AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation Science
Luciferases, BacterialFirefliesLuciferases, FireflyLuciferasesFirefly LuciferinVibrionaceaeBeetlesLuminescenceLuciferases, RenillaLuminescent MeasurementsRenillaCopepodaAquatic OrganismsPhotobacteriumLuminescent AgentsPhotorhabdusProtein RenaturationColorEnzyme StabilityVibrioLuminescent ProteinsGenes, ReporterAmino Acid SequenceKineticsMolecular Sequence DataSequence AlignmentRecombinant ProteinsBinding SitesPrevotellaTransfectionMouthCell Line, TumorPromoter Regions, Genetic
Luciferases, Bacterial
Luciferases from BACTERIA such as PHOTOBACTERIUM; VIBRIO; and PHOTORHABDUS.
Fireflies
The family Lampyidae, which are bioluminescent BEETLES. They contain FIREFLY LUCIFERIN and LUCIFERASES. Oxidation of firefly luciferin results in luminescence.
Luciferases, Firefly
Luciferases from FIREFLIES, usually Photinus, that oxidizes FIREFLY LUCIFERIN to cause emission of PHOTONS.
Luciferases
Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.
Firefly Luciferin
A benzothaizole which is oxidized by LUCIFERASES, FIREFLY to cause emission of light (LUMINESCENCE).
Vibrionaceae
A family of gram-negative bacteria whose members predominate in the bacterial flora of PLANKTON; FISHES; and SEAWATER. Some members are important pathogens for humans and animals.
Beetles
INSECTS of the order Coleoptera, containing over 350,000 species in 150 families. They possess hard bodies and their mouthparts are adapted for chewing.
Luminescence
Emission of LIGHT when ELECTRONS return to the electronic ground state from an excited state and lose the energy as PHOTONS. It is sometimes called cool light in contrast to INCANDESCENCE. LUMINESCENT MEASUREMENTS take advantage of this type of light emitted from LUMINESCENT AGENTS.
Luciferases, Renilla
Luciferases from RENILLA that oxidizes certain LUMINESCENT AGENTS to cause emission of PHOTONS.
Luminescent Measurements
Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.
Renilla
A genus of bioluminescent marine invertebrates in the family Renillidae, order Pennatulacea, class ANTHOZOA. It contains Renilla LUCIFERASE which oxidizes coelenterazine resulting in LUMINESCENCE.
Copepoda
A huge subclass of mostly marine CRUSTACEA, containing over 14,000 species. The 10 orders comprise both planktonic and benthic organisms, and include both free-living and parasitic forms. Planktonic copepods form the principle link between PHYTOPLANKTON and the higher trophic levels of the marine food chains.
Aquatic Organisms
Organisms that live in water.
Photobacterium
A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that are common in the marine environment and on the surfaces and in the intestinal contents of marine animals. Some species are bioluminescent and are found as symbionts in specialized luminous organs of fish.
Luminescent Agents
Compound such as LUMINESCENT PROTEINS that cause or emit light (PHYSICAL LUMINESCENCE).
Photorhabdus
A genus of gram-negative bacteria existing symbiotically with nematodes of the family Heterorhabditidae (see RHABDITOIDEA). These nematodes infect a variety of soil-dwelling insects. Upon entering an insect host, the nematode releases Photorhabdus from its intestinal tract and the bacterium establishes a lethal septicemia in the insect.
Protein Renaturation
The reconstitution of a protein's activity following denaturation.
Color
The visually perceived property of objects created by absorption or reflection of specific wavelengths of light.
Enzyme Stability
The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat.
Vibrio
A genus of VIBRIONACEAE, made up of short, slightly curved, motile, gram-negative rods. Various species produce cholera and other gastrointestinal disorders as well as abortion in sheep and cattle.
Luminescent Proteins
Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.
Genes, Reporter
Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.
Amino Acid Sequence
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Kinetics
The rate dynamics in chemical or physical systems.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Sequence Alignment
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Recombinant Proteins
Proteins prepared by recombinant DNA technology.
Binding Sites
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Prevotella
A genus of gram-negative, anaerobic, nonsporeforming, nonmotile rods. Organisms of this genus had originally been classified as members of the BACTEROIDES genus but overwhelming biochemical and chemical findings in 1990 indicated the need to separate them from other Bacteroides species, and hence, this new genus was established.
Transfection
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Mouth
The oval-shaped oral cavity located at the apex of the digestive tract and consisting of two parts: the vestibule and the oral cavity proper.
Cell Line, Tumor
A cell line derived from cultured tumor cells.
Promoter Regions, Genetic
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.

In vivo chaperone activity of heat shock protein 70 and thermotolerance. (1/8235)

Heat shock protein 70 (Hsp70) is thought to play a critical role in the thermotolerance of mammalian cells, presumably due to its chaperone activity. We examined the chaperone activity and cellular heat resistance of a clonal cell line in which overexpression of Hsp70 was transiently induced by means of the tetracycline-regulated gene expression system. This single-cell-line approach circumvents problems associated with clonal variation and indirect effects resulting from constitutive overexpression of Hsp70. The in vivo chaperone function of Hsp70 was quantitatively investigated by using firefly luciferase as a reporter protein. Chaperone activity was found to strictly correlate to the level of Hsp70 expression. In addition, we observed an Hsp70 concentration dependent increase in the cellular heat resistance. In order to study the contribution of the Hsp70 chaperone activity, heat resistance of cells that expressed tetracycline-regulated Hsp70 was compared to thermotolerant cells expressing the same level of Hsp70 plus all of the other heat shock proteins. Overexpression of Hsp70 alone was sufficient to induce a similar recovery of cytoplasmic luciferase activity, as does expression of all Hsps in thermotolerant cells. However, when the luciferase reporter protein was directed to the nucleus, expression of Hsp70 alone was not sufficient to yield the level of recovery observed in thermotolerant cells. In addition, cells expressing the same level of Hsp70 found in heat-induced thermotolerant cells containing additional Hsps showed increased resistance to thermal killing but were more sensitive than thermotolerant cells. These results suggest that the inducible form of Hsp70 contributes to the stress-tolerant state by increasing the chaperone activity in the cytoplasm. However, its expression alone is apparently insufficient for protection of other subcellular compartments to yield clonal heat resistance to the level observed in thermotolerant cells.  (+info)

Transcriptional regulation and induction of apoptosis: implications for the use of monomeric p53 variants in gene therapy. (2/8235)

The p53 tumour suppressor protein is a transcriptional activator, which can induce cell cycle arrest and apoptosis. p53 Gene mutations occur in more than 50% of all human tumours. Reintroduction of wild-type p53 but also of oligomerisation-independent p53 variants into tumour cells by gene transfer methods has been considered. We have investigated the biological properties of two carboxy-terminal deletion mutants of p53, p53 delta 300 (comprising amino acids 1-300) and p53 delta 326 (amino acids 1-326), to evaluate their potential deployment in gene therapy. Transactivation was measured in transiently transfected HeLa and SKBR3 cells. Both monomeric variants showed reduced activities compared with wild-type p53. Individual promoters were differently affected. In contrast to wild-type p53, monomeric variants were not able to induce apoptosis. We also provided wild-type p53 and p53 delta 326 with tetracycline-regulated promoters and stably introduced these constructs into Saos2 and SKBR3 cells. Upon induction, wild-type p53 expressing cells, but not p53 delta 326 expressing cells underwent apoptosis. Consistently, only wild-type p53 expressing cells accumulated p21/waf1/cip1 mRNA and protein and showed increased bax, Gadd45 and mdm2 mRNA. Neither wild-type p53 nor p53 delta 326 repressed the transcription of the IGF-1R gene in these cell lines. We conclude that the transactivation potential of monomeric, carboxy-terminally truncated p53 is not sufficient to cause induction of the endogenous target genes which trigger apoptosis.  (+info)

A strategy for enhancing the transcriptional activity of weak cell type-specific promoters. (3/8235)

Cell type- and tissue-specific promoters play an important role in the development of site-selective vectors for gene therapy. A large number of highly specific promoters has been described, but their applicability is often hampered by their inefficient transcriptional activity. In this study, we describe a new strategy for enhancing the activity of weak promoters without loss of specificity. The basic principle of this strategy is to establish a positive feedback loop which is initiated by transcription from a cell type-specific promoter. This was achieved by using a cell type-specific promoter to drive the simultaneous expression of the desired effector/reporter gene product and a strong artificial transcriptional activator which stimulates transcription through appropriate binding sites in the promoter. Using a VP16-LexA chimeric transcription factor, we show that this approach leads to a 14- to > 100-fold enhancement of both the endothelial cell-specific von Willebrand factor promoter and the gastrointestinal-specific sucrase-isomaltase promoter while maintaining approximately 30- to > 100-fold cell type specificity.  (+info)

Ligand substitution of receptor targeted DNA complexes affects gene transfer into hepatoma cells. (4/8235)

We have targeted the serpin enzyme complex receptor for gene transfer in human hepatoma cell lines using peptides < 30 amino acids in length which contain the five amino acid recognition sequence for this receptor, coupled to poly K of average chain length 100 K, using the heterobifunctional coupling reagent sulfo-LC SPDP. The number of sulfo-LC SPDP modified poly-L-lysine residues, as well as the degree of peptide substitution was assessed by nuclear magnetic resonance spectroscopy. Conjugates were prepared in which 3.5%, 7.8% or 26% of the lysine residues contained the sulfo-LC SPDP moiety. Each of these conjugates was then coupled with ligand peptides so that one in 370, one in 1039, or one in 5882 lysines were substituted with receptor ligand. Electron microscopy and atomic force microscopy were used to assess complex structure and size. HuH7 human hepatoma cells were transfected with complexes of these conjugates with the plasmid pGL3 and luciferase expression measured 2 to 16 days after treatment. All the protein conjugates in which 26% of the K residues were modified with sulfo-LC SPDP were poor gene transfer reagents. Complexes containing less substituted poly K, averaged 17 +/- 0.5 nm in diameter and gave peak transgene expression of 3-4 x 10(6) ILU/mg which persisted (> 7 x 10(5) ILU) at 16 days. Of these, more substituted polymers condensed DNA into complexes averaging 20 +/- 0.7 nm in diameter and gave five-fold less luciferase than complexes containing less substituted conjugates. As few as eight to 11 ligands per complex are optimal for DNA delivery via the SEC receptor. The extent of substitution of receptor-mediated gene transfer complexes affects the size of the complexes, as well as the intensity and duration of transgene expression. These observations may permit tailoring of complex construction for the usage required.  (+info)

Cadmium-mediated activation of the metal response element in human neuroblastoma cells lacking functional metal response element-binding transcription factor-1. (5/8235)

Metal response element-binding transcription factor-1 (MTF-1) binds specifically to metal response elements (MREs) and transactivates metallothionein (MT) gene expression in response to zinc and cadmium. This investigation contrasts the mechanism of mouse MT gene (mMT-I) promoter activation by cadmium and zinc in IMR-32 human neuroblastoma cells to determine whether MTF-1 binding to the MRE is necessary for activation by these metals. Cadmium activated a mMT-1 promoter (-150 base pairs) luciferase reporter 20-25-fold through a MRE-dependent mechanism. In contrast, zinc had little effect on the mMT-1 luciferase reporter. IMR-32 cells lacked MRE binding activity, and treatment with zinc in vitro or in vivo did not generate a MTF-1. MRE complex, suggesting that IMR-32 cells lack functional MTF-1. Overexpression of mMTF-1 regenerated a zinc-mediated induction of the MRE without affecting cadmium activation. Because no other transition metals tested activated the MRE, this effect appeared to be cadmium-specific. These data demonstrate that in IMR-32 human neuroblastoma cells, zinc and cadmium can use independent mechanisms for activation of the mMT-I promoter and cadmium-mediated MRE activation is independent of MTF-1 and zinc.  (+info)

Gene expression and chromatin organization during mouse oocyte growth. (6/8235)

Mouse oocytes can be classified according to their chromatin organization and the presence [surrounded nucleolus (SN) oocytes] or absence [nonsurrounded nucleolus (NSN) oocytes] of a ring of Hoechst-positive chromatin around the nucleolus. Following fertilization only SN oocytes are able to develop beyond the two-cell stage. These studies indicate a correlation between SN and NSN chromatin organization and the developmental competence of the female gamete, which may depend on gene expression. In the present study, we have used the HSP70.1Luc transgene (murine HSP70.1 promoter + reporter gene firefly luciferase) to analyze gene expression in oocytes isolated from ovaries of 2-day- to 13-week-old females. Luciferase was assayed on oocytes after classification as SN or NSN type. Our data show that SN oocytes always exhibit a higher level of luciferase activity, demonstrating a higher gene expression in this category. Only after meiotic resumption, metaphase II oocytes derived from NSN or SN oocytes acquire the same level of transgene expression. We suggest that the limited availability of transcripts and corresponding proteins, excluded from the cytoplasm until GVBD in NSN oocytes, could explain why these oocytes have a lower ability to sustain embryonic development beyond the two-cell stage at which major zygotic transcription occurs. With this study we have furthered our knowledge of epigenetic regulation of gene expression in oogenesis.  (+info)

UV-A-induced decrease in nuclear factor-kappaB activity in human keratinocytes. (7/8235)

Previous reports have demonstrated an increase in nuclear factor-kappaB (NF-kappaB) activity in response to UV radiation. These studies have essentially focused on the DNA-damaging fraction of solar UV radiation (UV-B and UV-C). In contrast, the effects of UV-A radiation (320-400 nm) on NF-kappaB are not well known. In this study, we present evidence that UV-A radiation induces a marked decrease in NF-kappaB DNA-binding activity in NCTC 2544 human keratinocytes. In addition, NCTC 2544 keratinocytes pretreated with UV-A fail to respond to NF-kappaB inducers. Moreover, UV-A radiation induces a decrease in NF-kappaB-driven luciferase reporter gene expression in NCTC 2544 keratinocytes. The expression of the gene encoding IkappaBalpha (IkappaB is the NF-kappaB inhibitor), which is closely associated with NF-kappaB activity, is also reduced (3-fold) upon UV-A treatment. Our results indicate that the UV-A-induced decrease in NF-kappaB DNA-binding activity is associated with a decrease in the levels of the p50 and p65 protein subunits. This is the first evidence that an oxidative stress, such as UV-A radiation, may induce a specific decrease in NF-kappaB activity in mammalian cells, probably through degradation of NF-kappaB protein subunits. These findings suggest that UV-A could modulate the NF-kappaB-dependent gene expression.  (+info)

Modulation of human airway smooth muscle proliferation by type 3 phosphodiesterase inhibition. (8/8235)

Elevation in cell cAMP content can inhibit mitogenic signaling in cultured human airway smooth muscle (HASM) cells. We studied the effects of the type 3-selective phosphodiesterase inhibitor siguazodan, the type 4-selective phosphodiesterase inhibitor rolipram, and the nonselective inhibitor 3-isobutyl-1-methylxanthine (IBMX) on proliferation of cultured HASM cells. At concentrations selective for the type 3 phosphodiesterase isoform, siguazodan inhibited both [3H]thymidine incorporation (IC50 2 microM) and the increase in cell number (10 microM; 64% reduction) induced by platelet-derived growth factor-BB (20 ng/ml). These effects were mimicked by IBMX. At concentrations selective for type 4 phosphodiesterase inhibition, rolipram was without effect. A 20-min exposure to siguazodan and rolipram did not increase whole cell cAMP levels. However, in HASM cells transfected with a cAMP-responsive luciferase reporter (p6CRE/Luc), increases in cAMP-driven luciferase expression were seen with siguazodan (3.9-fold) and IBMX (16.5-fold). These data suggest that inhibition of the type 3 phosphodiesterase isoform present in airway smooth muscle results in inhibition of mitogenic signaling, possibly through an increase in cAMP-driven gene expression.  (+info)

