce light (Figure 1B). (moleculardevices.com)
  • Synthetic peptide within Renilla Luciferase aa 150-250. (abcam.com)
  • WB: Renilla Luciferase transfected HEK293T whole cell lysate. (abcam.com)
  • ICC/IF: Renilla Luciferase transfected HEK293T cells. (abcam.com)
  • Flow Cyt: Renilla Luciferase transfected HEK293T cells. (abcam.com)
  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293T (Human epithelial cells from embryonic kidney) cells transfected with Empty vector or Renilla Luciferase, labeling Renilla Luciferase with ab185926 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) ( ab150077 ) secondary antibody at 1/500 dilution (green). (abcam.com)
  • Nuclear staining on HEK293T cells transfected with Renilla Luciferase is observed. (abcam.com)
  • I am planning to do a dual luciferase assay using the firefly and renilla luciferase vectors. (protocol-online.org)
  • Based on the experimental set-up, the Renilla luciferase or firefly luciferase can be used as the control or experimental reporter. (news-medical.net)
  • Firefly and Renilla luciferase reactions. (news-medical.net)
  • With regard to the Dual-Luciferase Reporter Assay System, the working of Renilla luciferase and firefly luciferase were sequentially determined. (news-medical.net)
  • In this system, a cis-EMCV RNA replicon encoding the Renilla luciferase. (ebscohost.com)
  • A red-shifted Renilla luciferase for transient reporter-gene expression. (ebscohost.com)
  • The Renilla luciferase gene as a reference gene for normalization of gene expression in transiently transfected cells. (ebscohost.com)
  • twinlite is a dual luciferase reporter gene assay system designed to detect and quantitate both Firefly and Renilla luciferase sequentially from cultured cells. (perkinelmer.com)
  • The pGL4.13[luc2/SV40] firefly luciferase expression vector was diluted in OptiMEM medium to 1 µg/µL, and the pGL4.75[hRluc/CMV] Renilla luciferase expression vector was diluted to 100 ng/µL. (moleculardevices.com)
  • Three tubes were set up as follows and mixed gently: 400 µL of Opti-MEM medium + 2 µL (2 µg) of pGL4.13[luc2/SV40] firefly luciferase expression vector + 2 µL (2 ng) of pGL4.75[hRluc/CMV] Renilla luciferase expression vector. (moleculardevices.com)
  • S. Bhaumik, X. Z. Lewis, Sanjiv Gambhir, "Optical imaging of Renilla luciferase, synthetic Renilla luciferase, and firefly luciferase reporter gene expression in living mice," Journal of Biomedical Optics 9(3), (1 May 2004). (spiedigitallibrary.org)
  • Both, Firefly luciferase and Renilla luciferase show maximum light output only when substrates and co-factors are present in large quantities. (promokine.info)
  • Reporterkonstrukt vart danna ved å subklone responselement for hPXR og hERα inn i høvesvis eldfloge-luciferase vektor og renilla-luciferase vektor. (uib.no)
  • A Renilla luciferase gene was engineered into the replicon or into the full-length viral genome to monitor viral replication. (asm.org)
  • HeLa cells were transfected with 1 µg of pCMV-GLuc or pCMV-Renilla, and activity was measured from the cell supernatant (S) or cell lysate (L). Note that although Gaussia Luciferase is a secreted reporter, there is sufficient measurable activity inside the cell. (neb.com)
  • The total light emission is proportional to the luciferase activity of the sample. (abcam.com)
  • The reason is that luciferase and renilla constructs can have different activity, depending on the cell line you transfect them into. (protocol-online.org)
  • Equation 1 was then used to calculate the activity of the luciferases in the two measurements. (news-medical.net)
  • Where, Activity (%) is luciferase activity, Read 1 is first read and Read 2 is second read after a 10-minute incubation period. (news-medical.net)
  • In our experimental set up different luciferase reporter plasmids where cotransfected with expression plasmids encoding factors affecting the activity of the cotransfected luc reporter in subsequent measurements. (scientistsolutions.com)
  • iii) Performing a dose response experiment where the concentration of expression is increased until maximal suppression of luciferase activity is observed. (scientistsolutions.com)
  • Mieko Kato, Tomoki Chiba and Yoshiro Hanyu, "Inhibition of Gaussia luciferase Activity by a Monoclonal Antibody", Current Enzyme Inhibition (2011) 7: 229. (eurekaselect.com)
  • Luciferase Activity of Insect Fatty Acyl-CoA Synthetases with Synthetic Luciferins. (umassmed.edu)
  • Depending on the instrumentation employed to detect luciferase activity, we estimate this assay to be from 30- to 1,000-fold more sensitive than assaying chloramphenicol acetyltransferase expression. (asm.org)
  • Disease progression was easily monitored by repeated quantification of Gaussia princeps luciferase activity in peripheral blood. (harvard.edu)
  • Our results demonstrate that JNJ-1935 is a novel inhibitor of firefly luciferase enzymatic activity. (uzh.ch)
  • We have determined, using low light video imaging, that the activity of this novel enzyme is similar to wild type luciferase when synthesized at low temperatures (15-20°C), but that it is sensitive in vivo to higher temperatures. (spie.org)
  • The reporter cell line was then screened with bioactive small molecule library, and a small molecule Tanshinone I was found to significantly inhibit the transcriptional activity of MMP12 gene in HEK293-MMP12-T2A-luciferase-KI cell line by luciferase activity assay, which was further confirmed to inhibit the expression of MMP12 mRNA in wild-type HEK293 cells. (ejbiotechnology.info)
  • Luciferase activity can be also determined in Nicotiana benthamiana ( N. benthamiana ) using 1 cm discs of agro infiltrated leaves transferred to a microplate and proceed as described above. (bio-protocol.org)
  • Basal expression of the reporter was highest in liver and kidney, and moderate in the duodenum in male transgenic mice, whereas the basal luciferase activity was highest in the duodenum and lower in kidney and liver in females. (aspetjournals.org)
  • ATCC maintains luciferase-expressing reporter cell lines derived from the most commonly used cells in molecular imaging studies. (atcc.org)
  • These cells either constitutively or inducibly express a reporter gene (such as GFP, RFP, luciferase, or lacZ). (atcc.org)
  • The studies revealed that the cells seemed to continue to divide during a quiescent period and that the expressed firefly luciferase seemed to be transported to certain locations over the cells' growth period. (photonics.com)
  • Aldred, S. F., Collins, P., Trinklein, N. Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells. (jove.com)
  • For example, treatment of cells with cAMP or forskolin activates CREB or ATF to bind CRE, thus initiating transcription of the secreted Metridia luciferase reporter gene. (clontech.com)
  • Here, we generated a screening assay to mine inducers of Bdnf transcription in neuronal cells, using primary cultures of cortical cells prepared from a transgenic mouse strain, specifically, Bdnf-Luciferase transgenic ( Bdnf-Luc ) mice. (nature.com)
  • Demonstrating high efficiency, consistency, and excellent target cell viability, our optimized luciferase IVT RNA is used to transfect dividing and nondividing primary antigen presenting cells. (hindawi.com)
  • The ability to cotransfect the IVT RNA of the luciferase reporter and the antigen of interest into the antigen presenting cells and its simple read-out procedure render the assay high-throughput in nature. (hindawi.com)
  • The full-length, intronless luciferase gene was inserted into mammalian expression vectors and introduced into monkey (CV-1) cells in which enzymatically active firefly luciferase was transiently expressed. (asm.org)
  • C33A cells were transfected with the isolate constructs and luciferase activities were analyzed 48 h later. (nih.gov)
  • The luciferase transfected cells maintained their original properties with stable expression. (aacrjournals.org)
  • When utilized in orthotopic xenograft studies, these luciferase expressing cells formed a reliable and essential non-invasive imaging platform that improves substantially efficacy testing of anticancer drug candidates. (aacrjournals.org)
  • The EF1α-Luciferase Minicircle for In Vivo Imaging leverages SBI's easy-to-use Minicircle Technology to deliver luciferase to target cells. (systembio.com)
  • Release of ATP from somatic cells in milk with Triton X-100 was optimized for assay with firefly luciferase. (eurekamag.com)
  • Exploiting secreted luciferases to monitor tumor progression in vivo. (invivogen.com)
  • Here, we describe a new in vivo model of KIT D816V+ advanced systemic mastocytosis developed by transplantation of the human ROSAKIT D816V-Gluc mast cell line in NOD-SCID IL-2R g−/− mice, using Gaussia princeps luciferase as a reporter. (harvard.edu)