PML is required for p73 transcriptional and proapoptoti | Open-iPML is required for p73 transcriptional and proapoptoti | Open-i
PML is required for p73 transcriptional and proapoptotic activity. (A) A bax promoter-driven luciferase reporter plasmid (bax-Luc) alone or in combination with
https://openi.nlm.nih.gov/detailedresult.php?img=PMC2211783_20031943f7&req=4
admin - Page 3 - islanddothers2 fun
There was a positive correlation between CEA levels and CA 15-3 levels and patient prognosis. A high specificity for B-cells was confirmed by assaying for luciferase reporter gene expression from a plasmid containing an analogous combination of immunoglobulin heavy chain regulatory ...
http://islanddothers2.fun/author/admin/page/3/
Luciferase Reporter │ Secreted Luciferase AssayLuciferase Reporter │ Secreted Luciferase Assay
Single Luciferase Reporter Assay
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
http://molecularcloning.com/index.php?prt=197
INT297671 - wiki-pain
Both the luciferase gene and a human housekeeping gene ACTB (both expressed only in human cells) were present only in the injected kidney tissue, as confirmed by the absence of the both housekeeping ACTB and luciferase gene in non-injected kidneys (Figure 3D ...
http://gnteam.cs.manchester.ac.uk/demos/wiki-pain/vhosts/wikipaints/mediawiki-1.19.1/index.php/INT297671
Thermo Scientific™ Gaussia Luciferase Reporter Assay Vectors pMCS-Gaussia Luc Vector Thermo Scientific™ Gaussia Luciferase...Thermo Scientific™ Gaussia Luciferase Reporter Assay Vectors pMCS-Gaussia Luc Vector Thermo Scientific™ Gaussia Luciferase...
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Thermo Scientific™ Pierce™ Gaussia Luciferase Flash Assay Kit 100-rxn kit Thermo Scientific™ Pierce™ Gaussia Luciferase Flash...Thermo Scientific™ Pierce™ Gaussia Luciferase Flash Assay Kit 100-rxn kit Thermo Scientific™ Pierce™ Gaussia Luciferase Flash...
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Gaussia Luciferase | NEBGaussia Luciferase | NEB
Gaussia Luciferase (GLuc) reporter gene offers bright bioluminescence, either as a standalone expression monitor or as a fusion partner with other protein.
https://www.neb.com/applications/cellular-analysis/reporter-systems/gaussia-luciferase
What is the half-life of the Gaussia luciferase (GLuc)? | NEBWhat is the half-life of the Gaussia luciferase (GLuc)? | NEB
The half-life of GLuc remains unclear. All GLuc-expressing vectors available for mammalian expression at NEB harbor the humanized codons for GLuc in which the secreted GLuc has activity similar to that of the native protein. Once GLuc is secreted into the culture medium, it is very stable. For example, ~20% of the GLuc activity was detectable after incubating a GLuc-containing sample at 99˚C for 15 minutes ...
https://www.neb.com/faqs/2012/02/11/what-is-the-half-life-of-the-gaussia-luciferase-gluc
Measurement of (anti-)oestrogenic potency in complex mixtures using a novel stably transfected luciferase reporter gene assay...
TY - GEN. T1 - Measurement of (anti-)oestrogenic potency in complex mixtures using a novel stably transfected luciferase reporter gene assay in the human T47D breast cancer cell. AU - Legler, J.. AU - van den Brink, C.. AU - Brouwer, A.. AU - van der Saag, P.. AU - Vethaak, A.D.. AU - Murk, A.J.. AU - van der Burg, B.. PY - 2000. Y1 - 2000. M3 - Conference paper. SP - 104. EP - 108. BT - Endocrine Disrupting Compounds : Wildlife and human health risks, The Hague 1998. CY - The Hague. ER - ...
https://research.wur.nl/en/publications/measurement-of-anti-oestrogenic-potency-in-complex-mixtures-using
British Library EThOS: Studies on cell division and shape in Escherichia coliBritish Library EThOS: Studies on cell division and shape in Escherichia coli
The original aim of this thesis was to utilise Vibrio harveyi luciferase as a reporter of the expression of cell division genes during the cell cycle. Several plasmids expressing luxAB genes from ftsZ promoters were constructed. To achieve maximal luciferase expression, the ribosome binding site in front of the luxA gene was improved, which led to increased expression of luciferase. The level of expression of the improved luciferase reporter from plasmids was sufficiently high to be detected in single cells, although not high enough to be used in lower copy number constructs. However, luciferase activity showed significant fluctuations that did not appear to be linked to cell cycle events. These fluctuations made the detection of any cell cycle related changes in luciferase expression impossible. Another direction of this thesis is represented by the studies on the topology of the cell shape determining RodA protein. The ampicillin resistance levels were measured in 52 fusions with the topology ...
http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.660007
Protocol Online: CachedProtocol Online: Cached
The pGL3 Luciferase Reporter Vectors provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors. The backbone of the pGL2 Luciferase Reporter Vectors was redesigned for the pGL3 Vectors for increased expression, and contains a modified coding region for firefly (Photinus pyralis) luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, the Luciferase Reporter Vectors contain numerous features aiding in the structural characterization of the putative regulatory sequences under investigation.. ...
http://www.protocol-online.org/cgi-bin/prot/view_cache.cgi?ID=1312
Plus itPlus it
p21WAF1/CIP1 plays a major role in the induction of G1 arrest following DNA damage. Although p21WAF1/CIP1 expression is regulated by the tumor suppressor p53, induction of p21WAF1/CIP1 expression through p53-independent pathways has been described in numerous cell types. In this report, we describe the mechanism by which the retinoid 6-[3-(1-adamantyl)-4-hydroxyphenyl]-2-naphthalene carboxylic acid (CD437) induces p21WAF1/CIP1 in breast carcinoma cells possessing either a wild-type (MCF-7 cells) or mutated (MDA-MB-468 cells) p53. Exposure of MDA-MB-468 cells to this retinoid results in an approximately 10-fold increase in p21WAF1/CIP1 mRNA levels, whereas less than a 2-fold increase in p21WAF1/CIP1 gene transcription was observed as indicated by transient transfection experiments utilizing a p21WAF1/CIP1 promoter firefly luciferase reporter gene construct and nuclear run-off studies. We found similar results in the MCF-7 cells (Z-M. Shao et al., Oncogene, 11: 493-504, 1995). We have now found ...
https://cancerres.aacrjournals.org/content/56/21/5055
Mutant KRAS is a druggable target for pancreatic cancer | PNASMutant KRAS is a druggable target for pancreatic cancer | PNAS
To confirm the functionality of LODER-driven siRNA in vivo, we tested the ability of a LODER containing siRNA targeting the luciferase gene, siLuc LODER, to reduce luciferase expression in normal and tumor tissues constitutively expressing the luciferase gene. We therefore implanted empty or siLuc LODERs into the livers of transgenic mice expressing the luciferase gene (MUP-Luc) in liver cells (19). In vivo imaging results showed that siLuc LODERs led to a significant decrease in luciferase levels compared with empty LODERs (Fig. S5A). Next, LODERs carrying siLuc or siGFP were implanted into CT26 cell-derived s.c. synograft tumors. Three days after implantation, measurements of luciferase activity in vivo revealed that siLuc released from the LODERs inhibited luciferase expression (Fig. S5B). This decrease in luciferase activity was not correlated to nonspecific effects on tumor growth, as tumor weights were similar within both groups (Fig. S5C). Together these results show that siRNA is ...
https://www.pnas.org/content/110/51/20723?ijkey=38c361c20f2117445509c6b3578f50ff50be7142&keytype2=tf_ipsecsha
British Pound</strong>British Pound</strong>
Luciferase Reporter Gene Assay Kit - Information The Luciferase Reporter Gene Assay is based on the quantitation of luciferase expression in mammalian, yeast or E. coil cells, using luciferin and ATP as substrates. The reaction results in light ...
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Gentaur Molecular :Panomics \ AR Luciferase Reporter Vector \ LR0007Gentaur Molecular :Panomics \ AR Luciferase Reporter Vector \ LR0007
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Measuring luciferase expression using the SpectraMax Glo Steady-Luc Reporter Assay Kit | Molecular DevicesMeasuring luciferase expression using the SpectraMax Glo Steady-Luc Reporter Assay Kit | Molecular Devices
In this application note, we measure luciferase expression in CHO-K1 cells using the SpectraMax Glo Steady-Luc Reporter Assay Kit, which affords long-lasting luminescence signals.
https://www.moleculardevices.com/en/assets/app-note/reagents/measuring-luciferase-expression-using-the-spectramax-glo-steady-luc-reporter
Luciferase Assays | LuciferaseLuciferase Assays | Luciferase
Luciferase assay products include reporter assay reagents, a comprehensive range of reporter vectors, luciferase-expressing cell lines and low-toxicity transfection reagents to ensure successful delivery of reporter vectors to the cell line of your choice.
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Get your full text copy in PDF | Medical Science MonitorGet your full text copy in PDF | Medical Science Monitor
Material and Methods: miR-150 expression was quantified by qRT-PCR in two MIBC cell lines (5637 and T24). After successful miR-150 inhibition by transfection, MTS and transwell assays were used to assess the MIBCs cisplatin sensitivity and cell invasiveness, respectively. The TargetScan database and a luciferase reporter system were used to identify whether the programmed cell death 4 protein (PDCD4) is a direct target of miR-150 in MIBC cells ...
https://www.medscimonit.com/download/index/idArt/891340
Active Motif » Search the LightSwitch 3´UTR Reporter Collection for your clone of interestActive Motif » Search the LightSwitch 3´UTR Reporter Collection for your clone of interest
The LightSwitch 3´UTR Reporter Collection from Active Motif include over 12,000 3´UTRs available as transfection-ready luciferase reporter vectors for use in reporter assays.
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IL-8 is a direct target of miR-106a. (a) Up: potential | Open-iIL-8 is a direct target of miR-106a. (a) Up: potential | Open-i
IL-8 is a direct target of miR-106a. (a) Up: potential sites in IL-8 3′UTR targeted by miR-106a. 3′UTR of IL-8 was cloned into a luciferase reporter vector.
https://openi.nlm.nih.gov/detailedresult.php?img=PMC4525765_MI2015-629862.003&req=4
Addgene: pGL3-NFAT luciferaseAddgene: pGL3-NFAT luciferase
Plasmid pGL3-NFAT luciferase from Dr. Jerry Crabtrees lab contains the insert 3x NFAT binding sequence and is published in Nature. 1992 Jun 25. 357(6380):695-7. This plasmid is available through Addgene.
http://www.addgene.org/17870/
Luciferase recombinant protein-BAF48390Luciferase recombinant protein-BAF48390
Buy Luciferase recombinant protein-BAF48390 (MBS203675) product datasheet at MyBioSource, Recombinant Proteins. Application: SDS-PAGE
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Steady-Glo® Luciferase Assay System, 10mlSteady-Glo® Luciferase Assay System, 10ml
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Japanese Yen</strong>Japanese Yen</strong>
The Dual Luciferase Reporter Assay allows for the sequential measurement of the activity of two different luciferases, firefly (FFL) and Renilla (RL), in a single sample. The firefly luciferase luminescence is measured first by addition of the FFL Reagent. Next, the RL Reagent is added to the same well. The RL Reagent simultaneously quenches the firefly luciferase luminescence and initiates the Renilla luciferase reaction. The light production of both reactions can be conveniently measured on a luminometer. This bioluminescent dual reporter gene assay is extremely sensitive and is especially suitable for quantifying dual luciferase expression in recombinant cells or in cell free transcription/translation reactions. Assays can be performed in tubes, cuvettes or multi-well plates. ...
https://www.assaygenie.com/dual-luciferase-reporter-assay-kit-ba0180/?setCurrencyId=4
Firefly Luciferase Bioluminescence as a Tool for Searching Magnetic Isotope Effects in ATP-Dependent Enzyme Reactions - статья ...Firefly Luciferase Bioluminescence as a Tool for Searching Magnetic Isotope Effects in ATP-Dependent Enzyme Reactions - статья ...
Аннотация: Сells and tissues are composed from atoms of chemical elements, some of which have two kinds of stable isotopes, magnetic and nonmagnetic ones. Not long ago, magnetic isotope effects (MIEs) have been discovered in experiments with cells enriched with magnetic or nonmagnetic isotopes of magnesium. These MIEs can stem from higher efficiency of the enzymes of bioenergetics in the cells enriched with magnetic magnesium isotope. In the studies of MIEs in biological systems, it is needed to monitor the ATP concentrations as the major energy source in cells. The most sensitive and rapid method of the ATP measurements is based on the use of the firefly luciferase-luciferin system. Since luciferase is the ATP-dependent enzyme and activated by Mg-ions, it is necessary to elucidate whether this enzyme is sensitive to magnetic field of the magnesium isotopes nuclear spin. Herein we present the results of studying the effects of different isotopes of magnesium, magnetic 25Mg and ...
https://istina.ips.ac.ru/publications/article/142960838/
The serum amyloid A3 promoter-driven luciferase reporter mice is a valuable tool to image early renal fibrosis development and...
Tubulointerstitial fibrosis is a progressive process affecting the kidneys, causing renal failure that can be life-threatening. Thus, renal fibrosis has become a serious concern in the ageing population; however, fibrotic development cannot be diagnosed early and assessed noninvasively in both patients and experimental animal models. Here, we found that serum amyloid A3 (Saa3) expression is a potent indicator of early renal fibrosis; we also established in vivo Saa3/C/EBPβ-promoter bioluminescence imaging as a sensitive and specific tool for early detection and visualization of tubulointerstitial fibrosis. Saa3 promoter activity is specifically upregulated in parallel with tumor necrosis factor α (TNF-α) and fibrotic marker collagen I in injured kidneys. C/EBPβ, upregulated in injured kidneys and expressed in tubular epithelial cells, is essential for the increased Saa3 promoter activity in response to TNF-α, suggesting that C/EBPβ plays a crucial role in renal fibrosis development. Our ...
https://ir.lib.hiroshima-u.ac.jp/en/list/HU_creator/%E3%83%A4/0f34b7dbaf99a54f520e17560c007669/item/48191
Single Or Dual Luciferase Assay - World of OdesSingle Or Dual Luciferase Assay - World of Odes
Lab Reagents Human IgG antibody Laboratories manufactures the single or dual luciferase assay reagents distributed by Genprice. The Single Or Dual Luciferase Assay reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact luciferase assay. Other Single products are available in stock. Specificity: Single Category: Or Group: Dual Luciferase. Dual Luciferase information ...
https://www.iowaodes.com/single-or-dual-luciferase-assay/
AID 95279 - Cellular activity was measured by an IL-2 luciferase reporter gene assay on a Jurkat human T cell line activated by...AID 95279 - Cellular activity was measured by an IL-2 luciferase reporter gene assay on a Jurkat human T cell line activated by...
BioAssay record AID 95279 submitted by ChEMBL: Cellular activity was measured by an IL-2 luciferase reporter gene assay on a Jurkat human T cell line activated by anti-CD28 and anti-TCR antibodies.