  • Nevertheless, the induction of Ho1-luc expression following exposure to these agents suggests that the Ho1-luc transgenic mouse may prove useful as a model for in vivo screening of compounds that induce luciferase expression as a marker of toxicity. (ovid.com)
  • A novel circadian phenotype based on firefly luciferase expression in transgenic plants. (plantcell.org)
  • This brings value that is different both from our fluorescent proteins and more traditional luciferases," he added. (genomeweb.com)
  • Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. (biocatalogue.org)
  • Antibodies directed against the peroxisomal targeting signal of firefly luciferase recognize multiple mammalian peroxisomal proteins. (rupress.org)
  • These results provide independent immunological evidence that the peroxisomal targeting signal identified in firefly luciferase is present in many peroxisomal proteins. (rupress.org)
  • Figueiredo, M.L. Sonodelivery Facilitates Sustained Luciferase Expression from an Episomal Vector in Skeletal Muscle. (mdpi.com)
  • Neto MF, Letteri R, Chan-Seng D, Emrick T, Figueiredo ML. Sonodelivery Facilitates Sustained Luciferase Expression from an Episomal Vector in Skeletal Muscle. (mdpi.com)
  • Colonies that formed after this period were isolated and expanded in normal medium and evaluated for luciferase expression and molecular integrity of the DNA vector. (aacrjournals.org)
  • A letter to the editor is presented regarding the use of Renilla reniformis luciferase (RLuc) for transient reporter-gene expression. (ebscohost.com)
  • Firefly luciferase is a single polypeptide specified by the luc gene that can be readily cloned into vectors used in gene delivery. (bio-protocol.org)
  • For the purpose of making luciferase expressing cell lines, lentiviral retroviral vectors derived from human immunodeficiency virus-1 (HIV-1) are highly effective. (bio-protocol.org)
  • Moreover, antibodies can detect the luciferase enzyme expression in situ, providing a means to study the localized signal sequences using luciferase as a reporter gene. (abcam.com)
  • Previously, we generated a novel transgenic mouse strain, termed Bdnf-Luciferase transgenic ( Bdnf-Luc ) mice, to measure and visualise changes in Bdnf expression using firefly luciferase as an imaging probe 19 . (nature.com)
  • Because of their similar activities, luciferase is able to replace fatty acyl-CoA synthetase and convert long-chain fatty acids into fatty-acyl CoA for beta oxidation. (wikipedia.org)
  • Compared to the wild type luciferase, Green Renilla is more stable in serum and has an the emission spectrum that is shifted toward the green region. (abcam.com)
  • The Dual-Luciferase ® Reporter Assay or DLR is widely used to study gene transcription and regulation. (bmglabtech.com)
  • They engineered BL21 and XLU102 E. coli strains to express firefly luciferase. (photonics.com)
  • The use of gene reporters such as luciferase permits highly sensitive and nondestructive monitoring of gene expression. (moleculardevices.com)
  • The unique qualities of the firefly luciferase system allowed us to monitor regulated gene expression in real time in individual multicellular organisms. (plantcell.org)
  • The assay working solution contains substrates for both luciferases, and the reactions occur simultaneously with flash-type kinetics. (fishersci.com)
  • All luciferase-based reporters work essentially the same way: At its most basic, the enzyme is co-expressed with a gene of interest, and luciferase is produced when that gene is activated. (genomeweb.com)
  • Firefly luciferase catalyzes the emission of yellow-green light and the emission color changes to red by the substitution of an amino acid. (nii.ac.jp)
  • Scientists at NEB have used Gaussia luciferase to develop siRNA potency and miRNA expression assays (5,6). (neb.com)
  • The majority of studied luciferases have been found in animals, including fireflies, and many marine animals such as copepods, jellyfish, and the sea pansy. (wikipedia.org)
  • The luciferases of fireflies - of which there are over 2000 species - and of the other Elateroidea (click beetles and relatives in general) are diverse enough to be useful in molecular phylogeny. (wikipedia.org)
  • The 5' end of the luciferase mRNA was determined by both S1 nuclease analysis and primer extension. (asm.org)

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