https://pubchem.ncbi.nlm.nih.gov/bioassay/95279
Dual luciferase assay - proper controls? - Cell Biology - BioForumDual luciferase assay - proper controls? - Cell Biology - BioForum
Dual luciferase assay - proper controls? - posted in Cell Biology: Hey everyone, I am trying to perform a dual luciferase reporter assay in a cell line with promoters established from another cell line. cDNA and protein of the gene are present in both cell lines. Problem: In the new cell line, the luciferase activity for my promoters of interest is below pGL3, whereas TKpGL3 is as highly active as in the other cell line. Renilla activity was equal in all probes. So I am looking for appr...
http://www.protocol-online.org/forums/topic/21804-dual-luciferase-assay-proper-controls/
pMV306hsp+Lux reporter plasmid - Quicktech
Bioluminescent Reporter plasmid to express bacterial luciferase (Lux) in Mycobacteria Background. Bioluminescence, the production of light by luciferase-catalysed reactions, is a versatile reporter technology with multiple applications both in vitro and in vivo. In vivo bioluminescence imaging (BLI) represents one of its most outstanding uses by allowing the non-invasive localisation of luciferase-expressing cells within an animal. Applied to the study of infectious diseases, BLI permits the detection of microorganisms from within living animals thus allowing the spatiotemporal study of infection in real-time in the same host. Moreover, using luciferase as a reporter of gene expression, it is possible to establish when and where a gene function is needed, shedding light on bacterial pathogenesis. Finally, BLI constitutes an easy and rapid method to test novel antimicrobial compounds in vivo.. Three main luciferin-luciferase systems have been utilised for BLI. The first system is represented by ...
http://quicktech.imperialinnovations.co.uk/i/Plasmids/pmv306plasmids.html
LOW MOLECULAR WEIGHT BRANCHED POLYAMINES FOR DELIVERY OF BIOLOGICALLY ACTIVE MATERIALS - Patent applicationLOW MOLECULAR WEIGHT BRANCHED POLYAMINES FOR DELIVERY OF BIOLOGICALLY ACTIVE MATERIALS - Patent application
0140] The in vitro transfection efficiency of complexes prepared with modified bPEI-2 polymer and luciferase reporter gene was investigated using HepG2 and SK-OV-3 cell lines. HepG2 cells were seeded onto 24-well plates at a density of 8×104 cells per 500 microliters per well for luciferase gene delivery. SK-OV-3 cells were seeded onto 24 well plates at a density of 8×104 cells per 500 microliters per well for luciferase gene delivery. After 24 hours, the plating media were replaced with fresh growth media, followed by the drop-wise addition of 50 microliters of complex solution containing 2.5 micrograms luciferase plasmid DNA at various N/P ratios. Following 4 hours of incubation, free complexes were removed by replacing the medium in each well. After a further 68 hours of incubation, the cell culture medium in each well was removed and the cells rinsed once with 0.5 mL of phosphate-buffered saline (PBS, pH 7.4). For luciferase expression assay, 0.2 mL of reporter lysis buffer was added to ...
http://www.patentsencyclopedia.com/app/20140073048
Most recent papers with the keyword biophotonics | Read by QxMDMost recent papers with the keyword biophotonics | Read by QxMD
The temporal effects of luciferase reaction luminescence have only been discussed in the context of light intensity (flash vs. glow). However, alterations in the color of the light emitted over the course of the luciferase reaction have not been reported. Here, we show a temporal change in the light color emitted during the reaction catalyzed by unmodified firefly luciferase when concentrations of one of the substrates, adenosine triphosphate (ATP), are gradually increased. The temporal color change from green to red occurs within the first few minutes of the luciferase reaction when an ATP-containing solution is either added or synthesized in situ with the aid of an autocatalytic reaction occurring simultaneously ...
https://www.readbyqxmd.com/keyword/87464
Data Availability StatementThe datasets used during the present study are available from the corresponding author upon...Data Availability StatementThe datasets used during the present study are available from the corresponding author upon...
Data Availability StatementThe datasets used during the present study are available from the corresponding author upon reasonable request. negative control using Lipofectamine? 3000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.). After 48 h, cells were lysed and assayed for luciferase activity using a dual luciferase reporter assay (Promega Corporation). Normalized luciferase activity was reported as luciferase activity/luciferase activity. Statistical analysis All data are presented as the mean standard deviation. SPSS 21.0 statistical software (IBM Corp., Armonk, NY, USA) was used to explore the statistical analysis. Comparisons between two groups were conducted using two-tailed Students t-test and multiple group comparisons were conducted via one-way analysis of variance with Tukeys post hoc test. P 0.05 was considered to indicate a statistically significant difference. Results miR-106b-3p is upregulated in ESCC tissues and cell lines The expression of miR-106b-3p in 50 paired ESCC ...
http://lxsustentavel.com/%EF%BB%BFdata-availability-statementthe-datasets-used-during-the-present-study-are-available-from-the-corresponding-author-upon-reasonable-request/
Luminometer, Luminometer Suppliers and Manufacturers at Alibaba.comLuminometer, Luminometer Suppliers and Manufacturers at Alibaba.com
Alibaba.com offers 155 luminometer products. About 27% of these are testing equipment, 25% are other measuring & analysing instruments, and 6% are other optics instruments. A wide variety of luminometer options are available to you,
http://www.alibaba.com/showroom/luminometer.html
Effects of APC De-Targeting and GAr Modification on the Duration of Luciferase Expression from Plasmid DNA Delivered to...Effects of APC De-Targeting and GAr Modification on the Duration of Luciferase Expression from Plasmid DNA Delivered to...
Title:Effects of APC De-Targeting and GAr Modification on the Duration of Luciferase Expression from Plasmid DNA Delivered to Skeletal Muscle. VOLUME: 15 ISSUE: 1. Author(s):Maria C. Subang, Rewas Fatah, Ying Wu, Drew Hannaman, Jason Rice, Claire F. Evans, Yuti Chernajovsky and David Gould. Affiliation:Bone & Joint Research Unit, Queen Mary University of London, William Harvey Research Institute, Charterhouse Square, London EC1M 6BQ, UK.. Keywords:Gene therapy, luciferase, microRNA, plasmid DNA, skeletal muscle, tissue-specific promoter, transgene immunogenicity.. Abstract:Immune responses to expressed foreign transgenes continue to hamper progress of gene therapy development. Translated foreign proteins with intracellular location are generally less accessible to the immune system, nevertheless they can be presented to the immune system through both MHC Class I and Class II pathways. When the foreign protein luciferase was expressed following intramuscular delivery of plasmid DNA in outbred ...
https://www.eurekaselect.com/126119/article
Supplementary Figure 1: MicroRNA represses target proteins without causing mRNA decay. | Nature Structural & Molecular BiologySupplementary Figure 1: MicroRNA represses target proteins without causing mRNA decay. | Nature Structural & Molecular Biology
a,b, Western blotting analysis of miR-20a target genes in response to increasing doses of miR-20a mimic (a) or antagomir (b). c,d, RT-qPCR analysis of miR-20a levels in HeLa cells transfected with miR-20a mimic (c) or antagomir (d). e,f, RT-qPCR analysis of DAPK3 mRNA levels in HeLa cells transfected with miR-20a mimic (e) or antagomir (f). g, Luciferase reporter constructs containing the miR-20a MRE from DAPK3 (native) in Renilla 5 UTR or mutated to restore base-pairing in the 5 end (seed) coupled with progressive mismatches (3MM+seed) in the 3 end. h,i, Results of the luciferase reporter assays (h) and quantification of luciferase mRNA (i). j,k, mRNA levels of luciferase reporters containing individual MREs from 4 indicated genes that function in both CDS and 3UTR (j) or those that function only in CDS (k), as shown in main Fig. 2e,f. l,m, Luciferase activities from the reporters containing individual MREs from 4 indicated genes that function only in CDS in response to co-transfected ...
https://www.nature.com/articles/s41594-018-0136-3/figures/7?error=cookies_not_supported&code=4c63d8fb-f259-43f4-afde-ad92257085c6
Development and validation of cell-based luciferase reporter gene assays for measuring neutralizing anti-drug antibodies...Development and validation of cell-based luciferase reporter gene assays for measuring neutralizing anti-drug antibodies...
Neutralizing anti-drug antibodies (NAbs) against therapeutic interferon beta (IFNβ) in people with multiple sclerosis (MS) are measured with cell-based bioassays. The aim of this study was to redevelop and validate two luciferase reporter-gene bioassays, LUC and iLite, using a cut-point approach to …
https://pubmed.ncbi.nlm.nih.gov/26779831/
Luciferase mRNA Transfection of Antigen Presenting Cells Permits Sensitive Nonradioactive Measurement of Cellular and Humoral...Luciferase mRNA Transfection of Antigen Presenting Cells Permits Sensitive Nonradioactive Measurement of Cellular and Humoral...
Cells transfected with 8 pmol of luc2-encoding IVT RNA were harvested at different time points to measure luminescence from 1 × 104 cells (Bright-Glo Luciferase Assay Kit for 96-well plates (Promega)). Results are the mean ± SD luminescence. Percent luminescence is relative to the highest luminescence signal obtained in each experiment. (c) Viability, reporter gene expression of iDCs and mDCs after eGFP and luc2 electroporation and phenotype after electroporation are depicted in descending order, respectively. iDCs (left panel) and mDCs (right panel) of 2 different donors were transfected with 10 μg eGFP- or luc2-encoding IVT RNA. Negative controls: cells electroporated without RNA (mock) and unelectroporated (no ep) cells. Cells were harvested at different time points. Viability and HLA-DR, CD83, and eGFP expression levels were determined by flow cytometry. Luciferase activity of 1 × 104 viable cells was measured by luminescence in triplicate ...
https://www.hindawi.com/journals/jir/2016/9540975/fig1/
Supernatants from three individual wells per experimental condition were collected in the indicated time points and assayed for...Supernatants from three individual wells per experimental condition were collected in the indicated time points and assayed for...
Supernatants from three individual wells per experimental condition were collected in the indicated time points and assayed for the IL-8 concentration using a human being IL-8 enzyme-linked immunosorbent assay (ELISA) kit according to the instructions of the manufacturer (BioLegend). et al. This content is distributed under the terms of the Creative Commons Attribution 4.0 International license. FIG?S2? Multiple strains induce TIFA-dependent signaling in epithelial cells. (A) Control, KO#1 AGS cells (DKO) were cocultured with the indicated strains (MOI = 10), and IL-8 concentration in the supernatant were measured by ELISA at 6 and 24?h. (B) NF-B luciferase activity in wild-type or strains (lysate normalized using OD600 measurements). NF-B luciferase transmission was normalized to transmission from cotransfected luciferase plasmid, and data are displayed as normalized collapse changes from mock-treated BQ-788 samples. (A and B) Data are representative of results from two self-employed ...
http://syncom.org/%EF%BB%BFsupernatants-from-three-individual-wells-per-experimental-condition-were-collected-in-the-indicated-time-points-and-assayed-for-the-il-8-concentration-using-a-human-being-il-8-enzyme-linked-i/
Licensing Nanolight TechnologyLicensing Nanolight Technology
Label License, Gaussia luciferase, material transfer license, US Patent, 6232107, 6436682, 6780974, 7045599, 7109315, 7238497, HTS, high throughput screening
https://nanolight.com/licensing_luciferases_gaussia_luciferins_fluorescent_proteins.php
CALUX Assay - - HIYOSHI Corporation
The recombinant cell line used in this assay (H1L6.1c2) was generated by stably transfecting the plasmid pGudLuc6.1 into mouse hepatoma (Hepa1c1c7) cells. The pGudLuc6.1 plasmid contains the CYP1A1 dioxin-responsive domain (inclusive of four DREs) upstream of the firefly luciferase gene ...
http://www.calux-jp.com/english/mechanism1.html
pCMV-Green Renilla Luc Sequence and MappCMV-Green Renilla Luc Sequence and Map
Control vector for constitutive high-level expression of intracellular green Renilla luciferase under control of the CMV promoter.
https://www.snapgene.com/resources/plasmid-files/?set=luciferase_vectors&plasmid=pCMV-Green_Renilla_Luc
NanoLuc® Luciferase: A Good Thing for Small PackagesNanoLuc® Luciferase: A Good Thing for Small Packages
A paper published on October 2 in the Journal of Virology describes an exciting development in the world of influenza research-the construction of a luciferase reporter virus that does not affect virulence and can be used to track development and spread of infection in mice.
http://www.promegaconnections.com/nanoluc-luciferase-a-good-thing-for-small-packages/
ATP Meets Luminometer | The Wide LineATP Meets Luminometer | The Wide Line
When I joined the lab team in May I was familiar with lab equipment and testing but I wasnt familiar with luminometers and ATP. I had heard of ATP in my
http://blog.nelsonjameson.com/atp-meets-luminometer
The LightSwitch™ 3´UTR Reporter GoClone® Collection | Active Motif LightSwitch StoreThe LightSwitch™ 3´UTR Reporter GoClone® Collection | Active Motif LightSwitch Store
Our comprehensive collection contains more than 12,000 endogenous human 3′UTR reporter GoClone® constructs that are transfection-ready with no DNA preparation required. Our reporters contain a novel luciferase gene for industry-leading sensitivity ...
http://switchgeargenomics.com/products/utr-reporter-collection?id=810234
The LightSwitch™ 3´UTR Reporter GoClone® Collection | Active Motif LightSwitch StoreThe LightSwitch™ 3´UTR Reporter GoClone® Collection | Active Motif LightSwitch Store
Our comprehensive collection contains more than 12,000 endogenous human 3′UTR reporter GoClone® constructs that are transfection-ready with no DNA preparation required. Our reporters contain a novel luciferase gene for industry-leading sensitivity ...
http://switchgeargenomics.com/products/utr-reporter-collection?id=803489
Development and Characterization of West Nile Virus Replicon Expressing Secreted Gaussia Luciferase -- Vol. 28, No 03, 2013 --...Development and Characterization of West Nile Virus Replicon Expressing Secreted Gaussia Luciferase -- Vol. 28, No 03, 2013 --...
We developed a Gaussia luciferase (Gluc) reporter replicon of West Nile virus (WNV) and used it to quantify viral translation and RNA replication. The advantage of the Gluc replicon is that Gaussia luciferase is secreted into the culture medium from cells transfected with Gluc replicon RNA, and the medium can be assayed directly for luciferase activity. Using a known Flavivirus inhibitor (NITD008), we demonstrated that the Gluc-WNV replicon could be used for antiviral screening. The Gluc-WNV-Rep will be useful for research in antiviral drug development programs, as well as for studying viral replication and pathogenesis of WNV.
http://virosin.org/Paper/2013/28/03/VS12250-013-3332-7
Frontiers | Red-Shifted Firefly Luciferase Optimized for Candida albicans In vivo Bioluminescence Imaging | MicrobiologyFrontiers | Red-Shifted Firefly Luciferase Optimized for Candida albicans In vivo Bioluminescence Imaging | Microbiology
Candida albicans is a major fungal pathogen causing life-threatening diseases in immuno-compromised patients. The efficacy of current drugs to combat C. albicans infections is limited, as these infections have a 40-60% mortality rate. There is a real need for novel therapeutic approaches, but such advances require a detailed knowledge of C. albicans and its in vivo pathogenesis. Additionally, any novel antifungal drugs against C. albicans infections will need to be tested for their in vivo efficacy over time. Fungal pathogenesis and drug-mediated resolution studies can both be evaluated using non-invasive in vivo imaging technologies. In the work presented here, we used a codon-optimized firefly luciferase reporter system for detecting C. albicans in mice. We adapted the firefly luciferase in order to improve its maximum emission intensity in the red light range (600-700 nm) as well as to improve its thermostability in mice. All non-invasive in vivo imaging of experimental animals was performed with a
https://www.frontiersin.org/articles/10.3389/fmicb.2017.01478/full
Plus itPlus it
3781 In this study, we examined transfection efficiency of naked plasmid DNA using microbubbles (Optison) with ultrasound in vitro and in vivo experiments as the feasibility of a novel nonviral vector system that transfer naked plasmid DNA into a melanoma. First, we tested the feasibility of ultrasound-mediated transfection of naked plasmid DNA in mouse melanoma B16F1 cell lines. Luciferase plasmid mixed with or without Optison was transfected into cultured mouse melanoma cells using ultrasound (1MHz; 0.4 W2) for 1 sec. Interestingly, luciferase activity was dramatically increased in cells treated with Optison, while few luciferase activity could be detected without Optison (P,0.05). We then transfected luciferase plasmid mixed with Optison by means of therapeutic ultrasound into mouse melanoma tumor. Twenty four hours after transfection, luciferase activity, transfected with Optison and ultrasound, was also higher than that of plasmid alone. Finally, we examined the feasibility of therapeutic ...
http://cancerres.aacrjournals.org/content/64/7_Supplement/872.4
Sensors | Free Full-Text | The Evolution of the Bacterial Luciferase Gene Cassette (lux) as a Real-Time Bioreporter | HTMLSensors | Free Full-Text | The Evolution of the Bacterial Luciferase Gene Cassette (lux) as a Real-Time Bioreporter | HTML
The bacterial luciferase gene cassette (lux) is unique among bioluminescent bioreporter systems due to its ability to synthesize and/or scavenge all of the substrate compounds required for its production of light. As a result, the lux system has the unique ability to autonomously produce a luminescent signal, either continuously or in response to the presence of a specific trigger, across a wide array of organismal hosts. While originally employed extensively as a bacterial bioreporter system for the detection of specific chemical signals in environmental samples, the use of lux as a bioreporter technology has continuously expanded over the last 30 years to include expression in eukaryotic cells such as Saccharomyces cerevisiae and even human cell lines as well. Under these conditions, the lux system has been developed for use as a biomedical detection tool for toxicity screening and visualization of tumors in small animal models. As the technologies for lux signal detection continue to improve, it is
http://www.mdpi.com/1424-8220/12/1/732/htm
Human Rhinovirus 16 Causes Golgi Apparatus Fragmentation without Blocking Protein Secretion | Journal of VirologyHuman Rhinovirus 16 Causes Golgi Apparatus Fragmentation without Blocking Protein Secretion | Journal of Virology
FIG 12 HRV16 infection does not block secretion of the Gaussia luciferase reporter. HeLa Ohio cells stably expressing the naturally secreted Gaussia luciferase (HeLa-Gluc) were infected with HRV16 (red squares) at an MOI of 20 for 1 h, followed by culture for the indicated periods of time up to 7 h postinfection (Time hpi). As controls, HeLa-Gluc cells were uninfected (blue circles) or uninfected but treated with BFA (green triangles). The cell culture medium was removed and replaced every 30 min, and the cells were harvested at 1-h intervals. The Gluc activity then was measured in the culture media and cell lysates. The cumulative secretion over time is calculated by adding each successive 30-min culture media sample to the previous total. Panel A shows Gluc activity in the cell lysate, and panel B shows secreted Gluc activity in the cell culture medium. Each point represents the mean relative light units (RLU) (± standard deviations) as a measure of Gluc activity from triplicate assay points ...
https://jvi.asm.org/content/88/20/11671/figures-only
Functional studies of BCL11A: characterization of the conserved BCL11A-XL splice variant and its interaction with BCL6 in...Functional studies of BCL11A: characterization of the conserved BCL11A-XL splice variant and its interaction with BCL6 in...
Chromosomal aberrations of BCL11A at 2p16.1 have been reported in a variety of B-cell malignancies and its deficiency in mice leads to a profound block in B-cell development. Alternative pre-mRNA splicing of BCL11A produces multiple isoforms sharing a common N-terminus. The most abundant isoform we have identified in human lymphoid samples is BCL11A-XL, the longest transcript produced at this locus, and here we report the conservation of this major isoform and its functional characterization. We show that BCL11A-XL is a DNA-sequence-specific transcriptional repressor that associates with itself and with other BCL11A isoforms, as well as with the BCL6 proto-oncogene. Western blot data for BCL11A-XL expression coupled with data previously published for BCL6 indicates that these genes are expressed abundantly in germinal-center-derived B cells but that expression is extinguished upon terminal differentiation to the plasma cell stage. Although BCL11A-XL/BCL6 interaction can modulate BCL6 DNA binding in
https://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-5-18
Inflence of ultrahigh frequency irradiation on Photobacterium phosphoreum luxb gene expression | The Opened International...Inflence of ultrahigh frequency irradiation on Photobacterium phosphoreum luxb gene expression | The Opened International...
Continuous increase in the number and the variety of anthropogenic sources of electromagnetic radiation causes a high interest in studying the effects ultrahigh frequency on living organisms. In the present research inflence of UHF EMR (15 W, 2.45 GHz) for 5 and 15 min on morphological and genetic peculiarities of Photobacterium phosphoreum colonies was studied. It has been revealed that UHF EMR affected colony growth parameters, induced transcriptional activity of luciferase encoding gene expression and that the effect was depended on exposure duration. The subsequent cultivation of bacteria during a two week period after treatment showed maintaining of the increased luxb mRNA level in irradiated colonies. Opposite bacterial stress responses were detected to UHF EMR and elevated temperature treatments that assumed UHF EMR comprised of not only thermal but specifi component of non-thermal nature.. The Opened International University of Human Development Ukraine ...
http://en.vmurol.com.ua/publications/131
Bioengineering in Cell and Tissue Research by Stefan Golz (auth.), Gerhard M. Artmann, Shu Chien (eds.) - Database Error Books
The attractiveness of secreted luciferases as reporters is a strong stimulus for the investigation and exploitation of new bioluminescent systems. Metridia longa is a small luminous marine copepod (Fig. 3D). The bioluminescence originates as a secretion from epidermal glands located in the head part and abdomen in response to mechanical, electrical, or chemical stimuli. Bioluminescence in Metridia longa may well serve as a defense mechanism against predators; the release of a luminous bolus from the animal is accompanied by rapid swimming that displaces the copepod away from its glowing phantom. This luciferase emits light at a peak of 480 nm with a broad emission spectrum extending to 600 nm. Gaussia luciferase has been cloned, overexpressed in bacteria, and used as a sensitive analytical reporter for hybridization assays and monitoring of cellular expression in culture and in vivo (Tannous et al. 2005). 7 Coelenterazine Dependent Luciferases 9 Fig. 3 Origins of Glow-light reporter genes used ...
http://www.justjason.com/library/bioengineering-in-cell-and-tissue-research
pCMV-GLuc 2 Control Plasmid | NEBpCMV-GLuc 2 Control Plasmid | NEB
The pCMV-GLuc 2 Control Plasmid is a mammalian expression vector that encodes the secreted luciferase from the copepod Gaussia princeps as a reporter, under the control of the constitutive CMV (cytomegalovirus) promoter. Gaussia luciferase (GLuc) is a 19 kDa protein encoded by a humanized sequence, and it contains a native signal peptide at the N-terminus that allows it to be secreted from mammalian cells into the cell culture medium. A neomycin resistance gene under the control of an SV40 promoter allows selection for stable integration of the plasmid into the mammalian cell genome using G418.
https://international.neb.com/products/n8081-pcmv-gluc-2-control-plasmid
The reporter plasmid consists of the GARP 3′ UTR cloned downstream of the Renilla luciferase gene, and a Firefly luciferase...
Since GARP appears to regulate TGF-1 secretion by T cells and TGF-one is essential for the suppressive perform of Treg cells, we sought to identify mechanisms
http://www.pgd2-receptor.com/2016/06/02/the-reporter-plasmid-consists-of-the-garp-3-utr-cloned-downstream-of-the-renilla-luciferase-gene-and-a-firefly-luciferase-gene-to-manage-for-transfection-efficiency/
Team:Bielefeld-Germany/Results/Submitted - 2010.igem.orgTeam:Bielefeld-Germany/Results/Submitted - 2010.igem.org
Bringing the firefly luciferase gene from Promegas pGL4.10[luc2] vector into a BioBrick compatible form as a sensitive reporter gene. To amplify the signal of the luciferase three different sensitivity tuners are assembled before the luciferase gene. The sensitivity tuners were created in 2007 by the iGEM team from Cambridge and amplify the read-out signal. We also assembled the firefly luciferase behind three different strong constitutive promoters for gathering additional information about this reporter gene (Cambridge, 2007). You can find this BioBrick here: BBa_K389004 You can find this BioBrick with sensitivity tuner 1 here: BBa_K389401 You can find this BioBrick with sensitivity tuner 2 here: BBa_K389402 You can find this BioBrick with sensitivity tuner 3 here: BBa_K389403 You can find this BioBrick under the control of a weak constitutive promoter here: BBa_K389302 You can find this BioBrick under the control of a medium strong constitutive promoter here: BBa_K389307 You can find this ...
http://2010.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Results/Submitted&diff=next&oldid=187046
Enzyme Substrate Bioassays | Bioscience Lab SuppliesEnzyme Substrate Bioassays | Bioscience Lab Supplies
D-Luciferin Firefly, free acid 4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid Luciferin is a common bioluminescent reporter used for in-vivo imaging of the expression of the luc marker gene . It is the substrate for the Firefly luciferase enzyme which utilizes ATP a ...
https://www.gbiosciences.com/Enzyme-Substrates?page=7
Protocols and Video Articles Authored by Andrew C. LiuProtocols and Video Articles Authored by Andrew C. Liu
Andrew C. Liu is the author of this article in the Journal of Visualized Experiments: Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
https://www.jove.com/author/Andrew+C._Liu
Background Indie luciferase reporter assays and fluorescent translocation assays have already | Selective Inhibitors of Protein...Background Indie luciferase reporter assays and fluorescent translocation assays have already | Selective Inhibitors of Protein...
Background Indie luciferase reporter assays and fluorescent translocation assays have already been successfully found in medication discovery for a number of molecular targets. would work for high throughput testing and can determine small substances that hinder FOXO signaling at different amounts. Background Forkhead package O (FOXO) proteins are growing as transcriptional integrators of pathways that regulate a number of cellular procedures, including differentiation, rate of metabolism, tension response, cell routine and apoptosis [1-3]. FOXO transcription elements have been suggested to do something as em real /em tumor suppressors because of the inhibitory results on cell routine and success [4], properties mediated by their binding as monomers to consensus DNA binding sites. Their transcriptional activity is certainly governed with a network of signaling occasions, the best known of which may be the phosphorylation of FOXO proteins at three extremely conserved serine and threonine IL6ST ...
https://onetownmanyvoices.com/background-indie-luciferase-reporter-assays-and-fluorescent-translocation-assays-have-already/
Transfection of Luciferase Reporter Plasmid Into PAC2 Zebrafish Cells - Circadian RhythmsTransfection of Luciferase Reporter Plasmid Into PAC2 Zebrafish Cells - Circadian Rhythms
We have studied the PAC-2 cell line extensively 6,7,14,15 and so have determined a set of electroporation conditions that provide optimal transfec-tion
https://www.aspeneducationgroup.com/circadian-rhythms/transfection-of-luciferase-reporter-plasmid-into-pac2-zebrafish-cells.html
JCI - Epicardial YAP/TAZ orchestrate an immunosuppressive response following myocardial infarctionJCI - Epicardial YAP/TAZ orchestrate an immunosuppressive response following myocardial infarction
Plasmids. The IFN-γ firefly-luciferase construct was generated by subcloning of specific IFN-γ-specific promoter regions (chr10:118439048-118441048, mm10) into pGL4.27 (Promega). The TEAD reporter, 8xGTIIC, murine Yap, murine Taz, and dominant-negative human TEAD1 (dnTEAD) were all described previously (76).. Cell culture and luciferase assay. HEK293T cells were maintained at 37°C with 5% CO2 in DMEM supplemented with 10% FBS, 1% penicillin, and 1% glutamate and streptomycin. All transfections were completed using FuGENE 6 (Roche). Luciferase experiments included 250 ng IFN-γ firefly-luciferase reporter constructs, 80 ng Yap or Taz, 80 ng dnTEAD, and 75 ng pGL2-Basic-Renilla luciferase (Promega). All transfections maintained an equal concentration of total DNA with transfection of pCMV-Sport6 empty vector (Invitrogen, Thermo Fisher Scientific). Cellular extracts were collected 48 hours after transfection and used in a dual-luciferase assay (Promega). Firefly luciferase activity was ...
https://wto-cn.net.www.mobile.jci.org/articles/view/88759
Jurkat-Lucia™ NFAT-CD16 cells | ADCC reporter | InvivoGenJurkat-Lucia™ NFAT-CD16 cells | ADCC reporter | InvivoGen
Jurkat-Lucia™ NFAT-CD16 cells are human reporter cells for the early nuclear translocation of NFAT upon antibody-dependent cellular cytotoxicity (ADCC) induction. A bioluminescent signal is produced by an NFAT-dependent Lucia luciferase reporter protein.
https://www.invivogen.com/jurkat-lucia-nfat-cd16-cells
November | 2018 | GFAP Signal
The cell lysates were collected for luminescence quantification using the protocol DLR-0-INJ (with 10 s integral time) of the GloMaxTM Luminometer (Promega). Idasanutlin manufacturer Ten microliter of each sample was treated with 50 μL of Luciferase Assay Reagent II to obtain the first measurement, while the second measurement was acquired upon addition of 50 μL Stop & Glo® Reagent. The ratio of the first and second luminescence readings was taken as the. level of desired plasmid activation. The Stealth siRNA (Invitrogen) designated S1, S2 or S3 were designed to target human SARM in three different domains. HEK293 cells were seeded into 24-well plates at a density of 1×105 cells/well in 0.5 mL medium, and were transfected with expression vectors and luciferase reporter genes together with siRNA for 24 h. Then the cells were harvested and divided into two halves, one for measurement of SARM mRNA level by end-point PCR and the other for luciferase assay. To examine the effect of LPS ...
https://gfap-signal.com/2018/11
Orion L Microplate Luminometer from Titertek-Berthold (Berthold Detection Systems GmbH) | SelectScienceOrion L Microplate Luminometer from Titertek-Berthold (Berthold Detection Systems GmbH) | SelectScience
Read independent reviews on Orion L Microplate Luminometer from Titertek-Berthold (Berthold Detection Systems GmbH) on SelectScience
http://www.selectscience.net/products/orion-l-microplate-luminometer/?prodID=20476&u=A69AEB43-7DA1-49C0-9086-BD607375379E&techBID=86
In other experiments, the 314 nt 39-terminal fragment of the 3UTR also increased the level of firefly luciferase transcripts |...
ences derived from the estrogen responsive Complement three (C3) or Metalloproteinase 1 (MMP1) gene. The transfection efficiency was monitored by the
http://www.sgkinhibitor.com/2017/04/19/in-other-experiments-the-314-nt-39-terminal-fragment-of-the-3utr-also-increased-the-level-of-firefly-luciferase-transcripts/
Addgene: pAAV:cTNT::LuciferaseAddgene: pAAV:cTNT::Luciferase
Plasmid pAAV:cTNT::Luciferase from Dr. William Pus lab contains the insert Firefly luciferase and is published in Circ Res. 2014 Jul 18;115(3):354-63. doi: 10.1161/CIRCRESAHA.115.303632. Epub 2014 May 15. This plasmid is available through Addgene.
https://www.addgene.org/69915/
GFP and Luciferase (firefly) Expressing Human Dermal Fibroblasts-Neonatal (GFP-Luc-HDFCs-Neo) - Regeneration biology...GFP and Luciferase (firefly) Expressing Human Dermal Fibroblasts-Neonatal (GFP-Luc-HDFCs-Neo) - Regeneration biology...
Name of Products GFP and Luciferase (firefly) Expressing Human Dermal Fibroblasts-Neonatal (GFP-Luc-HDFCs-Neo) Catalogue Number UBP-0008-NeoGFP-Luc Product
https://www.regenerationbiology.com/product/gfp-and-luciferase-firefly-expressing-human-dermal-fibroblasts-neonatal-gfp-luc-hdfcs-neo/
Identifying Targets of Human microRNAsIdentifying Targets of Human microRNAs
Description of a method for the co-transfection of individual 3UTR luciferase reporter constructs with a miRNA mimic. This method uses the LightSwitch Luciferase Assay System and the protocol is efficient, reproducible, and amenable to high-throughput analysis.
https://www.activemotif.com/human-microrna-webinar
LTA4 Hydrolase - Diversity of epothilone producersLTA4 Hydrolase - Diversity of epothilone producers
Supernatants were preincubated with Protein G Sepharose (GE Health care) for 1 h and anti-Ago2 or control IgG in 4C for 2 h accompanied by addition of 30 l of Protein G Sepharose (GE Health care) for 1 h. (C) Recognition of miR-122 appearance by Northern blot (best -panel) and qRT-PCR (bottom level). Total RNA was extracted from each cell as well as the comparative appearance Mouse monoclonal to CD31 of miR-122 was dependant on qRT-PCR through the use of U6 snRNA as an interior control. (D) miR-122 activity in miR-122-knockout Huh7 cells. pmirGLO vectors having the complementary series of miR-122 beneath the luciferase gene had been transfected into Huh7-122KO and Huh7-122KOR cells. At 48 h post-transfection, the luciferase activity was driven. The info are representative of three unbiased experiments. Mistake bars suggest the typical deviation from the mean and asterisks suggest significant distinctions (**P < 0.01) versus the outcomes for the control.(TIF) ppat.1006374.s001.tif (502K) ...
http://epothilone-b.com/category/lta4-hydrolase/
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Genetic Chickenhatching Spitzhauben For Sale FarmThe best prices on Genetic Chickenhatching Spitzhauben! Shop a wide variety of genetic chickenhatching spitzhau
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Luciferase in-vivoLuciferase in-vivo
I am interested in obtaining luminescence in E. coli in-vivo and would like information on plasmids including sequence and restriction maps. I would like to find someone who has some expertise with luciferase and perhaps has a variety of constructs available. Thanks for your help, David Rosen ...
http://www.bio.net/bionet/mm/methods/1994-July/016030.html
Luciferase Control RNA from PromegaLuciferase Control RNA from Promega
Luciferase Control RNA from Promega,in vitro Translation, Accessory Products,biological,biology supply,biology supplies,biology product
http://www.bio-medicine.org/biology-products/Luciferase-Control-RNA-from-Promega-4573-1/
Molecular Imaging with Reporter Genes: Sanjiv S. Gambhir: 9780521882330: Knjiga | Emka.siMolecular Imaging with Reporter Genes: Sanjiv S. Gambhir: 9780521882330: Knjiga | Emka.si
"Reporter genes have been used for several decades to study regulation of gene... *Hitra in zanesljiva dostava, plačilo tudi po povzetju.*
http://www.emka.si/molecular-imaging-with-reporter-genes/PR/119294
Lucía Barontini (aluciabarontini) on PinterestLucía Barontini (aluciabarontini) on Pinterest
See what Lucía Barontini (aluciabarontini) has discovered on Pinterest, the worlds biggest collection of everybodys favorite things.
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ScopesMonkey | FanFictionScopesMonkey | FanFiction
ScopesMonkey is a fanfiction author that has written 113 stories for StarTrek: Deep Space Nine, StarTrek: Other, Firefly, and Sherlock.
https://www.fanfiction.net/u/670292/
LuciferaseLuciferase
Luciferases can be produced in the lab through genetic engineering for a number of purposes. Luciferase genes can be ... In the luciferase reaction, light is emitted when luciferase acts on the appropriate luciferin substrate. Photon emission can ... Luciferase can act as an ATP sensor protein through biotinylation. Biotinylation will immobilize luciferase on the cell-surface ... in addition to the amount of luciferase. Luciferase is a heat-sensitive protein that is used in studies on protein denaturation ...
https://en.wikipedia.org/wiki/Luciferase
Firefly luciferaseFirefly luciferase
... is thought to be a homolog of long-chain fatty acyl-CoA synthetase because of its ability to synthesize ... During a reaction, luciferase has a conformational change and goes into a "closed" form with the two domains coming together to ... This shows that luciferase can differentiate between the isomers of the luciferin structure. L-luciferin is able to emit a weak ... Firefly luciferase generates light from luciferin in a multistep process. First, D-luciferin is adenylated by MgATP to form ...
https://en.wikipedia.org/wiki/Firefly_luciferase
Dinoflagellate luciferaseDinoflagellate luciferase
... (EC 1.13.12.18, Gonyaulax luciferase) is a specific luciferase, an enzyme with systematic name ... Dinoflagellate luciferase is a single protein with three luciferase domains and an N-terminal domain. The three domains have ... hnu The EC number of dinoflagellate luciferase is 1.13.12.18. This number denotes that dinoflagellate luciferase is an ... Dinoflagellate luciferase allows these organisms to emit blue light (max 475 nm) after stimulation. The light produced is ...
https://en.wikipedia.org/wiki/Dinoflagellate_luciferase
Reporter geneReporter gene
Because the reporter enzymes themselves (e.g. firefly luciferase) can be direct targets of small molecules and confound the ... Smale, S. T. (2010-05-01). "Luciferase Assay". Cold Spring Harbor Protocols. 2010 (5): pdb.prot5421. doi:10.1101/pdb.prot5421. ... Solberg, Nina; Krauss, Stefan (2013). "Luciferase assay to study the activity of a cloned promoter DNA fragment". Gene ... "A GUS/Luciferase Fusion Reporter for Plant Gene Trapping and for Assay of Promoter Activity with Luciferin-Dependent Control of ...
https://en.wikipedia.org/wiki/Reporter_gene
Bioluminescent bacteriaBioluminescent bacteria
Luciferase was first purified by McElroy and Green in 1955. It was later discovered that there were two subunits to luciferase ... utilizing luciferase and luciferin), there are different types of luciferin-luciferase systems. For bacterial bio-luminescence ... LuxAB codes for luciferase while luxCDE codes for a fatty-acid reductase complex that is responsible for synthesizing aldehydes ... All bacterial luciferases are approximately 80 KDa heterodimers containing two subunits: α and β. The α subunit is responsible ...
https://en.wikipedia.org/wiki/Bioluminescent_bacteria
Marine microbial symbiosisMarine microbial symbiosis
Luciferase enzymes produce bioluminescence. Squids can then emit the luminescence from the light organ. Because Euprymna ... After bacterial cells divide and increase in population, they begin expressing enzyme luciferase as a result of quorum sensing ...
https://en.wikipedia.org/wiki/Marine_microbial_symbiosis
E-SCREENE-SCREEN
These bioassays form a group of the so-called CALUX (Chemically Activated LUciferase eXpression) bioassays. These systems are ... firefly luciferase). That is an in vitro reporter gene assays detecting estrogen receptor (ER) activation. ... endogenously together with an ER-specific Luciferase construct. A version of the ER CALUX bioassay, consisting of the human ... U2OS osterosarcoma cell line transfected with both ERα and ER-specific Luciferase construct is currently under validation to be ...
https://en.wikipedia.org/wiki/E-SCREEN
Quorum sensingQuorum sensing
These bacteria did not synthesize luciferase-and therefore did not luminesce-in freshly inoculated culture but only after the ... However, when the population reaches the threshold in the photophore (about 1011 cells/ml), transcription of luciferase is ... Nealson KH (February 1977). "Autoinduction of bacterial luciferase. Occurrence, mechanism and significance". Archives of ...
https://en.wikipedia.org/wiki/Quorum_sensing
Alkanal monooxygenase (FMN-linked)Alkanal monooxygenase (FMN-linked)
Other names in common use include bacterial luciferase, aldehyde monooxygenase, luciferase, and Vibrio fischeri luciferase. As ... Hastings JW, Presswood RP (1978). "Bacterial luciferase: FMNH2-aldehyde oxidase". Methods Enzymol. Methods in Enzymology. 53: ...
https://en.wikipedia.org/wiki/Alkanal_monooxygenase_(FMN-linked)
John Woodland HastingsJohn Woodland Hastings
... the discovery of a flavin to be a substrate in its luciferase reaction, the determination of gene regulation of the luciferases ... that contain both the luciferase and the luciferin-binding protein. More recently, the lab found that the luciferase gene in ... controls luciferase activity by regulating turnover". J. Biol. Chem. 256 (20): 10509-10518. doi:10.1016/S0021-9258(19)68651-5. ... In other work when he was in the McElroy lab he examined the basic biochemical mechanism of firefly luciferase and demonstrated ...
https://en.wikipedia.org/wiki/John_Woodland_Hastings
MotyxiaMotyxia
The light originates by way of a photoprotein, which differs from the photogenic molecule luciferase in firefly beetles. ... and structure function relationships of insect luciferases". Cellular and Molecular Life Sciences. 59 (11): 1833-1850. doi: ...
https://en.wikipedia.org/wiki/Motyxia
Oplophorus-luciferin 2-monooxygenaseOplophorus-luciferin 2-monooxygenase
... belonging to a group of coelenterazine luciferases. Unlike other luciferases, it has a broader substrate specificity [3,4,6] ... The proteins signal the enzyme for secretion in luminescence, catalyzed by the protein 19 kDa [3,4,7]. The luciferase has many ... This mechanism causes O.gracilirostris release a luminous, bright blue luciferase cloud [2]. The small protein subunit of OpLuc ... In enzymology, an Oplophorus-luciferin 2-monooxygenase (EC 1.13.12.13), also known as Oplophorus luciferase (referred in this ...
https://en.wikipedia.org/wiki/Oplophorus-luciferin_2-monooxygenase
ArachnocampaArachnocampa
The glow is the result of a chemical reaction that involves luciferin, the substrate; luciferase, the enzyme that acts upon ...
https://en.wikipedia.org/wiki/Arachnocampa
Fluorescent glucose biosensorFluorescent glucose biosensor
16(11): p. 2350-9. Taneoka, A., et al., The construction of a glucose-sensing luciferase. Biosens Bioelectron, 2009. 25(1): p. ... Daunert makes a Ggbp-split aqueorin sensor and in 2009 Koji Sode made Ggbp-luciferase with Asp459Asn (Glc not Gal). In addition ... such as Aqueorin from symbiont in jellyfish and luciferase from symbiont in fireflies. These have been used to make glucose ...
https://en.wikipedia.org/wiki/Fluorescent_glucose_biosensor
Renilla-luciferin 2-monooxygenaseRenilla-luciferin 2-monooxygenase
... belonging to a group of coelenterazine luciferases. Of this group of enzymes, the luciferase from Renilla reniformis has been ... Renilla luciferase contains 311 amino acids, and is active as a nearly spherical single polypeptide chain monomer of 36 kDa, ... However, this enzyme appears to be unrelated from most other luciferases that act on coelenterazine, such as those from ... Loening AM, Fenn TD, Gambhir SS (December 2007). "Crystal structures of the luciferase and green fluorescent protein from ...
https://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase
PhotoproteinPhotoprotein
They add to the function of the luciferins whose usual light-producing reaction is catalyzed by the enzyme luciferase. The term ... Photoproteins do not display typical enzyme kinetics as seen in luciferases. Instead, when mixed with luciferin, they display ... rather than the prolonged glow that is seen for luciferases when luciferin is added. In this respect, it may appear that ... was meant to distinguish them from other light-producing proteins because these do not exhibit the usual luciferin-luciferase ...
https://en.wikipedia.org/wiki/Photoprotein
Aliivibrio fischeriAliivibrio fischeri
The bacterial luciferin-luciferase system is encoded by a set of genes labelled the lux operon. In A. fischeri, five such genes ... This operon has a known gene sequence of luxCDAB(F)E, where luxA and luxB code for the protein subunits of the luciferase ... Luciferase produces blue/green light through the oxidation of reduced flavin mononucleotide and a long-chain aldehyde by ... Eberhad, A (1981). "Structural identification of autoinducer of Photobacterium fischeri luciferase". PubMed. Retrieved 2020-04- ...
https://en.wikipedia.org/wiki/Aliivibrio_fischeri
N-Acyl homoserine lactoneN-Acyl homoserine lactone
"Structural identification of autoinducer of Photobacterium fischeri luciferase". Biochemistry. 20 (9): 2444-2449. doi:10.1021/ ...
https://en.wikipedia.org/wiki/N-Acyl_homoserine_lactone
Mouse models of breast cancer metastasisMouse models of breast cancer metastasis
Luciferase is injected just before taking the images. Intravital microscopy with multi photon excitation is a technique to ... The substrate luciferin, is oxidized to oxyluciferin in the presence of luciferase and emits light, which can be detected using ...
https://en.wikipedia.org/wiki/Mouse_models_of_breast_cancer_metastasis
BiosensorBiosensor
2008). "Novel Genetically Encoded Biosensors Using Firefly Luciferase". ACS Chem. Biol. 3 (6): 346-51. doi:10.1021/cb8000414. ...
https://en.wikipedia.org/wiki/Biosensor
AutoinducerAutoinducer
At low cell concentrations, V. fischeri did not express the luciferase gene. However, once the cultures had reached exponential ... luciferase) only when cultures had reached a threshold population density. ... "Structural identification of autoinducer of Photobacterium fischeri luciferase". Biochemistry. 20 (9): 2444-2449. doi:10.1021/ ... growth phase, the luciferase gene was rapidly activated. This phenomenon was termed "autoinduction" because it involved a ...
https://en.wikipedia.org/wiki/Autoinducer
Watasenia-luciferin 2-monooxygenaseWatasenia-luciferin 2-monooxygenase
This enzyme is also called Watasenia-type luciferase. Inoue S, Kakoi H, Goto, T (1976). "Squid bioluminescence. III. Isolation ...
https://en.wikipedia.org/wiki/Watasenia-luciferin_2-monooxygenase
Helmy EltoukhyHelmy Eltoukhy
Clinical Cancer Research 24(15), 3528-3538 PMID 29776953 Engineered luciferases, (2009). Biological analysis arrangement and ... US application 2009286299, Ronaghi, Mostafa; Eltoukhy, Helmy & Bazargan, Leila, "Engineered luciferases", published 2009-11-19 ...
https://en.wikipedia.org/wiki/Helmy_Eltoukhy
Howard Harold SeligerHoward Harold Seliger
There he worked with William D. McElroy, who had recently isolated the enzyme luciferase. Seliger used his measurement ... Wood, K.; Lam, Y.; Seliger, H.; McElroy, W. (1989). "Complementary DNA coding click beetle luciferases can elicit ...
https://en.wikipedia.org/wiki/Howard_Harold_Seliger
PeroxisomePeroxisome
Keller, G. A.; Gould, S.; Deluca, M.; Subramani, S. (May 1987). "Firefly luciferase is targeted to peroxisomes in mammalian ... it was described that firefly luciferase is targeted to peroxisomes in mammalian cells, allowing the discovery of the import ...
https://en.wikipedia.org/wiki/Peroxisome
TOC1 (gene)TOC1 (gene)
Millar developed an innovative forward genetic screen in which he linked a bioluminescent reporter, firefly (luciferase), to ... "Circadian clock mutants in Arabidopsis identified by luciferase imaging". Science. 267 (5201): 1161-3. Bibcode:1995Sci... ...
https://en.wikipedia.org/wiki/TOC1_(gene)
ParapriacanthusParapriacanthus
... luciferase is a kleptoprotein, obtained from their diet on bioluminescent ostracods. There are currently 11 ... Parapriacanthus fish obtain luciferase from ostracod prey". Science Advances. American Association for the Advancement of ...
https://en.wikipedia.org/wiki/Parapriacanthus
Photinus pyralisPhotinus pyralis
The light-producing enzyme is luciferase, and is found within cells of the lantern. Luciferases require oxygen, luciferin and ... "Firefly luciferase is targeted to peroxisomes in mammalian cells". Proceedings of the National Academy of Sciences. 84 (10): ...
https://en.wikipedia.org/wiki/Photinus_pyralis
SequencingSequencing
ATP powers the oxidation of luciferase through the luciferase; this reaction generates a light signal recorded as a pyrogram ...
https://en.wikipedia.org/wiki/Sequencing
Protein targetingProtein targeting
By testing a variety of mutations in the gene encoding the expressed luciferase, the consensus sequence was then determined. It ... "Firefly luciferase is targeted to peroxisomes in mammalian cells". Proceedings of the National Academy of Sciences. 84 (10): ... The initial research that led to the discovery of this consensus observed that when firefly luciferase was expressed in ...
https://en.wikipedia.org/wiki/Protein_targeting
Luciferase Assays | LuciferaseLuciferase Assays | Luciferase
... luciferase-expressing cell lines and low-toxicity transfection reagents to ensure successful delivery of reporter vectors to ... Luciferase assay products include reporter assay reagents, a comprehensive range of reporter vectors, ... Top Luciferase Assay Products for Your Lab. Nano-Glo® Dual-Luciferase Reporter Assay System. Ultra-sensitive detection of ... Luciferase Assays. Luciferase assays can be used to investigate a variety of cellular processes using bioluminescent light ...
https://www.promega.com/products/luciferase-assays/
Firefly Luciferase Assay Cell culture MilliporeFirefly Luciferase Assay Cell culture Millipore
... efficient quantitation of luciferase reporter enzyme activity from cultured cells with high sensitivity. ... The Firefly Luciferase Assay is designed for simple, ... Firefly Luciferase Assay. Flash-type luciferase assay designed ... Store Firefly Luciferase Assay at -80°C. Firefly Luciferase Assay Buffer 2.0 is stable at -20°C for three months and at -80°C ... 1) 5X Firefly Luciferase Lysis Buffer (CS224525): 2 X 15 mL. 2) Firefly Luciferase Assay Buffer 2.0 (CS224585): 100 mL. 3) D- ...
https://www.sigmaaldrich.com/SG/en/product/mm/sct154
Reconstitution of BioLux Cypridina Luciferase Substrate (E3309) | NEBReconstitution of BioLux Cypridina Luciferase Substrate (E3309) | NEB
Home Protocols Reconstitution of BioLux Cypridina Luciferase Substrate (E3309) Reconstitution of BioLux Cypridina Luciferase ... to the lyophilized BioLux Cypridina Luciferase Substrate. ...
https://www.neb.com/protocols/0001/01/01/reconstitution-of-biolux-cypridina-luciferase-substrate-e3309
Promega QuantiLum Recombinant Luciferase:Protein Analysis Reagents:Protein | Fisher ScientificPromega QuantiLum Recombinant Luciferase:Protein Analysis Reagents:Protein | Fisher Scientific
QuantiLum Recombinant Luciferase is a luciferase expressed from a cloned gene from the North American firefly (Photinus pyralis ... QuantiLum Recombinant Luciferase is a luciferase expressed from a cloned gene from the North American firefly (Photinus pyralis ... The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713; and 5,700,673. ...
https://www.fishersci.com/shop/products/promega-quantilum-recombinant-luciferase-2/p-2719379
Pierce™ Gaussia-Firefly Luciferase Dual Assay KitPierce™ Gaussia-Firefly Luciferase Dual Assay Kit
Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Gaussia and Red Firefly luciferase ... The Thermo Scientific Pierce Gaussia-Firefly Luciferase ...
https://www.thermofisher.com/order/catalog/product/16182
RCSB PDB - 2PSH: Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla ReniformisRCSB PDB - 2PSH: Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis
Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis ... Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla reniformis.. Loening, A.M., Fenn, T.D., Gambhir ... Due to its ability to emit light, the luciferase from Renilla reniformis (RLuc) is widely employed in molecular biology as a ... Due to its ability to emit light, the luciferase from Renilla reniformis (RLuc) is widely employed in molecular biology as a ...
https://www.rcsb.org/structure/2PSH
Team:Cambridge/References/ProjectBioluminescence/Luciferase - 2010.igem.orgTeam:Cambridge/References/ProjectBioluminescence/Luciferase - 2010.igem.org
Team:Cambridge/References/ProjectBioluminescence/Luciferase. From 2010.igem.org. (Difference between revisions) ...
https://2010.igem.org/wiki/index.php?title=Team:Cambridge/References/ProjectBioluminescence/Luciferase&diff=prev&oldid=30780
MAGNETIC NANOPARTICLES, LUCIFERASE & THE WEF - DR. SHERRI TENPENNYMAGNETIC NANOPARTICLES, LUCIFERASE & THE WEF - DR. SHERRI TENPENNY
FULL INTERVIEW: www.stewpeters.tv
https://www.bitchute.com/video/2Yhf9uiSNE0M/
Luciferase Substrate [Substrates for ELISA] | TCI Deutschland GmbHLuciferase Substrate [Substrates for ELISA] | TCI Deutschland GmbH
TCI uses cookies to personalize and improve your user experience. By continuing on our website, you accept the use of cookies. You can change or update your cookiesettings at any time.. ...
https://www.tcichemicals.com/DE/en/c/11531
Engineering the protein dynamics of an ancestral luciferase | Faculty of Arts MUEngineering the protein dynamics of an ancestral luciferase | Faculty of Arts MU
Structural and biochemical studies of an ancestral enzyme with dual dehalogenase and luciferase activity ... which catalyses both dehalogenase and luciferase reactions. Insertion-deletion (InDel) backbone mutagenesis of Anc(HLD-RLuc) ... network model highlights the importance of the conformational flexibility of a loop-helix fragment of Renilla luciferases for ... and fragment transplantation resulting in greater catalysis and longer glow-type bioluminescence of the ancestral luciferase. ...
https://www.phil.muni.cz/en/research/publishing-and-editorial-activities-of-the-faculty/overview-of-publishing-and-scientific-activities/1802217
pSELECT-zeo-LucSh : Firefly luciferase-Zeocin resistance fusion gene without CpG | InvivoGenpSELECT-zeo-LucSh : Firefly luciferase-Zeocin resistance fusion gene without CpG | InvivoGen
InvivoGen has engineered a fusion between the firefly luciferase (Luc) and the Sh ble genes. The firefly Luc gene is a highly ... InvivoGen has engineered a fusion between the firefly luciferase (Luc) and the Sh ble genes. The firefly Luc gene is a highly ... The Luc::Sh fusion gene exhibits a higher luciferase activity and enables a better and faster selection of Zeocin™ resistant ... LucSh: Synthetic LucSh fusion gene (LucSh-ΔCpG): InvivoGen has engineered a fusion between the firefly luciferase gene and the ...
https://www.invivogen.com/pselect-zeo-lucsh
Monitor NF-κB activation with a sensitive dual luciferase reporter assay on the SpectraMax iD5 | Molecular DevicesMonitor NF-κB activation with a sensitive dual luciferase reporter assay on the SpectraMax iD5 | Molecular Devices
Firefly luciferase is typically used as a readout for expression of a gene of interest. Luciferase from the sea pansy Renilla ... Monitor NF-κB activation with a sensitive dual luciferase reporter assay on the SpectraMax iD5 *Detect multiplexed luciferase ... and firefly luciferase levels that increased greatly with the addition of the cytokine TNF-α (Figure 2). Overall, luciferase ... Luciferase assay setup. All kit components were thawed to room temperature. Firefly Substrate was reconstituted by adding 220 ...
https://www.moleculardevices.com/en/assets/app-note/br/monitor-nf-kb-activation-with-a-sensitive-dual-luciferase-reporter-assay-on-spectramax-id5
Luciferase | Regional Anesthesia & Pain MedicineLuciferase | Regional Anesthesia & Pain Medicine
Regional Anesthesia & Pain Medicine Jan 2007, 32 (1) 60-66; DOI: 10.1016/j.rapm.2006.10.013 ...
https://rapm.bmj.com/keyword/luciferase
Luciferase-based detection of minimal size metastases | Citedby Results | Cancer Research | American Association for Cancer...Luciferase-based detection of minimal size metastases | Citedby Results | Cancer Research | American Association for Cancer...
Luciferase-based detection of minimal size metastases. Cancer Res (2005) 65 (9_Supplement): 903. ...
https://aacrjournals.org/cancerres/crossref-citedby/522646
RePub, Erasmus University Repository: A luciferase-based approach for measuring HBGA blockade antibody titers against human...RePub, Erasmus University Repository: A luciferase-based approach for measuring HBGA blockade antibody titers against human...
A luciferase-based approach for measuring HBGA blockade antibody titers against human norovirus. Publication. Publication. ... 2021). A luciferase-based approach for measuring HBGA blockade antibody titers against human norovirus. Journal of Virological ... Using time-ordered patient sera, the luciferase-based approach was also able to detect changes in HBGA blocking titers ... Methods: To address these issues, a luciferase immunoprecipitation system (LIPS) assay was modified to measure the norovirus- ...
https://repub.eur.nl/pub/136702
Pyrocatechol, a component of coffee, suppresses LPS-induced inflammatory responses by inhibiting NF-κB and activating Nrf2 |...Pyrocatechol, a component of coffee, suppresses LPS-induced inflammatory responses by inhibiting NF-κB and activating Nrf2 |...
NF-κB-dependent luciferase activity was normalized to the activity of constitutively expressed Renilla luciferase. (B) RAW264.7 ... NF-κB-dependent luciferase activity was normalized to the activity of constitutively expressed Renilla luciferase. (B-F) ... NF-κB-dependent luciferase activity was normalized to the activity of constitutively expressed Renilla luciferase. (G) RAW264.7 ... NF-κB-dependent luciferase activity was normalized to the activity of constitutively expressed Renilla luciferase. (H) RAW264.7 ...
https://www.nature.com/articles/s41598-020-59380-x?error=cookies_not_supported&code=970d1222-7b9c-4f10-a5de-fa36a01afc50
Luciferase | Mayim AchronimLuciferase | Mayim Achronim
Tag Archives: Luciferase Genetically-Modified Moses? At the end of this weeks parasha, Ki Tisa, we read how Moses descended ... Luciferase, Midrash Rabbah, Moses, Nails, Pirkei dRabbi Eliezer, Radiation, Skin, Spider Silk, Tree of Knowledge, Zohar on ...
https://www.mayimachronim.com/tag/luciferase/
Establishment of a DGKθ Endogenous Promoter Luciferase Reporter HepG2 Cell Line for Studying the Transcriptional Regulation of...
Shan L, Wang D, Mao Q, Xia H. Establishment of a DGKθ Endogenous Promoter Luciferase Reporter HepG2 Cell Line for Studying the ... Shan, L, Wang, D, Mao, Q & Xia, H 2019, Establishment of a DGKθ Endogenous Promoter Luciferase Reporter HepG2 Cell Line for ... Shan, L., Wang, D., Mao, Q., & Xia, H. (2019). Establishment of a DGKθ Endogenous Promoter Luciferase Reporter HepG2 Cell Line ... Dive into the research topics of Establishment of a DGKθ Endogenous Promoter Luciferase Reporter HepG2 Cell Line for Studying ...
https://www.scholars.northwestern.edu/en/publications/establishment-of-a-dgk%CE%B8-endogenous-promoter-luciferase-reporter-h
Dual-Glo Luciferase Reporter-Gene Assay on the SpectraMax M5, LMax II384 and Analyst GT Microplate ReadersDual-Glo Luciferase Reporter-Gene Assay on the SpectraMax M5, LMax II384 and Analyst GT Microplate Readers
... App Note / Case ... Previously, luciferase reporter assays have been "flash" assays that must be read within seconds of reagent addition and ... See Maxline App Note #39). Recently, Promega introduced a Dual-Glo Luciferase assay system for high-throughput analyses. The ... Bioluminescent reporter systems using firefly and Renilla luciferases are widely used as co-reporters because both assays are ...
https://www.technologynetworks.com/biopharma/application-notes/dualglo-luciferase-reportergene-assay-on-the-spectramax-m5-lmax-ii384-and-analyst-gt-microplate-readers-228666
luciferase reporter Latest Research Papers | ScienceGateluciferase reporter Latest Research Papers | ScienceGate
Find the latest published documents for luciferase reporter, Related hot topics, top authors, the most cited documents, and ... The dual luciferase assay validated the relation of miR-16 with MEK1. U251 cells were cultured and assigned into NC group and ... Using dual-luciferase reporter gene assay to analysis correlation miR-29b-3p and PTEN. Results: miR-29b-3p gene significantly ... Luciferase reporter assay suggested that miR-126-5p was the direct target of lncRNA HOTAIR. Furthermore, co-transfection of si- ...
https://www.sciencegate.app/keyword/88610
Luc-Pair Luciferase Assay Kit 2.0Luc-Pair Luciferase Assay Kit 2.0
Excellent Tools and Services for Life Science Research
https://www1.biocat.com/products/LF003-GC
Characterisation of human melatonin mt(1) and MT2 receptors by CRE-luciferase reporter assay<...Characterisation of human melatonin mt(1) and MT2 receptors by CRE-luciferase reporter assay<...
Characterisation of human melatonin mt(1) and MT2 receptors by CRE-luciferase reporter assay. / Conway, S ; Canning, S J ; ... Characterisation of human melatonin mt(1) and MT2 receptors by CRE-luciferase reporter assay. European Journal of Pharmacology ... Characterisation of human melatonin mt(1) and MT2 receptors by CRE-luciferase reporter assay. In: European Journal of ... A cyclic AMP response element (CRE)-luciferase reporter gene assay was used to characterise the functional responses of human ...
https://abdn.pure.elsevier.com/en/publications/characterisation-of-human-melatonin-mt1-and-mt2-receptors-by-cre-
The Role of the Helper Lipid on the DNA Transfection Efficiency of Lipopolyplex Formulations | Scientific ReportsThe Role of the Helper Lipid on the DNA Transfection Efficiency of Lipopolyplex Formulations | Scientific Reports
Luciferase assay of lung extracts. Finally we determined whether the in vitro results translated to an in vivo application by ... The luciferase expression from LE1PD-0.75 was five-fold higher than LC1PD-0.75 (Fig. 4) while the same formulations ... Luciferase expression in mice lungs was detected 24 h after oropharyngeal instillation of LC1PD-0.75 or LE1PD-0.75 ... Luciferase transfection of LD and LPD nanocomplexes. The transfection efficiency of LC1-3D complexes (4:1 L:D weight ratios) ...
https://www.nature.com/articles/srep07107?error=cookies_not_supported&code=c12a94c8-1aa8-4796-9465-363095a6d362
Luciferase - Silicon ForestLuciferase - Silicon Forest
Luciferase, Quantum Dot, AI Nanotech and the Borgification of Mankind. "Borgification" Greetings from Paul C. Bush, Back in ... 2019 and 2020 The Economic Summit discussed several articles/videos addressing the use of Luciferase, Quantum Dot and AI ...
https://siliconforest.ca/tag/luciferase/
PSA-Luciferase (Neo) lentivirusPSA-Luciferase (Neo) lentivirus
... quantity. Add to cart. SKU: LVP1047-N Categories: Cell-Specific Reporter, Premade Lentivirus, ... Home / Cell-Specific Reporter / Prostate Cell Report / PSA-Luciferase (Neo) lentivirus. View cart "Mito-CFP (Bsd), LocLight ... Pre-made lentivirus express Firefly luciferase 3 reporter under a native human PSA promoter for selective expression in ...
https://www.gentarget.com/product/psa-luciferase-neo-lentivirus/?add-to-cart=8416
Luciferase - Bible Science ForumLuciferase - Bible Science Forum
Posts about Luciferase written by John Gideon Hartnett ... Tag: Luciferase. Categories Book of Revelation Chemistry COVID ... A Luciferase enzyme in the COVID shots causes bioluminescence in people. * Post author By John Gideon Hartnett ... computer brain interface, COVID-19 vaccine, Dr Stella Emmanuel, Great Reset, Human 2.0, Luciferase, Mark of the beast, mRNA ... Bill Gates, Carrie Madej, Coronavirus SARS-Cov-2, COVID-19, COVID-19 vaccine, DNA, Human 2.0, Hydrogel, Luciferase, microneedle ...
https://biblescienceforum.com/tag/luciferase/
Endoplasmic Reticulum Stress Regulates Adipocyte Resistin Expression | Diabetes | American Diabetes AssociationEndoplasmic Reticulum Stress Regulates Adipocyte Resistin Expression | Diabetes | American Diabetes Association
The cells were incubated for 24 additional hours, and luciferase activity was measured by a luciferase assay kit (Promega). ... B: Normalized luciferase activity of resistin-luc (−13,580 bp to +243 bp) or empty vector (pGL3 basic) in the presence of ... B: Normalized luciferase activity of resistin-luc (−13,580 bp to +243 bp) or empty vector (pGL3 basic) in the presence of ... Luciferase assays.. Resistin-luc was generated by inserting −13580/+243 bp fragment of the mouse resistin promoter/enhancer ...
https://diabetesjournals.org/diabetes/article/58/8/1879/15142/Endoplasmic-Reticulum-Stress-Regulates-Adipocyte
Evaluation of chemically modified splice-switching oligonucleotides in an in vitro luciferase assayEvaluation of chemically modified splice-switching oligonucleotides in an in vitro luciferase assay
This thesis is an evaluation of chemically modified splice-switching oligonucleotides in an in vitro luciferase assay. If the ... In the first step the dual-luciferase reporter assay was optimized for the use with oligonucleotides concerning cell-count, ... In total, cellular uptake and consequential up regulation of luciferase genes was proved for the substances conjugated with the ... This reporter assay cell culture model produced an inactive Luciferase enzyme, which could only be activated by splice- ...
https://utheses.univie.ac.at/detail/36135
Joshua Philipp: US Governments Patent Application for Luciferase Fusion Proteins Should Sound Alarm Bells-2022-09-19T23:52:02Z...Joshua Philipp: US Government's Patent Application for Luciferase Fusion Proteins Should Sound Alarm Bells-2022-09-19T23:52:02Z...
... government for fusion proteins containing luciferase, which could be used to track peoples vaccination status. Philipp said it ... US Governments Patent Application for Luciferase Fusion Proteins Should Sound Alarm Bells-2022-09-19T23:52:02Z-Youmaker-NTD ... Joshua Philipp: US Governments Patent Application for Luciferase Fusion Proteins Should Sound Alarm Bells. ... government for fusion proteins containing luciferase, which could be used to track peoples vaccination status. Philipp said it ...
https://www.youmaker.com/v/x0MJl0zB1eYJ_V1-JOSHUA-PHILIPP-SEPT19-2022.html?list=b4ae9caf-ad39-4d27-b364-1fe05ecd8ebc&index=178
Species-independent bioassay for quantification of antiviral type-I interferons based on luciferase-expressing Rift Valley...
Species-independent bioassay for quantification of antiviral type-I interferons based on luciferase-expressing Rift Valley ... Species-independent bioassay for quantification of antiviral type-I interferons based on luciferase-expressing Rift Valley ...
https://www.ndm.ox.ac.uk/publications/813492
FireflyAssaysEnzymeRenillaPTRH1 3UTR Luciferase Stable Cell LineReagentsImmunoprecipitationBioluminescencePromegaHigher luciferase activitySecreted luciferase reporterGenesSubstrateProteinBioluminescentLuciferinCellsReagentProteinsSubstratesHEK293StableInhibitionEndogenousExpressionSystemDrivenVitroSignificantlyCellConformationalDetectDynamicsProductsLightResultsHumanVerify
Firefly21
  • Flash-type luciferase assay designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity. (sigmaaldrich.com)
  • Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. (sigmaaldrich.com)
  • Firefly luciferase is a 62,000 Dalton protein, which is active as a monomer and does not require subsequent processing for its activity. (sigmaaldrich.com)
  • Store Firefly Luciferase Assay at -80°C. Firefly Luciferase Assay Buffer 2.0 is stable at -20°C for three months and at -80°C for at least six months from date of receipt. (sigmaaldrich.com)
  • QuantiLum Recombinant Luciferase is a luciferase expressed from a cloned gene from the North American firefly (Photinus pyralis). (fishersci.com)
  • InvivoGen has engineered a fusion between the firefly luciferase (Luc) and the Sh ble genes. (invivogen.com)
  • LucSh: Synthetic LucSh fusion gene (LucSh-ΔCpG): InvivoGen has engineered a fusion between the firefly luciferase gene and the Sh ble gene conferring Zeocin™ resistance. (invivogen.com)
  • Firefly luciferase is typically used as a readout for expression of a gene of interest. (moleculardevices.com)
  • Bioluminescent reporter systems using firefly and Renilla luciferases are widely used as co-reporters because both assays are easy and sensitive. (technologynetworks.com)
  • Pre-made lentivirus express Firefly luciferase 3 reporter under a native human PSA promoter for selective expression in Prostate tissue or Cells . (gentarget.com)
  • The kit is based on that the firefly luciferase luc gene is one of the most commonly used reporter genes and allows the monitoring of promoter activity in the control of gene expression. (biothema.com)
  • 1) Firefly luciferase is not present in normal cells. (biothema.com)
  • BioThema is entirely focused on analytical applications of the firefly luciferase reaction. (biothema.com)
  • Soluble or biologically localized firefly luciferase is used to continuously monitor ATP concentration either in the bulk solution or at the surface of the organelle, respectively. (bgu.ac.il)
  • INTRODUCTION: As an addition to the second generation recombinant RSVs including RSV-GFP5 (Product #R125), RSV-DsRed5 (#R135), and RSV-FarRed5 (#R155), RSV-Luc5 was created to express Firefly luciferase (Luc) (Promega), the most widely used bioluminescent reporter. (viratree.com)
  • DESCRIPTION: Wild-type parent based on RSV strain A2 were modified to express firefly luciferase (Promega) from a gene cassette placed between the P and M genes. (viratree.com)
  • The open reading frame (ORF) for firefly luciferase gene was PCR amplified to add a ClaI site and an RSV gene start (GS) signal (from the N gene) upstream of the ORF and an RSV gene end (GE) signal (from the G gene) and SalI site downstream of the ORF. (viratree.com)
  • The recovered RSV-Luc5 was further propagated in HEp-2 cells, the expression of firefly luciferase by infected cells was confirmed by bioluminiscence assay, and the sequence of the virus was confirmed in its entirety. (viratree.com)
  • Our cancer cell models are available with one, or a combination, of our four main reporters: enhanced green fluorescent protein (eGFP), firefly luciferase (Fluc), near-infrared fluorescent protein (iRFP), or the sodium-iodide symporter (NIS). (imanislife.com)
  • Examples had been assayed for firefly and renilla luciferase actions using the Dual-Glo Luciferase assay Program (Promega) and normalized as instructed by the product manufacturer. (woofahs.com)
  • UArizona researchers have developed a new split-firefly luciferase enzyme method for measuring protein-protein interactions for assays or sensors. (arizona.edu)
Assays6
  • Luciferase from the sea pansy Renilla reniformis is often used in multiplexed luciferase assays as a second reporter to normalize for transfection efficiency, cell viability, and other factors that may vary among assay samples. (moleculardevices.com)
  • Previously, luciferase reporter assays have been "flash" assays that must be read within seconds of reagent addition and require integrated injectors in the luminometer. (technologynetworks.com)
  • We develop and produce reagents and kits for luciferase based assays and help customers all over the world to set up such assays since our founding in 1987. (biothema.com)
  • In vivo imaging and luciferase assays in tissue lysates revealed that CDCA8 promoter was highly activated and drove robust luciferase expression in testes. (bvsalud.org)
  • This new method may, when combined with previous split luciferase methods, enable assays for two different protein interactions simultaneously, as well as provide a means for additional biophysical characterization. (arizona.edu)
  • Serologic assays for detection of antibodies against EBOV glycoprotein (EBOV-GP), matrix protein (VP40), and nucleoprotein (NP) included novel microneutralization and luciferase immunoprecipitation system (LIPS) assays, as well as a commercially available ELISA. (cdc.gov)
Enzyme7
  • Light emitted from the reaction is directly proportional to the number of luciferase enzyme molecules. (sigmaaldrich.com)
  • This is a potential method to use a glow-under-UV-light Luciferase enzyme in the COVID death shots to keep track on who has and who has not had the shots. (biblescienceforum.com)
  • The name was first used by Raphaël Dubois who invented the words luciferin and luciferase, for the substrate and enzyme, respectively. (biblescienceforum.com)
  • This reporter assay cell culture model produced an inactive Luciferase enzyme, which could only be activated by splice-switching oligonucleotides. (univie.ac.at)
  • Luciferase is a very unique enzyme, derived from fireflies. (carter-heavy-industries.com)
  • In most cases bioluminescence is generated when a light-emitting molecule, called luciferin, chemically reacts with oxygen in the presence of an enzyme, called a luciferase or a photoprotein. (zmescience.com)
  • So in the luciferin system, that we need to have both protein that we called the luciferin, and enzyme, that we called luciferase and oxygen. (coursera.org)
Renilla3
  • Due to its ability to emit light, the luciferase from Renilla reniformis (RLuc) is widely employed in molecular biology as a reporter gene in cell culture experiments and small animal imaging. (rcsb.org)
  • An anisotropic network model highlights the importance of the conformational flexibility of a loop-helix fragment of Renilla luciferases for ligand binding. (muni.cz)
  • We previously developed replication-competent reporter HIV-1 (referred to herein as LucR.T2A reporter viruses), utilizing a "ribosome skipping" T2A peptide strategy to link Renilla luciferase (LucR) with Nef expression. (uab.edu)
PTRH1 3UTR Luciferase Stable Cell Line2
  • Quick contact, quick delivery PTRH1 3UTR Luciferase Stable Cell Line, SMARCA2 3UTR Luciferase Stable Cell Line, Anti-rabbit B cell (subset) monoclonal antibody (clone RTH19A) products, greatests prices. (th1cells.com)
  • The best Human ADAMTS1 CRISPR Knock Out 293T Cell Line, PTH1R 3UTR Luciferase Stable Cell Line, PTRH1 3UTR Luciferase Stable Cell Line products, quick delivery, high quality. (th1cells.com)
Reagents2
  • The results indicated that these reagents could significantly regulate the expression of reporter luciferase. (northwestern.edu)
  • Human IgG antibody Laboratories manufactures the luciferase assay negative control reagents distributed by Genprice. (electroforese.com)
Immunoprecipitation3
  • Methods: To address these issues, a luciferase immunoprecipitation system (LIPS) assay was modified to measure the norovirus-specific HBGA blockade activity of antibodies. (eur.nl)
  • LIPS, luciferase immunoprecipitation system. (cdc.gov)
  • We screened serum samples for neutralizing antibodies by using a pseudotype microneutralization assay and a newly developed luciferase immunoprecipitation system assay. (cdc.gov)
Bioluminescence3
  • Here the authors report a protein-engineering framework based on InDel mutagenesis and fragment transplantation resulting in greater catalysis and longer glow-type bioluminescence of the ancestral luciferase. (muni.cz)
  • Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a photoprotein. (biblescienceforum.com)
  • We developed a generic, highly-sensitive platform for IgG quantification by fusing the IgG-Fc binding Z domain of Staphylococcal Protein A with the ultrabright bioluminescence reporter Nanoluc-luciferase (Nluc). (houstonmethodist.org)
Promega2
  • Recently, Promega introduced a Dual-Glo Luciferase assay system for high-throughput analyses. (technologynetworks.com)
  • Luciferase reporter assay Cells had been transfected with pNF-B-luc (Stratagene, La Jolla, CA) and ether GFP (Clontech, Hill Watch, CA) (sunitinib tests) or pRL-TK (Promega, Madison, WI) (pazopanib tests) plasmids. (woofahs.com)
Higher luciferase activity2
  • Sh fusion gene exhibits a higher luciferase activity and enables a better and faster selection of Zeocin™ resistant clones. (invivogen.com)
  • Melittin-containing polyplexes produced about 35-fold higher luciferase activity in the brain compared to polyplexes without melittin. (mst.edu)
Secreted luciferase reporter1
  • An improved and secreted luciferase reporter for schistosomes. (ucdenver.edu)
Genes2
  • Luciferases are the most common reporter genes, as they are easily detectable using luminometers or luminescence microplate readers, and the low luminescent background present in cells enables high assay sensitivity. (moleculardevices.com)
  • Our expanding catalog includes multiple human and mouse cancer cell line models stably expressing a variety of reporter genes (luciferase, GFP, iRFP, or NIS sodium iodide symporter ) giving you more options to best meet your research needs! (imanislife.com)
Substrate1
  • Carefully lift the rubber cap of the lyophilized BioLux Cypridina Substrate vial and add 60 μl of the ethanol-solvent mixture (for small kit) or 600 μl of the ethanol-solvent mixture (for large kit) to the lyophilized BioLux Cypridina Luciferase Substrate. (neb.com)
Protein2
  • We successfully crystallized a stabilized variant of this important protein (RLuc8) and herein present the first structures for any coelenterazine-using luciferase. (rcsb.org)
  • Here we track the role of dynamics in evolution, starting from the evolvable and thermostable ancestral protein Anc(HLD-RLuc) which catalyses both dehalogenase and luciferase reactions. (muni.cz)
Bioluminescent4
  • The United States Government has patented a new product that uses luciferase and bioluminescent material. (welovetrump.com)
  • The patent was filed by the Department of Homeland Security and the luciferase/bioluminescent product will be a way to "mark" people as a way to verify if they had vaccinations or not. (welovetrump.com)
  • The #DHS filed a patent for a new product using luciferase, a bioluminescent material that is being proposed to mark people as a way to verify if they've had vaccinations. (welovetrump.com)
  • Typically, the synthesized bioluminescent probes are made of glucose polymer (GP), luciferase, Cy5 and ICG-modified silicon nanoparticles and their substrates are made of GP and D-luciferin-modified silicon nanoparticles. (bvsalud.org)
Luciferin1
  • Herein, we leverage bacteria-specific ATP-binding cassette sugar transporters to internalize luciferase and luciferin by hitchhiking them on the unique carbon source of bacteria. (bvsalud.org)
Cells6
  • It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues. (sigmaaldrich.com)
  • BOMBSHELL: Pfizer whistleblower says vaccine 'glows,' contains 'toxic' luciferase, graphene oxide compounds Melissa Strickler, a Christian, recently went public with insider emails showing Pfizer executives wanted to hide the vaccine's connection to aborted fetal cells. (maithaigraphene.com)
  • Transcription factor activation was assessed using HASM cells transduced with luciferase reporter gene constructs. (ersjournals.com)
  • Hamster tracheal epithelial (HTE) cells were transfected with the reporter plasmid jun- luciferase and the empty expression vector Rous sarcoma virus (RSV- O). The treated cells were harvested 16 or 40 hours (hr) after exposure to crocidolite (12001284) asbestos , hydrogen-peroxide (7722841) (H2O2), or the tumor promoter 12-O-tetradecanoylphorbol-13- acetate (TPA). (cdc.gov)
  • Furthermore, GL331-induced down-regulation of mmp-9 gene promoter was demonstrated by using a luciferase reporter gene driven by the -216 to -13 region of the mmp-9 gene promoter cloned from CL1-5 cells. (elsevier.com)
  • The CL1-5 cells transfected with the luciferase reporter containing 18 d(CA)s expressed only 53% of those when the reporter contained 24 d(CA)s. (elsevier.com)
Reagent1
  • The Luciferase Assay Negative Control reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (electroforese.com)
Proteins2
  • NTD spoke with Crossroads host Joshua Philipp about a patent filed by the U.S. government for fusion proteins containing luciferase, which could be used to track people's vaccination status. (youmaker.com)
  • Polynucleotides encoding fusion proteins contain a secretable luciferase fused to a modified polypeptide of interest are disclosed. (welovetrump.com)
Substrates1
  • The luciferases from different organisms have evolved differently so have different structures and substrates. (bvsalud.org)
HEK2931
  • A cyclic AMP response element (CRE)-luciferase reporter gene assay was used to characterise the functional responses of human melatonin mt(1) and human melatonin MT2 receptors, stably expressed in the human embryonic kidney cell line HEK293, to a series of six naphthalenic analogues of melatonin. (elsevier.com)
Stable2
  • To further investigate the molecular mechanism of this inhibition, we generated a stable reporter cell line that expresses luciferase under the control of a TNF-alpha promoter region. (cdc.gov)
  • Description: Luciferase (firefry) stable cell line with Blasticidin marker. (choleratox.com)
Inhibition1
  • By comparison to the observed melatonin-mediated inhibition of stimulated luciferase levels the naphthalenic series was identified as comprising agonists, partial agonists and one antagonist of melatonin mt(1) and melatonin MT2 receptor function. (elsevier.com)
Endogenous3
  • To investigate the transcriptional regulation of the DGKθ gene, we used CRISPR/Cas9 to generate a DGKθ endogenous promoter luciferase reporter HepG2 cell line, in which the endogenous DGKθ promoter controls the expression of the luciferase reporter gene. (northwestern.edu)
  • We found that the cell line's luciferase activity was directly correlated with DGKθ endogenous promoter activity, suggesting that it is liable and sensitive for studying DGKθ transcriptional regulation. (northwestern.edu)
  • Direct connections between miRs and was driven in the luciferase assay and additional verified in cell lines, where overexpression of miR-146a-5p and miR-146b-5p triggered a 31% and 33% reduction in endogenous mRNA amounts. (euromed2016.com)
Expression7
  • The luminescence signal decays over the course of about 10 minutes of reaction time, although signal half-life may vary depending on luciferase expression levels. (sigmaaldrich.com)
  • The result of the Dual-luciferase reporter assay revealed that CASC7 directly targeted miR-340-5p and negatively regulated its expression. (sciencegate.app)
  • We aimed to investigate the mechanism by which EGCG promotes skin hydration by measuring hyaluronic acid synthase ( HAS ) and hyaluronidase ( HYAL ) gene expression and antioxidant and anti-pigmentation properties using cell proliferation assay, Western blotting analysis, luciferase assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, and reverse transcription polymerase chain reaction (RT-PCR) analysis. (mdpi.com)
  • Furthermore, diphenols such as para-hydroquinone, catechol, and resorcinol also inhibit LPS-induced luciferase expression. (cdc.gov)
  • QRT-PCR and luciferase reporter assay were used to verify the expression and target relationship of hsa_circ_0088196/LINC01492/miR-100-5p/LIF (leukemia inhibitory factor). (medscimonit.com)
  • Dual-luciferase report assay showed that miR-325-3p directly targeted Trp53 to down-regulated its expression. (bvsalud.org)
  • Luciferase reporter system for studying the effect of nanoparticles on gene expression. (cdc.gov)
System1
  • One key component of that is the micro-needle delivery system, when the substance called Luciferase is added, it produces under the skin, an array of quantum (nano-sized) dots. (biblescienceforum.com)
Driven3
  • We found that GL331 did not significantly influence the luciferase activity driven by TRE or κB. (elsevier.com)
  • Here, we studied the CDCA8 transcription in adult tissues by generating a transgenic mouse model, in which the luciferase was driven by a 1-kb human CDCA8 promoter. (bvsalud.org)
  • Release of rpL11 from the ribosome by serum deprivation or low-dose actinomycin D did not dramatically affect PPRE-driven luciferase activity when PPARα was overexpressed by cotransfection. (elsevier.com)
Vitro1
  • Diese Arbeit beschäftigt sich mit der Evaluierung von chemisch modifizierten splice-switching Oligonukleotiden in einem in vitro Luciferase Assay. (univie.ac.at)
Significantly1
  • Significantly increased luciferase activity was detected after 16 and 40hr exposure to 2.5 or 5.0 micrograms/cubic centimeter asbestos , 10 micromolar H2O2, or 100 nanograms/milliliter TPA. (cdc.gov)
Cell3
  • In the first step the dual-luciferase reporter assay was optimized for the use with oligonucleotides concerning cell-count, incubation time and concentration and selection of control substances. (univie.ac.at)
  • Our results show that LPS induces luciferase production and tBHQ totally blocks this induction in the cell. (cdc.gov)
  • We generated luciferase- and mCherry-expressing derivatives of a subset of those murine osteosarcoma cell lines, to monitor tumor growth and drug responses in vivo. (safe-crypto.me)
Conformational1
  • We also present data of a coelenteramide-bound luciferase and reason that this structure represents a secondary conformational form following shift of the product out of the primary active site. (rcsb.org)
Detect1
  • Using time-ordered patient sera, the luciferase-based approach was also able to detect changes in HBGA blocking titers following viral challenge and natural infection with norovirus. (eur.nl)
Dynamics1
  • LUCiFerAse real-time reporting of circadian dynamics reveals persistent circadian oscillations in mouse peripheral tissues. (medscape.com)
Products1
Light1
  • The Luciferase allows the dots to fluoresce when illuminated with the correct wavelength of light. (biblescienceforum.com)
Results1
  • The results showed up-regulation of luciferase in combination with lipofectamine in eight out of eight oligolysine conjugated oligonucleotides but only in four out of seven with PEG-conjugation. (univie.ac.at)
Human2
  • Competitive displacement of 2-[I-125]iodomelatonin binding to the ovine pars tuberalis melatonin ML1 receptor demonstrated a close correlation to the observed functional luciferase responses of the human melatonin mt(1) receptor. (elsevier.com)
  • We conclude that the CRE-luciferase reporter gene assay provides an effective functional screening method for the pharmacological characterisation of human melatonin receptor subtypes. (elsevier.com)
Verify1
  • Dual-Luciferase reporter gene assay was performed to verify the relationship between circ_0010452 and miR-543. (sciencegate.app)
Pyrocatechol, a component of coffee, suppresses LPS-induced inflammatory responses by inhibiting NF-κB and activating Nrf2 |...
Pyrocatechol, a component of coffee, suppresses LPS-induced inflammatory responses by inhibiting NF-κB and activating Nrf2 |... (nature.com)
The β2-adrenergic receptor antagonist ICI-118,551 blocks the constitutively activated HIF signalling in hemangioblastomas from...
The β2-adrenergic receptor antagonist ICI-118,551 blocks the constitutively activated HIF signalling in hemangioblastomas from... (nature.com)
MeCP2 inhibits ischemic neuronal injury by enhancing methylation of the FOXO3a promoter to repress the SPRY2-ZEB1 axis |...
MeCP2 inhibits ischemic neuronal injury by enhancing methylation of the FOXO3a promoter to repress the SPRY2-ZEB1 axis |... (nature.com)
Selective targeting of the TLR2/MyD88/NF-κB pathway reduces α-synuclein spreading in vitro and in vivo | Nature Communications
Selective targeting of the TLR2/MyD88/NF-κB pathway reduces α-synuclein spreading in vitro and in vivo | Nature Communications (nature.com)
Nrf2 suppresses macrophage inflammatory response by blocking proinflammatory cytokine transcription | Nature Communications
Nrf2 suppresses macrophage inflammatory response by blocking proinflammatory cytokine transcription | Nature Communications (nature.com)
The Arabidopsis NPF3 protein is a GA transporter | Nature Communications
The Arabidopsis NPF3 protein is a GA transporter | Nature Communications (nature.com)
Endoplasmic Reticulum Stress Regulates Adipocyte Resistin Expression | Diabetes | American Diabetes Association
Endoplasmic Reticulum Stress Regulates Adipocyte Resistin Expression | Diabetes | American Diabetes Association (diabetesjournals.org)
The Role of the Helper Lipid on the DNA Transfection Efficiency of Lipopolyplex Formulations | Scientific Reports
The Role of the Helper Lipid on the DNA Transfection Efficiency of Lipopolyplex Formulations | Scientific Reports (nature.com)
PDF) Suppression of Poxvirus Replication by Resveratrol
PDF) Suppression of Poxvirus Replication by Resveratrol (researchgate.net)
Frontiers | A Testosterone Metabolite 19-Hydroxyandrostenedione Induces Neuroendocrine Trans-Differentiation of Prostate Cancer...
Frontiers | A Testosterone Metabolite 19-Hydroxyandrostenedione Induces Neuroendocrine Trans-Differentiation of Prostate Cancer... (frontiersin.org)
Frontiers | CircRNA circ_POLA2 Promotes Cervical Squamous Cell Carcinoma Progression via Regulating miR-326/GNB1
Frontiers | CircRNA circ_POLA2 Promotes Cervical Squamous Cell Carcinoma Progression via Regulating miR-326/GNB1 (frontiersin.org)
Development of a dual hybrid AAV vector for endothelial-targeted expression of von Willebrand factor | Gene Therapy
Development of a dual hybrid AAV vector for endothelial-targeted expression of von Willebrand factor | Gene Therapy (nature.com)
Plus it
Plus it (erj.ersjournals.com)
Niko Koronis - Suku 1 Green - Carpenters Workshop Gallery - Design Miami/ The global forum for collectible design
Niko Koronis - Suku 1 Green - Carpenters Workshop Gallery - Design Miami/ The global forum for collectible design (designmiami.com)
IJMS | Free Full-Text | Skin Protective Effect of Epigallocatechin Gallate
IJMS | Free Full-Text | Skin Protective Effect of Epigallocatechin Gallate (mdpi.com)
Frontiers | MicroRNA-34a Inhibition Alleviates Lung Injury in Cecal Ligation and Puncture Induced Septic Mice
Frontiers | MicroRNA-34a Inhibition Alleviates Lung Injury in Cecal Ligation and Puncture Induced Septic Mice (frontiersin.org)
What Is Lawful Money & Who Stole Yours? Must See Video! | Alternative | Before It's News
What Is Lawful Money & Who Stole Yours? Must See Video! | Alternative | Before It's News (beforeitsnews.com)
Mapping long-range promoter contacts in human cells with high-resolution capture Hi-C | Nature Genetics
Mapping long-range promoter contacts in human cells with high-resolution capture Hi-C | Nature Genetics (nature.com)
Scholars' Day Home
Scholars' Day Home (ramapo.edu)
Systematic characterization of seed overlap microRNA cotargeting associated with lupus pathogenesis | BMC Biology | Full Text
Systematic characterization of seed overlap microRNA cotargeting associated with lupus pathogenesis | BMC Biology | Full Text (bmcbiol.biomedcentral.com)
Β-actin-luc | Mouse Models | Taconic Biosciences
Β-actin-luc | Mouse Models | Taconic Biosciences (taconic.com)
Comprehensive exploration of chemical space using trisubstituted carboranes | Scientific Reports
Comprehensive exploration of chemical space using trisubstituted carboranes | Scientific Reports (nature.com)
Der FFP2-Masken-Skandal | Gutscheine für Tote und Millionen für Apotheker - gloria.tv
Der FFP2-Masken-Skandal | Gutscheine für Tote und Millionen für Apotheker - gloria.tv (gloria.tv)
Scientist added jellyfish genes to crap fish DNA and these glowing fish are the result : Damnthatsinteresting
Scientist added jellyfish genes to crap fish DNA and these glowing fish are the result : Damnthatsinteresting (reddit.com)
Davos In The Catacombs: Klaus Schwab's Secret Vatican Connection! Mystery Babylon - Satan In Church Today - The False Prophet...
Davos In The Catacombs: Klaus Schwab's Secret Vatican Connection! Mystery Babylon - Satan In Church Today - The False Prophet... (beforeitsnews.com)
In Order To Understand Revelation, We Have to Go Back To The Beginning In Genesis. There Were 2 Creations of the White Race in...
In Order To Understand Revelation, We Have to Go Back To The Beginning In Genesis. There Were 2 Creations of the White Race in... (beforeitsnews.com)
Angelina Jolie Joins The Illuminati! Must See Video! | Alternative | Before It's News
Angelina Jolie Joins The Illuminati! Must See Video! | Alternative | Before It's News (beforeitsnews.com)
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Share (ajtmh.org)
Discover Glo 2021
Discover Glo 2021 (promega.com)